LOCUS       DAZPJR010000001       108101 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPJR010000001 DAZPJR010000000
VERSION     DAZPJR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 108101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 108101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..108101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.001"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(33..563)
                     /locus_tag="ACHVB7_000001"
     CDS             complement(33..563)
                     /locus_tag="ACHVB7_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR1912118.1"
                     /translation="MSEINYQALREVAERAIPAMERLLMLPADDDLLSEQELKDSGVD
                     IDALNAFKFLAGPETVLALLDEINALEETRINDVCRIAELTKQLELAKSKLNEQREYY
                     EGVISDGSKRIAELESDSQAQKLVEAIIVAIENEQERLFDEDYLMDSKECIDVIREEV
                     KRWNDSRAAGIRIKGE"
     gene            complement(550..804)
                     /locus_tag="ACHVB7_000002"
     CDS             complement(550..804)
                     /locus_tag="ACHVB7_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912119.1"
                     /translation="MSNSARLQLGFSPLSKTIMLAKMRDVEGGRMRVGNDPGRDVTNE
                     AAQLVWQLVMAEGGEIVWELDDGSRMVLKAEKQEAASERN"
     gene            complement(801..1343)
                     /locus_tag="ACHVB7_000003"
     CDS             complement(801..1343)
                     /locus_tag="ACHVB7_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912120.1"
                     /translation="MSNKIENPVVLIHKRENHDSYEVAITNGSHDYYDGLLMASVSPD
                     EADNSFAVFAMVGYYMAAEIEKLRAQRDALAAENAALKESERAFDAMCAEEHGDNWVS
                     ELTETPATDAFLAEVRAQGVDAAIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLVGK
                     VEMTEWLVDFAAQLRKGGNQ"
     gene            complement(1482..1862)
                     /locus_tag="ACHVB7_000004"
     CDS             complement(1482..1862)
                     /locus_tag="ACHVB7_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912121.1"
                     /translation="MFGIDAQRIAAFAKSPLDNPLSRSEQMELARLFLHIQKQADIFN
                     NMSNQPILDGHIQMVINSHEKGWAAIVPCTITYRLAKEVQEFRKASVESESTKAAINT
                     LIRMGFTWTGGAYWQAPHPILYGN"
     gene            complement(1862..2566)
                     /locus_tag="ACHVB7_000005"
     CDS             complement(1862..2566)
                     /locus_tag="ACHVB7_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912122.1"
                     /translation="MNNLQLEHFNVTGHSDFPFKFTLKGYAEDAVGQIIIDKGIVKFE
                     GDFDESAKTFIDFIAKRWSEQWKDLEKRANEFDRFMDTMDTAKEALAAGTPLDLESLF
                     NSTMASEMFATMFAGEFVRSGAKNYLELDYNVPAIGDFVVTIQREEGKTPGERVAELE
                     AVVDQRNGECDRLINELHALREERICAGSNTRNAADIYFQLVEECQIPPGGSLVDYVR
                     HLMAEVNSSHKDGEVR"
     gene            complement(2628..4313)
                     /locus_tag="ACHVB7_000006"
     CDS             complement(2628..4313)
                     /locus_tag="ACHVB7_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR1912123.1"
                     /translation="MNTALSIMGDASSNTAIDYRQEMNVIHEIVAECEKEISFMYQVH
                     DFVYGDERHNMINRLLRLNHRPDEDRSRLNRVWLDNVDLEWVKQNIWAEYWRKVTDMT
                     NVLLIMPASRRDEWRELFIEGKQEVIKTDRTGYQMKVKEFVGVPEFKAETVIPTMLNL
                     LNDRHKYLSERVYGLFKALSPAHKTNKTNGFSERLIIADCISDFWRDSVSVNYRKEDY
                     IDDLRVLLHFFAHKEFITINRTAEVLSAAYRANDCQTGDWMNVDGNLMRVKMFKNGNV
                     HFEIHPDVAWKLNEVLAYSMPAAIPAPCRTAPKTRAPKQFGLIQKTISVPVRTALRDG
                     RLSKDKGVWYFSDSALQKSQVEELERTLSFIGGVQEKKHWQFPYEIGHTLNTIVATGL
                     IPDTKSHQFYPTPRLIAEYVARATELKPGEKLLEPQAGRGDLLAYINADLEDVTCIEI
                     APLFADILRGKGYTNTICCDFIKWSEDNAGYQFDKIVMNPPYSLGRHREHTLAALGHL
                     KVGGRLVAVLPGTAPILDWMTMDNYVYARGKSFTNEFEDTGITVSVYVFKRVK"
     gene            complement(4417..5031)
                     /locus_tag="ACHVB7_000007"
     CDS             complement(4417..5031)
                     /locus_tag="ACHVB7_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912124.1"
                     /translation="MFGMSITQSNGNVWMSPEFTPQNLINRGTMSTSKGSVFKTSIPS
                     NKSCFFFIKSSNKANMMFVHEFRDGYNVLRLNQVGGNPGTITVYAFSDMVLPHSGYGI
                     AMYNSAGKMVYHGEMMPLDAEIITITNPQFTINMGYPCAIMPAMVGVYNYRRTPYDRP
                     VYVTMTGATGNQIYNGEWYSSNVTWNVKKLYTNKVLVINTSKYD"
     gene            complement(5037..5318)
                     /locus_tag="ACHVB7_000008"
     CDS             complement(5037..5318)
                     /locus_tag="ACHVB7_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912125.1"
                     /translation="MAYGTGIYNNKGVNVTGFLTPIFFLDRFTTTSGSKTYPNPPPGK
                     SLHAVCSLMPLNNDNHVNIPTPTITINGNTVSWSNLYKGLGSYIYTYWG"
     gene            complement(5373..5942)
                     /locus_tag="ACHVB7_000009"
     CDS             complement(5373..5942)
                     /locus_tag="ACHVB7_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912126.1"
                     /translation="MCEKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERVV
                     TALNFESTFEEPTQVVALAHHFGVHYLAEKQRADKLQETLDMVIETQGTHDSNKSETI
                     IASKDREIAGLKSSLTMLMSAFNLMSSQAGYKMPSLNSDDPMVVRQLLGAMADQLDDT
                     KSRLEDMMRELSHRHNLATQPHKAFQSSN"
     gene            complement(6082..6240)
                     /locus_tag="ACHVB7_000010"
     CDS             complement(6082..6240)
                     /locus_tag="ACHVB7_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR1912127.1"
                     /translation="MLERFRLVTVIALLVMAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKATC"
     gene            complement(6240..6665)
                     /locus_tag="ACHVB7_000011"
     CDS             complement(6240..6665)
                     /locus_tag="ACHVB7_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite/colicin resistance protein"
                     /protein_id="HFR1912128.1"
                     /translation="MLGFFKKKTRKAVIEVKKMENRDAVEATVWGGYMISYANGTCDA
                     KEISILEKTIAALPAFSPFAGEIAQMSANIRAQYEASPRRANAQALRELADVAGTDDA
                     VDVLCLCLDIADQDGIDEPEEQALKKIAQALQLSLDAYL"
     gene            complement(6759..6947)
                     /locus_tag="ACHVB7_000012"
     CDS             complement(6759..6947)
                     /locus_tag="ACHVB7_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912129.1"
                     /translation="MNWFSNHFGKIWLAILALMAAGWVSNIIKLVCSGDLQFQAGMTL
                     ARVVGIFIFPVGSVLGYF"
     gene            complement(6957..7451)
                     /locus_tag="ACHVB7_000013"
     CDS             complement(6957..7451)
                     /locus_tag="ACHVB7_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912130.1"
                     /translation="MARQTYFTSVTKRPRSLRQILAELFSGRVMSRLDELETTVRLLN
                     ERLDNQASVVANVWAIVASGSSREAKNARPLVKEKNNKDSSNGKFSKKEAETNGLRSH
                     YSFTGDGSRSSRSEPFDAGFIHHHTSVDDNYHHSSRATCHSGWDDGGCDTSSSSSYSG
                     SCCD"
     gene            complement(7597..8190)
                     /gene="rnhA"
                     /locus_tag="ACHVB7_000014"
     CDS             complement(7597..8190)
                     /gene="rnhA"
                     /locus_tag="ACHVB7_000014"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1912131.1"
                     /translation="MTTKTPARPQAKTRKKDKNKNSPRTNPTTPVVEFNPQLKTVKIF
                     SDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRMEMMGALIGLERLKYPCNV
                     ILHSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWKRLDEAASRHNVRWKWVKG
                     HAGHRENEICDRLAKIAAFSAADMPHKKDIGFVYNKQ"
     gene            complement(8772..9002)
                     /locus_tag="ACHVB7_000015"
     CDS             complement(8772..9002)
                     /locus_tag="ACHVB7_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912132.1"
                     /translation="MSYPTNVVALVDSDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSK"
     gene            complement(9190..9783)
                     /locus_tag="ACHVB7_000016"
     CDS             complement(9190..9783)
                     /locus_tag="ACHVB7_000016"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFR1912133.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGIERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATEYANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
     gene            complement(9966..10775)
                     /locus_tag="ACHVB7_000017"
     CDS             complement(9966..10775)
                     /locus_tag="ACHVB7_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912134.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESGEAAENEPEQL
                     GIIEVEDCVEVNDELKVQQSNDSNTNKKTRAARGPSFTLSDKDDSFFNKAGLEKDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            complement(10936..11493)
                     /locus_tag="ACHVB7_000018"
     CDS             complement(10936..11493)
                     /locus_tag="ACHVB7_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFR1912135.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMEACSAWVAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            complement(11503..11922)
                     /locus_tag="ACHVB7_000019"
     CDS             complement(11503..11922)
                     /locus_tag="ACHVB7_000019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912136.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            complement(11984..12628)
                     /locus_tag="ACHVB7_000020"
     CDS             complement(11984..12628)
                     /locus_tag="ACHVB7_000020"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1912137.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            complement(12628..13104)
                     /locus_tag="ACHVB7_000021"
     CDS             complement(12628..13104)
                     /locus_tag="ACHVB7_000021"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR1912138.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            complement(13101..13514)
                     /locus_tag="ACHVB7_000022"
     CDS             complement(13101..13514)
                     /locus_tag="ACHVB7_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912139.1"
                     /translation="MARGMYVLCEIEGVLANTSHRKSVPDTDAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            complement(13516..14619)
                     /locus_tag="ACHVB7_000023"
     CDS             complement(13516..14619)
                     /locus_tag="ACHVB7_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1912140.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQTDDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRFGYESIYTKMLYDLEMDGSVTETELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDDILILGYDPLPWIKVPVAV"
     gene            complement(14787..15656)
                     /locus_tag="ACHVB7_000024"
     CDS             complement(14787..15656)
                     /locus_tag="ACHVB7_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFR1912141.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            complement(15734..16876)
                     /gene="nrdB"
                     /locus_tag="ACHVB7_000025"
     CDS             complement(15734..16876)
                     /gene="nrdB"
                     /locus_tag="ACHVB7_000025"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1912142.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWAEYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            complement(16982..19297)
                     /gene="nrdA"
                     /locus_tag="ACHVB7_000026"
     CDS             complement(16982..19297)
                     /gene="nrdA"
                     /locus_tag="ACHVB7_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1912143.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGMTTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQELFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGIRYSDTAGNKLVHETFEAIQYYLLDASCQLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEIEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            complement(19371..19940)
                     /locus_tag="ACHVB7_000027"
     CDS             complement(19371..19940)
                     /locus_tag="ACHVB7_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912144.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHDLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHSKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            complement(19950..20693)
                     /locus_tag="ACHVB7_000028"
     CDS             complement(19950..20693)
                     /locus_tag="ACHVB7_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912145.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHENEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NSPQPTLQTAIVTNSLEARLLENPIWGTW"
     gene            complement(20683..22599)
                     /locus_tag="ACHVB7_000029"
     CDS             complement(20683..22599)
                     /locus_tag="ACHVB7_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1912146.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEIVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            complement(22829..23914)
                     /locus_tag="ACHVB7_000030"
     CDS             complement(22829..23914)
                     /locus_tag="ACHVB7_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFR1912147.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            complement(24169..24813)
                     /locus_tag="ACHVB7_000031"
     CDS             complement(24169..24813)
                     /locus_tag="ACHVB7_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912148.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            25015..26229
                     /locus_tag="ACHVB7_000032"
     CDS             25015..26229
                     /locus_tag="ACHVB7_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFR1912149.1"
                     /translation="MLSAVIDSLSAVHRMLPTVFGILSAANQVCCQRLNDKGVHMSTK
                     NKKGKNNKEVEDNIDNFEEGSLELYTGELVPNSNNTVQPIALMRLGLFVPTLKGTKYS
                     KRNKPNEIDASKELVQLEVARSEGYSDIKITGPRLDMDHDFKTWVGVVRSLAEYGEPN
                     GRVELSITKFAKFCGYPSSQIRKTLRDRLTNSLLKIMRTTLSFQRTYEEKNVDGSNKI
                     SLLMVHLINSVDYNEQKDTVVFYAEPKLAELYRFDHKVLLQLKVINKLPRKETAQALY
                     TFIESLPTKPAPVSLARLRARLNLSSRNVSSQNQTIRNGLKALQDLGYLEYSEIKRGR
                     SIYIQIHSRNPKLKVAPPKPEDIEPKKPDEKAGEIDAKQNIINKITELSQNLTPENIK
                     MIEILSNSLKLL"
     gene            complement(26809..27021)
                     /locus_tag="ACHVB7_000033"
     CDS             complement(26809..27021)
                     /locus_tag="ACHVB7_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912150.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(27021..27356)
                     /locus_tag="ACHVB7_000034"
     CDS             complement(27021..27356)
                     /locus_tag="ACHVB7_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912151.1"
                     /translation="MKTIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            complement(27572..27847)
                     /locus_tag="ACHVB7_000035"
     CDS             complement(27572..27847)
                     /locus_tag="ACHVB7_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912152.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKVDGLNDEETDALIQRTLVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISNEDAEHLERILSISTFE"
     gene            complement(27903..28328)
                     /locus_tag="ACHVB7_000036"
     CDS             complement(27903..28328)
                     /locus_tag="ACHVB7_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912153.1"
                     /translation="MAAIKKLYDAANVALDVIDDEVAKGFPEPDWAHQLRNAIAEMTP
                     PDPTPDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPNQED"
     gene            complement(28390..28779)
                     /locus_tag="ACHVB7_000037"
     CDS             complement(28390..28779)
                     /locus_tag="ACHVB7_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912154.1"
                     /translation="MLLIQPGFGLKIKKGHMFGERDSKRKILSIRLPFITIHWLNRES
                     TDYWYKCARAAFNDPDWFVENHHAVRQAKRKANTTYMKAYQDAWEEHRGRYQKNIEKL
                     ESENMDLRRKLGEARRDIEAYKRLVSE"
     gene            complement(28800..29540)
                     /locus_tag="ACHVB7_000038"
     CDS             complement(28800..29540)
                     /locus_tag="ACHVB7_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912155.1"
                     /translation="MKRKIEEPKTTLNKHGEKIQTHPAFGLVKTSRVSTTGIRLFDSE
                     LEHRDYIEIGVYEAELSLDNERTRPQRSCNRRPLVEIRLSQAQWAAMVSSFGVGEGVP
                     CTISYKTIGDSIRVHDIAEQKSIRQKFNDQIKAATNSEIDRLNQQVSELGELVKKGRA
                     GKRDLEELYRKLASAVANLPENLCFATELMQEQMDEIVSSGKAEIEAYVAGTVMRKGL
                     AALGTESCINDDPTVSITKMESGKELLP"
     gene            complement(29588..30412)
                     /locus_tag="ACHVB7_000039"
     CDS             complement(29588..30412)
                     /locus_tag="ACHVB7_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912156.1"
                     /translation="MPNHITNEIRVIGGTNKQRLDFIRAITNKKGLIDFNTICRRPKS
                     IEMEENNHIRRMASAMAGETVYDHMFGELKTPEQVELAMRESGMTRKEIRKIKEQALM
                     RLENYRRYGYYSWFDWSRANWGTKWNAYNIEMPVKRIPTRIKYGHNFRHTHVRAYAKR
                     IYKKRLARHAATGGELVIRFETAWSMPKPIYQAMARKFPHLEFVIRYADEDFGNNCGF
                     ILLRNGKWEYDYIAPANNKQSSEEKIKWRKFAFELCCPGITPQEYGLNEEYEYAGY"
     gene            complement(30508..31338)
                     /locus_tag="ACHVB7_000040"
     CDS             complement(30508..31338)
                     /locus_tag="ACHVB7_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1912157.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            complement(31342..31542)
                     /locus_tag="ACHVB7_000041"
     CDS             complement(31342..31542)
                     /locus_tag="ACHVB7_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912158.1"
                     /translation="MIKIYLLAVATGVSVALIYGLLVPSLISTKSDLAVMFGVIVGFG
                     APVIGLIAGRKFINSLIKAKGK"
     gene            complement(31635..32708)
                     /locus_tag="ACHVB7_000042"
     CDS             complement(31635..32708)
                     /locus_tag="ACHVB7_000042"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1912159.1"
                     /translation="MAKGKSALALALKKKIGSNDEIQKVTHWIDTGFPPLNKAISGRY
                     DGGFPCGRIVEVFGPPSAGKTFLATAAMISAQKQDGLAVFLDHENSFDVGLAVANGLN
                     ADEDDGQWVYKQPDTFEDSVELIGTILKLVRDEELIPESAPICIVADSLASMVPNSKA
                     EKFEKMAEGTAKDKDQLNMNDNTALARATSANFPTLALWARKYNACIIFLNQVRTKIG
                     VMFGDPTTSPGGDSPKFYASVRIRLGASVMKDGKEKIGQDVGAECIKNKVAPPYGKCT
                     WKFYFDPTRGLDVIESLVEYMLEEGYLPKNASGRVEIGDKKYTKSQIVEMYREKPLAE
                     IIAALQAIDDRRAKDNPTESVEE"
     gene            complement(32711..32977)
                     /locus_tag="ACHVB7_000043"
     CDS             complement(32711..32977)
                     /locus_tag="ACHVB7_000043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912160.1"
                     /translation="MLKSIINGATTTPTQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            complement(32977..33921)
                     /locus_tag="ACHVB7_000044"
     CDS             complement(32977..33921)
                     /locus_tag="ACHVB7_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HFR1912161.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVASIAPQPIVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDKSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVDKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            complement(33982..35010)
                     /locus_tag="ACHVB7_000045"
     CDS             complement(33982..35010)
                     /locus_tag="ACHVB7_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFR1912162.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVAAASATEDEGLDD
                     LLRELDSL"
     gene            complement(35128..35559)
                     /locus_tag="ACHVB7_000046"
     CDS             complement(35128..35559)
                     /locus_tag="ACHVB7_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912163.1"
                     /translation="MITDIYEKIMSDLEFDRDNLEEVWRRQPRLLMEYGSKLAHAERS
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDDARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            complement(35685..39203)
                     /gene="dnaE"
                     /locus_tag="ACHVB7_000047"
     CDS             complement(35685..39203)
                     /gene="dnaE"
                     /locus_tag="ACHVB7_000047"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1912164.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRDKLAKESKGCMESLERGRNYSFTALIK
                     NEQGYRDICELMTVANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITPGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAVNGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDTIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELKNVEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDTISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPESLHPVLD
                     ELTAETNGVILYQEQTMNATRLLAGFTMAEADGVRKAIGKKDMEKMKSMGEKFIVQAQ
                     AGWIDVELEDGTTQRIHRAEHFKCEDGTMKTVEEALEYGAKLPINAVRVTASHPGLSE
                     MKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQVLYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESLAQFEEAVEKRACNSRVRESLQKVGAFASIEPGSLPATDPERLRDQAE
                     LMGNLVIDAVKASRPFEMNSKRSAEINVLMTRMAAEMGLGDELIRPSIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTAGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFMREEINLVRPTYVLTCGSRATALFNNKNKPSDLVGRKEYLPDLDVTVFYGFNP
                     NILYFRPEEGERLEAILADVAETIKS"
     gene            complement(39384..40619)
                     /locus_tag="ACHVB7_000048"
     CDS             complement(39384..40619)
                     /locus_tag="ACHVB7_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1912165.1"
                     /translation="MTTTALQNEKNPSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGLTSSPGYTGTVE
                     YKTDLVAAHELLNVTVEELGTKRGTPLMVSVNVNTPYPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKDAEINMLTNA
                     GIVDEYAEKMVKFAGIVRDGYEEHNISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            complement(40715..42823)
                     /locus_tag="ACHVB7_000049"
     CDS             complement(40715..42823)
                     /locus_tag="ACHVB7_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HFR1912166.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSRSLG
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDADQTDASGENNEDNEDHETPSALDNHKSIESES
                     HSKHKHDNNDSDDSDNSESSETIFDDTENDKEVSDSDASDNAASESLTADHEKRKTTE
                     DGSSDIPTPSKMSLEEALEELDSIEDEVGGMTEDALSETIKSELTESSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLAMNDDRVFRKKEESKAVNACVQQ
                     VIDLSGSMSGITIQLALASSYTIADALDRINVPNIITGFTTFGSHMAAGELKAVKYEF
                     SRFESLMLPIIKNWNEKVSSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRMED
                     RKIMLVLSDGAPWAVGRGFDAHLRSVAKQIETQTDIDLMAIGIMTDAPERFYSNHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            complement(42922..43134)
                     /locus_tag="ACHVB7_000050"
     CDS             complement(42922..43134)
                     /locus_tag="ACHVB7_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912167.1"
                     /translation="MKTTISSLITLEIGHVEKLADECVADILTELPNEQIQVGMHDAT
                     AFIFLLNNKRFTLINTGSGSLAVRTC"
     gene            43382..43768
                     /locus_tag="ACHVB7_000051"
     CDS             43382..43768
                     /locus_tag="ACHVB7_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR1912168.1"
                     /translation="MSDKNIEFIESRYAAFIAGLIESSPMSQAQIARLIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
     gene            complement(43763..44866)
                     /locus_tag="ACHVB7_000052"
     CDS             complement(43763..44866)
                     /locus_tag="ACHVB7_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1912169.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAIMISQINNNMIFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSTLMRTCIEELFEYGKDSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELA
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRLNFS"
     gene            complement(45074..46987)
                     /locus_tag="ACHVB7_000053"
     CDS             complement(45074..46987)
                     /locus_tag="ACHVB7_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR1912170.1"
                     /translation="MSFIDYDILSASSSKELREKLAEKIAEGWQPFESPNIVEPSKGG
                     PNAYNQAIVRSGDLSQLPTPVSNEDEEPDYYYVVTIAGQSNSMSYGEGLPMPKTFDRP
                     HPRVKQLARRSNIKPGGFACKYNEIIPADHCLHDVQDMTKFKHPKANDSEYGCVGQGL
                     HIGKKLLPFIPANAGILLVPCGRGGSGFTVTGSEGQYYEESGAIESSLKWGLNTPLYK
                     DIVARTKAALSKNKKNVFLGVVWMQGEADLATGKYSQHNELFLKMVEGFRKELSQYDA
                     QCVFGSAANVPWICGDTTHWWKNKYPKQYEIVYGGYKGKTSQAIYFVPFMKDENDNNV
                     LTNSPTDDPDITSIGYYGSGHRKDNKTWTSESRESHFGSWQRRGIISDRLATALLAHS
                     AKSSAFVNGVTGEIIKQEQPTQPKNDNIVTTPTPRTVISLTGAGGDVATEQGWTVTGA
                     NIQSVENGKAIALTKAVSNETWTISKATDIAQALLENGGKLLCKFKLSGNPDPKKAKF
                     GFGIYYKTSKPVADDISFDGTGSLFLANFFLQTIGGKLNIMMHRTGNNRNVQIDSYGD
                     YDNNWHTLELQYEKGTGTVIPVLDGKAGSKFKLVKDNIALGDEAFVLTDITNNQTHDL
                     AIETLTISINNPT"
     gene            complement(47213..47299)
                     /locus_tag="ACHVB7_000054"
     tRNA            complement(47213..47299)
                     /locus_tag="ACHVB7_000054"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47265..47267),aa:Ser,seq:tga)
     gene            complement(47790..47865)
                     /locus_tag="ACHVB7_000055"
     tRNA            complement(47790..47865)
                     /locus_tag="ACHVB7_000055"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47830..47832),aa:Thr,seq:tgt)
     gene            complement(47870..47956)
                     /locus_tag="ACHVB7_000056"
     tRNA            complement(47870..47956)
                     /locus_tag="ACHVB7_000056"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47920..47922),aa:Tyr,seq:gta)
     gene            complement(47962..48036)
                     /locus_tag="ACHVB7_000057"
     tRNA            complement(47962..48036)
                     /locus_tag="ACHVB7_000057"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48002..48004),aa:Asn,seq:gtt)
     gene            complement(48796..49086)
                     /locus_tag="ACHVB7_000058"
     CDS             complement(48796..49086)
                     /locus_tag="ACHVB7_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HFR1912171.1"
                     /translation="MSNLTSWDWWLATYFLAVGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            complement(49232..49447)
                     /locus_tag="ACHVB7_000059"
     CDS             complement(49232..49447)
                     /locus_tag="ACHVB7_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912172.1"
                     /translation="MIVLSKREKETLHEISKWPEFPQYWKPKTRANLERLGLVANISE
                     TWCSANYQLTDKGKVLLQQLVESGVLK"
     gene            complement(49444..50766)
                     /locus_tag="ACHVB7_000060"
     CDS             complement(49444..50766)
                     /locus_tag="ACHVB7_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFR1912173.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESIPSDEKAIDVLNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTGMKLKERLAML
                     VALFKNDRSEGYTQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            complement(50763..51245)
                     /locus_tag="ACHVB7_000061"
     CDS             complement(50763..51245)
                     /locus_tag="ACHVB7_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912174.1"
                     /translation="MAVVVKADLSKIPSISGNNGYSLRCEEVKINGESAYCSYSVCQH
                     TILAFKENRLPRTSFQSCATAIKAGKCKALKMMVEEIRKGESLYFEDMTALIKEVEER
                     NKQARTLKRKRDSVTINSMVKKSTTSQTAITDVYAALLEETTKETHEQIDQHMEVKQQ
                     "
     gene            complement(51301..52077)
                     /locus_tag="ACHVB7_000062"
     CDS             complement(51301..52077)
                     /locus_tag="ACHVB7_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912175.1"
                     /translation="MKDEIQKLACDIIDKTGLEISESNRLDIIEKAVKTAMDHIATRL
                     VEIPLPGLPYLKVKLHVWGEPSCARRSALVVFISKENPLSLKVQVGAWLDGRVIYTNT
                     VFCLSNDETIEAAIQEAVLAMRSSGLMKNNYEEYLRSISGEKTLSLKADFVTPKNLLE
                     VLLNKGANDAVNVIRESEYASLCDMCKSQLDLVHIVIDAGKACDGVMAEFAWKVVRIA
                     NELPMIEQEAKSYATNHVTELLAPYRLESNQRKMISWGSW"
     gene            complement(52153..53268)
                     /locus_tag="ACHVB7_000063"
     CDS             complement(52153..53268)
                     /locus_tag="ACHVB7_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1912176.1"
                     /translation="MSELKELIAELDFEQWLDTEGIVYRRGGVSTRGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEELVLASTVELEGPVALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLTSIGLNVKVACLGEEGL
                     DPGGATPEQIIKAYYRAKPYTKQLELQSKVLGIKALS"
     gene            complement(53416..54756)
                     /locus_tag="ACHVB7_000064"
     CDS             complement(53416..54756)
                     /locus_tag="ACHVB7_000064"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFR1912177.1"
                     /translation="MTELCHTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDLVVPS
                     QFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFADVVAAFKRI
                     LAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIGSNEA
                     TGIYDYYTSASERLKAREYEASEEYVPNSITTGLPLLDRLLYQKGWAKREMVLFMGFA
                     KSGKSTAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDARLSETEMSKLVERRDEVH
                     RKLAELGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMVVVDYADLMRAS
                     YDLRDDRANIRSIYTDLRALYDKHNVAGITASQTNREGGASEVATMMHAADNIEKVRI
                     ADLVITINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT"
     gene            complement(54800..55540)
                     /locus_tag="ACHVB7_000065"
     CDS             complement(54800..55540)
                     /locus_tag="ACHVB7_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912178.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FETKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELVEIFEEK
                     LARRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILECLRTIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARRFIDPLSN"
     gene            55830..56888
                     /locus_tag="ACHVB7_000066"
     CDS             55830..56888
                     /locus_tag="ACHVB7_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912179.1"
                     /translation="MRMNRLPGYGLPELAFWPQPKYERNKWSMFCLKLRNDGTLAWYR
                     RYVDRGMPNHAVDDDYDNYPEARKAALELNKNVSFDIEKLPLSMFQKKSLRLKVDKAL
                     KAKSRLMDEEYIMLNEAIKAHANDPRVTLNELIINPDYETLRQPLFDVLSEMPYLHFV
                     FLPKFQVSLQLVAPNTWECSYNNKAKTAKICYQERIARGFGLSGAAHWGKTKATIRSM
                     LLPRANRLLQLASVKRMLDEALRNGQKVLVVGNFVFWFEDKNQVGWSVKVASESETTS
                     RGNTLWKEGTIISKNHGRIVVLPYTKENGKHVKGYTKNAPNDGNAIPRHKDEYVELPF
                     EILDGDLMFSLFGELNYE"
     gene            complement(56949..57302)
                     /locus_tag="ACHVB7_000067"
     CDS             complement(56949..57302)
                     /locus_tag="ACHVB7_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912180.1"
                     /translation="MKMGDLAKRKESDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            complement(57308..57976)
                     /locus_tag="ACHVB7_000068"
     CDS             complement(57308..57976)
                     /locus_tag="ACHVB7_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1912181.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            complement(58139..58915)
                     /locus_tag="ACHVB7_000069"
     CDS             complement(58139..58915)
                     /locus_tag="ACHVB7_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912182.1"
                     /translation="MSPSFIAEYSKVLSDFPECEAREKIRVGYMVNSDFSVLRLTEWS
                     LIGSNYDYCLTVSFVGDPDADWLQEHAMDLDEQGHSEEEIVHSVRELVGDEQEDDDGI
                     QIARFVYKRFQFRSQKGSHLGVQIKGAFVTPSKEQKGLARRVYNFLLNWHDHVVCDDH
                     QTVYGARIWAVGMLLVGRVQIYDNLNKEFIDVLVEGGIGQNGVKPWDALLLNSEAQLR
                     HWNPMAVSIDPSSQILTIISKEDRHIEVGVTTFDARSTNH"
     gene            complement(58916..59314)
                     /locus_tag="ACHVB7_000070"
     CDS             complement(58916..59314)
                     /locus_tag="ACHVB7_000070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912183.1"
                     /translation="MHTALNASRSIQTIRDCTKMIASVPLRHWNDSLQIREMNKPGML
                     KDQRFIVNLKSDVEEGKFTIKLFAEMMVLNNRLRFLVKPEKQFPDVVRCEQDAFYEIE
                     SKIAKFIKDKYRVDVKIAKLDSSTDMRLAN"
     gene            complement(59640..59891)
                     /locus_tag="ACHVB7_000071"
     CDS             complement(59640..59891)
                     /locus_tag="ACHVB7_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912184.1"
                     /translation="MLGSLMIKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQSTVDVKNETLDELRRKDASTVHHELHNKWLRD"
     gene            complement(59893..60585)
                     /locus_tag="ACHVB7_000072"
     CDS             complement(59893..60585)
                     /locus_tag="ACHVB7_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912185.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGSSIDTIREVIERWAPPNENNTDTYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRKHQKGMA"
     gene            complement(60599..60922)
                     /locus_tag="ACHVB7_000073"
     CDS             complement(60599..60922)
                     /locus_tag="ACHVB7_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912186.1"
                     /translation="MANDVGGVTHDQVERIAKIVAREVVGKLGKEIREEIVHEVHEQL
                     KNYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIISKVTSFIITALILGLAAYGV
                     KNGLQ"
     gene            complement(61020..61532)
                     /locus_tag="ACHVB7_000074"
     CDS             complement(61020..61532)
                     /locus_tag="ACHVB7_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912187.1"
                     /translation="MGVASGWVGSSAKSETGEQWMSAAGTKLGLGKPFMMSQMVGRSM
                     GCKIATAYYKGNPPDKVENWGAVGSDWPLAEKNQGTIAGASSCGLGRLVGIVLTQAHY
                     VPGLPPTGGLYIAGGRASNITVTVGGVSQVFTYNSVINGFHYYWQGTPSSAFITAMKR
                     TGVTQDLKIS"
     gene            complement(61618..64989)
                     /locus_tag="ACHVB7_000075"
     CDS             complement(61618..64989)
                     /locus_tag="ACHVB7_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1912188.1"
                     /translation="MWYREGTITFTQGSNTLVGAGTAWNVTANGVLPGMIVVAPDNKL
                     YEIKSVTNDTTLTLVENYTGETQSDIPCRIITTYEGDLTQFSARFTALLSRMSGDAKT
                     VRNWLTAVDEVTLEREDGTNVNVKPLSKIIAEHNKHVEWYENNTNAINSAGDKAKQAA
                     ASASAAAKSANLASSKATQASNSALDATASKNAANKSASSAKASETSALEAKNAAAIS
                     ASTASTKATEATSSANAALVSKEAAKLSEMNAASGAGSASTSANQARESARSAKTSAN
                     QASTYASAARASKDEASRKSAEASTSASNASSKSAESAAKAEEAKQSQTSALASAERA
                     EIAATRAERALSAISLHDASLTMKGIVKLSSETSSSSEEFAATPKAVKAAFDLAKSKY
                     TANDATTSQKGLVQLSSDTNNASESLAATTKAVKSVKDLADRKAAINSPAFTGTPTAP
                     TATQGTNNTQIATTAYVREAISGLVGSSPEALDTLNELARALGDDPDFATTMTNKLAT
                     KQPLDATLTALAGLTTAANKLPYFTGNDTAGLATITSVGRDILSKASVQAIIQYLGLK
                     ENGTSGDKIPLLSTANTWSAKQTFSNGLAGSLNGNSDTATKLKAARKIGGVPFDGSSD
                     INLPGVNSTGTQSTSGNAASATKLATPRSIGGMLFDGTANIDLPGVNKVGNQSTTGNA
                     GSATRLQTARTIGGVSFNGTANIDLPGVNKAGNQNTSGNAATATKLATARKINGVSFD
                     GSKNISITPKQLGAAKYVANLATGNTQEWTTAQFVAWLNSQGAFDATQWSTRCSWSYG
                     SNCYIGNASTGCGVIPLAGSVIEVFSTGTSNYTIRITTPSTSGGASGAVLNSEFIYTY
                     NGDNYSPTWRRNYSSQNKPTASEIGALASNGNAVSATKLKTARNIGGVSFDGTANIDL
                     PGVNKAGNQNTSGNAATATKLKTSRTIGGVSFDGSANINLPGVNTSGNQNTSGNAASA
                     TKLQTARTINGVSFDGTKNISISAAQIGALPIGGGTITGNLTVNGNGSFNDVQIRSDK
                     RNKRNLVKLDNALDRLEALTGYLYEIQLSDDSWKTSVGLIAQDAQKALSELVTEDTDV
                     ISGEKRLCLNYNGIIALLVEGFKTLRHEIKELREK"
     gene            complement(65084..69790)
                     /locus_tag="ACHVB7_000076"
     CDS             complement(65084..69790)
                     /locus_tag="ACHVB7_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1912189.1"
                     /translation="MEQFKKKKLPLLIAGSGGKKSSKSSSRTPVEADDTVNSRAMASI
                     LDLLGEGVIGGLVNGARSIFVDDLPIVNEDGSSNFSGISWDFRDGSQDQTPMSGFDFV
                     ETPKSINIQLKKSHYVTVSIDNDEADRVRVIMKFPSLRRIDQKTGDTNGTTVEYKFQI
                     SNGDSTFVDVVAEGEKNVGIKLTAKKTGAYYRSYELKLPKPGRAYSVRVVRITDDNSG
                     QYLYNDTWVDSIGEIVDTPMNYPNSALVGLKVNSEQFGGSMPSRSYLVRGLKIRVPSN
                     YNEASNTYDGVWDGSFKLLSSSNPAWILFDLLTNSRYGLGQYVSESMIDLGQLYQIGR
                     YCDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSP
                     SDPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRY
                     GERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNK
                     YRAGKRNSGRIVEFTKNSITLDAPVSLAKSGSFIRILNQEGKIVERDVLETGENITKV
                     TFSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETSGSFDITAVENNPSKYEA
                     IDNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISW
                     RRSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISTVYQVLGT
                     MTPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTF
                     THSGLGDSETWYYWVRSANKRGMLSRPNSNLGTEATTRDVLSFLANKITSSELGQALN
                     EEIDSKASQQAVDEINNHIDQSVESLEGEISALNKELTSSIDEFKTSFTERSETLENA
                     QSELRTEVSSTREKVDDALRQVENSNAALIELRNTVSKQGDSVAGVVEAANAALENAS
                     SLIAEERKVRAQADLANAKKIETMRSSVDESVAAVEEMRRTVAEVGRVSSEITTNLEA
                     ISKTNIDLALRQDEDQHRQLVNNAKIATTQKILVDDVSAMASKVEEIRAEIGDNIRAS
                     ILEEATARTDGDNALANRVTQLQSKFEGDISAAIRTEQSARASANETLSKQITQLESK
                     VNTDISTAIREEQQTRATQDEALSRQITQLQTKVNNDISAAIKSEQQARVSGDSALST
                     QINQLQAKVDDDISAAINEEREARASGDSALSREVSSLKTKTGKDIAAAVAVETKART
                     DANDALSRQISSLTTRANNLQSSIERESTSRTSQDSAIAKDLASFKTKTAKDISAAVA
                     VETQARAAADTALTSQINSLKTQTGKDIKAAVAEETKARTDADAALSTRVTNLQSQTT
                     NYINAAITSESKARTSADNALGKRIDTLKASVDGNVAVIQQQASAIADANRKVSTSYT
                     LKMETSTSGGQKYVAGIALGIDTTGLSQFLVRADRFGLVNNVNGKITTPFVIENSVAY
                     MNGAYIKDGTITNAKVGDLQSTNFVRGRAGWRFGKNGTLEINGNSGGNGRLVINGQRI
                     DVYDDNNVLRVRIGRL"
     gene            complement(69807..70400)
                     /locus_tag="ACHVB7_000077"
     CDS             complement(69807..70400)
                     /locus_tag="ACHVB7_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1912190.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAALLVVSAVMMPALAPLGLSLMMGGIAQIISPQATNESVRQADNSNSYYFD
                     GPQNTTNQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            complement(70388..71185)
                     /locus_tag="ACHVB7_000078"
     CDS             complement(70388..71185)
                     /locus_tag="ACHVB7_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1912191.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLCRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDVFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            complement(71178..71876)
                     /locus_tag="ACHVB7_000079"
     CDS             complement(71178..71876)
                     /locus_tag="ACHVB7_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1912192.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            complement(71959..72294)
                     /locus_tag="ACHVB7_000080"
     CDS             complement(71959..72294)
                     /locus_tag="ACHVB7_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1912193.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERSGVESFYWVNPRGKQIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEA"
     gene            complement(72337..76893)
                     /locus_tag="ACHVB7_000081"
     CDS             complement(72337..76893)
                     /locus_tag="ACHVB7_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR1912194.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAVGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASASVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLINKPVRVRTSGSGNGGGSGGR
                     GGYSWHIGKNNEGSMFSGLRGNIFLLGEIGDAARTVTSIMFGWQKPIIEAAAEMERMR
                     VMLRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLK
                     ALVDSVARFGGDSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGI
                     TMGELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFAD
                     RVGQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQEL
                     VINLGKVVAAMVAFKLMRAGIMGVVGAGSKMVSTFATMATAIQTPFNLGATAVTRFNR
                     AARMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMT
                     MLRSETSKVVDEIRKIPEAMTAAKRAQMADRAEKLEKQILADQRALKTGEGVNYVSNS
                     AGVITHKESKADIEARLKKNQEEYQKITGTIALGDGAVAKRLAKEAAESQIEKIRAEN
                     QIFAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVDSF
                     SSRKNATEKQIATLNDQLEKAKKDGNTEQIQKLQGSIRGYQEHLEAVAQELTQAEFER
                     DNAAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMDFE
                     GKPIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQRS
                     ANASQRAAKKEQTAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLT
                     DLANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPESDSIIS
                     AGYTPSREEKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTALIK
                     ETGTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDIT
                     NMVVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSVGE
                     ATKETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSM
                     QNLSSVTGALSAAFAMLGANASGSGNKWLSFGATVASGLVSAWAGGGFDSLTSSSAKT
                     ATSSVADGTKGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGSMNEAY
                     VPLPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNAAQRMK
                     AIALETIAQEKRPGGSLNPNTQRN"
     gene            complement(76901..77125)
                     /locus_tag="ACHVB7_000082"
     CDS             complement(76901..77125)
                     /locus_tag="ACHVB7_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912195.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARAAQADGEGVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDRADPDAKEQLMQIFGRG"
     gene            complement(77251..77568)
                     /locus_tag="ACHVB7_000083"
     CDS             complement(77251..77568)
                     /locus_tag="ACHVB7_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912196.1"
                     /translation="MTKLLDLDSILPPKKSVKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LTNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            complement(77636..78373)
                     /locus_tag="ACHVB7_000084"
     CDS             complement(77636..78373)
                     /locus_tag="ACHVB7_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFR1912197.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QQGQTVISELVQGRNITITAPLAESVLKNMVDLMPGSTLSEDDNSVTITSAQGVNLIN
                     VAKELVLTPQDTTDYVLTLPKAATAGNFTMSYQSDDVRVFSVQFNAYPDDEGVLGKMS
                     GPKPVKNVTITPESPSVKVGATVQLTANFTPDDVVDKTGVWSSDNKKATVDHTGLVRG
                     VEQGSANISFTSNSGSKKVTKLVTVNQ"
     gene            complement(78447..78830)
                     /locus_tag="ACHVB7_000085"
     CDS             complement(78447..78830)
                     /locus_tag="ACHVB7_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFR1912198.1"
                     /translation="MFIEAFANLMQKAKIGTVGTDIFCHYLPAYVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTIEKARKIIDMFPVEETESGEVYFRLVRPMAMP
                     VVYPKNDGSLIEAGIPVEFAGYLLN"
     gene            complement(78832..79305)
                     /locus_tag="ACHVB7_000086"
     CDS             complement(78832..79305)
                     /locus_tag="ACHVB7_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912199.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIEGVKPRVGPHFLERAVEISEKQFSEAILEAAKKAGFTRG"
     gene            complement(79296..79640)
                     /locus_tag="ACHVB7_000087"
     CDS             complement(79296..79640)
                     /locus_tag="ACHVB7_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912200.1"
                     /translation="MNISWQTEIAIYRFGTKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLESAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            complement(79712..80545)
                     /locus_tag="ACHVB7_000088"
     CDS             complement(79712..80545)
                     /locus_tag="ACHVB7_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912201.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITSGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNTFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDIVAAREDGILSESIGETTNMYRTGKSANVHVARSTWRLLIRYIDNRMIVRR
                     S"
     gene            complement(80545..80979)
                     /locus_tag="ACHVB7_000089"
     CDS             complement(80545..80979)
                     /locus_tag="ACHVB7_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912202.1"
                     /translation="MKPAKIRLLEPQFSDYSGMLCGVKFENGVSVSELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYGERNDLNVDQIVEPSAPDIVPMKRGTADEPAKPIQTFTRE
                     ELESIADSEGIAGLRLIGNKIGVKAKGIVEMIDGIMKAQGGE"
     gene            complement(81077..81997)
                     /locus_tag="ACHVB7_000090"
     CDS             complement(81077..81997)
                     /locus_tag="ACHVB7_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1912203.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREATLSEATFIDVNDTIAEGAATFSEHVAKLRILAGDVDVDKFLATTMSDTNNQL
                     AVQVRQKVKGLARAFRRNLILGNSETNTKAFDGIPRLMHKDQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGIPFIVND
                     FIPKNGVKVTEDSEQKTEGQEGTANIYCLHLSEENGLTGLYGGDNAGIVVENIGTVQN
                     KDAVRTRVKWYCSLANKHDKAIAALNNVKI"
     gene            complement(82023..82910)
                     /locus_tag="ACHVB7_000091"
     CDS             complement(82023..82910)
                     /locus_tag="ACHVB7_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6651 domain-containing protein"
                     /protein_id="HFR1912204.1"
                     /translation="MNLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTQEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENTRREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERYAALE
                     SENASLKSQLVEMTVGASFSNSAFLRDKVLMTPAKARVIYGSHFEVGDDGSVVGYDKP
                     AGHKDRAVLVDGEGNTLSFESAIERIVRADPEADAVMRSEAKQGAGSNSKPTHKVVQQ
                     KSKSTMDKLTSGLGKIGLK"
     gene            complement(82932..84506)
                     /locus_tag="ACHVB7_000092"
     CDS             complement(82932..84506)
                     /locus_tag="ACHVB7_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1912205.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGEHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDRGAAQESG
                     QDQPRGNGDSSTQENE"
     gene            complement(84533..85789)
                     /locus_tag="ACHVB7_000093"
     CDS             complement(84533..85789)
                     /locus_tag="ACHVB7_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFR1912206.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQVTVFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMSASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            complement(85789..86421)
                     /locus_tag="ACHVB7_000094"
     CDS             complement(85789..86421)
                     /locus_tag="ACHVB7_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1912207.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDE"
     gene            complement(86618..86884)
                     /locus_tag="ACHVB7_000095"
     CDS             complement(86618..86884)
                     /locus_tag="ACHVB7_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912208.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
     gene            complement(86894..87784)
                     /locus_tag="ACHVB7_000096"
     CDS             complement(86894..87784)
                     /locus_tag="ACHVB7_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912209.1"
                     /translation="MADIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            complement(87790..88044)
                     /locus_tag="ACHVB7_000097"
     CDS             complement(87790..88044)
                     /locus_tag="ACHVB7_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912210.1"
                     /translation="MPDLKIVELKPSKETDNNNVEVIRLLEEALQHAREGKSQSLALL
                     MINNDGSVLDCWHNGGRPYVMVGAMESLRLDFINANIERR"
     gene            complement(88037..88675)
                     /locus_tag="ACHVB7_000098"
     CDS             complement(88037..88675)
                     /locus_tag="ACHVB7_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1912211.1"
                     /translation="MSKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYA"
     gene            complement(88672..89340)
                     /locus_tag="ACHVB7_000099"
     CDS             complement(88672..89340)
                     /locus_tag="ACHVB7_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFR1912212.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            complement(89340..90020)
                     /locus_tag="ACHVB7_000100"
     CDS             complement(89340..90020)
                     /locus_tag="ACHVB7_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR1912213.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEALVWL
                     VDYYKERM"
     gene            90103..91662
                     /locus_tag="ACHVB7_000101"
     CDS             90103..91662
                     /locus_tag="ACHVB7_000101"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1912214.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQLVQQMLSYEVEAGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAKNGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQATRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            91665..91943
                     /locus_tag="ACHVB7_000102"
     CDS             91665..91943
                     /locus_tag="ACHVB7_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912215.1"
                     /translation="MSEKKTTYCQVALSDKANDKLGKFQMKLKEKNIKMSKAEVINTI
                     LETMTMAEFDKVSSAVGVSAKAREKIMRIYENSNMTKEDLEQILSKLP"
     gene            92125..92718
                     /locus_tag="ACHVB7_000103"
     CDS             92125..92718
                     /locus_tag="ACHVB7_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912216.1"
                     /translation="MKRSLLLACIYLSSFSANALSDIVSDMQKTYSMYTSEDVLKYES
                     RGLIQPVWFRFSDNAEETILNAGKERLRLQINGTAVPENVNVSSDEAMKPSGIVLEVQ
                     DGTLYCDEMATTGCQVNIRVGNEEPIVTYAKAEGNTSGKVLFIDENTSKRIVRDIQKT
                     ANSKSVDKKIYVEVPFFVVGNKEFKFGLLKTPLRVSE"
     gene            92715..93236
                     /locus_tag="ACHVB7_000104"
     CDS             92715..93236
                     /locus_tag="ACHVB7_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1912217.1"
                     /translation="MRTLDITYGIPAEVWPRDYEHAERTLLFWRSAKFPVRVTLEDGQ
                     VFCMYVYGLLSARNKLNLTPDPLSNTNRIRLPLERISTIESGIEEVDTSFTGRLTLNN
                     EQMVNQPSRRDFFKICRQAYQNGKSIRVYMADGREIEGTSDGVDACLVTLKLEDGRKI
                     LIFFDWVERILPF"
     gene            93534..94184
                     /locus_tag="ACHVB7_000105"
     CDS             93534..94184
                     /locus_tag="ACHVB7_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912218.1"
                     /translation="MANIIISKKSIIEAASIVSDELREKADLATQTYNEHYKNGTHTK
                     ADKANMQAATTKLAYFINNVVNAVEDEKLCSVFYYAIKASKQAPEVFFRDAMTNSYSL
                     EKLVYLVKSIKAGKCVYSVADMSGSRVFALIDMINDEIDTFTNGAVFDLMNEAKKACE
                     IKLDAGYTQANQLINLCERLGLVEKVKGMGSAKAGTQQYRFIKNDFYNYLADAFKA"
     gene            94234..94437
                     /locus_tag="ACHVB7_000106"
     CDS             94234..94437
                     /locus_tag="ACHVB7_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912219.1"
                     /translation="MVSYDKIRAEYRAKYRAYKLELINDLIAQRDQLNFTFSDLLNSK
                     RDCKRKREYLRLSALIGKLHNSI"
     gene            94458..94685
                     /locus_tag="ACHVB7_000107"
     CDS             94458..94685
                     /locus_tag="ACHVB7_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912220.1"
                     /translation="MVIFISGVNINNHTLVYDIAGLAGYALSSEVVDETTFKININDV
                     EHRARAGINEADVTLMLQEFLNAGFNIRLEK"
     gene            complement(95304..95786)
                     /locus_tag="ACHVB7_000108"
     CDS             complement(95304..95786)
                     /locus_tag="ACHVB7_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912221.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRDNETVELSAQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIKTQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYTAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
     gene            complement(96137..96511)
                     /locus_tag="ACHVB7_000109"
     CDS             complement(96137..96511)
                     /locus_tag="ACHVB7_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912222.1"
                     /translation="MGLDIYIETQPKNDLNNDASRKKVAYFRKFNALFGWMERNVGEV
                     KNCELLELTMNDICALKAHLMHMNESNCEEYLPTCEGFFFGSQEYDEGYWHDVGELKK
                     LVEELIKNHDFHNNRLTFCAWW"
     gene            complement(96630..97025)
                     /locus_tag="ACHVB7_000110"
     CDS             complement(96630..97025)
                     /locus_tag="ACHVB7_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912223.1"
                     /translation="MSVKRENLTVDVYYASESAEGKNVAKITVVTYNTETGTEVQAST
                     IVRKGDTSGGEYEAQYQSIFDATDPLLLKIESYFRKVNKEVFETMMNMVNTVFASSLN
                     TNTTWIGQYGLRITSGIPADTLIPESVFA"
     gene            complement(97151..97462)
                     /locus_tag="ACHVB7_000111"
     CDS             complement(97151..97462)
                     /locus_tag="ACHVB7_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912224.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWMAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
     gene            complement(97617..97946)
                     /locus_tag="ACHVB7_000112"
     CDS             complement(97617..97946)
                     /locus_tag="ACHVB7_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912225.1"
                     /translation="MFANIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMICDMGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     DFLAIFA"
     gene            complement(98085..98300)
                     /locus_tag="ACHVB7_000113"
     CDS             complement(98085..98300)
                     /locus_tag="ACHVB7_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912226.1"
                     /translation="MKMPVTVEVWGVDSLAECLDAVGPELYRKLWSFVPAEGESPKGK
                     DIWHLLSEDEKRELVGAVHSEFPDDQD"
     gene            complement(99875..100060)
                     /locus_tag="ACHVB7_000114"
     CDS             complement(99875..100060)
                     /locus_tag="ACHVB7_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912227.1"
                     /translation="MLKEDSYLLKALEMSGVEPTVKNAHDIYLQFSTELKQLINKYGV
                     NDSSVKAMIDDELDHLI"
     gene            complement(100097..100396)
                     /locus_tag="ACHVB7_000115"
     CDS             complement(100097..100396)
                     /locus_tag="ACHVB7_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912228.1"
                     /translation="MLNITQAYQVALISSSHTSEEDARRFMIASNELDWVSQIEYGCI
                     VHAGLQDDDWKDELRRYGISEGAIANIQKVLDVGFNSVHFDCGAPVVEGLECWQW"
     gene            complement(100558..102591)
                     /gene="metG"
                     /locus_tag="ACHVB7_000116"
     CDS             complement(100558..102591)
                     /gene="metG"
                     /locus_tag="ACHVB7_000116"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR1912229.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKCMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLTSELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATAAPVTGPLADEP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            102749..103849
                     /gene="apbC"
                     /locus_tag="ACHVB7_000117"
     CDS             102749..103849
                     /gene="apbC"
                     /locus_tag="ACHVB7_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR1912230.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            complement(103887..104276)
                     /locus_tag="ACHVB7_000118"
     CDS             complement(103887..104276)
                     /locus_tag="ACHVB7_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFR1912231.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKSPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
     gene            complement(104449..104973)
                     /locus_tag="ACHVB7_000119"
     CDS             complement(104449..104973)
                     /locus_tag="ACHVB7_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR1912232.1"
                     /translation="MTKFGKLEAHLLTRNYRYDVHPYRQWIDESEGVVTFPLYRFDGM
                     LAGYQTYRPGAPKQHSNPKMARYFTRAHGRQLLWGTYLPLKDGPIWITESIFKSAAVH
                     NAGGNSWALLGSTFSAGLRRQLAMLPYDFRVIGDNDAAGESLVKSFGKGFVAPDLDEL
                     QPNEVSHLIFSHSQ"
     gene            complement(104987..105841)
                     /locus_tag="ACHVB7_000120"
     CDS             complement(104987..105841)
                     /locus_tag="ACHVB7_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912233.1"
                     /translation="MMHKSVAGEFQKEVDNTTVLLDDILSILALLETGDWSEHCTKTE
                     LGGRLEREITRLIGDAQEATVTSYELIAEAWRLMDGQDPKTSDWHSKASKYLNSNRVE
                     KVDDVKPKPVDHGFRDNCECSSCQTTARICSELTDKSSLIYEVNVGGNTWGECTRAAY
                     VRAKDNGELTRVVTNHPNNELKDHQIRELVNELRDIAVTYHGTQQLREKIARAVNNSL
                     KGGQSQPKRIRITHIGENGTEIPQFSVGDIFDVHGEEFGEPHITMPSGHVYWLRAMEK
                     YGDKWEVL"
     gene            complement(105838..106059)
                     /locus_tag="ACHVB7_000121"
     CDS             complement(105838..106059)
                     /locus_tag="ACHVB7_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912234.1"
                     /translation="MNKAELFQKISALATECHAIASELDVGDERTEMFEIYSVLRNLC
                     RRCYATQVGRMTNPLLSSCDEDDSDEDDE"
     gene            complement(106052..106627)
                     /locus_tag="ACHVB7_000122"
     CDS             complement(106052..106627)
                     /locus_tag="ACHVB7_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1912235.1"
                     /translation="MTTITDKKQYPGEQYLNELITNIEFAARAPVEVVRAMAAELQKR
                     READSAEPASNHEELPLDYLQGQKDGLEWAAQLAEANHPQTGDWLYDDPLDLAKAIRK
                     GPDIPEFAGPHPVTPDGWISCSERMPEKGQNVLISVNFDSSLVEPLICSARYTGITFR
                     RGDVTIKPGNGIEQATHWMPLPEPPQESKSE"
     gene            complement(106629..107015)
                     /locus_tag="ACHVB7_000123"
     CDS             complement(106629..107015)
                     /locus_tag="ACHVB7_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912236.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AKPIGAFHIAEQQVDGTSDYLKDGEWPIDNGIIEVYAAPPVPVVPEEKPMPNPLSMYA
                     VDAVAAIAEVRGWNACRAAMLQGKGE"
     gene            complement(107026..107289)
                     /locus_tag="ACHVB7_000124"
     CDS             complement(107026..107289)
                     /locus_tag="ACHVB7_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912237.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDERILFPSSVESTAG"
     gene            complement(107291..107812)
                     /locus_tag="ACHVB7_000125"
     CDS             complement(107291..107812)
                     /locus_tag="ACHVB7_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912238.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMDVTIVVHSPGRIGSTPSVEV
                     QSIQAGFDWDAGKVMIFPAQPLTTLTPEQVADITDSVRKGQSWHAYQEYKKHKEQLEK
                     LSMELEAAKQREKDLFMENVRLKSGIAGLIHLGIRYADVEVMKIAGDAQLSTPCTDSI
                     INSIAAGIRIKGE"
     gene            complement(107809..>108101)
                     /locus_tag="ACHVB7_000126"
     CDS             complement(107809..>108101)
                     /locus_tag="ACHVB7_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HFR1912239.1"
                     /translation="KAFHILERLEALATEEEISPEKLVEFSRVILRRKNDMERLTSGA
                     PSLSVRRTLCCSFCNKSQYAVKKLIAGDAVFICDECVDVCNRIIRGEKEGSA"
BASE COUNT        27682 a        26152 c        23167 g        31100 t
ORIGIN      
        1 tctgggtaat tgcagaatgc gatgtggtca tctcactctc ctttgatgcg aatgccagcg
       61 gcgcgggaat cattccatcg ctttacttct tcacgaatta cgtcaatgca ttctttcgaa
      121 tccattaggt aatcttcatc aaaaagacgt tcctgttcgt tttctatcgc aacaatgatt
      181 gcttcaacta acttttgtgc ctgagaatca ctttctaact ctgctatgcg cttacttcca
      241 tccgagataa cgccctcgta atactcacgc tgctcgttga gttttgattt tgccaactcc
      301 agttgttttg ttagttccgc aatacggcaa acatcgttga tacgcgtttc ctctaatgcg
      361 ttgatctcat ccagtagtgc cagcacggtt tctggtccgg ccagaaattt gaaggcgttg
      421 agggcatcaa tatcaacacc gctatcttta agttcctgtt cacttaacaa gtcatcatca
      481 gctggcaaca ttaacaggcg ttccattgct ggaattgcac gttccgccac ctcacgcagt
      541 gcctggtaat taatttcgct cactggctgc ctcctgcttt tctgccttca acaccatgcg
      601 agaaccatca tccagctccc acactatctc accaccttca gccatgacca gttgccacac
      661 caattgagca gcctcattgg taacatcacg acctggatca ttgccaacgc gcatacgtcc
      721 accttcaaca tcgcgcattt ttgccagcat gatagttttt gatagcggtg aaaaaccaag
      781 ctgtagtcgt gctgaattac tcactggttg cctcctttgc gaagctgggc agcaaagtca
      841 actaaccact cagtcatttc aaccttccct accaggtctg aaccagggtg catacagcaa
      901 tcactctgcg ccgctttgaa atccttatac tcatattctt gggccaccag attttttgca
      961 gcttctatag cagcatccac cccctgcgca cgtacttcag ccagaaaagc atcggtggct
     1021 ggagtctccg ttaattcgct aacccaatta tctccgtgtt cctcggcaca catcgcatcg
     1081 aatgctcgct cagattcttt cagggctgca ttctccgccg ctaatgcgtc tctctgcgcc
     1141 cgcaacttct caatttcggc agccatgtag taaccaacca tagcgaagac ggcaaaagag
     1201 ttgtctgcct catcaggcga cactgaggcc attagcagac catcgtaata atcgtgactc
     1261 ccattggtga tcgccacctc ataggagtcg tggttctcac gcttgtgaat gagaactacg
     1321 gggttttcga ttttgttact catgtgtttt tctcttcttt gctgttacat aagcactacc
     1381 aagtgctggt ttattttcac aaataagaaa atgtagaaaa caatttattt aaggccacaa
     1441 cttatggcct ttatttattc atgccgaagg atttgtagcg tctaattgcc gtataaaatg
     1501 gggtgaggtg cttgccagta ggctccaccg gtccatgtga accccatgcg aataagagtg
     1561 tttattgcgg ctttggtaga ctccgattcg acactggctt tacgaaactc ttgaacctct
     1621 ttcgccaacc tgtatgtaat tgtgcagggg acgattgcag cccagccttt ctcatgactg
     1681 ttaatgacca tctggatgtg accatccaga ataggttgat tagacatgtt attgaaaatg
     1741 tctgcctgtt tttgaatgtg aagaaaaagc cttgccagct ccatttgctc actacgagac
     1801 aagggattat caagagggct ttttgcaaaa gcggctatac gctgtgcgtc aatgccaaac
     1861 atcagcgcac ctcaccatct ttatggctgg agttaacttc cgccattaaa tgtctgacgt
     1921 aatcgacaag agagccacct ggtggaatct ggcattcctc aactaactgg aagtagatat
     1981 ccgcagcatt acgtgtattg ctacccgcgc atattctttc ttcccgaagt gcatgaagct
     2041 cgttgatcaa acggtcacac tctccgttac gctgatccac aacagcctca agttctgcga
     2101 cgcgttcacc tggcgtctta ccttcttcgc gttggatggt aacgacgaaa tcgccaattg
     2161 cagggacgtt gtaatccagc tcaaggtagt ttttggcacc gctgcggacg aactcacccg
     2221 cgaacatggt ggcgaacatc tcagaggcca ttgtgctgtt gaaaagtgac tccagatcta
     2281 acggagtccc ggcagccaga gcctcttttg ctgtatccat tgtatccatg aaccgatcaa
     2341 actcattagc gcgcttttcc aggtctttcc attgctcgct ccaacgtttg gcaatgaagt
     2401 ctatgaatgt tttcgcggat tcatcaaaat ccccctcaaa cttaacgatg cctttatcaa
     2461 taatgatctg gcctaccgca tcctctgcat aacctttcaa tgtaaacttg aaaggaaaat
     2521 cagaatggcc tgtgacgtta aaatgctcta actggaggtt gttcatatgt ttttctctta
     2581 tcgtttagtt attacattct tacacaaatt aagtaagtat ttacctatca tttaacgcgt
     2641 ttgaaaacgt atacgctgac tgtgatccct gtgtcttcaa actcgttggt aaacgacttc
     2701 cctctggcat aaacgtaatt atccatcgtc atccagtcca gtattggcgc agtgcccggc
     2761 aatactgcta caagacgccc gccgactttc agatgcccca gcgcagccag cgtgtgctct
     2821 ctatgacgac caagcgagta cggcgggttc ataacgattt tgtcgaactg ataacctgcg
     2881 ttgtcctcag accactttat gaagtcgcag caaatcgtgt tcgtataccc ttttccacgc
     2941 aggatatcag cgaagagagg tgcgatttct atgcaggtaa catcttccag atcggcgtta
     3001 atataggcca gaagatcccc acgtccggct tgaggctcca acagcttctc accaggcttc
     3061 aattcagtgg ctctggcaac gtactcagca atcaagcgtg gggtagggta gaactggtgt
     3121 gattttgtat ccggtattaa accggtagcc acaatcgtat ttagcgtatg gccgatctca
     3181 tacgggaact gccagtgctt tttctcctgc acgccgccaa tgaagctcag tgtgcgctca
     3241 agctcttcca cctgcgactt ctggagagct gaatcagaga agtaccatac gcctttgtct
     3301 ttgctcaatc gcccgtcacg aagcgcagtg cgaaccggca cggagatcgt cttctggatt
     3361 aacccgaact gctttggtgc ccgtgttttt ggcgcagttc ggcatggcgc ggggattgca
     3421 gcaggcatac tgtaagccag cacctcattc aacttccagg ccacgtcagg atgtatttca
     3481 aagtgaacgt tgccgttctt gaacatcttc acgcgcatca gatttccatc gacgttcatc
     3541 cagtcaccgg tctggcagtc gtttgcccga tacgcagctg atagcacctc agcagtgcgg
     3601 ttgatggtaa taaattcttt gtgcgcgaag aaatgaagca agacacgcag atcgtcgatg
     3661 tagtcctctt tgcgatagtt cacgctaacg ctgtcacgcc agaaatcaga aatgcagtca
     3721 gcgattatca gacgctcgct gaaaccgttt gtcttatttg tcttgtgcgc aggactcagc
     3781 gccttaaaca agccatacac gcgctcagag agatatttgt gcctgtcatt cagcaaatta
     3841 agcatcgttg gtatgaccgt ttctgctttg aactccggta caccaacgaa ctctttaacc
     3901 ttcatctgat agccggttct gtcagttttg atgacttcct gcttgccctc gataaatagc
     3961 tcacgccact cgtcgcgacg ggaagctggc atgatcagca gaacgttagt catatccgta
     4021 accttcctcc agtactcggc ccaaatattc tgtttcaccc attccagatc gacgttatcc
     4081 agccagactc gattcaaacg tgagcgatct tcatctggtc ggtggtttag tcgaagcagg
     4141 cgattaatca tgttgtggcg ttcatcgcca taaacgaagt cgtgtacctg atacatgaag
     4201 gagatctctt tctcgcactc ggccacaatt tcgtggatga cgttcatttc ctgtcgatag
     4261 tcgatagcag tgtttgagct ggcatcgccc atgatggaaa gggctgtatt cataattgca
     4321 ccattaagaa atgatttgtt taatgatgca attatttcaa aaataaaaag gtgctaaata
     4381 gaaattaaca ggcaatactt aaaataaagg cattgactaa tcatacttgg atgtatttat
     4441 aaccaggact ttatttgtat atagtttttt tacattccat gtaacattac ttgaatacca
     4501 ttcaccatta tatatttggt ttcccgttgc gcctgtcatt gttacgtata caggtctgtc
     4561 gtaaggtgtt cgtctatagt tatatactcc aaccattgca ggcattatcg cacacgggta
     4621 tcccatattt atggtaaatt gaggatttgt aatagtgata atctctgcat caaggggcat
     4681 catctcaccg tgatatacca ttttacctgc gctattatac atggcaatac catatcctga
     4741 atgtggtaac accatatcag aaaaagcata aactgttatc gttccggggt ttcctccaac
     4801 ctgattcagt ctgagaacat tatacccatc tctaaattca tgaacgaaca tcatattcgc
     4861 cttattactg ctttttataa agaaaaaaca tgatttgttc gacggtattg aagtcttaaa
     4921 aacagaacct ttagacgtgg acattgtccc cctattaatc aaattttgcg gtgtaaattc
     4981 tggactcatc cacacattac catttgactg agtaattgac atcccgaaca ttattgttat
     5041 ccccaatatg tatatatata agatccaagt cccttgtata aatttgacca acttactgta
     5101 ttgccgttaa tagtgatagt tggtgtaggt atatttacat ggttatcgtt atttaaaggc
     5161 atcaatgagc acacagcatg taatgatttg ccaggtggtg gatttgggta agtcttagag
     5221 cctgacgtgg ttgtaaatcg atcaagaaaa aaaataggag taaggaagcc ggtaacgtta
     5281 actcccttat tattgtagat ccctgtaccg tatgccattt ttcatccttg tttttatcaa
     5341 cataatatgg ccgatcatgc ggccatatca gatcaattag agctttgaaa ggctttgtgt
     5401 ggctgtgttg cgaggttatg tcgatggctt aactcacgca tcatgtcttc aaggcgactc
     5461 tttgtgtcgt cgagttggtc agccattgct cctaaaagct gacgaactac catcggatcg
     5521 tcgctgttta gtgatggcat tttataaccc gcctgagaag acatcagatt gaatgctgac
     5581 atcaacatcg ttagagagga tttaagcccg gcaatttcac gatctttgct ggcaataatc
     5641 gtctcacttt tgttactgtc atgagttcct tgagtctcga tcaccatatc caaagtctct
     5701 tgcaacttat cagctctctg tttttcagca agataatgaa caccgaaatg atgtgctaaa
     5761 gctacaacct gagttggctc ttcaaaggtg gactcaaaat taagtgctgt aaccactctc
     5821 tcaaaaatgg aaacgtcttc tattcctcga agaatggcta acattttgac aagttcatta
     5881 gcatccattt cttcgaatag ggcgttttgt tcatcagcta ttgctttgca tttctcgcac
     5941 atatgttttc cttaatgctt aaacaagttg ttttcttatt ggctttattg tgatgtggca
     6001 taaaagggga gcaagctaaa cgtaaaggtg caaactctct atttcggtca acgaaacaca
     6061 atggccattt gacccttgtc attaacacgt tgctttacgg actagcggat aagcgaagta
     6121 gatcgccaga ccaatgagga cgccatcaga aatgacagac atcatctttc ccgtaaaatc
     6181 caccaacacc gccatcacca gaagagcaat gacggtcaca agccggaatc tctcaagcat
     6241 tagagataag catccagtga caactgaagc gcctgggcga tctttttcag cgcctgctct
     6301 tctggttcat caatgccgtc ttggtcggca atatcaaggc acagacaaag tacatctact
     6361 gcatcatcag tcccggccac atccgccagc tcacgtaaag cctgggcatt agcacggcgc
     6421 ggtgaggctt cgtattgagc gcggatattg gcgctcatct gggctatctc accagcaaaa
     6481 ggagaaaatg caggcagagc tgcaattgtt ttctcaagaa tggaaatttc ttttgcatcg
     6541 catgtaccgt tggcatagga gatcatgtac ccaccccaca cggtggcttc aaccgcatca
     6601 cggttctcca tttttttgac ttcgataact gctttacgag ttttcttttt gaagaaacct
     6661 aacatgtgtt ttccttttgt tattggtcaa aacaagttgt tttctagtta aataaattgc
     6721 ttacgcaaag acggtcaatg catatgcact aacaaccgtc agaaataacc aagtaccgaa
     6781 ccgactggaa aaataaaaat cccaactaca cgagccaagg tcatgccagc ctgaaactgg
     6841 agatcgccag agcaaacaag ttttataatg ttcgataccc aaccggcggc catgagggca
     6901 agaattgcca gccaaatttt tccaaagtga tttgaaaacc agttcataca accgccttag
     6961 tcacagcatg atcctgagta actggatgaa cttgaggtat cgcatccacc gtcatcccat
     7021 ccagagtgac aggtcgctct actggagtgg tggtaattgt cgtcgacgga ggtgtggtga
     7081 tggatgaatc cggcatcaaa aggctctgac cggctggaac ggctaccgtc gccagtgaaa
     7141 ctataatgag aacgtaggcc attggtttca gcctctttct ttgaaaattt tccattcgaa
     7201 ctgtccttgt tgtttttctc cttcactaaa ggccgtgcat ttttcgcttc gcgtgaagaa
     7261 ccagaggcaa caatcgccca cacgttcgca acaactgacg cttgattatc taaacgttca
     7321 ttcagcaacc gaacggtagt ctctagttca tcaaggcgtg acataacacg accgctaaat
     7381 aattcggcca aaatttgacg taaagaacga ggacgtttag ttacagaagt gaaataggtt
     7441 tgacgtgcca tgtggactcc atccagtgtc ataaagagtt gcggctggca atcgccagcc
     7501 gcctttctcg ttccatcctg gaactgtgtc ttaccgacac attgtcatcc tgacgccgat
     7561 aagatacatg atttataatg ataggtaaac acttacttac tgtttgttat aaacaaaacc
     7621 aatatctttc ttgtgaggca tatctgctgc tgaaaaagct gcgatcttcg cgagtcgatc
     7681 acatatttca ttttcacgat gcccggcgtg acctttaacc cacttccagc gaacattatg
     7741 tcgacttgcg gcctcatcca gacgcttcca cagatcaaca ttctttaccg gttttttgtc
     7801 agaagtcatc catccattgc gtttccacca tttcatccac tgtgtcatgc cgtttttcag
     7861 atactggcta tcagagtgca aaataacgtt gcatggatat ttcaaacgct ccagcccgat
     7921 aagtgccccc atcatctcca tgcggttatt ggtggtgcta tgaaaaccat ctgagaactc
     7981 gcgttcctcg ccacgatact ggagaacgat accgtaaccg cccgggccac ccggattttt
     8041 aaggcaagag ccatcactaa agattttcac ggttttaagc tggggattga actctacgac
     8101 aggcgttgtg ggattggtgc ggggagaatt tttgtttttg tcttttttgc gagtttttgc
     8161 ttgcggtcgg gctggtgtct tcgttgtcat ataaactcct gaatcaagcg ccgcgccgat
     8221 ttttcctcgc gcgtgcgcac acgcgtgcgt gttaataatt attaaaataa acaaattact
     8281 tcccagaaca ggtttttata aacctgaact gaacgaacga agtgagtgaa gttcacctcg
     8341 aacgaagtga gaggttgtct tttcaggtaa tactctccca gggaggtgag tataaaattc
     8401 ctcaccaacc tggtcgtttc ataacctgaa aagctatggt ctaagtctac tgccagctta
     8461 gacttgggaa gttatggatg acagcacccc agaaccgaga tcttcccaca ctttatgaag
     8521 gggagtactg gattcaacct ctcgaaacac cccagactcg acaatcataa agtgaccctt
     8581 gtctccgctc actttggttc ccccttcccc gacacctaaa cggcaccggt tctacgctgg
     8641 tagtgagctt ttttaaacct gacgccagtg acgcttaccc ccacccatca ggtcgagtct
     8701 ccagtctacg actggaaact atcagatctt agcacttaca attcatctta tgaatagttt
     8761 gtacttatct atcatttcga ttgattattt tctcgaccat gtagttgaac attcaaggca
     8821 aacacctcgt tgatcaactc acctaaaagc tgttcaacaa gttcgcgatg ctccccaaga
     8881 tgcaagcact taattgccca ctcgtacaag ttgaaagcct gctcacgatc tttcatcatt
     8941 tcacgagcct gggccagaaa atcgctgtct acgagcgcaa cgacattagt cgggtatgac
     9001 atgttggttt ccttaaagtg gttcataaaa tcgattttaa agcgtctggg aagggggtct
     9061 agtggattct gtagtgatgt tcaggtctgg agtctctgat acaaaacagc ctgcttccgt
     9121 atattaataa ttaatatgta gttatttata tatatacaga agcaggctaa ttacagacgc
     9181 cagaacaaac taagcagcct tctttgatgg tctttttttc ctgatcacat tcgcaggatc
     9241 ataccctccc agacttttca tgacgtccaa agggatcttg cttatagagt gcccagcgtc
     9301 ctgacagaat cctcgaaaaa caacgagcat actgccaccg gggttgatgt ttacttctac
     9361 ctgacccaaa ttgacgtcag gctcgacaaa cgctacgcgg ccaccagaca gaataatcgt
     9421 ctcattcgca tactctgttg cacgctcata ccactttgta tcgagggatt gaggaattag
     9481 catcactgta gtaactccgc gagcctgctc acgtatagca gcatcaatcc agggggagat
     9541 tttggagtag ggtggattga ggaaggcaat tgtacctggc tgtccccatt cagatttcaa
     9601 agcatcacgc tcaatgccta tgaaattagg cagctttgcg ttgtccttgt tgcaggcaac
     9661 gtcaacatca aatgtcactc caagataacg ttgaatggca gaaaacagcc aatcaggtgt
     9721 tctccagagg tcacgaagag agttgtcgcg ctttcgctgt ttgatttttt cggctgcaat
     9781 catcttcttt ctcgataggt aagtaaatac ctatttttgc atttgatttt atgtatggca
     9841 atcaaataaa acgcgccaga aatgcgaaca agaagctctc tggcgcgttt ttttggtgct
     9901 gggtatgggt tatcttaccc agcactataa agctctctat gggctttatt ttcagtaggg
     9961 gaacgtcatc caatagtgta ctccgcgaca aacttcttaa cgatcggtga ttcttcgttc
    10021 aatgttagat ttccaccgtc tctaacggca attcctgtag ctggaaatac agccatcatt
    10081 tgcccagcct gagttgacgc agtgttaagc ggatacggtt tttctggatt gctcattaaa
    10141 gcaatcttaa tactattact tgtagccttc ttttctgtaa gaaggtgtct catggaaatt
    10201 accgtgtaaa cactaatatc tggacctccg ctaaaccaat taagaaggtt taatatctta
    10261 tccttcgctt tgacaggcgc gttttcgtaa gcgtctaaga aaatatcttt ttctaagcct
    10321 gctttattga aaaacgaatc atctttatca cttaaagtaa aactaggacc tcttgcagca
    10381 cgagtttttt tatttgtatt gctatcattt gactgttgaa cttttaattc atcattaacc
    10441 tcaacacaat cttcaacctc aatgatcccc aactgttctg gctcattttc ggcagcttca
    10501 ccactttcag cgttaattac gtttataggc tcatgcgcta actccaactc atttaaagca
    10561 gagctgaaat cacctgcttt ttcttcattg aacagttctt cggcaacatc atcagaactc
    10621 atggctacgc cagcatcgag gccgagaagc agatcgtcaa tatcatcagt attttcaacc
    10681 tgtgcagctt tttttactgg ttcatcaagt gattgcaaca tggctgtcag ctcatccaga
    10741 tcatcatttg tcattacatt ttcaaccgaa ctcatgtaag tctccttctg tttagttgca
    10801 tttgcgtttc gatggctaca ttttgactaa acctatcagg cggaaaagta ttaaatacag
    10861 gcaggaaatg attgaaaggc gactgttctt taaaagaaag cctgcttcaa actctcttta
    10921 aagcaggctt ttgttttaca caatttcaga gggtaattta aaaaatccgt atttattacg
    10981 tgccttaaag aagcattgca tcattaagtt tgtgtcatat aaggcactat gtgctttggc
    11041 atgatcgtat ataaagccaa gagagaaggc caattcttca aggcgaggac gtttaccgtc
    11101 ttctgtggcc cacaatcccg ataacatagt atctataacg ggaacgtctg gcagtcttac
    11161 tccataccct gaaaactcgt gtctaataaa tggtatatca aacgcttcgc cgttatgagc
    11221 aacccacgca ctacatgcct ccatataagc gccaatttcg ctagcatgat ttgacaacaa
    11281 aggttcagct gcgagctgtt ctaatgaaat gccatgaacg gcttgagctt tcggatctat
    11341 gtttctgcga gggttaaaac gcatctccag actatcaata tgtctctgtg tctccagttc
    11401 atagcgggta attgcgattt caataatttt atgaccagca aggaaatcca gtcccgtaga
    11461 ctcgatatcg atgcctgcaa caatcgttgt catcagaact ccttacaact tcttagcgcc
    11521 cttaagtagt gcgctacgta caaattgagc cgcttttttc aaagtttctt cactggtttc
    11581 acagacaatc ggatctttcc acttacctga cgcagtgttg agaatattga tttggttgct
    11641 atccagacaa acagagatat aaagcaccgt gccaccagcc agcatcaaat gcataggaaa
    11701 cagaggcttt ttactgtctt cactgaactg agacatggcg atgttaatgg cttcacctac
    11761 ttgtgcgcca acaagcccct gcgcagtttc gaatatggca cgaatagcca gacgagcctc
    11821 tcgatcgctc ataagcgacc gggaatgttc gtccgctaca cgaatcaaag cctctgttgt
    11881 tttccgatcc agttcatctt caagcaacat ttcactaaac attttttatc cttaacccat
    11941 tttgagagtt ttattgtcgc aatgtcgaag aggcgaacaa tttctaatga acggtcactc
    12001 gaccacagaa ccggttttct atcttttcta cagcgttatc tagcattctc atcactgatc
    12061 tggctcttgc ttcaaccgtg cgatatcggt tggaaacgac gaaggtttgc agatcccccc
    12121 tttgcggatg caggtgaatt tttgtcagtt ccccgcacat caacgcatca atacgagcga
    12181 catacagacg gtctaatgat gctcgttgtg atcgcaagta atctcgctct gagagagcga
    12241 ctcccgggcg taggccagca atggcgttaa agtttgaaat agcagttttg tggcaaaact
    12301 tttcgatatc gagggctaac tcaatacatc gatcttcatt ggtatggccg accaaatcca
    12361 acgtgtaggc cataacatca gcaggtgtgc gatcgataac aaagccttca acaccacgcg
    12421 taagtagctc tatatgcttt gcgatttcca tttgaatctg aagccgctca tatagaggca
    12481 tagactcacc tactcgaaca ccgagtcggc tcatcaaact tccgacacca gcatccacgt
    12541 aagggattcc gtaatgtttg tcgatatatt ttgccagggt tgttttccca ctgccctgag
    12601 caccagtgat cccaatccgg taatccatta cgaccttctg tatacgatct gcgaaaagcc
    12661 aggctcttcc tcgctggctc gttgggtaaa aactgtttca acaggcacaa agcctaaatt
    12721 acgcatcatg cttgccggga agaatgcatc ggcgtttggc acatcaacgc cgatgtggga
    12781 gagccaaatc tcttcaacat gcggcatgaa tagcgagtaa atttgttccc cgccaatcac
    12841 ccacacaggc tctggtaatc gcaacacatc atccacacca gcagggtaga atccatttgg
    12901 tacaaagcca tgagagcgcg tcaaaacgag gttatgacgc tctggaagag ggcgtttgag
    12961 actttccatc gttttacgcc ccataacgac agtggcgttt ttggtgagtc tcttaaatag
    13021 cttcagatcg gtagaacaac gccagggcag ttcgttcgct atgccgatct catagttacg
    13081 gccaacagca gctatcatct tcatttgctc acctcataga tgaccggtct atgatgagag
    13141 tcagctaaag cggcacggag tcgaggatcg tgcaccagcg cagcgataag cagatcgcct
    13201 ttatgctccg ctaatgtgcg cttaatatgc ttctcataac tggtgccatt cggtacgaga
    13261 tgaagccagt cgtaatcaac gccaaaatct ttcagccatc gtttagtggg cgcttcaaga
    13321 gtttcagagc ggctactgat aagcaccact tcagcccctg agcgagcaaa accacgcaac
    13381 atacggctgg tggggaaaat gagttcatca ccggcaatga gctggcctgc atccgtgtca
    13441 ggtacagatt tacgatggct ggtatttgcc agcacacctt caatttcgca taagacatac
    13501 atacctcttg ccatatcaca ccgccactgg aaccttgatc cacggtagag ggtcatagcc
    13561 aagaatcaga atatcgtccc atttgaaatc atccagctca gaccattcat gagggaaaat
    13621 aacgaccgga tctgaatgct ctggagcctg tcgtttcatt agctcacaca caccttccat
    13681 gtggttgttg tacaaatgca cgtcaaagcc aaaatgcaca aatgcaccgg ccatgtgacc
    13741 cgtaatcttc gcgagaaaat gagtgagaat gccatagcca gcaatattga atggcatacc
    13801 aacaaaagtg tctacgctac gctgtacgag gcaggagttg aggatgcgtt tggggattcc
    13861 aagttcatcc agttcagttt cagtaacact gccgtccatc tccagatcgt atagcatctt
    13921 ggtgtagata gactcataac cgaagcgact gtgttgaaga ccaatgtcag ttgccatcgt
    13981 taaacgggtt tcaaaatcaa gctcacgact ccacaaagag aagacaaagt ggcaaggtgg
    14041 cagtttcata tcctcaagct cgcctacgtt ccatgcgtta agcatgatgc gacgatcggt
    14101 agggttcgtg cgcagtgtgt cgacaatacg ctgtagctga tcgatttcac gggacagcac
    14161 tacgcgatct tcactgatac ccaggtatcc ctcgactttg tacccgcgtt cacggaaagt
    14221 agcgatcttg ctcagatatt cactatggct cacgatgcgg gtatcttccc atcgacgcca
    14281 ctgcttaccg taaaccggcc ccaaatcgcc attctcatcg gcccatgcat cccaaatctt
    14341 aacgccgtta tccttaagaa attggatgtt gcccgtgcct ttcagatacc actcaagttc
    14401 gacaagcagt ggcttaagat ttaccgcctt tccagaaata agcggcacag tcccaccagt
    14461 aagcatgtag taagagggaa cgtagcaggt gcttaaagta ccagtgcctg tgcgatcatc
    14521 agtctgcacg ccgcttttaa gcaccgtctc aataactttt gcatacgatg cgtttgttac
    14581 ctgaccgttc gtatgctcac gattcaaaag gagtgacata aaacctcacg aagataagta
    14641 atcaattatt tatcacgtga gtttacattc attgataaaa caagaccaga tagataaaaa
    14701 atggtggcca taggccacca aaaagaatag tcgttccaga gttttatacg caaatcaaat
    14761 atacattagt agataatcac ctacaattat tttttgatca ttccgtacat tgctgaggct
    14821 ttagcttgct caacaaagga ggataaatcg acatcactat aggttggaga cttcagaatc
    14881 tttccatcgg aaattcgaaa accgatcatc ttatcggtgc catcagcatg gcgaaatccc
    14941 aggtctgaag agtcgtattt gcagttggcc acagcctggc gacgttcctc tacatcagca
    15001 ggccataatt ttgtcatgtt agaccgatga atttcgccaa ccagctcgac tacattgata
    15061 ccgaggaaat cagccagtcg ataggtcatc atgcacgcca catagatttt gttcatgaca
    15121 cggcgcagtt cctgaatcag ctcagaatcc ttaacagtct tgttctcaag tttatctgcc
    15181 agctcttcga gcatacatga cgcctcacga gcttcctgaa atggcattgc catatcatcg
    15241 aaaacagtgt taccgggcac aaaaattgtc tgcataaagc gatcaatgct ctgttcctgc
    15301 gtgtaatagg tcatgccggt agaaatacca cctttgatgg cgaccatcgt accaacacca
    15361 acgtataaaa agtcggccat tgcatccagc agttgctcaa tatccccatt cattgcagct
    15421 ggaatacctt ccactacagc ttcttcatga atcaggcttg cacggagacg aagcagctct
    15481 ggtgtcggca taacgcgtct cggatgctgg aagagttcat gaaactgatc gaccatcttg
    15541 taaatacttt cagtagctac accatgacct tcatgcaact catatggttc tggcttaaat
    15601 ccaatgagac gatcggtggc aagtttcagg tggtcagtca gtttggtaaa gttcatgcta
    15661 gttttgttcc ttcttaattc ttgtgatcca ttgtggccca gcgttgccag gttacataat
    15721 gctttgtatc ggttcacagg tctgcaaact ggcttagaga agccttgtcc acggtagaat
    15781 cgatctgacc taccagatat gtgctttgct ctgcctcttg tggtgcaatt tgcagtgtgt
    15841 cggttaacaa ccacttgttc atccagacca gcggatcggt ggtaacctct ggaaataaag
    15901 ccttcaggcc aagacgattc atagccagat tggtgcggta ttttacatag gtcttcaaaa
    15961 tatccgcgtt taaaccaatc atcgaaccgt ctttgaagag atactcggcc cagttcattt
    16021 cttgttcggc gactgatttc atggtgtcgt aaatgacgtt ttcttcatca gcagcaatct
    16081 ctttccacaa taaaccttcg cgaccggtac gcatgaagcg gatcatgcgt tcagttcctt
    16141 cgcaatgcag agcttcatca cgggcaataa agcgcataat tttggtgtta ccctcaagca
    16201 acttccgttc gccaaaggca aacgtacatg caaaacttac atagaaacgg atcgcctcaa
    16261 gcgcattaat ggacaccaac gtgcggaaaa gctgacggtg caaaggatat ggctcccagc
    16321 catattcatt tacgtacaga cgctcaaatt ccttctcgcc taacgactgg cgctcgcagg
    16381 tcatcccata aagcctgtca tattcagcag agatactgat cgctcggttg atgatttctt
    16441 catccgtaac aataccgtca aaaacaatgc tcggatcgtc caccatgcca cgaataatgt
    16501 gggtgtagct gcggctatgg atagtctcag agaaagacca tgtttcaacc cacgtttcca
    16561 gttcaggaat agagataagc ggcagcagcg ttgcatttgg gctacgtccc tgaactgaat
    16621 cgagtaacgt ttgataacgg agattgctca gaaaaatgtg acgttcgtgg tcccgcagct
    16681 tcgtgttgaa gtcgatacga tcgctcgaaa tatcaacttc ttccggccgc cagaagaaag
    16741 acaattgacg ttcaatcaac ttttcaaaat cacggtattt ttgctgatcg taacgtgcca
    16801 cgttgacaga ttgtccgagg aacataggct ctttggttgc atcattagca cccaaacgga
    16861 acgttgaata tgacatgtgt ttttcctttt gctttttgat gtgtttaaac aagttgttat
    16921 cctatttgtt taaacaaggc ttacaaatca aagagaaggt ggggatacac cccaccatat
    16981 gtcagatttt gcaggctccg tcgcactcat cttctggctc gacggctaca gcttgcgtca
    17041 gtggattctg cggttcgtcg tcatcacgct ttcctgcccc atctctggtg ttgtggtagt
    17101 aaagcgtttt aactcccatc ttgtaggcga gaagcaaatc tgacagtagc gtcatcattg
    17161 ggactttatc gccttcaaag cgagaagggt catagttggt attggccgaa atagcctggt
    17221 caaagaactt ctggatgatc gccacctttg tcagatagcc gcggttgtcc ggcatatccc
    17281 acaggtattc atactgttcc ttcagttttt caatctcagg cacgaccatc ttaacgatgc
    17341 cgtccttaga agatttcacc gacaccggtc cacgaggcgg ttcgatgccg tttgtggaat
    17401 tagtgatctg gctggaagtc tcgcatggca tttgtgccgt cagtgtggag ttgcgaagac
    17461 cgtattttgc aatacgttca cgcagttctt cccacggcat ctttaactca aagttggttt
    17521 cgccactttc gtctaatgaa gaacggtaat ggtcgatcgg caactgacca attgcgtact
    17581 tggtctgctc aaaccagtca cactcacctt ttgcttcagc aagttggcag ctggcatcaa
    17641 gaaggtaata ctggatagct tcgaacgttt catgcaccag tttattacca gcggtatcag
    17701 aataacgaat gccattcttt gccaggtaat aagcaaagtt ggttacaccg atccccaggc
    17761 tacgacgagc tttggcaggc acttcggctg cctccatcgg atagtcttga taatccagta
    17821 acgaatccag tgcagcaacg gcatagaacg ccacctcttt aagagactcc agtgaacgga
    17881 tggctccgag gttaaaagcg gataaagtgc atagcgcgat ctcaccgttc gggtcgtcgg
    17941 taaatgccag tggacgggta ggtagggtga tctccataca caggtttgac tggcggacag
    18001 gtgcaagagc aggaatgaaa gcgccatgct cattaatatg atcgacgttc gcaatataaa
    18061 ttcggcccgt ggacgctcgt tcctgcatca gagatgagaa cagctcaatg gcaggtactg
    18121 acttcttgcg aattgaatca tcggcttcgt atttatggta cagcttttca aacagctcct
    18181 ggtctgtgaa gaaggcttcg tacatatcag gcacatcatg cgggctgaac agagtgatgt
    18241 tctcgcttct gacgagtcga cggtacatta gacggttact catgacgccg taatcaagat
    18301 ggcgaacgcg gttttcatca ataccacggt tatttttcac caccagcaga ctttcaactt
    18361 caagatgcca gatcgggtaa aacgctgttg ctgcgccacc acgaacacca ccttgcgaac
    18421 aagatttaac agccgtctgg aaatgcttca ggaatggaat aactccggta tgggtggctt
    18481 caccattgcg gatctcgctg cccagcgcac ggatacggcc aaaaccaata ccaattccag
    18541 cacgacgtga aacgtagtcg ataattgcgg ctgaagctgc actaatccct ttcagactat
    18601 cgccagactc aatcagcaca caactggaga actgacgggt tggagtacgg acgccggcca
    18661 tgattggtgt gggcagagac agtttgaacg tacttgtaac gtcatagaaa cctttgacca
    18721 tctccatacg tgttttgcca gcgcagtcgt cttcccaatt ctggaacaga cacatgccta
    18781 ccagcatata gatatgttgc ggcccctcat gaatctcacc agtaacacgg ttttgaacga
    18841 ggtatttgct ttgcaactgc accgtagctg catagccaaa aagatcgtca cgtttcggct
    18901 taatgtaatt accgagttct tcgatctctt caaacgaata atgtgtcagc aaatccttgt
    18961 cataaacgcc cttgttaacg ttcttgacaa tgtgttggta gaagttcgga tagtcgtaac
    19021 gaccgaaggc atctttgcgg attttaaaaa tgttcaggcg ggcagccacc tgtgagtaat
    19081 ttggtgcttc tggtgagatc aaatcagcgg cagatttaac caaagcctca tgcaactggc
    19141 tggtggtcat cccatcaaaa atgctggccg cagctcccat tgctacagcg gatgcgctta
    19201 caccacgaat gccttctacg ccatacatta cgacgcggtt gtatttttct tcggataacg
    19261 gctctgtttg gccgttacgt tttacgatgc ttatcatgta tcaccaaata aagagggcca
    19321 ctaaatatag tggcccatgt taatagataa gcacttacct atcaatgaaa tcaaatgaga
    19381 gacttaagaa catcgcgcac ctggcggaac tgatctgttt gcataccggt atggatggca
    19441 gccaccgcat cagccaaatg ttcgtttttg ttcacgagaa catcctgtcc agattgctta
    19501 cgacgcagcc acggtgcttt agagtgtttt ttcgtagccc actggataat ctcttctttc
    19561 gatgtagtaa gtttattgcc gacaaaatgc ttgatttcgt ttggagtaac ctggatcaat
    19621 ggcttatcca cacacgcaag tacaccgata caaataccgt aagacgtctg cgaacgagag
    19681 ttttggctcc caaccggtag ctcacaaaaa accatatttg ccttatcaat gattggcttc
    19741 gcaacacgcc atatttcact ggctcggcgc agatcgtcac tgttcacacg aacggtcttt
    19801 ttgttactcc ccgctttagt ctcaacaaga gtaaggtcgt gaatctcaag ttcgtccgtt
    19861 tcaaggtcta aagtgcccac agcaagccca aagttgctca ttgagggatc aacaccaact
    19921 acgctgattt ttttactcat atagtctcct taccatgttc cccatatagg gttttccaat
    19981 aatctcgcct ccaacgaatt ggttactatt gccgtttgca acgttggttg gggagagttt
    20041 gcaaagtctg aatactcatt ttcctgcgtg acacgtttat ctacggcatg tttaacgcca
    20101 cttgctatga aatgacgcat gatgcccaac agcctccgac tgtctgcact atcgtttgct
    20161 aaaacatgcg ggagctgaag ttcgatttct accatttctc catacttacc aacaaactgt
    20221 agtttgtccg cactgtactt tttatccctc ctttggataa ggaaaccagt aaaagcatca
    20281 cctggcgatg ccaaactatc cacccgctta ttggcaagtc gaataaggtt ggtgaaagcc
    20341 atacagtgat gcgcacccat tcccccaaag aacccttcgc tattaatgtt aagatcttca
    20401 ttttcatggg atgcaataaa cccaggcccg acgttgggaa gatatccccc ctcaacaaaa
    20461 ggattaatca ggatcggcgc aatacatcca ggtggtagtg atttcaaact ttgcatcaat
    20521 ggcgtctcgg atattagacc aagcaacgcg gcaaattgta ctccgtcaat attgttgctg
    20581 ccccacagaa tacgtggagc cgcaccattg gaagtatgcc cattgatcag ttttatcgac
    20641 tgcatcactg caaatacctg caaaaactgc gagcggctta atttaaacga catagctgcg
    20701 cccctcttta acttccagcg taatagtttc ccggaaccat gacttcatct ctttatggga
    20761 gataatcagc acagtacctc gctcgcgagc tttggactcc agaataccca tgaggcgttc
    20821 aagaccggct gtatcgagtg catcgtcaat ttcgtcgccg ataaacaaat cgatgttttt
    20881 actcgcccgg ttactaacca gatcctgcaa tgccaaagaa cacgcaatgc gaaccttccg
    20941 cttctcacca ccagagaggg tctggaacga cttacttgaa cctttcttct gcacactaat
    21001 gttgaatttg tcgcgatact cacctttttt ggtgacatcc atcgtcgacc actcagcagt
    21061 aatattcccg tcagacaacg tattgagata ctcggcagtg cgtgtattca ggaaaggcgt
    21121 aacagacgtc aaaatatgag aacgcacccc ggcaggagag tagacctgac gagctttttc
    21181 caatagcaac gtttgttcct gaatagcctt caactcatct tttaaggact tgaaggtaga
    21241 cttactggat accaggttat cttcgtgtct ggcgataaga gcaataaacg gattaatctc
    21301 ttttgatata cgatcgacct cagtacgagc acgagtcaca agagactcaa tagcaacaat
    21361 ctccttctca cgatgacgca aagatgagag ttgtttcgta agctcttcaa tccgggcaat
    21421 gatagccgtt acatcaggtg tcgttttaac aagggcagac tcaatgctta acgctttctc
    21481 aagattcgtt ttgtgtttag ccattgcctc tgcaagtgtc ttcgcctgac caatttcctg
    21541 acgtgcttgt tcaatgaaat tctccttcac cgttgataga tcttcttcgc agtaggcttt
    21601 gccacaagta gggcatggtg atcccacttt agtaccaacc tcttctgctt tggtcttaaa
    21661 attacgcgcg cggttcattg catcagcctg actattttct gtagctttga tgcttgcccg
    21721 aatatcagtt atcgcaccac gaactttgag caacttggcg tcatgttctt cttttgaggc
    21781 taactttttg cgctcacttt cgattgcctt ctcggtatca cggatctgtt cgggaagagt
    21841 gcggatctca agttcaactt ccgttaactc aacttcggca cttgccagcc cagccagggc
    21901 atcgtcataa cgtttagaac gctcttgttc ccatgattca gaggaggctt tcgcggactc
    21961 aatttctgac tgtgttgcct caatggtcga gattgtcgac tccaatttgg ttttaaccac
    22021 atccatacgt gcggcagctg cattagctcg ctcacgagca atagcgtagg cgcgtgtcag
    22081 tctgtcaacg ccagcggctt cttctacgat ggttttgagg tttttgtcgg acattccagg
    22141 taaatctggc atagcttctt ggctcgcata gatggaagcc atgaaaacct ctttagatgc
    22201 accgatcaga cgctcgacga actcttgcgt cagcgcatct ttgcctttcg tcatatcgcc
    22261 gtcttcacca cgaacgataa gccgattttt gaactctttg tgtttacggt gacgaatgat
    22321 cgcatatctc ttgccttcat cctcgatggt tactgcaaca cgacagttct tttcatggtc
    22381 ggtagacaac acatcgtcac ccttcacacc atgagctgtt tcgccataaa gacaccacat
    22441 caggctattc attagcgttg actttccaga gccattactt gatgcggaac tatcatcact
    22501 attaacaccc tgaatgagca ctaaaccacg ctgatctaac tcgacctccg cgctggcgat
    22561 agccataaaa ttctcaacct ggagctttaa aaacttcata taacaccttt gataccgtgt
    22621 gttagctttt gaccttcttt caatctgaac tccgtatgcg ccgggaagga tctgcgcctc
    22681 aatacgcctt ttctggttaa ccttccaaga taaaacgcta cgggatttcc ttgtggcggg
    22741 tagggcttgt gaaacatcac cgtctggcct cgttcaagtc ttttcatcgt gatggcaaag
    22801 tctcgaaagc tcccataacg actttgtctc acacagcctc cgaactctcc gcttcggtga
    22861 ggatttcctg acacaaaata tccaacttgc tcagatcaaa tccgccgtca gtatcgtgga
    22921 caatcttgca ataagcagat accgactctc ccaggctatc gattttgctg gtttcagttg
    22981 tgctggctgt cccttccatc attgatgact tacggatgaa gttgcacacg acaccttttg
    23041 cgcccattgt tttgaggatg ttctggtact taatgccttc ttcatcgttc tcaattacgg
    23101 cgcggaaacg cacgtagttg ccgcgaattt ggtcatcagc aacatcatct tccaggttaa
    23161 tgaatttagg cgcactggtt tcgtagtgac tgaaactgcc gtccgggttt acgatcatgt
    23221 aaccagccag agatccaaca tctccccaat tttgatgggt cagtgcaccg acactgataa
    23281 ctccaggaat gacctctttg tggttgtggt aatgcccact aagcacgaga cgaaagccga
    23341 tatcctttaa ctcctgcgca tcgataccga cgtcaggcat tgttggaatg gctttgttaa
    23401 tggatgtatg gataacaacg tcatgattat caccttctac gctcttacgt aatgctttca
    23461 gatcgctgat aagctccgca tgattgttac gccagctaat caggtggaca gtcacatcac
    23521 caatttttat tgagtgtggg cgtttgccac atacgattac cacgccgata gaactcagcg
    23581 atgctgctgc gttggcgcta tatactgaat cgttggtttc aagatcgtga ttaccggcca
    23641 gcattactac tgtcagatca agctcgttga taatccactt gtacgtttca gttacgtaat
    23701 gcaaaacaga aggggacaca gttcctcgga cgtgaaatgt atcaccggca accaacatgt
    23761 acttacaacc ggccttcttc atggcgatgg ctgcttcttt cgttgcttcc aactgtattt
    23821 caagtctgga gttaagcccc tctgcgttcg tcgtggagaa cgcatcccat ttgtggtagt
    23881 ggcagtcaga aatgacgccg tatggcaaag tcatgtgttt ttcctttgtg gttattttga
    23941 tacaaatttt aagagcaatg agaaggtaaa caactatgca taacggtatg ttaggaatga
    24001 aacgagcgtc ttaatataca aaatttataa gtaagtatat acctatttgt tgtggcgcaa
    24061 ctttgcttgt ctgtatgact aaaaataaga caggatcact gtagaacgct atgagagcta
    24121 ttttgaggag gcgtagattc tatctacgcc aatatacgat ctggagggtt acagcgcgtc
    24181 agtgggcttt ttacgcgctg cgtttatgct tatgacgcca gcttttggac gtgggtcaac
    24241 aaggttaagc tcttcttcgc tgtggtattc gaggtcgaac tcacgtttta catgtctgat
    24301 gtagattgcg gtgagcaggc tatctttggt cagaaagtgt ccgtaggatt tgcggatcac
    24361 ttcttctacc ttctcgattt tctcaccacc catacagaga tgattaaacc ggctgtgttt
    24421 ccgcaacatt tcgtctactg ggcctgagta aaccttgtct actttcccaa aacggatgat
    24481 ttttcctgtg tctgcggata cgaggcaaat cagcttgtct ggtgataaac gatctcgcca
    24541 cgtcacacct gaacggagtg tgttgaagta gggggcatcc aatccgatga tcggtttacg
    24601 aaatgcgagt aatggaacgt acctgatgca actgtttaaa tgaaaattca caccagcctt
    24661 atcaagttta attctggttt cgtagatcgg gcacttcgcc gctataccgc agatgtcaca
    24721 aagcaacttc tgcttattca ggttggaatt tgattcgata gtgtaggagc cgtcttcaag
    24781 acgacgaacc cagcgcgtgc gttttagatc catatgtcat ttttctgtga ttgttagccg
    24841 aggcaacgat aacccaccag gtgcgccgaa ggtagtaaat gcctgtttta attattcaca
    24901 tatccacagg atagatccta ataaagagat ccatagatag atccttatat agatcaaata
    24961 agatccccga tcgctgtaag ccgcgccacg actggtctga agtcatgttc atgtatgctg
    25021 tcagcggtaa ttgatagtct gtcagcggtt caccgtatgc tgccaactgt ttttggtatt
    25081 ctgtcagcgg caaatcaagt atgctgtcag cggttgaatg acaaaggtgt ccacatgtcc
    25141 acaaaaaata aaaaaggtaa aaataacaaa gaagtagaag ataacatcga caactttgaa
    25201 gaaggttccc ttgaattgta cacaggggaa cttgtcccta atagcaacaa tacagtccaa
    25261 ccaattgcgt tgatgcgact ggggttgttc gtccccacgc ttaagggaac aaaatacagt
    25321 aaacgtaaca agccaaatga gatcgatgct tcaaaggagc ttgtccagct cgaagtagcc
    25381 cgttctgaag gctattccga tatcaagata accggtcctc gtctcgacat ggatcatgat
    25441 ttcaaaacgt gggtgggcgt tgtgcgttcg ctggcagaat acggcgaacc taacggtcgt
    25501 gtcgagttaa gcatcacgaa atttgctaag ttttgtggct atccatcgtc acagatccgc
    25561 aaaacactcc gcgaccggct tacaaacagt cttttgaaga tcatgcgtac aactctgtct
    25621 ttccagagaa cgtatgaaga aaagaacgtc gacggctcta acaagatctc gcttctgatg
    25681 gtgcacctca tcaatagcgt ggactacaac gagcagaaag atacggtggt gttctatgca
    25741 gaaccgaagc tggccgaact atatcgcttt gaccataagg ttcttttgca gttaaaggtt
    25801 atcaataagc taccacgcaa agaaacagca caggctctgt acaccttcat cgaaagtctt
    25861 ccaaccaagc ctgcgccggt atcgcttgct cgattgcgtg cgcgactcaa tttgagtagc
    25921 aggaatgtca gctcgcagaa tcagactata cgtaacggct taaaagctct ccaagatttg
    25981 ggctatctcg aatacagcga gattaagcgt gggcggtcga tctatatcca gattcacagc
    26041 cgcaatccaa aactcaaagt cgcaccgcca aaacctgaag acatcgaacc caaaaaacca
    26101 gatgagaaag ctggggaaat tgatgccaaa cagaacatta tcaacaagat aaccgaactt
    26161 tcgcagaatt tgacgcctga aaatatcaag atgattgaga tcctttccaa tagtctcaaa
    26221 ctgctttgat atgctgtcag cggtgaaatg tatgctatca gcggctattt ttctgaaaag
    26281 tatgctgtga acggttaaag atatgctacc agcggtatag tgagatgaac gtatgctgtg
    26341 agcggtaaat catctatcac cactcaatgc tctgtcatac ctccccagtg gttactaacc
    26401 tcgaagagtg cattccatat aggtttgcgc tcactaacat tctattgcta ttgcgctgat
    26461 tgcttgtagt gaaaggcatt tattgcctgc tactccctga tagaacgtat gctgtcagcg
    26521 gtgttttatt gaggaatttt tgtttggata tgctatcagc ggtaattaat ggcatatgtc
    26581 agacattcgt atgctgccag cggtaatttt gttctggtta tacccatacc ggtacacatt
    26641 attcatgcaa tgtcttaagt atgctgtcag cggtaaaaaa tcgagtaaag tatgctgtga
    26701 gcggtgagat agactctcga tatgctatca gcggtagttg attgtgtatg ctgtcagcgg
    26761 tgacgactat gaaatatgac tgcggaacgt gccgcagtca gtttgcattt agtgtatttt
    26821 ctttaacaga ccccaaagtg taccggcttt tgtagtcagt tttcctgttt ctggatcgta
    26881 catacgccat tcacgccgct ggtggacgat atacccatct tcacgttcca gacgctcaag
    26941 aatgccaggc tgcttgaaac ccttagcacg ccagtagccg cttgttttct cgatttccag
    27001 acctgccagt gttaatgcca ttacccaacc tccttgcagt catcgaaaag gtagctttgc
    27061 tgagaagcgt gaacgccata gaagcccttg tatttgttgt atacgaagtg gtcttctccg
    27121 acgccggtta atttgccatt acgatttgtg agatatgggg atgacaacac tcgatcgtca
    27181 cgcacaacat agaactggtc gccgctgtct acgaccatcg ctccatatgg tgcttttatg
    27241 acgtcggtaa gagcgccatt cttaacgtcg gctacagaca tttcacactg ataaacagtg
    27301 gtgtcggcca caacgatcga agaaagaaac aacagcgcga atgggatggt cttcatgctg
    27361 ccacctcttt gattacatca aatgctgaat taaatacgag cacgatagcc aaagcgttta
    27421 tgactacgtc gtgcaccggg gaaaagactt tacgaatgac accagtgaaa atgcagtcga
    27481 tgacaaaagc tattgaaacg atgagtaaca agtattcaat aaaaatcttc atgataggta
    27541 gatactaact tatataaact ttactgtaaa ctcactcaaa agtgctgatg cttaaaatac
    27601 gctcaagatg ttcagcatct tcattactga tttgttgagt ctcttctacg taccaacagt
    27661 tgccgttatg ccaacaaaca ccatcatcat caaccatcac tgaatcagcg tcatgacctg
    27721 tatattcaac aagagtacgc tggatcagcg cgtcagtttc ttcatcgtta aggccatcga
    27781 ctttgaccat aaaaagcgga aacacgtcat agacagtgct ggtaacgatt cgaactaaaa
    27841 ctctcatgtg tttttccttt tgtggttagt gtttacttat tttgttagtt atgaataggc
    27901 gatcaatctt cttggttagg gaaaaactgt tcaataactc tgtcaacggc tgtgtctata
    27961 tggagtagat gccacacaac catatctttg cttcccatgc cttgtgtgaa tatcacgtca
    28021 tacttacgcc atgcgcagtg gaaccaatat gctgagtcat caattggtaa atcctctccc
    28081 gcctctttga agtatttcag cagcattgcc tgctctgccg ttggcgttgg acctatttcc
    28141 tgagcgtaca tacggatgaa tcgctgccag tctgtctcgt cgggggttgg atctggtggg
    28201 gtcatttctg cgatagcgtt tcgtagctga tgcgcccaat caggttcagg aaagcctttt
    28261 gctacttcat catcaataac atccagagcc acgtttgcgg catcgtagag tttcttaatt
    28321 gctgccatgt ttacttctcc ttataaatag gaaaataatc acctttgaaa aaataaagca
    28381 aagatatatt cactccgata caagacgttt atatgcttcg atgtctcgac gtgcttcacc
    28441 gagttttctt cttaggtcca tattttctga ttcaagtttt tcaatgtttt tttggtatcg
    28501 gccacgatgt tcttcccagg catcctgata tgccttcata tatgtcgtat tagcctttct
    28561 ttttgcctga cgaaccgcgt ggtgattttc tacaaaccag tcagggtcgt taaatgctgc
    28621 tcttgcgcat ttataccaat aatcggttga ctctctgttt aaccaatgaa tggtgataaa
    28681 tggtaatcga atagacagaa tttttcgttt ggaatctcgc tcaccaaaca tatgcccttt
    28741 ttttatcttt aaaccgaacc caggttgaat taaaagcatt gtcagtacct cagatgcatt
    28801 tatggcagta attctttacc gctttccatt ttggtgatag agacagtagg atcatcgtta
    28861 atgcatgatt ctgtgcctaa tgctgctaac cctttacgca taaccgtgcc cgcaacatac
    28921 gcctcaatct ccgcctttcc agatgagaca atttcatcca tttgttcctg cattaattcg
    28981 gtagcaaagc acagattttc aggaaggtta gctacagcag atgctagttt tctatatagt
    29041 tcttcaaggt cacgttttcc agctcttcct tttttcacaa gttcacctag ctctgaaact
    29101 tgctgattaa gtcgatcaat ctcactattc gtagcggcct ttatttgatc gttgaatttt
    29161 tgccgaatac ttttttgctc ggcaatatca tgaactctaa ttgagtctcc aatggtttta
    29221 tatgaaatag tgcatggcac accttcgccc actccaaaac tgctaaccat agcagcccat
    29281 tgtgcctggc taagtctaat ttcaactaaa ggccgtctat tgcaacttct ttgtgggcgg
    29341 gttcgttcat tgtctaagga cagttccgcc tcgtaaacgc ctatttcaat atagtctcta
    29401 tgttccagtt ctgaatcaaa taggcgaatg ccagttgtgc taacacggct tgttttcaca
    29461 agaccgaacg ctggatgtgt ttgaattttt tccccatgtt tattaagggt cgtttttggt
    29521 tcttcaattt ttcttttcat cttattaacc tctatgcttc atgcgtaaag ctaaatgaca
    29581 atttttttta atatccggcg tattcgtatt cttcatttag accgtattct tgtggagtga
    29641 taccaggaca acataactca aaggcgaact tgcgccattt gattttttct tctgacgatt
    29701 gtttgttatt cgccggtgca atgtagtcat attcccattt gccgttgcga agtaaaatga
    29761 atccgcagtt attgccgaag tcttcgtctg cataacgtat tacaaactca aggtgcggga
    29821 actttctagc catcgcttga taaattggtt ttggcataga ccaggctgtt tcaaagcgaa
    29881 tcaccaactc accaccagtt gcggcatgtc ttgcaagacg ttttttatag attcgttttg
    29941 catatgctcg aacgtgtgta tggcggaaat tatggccgta cttaatacgt gtagggatac
    30001 gttttacggg catttcgatg ttgtaggcat tccattttgt tccccaatta gctcgcgacc
    30061 agtcaaacca tgagtaatag ccatagcgac gataattctc aagccgcata agagcctgct
    30121 ctttgatttt gcgaatttcc ttgcgtgtca ttcctgattc acgcatagct aattcgacct
    30181 gttctggagt ctttaattca ccgaacatgt ggtcgtaaac tgtttcgccc gccattgcag
    30241 aggccatgcg cctgatatga ttgttttctt ccatttctat acttttcgga cgtctgcaaa
    30301 tggtattaaa gtcaattaga ccttttttgt tagttatggc acgtatgaaa tctaagcgtt
    30361 gtttatttgt accacctatg actcggattt cgtttgtaat gtggttgggc ataattaact
    30421 taacctttta acaaattgtt ttcttgtgtg tgataataac gagaataaaa cggcgtccaa
    30481 ctggacgcct gatcttctta atgagagtta tttcactgta atgaaaggag tgttagcccc
    30541 ttgagtcatg tactgcggta actggccatt ccatttattg atggcttcca gttccataac
    30601 gttcgggttt tgacgcagag cttcaccgcg cagacgaatg gcgtctgctt ctgcttgcgc
    30661 acgagcacgg atagcatcag cttcaccatt agcctgttca cgtagcatat tcgcctcagc
    30721 tttacgttgt tcaacttcct gctgacgttg cagagtacgc tggttagccg ttactttggc
    30781 gttgatagat tcaatgacgg tttttgggta atcaggcttt ccaacccatg acaggctcag
    30841 tacctcaata ccaaccggag acatctcttt ctgaatatct ttcagtgcgt tgtccagcaa
    30901 ttgcgcctta ccaccgtcga taaatgagtc agtggtcata cggctggcca aacggtttag
    30961 agagtcggca attttctgac gcagatctga ttcggtgata tcgtctacgc ctttgcgata
    31021 ggtctggaac accgtcgtta ccttgtcacg attaaccaga tacgcaacgc caattttgtg
    31081 accaatagca gtaccgtcac tcatctggaa tgtgaacggt tcgtcgtagg tcttcatttg
    31141 tttgaaggtc gggaatacgt aaagttcggt gttaagacct gtccattggc gaccaacgcc
    31201 aaccacttca ccgatccctt tgtcttcacc taatttgttt actttgatgc caacgtatcc
    31261 aggctccacg cgatcacagc cagtaagacc cattgtgcaa atagccgcca gagcaactgc
    31321 aagtaaacct ttcttcatta cttatttccc ctttgctttg attaatgagt tgataaactt
    31381 acgaccagca ataagaccga ttacaggagc accaaaacca acgataactc caaacatgac
    31441 ggctaaatca ctcttggtag aaatcagcga cggaaccagt aaaccgtaga tgagagccac
    31501 tgaaacgcct gtggctactg ccaatagata gattttgatc atgtgttttc ctatgtggtt
    31561 gttatgcgaa aataataagt aagtacttat atattttcaa gagataaaaa aggcgtcctg
    31621 tggacgcctt gtgtttactc ttctactgac tcggtggggt tgtcttttgc tcttcggtcg
    31681 tcgattgcct gcaaagccgc aatgatttca gccagtggct tctcccgata catctcgacg
    31741 atctgcgatt tggtgtattt cttgtcacca atttcaactc gcccgctggc gttctttggc
    31801 aggtatcctt cttccagcat gtactcgacg agcgattcga taacgtcgag gccacgagta
    31861 ggatcgaagt agaatttcca ggtgcatttg ccatacggtg gtgcaacttt gtttttgatg
    31921 cattctgcgc ctacgtcctg gccgatcttc tctttaccat ccttcatcac cgatgcacca
    31981 agacggatac gtacagaagc gtagaacttc ggtgaatctc cacctggaga cgtagtaggg
    32041 tcgccaaaca ttacaccgat ttttgtgcga acctgattca agaagatgat gcaggcgttg
    32101 tatttgcgtg cccacaaagc cagagtaggg aagttcgcac ttgtcgcacg agccagtgcc
    32161 gtattgtcgt tcatgtttag ctgatctttg tctttggcag tgccttcagc catcttttcg
    32221 aacttctcgg ctttggagtt cggaaccata gacgcaagtg agtcagccac gatacagata
    32281 ggtgctgatt caggaataag ctcttcatca cgtaccaatt taagaattgt gccgatcaac
    32341 tctacagagt cttcgaaggt atcaggctgc ttgtataccc actgaccgtc atcctcatct
    32401 gcattcaaac cattggctac agccaggcca acgtcaaaac tgttctcatg atcgaggaat
    32461 accgccagac catcttgttt ctgtgcggag atcattgctg ccgttgccaa aaaggttttc
    32521 cccgcgcttg gtggcccgaa tacttctacg atacgaccac atgggaaacc gccatcgtaa
    32581 cgaccggaaa tcgctttgtt taacggagga aagcctgtgt caatccaatg agttactttc
    32641 tgaatttcgt cgttgctacc gattttcttt ttcagagcaa gtgccagtgc ggattttcct
    32701 tttgccatga tcaggctcct tttgtttcgt tgattcgttt taaagcggcg gattcgtcga
    32761 atacaatcgc atcgtggtta aggagtctgg atacacgagc gaggattttt acgacctgct
    32821 cgctgactaa tccaaactcg cgatctgtcg ctttcattcc ggcagcgcct agaattgacg
    32881 gcagtgcgat gacagcgtac tcaccgtgat aaaagacaat ctcttttgcc agttgggtag
    32941 gggtggttgt agcgccatta ataatcgatt taagcattag caataccttt cgaatggaag
    33001 aacaaacact tcaaggtctt caaggaacga acggaaattc agctcgtagc acagttgctc
    33061 gaatgctttc acgtcacgat tgcctttgat tgtttcgatt tcggtaggcg gaaacttcgt
    33121 ctcaatcagg ttcattagcg tgatgtttct cttgaacgct tcgagcattc gacagcctgt
    33181 tttctcgttg aaggcattct tcgctagttt gttgaaggcg gtcttatggc gtcctttgtc
    33241 aaccacgatt gagccgtcgt tgatgccgcg taccatcgtt gcgacacttc cccattcatg
    33301 cagcagctct ttcgcaccgc cagcaccaat gccaccaaca ccgctaatgt tgtccgattt
    33361 atcgccttgt aatgcttttg cttccaaaaa tgcgcgagga gtagcgaatc ctgtcagctc
    33421 cgcaaattgc tcaaaattaa cctgcttgtt tttggcgtct tcgcgcaggc ttacccagct
    33481 tacgttttcg cgaactaact gaagccagtc gctatcgcct gttaacagat agatgtgttc
    33541 aacaattggc tgcggtgcaa tgctggctac cagcagcccg gccagatcat ccgcttctgc
    33601 atcttttgca atgagttggg taactccaag cgcggtcatc attttgagga tgtatggctt
    33661 ctggatagca aagccttcct tcatcttttt catatcagga tcgtcgtcgc gatttgcttt
    33721 gtagtccggg taaaagtcgc gacgcttgtc actaaatcca tcccaaagaa tcataggtcg
    33781 ggcatggagg atggaggcat aacgacggac gttcttaaca aagccgaaag cagcctgtac
    33841 ttccatttcg ccgttgtgca atttgtcaga ttgttggtgg taataaccca ggctattgcc
    33901 atctacgaag agataattca ccggtacatt ccttccaaaa agtaaggcgt ccgtagacgc
    33961 cttactggtc acgtaatggg attacagaga gtccagctct ctcagtaggt catccagacc
    34021 ttcatcttcc gtcgcagatg cggctgctac agatgtcgct gcgactgctt cagactcttt
    34081 gactgatacg gcaacggcag cggaacttgc ctctggttta aattcagttt caacggcacg
    34141 gaggatttct tcatcaacca gactggttgg ttcagaagcc ggtgtgtgcg cggttgctac
    34201 agccgtcgct ccttctgtat gaccagtgac agagccaaat ccaggtagtg ccgcagccga
    34261 tgttttcgcg gttgaggaaa ttgctggtgc agatgctgcg gcagttggtg cagcgatgcc
    34321 aatcagacga cccatagtgc gaactgtcga cagaagacga gtttcatcag cctgatttgc
    34381 gtatgcgatc agatcatgct gggtgttcca gaatttatct tcgatatcgc ctttgtagac
    34441 tttacgctta ggcgagacgt catatttggt atcgcgacca gagccagtac gtttaatcag
    34501 gaatgcgtag ccttcctctt tgctcagtgg attgccgata tcatcagcga tgtcttcggt
    34561 gattgctttg cagatatcat cgaatacagt agacggcagc tcgattaatt ggcatttctc
    34621 agcatctgcg aaatcctcac gagcagaaag aatgccgttg accaggtagc gaggagtggc
    34681 acgcatttga ccgatgcgtt cttccattgc tttgttaccc ttgtgacgag cgcgaccttc
    34741 catcaccatt tcgcatagct gacaagcgcg accgtgagta tgttgctcac aaatataagc
    34801 gttggttgct tctttacctt cctcgttctg atacttaacg tagtgcatac cgaaagtctg
    34861 gaagaactta ccgtttgggt cgtctttatt cgggaagatg cggatatagt tgacgccgtc
    34921 ttttaagcga gtcagatcaa cgttgttgcc acgtttggcg gcaatgtctt cacgagtttt
    34981 gttaagcaaa tcaagtaatg tcttagacat gtgtttctcc ttgttgtgat ttggccgatg
    35041 gcgctatgcg cgttgggctt tcgttcattc gtggctcttt cgagctgtta aatgatagat
    35101 cagtacttac ttattatcta tcaaaaatca acggggagta ataaagcgtt cagagcctaa
    35161 tcgctctaat tcaacgatgg ccatctttga cgcctgaacg atcatgtctc ggcgatgcga
    35221 aaaggcggcg acagcatgtt tgtatatgtc tgctatgtgc cgtgcgtcat ccaacttctg
    35281 acgtttagat agatactgtg ggttcgtttt aaccttagcg tccagtacag actcgttgaa
    35341 cttaatgccg ttcatactca agttcttacg ctctgtgtcg tatagctttg cttcaacagc
    35401 ttcaaggtta agttttgcct ctgcgacact tctttctgca tgagcgagtt ttgagccata
    35461 ctccattaaa aggcggggtt gtctacgcca gacttcctcc agattgtctc ggtcaaactc
    35521 gagatcagac attattttct cgtaaatatc ggtgatcatg tgctttcctt tttacttatc
    35581 atcttatatt gattatatca ttgtgatgat aaggcaagga gttcgaggat aggggatatg
    35641 aggggataag agaaaacatg gccatagagg ccatgtttat tggatcacga cttaatagtt
    35701 tctgctacgt cggctaatat cgcttctagt cgttctccct cttcaggtct gaagtaaaga
    35761 atgttggggt taaacccgta aaagactgta acgtcaaggt ctggaagata ctccttccta
    35821 ccgactaaat cggatggctt gtttttgttg ttaaacaatg ccgttgcccg actgccacac
    35881 gtcagcacat aggtcggacg taccagattg atctcttcac gcataaagtc ggtgaactgg
    35941 ccgatctcgt ctttggtgta gtccttttct ttgtctttca cctttttgca aacgcctgtg
    36001 acatagagat cgcccatgcg caaatctcca gccgtcaaca gcttggcctt aaaatcgtcg
    36061 tatccgttct ccatgaagta accggttcga gcatcattgc cgtttgcatt gtccagaatg
    36121 atcatgattt tcggcttaat accaatgctg gggcggatta gttcatcacc taagcccatt
    36181 tcagccgcca tccgcgtcat aagtacgtta atttcagccg aacgtttgga gttcatctca
    36241 aacggacgtg aggctttaac tgcgtctatg acaagatttc ccatcaattc cgcttggtcg
    36301 cggagacgtt ctggatcagt tgctggcaga ctgcctggct cgatggatgc aaacgcccct
    36361 actttttgca gtgattcgcg cactcgacta ttacaggcac gtttctcgac agcttcttcg
    36421 aattgcgcca gtgactcgaa tttgccgcca actttttctc gcgctctcat gatggcctgg
    36481 cagccattct cagaacaacc tttcacagca gagaagggcg catacagcac ctggctgcca
    36541 tcttcaagcg tgcggatctc aattcggtta gatgacacgt taacgtctgg tggcaatacg
    36601 cgaataccat aggtcagcgc atctttaacc agcccctggt gtttatcttc gccaagaata
    36661 gtgagagcag cagcgaaaaa ctctgcggga taatgagttt tcaaccacat agattgataa
    36721 ctgattaagg agtaagcaac agagtgtgat ttgttgaact ggtaggcacc attcttttcg
    36781 aatgcggtcc agatctcctt cgctttcatc tctgatagcc ctggatgtga cgctgtaacg
    36841 cgtactgcgt ttataggtag ttttgcgccg tactcaagtg cctcttcgac agttttcata
    36901 gttccgtctt cgcatttaaa atgttccgca cggtgaatgc gctgtgtagt gccatcttcc
    36961 agctcaacgt ctatccagcc agcttgagcc tgaacgatga acttctcgcc catgctcttc
    37021 attttttcca tgtctttttt gcctatcgct ttacgaacgc cgtcagcttc agccattgtg
    37081 aagccagcaa gcaatcgagt cgcgttcatc gtctgttcct gatagagaat cacgccattt
    37141 gtttctgcgg taagttcatc aagaacgggg tgtaatgatt ccggcgtcat aaagcctttg
    37201 gcgacagaaa cataatcatc caacatgcct gactgaattg gtcccggccg gaagagcgca
    37261 gtcgtggcga caacggtttt aaagctcatt ggctcaatgc caccgcccag atctttaagc
    37321 agcttgcgca ttgggcctga ttcaagctgg aaaacacctt gagtgtaccc agcagcaaaa
    37381 ccatccagca ccttgcgatc ttcaagagaa atggtatcaa gattgatatc ttcacctgtg
    37441 ttctctttaa tgtagcgttt tgcactatcg agcaaatcga gagttgccag gccaagcacg
    37501 tccagcttaa ttaggcccat agcctcgcag taacgcttgt caaatgcgat acaacgcgcg
    37561 tcaccacggc gctcaaccgg tgtacgttct gtcagaggaa cacctgctac gatcatccct
    37621 gccgcatgtc taccaaagcc acgcataagg ctttgtaact tgcaggctgc attgaatgca
    37681 tcaggatatt ttgttgcgta tttgtcgaga cttgccagtt gttcgcgcag ctcttccaat
    37741 ggaaggctat catcctcgac gttcttcagt tcttttgata ccgccatatc tgcggactcc
    37801 acaccataaa tacgagcggt atcacgtagt gcagaggctg cgcccaggta ggtgaagttc
    37861 ggaatgcctg caacgtaatc ttcgccgtag cgttcattca gatactcgat cacttcatgg
    37921 cgacgtgcct ggctgaagtc caaatccgca tccggcaagt cgagacgctc agggttgatg
    37981 aaacgttcaa acagaagacc gtgacggatt gggtcgacgt tggttatgcc tatgcaccat
    38041 gccaccagag atccagcgga ggaaccacga ccaggcccga caggaatgcc agtttcacga
    38101 ctatgattca tcagatcgcg caccatcagg aagtaaccac agaatcccag gcgagtcagc
    38161 gtgtccattt cgtactttag ccgctcaaca taaaccctgt tctcagaagc aggtggtgtg
    38221 tatccaaact cttttgtggt aagacgttta cgcagccctg caacagccag tttcatcagc
    38281 gttgcaggtt cgtcgtctgc catcttgggc agtgctggtg gcaattcatg ccagcgccat
    38341 gtgcaggcat cgataatggt atcctgcgtt gttgaggcca ttgcagctgt taccggcaca
    38401 tccatgcgaa cggagaaggc tttaagcgcc tcaaggagat ggcggcgacc attgacggcg
    38461 ttatctcgct ggtgggggat acgcagacga tgcggctggt cgatttttat gttgttcgta
    38521 accatgtgcg caatgtcttt aatgtctgca tcgtcgatcg attcgtaata agcgggatag
    38581 aacgccactg gctctatttt caatgcgctg gcgactttca tcgcccggac gttaatctgg
    38641 tcgtagaatg gggtagggtg cggataaacc acactataga agttgtcttt tccacccggt
    38701 gtaatcagtg tgcttatgat ttttgcaaaa tcgttgcgtt ggaacacgct accgatgtcg
    38761 gaagtgagca ggatgatgtt gcctttggca tatgtagaaa ccaactgttc gagcgagaga
    38821 cgcggtacaa agtagaactg ttctcgtgtg ttggccaccg tcattagttc gcagatgtcg
    38881 cgatatcctt gctcattttt aattagagcg gtaaacgagt agttacgtcc tcgctctaat
    38941 gattccatac atcccttaga ttctttagca agtttatccc ggtgctcgta tgtgggatca
    39001 tcaacgatgt ttagtttcac accacaaata accgccatgt cgtcaccagc ggcacgttgt
    39061 agtggaataa cactcgcaat attcatgcta tctgctgaaa ttacagcggt gtagccagct
    39121 tctctggcaa tcttcaccgc gttttctgct tttagagccg actccccaag tgagaagtca
    39181 gttcgaacca tcagtgcctt catgtgtttt tacctttctg gtttttttga ttttgtcatt
    39241 tgggaagcct acgaacttcc catgcatcga aatcgcaact tcttttgctg attggtgaca
    39301 ataaggcttg tctggacact ccagacaagc cttaccagtt tcagaagcag cgataagaga
    39361 gccgaaacat cctttacgca cgatcaacca aatatttttt gaacaacttc acgagccgct
    39421 tgtgcagaag ctgaagggag tttgttaatg aaagaacgct caatacctgt cacaaaatcg
    39481 cctcgcatca ttccaatctt ggccgacaac aaaagctcac gaggcccaat cggttggctg
    39541 atattgtgtt cttcgtatcc atcgcgaacg ataccggcaa acttaaccat cttttcagcg
    39601 tattcatcca cgatgcctgc atttgtcaac atgttgatct ctgcgtcttt gctcatgtat
    39661 ttcacattcg aaacgatgcc aaaacgcgaa aagttcgcgg cgttctggat gtttgtaccc
    39721 tggtacaagc cggtttcatc accagatccg ttcgtgttgc cagtgccaat gaaagcaaac
    39781 cgttcatgcg gagtaatgcg acgccattct ggagttgcct ctttgatgac caacgcttca
    39841 ccttccagca ctggctgata cacgccaaga atttgtggaa acgcaaagtc gtattcatcc
    39901 gcgaggtaaa cccagccatg cttcattgcg agcgcaagca agccaggttc gaaatacgta
    39961 gagccatcac gcgccagaat ttgccccgta acatgtgcct cttccattga tgctgtatgt
    40021 tgggcacgga tcaacggacg attgagcaaa gcacatagct gtgtaggcaa tgacgatttg
    40081 cctgttcctg catgacccca caaatagcca ggtatgccaa gttcaagcat catgaaaata
    40141 tctttgatca gttcgaagtc gccatatacg tagcctttct tcacttcggg aacgaactct
    40201 ggatacggcg tattgacgtt gacgctaacc atgagcggcg tcccacgttt tgttccgagt
    40261 tcttccaccg ttacgtttag cagttcgtga gctgcgacca gatctgtctt gtactcaact
    40321 gtgcctgtgt agccagggct actggtcaag ccagaagatt tggccatttc gcttttttgc
    40381 tcggcacgtt tagcttgaat tgcatcaagt gcctttttcg atagcgttgg ctcatctgga
    40441 aactgtgttg tgtacatttt cagtacggtg tcaggatcgg catcttttac tgactcagga
    40501 atgccctcgc aattaccatt ggctacatgg gacttaaaat agtgaaatga ttttccgcac
    40561 cacttgcaaa caaggtaatc agaaggattt ttttcatttt gcagtgcagt agtagtcatg
    40621 cgtttttcct ttgttttcta atgagctttc aacttcttat ataaatatac catcaaatat
    40681 cgtaagtgat tacttatcat taaggctatt ctaatcactt caaaatgata cgagatagtt
    40741 cagtaactac agatgaacca agactatcaa cgctcgttac cagggcatga tttgagtaaa
    40801 atctctccgg tgcgtcagtc atgatgccaa ttgccatcaa atcaatgtca gtctgcgttt
    40861 caatttgctt cgcaaccgaa cgcaaatgag cgtcaaaacc tctcccaaca gcccacggcg
    40921 cgccatcact cagaacaagc atgatcttcc tgtcctccat gcgaccggaa aataacgatg
    40981 caaggctggc tatgctttca ccatccacgt tattaagaag tgggaatgtg tagcctacgc
    41041 accccatacg tgagcgaact tcgcgagaac ttaccttttc attccaattt ttgatgatag
    41101 gtagcattaa agattcaaag cgagagaact catacttgac agccttaagt tctcctgccg
    41161 ccatatgact accaaatgta gtgaagccgg tgataatgtt gggaacattt attcgatcaa
    41221 gggcatcggc gatggtatat gaacttgcaa gagccaattg tatcgttata ccactcattg
    41281 aacctgataa atcaatcact tgttgaacac aggcgttaac ggctttagat tcttcttttt
    41341 tacgaaacac gcgatcatca ttcattgcta atctgtagat actagaacca tgaatgcgcc
    41401 cccgacgctg gcccggtata aactgaactc tgtttctgct tgctattgct cgctccaggt
    41461 cttttgccag cgtcgacgaa acacctgacg aaagatgctt ttcaatatat ttgtcgaaga
    41521 gctggttgcc ttcaggaacg atgcgatagc ggctgattgg atatcctcct aaatcaatat
    41581 cggagaatgt tttaataagc cgtttgatat gggcttctgc ctgatcaatc gagccgatga
    41641 agtcgtatga gcgattgtat ggcctgtatt cgcttttcga gctttctgtt aactcgcttt
    41701 taatcgtttc ggatagagca tcttctgtca tgcctccgac ttcatcttct atgctatcca
    41761 gctcctctaa agcctcttcc agactcattt ttgacggagt tggaatatct gaagagccgt
    41821 cttctgtcgt ttttcttttt tcgtggtcag cggttaatga ttctgacgcc gcgttatcag
    41881 aagcatcaga atctgatacc tctttatcat tttctgtatc atcgaatatt gtttctgatg
    41941 attcagaatt atctgaatca tcactgtcgt tattatcatg tttgtgctta ctgtgtgatt
    42001 ctgattcgat agatttgtga ttatctaacg ctgagggtgt ctcatgatct tcgttgtctt
    42061 cattgttttc tccagaggca tccgtctggt cagcgtctga aggatcggaa gtttttcccg
    42121 gagctgactc cttacctttg ctttcacttt cagtgtcttt gaggatctta gctatggtcg
    42181 cagccacctt tacacaatcc tcggtgcaag acatgttacg cacggccaca tcgataccat
    42241 gttcttttaa taatgaaatt ggtttctcaa tgacaggcca gtattcatcc atgaaatcta
    42301 cgaacggtgc ttggccatcc caggctctta caaccggaca gagaaagaag tttagaaaaa
    42361 gcgcgcgctg atctttgcca caataagcaa tagcctctga agcctttggt ttaaagactt
    42421 tttcgattat aaggcgctga gttgccatca gattacgtct cgttccgtta aagacctgcc
    42481 ccatttttcg ctcgatgaac acgtcttcta atgcattccc tagtgaccgg gaaggaacgc
    42541 gtcctttttc tctcattttg ttggagacac gaatatcggt aaacaaaatg tgagcaacct
    42601 catgatcgag aaatccgcgc acagcattca tcaatgccgg tgttgcgtta tccggaattg
    42661 atgggatgtt tacgagaatt ggctctccat catcgttata gcgtacataa gcgtcgtctc
    42721 ctcgttctgc aacagggatg tttttacctg aaaggagagc gactactcgt tttacactgt
    42781 cacggaagtc ctgaacctct ttgatggaac gttttttaga catggctaat cctttgttat
    42841 gaaaacaaat tatttactag tgtgtttaat gtagcactgc gcgaacaggg aactaagcca
    42901 ttcgcgcagg tagcagaggg gttaacaggt tctgacggct aaagagccag agccggtatt
    42961 gataagcgtg aagcgtttgt tattaagtag gaatataaag gctgttgcgt catgcatacc
    43021 aacctgaatc tgctcatttg gtaattcggt gagaatgtca gctacacatt catcagctaa
    43081 tttttcaacg tgtccgatct caagagtgat taggctggaa atagttgttt tcattcaatc
    43141 aatccattct aacttaaaat atggtaggga gtactattaa atcttatatc ataattgaac
    43201 ttatctatat catatctgag ttagttttgt tacatgttat tagtcgtttt tctccattaa
    43261 tgaatgctga ttggttaata tttgtgcctg tagaaaaaat gattatatct ttacaaccct
    43321 aacctttgat gtaatatcgg taagcactta ccagagagtt taacgcgcaa aggtcgcaat
    43381 aatgtctgac aaaaacattg aatttataga aagtcgctat gctgctttta tcgcagggct
    43441 gattgaatcc tcacctatga gtcaggccca aatagccaga cttattggtt ataaaaatgc
    43501 caacaacctt tctttaatta aaagtggtaa aattcctttg cctatcgata aggttcgtcc
    43561 gctggcgttg gcgctgggta ttgagccaag tcgtcttatg atgatggtgc tggaagaacg
    43621 ccaacctgaa ctcgcagcat ttttatacaa agaaggtacc gctcctctta acgaggacga
    43681 aaaacaggtt cttgctgcat ataacgagcg attcggtaaa gagaaaggcg catcacaaaa
    43741 ggttgttgag gccataaagt ctctatgaaa aatttagacg aataagctct gttgatagac
    43801 gatctccctt gaatttgtgg tcaatttcgt ctaaatccgg ttgctctacg attgatgcga
    43861 tgtacgttga gaaactttct aaggcgtctc gcatctcgtc catataatcg tgccggtcgt
    43921 agacccgatc tatcccctca agactgtggt tcattatttt gcgtgatacc tcctggctta
    43981 tgcctaatgc tgggaagtaa ctacgcgcag tacggcgcaa atctcggggt gtaaatggcg
    44041 caagctccat cagttctggt cgttccagaa tacgacgtaa tgcctgggct attgccactt
    44101 tagacatagg aaggtctttc ccgatttttt tattcgaagg cacgagccac tggctgtctt
    44161 taccgtattc gaacaactct tcaatgcatg tgcgcattaa tgtgcttaaa ggtagcgaat
    44221 gctcacgtgc tgacttgttt ctcttgcctt gattccaaac tccacgctta agattgaact
    44281 cacttttttt agccctcagt acttcgtcag gtcgtcttgc tgatacaaga catagcctgg
    44341 ccgcccattt tgtaccagcg cacacattga agtagtccca tatattccag aacacccata
    44401 cctctgcgtc ggtcagcttc cgctcgcgag gtgtgggctt tgcgccaccg gcaactttgt
    44461 ttagtgacat atcgtttaac ggtgacacgt ctatcatccc ctggaaggcg caccagctaa
    44521 ggaactgctt catcagagag aaaacgcggc ggcccataac aattttgcca tccaatatta
    44581 gagggttgac cagttggtta accatgaacc tattaatgtc acttactttt acatcagcaa
    44641 tgtgcggcaa aacatgtatc aaaatacaat gaacagcgat ctctggccga cgtctggtta
    44701 tcagcagaga taagcgagtg aataacatga atgcgtccga gaatatcatg ttattgttga
    44761 tctgtgagat cataatggcc tgcatttgag atgctctttc gagatactca attgcctctt
    44821 ttgaggtgtt ttctgctgcg cgtgctctgt caaagctatt tttcatttat tggcattcac
    44881 ctagttacgc tgatcacctg tataagtaac cagtatatta ggcattgatt cttttggaat
    44941 caagctaaaa tctttcatgc ggaaaatgat tccataccta aagagtatgg aatcattttt
    45001 ttctgtagag ctagttttaa atatttgatt cgatatgaag ccaggcaatt tcatgcctgg
    45061 ctgttacttt gtgttaggtt gggttgttga tgctaatggt aagtgtttcg atagcaagat
    45121 catgagtttg gttgtttgtt atatctgtta aaacaaaagc ctcatcacca agagcaatat
    45181 tgtctttaac cagcttgaat ttactccccg ccttgccatc gagaacggga attacagttc
    45241 ctgtgccttt ctcatattgc aactcaagag tgtgccaatt gttgtcgtag tcaccatatg
    45301 agtctatctg aacatttcga ttattgcctg tcctgtgcat cataatgttc aacttgcctc
    45361 caattgtttg aaggaagaag ttagccagaa acaggctccc agtaccgtcg aatgatatat
    45421 cgtcagcaac aggtttactg gttttgtaat atataccaaa gccaaactta gctttttttg
    45481 gatcagggtt tccagataat ttaaacttac aaagtaattt acctccattc tcaagaagag
    45541 cctgtgctat gtcagtagct ttactgatcg tccaagtttc gttggaaacc gctttagtta
    45601 aagcaattgc ttttccgttt tcaactgact gaatatttgc accagttaca gtccatcctt
    45661 gttcagtagc gacatcacca ccagctccag ttaaggaaat aaccgttcgc ggggttggag
    45721 ttgtgacaat gttgtcattt ttaggttgag taggttgttc ttgtttaata atctcacctg
    45781 taacgccatt cacaaaagca gatgatttcg ctgaatgtgc aagcagcgca gttgcgagac
    45841 gatcagatat tattccgcgt cgctgccatg aaccaaaatg actctctcta ctctcggaag
    45901 tccatgtttt attatccttg cgatgaccgg aaccataata gccaatagat gtgatgtctg
    45961 gatcatctgt agggctattg gttaatacgt tattatcatt ctcatctttc atgaatggaa
    46021 cgaaataaat tgcttgtgag gtttttccct tataaccacc ataaacaatt tcgtactgtt
    46081 ttggatattt gtttttccac cagtgggtcg tatcgccaca aatccagggt acatttgcag
    46141 cacttccgaa aacacattgt gcgtcgtatt gagatagctc ttttctgaaa ccttcgacca
    46201 tcttgaggaa cagttcgtta tgctgcgagt acttgccagt tgccaaatca gcttcgcctt
    46261 gcatccagac aacaccaaga aatacattct ttttgttttt acttaaggcc gcttttgttc
    46321 tggctacaat atccttatac aaaggagtat ttaatcccca ttttaaagat gattcgattg
    46381 cacctgactc ttcataatat tggccttcag agcctgtaac agtgaaacca gaaccacctc
    46441 ttccgcaagg aacgagcaaa atacctgcgt ttgctggaat gaatggtaat agttttttgc
    46501 caatgtgtag tccctgacca acacatccat attcagagtc gtttgctttt ggatgcttga
    46561 atttagtcat gtcttgtaca tcatgaagac agtgatcagc gggaattatt tcattgtact
    46621 tacaggcaaa accacctggt ttaatattgc tgcgtcgagc taactgtttt acgcgaggat
    46681 gtggccgatc aaaagttttt ggcataggca gtccttcgcc gtatgacatt gagttggatt
    46741 gacctgcgat agtaactaca taataatagt caggctcttc atcttcattt gataccggtg
    46801 tgggcaattg tgataaatca ccagatcgca caattgcttg attgtacgca tttggtccgc
    46861 ccttacttgg ttcaacaatg tttggtgact cgaaaggttg ccaaccctct gcgattttct
    46921 ccgcaagttt ttctctaagc tctttgcttg atgatgctga caaaatatca tagtcaatga
    46981 atgacataat cgttactcct ttaacgataa actttagcta aaatcgtatc gattttagca
    47041 gttaaaaaac gacaataaaa tataagagta atgtatgaaa gaacaataaa aattgcgtaa
    47101 atggatatgt gtaaaatatg tggaatttca cacacagcgt tgagcggtct ggtttatgtt
    47161 agcggagtct taatcgagtt gattaaaagg ccaaaccgct caacgctgtg tctggcggag
    47221 agtaatggaa tcgaaccatc atcgcttgcg caatgggacg gttttcaaga ccgcttgagc
    47281 accatgctcc ctactctcca gtcgttgtgg ttaccggtac tgatctccgg cttagcgacc
    47341 ccggctactg actgcattgc agtgtgtgaa ctcgcctcgt gtgtcgctgg ggcatttctt
    47401 tccgcgcatc agtctgcgca tcaaccacaa cggaaagagc attcacgtct cgcgtgccac
    47461 cacgaatggc aactcagaga gtcgaactct gaaaatgttc ttacctgttg tgcgctccgt
    47521 ttcgtggagc taacggcggg tgaacaggcc gcaccaggtt ggacatattt cagcgttatg
    47581 ctgttgcaag agagccaaat tgtgatggtc ggcgctgatc tccgacacag ggttgtagca
    47641 agccccgcaa agcgcgcact actgtagttg cggcacatca gcctgtgcat tcaccacaat
    47701 gttgagaaca ctggttgtca cgcggcaacg caacatttat tcatagattg ggatatgacc
    47761 ccgttacgcc aatgttctca atattatgtt ggtgccgatt aacgggatcg aaccgatgac
    47821 cattcgctta caaagcgact gctctaccaa ctgagctaaa tcggcaattt ggtggggagt
    47881 gatagagtcg aaccacccga gtcgcaatga cagtagattt acagtctacc ccgctacctc
    47941 tacggactaa ctcccctaaa ttggcgatgg tgggtggatt cgaaccaccg accaattggt
    48001 taacagccaa ctgctctacc gctgagctac accatcaatt cggcactgcc agtatttatt
    48061 actcaacagt gccaatcgcg gcggtgttag tttctgccag gaaacgcacc gctacttgca
    48121 cttttcgtta gtgtcagacg ctttcgactt ctctcaatag aaggcaaaat ctttaagtag
    48181 atgatgtttc tgaggacagc acctactttg taatttatac aatgtgatgt atggaatcat
    48241 ttgttgtgga aaagtggtta aacctacaca acaatcctgt cttcacaacg ttgaggccac
    48301 tactccgatt gaatgtttgc ccaacatgtt acatatcaac gctcgccggt atctctgtga
    48361 atagaacctt agaattgata aaaatgtaat ggcctcaacg ttgtgtgctg gctaaccata
    48421 ccagccgggc tacgtcgccg ctttttaacc cagtataaac gacataattg aacaaaatga
    48481 cgtaacagaa tgggcggtca gtggctaaga atccgggagt catatggagt tgaaaatata
    48541 ccgaccgccc attctgttac ttcatcggag ggaacatgag tgttccctcc tgcgttctgc
    48601 aatcacactc gctcagtgtg tcccatttcg gtaacgaggc tggaaactga cctcgctggt
    48661 gtttggctta ttaggctact gccagataga cttcttcgtt tgcacttgta tttaagttca
    48721 aacagtcgca tctcaacgaa aacaaagtca tcttatacac aaaggataag taagtaaata
    48781 cttatcgata tgtttttatt cggttgtgac ttttttgatc aaggccactc tacgactagg
    48841 tgttcttgtg atggtggcag tgaatcgttt cgcttggata gtaatggttt cgttctcctt
    48901 aagttgcccg tagcgagttt ccaccagggt gccaagacgc cacagaccat catcgatacg
    48961 tttatgacta gcaaatttaa tcagcaacag ttttacgact aactgaccta cgtaaaaggc
    49021 gaatgcgact ccgaccgcta agaaataggt ggccaaccac cagtcccaag aagttaaatt
    49081 gctcattttc taacctttgt ttcatgaact acccgataca cacgctttcc gatgcacagt
    49141 gttttggttt taagttcctg cttaattaaa tcacgacaga tgccgaagcc gataaggaag
    49201 ccaccaataa aagctaatga gatgtatgga atcattttaa cactcctgat tctactaatt
    49261 gctgtagcaa tactttccct ttatcagtta actggtagtt ggctgagcac cacgtttccg
    49321 aaatgtttgc aaccaaccct aaacgctcta aattagctcg cgttttaggc ttccagtact
    49381 gagggaactc cggccactta cttatttcat gaagcgtttc tttctcccgt ttacttaata
    49441 cgatcattct ttatctccga ttcgatcttc ggtatcgcgt aagcaacgcg gccatttcag
    49501 tcgtgggtgg cgtaagctgc catcaggtgt tttctcgtga caatgaacct cgacaatacg
    49561 accacggtac ttctctttgt tgttccagat ctcgtccaga tatttatgct taatgccgct
    49621 cgcacgaacg atgacgccgt tttcaagacg aataactatt tttccaagcg tgttggcaaa
    49681 accagagtcc gggtcgcccg gctcaaaatc gataatttca ccatcttctg aatcttcgtc
    49741 ttttaacttc caccagctgc gggtacgttt aaactcgtaa acagagttcg gatctttgcc
    49801 catctcccct tcttcgttat cgtccaggcg tttcatgaag cgttcgatga agtcttcgtg
    49861 gctatggatg atatagaacg gatgcaggtg aatgtcttgt gtgtagcctt cactgcgatc
    49921 gtttttaaac aacgccacca gcatagccag gcgctctttc agcttcatac ctgtctttgc
    49981 atactctttg gatttagcct gtgctcgcca ttccggtagg aagaagtcga aaacatgata
    50041 aatagcgcca attgcctgca cgttcttttt gcgcaatgca gatacggact ggttaaatga
    50101 accggcagtt ccttcaccat cgaagaagat gtgtttgtgg cctgaaagtt tgcctaattc
    50161 tagcattgct ggctttaggt gatcgagaga cgtgattgga ttaccagtac gagaaaggaa
    50221 gttaacctct tcctcgtcaa caataacctc gcaaattact cgcaaaccat cgagcttaag
    50281 actgccaatc atcggccatt ttgctttagg atttggcttg aaggggtatt tgtcgccttt
    50341 ttctttatac ggtgacgcca gctgcacctc gaatttcgga atgggatttt caaaaacctt
    50401 gttgcatagg ctaatcccaa cacctgcttt cgggtctttt aagaggaaac gacggaacac
    50461 gtcctgccca tcggcgcaca ttgacgccac gatagactca acagctgcta ttgctgcgtt
    50521 ccccgttagc tcgcgagagg ccagcttatt cagtacatca atggcttttt cgtcacttgg
    50581 gatggactca ctaagcggct ctgccacttt gtatttcttt accccaaaac ggatgaaagg
    50641 gttgagcatc aatgaaacca tgctttgctc gaagtcatcc atgttggcca atgcctcttt
    50701 ttttgcattg gttcccatag ctttcatttc gtccagctta tgcttaagtg cgatcaactt
    50761 tttcattgtt gttttacctc catatgttga tcgatttgct catgtgtttc ttttgttgtt
    50821 tcttcaagca acgccgcata cacgtcagtg atcgctgttt gtgatgtggt gctcttctta
    50881 accatgctat taatcgttac actgtcacgt tttcgtttta aagttctggc ttgtttattc
    50941 cgttcttcaa cctccttaat gagcgcggtc atatcttcga aatacagaga ttctccttta
    51001 cgaatctctt caaccatcat ttttaacgcc ttgcatttgc ctgctttgat agcggttgcg
    51061 cacgactgga atgaagttcg aggaagacgg ttttctttga aggcaagaat ggtgtgctgg
    51121 caaacggagt agctgcaata tgccgactca ccgtttatct ttacttcctc acaacgaagt
    51181 gaataaccat tatttcctga aatagagggg atttttgaca aatcagcttt cacaactacc
    51241 gccataaata atcttgtact tacctatcgt tatttgcgta aaaaacgacg cgcggagaga
    51301 ttaccaactt ccccagctaa tcatcttgcg ctgattgctt tctaagcgat agggggcaag
    51361 aagctctgtg acatgattgg tggcgtatga tttagcctct tgctctatca tcggtaattc
    51421 gttagcaatc ctgaccacct tccaagcaaa ttccgccatt acgccatcac atgccttccc
    51481 agcatcaata acgatatgca ccagatccaa ctggcttttg cacatgtcgc aaagagacgc
    51541 atactcactc tctctgatta catttacggc atcattagcc cctttattaa gcaagacttc
    51601 caacagattt ttcggggtaa cgaaatctgc tttcagagat aatgtctttt caccacttat
    51661 cgaacgcaag tactcttcgt agttattctt catcaaaccg ctgctgcgca ttgctagaac
    51721 tgcttcttga atggccgctt caatagtttc gtcgtttgaa agacaaaaaa cggtatttgt
    51781 gtagatcact ctgccatcaa gccatgctcc aacctgcact ttaagactga gcgggttttc
    51841 tttgctaata aaaacaacta atgcagaacg tcgtgcacaa gaaggttcac cccatacgtg
    51901 taacttaacc ttcagataag gtagccccgg tagcgggatc tcgaccaaac gagtggcgat
    51961 atgatccatt gctgttttta ccgctttttc aatgatgtct agtcgattgc tctcgctgat
    52021 ttctaaacca gttttatcaa tgatgtcgca ggctaatttc tgaatttcgt ctttcatacc
    52081 aactcctagc taacagatga aagtattctc acagaaagat aagtaatcat ctacttattt
    52141 taaaggtgat tgttatgata gagccttaat gcccaaaacc ttgctttgca actccaactg
    52201 tttggtatac ggttttgccc gataataggc tttgataatt tgttctggcg ttgcaccgcc
    52261 cgggtcgagt ccttcctcac ccaaacacgc aactttgaca ttaagaccga tactggtcag
    52321 tcgcctggct gcggccatcg tgttgcgtat agcttgcttt tcactatccc acatcatgat
    52381 cacattacgt aatccacgcg ccttgagcgt caggaacgcg cccaactgat cttctgcatc
    52441 ctgagtggtg ttgccagata gatgcatccc gaacgttcct attggctcca cgtaatcacg
    52501 taatgtttct tcgtcgaaaa tagctcgttt gacccccata acatcgaacg caccctcaca
    52561 gacaactaca gtctgtttgc cgactgcatt atggccgttg tagagaaatt tacctgaagc
    52621 tggaagctgc ataggaaaga ggtagcggcg ttctgctgta ccggtaatgt ctcgtccctg
    52681 aaatgtcttc attaccccat ccagatcgta aaccggtatc agtattcgca tatcgaatat
    52741 ctgccctttt acctgatctg tatacggatc tacataagcg tgcttgcctt cgacgcagta
    52801 tcgtaggtca aaatactttg ccatttcagg tgatatgttt cgttcaacca gataatctgg
    52861 aagacggcca tctataggaa gctcgtaatg acgagggagt gcaactggcc cctctaactc
    52921 gactgtgctt gcaagcacta gctcttcttt ctttggtgcc cacccctggg aaagaagagc
    52981 gttctgcaca tattcctcga actgtcgtcg tgatttaccg ctgtagtgct tgaggaagac
    53041 cagcttattg aactgaatct cttcgggatg atcaccagcg aagcatttgc cgacgccact
    53101 ggtcagattg aaatatacct tccagttgga gctgccgcat accggacact ccttgatatt
    53161 cacttcacga ccgcgagtac tcacgcctcc acgtcgataa acgatacctt cagtatccaa
    53221 ccattgttca aaatctaatt cggcaattag ctctttcagc tcgctcacga taattccact
    53281 tttaacaggc aatattgtga ccaatctaaa tgttgatata acataaaggc tcatgtgttt
    53341 ttcttttgtg gtttggcaaa agaaaagttg tttcaccaat gaatcaagcg tggaggtgtt
    53401 ctccacgctt attttttagg taacgtctaa gattcgctca atgaagcgca tttgttcgag
    53461 gttttgttta acgcgaatgc tgatccctcc ctgctggtta cgtgaaccag caaagtagag
    53521 acgagcctct cctttcgctt cttcttcttc ggttttgttg atcgttatta ccaggtcagc
    53581 aatacgtact ttttcgatgt tgtcggcagc gtgcatcatt gtggcaactt ctgacgcgcc
    53641 accttcacgg tttgtctgcg atgccgtgat cccagcaacg ttatgcttgt cataaagagc
    53701 acgtaaatcg gtgtagatac tacgaatgtt ggcgcgatca tcacgaaggt cataactggc
    53761 acgcatcaaa tctgcgtagt cgacaacaac catgtcaggc accatgccgt tggctttcat
    53821 gctgttaagc atacggtcca gatctgccgg tgacatactt cctgacggac gctcaaccac
    53881 ccacaaacta ccaatcccct tcgtggctcc caactctgcc aacttacgat gaacctcatc
    53941 gcgccgttcc accagcttgg acatttctgt ctccgacaat cttgcatcaa aacggtcgga
    54001 taaaatggtg gtgtgaacct ccagcgagag atacagaaca ttgtagccag caagcgttgc
    54061 gtttatggaa aactcaccca ttgcggtcga tttaccggat ttagcgaacc ccatgaagag
    54121 caccatttca cgcttcgccc agcctttttg gtacagcaac ctatcgagca gagggagtcc
    54181 agttgtaatg ctgtttggca catactcctc tgaagcctca tattcacgcg ctttcaatcg
    54241 ctcacttgcg gaggtgtagt agtcatagat tccggtcgct tcgttcgatc caatctgctg
    54301 aaccttagcc atgattgcca tcgctccctg aaagtcgcct ttctctttca gttcagcagc
    54361 cttaatcaga gcatcatcaa acgctacact ttttgcgaag gttgatacct ggtcaaccat
    54421 gtacgaggta tcggacaatt tttctgcaag aatgcgctta aacgcagcaa caacatcggc
    54481 gaatagttcc tcacggatag tcttatcgcg tttcgcacgc ttaagcatat ccagaattgc
    54541 agatgaagag ggcgcgctct tgtacatacg gtaatagccc gaaaccatat taaccaatat
    54601 ggcattggcc gcattggcaa attggctagg cacaaccaga tctcccgcac gagtaagaaa
    54661 ctcgtgatca cgacaaaaat atgccgtcag tctgttctgg aaatcttcat caaactcttc
    54721 ggacagcccg cgtcctgtat ggcaaagttc tgtcatgtgc tttcctttgt tttttaaaca
    54781 aattgttttc tagtattagt taattagata ggggatcaat aaaccgccgt gcttcttcca
    54841 gttcttctgg aaagtgggcg gaaataaggc gctctggaac gatttccatt agccagatag
    54901 cggagaaaat tgcgcgtatg cgcttgctgc gagggatggt gcgcaaacac tccagaatcc
    54961 actcaaaata gctttcctga atcgggttga actgcatgtc tcccatatgc ttaaagctaa
    55021 caagagagtc atccagacgg gttgttgcgc gtctggctaa tttctcttcg aatatctcaa
    55081 ccagctctgg ctgccacaaa tgctgtgggc gcggcagctt gtcccacagc cgtcgtgcag
    55141 ctgcggaaag aacggtggag ataaagtagt cgtatgagca gcaatagcgg tcagcaaact
    55201 ggcgtgcttt ccatagagac gttttattgg cagtcgacaa ctcctgataa ggcaggcgtt
    55261 ttaacccggt ggtgaacgga gctgtttcaa agtgttcgcg accgtgcgtc agcatgatat
    55321 ttgagtactg acgtttgtat gcctccgtaa acagacaggt ggccataaga ggatgcatgt
    55381 ctcggtaatc aaaccacttc gtctcgaaga gttcagcctc gtctttacag cgcgacaaac
    55441 caatgttttc agcgacccac ttgtccataa cagcggtatt ccactctgtc atgaagtcgt
    55501 actggtcgtt gtcgatggta tcgaaaaaga tttggctcat gtggctcacc tggtaggtag
    55561 ttacttactt atcacaatgg acggatgata gcgactggtg tcagtttttg gaagtgaaaa
    55621 cggaagggag ttgttctggt ggtgtctttt aaaagacctg cttccgtata tatttaataa
    55681 gttacttatt atttatatac agaaacaggc tcctaatgga taccaagaac aacataattt
    55741 gactccaact ccagatgcta tgtcattgac agatttatca agggagatca tgttagaatt
    55801 agtgttttag cctcgtggat attgtcacca tgcgcatgaa tagacttcca ggatatggcc
    55861 ttcccgaact ggctttttgg cctcagccta aatatgaacg gaataaatgg tcgatgttct
    55921 gtctgaaact ccgtaatgat ggaacccttg cttggtatcg gcgatatgtt gatcgtggta
    55981 tgccaaacca cgctgttgat gacgactacg ataactatcc tgaagcaaga aaagcggcat
    56041 tagagctgaa taaaaacgtg agctttgaca ttgaaaaact tcctctatct atgtttcaaa
    56101 agaagtcatt gcgtttgaaa gttgacaaag cactcaaagc caaatcacgt ctcatggatg
    56161 aagagtatat aatgctcaac gaagcaatta aggcacacgc caacgatccg cgagtgacat
    56221 taaatgagtt aataattaac ccagattacg aaactttgcg tcaacccctt tttgacgtgc
    56281 taagcgagat gccttactta cattttgttt ttcttcccaa attccaagtt tcacttcaac
    56341 tcgttgctcc taacacttgg gaatgttcat ataacaacaa agctaagaca gcgaagatct
    56401 gctaccaaga acgcatagcc agaggttttg gtttatcagg tgctgcacat tggggaaaaa
    56461 caaaagcgac tattcgctca atgcttctgc cgcgtgctaa ccggttactg caactcgcca
    56521 gcgtaaaaag aatgctggat gaagctctca ggaatgggca aaaggtactg gttgttggca
    56581 actttgtttt ctggtttgag gacaaaaatc aggtaggttg gagtgttaaa gttgctagcg
    56641 aaagcgaaac cacctcaaga ggcaatacgc tctggaaaga aggaactatc atttcaaaaa
    56701 atcacggtcg aattgtcgtg ctgccgtata caaaagaaaa cggtaaacat gtaaaaggtt
    56761 ataccaagaa tgcccctaac gacggcaatg caattccgag acataaggat gagtatgttg
    56821 agttgccctt tgaaattttg gatggtgact tgatgttcag tctgttcggt gagcttaact
    56881 acgaatagct ttcatagaac gaaatatttc taaggctgtc caatgcagac cagccttaga
    56941 gatttatatc aacctttctt cattaactcg cgtttaattt catcggtacg catcgtgaca
    57001 tcggcagcag tgatcgcctc gttaagcttc acgatgtcct cgatttcctg cggtgacttc
    57061 tctgccagat ggaaaatagc agcacgaatc acatcagagc gggtgaattt ctcgaagcga
    57121 gggatgaact tcatcatctc cagcagttcg aagtattcgt cctccagtga cattgtgcgg
    57181 cttttaattt tctctttgcc acgagtcggg cgtccctgtg gtctgactgg ttggcgcaaa
    57241 ggagttgtgt tcttagccgg tgcatcagac tctttgcgct ttgctaggtc acccattttc
    57301 atggacatta ttcttcttcc tccagactca acagataatc tacaaattct tcaaactcgg
    57361 cttccgcctt tttgtcgcgc tcgctaccgg tcatttcaaa gatagaacga ccagactctt
    57421 ccgcatcatc atagacgttg cggttatata gattgactgg cgcagactcg atgccaaacg
    57481 tctcaacaat ctctttagcc gccagaatgc gagacacttg tgatggcaaa gccgggcact
    57541 ggttcatgac cgcgcggacc ttcactttat cgtttacatt gcgaacattg tcgataatag
    57601 gatcgatgtc acgtaaagat ttcaaatcac gacgcttagg acgcagcggg ataatgataa
    57661 cgtcagccat cagcatcgct tgtcgctgaa tttcggagtc gaagccacca gcatctacca
    57721 ctacaaactc agctctaccc tgaagcgatt ttaggtgctt aatgatgtca tcctgaacgt
    57781 atgcaaaagg aatcagctca aggtcttcgt tctgtcgacg gtcttcacac cagctcgttg
    57841 tcgtgcgctg aatatctata tcggtaatat aaaccttctt cttctttttg actttcaggc
    57901 aaacggcaat ttgctgggca acggtggatt tgccaggccc gccctttgtg ccgccaacca
    57961 caaagatctt ggtcattgga gagtttcctt tgcgtctatt attattgtct gaaacaactt
    58021 gttttcttat atgtgatata gcctaaatgc ctacggctgc tgtgtaaagg tttaatgata
    58081 ggtacataga tttggtaagg acttaataac aaaaaacccg ccgtagcggg tttaggaatt
    58141 agtggttggt agacctggca tcaaatgttg taacgcccac ttcaatgtgg cggtcttctt
    58201 tggagatgat ggtgagaatc tgactagatg ggtctatgct gacagccata gggttccagt
    58261 gacgtaactg agcctcgctg ttcaatagca gcgcatccca cggtttgacg ccattctgcc
    58321 caataccacc ttcaacaagg acatctataa attctttatt taaattgtca tagatttgga
    58381 cacgaccgac caaaagcata cctacagccc aaattctggc accgtaaacc gtctgatggt
    58441 catcacatac tacatggtca tgccaattga gaagaaaatt gtaaactctt ctagcaagtc
    58501 ccttttgttc ctttgaaggg gttacaaatg cccctttaat ctggacccca aggtgagagc
    58561 ctttttgtga gcgaaactgg aatcgtttgt agacgaacct ggcgatctga atgccgtcat
    58621 catcttcttg ctcatcacca actaattcac gaacactatg aactatctct tcttctgaat
    58681 gcccctgctc gtcaagatcc attgcatgtt cttgaagcca atctgcatca gggtcgccta
    58741 caaatgacac tgtcaaacag taatcataat tagacccgat caaggaccac tctgtgagcc
    58801 gtaaaacaga aaaatcagag ttaaccatgt agccaactct gattttctct cttgcttcac
    58861 attcagggaa atcagacagt acttttgaat actctgctat gaatgatggt gacatttagt
    58921 tagcaagccg catatccgta gatgaatcca gtttcgctat tttaacatct acgcggtatt
    58981 tatctttgat aaatttagct attttgcttt caatctcata gaaagcatcc tgttcacaac
    59041 gcacaacatc gggaaactgt ttttctggct taacaagaaa acgtaaccga ttgttgagca
    59101 ccatcatttc tgcaaacaat ttgatggtaa acttgccttc ttcaacgtca cttttaaggt
    59161 taacgatgaa acgctgatcc ttaagcatac ccggtttgtt catttcacga atttggagcg
    59221 agtcattcca atgccgcaac ggaaccgatg cgatcatctt ggtacagtct cttatcgtct
    59281 gaatagaacg agatgcgtta agcgcagtat gcatgtttcc accttagccg gtaataactt
    59341 tatgtcatag cggcgacctt gtcggtttag aggctacgag tatgcgtcga tttattaaaa
    59401 caaggtatga tttacttgac atccatgctt caaatctacc ttgtgctcta ataatatgca
    59461 aattttatgt tagaaaattc cgcttttatt atggcacttt tctgccccat ccacacaatc
    59521 gctcccctat catattgtga aaaagtactt ccctttcagt ctcttcggtc atgagatcat
    59581 cacggctgat gtatagcggt gtagccgcat cgcagaaaag cacaccagag gattgaggct
    59641 taatcacgca accacttatt gtgcaactca tgatgaacag tagaagcatc tttgcgccgt
    59701 aactcatcaa gtgtttcatt tttgacatcc actgtgcttt gaagtcgttt cctgtcttcc
    59761 tgttttgcct tctcttccat tgctcgtcgc gccgcatttc cgcccatcgt gtaagcgccg
    59821 accagaacga aaagaacggc agccagagta atcaaagcaa cttttagctt tatcatcagg
    59881 ctacctagca tattaggcca tccctttctg gtgttttctt acctgcgacc aggcaatgaa
    59941 tcctgccaca acaatagtgg caataccgaa gatgatgcgt actgtatccc cactagagat
    60001 atgaccttgt gctttatcca tagcagcgga aacctgcggc ataacatcgg ccagctgcgc
    60061 cagaccaata cctgctgtaa cagttgcgcc tgctgtttct ttagttacag gaacagcctt
    60121 cacggttttc accggcttaa cgactccagc tcgacgcaga ccttcctcaa taacttctgc
    60181 cgcataccag gtgttcagcg tttttagcgg acctcgccca ttctcatggc gaatgattgc
    60241 ctcaaccaaa ggtcgaagga tgtcgtaatc atgcagatcg atgatcatgt ctgcggttac
    60301 accaacagct ttagacacct cattaatgta ggtgtcagtg ttgttttcat tcggcggtgc
    60361 ccaacgttca ataacttcgc gaatggtatc aatacttgag ccgtctttgg cgcgacgttt
    60421 atcgtggtag gtaattagag tcactgccag agcacgaata ccccaaacag ggtctttaaa
    60481 cgtgcaaaag cgaggttcgt ctggattcgc aaccagacct tgccacggtg atcctttatc
    60541 aagattaccg gggttattat tacgaatgcc tctcggagtc ttcatccttg ttctcctgtt
    60601 attgcagtcc attttttacg ccatatgcgg ctaatcctaa aattagtgcg gttataataa
    60661 acgacgtcac tttagaaata atgcctccaa agaagccgct tgagatcgaa tctaacctat
    60721 taagtagttt atccagattg gaatgttgaa tactgtgttg agccggagtc atgtcaccaa
    60781 aatagttttt caactgctca tgaacctcat gaactatctc ctctctgatt tcttttccta
    60841 atttaccaac aacttctctg gcgacaattt tagctattct ctcaacctga tcatgagtga
    60901 cgcctcctac gtcattagcc atgcttcctc catgagcaat ctattttaat atccccttac
    60961 ataattttat gtaatttaaa tatcaactga aatttaaata atatataacc ttttatattt
    61021 taactaattt tcaaatcttg tgtaaccccc gtccttttca ttgccgttat aaaggccgaa
    61081 ctaggtgttc cttgccagta atagtgaaaa ccgttaataa cactattgta agtaaaaact
    61141 tgtgagactc caccaactgt tactgtaata ttgcttgccc ttccccctgc gatgtataaa
    61201 cccccagtgg gcggtaaacc tggaacgtag tgagcttgcg taagcacgat ccccactaaa
    61261 cgacctaaac cacagctcga agcaccagca attgtacctt ggttcttttc agccaatggc
    61321 caatctgaac ctacagcccc ccagttttcg actttatcag gaggattccc tttatagtaa
    61381 gcggttgcaa ttttacagcc catagatcgt cccaccattt gactcatcat aaaaggttta
    61441 cccaatccta acttagtacc cgcagcgctc atccattgtt cgcccgtttc gctcttagcc
    61501 gaagaaccga cccatcccga cgctaccccc attttacccc tccttttgaa tgcgtggggt
    61561 gagtatcacc cccacaattt aaattcagta gaaaccagaa actccaacag ctgtcgttta
    61621 cttctcccga agttctttaa tctcatgacg aagtgtttta aagccctcga ctaacaatgc
    61681 aattatgccg ttgtagttaa gacacagacg tttttcacca gatataacgt ctgtgtcttc
    61741 agttaccagt tcagacaatg ctttttgtgc atcctgagca attaaaccaa ctgacgtttt
    61801 ccaactatca tcagagagtt ggatttcata aagataacca gtaagtgcct ccaaacgatc
    61861 taacgcatta tctagcttta ccagattacg cttgttacgc ttatctgagc ggatctgaac
    61921 atcattaaat gagccatttc catttacagt caaattacct gttatcgtac ccccaccgat
    61981 aggcaaagca ccaatttgag cggcacttat actaatattt tttgttccgt cgaaacttac
    62041 tccatttata gtacgtgctg tttgtagctt agttgctgat gctgcgttac cagatgtatt
    62101 ctgatttcct gacgtattca cacctggaag attaatgttt gcgctaccat caaatgatac
    62161 tccacctatt gttcttgatg tttttaattt agtagctgtt gcagcattac ctgatgtgtt
    62221 ctgatttcca gctttgttaa cgcctggtaa atctatattc gctgtaccat caaacgatac
    62281 cccccctata tttctcgctg ttttcagttt cgtagccgaa actgcatttc catttgaggc
    62341 aagggcgcct atctctgaag ccgttggttt attttgagaa ctgtaattac gacgccatgt
    62401 tgggctatag ttatcgccgt tgtatgtata aataaactca ctattaagaa cagcaccaga
    62461 tgcacctcca gatgttgaag gtgttgtaat gcgtatagtg taattacttg taccggttga
    62521 aaacacctca ataacagaac cagcaagagg tataactcca catcccgttg atgcatttcc
    62581 tatataacag ttactaccat aagaccagct acaacgtgtg ctccactgag ttgcatcaaa
    62641 tgccccttgt gaatttaacc atgcgacaaa ttgtgcagtt gtccactctt gagtattccc
    62701 cgtagcaaga ttagcaacat attttgctgc acctagttgt tttggcgtga tgcttatatt
    62761 ctttgagcca tcaaaactga ccccatttat ttttcgtgcc gttgcaagtt ttgtcgccgt
    62821 tgcagcatta cctgacgtgt tctgatttcc agctttattg acaccaggta agtcaatatt
    62881 tgcagtgcca ttaaacgata ccccacctat tgttcttgcc gtttgcagtc tcgttgcgct
    62941 ccctgcatta cctgttgtac tctgatttcc aaccttgtta acgcctggta agtcaatatt
    63001 tgcagtgccg tcaaataaca ttccaccaat tgaacgaggt gttgctaact tagtggcaga
    63061 ggcagcgttt ccactagtac tctgagttcc tgttgagttt acacctggta gatttatatc
    63121 tgatgaacca tcaaacggta ctccgccaat ttttcgtgct gcttttaatt ttgttgcggt
    63181 atcggaattt ccgttaagtg agcctgcaag accattacta aatgtttgct ttgcactcca
    63241 tgtattggct gtactcaata aaggtatttt atcgccactt gttccatttt ccttcaatcc
    63301 aagatactga ataatcgcct gtacacttgc tttagaaaga atatctcgac caacagaagt
    63361 tattgttgct aatccagctg tatcattccc tgtaaaataa ggaagtttat tagcggctgt
    63421 tgttagtcca gccagtgcag tcagcgtagc atcaagaggt tgttttgtgg ccaacttgtt
    63481 cgtcattgtt gtggcaaaat cgggatcatc acccaaagcc cttgccaact cattaagagt
    63541 atcaagcgct tcaggtgatg atcctacaag acctgagatc gcttctctta cataagcagt
    63601 tgtggcaatt tgcgtattat tagtgccttg agtcgccgtt ggtgccgttg gtgtgccagt
    63661 aaatgctggg ctgttgattg ctgcttttct atcagcaaga tcctttacac ttttaaccgc
    63721 ttttgttgtt gcggccaatg attcagaggc gttatttgta tcgctactta attgaaccag
    63781 ccctttttgt gaagttgtcg cgtcatttgc agtatatttt gatttagcaa gatcaaacgc
    63841 agcctttact gcttttggtg ttgctgcaaa ttcttctgac gaactgctcg tctcactgct
    63901 taatttgaca atacccttca tagtcaaact ggcatcatgt aatgatatcg ctgacaaagc
    63961 tctttctgct ctagtagcag ctatctctgc acgctctgcc gatgcaagtg ctgatgtttg
    64021 gctttgcttt gcttcttctg ctttagccgc agattcagct gatttcgatg atgcgttact
    64081 agcactagtt gaggcttcag cagactttcg gctagcttca tccttcgaag ctctagcagc
    64141 agacgcataa gttgaagcct gattagcgga tgtcttcgca gaccttgccg actctctagc
    64201 ctgatttgca gaagttgatg cactgccagc accagaagca gcattcattt cagataattt
    64261 tgcagcctct ttagagacca acgcagcatt agccgaactt gtggcttctg ttgccttagt
    64321 ggaagctgtt gatgcagaaa ttgcagctgc gttcttcgcc tctaaagcac ttgtttctga
    64381 tgctttagca cttgatgcac ttttatttgc tgcattttta gacgctgtag catccaacgc
    64441 actattagat gcctgagtag ccttactgct tgctagattc gcactcttcg ccgcagccga
    64501 cgcactggcc gcagcctgtt ttgctttatc tcctgcggaa ttaattgcgt tagtattatt
    64561 ttcataccat tctacgtgct tattatgctc cgcgataatc ttagaaagag gttttacatt
    64621 aacattagtg ccatcttctc gctcaagagt aacttcatct acggcagtta accaatttcg
    64681 aactgttttt gcatcaccag acattcgtga taaaagagcc gtaaatctcg cactaaactg
    64741 tgtcaaatct ccttcgtagg tagtgatgat cctacatggt atatcacttt gcgtttcacc
    64801 agtatagttt tcaaccaacg ttaaggttgt gtcatttgtt acgcttttaa tttcatacaa
    64861 tttattatca ggtgcgacga caatcatgcc aggcaacaca ccattggccg taacgttcca
    64921 tgctgtccca gcaccaacca gagtattgct gccttgtgta aatgtgatag taccttccct
    64981 gtaccacatg ttgaatctgc tcctttaatc aggtgggcta tccttgccca ccattaagta
    65041 tgtaactacc tattattatc gataaaaata tttttttcca taatcaaagt cgaccaattc
    65101 ttaccctgag tacattattg tcgtcgtaaa cgtcgattct ctgcccattg ataactaatc
    65161 tcccatttcc tccactgtta ccgttaatct caagcgttcc gtttttgcca aatctccatc
    65221 cggctcgtcc tcttacgaag tttgttgatt gtaagtcacc tacctttgcg tttgtgatag
    65281 tgccatcttt gatataagca ccgttcatat acgcaacgct gttttcgata acaaatggcg
    65341 ttgtgatctt cccattaaca ttgttgacca aaccaaatcg atctgcccgc accaaaaact
    65401 gagaaagccc cgttgtatca atacccagcg caatgcccgc tacatacttc tgcccaccac
    65461 tcgttgaagt ttccatcttc aatgtgtacg aggttgatac ttttctatta gcgtcagcta
    65521 tagcagaagc ctgctgctga ataacggcaa catttccatc tacagacgct ttcagtgtat
    65581 caattcgttt tcctagagcg ttgtctgcac ttgttctggc ctttgactct gacgtaatcg
    65641 ctgcattgat ataatttgtt gtttgcgact ggagattagt tacccttgtc gaaagcgctg
    65701 catcagcatc agttctggct ttcgtctctt ctgctacagc agcctttata tctttacctg
    65761 tttgtgtctt tagtgagtta atctgcgacg tcaatgcggt atcagcggca gctcttgctt
    65821 gagtttcaac cgcaacagct gcacttatat ccttagcagt ttttgtttta aaacttgcca
    65881 agtcttttgc aatggcagaa tcctgacttg tacgagatgt cgattctcgc tctatcgagg
    65941 actgaagatt attagccctc gtagtaagag aagaaatttg tctggataat gcatcgttag
    66001 cgtcagttct tgctttggtt tcaactgcta ccgccgctgc aatgtcttta cctgtttttg
    66061 tttttaaact actaacttct cttgatagtg cactatcgcc tgatgctctg gcttctcttt
    66121 cctcattaat agcagctgat atgtcatcat ctacttttgc ctgcaattga ttaatttgtg
    66181 tggataacgc tgaatcgccg ctaactcgcg cttgttgctc acttttaata gcagcagata
    66241 tatcgttatt aacttttgtt tgaagttgag ttatttgcct tgataatgct tcatcttgcg
    66301 ttgctcttgt ttgttgctct tctctaatag cggttgaaat atcagtattt actttagact
    66361 ccagttgtgt gatctgcttt gaaagagtct cattggccga tgctcttgca gattgttcgg
    66421 tccttattgc ggcactgata tcaccttcaa atttactctg taattgtgtc acacgattgg
    66481 ctaacgcatt atcaccatca gttctggctg tcgcttcttc gagaatagat gctcgaatgt
    66541 tgtcaccaat ctcggctcgt atctcttcaa ccttagacgc cattgctgac acatcatcga
    66601 caagaatctt ttgagtcgtg gcaattttcg cgttatttac cagctgtctg tgctgatctt
    66661 cgtcctgccg caacgcaaga tcgatatttg tcttagaaat ggcttcaaga ttagttgtga
    66721 tttctgaact tactcttcct acctcagcaa ccgttctccg catttcttct acagccgcaa
    66781 cactttcatc aaccgatgat ctcatcgttt ctatcttttt agcattcgct agatctgctt
    66841 gtgcacgaac tttacgctct tcggcaatta aagatgatgc attttcaagt gccgcatttg
    66901 ctgcttctac gacaccagca acagaatcac cttgttttga tactgtatta cgtagttcaa
    66961 taagtgctgc atttgaattt tcaacttgtc gtaatgcatc atctactttt tcccgagtgc
    67021 tagaaacttc cgttctaagc tcgctttgcg cattctccag tgtctcgctg cgttcagtaa
    67081 acgatgtttt gaactcatcg atactactcg ttagctcctt atttaatgcg gaaatttcac
    67141 cttccagaga ttcgacgctc tgatcgatat gattattgat ttcatcaaca gcttgttgag
    67201 aagccttact gtcaatttcc tcatttaaag cctggccaag ttctgacgag gtaattttat
    67261 tggctaaaaa tgaaagaaca tctcgcgttg tggcctctgt accaagattt gagtttgggc
    67321 gactcaacat gccacgctta tttgctgatc taacccagta ataccaagtt tcactatccc
    67381 caagaccaga atgtgtaaag gtggtgcttg cagactctgc gatcagtttc gccgtatcca
    67441 gattgttggt ctgggatgcg taaacattaa tgtgatcaag gtctaccgaa tctggattaa
    67501 cccaattcag tatcacatta cgatagtctc caacggccgt taatgacgtt ggtgcatccg
    67561 gcggtgtcat tgtgcccagc acctgataaa cggtactgat aatctctgtt tttttaccgt
    67621 tgaatgaaac cgcatacagt tggaagtcgt agcgtccatt ctccgcgaca ttaacgattt
    67681 cgtattgctc ttcggttaca cgtgccgatt gccagttcga tacattgttt tcatcagaac
    67741 gtcgccaact gatccaatac tctggagatt tcccttccca tgttgcagtc agttttactg
    67801 acaggttgcc tgggcttgag agataagtcc cttcggtgat ttgcaaatta gacggcttgg
    67861 agtaagtcgg gtccaatacc gtcgtattct gcgggataag cgttgcacca ttgtcgattg
    67921 cctcatattt agacggattg ttctcaacag cggtgatgtc aaagctaccc gacgtttccc
    67981 cctgagcgat gttaacgatg cgaacgcgca taggttcgag atctggttct gtgattgtcc
    68041 atacaccgtt caaaacaggc gtttccgctg acgacagagc ttttgaaaac gtaacctttg
    68101 ttatgttttc gccagtttca agaacatcgc gttcaacgat tttgccttcc tgattcaata
    68161 tccgaataaa gctgccgctt ttagctaacg acacaggcgc atcgagagtg atgctgtttt
    68221 tggtaaactc cacaattcga ccggagttac gtttacctgc acgatatttg ttctgaatca
    68281 gaacggtttc accgggcatc agaaatgagg cgtctaaacc ggcagtaaat gtaattacat
    68341 ccgactccat tctggcggta tacaaaagcc acaaaccaac tcggtgagcc tggcctcggc
    68401 ttgtacatcc aaatgctacg acttctgttt tacgctcacc ataacggcgc attgcgtcct
    68461 gatcttcaac gtactcaatg ttttgcttat aaccgtcctc cttgttgttg taggttacga
    68521 gcgcaacgga tgggcgatct ttacgcgcag aacctttata ggtaaacaat ccatctttga
    68581 cgttcgagtt ggtaaacatc attactggat ctgatgggct atcctgcatg atgttaacca
    68641 tgccaccagc ccaaaacacc atgccgcgga atgcaccggc aatatcctga attagtcggt
    68701 atgcatcctg tctactggtg atctgcgtat tgattgcaaa gcgtttctct ttaccaccaa
    68761 agccatcatc aacctcttcg tcacaatatc gaccgatctg gtacagttgg ccgagatcaa
    68821 tcatagattc cgacacatac tgaccaagcc catatcgaga attggtaagc aagtcgaaga
    68881 gaatccacgc agggtttgaa gatgacaaca gcttaaaaga gccatcccat acgccatcat
    68941 aagtgttgct agcctcgttg tagtttgatg gtacgcgaat ttttaatccc cgcaccagat
    69001 aggaacgaga aggcatagag ccgccaaact gctcagaatt aaccttcagt cccaccaacg
    69061 cagagtttgg gtagttcatc ggtgtatcaa cgatctcacc gattgagtcc acccatgtat
    69121 cgttataaag atactgaccg ctgttatcat cagtaatacg aactactcga acactgtatg
    69181 cccgacctgg cttgggtaac ttcagctcat aactacgata ataagcaccg gttttcttcg
    69241 ctgttaactt aataccaacg tttttttcac cttctgcgac cacatctaca aatgttgaat
    69301 ctccatttga gatctggaac ttatactcaa cggtagtgcc gtttgtatca cccgtttttt
    69361 ggtctattct tcgcagagaa gggaacttca tgatgactcg aacacgatcg gcctcatcgt
    69421 tatctattga aaccgtcaca taatgcgatt tctttagttg gatattgatc gatttaggtg
    69481 tttcgacgaa atcaaagcca gacattggtg tctggtcttg cgagccgtct cgaaaatccc
    69541 atgaaattcc gctaaagttt gaagagccgt cttcatttac tatgggcaaa tcgtcgacaa
    69601 aaatagatct tgcgccattt accaagccgc caattacgcc ttctccaaga agatcgagaa
    69661 tagaggccat agcacgagaa tttacggtat cgtctgcttc aactggtgtg cgactagagc
    69721 ttttgctact ttttttgcca ccagaacctg caattaatag aggtaacttt ttcttcttga
    69781 actgttccat gtccaaaaat ccttgattac ataagctggt cgatggtgat agaagaactc
    69841 acgacctgtg agccaactaa aatttcctcg ccatagataa gctgtacagg gttcccctgg
    69901 tttgttgtgt tttgaggtcc atcaaagtaa taagagttcg agttatccgc ctgcctcaca
    69961 ctttcgttag tggcttgcgg agatatgatt tgcgctatac cgcccatcat cagtgacaaa
    70021 ccgagagggg caagagcggg catcattacc gccgatacaa ccaacaaagc ggcccctact
    70081 accgtctgaa accacccaaa agcagatccg ccgcttcctc gcggaacagg ggtaatgcgg
    70141 attttggcaa tgttgtcaga ctgccccatc atctgatatt cactctcgtc cacagaccac
    70201 ttgtggccct gtttattggt gatctggatg tggtatctgt cataggtttt gatattgcgc
    70261 ttcatccatg ctttaaaacc aggcttattg gcctcaatta aatccagagc ctgttttgta
    70321 ttgcgcacct ttagatgcca gtggcggcca aaatgtttgg ccatagggcc gccaagctgc
    70381 acatgaacta actcagacac gtctcatctc ccttgagcaa gtctctgtga cgcaagtgat
    70441 gcgtcgtatg tttctgatac atcccgccgt aataagcacg acaactaagg cggtcgatct
    70501 ggtgatgaag aatcattcca tcgccgatat aaaccgcaca gtggtcaggc atttttccgt
    70561 attggataaa gaagacgtcc ccacgttgag gttctgttcc gggcgcaagc cgtaccaatc
    70621 cttcgtttcg gtagttctgg tcgagaatgt cgttatcgcc ggtgtaccac gatggaatat
    70681 gcaggtgtgc gttcgggttt agttcgacgt taaactcacg cttcaaatag tcccgacaca
    70741 acatccagca atcgaatacg ccaaatacat acggtctgca caggtatggc atttcgaaac
    70801 catcaggtgt gatcacattc atctcgctaa aatggaaagg ggcatctccc tcaacattct
    70861 tgcgaatagc cagaatcatc cacggaactt ccgtcgcttc gcagcctgca cgatcggcat
    70921 cagatgcttc tgctgattca tcagtatgtg aatgccagat tgcgataaca tcacccgcat
    70981 cctcagccgc cataatgtcg tcaacgtgca ttacaaaagt gttctgcggg ttctccgaaa
    71041 cattccgcgc ttccataaag cgatatttgt cgccattagt tctaaccaga aagccacacg
    71101 cttcattagg gtagcgattt atggcgcaga gatagatttg ctgcataacg tcagagccaa
    71161 gctcagggat tgctttatta cccataccgc gtagctccaa taaatccacc aaaatggatc
    71221 acaccgtcgg caaaataatt ccgacgcgca ttacaggcgt cataacgttt tgtgcagtaa
    71281 tccgcaccag acatagacgt ctgctggtta tttttgtcga aatatggacc ggtatagccg
    71341 cattctggcc ctcggtattt ccacgggcag gtgtttttaa tgatctgacg atacggcagt
    71401 tgtaccccca tcaaatcgaa cacactggac aattcaaact cgacaacctg atgagtttcg
    71461 agagttttct gttcgataaa ccacatttca tccgggaaat gttggtttgg atctgctgtt
    71521 gggttgccgt ctttaaaatt aacggcatcg aggaagcgag ccagcgtcat cttgcgaata
    71581 atgcggcagc caacaagatc gtcgttcgcc tgaacttccg cagagacggt tccggcaaag
    71641 ttcgatacct gaatttttgg acgtggcaac gttccctggc cagttttgtc aaagcctgat
    71701 gctttgattg gccacggctc gtatgtcact ccttgccaga cgaccggttg catcagttcg
    71761 tttgttccgg cgtggaagaa tagcttcccc cctgaagttg tgttcgacat atccagtacg
    71821 aacaactcaa tgagtgcaga gggagataag ctctgaatat cagctttaat tcccattgtt
    71881 tcatccttga aataagtagg cgctgacatc ctgtcagcgc cacaatgata gtaaattagt
    71941 acttacttat ccagatactt aagcctcaaa tacttgtctg aatgtagcag ttaagacaca
    72001 gtacccctga tatctcttga ccgtatgact gtcacataca actacaatct gcttgcctct
    72061 tggattaacc caatagaacg attcaacacc tgatcgctca gtaaggaagt catcgattgc
    72121 attaatttcg ttgtatgatc tggtaaaggt taacgaccat tcttctttaa tacgattaag
    72181 accttgagcc tgtcgctgct cgtagtcatc accaaaatta agtaccgtta cattcggttt
    72241 tacgctcttt tcagattcgt aatctggata ccaattaaac gtttgtcttt ccatctcaca
    72301 tccttgtgag actgccccga ccggggcagt cgatagttag ttacgttgag tgtttgggtt
    72361 gagtgatccg ccagggcgtt tctcttgagc gatagtctca agcgcgattg ctttcatccg
    72421 ttgagcggca ttgttccata tgctttctgt atcgccggat tcagttgtgc taccgtcact
    72481 atggacgttg atctcaattg atacaggaga aagaacattt ccgcctccac tcataccatc
    72541 ggtactgagc gttacaggga ttgttcggcc atcaggcaat ggaacatacg cctcattcat
    72601 agaaccttcc ccaaacaacg ctaattgagg cgagttagca ataccgcctt tctggtatgc
    72661 ccggagcggg atcacgccgt cttttccgaa tattcctcca tttgcaaact tcgggattgc
    72721 aggaatgcct ttagttccgt cagccacact gctggttgcg gttttagcag agcttgatgt
    72781 caagctgtcg aaaccaccgc cagcccaagc tgaaacaaga ccagatgcaa ccgttgctcc
    72841 aaaactcaac cacttattac cagagccgga agcattagct ccaagcattg caaacgcggc
    72901 agacagcgcg ccggtaacag agctgaggtt ctgcatcgag aagatggagt ccttcacggc
    72961 ttttgtctcg gcatctttgg cttcagtgct atcaaatagc cctgataccc agctaccaat
    73021 cgcatttgtt gcagtgccaa ttgcgctggt ggtctgctgt gttgtttgcc ctaatccagt
    73081 taccgaactg gacgtctcct tcgtggcttc tcctaccgac ttgtcgccgt taacagtgtt
    73141 gcccattcgt accccttgat tggcaacggc ggaagcaacc ccggtcagca aattaccact
    73201 ctgtgaacta ccagctgcgg tggttcccat ccccaacatg ttcattagag gcagcgtgat
    73261 ttgcgacttc acgaccatat tggtgatatc tttcaaaatg gactgagata ggctggagaa
    73321 actcatcttc ccgttaataa cgaaatcagt caggacatca gttaagccac taaacaaatc
    73381 agtccaggtg ctttcgatct gctctgccag gttttcgtat tccagtgcca acttctgcgt
    73441 cgcagtcccc gtctctttaa taagcgcggt attgccagca gcaatcagtt gattgatttt
    73501 ctttgtataa agcgccacaa ctttaggatc agacgcctta tcacgaagtt ctatcaacgc
    73561 tttgagattg cggttgtagg tgtcttcgaa atcagcaact ttctcttcac gagacggcgt
    73621 atagccagca ctaataatgg aatccgactc cggtgcccag gtggagatca tctgctcaac
    73681 attgcggcga ttaaacatct cgcgatattc aggtgtcgca tttttgaggt cttcaagacg
    73741 ttttttcgcc ttgtcgatca tctcttgagt gatgaactcg ttaggaaccg cattagccaa
    73801 atctgtcagc gatttcgttg tatcgcgaag agactgatca aacgataccg tagccttaga
    73861 gctttcaccc atttgcccca taagctgatc ggctttatcc agagccttct ggtatccggc
    73921 tgccagtttc tgttgcgctg tctgttcttt cttggccgca cgctgcgagg cattagctga
    73981 tcgttgggct gctttctcgg cggctgcggc atcctgttca cgagctttag tcagtgcggc
    74041 aatggctgcg gcacgctctt catcgctcat tttctccaga gagctggcac tggatgcttt
    74101 ctgcaaatta agctgcgtct tgagttgttt aggcccaata atcggcttac cttcgaagtc
    74161 catcatcgga gtgccgtcag gcaaagtacg ttgataagtc gcagaatcca tctggtttcg
    74221 catatattgc gccagcgcct tctgagcagc tttatcagtt gtacctaacc caagaacagt
    74281 cccctggttt gacattacgc ccttaccagt tttggccgcg ttatctctct cgaactctgc
    74341 ctgtgtcagt tcctgagcaa cggcttccaa atgctcctga taaccacgga tactgccttg
    74401 cagtttctgg atttgctcgg tatttccatc ctttttggct ttttcaagct gatcattaag
    74461 agtcgctatt tgcttctcgg tagcattctt acgagaagaa aatgaatcaa ccagtttttg
    74521 cgcaggctcc agatagcttt tgtttaccgt ttcacgtaac ggtgccaata gcttgttctt
    74581 ttcgtcatct gaaagtgaac cgtcatcatt gattttctgg atcttatcca gagcctcctg
    74641 ccgggctttc acgaatgttg ccgcgaaaat ctggttttcc gctcgaattt tctcaatctg
    74701 agattcggca gcctctttag ccaaacgctt tgctacagcg ccgtcaccaa gagctatcgt
    74761 gccggttatc ttttgatact cttcctgatt ttttttcagg cgtgcttcga tgtcagcctt
    74821 cgactctttg tgagtaataa caccggcaga gttagatacg taattaacac cctcaccagt
    74881 ttttaatgct cgttgatcag caagaatctg cttttcgagc ttttctgcgc ggtcggccat
    74941 ttgtgcacgt ttggccgccg tcatcgcctc tggtattttc ctaatctcgt caacgacctt
    75001 tgaagtttcg ctgcggagca tggtcatgta cgtgattagg ccagcaacag ctacagtggc
    75061 aactgtaaat gctgccccta taggatttgc tgcaatgaac gccgttaatc cagcaaaagc
    75121 gcctttaagc cccgtaatcg ccccacggat ggcgaaaata agagagggga tcggagccag
    75181 ccccatacgt gccgcacgat tgaatcgagt tactgctgta gcgccgaggt taaatggagt
    75241 ctgtatggcg gtcgccatcg tggcaaaggt gctaaccatc ttgctgcctg cgccaactac
    75301 tcccatgatc cctgctcgca tcagtttgaa cgcaaccatc gcggccacga ccttaccgag
    75361 attaattacc agctcttggt tctttgctaa ccattgagca agctgacgca acccatcgat
    75421 tgccgtcgtt aaccccgaac ctaaagaatt ggcaaacgaa atcccttcgg cgctattcat
    75481 gattgaagcc agttctttca tccccttcga tagagaatcc agatatccgg cctgaccaac
    75541 ccgatcagca aataacgtga atgacgtctg aagttgcgcc agcgcacctg tgtaggtttg
    75601 catcatgtct ttcgctgcgt tctcattctc cgcacgcaga ccaacaaaca tcagagacaa
    75661 cgcctgtttt gcctcaaccg taccgctggc aacggcttta gtcagttccc ccatagtgat
    75721 gcctgcggcg tctgccattg cctgcatcgc gttaggaacg gcttcaccta attgctgacg
    75781 tagctcttcc attgacacaa cgcccttacc ggacatctgc tgaacggcca cagccgcacg
    75841 tttcaacagc tcactatcac caccaaaacg agcaacggag tccaccagcg ccttcagaga
    75901 accatcggtt ggatctaagc cagcagaacg aaacttcacg aaggagtctg ttaacgcctg
    75961 catcgcgaac ggcgcatttt gagccatgtc tacgatgtac ttcatatcat cagcggcagc
    76021 ctggcccggg ttggacttct ccttattcaa ccctcgaagc atcacccgca tacgttccat
    76081 ttcggccgca gcttcaataa taggcttctg ccaaccaaac atgatactgg tgacggttct
    76141 tgcagcatct ccgatctcac caagcaggaa aatgttgcca cgaaggccag agaacatact
    76201 gccttcgtta tttttaccga tatgccaaga gtaaccacca cgtccaccgc tacccccacc
    76261 atttccgctt cctgatgtgc gaacgcgtac aggcttgtta atgagttgct gacgtccgat
    76321 aacttcgtcc atctgctcac gaactttttt tagtccctcg gcagcctggc ttgttgtcac
    76381 accccaatta ctgagtcgct tcgtcgtggt gttaaggcgc gtattcatgc cactcacgga
    76441 cgcagaggct tctttgacct ccgtaccaaa acggcttgcg cttttgcttg cataggtcgc
    76501 ccagtcagag aaatcattta gctctgattg cactttacgt aatgacgcgg tgagtttatc
    76561 tactgaagaa gttgtcgtat cgacgcgctc aatcagagct ttaagaccag aattgaggct
    76621 ggtgatgttg ccacgcattt tacgcgtagc atctgaagca agctcaaaac cgacagctac
    76681 atcctgtagt ttatctgccg tagaatcgag cttgctttcc agaacgccaa tgatacgggc
    76741 gaccgaaccc aaagagcgtt caaaggtttg gattttttga gcaggctttg ttacctgctc
    76801 accaaatcga gtaagcagtt ttcccgcacg atcgattgac gctgtaaact gtttgtcttc
    76861 cagcgacagg ataaactcta cgttttgtga cattcccttg tcatcctctg ccaaatattt
    76921 gcatcagttg ctctttggcg tcagggtctg ccctatcctg gcttggatcg tagactttat
    76981 ctgttacgac tggtcttcca atcctgagtt gcaaaccctc catgaacgcc ttcacgccct
    77041 cgccatctgc ctgagcagcg cgagcgactt gcaggttgcg gacatcctct tccgcacgta
    77101 gacggtcgat gttgcgactg agcatccaga acatcgtgag agggatgttc agtagttcta
    77161 acggcgacac ggcgtagtga gcaactatac gactgaaata gaatccgaga tctattgaga
    77221 cggtccttgt cccggattca tcgcgggaaa ttactttgcc ccttcgccag ccgctttttc
    77281 gttttcttca tcaatcactt ccatagcgaa ggtgaagatc tgctgaagtt gcggaacagt
    77341 cagtttttca agaacttcgt cgggcactga agggataacc ttgcgaacca gatctgcata
    77401 agctgtcact tgctcaacag gagacatatt ggtcagatct ttaccttcca tctgcttaat
    77461 ggaaacgaac agacctaccg tcatttcaac gatgggatat tcctggccgc caaatttgac
    77521 acttttttta ggaggtagga tggagtcgag atcgagtaat tttgtcattt gttaaaatcc
    77581 ttttaaaaga gaggcccatc atgagcctca atttatagat taataagttg cttgattatt
    77641 gattaaccgt cactaatttg gttactttct tgctaccgct attgctggtg aatgatatgt
    77701 ttgcagaacc ttgttccaca ccacgcacaa gaccagtgtg atcaacagtg gcttttttat
    77761 tatcagatga ccatacgcct gttttatcaa ctacatcatc tggggtgaaa ttagcagtca
    77821 gttgaactgt cgccccgacc tttacactcg gagattcagg tgtaatcgtc acgtttttca
    77881 ccggttttgg gccactcatt ttgccaagaa ccccctcatc atcagggtac gcgttgaact
    77941 gaacagagaa cacacgtaca tcatcagact ggtaggacat agtgaagttg ccagcggttg
    78001 ccgctttcgg gagcgtcaac acatagtcgg tggtgtcctg cggtgtcagg acgagttcct
    78061 tcgccacgtt gatcagattt acgccctgtg cagatgtgat ggtgacggag ttgtcatctt
    78121 cgctcaaagt agaaccaggc ataagatcga ccatgttttt gagcacagac tcggccagag
    78181 gtgcggtaat ggtaatgtta cgcccctgaa ccaattcaga gattactgtc tgaccttgtt
    78241 gatcgactgt aactttaagc gtttcagtgg ctacttcaac ctgaacacca cctttggtgt
    78301 aacccagatc cacaccacca aatgatacct tgcaggcacc aagcttgatg ttttttacat
    78361 gggtattaga cattgatgga aactcctttt tccgttaatt tcacgcattc attgcgctat
    78421 atttaagtat atacttacct atttatttaa ttcaataaat agccagcaaa ttcaactgga
    78481 ataccggctt ctattaacga tccatcattt ttcggataaa cgacaggcat agccataggc
    78541 cgtacaagtc gaaaatagac ctctccagat tccgtttcct ctacgggaaa catatcaatg
    78601 atcttacgcg ctttctcaat agtcgttgta attgacgagc cacgcacaat gattgtgaac
    78661 gactcatgat aaaagccctg taactcatga tcgatactga taccggtatt tgggttaata
    78721 agcaggacac cggatttgac ataggcaggt aagtaatgac agaaaatgtc tgtccctacc
    78781 gtgccaatct ttgccttctg catcaaattt gcaaatgctt caataaacac attaacctct
    78841 cgtaaacccg gcttttttgg cagcctcaag aatcgcttcg gagaactgct tctcgctaat
    78901 ttccaccgct ctttccagaa agtgtggacc aacacgaggt tttacacctt caattggtgg
    78961 gtttgtcacg ttcttcattc gagaaagata accgagacgg tatttgccca gctccatgta
    79021 cttagcatag tcacctactt ctacgccagg atgcccctga cgtggctttg ctccagacac
    79081 agaaagctca atgcgcagcc ctgaataacc ttctttaatc accctagcaa agatggctgt
    79141 ctccagagat ccggtttcca gcggggccat tgcacggctg aggcgctcaa ccaaacgcgc
    79201 cagcttttcc atatcccgaa taagatatcg cttaaatgct ttctggctgt tgttgagtct
    79261 attccccgca cgtttgaact gatgcgcatc gtatttcaga cccatatatt cgcccctact
    79321 tcaagatgcc caggtcttcc tcgtagcccc cagcggcgat gaacactgga cacctttaat
    79381 ttttgacctt caaggatcag tacatcatca agttgtacag ccgattccaa agggacaact
    79441 aacacagcat caaacagctc cagactcgcc ttgccacgac taccagagct atcagcacga
    79501 actgacgatt tctcattact ctgttcgaat ttaactacac cgacattcgt cttcctgacg
    79561 aactgcaatt gcgcttcacc gtagacgttc tttgtgccaa agcggtagat cgcaatttct
    79621 gtttgccatg aaatattcac gctctctccc tgttgtcgat ctcattgcca gccaaaacta
    79681 ttgcttccaa aaccaaataa tgagaaagac attacgaacg tcgaacaatc atacgattgt
    79741 ctatataacg gatcagtaaa cgccaggtgc tgcgagcaac atgcacgttt gctgatttac
    79801 ctgtacgata catgttggtc gtttcaccga tcgattctga caaaatgcca tcctctcgtg
    79861 ctgcgacgat atcattgcca tttgctatct cacacgcttc attaacaacg gctagcatta
    79921 acgcttcttt gaagtagtca gggaactctt caaacttctc ctgagtcatc ttttcccaat
    79981 cgactaaatc atgccggtac gctccatctg ctccccacgg aatgtcatac acattcagca
    80041 tattttgagg gcgatcgtat cggtcaaagt cgatacgtag aattttgcgg attgagaacg
    80101 gtaaagtttt aatacgtctt gtagcctcaa tgagacgctt gcgcattaag ccttcaccat
    80161 ccgacagcaa agtgtcccca ttcagcatat cgatcgcctg catttgagca tcagcgacag
    80221 ttgcaaacga ctgttctggt atcgacagtt caaaagtatt cagcagaaca tacatttgac
    80281 gctcttcatg cgtaaggccc gatgcaacag ccttcacaat gacgtaccgc agatctcgtt
    80341 ctttctcaga gagctggtta tattcagccg acacgacaac cggaatcgac atttgaccgc
    80401 tggtaatttc tagcggctcg ccatcaacga gaatagcccc ggtgctgtcc tttactgtgt
    80461 aggtggcaga ttcgatatcc agcacgttga aggcaaatga aagagaaaca gcttcaccgc
    80521 tacgatacga gtcgatctgc gccattactc accgccttgt gctttcatga tgccgtcaat
    80581 catttcgaca attcctttcg ccttaacacc aatcttattc ccaattaaac gtaaaccggc
    80641 aataccctca ctatcagcaa ttgactccag ttcttcccgt gtgaaagttt gaattggttt
    80701 agctggttca tctgccgttc cgcgcttcat tggaacaatg tcaggtgctg atggctcaac
    80761 aatctgatct acgttcagat cattacgttc gccatatgca gcagatggag aaacgttttt
    80821 accctcaaca gttgatgcgc gcattgatgc acaaatcctc tgctggtcga taaaaggtaa
    80881 ctcggaaacg gataccccat tctcaaactt aactccacac aacattcccg aataatcaga
    80941 aaattgcggt tccaataaac gaatttttgc tggtttcatt acgctttctc gctatacagg
    81001 catatttaaa acctacaaaa agggtgggcg tatagcccac ccttaagcat aggtaattac
    81061 acacctatta ttttagttaa atttttacat tgttcagtgc agcgatagcc ttatcgtgct
    81121 tattcgccag agagcagtac catttcacac gagtacgtac tgcatcttta ttctgtaccg
    81181 tgccaatgtt ctccacaacg ataccagcat tgtcaccgcc atacagaccg gtaagaccat
    81241 tttcttctga caaatgcaga caataaatgt tagcagtgcc ttcctggcct tccgttttct
    81301 gctctgaatc ttcagtaact ttaaccccat ttttaggaat gaagtcgtta acaataaatg
    81361 gaataccatt atggcacagc ataggacgac cgaagttctc catcatgatt tcagacgggc
    81421 ctacgtttac tgtacgcagc agcgcacggt aagcacgcag gtgctctgaa cgcatcatga
    81481 tgcagtcagc gcccaaatct ttcaccgcat cgaccagctc gtcgaacata gagaaagtca
    81541 tagatgcacc agcaatgtcg atcttctgat ctttgtgcat cagacgcgga ataccatcaa
    81601 acgctttggt attagtttca gaattaccca agatcagatt tcgacggaat gcacgagcca
    81661 gacctttaac tttctggcga acctgaacag ccaactgatt gttagtatcg gacatagttg
    81721 ttgctaagaa tttatcaacg tcaacgtcac cagccagaat acgcagtttt gcaacatgtt
    81781 cgctgaatgt tgctgcgcct tccgcaatag tatcgtttac atcaataaag gtcgcttcac
    81841 taagcgttgc ttcgcgatta taaagatatg ccttcgagtt aatcttcata aaaggcagaa
    81901 tcgcaaacag gtcatcgcga tcgatgatag tttcgatcac accctgttca agttcattat
    81961 tggatagctt ttcagcttct tcacgcagta atggcatcta tcaattccct ttgatttaga
    82021 tgttacttaa gtccaatttt cccaagacca gaggtcaact tgtccatcgt cgacttagac
    82081 ttctgttgga ctaccttatg ggttggtttg ctattagaac cagcaccctg tttggcttcg
    82141 ctgcgcatga ctgcgtcagc ctctggatct gcccgcacaa tgcgttcaat cgcagattca
    82201 aatgacaacg tgtttccttc accgtcaacc agaacagctc gatctttgtg gcctgccggt
    82261 ttgtcataac ccactacgct accgtcatca ccgacttcaa aatgagaacc gtagatcacg
    82321 cgagctttcg cgggagtcat tagaactttg tcacgaagga aggcagaatt actaaatgac
    82381 gcgccaacgg tcatctcaac taattgagat ttcagtgatg cgttttcact ttccagtgct
    82441 gcataacgtt cgtcacgctg tgccagctct gcctggtgtg cttcgatcat ctgtttttta
    82501 acagcatcga actcaccacg acgctccagt tcagcttgct ccgcctcacg acgtgtgttt
    82561 tctgcggcct gttcagcctc taaaagctgg cgagcacgcg ccgggtcgat atcaccgtac
    82621 tgagcaagct gatcggccaa tgtgcgctct ttctctttgc gcttcatgtt ctccttcagc
    82681 agttcagcac cagctttctt ggttttacga agttcggcca gtaactcttc ctgagtcatg
    82741 ccagcgtatt cgtcatcgcc cttcggctgc tctttttgtt caccctgttt gtcaggatct
    82801 tgtgtactct gctcattatc agcagctaca ccgccagcgc ctccacgctc atgcgcttca
    82861 gcgacatcca tcagaccacg acgggccaaa agcatttgcc acagattcat aaaaattcct
    82921 tttgttactt atcactcgtt ctcttgagta gatgagtccc cattccctcg gggttgatct
    82981 tgcccgcttt cttgggctgc accacgatca taagtaagta ctgacttatt ttcaagggta
    83041 tttagatcat tttttggagg aaaattcaag agatctttat caaattcctt tttcatcgcc
    83101 tcagtaatgt tcgggaaaat cttctcaata agcatttcca tctggtgacg acgtacagag
    83161 tccggtgcct gaagtaatga cagtttctcg gcaacagaaa attcatcagt aaggccacga
    83221 atatcgaaac tttctggata cgcaattaaa gagtggtctt cgtccagatc tacccccatc
    83281 catttcgcaa ccagtagcat gatttggcgt tcagcccttt ccagacgctc tgcttttgtg
    83341 acaagcaagc tatttacacg ctggaagtca tacatttttg cggcaccaga tgaattatcg
    83401 attccctgtg cgttgtcctg cttcgttcgc tcaccagcta caccaactga atggtagatt
    83461 tcgttaatca ccgtcttaat cgtagtgatg atcatctgag cttgtttcgg gtctggtgac
    83521 agataaaacg gctggtttcc accttcagaa tcgtaggtga agactcgctt tgtgcccatt
    83581 tcaagcactt tagtgtggtt ttcatcacca ggtaaaagcg actgtaccgg tatagccaac
    83641 tggctaaatg tctgatcctg aataatggca tcaaggtttg acagatagtt tgcaaccgca
    83701 cgatcaagat aagcgatatc atcgatcaac gatgggctga aatacggtga ttcactttct
    83761 ccaatacaat caacaggaaa cacaggaact acgccgagat tatgctcacc gctatcctct
    83821 aaaataactt ttgcctgacg gcgacctgct ccaccagcgc ccttcttcac ttcctcacgg
    83881 aacagatacc actcgttttg tgtccacaga cgataacgtt ggtattcctg acctgtagaa
    83941 gtaaaaggat ctgcgtcatc acgagcgact tccacaatta acgcccacaa catattcccg
    84001 tcgtcgtccc atgccacatc cagcatttgc tgaggtgaaa tccagtaggc gtaggcgcga
    84061 gcatctttct ttttctcgtc agctactgac tcaacatcac cactcatcgt gctatcgaca
    84121 acaacccata tgcgaccgta aatagacgac tgcaaatcaa tagcggccat aaatgagtca
    84181 atagaggcat tctggcgagt cgcacgtttc cagaaattgc ggatctgctc tggtgcctct
    84241 tcgatatttc tatgaatgtc ttctttaaag agatatttgt tgatgaggtt taccacctca
    84301 cgagtgtggt tgaagcgata agcacgttca actcgctcct taaactcctg atctccctct
    84361 ttaaagtaac gaaagatatt gtctgtaaac caaccacgcc cgccagcgta agtgctggcg
    84421 aggaagtccc aatgttcttt tttcttttcg tattccgggt ggcgtcgcgc caccagatcc
    84481 ttaatttgtt tgtcgttcaa ttccatttga ataaccttag ataattactt acctatcgag
    84541 aaccaccaag aataacacga ttttttactg gatacctacg atgtactgga tagcccaatg
    84601 cgtctgcact atgctcaatg ccacctgtct tatccatatc tcgtgttcca ggcttgtaga
    84661 tgactttttc cagtgaatca atcaaatgct tgcacttcgg atcgatatac agccggatat
    84721 ctccagatgc ggacatcagc attcggttaa cagcattaac acgatccgca attggcgggt
    84781 gctttttcga ataatcgaca cgtaagaatc ccttctcttt gaatatgtct acgtcagact
    84841 ccccacgagc atgttggcga taagcacctg ccggatctgg aaataccgtt acctgtgatt
    84901 tccagcgcca gaagcgtctc tcaagctcat cacaaacttc tgccgtgtta gaggaaaaga
    84961 gcaccaattc atcaattgcc catagctcac catttggctg aggttgcaaa attactgaag
    85021 acatcgggtc aatgttgaag tcctgcccta cccatatagg caaccgaggg ttgaactgaa
    85081 gcggttttac atgcacgtta cgatcgaacg ggtagtaaac gcgcccggac atgttctcaa
    85141 agctcgccag gtactcctga gcgaacgatt tcgggtccat atcgttctta gcagcttcaa
    85201 tttccgccgt aggtacaaac ggagaatcgg cagttacaaa ctgccagctc ttccactgtc
    85261 cctttctttg cagctctacg ttctggccta tagtccacag tttgtgaaat tccgagaagc
    85321 cttttggcgt accaatgatc agtgcaccgc cgcgtgtcga tgaaagagta ggtcgtaaca
    85381 ccttgtacca ggtgtcagcc ttcatatcct gaaattcatc aagcactaca aaatgcaacg
    85441 ctacgccgcg cagagtgtca ggcttatcag cacctttgag ggcgatctcc gaaccgttct
    85501 tcaacacgat tgtcatcgtg gtgtcgttct ttttcctaac ccacttacga ggcagaactt
    85561 cctgtagatc gtcccacaaa atctggcgag ccatctggta agtaggagcg acataccaga
    85621 ctctttgctt tttttccttt gccgccgcac gaataatagt ggagatcgac aaccgggatt
    85681 ttccccagcg tcgaccagca caaacaactt taaatcgatg cggagactgg aagactttca
    85741 tctgtccaga atgcagttgc acaagactga gcgaggacgg gattgccatt attcgtcctc
    85801 cccttcactt ccatcatctg tcgcatcaaa ttcgcttaga gcttcttctt ccaacgtctc
    85861 aagcaattcg tcatcgatga tttcaggctc gtcgtcttcc tgacgtaatt tcgccacctg
    85921 ggaaggcgta agctcaccaa ataccaggtt cggaatttct tcctcgtcat tttccgcatg
    85981 atccatgccc aatgctttgg acgaaacttc aaagcatttt gcaagagtat tactggctct
    86041 ctgtaagctc ttgagagaat cctcaatcgc ccctaaaggc ttaccctcac gtttggccgt
    86101 agtgacttcg accatcacca tctgccccaa cgcatacgcc cagccgtcat aacgtgtacg
    86161 acgttcttct atcttttccg cacgggcttt agcgcgaagc tctgcgtcag atttaagaga
    86221 ctcacgaacc atcttcccaa cagagtccgc gcctttctct aatcctcgct ttttgaaatg
    86281 tctggagagt gtttcacgac gaatgccgta ctcttcctcc agctttgaga gtgtatattc
    86341 gcctgacgtc catttggctt cagcttcggc ccactccgct ggtgtcaggc gagtttttac
    86401 ctcgtctttt tcgaccgtca tagatccctc taaaacacac agagcgcgtc catgcgctct
    86461 aaaacaactt gtttactgca tatgctaacc aacttgtttt ctggggtgtt taattaggtc
    86521 tgggcatgtc ttactaagcc tgcttctgta tatatttaat aagttactta ttatttatat
    86581 atacagaagc aggtctttaa ataagctccc agaccgatta catcaccagt aacttcgctt
    86641 tggctcgacc taaagtggtt aacccaagag tccggcggtg atagcgattg tcactgcgtt
    86701 ggcgcgtatg tcctttctcc accagcccct tttttatcag agcgcgaatt gagaactgga
    86761 tgctttgctt ggttgtcttg tacggcaaaa cttcaagcaa ttcgtccaga tcaagtagat
    86821 gacctcgctc ataaccgaga ttgagcgttt tgatgatgtc cttttgtttg tcggttaacg
    86881 tcatggcaaa tccttatgcc ggtaaagcaa tttctaacgg tttatccaaa ggttgtttgt
    86941 cgaatgccag cagtggcagc gtgtcaggca gtcgacgacc aaagtcaggg tttcggtaca
    87001 caccatacaa cggagacgta aagctcaggt tgtgaatgtc cttgagcagc ttcacaatgc
    87061 tggcctcgtc caccagactg tcggcaatgt cctgaatcgt cgtgccacga ttccgcccag
    87121 cttttgccag ggaactgttc ttgtggtagt ccgccaccag atcacgcagt gcacggcgac
    87181 gacgagactc gctcattgcg aacaactctt tgacgatcgc ctcgttatca ccagggtcgg
    87241 aacggaaatg gcgctggaat acacgcagtg cactttcata gctcttcggt cgctcagggc
    87301 ggatgaactt aaaccctgct ttcatggcga agggattgta tttgctcatc gaggactgga
    87361 tctcaatgat tggccggtca tgcatcctgc taaccaggtt aatcattcga taagagacgc
    87421 ctacgccacg atactgagta tccacaactg agcgactgat caccgcaaag ttgttattca
    87481 cgtaccgacc ccagtactgg ttagctacgg tggtattagt ggttggtttc aacttaggaa
    87541 acatgcggtg gcgaggtgcc aacagtagtt tcgggtaagc cataaccacg acgcccacca
    87601 gacggtcatc aagttcgcag cgatagtaag ttggcgcgaa aggtttgccg tctgttttgt
    87661 agtgaagcga cttaagagcg tgccagtcct ctacagttcc ctttgtgaca gtcattcgct
    87721 ccagaaagtc cagatgacgc ggaaactctt cagggcggta gcgtttgatg atgatatctg
    87781 ccatgtcgat cacctgcgct cgatattggc attgatgaaa tccaggcgaa gagactccat
    87841 cgcccctacc atgacatacg ggcgcccgcc gttatgccag caatcaagaa cactgccgtc
    87901 gttgttgatc atcaacagcg ccaggctctg gctttttcct tctctggcgt gctgaagtgc
    87961 ttcttccagc aggcggatga cttccacgtt gttgttatca gtctctttcg atggcttcag
    88021 ctctacgatc ttcaaatcag gcatattcca ccttcacgcg ttctttgtag tgcttggtga
    88081 tctgcatatc cgggcgcagc gcgttcttca ggtcttcgtg agtcgtcgcc accattaccg
    88141 tcgcaccaac ctttcgagcg gcacgctgga ggttagacgc cacaacctga gcggttacac
    88201 ggtctagaac agcgccgaac tcgtcagcag cccatacttt agcgcctgac tcaatcagtt
    88261 tggcaatctt gagacgatat ttctggccgt ctgacatttc agaaggcttg cgaacaaaca
    88321 gatatgcatc gttcagacca gccatagata acaacccaag cgcatcactg gtcgttttgc
    88381 ccaactgatc gatgacgtta acctcattat cgaaggtaaa atcatcgatg gaggctacag
    88441 acagcccttc atccttcatc tgtcgttgca actcgcgcag cacaacggat ttgccggaac
    88501 cggattggcc ggtgatgtac accacatcgc cctgcttcac ttccagctcc agattgtcgt
    88561 aaagcgtcca ctctttttcg tccaggccaa gcccgaacga ctcagcgatt tccaacgtgc
    88621 gcgtggtttt atttacgcgt gtctgaaacg atacgttgat gatgtatttg ctcatgcagc
    88681 catctccccg gaagagattt tctccgcata tgccacaaat gcgtctaccc cgctttctcc
    88741 cgtcatttct tccatgtggg caagcaaatc accaacaaca atggcagagc cagcaggaag
    88801 cgttttaaag cccaatacgt cgacaacacg tacttcttcc gctgcaactt cacgactgat
    88861 ctcggtgtgt tcatccttct gtcgtttagt ttcttcgcca agatcgataa ctagcgagtc
    88921 ggtgtccatt tcttctgtca tactgccaac gagaacattc aactcacgct cttcgaagcc
    88981 gaaaacctcg atatcgtcca gaacaagcga ctcaagctct ttctgtagct taattgcatc
    89041 gtaatcaatg ctggcaagtc ggttatcttc aaggcgcttc gcacgaacct cgtcatcact
    89101 gagatcatcg cgaacaataa ccggtacacg ctcaagtcca gcaaaaattg cagcctcacg
    89161 acggccgtgg ccagtaataa ttacgtcgtt cttatcgacc gtaattggct ggtcaaatcc
    89221 gcgcttttta atggctgcgg ccagatctct gatctgctgt tcatcatgct ttttggcatt
    89281 catctcatag ggaataagat ctgccgggtc gcgatatacg atttcaaact ttttggtcat
    89341 tacatacgct ccttgtagta gtcgaccagc cacaccagag cctcaccagc gttctccatt
    89401 tcattaccgg tgttaatagc ctgctctttg atgatgtttt ttatggtttc tgcaacacga
    89461 tctgacgcat cgaaagttac tttgaagcgc atggtctgat gttccgcccc cacacgttcg
    89521 gttttctctc gtttgtcggt atcgacaggc tcatcactac cacgagacaa cgcctccagt
    89581 gcttcaaggt cgattgccgc ctcttttgct aaaaccatcg agatttcgtc gtcatacggg
    89641 gcgatttcag acagttgata gtcaagttct gactgaattt cttcaatgaa gcgttgcaat
    89701 gcgatttggt cgtcttcacc gtatcgctcg ttgtccacca gtgacatctg tttagctacg
    89761 acatcgctaa ttttgcccac agaaagcact ggaaccgttg aaattccttg ctcaatagcg
    89821 gcacgccagc gatgttcgcc gccgaggatt tcaaaaaatc catcttcaag ttcacgagcc
    89881 aaaattggct taaaaaagcc caatttttcg atagaacctt tcagtttttc gaaattctgc
    89941 gcaccaaccg aattggtgtt ccaggtattc gggcgaaggt tggcaacatc aacctgcaaa
    90001 atcgtgattt ttacgtccat tttattgcta caatccacta agtaatcact tacttattat
    90061 aatagccaaa taacatacaa aaggcactaa ggaaagaggt ttatgactgt tcggattgta
    90121 tctaacgcag taaatgcgct tatttctggc gcagatgaca aggtaaagca actggtgcag
    90181 caaatgttga gctacgaagt cgaggctggc gactggaagg gcacaagcac aatgttcaac
    90241 tggagcaaaa actcgttccc tgctggcttt gccaagcccg tggcggctaa tttgaacaag
    90301 gcgggcatca aatgtgttca tatccgcaaa gacaaagccc cggcgcttgg taaaccaaat
    90361 ccggcggtta acccattccc atacaatcct gattatgcgt atcaggatca gactgtggaa
    90421 acactggttc gagagggaat gatgattgcg cagatcgcta ctggtggcgg gaaatctaac
    90481 gttgcctgca aagcagctgc acgtatcggt cgaatgacat tatttttaac aacccgctct
    90541 gttctgatgt ttcaaatggc cgaaaacttc cagagatcca tcgactaccg cgccaaaaat
    90601 ggcgaaccgt ggttaaaaga ccaaaaggtt ggagtcattg gctcgggtga gttccaggta
    90661 tcacgacata tcaacgtcgc tacagttcaa actcttgcaa gtttcctcga agaaccacca
    90721 cgcgatgcaa caccagataa gaaaagctac cacctcaaac gtcgggagtt ggtgaaacgc
    90781 ttcctttcaa gtgtctctct tcttattctg gaagaggcgc atgagtcttc aggctcaaat
    90841 ttctatgaca tcgccagatt atgtgtgaac gcagactatc gtctggcgct tacagccacg
    90901 ccgttcatga aggattcgac ggaagccaac atgcgcctga tggcggtggc cgggcgaatt
    90961 gaaattaaag tcacagaaaa gtatctgatt gatcgaggca ttctggcaaa gccgtacttc
    91021 ctttatcata aagttgccta caagccagac gaggccagaa tcaaggccga acttgccaac
    91081 aaacacctca attttagagt tggtatgagc accgcctacc aaaaggctta tcagttgggg
    91141 atcgtgtata atttgggacg taacgaggcc attgtgcgcg aagcattgct ctataagcaa
    91201 cattctctca attgtatgac tctggttcgc cttaaacgcc acgggcaaat cctgatggaa
    91261 atgatgaaag agtccggcct tagagttgac ttcatctatg gggaatctaa ccaggcgaca
    91321 aggcaagcaa agctgaacag tttagcgtct ggagaaatag atgttttaat aggctcgact
    91381 attctggatg tcggtgttga tgtgccaagc gttggtgcgg tcattcttgc tggtggtggg
    91441 aaagcagaag ttgaaatgag gcagcgtgtc ggtcgtggct tacgagccaa gaagaatcag
    91501 gcaaacgtgt gttttatcac tgatttcatt gacattagca acaaatacct gttgtctcac
    91561 tcttatgagc gaaaacacat catcgacacc acacctggct ttgcagaagg tgtattgcct
    91621 attgatggcg cattcgattt tggagttctg aaacgagatt agttatgagc gaaaagaaaa
    91681 caacttattg tcaggtggca ttgtccgata aggccaatga caaacttgga aagtttcaaa
    91741 tgaaactaaa agaaaaaaac atcaaaatgt ctaaggctga agttatcaat accattctgg
    91801 agacgatgac gatggctgaa tttgacaaag tttcatctgc cgttggagtc tctgctaagg
    91861 ctcgtgagaa aatcatgcgc atctatgaga actctaatat gacaaaggaa gatttagagc
    91921 aaattctaag caaactgccg taatattgga ttcttagtca atttcagaat tgccagactt
    91981 gaagagctaa tagacagagt gcggcaacaa cgcctttgcc gcacagcttt tagagaaaac
    92041 acgtctgcta tttacagttg aaaacttatc gtttgagact attggatatt tataattcta
    92101 ctttgcaata atcgctgtat aaaaatgaaa cgttcgttac ttcttgcttg tatatacctc
    92161 tcgtcatttt ccgccaatgc gttgtcagat atcgtctccg acatgcaaaa aacgtacagc
    92221 atgtatactt ctgaagacgt actaaaatac gaatcacgag gtctgattca accagtctgg
    92281 ttcagattct cagataacgc agaagaaaca atacttaatg caggaaaaga aagattacgg
    92341 ttgcaaatta acggaaccgc tgtaccagaa aacgttaatg taagctctga cgaagccatg
    92401 aagccatctg gaattgtttt ggaagttcaa gacggcacac tttactgtga tgaaatggct
    92461 acaacaggtt gccaagtaaa cattagggtt ggcaatgaag agcctatcgt tacttacgcc
    92521 aaggctgaag ggaatacaag tggaaaggta ttgtttatcg atgaaaatac ctcaaaacgt
    92581 atcgttcgtg acatacagaa aacagcgaac tctaaatccg tcgataaaaa aatctatgtt
    92641 gaggttccct tctttgtcgt tggtaacaaa gaatttaaat ttgggttgct gaaaacacct
    92701 ctaagagtat cagaatgaga acattggaca tcacctacgg cataccggct gaagtatggc
    92761 cgcgagacta cgaacatgct gaacgtacct tgctcttctg gcggagtgca aaatttcctg
    92821 taagagtcac tctcgaagac ggccaagtgt tttgcatgta tgtctacggg ctattgagcg
    92881 caagaaataa gctgaacctc acaccagatc cgcttagcaa cacaaatcgt ataaggctcc
    92941 ctcttgagcg cattagcaca attgagtcgg gcatagaaga ggttgacact tcattcacag
    93001 gtcgactaac gctaaacaat gaacagatgg ttaatcagcc atcacgtcgt gactttttta
    93061 agatatgtcg tcaggcttac caaaacggca aatctataag ggtctacatg gcagacggtc
    93121 gcgaaattga gggaacgtca gatggcgttg acgcctgtct agtcacactt aagttggaag
    93181 acggtagaaa aatacttatt ttcttcgatt gggttgaacg aatccttccg ttttaatttc
    93241 tataagggta ataaccggct aagtccggtt attattttac atgccattca ttttatactc
    93301 tttcaattct accccgtata aatatataag gtctaaagcc ggaatcgatt atttatttag
    93361 ggaacaccct cgacgatcta acatttattt ctaggacttt catccctgca aaaaaactta
    93421 aaaaaacact tgcaatcttt tttcgcgtat cgataattga acgcatcagg aacgagtgca
    93481 aaacactttc tttcttgagt taaattttaa tatttatact aaggaataaa atcatggcta
    93541 acattatcat ttctaaaaag tccatcattg aagctgcatc tattgtatcc gacgaactgc
    93601 gcgaaaaagc agatctggca actcaaacat ataacgaaca ttataaaaat ggtacgcaca
    93661 ctaaagcaga caaagcaaat atgcaagctg cgaccactaa acttgcttac ttcatcaaca
    93721 acgtcgtaaa cgctgtagaa gacgaaaaat tatgctctgt tttctactat gcgattaaag
    93781 caagcaaaca agcgccagaa gtattttttc gtgacgctat gactaatagt tattcactgg
    93841 aaaaactggt ttatctggtt aaatcaatta aagctggaaa atgtgtttat tccgtcgctg
    93901 atatgtctgg atctcgtgta ttcgctttaa tcgatatgat taacgacgag atcgacacgt
    93961 tcacgaatgg cgctgttttc gatttaatga atgaagccaa aaaagcgtgt gaaattaaat
    94021 tggacgctgg ctatactcaa gctaaccagc taatcaatct ttgcgaacgt ctcggacttg
    94081 ttgagaaagt caaaggaatg ggaagcgcca aagctggtac acagcaatat cgctttatca
    94141 agaatgattt ctacaattat ctagctgacg ctttcaaagc gtaattaatg gaatcaagcg
    94201 cccactatgg gcgctttaaa ggagctttaa aaaatggtca gctatgacaa gatccgcgcg
    94261 gaatatcgcg caaagtatcg cgcttataaa ctagaattaa tcaatgattt aatcgcgcaa
    94321 cgcgaccaat taaattttac gttttctgat ttgcttaaca gcaaacgaga ttgcaagcgt
    94381 aaacgtgaat acttacgttt aagcgcatta atcggaaaac ttcataattc tatttaattt
    94441 ttttaaggag cttaatcatg gttattttca tctctggcgt aaatatcaat aatcatactc
    94501 ttgtttatga tattgctggt ttagctggat atgctctaag ttctgaagtg gttgatgaaa
    94561 caacgtttaa aataaacatc aatgacgtag aacatagagc gcgcgcagga atcaatgaag
    94621 cagatgttac tttaatgctt caagaatttt taaacgctgg ttttaatatt cgcttagaaa
    94681 aataaaaaca atagcgccca tagtgggcgc ttttttcgtt tccaatactc ccaccataac
    94741 gcgccaatga tggcgcgttt tttattgtct ttgactcact ccaacaacat aaaaataagc
    94801 gccaaaataa cgccatagac gcgcttttat atccttgcca gtacataccc attacttaac
    94861 acattaacgc gcttaaaacg cgttatattg cgctatagcg catagttaat cattggcttt
    94921 tagtcttgct atgtgatccg cgtttatttg tcggcgcgga tcggcatttt gttttgttcc
    94981 gtatccgctc gcgtattatg tgcgcgtgat ttttcacata atcacaccac ttaatcacgt
    95041 atgcgattac gctacgaaag aaatctggat gtctcaggcg acgaaagtca tcataatttt
    95101 ccccgctcac ctgtccgaca accgctggtt ggattccacc agcttcccga tgttttttct
    95161 acataaaggc gaatgcagcc gtttcccgaa aaaatcctgg ccgttccccg tcggttcatg
    95221 aatacgttcc tcgccgtttc tgaaaatttc cctgcggcat ctggtggcta tagagaaagg
    95281 gccgttactg gccctcttct cagttacacg ccatcaagga tgtggatgcg gttgcttgcg
    95341 tatacattca gcataaagtt agcgcaaaac agtttccatg tatcaacgcc agcggtatac
    95401 gtaatgtttt tgcatttaat tgcattgttg gcgatccgca tcccctgcga tactgcttca
    95461 tcgtcggaaa atttgaacga cgattgagtt ttaatccaga cagaaatctg cgtagcgaac
    95521 tcaatcagct tggactggca gaatcgcccg gagcgcaccg gaaagacgaa cgttccgaat
    95581 ccagaattta ccacatacgc tttctcaaat acccgcgagt aacgacgatt gccaatgatg
    95641 tcgcgtgcaa taatgcattt ttcttgtgct gacagttcta ccgtctcatt gtcgcgccat
    95701 gcaccaagta ctcgtttttc aatgtcagag aacgttacag cgatattgcc atgtgcgggt
    95761 gcgtttacag tagcgataaa attcatgatt aattccttat cgtaaataac aaattgtttt
    95821 cttgttggcg ttaattatcg ttgtacgaat aaggcgtcaa agtggaaagt tgcggtagcc
    95881 ggacgggaac aggtgggttt gtcggttgcc tggaggtaag aggttggtgt ttttagcctg
    95941 cgggaaacag gatggtcatt taaggccacc agcagtggtg gccttaataa ttatcgaccg
    96001 attacgctaa ggatcttttc ctcaactgat ttgttgttgc gactaaactg ccttgcgtat
    96061 ctgatgacag aagaggcgtt ttgctttctg actatctcgg ctctttcctt caggcgtttc
    96121 ttgaaatcgc ccatatttac caccaggcgc agaaggtcag tctgttattg tgaaagtcgt
    96181 ggttcttaat cagctcttcc acaagttttt tcaactcccc cacgtcatgc cagtaccctt
    96241 catcgtactc ctgactgccg aagaaaaaac cttcacaggt aggtaagtac tcttcgcagt
    96301 tactttcgtt catgtgcatc aaatgagctt tcagagcaca aatgtcattc atcgttaatt
    96361 ctaaaagctc acaatttttg acttcaccta cgttgcgttc catccaccca aagagagcat
    96421 tgaacttacg gaagtaagca accttctttc tggatgcatc gttattcaga tcgttttttg
    96481 gttgcgtctc aatgtagata tcaagtccca tgatggtttc cttatcaagt tgctttcagt
    96541 gaaagcattt tcacaaatca gataaggcag aaaacaaatt gttatcgggc ataagaaaat
    96601 ggcgcggttt acgcgccatt caaagaggat taagcgaata cgctttcagg gattaaggtg
    96661 tcagcaggaa tgccggaggt aatgcgcaga ccatactgac caatccaggt agtgttagtg
    96721 ttcagactgg aagcgaatac ggtgttaacc atattcatca tggtttcaaa cacctcttta
    96781 ttcaccttac ggaagtaact ttcaattttc aacaacaacg ggtccgttgc atcaaaaatg
    96841 gactgatatt gcgcctcata ttccccgcct gatgtatctc ctttacgcac aatagtactt
    96901 gcctgaactt cagttccagt ttcggtgttg tacgtaacga cggtgatttt agctacattc
    96961 ttaccttcgg cgctttctga agcatagtag acatcaactg tcaggttttc tcgtttaacg
    97021 ctcatatcac tctccatagt gtcttggtta tttttctatt ttatagacat aggtatgtac
    97081 ttacaataca aaaaaccccg taaggatggc ggggctgtcg tactgtcgac taattaccac
    97141 ttgcacatta tcaggctatt gccatcactg atgctctctg aatctcttgc tgggccacgc
    97201 ggttaacttc catcaaagcc agttcgagct gatgttccgg ccacattctt tgaaatgcca
    97261 tccagccttt tccacgctgg cgacgaacat tcattacata acgagtaaag gtgtttttat
    97321 cgaaagacac cgctgtttca cggaacagac gaatagaggt tccattggca acgatgtcca
    97381 gcaaagttaa ttgagcaacc agtgcaggct tctcttttct cttctggtct ttgtccagac
    97441 caagaaccaa tgattttttc atcacaactc cgtaaacaac ttgttttctc aatggcgtaa
    97501 ataataccag taagaaaacg gctacaaagc atattgatca ggcgcatgag cgatatgcag
    97561 gttcttccct gcggctacag atgaagttaa gccagcaatg ctggctttgg ttgggcttac
    97621 gcgaatatcg caaggaagtc ctgaacgtcc caatgctgga cgaggtgaag atagacctta
    97681 gaaatcacgt cattttcgct atcgcatgtg aagtagtact tctggctgcg gttgtatgtg
    97741 tccacacaaa attcgctcat atccataaag tcagcatctt cacccatgtc gcaaatcatc
    97801 aaagcaggtg cgtcatcacg agtcatcagc tccagcaccc atcttgaatt aatcagagtg
    97861 gaagaccaac cttccagctc ataaaactgg tcaaactctt tttgagtcaa tttcttaatt
    97921 tggttgatgt cgatatttgc aaacataaag caatccttaa acatgttgtt ttctgaatta
    97981 cgtaaacaat accagtaaga aaacggccac aaagtatatc gttacggtgc tgcgctgccc
    98041 cgaggttaat agcgacaaaa gccaccgtgc tggtggcctc actcttaatc ttggtcgtcc
    98101 gggaactcgc tgtgtaccgc gcccaccagc tcccgctttt catcttcact cagcaggtgc
    98161 cagatatcct tccctttggg cgattcccct tctgccggga cgaacgacca caacttgcgg
    98221 tacaactccg gccccactgc atccagacac tcagccaggg aatccacgcc ccacacctca
    98281 accgtcacag gcatcttaat catctcttca tcctcacttc gtttctataa cgtctaaata
    98341 tgctgtttac ttgttggtgt tattatcgca ataagataaa ggcgaaaaag gattttatgt
    98401 cgggaaagcc aggctgggca gtgaatatcc gggaacgaca cgagagccag tatttacgat
    98461 attctgctct acgcgcgccg cggggctggt gggtagaata atcctctccc cgaaaaccta
    98521 ctgaaatcga ttgtgacccg ctggctgtct ggcacaatta cgctgtatct ggcattcaga
    98581 gggaatccag cggtaagcaa gtcagcctgc acgtgagcgc ctctcttctt cccgcacatt
    98641 acaggtgggt agctgcaact ctctctctct cgctataccg gtacgccgcc gttccagaac
    98701 cggcatcgag ccttttcccg aaatccacac aggcaactcg accgtttccc tgaaaatccc
    98761 cagccgttcc cccgaaggca acccagccgt ttctgtagct ggatgcaacc ttttccctgt
    98821 atggaccgaa ggctgaccgt agggggtgtt acgggggata aaatcaggag ttgaataaat
    98881 acgaaaaagg aggcgacccc cctccttccc tctccactta tacatacctg gcttcttcgc
    98941 tatacatgtt gtgttttatc tgtctcaacc tctctatagg cgttaatgct cactccttcc
    99001 agaatgttct atacggcgac tatttctgtt ggtgaattgg gtgtgttgtt ttctctgtcg
    99061 gtgtatgtgg tttcgttagt tttgcggtgg ttcttctatg ggggtgttct cgtcgttttg
    99121 gtgattgttc ttctccgtgt atggagtaat ggcaggtggg cgattgcttt cgttattgtg
    99181 tttgagttgt ttgtctggag tgtgtgaagg cttttgtagt tattcgtgta ttgggtaatg
    99241 gcgtttcccg tgattgttat gtttcgtgtc ttttgggatt gagagaaacc ttgcggttac
    99301 aggcttcgtt agggtttcag cggttcaggt gtttcttcgc tgtttgggta atggcgtgtc
    99361 gtgggtatcg cgcgtatgaa tgttttcgga ttgcctgtga aagttaggtt ggttgtccgg
    99421 tagcctgggg agaagaggtg ggtcttttcg gtagcctgcc ataaagaggt tggttgtttt
    99481 gggtaatggc atatgtgtta tttggttgtt ttgttatgcc tgaaaataag ttgtttatat
    99541 ggttgtgtaa cgcaacggga gcgattttga gcgtgtgttt ttgttggtag tgtgttgagt
    99601 cgtctgtggt gaaatttgga cgctgtggcg ctatctgctg cgtagaatta cgggtataag
    99661 aagtgatgtg tgataacgtc aattttttag accaaatcgg gagaaagcgt tgatgtttcg
    99721 ttgatctgtt atttagttgt tttcttattg gtataaacaa ccataaccta ttgatattta
    99781 atgatgtgat tgctggtatg ggagggtagt agtggcgatc aacgggttct tatagaaaag
    99841 aatcaaattg tgaccgccat tatcaacggt ttgattagat gagatggtct aattcatcat
    99901 ctatcatggc tttgacgctg gagtcgttca ctccatattt atttatgagc tgtttcagct
    99961 ctgtcgaaaa ctgaaggtag atatcgtgag cgtttttcac tgtaggttca acgccactca
   100021 tttcaagcgc ctttagtaga tatgagtctt ctttaagcat aaattctccg ttatctagta
   100081 tgaagcgatt gccggattac cattgccagc actcaagccc ttcaacgacc ggtgcgccac
   100141 aatcaaaatg tacagagtta aagcctacat caagtacttt ctgaatgttc gcaatcgcac
   100201 cctcggatat accatatcga cgcaactcat ctttccagtc atcgtcctgt aacccggcgt
   100261 ggacaatgca accgtactca atttgtgaaa cccaatccag ctcattgctt gcgatcatga
   100321 atcggcgagc atcttcctct gatgtatgag atgaactgat caatgccacc tggtaggctt
   100381 gggtaatgtt caacatttca actactccaa aacaagttgt tttcttatgt ggcttattat
   100441 ctcaatcaac aaaaggtaga aaacaaattg ttttagggct tgtgtaaatc aggagggatt
   100501 gtgtctatat gaaaatgccc ggcgtgttgc cgggcattgc gaagagaggt tgtttaatta
   100561 cttaacctgg tggccaggtt tagcaccagc atccgggctt aacaggaaga tatctttccc
   100621 gccaggaccg gcagccatca ccatgccttc agagataccg aagcgcattt tacgtggtgc
   100681 caggttagcc accataatgg tgtgacgacc aatcagtgcc tgtggatctg gataagcgga
   100741 acggatgcct gagaagacat tacgcttttc accaccgaga tccagcgtca ggcgaagcag
   100801 tttgtcagaa ccttcgacaa actccgcgtt ttcaatcagc gccacacgca ggtcaacttt
   100861 ggcgaagtcg tcaaaggtga tggtttcctg aatcggttcg tcagccagtg ggccagttac
   100921 cggtgcagct gtggctttca cttcttcttt ggatgcttcc accagtgctt ccacctgttt
   100981 catatcgata cggttataca gtgccttgaa cggattcact ttatggccca gaagcggttg
   101041 ctggatacca tcccaggtca gttctgtatt caggaatgct tctgcacgtt cagtcagttt
   101101 cggcagtacc ggtttcaggt acgtcatcag cacgcggaac aggttaatgc ccatagagca
   101161 aatcgcctgc agatctgcat cgcgaccttc ctgcttcgcc accacccacg gagcctgttc
   101221 atcgacatag cgattagcca ggtcagccaa tgccatgatt tcacggatag ctttaccaaa
   101281 ttcgcggctt tcccatgctt cgccaatcac ttcagcggca tcagtaaagg ttttgtacag
   101341 ttgcgggtca gccagttcgc ttgtcagcac gccgtcaaaa cgcttgttga taaagcctgc
   101401 attacgggag gccaggttaa ccactttgtt cacgatatcg gcattcacgc gctgaacgaa
   101461 atcttccagg ttgagatcga tatcatcaat gcgcgaagag agtttcgcag tgtagtagta
   101521 acgcaggctg tcagcgtcaa aatgattcag ccaggtgctg gctttaataa aggtgccgcg
   101581 agacttggac atcttcgcgc cgttcaccgt cacatagccg tgaacaaaca ggttggtcgg
   101641 cttgcggaag ttgctgcctt ccagcatggc aggccagaac agactgtgga agtaaacgat
   101701 atctttgccg atgaagtggt acagctcggc ggtggagtct ttcttccagt attcgtcgaa
   101761 gcttacgctg tcgccgcgct tgtcgcacag attcttgaaa gaacccatgt agccaatcgg
   101821 cgcgtccagc cagacgtaga aatatttgcc cggcgcgttc ggaatttcaa aaccgaagta
   101881 aggggcgtcg cgggagatat cccactgttg caggccagat tcaaaccact cctgcatttt
   101941 atttgccacc tgctcctgca acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa
   102001 agagggcaga tcaaagaaga agtgttcaga atcacgcatt accggcgtag cgccagaaac
   102061 caccgatttc ggctcgatca gttcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt
   102121 atcgccgtat tgatccgggg atttacattt cgggcaggtg ccttttacaa aacggtccgg
   102181 caggaacatg catttttccg gatcgtacag ctgtgagatg gtgcggtttt taataaaacc
   102241 gttttctttc aggcgagagt agataagctc ggacaactga cggttctctt cgctgtgcgt
   102301 cgagtgatag ttgtcatagc tgatgttaaa gcctgcgaaa tcagtctgat gttcctgact
   102361 catttcgcca atcatctgtt ccggtgtgat accaagctgc tgtgctttca gcatgattgg
   102421 cgtaccgtgg gcatcgtcgg cacagatgaa attaacctcg tggccgcgca ttcgctggta
   102481 acgaacccag acatcagcct ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt
   102541 agcgtacggc agcgcgcacg tcaccagaat tttcttcgcg acttgggtca tagtaggcat
   102601 tacttctttg tagtgaaaag gggcttgata gtaacaaaat ggccttatgt ctgccatgtg
   102661 ataacagcat ttctcataaa tgattaaatg tcgtagctgg agtacactac aaggcgacaa
   102721 ttgcgcaaat taaaataaag gagacgggat gagcgagtcc aaatcgccgg atgccctgag
   102781 agcaatggta gccggtacgc tggctaattt tcagcaccca accctgaaac acaacttgac
   102841 tacgcttaaa gcgttacacc atgttgcgtt gatggatgac acactgcatg tcgaactaat
   102901 catgcctttc gtgtggaata agccttttga agacctgaaa gagcaatgta gtggtgatct
   102961 gctccgtatc actggcgcaa aggctattga ctggaagctg tcgtacaaca ttgccacgct
   103021 taagcgcgtc aaaaaccaac caggcattaa tggcgttaag aacattatcg ccgtcagctc
   103081 aggcaagggc ggcgtgggta aatcctccac agcagtaaac ttggcactgg ctttggctgc
   103141 tgaaggtgcg aaagttggta ttttggatgc cgatatctat ggtccatcaa ttccaaccat
   103201 gctgggcgcg gaaaaccaac gtccaacctc accggacggt actcacatgg cacctatcat
   103261 gtctcatggc ctggcaacca actctattgg ttatctggtc accgatgaca atgcaatggt
   103321 gtggcgtgga ccgatggcga gcaaggcgct gatgcagatg ttgcaggaaa cactgtggcc
   103381 tgatctggac tatctcgttc tcgatatgcc accgggcact ggtgatattc aattgacgct
   103441 ggcgcagaac attcctgtaa ctggtgcggt tgtggtaact acgccgcaag acatcgcgct
   103501 gatcgatgcg aagaaaggca ttgtgatgtt cgaaaaagtc gaagtgccgg tactgggtat
   103561 cgttgaaaac atgagtgtgc atatttgcag taactgcggt catcacgagc caatttttgg
   103621 caccggtgga gcacagaaac tggccgagaa atacaacacg cagttgttag gccaaatgcc
   103681 acttcatatc tctcttcgtg aagatctgga taacggaaca ccaaccgtta ttagtcgtcc
   103741 agatagcgaa tttacggcta tttatcgtga cctcgcagat cgcgttgctg cccaaatgta
   103801 ttggcaaggt gaagtaatac ctggcgaaat cgcattccgc gcagtgtgat aaaaaggcgg
   103861 tgcattgcac cgcctaatca gttctcctac atcacaggac aatcatcaaa ctcgccactt
   103921 cttgcgtcgt tgataatgtg agtgatcaca ccaaacacgg cattgctgcc cgtgcatcca
   103981 tcgtcatcct ttggcaatgc ttccttctta ccggttctta aatcctccag atgctgacgt
   104041 ggagacttcc ggtatctttt tacgcgatat tccccatcca gagcgcatac aagcagagaa
   104101 ccatcaaccg gggtaagtga ggaatcaacc accagcaatg caccctgtaa tattccctca
   104161 cgatgatgac tatcagctgc tctcatgaag taggtcgctg aaggatgtct aattatctgc
   104221 tgatcaagag aaatacggct ctcagcataa tccgccgcag gagaagggaa gcccatagcg
   104281 ttttgccctc aatactgttc atttacacag tatacatgaa agaagctcac tcagtgcaac
   104341 tgatcggtac atagcaccag acaaaggaac gttattttct cgaacaagaa aaggtagaaa
   104401 acaaattgtt taaggccaca actagtggcc ttaatggaga agggatgctt attggctatg
   104461 gctaaaaatc aaatgagaca cttcatttgg ttgtagctcg tcaagatccg gggccacaaa
   104521 accttttccg aaagacttca caagtgattc accagccgca tcattgtcac ctattacgcg
   104581 aaagtcatac ggcagcattg ccagttgacg acgtaatcct gctgagaaag tcgaacctaa
   104641 cagcgcccac gaatttccgc ccgcgttgtg cactgctgca cttttaaaaa tcgactcagt
   104701 tatccatatc ggtccatctt ttaaaggaag ataggttccc catagtagct gtctgccgtg
   104761 cgctcgtgtg aaataccggg ccatcttcgg attgctatgc tgcttaggag cgcctggtct
   104821 gtatgtctga tacccggcca acatcccatc aaacctgtat agagggaatg taacaactcc
   104881 ttcgctttca tcgatccact gtctatatgg atgcacatca tagcggtagt tacgagttag
   104941 caaatgagct tccagctttc caaatttagt cataacttcg catccctcat aacacttccc
   105001 atttatcacc atatttctcc atagctctta gccaataaac atggccacta ggcatagtga
   105061 tgtgaggctc gccaaactct tctccatgta catcaaagat gtccccaaca gaaaattggg
   105121 ggatttctgt tccgttttca cctatatgtg taattcgtat acgtttcggt tgggattgtc
   105181 cacctttgag cgagttgttc actgctctgg caattttctc ccgcaattgc tgcgttccgt
   105241 gatacgtaac cgcaatatcc cgcaactcat tcactagttc tcgaatttgg tgatctttaa
   105301 gctcattatt tgggtgattg gtaacaactc tggttaattc cccattgtct tttgctctta
   105361 cgtatgcagc tcttgtgcat tcgccccatg tattaccgcc tacattaact tcgtagatta
   105421 ggctggactt atctgtcaat tcggaacaaa tacgggccgt ggtctggcaa ctagagcatt
   105481 cgcagttatc gcggaaaccg tggtctactg gcttcggttt aacatcatca actttttcta
   105541 ccctattgga atttaaatac ttcgaagcct tgctatgcca atcgctggtt ttaggatctt
   105601 gtccatccat caaacgccag gcttcagcta ttaactcata actagtgact gtagcttcct
   105661 gcgcatcacc aataagtcgt gtaatctctc tttcaagccg accgcctaac tccgttttag
   105721 tgcaatgttc tgaccaatcg ccagtctcaa gaagcgcgag aatgcttaaa atatcgtcca
   105781 atagaacggt agtattatcg acttccttct gaaactcacc ggctactgat ttatgcatca
   105841 ttcgtcatcc tcatccgagt catcctcatc acaggatgag agtagtgggt tagtcattcg
   105901 ccctacttga gtggcgtagc aacgccgaca gagattgcgc agcacactgt atatttcgaa
   105961 catctcggtt cgttcatcgc caacatcaag ctcagatgct atagcgtggc attcagtcgc
   106021 gagagccgat attttctgaa ataactctgc tttattcact cttcgactcc tgcggaggtt
   106081 ctggtagcgg catccagtga gttgcttgct caataccatt acccggctta atcgttacat
   106141 ctccgcgccg aaaggtgatt ccggtatagc gtgcggagca tattagcggt tcaaccagag
   106201 agctatcgaa attcaccgaa ataagcacgt tctggccctt ttcaggcatt cgctcactac
   106261 agcttatcca accatccgga gttaccggat ggggtccagc gaactcggga atgtcaggac
   106321 cttttctgat agctttagcc agatccagcg ggtcatcgta aagccagtcg ccagtttgtg
   106381 ggtgatttgc ttctgcaagc tgcgcagccc attcaagacc atctttttga ccttggagat
   106441 aatcaagcgg caactcttca tgattacttg caggttctgc actatcagct tcgcgccgct
   106501 tctgtagctc tgctgccatc gctctcacga cttcaactgg tgcccttgca gcaaactcta
   106561 tgttggtgat cagctcatta agatattgct cgcctggata ctgtttctta tcggttatag
   106621 tggtcatatc actctccttt tccctgaagc atagcagcgc ggcaggcatt ccagcctctc
   106681 acctctgcaa tagcggcaac agcatcaacc gcgtacatgc taagaggatt aggcattggt
   106741 ttttcttccg gtactactgg aacgggtgga gcggcgtaga cctcaataat tccattatca
   106801 ataggccatt ctccatcctt gaggtagtca cttgtgccgt caacttgctg ttctgcaatg
   106861 tggaatgcac ctattggttt tgcttccagc gatgccagag caattcgtgc cagttcttcc
   106921 gcttcttctg ctggcagtac aacgttgcta ccaggtccgt atgtttcgcg ccactgtttg
   106981 attgtcagta gtcgctcttt ggtaatagtg gtcatgtgtt actccttaac ccgcagtgct
   107041 ttcaactgat gaggggaaca aaatcctttc atcaaatccg gcattcatat catggacagc
   107101 aacacaccaa tccatcgacg aacgattatc aagagcctcc atgatttcat ccatgcggcg
   107161 caggtcatac aggtaaatgc ttttatcgcc aatggtgtaa aaaccaattt ttttcggtga
   107221 tgggcagcga tcaagaacgt cctgtaattc gttcaaccat gcccgttctt tttttgttaa
   107281 agttgccata tcactctcct ttgatgcgaa tgcctgctgc aatgctgttt atgatgctgt
   107341 cagtgcatgg agtagaaagc tgggcatctc cagcaatttt catgacctca acatccgcat
   107401 atcgaatacc gaggtgtatc agaccggcta tacctgactt aagtcgaaca ttttccataa
   107461 acagatcttt ttcccgctgt ttagcggctt ccaactccat cgacaatttt tccaactgct
   107521 ctttatgctt cttgtattcc tgatacgcgt gccaagactg acctttgcgc acactatcag
   107581 tgatatcagc aacctgctcc ggtgttagcg tggtcagtgg ctgtgctgga aaaatcatca
   107641 ctttcccggc atcccaatca aaacccgcct gaattgactg aacctcaact gatggtgttg
   107701 aaccgatgcg accaggcgaa tgaacaacga tcgttacatc catatcgcga cgatggctgt
   107761 ggttgttgga caaaatacga ttcaccaact cagaaaattt ggaaaatttc atgctgatcc
   107821 ctctttctct ccccggataa ttctgttgca cacatccaca cactcgtcgc agatgaaaac
   107881 ggcgtcccca gcaattaact ttttgacggc gtactgggat ttgttgcaga agctgcaaca
   107941 aagtgttcgc ctgactgata aggatggagc gccagatgtc agccgctcca tatcgttctt
   108001 gcgacgcaat atcacacggc tgaactcaac cagtttctcc ggggatatct cctcctccgt
   108061 agcaagcgcc tctaatcgtt cgagtatgtg gaaggctttt t
//
LOCUS       DAZPJR010000002        80158 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPJR010000002 DAZPJR010000000
VERSION     DAZPJR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 80158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.003"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            66..350
                     /gene="pduA"
                     /locus_tag="ACHVB7_000127"
     CDS             66..350
                     /gene="pduA"
                     /locus_tag="ACHVB7_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR1912240.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            347..1159
                     /gene="pduB"
                     /locus_tag="ACHVB7_000128"
     CDS             347..1159
                     /gene="pduB"
                     /locus_tag="ACHVB7_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFR1912241.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            1178..2842
                     /gene="pduC"
                     /locus_tag="ACHVB7_000129"
     CDS             1178..2842
                     /gene="pduC"
                     /locus_tag="ACHVB7_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR1912242.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2852..3520
                     /locus_tag="ACHVB7_000130"
     CDS             2852..3520
                     /locus_tag="ACHVB7_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR1912243.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3535..4053
                     /gene="pduE"
                     /locus_tag="ACHVB7_000131"
     CDS             3535..4053
                     /gene="pduE"
                     /locus_tag="ACHVB7_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR1912244.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4063..5895
                     /locus_tag="ACHVB7_000132"
     CDS             4063..5895
                     /locus_tag="ACHVB7_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR1912245.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5885..6235
                     /locus_tag="ACHVB7_000133"
     CDS             5885..6235
                     /locus_tag="ACHVB7_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR1912246.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            6255..6530
                     /gene="pduJ"
                     /locus_tag="ACHVB7_000134"
     CDS             6255..6530
                     /gene="pduJ"
                     /locus_tag="ACHVB7_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR1912247.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6554..6976
                     /locus_tag="ACHVB7_000135"
     CDS             6554..6976
                     /locus_tag="ACHVB7_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR1912248.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6979..7611
                     /locus_tag="ACHVB7_000136"
     CDS             6979..7611
                     /locus_tag="ACHVB7_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR1912249.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7608..8099
                     /gene="pduM"
                     /locus_tag="ACHVB7_000137"
     CDS             7608..8099
                     /gene="pduM"
                     /locus_tag="ACHVB7_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR1912250.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8103..8378
                     /gene="pduN"
                     /locus_tag="ACHVB7_000138"
     CDS             8103..8378
                     /gene="pduN"
                     /locus_tag="ACHVB7_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR1912251.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8390..9397
                     /gene="pduO"
                     /locus_tag="ACHVB7_000139"
     CDS             8390..9397
                     /gene="pduO"
                     /locus_tag="ACHVB7_000139"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR1912252.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9394..10779
                     /gene="pduP"
                     /locus_tag="ACHVB7_000140"
                     /pseudo
     CDS             9394..10779
                     /gene="pduP"
                     /locus_tag="ACHVB7_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            10790..11902
                     /locus_tag="ACHVB7_000141"
     CDS             10790..11902
                     /locus_tag="ACHVB7_000141"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR1912253.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            11899..13244
                     /locus_tag="ACHVB7_000142"
                     /pseudo
     CDS             11899..13244
                     /locus_tag="ACHVB7_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            13247..13801
                     /gene="pduT"
                     /locus_tag="ACHVB7_000143"
     CDS             13247..13801
                     /gene="pduT"
                     /locus_tag="ACHVB7_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR1912254.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13801..14151
                     /gene="pduU"
                     /locus_tag="ACHVB7_000144"
     CDS             13801..14151
                     /gene="pduU"
                     /locus_tag="ACHVB7_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR1912255.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14156..14599
                     /locus_tag="ACHVB7_000145"
     CDS             14156..14599
                     /locus_tag="ACHVB7_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR1912256.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(14700..15029)
                     /locus_tag="ACHVB7_000146"
     CDS             complement(14700..15029)
                     /locus_tag="ACHVB7_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR1912257.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15201..16259)
                     /locus_tag="ACHVB7_000147"
     CDS             complement(15201..16259)
                     /locus_tag="ACHVB7_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR1912258.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16457..16930)
                     /gene="sbmC"
                     /locus_tag="ACHVB7_000148"
     CDS             complement(16457..16930)
                     /gene="sbmC"
                     /locus_tag="ACHVB7_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR1912259.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17049..18215)
                     /gene="dacD"
                     /locus_tag="ACHVB7_000149"
     CDS             complement(17049..18215)
                     /gene="dacD"
                     /locus_tag="ACHVB7_000149"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR1912260.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18424..19851
                     /gene="sbcB"
                     /locus_tag="ACHVB7_000150"
     CDS             18424..19851
                     /gene="sbcB"
                     /locus_tag="ACHVB7_000150"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR1912261.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(19894..20121)
                     /gene="tsuB"
                     /locus_tag="ACHVB7_000151"
     CDS             complement(19894..20121)
                     /gene="tsuB"
                     /locus_tag="ACHVB7_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR1912262.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20135..21193)
                     /gene="tsuA"
                     /locus_tag="ACHVB7_000152"
     CDS             complement(20135..21193)
                     /gene="tsuA"
                     /locus_tag="ACHVB7_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR1912263.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(21372..22730)
                     /gene="plaP"
                     /locus_tag="ACHVB7_000153"
     CDS             complement(21372..22730)
                     /gene="plaP"
                     /locus_tag="ACHVB7_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR1912264.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(22720..22782)
                     /gene="yoeI"
                     /locus_tag="ACHVB7_000154"
     CDS             complement(22720..22782)
                     /gene="yoeI"
                     /locus_tag="ACHVB7_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR1912265.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(22997..23926)
                     /locus_tag="ACHVB7_000155"
     CDS             complement(22997..23926)
                     /locus_tag="ACHVB7_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1912266.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(23972..24796)
                     /locus_tag="ACHVB7_000156"
     CDS             complement(23972..24796)
                     /locus_tag="ACHVB7_000156"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1912267.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(24879..25133)
                     /gene="yoeB"
                     /locus_tag="ACHVB7_000157"
     CDS             complement(24879..25133)
                     /gene="yoeB"
                     /locus_tag="ACHVB7_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR1912268.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(25130..25381)
                     /gene="yefM"
                     /locus_tag="ACHVB7_000158"
     CDS             complement(25130..25381)
                     /gene="yefM"
                     /locus_tag="ACHVB7_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR1912269.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            25664..25714
                     /gene="hisL"
                     /locus_tag="ACHVB7_000159"
     CDS             25664..25714
                     /gene="hisL"
                     /locus_tag="ACHVB7_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR1912270.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    25690..25813
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            25860..26759
                     /gene="hisG"
                     /locus_tag="ACHVB7_000160"
     CDS             25860..26759
                     /gene="hisG"
                     /locus_tag="ACHVB7_000160"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR1912271.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            26765..28069
                     /gene="hisD"
                     /locus_tag="ACHVB7_000161"
     CDS             26765..28069
                     /gene="hisD"
                     /locus_tag="ACHVB7_000161"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR1912272.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            28066..29136
                     /gene="hisC"
                     /locus_tag="ACHVB7_000162"
     CDS             28066..29136
                     /gene="hisC"
                     /locus_tag="ACHVB7_000162"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR1912273.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCESGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            29136..30203
                     /gene="hisB"
                     /locus_tag="ACHVB7_000163"
     CDS             29136..30203
                     /gene="hisB"
                     /locus_tag="ACHVB7_000163"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR1912274.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            30203..30793
                     /gene="hisH"
                     /locus_tag="ACHVB7_000164"
     CDS             30203..30793
                     /gene="hisH"
                     /locus_tag="ACHVB7_000164"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR1912275.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            30793..31530
                     /gene="hisA"
                     /locus_tag="ACHVB7_000165"
     CDS             30793..31530
                     /gene="hisA"
                     /locus_tag="ACHVB7_000165"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR1912276.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            31512..32288
                     /gene="hisF"
                     /locus_tag="ACHVB7_000166"
     CDS             31512..32288
                     /gene="hisF"
                     /locus_tag="ACHVB7_000166"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR1912277.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            32282..32893
                     /gene="hisIE"
                     /locus_tag="ACHVB7_000167"
     CDS             32282..32893
                     /gene="hisIE"
                     /locus_tag="ACHVB7_000167"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR1912278.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(32990..33967)
                     /gene="wzzB"
                     /locus_tag="ACHVB7_000168"
     CDS             complement(32990..33967)
                     /gene="wzzB"
                     /locus_tag="ACHVB7_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR1912279.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(34113..35279)
                     /gene="ugd"
                     /locus_tag="ACHVB7_000169"
     CDS             complement(34113..35279)
                     /gene="ugd"
                     /locus_tag="ACHVB7_000169"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR1912280.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(35529..36935)
                     /gene="gndA"
                     /locus_tag="ACHVB7_000170"
     CDS             complement(35529..36935)
                     /gene="gndA"
                     /locus_tag="ACHVB7_000170"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR1912281.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(37099..38469)
                     /gene="cpsG"
                     /locus_tag="ACHVB7_000171"
                     /gene_synonym="manB"
     CDS             complement(37099..38469)
                     /gene="cpsG"
                     /locus_tag="ACHVB7_000171"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR1912282.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(38574..40010)
                     /gene="cpsB"
                     /locus_tag="ACHVB7_000172"
     CDS             complement(38574..40010)
                     /gene="cpsB"
                     /locus_tag="ACHVB7_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR1912283.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(40013..41236)
                     /gene="wcaI"
                     /locus_tag="ACHVB7_000173"
     CDS             complement(40013..41236)
                     /gene="wcaI"
                     /locus_tag="ACHVB7_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR1912284.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(41233..41712)
                     /gene="gmm"
                     /locus_tag="ACHVB7_000174"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(41233..41712)
                     /gene="gmm"
                     /locus_tag="ACHVB7_000174"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR1912285.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(41715..42680)
                     /gene="fcl"
                     /locus_tag="ACHVB7_000175"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(41715..42680)
                     /gene="fcl"
                     /locus_tag="ACHVB7_000175"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR1912286.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(42683..43804)
                     /gene="gmd"
                     /locus_tag="ACHVB7_000176"
     CDS             complement(42683..43804)
                     /gene="gmd"
                     /locus_tag="ACHVB7_000176"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR1912287.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(43830..44378)
                     /gene="wcaF"
                     /locus_tag="ACHVB7_000177"
     CDS             complement(43830..44378)
                     /gene="wcaF"
                     /locus_tag="ACHVB7_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR1912288.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(44394..45140)
                     /gene="wcaE"
                     /locus_tag="ACHVB7_000178"
     CDS             complement(44394..45140)
                     /gene="wcaE"
                     /locus_tag="ACHVB7_000178"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR1912289.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(45151..46368)
                     /gene="wcaD"
                     /locus_tag="ACHVB7_000179"
     CDS             complement(45151..46368)
                     /gene="wcaD"
                     /locus_tag="ACHVB7_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR1912290.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(46343..47560)
                     /gene="wcaC"
                     /locus_tag="ACHVB7_000180"
     CDS             complement(46343..47560)
                     /gene="wcaC"
                     /locus_tag="ACHVB7_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR1912291.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(47557..48045)
                     /gene="wcaB"
                     /locus_tag="ACHVB7_000181"
     CDS             complement(47557..48045)
                     /gene="wcaB"
                     /locus_tag="ACHVB7_000181"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR1912292.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(48048..48887)
                     /gene="wcaA"
                     /locus_tag="ACHVB7_000182"
     CDS             complement(48048..48887)
                     /gene="wcaA"
                     /locus_tag="ACHVB7_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR1912293.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(48980..51142)
                     /gene="wzc"
                     /locus_tag="ACHVB7_000183"
     CDS             complement(48980..51142)
                     /gene="wzc"
                     /locus_tag="ACHVB7_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR1912294.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(51145..51588)
                     /gene="wzb"
                     /locus_tag="ACHVB7_000184"
     CDS             complement(51145..51588)
                     /gene="wzb"
                     /locus_tag="ACHVB7_000184"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR1912295.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(51594..52733)
                     /gene="wza"
                     /locus_tag="ACHVB7_000185"
     CDS             complement(51594..52733)
                     /gene="wza"
                     /locus_tag="ACHVB7_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR1912296.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            53042..53191
                     /locus_tag="ACHVB7_000186"
     CDS             53042..53191
                     /locus_tag="ACHVB7_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912297.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            53392..54975
                     /locus_tag="ACHVB7_000187"
     CDS             53392..54975
                     /locus_tag="ACHVB7_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1912298.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(55249..57102)
                     /gene="asmA"
                     /locus_tag="ACHVB7_000188"
     CDS             complement(55249..57102)
                     /gene="asmA"
                     /locus_tag="ACHVB7_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR1912299.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(57124..57705)
                     /gene="dcd"
                     /locus_tag="ACHVB7_000189"
     CDS             complement(57124..57705)
                     /gene="dcd"
                     /locus_tag="ACHVB7_000189"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR1912300.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(57797..58438)
                     /gene="udk"
                     /locus_tag="ACHVB7_000190"
     CDS             complement(57797..58438)
                     /gene="udk"
                     /locus_tag="ACHVB7_000190"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR1912301.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            58756..62073
                     /locus_tag="ACHVB7_000191"
     CDS             58756..62073
                     /locus_tag="ACHVB7_000191"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1912302.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(62183..63031)
                     /gene="alkA"
                     /locus_tag="ACHVB7_000192"
     CDS             complement(62183..63031)
                     /gene="alkA"
                     /locus_tag="ACHVB7_000192"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR1912303.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            63165..64517
                     /gene="yegD"
                     /locus_tag="ACHVB7_000193"
     CDS             63165..64517
                     /gene="yegD"
                     /locus_tag="ACHVB7_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1912304.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(64530..66470)
                     /gene="yegI"
                     /locus_tag="ACHVB7_000194"
     CDS             complement(64530..66470)
                     /gene="yegI"
                     /locus_tag="ACHVB7_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR1912305.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(66467..67228)
                     /gene="pphC"
                     /locus_tag="ACHVB7_000195"
     CDS             complement(66467..67228)
                     /gene="pphC"
                     /locus_tag="ACHVB7_000195"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR1912306.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(67225..67884)
                     /locus_tag="ACHVB7_000196"
     CDS             complement(67225..67884)
                     /locus_tag="ACHVB7_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1912307.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(68105..68164)
                     /gene="ibsA"
                     /locus_tag="ACHVB7_000197"
     CDS             complement(68105..68164)
                     /gene="ibsA"
                     /locus_tag="ACHVB7_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR1912308.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(68437..68493)
                     /locus_tag="ACHVB7_000198"
     CDS             complement(68437..68493)
                     /locus_tag="ACHVB7_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1912309.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(68764..68820)
                     /locus_tag="ACHVB7_000199"
     CDS             complement(68764..68820)
                     /locus_tag="ACHVB7_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1912310.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            69099..70340
                     /gene="mdtA"
                     /locus_tag="ACHVB7_000200"
     CDS             69099..70340
                     /gene="mdtA"
                     /locus_tag="ACHVB7_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR1912311.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            70340..73462
                     /gene="mdtB"
                     /locus_tag="ACHVB7_000201"
     CDS             70340..73462
                     /gene="mdtB"
                     /locus_tag="ACHVB7_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR1912312.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            73463..76540
                     /gene="mdtC"
                     /locus_tag="ACHVB7_000202"
     CDS             73463..76540
                     /gene="mdtC"
                     /locus_tag="ACHVB7_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR1912313.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            76541..77956
                     /locus_tag="ACHVB7_000203"
     CDS             76541..77956
                     /locus_tag="ACHVB7_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1912314.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            77953..79356
                     /gene="baeS"
                     /locus_tag="ACHVB7_000204"
     CDS             77953..79356
                     /gene="baeS"
                     /locus_tag="ACHVB7_000204"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR1912315.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            79353..80075
                     /gene="baeR"
                     /locus_tag="ACHVB7_000205"
     CDS             79353..80075
                     /gene="baeR"
                     /locus_tag="ACHVB7_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR1912316.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        19328 a        21222 c        20477 g        19131 t
ORIGIN      
        1 cagccattct ttttcctcgc ttttttttat agtcatatcg tcaaaaaaat ccaggcgagg
       61 tctttatgca acaagaagcg ttaggaatgg tagaaaccaa aggcttaact gccgccatag
      121 aggccgcaga cgccatggtg aagtcggcaa atgttttgct ggtcggctac gaaaaaatag
      181 gttccgggct ggtgaccgtc atcgttcgtg gtgatgtcgg ggcggtaaaa gcggcaacag
      241 acgcgggtgc cgcagctgcg cataacgtcg gcgaagtaaa agccgtacac gttattcccc
      301 gcccacatac cgatgtcgaa aaaatcttac caaagggaat taacccatga gcagcaatga
      361 actggtcgaa cagatcatgg cgcaggtcat cgctcgcgta gcgacgccag agcatgaggt
      421 tctcccgaaa accactcatc aaatacgaga gacggctatg gcagaaaaaa gctgcacgct
      481 aacagaattt gttggcaccg ctattggcga cactgtaggt ctggtcattg cgaacgttga
      541 tagcgccctg ttggacgcga tgaaacttga aaagcgctat cgttccattg gcattctggg
      601 tgcgcgtatt ggcgcagggc cacacattat ggccgctgat gaagcggtaa aagccaccaa
      661 cactgaagtt attagcattg aacttcctcg cgatactaaa ggcggtgccg gacacggctc
      721 actgatcctc ttaggtggca acgatgtttc cgacgtgaaa cgcggtattg aagtcgccct
      781 gaaagaactc gatcggacct ttggcgatgt ctatgccaac gaagccggac atcttgaact
      841 gcaatatacc gcccgcgcca gctatgcact ggaaaaagcg tttggtgcac cactgggacg
      901 ggcttgcggc gttattgtcg gcgctccggc tgctgtgggt gttctgatgg ccgacaccgc
      961 gctgaagtcc gccaatgtgg atgttgtggc atacagctcc ccagctcaag acaccagcta
     1021 cagcaatgaa gtcattctga ttatctcggg tgactccggc gcagtccgcc aggcggtcat
     1081 ctccgcacgc gaaatcggta aaaccgtgct ttcgactctc ggcgatacac cgaaaaacga
     1141 tcacccttcc tacatctgaa ttctctgagg cagatttatg agatcgaaaa gatttgaagc
     1201 actggcaaaa cgcccggtca accaggacgg tttcgttaaa gagtggattg aagaaggctt
     1261 tattgcgatg gaaagcccga acgatcctaa accgtcaatc aaaattgtta acggcgtcgt
     1321 taccgaactg gatggaaaac ccgccagcca gtttgacctg atcgaccact ttatcgcccg
     1381 ctacggcatc aatctcgcgc gcgccgaaga agtcatcgcg atggattcgg taaaacttgc
     1441 caatatgttg tgcgatccaa atgtcaaacg tagcgatatc gttcccctta ccacagcaat
     1501 gacgcctgca aaaatcgtcg aagtggtttc gcagatgaac gtggtagaga tgatgatggc
     1561 gatgcagaaa atgcgcgccc gtcgaacacc atcacaacag gctcacgtca ccaacgtcaa
     1621 agataaccca gtacaaattg ccgccgacgc agctgaaggc gcatggcgcg ggtttgatga
     1681 acaggaaacc accgttgccg ttgctcgcta tgcaccgttc aacgccatcg ccctgctggt
     1741 cggctcacag gtgggacgtc cgggcgtctt gacccagtgt tcactggaag aagccaccga
     1801 actgaaactc ggtatgttag gccacacctg ttacgccgaa acaatttctg tctatggtac
     1861 agaacctgtt ttcaccaatg gtgatgatac cccgtggtca aaagggtttc tcgcgtcatc
     1921 ctatgcttct cgtggcttaa aaatgcgctt tacttccggc tccggctctg aagtacagat
     1981 gggttacgcg gaaggcaaat caatgcttta cctcgaggct cgttgcattt acatcaccaa
     2041 agccgcaggc gttcagggtc tgcagaatgg ttcggtaagc tgcatcggtg ttccatcagc
     2101 tgtgccatca ggcatccgcg cagtattagc ggaaaactta atttgctcat cgctggatct
     2161 ggaatgcgcc tccagtaatg accagacctt tacccactcg gatatgcgcc gtaccgcacg
     2221 tttcctgatg caatttctgc caggcaccga ctttatctct tccggttttt ccgctgtgcc
     2281 gaactacgac aacatgtttg ctggttcaaa cgaagatgct gaagacttcg atgattacaa
     2341 cgtcattcag cgtgacctga aggttgatgg tgggctgcga ccagtacgcg aagaagatgt
     2401 tatcgctatc cgcaataaag ccgctcgcgc tttacaggct gtctttgccg gtatgggact
     2461 accgcctatt accgatgaag aagtcgaagc ggcaacctat gcccacggtt caaaagatat
     2521 gcctgagcgc aatattgtgg aagacatcaa gttcgcccag gagattatca ataaaaaccg
     2581 gaacagcctg gaagtggtga aagccttggc gcaaggcagt tttaccgatg tcgcccagga
     2641 catgctcaac atgcaaaaag caaaactgac cggcgattat ctccatacct cagccatcat
     2701 cgtcgatgac ggacaagtgc tctctgcggt caatgacgtc aatgattacg ccggaccggc
     2761 tacaggttac cgcctgcaag gggaacgctg ggaagagatc aaaaatattc ccggtgcact
     2821 tgatcccaac gaaattgact aagggtgaga aatggaaatt aacgaaaaat tactgcgtca
     2881 gattatcgaa gacgtgctgg cagaaatgca acccagcgac aagtccgttt catttcgcgc
     2941 acctgtctct gcaactgtcc cttccgcgcc agataccgga aattttctga ccgaaattgg
     3001 cgaagcccaa caaggcactc agcaagatga agtcattatt gctgtaggcc cggcgttcgg
     3061 cctggcgcaa accgtcaata tcatcggtat tccgcataaa aacatcttgc gcgaagtgat
     3121 tgccggtatc gaagaagaag gcattaaagc ccgtgtaatt cgctgcttta aatcgtccga
     3181 tgtcgcattc gtagcagtcg aaggcgatcg tcttagtggt tcaggtattg ctatcggtat
     3241 tcagtcgaaa ggcacaacgg ttatccacca gcagggactt cctcccttgt ctaacctgga
     3301 acttttccca caagcgcctt tgttgacgct ggatacttat cgtcagattg gcaaaaacgc
     3361 cgcgcgttat gcgaagcgcg agtctccaca accggtgccg accctcaacg atcaaatggc
     3421 gcggccgaaa taccaggcca aatctgccat tttgcatatt aaagagacga agtacgtggt
     3481 cactggcaaa aaaccgcaag aactgcgcgt gacgttttaa caaaggaaac cttcatgaat
     3541 accgatgcaa ttgagtcgat ggtcagggac gtgttgaacc gcatgaacag tttacaggat
     3601 gcagctcctg tttctgcggt ccccaacgca tcaatactga gcgccaaagt gacggattac
     3661 ccgctggcaa ataaacatcc ggaatgggtt aaaacagcga ccaataaaac gctcgacgat
     3721 ttcactctgg aaaatgtgct aagcgataac gtcaccgccc tggatatgcg cattacccct
     3781 gaaacattgc gtattcaggc tgcgattgcc agagatgctg gtcgcgatcg tctggcgatg
     3841 aattttgaac gcgccgctga gcttacctca gtaccagatg atcgcatcct cgaaatctac
     3901 aatgcgttac gcccttatcg ttcaactaag caagaactta ttgccattgc tgacgatctt
     3961 gaacagcgtt atcaggccaa aatttgtgct gcatttgtcc gtgaagctgc ggaactttat
     4021 gtcgagcgca aaaaactgaa aggcgacgat taaggacgaa ctatgcgata tatagcaggc
     4081 attgatatcg gcaactcatc aacagaggtt gcgctggcaa cgctcagtgc aactggagag
     4141 ttgtcgttcg tgagtagcgc cctggaggaa accaccggaa tcaaaggtac gttgcgtaac
     4201 gtccatggta ttcaggaggc gctcgcacag gcaacgaaaa aagtcggcat caatgttagc
     4261 gatatttcgc ttattcgtat caacgaagca actccggtca ttggtgatgt ggcgatggaa
     4321 accatcactg aaactatcat caccgaatca accatgatcg gtcacaaccc gaaaactccg
     4381 ggcggagtgg ggctgggcgt agggttcacc atcactccgc aagagttatt aacacgcccg
     4441 gcggacacac cttacatcct ggtggtgtca tcggcatttg attttgccga tatcgcgacg
     4501 atgataaacg cctccgtacg cgccggatat cagctgactg gcgttattct gcaacgcgac
     4561 gatggcgtac tggtcaataa ccgcctggaa attcctttac ctattgtgga tgaagtcctc
     4621 tacatcgatc gtattccgct cggcatgctg gcagcaattg aagttgccgt tcccgggaaa
     4681 gttatcgaaa ccctgtctaa cccgtatggg attgccaccg tattcgccct caatgctgaa
     4741 gagactaaaa atattgttcc tgtcgcgcgg gcgctaatcg gcaaccgttc agcagtggta
     4801 gtgaaaacgc cctctggcga cgttaaagcc cgttcgatac ccgccggaaa tatcgaactg
     4861 ctttctgccg ggcgcacaac gcgcgttgat gtagctgctg gtgccgatgc cattatgaaa
     4921 gcggtcggtg agtgcccaaa actggaaaac gtgacgggag aaccaggcac caacatcggc
     4981 ggaatgctgg aacatgttcg ccagaccatg gcggaactga ccaacaaacc cagcaacgaa
     5041 atttttattc aggatttgct ggcgatagat acctctgtcc ccgtcagcgt aaccggtggt
     5101 ctggcaggtg aattttcact ggaacaggct gtgggtattg cctctatggt gaaatccgac
     5161 cgtctgcaaa tggcgatgat cgccagtgaa attaagcaaa aactgcatgt tgatgttcag
     5221 gtcggaggcg cagaagcaga agccgccatt cagggggcct tgactacccc tggaaccaca
     5281 cgtccactgg caattctgga cttaggtgca ggctctaccg atgcctccat catcaatcag
     5341 agtggggaaa tcgttgccac tcaccttgcg ggagcgggcg acatggtaac gatgattatc
     5401 gctcgtgagc tgggcttaaa tgatcgttat ctggcagaag aaatcaaaaa gtacccgtta
     5461 gcaaaagtag aaagcctgtt ccacttacgc catgaagacg gtagcgttca gttcttccct
     5521 actcccctat ccccccatgt atttgcccgc gtctgtgtag tgaaacccga cgaactggtg
     5581 cctattcccg gtgatttgac gctggaaaaa gtacgcgctg ttcgtcgcag tgcgaaagag
     5641 cgtgtcttcg taaccaatgc cctgcgcgcg ctacgtcagg taagccctgc cggaaatatt
     5701 cgcgacattc cgttcgtcgt tctggtgggt ggttcttcgc tggatttcga agtaccgcaa
     5761 ttggtcaccg acgcgctggc acattatcga ctggtcgccg ggcgggggaa tatccgtggt
     5821 agcgaaggtc cgcgcaacgc ggtagctact gggctactca tcgcctggca caaggagtcg
     5881 attcatggaa agtaaagagc gtgcaccagc catcgttgtc atggagatcg gtgactgtat
     5941 cactacatgg gatgaggtgc ttcttggcat tgaagaggaa ggtattccct ttcgcattca
     6001 acacattccg tcaggtgaag ttatcgatag cgcctggctg gctgctcgtc aatctcccct
     6061 gttggttggc attgcctgcg accgggaaga actgattgtg cattacaaaa acttaccggc
     6121 atcagcgcca ctgtttacgc tgacgtatca acaagataac catacccggc gcagcatcgg
     6181 cactaacgct gcccgcctgg taaaaggaat tcccttccgg gaattccact cataatccac
     6241 aggagaaacg ctgtatgaac aacgcactag gactggttga aacaaaaggg ttagttggcg
     6301 caattgaagc tgcagatgcc atggttaaat ccgccaacgt gcagctggtt ggctatgaaa
     6361 agattggttc gggtctgatt acggtgatgg tccgcggtga tgttggcgca gtaaaggccg
     6421 ccgttgatgc ggggagcgct gcggcaagcg ccgttggcga agtgaaatcc tgccatgtca
     6481 tcccgcgccc acacagtgat gttgaggcta ttttacctaa atcggcataa ccccatttgc
     6541 taaggagcac agcgtgaagc aatcactggg attacttgaa gccagtggtc tggctctcgc
     6601 catcagttgc gcagacgcta tggcaaaagc cgcgtccatc acgctggttg gccttgagaa
     6661 gaccaatggc tcaggctgga cgctcatcaa aatcaccggt gacgtcgcct cggttaatgc
     6721 ggcgatcgcg acgggagcca gtttcgccgg acagcgaaat ggccttgtcg cacacaaagt
     6781 gattgcccgc ccgggagacg gcattctgcc ccacacaact gaagcattga ccgtgacggt
     6841 tgaaacaagc aaaacaccag caacaaccgt cacctcagag attatcagtt gtaatttgtg
     6901 tcttgacccg gcatgtcagc ggcaaaaagg tgagccccgc tcactttgcc tgtacgccaa
     6961 taagcgaggg gagtagcagt ggataaacaa accttgcagg aaacagtgag ccagattctg
     7021 gatgagatgc gcgagcgccc tatcccactg ggcgtttcca gtcgccatat acatctttca
     7081 tcagcggatt atttgcgtct ttttcctgaa caaccgatta gcgagaaaaa gccgttaatg
     7141 caacctggcc aatacgctgc tgaacaggtt gttacgctgg agggaccaaa aggcaaattg
     7201 cacaacgttc gcttgttggg gccgctacgc aatgcaagcc aggtggaaat ttcccggacg
     7261 gacgccagaa cgttagggat caccgccccg ctacgcatgt caggcgatct cagcggcact
     7321 ccggggattc gtttgataag cccatttgcg gaactggaga tcacatctgg cgtcattgtc
     7381 gcgcaacggc atattcatat gtctccgctg gatgccctga tttatggcgt tacgcatggc
     7441 gataaagtgg cggttgccat tgaaggtgat gagcgacgac tgatattcga aaacgtcgct
     7501 gttcgcgtct ctccggatat gcgtcttgaa atgcatattg ataccgatga ggccaacgcc
     7561 gcaggcgctg atagccctga cgtttttgcc acactggtgt cagtgccatg aataacatgc
     7621 tgatgcaacg tattgttgac gaagtggttt ttcgccttaa acaacgtgcg ggaaaaacac
     7681 tcgtgctgac tgtttttcaa ttgcgagacg ccagcgttca agagagcgtt catcagtatg
     7741 cgtcattaca aatccgatat gtcgatctgc cgctgctgcg acaacttgcg gagaacgaaa
     7801 cgtcagatcg agcatctatc cagatacacg aagcgttagc ctggggaatg cacattcaat
     7861 tgtcgttgca gcgtcatttc ctgaacgcca tcgaattgaa gactctcgcc cgtctaccgc
     7921 taagctggtg cgacgaacaa gggcagccga tctaccttca tcataatcga cttttaagtt
     7981 atgccgatat cgcacagtta agctcgggaa tactggtgtt acagcggaaa tgttgtgtga
     8041 ccgcactggc tcgtgaagcg gcaattaccc gaaatattca gttaatcagg caggagtaac
     8101 ctatgcatct ggctcgagtc actggcgcgg tagtatccac gcaaaaatca ccttctctga
     8161 tcgggaaaaa actgctgctg gttcgccggg tcagtgctga cggcgaactg cctctcacgc
     8221 cattaaccgg cgacgaagtt gccgttgatt cagtcggcgc aggcgttggt gagttggtac
     8281 tcctcagcag tggttccagc gccagacatg tgttttcagg ccctaacgag gccatcgacc
     8341 tggcagtggt cggcattgtc gatacgcttt cgcgctaagg agctaaaaga tggcaattta
     8401 tacccgaaca ggtgattcag gaagtacgtc tttatttacc ggacagcgcg tcagcaaaac
     8461 tcatctacgt gtcgaaacct atggcacgct ggatgaactg aacgcgacac tcagtctgtg
     8521 ttattgcgcc accgccatcg aaagccatcg catcttactg gaagccattc aacaacagat
     8581 attttggttt agcgccgaac tcgccagtga aagcgaacag ccgtcagcgc aacagcgtta
     8641 catcggtacg gaagaaattg ccgcgttgga aaacgcgatc gatagtgcaa tgaacgccgt
     8701 ccccccagtt cacagcttca ttctgcctgg tcgatgtgaa gccgcaagcc gcatgcattt
     8761 cgcccgcacg gtggctcgac gggctgaaag acggctggtt gaactgacaa cggaaaccac
     8821 cgtccggaat gttttgctgc actatatcaa ccgactttcc gattgcctgt atgccctcgc
     8881 ccgcgtggaa gacaacgttg ctcatcaaaa cctgatgatt caggaaatca caaaacgtta
     8941 tcacgaagcc aaccacacac cagcattgaa ggaacgcaca atgccgctca cttttcagga
     9001 tcttcaccag ctcattcgta gtgcggcaat gcgcgcggat gaactgcata ttcccgttgt
     9061 catcagcatt gttgatgcca acggaacaga aagtgttacc tggcggatgc ctgatgccct
     9121 gttggtcagc agtgaactgg caccgaaaaa agcctggacc gccgtggcga tgaaaacggc
     9181 aacccacaag cttgctgata ccgtgcaacc cgacgcgccg ctttacgggc tcgaaagcca
     9241 tatgcaagga aaagtcgtca cttttggtgg cggcttcccc ctctggcgtg acggaaaatt
     9301 gcttggcggg cttggcatca gcggcggtag cgttgaacaa gacatggata ttgcccaaag
     9361 cgcaatggcg gcaattaacg tgggagtaaa ccaatgaata actctgaatt agaaacactg
     9421 atccgcaata ttctgcgtga gcaacttgtc cccgctacac cacgaacgca aagaaacgct
     9481 attttccaga ctgtcgatga agcggtctgc gcagcacact aggccttttt acgctttcaa
     9541 cagtgcccgc ttaaaacccg tagcgccatt atcagcgcca ttcgcgaaga attaatgccc
     9601 cgccttaccg aactggcaga agaaagcgcc aaagaaacgg ggatgggaaa taaagaagat
     9661 aagtacctaa aaaacaaagc ggcactcgat aacacgccag gaattgaaga tttaaccacc
     9721 acagcactaa ccggtgatgg tggcatggtt ttgtttgagt actctccgtt tggggtgatt
     9781 ggctcggtca cgccaagcac caaccccacg gaaaccatca ttaacaacag catcagcatg
     9841 ctggcagcag gtaatagcgt ctacttcagt ccacacccgg gtgccaaaaa cgtttcgtta
     9901 aaactgatta ccacaatcga agacatcgcg tttcgctgta gtggcattca taacttgatc
     9961 gttaccgtat ctgaaccgac ctttgctgcc accgaacaga tgatggcgca ccctaaaatc
    10021 agcgttctgg cgattaccgg cggtccgggg atcgtcgcca tgggaatgaa aagtggcaag
    10081 aaagtgattg gtgccggagc gggtaaccct ccgtgcatcg tcgatgaaac ggcggatatt
    10141 gtgaaagccg ccgaggacat catcaacggc gcttcatttg attacaacct gccgtgtatt
    10201 gctgaaaaaa gcctgattgt cgtggcatca gtggcagatt atttaattca gcagatgcaa
    10261 agttttggcg cacttcttct taactatgag caaactgaga aattgcgtgc aatttgcctg
    10321 ccagacggta gcgccaacaa aaaactggtt gggaaaagcc catcagcctt gctggaagcg
    10381 gcaggcctcc ctttgcccgc aaaagcgccg cgcctactga ttgctgttgt cgacgccgat
    10441 gatagttggg ttacttgtga gcaactaatg ccaatgctgc ccattgtgaa ggtcaaggat
    10501 tttgacagcg ctctaaccct ggcgctgaaa gtggaagacg gcttgcacca cacggcaatt
    10561 atgcactctc agaacgtctc acgactcaac cttgccgcgc gcgttatgca aacctcaata
    10621 tttgtcaaaa acggcccctc ctgggcgggt attggcgtcg gcggcgaagg ctttacgacc
    10681 tttaccattg caacaccgac tggagagggt acaacgtcag cacggacttt tgcccgctca
    10741 cgacgctgcg tgttaaccag cggcttttca attcgctaga gaaacggaaa tgaatacttt
    10801 ttcactgcag acgcgtttat acagcggacc aggaagtctc aaggtactta gccgtttctc
    10861 tcatagacac atctggatta tttgcgatgc ctttctggca cgctcaccac ttattgacac
    10921 gttgcatcag gcgctgcccg acagtaatcg tctcagtatt tttagcgaaa taacgcccgc
    10981 cccaactatc cagaccgtgg tgaaaggcat tgcgcaaatg cagactttac gcccggatgt
    11041 agttatcggt tttggcggcg gttctgccct ggacgccgca aaagcaattg tctggttcgg
    11101 tcgccagtgt ggcattgaaa ttgaaacctg cgtagccatt cctaccacca gcggtatcgg
    11161 ttccgaagtg accagtgctt gtgtcatcag cgatcctgag aaagggatta aatatcccct
    11221 tttcgataat gcactttatc cggatatcgc gatccttgat ccatctttgg tcgtcagcgt
    11281 gccaccggcg ataacggcca ataccgggat ggatgtttta acccatgcgc ttgaagctta
    11341 tgtttctcct cgcgccagcg acttcacgga tgcactggtc gaaaaagccg tgcaaattgt
    11401 ttttcaatac ctgcccactg cagtgaaaaa aggcgattgt cttgccaccc gtggcaaaat
    11461 gcataatgca tcgacgcttg caggaatggc ctttagccag gccggactcg gtttaaacca
    11521 tgcaattgct catcaactcg gcggacagtt tcatctgccg catggactgg cgaatgcgct
    11581 cctgctcacg gcagttatcc gctttaatgc tggcgatccg cgcacagcca aacgctatgc
    11641 ccggctggcg aaaacgtgcc atttatgccc ggacaacgcc aatgacaccg ccagcctgaa
    11701 tgcacttatt caacatattg agcagcttaa tacaacctgt acgctgccga ctcttgccaa
    11761 tgcgctgaaa gaaaaaaaag cagaatggtc aatacgcata ccagatatgg ttcaggcggc
    11821 gctggcggat gcaacgttgc gtaccaaccc gcgtgcagct gatgcctccg cgattgcaga
    11881 actgctcaag gggttgttat gaacgtcgga ttaatctcct gcgacgacca gaccatccgc
    11941 gagcgagtac aagcagcagg tgtcgtgggt gctggcggcg caggttttcc aacccatatt
    12001 aagctacaag cgcgtgctga cactttcctg gtgaatgcgg cggaatgcga acccatgttg
    12061 aaagtagatc aacagttaat ggcgcagcaa gccagccgcc tgcttcgcgg tgttcactat
    12121 gcgatgaaag ccaccggggc cagtagcgga atcatcgcgc tgaaagaaaa atatcaacgg
    12181 gcaataaacg cactcacacc actgttacca ccggatatcc gcctgcatat tttgcccgac
    12241 gtttaccccg caggagacga agtgctgacc atctggatgg caactggtcg ccgcgttcca
    12301 cccgctgcat tacccgttag cgtcggtgtg gtggtcaata atgttcaaac cgtacttaat
    12361 attgccagag ccgtcgagca acagtatcca gtgacgcgcc gcacactcac cgttaatggt
    12421 gcagtggcga agccaataac ggtaaccgtc cccatcggca tgtctttacg tgaagtcctg
    12481 gcgctggcgg gcggcgcaac agtggatgct cctggtttta tcaatggcgg gccgatgatg
    12541 ggaagtttga tcacatcgct cgacacacca gtcagcaaaa ccactggcgg cttgctggtt
    12601 ttacccaact ctcattcact gatccaacgt cgaatgcaga atgaccgcag cgtacttgcc
    12661 gtcgcgaaaa ctgtttgtga gcaatgccgc ttgtgtaccg atctctgccc acgtcattta
    12721 atcggtcacg aactggcacc ccatttatta gtacgtgcgc ggttaactac caacagctcg
    12781 ccacgccgca actgttactg acagcgctca cctgttcaga gtgcaacgtc tgcgccagtg
    12841 tcgcctgtcc ggtaggaatt tcaccaatgc gcataaaccg cctgcttaag caggaattac
    12901 gcgcacaaaa tctccgctac gaaggtgcct taaatcctgc agatcccatg gcaaattatc
    12961 gactgattcc cgtaaaacgg ctggtaacca gacttggctt aaccccgtgg tatcaggatg
    13021 cgccattgtc tgaacaggtg cctcagcccg aaaaagtgac gttattgtta cgccagcaca
    13081 tcggtgccag cgcaattgcg tgtgtacaaa aaggcgatcg cgtcgttcat gggcaatgtg
    13141 taggtcaaat tccacacggc acattaggtg cccccattca tgccagtatt gatggcatgg
    13201 tcagcgatgt gacagaaaac gcgattacgc ttgtgagagg ttaaccatgt cgaaagcggt
    13261 aggtatttta gaacttagca gtatcgcgaa agggatggaa accggcgacg ccatgctgaa
    13321 aagcgccaac gttgagttac tggtcagcaa aacgctatgc cccggaaaat ttctgctgat
    13381 gctcggtggc gatgtgggcg cggtgcaaca ggccatcaac agcggagcgt cccttgctgg
    13441 tgaaatgctg gtcgacagtc ttgtcctgcc gaatattcat cccagcctcc tttctgcaat
    13501 cagcggactt aaccatgttg aacagcaccg ggctgtcggc gtagtcgaaa cctggagtgt
    13561 cgccgcctgc attgacgctg ccgattgtgc tgtcaaagct gcaaatgtca ttcttgttcg
    13621 cgtacatatg gcttttggca ttggcggtaa atgctacatg gtggttgctg gcgatatttc
    13681 agatgttaat aacgcggtct ctgccgccag cgtgcgcgcg ggggaaaaag gtttgttggt
    13741 ctatcggtcg gtgattgccc gtccacatga agccatgtgg cgtcagatgg tggagggata
    13801 atgacaactc agcacacaac tgaacggatg attcaggaat atgtcccagg taagcaggtc
    13861 acgctggcac atgtcatcgc caatccaggg aaggatctgt ttaagaaatt gggtttacca
    13921 gacaccattt ccgctattgg tattctgaca attaccccca gcgaagcatc tatcatcgca
    13981 tgcgatatcg ccacaaaatc aggcgctgta gagatcggtt ttcttgatcg ttttaccggt
    14041 gccgtagtgc tgactggtga tgtttctgcg atcgaatatg cacttagcca ggttacccgt
    14101 acgctgggtg aaatgatgcg ctttaccgcc tgccccatta cgcggacctg acgttatgca
    14161 acgtatgatg ctcatcggcc ccagccagtg cggaaaaacg tcactcacgc aatgtctgcg
    14221 tggcgaaaat attcattatc agaaaacgca ggcaatcgtc tggtccccga ccaccattga
    14281 tacaccaggc gaatacctcg aaaaccgcag cctctacagt gcactgttgg tcagtgcctg
    14341 cgaagcggac attatcgcgc tggtgcttaa tgcggacgca ccgtggtcac ctttttcgcc
    14401 aggctttacc tcgtcaatga atcgcccggt cattggagta gtaaccaaag cagatttagc
    14461 caacatggaa caaatatctt tggtgaaatg ttggttacgg gaagctggag cgcacaacgt
    14521 gttagtaacc agtgcggtta ataaccatgg agttgcagaa cttttagcct tgctgcatac
    14581 agaagaaggc tgtcgctaat ttcgacaggt tatcagatgg gaatgatgac ggtgatacac
    14641 agaaaagaat caatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacaggaat
    14701 tacttcgctt cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg
    14761 cgttctttgg agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca
    14821 cctttcatgc tggcgatgat gccctggatt gcttcaacgc tcatcccagg cttgatgtaa
    14881 tcgctcaggt tgtccagcag caggacgcgc ttctggttgt cacggatctt tttctcaacg
    14941 tcctgaattt cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg
    15001 tcctgaaatg aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat
    15061 tcgtttaagc aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa
    15121 tgcttttatc ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag
    15181 atgattctga agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac
    15241 tcaagctgat gcgccgtttc catgttcagc cacacataac catagattgg tgtttccact
    15301 accttcaggt catggtggtt attgagcaac tgacgcagct cttccacagc atcgttcaat
    15361 ttttccgtat tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaagctc
    15421 agcagtattt gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg
    15481 aaatggctgg ggcgcgtggc ccagtacgca ttgatttgca actccagcat acaaaccaga
    15541 ttgcggttaa tggtctggat accttcatat atcgattttg gaatacgggt ttctttgctg
    15601 gcgggcgcaa tcagcccacg cattttcacg gcatcggtca ggagtttttg tagatggctt
    15661 tccagacgtg ggcgttcgag taagttcggt gagaatgcag attgatagac ccgattatac
    15721 tcggtcagac ttttcgccag ttgaatgcgc cagtggatga acgcccgttg tggccagata
    15781 ccggtaaaca acattgccag taaagagccg aggatcacat cgccgcttcg ccataacgcc
    15841 gtatcaattt cacctgtcgg ggaaccaaca acaattgcca gcgtcacccc aatcaataat
    15901 ccttgatacg gtttcttgcc cagcgccagc caaccgcaaa gaaacatcgc cgccgcgcac
    15961 cagactaaca tcagcggtaa cgaaattaac tccagttgca gagcgataag acctaaaatc
    16021 gaacccaaca ccgtaccgcc aatacgttcg aaggcacgag ggacaacgtt accccagaac
    16081 gagattggcc ccataatcac caccatggtg accagcggcc aggtgctttc cgggatagta
    16141 aacaggcgga taatgagaaa agtgagcagg aatgccagcg cgacccgagt accatgcact
    16201 atgcggtaat ggcggtatac ccggatttca aacgggctta atgacttatc ggcacgcacg
    16261 cagaaactcc gtaatgaaga ttaaaagcgc tcataactca tctgtcggcc agaaggcgcg
    16321 cctctgaatt atccgacgga caaaccggga acagtgtaat ttcccggcag gagcgaaaaa
    16381 attgaaaggc gcatcttact cttttcgctt tcaaaaaaag agtggtcatc gcgttaacac
    16441 accgccctga gatgaattag tgatgttttg gctgcaccgc aacatacatt tcgatatccc
    16501 agtacccatc ttccgcgcca ttgttcaaat aaacctcgaa gcagggcttt ggtaacattt
    16561 cataagcact gtcctgcaag aggctattaa agaactgata ccagggttta gcaaaatcat
    16621 caccgactac acgagccacc gccaccgcat actgaccacc tgaaatttct gtcagaatga
    16681 cgccctcact gttttcggga agcgtaaagt tattcggtac cgtcacaacg gtgtcgcagc
    16741 gtaatttttc ggcgggtgtt tcatctggat tgtcgtaata gacagcaacc cactccttcg
    16801 gcacaatatt tttgttatct acccacatca tcaactgctc aaagcctttc tttaccgtct
    16861 gttcccacgg gccaacgaga tggaaacctg caacggtacg tttctcttcc tgcgtaatct
    16921 cgtagttcat gacgcctcca ttaacactgt ttttatatac agcatagttg caatattaaa
    16981 accacaagga atgagtgttg attatgcgag cagactcgca ctcctgccag tctgctgcaa
    17041 aagaaaggtc aggccttatg gtggaaataa tcactcaggc gggaaaacat gccgccttcc
    17101 ccgacagatt ccagggtaac cagcggccag tgcgccacct gtttatcacg gtcgtaaagt
    17161 tcaatttccc ctacccgctg atgggcgcta attggcgcgg tgagctcttt accatcaagg
    17221 gtatatttgg ctttgatatg tggaatttcg gctttcggta gcaccatcca gaactcttgt
    17281 tccgttccta ggtcgatatt ttctttatcg ccataccaga tgcgttccgt tccgaccttt
    17341 ttcccacggt gcaaaatttg caccgtagta aagttttgtt gcccccaacg cagtaatttt
    17401 cttgcctctt cctcacgacc ttttgcactg tcagccccca ttaccactgc aatgagacga
    17461 cgctgcccat ctacagccga agcaatgaga ttaaacccgg caccagaagt atgacccgtt
    17521 ttcaggccgt caacattcat ggttttatcc cacaacaacc cgttacggtt ttgctgggtg
    17581 ataccgttcc aggtgagact tttctcactg tacatatgat aaaactcggg ctcgccgtgg
    17641 atgatagcgc gagaaaacac agctaaatca taagccgagc tatgctggcc aggtgcatcc
    17701 agaccatgca ctgtttcaaa atgcgtatcc ttgagatgca gcttctcggc atagttgttc
    17761 atcatttcaa caaactgccg ttgcccaccg gcaatatagt cagccagagc aacacaagcg
    17821 tcatttccgg aatccacaat taaaccacgg cttaaatcac gtaccgatac gcgatcgccc
    17881 tctttcaaaa acatcagtga agaaccgaca aacaccggat tatctttcgc ccacgcatcg
    17941 cgccccacgg tgacaatatc gtctggcgta atgcgatgac tatcgatagc gcgatccacg
    18001 acataacccg tcatcagctt tgtcaggctg gcgggattgc gctgttgatg ctcattaccc
    18061 gcggtgagga tctgaccggt ggtgtaatcc atcaataccc aggacccggc atgaatctct
    18121 ggaggctgag gtgaaaaagg aatgttttcc gccgcaaaac cagacgataa gttaaaaacg
    18181 aacaaagaag cagcaataat aagacggcgt ttcaacagca aaccctcagg agtttcaaat
    18241 agctgttctt tttacggaaa tacttatgaa ctggctggaa taaagtgcaa gaaaatgtga
    18301 ctaccctctc atttttatct gacatgatct gttgccactc gctgccaaat tgtggcgcta
    18361 aagctgatta gcacggtgat atttgatact ctggcagaca gcagaaataa cggatttaac
    18421 ctaatgatga atgacggtaa gcaacaatct acctttttgt ttcacgatta cgaaaccttt
    18481 ggcacgcacc ccgcgttaga tcgccctgca cagttcgcag ccattcgcac cgataacgaa
    18541 ttcaatgtca tcggcgaacc cgaagtcttt tactgcaagc ccgctgatga ctatttaccc
    18601 cagccaggag ccgtattaat taccggtatt accccgcagg aagcacgggc gaaaggagaa
    18661 aacgaagccg cgtttgccgc ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt
    18721 ctgggctaca acaatgtgcg tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat
    18781 ttctacgatc cttacgcctg gagctggcag catgataact cgcgctggga tttgctggat
    18841 gttatgcgtg cctgttatgc cctgcgcccg gaaggaataa actggcctga aaatgatgac
    18901 ggtctaccga gctttcgcct tgagcattta accaaagcga atggaattga acatagcaac
    18961 gcccacgatg cgatggctga tgtgtacgcc actattgcga tggcgaaact ggtaaaaacg
    19021 cgtcagccac gtctgtttga ttatctcttt acccatcgta ataaacacaa actgatggcg
    19081 ttgattgatg ttccgcagat gaaacccctg gttcacgttt ccggaatgtt tggggcatgg
    19141 cgcggcaata ccagctgggt ggcaccgctg gcgtggcatc cagaaaatcg caatgccgta
    19201 attatggtgg atttggcagg agacatttcg ccattactgg aactggatag cgacacattg
    19261 cgcgagcgtt tatatactgc aaaagccgat cttggcgata acgccgccgt tccggttaag
    19321 ctggtgcata tcaataaatg tccggtgctg gcccaggcga atacgctacg cccggaagat
    19381 gccgaccgac tgggaattaa tcgtcagcat tgcctcgata acctgaaaat tctgcgtgaa
    19441 aatccgcaag tgcgcgaaaa agtggtggcg atattcgcgg aagccgaacc gtttacgcct
    19501 tcagataacg tggatgcaca gctttataac ggctttttca gtgacgcaga tcgtgcagca
    19561 atgaaaattg tgctggaaac cgagccgcgt aatttaccgg cactggatat cacttttgtc
    19621 gataaacgga ttgaaaagct gttgttcaat tatcgggcac gcaacttccc ggggacgctg
    19681 gattatgccg agcagcaacg ctggctggag caccgccgcc aggtcttcac gcccgagttt
    19741 ttacaaggtt atgcagatga attgcagatg ctggcacaac aatatgccga taacaaagag
    19801 aaagtggcgc tgttaaaagc gctttggcag tacgcggaag agatcgtcta atttgaagcc
    19861 tccgccgctg gtacggcgga ggtcaatgtt gagtcaggct ttttgaacgg tgatactcca
    19921 cgctgcatcg ccaatctgct gataatcggt gatggcatgc ccttcttctg ccgcccactg
    19981 tgggattgct tcagttgcct gggtgcaatc aaactcaatt accaactcat cgccgctggc
    20041 catttctgcc agtgctgctt ttgcttctat gagtggaaac ggacagacct gcgtcaccac
    20101 atccagcttt ttaatcgcca tctttacacc ttacttaatt tgccgcagca gttgccagtc
    20161 gcgccttacg ctgcggtcga acatacacaa gccaggacgc agtccagact ccgagaatca
    20221 taaatacaag gccaatccag ccctgccagg tcatcattgc agtcataacc agtccattac
    20281 cgataaagca accacctgca atactggcac cgaagcccat cagtacacca cctaacccgc
    20341 tacgtaatgt tgtttgtgca tcagctgcgc gaacgcggaa ctcacggctc gctttggctg
    20401 caataaatga ccccacgaag atccctaaca ccaggaaaac gccccagtta atgtatttca
    20461 cgtcacccgc gaccagaaat tgcagaatat tggccgttgg ggaagtgatt cctaacccaa
    20521 acatgcgtcc ggttgcttca ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta
    20581 cagcagtgac aaagggatgc cagcgttttt caaacagaat atgagcgatc ccagttcggc
    20641 gcggcggtaa ggtcgcgact ttgagttttg gcttcttcaa ctctttcatc accacccaga
    20701 gcgttatcac cagcaaaacg gcaaccaacg gccaaacaga caaattaaaa gtctcagcaa
    20761 tagagttatg ttcagtactg tagtgctgca aggtttgatt taaaccactg gcatgtggag
    20821 aacgcatcac cgcactcatc accatataag tgaaaagcgc gatccaactg ccgatcaatc
    20881 cctcacccgc gcgataccag gtcccggtgg cacatccacc cgccagaaca attcccagcc
    20941 cgaagatata cccacctata acagtaccaa gccacgggaa cgcctcggct tcgtaagtca
    21001 gtaggcccgc ctgaattaac gcaaaaaccc ccacgctttg taccgaaatt gcaatcagca
    21061 aggcgtaaaa catgcgatta tttttcgcga tatacatatc gcgaaaacca cctgtcaggc
    21121 aaaaacgccc acgctgcatg acaaatccca gcagagcacc acaaattagc ccgcttaata
    21181 tcattgaaaa cataaattaa ttaaccagat gaatgttaat gaggaaatta ttcatgactg
    21241 gtggaatgca gaccaataac caaattctct aaattagaac aaatggttat taatgaggat
    21301 ttcgataaaa aaaaataaaa aaaccggagc caaatattca ttcgactccg gttgattatt
    21361 agaaaagatg attatgcaac gtcttcgtac tgcggaactg ggttacggaa gctcttggtg
    21421 acgcaagcca ggtaaatcag accgatagca gcccaaatca gacccagaac cattgagctt
    21481 tcttccaggt taacccacag cgcaccaacg gtcagcgcac cacacatcgg caggaacaga
    21541 tactggaagt gatctttcag cgtcttatta cgcttctcac ggatccagaa ctgcgagatg
    21601 accgacaggt taacgaaggt gaacgccacc aacgcaccaa agttaatcag cgctgtagcc
    21661 attaccaggt cgaagttgat tgccagcagg gcaatcgcgc caaccaggat gatgttcatc
    21721 gccggagtac gccatttcgg gtgtacataa ccgaagaagc ttttcgggaa tacgccgtca
    21781 cgacccatta cgtacatcag acgcgctacg cctgcatgcg ccgccatacc ggacgccagt
    21841 acggtaatgg tggagaagat cagcgcgcca acctggaacg ctttacccgc aacgtacagc
    21901 atgatttcag gctgtgacgc atccggatct ttaaagcgag agatatccgg gaagtacagc
    21961 tgcaggaagt aagttgcaaa gatgaagatc atgccgccaa tcagcgcggt caggaaaatc
    22021 gcacgcggga tcacgcgctc tgcatctttg gtttcttccg acaggttgct gatgccgtca
    22081 aagccggtaa aggaaaagca caggattgtc gccccggtaa tcatcggaat aacatgcgca
    22141 tcgccagacc agaacggacg agtgctcgcc agcgtaccag cgccttcacc ttcaaatacg
    22201 ccataaacaa ccatgcccag aatcaccgcg atcagcacta cctgcaacac gacgattaca
    22261 gtgttgaagt tcgctacgga tttcagacta cgcaggttaa aggcggtcat aaaggccacc
    22321 agcgccacca caaacatcca cgatgggatg gaaggcacca gagcttcaaa atagattttc
    22381 gccagcagaa tgttgatcat cggcgcgaac agatagtcga gcagagaaga ccaacccacc
    22441 ataaagccga cagtcgggct aatggatttc tgggcgtaag tatatgcaga gccagcagaa
    22501 ggatagcggc gaaccagctt cccgtagctc agagccgtaa acaggatcgc aatcaatgcg
    22561 aacgcatagg ctgtcggcac atgaccatcc gtgaggcctg aaacgatacc aaaagtatca
    22621 aacagcgtca tcggctgcat ataggcaaga cccatcatta caaccggaac taacgtaagc
    22681 gttttacgca attccacgcg agaggtgttt ggagtaacgt tatgcgacat ggtcattctc
    22741 ctttacggtg ataaccgtcg cgtaagcaaa aaattgcccc atttttttgg attcctcagc
    22801 gacaacaact gtcgattttt agtaaatatc tatccggtac gaagcccggc ctcttggtat
    22861 gaatgattgg tttgaagcaa aaaataaccg acgctgatga aacgtcggtt tttagtcatt
    22921 ttttgacagc ggcgcattgt gcctaaatgg gggggaaatg acaagagaat gagaagcttg
    22981 tcataataat ttttctttaa atggctgatt ttccgtcatc agattttgca taaacaccgg
    23041 cgatcgcact atttgctaaa atttcatccc gccaccagcc gctggcaagc cctgcggtct
    23101 gttcgttcca gccaatccag acgggttcaa agagagtttg ggcgaccact ttcttctcga
    23161 ttaacgcgcc actatcgaga aaacgttgca ccagataacg gggtaaatag ccgcagccga
    23221 ggccgctaat ttgcagctcc agcttggttt taaaatcgaa aacggtaatg gcttcttgtt
    23281 cgtcgagaag ttgtgaggcc gtagaagcgc ctgcctgcgc agtgtccccc accacaatcg
    23341 cccgatatcg cttaatgata cgacggttta acggctcttc ttcctgggct aacggatgat
    23401 gaggcgcgac cgcaaagact tgttccagat cgcccaaccg cgagaaacca aactcactgg
    23461 aagatggtgg ctcatgcatc gcgccaacaa taatatccgc tcgcccctga gtcagggcat
    23521 cccaggaacc accgagtacg ccattgataa acttcaggcg cgtcacgcta tgatgttgat
    23581 aaaaagcttc aatcagtggc gcaagcagag aaaaaggaaa cgtgtcgtcc acgccaatca
    23641 ccagctcgtt ttcccagcct tcatgcagtt tgatcgcctg cttttccagt tctcgcacgg
    23701 tatgcagaac ttcccgcccc ttctctaata acatttttcc ggtacgggtg aatttagcgc
    23761 ggtgaccgct acgatcaagt aattgaatat taaggtcgct ttccagcttg tgaacggtgt
    23821 aactcagagc ggacggcgtc ttgtagagtt tagccgacgc cgccgcaaaa ctgccttctt
    23881 tttctaaggc atcgaggatc atcaacacat ccagcagttg tttcatacac gcccccttgc
    23941 agtgcatgcg gcgtcctgct ggcgtgatcg tttactccag cggcattacc agcggatcgg
    24001 gatactggta ttcaaatccc agttcattac aaatccgact gccatcaata atcttacctt
    24061 tgccgctgtc cagactattt ctgaactgcg gaggttccag ccccagtaaa cgggccatct
    24121 gcggatagaa aacattacgc gcagggtgag ctggcgcaca tatattatag atgtgtccgc
    24181 ctttaggtgc ctgtaacaac agagtgatag cgccaataac atcttctaaa tggactaaat
    24241 taacaccatg ttcaccatca ggcgcggttt ttccggcaaa gaagcgtccg ggatgacgtc
    24301 ccggtccgac cagccccgca agacgcagaa tatcgaccga agtaccgggt aaattgtgca
    24361 gccagtcttc gagttcttct aatactcgtc cactgttggt caccggatta cgcggggtgg
    24421 tttctttcac cgtgccttgc gcgtcgccat agacagacgt tgagctggta aaaataatgc
    24481 gaggaatacg atgggccagc gcgctatcca ctaactcttg taccgcttgt aaatagaact
    24541 catcgccggg gccgctacga cgtgccggaa gcgtaatgac cagcgcatcg gcatccatca
    24601 gggcatccag atcgtcagaa tcgcaaacta actcaggctc catgcgaagc agatagctat
    24661 caatgccact cattcgggcc gcttcgacac catcttgtgt ggttttactc ccggtgactt
    24721 gccagcctcg cgctgaaagt gacatcgcca gcggcatgcc taaccaccct aacccgacaa
    24781 ttgcgacctt tttcatccgt tatctcctga tacaccttag atctataagg ctacgctagc
    24841 gtatcaaaac tgacaattca ttctatgaat gaatctgttc aataatgata acgacatgct
    24901 gcaatgagca gtgaatcgtc ggtaaccgcg tataccagac ggtgctcctc tgtaatgcgt
    24961 cgggaccaga aacctgacaa attatgtttc aggggttctg gcttcccctt accttcaaat
    25021 ggcgttctgc gggtatcttt gataagttca ttgatctttt taacaattcg cttatctgtt
    25081 tcctgccagt acagataatc atcccatgat tcctcagacc agattagttt cactcaataa
    25141 tgtccttttc tgttcctttg cctgatttca ggctatcgat tgagtccatc aatctccggg
    25201 cgttagcggg ggagcgcagt agataagccg tctcttccag cgagttgtat tcttcgagtg
    25261 acatcagaac acaagcctct ccattctgac gagtaataag gatcggggca tgatcttcaa
    25321 cggctttcat cattgttgcc gacaaattct gacgcgcttc gctgtagcta attgtacgca
    25381 tgtcaatctc ctcttttgta cagtttattg tacaatgatg ggcgttaatt aactatttat
    25441 taattagttt gtagatcaag gtattgtcag tgagacgaaa atccaggctt cgctattttt
    25501 ggtgccgtca gcttagagga cagtcctctt agccccctcc tttccccgct cattcattaa
    25561 acaaatccat tgccataaaa tatataaaaa agcccttgct ttctaacgtg aaagtggttt
    25621 aggttaaaag acatcagttg aataaacatt cacagagact tttatgacac gcgttcaatt
    25681 taaacaccac catcatcacc atcatcctga ctagtctttc aggcgatgtg tgctggaaga
    25741 cattcagatc ttccagtggt gcatgaacgc atgagaaagc ccccggaaga tcaccttccg
    25801 ggggcttttt tattgcgcgg ttgataacgg ttcagacagg tttaaagagg aataacaaaa
    25861 tgacagacaa cactcgttta cgcatagcta tgcagaaatc cggccgttta agtgatgact
    25921 cacgcgaatt actggcgcgc tgtggcatta aaattaatct tcacacccag cgcctgatcg
    25981 cgatggcaga aaacatgccg attgatattc tgcgcgtgcg tgacgacgac attcccggtc
    26041 tggtaatgga tggcgtggta gaccttggga ttatcggcga aaacgtgctg gaagaagagc
    26101 tgcttaaccg ccgcgcccag ggtgaagatc cgcgctactt taccttgcgt cgtctggatt
    26161 tcggcggctg ccgcctttcg ctggcaacgc cggttgatga agcctgggac ggcccgctct
    26221 ccttaaacgg taaacgtatc gccacctctt atcctcacct gctcaagcgt tatctcgacc
    26281 agaaaggcat ctcttttaaa tcctgcttac tgaacggttc tgttgaagtc gccccgcgtg
    26341 ccggactggc ggatgcgatt tgcgatctgg tttccaccgg tgccacgctg gaagcaaacg
    26401 gcctgcgcga agtcgaagtt atctaccgct cgaaagcctg ccttatccag cgcgatggtg
    26461 aaatggaaga atccaaacag cagctgatcg acaaactgct gacccgtatt cagggtgtga
    26521 tccaggcgcg cgaatcaaaa tacatcatga tgcacgcacc gaccgaacgt ctggatgaag
    26581 tcatcgccct gctgccaggt gccgaacgcc caaccattct gccgctggcg ggtgaccaac
    26641 aacgcgtagc gatgcacatg gtcagtagcg aaaccctgtt ctgggaaacg atggaaaaac
    26701 tgaaagcgct gggtgccagt tcaattctgg tcctgccgat tgagaagatg atggagtgat
    26761 cgccatgagc tttaacacaa tcattgactg gaatagctgt actgcggagc aacaacgcca
    26821 gctgttaatg cgcccggcga tctccgcttc tgaaagcatt acccgcactg ttaacgatat
    26881 tctcgataac gtgaaagcac gcggtgatga cgccctgcgg gaatacagcg cgaagtttga
    26941 taaaaccacg gttaacgcgc tgaaggtgtc tgctgaggag attgccgccg ctagcgaacg
    27001 cctgagcgac gagctaaaac aggcgatggc ggtggcagta aaaaatattg aaaccttcca
    27061 cactgcgcaa aaactgccgc cggtagatgt agaaacgcag ccaggcgtgc gttgccagca
    27121 ggtcacgcgc ccggtcgctt cagttgggtt gtatattcct ggcggctccg ccccgctctt
    27181 ctcaacggta ttaatgctgg cgactccggc gcgtattgcg ggctgtaaaa aagtggtgct
    27241 gtgctcaccg ccgccgattg ccgatgagat cctttatgcg gcgcagctgt gcggtgtgaa
    27301 ggacgtgttt aacgtcggcg gcgcacaggc cattgccgcg ctggcgtttg gtacggaatc
    27361 tgtgccgaaa gtggacaaaa tcttcgggcc gggtaacgcc tttgtcaccg aagcgaaacg
    27421 tcaggtgagc cagcgtctgg acggtgcggc tatcgatatg cccgcaggcc cgtcggaagt
    27481 gttggtcatt gccgacagcg gcgcaacgcc ggatttcgtg gcttctgatt tgctttctca
    27541 ggctgaacac ggcccggact cacaggtgat tttactgacg cctgacgctg atatggcgca
    27601 tcaagttgcc gaagccgtcg aacgccagtt agcagaactg ccgcgtgccg aaaccgcccg
    27661 ccaggcactg aacgccagcc gcctgatcgt gactaaagat ttagcgcagt gcgtagagat
    27721 ctccaaccag tacggcccgg agcacctgat catccagacc cgcaacgccc gcgatctggt
    27781 cgatggcatc accagcgccg gttcggtatt tcttggtgac tggtcaccgg aatcggcagg
    27841 tgattacgcc tccggcacca accacgttct gccgacttac ggttacaccg ccacctgttc
    27901 cagcctcggg ctggcagatt tccagaagcg tatgaccgtg caggaactgt cgaaagaagg
    27961 tttctccgcg ctggcttcga ccattgaaac attggccgcc gccgagcgcc tgaccgccca
    28021 taaaaatgcc gttactttgc gtgttaacgc ccttaaggag caagcatgag caccgtgact
    28081 attaccgatt tagcgcgtga aaacgtccgc aacctgacgc cgtatcagtc ggcgcgtcgt
    28141 ctgggcggta atggcgacgt ctggctgaac gccaacgaat accccactgc cgtggagttt
    28201 cagcttactc agcaaacgct caaccgctac ccggaatgtc agccgaaagc ggtgattgaa
    28261 aattacgcgc aatatgcagg cgtaaaaccg gaacaggtgc tggtcagtcg tggcgcggac
    28321 gaaggtattg aactgctgat tcgcgctttt tgcgaatcgg gtaaagacgc catcctctac
    28381 tgcccgccaa cgtacggcat gtacagcgtc agcgccgaaa cgattggcgt cgagtgccgc
    28441 acagtgccga cgcaggacaa ctggcaactg gacttacagg gcatttccga caagctggac
    28501 ggcgtaaaag tggtctatgt ttgcagcccc aacaacccga cagggcaact gatcaatccg
    28561 caggattttc gcactctgct ggagctaacc cgcggaaaag ccattgtggt tgccgatgaa
    28621 gcctatatcg agttttgccc gcaggcatcg ctggctggct ggctgacgga atatccgcac
    28681 ctggctattt tgcgcacact gtcgaaagct tttgctctgg cgggccttcg ttgcggattt
    28741 acgctggcaa acgaagaagt catcaacctg ctgatgaaag tgatcgcccc ctacccgctc
    28801 tcgacgccgg ttgccgacat tgcggcccag gcgttaagcc cgcagggaat cgtcgctatg
    28861 cgcgaacgag tgacgcaaat tattgcagaa cgcgaatacc tgattgccgc actgaaagag
    28921 atcccctgcg tagagcaggt tttcgactcc gcaaccaact acattctggc gcgctttaaa
    28981 gcctccagcg cagtgtttaa atctttgtgg gatcagggca ttatcttacg tgatcagaat
    29041 aaacaaccct ctttaagcgg ctgcctgcga attaccgtcg gaacccgtga agaaagccag
    29101 cgcgtcattg acgccttacg tgcggagcaa gtttgatgag tcagaagtat ctttttatcg
    29161 atcgcgatgg aaccctgatt agcgaaccgc cgagtgattt tcaggtggac cgttttgaca
    29221 aactcgcctt tgaaccaggc gtgatcccgg aactgctgaa gctgcaaaaa gcgggctaca
    29281 aactggtaat gatcaccaat caggatggtc tgggaacaca aagtttcccg caggcggatt
    29341 tcgatggccc gcacaacctg atgatgcaga tcttcacctc gcaaggcgta cagtttgatg
    29401 aagtgctgat ttgtccgcac ctgcccgccg atgagtgcga ctgccgtaag ccgaaagtaa
    29461 aactggtgga acgttatctg gctgagcaag cgatggatcg cgctaacagt tatgtgattg
    29521 gcgatcgcgc gaccgacatt caactggcgg aaaacatggg cattaatggt ttacgctacg
    29581 accgcgaaat cctgaactgg ccaatgattg gcgagcagct cactaaacga gaccgttacg
    29641 ctcacgtagt gcgcaacacc aaagagacac agattgacgt tcaggtgtgg ctggatcgcg
    29701 aaggtggcag caagattaac accggtgttg gcttctttga tcacatgctg gatcagatcg
    29761 ccacccacgg tggtttccgt atggaaatca acgtcaaagg cgacctctat atcgacgatc
    29821 accacaccgt cgaagatacc ggcctggcgc tgggcgaagc gttaaaaatg gctcttggcg
    29881 acaaacgcgg tatttgccgc tttggttttg tgctaccgat ggacgaatgc cttgcccgct
    29941 gcgcgctgga tatctctggt cgcccgcacc tggaatacaa agccgagttt acctaccagc
    30001 gcgtgggcga tctcagcacc gagatgatcg agcacttctt ccgttcgctc tcatacacca
    30061 tgggcgtgac gctacacctg aaaaccaaag gtaaaaacga tcaccaccgt gtagagagcc
    30121 tgttcaaagc ctttggtcgc accctgcgcc aagccatccg cgtggaaggc gacaccctgc
    30181 cctcgtcgaa aggagtgctg taatgaacgt ggtgatcctt gataccggct gcgccaatct
    30241 gaactcggtg aagtcagcca ttgcgcgcca cggttatgaa ccaaaagtca gccgtgaccc
    30301 ggacgtggtg ttgctggccg ataaactatt tttacccggc gttggcactg cgcaagcagc
    30361 gatggatcag gtgcgtgagc gcgagctgtt tgatctcatc aaagcctgta cccaaccggt
    30421 actgggcatc tgcttaggga tgcaactgct gggtcggcgc agcgaagaga gcaacggcgt
    30481 cgacttgctg ggcatcattg acgaagacgt gccgaaaatg accgactttg gtctgccgct
    30541 gccgcatatg ggctggaatc gcgtttaccc gcaggcaggc aaccgcctgt ttcaggggat
    30601 tgaagacggc gcgtactttt actttgttca cagttacgca atgccggtca atccgtggac
    30661 cattgcccaa tgtaattacg gcgaaccgtt caccgcggcg gtacaaaaag ataacttcta
    30721 cggcgtgcag ttccacccgg agcgttctgg tgccgctggc gctaagttgc tgaaaaactt
    30781 cctggagatg tgatgattat tccggcatta gatttaatcg acggcaccgt ggtgcgtctc
    30841 catcagggcg attacggcaa acaacgcgat tacggtaacg acccgctgcc gcgcttacag
    30901 gattacgccg cgcagggtgc cgaagtgctg catctggtgg atctgaccgg ggcaaaagat
    30961 ccggcaaaac gtcaaatccc gctgattaaa accctggtcg cgggcgttaa cgttccggtg
    31021 caggttggcg gcggtgtgcg tagcgaagaa gacgtggcgg cgttactgga agcgggcgtt
    31081 gcgcgcgtgg tggttggctc caccgcggtg aaatcaccag aaagggtgaa aggctggttt
    31141 gaacgtttcg gtgccgatgc cttagtgctg gcgctggatg tccgtattga cgagcaaggc
    31201 aacaagcagg tggcggtcag cggctggcaa gagaactcgg gcgtttcact ggaacaactg
    31261 gtggaaacct atctgcctgt cggcctgaaa catgtgctgt gtaccgatat ctcgcgcgac
    31321 ggcacgctgg caggctctaa cgtctcttta tatgaagaag tgtgcgccag atacccgcag
    31381 gtggcatttc agtcctccgg cggtattggc gacattaacg atgttgctgc cctgcgaggc
    31441 actggcgtgc gcggcgtaat agttggtcgt gcattactgg aaggtaaatt caccgtgaag
    31501 gaggccatcg catgctggca aaacgcataa tcccatgtct cgacgttcgt gatggtcagg
    31561 tggtgaaagg cgtacagttt cgcaaccatg aaattattgg cgatattgta ccgctggcaa
    31621 aacgctacgc cgaagaaggt gcagacgaac tggtgttcta cgatatcacc gcttccagcg
    31681 atggccgtgt ggtagataaa agctgggtat ctcgcgtggc ggaagtgatc gacattccgt
    31741 tctgcgtggc gggtgggatt aagtctctgg aagatgccgc gaaaattctt tcctttggcg
    31801 cggataaaat ttccatcaac tcccctgcgc tggcagaccc aaccttaatt acccgcctgg
    31861 cggatcgctt tggcgtgcaa tgcattgtgg tcggcattga tacctggtac gacggcgaaa
    31921 ccggtaaata tcatgtgaat caatataccg gcgatgaaag ccgcacccgc gtcactcagt
    31981 gggaaacact cgactgggta caggaagtgc aaaaacgtgg agccggagaa atcgtcctca
    32041 acatgatgaa tcaggacggc gtgcgtaacg gttacgatct cgaacaactg aaaaaagtgc
    32101 gtgaagtttg ccacgtcccg ctaattgcct ccggtggcgc gggcaccatg gaacacttcc
    32161 tcgaagcctt ccgcgatgcc gacgttgacg gcgcgctggc tgcttccgta ttccacaaac
    32221 aaataatcaa tattggtgaa ttaaaagcgt acctggcaac acagggcgtg gagatcagga
    32281 tatgttaaca gaacaacaac gccgcgaact ggactgggaa aaaaccgacg gactaatgcc
    32341 ggtaattgtg caacacgcgg tatccggcga agtgttaatg ctgggctata tgaacccgga
    32401 agccttagac aaaaccatcg aaagcggcaa agtcaccttc ttctcgcgca ctaaacagcg
    32461 actgtggacc aaaggcgaaa cgtcgggcaa tttcctcaac gtagtaaata ttgcgccgga
    32521 ctgcgacaac gacacgctac tggtactggc gaatcctatc ggaccaacct gccacaaagg
    32581 caccagcagc tgcttcggcg acaccgctca ccaatggctg ttcctgtatc aactggaaca
    32641 actgctcgcc gaacgcaaat ctgccgaccc ggaaacctcc tataccgcca aactgtatgc
    32701 cagcggcacc aaacgcattg cgcagaaagt gggcgaagaa ggcgtagaaa ccgcactggc
    32761 agcgacggta catgaccgct ttgagctgac caacgaggca tcggacttga tgtatcacct
    32821 gctggtgctg ttgcaggatc aggatctgga tttaacgacg gtaattgaga acctgcgtaa
    32881 acggcatcag tgagttgcag ggtaagcgga tgcgatgttg ttgtcgcatc cggcaaaaaa
    32941 aaagggcaag gtgtcaccac cctgcccgtt ttctttaaag ctgcaaagat tacttcgcgt
    33001 tataattacg cagagcgtta cgccccagca caatccccgc gccaaccatg ccgcccagca
    33061 gcaccgccag aatcaaggta attgcctttt tcgggctatc gcgacgaata ggtaacgtcg
    33121 gtttcatcac ataacggtaa gcatgaatat caagatcatc aacctttaag ttttcaatat
    33181 ccaacaggtt ttgacgtgtc tgatagtagt ttggtgagaa caccaacgga cgggtcgcct
    33241 catgcttaat catcgactcc agcgcttcgc tccccaaaag gaacaacgta tcttgtgtca
    33301 catcttcagt ctgctgaacc tgtggctttg ccacctgcgc ctgattcgca tactgcaacg
    33361 cttcctgaat ctgacggata cgcagatctt tctgctcctg cgcgactact tcctgcgttc
    33421 ttaaagagtc ctgcaagttt ttccgtccca gagcaatgtt gtctttgaga tccttttcta
    33481 gctcttgatt cactttatca tcaacttgct gaatgtattg ggccaacttc atttgtgctc
    33541 cctctgcagt ttgcccaaca taagaaacag tcaatggtaa ttgctggttc ttaacagaag
    33601 gttcgatggt aagtttttct ggttcttcct gattatccag cgtttctgct aatgcagaga
    33661 aggcagaact gaagcgacca attaacgtct cctgtaaatc cgatactttc ggtgcagcct
    33721 gaccatagat aacattcatg gcattgttat agccagcaat ttgccccacg tcgggctgag
    33781 tgataattgc tgttgacgtc catttctcct tcgctactgc caaatatcca atagccaggg
    33841 caatagccac aatgacggaa atgatgattg tcatcttgcc acgccacaac tgcgctagta
    33901 aatcaatcaa atcaatctgt tccgggtcat ggttttgccc agaaacatta ttattttcta
    33961 ctctcatcat taccctaact gacggaaatt tccaagtccg catagtttag cgataatcct
    34021 agtgaatgct ataggataag tgataaaaat aatgaattat tggggaggta acaaaagaca
    34081 atcagggcgt aaatagccct gataacaaga tgttagtcgc tgccaaagag atcgcgggtg
    34141 tataccttat ctgccacatc tttaagctct tctgccattc ggttagagat aatgacgtcg
    34201 gcttgttgtt tgaagatggc gagatcacgt tccagccgag agttgaagaa tgagtcttct
    34261 ttcatcactg gctcgtagat gatcacttca acgcctttcg ccttgatacg cttcataatc
    34321 ccctgaatag aagacgcacg gaagttatct gaaccgctct tcataatcag acgataaata
    34381 cccaccactt gcggtttgcg tgacaaaatg gcatcggcaa taaaatcctt acgcgtgcgg
    34441 tttgcatcga caattgccga gatcaggtta ttcggcacag actgatagtt cgccagtaac
    34501 tgcttggtat ctttcggcaa gcaataacca ccataaccaa acgacgggtt gttgtaatgg
    34561 ttgccaatac gcggatcgag acaaacgcct tcgataatct ggcgagtatt cagacctaaa
    34621 ctttctgcgt agctatccag ttcgttaaag tatgccacgc gcatcgctag gtaggtgttt
    34681 gcaaaaagtt taatcgcttc tgcttcggtc gaatcggtga acagggtcgg gatattttgc
    34741 ttaatcgcgc cttcctgtaa caacgcagca aaacgttctg cgcgttctga acgctcaccg
    34801 atgacaatac gtgaaggatg gagattatcg taaagggctt taccttcacg gagaaattcc
    34861 ggagagaaaa taatattttc agtacgatat ttcttatgca tcgcttcggt aaaaccaacg
    34921 ggaaccgttg atttgatgac cataaccgca taaggattta tctcaactac gtctttaatt
    34981 actgattcta cactggatgt attgaaataa ttagttttag gatcatagtc ggttggagtg
    35041 gcgatgatga cataatcagc atcccggtaa gcttcatttt tatctaatgt ggcattaaag
    35101 tgtattttat ctgattgcaa aaactgctga atttccttat caacaatagg agatatccga
    35161 tcattcagca tagcaacgcg tgacggtaaa atatctaatg ccacaacctc atgattttgt
    35221 gcgattagaa gcccgtttga caagcctaca tagccagtac cggaaatggt gattttcatt
    35281 tcatgctctc agaattaact taactgtgaa tcatgatgtt tttagcatcc tgataagagc
    35341 taaatgtttt aacgctacgt tattagctgg tcaacgctac gaaaaacaat tcagattttc
    35401 tttataagaa tagctgatat ttattaagtt aatattaagc agtgaaattt agtctataag
    35461 ataaatggca aaaaaaaagc ccggtgcaat atacgccggg cctcaatttt actgttggtt
    35521 aaatcagatt aatccagcca ttcagtatgg aacacacctt ctttatcaat gcgcttataa
    35581 gtatgtgcac cgaaatagtc acgctgtgcc tggatcaggt tcgcaggcag aacagcggca
    35641 cggtagctat cgtaataggc aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt
    35701 actgcataag caacgacatc acgcagcgcc tgctggtagt catcggcaat ttgcttgaag
    35761 tacggagcca gcagcaggtt agcgatctgc ggattttcgg catatgcatc ggtgattttc
    35821 tgcaggaact gcgcacggat gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag
    35881 ttcagatccc agttgtattc ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa
    35941 gaaacgattt tgcccagata cagcgcacgg cgaacttttt cgatgaactc agccttgtcg
    36001 cctgctgact gagcttgcgg accagagaga actttagatg cggcaacgcg ctgatctttc
    36061 agagaagaga tataacgtgc aaacacagac tcggtaatca gcgacagcgg ttctccgaga
    36121 tccagcgcgc tctggctggt ccatttaccg gtacctttgt tagccgcttc atccaggatc
    36181 acatcaacca ggtagttacc gtcttcatct tttttagtga agatatcttt ggtgatgtcg
    36241 atcaggtagc tgctcagttc accgttattc cactcggtaa aggtctgtgc cagttcttcg
    36301 ttagagagat tcaggccgcc tttcaggaga gaataggctt cagcaatcag ttgcatatca
    36361 ccgtattcaa taccgttgtg aaccatcttc acataatggc ctgcgccatc agcaccgata
    36421 taggtaacgc acggttcgcc atcttcagca acggcggcga ttttggtcag gatcggcgca
    36481 accagttcat aggcttcttt ctggccacca ggcatgatgg acggcccttt cagcgccccc
    36541 tcttctccgc cggaaacacc ggtaccgatg aagttaaagc cttcggcaga aagctcacgg
    36601 ttacgacgaa tggtgtcctg gaagaaggtg ttaccaccat cgatgatgat gtcgccttta
    36661 tcgagatatg gtttgagcga atcaatagca gcatccgtgc ctgcacctgc tttcaccatt
    36721 aacaggatgc gacgaggcgt ttccagagat tcaacaaact ctttcaccgt atagtaagga
    36781 accagtttct tgcctggatt ttcggcaatc acttcttccg tcttttcacg ggaacggttg
    36841 aaaatagaga cggtataacc acggctttcg atgttgagcg caaggttgcg ccccatcact
    36901 gccataccga ctacgccgat ctgttgcttt gacattgttt actcctgtca gggggtggtc
    36961 accgccgagt gtgcggctta aatgtggtat agatgttaac tcactcatag agatggtgaa
    37021 tagcattacc tttgctatac cacactgaat atctcacctt ttccgtcttt taaaacttat
    37081 ttatatgaaa ttactgaatt acttgttcag taactcaaga ataagttttg tcttttcttc
    37141 catcagcggc acatcaccgc gagattccac attcaaccgc accaccggtt cggtgttgga
    37201 ggtgcgcagg ttaaagcgcc agtcggcaaa ggtcatgctg atgccatcgg tgcgatccac
    37261 cgccagcgcc tcgcggctaa aatgctgttc cacgcggtta atcgcctcaa agggttgcgc
    37321 cagtttgctg ttgatctcac cgcttgccgg aaacgctgcc atccggtcgc gcaccagttc
    37381 gcccagcgtt tttcctttca ggcacaccag ttcggcgacc agcagccacg ggatcatgcc
    37441 gctgtcgcag taagcgaaat cacggaagta atggtgggcg ctcatttcac caccgtagat
    37501 ggcgtcttcc ttgcgcatcc gttctttaat aaaggcgtgt ccggttttcg acatcaccgg
    37561 cgtgccgcct gcggcagtca ccacatcaac ggtgttccag gagagacgcg gatcgtggat
    37621 gatcttcgcg ccgggatttt tttcgaggaa tgcttctgcc agcaggccga caatgtagta
    37681 gccctcaata aactgccctt tttcgtcaaa caggaagcag cggtcaaaat cgccatcaaa
    37741 agcaatgccc atatccgcac cgtgtttgat gaccgcattg cgggtgtcgt cgcggcattc
    37801 cggcagtagc gggttaggaa taccgttggg gaaattgccg tccggcgtgt tgtgcacttt
    37861 gattaattcc acgggtgcgc caagggcttt aaagcgggct tcgatggcgt ccaccaccgg
    37921 acccgctgcg ccattcccgg agttaatcac cagcttgagc ggcgtgaggt ttttgacatt
    37981 gatataaccg aacaggtgat caacgtaagc gtcacgcaga ttgatttgct gatagcgacc
    38041 gcgtttggtt tcatcaacgg gaggaaagtc gttggcttct gccagacgct ggacatcgcg
    38101 cagtccggta tcaccgctga tcgggcgagc cccttcgcgc accagtttca tgccgttgta
    38161 atccatcggg ttatggctgg cggtaacttc aatgccgcca tccacgccga gatggaacgt
    38221 ggcgaaatag atctcttcgg tgccggacat gccaatatcc agcacatcga cgcccgcatc
    38281 ctgtaaacct ctcgccagcg ccagttttaa ggtttcgctg gtgaggcgga catcaccgcc
    38341 taacacaatg gttttcggtt tgagaaattc gccataggcg cgaccaatgc gccaggcgat
    38401 atcttcattc agttcttcgc ctaatttccc gcgaatatca taggctttaa agcaggttaa
    38461 ttttttcatg tcgttaccct ttttcaggca atagttggcc ctgaccgaaa cggccaattt
    38521 tgtttttgtc gttatttaca tctcggtttc gcattcattg cctgatgcga cgtttacacc
    38581 cgtccgtagc gatccgcgaa acgcaccaca tcatcctctt cgagataaga gccggagcgc
    38641 acttcaatta aatcgagcgg aattttcccc gggttttcca ggcaatgcat cgcccccagc
    38701 ggaatataaa tggactcgtt ttcaccaagc agtttgatat caccatcaat ggtgactttt
    38761 gccgttcccg cgacaaccac ccagtgttcc gcgcggtgat ggtgcatctg taccgacaag
    38821 ccctcgcccg gtttcacggt gatgcgtttc acctggtagc ggtcgcccgc gtcgatagag
    38881 tcatatttgc cccacgggcg atagacttcg cgatgcaccc gatgctcatg gcgaccatcg
    38941 gctttgatct gctcgaccac ttttttcaca tcctgcaccg cgttacggtc ggcaatcagc
    39001 accgcatctt tggtctgcac cactaccaga tctttcaccc cgacggtggt tacgaggcca
    39061 gattcggcgt acacatagct gttttcagtt ttgtgattaa tcacatcgcc gtggcaaacg
    39121 ttgccctcgg cggtgtgggc gctaatttcc cataatgagg accaggagcc gacatcgctc
    39181 cagcccgcat ccatcggcac cacaacggca tctgccgtac gttccatgac cgcgtaatcc
    39241 accgactctt ccgggcaggc gagaaacgct tcttcatcca cacgaataaa atcgagatcc
    39301 ggatcgacgg cgctcatcgc tttttcacag gcatcgagaa tatccggacg atattttttc
    39361 agttcttcga gatagcgtcc ggcgcggaac aggaacatgc cgctgttcca gtaatattcg
    39421 ccgcttgcca cataggcctg ggcggtttcc agattcggtt tttcgacaaa ctgcgccact
    39481 tcaaaggcca ccgtatcctg ctcacccgca gacacttcac cgcgacgaat atagccataa
    39541 ccggtttctg gtagatccgg cacaatgccg aaggtcacca gcttgcccgc ttcggcatat
    39601 ggcatggcat tacgcacggc ggcacggaac gcgtcttcat cggcaatcac atgatccgcc
    39661 gccaatacca gcattaacgg gtcgctctcc gggctatgac gttttgccgc cagcgccgcc
    39721 agcgcaatgg caggtgccgt gtttcgccct gccggttcga gaataatgtt ctcggtaagt
    39781 ttgttcagtt gacgcagctg ttccgcgaca ataaagcggt gctgctcatt gcaaatcacc
    39841 accgggcttt cgcactccac gccgttcagg cggcagatgg tggtttgcag catggtgaga
    39901 tcgcctttca ggcataaaaa ctgcttgggg taaagtacgc gggaaagcgg ccataagcgg
    39961 ctaccggagc cacctgccat cacaactgga tagagtttcg actgcgccat aattatcccc
    40021 gaatatcatt tataaattga cgtaacacgt tctctttatc gagtgtgcgt tcggcatatt
    40081 cacgtgccac cgtgttgtgt ttgggcagca ggagcgcctg acggatcccc gccaccagcg
    40141 cctcgaccga ttccggttct acgcaaaccg caatgcccgg aaaggtttcg caaagctgcc
    40201 ccagttctgt gtgggcctct gcggtaatca ccgcgttacc gcctaccgcc agaatattgg
    40261 tcagtttcga cggcaatacg gcatctgccg cgccgcgttt ttgcaccacc agatggcaat
    40321 cgcccatctt cagcagtgcg ggtaaagcgt catacgattg cagcgggaaa aattgcatgt
    40381 tgcgcagtcc acgctgctgc gccatttttt ccagccgcgc tttgccgccg ccctgcccga
    40441 caatggcaaa aatcagcggt tcatcgcgca gacgatcggc agcttcaata acgttttcca
    40501 gcccctgctt ttcaccaata ttgccggagt aaagaatgat ttttttgtta tccggcaggt
    40561 caagctggtt acgaagggcg tcaacatcgg catctgcaac atgctgaaaa cgggcaattt
    40621 ccgaccagtt ggggaagaag atgacgtttt ccgccgccac gcctttttcg atggctttat
    40681 tcatcatcga acgcgaaatc gtggagacgt tatcgacgtt atgcagtccg ctacgttcga
    40741 acgccgttgc cagctgtgcc actttgctgc ctttgccttt tccggcaagg cccagcccca
    40801 gcatggcgtc cacttcgtaa tcctgaatat gcagcacggt acgcgcacca gaaagtttcg
    40861 ccagcaggcg cattcccggc gtgcaaaaca gcgttggcac cacgccgata atgcgatccg
    40921 gcttccagcg acgttgcgcc atcagaggaa agaaactgct gacggcaaaa ctgccgagat
    40981 gtaacaggcg tttcagggtg ctcggctgtt ttggcacgta cagcgggcag cgccacaccg
    41041 tggcggcccc ctcttctcgt ttgtaccgcc aggcggaata gttctcgccc acctgccact
    41101 gcgggtagta aggcggtgcg gtaataaccc gcacctcatg accttgtgcc gccagccatt
    41161 ccaccatctc gccggtgtat ttgccgatac cggttaactc cggcgagtag ttaatgccgt
    41221 agaccagtat tttcataatc cgggtactcc ggcacgcttc tcagcgagaa aataggcgcg
    41281 gctgttagcg tgaacatcgt tactggcgag cagcgcgtcc ggcgtcagcc agcggtaatc
    41341 gtcatgctgc tcatccggca gtatcagctc ttcttcagcg actctgaagc gaaaaccgag
    41401 caccacatag tgagtggtga aatccgtgcc ggagaagtta tcgtcataaa agtgctgcca
    41461 gacaccgtaa aactggcctg ctgttatcgg cagacgcagc cccagttccg ccatcgtcag
    41521 ccgctcgaat gcggcttcca gcgtttcgtc tttctgcacg cgccctcccg gcacaaacca
    41581 gtaaccctgc gccgggcggt tggttctttt gccaagcaga aactcaccgc gactgttctc
    41641 gacaataaag tcgagagaga caagcggggt ggagcgcact accgtggcaa agtcttcctg
    41701 acgtaaaaac atcattaccc ccgaaagcgg tcttgattct caaggaacca ctggtaagtg
    41761 ctggcaagcc ccgcttccag tgagatttcg tgataccagc caagctgatg caggcgcgtc
    41821 acatccagca gtttgcgcgg cgtaccatcc ggtttgctgg catcaaaaac cacccgacct
    41881 ttgtaaccca ccactttggc gatggtttgc gccagctcgc ggatagtgca gtcaacgccc
    41941 gtgccgacgt taatgtgcga cagcatcggc tgggtgttct ccagccagac ttcgtgcgcc
    42001 agctccatga catgaatgct cgccgccgcc atatcatcga cgtgcaggaa ttcacgcatc
    42061 ggtgtaccgc tgccccatac caccacgtcc ggtgcattct gtgccgtcgc ctcgtggaag
    42121 cgacgcagca atgcagggat cacatgcgaa ttactcgggt ggaagttgtc atgcgggcca
    42181 tacaggttgg tcggcatgac tgagcggtaa tcgcgtccgt actggcggtt gtatgattcg
    42241 cacagtttga tcccggcgat tttggcaatg gcgtaaggct cgttggtcgg ctccagcgtg
    42301 ccctgcaata actcgctttc tgccatcggc tgttttgcca gtttcgggta gatacaggac
    42361 gatccaagaa acagcagttt gttcacgtcg ttctgatgcg cggcgtgaat gatgttgctc
    42421 tcaatcatca tgttctggta gatgaaatcc gccggatagg tgttgttggc aacaatgccg
    42481 cccactttcg ccgccgccag atagacctgg tcaatgcgtt cgctggcaaa gaaatcatgc
    42541 accgcgcggc tgtccagcag gttcagctcg tcgcgggtgc gtaataccag ttccacatca
    42601 ccgcgctgtt cgagctgccg cctgatggca gaaccgacca tcccacgatg gccagcgata
    42661 aaaatgcgtt gtttactcat gcttatgact ccagcgcgat cgccacgtcg tagccgtgag
    42721 atttcagcag agagtgtttt ttcgccgctt cgaggtcatt agccaccatt tcagacacca
    42781 tctctctgag ggtgatttcc ggtttccagc ccagtttttc gtgcgctttg gtcgggtcgc
    42841 cgagcagcgt ttcaacttca gccggacgga agtaacgcgg atcaacggcg ataatcacat
    42901 cacccggttt aacgcccggc gcgtcatgcc cggtgacgga aaccacaatg cccttctctt
    42961 caacacccgt gccttcaaag cgcagtttga tgcccagctg tgccgctgcc atttccacga
    43021 actgacgcac ggagtactga acgccggtcg cgataacgaa atcttccggc tgttcctgtt
    43081 gcagcatcat ccactgcatt tttacgtagt ctttggcgtg gccccagtca cgcagggaat
    43141 ccatattgcc gaggtacagg cacgactcca gcccctgggc gatgttggcg attgcgcggg
    43201 tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga ttcatggttg aagagaattc
    43261 cgttacaagc gtacatgccg taggattcgc ggtagttaac ggtgatccag taggcgtaca
    43321 gtttggcgac cgcatacgga gatcgcgggt agaacggcgt ggtttctttc tgcggaattt
    43381 cctgcaccag accatacagt tcagaggtgg aagcctgata gaaacgagtt ttcttttcca
    43441 gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc catcgcgtcg acgtcagcgg
    43501 tatattctgg tgactcaaaa gagaccgcaa cgtggctcat tgcgcccagg ttgtacactt
    43561 catccggctg tacttcacgc aaaatgcgcg tcaggttaga ggtatcactc aggtcgccat
    43621 aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg ataaatgtga tccacgcgct
    43681 cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac ctcgtaacct ttttccagca
    43741 gaaactctgc caggtaagaa ccgtcttgtc cggttacacc ggtgatgaga gcgacttttg
    43801 acatgtctta ttcctctgta tttttgaatt tattcagttt caacgcgttc gcgtatcacc
    43861 actgcgggat tcccccggca aaccacattt gccggaagcg atttaaaaac actgcttcgt
    43921 gcacccacga cggtgccgtc gccgattgtg acgcctgggg caacaaagac atcggttgcc
    43981 agccagcatt tctcgccaat cacaataggc gtggcgttaa tggtgaaatg ttgacttgca
    44041 tggtcgtggc taccggtgca taaataactt ttttgcgata tcaccgaatg tgcgccaatg
    44101 gttatttcac cgagggtata taaattgacg tcatcgccga cccacgcgta atcacctaag
    44161 gttaatttcc acggataggt aatttttact gacggacgaa taactacgtt ttttcctatt
    44221 tttgctccga ataaacgtaa taaaaaagcc cgccagcgat acaatacttg tggcgaccag
    44281 gcaaatattg ttgcctgtac tgcccaccat aattgcactt taatagcgtt gccgccccgg
    44341 aaccctttcg gcaccgagaa accgcttaaa tcttgcattg gttttcctta tattcagact
    44401 ttgttatata aggctttcgt ctttgaggta gtacgttggc gtaaatgcca ggataattca
    44461 gcccaaaagc caggcacatg taatatttgt cgttggactt ttttcgcgtc agcacacaat
    44521 tccatattat tggtggtaga taccccaccc atggaaaatt cagacaccag gccattgagt
    44581 tttttaaatg cataaccggc tttatacatt ttggctgcca gcgcgtagtc ggaagaaact
    44641 ttatattcca ggtcataacg ccattttttc aagccggata cagggaaaaa tatcgcctga
    44701 tgactggcgg gcaggctgtg ataaatatac cagcccggtt tggcgctacg tttaatttta
    44761 tgcccgtcgc caaaatccag cagcgcatcg ccggtgatca tcatgttatc tttttgcatt
    44821 tttaacttac ggacaaaatt tgcggcatcc tgatgaaaaa tatcgcccga attgagaaac
    44881 aacgcgaact tgccttgtgc catcgtaatg cctttgttca tggcgtcgta gataccgtta
    44941 tctggctcgc tgacaaagcg taggttatag ataccattga gattttccag atactcacgg
    45001 gtgccgtcat tggaaccgcc atcgacaaca atccattcga agctgatatc ttccgcctgc
    45061 gccagatgcg ccagcgaggc atgtgttttg actatccctt cgaggttacg aaacgcgaca
    45121 gtgattatgc taagcaacat gttcttattc ttacctcgta atatttaacg cttttcgcaa
    45181 aataaacgga cagacgatta aaaatgcata ttccgggcta aatatcgaac cggtaaaaaa
    45241 cagcgatacc ggagtaaaaa gatataattg cacgcgaaaa ttgcggttat caccaaaagc
    45301 gttgatcatc atttttatca ctttccccat gtaccacagc gataaaaaca ctgcgaacca
    45361 ggaaaaataa ataatcagca gatacaaacc attgtctatg gtttttccga catccgcacc
    45421 gttaaatatt ccgaatgatg cgacgtattc ataaagtgag ccaaatctga ctacaccgtc
    45481 aatatgggtc aaagaataac cgaccatcac cagcggaccg acaatacgat aatacgatga
    45541 cgatccttcc gtacctaaat cgcccagacg ggtggaaata taaggaaacg cgattaccac
    45601 accaacccgg aatacagcca gagaaatcaa tgccaacggt aactttttct taatcgcctc
    45661 tttattcaga tattgaaatg cccactccag caaataaaac aggataaaag tcataacccc
    45721 tgaaaacgat ccggataata ttatccctgc gagaatcata gcatcggttt taggcgtttt
    45781 gataccaaac tgtttgatgc tgagccaaat tgagattaat gccagagcga aaaatgccgg
    45841 ttcgaaataa agtgctgtcg tgcgcttgcc gccgaattta atgaaattca gtacatagct
    45901 gttgctgtaa atcagatatt tcgaaatcga ctccataata ctgctgccac cggtgaggat
    45961 aatttgcgcc atctccaccg ccgccagcgc caccaccagc ccgatcacca gataaaagaa
    46021 acgtaatatc ttgcgatggt tgtgtggcga aatcgtttta aagcgaatac tccacaccat
    46081 gccaataatg atcacaatat agacaaacag catggttgaa gtgacgtatt tactggcatc
    46141 cagcgactga ccaaacagat agttaaacgc cgtgagtccc gcaccaatcc ctaaggcaat
    46201 catcaatttt ttaacgctga tgcgttctaa aaacagcagt aacaagacag gtaaaaaagt
    46261 gacgatggtg atggggaaac tttcgccaag ctgggcgatt ttgacgttaa ccagcaggta
    46321 gatcagcggc agcagcaggt agctacagat tctgatagaa gttgacatac tcctccagca
    46381 tctgttgtcc actgtaggtg gcgcggctgc gttggctgaa ctcagccagc gtggtaccaa
    46441 atatcgcctg cgcgatttcc ggtttgctta actgcaccag ttgcagcacg tcttcttcgt
    46501 cgacggtttt accgccggat ttttgcaaca cttcccgtgc cgcgtcgcta tgggtggcaa
    46561 tcaccggcac gccaattgat aacgcctcac acaaaatcag cgggtagtta tcgacgcgag
    46621 aactgaatac cagcgcatcc atctgattga gcgcgctcat caacttgcgc ttgtcagttt
    46681 caaagccgtg attaaccacg ttgccagcgg tgaacggcga gaacttacca aaggtatgca
    46741 gttcgatttt gtcgcccagc gccatcatct cacgcaccag ttgctggtta gtcttgccgt
    46801 cgtaacgcaa gtcatgcgcc accaccgcga ttttcggctt gccctgggtt tcgcgcaccg
    46861 gaggcaagtc cgccagaatc gcttcggttg ccatatcaat accattattg ataatccggc
    46921 aacgccctgg accgtacagg ctattgaaag cgtcagccac atgctggctg ggggaaataa
    46981 actgacagcc cagcgccagc atctcacgga ataactggcg tttgcccgcc accagctggt
    47041 gtgcgcgatc aatcttcacc ggcggatagt tatttaaggt cgggcatttc tggcagcccg
    47101 ttttccagcc ttcgcaaccg tcggtaaagg cgcagcgtcc ggtaacactc cagtggtcgt
    47161 gcagcgtcca gaccagagtg acgtccggtt tatggttttt cactttttcg caaaagcgca
    47221 ccacgctctt aagatttagc cagtagctgt gcagcacatg aaaatgcagg accaccggac
    47281 ccggtgtgcg agtaatggtg cgatataact cattgaaatt gccaaacaga tcgcgattaa
    47341 acagacgaaa cagggcaata ttcgccatcg cggtcatccg cggcgtatgt ttgatgacct
    47401 gcggatagtt ctgatggctg acgctctctt tgccgccttt gccataaccg tagacaaaat
    47461 gtgacgccag cccctgttgc agcgcacgct ggtggagatc taacgccaca cctgctgccc
    47521 cgccttccgc cagtcgcaca ttaaattgca gaatattcat ttaattacct tcactcgcgc
    47581 tttttctccc accaccagcg cgttgtccgg gacagagtcg agcaccacgc tgcccgcacc
    47641 cacggtgacg ttgttaccaa gcgtgatatc accaagaata atgacgttgg caccgagttc
    47701 gacgccgttg ccaatgtgtg gacatgccat gttatcggca ccacgattgc cgatagtgac
    47761 gccgtggcga atggtgaaat catcccccgc taccacgttt ttattgatca ctacggcgta
    47821 accgtgatgg atagtaaagc ggcggccaat ggtcgcggcg gcctggattt cataaccgaa
    47881 aaagcattcg gtgataatgc gatacagcac cagcagcggg gccgcccaca gattgttgag
    47941 gacgttcttt ttgcgccaca ccgaacaaaa atgagcaaca cgataggcaa gaaccatgca
    48001 gcacgggcgt aaactccagc tgttggcacg cagatcttcc agcatcttta gcgcccccga
    48061 ataccatcag ccagacgttt gccgttacgc accgacagca gcgtcagcaa agtgcgccag
    48121 gtcatacgtt tattgcggat ctggtagagg gtaaacagct gatatttttt gcttgcacgg
    48181 tcaaatttat ctttgtgctt tcggtaaaag tggaagtacc cggagaattt tttcggtgac
    48241 gaggtgatct gcatttcacc gtgattgata tgcaggatct gcgtcgcttc ttccactttc
    48301 cacggttcgc cgtactccac caccatccgc aggaagatgt cgtaatcctg tgctgctttc
    48361 agttcggtat cgaacaaaca ctctttgaaa cgccatgccc aagtaaagac ctgattgcca
    48421 atgatattgc gtttatagaa caggcggcgt gagtacggcg atttgggata cagtggtaag
    48481 cttgccggtt gggaataaac ttctccctgg catacgtagt cgttagcgta caaaaaggcg
    48541 tgagtgacca gttgctgttt atgggcgagg aagacgctca gacggttggg tgtccattcg
    48601 tcatcgtcat cgatcccggt aatgtattcc ccttgcgcca gcataatcgc ctggttacgt
    48661 accgcgcacg ccccgctgtt aatgtcgttg tgaatgtagg tgatacgcgg atcgttgagg
    48721 gcagtgacgt actgttgcag ttgttcccag gaggtggagc aatcatccac gatgatcatc
    48781 tcccagttgc tgtagtcctg gcgcaggacc gattttatcg cccgaatcgc cagttgttga
    48841 cggttccagg tcggcatata gattgaaatc agcggattct ctttcatagg ttgttctccc
    48901 ggcttatgag ctggtttgct tgccggatgc ggcgtgagcg ccttatccgg cctacggggc
    48961 ggtgcgaatg caggcgtcgt tatttcgcat ccgacttata ttcgtattcg taatagccat
    49021 aatcctgata cgcgctggcg cggcggaaga tggagttcag aatcacccct ttcaccggaa
    49081 taccgttttg ctcaaagcgg ctcagactgg tttccacttc tttcaatgtg ttgaccgcat
    49141 agcgcgccac cattaacgtg gttccagcgt gacgcccgac aattgctgca tcggtcactg
    49201 ccagaatcgg cggcgtatca atcaacacca ggtcgtagtt tttactcgcc cagctcacca
    49261 attcggcaaa gcgttcgctc atcaacagtt cagaaggatt tggcggcacc tgaccgcgcg
    49321 ggatcaggtc aaatttggca atagaggtcg gtttagctgc ggtagtaata tcgccctggc
    49381 caatcagaat ttccgacagg ccattgacgt tattggtacc caacagctcg tgggtgtagc
    49441 ctttgcgcat atcgcagtcg atcaacaaca cccgtttatt ggtctgactg atcaccgccg
    49501 ccaggttggc gcagacaaag gttttaccga ttgacgggct cacccctgtc atcatcaaca
    49561 cattgttctg cgcctgcatc atcgcgaagt gcagactggt acgcagactg cggatggctt
    49621 caatcgccag atcggttgga ttccccaccg ccagtagctg gctctgttta tagcgtttaa
    49681 tccctttgat ggttttgacg ctatcgcgcg ctttctgcca ttccgacagc gggatgctgg
    49741 catagacgct gataccatgt tcttccagca cctgcgggct ttcgatgccg cgattaaaca
    49801 acgagcgcag cagcacaccc acgatagaga gcatcaggcc gaggataatt gcgccgagga
    49861 taatcagtcc tttcttcggt ttcagcacgc caggctgggt gattgccggg tcaacaatgc
    49921 gcacatcgcc gacggtgctg gcttcggtga ttttcagctc ctgctcttta ttcagcagtt
    49981 gcatatagac ctgctgacca gactcgacat cgcgggtcag gcgcacaatc tcctgctggg
    50041 ttttcggcat cgccgttacg cgaccgttaa gtttggcttt ctcttcttcc agcgcctgac
    50101 gtttctccag cagcgtgcgg tacgccgggt gaactttggt gtacagcttg gagatttccg
    50161 cctctttaaa ggtcagttcg ttcaactgcg cgtcgatgtt caccatcgaa tcgagcaccg
    50221 ctttcgcttc cagcggcaga tcaacagaat ctttatcctg acggaaggca ttcagtttgt
    50281 tttcggcaac atcgaggcgg ctacgtactt ccggtaactg ttgcgcgagg aaagcgaggc
    50341 ttttcgacgc ttccgccgat ttgcgctcaa tattttgttc ctgatagtta cgggcgatgc
    50401 tgttaagaat gtcgcggatc tgttcgcgat cttcaccggt ataagtcagg ctcagtacgc
    50461 ctgcgtcttt gccgttctcc gttaccgtca ggctgttttg cagttggttg atcatcccca
    50521 acgtggagta tttggtgacg gtaaactcac tgcccgggcg ggcgtgaatg gcttcaacca
    50581 tcagcgtgac gccttctttt ttcagcatct ggcccgcttg cccacgggcg ctaaagccgc
    50641 catcactgct cagggtgtag tttttattgt ccagcacatt aagcgtaaac acctggcccg
    50701 ccatctcttt cgggcggtta aaggtagtca ctttcaccgt ctcgttctgg cgtcccatca
    50761 gtcgctccca gcccgccccg aaaatcggga acgtgttttt gctcactgca atatcgaggt
    50821 cgagatcgtc caccgtttta ccaagcacca gacgcgaacg aatcaactgg atctcggcgt
    50881 ccgatgcagg cggtttgttg gctaatgccg aaccaatgtc ctgcactaac gaattgccgc
    50941 tgttttgctc gatttgtacc agtgcgtcgg cactataaat cggcgttgcg aagaaggtgt
    51001 aaaccacggc acagagggca aatacagcgg tgatgccaat cacccaccag cgcgcttcaa
    51061 tgacggtgcc gaccaggcga ccaatatcga tttcatcact gcccgttacc ggagcggcat
    51121 gttgttttac tttttctgtc attcttatac ctgctctgcg ttcaatgcct gcgcccactg
    51181 gcgggcagac cgttcaagta atgtgtacac cgctgcaaac gtttcccggc ttttgcgata
    51241 cggatcgggg atttcacatt cgttatccca gtgaccaaac agcatcactt tgccgcgcat
    51301 ctcaggtgcc atctcgcata agcgttcgat atggcgcttt tccatggtca aaatcaggtc
    51361 atagttgcga cacaggcggc ggctgatttg tcgtgcacag tggccttcca gagacagttg
    51421 atgttctgcg gcgacgctga tagcggtagg atcagcgccc ttaccgacca gcgcgccgag
    51481 tccagcggac tccactttca gttccgggtg ataacgttgc agtaagcgtt ccgccgtcgg
    51541 ggaacggcaa atattgccga cacagacaac taatatgttg ttaaacatga cgattaccag
    51601 ttatgaatgt cgctggctgt atccgtcatg taacggacac cgctaatagt tggcaacaac
    51661 tgattgatca gacggttcca gcgggaaacc ggggcggtgg tgacatacac cacgtcataa
    51721 ggttgcaggc ggaattctgt cgccatcacc agcgacgtgg catcggacat atccagctgg
    51781 tagatattgg caatcttgcc attacgcccg ccctcgcctt tcagcggacg aatgacaaag
    51841 atgccgctgg cgttggaggt ggtcatgtcg ataccttcag cattacccag ggcttcagtc
    51901 agggtcatgc cgctaaagtc cattttcagg gtgctctgtt tcttcacttc acccatcaca
    51961 aatactttca gatcatcatt acgcggcacg tagagaatat cgccgggata aagcagtcgg
    52021 ttctgattga ggtcgccgtt ttgcatcagc gcctgcaaag aaatgcgttc ttcacgacca
    52081 ttgtgtgtta gcactacgtt gcgccagtca gcggtgtcgg tcaggccacc tgcggcgttg
    52141 atggcgtcga gaatagtcag tggcacgttg gtgatcgcct gttgaccgga tttattcacc
    52201 tgaccggaga tataggcctt ttgtgagcgg aaggcggcga tattaacgtc cacctgcggg
    52261 tcagcgatgt acgtcgctaa gcgcccggta atatcactgc ggatttcagc gagcgttttc
    52321 ccgactacgt gaaccttgcc gatatacggg taaaacatag tgccgtcagg ctgtacccag
    52381 ttgccggtgt cgctggagct gcggtactga ccggctggcg tggtgagttc cgggtgatcc
    52441 cagacggtga cattcagaac gtcgcccggc ccgacgcgat actggtaatt cgcgatctcg
    52501 ctttccagcg tcatattggg gcgcgctaca ttcgggcgtg ggcgtaattg gtcaatcagg
    52561 cgcggggtca gcggataaac attcaccatt ttgtcgagat cgaaatcagc gtcctgctgt
    52621 ttgatgacgt ctttgcccat cgtcgacata ttgctgcccg gaagtactgt gcaaccgctt
    52681 atcaaggtta ctgacaccaa taatggcatc aatttcattt tggatttcat cattgtttat
    52741 ttatcacttt ggcagagtaa ttatcctgtg cactattaat agcaatatcg ccatgcacat
    52801 ttaccttgca gttaattgaa taaaaattta actggcatca gtcctaaaaa aattgatttc
    52861 atccgcaggc tattgacaga ataattcaga ctggtctttc aggcatccag acacgctacc
    52921 gcccctggct ttttagccac caacacactg atttagctta atttttcaaa ccctctctgc
    52981 atacaatcgt tgatgagaaa gggttattgc ggaaattaac ttccgaatat aaggtgacat
    53041 tatggtaatt gaatattggc tctccaataa tgcaggagga agtgttacag ctaacggaat
    53101 agcaggcaag gtaacaattc ggcaattggc tatttttaag aattatatta agtgtcattc
    53161 aatatggttt ttaggagttt ctttaggttg acaatattta atattgcgcc tccacatgcg
    53221 atatttctta aataatgttt tattattacc acttttaatt cagggataat gtgaggttat
    53281 taccctcaaa taatatgaga taaatagtgc tgcaacattg catttttgcc ccgatttatc
    53341 catgatcgaa ttgtgacatt tgtcatacaa cgaataggtt ttgtacttac tatggaatgg
    53401 attgccgatc cgtctatctg ggccgggtta atcacgctga ttgtgatcga actggtcctc
    53461 ggcattgata acctggtctt tattgccatc ctcgccgaaa aactaccgcc gaggcagcgc
    53521 gaccgcgcac gggttaccgg gctgctgctg gcgatgttaa tgcgcctgtt actgctggcg
    53581 tcaatctcct ggctggtcac cctgactcaa ccgctgttca gcttccgctc gtttaccttt
    53641 agcgcccgcg acttaatcat gctgtttggt ggtttcttcc tgttgttcaa agccacgatg
    53701 gagctgaacg aacggctgga agggaaagac agcaataatc ccacacaacg caaaggtgcg
    53761 aagttctggg gcgtggtgac gcaaattgtg gtactggacg ccattttctc actcgactca
    53821 gtgattaccg ccgtcgggat ggtcgaccat ttactggtca tgatggccgc cgtggttatc
    53881 gctatcagcc tgatgttgat ggccagcaag ccgttaacgc aatttgttaa cagtcacccg
    53941 acgatcgtta ttctctgctt aagcttcctg ttgatgattg gctttagcct ggtggcagaa
    54001 ggtttcggct tcgtcattcc gaaaggctac ctgtacgctg ccattggttt ctcggtgatg
    54061 atcgaggcgc tcaatcagtt ggctatcttt aaccggcgac gttttctttc cgctaaccag
    54121 acgctgcgcc agcggactac cgaagcggta atgcgcctgc ttagcgggca aaaagaagat
    54181 gcggaactgg acgccgaaac cgcgtccatg ctggtggatc atggtaacca gcagatcttt
    54241 aatccgcagg aacggcggat gattgagcgg gtacttaatc ttaaccagcg taccgtcagc
    54301 agcattatga cgtcgcgcca cgatattgag catatcgatc tcaacgcgcc ggaagaagaa
    54361 atccgccagt tgctggagcg aaatcagcat acgcggctgg tggttaccga cggtgatgac
    54421 gcagaagatt tgctcggtgt tgttcacgtt atcgacctgc tacaacagtc tctgcgcggc
    54481 gaaccgctca acctgcgggt gttgattcgc cagccgctgg tgttcccgga aaccttgccg
    54541 ttgttacctg ccctggagca gttccgtaat gcccgcacgc actttgcttt tgtggtggat
    54601 gagtttggct cggtggaagg gattgtgaca ttaagtgacg tcactgaaac cattgtcggt
    54661 aacttaccga acgaagtgga agagatcgac gcccgccatg atattcagaa gaatgcggac
    54721 ggttcctgga cggcgaatgg tcatatgccg ctggaagatc tggtgcaata tgtgccgctg
    54781 ccgctggatg aaaaacgtga atatcacacc attgccgggc tgttgatgga atatttacag
    54841 cgtattccaa aacctggcga agaagttcag gtgggggatt atttgcttaa aacgttgcag
    54901 gtggaaagcc atcgcgtgca gaaggtgcag attattccgc tgcataagga tggcgagttg
    54961 gagtacgagg tgtgatggta tcggtcattt tacccaatag ccgaaaggaa taagatgagg
    55021 gaaccgcgcc agcggcgatt taccgccggg agcccgggtt gccagggtgg tggcgattga
    55081 gccaccctgg cacgttcacc ggcttgagcg ttccatagta gcgaggaaca tcaagtgaac
    55141 ggaacagcct tcgatcttac atattcagaa tataatgatg ccgccacgaa cgcctgatgc
    55201 gacgctttac gcgtcttatc aggcctacag gactggtcac ctggtaacct acatcttctc
    55261 cagcaacttc ttcacatcct tgccattgcg ggaatcttta ttccgctcgg cccagtcatt
    55321 caggcgacgt ttcgcttcgt cctgtagatg tttgcgcagt aactgatcca cttgcaggct
    55381 gtaattgagt tgctgccagt tgccataaac ccgcagcggt actggcgttt ctttcaggaa
    55441 atcaatcagt ttactttccc cgttccagcc acccacgacc cgaatatcaa actgggtatc
    55501 gcaggtttga tctgccagat tcaacatgcc ttcccctgtc agcgccagca caggcgattg
    55561 accttgcatg tcgtttaacg tcacgacgcc atccttcaac gtcaaatcgg tggtaaagcg
    55621 gtcaagccgc gtcacgttat caaagttttc agcggccttc acatcaccgc cattacgctc
    55681 taccgcttgc tgaatcatct gctggaagtt catcccttcc atgcgcgtgt cggtcatttc
    55741 gacatgtgcc tgtccttgcc agttgtggcg gaatgcgtcg gcatctatgt cagcaccgga
    55801 gaaatcacca gccagtgaca tttttccggt caacgaaatc ggatagttaa acgccttcag
    55861 aatggtacca atctcaacgt tttccagccg tggctggaag tttatccgcg gatttattga
    55921 tgtcgcgtcc agcgtgcccg gcagtgaaac ctgtccaccg ttaagtttgc cctgcagttg
    55981 agtaatttcc agcaaacccg acttgttggt catttgcgtg gcaacatctg taaaattcat
    56041 tccgcgccag cgcacgttac tggcctgcaa caaaatatca gccgtaaagc cttgcagtcc
    56101 ctgataggcc ggttcatcaa tacgcgaaga aatgaccggg cgcggcaacg ttgattggct
    56161 ctgcccctgc tgcgcggcac cgttttcacc attcgcggtt tcattaagcg ggatgaggtt
    56221 gtcaagattc agttgcggga attgcagtct cagctgccat tccggtttct ctgtcagcgt
    56281 gacctgtgct tgcccgctca gcgtactgtc attggcggtc aagctgattt ggttaaatga
    56341 aaggcgttta tgtgactcct gccactgggc ctggaaactc ccctgcccct gaataccttg
    56401 ttttggtaaa tcggcaccct gcaactgcca gttaatttgt tcaatagccg ccgttaaatc
    56461 atgcggataa tcagaagcat ccaccgtacc gttaagggat attgtgagat cgcgctgatc
    56521 gcgattaacc cgcccggaga actcaaatga gccacgatgt tggggatctt gttccatttg
    56581 caggcggata ttgcggattg tcacctgctc gtcatcttca tgctggaaca ccagcacgct
    56641 gtccgccacc ttaagactgg atatatcaaa cgaccatccg cgatcgtctg acagatccgg
    56701 caaggtattg tcgcgcggtg caaccggagc gtcttcactg cgcaccgctt ccgtctgcgg
    56761 cgtcagttgg atcactgccc cttttagcat cacctgctta acgctcagtt gatgactcag
    56821 tagtggtaaa agcgccacgt ccagacgcat gttgtcggcg cgaaccagtg gctggcttgc
    56881 gccctgggcg gtgagagaca ttcgcccgga gaggatacta agctgcggcc agacgtgcca
    56941 acgcagtggc ccgtcgagct gcaattgata accgctacgt gcagcaactt gcttgaccat
    57001 atagtcgcgg aaatcattcg gattcaccag caacactaac acagataacc cggccaccag
    57061 cacgaccagg agtatcatca gcgtcgtcag aaatcgtctc atggtatcct caatgggcct
    57121 gaattagtct ttatcgattc ggctggctac cgcgccctgc tggttgcgat atttcgcatc
    57181 ttcacggcgg ttgtaaggtc gcgccgccgg gccggaaagc ggctcaaagc tcagcgcacc
    57241 aattaacatg cccggacgca gcgccagcgg cagcttaccg gagttgtaga actccagcac
    57301 aatgcaacca gaccagcccg gatcgatacg gtgcgcggtg acgtgcacca tcagccccag
    57361 acgcgccaaa gaggaacgcc cgtccagcca gcccaccaga tcggctggca gcgtcaccga
    57421 ctccagcgtc accgccagcg ccagctctcc tgggtgaaga tagaacgcct cgccctcgtc
    57481 gagaacgatc tcatcgctca tcacgcggtc aagcgcggcg ctcacttcat ctttgggacc
    57541 gctcagatcg ataaacgctg ccgtgtgacc acggaaggta cgaaatttat tgcccaggcg
    57601 tacatccacc gtcgcgccgt taatacgctc cactggcgga cgtgggttga tcgacaaacg
    57661 gccttcatca agccaggctt caatatctcg gtcacacaga cgcatggcat tttctccttt
    57721 cgcgcatcac tcccttaacg ccaattacgt caagggcata ccaggttatc actgaacggt
    57781 acacaattta tcaagcttat tcaaagaact gacttatttt cgctttcaat atatcgatcg
    57841 cgatgcggtt tttcccgccg cgcggcacga taatgtccgc atattgttta gaaggctcaa
    57901 tgaattgcag gaacatcggg cgcacggttt tttgatattg cgccatcacg gaatccattg
    57961 aacgcccacg ctcgttaacg tcacgcttga tgcggcgcat caggcagata tccagcgggg
    58021 tatcaacgaa aatggagaag ttaagttcgt cacgcaaacg cgcatccgtc agcaacaaaa
    58081 tgccttcgag aatgatgacc ttcttcggct caaccgtcac cgtttctttc atacgcgtat
    58141 gttcaacata gctgtaaacc ggcaggtcaa ttgccgagcc gcgtttcaac gcttgtaaat
    58201 gctcaagcag caggctgtga tccatcgcgc tgggatggtc gtagttggtc ttaacgcgtt
    58261 cttccatcga cagatggctt tgatctttgt aatagcagtc ttcgggaatt acgccgatgt
    58321 gttcatcacc gacttgctca cgtaattcac gataaagggt actggcaata agactcttgc
    58381 cggaagccga tgcgccagcg ataccgataa tgacgcactg atgagactga tcagtcatat
    58441 atttagcgac ctgattaacc tggatgttag gaagggggcg acgaagcgcc aaacgcggca
    58501 attataggga tttcatccgt ctgataccag tcgaatagcg ttgccgcgcg ctcagagtta
    58561 attgttgaca aagaattccc ggtggcaaat tacgttgatc agttttatgt aaggtaaaaa
    58621 atgttatacg cagttgcgca aattatccgc ctttacgtca ctttatgagc aattcgcata
    58681 taaaatgtaa aacttttgta ctagcataaa cacagaaacg aatactggcg acctggtctt
    58741 gcggataaag cggtaatgag caaacaatca cagcatgtat taattgccct gccccacccg
    58801 ctgcttcacc tggtcagttt aggtttagtc tcgtttatct ttaccctttt ctcgcttgag
    58861 ctttcgcagt ttggcaccca actcgcccca ctgtggttcc cgacgtccat catgatggtg
    58921 gcgttttatc gccatgccgg gcgcatgtgg ccgggaattg cgctgagctg ttcgctggga
    58981 aatatcgccg catccatcct gcttttttcc accagctcgc tgaacatgac ctggacgacc
    59041 atcaatattg ttgaagccgt ggtcggggca gtgctgctac gtaaattgct gccgtggtat
    59101 aaccccttgc aaaatctggc tgactggctg cgtctggcac tcggcagcgc cattgttccg
    59161 cctctgttgg ggggtgttct ggttgtcctg ctgacgcccg gagacgatcc tctcagggca
    59221 tttttgatat gggtactgtc agaatccatc ggcgctctgg cactggtgcc gctgggattg
    59281 ttatttaaac cacactatct gctgcgccat cgcaacccac ggttgctttt tgagtcgctg
    59341 ctcacgttag ccatcacact gacgttaagc tggctttcga tgctgtatct gccgtggcct
    59401 tttactttca ttattgtgct gttgatgtgg agcgccgtgc gcctgccacg aatggaagcc
    59461 tttttgatct tccttaccac ggtgatgatg gtgtcgctga tgatggccgc ggatccctcc
    59521 ctgcttgcta cgccgcgtac gtacctgatg agccatatgc cgtggctacc gtttttgctg
    59581 atcctgctgc ccgccaacat catgaccatg gtgatgtatg cctttcgtgc ggaacgcaaa
    59641 cacatttccg aaagcgaaac ccgctttcgg aacgcgatgg aatattccgc tatcggtatg
    59701 gcgttagtgg gcaccgaggg acaatggctg caaaccaaca aagcgctctg ccagtttctc
    59761 gggtacagtc aggaagagct gcgcggactc acctttcagc aactgacctg gccggaggat
    59821 ctcaataaag atctccaaca ggttgaaaag ctgataagcg gtgaaataaa cacctattca
    59881 atggaaaaac gctactacaa ccgcaatggc gatgttgtct gggcgttgct tgccgtctca
    59941 ctggtgcgcc acacggatgg cacgccgctc tattttatcg ctcagattga agacattaac
    60001 gagctaaaac gcaccgaaca ggtcaatcag caactgatgg agcgcatcac gctggctaac
    60061 gaagcgggcg ggattggcat ctgggagagg gagttgaagc cgaatgtttt tagctgggat
    60121 aagcggatgt tcgagctgta tgaaattcct ccacatatca aaccgaactg gcaggtgtgg
    60181 tacgagtgcg tgctgccgga agatcgccag catgccgaaa aagtgattcg tgattcgttg
    60241 caatcacgct cgccctttaa actggaattt cgcattaccg taaaagacgg tattcgccat
    60301 atccgcgccc tcgccaaccg ggtactgaat aaagaaggcg aagtcgaacg tctcctcggc
    60361 atcaatatgg atatgaccga agtgaaacag cttaacgagg cattgtttca ggaaaaagag
    60421 cgcctgcaca ttacgcttga ttccatcggt gaagccgtgg tctgtattga tatggcgatg
    60481 aaaattacct ttatgaatcc agtggcggag aagatgagcg gctggacgca ggaagaagcg
    60541 ttaggtgttc cgctcctgac ggtgttgcat attacttttg gcgacaacgg accattaatg
    60601 gagaacattt acagtgccga cacctcacgt tccgcaatcg aacaagatgt ggtgttgcac
    60661 tgtcggagcg gcggcagtta cgacgtgcat tacagtatta cgccgttaag tactctggac
    60721 ggcagcaata ttggttcggt tctggtgatt caggacgtca ccgaatcacg caaaatgctg
    60781 cgccagctga gctacagcgc ctcccatgat gcactgacgc atctcgccaa ccgcgccagt
    60841 tttgagaaac aactgcgtat cctgctgcaa acggtaaaca gtacgcatca gcgacatgcc
    60901 ctggtgttta tcgatcttga tcgctttaaa gcggtgaatg acagcgccgg gcatgcggcg
    60961 ggcgacgctt tgctgcgcga actggcgtcg ttgatgctga gtatgctgcg ctccagtgac
    61021 gtgctggcgc ggctcggcgg tgatgaattt ggtctgctgc tgccagactg caatgttgaa
    61081 agcgctcgtt ttatcgctac acgtattatc agtgctgtga atgactatca ctttatatgg
    61141 gaaggccggg tacatcgggt aggtgccagc gccgggatta ccttgatcga tgacaacaat
    61201 catcaggcgg cagaagtgat gtcgcaggct gatatcgcct gttatgcctc caaaaatggt
    61261 ggacggggcc gggtgacggt ttacgaacca cagcaagctg ccgcacatag cgaacgggcg
    61321 gcgatgtcgc ttgatgaaca gtggcggatg attaaagaga atcagttgat gatgatcgcc
    61381 cacggtgtcg cttcgccgcg gatcccggaa gcgcgtaatt tgtggctgat ttcgcttaag
    61441 ctctggagtt gcgaaggcga aattattgat gaacaaacat ttcgtcgtag cttcagcgat
    61501 ccggcactta gccatgctct tgaccgacgg gtattccacg aatttttcca gcaggccgcc
    61561 aaagcggttg ccagtaaagg cataagcatc gccctccccc tttccgttgc cggtttgagt
    61621 agcgccacgc tggtgaatga tctgcttgag cagctggaaa atagccctct accaccacgg
    61681 ttattacatc tgattattcc ggctgaagcg attttagatc acgcagaaag cgtgcaaaaa
    61741 ctgcagctgg cgggatgtcg gatagtgctc agccaggtgg gccgcgatct gcaaatcttc
    61801 aactcgctga aagcgaatat ggcagattac ctgctacttg atggtgagtt atgcgccaac
    61861 gtgcagggta atttgatgga tgagatgctg attacgatta ttcaggggca cgctcagcga
    61921 ctcgggatga aaaccatcgc cgggccagtc gttttaccct tagtgatgga tacgctttct
    61981 ggcatcggcg tcgatctgat ttatggtgat gtgattgccg atgcccaacc gctggatttg
    62041 ctggtgaata gcagttattt cgcgattaac tgagagagcg tttgcccatt gcctgatgcg
    62101 acgctaacgc gtcttatcat gcctacaaat cgctcattcc ccagaccgga taaggcgctc
    62161 gcaccgcatc cggcgaccaa cgtcatgctt cgtctggttg ccagccttcc gtataccaga
    62221 tatgcaacag cgcataagaa cgccagggct tccagcgctc ggcataacgg cggatttgtg
    62281 ccggtgtcat tcccggaaat cgccgtttaa tcagataatc atccggcaga aaaacatctt
    62341 tcgcctgcca gccacgcaaa gcaaaataat tcgccgtcca gcgcccgata cccggaaaag
    62401 tttgcagcgt tttcatcgcc tgctccacat cgcccggaat tgtcattggt aagctgccct
    62461 ccagcgccgc atttgccaga tgaatcagtg cctctgcccg tttcaacggc atgcctaacg
    62521 cttttaatgc ctgcgggtcg gctgctgcca gccgctgagg cgtggggaag cagatatact
    62581 ccggaaaatc atccagccgt tcgccataaa gctgtaccac tttggcagtc aattttgccg
    62641 ccatcgccac gctcaccagt tggcctaaaa tcgcccgcac accctgctca aaagcatcaa
    62701 tacagccggg taaacgcaat ccgggccgcc ccgcgcctaa ctttcccaac gcaccgttaa
    62761 caatctgcgg gttacattgc agatcaagca ggcggctcat tttcgccaga cactctgcgg
    62821 caacaggttc taaacctgca cttaaattta tgtgcagagt atggcgggct atatccggaa
    62881 tagcagtcac tacgccgcga tattcgccca ccgccagact acgggcataa taattgtccg
    62941 cgaccgtttc cacaccgctc accgcacggg cggcgagaaa tcccaacatc cacgaccagt
    63001 catacggcgg ctgccagttc agggtataca tcgcatctcc ttgttaatcc gctttcagca
    63061 taaacgttat tcagacgtta cgctttgctt tcatattccg gttgtcgcga cagcaacatt
    63121 tcgctaaagt cacgcccctt cttcaccggc atggggatta attcgtgttt attggttttg
    63181 attacggtac agcaaactgt tcagtggcgg tcatgcgtga cggtaaaccg caattactga
    63241 aaatggaaaa cgacagcacg ctgctgcctt caatgctttg cgcgccaacg cgtgaatcgg
    63301 taagcgaatg gctgtaccgc catcatgatg ttccggcaga cgacgatgaa acgcaggcgc
    63361 tgttacgtcg ggcgattcgt tataaccgcg aagaagatat cgatgttacg gcgaaaagcg
    63421 tgcagttcgg tctttcctca ctggcacagt acattgatga tccagaagaa gtgtggtttg
    63481 tgaaatcacc aaaatcgttc ctcggtgcca gcggcttaaa accgcagcag gtagcgctgt
    63541 ttgaggatct ggtctgcgca atgatgttgc acattcgcca gcaggcgcag gcgcagctgc
    63601 cagaagcgat tactcaggcg gtgattggtc gcccgatcaa cttccagggg ctgggcggcg
    63661 atgaagcaaa cgcccaggcg caagagattc tggaacgcgc ggcgaagcgt gccggattca
    63721 gggacgtggt attccagtac gagccgatcg cggctgggct ggattacgaa gccaccttgc
    63781 aggaagaaaa acgggtgctg gtggtggata tcggcggtgg tacgactgac tgttcattgc
    63841 tgctgatggg gccgcagtgg cgttcgcgtc tcgatcgtga agccagtctg ctgggtcaca
    63901 gtggttgtcg tattggcggt aacgatctgg atatcgccct ggcgtttaaa aacctgatgc
    63961 cactgctggg tatgggtggc gaaaccgaaa aaggcatcgc cctgccgatc ctgccgtggt
    64021 ggaatgcggt tgccatcaac gacgtacctg cgcagagtga tttctacagt agtgccaacg
    64081 gtcgtctgct taacgatctg gtacgcgatg cccgcgaacc ggaaaaagtg gccctgttac
    64141 agaaagtctg gcgtcagcgt ttaagctatc gcctggtgcg tagcgcagaa gagtgcaaaa
    64201 ttgctctttc aagcgtagcg gaaacccgcg cctcactgcc gtttatcagc aatgaactgg
    64261 ctacgctgat tagccagcga gggctggaaa gcgccctcag ccagccactg gcgcggattc
    64321 tggaacaggt gcaactggcc ctggataacg cccaggaaaa accggacgtt atctacctga
    64381 ccggcggtag cgcccgttct ccgcagatta aaaaagcgct ggcagaacag ttgccgggca
    64441 ttccgattgc aggcggcgat gactttggct ccgtcaccgc cgggctggca cgctgggcgg
    64501 aagtggtgtt tcgttaattg aaaaatggct cagcagcggc ttaaatctgc ctgcgcctga
    64561 gccagttttt ctgctgcctg acgtaacggt tccatcaacg gcatggtccg tgctggagca
    64621 tgaaggcgga atcgctgcaa ctctgtcgcg ccgactgtca acgccgattc cagccgatgt
    64681 cgtttggcgg tcattttcgc cattaccgct tgtctgtctg ctggcgttat cgcttcattc
    64741 ggcctgaaca caaaacggcg ttcacagttc gctttccagt cgataaccgc ctgggtcaga
    64801 tggtcgccaa accctttcac ttgcttaacg ccacgacggg taacatctgc ggctgtttca
    64861 ataccaaaag accgtaacgc cgctttacgc gcagggccaa cgccgggaat agaggcaaca
    64921 tcaataaaaa atccctccag aaacttctgc ttctgccgtt cccttgcagt gtcatgaagt
    64981 gctgccagag cgcgtttctc ttcttccggt aacccgagaa tttcgtcctt cattttttcg
    65041 agcatcgtcc gtttggcgat aaaaccttcc aggccgccta cctgctggat ctggcggact
    65101 aaatggtcat agtccatttt cgcacgatta aatgcctcac ggcgttgctg aacttctgct
    65161 ttgtacgctt tgcttgtcaa actgccaata atccagatag ccgccagcac aggaaccaaa
    65221 ataatataac gcggttctgc ctgaaggccg caaagcagca gcgataatgc tgacagtgcg
    65281 atctcaagca gaatgatgta ttcgcgccgt aacaggccta aaggaagcgg cctgccagtc
    65341 ggttggaaat gatcgggtaa tggcagttgc aatgctggcg gtgctactga cgccatcacc
    65401 atcgcccaga cttttgccag cacaaaatcc ccgccggtgg taatgacctc ttcgccgaga
    65461 tcaataaaat agataacacc ttgattatcc agcgcacacc atgggcagtc agccaaatga
    65521 ccgggataaa catgcattgc cgaaacggta catttcttta actgttggcg tagagaatcc
    65581 agtgctgata cccacgcttt agccgtcgga cgcccggttg ccacgccact ttccgtaaac
    65641 gcctgctgaa acatggcttc aacatcactc ggtaacatcg atagcggaat cgatcgtggc
    65701 ggcggtttta aaccacgtcg ctgattatct gacgcgtagg cataacggaa atgggtaata
    65761 tccgtctcca gcgcattacc cgcatcagag ataagcggca caccagaata aggatgccgg
    65821 ccaccaaaca agacgtgaaa aatcagcaac gcaaggccaa aattatcgtg attttctgtg
    65881 cgttcaaagc cgacaaatga cgacaaggtt tgtagctctg gcggcgtaaa atgcgacacg
    65941 ccgacttcgc ataaatgcag tgtgccattg gcgttaatct gaaaggagtc gctatcaatc
    66001 aacaccactt tgctgtcgcg acctaccata aaactgttct ggtttacgtc acccacgacg
    66061 tgcccgtgct cgtgaaccgt agcaaaagat gaagcaatat tgcgcgcaac atagagtaga
    66121 aaatcccacg cacaatgagg gtaacgttga cgacgatgtg ccgggctata gatcatatga
    66181 atcggttctt taccagaaac ttttggcatc ataaaaccga taacttttcc gcctcgtcca
    66241 ccgtgaagcg ttgcctgcgg ccaggcgaca taattcaaca attgcgcgtc agctgtcgca
    66301 gccataaagg caagtttgtc ctgttttaag gcgggtggcg gcgtgtgata aatcttggcg
    66361 acgctatcga caaactcatt gatatcataa accgcgcctt caccgccttt gcccagttca
    66421 cggcctggcg taacgcactc accagtagca gtatataaag tgggtttcat ggtaaccaca
    66481 gcgccagtgc caacgtttta tcatcatccg tacgctcgtt taccgccggg ctggacaaaa
    66541 actgctttaa caattcaggt aataaatcga gttgttcctg cgttgctgac gccagtccat
    66601 tgaaaaacgg ggtaaagaaa ggcacatgag gagaattatc cagcatattt aacgccagcc
    66661 gctgaatacc atccgtaaat gctgcaactt tatgcgcacg cccagtgctg gtgaaagttt
    66721 ccagtctgga aacggcatct tcgtcggtaa tgaagtgcgt catgttggca tattcaccca
    66781 ccatcggcgt gagaggcaat tgcagaccgt gacccaaatc taccaccacg ccgccatcgc
    66841 caatctgcat aatcaaggtg ccgtcaggcg atgaaatcaa tcccagaaac gtgcaggcaa
    66901 aatcgcgaac tgctaatgct ttggcttctg cctcggcaaa aagtcgttga cgaatagtca
    66961 gtaccatatt tgtggcgagg acatcattaa gccccaattc accgtcctgt actttttgca
    67021 ccataaacgc catcgcttca ttgacggcga gcgtcgcgcc ttctcctccc tgcgaaacgc
    67081 tacctgcgcc atcagcaagg aacatcacca gtaatggttg ctggtcgttt aaccagacaa
    67141 cctgcatctg gcaggcatcc tgacaaggca aatcagcact aatgtgcgat gttccaacag
    67201 cggatgcata aaccagacgc cagctcacac tgacgtccag ccttttggcg cttccagcac
    67261 aacttccgtt cccggcgtgg agcgggaaac cgaacgcagc gaactggata accagctaaa
    67321 cagttcacgg aattgtaatc cctgcagtgg caaaggctga cgaacgctga tttgtgccag
    67381 agtcttcata tccgccccct gaacaccgat ggaaaagaag gcaaacttct tatcttcttc
    67441 cccctgaaac actttgttgg cggcagcctg ccactcatcg gttggagcgc catcggtgat
    67501 caggaaaatc cacgggcgat aatacgaaat accatttgcg cggtactcac gtttcctctc
    67561 ttcaaccata tccagggctt tggtaatggc ggcccccata ggcgtatccc cctgggcaaa
    67621 caggatgggc gggaaaaagt tagcggcgct ggtaaagggt tgttccacat gcaccgggcc
    67681 gaacgtcaca atgccgagtt ccactctttt taaagccagc ggatcagcaa gcagttcatc
    67741 acgaaaggta accaatccgg cgttaagttc attaataggt ctgccgctca tcgagccaga
    67801 aacatccagc agcaaaatgc aggggcaacg tggttccggg ttactggcaa aatcgctggt
    67861 ggcaaacgtg atttgttcgc tcataagaaa tcctttctta gagaattatc gaggtccggt
    67921 atcataccga ttgcaataat gaaataagag caaacgaaga aactttcaga attaggcccg
    67981 tcggcgcaga aaattttgct attcgcctcg caagaacgga gagccgtcga ttgacactaa
    68041 gggcggagtg acataatttc aggagtgagg gttagggaga ggtttccccc tccccctggt
    68101 gttcttagta agcctggaag ctaatcacta agagtatcac cagtatgatg acgtgcttca
    68161 tcataaccct ttccttatta aaagccctct tctccgggag agggtttccc gtttcagcgt
    68221 cccgctgaaa tcgtcggctt acctcctttc gccctgaatg cagtctatcg ctaacgcgtg
    68281 aacaaaatag tttcatctgt gattattgcg taacgactcg caaagaatat ttgctggcag
    68341 gatcgcagac tacaaagcct gcggattgac aatcttatcg tgaaggcata atttcaggag
    68401 tgagggtaga gcggggtttc ccccgccctg gtagtcttag taagcgggga agcttatgac
    68461 taagagcacc acgatgatga gtagcttcat catgaccctt tccttattaa ggccccttcc
    68521 tcgggagggg ctttcccgtt tcagcgtccc gctgaaatcg tcggcttacc tcctttcgcc
    68581 ctgtatgcag tctatcgctg acttgcggat aaaatagttt ccagtgtgat tattgcgtaa
    68641 cgtctcgcaa agaatatttg ctggcaggat cgcagactgc aaagcctgca gattgacaat
    68701 cttatcgtga aggcatactt tcaggagtga gggtagagcg gggtttcccc cgccctggta
    68761 gccttagtaa gctggccagc taatgactaa gagcaccacg atgatgagta gcttcatcat
    68821 gaccctttcc ttatttatgg ccccttcctc gggaggggct ttcccgtttc agcgtcccgc
    68881 tgaaatcggc ggcttacctc ctttcgccat gcaagcagtc tatcgctaac gcgtagataa
    68941 aatagtttcc tgtgttatta ctggatgcgt gctcgcaaat gtgcccgtca ttcagacgat
    69001 tcccgacagt gtttcataat tcctccattt ttctccctta ttggctggct acactagtat
    69061 cattccgcga aacgtttcag gaagagaaac tcttaacgat gaaaggcagt tataaatccc
    69121 gttgggtaat cgtggtggtt atcgccgcca tcgccgcatt ctggttctgg caaggccgca
    69181 atgactccca gagtgcagcc ccaggggcga cgaaacaagc gcagcaatcg ccagcgggtg
    69241 gtcgccgtgg tatgcgttcc ggcccattag ccccggttca ggcggcgacc gccgtagaac
    69301 aggcagttcc gcgttacctc accgggcttg gcaccattat cgccgctaat actgttacgg
    69361 tgcgcagccg cgtggacggt caactgatgg cgttacattt ccaggaaggc cagcaggtca
    69421 aagcaggcga tttactggca gaaattgacc ccagccagtt caaagttgca ttagcacaaa
    69481 cccagggcca actggcaaaa gataaagcca cgcttgctaa cgcccgccgt gatctggcgc
    69541 gttatcagca actggcaaaa accaatctcg tctcccgtca ggagctggat gcccaacagg
    69601 cgctggtcag tgaaaccgaa ggcaccatta aggctgatga agcaagcgtc gccagcgcgc
    69661 agttgcaact cgactggagc cgcatcaccg caccagtcga tggtcgcgtt ggtctcaagc
    69721 aggttgatgt tggtaaccaa atctccagtg gtgataccac cggaattgtg gtgatcaccc
    69781 agacgcatcc tatcgattta gtctttaccc tgccggaaag cgatatcgct actgtagtgc
    69841 aggcgcaaaa agccggaaaa acgctggtgg tagaagcctg ggatcgcacc aattcgaaga
    69901 aattaagtga aggcacgctg ttaagcctcg ataaccaaat cgatgccaca accggtacga
    69961 ttaaagtgaa agcacgcttt aataatcagg atgatgcgct gtttcccaat cagtttgtta
    70021 acgcgcgcat gttagtcgac accgaacaaa acgccgtagt gatcccaaca gccgccctgc
    70081 aaatgggcaa tgaaggccat tttgtctggg tgctgaatag cgaaaacaag gtcagcaaac
    70141 atctggtgac gccgggcatt caggacagtc agaaagtggt gatccgtgca ggtatttctg
    70201 cgggcgatcg cgtggtgaca gacggcattg atcgcctgac cgaaggggcg aaagtggaag
    70261 tggtggaagc ccagagcgcc accactccgg aagagaaagc caccagccgc gaatacgcga
    70321 aaaaaggagc acgctcctga tgcaggtgtt acccccgagc agcacaggcg gcccgtcgcg
    70381 cctgtttatt atgcgtcctg tcgccaccac gctgctgatg gtggcgatct tactcgccgg
    70441 gattatcggt tatcgcgccc tgcccgtttc ggcgctgccg gaagtggact atccgaccat
    70501 tcaggtggtc acgctctacc caggtgccag cccggatgtc atgacctctg ccgttaccgc
    70561 gccgctcgaa cgccagttcg ggcagatgtc tggcctgaaa cagatgtcgt cgcaaagttc
    70621 cggcggtgcg tcagttatca ctttgcagtt ccagctaaca ttaccgctcg atgtcgccga
    70681 gcaggaagtg caggccgcga ttaacgctgc gaccaacttg ttgccgagcg atctgcctaa
    70741 cccgccggtt tacagcaaag tgaacccggc agatccgccg atcatgacgc tcgccgtcac
    70801 ctcaaccgcc atgccgatga cgcaagtgga agatatggtg gaaacccgcg tcgcgcagaa
    70861 aatctcgcag atttccggcg tcggcctggt gacgctttcc ggcggtcagc gtccggctgt
    70921 tcgcgtcaaa cttaacgctc aggcgattgc cgccctcggc ctgaccagcg aaaccgtgcg
    70981 caccgccatt accggcgcta acgttaactc ggcaaaaggt agcctcgacg gcccttcccg
    71041 tgcggtcacg ctttccgcga acgaccagat gcaatccgcc gaagagtatc gccagctaat
    71101 catcgcctac cagaacggcg cgccaattcg tctgggcgat gtcgcaactg tagagcaagg
    71161 tgcagaaaac agctggctcg gcgcgtgggc gaacaaagaa caggccattg tgatgaatgt
    71221 tcagcgccag cccggtgcta acattatctc caccgccgac agcattcggc agatgctgcc
    71281 acagctcact gagagtctgc cgaaatcggt gaaggtgaca gtgctttccg atcgcaccac
    71341 caatatccgc gcatccgtcg atgatactca gtttgaattg atgatggcta tcgcgctggt
    71401 agtcatgatt atctacctgt ttttgcgcaa tattccggcg accatcattc ccggtgttgc
    71461 tgtaccgctg tcgttaatcg gcactttcgc ggttatggtg tttctcgatt tttcaatcaa
    71521 taacctgaca ctgatggcgt taactatcgc caccggattc gtggtcgatg acgccatcgt
    71581 ggtgatcgaa aacatttccc gctatatcga aaaaggcgaa aaaccgttgg cggcggcgct
    71641 caagggcgca ggtgaaatcg gctttaccat tatctcgctg accttctcac tgattgcggt
    71701 gttgatccca ctgctgttta tgggcgatat cgtcgggcga ctgttccgcg aatttgctat
    71761 taccctggcg gtagcgattt tgatctcagc ggtggtgtcg ctgaccctga caccgatgat
    71821 gtgcgcgcgg atgctcagcc aggagtcgtt gcgtaaacag aaccgcttct cccgtgcctc
    71881 ggaaaaaatg ttcgacagga taatcgccgc ctatggtcgt ggactggcga aagtgctgaa
    71941 tcatccgtgg ctgaccttaa gcgtggcact cagcacgctg ctgcttagcg tgctgctgtg
    72001 ggtgttcatt ccgaaaggtt tcttcccggt acaggacaat ggcattattc agggcacttt
    72061 gcaggcaccg caatccagct cctttaccaa tatggcccag cgacaacgcc aggtcgcgga
    72121 cgtgattttg caggatccgg cagtgcaaag cctgacctca tttgttggcg ttgatggcac
    72181 taacccgtcg ctgaatagtg cacgtttaca aatcaacctc aaaccgttgg atgaacgtga
    72241 tgatcgggtg caaaaagtca tcgcccgtct gcaaacggcg gtagataaag tgccgggcgt
    72301 cgatctcttc ctgcaaccaa cgcaggatct gactattgat actcaggtca gccgcaccca
    72361 gtaccagttt accttgcagg ccacctcact ggatgcgctc agtacctggg tgccacagtt
    72421 gatggaaaaa ctccagcaac tgccacagct ttctgatgtc tccagcgact ggcaggacca
    72481 agggctggtg gcgtatgtca atgttgatcg cgacagcgcc agccgtctgg ggatcagcat
    72541 ggcggatgtc gataacgccc tgtacaacgc gtttggtcag cgactgattt ccactattta
    72601 tactcaggct aaccagtatc gcgtagtgct ggagcacaac accgaaatta ccccgggcct
    72661 cgcggcgctg gataccattc gcctgaccag tagcgacggc ggcgtggtgc cgttaagctc
    72721 aattgccaaa gttgaacagc gttttgcgcc gctttccatc aaccatctgg atcagttccc
    72781 ggtaacgacc atctccttta acgtgccgga taactattcg ctgggcgatg cggtgcagga
    72841 gattatggac accgaaaaga cgctgaatct gccggtggat atcaccacgc aattccaggg
    72901 cagcaccctc gccttccagt cggcgctggg cagcactgtc tggctgattg tcgcggcggt
    72961 ggtggcgatg tatatcgtgc tcggcattct gtacgagagc tttattcacc cgatcaccat
    73021 tctctcgacg ctacccaccg caggggtcgg cgcgctgctg gcattaatga ttgccggtag
    73081 cgaactggat gtgattgcga ttatcggcat tattttgctg atcggtattg tgaagaagaa
    73141 cgccatcatg atgatcgact tcgcgctggc tgctgagcgc gagcaaggca tgtcgccgcg
    73201 cgatgcaatc taccaggctt gtctgttgcg ttttcgtccg atcctgatga ccaccctggc
    73261 ggctctgctt ggcgcgctgc cgctgatgtt gagtaccggg gtcggcgcgg aactgcgtcg
    73321 tccgttaggt atcggcatgg tcggcggtct gattgtcagc caggtgctga cgctgttcac
    73381 cacgccggtg atttatctgc tgttcgaccg cctggcattg tggaccaaaa gtcgctttgc
    73441 tcgccatgaa gaggaggcgt aagtgaagtt ttttgccctc ttcatttacc gcccggtggc
    73501 gacgatttta ctgtcggtcg ccattaccct gtgcggcata ctgggcttcc gtatgctgcc
    73561 ggtcgccccg ctgccgcagg tcgattttcc ggtgattatg gtcagcgcct cgctgcccgg
    73621 tgcgtcacca gaaacaatgg cgtcttccgt tgccacgccg ctggagcgct cgcttgggcg
    73681 cattgccggg gtcagcgaaa tgacctccag cagttcgctc ggcagcacac gtattatttt
    73741 acagtttgat tttgaccggg acatcaacgg cgcagcgcgt gatgtgcagg cggcgatcaa
    73801 cgctgcacaa agtttgctgc ccagtgggat gcccagccgt ccgacctatc gcaaagcgaa
    73861 cccgtcggat gcgccaatta tgatcctcac gctgacatcc gatacttatt cgcagggtga
    73921 actgtacgat ttcgcctcga cgcagctggc tccgacgatt tcgcaaatcg acggtgttgg
    73981 tgatgtcgat gtcggaggca gctcactgcc cgccgtacgc gtcgggctga atccgcaggc
    74041 gctgtttaat cagggcgtgt cgctggacga cgtacgcacc gccatcagca atgccaacgt
    74101 gcgtaaaccg cagggcgcgc tggaagatgg cactcaccgc tggcagatcc agaccaatga
    74161 tgagctaaaa accgccgctg aatatcagcc gttgattatt cactacaaca acggcggcgc
    74221 ggttcgtctg ggcgatgtgg cgacggtgac cgactcagtg caggatgtgc gcaacgccgg
    74281 gatgaccaac gccaaaccgg ctattttact gatgatccgc aaactgccgg aagccaatat
    74341 tatccagacg gttgacagca tccgggcaaa attaccggag ttgcaggaaa ccattccggc
    74401 ggcgattgat ctgcaaattg cccaggatcg ctcccccacc attcgcgcct cgctggaaga
    74461 agtcgagcaa acgctgatta tctcggtggc gctggtgatt ctggtggtgt ttttattcct
    74521 gcgctcgggt cgcgccacta ttattcccgc cgtttcggtg ccggtttcgc tgattggtac
    74581 gtttgcggcg atgtacctgt gcggattcag tctcaataac ctttcgttaa tggcgctcac
    74641 catcgctact ggtttcgtgg tggatgacgc catcgtggtg ctggaaaaca ttgcacgtca
    74701 tctggaagcg ggaatgaaac cgttgcaagc cgcactgcaa ggtactcgcg aagtcggttt
    74761 tacggtgctg tcgatgagtc tgtcactggt ggcggtgttc ctgccgctgc tgttgatggg
    74821 cggattgccg ggccgactgt tacgcgagtt tgccgtgacg ctttctgtcg ccattggtat
    74881 ttcgttgctg gtttctctga cattaacgcc aatgatgtgt ggctggatgc tgaaagccag
    74941 caagccgcgc gagcaaaagc gactgcgtgg ttttggtcgc atgttggtag ccctgcaaca
    75001 aggctacggc aagtcactga aatgggtgct caatcatacc cgtctggtgg gcgtggtgct
    75061 gcttggcacc attgcgctga atatctggct gtatatctcg atcccgaaaa ccttcttccc
    75121 ggagcaggac actggcgtgt tgatgggcgg gattcaggcg gatcagagta tttcgtttca
    75181 ggcgatgcgc ggtaagttgc aggatttcat gaaaattatc cgtgacgatc cggcagtgga
    75241 taatgtcacc ggctttacag gcggttcgcg agtgaacagc gggatgatgt ttatcaccct
    75301 caagccacgc gacgaacgca gcgaaacggc gcagcaaatt atcgaccgtc tgcgcgtaaa
    75361 actggcgaaa gaaccggggg cgaatctgtt cctgatggcg gtacaggata ttcgcgttgg
    75421 tgggcgtcag tcgaacgcca gctaccagta cacgttgtta tccgacgacc tggcggcact
    75481 gcgcgaatgg gagccgaaaa tccgcaaaaa actggcgacg ttgccggaac tggcggacgt
    75541 gaactccgat cagcaggata acggcgcgga gatgaatctg gtttacgacc gcgacaccat
    75601 ggcacggctg ggaatcgacg tgcaggccgc caacagcctg ttaaataacg ccttcggtca
    75661 gcggcaaatc tcgaccattt accagccgat gaaccagtat aaagtggtga tggaagtgga
    75721 tccgcgctat acccaggaca tcagtgcgct ggaaaaaatg ttcgttatca ataacgaagg
    75781 caaagcgatc ccgctgtcgt atttcgctaa atggcaaccg gcgaacgctc cgctgtcggt
    75841 gaatcatcag ggattatcgg cggcctcgac catctcgttc aacctgccga ccggaaaatc
    75901 gctctcggac gccagtgcgg cgatcgatcg cgcaatgacc cagcttggtg tgccttcgac
    75961 ggtgcgcggc agttttgccg gaacagcgca ggtgttccag gagacgatga actcacaggt
    76021 gatcctgatt atcgccgcca tcgccacggt gtatatcgtg ctgggtatcc tttacgagag
    76081 ttacgtacac ccactgacga ttctctccac cctgccctcg gcgggcgttg gtgcgctgct
    76141 ggcgctggag ctgttcaatg ccccgttcag cctaatagcc ctgataggga tcatgctatt
    76201 aatcggcatc gtaaagaaaa acgccattat gatggtcgat tttgcgctcg aagcccaacg
    76261 gcacggtaac ctgacgccgc aggaagctat tttccaggcc tgtctgctgc gttttcgccc
    76321 gattatgatg actaccctgg cggcgctgtt tggcgcgctg ccgctggtgt tgtcgggcgg
    76381 tgacggctcg gagctgcggc acctcctggg gatcaccatt gtcggtggac tggtaatgag
    76441 ccagcttctg acgctgtata ccacgccggt ggtgtatctc tttttcgacc gtctgcggct
    76501 gcgtttttcg cgtaaaccta aacaaacggt aaccgagtaa atgacagaac ttcccgacag
    76561 cacccgctgg caattgtgga ttgtggcttt cggcttcttt atgcagtcgc tggacaccac
    76621 catcgttaac accgcccttc cctcaatggc gcaaagcctc ggggaaagtc cgttgcatat
    76681 gcacatggtc attgtctctt atgtgctgac cgtggcggtg atgctgcccg ccagcggctg
    76741 gctggcggac aaagtcggcg tgcgcaatat tttctttacc gccatcgtgc tgtttactct
    76801 cggttcactg ttttgcgcgc tttccggcac gctgaacgaa ctgttgctgg cacgcgcgtt
    76861 acagggcgtt ggcggtgcga tgatggtgcc ggtcggcagg ttgacggtga tgaaaatcgt
    76921 accgcgcgag caatatatgg cggcgatgac ctttgtcacg ttacccggtc aggtcggtcc
    76981 gctgctcggt ccggcgctcg gcggtctgct ggtggagtac gcgtcgtggc actggatctt
    77041 tttgatcaac attccggtgg ggattatcgg tgcgatcgcc acattgctgt taatgccgaa
    77101 ctacaccatg cagacgcggc gctttgatct ttccggcttt ttattgctgg cggttggcat
    77161 ggctgtatta accctggcgc tggacggcag taaaggtaca ggtttatcgc cgctggcgat
    77221 tgctggcctg gtcgcagttg gcgtggtggc actggtgctt tatctgctgc acgccagaaa
    77281 taacaaccgc gccctgttca gtctgaaact gttccgcact cgtacctttt cgctgggcct
    77341 ggcggggagc tttgccggac gtattggcag tggcatgttg ccctttatga caccggtttt
    77401 cctgcaaatt ggcctcggtt tctcgccgtt tcatgccgga ctgatgatga tcccgatggt
    77461 acttggcagc atgggaatga agcgaattgt ggttcaggtg gtgaatcgct ttggttatcg
    77521 tcgggtactg gtagcgacca cgctgggtct gtcgctgatc accctgttgt ttatgactac
    77581 cgctctgttg ggctggtact acgttttgcc gttcgtcctg tttttacaag ggatggtcaa
    77641 ctcgacccgt ttctcctcca tgaacaccct gacgctgaaa gatctcccgg acaatctggc
    77701 gagcagcggc aacagtctgc tgtcgatgat tatgcaattg tcgatgagta tcggcgtcac
    77761 catcgccggg ctgttgctgg gactttttgg ttcgcagcat gtcagcgtcg acagcggcac
    77821 cacacaaacc gtctttatgt acacctggct tagcatggcg tttatcatcg cccttccggc
    77881 gttcatcttt gccagagtgc cgaacgatac gcatcaaaat gtagctattt cgcggcgaaa
    77941 aaggagcgcg caatgaagtt ctggcgacct ggtattaccg gcaaactgtt tctggcgatt
    78001 ttcgccacct gcattgtttt gctgatcagt atgcactggg cggtgcgtat cagttttgag
    78061 cgtggcttta ttgattacat caagcatggt aatgaacagc gattacaact gttaagtgat
    78121 gcgcttggcg agcagtatgc gcagcatggc aactggcgct tcctgcgcaa caatgatcgc
    78181 tttgtctttc agatcctgcg ttcatttgaa cacgataatt cggaagataa acccggcccg
    78241 ggtatgccac cgcacggctg gcgtacccag ttctgggtgg ttgatcaaaa caacaaagtg
    78301 ctggttggtc cgcgagcgcc gattccacct gacggtacac ggcgacccat tctggtcaac
    78361 ggtgcggaag ttggcgcggt gatcgcctcc cccgttgagc ggttaacgcg caatactgat
    78421 atcaatttcg ataaacaaca gcggcaaacc agctggttga ttgtcgccct ggcaacgtta
    78481 ctcgcggcac ttgccacttt tctgctggcg cgcggtttac tggcaccggt aaaacgactt
    78541 gtcgatggca cgcacaaact ggcggcgggc gatttcacta cccgcgtaac gcccaccagt
    78601 gaagatgaac tgggcaaact ggcgcaagac ttcaaccagc ttgccagcac actggagaaa
    78661 aaccagcaaa tgcggcgcga ttttatggcc gatatttctc acgaactgcg tacgccatta
    78721 gcggtgctgc gcggtgaact ggaagctatt caggatggcg tgcgcaaatt cacgccggag
    78781 acggtggcgt ctttacaggc ggaggtcggt acactgacca aactggttga cgatctccat
    78841 cagttgtcga tgtctgatga aggcgccctc gcctatcaaa aagcaccggt agatttgatc
    78901 ccactgctgg aagtggcggg cggcgcattt cgcgaacgat tcgccagccg tggcctgaaa
    78961 ctgcaatttt ccctgccaga cagtattacc gtatttggcg atcgcgaccg tttaatgcag
    79021 ttattcaata atttacttga aaacagcctg cgctacactg acagcggcgg cagcctgaaa
    79081 atctctgccg agcagcacga caaaacggtg cgcctgacct ttgccgacag tgcgccaggt
    79141 gtcagtgacg agcagctaca aaaattattt gaacgttttt atcgcaccga aggttcccgc
    79201 aaccgcgcca gcggtggttc cgggctgggg ctggcgattt gcctgaacat tgttgaagca
    79261 cataatggcc gcattattgc tgcccattcg ccttttggcg gggtaagcat tacagtagag
    79321 ttaccgctgg aacgggattt acagagagaa gtatgaccga gttaccaatc gacgaaaaca
    79381 caccgcgtat tttgatcgtg gaagatgaac cgaagctggg gcagttgctc attgattatc
    79441 tgcgtgctgc gagctatgcg ccgacgctta tcagccacgg cgatcaggta ctgccgtatg
    79501 tgcgccagac accaccggat ctgatcctgt tggatctgat gctccctggc accgatggct
    79561 tgacgctgtg ccgggaaatt cgtcgttttt ctgacattcc tatcgtgatg gtgacggcaa
    79621 aaatcgaaga gatcgaccgc ctgctcggac tggagattgg cgcagatgac tacatctgca
    79681 agccgtacag cccacgggaa gtggtagcgc gcgtcaaaac cattttgcgc cgttgcaaac
    79741 cgcagcgcga attgcagcaa caggatgccg aaagcccgtt gattatcgac gaaggtcgtt
    79801 ttcaggcttc atggcgcggt aaaatgcttg acctgacgcc tgcggaattt cgtctgttga
    79861 aaacgctctc tcatgaacca ggaaaagtgt tctcccgcga gcaattgctc aatcatcttt
    79921 atgacgacta ccgcgtagta accgaccgca ccattgacag ccacattaaa aacctgcgcc
    79981 gcaagctgga atctctcgac gccgaacagt catttatccg cgccgtttac ggcgttggtt
    80041 accgctggga agccgacgcc tgccgcatcg tttagtttta gcgacatgac tttgttagcc
    80101 ggagatggcg ttcagaccag atccggcaac attatcccac gcatggtcag caaactga
//
LOCUS       DAZPJR010000003        64592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPJR010000003 DAZPJR010000000
VERSION     DAZPJR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.004"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHVB7_000206"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHVB7_000206"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR1912317.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHVB7_000207"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHVB7_000207"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR1912318.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHVB7_000208"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHVB7_000208"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR1912319.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHVB7_000209"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHVB7_000209"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR1912320.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHVB7_000210"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHVB7_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR1912321.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHVB7_000211"
     CDS             complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHVB7_000211"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR1912322.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHVB7_000212"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHVB7_000212"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR1912323.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLCLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="ACHVB7_000213"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="ACHVB7_000213"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR1912324.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHVB7_000214"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHVB7_000214"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR1912325.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="ACHVB7_000215"
     CDS             complement(12799..14139)
                     /locus_tag="ACHVB7_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR1912326.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHVB7_000216"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHVB7_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR1912327.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="ACHVB7_000217"
     CDS             complement(15928..16377)
                     /locus_tag="ACHVB7_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR1912328.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHVB7_000218"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHVB7_000218"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR1912329.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="ACHVB7_000219"
     CDS             complement(17177..17506)
                     /locus_tag="ACHVB7_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR1912330.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHVB7_000220"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHVB7_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR1912331.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="ACHVB7_000221"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="ACHVB7_000221"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR1912332.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHVB7_000222"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHVB7_000222"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR1912333.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21621..22910
                     /gene="sdaC"
                     /locus_tag="ACHVB7_000223"
     CDS             21621..22910
                     /gene="sdaC"
                     /locus_tag="ACHVB7_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR1912334.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22968..24335
                     /gene="sdaB"
                     /locus_tag="ACHVB7_000224"
     CDS             22968..24335
                     /gene="sdaB"
                     /locus_tag="ACHVB7_000224"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR1912335.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNASRMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24447..25202
                     /gene="xni"
                     /locus_tag="ACHVB7_000225"
     CDS             24447..25202
                     /gene="xni"
                     /locus_tag="ACHVB7_000225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR1912336.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="ACHVB7_000226"
     CDS             complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="ACHVB7_000226"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR1912337.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="ACHVB7_000227"
     CDS             complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="ACHVB7_000227"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR1912338.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27677..28993
                     /gene="fucP"
                     /locus_tag="ACHVB7_000228"
     CDS             27677..28993
                     /gene="fucP"
                     /locus_tag="ACHVB7_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR1912339.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29026..30801
                     /gene="fucI"
                     /locus_tag="ACHVB7_000229"
                     /pseudo
     CDS             29026..30801
                     /gene="fucI"
                     /locus_tag="ACHVB7_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            30910..32328
                     /gene="fucK"
                     /locus_tag="ACHVB7_000230"
     CDS             30910..32328
                     /gene="fucK"
                     /locus_tag="ACHVB7_000230"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR1912340.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32330..32752
                     /gene="fucU"
                     /locus_tag="ACHVB7_000231"
     CDS             32330..32752
                     /gene="fucU"
                     /locus_tag="ACHVB7_000231"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR1912341.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32810..33541
                     /gene="fucR"
                     /locus_tag="ACHVB7_000232"
     CDS             32810..33541
                     /gene="fucR"
                     /locus_tag="ACHVB7_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR1912342.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="ACHVB7_000233"
     CDS             complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="ACHVB7_000233"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR1912343.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34678..35073)
                     /locus_tag="ACHVB7_000234"
     CDS             complement(34678..35073)
                     /locus_tag="ACHVB7_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR1912344.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="ACHVB7_000235"
     CDS             complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="ACHVB7_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR1912345.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36360..36587)
                     /locus_tag="ACHVB7_000236"
     CDS             complement(36360..36587)
                     /locus_tag="ACHVB7_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR1912346.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36779..37984
                     /gene="csdA"
                     /locus_tag="ACHVB7_000237"
     CDS             36779..37984
                     /gene="csdA"
                     /locus_tag="ACHVB7_000237"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR1912347.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37984..38427
                     /gene="csdE"
                     /locus_tag="ACHVB7_000238"
     CDS             37984..38427
                     /gene="csdE"
                     /locus_tag="ACHVB7_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR1912348.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="ACHVB7_000239"
     CDS             complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="ACHVB7_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR1912349.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="ACHVB7_000240"
     CDS             complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="ACHVB7_000240"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR1912350.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40667..40743
                     /locus_tag="ACHVB7_000241"
     tRNA            40667..40743
                     /locus_tag="ACHVB7_000241"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40701..40703,aa:Met,seq:cat)
     gene            complement(40818..42071)
                     /gene="amiC"
                     /locus_tag="ACHVB7_000242"
     CDS             complement(40818..42071)
                     /gene="amiC"
                     /locus_tag="ACHVB7_000242"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR1912351.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42303..43634
                     /gene="argA"
                     /locus_tag="ACHVB7_000243"
     CDS             42303..43634
                     /gene="argA"
                     /locus_tag="ACHVB7_000243"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR1912352.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43696..45522)
                     /gene="recD"
                     /locus_tag="ACHVB7_000244"
     CDS             complement(43696..45522)
                     /gene="recD"
                     /locus_tag="ACHVB7_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR1912353.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKRRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45522..49064)
                     /gene="recB"
                     /locus_tag="ACHVB7_000245"
     CDS             complement(45522..49064)
                     /gene="recB"
                     /locus_tag="ACHVB7_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1912354.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49057..51945)
                     /gene="ptrA"
                     /locus_tag="ACHVB7_000246"
     CDS             complement(49057..51945)
                     /gene="ptrA"
                     /locus_tag="ACHVB7_000246"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR1912355.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52121..55489)
                     /gene="recC"
                     /locus_tag="ACHVB7_000247"
     CDS             complement(52121..55489)
                     /gene="recC"
                     /locus_tag="ACHVB7_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR1912356.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55502..55825)
                     /locus_tag="ACHVB7_000248"
     CDS             complement(55502..55825)
                     /locus_tag="ACHVB7_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR1912357.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55810..56217)
                     /locus_tag="ACHVB7_000249"
     CDS             complement(55810..56217)
                     /locus_tag="ACHVB7_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR1912358.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56214..56777)
                     /locus_tag="ACHVB7_000250"
     CDS             complement(56214..56777)
                     /locus_tag="ACHVB7_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1912359.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56768..57238)
                     /locus_tag="ACHVB7_000251"
     CDS             complement(56768..57238)
                     /locus_tag="ACHVB7_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1912360.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57423..58217)
                     /gene="thyA"
                     /locus_tag="ACHVB7_000252"
     CDS             complement(57423..58217)
                     /gene="thyA"
                     /locus_tag="ACHVB7_000252"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1912361.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58224..59099)
                     /gene="lgt"
                     /locus_tag="ACHVB7_000253"
     CDS             complement(58224..59099)
                     /gene="lgt"
                     /locus_tag="ACHVB7_000253"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR1912362.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59250..61496)
                     /gene="ptsP"
                     /locus_tag="ACHVB7_000254"
     CDS             complement(59250..61496)
                     /gene="ptsP"
                     /locus_tag="ACHVB7_000254"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1912363.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61509..62039)
                     /gene="rppH"
                     /locus_tag="ACHVB7_000255"
     CDS             complement(61509..62039)
                     /gene="rppH"
                     /locus_tag="ACHVB7_000255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR1912364.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(62392..62538)
                     /gene="ygdT"
                     /locus_tag="ACHVB7_000256"
     CDS             complement(62392..62538)
                     /gene="ygdT"
                     /locus_tag="ACHVB7_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR1912365.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            62724..63413
                     /gene="mutH"
                     /locus_tag="ACHVB7_000257"
     CDS             62724..63413
                     /gene="mutH"
                     /locus_tag="ACHVB7_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR1912366.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63482..64195
                     /locus_tag="ACHVB7_000258"
     CDS             63482..64195
                     /locus_tag="ACHVB7_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1912367.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64333..64551
                     /gene="ygdR"
                     /locus_tag="ACHVB7_000259"
     CDS             64333..64551
                     /gene="ygdR"
                     /locus_tag="ACHVB7_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR1912368.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15652 a        17368 c        15824 g        15748 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cttggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 tcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc acaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaagcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gagaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgtgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagggtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    18961 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggac atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgcgttgc
    19981 aggccaactt gttcgcggtt ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcactgcatg tcggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggaca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaatcagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcacagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgttcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tgcatacgtt gggtgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    20761 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacatacc
    21121 catcaattta tttcatttgt tatatgaatg tttcttacca ccctcacgcg ataaatatca
    21181 ttgcagttaa tatgtcatac aatttatgtt gtaacgcaaa cgtttcccta ttttcataaa
    21241 ccacatttta tcgctatgaa aaagaaatta tcgccatgat taactaaaag tattgatttt
    21301 ttcagttcaa cctacatata ttgcgcgccc cggaagaagt cagatgtcgt ttaatgggca
    21361 aatattgccc ttaaattctc ttttactttt gatttacaga gtaaagcgtt gggataatct
    21421 atcttccaag tagattattg tatttgggat caagatcact gatagataca taacttgtgt
    21481 gtatctttcc gccctcaaat tattacggcg gtaaatgatt aagccatcgc cgatagacag
    21541 atttcatttt tacggtcagg caccttcccg agctgaactg gctaaaagct gaattatttg
    21601 cattcctcca ggagaaatag atggaaacga ctcaaaccag cacgattgcg tcgaaagact
    21661 ctcgtagtgc ctggcgcaag acagacacca tgtggatgct gggcctttac ggcacggcaa
    21721 tcggcgcggg cgtgctgttc ctgccaatca acgccggtgt tggcggtatg atcccgctga
    21781 tcatcatggc tatccttgcg ttcccgatga cattttttgc tcaccgcggc ctgactcgct
    21841 tcgtactgtc tggtaaaaac ccaggcgaag acatcaccga ggttgtagaa gaacactttg
    21901 gtattggcgc aggtaaactg attaccctgc tctacttctt cgctatctac ccgatcctgc
    21961 tggtttatag cgtggcaatc accaatacag ttgaaagctt catgtctcac cagctgggta
    22021 tgacaccacc gccgcgtgcg attctgtcgc tgatcctgat cgtgggtatg atgaccatcg
    22081 ttcgcttcgg tgagcagatg atcgttaaag caatgagtat tctggtattc ccgtttgttg
    22141 gcgtactgat gctgctggct ctgtacctga tcccgcagtg gaacggcgca gcactggaaa
    22201 cgctgtctct ggacactgca tctgcaaccg gaaacggtct gtggatgacc ctgtggctgg
    22261 caattccggt tatggtgttc tctttcaacc actctccgat catctcttct ttcgccgttg
    22321 cgaagcgtga agagtacggc gatatggcag aacagaaatg ctcgaagatc ctggcattcg
    22381 cacacatcat gatggtgctg accgtaatgt tcttcgtctt cagctgtgta ctgagcctga
    22441 ctccggcaga cctggctgcg gctaaagagc agaacatctc gattctgtct tacctggcta
    22501 accactttaa cgcaccggtt atcgcgtgga tggctccgat tatcgcgatt atcgctatca
    22561 ccaaatcctt cctcggccac tacctgggcg cacgtgaagg cttcaacggt atggtgatta
    22621 aatctctgcg tggtaaaggt aagtctatcg aaatcaacaa gctgaaccgt atcactgcgc
    22681 tgttcatgct ggtaacgacc tggattgttg ccaccctgaa cccgagcatc ctgggtatga
    22741 ttgaaaccct gggcggccca atcatcgcga tgatcctgtt cctgatgccg atgtacgcaa
    22801 ttcagaaagt accggcaatg cgtaagtaca gcggtcacat cagcaacgta ttcgttgtcg
    22861 tgatgggtct gattgcaatc tccgcaatct tctactctct gttcagctaa gtcctttcgc
    22921 gccgctttcg ggcggcgctt cctccgtttt aacacgatgt atttcctatg attagcgtat
    22981 tcgatatttt caaaatcggc attggccctt ccagttctca taccgttgga ccaatgaaag
    23041 cgggtaaaca atttaccgac gatctgattg cccgtaatct gcttaaagac gtgacccgcg
    23101 tggtggttga cgtgtacggc tcgctctctc tgaccggtaa aggccaccac actgatatcg
    23161 ccattattat gggcctggcg ggtaacctgc cggataccgt ggatatcgat tccatccctg
    23221 gttttattca ggatgtgaat actcatggtc gcctgatgct ggcaaacggt cagcatgaag
    23281 tggagttccc ggttgatcag tgcatgaact tccatgcaga caacctttct ctgcatgaaa
    23341 acggtatgcg cattaccgcg ctggcgggcg ataaagtcgt ttacagccag acttactact
    23401 ctattggcgg tggctttatc gttgatgaag agcattttgg ccagcagaat agcgcaccgg
    23461 ttgaagttcc ttatccgtac agttcagcag ccgatctgca aaaacattgt caggaaaccg
    23521 ggctgtcact ctctggcctg atgatgaaaa atgagctggc gctgcacagc aaagaagagc
    23581 tggaacagca cctggcgaac gtatgggaag tcatgcgtgg cggtattgag cgcggtattt
    23641 ccaccgaagg cgtgttgcct ggcaaactgc gcgttccacg ccgtgctgcg gcactacgcc
    23701 ggatgctggt cagccaggat aaaaccacca ctgacccgat ggcggttgtt gactggatca
    23761 acatgtttgc actggcagtg aacgaagaga acgctgctgg cggacgcgtg gtgactgcgc
    23821 cgactaacgg tgcgtgcggg attatcccgg cagtgctggc gtactacgac aagttcatcc
    23881 gcgaagtgaa cgctaactca ctggctcgtt acctgctggt agccagtgcc attggttctc
    23941 tttataagat gaacgcgtca atttctggtg ctgaagtcgg ctgccagggt gaagttggtg
    24001 tggcgtgctc aatggcggcg gctggtctgg cagaactgtt aggtgcaagc ccggcgcagg
    24061 tgtgcatcgc ggcggaaatc gccatggagc acaacctcgg tctgacgtgt gacccggtcg
    24121 ccggacaggt ccaggtgcca tgcatcgagc gtaacgccat tgcggcagta aaagcggtga
    24181 acgcctcacg tatggcgcta cgccgtacca gcgagccgcg cgtctgcctc gataaagtta
    24241 tcgaaaccat gtacgaaaca ggtaaagata tgaacgccaa gtaccgcgaa acctctcgcg
    24301 gcggcctggc aatgaagatc gttgcctgcg attaatcgct ctccagaggc ctcgttttgc
    24361 gaggcctctt cccgatttct catccagccg tagcctgttc cggcatcgaa tgttaccctt
    24421 atcgcctgat ctttaagggg gttatcgtgg ctgttcattt gcttattgtc gatgcactga
    24481 atcttattcg tcgcattcat gccgttcagg gatcgccctg tgtcgaaacc tgccagcatg
    24541 cgctcgatca gctcattatg cacagccagc caacccacgc ggtcgccgtt tttgatgatg
    24601 aaaaccgcag tagcggctgg cgtcatcagc gtttaccaga ttacaaagcg ggtcgaccgc
    24661 caatgccgga agagttgcac gacgagatgc ctgcattacg cgccgccttt gagcaacgcg
    24721 gcgtcccgtg ctggtcaacc agcggcaacg aagccgatga cttagccgcc acgctggcgg
    24781 tcaaagtgac acaggccggg catcaggcaa cgattgtttc gacagataaa ggctactgtc
    24841 agttactttc accgacatta cgtattcgtg attacttcca gaaacgttgg ctggatgcgc
    24901 catttatcga taaagaattt ggcgttcaac cgcagcagtt gcccgattac tggggacttg
    24961 cggggatcag cagttcaaag gtaccgggtg ttgcgggaat cggaccaaaa agcgccacgc
    25021 agctgctggt cgagtttcag agtctggaag ggatatatga gaatctggat gcggttgccg
    25081 aaaagtggcg caaaaaatta gaaacccata aagagatggc gtttctgtgc cgcgatattg
    25141 cccgcttaca aaccgatttg catatcgacg gcaatttaca gcaattgcgg ttggtacggt
    25201 aacggcgagc cggatgcaaa cgattatcaa cacattgaat ttaatgcact tatataccgg
    25261 ataagacgcg gctagcgtcg tatccggcat tatcacatca gcgcatttac caggcggtat
    25321 ggtaaagctc tacaatatcc tcaagcgttg cttcacgcgg gttgccaccg gtacaaacat
    25381 catccagtgc cgcctgcgcc agtgccggaa tgtcttcctt gcgtacacca acatcacgca
    25441 aatgtggcgg aataccgaca tcacggttga gagcaaacac cgcttcaaca gcggcattac
    25501 gcgcctcttc caggctcata ccttccactt tcacgcccat aacgcgcgcg atatcgcggt
    25561 acttctcacc ggtaaagtca gcgttatagt gcatgacatg cggtaacagg atggcgttcg
    25621 caacaccgtg tggagtgttg taaaacgcgc ccagtggatg cgccatacca tgcaccaacc
    25681 ctaacccaac attcgagaag cccatacccg caacatactg cccgagcgcc atttcttctc
    25741 cggcatcctt atcaccagca accgatcctc gcagcgcccc agcaatgatt tcaatcgctt
    25801 taatgtgcag tgcatcggtt agcgcccacg cgccacgggt aatatacccc tcaatagcat
    25861 gagtgagcgc atcgacaccc gtcgcagctt tcagcgctgg aggcatacca tccatcatgt
    25921 cagcgtcaat aaacgccacc tgcgggatat catgcggatc aacgcaaaca aacttgcgcc
    25981 gtttctcttc gtcagtgatc acgtagttaa tggtcacttc tgccgcagta cctgctgtgg
    26041 taggaattgc cagaatcggt acactgggtt tattggtcgg ggaaagccct tccaggctac
    26101 gcacatcggc aaactccgga ttgttgctga taatgccaat cgctttacaa gtatcctgtg
    26161 gagaaccacc accaatagcg atcaggtaat ccgcgccgct attctggaat acaccgagcc
    26221 cttctttgac gacagtaatt gttgggttgg gcactacgcc gtcgtaaatc gcccatgcca
    26281 gccctgcagc atccatctta tcggtcactt tcgccaccac gccgcattgc accagcgttt
    26341 tatcggtgac gatcagcgcc ttctgataac cacggcgttt cacctcatcg gttaaagccc
    26401 caacagcacc ccgaccaaac catgccgttt cgttcagaat cattctgtta gccatcatcc
    26461 ttctccttgt tgctttacga aattactctt caatccgtaa cccataggtt ttgaatttct
    26521 ccagcactac ggcaatctct tcatcgctca gcactggcac cgggtccgta atcgccaggg
    26581 tcgccacgta aagttgcgcc agcacttcaa cttcatgcgc cagccataac gctttttcca
    26641 gattcacctc acaagcgata agcccatgat gttgtaacaa agttgcctta cgatttttga
    26701 gagccagcgc aacatgttca gaaagttcgc gtgttccaaa ggtcgcataa ggcgcgcaag
    26761 gaatagaatt accacctgcc gccgcaatca tgtagtgaat agcggggatc ggtcggttaa
    26821 gaatggaaac tgctgtgcaa tgaacggcat gattgtgaac aaccgcgttg gcatccggtc
    26881 tgctttgata ggccgccata tggaaacgcc attcgcttga ggggagcttt ccttcctcat
    26941 gtttaccgtt gccatcaata aagacaatat gcgactccgt cagtttttca tatggaatgc
    27001 ctgtaggcgt aatcagcatc ccatcctgat aacgtacact gacgttcccc gctgtcccct
    27061 ggttcagtcc caggcgggtc atttccaggc aagtgtcaat aatctgacga gcaagtttat
    27121 ttcgttccat tagctacctc tctctgattc aaaacagggc aataatgttg ttcctttcac
    27181 actattgaat tagccgttta attacccacc atcttcttcc tgattaacaa gaaagaaatt
    27241 cacaagctta tattttgtga cctagctcaa ctaatcacag taaataactg caagttctct
    27301 ttttataacc ccattaaaaa tgaccgctct taaaaatatt tatcaaaacg gtcatttttc
    27361 tattcaccca agccaggaat gcccgttttc ggcataaaca attaatacag tcatctggtt
    27421 tgtgtttttt atgatttatt ttctgaaacg ggcatgaaat ttcgattatt aaagtgatgg
    27481 tagtcacata aagtcacctt ccagctaata agtgtgaccg ccgtcatatt acagagcgtt
    27541 ttttatttga aaatgaatcc atgagttcat ttcagacagg caaatattca ctgatatgaa
    27601 gcccgaactc gctggttttg cacttttgaa aacataaccg attacgtgct taagcttctg
    27661 aacctaagag gatgctatgg gaaacacatc aatacaaacg cagagttacc gtgcggtaga
    27721 taaagatgca gggcaaagca gaagttacat tattccattc gcgctgctgt gctcactgtt
    27781 ttttctttgg gcggtagcca ataaccttaa cgacatttta ttacctcaat tccagcaggc
    27841 ttttacgctg acaaatttcc aggctggcct gatccaatcg gccttttact ttggttattt
    27901 cattatccca attcctgctg ggatattgat gaaaaaactc agttataaag caggaattat
    27961 tactgggtta tttttatatg ccctgggcgc tgcattattc tggcccgctg cagaaataat
    28021 gaactacacc ttatttttag ttggcctatt tattattgca gccggattag gttgtctgga
    28081 aactgccgca aacccttttg ttacggtatt agggccggaa agcagtggtc acttccgctt
    28141 aaatcttgcg caaacattta actcgtttgg cgcaattatc gcggttgtct ttgggcaaag
    28201 acttattttg tctaacgtgc cacatcaatc gcaagacgtt ctcgataaaa tgtctccaga
    28261 gcaattgagt gcgtataaac acagcctggt attatcggta cagacacctt atatgatcat
    28321 cgtggctatc gtgttactgg tcgccctgct gatcatgctg acgaaattcc cggcattgca
    28381 gagtgataat cacagtgacg ccaaacaagg atcgttctcc gcatcgcttt ctcgcctagc
    28441 gcgtattcgc cactggcgct gggcagtatt agcgcaattc tgctacgttg gcgcacaaac
    28501 ggcctgctgg agctatttga ttcgctacgc tgtagaagaa attccaggta tgactgcagg
    28561 ctttgccgct aactatttaa ccggaaccat ggtgtgcttc tttattggtc gtttcaccgg
    28621 tacctggctc atcagtcgct tcgcaccaca caaagtcctg gccgcctacg cattaatcgc
    28681 tatggcactg tgcctgatct cagccttcgc tggcggtcat gtgggcttaa tagccctgac
    28741 tttatgcagc gcctttatgt cgattcagta cccaacaatc ttctcgctgg gcattaagaa
    28801 tctcggccag gacaccaaat acggttcgtc cttcatcgtt atgaccatta ttggcggcgg
    28861 tattgtcact ccggtcatgg gttttgtcag tgacgcggcg ggcaacatcc ccactgctga
    28921 actgatcccc acactctgct tcgcggtcat ctttatcttt gcccgtttcc gctctcaaac
    28981 ggcaactaac tgaacatatt ttccgaataa agtgaggaat ctgtaatgaa aaaaatcagc
    29041 ttaccgaaaa ttggtatccg cccggttatt gacggtcgtc gcatgggtgt tcgtgagtcg
    29101 cttgaagaac aaacaatgaa tatggcgaaa gctacggccg cactgctgac cgagaaactg
    29161 cgccatgcct gcggagctgc cgtcgagtgt gtcatttccg atacctgtat cgcgggtatg
    29221 gctgaagccg ctgcttgcga agaaaaattc agcagctaga atgtaggcct caccattacg
    29281 gtaacgcctt gctggtgcta tggcagtgaa accatcgaca tggatccaac ccgcccgaaa
    29341 gccatttggg gctttaacgg cactgaacgt cccggcgctg tttacctggc agcggctctg
    29401 gcagctcaca gccagaaagg catcccggca ttctccattt acggtcatga cgttcaggat
    29461 gccgatgaca catcgattcc tgccgatgtt gaagaaaaac tgctgcgctt tgcccgcgcc
    29521 ggtttggccg tcgccagcat gaaaggtaaa agctatctgt cgctgggcgg cgtttcgatg
    29581 ggtatcgccg gttctattgt tgatcacaac ttctttgaat cctggctggg aatgaaagtc
    29641 caggcggtgg atatgaccga actgcgtcgc cgtatcgatc agaagattta cgacgaagcc
    29701 gaactggaaa tggcactgac ctgggctgat aaaaacttcc gctatggcga agatgaaaat
    29761 aacaaacagt atcaacgcaa tgccgagcaa agccgcgcag ttctgcgcga aagtttactg
    29821 atggcgatgt gtatccgcga catgatgcaa ggcaacagca aactggccga tattggtcgc
    29881 gtggaagaat cacttggcta caacgccatc gctgcgggct tccaggggca acgtcactgg
    29941 accgatcaat atcccaatgg tgacaccgcc gaagcgatcc tcaacagttc atttgactgg
    30001 aatggcgtgc gcgaaccctt tgtcgtggcg accgaaaacg acagtcttaa cggcgtggca
    30061 atgctaatgg gtcaccagct caccggcacc gctcaggtat ttgccgatgt gcgtacctac
    30121 tggtcaccag aagcaattga gcgtgtaacg gggcataaac tcgatggact ggcagaacac
    30181 ggcatcatcc atttgatcaa ctcaggttct gctgcgctgg acggttcctg taaacaacgc
    30241 gacagcgaag gtaacccgac gatgaagcca cactgggaaa tctctcagca agaggctgac
    30301 gcttgcctcg ccgctaccga atggtgcccg gcgatccacg aatacttccg tggcggcggt
    30361 tactcttccc gcttccttac cgaaggcggc gtcccgttca ccatgactcg tgtcaacatc
    30421 atcaaaggcc tgggatcggt actgcaaatc gcggaaggct ggagcgtgga attgccgaag
    30481 gatgtgcatg acatcctcaa caaacgcacc aactcaacct ggccaaccac ctggtttgca
    30541 ccgcgcctca ccggtaaagg gccgtttacg gatgtgtact cggtaatggc gaactggggc
    30601 gctaaccatg gggttctgac catcggccac gttggcgcag actttatcac tctcgcctcc
    30661 atgctgcgta tcccggtatg tatgcacaac gttgaagaga ccaaagtgta tcgtccttct
    30721 gcctgggctg cgcacggcat ggatattgaa ggccaggatt accgcgcttg ccagaactac
    30781 ggtccgttgt acaagcgtta atacattact ccctgatgtg atgcccggtc gctccggcta
    30841 ccgtgcctga acaagcaaga gtggttagcc ggataagcaa tgttatccgg ctatattgca
    30901 ggagcgatta tgaaacaaga agttatcctg gtactcgact gtggcgcgac caatgtcagg
    30961 gccatcgcgg ttaatcggca gggaaaaatt gttgcccgcg cctcaacgcc taatgccagc
    31021 gatatcgcga tggaaaacaa cacctggcac cagtggtctt tagacgccat tttgcaacgc
    31081 tttgctgatt gctgtcggca aatcaatagt gaactgactg aatgccacat ccgcggtatc
    31141 gccgtcacca cctttggtgt ggatggcgct ctggtagata agcaaggcaa tctgctctat
    31201 ccgatcatta gctggaaatg tccgcgaaca gcagcggtta tggacaatat tgaacagttg
    31261 atctccgcac agcggttgca ggctatttct ggcgtcggag cctttagttt caatacgtta
    31321 tataagttgg tgtggttgaa agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg
    31381 ctctttattt cgtcgctgat taaccaccgt ttaaccggcg aattcactac tgatatcacg
    31441 atggccggaa ccagccagat gctggatatc cagcaacgcg atttcagtcc gcaaatttta
    31501 caagccaccg gtattccacg ccgactcttc cctcgtctgg tggaagcggg tgaacagatt
    31561 ggtacgctac agaacagcgc cgcagcaatg ctcggcttac ccgttggcat accggtgatt
    31621 tccgcaggtc acgataccca gttcgccctt tttggcgctg gtgccgacca aaatgaaccc
    31681 gtgctctctt ccggtacatg ggaaatttta atggtccgta gcgcccaggt tgatacttcg
    31741 ctgttaagtc agtacgccga ttctacctgc gaactggata gccaggcagg gttgtataac
    31801 ccaggtatgc aatggctggc atccggcgtg ctggaatggg tgagaaaact gttctggacg
    31861 gctgaaacac cctggcaaat gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat
    31921 ggtgtgaaaa tgcagtgtga tttattgtcg tgtcagaacg ctggctggca aggagtgacg
    31981 cttaatacca cgcgggggca tttctatcgc gcggcgctgg aagggttaac tgcgcaatta
    32041 cagcgcaatc tacagatgct ggaaaaaatc gggcacttta aagcctctga attattgtta
    32101 gtcggtggag gaagtcgcaa cacattgtgg aatcagatta aagccaatat gcttgatatt
    32161 ccggtaaaag ttctcgacga tgccgaaacg accgtcgcag gagctgcgct gttcggttgg
    32221 tatggcgtag gggaatttaa cagcccggaa gaagcccgtg cacagattca ttatcagtac
    32281 cgttatttct acccgcaaac tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac
    32341 aatttcgccg ttaatttctc ccgaactatt gaaagtgctg gcagagatgg gacatggaga
    32401 tgaaattatt ttttccgatg ctcactttcc cgcccattcg atgggaccgc aggtgatccg
    32461 cgctgatggc ctgttggtga gcgacttgct ccaagcgatt atcccattat ttgagctgga
    32521 cagttatgcc ccgccgctgg tgatgatggc agcggtagaa ggtgacactc tcgatcctga
    32581 agtagaacga cgttaccgta atgcgctttc actacaagcc ccgtgtcctg acatcatccg
    32641 catcaatcgt tttgcgtttt atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg
    32701 cgaacgagcg aagtacggga atattctttt aaaaaaaggg gtaacaccgt aatctcatat
    32761 cggtacgccc gcatgacgcg ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc
    32821 acgccagcaa gcgatagtcg acctgctgct gaaccatacc agcctgacca cggaagctct
    32881 ctctgaacag ctaaaggtca gtaaagaaac cattcgtcgc gatctcaatg aattacagac
    32941 gcagggtaaa attctgcgca atcatggacg cgctaaatat atccaccgtc aaaatcaaga
    33001 cagtggcgat ccctttcaca tcaggctgaa aagccattat gcgcataaag cagatatcgc
    33061 gcgcgaggcg ctcgcgtgga ttgaagaagg gatggtgata gccttagacg ccagttcaac
    33121 ttgctggtat ctggcacgcc agttgcctga catcaacatt caggtcttca ccaatagcca
    33181 tccgatttgc catgaactcg gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac
    33241 acttgagcgc aaatatggct gttacgtcaa tccctcgctg atttcccaac ttaaatcgct
    33301 ggaaatcgat ctgtttattt tttcttgtga agggatcgat agcagcggcg cactgtggga
    33361 ctccaatgcg atcaacgctg attacaaatc gatgctatta aaacgtgccg cgcaatcgtt
    33421 gttattgatt gataaaagta aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga
    33481 tgaggtaacg cacattattt ctgatgagcg ccaggttgca acttctttgg taacagcctg
    33541 acggaaaggg tagcaggccg aagacgacgc cccggccttg cctgttatcg ctcgtcgcga
    33601 cgaccaccca ccgcagccca gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga
    33661 tacaactgcc gcgcctgaat ctgagcattt atgccatgtt catcaagctg tgcctgaata
    33721 tacgccagat tgtgtgatac ttcttcgtag cgttttttca tcggcagttt gagattgaaa
    33781 atggtttcac ggcaccagcc attaaccagc cactgcgcca tcaatgccgc aactttagcc
    33841 ggtttttcaa ccatatcgca taccatccag gagatattgc tgcgtgtcgg acggaatttg
    33901 aaaccgtctt cccgcagcca cgtcacctgc ccggtatcca tcagactttg cgccatcggg
    33961 ccgttgtcga cggaataaac ccacatgttg cgcttcacca gttggtaggt ccagccgcca
    34021 gggcaagcgc ctaaatccac cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc
    34081 gcaggaataa acacatgaaa tgcctcttcc agtttgagcg tggaacgact cggcgcctct
    34141 gccggaaatt tcaggcgcgg aatgcccata tagaacggcg aattattgtt gctgtatgag
    34201 taaccggtat agcagcagcc tggtgcaata aagaatacat gcacaaccgg acgcttcggc
    34261 gtttcatagt tcgccagcac cccggcatcg cgcaaggcag cgcgtagcgg cacggtgaat
    34321 ttacggcaga atttcagcaa ctctttgctt tcgttggtat cggcaacttc aacacgcagt
    34381 tcaccgccct tctctactac gccctgcaac atgtcgacaa tgggggtaat acgatcttct
    34441 ggcggcaaat gctgcaagag ttcccccacc acaaaccact ggcgggcaaa aattaatgaa
    34501 ctgaacggca gctcacggat taacttatcg ccatcatcag gttgataaca ttcataaatg
    34561 acataacccg cattctcttt cacgcgggca aaaccgaaaa tttcccgctg gccggcttta
    34621 tcggtaattt ctgcggcgca ctctttttca aagcccggac ggcacagcaa tacaacctta
    34681 ttcatgactt acgcccttgc gctttaaacg gatagcacca actaacatta acgcccagcc
    34741 cgcgaggaag ctcacgccgc caaccggtgt gacaaatgcc cacaaacgca gatgggacag
    34801 cgccaggcaa taaaggctgc cgctgaacaa caccgtgcct aacgcgagga aaacgctact
    34861 ccagtaaaac cagatactga tgcgacgctg cattgccacc gccagaccta agatcgccag
    34921 cgtatgaaac gcctggtatt cgaggccggt ctggatccag cccatctcaa cggcccccat
    34981 ggttttactt aacacatgcg cgccaaaagc gcccagagcc acaaaaatga agccgctaat
    35041 ggcggcgaaa atcagcataa aacggctggt catggtcgta ccctacgtaa attattgttc
    35101 ataacgaaag cggaattttt cttgttcagc agcggctttc gccaggatcc attggcgaaa
    35161 ggcggctatt ttacccagtt ctgcctggct gtcatgacaa accagataaa aagcgttttt
    35221 actgaccaga acatcattaa acgggcaaac aagacgtccg gcctcgattt cagattgcgc
    35281 catcacgtta tttgccagcg ccactccctg cccgtgaata gccgcttgca gcaccatggc
    35341 gctatgacta aaaattggcc cttgctgaac gttgatatga tttaacccca actgtcgggt
    35401 atatgtctgc cagtcacggc gtgacgcatc atgtaataac gtatgtttag ccagatcttc
    35461 cggggtcttc aagggttttt cgcctgtcag cagtagcggc gaacacaccg gcaataaata
    35521 ttcggcgtac agtttttcca cccgtagccc cggccagttg ccccgaccat aaaatatcgc
    35581 cacatcaaca tcatccgcca gcttatcttc ctgacgatca accgcctgga ttcgaacgtc
    35641 aattcccgga taagctgaat taaagctgga aagtcgcgga accaaccaat gaatggcgaa
    35701 actggggagt aaactgaccg tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt
    35761 cgcttcggtt aattgcgaaa atatctcttt gatatcgagg aaatagcttt gcccttcctc
    35821 ggtcagcagg agtgaacgat tacggcggcg gaacagtttt agccccaaaa aatcctcaag
    35881 agacttgatt tgatgactta ctgcggcctg ggtcacgaaa agctcttctg ctgcgcgagt
    35941 gaaacttaaa tggcgtgctg cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg
    36001 tttagacatg gcttttaaac tttgatgtta aattaattta acaattagat cacactgtgt
    36061 aacctattag tttttttaat ctgagccatt ataaattgtc cgttgagctt ctaccagcaa
    36121 atacctatag tggcggcact tcctgagccg gaacgaaaag ttttatcgga atgcgtgttc
    36181 tggtgaactt ttggcttacg gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga
    36241 ttcagaccat ggtagcaaag ctaccttttt tcacttcctg tacatttacc ctgtctgtcc
    36301 atagtgatta atgtagcacc gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat
    36361 tactgatcca gttcgaccat ctctttcacg tcagtacggt tgatctgctg tttgttgcca
    36421 ttagcgtctt tatacgaaat cataccggta tcgttatcag tctgtggttt gccgtcagag
    36481 acgatggtac gtccgtcatt ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg
    36541 gcaaaagtca gcatacaggc agaaataatt gcggcagtct ttttcatcat cttctcctta
    36601 aagcgattaa taaatcttaa tagtattgat taacaggcta aaattaacgc ctaacactat
    36661 tcagcatatg ttacttacgc ggcttcgcca ggatatccag ataattctga tggttagcac
    36721 tctccttgta tcaaagtgaa ttttgcgtca cgatcggtgc atcaagccga ggagtaccat
    36781 gaacgttttt aatcccgcgc agtttcgcgc ccagtttccc gcactacagg atgcgggcgt
    36841 ctatctcgat agcgccgcga ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca
    36901 gttttacagc ctgagcgccg gaaacgtcca tcgcagccag tttgccgaag cccaacgcct
    36961 gaccgcgcgt tatgaagctg cacgagagaa agtggcgcaa ttactgaatg caccggatga
    37021 taaaactatc gtctggacgc gcggcaccac tgaatccatc aacatggtgg cacaatgcta
    37081 tgcgcgtccg cgtctgcaac cgggcgatga gattattgtc agcgtggcag aacaccacgc
    37141 taacctcgtc ccctggctga tggtcgccca acaaactgga gccaaagtgg tgaaattgcc
    37201 gcttaatgcg cagcgactgc cggatgtcga tttgttgcca gaactgatta ctccccgtag
    37261 tcggattctg gcgttgggtc agatgtcgaa cgttactggc ggttgcccgg atctggcgcg
    37321 agcgattacc tttgctcatt cagccgggat ggtggtgatg gttgatggtg ctcagggggc
    37381 ggtgcatttc cccgcagatg ttcagcaact agatatcgat ttctatgctt tctctggtca
    37441 caaactgtat ggcccgacgg gtatcggcgt gctgtatggc aaatcagaac tgctggaagc
    37501 gatgtcgccc tggctgggtg gcggcaaaat gattcacgaa gtgagttttg acggcttcac
    37561 gactcaatct gcgccgtgga aactggaagc aggaacgcca aatgtggctg gtgtcatagg
    37621 attaagcgcg gcgctggaat ggctggcaga ttacgatatc aaccaggcag aaagttggag
    37681 tcgcagcctg gcgacgctgg cggaagatgc gctggcaaaa cgtcctggct tccgctcatt
    37741 ccgctgccag gattccagcc tgctggcctt tgattttgct ggcgttcatc acagcgatat
    37801 ggtgacgctg ctggcggagt acggtattgc cctgcgggcc gggcaacatt gcgctcagcc
    37861 gctactggca gaattaggcg taaccggcac actgcgcgcc tcttttgcgc catataatac
    37921 aaagagtgat gtggatgcgc tggtgaatgc cgttgaccgc gcgctggaat tattggtgga
    37981 ttaatgacga acccgcaatt cgccggacat ccgttcggca caaccgtaac cgcagaaacg
    38041 ttacgcaata ccttcgcacc gttgtcgcaa tgggaagata aatatcgcca gttgatcatg
    38101 ctggggaaac agcttccggc tttgccagac gagttaaaag cgcaggctaa agagattgcc
    38161 ggatgcgaaa accgcgtctg gctgggatat accgtgactg aaaacggtaa aatgcacttc
    38221 tttggcgata gcgaagggcg cattgtgcgc ggcctgctgg cggtgctgtt gactgccgtt
    38281 gaggggaaaa ccgccgccga attgcaggca cagtcgccgc tggcattgtt tgatgagctg
    38341 ggattgcgtg cgcaacttag cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg
    38401 attatcgctg cgacgaagca ggtttaagca tagaaccggg tcggataaga cgttcgcgtc
    38461 tcatccgacc tgattgttta accctgacgc gccgctttcg ccatcatctt cttcagcgca
    38521 tgagaaaccg caacaaaccc aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat
    38581 gcacaatcca tccgcttcgg cccttctgcc gtggctttca tcgcacacac agtaccgtct
    38641 gactgcgggt acaccagcgc ttcagtagaa aacacgcaat cgacgccgag cttaccttta
    38701 ctgtttttca ctacgccaaa atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga
    38761 tcctgaatcg ttttcgccag atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc
    38821 gcgccaccgg tcgtgaccag cgggatttta ttgcgccgac aataagcaat cagcgccgct
    38881 ttgggccgta cactatcaat ggcatcaatc acgtacgaat aacccacgct catatactgc
    38941 gctacgttat ccggcgtcac gaaatcatcc accaccgtta cacggcactc cgggttaatc
    39001 tggcgaatac gctccgccat gacttccgct tttgccagcc cgacgttatc gcgcagagcg
    39061 tgaatttgcc gattggtatt ggtgacgcac acatcatcca tatcgataag cgtgattgcg
    39121 ccaatccccg tgcgcgccag cgcttccgcc gcccaggaac cgacaccacc gataccaacc
    39181 acacaaatat gcgcgtcagc aaacagttgc aacgcttttt caccatacag acgcgctgtg
    39241 ccaccaaaac gctgacgcca tgcatcacta attaccacag acatagcacc tcagaattaa
    39301 aaagggtgag gttatccctc accctgtcgt atccgtaaaa acggtataca gaataacaca
    39361 tcagccgcta aagacgttac ctgcgcccgg ggcggttttc agcacccaga cacgtccata
    39421 gtggttgtac caacctgcgc ggtgtccggc ttccggcccg atcccttgat agatatcgaa
    39481 gtgttggcct ttgattgcac caccgacatc cagcgccacc atcagacgca gttcgtactg
    39541 accattaaat ttgccgttat tatccagcaa cggcacttct gccagcaagg tagtacctgg
    39601 cggaataatg gaacgatcag aggcaactga cgcgcgacca accagcggca ccgcacttgc
    39661 ccctttcacc ggtgcaaaag attgcggttt aaagaagacg aaagacgggt tctgttccag
    39721 cagctcgcga acctcggctt cactgtgtgt ttcgccccag tgacgaatcg cctgcatcga
    39781 catatcttct tttttcactt cgccacggtc gatcagcacc ttaccaatgc tgcgataggc
    39841 atgaccgttt ttccctgcat agctgaaaaa gttaagcgga ctgccatcac caaagtcgat
    39901 atacccacta ccctgcacat ccataatgaa gttatccatc agggagttac tgtaagcgag
    39961 aatatattta tcactcaatg cccccgcgta gatctccgca cgagacggca gacgaccacg
    40021 ttttggcggc atacggtaaa taggatactg gaactcgccc tggcgggtat ggcgcgcctg
    40081 aattaccggc gtgtaatagc ctgtaaactg cacgttacca tagttgtcgg caccttccat
    40141 ctgccaggca tcaatgccga actggcgcat attgcgggta tcaccgcctg cgcgcagcca
    40201 ctcttgcacc gcgttataaa cattactctg gttgccatac agacgcggtg acgaattacg
    40261 gatatggtta atttgctcgg caaaatcacc ggcgttaatc ggcgcgccaa cggcatctgg
    40321 ctggttcacc agagagaaag gctgggtaaa tttcccgtct ttatattgct gtccgcgatc
    40381 ggttggtttg gaagagcagg cggcaagcat tgccacaacc gtgcccataa gaaggtactt
    40441 tacccaacgt cctttcattg ttctcttctt caggttaaaa aataaggcgc aacgaagata
    40501 acaaaccgcc gcgtttaatg aaatgagcag cctctccctg acgcaaattt tgcacaaaaa
    40561 ataggcttta gtgatttgtt tttgttcaaa atcatgccag atccgtgatc ggggtaaaaa
    40621 aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc cacggaggcg gggtggagca
    40681 gcctggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa
    40741 ccaattattg aacaccctaa cgggtgtttt tttgtttcta ctctcccata aaaaaagcgc
    40801 cattcagcgc ctttttatca tccccttctc gccagcgttg ccccatcggc aaaatacgct
    40861 ttaatccccg caagaataga ctccgcaact tcctgctgga aagtcgccgt tttcagttta
    40921 cgctcttcct caacgttact gataaacgcc gtttcgacca gaatggaggg aatatctggt
    40981 gcctttagta cggcaaaccc ggcctgttca acttgatttt tatgcagctt gttgatttta
    41041 ccgagcttat tcagcactgc tttaccaaac ttaaggctgt cagcaatggt cagcgactgc
    41101 accatatcga acatggtgtg gtcgacatag cggtcaccgc ttttgctcac gccaccaatc
    41161 aagtccgagg cgttctgggt ttgtgccaga tattttgccg cagtactggt tgcacctttg
    41221 gttgagagcg caaacaccga ggaaccgctc ggctggcgac tggtaaaggc gtcggcatgg
    41281 atagagacaa acaggtcagc acgctgtttc tgggcttttg ctacgcgcac ttgcaacgga
    41341 atgaagatgt cttcattgcg cgtcatgtac accttcatat tgccctcttt ctcgatcaga
    41401 gagcgcaaac ggcgagctat ttgcaatacc acatcttttt cgcgcgtttt gtatttcccc
    41461 accgcaccgg agtcttcgcc accgtggcca gggtcaagca taatgacaat cggacgatcg
    41521 cgccctgctt tacctggttg tggaccactt tgcgctggcg gcacctgctt ttcgaggtcg
    41581 cctttgttgt aatcctccag cagcgccagc agcgggtcct gcatatcctg tgcattggcc
    41641 ggatagaggt ccatcaccag acgctcttta aacccggcga ccggcgcaag ggcaaacagc
    41701 tgcggtttta cgttttgctt taattcaaaa accatccgta cggtttgcgg gtcaaattgc
    41761 ccgacgcgcg ccgacttgat gaacgggtcg tctgcacgaa tttgtgccgc catccccttg
    41821 agtaccgagt tcaggtttac atcttcgata tccaccacca cgcgttcagg gttactcaac
    41881 gcgaactgct tatatttcag ctgacgattt gattctaccg tcacgcgggt gtagctggac
    41941 gcaggccaga cgcgcaccgc cacgacctgg ctaaccgcag ccaggctgac ctgacttacg
    42001 ctcaatagcc acatagcgcc cgcgccttgc agtaaacgac gacggctgat tgcagtgttg
    42061 gatcctgaca tgcctctccc gagcaaaaga aatctaagct gtgtaacaag taaacgacta
    42121 atttgaccgg aaactttagc gaaagacgca taatctgtca tctaataaac ggtaaacatt
    42181 ctttttatat tcacggcatt actgataaaa aagtcgctct cgcataaaat ttacacttgc
    42241 accctgcgaa aaaacagaat aaaaatacaa taatttcgaa taatcatgca aaggggtgtg
    42301 ccgtggtaaa ggaacgtaaa accgagctgg tcgagggatt ccgccattcg gttccctata
    42361 tcaataccca ccggggaaaa acgtttgtca tcatgctcgg cggtgaagcc attgagcatg
    42421 agaatttctc cagtatcgtt aatgatatcg ggttgttgca cagcctcggc atccgtctgg
    42481 tggtggtcta tggcgcacgt ccgcagatcg acgcaaatct ggctgcgcat caccacgaac
    42541 cgctgtatca caagaatata cgtgtgaccg acgccaaaac actggaactg gtgaagcagg
    42601 ctgcgggaac attgcaactg gatattactg ctcgcctgtc gatgagtctc aataacacgc
    42661 cgctgcaggg cgcgcatatc aacgtcgtca gtggcaattt tattattgcc cagccgctgg
    42721 gcgtcgatga cggcgtggat tactgccata gcgggcgtat ccggcggatt gatgaagacg
    42781 cgatccgtcg tcaactggac agcggtgcaa tagtgctaat ggggccggtc gctgtttcag
    42841 tcactggcga gagctttaat ctgacctcgg aagagattgc cactcaactg gccatcaaac
    42901 tgaaagctga gaagatgatt ggtttttgct cttcacaggg cgtcactaat gacgacggtg
    42961 atattgtctc agaacttttc cctaacgaag cgcaagcgcg ggtagaagcc caggaagaga
    43021 aaggcgatta caactccggt acggtgcgct ttttgcgtgg cgcagtgaaa gcctgccgca
    43081 gcggcgtgcg tcgctgtcat ttaatcagtt atcaggaaga tggcgcgctg ttgcaagagt
    43141 tgttctcacg cgacggtatc ggtacgcaga ttgtgatgga aagcgccgag cagattcgtc
    43201 gcgcaacaat caacgatatt ggcggtattc tggagttgat tcgcccgctg gagcagcaag
    43261 gtattctggt gcgccgttct cgcgagcagc tggagatgga aatcgacaaa ttcaccatta
    43321 ttcagctcga taacacgact attgcctgcg ccgcgctcta tccgttcccg gaagagaaga
    43381 ttggggaaat ggcctgtgtg gcagttcacc cggattaccg cagttcatca cggggcgagg
    43441 ttctgctgga acgcattgcc gctcaggcga agcagagcgg cttaagcaaa ttgtttgtgc
    43501 tgaccacgcg cagtattcac tggttccagg aacgtggatt taccccggtg gatattgatt
    43561 tactgcccga gagcaaaaag cagttgtaca actaccagcg taaatccaaa gtgttgatgg
    43621 cggatttagg gtaagcgatg aaaatcgccg gatgcggcag gcgtaacact cgtgcgtcgc
    43681 atccggcaat tacgtttatc cccgcgaact aaacaacgcc gccagaccac tgcgccgctc
    43741 agtacgagtg gcgattgccg cacttaatat acgctcatcg gcatacagcg acagacggcg
    43801 acgcgcgcgg gtcaccgcgg tgtataccag ctctcgcgtt actatcggcg tgcgttggct
    43861 cggtaaaatc aacgccgcat ggtcgaactc cgatccctgc gatttatgta ccgtcatcgc
    43921 ccacgtagtt tcgtgctctg gcaagcgact cggttgcaca gacttaatat tgccgtccgg
    43981 catcgcaaac cagacgcgcg tcccctgccc gcgatccagc gcaataccga tatcgccatt
    44041 aaacaaccca agcgcgctgt cattacgggc aatcatcacc ggtcgacctt cgtaccaacg
    44101 agagtgcgaa tgacgatgaa ttttgcgctt ctgttgcata aactgctcaa ttcgctcatt
    44161 cagtccagcc acgccaaatg gcccttcccg cagggcgcac aaaagttggt actcattgaa
    44221 cgcctgaatg attaaatccg gctcggcacg cgcttgcagc agatccagat aacgtccata
    44281 acccgcaaga gcttcctcaa gcatcgcaat ataatcttcg ccgctctgta aaagccgttt
    44341 ttcaatatca gtaaaatcct gctgaaaaac ggttttcact gccgttttat caccacggtt
    44401 gatcgccgca gctaactgac caatgccaga atcgctgccg aaacgatagc ttttttgcag
    44461 caggcagaga ctgtcgcgca aagatgccgc ttctgtgccg gttcctgccg gaacgtgagt
    44521 tcccgtcagg cggcttagct gcccggcacg ctcggcggta aagccggcat tggcataagc
    44581 gcagatatcg cccagcacag ccccagcctc aaccgaggcc agttgatcac gatcgccgag
    44641 aaagatcact cgcgcatgat cgggcaaggc gtcgatcagt ctcgacatca taggcagatc
    44701 gatcattgac gcttcatcta ccaccagcac atcaagatgc agcgggttac cggcatgatg
    44761 acgtaaacgc tggctacccg gctgcgcgcc cagcaatcgg tgcaaagtgc tggcatcttc
    44821 cggaatgcgt ctcttttgtt catcggtcag cggtaactgt cgcaaagcct tgccgagaga
    44881 ttcggttaag cgcgcggcag ctttacccgt tggtgcagcc agacggatac ggcagcgttc
    44941 gccgtcggcc atttgaatta acgctgccag caacttcgcc acggtggtcg ttttaccggt
    45001 gcctgggccg ccggaaatca ccgagatccg ccgcgtcagc gccactgctg ccgccacttt
    45061 ttgccagtta atttcatcgc ttactggaaa aagtttgtcc agggtttgcg ccagtagagc
    45121 ttcatcaacc tcaatggcat gattcacctc attgaagaag cgcgctaccg tccgctcgtt
    45181 acaccacatg cgattcaaat aaagacgatc gccacagagg atcatcggcg tgggttcatc
    45241 tcccctgctg accgcttgag aagccagcaa ggattcttcc caattttgta gctcaccgat
    45301 ttcactgaca caggtcgcca acagcggatg cgacgcctcg ttattttcca gtcgtgaaag
    45361 cggcaaacag acgtgtccct ctccggcatc atgacttaac agtgccgccg cgagggtgac
    45421 ggcaggatgt tcatctcccg ccacggtcag ggcaaattgc acatccagcg ggcgtagctg
    45481 tttgtgctcc acagcttcca gtaattgctt ttgcaatttc attacgcctc ctccagggtc
    45541 ataccggcaa acatctcatc catcagggca atcaacccgg cgttgggtcg ggttgtgtaa
    45601 atcccttgtt gcggatgttc tttatcaacg ccacgcagga acagatagat aacgccgcca
    45661 aagtgacgat catagtcgta atcagcaatg cgatggcgca gatagcgatg cagcgccagg
    45721 gtataaagct gatattgcag atcatagcgg tgtgcctgca tcgctgctgc catagcctgt
    45781 tgggtgtaag ccgaactgtc ttcacccagc cagttggatt tatagtcgag caggtaataa
    45841 cgcccttcgt ggcggaatac caggtcgata aagcctttta acatgccgcg tacctgcatg
    45901 aactccagcg gcgggcagcc agcggatagc gggtcaaact ggcgaattag tgcatcaagc
    45961 tggctggcga tgagcggttc gctaatcggc aggtaaaact ccatctccac ctgtttatcg
    46021 cggtcggaaa gctgactcag gctaacaccc gtttcattga gaggtgcctg gaggacagcc
    46081 gtgatccact cggtcaacac cggttcccac tgcgattcaa agccgccgag ttccagtttt
    46141 tcctgtaccc agttcgggtc aaccggctgg gtaaaatcga ggtcttcaaa caaactgtgc
    46201 aagaatgtcc ccggtgacgc accgcgcggg aactgatgcg gtgttaacgt cggttcttca
    46261 acgacgctga ccacgcctgc ggcatcaaca tccagccgtg gcatcagatc ctgagcgata
    46321 ccgtgaccac gctgctgcaa gccggagtag ctggtgacac gccagttatc gccgggcaat
    46381 cgttgtagtg tcctcgcatt cagttctgca gttaaagcat cattaacctg ccagggctgg
    46441 ttatcaccag tttgtgccgt ttgccaggca atatcatcat cgcataacgc ttcaatacag
    46501 gtgcgaagcc ctgccgcatc ttgcggttcc cctttttgca gcaaacgccc gagcgcactt
    46561 tggtggacgt cggtgtcacc ttttttatcg ccacgacggc gcaccagcgg tgcaacgccg
    46621 agactgcaat gccaaaccga acgcgtcagc gccacgtaaa gcaaacgcag atcttccgcc
    46681 agacgttcga cctccgcgag gtcgacgctt tctggcgcag cattaagatc caaaactgcc
    46741 tcaaacgagt ggcgatcgtg ataaaacgcc tgatcctgga cgcggaaatt ggtaataaat
    46801 ggcaaccaga ccagcggata ttccagccct ttcgatttat ggatcgtgac aatctgcacc
    46861 agatgtttat cactttcgag gcgcatttgt tggctggagg cattactgtc tggctcgagg
    46921 atatgttgcg ataaccagcg taccagcgca tgttcacttt ccagctgcgt tccggcttct
    46981 tgaagcagtt cgctgatatg caagatatcg gtaagacgcc gctcaccgcc tgccgttgcc
    47041 agcaagtttt ctgcaatgtt acgcgccgac atcagcgccc gcagcatcgg cataacgcca
    47101 cgtttgcgcc agatttgccg ataaccatcg aactcttcga ctaccgcatc ccacgcatgt
    47161 tcgtcattat tcagcgtttc aatatccagc gcattcagcc ccatcattga cgttgccagc
    47221 gcactgcgca gggtgttctc acgttcgggc gtcatcaccg cctgcaacag ccaaagcatt
    47281 tcctgcgctt ccagagtttc aaaaacacta tcgcggttcg aaaggtaaac ggaagggatt
    47341 tccagcaacg ttaaggcatc gcgcacctgg gcggcctcct ggcggctgcg caccagcacg
    47401 ctgatgtccg aagcacgcac cggacgcgcg tcgtcgccgt tcatcagcaa cgcttcgccc
    47461 cgctgtccgg cttgtagcca gtcgcggatt tgcgcagcac atacctgcgc catggtactt
    47521 tgatagtcgc caacgccgca gctttcgcct tccatcagcc acattttcat cgcaggctgc
    47581 gtttcacctt taaagacaaa acgtaacgcc tgatttttcc cggctgattt cactggaata
    47641 aacggtattt cgcgaaacat gaacgcgtca tcagtctggc tgaaaagctt gttcacactg
    47701 ttcaccattc caggtgcgga gcgccagttg gtgtctaaag tgtagtgggc gtgaacttca
    47761 ctacgcgcct tcatataagt gaagatatcc gcaccccgga atgcatatat ggcctgcttc
    47821 gggtcgccaa tcagcaacaa tgcggtttct ggctgatggt gccagatacg gcgaaaaatt
    47881 cggtactgct gggggtcggt atcctgaaat tcatcgatca ttgccaccgg gaatcgcgta
    47941 cggatcgccg ctgccaacac ctcgccgctt tcgctacgca gcgcggaatc gagccgactt
    48001 aacatgtcat caaaacccaa ttcgccacgg cggcgttttt cacgcgctac tgtttcgcgg
    48061 atctcagcca atgcgcgggt gatcaccaga tcacggatcg acaatggttc tgcaagcagt
    48121 tgatcgatcg cctcaaacag tggatgtcgc ggggtttccc ccccggcttt cgtgcgatct
    48181 tctaagaaac gctgggagaa tttttccagc gactccggca actgataact gtttgtctct
    48241 tcttctgccc aggcgctgat cttgtcgatc catttagcct gattgctacg gttaaacttg
    48301 cgtcgatcaa taccagaaga ttcgatcagc gcatccagtt cacccactgc gtcgcgccac
    48361 tgctgtttta ccgtatcaat acgcgccaca atttgcgcgt gacgggaagc tagcgtttca
    48421 tcatcgggcg gcggtgcttt gataaccggc gcttcgcctt gcagataacg attaatatcg
    48481 cgcagcaacg cctgcggccc tttccaggtt tcaaagacga cctgggctat ttcacgcggc
    48541 agcgggtagc agtggcgacg ccagaaatcg gcgcaggcct ggtagcgtag cagagactca
    48601 tcttcaatca gctgctgctc aaacagcatg ccggattcaa aggcattcag gttgagcatg
    48661 cgctggcaaa agccgtgaat agtaaagact gccgcttcat ccatctgccg ttcggctaac
    48721 aacaaccact gcgcggcttg cgctttatcg tcgacctctt ccagcaggtg ttcgtacaat
    48781 ggattgtcgg tggtttcacg cagacaggcg atgcgcaact cgtggatatt gctacggata
    48841 cgaccgcgca attctgccgt ggcagcctcg gtaaaggtga ccaccagcag ttcttcaacg
    48901 gtcagcgggc ggggaaaggc ggcggaaccg cctagtccaa gtaacaggcg caaataaagc
    48961 gccgcaatcg taaaggtttt gcctgtgccg gcagaggctt caatcaggcg ctcgccctgt
    49021 aagggcaagc gcaaaggatc tagtgtctcg gcgacatcac tcattctttt cactcatcag
    49081 gggcattgtt tgctgcaacg cgctgacgtt ctcccacact ttccagcctt caggatgcac
    49141 atattcggct ttcccgtttt ggctgccgga aatctgcgac aggatagcca tgccttgcgg
    49201 ctcgactacc gcctgatgga agaaatcggc cagtttttgc ggcgtcagca gtttaatctg
    49261 ggccacgatt ttatcacgcg aatcgaagcg catattgccg cgatcgaaat ctttacttaa
    49321 cttcgatgct tcttcgccga gcgtttgcgg tgcctgcagc atctgggtaa ttaccgcctg
    49381 ctggatttgc gcaaactcat ctggcttcat cgctcgcaat tttgcctctg cggttgggaa
    49441 aaacgccttg taacgctccc acaagaatga aggctgttta tcattgcttt gcaaaaggaa
    49501 gcccatgccc cactgacgcc ccacgctcat tggaaacgca aacacggcat agcccaattg
    49561 ttcttcggta cgcaactgat tgtagaacca cggctgtacg atctgcccca acagagagct
    49621 ataggctgag ctggtgtatt catcgtagcc agtcggtaca aataccgctg ccagtgcgga
    49681 gtcggtgctg ttaccggctt tttcaaagat gacggattgt tttttatcga ccactacatc
    49741 tttgtttcga caccactctg aaccatcagc gcccaactgt ttttgcacat cgcgtgccag
    49801 cgttgttgcc tgggcctcgg tcatgttgcc gataaccata aactctggtc gagcccctga
    49861 ttttaaggcg tcgcgataag ccagcacctc tttcaacgta atggagggca aaattttacg
    49921 ccgttcatct cgcgagaagt acggcacttg cgagagcatc tgcgcgggca taatcgcctg
    49981 ctcaaacgct ttaccctttt ctgcggaatc catcatctgg ttataccagg acttcgcctg
    50041 ctcaagctga tcttccgtag cggtatagct aaagtacccc tcgagcaatg cctggaacag
    50101 ctgcggcaga cgctgggtgt aaccattagc attaaccata aggccgttgt tagcgttggt
    50161 ggaaaaactt atgccaccaa ccgacgcctg gttgcttaac tgatcaagcg ccagccctgc
    50221 gagataatca ttgagcgcaa acatcacctg attgcgggcg ctgtccatgg ctttcggatt
    50281 acgcaaaatc aggctgacat cagctttggg ttcgctggaa aaataacggc ttggcgcata
    50341 caccacgcgc agattcgact catcaacaat cagctctgga tggtcatatt tcttctctga
    50401 cttaatcagc gagaaatcgt caggaatata ggggttaagc tccggtaagg agagcgcaat
    50461 attggcagct ttttgctgcc agtcagcgaa agtttgttcg ctgattttat cgacctgata
    50521 cggcgcatcg acaaaataag ccgttttatt gtgcggctct ttcgggctga tataccagat
    50581 acgcgcattc tgcggcgtca tcatcgccag acgttctttt actgctttag catcgtaccg
    50641 atcggcaata ttgactgcat ccagcgtatg ctcaaccgga acgcgaatca tggtatctgc
    50701 cagccattcg acgtaatcca tatcacgggt gatcgacgga taacggaagt cgatatccag
    50761 cacattcgcc agttcatcga agtattgttt atcaatgcct ttttcacgta acagattgag
    50821 atagctaaaa attgccgcca caacctgatc gcgattagcc aggcctttat cggttaaaga
    50881 cgcagagatc gctaatacgc cgctgttgcc gttgacgata ggatcggagt tggcgctaat
    50941 gccctcaact aatccctgct tttgcagcca gtcggaaagt gtacctgggc tgcgattacc
    51001 aatcagatag gtaatcaatt catccgtttt actacggaac ttcgctgagt tgttatcgat
    51061 gcgaaactca acgcgcagaa ctttacgcgg cattgccggg acgtaatgaa tgataatgcc
    51121 cttttgcgcg tcggtgacta ccggcacggt gatttccggt tttttgctct ctttgttcgg
    51181 cacgcgacca aaggtgtccg ccgccatttt tgccaactcc ggcagcggtt tattgctgta
    51241 aataaccgcc ttcatcagat tggcggaata gtacttctcg tggaaatctt tcagcgcctg
    51301 ctgcaccgga ttaccaggtt tatcgcttaa agtttcgagg ttaccaccag aaaactttga
    51361 accggggtgt gccgggttaa tggtttctgc gctgacctgt gccatgcgca tcccgtcacg
    51421 cgtacgcgcc atggttaatt cagcgttcac cgcattacgc tcacgttcgg catatttctt
    51481 gtcgagcaaa ggttcagcaa tagcatcggc caggcggtct accgcaccag gcaaggcgtc
    51541 gttctcaact tccagataga aagccgtgcg atacggcgca gtgctggcat tgtgactacc
    51601 gccgtgcatt ttgagatatt cggccagact gtcagcctgc gggtactttt tcgaccccat
    51661 cagactcata tgttcaaggt aatgtgccag cccctggtac gcctcgggat cttccagcga
    51721 cccaacgggc accaccagcg ccgagagcga tttaactgcc tgcggatcag aaaccagcaa
    51781 gaccaccata ccgttatcca gacgtatagc ctgatactgg cggttatctt tatcactttt
    51841 acggatggtt tcctgaatcg gctgccatcc cgtttctgcc tgacttaagg gtgcccaaag
    51901 ggcaactaac aacaataatg ctttgaacca ggtgctgcgg ggcattctcg gacctcataa
    51961 tcaacttaat tttctgtcca gattcaacac gttaacgcaa atcatctgcc agaatttaat
    52021 cttgtgctgc acgagtcagc ctatgtttat ataaccatca gtccgtgact ggtgcgcatc
    52081 ataaagtaag cggatagatt gcgcaatttt tatacagcac tcatgactga ttaaagcgaa
    52141 acagcggtaa caggaaacgt tgcgactgtt caacgatggc ctccattgtc tctggtgtta
    52201 attgccgcca gagcctttga taccagatat catcaccttc gccacgcacc atcatgttac
    52261 cttcgtaagc ctgaaggaat ttcgtacggg ctttttgcaa cgtggaatcg tcatccagca
    52321 tggcatcgtt ttgcgcgtca taacaggttt ttagccacgc gccgccactt tcaggtaaca
    52381 ccagcaatgg cgcggacatc ccttcacgat acccctcaat cagttgtgag aggtaatgca
    52441 aagcctgttc ggctgcaagc ggcggaaaac gccactcgcc gtctttgcgt agaaaaaggc
    52501 gactttcacc attaccaccg ctggcacagt agacaaggtg ttccagccaa agttgcattc
    52561 cctgcgccac acttaataaa gagggacgcc agcgcaacag gccatccggc tgcacctgcg
    52621 gcaaccagcc agttatctgc acaccgttgc aggcgagatc aatttccata ctctgccccg
    52681 gctggcgaca ggcaatgact ctgtcggcaa gctgctgcat ctcctggcac tgagtttccc
    52741 agaaaatttc accaaaagcg ccatacggta aatcccctgc cgcccggaag cgacggaaca
    52801 agcgttcggc atcatcctgc tcaaccagtg cattcaataa ctgctgattg atttgataac
    52861 ggctaagccc ttccagaata aatggctcgg tgtcggggat ttcgctgtct tcagtacgga
    52921 agttcacctg caaacgcatc tggaagaacg cccgcaccgg atgtgcccag aatcgttgta
    52981 gcgtttccag cggcacggtt tccggtaagg taaacggcag cggctgaaca aattcagaat
    53041 gtgctttacc agcctggctg gccgcaggta gccattcacg agcatagctt tgtcgttcgc
    53101 ctggctgata gttttgtgga tcaaacggca ttcgggtatg gaggcaagta agatgcgctt
    53161 ttacccttgc ctcgctttca tcacagttga gcgcttcatc gcccggtaga taatgacttt
    53221 gcccgatgta gtcgatcagt tcctgcacca gtaccgacgg gaaacgttca ctgttatcct
    53281 gaatggaacg cccgatatag ctgatataga gtttttgctg cgcggaaatt aacgcttcca
    53341 ggaacagata gcggtcgtca tcgcgacggc tacggtcgcc acgcttcggt ttctggctca
    53401 tcaggtcaaa gcccaatggc gcaagctgac gtggataaac gccgtcgttc attcccagca
    53461 ggcaaaccac tttgaacgga attgaacgca ttggcatcag agtacaaatg ttaaccggtc
    53521 cggcgagaaa acgctggctg atacgttctt gatccagacg ctgtgccagt tcatcacgca
    53581 atagtgacag cggcaccgcg tcgccatact gcgcacctaa accttcggcg ataatcgcct
    53641 gccattgttg ttcgatcagc gtcatcgccg cttcggtttc cgcatccggc aggaagaagg
    53701 cgctgagcat atcgcgacaa accggcaacc actcttctag cggacgctcc tgtgccagcc
    53761 cgcgacgcca gatgtttagc tgcattagca gtgaagccag atgccccacc agttctgcaa
    53821 ttaagccgct cgattcatca taaggtagaa ccgattgcca ctcgccctgc gcgctctcca
    53881 tcgcgtagcc cagcaacata cgcgtcaggc caaatcgcca ggtgtgttgt ccggtggcgg
    53941 ggagttccag ctcgcgaacg ttgtcgtcat ctatccccca acgaatgccg gattcgttga
    54001 cccactggcg taaataacgc agcccttctt cggtgatgtc aaaccgcgcc gccagcaccg
    54061 gcacatccag taatgccagc acgtcttccg acacaaagcg gctgtctggc aacgataaca
    54121 ggctgataaa cgcttccagt accggatgcg actgccgcgc ccgacggtcg gaaatggcgt
    54181 aaggcaggta acgatccgca ggtgcgctac caaacacagc ccgaataaac ggactgtagc
    54241 tgtcgatatc agccaccatc acgatgatgt cgcgcggagt aagtgtcggg tcttcttcca
    54301 gcatcgccag caggcgatcg tgcaaaactt caacttcacg ctgcgggcta tggcaaacgt
    54361 ggaaggtgat actgctatcc agtggatcaa gcgggcgttt gttatcgcta cgggaaaact
    54421 cttcgatgtt cacaccagca acggcacggt tttccagttc cagaatgtca gactgaatgt
    54481 tatgcagcag gttatctggc gtgacatcga caaaggcgtc cagctcctgg ctgctctcca
    54541 ggtcagaaag gagataaatg tagtcgcgcc caagcttacc ccatgaagcc agcagcgggt
    54601 tgccgacatc ctgttcacca tcgctgttaa agagctgccc ggcattttcg ctatcacgaa
    54661 atagcggcaa ttcgcgatct tcaaaactgt gtcgacgctg acgggtcagc agtttcgcca
    54721 gataagcagg atctttaata tcgccccagt aataacggca ggggttggta aacaggagat
    54781 ggatttcaat atgtttaccc agcgcctgta gcgcctggag ataaacaggc ggtaacgcgg
    54841 aaataccgca tataaagacg cgcgaaggta accccggcgg gcaggtcgtc gcggactcca
    54901 gcgtttcgat aaagcgctga tagagattgg cgcggtgcca gcgcggttgc ccgagttgat
    54961 gggtatattc caccagcgcc ttccacaacg gggcttgcca ggcctgtgct tctcccagcc
    55021 cttcaaccaa atgtcctgtt tcccactgtg ccagccagtc cggacgatag accagatact
    55081 ggtcaaacag gtccgccgct tttgaggaaa gctggaacag ttttcgcttg tcgctatcgt
    55141 cagtcagata atgccgcaac agggtaaagt cttcgcgctc cagcaattgc ggcagcagag
    55201 tcatcagttt ccagctcatg ctctgtttgt taaaggcgct ctctttgggg atttccggta
    55261 acacccggac gaacatatcc cagataaagc tcgctggcag cggaaaatca atgtttgccg
    55321 caatactaaa cttttgcgac agggtcattt gcagccactg cgccataccg gtactttgca
    55381 ccagaatcat ctctggttcg aaaggatcgt ccagccgttc gcgttcgaca ataaactcca
    55441 tcaacgcttc cagcacgtcc agacgattgg aatggtagac ccttaacata gcggctcctg
    55501 actactgacg attcgggcaa tgcaggcggg tcatctcgcc ttctctgccc ccgggtgaaa
    55561 ctagcgtaac gctgatgctg acacatcccg cctgcgatgt ctgcattcgg ttgacctgcc
    55621 agttggcagg tggcgaaatc gcgcgcagtt gcgtttgctg ccaaccatgc cgccagagct
    55681 gttggtactg gtttctgctg gcgagactgt tcattaatgt tcgctggata cccgataacg
    55741 cagtgacaat catcaccatc agcaccatcg caaccattac ctccggcagg ctaaagcctt
    55801 gttgattctt cagggaagct gacataacgc cctctctttc agcggacaaa aatcgctcca
    55861 gccgcgtggc gaaaaaacaa tgtttccatc aatgacttcg cctgttcgcc acaacgaaac
    55921 gccttcataa ccggcaacca ataaggcttc attatctgcc agtaaacgca aacaaacccg
    55981 ggcatcggtt ccagcgtact gcgagcactg aactgcgggc tgcgtctgcc aggagtgcat
    56041 tttcccccac gccagcgccg actgaacgat ggcctggcgg cgcaatgact gactttctat
    56101 gctcacgcga gaggcaaaac tgcgatcctg ctgactcatt ccttgcaata gcaagctacc
    56161 caaaaccagc agcatcagga ccagagccag tgacgaaaca cctttttcgc ggttcacagg
    56221 ttgaatcctg tcacgctata gctggcatcc accacggttt gcggttcaga cttactggca
    56281 gcacgcatat taaccgtcaa caccggcgag aagccgctga catcctgacg tacgacctga
    56341 aaagtgtcga taatgatggc atccggatta gtgactttat cccagccctt accttcacag
    56401 gatgtcgcac cgcgtagcgt ttccagcaca tgctccttca gacgaaatcc aatctggtca
    56461 gactctttta ccggttcgcg atcccagata ccgttactgt tcgcatccca ctgcacaatg
    56521 atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagctgcc atgacaataa
    56581 cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagc
    56641 gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc
    56701 cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct
    56761 tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt cttccccacg
    56821 gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg
    56881 cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg
    56941 gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg
    57001 aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat
    57061 gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg gcggtttgcc
    57121 atagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga
    57181 ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc
    57241 cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc
    57301 ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggcg tctcgaagaa
    57361 tttaacggag ggtaaaaaaa accgacgcac actggcgtcg gctctggcag gatgtttcgt
    57421 aattagatag ccaccggcgc tttaatgccc ggatgtggat cgtagccttc aatctcaaag
    57481 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
    57541 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct gtacaggtgc
    57601 gtatcgccac cggtccagac aaaatcaccc acttccagat cgcactgctg cgccatcata
    57661 tgcaccagta acgcgtagct ggcaatgttg aacggcaggc cgaggaagac gtcacaggag
    57721 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaatgcatgg
    57781 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
    57841 cgggaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
    57901 ccatctggcg ttggccaggc gcgccactgt ttaccataca ctggtccgag gtcgccgttt
    57961 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
    58021 tcgccctgca gaaaccacag cagttcgtgg atgatggaac gcaggtggca acgtttagtt
    58081 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
    58141 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
    58201 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
    58261 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg ccccatgctg
    58321 atgtactgca cccaggcacc ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
    58381 atgcgaaacg cgccgtagcc aatcaggaac aagccagaga cagctcccat tgggcgcggt
    58441 ttacgaatat acaggttgag gataataaac agcaccacac cttccagcag cagctcgtaa
    58501 agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga aaatggattg ccactgcggg
    58561 ttggtttgca gcagcaaaat atcttctgta cgggagcctg ggaacagcat ggcaaacggg
    58621 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
    58681 ccaagaccaa acggaatgag tggtgcgata aaatcagaaa cctggaagaa ggaacgttta
    58741 gtacggcggg caaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaagac
    58801 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaaattg
    58861 tagaacagaa cataaccaat acgtcccccg aggaagacgc cgaggaagcc cgcatagagt
    58921 aagttttcaa cttcattttt ggtccagccg ctgcccggac gattcgcccg tcgtgttgcc
    58981 agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg aagcgccacg
    59041 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
    59101 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
    59161 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
    59221 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
    59281 ccgcgacgct ccataaaggc tgcaacctga tggcgaactt cggtcgccag ttgcgcttcc
    59341 agactacgct gcgcaagatt ttctgcttcg gcataatcaa tgcgccgcag caggtatttt
    59401 acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
    59461 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat tccatgtatt
    59521 tccgcttccc gggcgatcat cgccagagct cgtaacattg caggatgaag actgtcataa
    59581 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
    59641 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa taccattgac
    59701 ggcacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
    59761 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcat caacttcatc gaggcttgtg
    59821 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
    59881 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
    59941 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
    60001 gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc
    60061 tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga
    60121 ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accagcattg
    60181 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
    60241 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtacc
    60301 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
    60361 agcaccgaag gctgaatatc cgcgcccatc acggtaggga tccccagcgc acgtaccatg
    60421 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
    60481 ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
    60541 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
    60601 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
    60661 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
    60721 aacaattcgc gacgcagccg ggtgtccgaa agtaggtgcg agtaaagatc gaaaatagcc
    60781 gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
    60841 gcttcttcca gcgccccggt cagtcgttcg cgttccagag ccggatccag cgttgatgcc
    60901 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
    60961 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa caaggcagtc
    61021 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
    61081 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
    61141 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
    61201 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
    61261 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
    61321 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
    61381 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
    61441 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
    61501 ggcgtgactt aacctctttt acgtcgataa gcagatgtgt tttgtggttt tggcgtattt
    61561 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
    61621 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
    61681 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag ctgcaagaga
    61741 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
    61801 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
    61861 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
    61921 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
    61981 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
    62041 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca agaagacggt
    62101 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
    62161 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
    62221 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
    62281 tagacatttt aaaatattca ttattagtca atgcattaca tgtaatttga gtaatgaaaa
    62341 agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat
    62401 gccgtattta tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt
    62461 ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac agttatccca
    62521 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
    62581 cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa aaagcaatat aaatcagaga
    62641 attgaacaac acatgtggaa aaagttacac tgcgaatatt cggcacataa ttgctgtttg
    62701 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actcctctct cctcccgaaa
    62761 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
    62821 cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg
    62881 tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg
    62941 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
    63001 cattcgtttg tgttgccccg ttaacgggta atagcggggt gacctgggaa accagccatg
    63061 tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggtgagcgc agcatcccgc
    63121 tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc gaatgaagag gaagaccgac
    63181 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
    63241 tcaccgctcg tcacggggag tatttacaga tacgaccgaa agcagcgaat gcgaaagcgc
    63301 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
    63361 tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc
    63421 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
    63481 gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta cgctgacgct
    63541 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
    63601 ccccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
    63661 tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc tttttacaat
    63721 attcagtcag gaaatttccg cccgtgattt gattctgctt ctgggtggct tgttccttat
    63781 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
    63841 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatcttcag
    63901 cctcgactcg gtgattaccg ctgtgggtct gtcagatcac ctgtttatta tgatggccgc
    63961 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
    64021 acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtcg gctttaccct
    64081 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
    64141 ctctattgcg gttgaaagcc tcaacctgat tcgcaacaaa aagaacccgc tctgatactc
    64201 cgtacgctct cctgcgggag ggcgtactca ctcctgttac cctcacagtt taagattttc
    64261 ctgctttcaa gatatataac gtcggtttat aaacagacta ttatcatagg tgggccaaat
    64321 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
    64381 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
    64441 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
    64501 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
    64561 tcagcatccg gctgtaatgc cgatcagtta ag
//
LOCUS       DAZPJR010000004        63003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPJR010000004 DAZPJR010000000
VERSION     DAZPJR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.005"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(1..413)
                     /locus_tag="ACHVB7_000260"
                     /pseudo
     CDS             complement(1..413)
                     /locus_tag="ACHVB7_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(519..3080)
                     /gene="mutS"
                     /locus_tag="ACHVB7_000261"
     CDS             complement(519..3080)
                     /gene="mutS"
                     /locus_tag="ACHVB7_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR1912369.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3156..3290
                     /locus_tag="ACHVB7_000262"
     CDS             3156..3290
                     /locus_tag="ACHVB7_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912370.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3367..3720
                     /locus_tag="ACHVB7_000263"
     CDS             3367..3720
                     /locus_tag="ACHVB7_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR1912371.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="ACHVB7_000264"
                     /pseudo
     CDS             complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="ACHVB7_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="ACHVB7_000265"
     CDS             complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="ACHVB7_000265"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR1912372.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="ACHVB7_000266"
     CDS             complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="ACHVB7_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR1912373.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="ACHVB7_000267"
     CDS             complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="ACHVB7_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR1912374.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="ACHVB7_000268"
     CDS             complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="ACHVB7_000268"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR1912375.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="ACHVB7_000269"
     CDS             complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="ACHVB7_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1912376.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9737..10198
                     /gene="hycA"
                     /locus_tag="ACHVB7_000270"
     CDS             9737..10198
                     /gene="hycA"
                     /locus_tag="ACHVB7_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR1912377.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10323..10934
                     /gene="hycB"
                     /locus_tag="ACHVB7_000271"
     CDS             10323..10934
                     /gene="hycB"
                     /locus_tag="ACHVB7_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR1912378.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10931..12757
                     /gene="hycC"
                     /locus_tag="ACHVB7_000272"
     CDS             10931..12757
                     /gene="hycC"
                     /locus_tag="ACHVB7_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR1912379.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCDYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12760..13683
                     /gene="hycD"
                     /locus_tag="ACHVB7_000273"
     CDS             12760..13683
                     /gene="hycD"
                     /locus_tag="ACHVB7_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR1912380.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13701..15398
                     /gene="hycE"
                     /locus_tag="ACHVB7_000274"
     CDS             13701..15398
                     /gene="hycE"
                     /locus_tag="ACHVB7_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR1912381.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15408..15950
                     /gene="hycF"
                     /locus_tag="ACHVB7_000275"
     CDS             15408..15950
                     /gene="hycF"
                     /locus_tag="ACHVB7_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR1912382.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15950..16717
                     /gene="hycG"
                     /locus_tag="ACHVB7_000276"
     CDS             15950..16717
                     /gene="hycG"
                     /locus_tag="ACHVB7_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR1912383.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16714..17124
                     /gene="hycH"
                     /locus_tag="ACHVB7_000277"
     CDS             16714..17124
                     /gene="hycH"
                     /locus_tag="ACHVB7_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR1912384.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17117..17587
                     /gene="hycI"
                     /locus_tag="ACHVB7_000278"
     CDS             17117..17587
                     /gene="hycI"
                     /locus_tag="ACHVB7_000278"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR1912385.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17612..18385
                     /locus_tag="ACHVB7_000279"
     CDS             17612..18385
                     /locus_tag="ACHVB7_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912386.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18547..18858
                     /locus_tag="ACHVB7_000280"
     CDS             18547..18858
                     /locus_tag="ACHVB7_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR1912387.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18855..19274
                     /locus_tag="ACHVB7_000281"
     CDS             18855..19274
                     /locus_tag="ACHVB7_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1912388.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="ACHVB7_000282"
     CDS             complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="ACHVB7_000282"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR1912389.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="ACHVB7_000283"
     CDS             complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="ACHVB7_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR1912390.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22538..23548
                     /gene="ascG"
                     /locus_tag="ACHVB7_000284"
     CDS             22538..23548
                     /gene="ascG"
                     /locus_tag="ACHVB7_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR1912391.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23696..24223
                     /gene="hydN"
                     /locus_tag="ACHVB7_000285"
     CDS             23696..24223
                     /gene="hydN"
                     /locus_tag="ACHVB7_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR1912392.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            24376..26628
                     /gene="hypF"
                     /locus_tag="ACHVB7_000286"
     CDS             24376..26628
                     /gene="hypF"
                     /locus_tag="ACHVB7_000286"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR1912393.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(26756..27889)
                     /gene="norW"
                     /locus_tag="ACHVB7_000287"
     CDS             complement(26756..27889)
                     /gene="norW"
                     /locus_tag="ACHVB7_000287"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR1912394.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(27886..29325)
                     /gene="norV"
                     /locus_tag="ACHVB7_000288"
     CDS             complement(27886..29325)
                     /gene="norV"
                     /locus_tag="ACHVB7_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR1912395.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29512..31026
                     /gene="norR"
                     /locus_tag="ACHVB7_000289"
     CDS             29512..31026
                     /gene="norR"
                     /locus_tag="ACHVB7_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR1912396.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(31023..31988)
                     /gene="gutQ"
                     /locus_tag="ACHVB7_000290"
     CDS             complement(31023..31988)
                     /gene="gutQ"
                     /locus_tag="ACHVB7_000290"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR1912397.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31981..32754)
                     /gene="srlR"
                     /locus_tag="ACHVB7_000291"
                     /gene_synonym="gutR"
     CDS             complement(31981..32754)
                     /gene="srlR"
                     /locus_tag="ACHVB7_000291"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR1912398.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(32821..33180)
                     /gene="gutM"
                     /locus_tag="ACHVB7_000292"
     CDS             complement(32821..33180)
                     /gene="gutM"
                     /locus_tag="ACHVB7_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR1912399.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(33286..34054)
                     /gene="srlD"
                     /locus_tag="ACHVB7_000293"
                     /pseudo
     CDS             complement(33286..34054)
                     /gene="srlD"
                     /locus_tag="ACHVB7_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            complement(34058..34429)
                     /gene="srlB"
                     /locus_tag="ACHVB7_000294"
                     /gene_synonym="gutB"
     CDS             complement(34058..34429)
                     /gene="srlB"
                     /locus_tag="ACHVB7_000294"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR1912400.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(34440..35399)
                     /gene="srlE"
                     /locus_tag="ACHVB7_000295"
                     /gene_synonym="gutE"
     CDS             complement(34440..35399)
                     /gene="srlE"
                     /locus_tag="ACHVB7_000295"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR1912401.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(35396..35959)
                     /gene="srlA"
                     /locus_tag="ACHVB7_000296"
                     /gene_synonym="gutA"
     CDS             complement(35396..35959)
                     /gene="srlA"
                     /locus_tag="ACHVB7_000296"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR1912402.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFLHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            36215..37300
                     /gene="mltB"
                     /locus_tag="ACHVB7_000297"
     CDS             36215..37300
                     /gene="mltB"
                     /locus_tag="ACHVB7_000297"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR1912403.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37445..37942
                     /gene="pncC"
                     /locus_tag="ACHVB7_000298"
     CDS             37445..37942
                     /gene="pncC"
                     /locus_tag="ACHVB7_000298"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR1912404.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            38022..39083
                     /gene="recA"
                     /locus_tag="ACHVB7_000299"
     CDS             38022..39083
                     /gene="recA"
                     /locus_tag="ACHVB7_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1912405.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            39311..39811
                     /gene="recX"
                     /locus_tag="ACHVB7_000300"
     CDS             39311..39811
                     /gene="recX"
                     /locus_tag="ACHVB7_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR1912406.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            39939..42569
                     /gene="alaS"
                     /locus_tag="ACHVB7_000301"
     CDS             39939..42569
                     /gene="alaS"
                     /locus_tag="ACHVB7_000301"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR1912407.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            42804..42989
                     /gene="csrA"
                     /locus_tag="ACHVB7_000302"
     CDS             42804..42989
                     /gene="csrA"
                     /locus_tag="ACHVB7_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR1912408.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            43305..43397
                     /locus_tag="ACHVB7_000303"
     tRNA            43305..43397
                     /locus_tag="ACHVB7_000303"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43339..43341,aa:Ser,seq:gct)
     gene            43401..43477
                     /locus_tag="ACHVB7_000304"
     tRNA            43401..43477
                     /locus_tag="ACHVB7_000304"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43435..43437,aa:Arg,seq:acg)
     gene            43541..43617
                     /locus_tag="ACHVB7_000305"
     tRNA            43541..43617
                     /locus_tag="ACHVB7_000305"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43575..43577,aa:Arg,seq:acg)
     gene            43816..43892
                     /locus_tag="ACHVB7_000306"
     tRNA            43816..43892
                     /locus_tag="ACHVB7_000306"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43850..43852,aa:Arg,seq:acg)
     gene            44173..44739
                     /gene="yqaB"
                     /locus_tag="ACHVB7_000307"
     CDS             44173..44739
                     /gene="yqaB"
                     /locus_tag="ACHVB7_000307"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR1912409.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            44736..45164
                     /locus_tag="ACHVB7_000308"
     CDS             44736..45164
                     /locus_tag="ACHVB7_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR1912410.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            45237..46793
                     /gene="gshA"
                     /locus_tag="ACHVB7_000309"
     CDS             45237..46793
                     /gene="gshA"
                     /locus_tag="ACHVB7_000309"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR1912411.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            46943..47458
                     /gene="luxS"
                     /locus_tag="ACHVB7_000310"
     CDS             46943..47458
                     /gene="luxS"
                     /locus_tag="ACHVB7_000310"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR1912412.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(47522..49060)
                     /gene="emrB"
                     /locus_tag="ACHVB7_000311"
     CDS             complement(47522..49060)
                     /gene="emrB"
                     /locus_tag="ACHVB7_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR1912413.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(49077..50249)
                     /gene="emrA"
                     /locus_tag="ACHVB7_000312"
     CDS             complement(49077..50249)
                     /gene="emrA"
                     /locus_tag="ACHVB7_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR1912414.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(50376..50906)
                     /gene="emrR"
                     /locus_tag="ACHVB7_000313"
     CDS             complement(50376..50906)
                     /gene="emrR"
                     /locus_tag="ACHVB7_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR1912415.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(50997..51332)
                     /gene="ygaH"
                     /locus_tag="ACHVB7_000314"
     CDS             complement(50997..51332)
                     /gene="ygaH"
                     /locus_tag="ACHVB7_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR1912416.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(51322..52059)
                     /gene="ygaZ"
                     /locus_tag="ACHVB7_000315"
     CDS             complement(51322..52059)
                     /gene="ygaZ"
                     /locus_tag="ACHVB7_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR1912417.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(52183..53367)
                     /locus_tag="ACHVB7_000316"
     CDS             complement(52183..53367)
                     /locus_tag="ACHVB7_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1912418.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(53658..54650)
                     /gene="proX"
                     /locus_tag="ACHVB7_000317"
     CDS             complement(53658..54650)
                     /gene="proX"
                     /locus_tag="ACHVB7_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR1912419.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(54708..55772)
                     /gene="proW"
                     /locus_tag="ACHVB7_000318"
     CDS             complement(54708..55772)
                     /gene="proW"
                     /locus_tag="ACHVB7_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR1912420.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(55765..56967)
                     /gene="proV"
                     /locus_tag="ACHVB7_000319"
     CDS             complement(55765..56967)
                     /gene="proV"
                     /locus_tag="ACHVB7_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR1912421.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(57323..58282)
                     /gene="nrdF"
                     /locus_tag="ACHVB7_000320"
     CDS             complement(57323..58282)
                     /gene="nrdF"
                     /locus_tag="ACHVB7_000320"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1912422.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(58292..60436)
                     /gene="nrdE"
                     /locus_tag="ACHVB7_000321"
     CDS             complement(58292..60436)
                     /gene="nrdE"
                     /locus_tag="ACHVB7_000321"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1912423.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTNEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(60409..60819)
                     /gene="nrdI"
                     /locus_tag="ACHVB7_000322"
                     /pseudo
     CDS             complement(60409..60819)
                     /gene="nrdI"
                     /locus_tag="ACHVB7_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(60816..61061)
                     /gene="nrdH"
                     /locus_tag="ACHVB7_000323"
     CDS             complement(60816..61061)
                     /gene="nrdH"
                     /locus_tag="ACHVB7_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR1912424.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(61309..61638)
                     /locus_tag="ACHVB7_000324"
     CDS             complement(61309..61638)
                     /locus_tag="ACHVB7_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR1912425.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            61790..62134
                     /locus_tag="ACHVB7_000325"
     CDS             61790..62134
                     /locus_tag="ACHVB7_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR1912426.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(62171..62620)
                     /gene="alaE"
                     /locus_tag="ACHVB7_000326"
     CDS             complement(62171..62620)
                     /gene="alaE"
                     /locus_tag="ACHVB7_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR1912427.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT        14829 a        16243 c        16979 g        14952 t
ORIGIN      
        1 atggcatatt ttatgttgtc attaatgatt tcaataatat gtggaaggtg atggaatttc
       61 agcaacagat ctattgcctc ttgttgctct gaatcattta aatcgaaaaa ccagtcacca
      121 ccgctggcaa gccacatatt tccatcgcca gtttcgaatg catccaacgc catcgcttcg
      181 tggttgcctt taaccgacgt aaaccagggt tggtttagca ggcgcagcac gttaagactc
      241 ttcggcccac gatcaatatt atcgcctgtg gaaataagta agtcggtttc agggtaaaaa
      301 gagagttgat gttaacggga ttgtaataac tgatattcac catgaatatc accaacgacc
      361 catatatggc gatagtgatg ggcattgatt ttttgatagc gtgtagatgg catggtttta
      421 ccctgtaaaa taagctttcc tattatacag ggtattttta tttgattcgt cagttgtcgt
      481 taatattccc gatagcaaaa gactatcggg aattgttatt acaccagact cttcaagcga
      541 taaatccact ccagcgcctg acgcggagtc agtgaatccg ggtcgagatt ttccagtgct
      601 tcgaccgcag gcgaagtttc ttctggcact gacagcaaag acatctgcgt accatccact
      661 tgcgtcgcgg cggcgttcgg cgaaatgctt tccagctcac gcagtttttg ccgtgcgcgc
      721 ttaataacct ctttcggtac gcccgccaga gcagcaaccg ccagaccgta gcttttgctc
      781 gccgcgccgt cctgcacgct gtgcataaag gcgatagtgt cgccgtgctc cagcgcatcg
      841 agatgtacgt tggcgacgcc ttccattttc tccggtagct gggtcagctc gaaatagtgg
      901 gtggcaaaca gcgtcaatgc cttaatctta ttcgccagat tttctgcgca cgcccacgcc
      961 agcgacagac catcgtaggt ggacgttcca cgcccgatct catccattaa caccagactg
     1021 tattcggtgg cgttatgtaa aatattggcg gtttcagtca tctccaccat aaaggttgag
     1081 cgcccggaag ccaggtcatc tgccgcgcct acgcgggtaa agatgcgatc gataggtcca
     1141 atctcgactt tttgtgccgg tacatagctg ccgatgtagg ccatcagcgc aatcagtgcg
     1201 gtctggcgca tataggtact tttaccgccc atgttcggac cggtgatgat caacatacgg
     1261 cgctgcggcg acagattcag cgggttagcg ataaatggct cattcagtac ttgttcaact
     1321 accggatggc gaccttcggt aatgcgaatg cccggtttat caatgaaggt cgggcaggtg
     1381 tagttcaggg tataggcccg ttccgccagg ttcaccagca cgtcgagttc cgccagcgcg
     1441 ctcgcgctct gttgcaacgc ttccagatgc ggcaacagca ggtcgaacaa ctcttcataa
     1501 agctgttttt ccagtgccag tgctttgcct tttgaggtga gaactttatc ttcgtactct
     1561 tttagctctg gaatgatgta gcgctcggcg tttttcagcg tctggcgacg catgtagttg
     1621 atgggtgcca gatggctttg cccacggctg atttgaatgt agtagccgtg caccgcatta
     1681 aagccaactt tcagcgtgtc caggccggta cgttcacgct cgcggacttc cagacgctcc
     1741 agataatcgg tcgcgccgtc agccagcgcg cgccactcat ccagctcttc gttatagccc
     1801 gatgcgataa caccaccgtc gcgtaccagc accggcggtg tgtcgatgat tgctcgctcc
     1861 agcagatcgc gcagctcggc aaactcgccc atcttctcac gtagcgcctg taccggtgca
     1921 ctatcgacag tttctaactg cgcacgcagc tccggcagtt gctggaaagc gtggcgcata
     1981 cgggccagat cgcgtgggcg agcagttcgt aaagccagac gtgccagaat acgttccagg
     2041 tcgccgacct gacgcagtac cggctgcaac tcggcggtga aatcctgcaa tgcgccaata
     2101 gtttgctggc gctcaagcaa cacgcgggta tcgcgcactg gcatatgcag ccagcgtttc
     2161 agcatacgac tacccatcgg cgtgacggtg cagtcgagca cagaagccag cgtattttcc
     2221 gcaccgcccg ccaggttctg agtaatttcc aggttacgac gtgtcgcggc atccataatg
     2281 atgctgtcct gctcacgttc catggtgata gaacgaatat gcggcagggt cgtgcgttgg
     2341 gtatctttcg catactgcaa cagacaaccg gcagcacaaa gtccgcgcgg cgcgttctcg
     2401 acgccaaaac cgaccagatc gcgggtgcca aattgcagat tcaactgctg gcgcgcggtg
     2461 tcgatttcaa actcccacag cgggcgacgg cgcaggccgc gacggccttc aatcagcgac
     2521 atctcggcga aatcttctgc atacagcagt tccgccggat tagtgcgttg cagctctgcc
     2581 gccatcgttt cgcggtcggc cggttcgctc aggcgaaaac gcccggagct gatatccagc
     2641 gtcgcgtagc cgaaaccttt gctgtcctgc cagatagccg ccagcaggtt gtcctgacgc
     2701 tcctgtaaca gggcttcatc gctgatggtg cctggcgtaa cgatacgcac aactttgcgc
     2761 tcaaccggac ctttgctggt cgccggatcg ccaatttgtt cgcagatggc aacggactct
     2821 ccctgattca ccagtttggc gagatagttt tccaccgcat ggtagggaat ccccgccatc
     2881 gggatcggct ctcccgccga agcaccgcgt ttggtcagtg aaatatccag cagttgcgac
     2941 gcgcgttttg cgtcgtcata aaacagttca taaaaatcac ccatccggta aaacagcagg
     3001 atctcgggat gctgggcttt cagcctgaga tactgctgca tcatgggcgt atgggcgtcg
     3061 aaattttcta ttgcactcat ggggttatgt ccggttccct gatattaaat ggggtgtgat
     3121 ggtttttatt ttcgttgtaa tcacataagg cgcacatgat accggagtta atcatttcag
     3181 gggaagaagc gaacggcgag ttcttgatgc tggttccggg aaagatgctg ttttgcagct
     3241 tttgcgtgat gcttatgaag caagtacgca aaattgcgtc ggttgaataa acctcctcct
     3301 tcagagccat agtccccttt caaattaagt tgtaatataa atacacctta agagtatgga
     3361 ggcagcatgt ccggcaagcg tatctctcgt gaaaaactga cgattaaaaa aatgatcgat
     3421 ctttatcaag cgaaatgccc gcaggcgtca gcggagccgg agcattacga ggcgttgttt
     3481 gtttacgcgc aaaagcggct ggataaatgt gtgttcggcg aagagaaacc agcctgtaag
     3541 cagtgtccgg tgcactgtta ccagcctgca aagcgtgaag agatgaaaca gattatgcgt
     3601 tgggccgggc cgagaatgct gtggcgtcat ccgatcttaa ccgtgcgtca tctcatcgat
     3661 gacaagcgtc cggtaccaga actgccggaa aaatatcgtc cgaaaaaacc tcgtgaatag
     3721 tatgccggac aggcagatct gtccggcaat ttgcagttaa atcaatgccg atttatcaat
     3781 tcccagccgc ttcatccgtg acagcagggt cgtgcgtttc agccccagac gttgcgcagc
     3841 gcctttaggc ccggcaacca cgccgttggt ttctttcagc acgcgcacaa tcaactgata
     3901 ttcatcttcg ccctcctggg cgacaaccgt tgcggcaggc ggcgtttcag gttccggtaa
     3961 aacaatatct ggcaatgaca gctgcagcac gttaccgcgt gttagcaata ccgcgcgctc
     4021 aatgacgttt tccagttcgc gtacgttacc cggccactcc atgttgctca aggtgcgcag
     4081 cgtctcggca ggaatgctgt cgatattgcg ccccagacga cgggcaattt tgaaggtaaa
     4141 ggctttcgcc agcagcggaa tatcttccgg acgctcgcgt agtggcggca ggtgaatcgg
     4201 gaatacgttc aggcggtaat agagatcgct acggaactct cggtcggcga ccattttttt
     4261 cagatcgcgg ttagtcgcgg caattaaacg tacgtccgtc tgaatgattt tgttgctgcc
     4321 gagacgttca aactcctgtt cctgcaatac acgcagcaac ttcggctgta actccagtgg
     4381 catatcgccc acttcgtcga ggaacagaga gcttttatcc gccagttcaa aacgaccaat
     4441 gcgctgggcg ctggcaccgg taaaagcccc acgctcatga ccaaacagat cgctttccag
     4501 caatccggca ggcatcgccg cgcagttcat tttgaccatg cggcgattat tacgcccact
     4561 gagattatgg atcgcgcggg caatcagctc tttacccgtg ccagtttcac cgaggatcag
     4621 cacggtactg tcactttgcg ccaccatttc aacttgttta agcacgctgt acatggcttc
     4681 gctgcggcca ataatctcgc caaattcact atcaacattg ttgagctgct cggtcagggc
     4741 gaggttttca tcaaccagcc gttctttcag acgatggatt tcctgatagg cgagggcgtt
     4801 atcgacagcg attgccacac gttcggcaat ctggcgcagc aaattcagat tggtagtggt
     4861 aaacactttc tcttcgcatt gcgccagttt cagcacgccc agcatggtgt cgccagacat
     4921 cagcggtaac aggcacaagg tttgaatctg gttgccctag gtgtcgaaca acatgcgttc
     4981 atagggggct aaatcgtccc gttcgtggag attgatcagc agcatctctt tacttttgaa
     5041 cacgcgttcg gtgagggttc cggcttcatc gacttcgctc tgttcgtggg cgggatgttg
     5101 tttatcaaga tagtgagtgg agtagatgtt gagtttgttt ttacggtggc tgcgtaagac
     5161 gatactgata tcgtcaatgt cgaaatagta atggatttct ttggcgactt cgctgaccag
     5221 ttcgtccata tccaggcggg aaagcaccgc gttggtaatg gcaaccagga tgcggaagtt
     5281 atcgcgctcc cggcataaca actcatagtc gacattgttg acgacgcggc tctggatttg
     5341 ttcggtgacg acagaaacga tctgcgtaaa tgtttgcaga cgattgaact ctttttcgct
     5401 ccagggacga tcgtcataac gaataaattc acagccacca aaaatatgcc cttccgccgc
     5461 cagtggcatc aggcaatagt gaccaaattt ggggtatagc ccacctgcga ccagctgcgg
     5521 ccaggtttca caaaattctt cgtaactgca atgcagcgta tcagggcgcg acaaaatgcg
     5581 gcgtaccgga ccgtgtgcca gaacagtttc gtcttcatat ttaatggggt gtctttttca
     5641 cgcgatgcgt aataagacgc acgttgagtc tgcgcttgcc acaacacaat agccgcgttg
     5701 tcggcgagcg cagaacgctt taccagttgc gaaagagcct cacacagcga ggccagatcg
     5761 ggctgctgca atagtgtccg agtgatgtcg aacaaccctt gctgtccgag atcactcatc
     5821 ggtgtatatg acattttgtt cttccaggaa agcaccgcaa agcggtgcaa aaggtcattg
     5881 actaactata ggagatttag aattttatta gcatatacgc ggaagaggtt cggcgtgtgg
     5941 taaatcgagg gttcgtttca caccatacag accggcaaga cgaacacctt tacgttccac
     6001 cacttcacca attagcgccg cgtctttccc cagtggatgg gaatgtaacg ctgccagcac
     6061 ttgctctgcc gcgttgcgtt caacggcgat caccagtttg ccttcgttgg caaagttaag
     6121 ggcgtccagt cccagcagtt cgcaaacgcc gcgcacggca ggtttaaccg gcagtgccac
     6181 ttctgaaatt tcaataccac aaccgcaggc tgccgcgaac tcatgaacta ccgcgttcac
     6241 accaccacgg gtggcatcac gcagcgcttt cacgccggga atgtcacgca gcgtctgaat
     6301 aagcggcgtc agtaccgcgc agtcgctgac cagttcgcca tccagcccca gctgctcacg
     6361 caggttaagg atagtcgccc cgtggtcccc gagcgtgccg ctaaccagta atacatcgcc
     6421 tgcggttagc gtctgtgcgc cccagtgaat attcgccgga attgcgccca tgccagcggt
     6481 gttgataaac agtttatctg ccgcgccgcg ctgcaccact ttagtatcgc cagtaacgat
     6541 ggcaatgcct gccgcgcgcg cggtttctgc catgctggtc actacggctt tcagtgtctc
     6601 catcggcaat ccttcttcga ggataaagcc acaggagaga tagcgcggaa tagcgccact
     6661 gaccgcaacg tcattggctg tgccgcaaat cgccagcttg ccgatattac cgccaggaaa
     6721 gaacagcggg tcaatgacgt aactgtcggt ggagaacgcc agacggtcgc cttctgctac
     6781 cagctgcgcc agatcaagac gtgcctggtc ttcctgctct gccagccacg ggttggcaaa
     6841 ggcttccata aacaggctgt tgattaattg ctgcatcgcc tggccgccgc tgccgtgggc
     6901 gagttggata ttattcacgc ttcattctcc tgctgacgat actgatacca cgcggcgcac
     6961 gctccttcgg aggagaccat cagtgcacca aatgcggttt gaggattaca ggtgttacca
     7021 aacagcgggc attgatgcgg cttacatttg cccgttaata cctcaccaca acgcgcgcgc
     7081 gggtcatcgc agacctgctg cggtgctggg cggaaatgtg cttcggcatc gaatcgttga
     7141 taatccggcg tcaggtgcac gccagaagat tcaatcacgc ctaagccgcg ccattcgctg
     7201 tcgccgttga cacagaacac atcagcaatt gcctgttgcg ccagcaggtt accggcatcc
     7261 ggtaccaccc gacgatactg attctctacc ttgctgtggg ccgctatttt ctgctccacc
     7321 agcatgacca cgccttgcag tagatcaagg ggttcgaaac cagcgaccac cagcggacga
     7381 tgaaaatcgc tggcgataaa attataggca tcagtgccga taaccatact aacgtggccc
     7441 ggcgcgagga acgcgtcgat accgttatcc ggctgttcca gcaaactgcg cagcgtcggg
     7501 ataagcgtaa tatgctggca gaagaagtaa aaattctgca catcgcgggc tttcgcctgt
     7561 tgcagggtga tggcggtggt cggcatggtg gtttcaaaac ctaagccgaa gaatacgact
     7621 ttacgggttg gattctcctg cgccagtttc aacgcatcca tcggcgagta aacgatgcgc
     7681 acatcggcac cgcgtgcttt tgcctgcaac agcgatccct gtttccccgg cacgcgcatg
     7741 gcgtcgccaa aggtacagaa gatgacttcc ggatggctgg caatctccac gcaggtgtcg
     7801 attctaccca tcggcagtac gcacaccggg caacccggac cgtggataaa ctcaacgttt
     7861 tcaggcagta actggtcgag gccgaattta aaaatggcgt gggtatgacc accgcacact
     7921 tccataatcc gcagagggcg ttcggcggtg taagagagat gtgaagcacg ttcgcgcaga
     7981 tgctcaatta actgcatcac ctgttccggc gcgcgatatt catcaacaaa acgcattatt
     8041 tttcctcgcc atacaacagc gcgccgacat ccggctcaac gtcaaacatg ttttgtaagg
     8101 cgtcgagagt gtcgcgtgct tcggcttcat taattacgct catggcaaag ccaacgtgta
     8161 ccagtaccca ctggcccacg cgcggctgac cgttttcatc gcagctgccg actaacgtta
     8221 aatcgacatc gcgctgaatg ccgcagacgt cgacttttgc ctggttgccg tcaatggtgc
     8281 ggatctggcc gggaacgcct atgcacatcg ctgtgtctcc agccagttca gccactggtc
     8341 catcccttcg ccgctggtgg cggaaataag gatgatttca atttctggat tgacttcgcg
     8401 ggcgcaggcg atgcacttct caacgtcaaa gttgagatac ggcaacaggt caactttgtt
     8461 gagcagcatc agcgaggcgg cggcaaacat atgcggatat ttcagcggtt tgtcttcacc
     8521 ttcggtaacg gaaagcaccg ccactttgtg tttttcaccg agatcgaagc tggccgggca
     8581 tacgaggttg ccaacgtttt cgataaacag aataccgtta tcgtccagtg gcagacgcgg
     8641 tgcggcgtcg gcaatcatct gtgcgtcaag atggcagcct ttaccggtgt tcacctgaat
     8701 cgctggtgtg ccggtagcgc gaatgcgtgc ggcatcgttc acggtttgct ggtcaccttc
     8761 aataaccgcg cacggaacgc tgtctttcag gcgcattagg gtttccgtca gcagggtggt
     8821 tttaccggaa ccagggctgg aaaccaggtt gagcaccagt tgcttgcggg cagcaaagcg
     8881 cgcgcggttg cgttcagcca gacggttatt tttgtccagc acgtcaattt cgacttccag
     8941 catccgacgc tggctcatgc ccggcgcgtg agtgcccgct tcgccatgac cgtaatgcag
     9001 gtcgccttct tcagtctggc tgggggtaaa ttcaggcgct ttaatgccgg tgattttcat
     9061 cttcgggcgt gccgccgggg caaatggcgc gctacgaaac gcggaatgag ggttatgttc
     9121 atcaccctcg atatacaggt tgccttcacc gcaaccgcat gttgtacaca tcgctcactc
     9181 ctggtctatt tctatccgcc gaatctgtaa accgtcgtct gccacaatct gtagcatgtc
     9241 accatgacac tgtggacagc ggcggacgcg ctgggtcagt agcgtcacat actgttggca
     9301 tgtttcacac cagcattcgg cctcttgttc ttcgaggtgc agtttacaac cttccgccac
     9361 gctgccgcgg caaaccagat caaaacaaaa ggcaagagag ctggtttcga cacaagaaaa
     9421 tgcgccaatt ttgagccaga ccccagttac gcgttttgcg ccgtgttttg cggcctgctg
     9481 ttcgatcaat tccagtgccc gttggcagag ggttatttcg tgcatatcgc ctcccattaa
     9541 ctattgccag ctacaagcaa taattgtgcc agtgttgatt atccctgcgg tgaataatgt
     9601 cgatgatgtc gaaatgacac gtcgacacgg cgacgaaatt catctttagc ttaaaaatct
     9661 atttaataac aataaattaa aagttggcat agaaaatgct taaagctggc atctctgtta
     9721 aacgggtaac ctgacaatga ctatttggga aataagcgag aaagccgatt acatcgcaca
     9781 gcggcattgt cgcctacagg accagtggca catctactgc aattcgctgg ttcaggggat
     9841 cacgttatcg aaagcgcgcc tgcatcacgc catgagctgc gcgccggaca aagaactctg
     9901 tttcgtcctt tttgaacatt ttcgtattta cgtcaccctg gcggatggct ttaacagcca
     9961 caccatcgag tattacgtcg aaacaaaaga tggcgaagac aaacagcgga ttgcgcaggc
    10021 gcaactgagc attgacggca tgattgatgg caaggtcaac atccgcgatc gcgaacaggt
    10081 tctggaacac tatctcgaaa aaatcgctgg cgtttacgac agcttataca ccgctattga
    10141 aaacaatgtg ccggtgaatt taagccaact ggtaaaggga caaagcccgg cagcatgagc
    10201 tgaggctttg cccgttttgc aggcgttacg cctgtttggg gatgggcgtg tcgatgagtg
    10261 tcgaaaatga catttcatcg gcatgttttc gtcaaaaatg acaatcacct gaggaatgcc
    10321 tggtgaatcg ttttgtaatt gttgactcca cgctctgtat cggctgccac acttgtgagg
    10381 ccgcctgttc agagacgcat cgccagcacg gcctgcaatc aatgccgcgc ctgagagtga
    10441 tgctgaatga aaaagaatct gcgccgcagc tctgtcacca ctgtgaagat gcaccctgtg
    10501 cggtggtttg cccggttaac gccatcaccc gcattgatgg ggccgtgcag ttgaatgaaa
    10561 gtctgtgcgt aagctgcaaa ctgtgcggca tcgcctgccc gtttggcgca attgaatttt
    10621 ccggcagccg tccgctggat attccggcaa acgccaatac cccgaaagcg ccaccggcac
    10681 cgcctgctcc ggcgcgtatc agtacgctgc ttgactgggt gccgggtatt cgcgcgatcg
    10741 ccgtcaaatg tgacctttgc agttttgatg aacaaggtcc ggcctgcgtg cggatgtgcc
    10801 cgactaaagc cctgcatctg gtggataaca ccgatatcgc ccgcgtcagc aaacgtaagc
    10861 gtgagctgac ctttaacacg gactttggcg atctcacctt gtttcagcag gctcaaagtg
    10921 gagaggctaa atgagcgcaa tttccctgat caatagcggc gtggcatggt ttgtcgccgc
    10981 cgctgttctg gcatttctct tttcttttca aaaagcgtta agtggctgga tagctggaat
    11041 tggcggcgcg gttggtagtc tgtatacggc agccgcgggc ttcactgtac tgactggcgc
    11101 ggttggcgtg agcggtgcgc tgtcgctggt aagctacgat gtgcaaatct ctccgcttaa
    11161 cgcgatttgg ctgattacgc tcggtctgtg cggtctgttt gtcagcctct acaacattga
    11221 ctggcatcgc cacgcgcagg tgaagtgcaa cggcttgcag atcaatatgt tgatggctgc
    11281 cgccgtctgc gccgtcatcg ccagcaacct cggcatgttc gtggtaatgg ccgaagtcat
    11341 ggccttgtgt gcggtgttcc tcaccagcaa cagcaaagag ggcaaactgt ggtttgcgct
    11401 ggggcgtctt ggcactctgc tgctggtgat tgcttgctgg ctgctgtggc agcgttatgg
    11461 cacgctggat ctgtgcctac tggatatgcg tatgcaacag ctgccgctcg gttccgatat
    11521 ctggctgctc ggtgtgattg gctttggcct gctggccggg attattccgc tgcacggctg
    11581 ggtgccgcag gcacatgcta acgcctctgc gccagctgcc gcgttgtttt ctacggtagt
    11641 catgaaaatt ggcctgctgg gcatgttaac cctgtcactg ctgggcggta atgcaccgct
    11701 gtggtggggg atcgcgctgc tggtgctcgg catgatcacc gcgttcgtcg gtggtctgta
    11761 tgcgttgatg gaacacaaca tccagcgcct gctggcttac cacaccctgg aaaatatcgg
    11821 cattatcctg ctggggctgg gcgctggcgt aacgggtatc gcgctcgaac aaccggcgct
    11881 gattgctctt ggcctggtcg gtggtctgta ccatctgctt aaccatagcc tgttcaaaag
    11941 cgtactgttc ctcggggcgg ggagcgtctg gttccgtacc ggtcatcgcg atatcgaaaa
    12001 actcggtggt attggcaaga aaatgccggt tatctccatc gccatgttag tcgggttgat
    12061 ggcaatggct gcgctgccgc cactgaacgg gtttgccggg gaatgggtta tctatcaatc
    12121 cttcttcaaa ctgagcaata gcggcgcgtt tgttgcccgt ctgctggggc cgctgctcgc
    12181 tgtgggtctg gcaattaccg gtgcgctggc ggtgatgtgt atggcgaaag tctatggcgt
    12241 cacgttcctc ggcgcgccgc gtaccaaaga agccgaaaac gccacctgtg cgccgctcct
    12301 gatgagcgta agcgtagtgg cactggcgat ttgctgcgta attggcggtg ttgctgcgcc
    12361 gtggctgctg ccgatgctct ctgctgctgt acctctgcct ctggagcctg ctaacaccac
    12421 cgtttctcaa ccaatgatca cattgctgct gattgcctgc ccgctgctgc cattcatcat
    12481 tatggcgatt tgcaaaggcg accgcctgcc gtcacgttcc cgcggtgcgg cctgggtgtg
    12541 cgactacgac catgaaaaat caatggtgat taccgctcac ggttttgcca tgccggttaa
    12601 acaggcgttt gcgccagtgc tgaaactgcg caaatggctg aatccggtgt cgctggtgcc
    12661 gggctggcag tgcgaaggta gtgcgttgct gttccgccgg atggcgctgg ttgaactggc
    12721 ggtactggtg gtgattattg tttcacgagg agcctgagaa tgagtgtttt atatccgtta
    12781 attcaggcgc tggtgttatt tgccgttgcg ccgctgctct ccggtattac ccgcgtggcg
    12841 cgcgcccgtc tgcataaccg tcgtgggccg ggtgtgttgc aggagtatcg tgacattttc
    12901 aaactgctgg gccgccagag cgttggcccg gatgcctccg gctgggtgtt ccgcctgacg
    12961 ccgtatgtga tggtgggcgt catgctgact atcgctactg cgctgccggt ggtgaccgtc
    13021 ggttctccgc tgccgcaact gggtgattta atcaccttac tgtatctctt tgccatcgcg
    13081 cgtttcttct ttgccatttc tggtctggat accggtagcc cgtttaccgc tatcggcgcg
    13141 agccgtgaag cgatgcttgg cgtgctggtc gaaccgatgc tgctgcttgg tctgtgggtt
    13201 gccgcacagg ttgccggttc caccaacatc agcaacatca ccgacaccgt ttatcactgg
    13261 ccgctgagcc agagcatccc gctggtactg gcgctttgtg cctgtgcgtt cgccaccttt
    13321 atcgaaatgg gcaaactgcc gttcgacctg gcggaagccg agcaggagct gcaggaaggc
    13381 ccgctctctg aatacagcgg cagcggcttt ggcgtcatga aatggggtat cagcctgaaa
    13441 cagctggtgg tgctgcagat gttcgtcggc gtatttatcc cgtggggaca gatggaaacc
    13501 ttcaccgccg gtgggctgct gctggcgctg gtgattgcca tcgtcaaact ggtcgtcggc
    13561 gtcctggtta tcgcgctgtt cgaaaacagc atggcccgtc tgcgtcttga tattactccg
    13621 cgcattacct ggactgggtt tggctttgca tttttagcgt tcgtctcctt gctggcggcg
    13681 tgattaaaga gagtttgagc atgtctgaag aaaaattagg tcaacattat ctcgccgcgc
    13741 tgaatgaggc atttccgggc gtcgtgctgg accacgcctg gcagaccaaa gatcagctga
    13801 ctgtcaccgt aaaggtgaac tatctgccgg aagtggtgga gtttctctac tacaagcagg
    13861 ggggctggct gtcggtgctt tttggtaacg acgaacgcaa actgaatggt cattacgccg
    13921 tttattacgt gctgtcgatg gagaagggga ctaagtgctg ggtaacggtt cgcgtcgaag
    13981 ttgatgccaa caaaccggag tatccgtccg tgacgccgcg cgttccggcg gcggtgtggg
    14041 gcgagcgcga agtgcgcgat atgtacggtt tgattccggt tggtctgccg gatgaacgcc
    14101 gtctggtgct gccggatgaa ctttatccgc tgcgtaaaga cagcatggat tatcgtcagc
    14161 gtccggcacc gaccaccgat gctgaaacct acgagttcat caacgaactg ggcgacaaga
    14221 aaaacaacgt cgtgccgatt ggtccgctgc acgtcacttc tgacgaaccg ggccacttcc
    14281 gtctgttcgt cgatggcgaa aacattatcg acgccgacta ccgtctgttc tacgttcatc
    14341 gcggcatgga aaaactggcg gaaacccgta tgggttacaa cgaagtgacc ttcctctctg
    14401 accgtgtgtg cgggatctgc ggctttgccc acagcaccgc ctacaccacg tcggtagaaa
    14461 acgcgatggg tattcaggtg ccagaacgtg cgcagatgat ccgcgccatt ctgctggagg
    14521 tggaacgcct gcactcgcat ctgctcaacc tcggtctcgc ctgccacttt accggctttg
    14581 actccggctt tatgcagttc ttccgcgtgc gtgaaacctc catgaaaatg gcagagatcc
    14641 tcaccggtgc gcgtaaaacc tacggcctga acctgatcgg cgggattcgt cgcgatctgt
    14701 tgaaagatga catgatccag acccgccagc tggcgcaaca gatgcgtcgt gaagtgcagg
    14761 agctggtgga tgtgctgctg agtacgccga acatggaaca gcgcaccgtc ggcattggtc
    14821 gtctggaccc ggaaatggcc cgcgatttca gtaacgttgg cccgatggtt cgcgccagcg
    14881 gtcacgcccg cgatactcgc gccgatcacc cgtttgtcgg ttatggcctg ctgccaatgg
    14941 aagtccacag cgagcagggc tgcgacgtta tttcccgtct gaaagtgcgt atcaacgaag
    15001 tttataccgc gctgaacatg atcgactacg gtctggataa cctgccgggc ggtccgctga
    15061 tggtggaagg ctttacctac attccgcacc gtttcgcgct gggctttgcc gaagcgccgc
    15121 gcggcgacga tatccactgg agcatgaccg gcgacaacca gaagctgtac cgctggcgct
    15181 gccgtgccgc gacctacgcg aactggccga ccctgcgcta catgctgcgc ggtaacaccg
    15241 tttccgatgc gccgctgatt atcggtagct tggacccttg ctactcctgt accgaccgca
    15301 tgaccgtggt cgatgtgcgt aagaaaaaga gcaaagtggt gccgtacaaa gaactcgagc
    15361 gttacagcat tgagcgtaaa aactcgccgc tgaaataagg aatcgccatg tttaccttta
    15421 tcaaaaaagt catcaaaacc ggcacggcga cctcgtctta tccgctggag ccgattgcgg
    15481 ttgataaaaa cttccgtggt aagccggagc agaacccgca gcagtgcatc ggctgtgcgg
    15541 cctgcgtcaa tgcctgcccg tcaaacgcct taacggttga aactgacctc gccaccggtg
    15601 agctggcgtg ggagtttaat ctcgggcgct gcatcttctg tggacgctgc gaagaagttt
    15661 gcccgacggc ggcgatcaaa ctgtcgcaag agtacgaact ggcagtgtgg aagaaagaag
    15721 acttcctgca acagtcccgc ttcgcgctgt gcaactgccg cgtctgcaat cgtcctttcg
    15781 ccgtccagaa agagatcgac tacgccattg cgctgcttaa gcacaacggc gacagccgcg
    15841 cggaaaacca ccgcgaaagc tttgagactt gcccggaatg taagcgccag aaatgcctgg
    15901 tgccgtccga ccgtattgaa ctgactcgcc atatgaaaga ggccatctga tgagcaattt
    15961 attaggcccc cgtgacgcca acggcattcc ggtcccgatg acggtggatg aatccatcgc
    16021 cagcatgaag gcgtcgttac tgaaaaaaat caaacgttct gcctacgttt atcgcgtgga
    16081 ctgcggcggc tgcaacggct gcgaaatcga aattttcgcc acgctttcgc cgctgtttga
    16141 tgcagaacgc ttcggcatta aagtcgttcc ttcaccgcgt catgcggata ttttactgtt
    16201 taccggcgcg gtcacccgtg caatgcgatc ccctgcgctg cgtgcgtggc agtccgcgcc
    16261 ggacccgaaa atttgtatct cctacggtgc ctgcggtaac agcggtggta ttttccacga
    16321 tctctactgc gtgtggggcg gtacggataa aatcgtcccg gtagatgtct acattcctgg
    16381 ttgcccgcca acgcctgccg ccacgctgta cggctttgca atggcgctcg gcctgctgga
    16441 gcagaaaatt cacgcccgtg gcccgggtga gcaggatgaa caacaggcgg aaatcctgca
    16501 tggcgatatg gtgcagccgc tgcgtgtgaa agtggatcgc gaagcacgcc gcctggcggg
    16561 ttatcgttac ggtcgtcaga ttgccgatga cttcctgaca cagttagggc agggcgaaga
    16621 acaggttgca cgctggctgg aagcggaaaa cgatccgcgt ctgaacgaga ttgtcagcca
    16681 tctgaatcat gttgttgaag aggcgcgtat ccgatgagtg aaaaggtggt gttcagtcaa
    16741 ctgagccgta aatttattga tgagaacgat gccacgcccg ccgaggcgca gcaggtggtc
    16801 tattacagcc tggcgattgg tcaccacctt ggggttatcg attgcctgga agcggcgctc
    16861 acctgcccgt gggatgaata tctggcatgg attgccactc tggaggcggg cagtgaagcc
    16921 cgccgcaaaa tggaaggcgt gccgaaatat ggtgagatcg tcatcgacat taaccatgtg
    16981 ccgatgctgg ccaacgcatt cgataaagcc cgggcagcgc aaacttcgca gcagaaggaa
    17041 tggagtacaa tgctgttaag tatgctgcat gatattcatc aggaaaacgc catctatttg
    17101 atggtgagga gactgcgtga ctgacgtttt actctgtgtt ggcaatagca tgatgggcga
    17161 tgatggcgct ggcccgctgc tggcggaaaa gtgcgccgcc gcgccgaaag gtaactgggt
    17221 ggtgattgat ggcggtagtg caccggaaaa cgacatcgtc gccatccgtg aactgcgccc
    17281 tacgcggtta ctgattgttg acgctactga tatggggcta aaccccggcg aaatccgcat
    17341 catcgacccg gatgatatcg ccgagatgtt tatgatgact acccataaca tgccgctgaa
    17401 ctatcttatc gaccagttga aagaagacat tggcgaagtg attttcctcg gcatccagcc
    17461 ggatatcgtc ggcttttact acccgatgac tcagccgatt aaagatgcgg tagaaaccgt
    17521 ttatcaacga ctggaaggct gggaagggaa tggcggcttc gcgcagttag cggtggaaga
    17581 agaataattc tccatcaggg aattcagcga aatgaatatc agaacaggga tgtgcgcttt
    17641 tttattgagc ttagtgctgc ctgcacaggc caccagcttt accgaatatc tgccaatgag
    17701 tgacagcgaa tacgcgcaaa agcgggcgct gaaacctctc ctgaccatgc cttatgatgc
    17761 cgagcaaacc tggcatttta gaaaagtggg cgttgctggc gtgacgcttg aaaagatgcc
    17821 aaatgatgat agtgaatggc aattgaacgg taaagatcgc gccggtaaat cctggtctgt
    17881 gcctgtgggt atgctggcaa acatagcggg caaagggcaa ctctatcgcg ccgatcttga
    17941 tcgtaacggc attcaggatc tggttatctg gttaccaagt accggtaatg gtctggctcc
    18001 ttatgcacat ttaattctta tgacgttcac gcgcgaaggc cgtccctgtg tctttgaacc
    18061 caggggattc tacaccgcaa gcaaaactgg cgtagatgat ttgctggatt tgcaaggcaa
    18121 tggtcacacg caattgctgg atatgcagtt tgattccggt tactggataa ccagtctgta
    18181 tcaggtaaaa gacgccaaat ggcagcgtgt tcacggctgg tttggcaagt taagctatcc
    18241 cgcgctcacg cgttttactt atacccccaa ccgtaaactc gtcctcaaac ccatcgccgg
    18301 gcgcgacccg caaacggaag atctggcgca gacgcagcgt tgcttgataa agggggatgt
    18361 tcttgatggg gtgaaccaga actgactttt tgcagagtgg ctatgaggat tttgggggta
    18421 attatatcgc gctcaagtgt ttacatattg tgaacttgga tttgatgagc tatagtttcc
    18481 gaacacataa ggatagaact cgcagatgtg gctgtttact tactataagg aagttaaaac
    18541 ttaaaaatgc acatcatttc taaagcgcca tttgaagagt ctgcgcgtaa atatcctaac
    18601 gacgctctgg ctcttcaggc attgtatcgg gttattaagg aaacggattt ttctacgcca
    18661 gaagagatgc gaacggcgtt tcccaacctc gacaatttta aatatcgcaa caaatggtac
    18721 gttctggatg taggtgggaa caacctaagg gtgattgcct acataaactt tgttaataag
    18781 cggttctttg tgaagcacat aactaatcac gccgagtatg acaaactgac acgttactac
    18841 agagagaaca aagaatgact gctaatgctg cacgcgctgt taaagcaaca agagaactgg
    18901 tcaatgcagt cccctttctg ggaggtagtg actcagaaga tgattatcgt gaagcactgg
    18961 aactggtcga atatttgatt gaagaagatg acaccaatcc acttatcgac tttctggcga
    19021 gccgcattgc agaatatgaa aacaacaacc aagagtttgc agagttcgac aaagccgttg
    19081 cggcaatgcc tgttggcgtg gcgttacttc gcacgctgat tgatcaacat aatctgacct
    19141 atgcggattt gaagaacgaa atcggctcta aatccctggt cagtcagatt ttgtctggac
    19201 aacgatccct gacgatatcc cacattaagg cactttcagc ccgatttggc gttaagccgg
    19261 aatggtttct ttgattttga ctgatgccaa accccagatt gcccgatgcg ctgcgcttat
    19321 cgggccttcc tggttcgtgc gacatgtagg ccggataagg cgttcacgcc gcatccggca
    19381 ctgttaccta ctctaaatct tccccattac tggcaatcac ttttttatac caccagaacg
    19441 atttcttacg tgtgcgcgtc agcgtgccgt tgcctgcgtc gtcacggtct acgtagacaa
    19501 aaccgtagcg tttgctcatt tcacccgtag aggcggagac taaatcaata cagccccagg
    19561 tggtgtagcc catcagcgga atgccatctg caatcgcttc gcccattgcg cggatatgtt
    19621 cgcgtaaata gctgatgcga tagtcatcgt taatctcgcc attggcagca agttcatctt
    19681 ttgcgccgag gccgttttcc accagaaaca gcggcttctg ataacggtcg tacatcatgt
    19741 tcatggtgat acgcagaccg agtggatcaa taccccagcc ccagtcgctc acttgcagat
    19801 acgggttacg cagcgatttc actacgttcg ccgcactgct attgttggcg ttcatctccg
    19861 ccgaggcgca gcgcgaggcg taatagctga aagagacaaa atcgacggtg tttttcagga
    19921 tttcatcatc gcccggtgct ttgtcgatgg ttaccccttt ttcgcggaat acgcgggcag
    19981 agtaagccgg atacgtgccc cgcgcctgca catcgataaa aaacaggttt tcccggtctt
    20041 tctccagcgc cgcccagaca tcttccggct tgcaactgta agggtagaag ttaccgcccg
    20101 ccagcataca gcccacctga ttttgcgggt taacctcatg ggcgattttg gtggctagcg
    20161 cactggcaac cagctggtga tgcgcggcct gatatttcac ctgatcctga ttttcacctt
    20221 cttcaaacac cagacccgcg ccggagaacg ggctatgcaa cataatgttg atttcattga
    20281 aggtaagcca gtatttcacc agaccatcaa atgcttcaaa gcaggtccgg gcgtagcggc
    20341 tgaaaaactc caccagcttg cggttacgcc aggagccata ttcggtgacc agatgcatcg
    20401 gcacatcgaa gtggcacaac gtgaccagcg gttcgatacc gtacttttta cactcttcaa
    20461 agacagaacg ataaaaagca atgccctgct gattgggcgt gatttcatcg ccctgcggaa
    20521 agagacggct ccaggcaatt gaggtacgga aaaccttgaa tcccatctct gccatcaggg
    20581 cgatatcttc tttataacga tgataaaaat ccgtcgcctc atggctggga taaaactcgt
    20641 catcgcgcaa ctgaaaacgt ttttccagcc ccagtttcac cgccattcga tgctcgccgt
    20701 gtgggatcat atcgacagtg gtcagccctt tgccaccttc acggaacgca ccttcagact
    20761 ggttggcggc aagcgcgccg ccccataaaa aaccttctgg aaatactgac attttcatcc
    20821 tcaattaaga cttacttctt tggcgacggt cggttgtacg ctctgatgct ttcgcgcctc
    20881 ggcagccgct tcctcaacag gaatatcctc aaagccgagc aacagtgtga ggataaacga
    20941 cagcaccacc gccagcgcca tgaccgcgaa cacccagacg atgctcattg gattcgccgg
    21001 atcgaagaat tgcacgctgg taaataaccc tggcgctgcc attgagtggc tggcaagccc
    21061 cgccataccg gcaaccgcgc cgcaaataaa accgctgata agactggcga taagcggacg
    21121 tttcaggcgg atcgccacgc cgtataacgc cggttcggaa atccccgcca taatggctga
    21181 tgccgccgca gccagcgccg tctggcgcag ttccgggttt ttcgttttcc acgccaccgc
    21241 cagtgatgaa ccgcccagcg acaggttagc gccgatctct gacggcatga ccatcccttc
    21301 tttgccggtt tcggcaatgg tctgaatgat ggttggcgta aagacgcggt gcatcccggt
    21361 cattaccagc agaggccaca gcgcgcccat aatggcgact gaaagccagc ccagataacc
    21421 atgaatggtg taaaccagcg ccgaaatggc gctaccgatc cagataccaa tcgggccaat
    21481 cagcaggatt gccagcggtg cggcaatcaa cacaatcagc attggcttga ggaagttttt
    21541 ggtcaccgcc ggtgtaatgc gatccaccca gcgttcgata tatgacaggc accaggtcat
    21601 caccagcgcc gggatcactg tgtaggtgta tttcaccgcg gtgaccggaa tcagggcaaa
    21661 ttcgacatgt tcaccctggg ccgctttcgc catcagttca ataaaactcg gatgtaccag
    21721 cacacccgcg atggcaatcg ccagcgacat attggtttta aatttgatgg cggcagatgc
    21781 cgcgaccatc agcggcagga agaagaaagc accgtcacca atcacattca gaatggttaa
    21841 ggtcggcgat ccttttgtca gcacgccgct catctcgagg atcatcgcca gcagtttgac
    21901 catcgatccg ccgataatcg ccgggatcag cggtgacatg gtgccgatca gcgcatcgag
    21961 gatccccgca ccaatgcgac gcagcgtgag tttgggttta cccacgggct gtgcgggctg
    22021 catatctccc ggcagcaggc tgacgacttc ctgaaaggct tgtgaaacgg tattgccgat
    22081 aatcacctga cactggttgt cactacgtac cacgccgagc acgccgggga tggtttttaa
    22141 cgtcgggctg tcgataagct ggtcatcttt gataacaacg cgcaaacgcg tcatacagtg
    22201 cgtgaccgcc gagatgttat caacgccgcc cagtgccgct atcaccgagc gtgccagcgc
    22261 cgcataattt ttggccatcg gattttatcc tgttatcagt agggtagatg tagttgttat
    22321 cagtcgacgt aacggaaaaa tgccgatgct gaaacggaat taggaaaccg gtttcacaaa
    22381 atcatgaata gttgtcacta aataagcaag agagttaatt gttcattttt tatatttgtg
    22441 aaaccggtca cctgaatatt tttcccgccg attgcgcaag gacaatcggc aaacggtgca
    22501 gtacactgcg ttgattgcgt gtaaagggat gaaaatgatg acgacgatgc tggaagtggc
    22561 gaagcgcgcc ggggtttcaa aagcgaccgt ttcccgcgtg ctttcaggta atggctacgt
    22621 cagccaggag actaaagatc gcgtgtttca ggcggtagaa gagagcggtt accgcccaaa
    22681 cttgctggcg cgcaatctgt cggcgaagag tactcagacg ctggggttgg tagtgaccaa
    22741 cacgctttac cacggcattt attttagtga attactcttt catgccgcgc gaatggcgga
    22801 agagaaaggg cggcagttgc tattggcaga tggtaaacac agcgccgaag aagagcgcca
    22861 ggcgattcag tatctgctgg atttgcgctg cgacgccatt atgatttacc cgcgtttttt
    22921 aagcgtggat gagatcgatg acatcattga cgcgcacagt cagccgataa tggtgcttaa
    22981 tcgccgcctg cgcaaaaaca gcagccatag cgtctggtgc gatcataaac agaccagctt
    23041 taatgccgtg gcagagttga taaacgccgg acatcaggag attgctttcc ttaccggctc
    23101 gatggattcc cccaccagca ttgaacgtct tgctgggtat aaagacgcgc tggcgcagca
    23161 tggtattgcg ctcaatgaaa aacttatcgc taacggtaaa tggacgcccg ccagcggggc
    23221 cgaaggggta gaaacgttgc tcgaacgtgg ggctaaattt agcgcgttag ttgccagtaa
    23281 cgacgatatg gcgataggtg cgatgaaagc gttgcacgag cgcggcgtag cggtgccaga
    23341 gcaggtgtca gttatcggat tcgatgatat cgcaattgcc ccctatatcg ttccggcgct
    23401 ctccagcgta aaaattccgg tgactgagat gattcaggaa attattggac ggctgatttt
    23461 tatgctcgat ggcggggatt tctcaccgcc gaaaaccttc agcggaaaac tgatccgccg
    23521 cgactcactc attgctcttt cgcgataaac attgtcatca catgtcatcg tcactagtgt
    23581 cgatgacatc ttttttcctg cattacagaa attaatttcc ttaaataaca gtgaattaaa
    23641 actggcatga tttgtgaatg tatcggcgca ttaactgtca ttgctggaga atttgatgaa
    23701 ccgtttcatc attgctgacg cgagtaaatg tattggttgc tgtacctgtg aagtggcgtg
    23761 cgtggtttct catcaggaaa atcaggactg tgcatcgctg accccggaaa catttttacc
    23821 gcgtatccat gtcattaaag gtgtgaacat ttccacggcg acagtttgcc gtcagtgtga
    23881 agatgcaccg tgcgctaacg tctgcccgaa tggtgctatc agccgtgata aagggtttgt
    23941 tcatgtgatg caggaacgtt gcattggttg caaaacctgc gttgtggctt gcccgtatgg
    24001 cgcgatggaa gtggtggtac gtccggtgat tcgcaacagc ggcgcggggc tgaatgtacg
    24061 ggctgacaaa gccgaagcca ataaatgtga cctgtgcaac catcgtgaag acggcccggc
    24121 gtgtatggcg gcttgcccga ctcatgcgct gatttgtgtc gatcgtaata aacttgaaca
    24181 actgagtgca gaaaaacgcc gccgtacggc gctgatgttc taaaaatctt acagaactgt
    24241 tctggcatca aaggaattcc cggaaatatt cgtagaatac ggagctggcg gtatcgccga
    24301 cctgagactg ccggatacga cgcagtcgtg tccggcagga cgggttacgc aattcatgat
    24361 ggcgggaata actcaatggc aaaaaacaca tcttgcggtg tccaactgcg tattcgtggc
    24421 aaagtgcagg gcgtcggttt tcgtccgttt gtctggcagc tggcacagca attaaatctt
    24481 cacggcgatg tctgtaatga cggcgatggc gtagaagtcc ggctgcggga agacccggaa
    24541 acgttacttg ttcaattgca tcagcactgc ccgccgctgg cgcgtattga tagcgtcgag
    24601 cgtgagccgt acatctggtc acaactgccc actgagttca ccatccgcca gagcgcggga
    24661 ggtgccatga atacgcaaat tgtcccggat gccgctactt gccctgcttg ccttgccgaa
    24721 atgaataccc caggcgaacg gcgttatcgt tatccgttta tcaactgtac ccactgcggc
    24781 ccgcgtttca ccattattcg cgccatgcct tacgaccgcc cgtttaccgt gatggcggcg
    24841 tttccgttgt gtccggcttg tgacaaagag taccgtgacc cgctcgatcg tcgcttccac
    24901 gcccagccgg tggcctgccc ggagtgtggc ccacatcttg aatgggtaag tcatggtgaa
    24961 catgcagaac aagaggcggc attacaggcg gctatcgcac agttaaaaat gggcaacatt
    25021 gtcgccatca aagggattgg cggatttcat cttgcctgcg atgcacgtaa cagtaacgcg
    25081 gtggcgacac ttcgggcgcg caaacatcgc ccggcgaaac cgctggcggt catgttgcca
    25141 gtggctgacg gtttaccaga cgctgcgcgc cagttgctta ccacgcccgc cgcgccgatt
    25201 gtgctggtgg ataaaaaata cgttcctgag ctttgtgatg atatcgcccc tgaccttaac
    25261 gaagtcgggg taatgttgcc tgcgaacccg ctccagcatt tgctgttaca ggaactgcaa
    25321 tgcccgctgg tgatgacctc cggcaacctg agcggtaaac caccagctat cagcaacgaa
    25381 caggcgctgg cggatttgca gggcattgcc gacggattct tgatacataa ccgcgacatc
    25441 gtgcagcgga tggatgattc ggtggtgcgc gaaagcggcg aaatgctgcg ccgttcgcgg
    25501 gggtatgtgc cggatgcgct ggctttgcct ccgggcttta aaaatgttcc gcctgtgctg
    25561 tgtctcggcg cggatctgaa aaataccttc tgcctggtgc gcggtgaaca agcggtgttg
    25621 agtcagcatc tgggcgattt aagtgacgat ggcatccaga tgcagtggcg cgaagcgtta
    25681 cgcctgatgc aaaacatcta cgattttacc ccgcaatacg ttgtgcatga cgcgcatccg
    25741 ggctatgtct ccagccagtg ggcgcgtgaa atgaatctgc cgacgcaaac ggtactgcat
    25801 catcatgccc atgcagcggc gtgtctggca gagcatcagt ggccgttgga tggcggtgat
    25861 gtcattgctt tgacgctcga cggtatcggt atgggggaga acggcgcttt gtggggcggc
    25921 gagtgcctgc gggtgaacta tcgcgaatgt gagcacctgg gcggcttgcc tgcagtggcg
    25981 cttccgggtg gcgatttggc agcgaagcag ccgtggcgaa acctgctggc gcagtgcctg
    26041 cgctttgtgc cggagtggca gaattaccct gaaacagcaa gtgtgcaaca gcaaaactgg
    26101 agcgtgctgg cgcgggccat tgagcgtgga attaacgcgc cgctggcgtc atcgtgtggg
    26161 cggttgttcg atgcagtggc ggcggcactg ggctgtgcgc cagccacgtt aagttatgaa
    26221 ggtgaagcgg cttgtgctct ggaggcgctc gcagcctcat gccacggagt gacgcatccg
    26281 gtgacgatgc cgctggtgga caatcaactg gatctcgccg ctttctggca gcagtggctg
    26341 aactggcagg caccggttaa tcaacgcgcg tgggcgtttc atgatgcgct ggcgcagggt
    26401 tttgccgcgt tgatgcgtga gcaggccacg atgcgtggta tcactacgct ggtatttagc
    26461 ggcggggtta ttcataaccg tttgctgcgt gcacgtctgg cgcattatct cgctgatttc
    26521 acattgctct ttccacagag tttaccggcg ggtgatggcg gtttgtctct ggggcagggg
    26581 gttattgctg cggcgcgttg gttagcgggt gaagtccaga acggataaaa cgtgttgcat
    26641 ttggacacca ttgccggatg cggcgtaaac gccttatctg gcctacacat gcgtccccgt
    26701 aggtcggata agacgcgcca gcgtcgcatc cgacatttta ggcacagtag cccacctaca
    26761 tcggcaatgt tttcaacaat ccaaatgcct ctttcatccg atcctcactg accacaaagg
    26821 cgcgaagctg gtcagcatcg tcaacaccgc gagccaccat tccctggcgt tcggtattaa
    26881 tttgccagcg taaatcctga cgctgggttt cgcctgccag atgcagcggt aattccggcg
    26941 ttttgatttt caccagcatc gccggtagtt tcagcggcgt gttattgccg agaagatttt
    27001 ttgccagcac catcgcgcta agttgaatcg gctgcaggaa tggcagtacc tgaccgttga
    27061 tttccgcgca atcgcccagc gcataaatat cgtcattact ggtttgcaga taactatcga
    27121 cgcaaacgcc gcgattaatc gtcagcccgg cgcgtcgtgc cagggctgtt tccgggcgca
    27181 gtccggtggc ggcaattacc gcatccactt cgatactacg ctggtggtcc agcgttgcca
    27241 gaatgccaga atccgttttt tccagcccct gcaactgaga tttcaacagc agatgaacgc
    27301 ccatctccgt caaccgatgc tgcaagcggc tgcttacttc tggtggcatt aatgacgcca
    27361 gaatactggc agcgttgtcg attagcgtga ccgctttgcc tgcacgacaa aaatccatcg
    27421 ccagttcgct accaatcaaa ccaccgccga caatcaacac gcgtcgggca tcccgcagtt
    27481 gcgtttcaca ggcgcgatac tcttgctgac tatttaacgt cagcattaac tcacgcccag
    27541 gcactggcgg gacaaaggca ctggcaccag ttgccagcac cagcttgtcg tattgccact
    27601 gattattctg gcttttcacc acatgggctt cggcatcgat atccgtcacc caggtgtgcg
    27661 gaaacaggtg cagattaaac tgcccggcaa attcacccgc cgtctggcgg gtaaggtcgt
    27721 cggcacgttg cccctggctg ataacatggc tgaggtccgg tttgttgtac tcatccatgc
    27781 tgtcggcggc gatcagggtt aatggaatac tggcgtcctg tttgcgaata tttttcacca
    27841 gttggcgggc ggcgaagccc gaaccaatga tcacaatgcc gttgctcatt ttgcctccga
    27901 tgccagttcg tcaaagacgt ctttgccgag agagcattcc gggcagagga agttatccgg
    27961 aacttcactc cacggcgttc ctggcgcaac gtcctgcatt ggctcgcctt ttgccggatc
    28021 gtaaatccac tggcagacgc tgcactgcat ccgtgggccg aggtcggact tcgtggtggc
    28081 agaggtttct tctttaacca ccgtattcac cgtgctctgc ggcagcggcg cgagcgccca
    28141 ctgacgggcg atttcgcgac catgttcacg gcataactcc agagcgtcct ggtctggtcg
    28201 ccatttggct ttcaggctaa gcgacatttc gaaacccgca tcctgcaggc gcgtggaaag
    28261 acgatccacc gcaccgccgc tccagccgtg agagccgaaa gcactggcgc gtttgttacg
    28321 gaagcgtaat ccggtcatct cctccaccag accggcgatt ttcggcatca tcacgttatt
    28381 catcgtcgaa gtgccgacca gcacgccttt tgagcggaag acattggtca ggatttcgtt
    28441 tttatcgctt cgggcgacgt tgaaaatttt caccgccacg cgtgggtcgg tttccgcaat
    28501 cccctgggcg atagcgtcag ccatcatgcg ggtgttattc gacatggtgt cgtagaaaat
    28561 ggtgattctg tcttcctgat aatcagccgc ccatttcagg tacagctcga caatttgcgt
    28621 cgggttatcg cgccatacca cgccgtgaga ggtcgctatc atatcgactg gtaagttaaa
    28681 gcccaggatc tcggtaattt tcggtgttac caggcggctg aacggcgtca ggatattggc
    28741 gtagtaacgc tggcactgct cgaaaagctc cgtctgatcc acttcatcgt tgaacagatg
    28801 ctcgtcgcag tagtgctgac cgaaagcatc gttactgaac agcaccgcgt cgcctgtcag
    28861 gtaagtcatc atgctgtccg gccagtgcag cattggcgtt tcgacaaaaa tgagctgttt
    28921 gccgttgccg atatccagcg tgtcaccagt tttcaccaca ttaaaattcc actccggatg
    28981 atgatgatga ccatttatcg agtcgatagc gttggcagta cagtaaatcg gcgtatcggg
    29041 aatttgcgcc atcagttcgg tcagcgcccc agcgtggtcc tcttctgcat ggttaatcac
    29101 gatgtaatcg atatccgcca gatcgatttc attacgcagg ttctgcacaa attcgcggct
    29161 gaatttatgg tcgacggtgt cgatcagcac gtttttttct tcgcggatga ggtagctgtt
    29221 gtaactgctg ccgcgtagcg ttttatattc cgtaccgtga aaatcacgca cttcccagtc
    29281 acgttgacca acccaatgaa tgttattttt caccacaata gacatagcaa cctcaattta
    29341 ttcagcgtgt tctaaaaaga tgtcttgcta attgcagatt acgtgccagt tttttatctg
    29401 attgttttta ctctataaat aaaattatgc tcattaatga gtagtcaaaa tgactatcaa
    29461 tcaaatggtc gatatgacaa tatctatagt caaattgaca gtgaggcaaa gatgagtttt
    29521 tccgttgatg tgctggcgaa tatcgccatc gaattgcagc gtgggattgg tcatcaggat
    29581 cgttttcagc gcctgatcac cacgctacgt caggtgctgg agtgcgatgc gtctgcgttg
    29641 ctacgttacg attcgcggca gtttattccg cttgccatcg acggtctggc aaaggatgta
    29701 ctcggtagac gctttgcgct ggaagggcat ccacggctgg aagcgattgc ccgcgccggg
    29761 gatgtggtgc gctttcccgc agacagcgaa ttgcccgatc cctatgacgg tttgattcct
    29821 gggcaggaga gtctgaaggt tcacgcctgc gttggtctgc cattgtttgc cgggcaaaac
    29881 ctgatcggcg cactgacgct cgacgggatg cagcccgatc agttcgatgt tttcagcgac
    29941 gaagagctac ggctgattgc tgcgctggcg gcgggagcgt taagcaatgc gttgctgatt
    30001 gaacaactgg aaagccagaa tatgatgcca ggcgatgcca cgccgtttga agcggtgaaa
    30061 cagacgcaga tgattggctt gtcccctggc atgacgcaac tgaaaaaaga gattgagatt
    30121 gtggcggcgt ccgatctcaa cgtcctgatc agcggtgaga cgggaaccgg taaggagctg
    30181 gtggcgaaag cgattcatga agcctcgcca cgggcggtga atccgctggt ctatctcaac
    30241 tgtgctgcac tgccggaaag tgtggcggaa agtgagttgt tcgggcatgt gaaaggagcg
    30301 tttactggcg ctatcagtaa ccgcagcggg aagtttgaaa tggcggataa cggcactctg
    30361 tttctggatg agatcggcga gttgtcgttg gcattgcagg ccaagctgct gagggtgttg
    30421 cagtatggcg atattcagcg cgttggcgat gaccgtagtt tgcgggtcga tgtgcgcgtg
    30481 ctggcggcga ctaaccgcga cttacgcgaa gaggtgatgg cagggcgatt tcgcgctgac
    30541 ttgtttcatc gcctgagcgt gtttccactt tcggtgccgc cgctgcgtga gcggggcgat
    30601 gatgtcattc tgctggcggg gtatttctgc gagcagtgtc gtttgcggct ggggctctcc
    30661 cgcgtggtat taagtgccgg agcgcgaaat ttactgcaac actatcgttt tccggggaac
    30721 gtgcgcgaac tggaacatgc tattcatcgg gcggtagtgc tggcgagagc cacccgcaac
    30781 ggcgatgaag tgattcttga ggcgcaacat tttgcttttc ctgaggtgac gttgccgccg
    30841 ccagaagcgg cggcggtgcc cgttgttaag caaaacctgc gtgaagcgac agaagcgttc
    30901 cagcgtgaaa ctattcgcca ggcactggca caaaatcatc ataactgggc tgcctgcgcg
    30961 cggatgctgg aaaccgacgt cgccaacctg catcggctgg cgaaacgtct gggaatgaag
    31021 gattaaataa tcccggcctg atagaaatcc tgcaggttaa ttgcgccggt gagtttgccg
    31081 ttttcatcca ccaccggtgc ggcagtgatt ttgcgcttca tcaggatctc tttggcgtcg
    31141 atggcgcgac tttgcgcctg taacgtggtg ccgcctgtcg tcatcgcttc attaactggc
    31201 gtggtgagtg cgccgccgcc taccagccag cgacgtaaat cgccgtcggt aaagacgcct
    31261 tgtacctgtt gttgagcgtc acataccgcc accagcccca gaccggtgcg gctgagttcc
    31321 agcatcgcat ccatcacgct ggcggttaac gccacctgtg ggatggcatc gtcacggcgc
    31381 atcagatgat gcactttatt cagcaagcga gcgcccagtg ccccggctgg gtgggagcgg
    31441 gcaaaatctt cttcattaaa tccgcgcgcc tgcatgaccg ccatcgccag cgcgtcaccc
    31501 atcatcaggg tattgacggt gctggaggtc ggcgcaaggt gcatcgggca ggcttcgcgt
    31561 tctacggaga tatccagcac cgcttttgcc gccaggccca gcggtgacgt cggtttgccg
    31621 gtcatcgcca gcagcgcgat agatttatct tccagacgcg gaataatcag atccagttcc
    31681 ttcgcgccac cggagtaaga gataaacagc atcacatcgc ggctttcaat catccccaga
    31741 tcgccgtgca gtgcttctgc cggatggaca aaaaaagccg atgtgccggt actggcaagt
    31801 gttgcggcga ttttcttacc aatgtggccc gatttgccaa ttcccgaaac caccactttg
    31861 ccttcgcagt gcaggatgat attggcggcg cgaacaaaat catcgcccag acgttccggt
    31921 aaacggcttg cttcctgcaa ctccagcatt aacgtctgac gtcccgcgtt cagtagtgct
    31981 tcactcattg ctctctccgg ttatgatcac atcgatccct ttctcttcca gcgcctgacg
    32041 aaacgccgga tcgatacctg cgtcggtaat cagcttatcg acgctttcaa gactgcaaac
    32101 tacgttgggg cttttacggc caaactttga tgagtccgcc atcaaaatca cttcgcgcgc
    32161 ggcattgcac attgccttac tgacggtata aacctcgtta aaggtggtta cgcccgcatt
    32221 gagatcgatg ccgtcggtgc ccataaacaa tttatcgaaa ctgaaatgtt cgaaggcatt
    32281 ctctgccagc tgtccgtgaa atgaggccga ttttttacga aacgttccgc ctggcatcag
    32341 gatagtttgt tcgttatcca gttcagatag cgcattgacg atatgcagac tgttggtcat
    32401 caccgtgatg ttattaaagc gcgagagcag gggaaccatc tgcaaaacgg tactgccagc
    32461 atcaagaatg atcgaatcgc catcatgaat aaaactaacg gcagcttctg ctatcagctc
    32521 tttcttgtgg gtgttgatga gtgttttatg atcgataggc ggatcggatt cctctttatt
    32581 caacaccact ccgccataag tacgaatgac ggttcctgca tgttccagaa tgaccagatc
    32641 tttgcgaatg gttgtgcctg tggtgtcaaa gtattgcgcc aattcttcaa ccgagcattt
    32701 accctgcttt tgcagatact ccagaatggc ggcctgacgc tgacgaggtt tcatgggcgt
    32761 gattacctga tttaggtttc aaaatgataa cttcgcaagc aagtaacatt cacaacgaaa
    32821 ttatccacgt ttcagtttaa gcgccaatga tagagcattc tgtgatagtg gatcatgggg
    32881 aaagatcaca tcgggctgta aatcacccgc atgcataccg gtaattgcgg gaattttttg
    32941 cggtcgtgcg aagacggtca gtcctttcat aaacaaggtg tcgacgatgc gctgctgatc
    33001 gtccagggcg atggcgacca cgacccgtgg tttaaagcgc ccgctggaac ggcccacgcc
    33061 aacccgccct tgctggcata gtgtgtcgaa ggcacggtta aaacgagaaa tttgccagcc
    33121 gcctaaggcc agttgcgcac accaggcgat aacggcgacg gtgatgagtg cggataccat
    33181 aacttttcct taatgtagtg gcggaagtcc tgtatgcctg atgagcgtag cgcatcaggc
    33241 attctattag tctcacggag atccttaacg gatctccgct gttgattaca acatcacctg
    33301 accgccggtg acattgatcg actgtccggt gcagtacgac gccttaggac tggcatagag
    33361 cagcagcata ttcagcacat cctgataatc gcagccgcgt ttgagcggta ctttgtcgat
    33421 gtaatactgc tcgacttgat ccggtttgat acccagcttg gtcgcgtatt ggggtaacag
    33481 tgactggaac ataggcgatt tcagcaggtt accgagcatc agtgaatgca ccgtaatgcc
    33541 gtactccgcc agatccagcg ccagtgattg agtcagcccg acgccaccaa atttcgctgc
    33601 gctgtagcca gagttgtgtt tgctgcccac tttgccggat ttcgagttga tctgaataat
    33661 gcgaccctga atcccgtcgc ggatcatcaa acgagaaaat tcacgggcac acaggaaata
    33721 acccaccaga ttcacggtca aaatcgccga gctggaagtc gctgataaag gctgcttttg
    33781 ctattccggc gctgtagacc agcaaatcca cgcgcccaaa gatttcatct accccacgag
    33841 agagcgccag gacgctttgc tcgctggtgg cgtcagcacc aaaactatac gctgttcctt
    33901 caccatattc ggcgttaatt tcttgtgcca catttgcggc tttgtcgctc tgaatatcga
    33961 caaccgcgac gcgatacccc tcggcagcca gaccgtggca caggaacgcg cctaaggttt
    34021 gcccaccacc gatgacaacg gcaacctgat tcatttttta ctccttaaca gattcaaact
    34081 tcaaaaccga tcccggcgcg atatcgtcgg gtacagggcc tgccacatgg acagtgcccg
    34141 gaaattccgc ttcgtttaaa ccatcgaagc gcagggtgac atgacccagt tcgcgaaggt
    34201 tgtcttccgc cacgctgcca acagcggtca ccggatagcg atgctgcccg agcgtaaatt
    34261 gcaaaccggg atggagtgca cctttcaact cgccgtggca atgaatgaag caatactctt
    34321 cgaggtccgc tggcgcgcct tcacgaaagg tgatgagcat ctggtcgctg agggcgtcag
    34381 tggcactcgc gccgatacgg gtgatggtgg tctgataaat aacggtcatg tttcagcctc
    34441 tattgataga taaaaccgga gacaaaccag gcgatcagta cagtcggtgc gccggttaaa
    34501 aagtggctca ccagtacaga agggacaccg acgcgaaccg tgtcctgacg ggcttccgcc
    34561 agcgacaaac cgaccgggat gaagtcgcag gccgcctgcg cgttgatggc aaacagtgcc
    34621 ggtaaagcca gatgcggcgg aatattgccg agaccaatct gcacgccaat caatacgccg
    34681 ataacctgtg cgataactgc gcctgggccg aggaaaggtg aaagcagcgg gaaggagcag
    34741 atgagcgcca gcatgaccag acctagtgga tggctcgcca gcggagcaag accgtgggca
    34801 atccagtcac caaggccaga agccataatg atgccaatga gcgccgagac gaacgccata
    34861 aacggcagaa tggtttttaa tacagtgtcg atggtgtcac gaccagattg aaacaacacg
    34921 gcaacggcag aacccatacc cattcccacc ttcgccagta aaccatcgct ttgttcggtg
    34981 attttcttgc tggtgtcata gtcacggccc acggaagagg gttgtggtgt cgcatcaccc
    35041 actacagtga tgttttcttc ttttacgcca gagacataaa tatcttccac aatgtactgc
    35101 gccagcggac tggacttgcc cgtcgagtgg atattaatgg tgggaatacg tcgtttcgga
    35161 tagatgccgc agcgtaatgt gccgccacag tcgattaccg cgacaccaat ttccgcctcc
    35221 gcgggttcac cttctttaaa tccgtcaata gcctgccagc cagtaagttg tgccagtttg
    35281 tcaacaatcg caggccgggt accggcggtg atatagacga tttttttgcc cggcgtggct
    35341 tccagctcaa gcgggccgcc ccagccaccc gttccttttt cgatccgaat acgcgtcatg
    35401 atgttgctcc tgccaggtga actttttgtt caagttgaat gcccatcttt ttctcaaaaa
    35461 tggcggtggt cagatcggtc acccagccgc gaaagaagtt ggtgaccaga ccaaccagca
    35521 gatagctcac cgccagtggg ccaagtggca ggttcagcgt tgtcagaccg ctggcaatgc
    35581 caagataaac aaacagttcg ccagggttga tatggagaaa gaggccattc attgagtggc
    35641 agctataaga ggctgccgcg tagtagctgg gtttgtactt ttccggcata aagcgaccca
    35701 ggcttaaggt catcggattg caaaagacaa acgtgccaat gcacggtagc agcaggtaac
    35761 gggaaacagg gttaccggcg caacgttgag caaaacgttc aatacgatgc tgaccgataa
    35821 aattaatcag tgcgttcatg ataaccagca ggctaatcaa cagcggaaga atgccggtca
    35881 ccatcccggt aaacacctct ccgccctttt ggaacagccc gataaaccac tctgcaccat
    35941 gagtaatggt ttctatcatt gttctctcct tcaggattta ttgttttatt accaaacggc
    36001 aacctaatct aatcagattg aaagatttga aagtgttatt ttgatcgcaa aatgaaagat
    36061 aaatatttta atttgaaagt ttgaataaaa ggatagcgag gggaatgagt tgagttatgt
    36121 aaagtccgta tcgggcagtg actaccgctt ccttgtgcgg ggcgtgatgc tttaccatac
    36181 ttgcccctgg ttgaatctgt taaatggacc cctcatgttc aagcgtcgtt atgtaacatt
    36241 gcttcccctt tttgtgttgc ttgccgcctg tagcagcaag ccaaaaccta ctgagactga
    36301 aacgaccacc ggaacgccgt ctggcggctt cctgcttgag cctcagcaca atgtgatgca
    36361 gatgggcggc gatttcgcta ataacccgaa tgcccagcag ttcatcgaca gaatggtgaa
    36421 caaacacggt ttcgatcgtc agcagttgca ggaaattctc tcccaggcga agcgtctgga
    36481 ttcggtactg cggctgatgg ataaccaggc accaaccaca tcggtgaaac cgccatcagg
    36541 tccgaacggc gcatggctgc gttatcgcaa aaaatttatt acgccggaca acgtgcagaa
    36601 cggtgtggtt ttctggaatc agtatgaaga tgcgttgaat cgcgcgtggc aggtgtatgg
    36661 cgtaccgccg gaaattatcg tcgggattat cggcgttgaa acccgctggg ggcgcgtgat
    36721 ggggaaaact cgcatcctcg atgcgctggc aacgctgtca tttaactacc cacgccgcgc
    36781 ggagtatttc tctggcgagc tggaaacctt cctgttgatg gcgcgcgacg agcaggacga
    36841 tccgctcaat ctgaaaggtt cctttgccgg ggcgatgggc tacggacagt ttatgccgtc
    36901 gtcttacaaa caatatgcgg tagatttcag cggcgacggg catatcaacc tgtgggatcc
    36961 ggttgatgcg atcggtagtg tggcgaacta tttcaaagcg cacggctggg tgaaaggcga
    37021 tcaggtcgcg gtaatggcaa acggtcaggc tccaggcttg ccaaatggct tcaaaaccag
    37081 gtacagcatt tcgcagcttg ccacggcagg tttaacgcca cagcagccgc tgggcaacca
    37141 tcaacaagcc agcctgctgc gtctggatgt cggtaccggt taccagtact ggtacggtct
    37201 gccgaacttc tacaccatta cccgttataa ccacagcacc cattacgcaa tggcggtctg
    37261 gcagttagga caagccgtgg cgctggcgcg cgtgcagtaa ctatttgcaa gaggggggcg
    37321 tgagcttccc tctggctaat cagctttttt ctgaatcctc ctcgtaaaat tgcaacgcca
    37381 acaccatctt cctgacgaaa gtgctatctt gtccggcata aatttttgac tgactgaggt
    37441 tgtgatgact gacagtgaac tgatgcagtt aagcgaacag gttgggcagg cgctgaaagc
    37501 ccgtggcgca accgtaacaa ctgccgagtc ttgtaccggt ggttgggtag cgaaagtgat
    37561 taccgatatt gccggtagct ccgcctggtt tgaacgcgga tttgtcacct acagtaacga
    37621 agccaaagcg cagatgatcg gcgtacgcga agagacgctg gcgcagcatg gcgcggtgag
    37681 tgaacccgtc gtggtggaaa tggcgatagg tgcactgaaa gcggctcgtg ctgattatgc
    37741 cgtgtctatt agtggtatcg ccgggccgga tggcggcagt gaagagaagc ctgtcggcac
    37801 cgtctggttt gcttttgcca ctgcccgcgg tgaaggcatt acccggcggg aatgcttcag
    37861 cggcgaccgt gatgcggtgc gtcgtcaggc tactgcgtat gcattgcaga ccttgtggca
    37921 acaatttcta caaaacactt gatactgtat gagtatacag tataattgct tcaacagaac
    37981 atattgacta tccggtatta cccggcatga caggagtaaa aatggctatc gacgaaaaca
    38041 aacagaaagc gttggcggca gcactgggcc agattgagaa acaatttggt aaaggctcca
    38101 tcatgcgcct gggtgaagac cgttccatgg atgtggaaac catctctacc ggttcgcttt
    38161 cactggatat cgcgcttggg gcaggtggtc tgccgatggg ccgtatcgtc gaaatctacg
    38221 gaccggaatc ttccggtaaa accacgctga cgctgcaggt gatcgccgca gcgcagcgtg
    38281 aaggtaaaac ctgtgcgttt atcgatgctg aacacgcgct ggacccaatc tacgcacgta
    38341 aactgggcgt cgatatcgat aacctgctgt gctcccagcc ggacaccggc gagcaggcac
    38401 tggaaatctg tgacgccctg gcgcgttctg gcgcagtaga cgttatcgtc gttgactccg
    38461 tggcggcact gacgccgaaa gcggaaatcg aaggcgaaat cggcgactct cacatgggcc
    38521 ttgcggcacg tatgatgagc caggcgatgc gtaagctggc gggtaacctg aagcagtcca
    38581 acacgctgct gatcttcatc aaccagatcc gtatgaaaat tggtgtgatg ttcggtaacc
    38641 cggaaaccac taccggtggt aacgcgctga aattctacgc ctctgttcgt ctcgacatcc
    38701 gtcgtatcgg cgcggtgaaa gagggcgaaa acgtggtggg tagcgaaacc cgcgtgaaag
    38761 tggtgaagaa caaaatcgct gcaccgttta aacaggctga atttcagatc ctctacggcg
    38821 aaggtatcaa cttctacggc gaactggttg acctgggcgt aaaagagaag ctgatcgaga
    38881 aagcaggcgc gtggtacagc tacaaaggtg agaagatcgg tcagggtaaa gcgaatgcaa
    38941 ctgcctggct gaaagataac ccggaaaccg cgaaagagat cgagaagaaa gtacgtgagt
    39001 tgctgctgag caacccgaac tcaacgccgg atttctctgt agatgacagc gaaggcgtag
    39061 cagaaactaa cgaagatttt taatcgttcg ttattgatga taatagataa tgtgtgcacg
    39121 gacggtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc caacactggc
    39181 gtgaaaaatt actctcacct cgatcctcta acgaattggc tgcacactca ttgactatta
    39241 aatctgttta tcagcaaaac ctgggtcgca tctgcggccc ttttgctttt ttaagttgta
    39301 aggatatgcc atgacagaat caacatcccg tcgcccggca tatgctcgcc tgttggatcg
    39361 tgcggtacgc attctggcgg tgcgcgatca cagtgagcaa gaactgcgac gtaaactcgc
    39421 ggcaccgatt atgggcaaaa atggcccaga agagattgat gctacggcag aagattacga
    39481 gcgcgttatt gcctggtgcc atgaacatgg ctatctcgat gacagccgat ttgttgcgcg
    39541 ctttatcgcc agccgtagcc gcaaaggtta tggacctgca cgtattcgtc aggaactgaa
    39601 tcagaaaggt atttcccgcg aagcgacaga aaaagcgatg cgtgaatgtg acatcgactg
    39661 gtgcgcactg gtgcgcgatc aggcgacgcg aaaatatggt gaacctttgc caactgtctt
    39721 ttcagaaaaa gttaagatcc agcgttttct gctctatcgt ggctatctga tggaagatat
    39781 ccaggatatt tggctaaatt ttgccgactg aacgcatacg gtattttact tcccactcaa
    39841 gaaaacttat cttattccca cttttcagtt accagcccgg cggttaagac acgctggagc
    39901 tggtggcgat atttcgttag cttgatttca ggataattat gagcaagagc accgctgaga
    39961 tccgtcaggc gtttctcgac tttttccata gtaagggaca tcaggtagtt gccagcagct
    40021 ccctggtacc ccataacgac ccaactttgt tgtttaccaa cgccgggatg aaccagttca
    40081 aggatgtgtt ccttggactc gacaagcgta attattcccg cgctaccact tcccaacgct
    40141 gcgtgcgtgc gggtggtaaa cacaacgacc tggaaaatgt cggttacacc gcgcgtcacc
    40201 ataccttctt cgaaatgctg ggcaacttca gcttcggcga ctatttcaaa cacgatgcca
    40261 ttcagtttgc atgggaactg ctgaccagcg aaaaatggtt tgccctgccg aaagagcgtc
    40321 tgtgggttac cgtctatgaa agcgacgacg aagcctacga aatctgggaa aaagaagtag
    40381 ggatcccgcg cgaacgtatt attcgcatcg gcgataacaa aggtgcgcca tacgcatctg
    40441 acaacttctg gcagatgggt gacactggtc cgtgcggccc gtgcaccgaa atcttctacg
    40501 atcacggcga ccacatttgg ggtggccctc cgggaagtcc ggaagaagac ggcgaccgct
    40561 acattgagat ctggaacatc gtcttcatgc agttcaaccg ccaggcagat ggcacgatgg
    40621 aaccgctgcc gaagccgtct gtagataccg gtatgggtct ggagcgtatt gctgcggtgc
    40681 tgcaacacgt taactctaac tatgacatcg acctgttccg cacgttgatc caggcggtag
    40741 cgaaagtcac tggcgcgacc gatctgagca ataaatcgct gcgcgtaatc gctgaccaca
    40801 ttcgttcttg tgcgttcctg atcgcggatg gcgtaatgcc gtccaatgaa aaccgtggtt
    40861 atgtactgcg tcgtatcatt cgtcgcgcag tgcgtcacgg caatatgctc ggcgcgaaag
    40921 aaaccttctt ctacaaactg gttggtccgc tgatcgacgt tatgggctct gcgggtgaag
    40981 acctgaaacg ccagcaggcg caggttgagc aggtgctgaa gactgaagaa gagcagtttg
    41041 cccgtactct ggagcgcggt ctggcgttgc tggatgaaga gctggcaaaa ctttctggtg
    41101 atacgctgga tggtgaaacg gctttccgtc tgtacgacac ctatggcttc ccggttgacc
    41161 tgacggctga tgtttgtcgt gagcgcaaca tcaaagttga cgaagctggt tttgaagcag
    41221 caatggaaga gcagcgtcgt cgcgcgcgcg aagccagcgg ctttggtgcc gattacaacg
    41281 caatgatccg tgttgatagt gcatctgaat ttaaaggcta tgaccatctg gaactgaacg
    41341 gcaaagtgac cgcgctgttt gttgatggta aagcggttga tgccatcaat gcaggccagg
    41401 aagctgtggt cgtgctggat caaacgccat tctatgcgga atccggcggt caggttggtg
    41461 ataaaggcga actgaaaggc gctaacttct ccttcgcggt ggaagatacg cagaaatacg
    41521 gccaggcgat tggtcacatc ggtaaacttg ctgcgggttc tctgaaagtg ggcgacgctg
    41581 tgcaggctga tgttgatgag gctcgtcgcg cccgtattcg tctgaatcac tccgcaacgc
    41641 acctgatgca cgctgcgctg cgccaggttc tggggactca tgtatcgcag aaaggttcac
    41701 tggttaacga caaggtgctg cgcttcgact tctcacacaa cgaagcgatg aaaccggaag
    41761 agattcgtgc ggtcgaagac ctggtgaacg cacagattcg tcgcaatttg ccgatcgaaa
    41821 ccaacatcat ggatctcgaa gcggcgaaag cgaaaggtgc gatggcgctg ttcggcgaga
    41881 agtatgatga gcgtgtacgc gtgctgagca tgggcgattt ctccaccgag ctgtgtggcg
    41941 gtactcacgc cagccgcact ggtgatattg gtctgttccg catcatctct gaatcgggta
    42001 ctgctgcagg cgttcgtcgt atcgaagcgg taaccggaga aggcgctatc accaccgttc
    42061 atgcagacag cgatcgctta agcgaagtcg cgcatctgct gaaaggcgat agcaataatc
    42121 tggcggataa agtgcgctca gtactggaac gtacgcgtca gttagaaaaa gaactacaac
    42181 agcttaaaga acaagctgcc gcacaggaga gcgcaaatct ttccagtaag gcaattgatg
    42241 ttaatggtgt taagctgttg gttagcgagc ttagcggtgt tgagccgaaa atgttgcgta
    42301 ccatggttga cgatttaaaa aatcagctgg ggtcgacaat tatcgtgctg gcaacggtag
    42361 ccgaaggtaa ggtttctctg attgcaggcg tatctaagga cgtcacagat cgtgtgaaag
    42421 caggggaact gattggtatg gtcgctcagc aggtgggcgg caagggtggt ggacgtcctg
    42481 acatggcgca agccggtggt acggatgctg cggccttacc tgcagcgtta gccagtgtga
    42541 aaggctgggt cagcgcgaaa ttgcaataat ataagcgtca ggcaatgccg tggactcgct
    42601 tcacggcatt cgcattaacg ctatcgacaa cgataaagtc aggttgaagt tgtgtatatc
    42661 ggctaaactt aggtttaaca gaatgtaatg ccatgactgc ttacatgtaa tgtgtttgtc
    42721 attgcttact ttttggcgtt atatgatgga taatgccggg atacagagag acccgactct
    42781 tttaatcttt caaggagcaa agaatgctga ttctgactcg tcgagttggt gagaccctca
    42841 tgattgggga tgaggtcacc gtgacagttt taggggtaaa gggcaaccag gtacgtattg
    42901 gcgtaaatgc cccgaaggaa gtttctgttc accgtgaaga gatctaccag cgtatccagg
    42961 ctgaaaaatc ccagcagtcc agttactaat ctttccgcgt ctcatcttta tcggtgagac
    43021 gcaccctcaa aatttctcct tcactctata gtcttttcgc tttactcccg ttcattcaac
    43081 ttaagtctcc atttttttgc attactgcta tctgccagac ctccgttttt ctgtagataa
    43141 aacactcttt ttgacgtttt tacagactaa ttgagcgtga agtgtgcaaa cgataaaagt
    43201 gtgggaaaaa ttgtttgact tataagtctc agaaagtaat atgtgcgcca cgcagcgacg
    43261 atgagcgata aacaagttct tcgaagcact cgtaagaggc gtgtggtgag gtggccgaga
    43321 ggctgaaggc gctcccctgc taagggagta tgcggtcaaa agctgcatcc ggggttcgaa
    43381 tccccgcctc accgccattt gcatccgtag ctcagctgga tagagtactc ggctacgaac
    43441 cgagcggtcg gaggttcgaa tcctcccgga tgcaccatct cttacttgat atggctttag
    43501 tagcggtatc aatatcagca gtaaaataaa ttttcccgat gcatccgtag ctcagctgga
    43561 tagagtactc ggctacgaac cgagcggtcg gaggttcgaa tcctcccgga tgcaccatat
    43621 tctccgaaat cttcagcaat gaaggtatcg aagaggtagc ggaattaacc gcaggcacca
    43681 gggatgataa cgttgcttta gcaacggccc gaagggcgag ccgcaaggcg agtaatcctc
    43741 ccggatgcac catctcttaa ttgatatggc tttagtagcg gtatcaatat cagcagtaga
    43801 ataagttttc ccgatgcatc cgtagctcag ctggatagag tactcggcta cgaaccgagc
    43861 ggtcggaggt tcgaatcctc ccggatgcac catattctcc gtaaccttca gcaatgaagg
    43921 tatcgaagag atagcggtat taaccgcagg caccaggaag gataacgttg cgccagcaac
    43981 ggcccgaagg gcgaacgtca gtgagtcatc ctcccggatg caccatcttc tccaaaaaat
    44041 ttccttcaaa aatatctcat ttctaaataa ctcctttatc catttcctga attttcttct
    44101 ccttcgccat cagcgacaaa atcaagcaat tacgctaaag tagcgtcata tttttcgact
    44161 tgcgagaaga ccatgtacga gcgttatgca ggtttaattt ttgatatgga tggcacaatc
    44221 ctggatacgg agcctacgca ccgtaaagcg tggcgcgaag tattagggca ctacggtctt
    44281 cagtacgata ttcaggcgat gattgcgctt aatggatcgc ccacctggcg tattgctcag
    44341 gcaattattg agctgaatca ggccgatctc gacccgcatg cgttagcgcg tgaaaaaaca
    44401 gaagcagtaa gaagtatgct gctggatagc gtcgaaccgc ttcctcttgt tgatgtggtg
    44461 aaaagttggc atggtcgtcg cccaatggct gtaggaacgg ggagtgaaag cgccatcgct
    44521 gaggcattgc tggcgcacct gggattacgc cattattttg acgccgtcgt cgctgccgat
    44581 cacgtcaaac accataaacc cgcgccagac acatttttgt tgtgcgcgca gcgtatgggc
    44641 gtgcaaccga cgcagtgtgt ggtctttgaa gatgccgatt tcggtattca ggcggcccgt
    44701 gccgcaggta tggacgctgt ggacgttcgc ttgctgtgag tgaagcgtta tcgcttttct
    44761 cgttgtttgc cagtagtttt ctcagcgcta cactattacc cggcaactcc gaagtcgtgc
    44821 tggtggcaat gttgctttcc gggatcagtc atccctgggt tttagtttta acagcaacaa
    44881 tgggtaatag ccttggaggg ttaactaacg ttatccttgg gcgtttcttt ccattgcgta
    44941 aaacatcgcg ctggcaagag aaagctatcg gctggctgaa acgctatggt gcagtcacgc
    45001 tattattaag ctggatgccc gtggttggcg atttactgtg tctgttagcg ggatggatgc
    45061 gcatctcgtg gggaccggta atcttttttt tgtgccttgg taaagcgtta cgctatgttg
    45121 cagttgcagc agcgaccgtt cagggcatga tgtggtggca ctaattgtag gcctgcacat
    45181 atggtcacca ttacagttat gctaattaaa acgattttga caggcgggag gtcaatttga
    45241 tcccggacgt atcacaggcg ctggcctggc tggaaaaaca tcctcaggcg ttaaagggga
    45301 tacagcgtgg gctggagcgc gaaactttgc gtgttaatgc tgatggcaca ctggcaacaa
    45361 caggtcatcc tgaagcatta ggttccgcac tgacgcacaa atggattact accgattttg
    45421 cggaagcatt gctggaattc attacaccag tggatggtga tattgaacat atgctgacct
    45481 ttatgcgcga tctgcatcgt tatacggcgc gcaatatggg cgatgagcgg atgtggccgt
    45541 taagtatgcc atgctacatc gcagaaggtc aggacatcga actggcacag tacggcactt
    45601 ctaacaccgg acgctttaaa acgctgtatc gtgaagggct gaaaaatcgc tacggcgcgc
    45661 tgatgcaaac catttccggc gtgcactaca atttctcttt gccaatggca ttctggcaag
    45721 cgaagtgcgg tgatatctcg ggcgctgatg ccaaagagaa aatttctgcg ggctatttcc
    45781 gcgttatccg caattactat cgtttcggtt gggtcattcc ttatctgttt ggtgcatctc
    45841 cggcgatttg ttcttctttc ctgcaaggaa aaccaacgtc gctgccgttt gagaaaaccg
    45901 agtgcggtat gtattacctg ccgtatgcga cctctcttcg tttgagcgat ctcggctata
    45961 ccaataaatc gcaaagcaat cttggtatta ccttcaacga tctttacgag tacgtagcgg
    46021 gccttaaaca ggcaatcaaa acgccatcgg aagagtacgc gaagattggt attgagaaag
    46081 acggtaagag gctgcaaatc aatagcaacg tgctgcagat tgaaaacgaa ctgtatgcgc
    46141 cgattcgtcc aaaacgcgtt acccgcagcg gcgagtcacc ttctgatgcg ctgttacgtg
    46201 gcggcattga atatattgaa gtgcgttcgc tggacatcaa cccgttctcg ccgattggtg
    46261 tagatgaaca gcaggtgcga ttcctcgacc tgtttatggt ctggtgtgcg ctggctgatg
    46321 caccggaaat gagcagtagc gaacttgcct gtacgcgcgt taactggaac cgggtgatcc
    46381 ttgaaggtcg caaaccgggc ctgacgctgg gtatcggctg cgaaaccgcg cagttcccgt
    46441 tactgcaggt gggtaaagat ctgttccgcg atctgaaacg cgtcgcgcaa acgctggata
    46501 gcattaacgg cggcgacgcg tatcagaaag tgtgtgatga actggttgcc tgcttcgata
    46561 atcccgatct gactttctct gcccgtatct taaggtctat gattgatact ggtattggcg
    46621 gaacaggcaa agcgtttgca gaagcctacc gtaatctgct gcgtgaagag ccgctggaaa
    46681 ttctgcgcga agaggatttt gtagccgagc gcgaggcatc tgaacgccgt cagcaggaaa
    46741 tggaaaccgc tgataccgaa ccgtttgcgg tgtggctgga aaaacacacc tgacagaaaa
    46801 gaaaaaggcc actcgtgagt ggccaaaatt tcatctctga attcagggat gatgataaca
    46861 aatgcgcgtc tttcatatac tcagactcgc ctgggaagaa agagttcaga aaatttttaa
    46921 aaaaattacc ggaggtggct aaatgccgtt gttagatagc ttcacagtcg atcatacccg
    46981 gatggaagcg cctgcagttc gggtggcgaa aacaatgaac accccgcatg gcgacgcaat
    47041 caccgtgttc gatctgcgct tctgcgtgcc gaacaaagaa gtgatgccag aaagagggat
    47101 ccataccctg gagcacctgt ttgctggttt tatgcgtaac catcttaacg gtaatggtgt
    47161 agagattatc gatatctcgc caatgggctg ccgcaccggt ttttatatga gtctgattgg
    47221 tacgccagat gagcagcgtg ttgctgatgc ctggaaagcg gcaatggaag acgtgctgaa
    47281 agtgcaggat cagaatcaga tcccggaact gaacgtctac cagtgtggca cttaccagat
    47341 gcactcgttg caggaagcgc aggatattgc gcgtagcatt ctggaacgtg acgtacgcat
    47401 caacagcaac gaagaactgg cactgccgaa agagaagttg caggaactgc acatctagtc
    47461 agtaaactat cttcacaatt aattgaaaaa agccagttca aatgaactgg cttagttgta
    47521 cttagtgcgc accgccgcca ccgccacctg cgccaaatgg cggtttagca aaccacacca
    47581 gccccagcag tacgaggaat atcccggctg acatccagaa gatctcattg gcggaaataa
    47641 tcagcccctg attggtgatc tgctgggcaa tccagcctga tgcctgctgt tgcgtcatcc
    47701 caagcccttc cagttgactg tacatcgcct gggcattcgg gttgaacggg tttaccgact
    47761 cagtcaactg cgcatggtgc atcgactcgc ggttggtcca catggtcgtg gttatcgacg
    47821 tgccgataga ccccgccagc gttcgcgtaa agttagagag gctcgatgcc gccgccagtc
    47881 gttccggtgg caaaccagac agcgtaatgg tggtcagcgg cataaagaag caggccaccg
    47941 caaacccctg gataaactgc ggccaggccg acgcgccaaa atccatacct ggttcaaagg
    48001 tataggcacg ccagtagaag cagacggcat acataataaa gctgaaggtt accagccgcc
    48061 gcatatccag tttatgcgcg aagcggccga taatcggcga caggatcacc ggaataatcc
    48121 ctaccggcgc agaggccaaa cctgcccagg tcgccgtgta accgtagacc tcctgcaaca
    48181 actgcggcag cagaacaata gcgccgaagt agagcatata cgcgaggctg atacacaagc
    48241 agccgatggt gaagttgcgc gacttaaaca acgacagatc gactatcggg ttatcgtcgg
    48301 tcagctccca gacaatcagg aagcagatag ccaccaccgc caccacggta aggatgataa
    48361 tttcctgtga tgaaaaccag tccagctctt taccgcggtc gagcataatc tgcaggctgc
    48421 cgataccaat aaccagcagt gccagcccca cggcatcaat ccgccgccgt ttggtgcggg
    48481 tttcacgtcc gcgcagagtt tgcagtgtca tcaacaccac cgccacgcca atcggcacgt
    48541 tgatgaagaa tatccagccc cagtggtaat tatcgctgat ataaccgccg aggatcgggc
    48601 cgcaaattgg cgcgacaatc accgtcatcg accacaacgc cagcgcgatc gagcgtttgg
    48661 ctggcgggta gttattcagc aatagacttt gcgaaagcgg gatcaacggc ccggcgacaa
    48721 tcccctgaat cacgcggaag aagatcagca tattcaggct gctggagaca ccacacgccc
    48781 acgacgcaat agcaaaggcg atggtggacc aaaggaacag tttcacttcc ccgacgcgct
    48841 ttgccagcca gccggtaagc gggatcgaga tggcattcgc caccccgaaa gaagtgatta
    48901 cccacgttcc ctggctgagc gatgagccca gattcccggc gatagtgggg atcgccacgt
    48961 tagcaatggt ggagtccagc acctgcatga atgtcgccag tgacagcgca atcgtcataa
    49021 tgaccagttg cgcgccttcc agcggttttt gctgttgcat cacacgcacc tctggattag
    49081 ccagcgttag cttttacgat atcgtcgatc agtttattga caggtgccag gctgatttca
    49141 cgcgcggtac ttaccgcgac cggtgttgaa cgtactttat ttgccagtac ctgaccgtca
    49201 cggttagtgg tattgacgct caccagtgtg gacaaaccaa tgcgcagcgg gtattgctcc
    49261 agctgtttct ggtccagttc gatacgcaca ggtagacgct gaacgacttt gatccagtta
    49321 ccggtcgcat tttgcgccgg aagcagtgag aatgcgctac ctgtgcccat atccagacca
    49381 accactttac cggtgtattt cacatcatcg ccgtaaatat ccgtggtgat agtgaccggc
    49441 tgaccgatac gcatattggc aatctgcgtc tctttaaagt tggcatccac ccacatattg
    49501 gtggctggaa cgaccgccat cagcggcgtc gttgggctaa tttgcgcccc aggctgtacc
    49561 gcacggcggg agacataacc ggtcatcgga ctgacaatac gggtacgctc cagcgccagc
    49621 caggcgttac gtacttcggt ggcagcctgt tgcacggcag gctgatcttc cagtttagtc
    49681 cccagaatca tcgcctgatt ggcattgtat tgttgaatag cgacgtccag ttgcgcctgg
    49741 gcactggtga cggcgtcgcg ggcgtgttgc agctcttcgc gaccaatcag gttggcattg
    49801 cccagcggca cacggcggtt gtagtcgctt tgtgcttttg cgagggcgat tttctgcacc
    49861 tcaatattcg cctgcaactg tttgctgtta atcatcagct ggtgggtttg gcgaacgctg
    49921 gaagccagtg cagttttggc tttttcaaac gcctggcgag catctgtcgg gtcgagagtg
    49981 accagcacgt cgccttcttt tacaaaatcg gtgttatcgg cccagacttt cgtcacgctg
    50041 ccagacacct gagacataat ttgaatctga ttccctgcca cgtatgcgtc atcggtttct
    50101 tcgaaatgac gcagtaccaa aaaccaataa atccctatcg ctacggcaat aattataaag
    50161 agcaaggtga gaaggaggag cagacgctta cgtttgccgc tcttctttac cggttgctgc
    50221 ggggtttgag tctccgcatt tgcgctcata ttgttctcca cgatcttctt aactcatcgg
    50281 ctgagccgac ctgcttgcca aaaaggccag catgttgcga tgctggccgg tcattttttc
    50341 ttttataaat ctggattttt gagcgagatg acgcgttagc tcatcgcttc gagaaccaca
    50401 ccgtcttgtt ccatctggtc gagacgggag agcaatttgc gggtgatttg ctcgagctga
    50461 tctttttctg ttgtgctgag cgcggaccag agttgatgca ggcagttatg ctgcggtggt
    50521 aaaacctcgc gcaaaaactc gtgacctttt tccgttaatt gcagatgcag gcagcggcga
    50581 tcgttatcgc tttcacgacg ttcgatccaa ccgcgttttt ccagttcatc ggcaatacgc
    50641 gtcgcgttgg tacgggatga tccaagagca caacttaatt cagaaggctg aatactgtgg
    50701 ttttcctgag actccagcgt aatcaacgcc ataaacaacg tctcgttaat cccctgagcc
    50761 ttcagcattt tattgcggtt ctccagcagc ttgctttgca tgtgcatgca aagacgagtc
    50821 agaaggatct cctgataagg aaaatcttcg tggcggctgg cgcgaaattt tagcatttgt
    50881 tcaatgggcg taaacgaact atccatttgg gtatgacctc attaatttca gccgatatag
    50941 taacgacagt gacaaataaa gtaaatgtat tgttttagaa aaatgattct tgtgggttat
    51001 ataatcgcca tcactttcca ggcgagccca taggccagcg cactaagcag tgttgggatg
    51061 ataatgctgc gtgttttata gaaactggca cccagtaccg cgaagccgac cagcgtgggc
    51121 acgaaacggc gtgtatcgtg catcacttct ggtgcggtag agacaaccag cagagcgcat
    51181 atcgaggcga tgccaatggt gtcgagcaaa atacctaccg cgccacgttt ggttgggcgg
    51241 gcattaccca cacgcaggcg cagcggcaaa tagcggaagc aataattcgc cgcgccaacc
    51301 agtaacccag gcagcagaac ctcatagctc atcgggcgct ccttgccaga atgcttggat
    51361 taacgcagtg aggcagccac agacaatgcc tgccagaatg gcgacgggaa tagaaaatag
    51421 cgttacgcct gcaagggcac caactaacgc tgcggtaacg caaagagatt gtttgcgctg
    51481 gaaagaggcg agcaggaaac tcataaagag tgccggaagc ataaaaccta atgctgcttc
    51541 aacggcggga taaccttgca gcaagccgct gccagagaat gcccctatta ccgtaccaaa
    51601 tacccacgat gaccatgaac tgaaggcaat gccgatcatc cagttctcgc tccagcggcg
    51661 attattgcgt accagttttg ctgttgcggc ggcaaaaacc tcatccgtca ggccaaacgc
    51721 ccacagggcg gttttcgatt tttgcagacg ctgaataata cggctacgca gtgacgggcc
    51781 atacaacaca tggcgaacat ccattgccat gacggtcagt gcagcaaccc acaaactact
    51841 cccggctgcc agcatcgcgg taatgacgaa ctggctcgcg cctgcataaa tgatgcagga
    51901 gaaaaaaacg ctttcgagag gagagaatcc cagacgggtc gcattcagac cgaacgcaaa
    51961 ggccaccgga atataactaa taacaatcgg taaactgtct ttgcatcctt ccatgaaggt
    52021 cgccgaacca ggagcaggct gtggagtagg gctttccata gagtgtacgc ttaacgattg
    52081 ttaatattat aaatagactg aatgaatatc ttaaccttat cagactgatg ggcttcttaa
    52141 cacccttata agtgtaaagc cacgaaaacg gttgctgatt gcttaatttg cggcttcctg
    52201 acgatgaaaa cctcgccacc agaccagtaa gttaactagg gcaatcgtcg cgccagccag
    52261 acaaacgcca gcccaaccgc catgttgcca ggctgaggct gaaattagcg aaccggcggc
    52321 accgccaata aagtagctgg tcatgtaacc tgcggtcagg cgattacgcg catcagggtg
    52381 tattcgataa attaccgtct ggttagtgat atgcacgccc tgtacggtga gatccagcac
    52441 caggatgccg ataatcagcg ccagtacgga agtgtgacca aaccagatcg ccagccatga
    52501 aagtaatagc agcagcagac cgaaagttgt ggtgtggtgc gatttaccct tatcggcaaa
    52561 accgcccgcc ggacgagcgc ccaacgctcc ggcagctccc gcaagtccaa acagaccaat
    52621 gacaccatcg ctgtagttaa aaggtggagc ggcaagcaaa aaggccattg aggtccagag
    52681 aatgctgaag ttggcaaagg tcaggcagcc cagcaatgcg cgggtgcgga ggatcttatc
    52741 gctgataaac atgctgaaaa cggaacctaa caactgtggg tagttgaggt gggtttctga
    52801 tttcatttgt ggcagaccac gccataatgc tagcgccatc agtgccatta acaccgaggc
    52861 aacccaaaag acggtgcgcc agccgccgag gtttgccagt aacccggcaa cggttcgtgc
    52921 cagcaagatc cccaacagca gaccgctcat aatggtgcca accactttgc cgcgtttatc
    52981 cggtgaagcc agcgtcgctg ccagcggaac cagaatttgt gccacgactg agaataaacc
    53041 ggttaatgcc gtaccgagga tcatcatcgc cagcgactga ctgctggcag tgattaacat
    53101 gccgcctgcc gccagtaagg tcatcgagac aatcaggcgg cggcgttcaa acatatcacc
    53161 gaggggaaca agaaacagca gacctgctgc atagcccaac tgcgcggcgg taacaataaa
    53221 gcctgccgaa ctggcggaaa gggaaaagtt acgcgcgatg gtgtcgagca atggctgggc
    53281 gtaatagtta ctggctaccg cctgaccggt ggcgatagac atcagcacga tcagcgccgg
    53341 gctaagctca tgattaggtt tagtcattat ttttgttcat caggtgatga ttaagctgga
    53401 aatcaataat agcggatgtt gatgaatggg ggcgaaagat agcttgttga aatgtgcgat
    53461 gtggtttgta ggcatgataa gacgcgccag cgtcgcatca ggcattgtgc acagaacggc
    53521 ggatgcggcg aaaacgcctt atccgccata cggaatgagt gtattttgta ggcatgataa
    53581 gacgcgccag cgtcgcatca ggcattgtgc acagaatggc ggatgaggca aaaacgactt
    53641 atccgcccga ataaaaatta cttctgcgct gccagcgcct cattcaccca gccatcgaac
    53701 tgctgctggt gggctttgat ccaaccatca acatggccct gaatatcgcc ttctgaggct
    53761 ttgccgtcat gcataatggc gttctgggcg ttaatatctg ccactggcaa ctgcataatg
    53821 gcaaaaagtt tcgctgctgc cgggtttttc tcggcccagg ctttgttggc aacgatatgc
    53881 atggtgctga ccgggaagcc ataattcgca ccattcggca gtttggtatc ggcgttttta
    53941 tcgcccggca gtgcggagaa cggcacctgc aaccagacca catctttccc tggcttcagc
    54001 tcattactca cccagtacgg cgtccaggtg taataaaaca ccggtttgcc ctctttgtag
    54061 cgactgatgg tgtcggccat catcgctgcg tagttcccct gattatgcgt cacggtgttg
    54121 gtcagttcat acgcggcaag ctggtggttg atcgcacctt cgcagcccca gcctgggtta
    54181 cagccggtta aatccgcttt accgtcgccg ttggtatcga acagtttggc gatcttcgga
    54241 tctttcagtt gtgcgatgtt ggtgattttg tactggtcgg cggttttctt atcgatcagg
    54301 taaccctgtg ccgcgccgtt aacgaatatc ccttcacaat aaaatttctt atcgccaccg
    54361 gcagcttcgt acatgttgtc atgcagcggc gtccagttca cggcggtgaa ggttgcatcg
    54421 ccggaagcaa gtgaggtgta gccaacgttg taatccactt cgctgggttt attgacggta
    54481 taacctaatt tctccagtgc acgactgacc agcagcgtct ggaaggtttc ttcagtgatg
    54541 gtgctctgaa ctggattaac agtaatgcct ttgcccggca gatcggctgc aaaagtttgt
    54601 gtagagataa gcgtggcaaa cgctgtcgca aaaagtacgc tatgtcgcat tgttattcct
    54661 ttttttggca gggtgataat gcccggcgtt accgggcaag tgcagagtta cttaatgaat
    54721 gggcgggtca gcagaccgac agggccagtg gtgtaccagc gacggttgcc gcgactgcgt
    54781 gagtcgcgcc caacggcctg cgtcaggcgg tcgaggataa tggcgaggat cacaatcccg
    54841 acgccgccaa cggtggcaag gcccatatcc agacgaccga taccgcgaag taccatctga
    54901 cccagcccgc cgacggcaat catcgaggcg atgaccacca tagaaagagc cagcatcagc
    54961 gtctggttaa cgcccgccat aatggtcggc atcgccagcg gtaactgaac tttgaacagc
    55021 atctggcgcg ggctggcacc gaatgagcgc gaggcttcaa tcagatccgc cggaacctgg
    55081 ttaatcccca gaatcgtcag acggataatc ggcggcagag caaagatgat cgtcaccact
    55141 acgcccggca cgttaccgat accaaacagc atgacgattg gtaccagata aacaaacgct
    55201 ggcgtggtct gcatggcatc aagcagtgga cgaataattt tcgccgctcg cggacttctc
    55261 gccagccata tccccaacgg caaaccgatg acgatacaga acagcagggc ggttaacacc
    55321 agcgccagag tcaccattgc ctgcgaccag gcaccgattg cgccgatggc aatcagcgaa
    55381 accagcgtcg ccacgcccat tccgaccccg gaaatctgcc aggcgatgag agcgaaaacg
    55441 ataatcgcca ctggcgctgg catacccagc agcaattgct ggaaactgtt gaggatataa
    55501 tcaaccggaa cgcgcacgcc ctggaagacg ggacggaaat gggtaacaac ccagtcgatc
    55561 ccttcagtga cccaactgtc gagcgggatc agcgttttat ggaacggatc gagaatatta
    55621 aaatgctcga cgtttggcgc aggcgtactg gtcagccagt cagcaccacc gccgtcagtc
    55681 ggtgcagtcg tcggtgtacc ccaggcgtct gcggattgcg cggcactgtc cgccgctggc
    55741 gtggtatccc acggattatt ttgatcagcc attatttacc ccctcacgat ctaaagcgcg
    55801 cagcagcatt cctttcgaaa tgatgccgac atactgttgg tcctcgtcga ccacgggcac
    55861 cgcacagggt gcctgtccga catgagagag caactcgcta agaggcgttt gtgcatcgac
    55921 tgctaacggc gcatcaatca gcgccgcatc aagaccttgc tgctgcgtta acgcggtttt
    55981 aagcgaatcg atggagactg cgccgacaaa cttattaccg cgttcgataa cgtagccata
    56041 ttcgcgatct tcatcctgca ataatttcag tgccgaacgt gggccgaagc caggggtttt
    56101 acgaattaag ccattcggtg tccggcgggc aatatctttc gcactgaata cctgactaat
    56161 atcaacgcca cggaagaagg tacggacata atcattcgcc ggattattga gaatttcatc
    56221 cggtgtgccg acctgtacca cttcaccatt ttgcataatg gcaattcggt cgccaatacg
    56281 catggcttca tcgagatcgt gggaaataaa gacaatggtg cgctgatgtt tcgcctgtaa
    56341 ttttaccagc tcatcctgca tctcggtgcg aattaatgga tcgagcgccg agaaggcttc
    56401 gtccattaat aaaatatccg gattaatcgc taacgcgcgg gctaatccca cacgttgacg
    56461 catcccgcca gagagttcat ccgggtagct gtgggcataa ttttccagcc cgacctgacg
    56521 cagtgcatca agggcttttt cccggcgttc ttcggcatta attccggcca attccatacc
    56581 gaacgcagta ttgtccagca cggtcatatg cggcattaag gcaaaggact ggaagaccat
    56641 cgcaatcttt tttctgcgca cctcacggag tttggcatca gatattttgg caatatccac
    56701 accatcaatc agcacttgcc cgcgggtggg ttcaatcagg cgattgagaa ggcgtaccat
    56761 tgtggattta cccgagccgg ataatcccat gatgacaaat atctcgcctt cttcaatggc
    56821 cagactggcg tcttttacgc caagcgatag cccagttttt tccagaattt gttcttttga
    56881 aagtccttgt tcgatatatt tgaatgctcg ctgtggatgc tcaccaaata ttttataaag
    56941 gtttttaatt tctaatttaa ttgccatgca atagaaagat tcctgttatt tgtttatgtc
    57001 gatatattac ccaatggaaa tagttacttt tttctaccct aacatactga gaatctgagg
    57061 caacccctga tggcaaatgt ggcatgaata ttgatgcgag caaatcgccg gaatttcccg
    57121 tgatataagg gctgagagca aatcgagaaa agttgatatt ttttgtcaaa gatagcgaaa
    57181 aaacgcctga atattgtggt ttcaggcgaa taatgcaaca gagtatcagt gtagatcacc
    57241 acaaaatatt tgcgtgataa aaagctattt ggcgggaatt atttccctgt cgcgggtagt
    57301 gatatttttg aaaataacac cctcagaaat tccagtcttc atcttctgtt tcaaccgctt
    57361 tccccatcac ataagaggag cctgaaccgg aaaaaaaatc gtgattttca tcggcattcg
    57421 gcgaaagcgc ggcgaggatt gccggattca cttccgccat ttctgcggga aataaaggtt
    57481 cgtagcccag attcatcaaa gccttattgg cgttgtaaca gagaaacgct ttcacatcgt
    57541 cagcccacgg ggtttcggcg tacagctcat cggtgtattg cagctcgttg tcgtagagtt
    57601 ccagcaacaa atcgaaggcg aaactcttca actcttcacg ttgtcccaga gatatctttt
    57661 ccatgttttt ctgatattta tagcctatgt agtaaccgtg gactgcttca tcacggataa
    57721 tcagacggat caggtctgcg gtattggtca gctttccgcg gctggaaaaa tacatcggca
    57781 gccagaaacc ggaatagaac aaaaaagatt caagaaacac actggcgatt ttctttttca
    57841 gcggatcatc accgcgataa tgttgctgaa taatctgagc ttttcgctgc aacggtgcgt
    57901 tttcttcact ccaggcgtag gcggcatcga catctttggt ctggcacagc gtcgagaaaa
    57961 tcgaactgta agagcgggca tgaaccgctt ccataaagct gatattcgat aataccgctt
    58021 cttcatgagg cgtgagtgca tcgggcatca gcgaaggcgc gccgataaca ttttgcagcg
    58081 tgtcgagcag cgtcaggcca gtaaaaacgc gcatcgtcag ttgttgttct acgacagtta
    58141 atgtctgcca ggcaggaata tcgttcgaca gcggcacctt ttctggtagc cagaaattgc
    58201 tggtcaggcg attccacacc tccagatctt tatcgtcaga tatcttgttc cagttgatgg
    58261 cgctgatacg tgagagtttc atagatattc cttaaagtgc acaggagacg cagccttcaa
    58321 tttcagtgcc ttccagcgcc atctgacgca ggcgaatgta atagagcgtt ttgatacctt
    58381 tgcgccaggc gtaaatctgc gctttgttga tatcgcgagt ggtggcggta tcggggaaaa
    58441 acagcgtcag cgacaaaccc tgatcgacat ggcgagtcgc ttccgcgtag gtgtcgatga
    58501 tcttttctgc gccgatttcg taagcatcct gatacagcgc cagattttcg ttagtcataa
    58561 acggggcagg gtagtaaacg cgtcctgttt tgccctcttt gcgtatctct actttcgcca
    58621 caatcggatg aatactcgac gtagcatggt tgatataaga gatagaaccg gttggcggca
    58681 ccgcctgaag attctggttg taaatgccgt agcgcatcac gtcgtcgcgc agttgcgccc
    58741 acatctcacg ggtaggtaac gtaataccgc tgcgggcaaa cagttcgcca actttcgccg
    58801 ttttcggctg ccagttacct tgcagatatt ggctaaaata ttcaccactg gcataacgtg
    58861 actgtttgaa cccggcgaag gtttcaccgc gttcgcgcgc cagcaacatc gaggtacgca
    58921 gtgcgtgcca ggtgatggca tagaaataga gattggtgaa atccagtgct tccggcgaac
    58981 cataagcgat gccttctcgc gccagatagc cgtgtaaatt catctgcccc agtccgatgg
    59041 cgtgtgaggc ggcatttccg gcttcgatgg acggcacgct gcggatatga ctcatatctg
    59101 acaccgccgt taaaccgcgc acggcagtct ctaccgtgcg ggcaaaatcg ggggaatcca
    59161 tggtgtgcgc aatattcaac gaacctaaat tgcaggagat atcatggcct gtgcgggcat
    59221 agtcgagatt ctcgtcatac tctgaggcac agttgacctg caaaatctct gagcagagat
    59281 tactcatatt tatgcgcccg gcgacagggt tagcacggtt taccgtgtct tcatacatga
    59341 tgtagggata gccggactca aactggattt ctgccagccg ctggaagaaa tcacgggcgt
    59401 tgaggtattt tttgcggatg cgttcatcgg caaccagttc gtcatagtgt tcgctgatgg
    59461 cgatatcggc aaacggcttg ccataaactc gctcaacgtc atagggcgaa aacagcgcca
    59521 tctgcgcatt ctcttttgcc agatggaaag tgatatccgg gatcaccacg cccagcgaca
    59581 gtgttttaat gcggattttt tcgtcggcat tttcccgttt tgtatcgaga aaacgcagaa
    59641 tatcgggatg atgagcatgt aaatagactg cacctgcccc ctgacgcgcg ccgagttggt
    59701 tggcatagga aaatgcgtct tccagcattt tcatcaccgg aattacgcca gaagattgat
    59761 tttcaatacg tttaattggc gcgcccgctt ctcgcagatt cgacagcaaa aatgccacgc
    59821 cgccgccgcg tttcgacagt tgcagtgcgg aatttaccgc ccggccaatc gactccatat
    59881 tgtcttcaat acgcagcaga aaacaggaaa ccagttcgcc gcgctgctgc ttaccgcagt
    59941 tgaggaatgt cggcgtggct ggctgaaagc gtcctgacag catttcattg gtcagttgca
    60001 acgccagcgt cgcatcgcct tgtgccagcg tcagcgccac catcgtgact cgatcggcaa
    60061 aatcttccag ataacgttta ccgtcgaatg tcttcaacgt atagctggtg taaaacttcc
    60121 atgccccgag gaatgtctgg aaacgaaaac cgctggtgtg tgcgtgggca aacagcgtaa
    60181 tgacaaagtc gcgagagtag cgattaagaa cgctttcatc atagtaacct tcgttgacca
    60241 gccagttcag gcgctgctgc tggctactga aggtcacact gttcggacgc acatgcgtcg
    60301 ccataaaggc gtcaacggcc tggcggtctt tatcgaactg aatgcgacct gcgctatcgt
    60361 agaggttaag catcgcattc agcgcgtggt aatccatcgt ttcctgcgtc aggcattctg
    60421 cggttgtcgt tgccaaaatt cggttactcc tttacgaacg ttttcgatat cgctttgcgt
    60481 acccatgagt tcaaaacggt acagccacgg cacgccgcat ttccgggcaa tcacatctcc
    60541 ggcgcggcca tacgcctcac caaagttgcg attaccagaa gcaataacgc cgcgaagcaa
    60601 cgcccggttg tgctcgtcgt ttaaaaagcg aattacctat cgtggcaccg cgccagccgt
    60661 accgccgccg ccgtaagagg gcacgatcag gatgtaaggc tcgtctacct gaatccgttc
    60721 ccgctcattg agcgggatgc gcaccgcggg cagacctaaa cgttcgataa aacgctgcgt
    60781 gttttcggag ctgctggaga agtagacgag ctggctcatg cactggccgc gtgtggcgct
    60841 ggatgcagac ggttaatcat gtccggacgg aaaccagacc agctaagatc gccagcaatc
    60901 actaccggca actgacgaaa gccctgagca cgcaacgctt ccgctgcttc aggaacgcga
    60961 tcgacattaa tcatttcaaa atcaaagccc cggttttcca tcgcccgttt ggtggcgtgg
    61021 cactgaacgc aatcgttacg agtgtaaata gttatgcgca tgattcgtat ttccgtttaa
    61081 aatgaagata cggcgcgatg atacgcgtcg ggttgtctct ctgttgatac agagatacta
    61141 gatgtagttg aaaaaagatt caaccacaca atatatagca aatagtgatc gaaattaccc
    61201 tagatatgag cgtgacgggg gagggggatt tttgtgattc accaggcaaa aagaaacccc
    61261 gaagacaggc ttcggggtta aagacgcgta tttattatca tttttgcact acgatttgcg
    61321 catgcttaac agtgcgccga taaaaatacc taccgcagct gctgtaccca cgctacacca
    61381 gggtctttca cgaacaaaag aatccgcgca gccaacggca tcgcgtgcgg cttgctggac
    61441 gcgagtacga ccatgcattc gcgccctggt ttctttcagc aatgcctgag ctttactgcg
    61501 tgcggcttca gcttcccctt tggcgtcgct gccccaggac ttcaatacag attcgaggct
    61561 gtcagctaat tgattgacat cattctggat atcctgcacg ccatcatcaa cgtcgttgcg
    61621 gttcggtcgg ttaaacatat gatcccccat tagaattcga tgtaattttc agtgtagacc
    61681 ataattttct taactgaggt tcatcgcggc tttatggact tttctgaaag cgttgtgaat
    61741 gcgcaatcag gtaaggtagg cccgcggttg ataataacga ggatcaaata tgtatttacg
    61801 accagacgag gtggcgcgcg tacttgaaaa agtcggtttt actgtcgatg tggtaaccca
    61861 gaaagcttat ggttaccgcc gtggggaaaa ttatgtctat gttaatcgcg aagcgcgaat
    61921 gggacgtacc gcactggtaa ttcatccaac attaaaagaa cgtagttcga cactggctga
    61981 acccgcttcc gatattaaaa cttgcgatca ttatcaacaa tttccgctct atttagcagg
    62041 cgagcgacac gagcattacg gtatcccgca tggcttcagt tcgcgtgttg cgcttgaacg
    62101 ttatttgaat ggtttatttg gcgaagccag ttaaagacgc gactggcgat gccagtcgcg
    62161 aaaagaagag tcaggctttt acctgctggt aatggctgac tttaaacagt cggcggcaat
    62221 aatcgaggaa gtagccataa accgccccca tcaacatcga aacaacgatg tttgaactga
    62281 ccgccgccat aatctgatgc cagtctgcgc ccaccactaa caagatcgcc acatacaccg
    62341 gtgactggaa cgtcacataa gccaggatat cagccagatt ttttatccag cccgacgggc
    62401 taactttgcg tgccgcgcgc ataaatagat cgcggtacat accgtatggc catgcaatta
    62461 agatgttcac cggaatcgct accaatctgg aataaaaaga ctgttcgaag ctcattccgg
    62521 agaggaaaac ttcaatacac atgttcacga cagaacagta aacaaccatc gcgaacgtat
    62581 ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat gctaatgctc cttaataaaa
    62641 gcgtaatggg atgttaatgg agatgaaagc agtggatctc attgccatct gcattttcgc
    62701 atagcgtata tcgctggcta tgactattca attagtgaaa aatttttatt tagtcagttt
    62761 ttgtagataa aatattccgt aacggctcgt tttttgtaca ttgtggattt ttacatgctg
    62821 atatgattta ttttcaatta aatcaattag atatgacctt gcctttaatt tcactaatga
    62881 aagtttgggg gtgaggtaac gctataagcg tagagaattc ggtgtatgtc gtgaccgtgt
    62941 catttacagc atttttgatg ccagaaaatg tacattaaca gcagaaagca ccagtgaggg
    63001 ggg
//
LOCUS       DAZPJR010000005        58919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPJR010000005 DAZPJR010000000
VERSION     DAZPJR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.006"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(173..1120)
                     /gene="dusC"
                     /locus_tag="ACHVB7_000327"
     CDS             complement(173..1120)
                     /gene="dusC"
                     /locus_tag="ACHVB7_000327"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR1912428.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1359..1757
                     /locus_tag="ACHVB7_000328"
     CDS             1359..1757
                     /locus_tag="ACHVB7_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR1912429.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1754..2449
                     /locus_tag="ACHVB7_000329"
     CDS             1754..2449
                     /locus_tag="ACHVB7_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR1912430.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2579..3463
                     /gene="cdd"
                     /locus_tag="ACHVB7_000330"
     CDS             2579..3463
                     /gene="cdd"
                     /locus_tag="ACHVB7_000330"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR1912431.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3613..4332
                     /gene="sanA"
                     /locus_tag="ACHVB7_000331"
     CDS             3613..4332
                     /gene="sanA"
                     /locus_tag="ACHVB7_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR1912432.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4335..4574
                     /locus_tag="ACHVB7_000332"
     CDS             4335..4574
                     /locus_tag="ACHVB7_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR1912433.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4768..6006
                     /gene="preT"
                     /locus_tag="ACHVB7_000333"
                     /pseudo
     CDS             4768..6006
                     /gene="preT"
                     /locus_tag="ACHVB7_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            6000..7235
                     /gene="preA"
                     /locus_tag="ACHVB7_000334"
     CDS             6000..7235
                     /gene="preA"
                     /locus_tag="ACHVB7_000334"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR1912434.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7362..8372)
                     /gene="mglC"
                     /locus_tag="ACHVB7_000335"
     CDS             complement(7362..8372)
                     /gene="mglC"
                     /locus_tag="ACHVB7_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR1912435.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8388..9908)
                     /gene="mglA"
                     /locus_tag="ACHVB7_000336"
     CDS             complement(8388..9908)
                     /gene="mglA"
                     /locus_tag="ACHVB7_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR1912436.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9969..10967)
                     /gene="mglB"
                     /locus_tag="ACHVB7_000337"
     CDS             complement(9969..10967)
                     /gene="mglB"
                     /locus_tag="ACHVB7_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR1912437.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11247..12245)
                     /gene="galS"
                     /locus_tag="ACHVB7_000338"
     CDS             complement(11247..12245)
                     /gene="galS"
                     /locus_tag="ACHVB7_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR1912438.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            complement(12429..13586)
                     /gene="yeiB"
                     /locus_tag="ACHVB7_000339"
     CDS             complement(12429..13586)
                     /gene="yeiB"
                     /locus_tag="ACHVB7_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR1912439.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13603..14271)
                     /gene="folE"
                     /locus_tag="ACHVB7_000340"
     CDS             complement(13603..14271)
                     /gene="folE"
                     /locus_tag="ACHVB7_000340"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR1912440.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14529..15365
                     /gene="yeiG"
                     /locus_tag="ACHVB7_000341"
     CDS             14529..15365
                     /gene="yeiG"
                     /locus_tag="ACHVB7_000341"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR1912441.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15397..17388)
                     /gene="cirA"
                     /locus_tag="ACHVB7_000342"
     CDS             complement(15397..17388)
                     /gene="cirA"
                     /locus_tag="ACHVB7_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR1912442.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17680..19149)
                     /gene="lysP"
                     /locus_tag="ACHVB7_000343"
     CDS             complement(17680..19149)
                     /gene="lysP"
                     /locus_tag="ACHVB7_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR1912443.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19354..20235)
                     /gene="yieE"
                     /locus_tag="ACHVB7_000344"
     CDS             complement(19354..20235)
                     /gene="yieE"
                     /locus_tag="ACHVB7_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR1912444.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20334..21383
                     /locus_tag="ACHVB7_000345"
     CDS             20334..21383
                     /locus_tag="ACHVB7_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR1912445.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21457..22314
                     /gene="nfo"
                     /locus_tag="ACHVB7_000346"
     CDS             21457..22314
                     /gene="nfo"
                     /locus_tag="ACHVB7_000346"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR1912446.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22318..23406
                     /locus_tag="ACHVB7_000347"
     CDS             22318..23406
                     /locus_tag="ACHVB7_000347"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1912447.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23462..24712)
                     /locus_tag="ACHVB7_000348"
     CDS             complement(23462..24712)
                     /locus_tag="ACHVB7_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1912448.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24812..25753)
                     /gene="rihB"
                     /locus_tag="ACHVB7_000349"
     CDS             complement(24812..25753)
                     /gene="rihB"
                     /locus_tag="ACHVB7_000349"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR1912449.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25883..26581
                     /locus_tag="ACHVB7_000350"
     CDS             25883..26581
                     /locus_tag="ACHVB7_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR1912450.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLLDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26648..27898)
                     /locus_tag="ACHVB7_000351"
     CDS             complement(26648..27898)
                     /locus_tag="ACHVB7_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1912451.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27992..28930)
                     /gene="psuG"
                     /locus_tag="ACHVB7_000352"
     CDS             complement(27992..28930)
                     /gene="psuG"
                     /locus_tag="ACHVB7_000352"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR1912452.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28918..29859)
                     /locus_tag="ACHVB7_000353"
     CDS             complement(28918..29859)
                     /locus_tag="ACHVB7_000353"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR1912453.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30317..32008)
                     /gene="fruA"
                     /locus_tag="ACHVB7_000354"
     CDS             complement(30317..32008)
                     /gene="fruA"
                     /locus_tag="ACHVB7_000354"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR1912454.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(32025..32963)
                     /gene="fruK"
                     /locus_tag="ACHVB7_000355"
     CDS             complement(32025..32963)
                     /gene="fruK"
                     /locus_tag="ACHVB7_000355"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR1912455.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32963..34093)
                     /gene="fruB"
                     /locus_tag="ACHVB7_000356"
     CDS             complement(32963..34093)
                     /gene="fruB"
                     /locus_tag="ACHVB7_000356"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR1912456.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34461..35642
                     /gene="setB"
                     /locus_tag="ACHVB7_000357"
     CDS             34461..35642
                     /gene="setB"
                     /locus_tag="ACHVB7_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR1912457.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35639..35893)
                     /locus_tag="ACHVB7_000358"
     CDS             complement(35639..35893)
                     /locus_tag="ACHVB7_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR1912458.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36048..36620
                     /gene="yeiP"
                     /locus_tag="ACHVB7_000359"
     CDS             36048..36620
                     /gene="yeiP"
                     /locus_tag="ACHVB7_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR1912459.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36843..38309
                     /locus_tag="ACHVB7_000360"
     CDS             36843..38309
                     /locus_tag="ACHVB7_000360"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1912460.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38427..39413
                     /gene="yeiR"
                     /locus_tag="ACHVB7_000361"
     CDS             38427..39413
                     /gene="yeiR"
                     /locus_tag="ACHVB7_000361"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR1912461.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39452..40165
                     /gene="lpxT"
                     /locus_tag="ACHVB7_000362"
     CDS             39452..40165
                     /gene="lpxT"
                     /locus_tag="ACHVB7_000362"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR1912462.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40577..41143
                     /gene="mepS"
                     /locus_tag="ACHVB7_000363"
     CDS             40577..41143
                     /gene="mepS"
                     /locus_tag="ACHVB7_000363"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR1912463.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41324..42880
                     /locus_tag="ACHVB7_000364"
     CDS             41324..42880
                     /locus_tag="ACHVB7_000364"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR1912464.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42962..44776
                     /locus_tag="ACHVB7_000365"
     CDS             42962..44776
                     /locus_tag="ACHVB7_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR1912465.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44777..45871
                     /locus_tag="ACHVB7_000366"
     CDS             44777..45871
                     /locus_tag="ACHVB7_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR1912466.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45871..46896
                     /locus_tag="ACHVB7_000367"
     CDS             45871..46896
                     /locus_tag="ACHVB7_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1912467.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46898..48487
                     /gene="yejF"
                     /locus_tag="ACHVB7_000368"
     CDS             46898..48487
                     /gene="yejF"
                     /locus_tag="ACHVB7_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR1912468.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48491..48835)
                     /locus_tag="ACHVB7_000369"
     CDS             complement(48491..48835)
                     /locus_tag="ACHVB7_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR1912469.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49168..50225)
                     /locus_tag="ACHVB7_000370"
                     /pseudo
     CDS             complement(49168..50225)
                     /locus_tag="ACHVB7_000370"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(50316..51011)
                     /gene="rsuA"
                     /locus_tag="ACHVB7_000371"
     CDS             complement(50316..51011)
                     /gene="rsuA"
                     /locus_tag="ACHVB7_000371"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR1912470.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51161..52921
                     /locus_tag="ACHVB7_000372"
     CDS             51161..52921
                     /locus_tag="ACHVB7_000372"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1912471.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            53046..53330
                     /gene="rplY"
                     /locus_tag="ACHVB7_000373"
     CDS             53046..53330
                     /gene="rplY"
                     /locus_tag="ACHVB7_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR1912472.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53469..54476)
                     /gene="yejK"
                     /locus_tag="ACHVB7_000374"
     CDS             complement(53469..54476)
                     /gene="yejK"
                     /locus_tag="ACHVB7_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR1912473.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54658..54885
                     /locus_tag="ACHVB7_000375"
     CDS             54658..54885
                     /locus_tag="ACHVB7_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR1912474.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54905..56665
                     /gene="yejM"
                     /locus_tag="ACHVB7_000376"
     CDS             54905..56665
                     /gene="yejM"
                     /locus_tag="ACHVB7_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR1912475.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56740..56816
                     /locus_tag="ACHVB7_000377"
     tRNA            56740..56816
                     /locus_tag="ACHVB7_000377"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56774..56776,aa:Pro,seq:ggg)
     gene            complement(56919..58919)
                     /gene="yejO"
                     /locus_tag="ACHVB7_000378"
                     /pseudo
     CDS             complement(56919..58919)
                     /gene="yejO"
                     /locus_tag="ACHVB7_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        14506 a        15372 c        14386 g        14655 t
ORIGIN      
        1 ggctaccggg tcgggcgcga ggtaacatcc ctgtaaaacg cgccctcagc ccacctccat
       61 gtgggctgcc ccggccttca gggaacgcct cggcaatttt gacgccacca aacaaccgtg
      121 cggcctattg ataaagagct aacacattgt caaaaaacat cactatggtt ttttagagtt
      181 tctcgatatc aattgcctga atagcccttg caatatcagg ggaattattc aacacccgaa
      241 catgctgaaa taattccgtt gcttcatcgt attctttacg caaataactc aaccactgtt
      301 taatccgcgc aacgtgatat aacccggtat cgccctgctt ttccagacgg gtatattttt
      361 gcagcaaagc aaccacctcc ggccacggca ttcgcggttc gttatatttt actacccggc
      421 tcaggttggg aatattgagc gccccgcgac caatcatcac tgcgtcgcag ccgctgatcg
      481 caatgcattg ttgcgcgctc tgccagtccc agatttcgcc gttggcaatc accggaatat
      541 tcagccgctg gcgaatctcg ccaatcgcct gccagtcaat atgctccgcg cggtaaccct
      601 gctcttttgt ccgtccatgc accaccagct ccgtagcacc agcctgttga accgcatcgg
      661 cgatttcaaa tttcttctca ccgctgtccc agcccagacg cactttcacg ctgacgggca
      721 aatgcgccgg tacagcttca cgcatcgctt ttgcaccctg gtagatgagt tcaggatctt
      781 tgagtaacgt cgccccgccg ccgctgccgt taaccgtttt cgacgggcag ccgcaattga
      841 gatccacgcc ccaggaacct aattccaccg cacgggcggc gttctctgcc agccattgtg
      901 ggaactgacc taacaactga acgcgcacca gcgtaccgga tggtgtccgg ctggcgtttt
      961 gtagctcagg gcaaatgcga tgaaagactt ttaccggcag cagttgatcc accacgcgga
     1021 caaactcggt gatgcacaga tcgtagtcgt taacttcggt cagcaattct cgcaccagag
     1081 agtcaagcac accctccatc ggagccagta acacacgcat atcttcaccc ggaaaaaaat
     1141 gaggcgctat gttagcgcct atggtcaacg gattaaaggt ctgcaaagag ggattgctgc
     1201 aaactgtaat ccctctctat gcttgatgta tgcctgagtt cgttgcgcat tgcgtaatgc
     1261 cggggagcga aaaaataagc gctgatttct taatgtgatc ggtagcacgt tttacgaatt
     1321 aattgtatga tgaatccatc tcatctgggg tgttgattat gagtaagaca ctgaacatta
     1381 tctggcaata tttacgcgct ttcgtcctga tttatgcctg cctgtatgca ggcattttca
     1441 ttgcttccct gctaccggta accattccgg gcagcatcat cgggatgctg atcctgtttg
     1501 tcctgctggc cttgcaaatt cttccggcaa aatgggtcaa tccggggtgc tacgtactga
     1561 ttcgctatat ggcgctattg tttgtgccga ttggcgtagg cgtcatgcaa tattttgatt
     1621 tgctccgcgc acagtttggc ccggtagtgg tttcctgtgc agtcagtacg ctggtggttt
     1681 ttctggtggt gagctggagt tcacaactgg tacacggtga acgtaaagtc gtaggtcaga
     1741 aaggatcaga agaatgatgg cgaatatctg gtggtcatta ccgctgacgt tgattgtctt
     1801 ttttgccgcc cgcaaactgg cggcacggta taagtttccc ctgcttaacc cgttgctggt
     1861 agcaatggtg gtgatcattc cttttttaat gctgaccggc atctcttacg acagctactt
     1921 taaaggtagc gaagtgctaa acgatctgct gcaaccggcg gtcgtcgcgc tggcctatcc
     1981 tttatatgag cagctacacc agatccgcgc gcgctggaaa tcgatcatca ccatctgttt
     2041 tatcggcagc gtggttgcga tggtgacggg gacttccgtg gcattgttga tgggcgcttc
     2101 accggaaatc gccgcgtcaa tcctgccgaa atcagtcacc acgcctattg caatggcggt
     2161 tggcggcagt attggcggta ttccggcaat cagcgcagtt tgcgtgattt tcgtcggcat
     2221 cctcggcgcg gtatttggcc atacattgct taatgcgatg cgtattcgta ccaaagctgc
     2281 gcgcggtctg gcaatgggga cagcctcgca cgccctcggt acggcgcgct gcgccgagct
     2341 ggattatcag gaaggtgcat ttagttcgct ggcgctggtg ttatgcggga taattacttc
     2401 gctgatcgca ccgttccttt tcccgattat tctggcagta atgggctaaa atttgcgatg
     2461 cgtcgcgcat ttttgatgta agtttcacgc gttgcataat taatgagatt cagatcacat
     2521 ataaagccat aacgggttcg taaactgtta tcccattaca tgattatgag gcaacgccat
     2581 gcatccacgt tttcaaaccg cttttgccca acttgcggat aacttgcaat ctgcactgga
     2641 acctattctg gcagacaagt acttccccgc tttgttgacc ggggagcaag tctcatcgct
     2701 gaagagcgca acggggctgg acgaagacgc gctggcattc gcactacttc cactggcggc
     2761 ggcctgtgcg cgtacgccat tgtcgaattt taatgttggc gcaattgcgc gcggtgtgag
     2821 cggaacctgg tatttcggtg ccaatatgga atttattggt gcaacaatgc agcaaaccgt
     2881 tcatgccgaa caaagcgcga tcagccacgc ctggttgagt ggtgaaaaag cgcttgcagc
     2941 catcaccgtt aactatacgc cttgtggtca ctgccgtcag tttatgaatg aactgaacag
     3001 cggtctggat ctgcgtattc atctgccggg ccgcgaggca cacgcgctgc gtgactatct
     3061 gccggatgcc tttgggccga aagatctgga gattaaaacg ctgctgatgg acgaacagga
     3121 tcacggctat gcgctgacgg gtgatgcgct ttctcaggca gcgattgcgg cggcaaaccg
     3181 ttcgcacatg ccttacagta agtcgccaag cggtgtcgcg ctggaatgta aagacggtcg
     3241 tattttcagc ggcagctacg ctgaaaacgc cgcattcaac ccgactctgc caccgttgca
     3301 gggggcgtta attctgttga atctcaaggg ttatgattac ccggatatcc agcgcgcggt
     3361 tctggcagaa aaagccgatg cgccgttgat tcagtgggat gccacctccg caacgctgaa
     3421 agctctcggc tgtcacagta tcgaccgcgt gcttctcgct taagcctggt gccggatgcg
     3481 gcgtgaacgc cttgtccggc ttgccagccc tctcttggtg tcgaaattcc cggcaaacag
     3541 tttgccgttt cttgcgcaaa accagcgggt aaagtagcct gatggaaatt ttccttagat
     3601 cgagtctcct gcatgttaaa gcgcgtgttc ctcagcctgt tagtcctgat cggcttgctg
     3661 ctgttgactg tgctcggcct cgatcgctgg atgagctgga aaaccgcgcc ttatatctac
     3721 gacgaattgc aggatctccc ctaccgccag gtcggcgtgg tgctcggcac agcaaaatat
     3781 tatcgtactg gcgtaattaa tcagtattat cgctaccgca ttcaaggagc gattaatgcc
     3841 tataacagcg gtaaggtaaa ttatctatta ctgagcggcg ataacgcatt gcaaagttat
     3901 aatgagccga tgaccatgcg caaagattta atcgctgctg gtgtcgaccc atcagatatt
     3961 gttctcgatt acgcaggctt tcgtacgctg gattccatcg tgcgtacacg caaagtgttc
     4021 gatactaatg atttcattat tatcacccaa cgtttccact gtgagcgggc attatttatt
     4081 gcgctgcata tggggattca ggctcagtgt tatgccgtac cgtcaccgaa agatatgctg
     4141 tcagtacgta ttcgcgaatt tgccgcccgt ttcggtgcgc tggctgacct ttatattttt
     4201 agacgtgaac cgcgtttttt agggccgctg gtccctattc cggctatgca ccaggtgccg
     4261 gaagatgcgc aggggtatcc cgccgtcaca cccgaacagt tacttgaatt acaaaagaaa
     4321 caaggaaagt agttatggat gtacagcagt tttttgtcgt tgccgttttt ttccttatcc
     4381 cgatattttg tttccgcgaa gcatggaaag gctggcgcgc aggcgcgatt gataaacggg
     4441 ttaaaaatgc accggaaccg gtgtatgtct ggcgagcaaa aaatcccgga ctctttttcg
     4501 cttatatggt ggcatatatc ggcttcggaa ttttatctat cggcatgatt gtttatctta
     4561 ttttctatcg ttaatatttt ctcttttctg ttaattattg agaataatta ttacttcact
     4621 caataagctg cggatataac attctgaacc gcagctattt gtgaatcctt tcacagttta
     4681 aatttcccca cacgcttagc cttaatatca gcacgcaata tttatttagc gttcgcctta
     4741 attactcata gcattaagga agatcacatg ccgcaacaaa attatctgga tgaactcact
     4801 ccggctttta cgcctttact ggcgattaaa gaagcctctc gctgtttatt atgtcatgac
     4861 gctccctgta gtcaggcttg cccggcacag accgatccgg ggaaatttat tcgctcaatc
     4921 tactttcgta attttaaagg cgctgccgag acaattcgcg aaaataatgc cctcggtgcc
     4981 gtttgtgcca gagtgtgccc gacggagaaa ttatgccaaa gcggttgtac ccgtgccggt
     5041 gttgatgctc ccattgatat cggccgctta cagcgttttg ttactgattt tgaacaacaa
     5101 accgggatgg aaatttatca gcccggtact aaaacgctcg gcaaagtcgc gattattggc
     5161 gcaggtcctg ccggattgca ggccagtgtg acactgacaa accagggtta tgacgtcacg
     5221 atctatgaga aagaagcaca acccggtggc tgattgcgta acggcattcc gcaattccgg
     5281 ttaccgcaat cagtactgga tgcagagatc gcccgtattg aaaaaatggg cgtgaccatt
     5341 aagtgcaaca acgaagtcgg taacacactc acccttgagc agctgaaagc agaaaaccgc
     5401 gcggtactgg tcaccgtggg gttatcaagc ggttccgggc taccgctgtt tgagcatagt
     5461 gacgttgaga ttgccgtcga cttcttgcaa cgtgcacgac aggcgcaagg cgatatcagc
     5521 attccacaaa gcgcattaat tatcggcggc ggtgatgtcg cgatggacgt agccagcacg
     5581 ctgaaagttc tcggctgtca ggcggtaact tgcgtagcgc gtgaagagtt agatgagttt
     5641 ccggcaagcg aaaaagagtt taccagcgcc cgggagctgg gcgtttcgat catcgatgga
     5701 ttcacgccag tagccgtgga aggcaataaa gtcacgttta agcatgtacg gttatcgggc
     5761 gaactgacga tggcggcaga taaaattatt ctcgccgtcg gtcagcatgc cagactagat
     5821 gcctttgcgg agttagagcc gcagcgtaac accatcaaaa cacaaaatta ccagacccgc
     5881 gacccgcaag tctttgctgc tggcgatatt gttgagggag acaaaaccgt ggtctatgcc
     5941 gtgaaaaccg ggaaagaagc cgccgaggcg attcatcact atttagaggg agcttgctca
     6001 tgttaacgaa agatctttcg attacttttt gcggcgtgaa gtttcccaac ccgttctgcc
     6061 tctcttcttc gccggtaggc aactgctatg agatgtgtgc caaagcctac gacacaggtt
     6121 ggggcggtgt ggtgtttaaa acgatcggct tttttatcgc caacgaagtc tcgccgcgtt
     6181 ttgatcatct ggtgaaagaa gataccggtt ttatcggctt caaaaatatg gagcagattg
     6241 ccgagcatcc gctggaggag aatctggccg ccctgcgtcg gctgaaagaa gattacccgg
     6301 acaaagtatt gatcgcttcg atcatggggg aaaatgagca gcaatgggag gagctggcgc
     6361 gcctggtgca agaagctggc gcggatatga tcgagtgtaa cttctcctgt ccgcaaatga
     6421 cttctcatgc gatgggtagc gatgtcgggc aaagcccgga gctggtagaa aaatattgtc
     6481 gggcagtgaa acggggttcc acgctgccaa tgctggcgaa gatgacgccg aatatcggtg
     6541 atatgtgcga agtggcgctg gcggcgaagc gcggcggcgc agatggcatt gcggcgatta
     6601 acaccgttaa atccatcacc aatatcgatc ttaatcagaa aatcggtatg ccgatcgtta
     6661 acggaaaatc aagtatttcc ggatattccg gtaaagcggt aaaaccgatc gccctgcgct
     6721 tcattcagca aatgcgcacc catccagaac tgcgcgattt cccaatcagc ggtatcggcg
     6781 gcattgaaac ctgggaggat gcggcggagt ttttattgct cggcgcagca acgttacagg
     6841 tcaccaccgg cattatgcag tacggctatc gcatcgtaga agatatggcg agcgggttat
     6901 cgcattatct cgccgatcag ggatttgatt cgttgcagga gatggtaggt ctggcgaata
     6961 acaatattgt cccggcggaa gatttagacc gcagttatat tgtctatccc cgtatcaatc
     7021 ttgacaaatg tgttggctgt ggacgctgtt atatttcctg ttacgacggc ggtcaccagg
     7081 cgatggaatg gagcgagaaa acccgcacac cgcattgtaa taccgagaaa tgtgtgggtt
     7141 gtctgctttg tggtcacgtc tgcccggtgg gttgtattga gctcggggaa gtgaagttta
     7201 agaaaggcga gaaagaacac ccggtaacgt tgtagaaccc gcgtcacatc cggcatttgg
     7261 ttcctgttgc cggatgcggc gtgaacgcct tatccggcct acaaaaccac acagatcatg
     7321 atgttatgtg cgtttatgac cgaatgcgga ccacattcac atcatttctt acgcgcgtat
     7381 ttcagtgaat ccagcgctac ggcgaagata ataatcgccc ctttgatgat gtactgccag
     7441 tatgggttta cgccgatata cgtcaggcca tagttgatga cggtaaaaat aatcaccccg
     7501 gtcaccacgc caatcaccgt ccccacaccg ccgctgaacg atacaccgcc taccacgcac
     7561 gccgcgatag catccagctc atacataaag ccgaggttgt tggtggcaga gccgatacgt
     7621 ccggcttcta acatcccgcc aaaggcatag aacacgccag acaacgcgta gatcatcagc
     7681 aggttcaggc cgacgttgac accagatact tttgccgctt ccgggttacc gccaatggca
     7741 aaaatgttct taccgaagcg ggttttgttc cacaacaccc agacgaacgc caccgcaatc
     7801 aacgcgtaga aggtgatgta agagagacgg aaactcccca gcgcgacaaa gccctgagca
     7861 aaggtagaga agccactgtc aaaaccagaa attggcgacg ccccgacaaa gtcgtaatag
     7921 agcgagttga tgccatagac gatgatcatc gtgccgagcg tggtaatgaa cggcgtcacg
     7981 ttgagataag cgataatcag accgttgatc aaaccgatca ccgcaccaat ggcacagaca
     8041 atcagaataa ccagcgcaat cggcatcgtc gccatttccg ggaacacttt gttggcgtta
     8101 tccatggact gcaataatgt cgcagccacc actgccgcca gccctacctg acgaccagca
     8161 gaaagatcgg tcccctgggt gacaattaac cctgccacac cgagcgcgat aataatacgc
     8221 accgatgact gggtgagaat attacttaag ttcaacagac ttaaaaatgt tgggtcctgg
     8281 aaaataataa tcgccagcaa aactaaaaga acgacgtaaa taccgccctc tttcaggtaa
     8341 gtaagaaaac ttttcttatt taacgcactc atgggaagcc cctaatctta aaggtgcaaa
     8401 gacgcaagac gcagaatttc gttttgcgtt gttgtttttg tatcgacaat tccggaaacg
     8461 agaccattgc tcatgaccag aatacggtct gttatcccta acaactcagg catttcagag
     8521 gagataataa taatcccctt gcctttcttc gccagttcgg caattaactg ataaatttca
     8581 aacttcgccc cgacatcaat accgcgcgtc ggttcatcga gcattaatat ttctggttgc
     8641 gttagtagcc agcgaccaat aatcaccttt tgctgattac caccggagag cgaaccaatt
     8701 tgcgtccgat gacccggcgt ttttacccgc atcgaatcaa tcacccactg ggtatcgctt
     8761 ttcatccgcg agttatccag taaaccaact ttatttttgt agttgcgaat attggaaatt
     8821 aacgagttaa aaccaatatc cagataggca taaattcccg ttgagcggcg ctcctcagtt
     8881 accagtgcaa atccgtggtt tatggcttcg ttggcattat ggttattgat ctgtttgccg
     8941 tgcaacgtaa tggtgccagc cgatttctcg cgaataccaa ataacgtctc aacaatatcg
     9001 gtacgtttcg cccccaccag cccggcaata ccgaggatct cccctttatg cagatcaaac
     9061 gagacatcgc gaatcgacgg ctggcgcagt gacgttaggt tacgcacctc gaggatgact
     9121 tcgcccggct tgttttcttt gtcaggaaaa cgctgattaa gagaacgccc aaccatcatg
     9181 gcgatgatct tgtccatcgt cagtcctgcc agcggttcgg tggcgatcca ctgaccgtcg
     9241 cgcaatacgg taacttcatc acataactgg aagatttctt ccattttatg cgagatataa
     9301 acaataccgc agccgcgctc ttttaattta cgaataatag tgaacagatg attgacctct
     9361 ttttcggtta acgaagaagt cggttcatcc ataatcacaa ttttcgcgtt ataggaaaac
     9421 gctttggcga tttcgatcat ctgcatttgc gaaacggata atgtgccgac gcgcgcacgc
     9481 ggatcgatat caatatccag ttcatcaaaa attgctttgg tttcgcggta cattttgtcc
     9541 tgatcgacaa acatgccttt ggtgggatat cgccccagcc acatgttatc catcaccgaa
     9601 cgttgtaata ccaggtttaa ctcctggtgt accatcgaaa taccattttc cagtgcctct
     9661 ttggcggaat ggaaatcgat ctctttaccc tggaataaaa tggtgccgga gtctttttga
     9721 taaataccaa acaggcattt taataatgtc gattttcctg caccgttttc ccccattaat
     9781 gcatggatag aatgtggccg gacttttaaa ttaacgttat caagtgcctt aacaccagga
     9841 aaggacttgt tgataccgct catttccaac aagtattccc cggaagacgg agtcgttgag
     9901 ctgaccatat aattttacct tgttggccat acaataaggg cgcagtaaac gactgcgccc
     9961 aatcagtctt atttcttgct gaattcagcc aggttgtctt tatctacgcc aacataaggt
    10021 acgcggacca ctttgttgtc gattttccag ttggtgccat cagccgcacc tttgccatcg
    10081 gccaggtttt tcgccagatc aaaggtcgct ttcgcctggt tgttagcatc gttcagtact
    10141 gtgcccgcca gtgcaccgga tttcaccagc gccagcgctt ctggcagcgc atcgacgcca
    10201 aacaccggaa tgctggactt gttgtgtgct ttcaatgctt ctaccgcgcc cattgccatc
    10261 gcatcgttgt tggcgataac cacttcgatt ttgttggcgt tcgggccaga cagccaggcg
    10321 tccatcttat ctttcgcctg agcggtgtcc cacattgcgg tatctaactg taactgttca
    10381 gttttgatgc ctttatcgtt caattcttta atcacgtaag tggtacgtgc ttctgcatcc
    10441 ggatggcccg gttcaccttt cagcagtacg aactgaatct gaccgtcttt gttcagatcc
    10501 caaccctgat tcgccgccca gtgtttagca atcaaatcgc cctgaataat gccggactct
    10561 ttggagtcag tgccaacgta gtaggctttg tcgtagctat ccagcgcctt acgagacggt
    10621 tctttgttga agaaaaccac cggcacgttt tgcccacgcg ctttctcaat caccgtaccc
    10681 gcagctgccg ggtcaaccag gttgattgcc agtgccttca cccctttcgc cagcaatacg
    10741 tcgatctgat cgttctgctt ggactggtca ttctgagaat cattcatcag cagctgaaca
    10801 tctggcgcgg ctttcgcatc ttgctcaata gccttgcgca ctacagacat aaagttatcg
    10861 tcgtacttat agattgttac accaatgcga gtatcagcag cgtgtgcagc ggcaccgaat
    10921 aacatgctgg ccatcacagc agacagggtt aacaccttct tattcatggt atctccggtt
    10981 tttcttatgc agggtagtgc ttgagataaa tgctcggcgg ggcagtagag ttaatgaagt
    11041 gttactgaac gccgaagctc acttttttaa attcgttctt ccatgcccgg taacgctcca
    11101 gaaaacggct ttaattgttg ttatgacgct attacctcgg tgaaagtgat tattcactgt
    11161 tacatacggg ttacaacgtt aaaacggtgc aatcatagct atcacattgt taagatactg
    11221 tgaaatcact cacagattga aagcggttac atcgcctgat ttgttgagtt agtgatcgcc
    11281 gccgcattct ggcgcgttgc gacagaatgg cgacgcacta acgtcggcat aaaacagtgg
    11341 ctggcacgag gatcaatatt gcctgctgcc ccctgcaagg ccagttcggt ggctaattta
    11401 gccattgaag caatgggata acgcacggtc gttaattgcg ggtcggtgta acgggcgatg
    11461 ggaatatcat cgaaaccgat gattgagaga tgtaacggaa tcgcaatgcc attatctttt
    11521 aatgctgtca gtgcgccagc ggccatattg tcgttataag caaatatagc ggtaagttgt
    11581 agattgcgcc ccagcagttc aaccatcgcc gcctcaccgc ccggcatgtc cggcgtacca
    11641 gtgccaatcc agctttccgg cggaataata tcctgctctt tcaacgcact catccagcct
    11701 gctttacgca tggcgtcatc ttcaatgccg tgactggaag aaagataacc aatacgttga
    11761 tgaccgttat tcagcaacat gcgcgtcgcc attcgggcac cgctgagatt atccaggcaa
    11821 acgcaacgat gggcgtaccc cggcacaacg cggttgatta acaccatacc gggaatgtta
    11881 tccataaatt gcgccagttc atcgtcactc aatgcttttg agtgaacaat caacgcatta
    11941 caacgctggc gaattaacac ctcaatggcg tgacgctctt tttccgcttc atgatagcta
    12001 ttgccgatta gcacgtattt ctgatgctgc tgagcgacta gatccaccgc ttttaccagc
    12061 gcgccgaaaa acgcatcaga aacgtccatc accaccacgc caatggtgtc gctaacctga
    12121 gttgccagcg cctgggcatt ggcgtttggc cgataatcca gctcactcac ggctttcatt
    12181 actgcttcac gcgtgtcggc actgaccagc gtgctgttat tgagcacccg ggaaaccgtt
    12241 gccacagaga cgccagccta acgcgctaca tcacgaatgg tgatcatatt cactacctgt
    12301 tatgagattg cagtaaatga ctgcttgctg gcggctattt tgtcagcact gaatacagga
    12361 cttcgtgaat cgagtcacag caatggaaac ggttacagcc gttttgttaa cgaatgtgat
    12421 ccagatcgtt atcttgatgt tttagatatt gccgttccgg cagcacgcaa agttaactga
    12481 cgccagagcc attccaccgg cccctggcgg aagtaacgca gccagataac agagaagagg
    12541 atattcgcca gccataccgg aataacaaac gccagcagct ccaggcggtc aaaatgcata
    12601 aacaaaccga ggtggtaaaa aagcatggta caaatcagcg tttgcaatag atagttggtc
    12661 agcgccatcc gtccgacgca ggcgatcgca agcaccagct taaagcggct caattgcggc
    12721 cagaagccat aaaacagcga cgcatagcca atcgcctgaa acggcgcact cagttcccgc
    12781 ggcatttgaa gtaagaaggc acaccagcga tacgcccagt ccagctgcca ttgcagggcg
    12841 atggcaggaa ggttaatggt caccccaatc gccaccagca caaaaccagt acgacgatag
    12901 tgacgtaagc tgaactgccc tttcagccag ccgctgcgca tcagcgccgc accaatgagc
    12961 atcatccctg ccagttgcca gccatactgt gcgcccagtg ccagtaaact gttgccaaca
    13021 ccatcggcac gattactgat cgcttcaacg ccgccgtgaa gcttccagta tttttcatac
    13081 aaaatagccg atgcatccgg cgtccaggtg cggctggtct ggctgtcgga aatcagcccc
    13141 aataacagca aaacgccaag ccccaccaga taaagcatga cgcctgtatt aaacaggctt
    13201 tttaccgatg gcgcatcgcg caccagccgc cagcagatta agcccaccag cccgtaagcc
    13261 agcagaatat cgccgtccca gaacaataaa ccgtgaataa aacccagcaa gactaacagc
    13321 gttaaccgcg actggatcca gcgtctgcca cggggcagca acatttgcag gcccgcacca
    13381 aacagcagcg caaaaagcgt gaggaatttc acctggccga tgagatcgag aaatgcccag
    13441 gtccatgcat cctgcggcgt aatagcgccg taccaggcgg gattgagata agccgccttt
    13501 ggtagcccaa aggcgctgat gtttaatagc aggatcccca gaatggcgac gccgcgaaca
    13561 aaatcgagcg ttacgttgcg ctccatggtt cctgcctttt aatcagttgt gatgacgcac
    13621 agcgcgcaga aactcgtggc gcgtattctg actggatttg aacaatccac caagagaggt
    13681 cgttgttgtg gcactggttg catcgcggat gccacgcgct ttcacgcagt aatgcaccgc
    13741 atcaatggag acagccacgt tattggtgcc cagcagcgtt tgtagcgcaa taagaatttg
    13801 ctgcgtcaga cgttcctgca cctgcggacg ctgggcaaag aactgcacga tacggttaat
    13861 ttttgacaga ccgatcaccg aatctttcgg gatataagcc accgtcgctt tgccatcgat
    13921 ggtaacaaaa tggtgttcac aggtactggt cagagtgata tcgcgcacgg tgaccatttc
    13981 atcgaccttc attttgtttt caatgagggt gattttcggg aagttggcgt aatccagacc
    14041 ggagaaaatt tcatcgacat acattttagc gatgcgatgc ggcgtttcca tcaaactgtc
    14101 atcagccagg tcgagattca gcagctgcat gatttcggtc atatgaccag caataaggct
    14161 tttgcgcgtt tcgttatcca tttcatgcac gggcgggcgc agcggtgttt ccagtcctcg
    14221 cgcaactaac gcttcatgaa ccagggccgc ttctttactg agtgatggca tttatgattt
    14281 ctcctgcagg tgtgacgcct ccgccctgcg tgggggcaaa gttattaagc tgatttacag
    14341 cctgattatt gtgcgtgagg cggcgcacat aatccagtat tcacagcgat aattattgta
    14401 attgccgctg actttcatca gcagatgtta aaacatcgtt atgcaaatac ggaagtgaaa
    14461 gttactcaca gcacattgaa taaacggtat gatgaagaaa ttgcaaacaa cacaacaagg
    14521 agccacgcat ggaaatgctc gaagagcacc gctgttttga aggctggcag caacgctggc
    14581 gacacgactc cagtacctta aactgcccga tgacgttcag tatctttctc ccaccccctc
    14641 gtgatcacac cccgccacca gtgctgtact ggctttccgg attaacctgc aatgacgaga
    14701 acttcaccac caaggcgggt gcccagcgcg tggcggcaga actggggatt gtactggtga
    14761 tgccagacac cagcccgcgc ggcgaaaagg ttgccaacga cgatggctac gatttaggcc
    14821 agggcgcagg cttttatctt aatgccacgc aaccgccgtg ggcgacgcat taccggatgt
    14881 atgattatct gcgcgatgaa ttaccggcgc tggttcagtc gcaatttaat gtcagcgacc
    14941 gctgcgccat tagcggtcac tcaatgggtg gtcacggtgc gctgattatg gcgctgaaaa
    15001 atccgggtaa atacaccagc gtttcggcct ttgcgccaat tgtgaatccg tgcagcgtcc
    15061 cgtggggaat caaagcgttt agcacctatt taggtgagga caaaaatgca tggctggaat
    15121 gggatagttg cgcgctgatg tatgccagta acgcgcagga tgcgatcccg acgcttatcg
    15181 atcagggcga taatgatcag tttcttgccg accagttgca acctgcggta ttggcagaag
    15241 ccgcgcgcca gaaagcgtgg ccgatgacgc tgcgtattca gccgggatat gatcacagtt
    15301 actacttcat cgcctctttt atagaggatc acctgcgctt ccatgcgcag tatttactga
    15361 agtgaaagtc cgcccggttc gccgggcatc ttctcatcag aagcgataat ccactgccat
    15421 aaagtaacga cgtccgtctt cgttatagct gtagtcgtca cgactgagat ccttgtcgcc
    15481 aaggttcagc acgcctgcgc gcagtttgac gtctttagtc acctgccagg ccgcgccggt
    15541 attccagatg gtataaccgc ccggtgtttt agccgtcgcg ctgtcggcgc gtttctgccc
    15601 ggtatagtga ccagaaacat agaatgacca gtcttccagc gccagcggtt tccagtccag
    15661 cgtaccgtta gcagtatgga acggcagatc ggatagcggt ttgttttcgc cgttgctgac
    15721 atcacgacca tcgttgtagg tgtagttgat cgacagtttc cattcatcgt tgaacggaat
    15781 tttcagttcg gtttccacgc cctgaatacg agctttgtta acgttgtagt agctaaatac
    15841 cggtatgcgc cgtccgttag cgcccgtctc aaaaccaaca aagttttggt agcccggtgc
    15901 agcgttgacg tcagacgtac ggctgatgct gatacgatct ttcacatcgt tacggaaaac
    15961 ggtaacgctg gattcaaccc cttccagcca gccttcttca cccatgtagt aaagccccag
    16021 ctcccaactt tcgctggttt ctggtttcag atccgggcta cccacaatct tacatgcgcc
    16081 acggcaggaa ttgctcgtcc agtcagggct aagttgcaac agagaaggtg ctttaaatgc
    16141 cgtcgcccag ccccctttca ccgttacggt gtcggtggcg ttataaacca ggtaggcacg
    16201 cggactccag tgttcaccgt aggtttcgtg atcgtccata cgcacgccgg tcgtcagcgc
    16261 cagcggctcg aagatccgcc attcatcttc cacaaacagc gcgtactggc tggcagacgt
    16321 tttggagctg gttcccccgg tcaggttcac cgcatcgcta agtttgtcgt gacgccattc
    16381 accgccaacc gtgagaaact gattaatcgc cgtcagcggc aacgtgtatt tgccgtcgac
    16441 cgtattgctt tcggaagtta tcgggctgct gttgccaggg tttttgttct cgactttctc
    16501 accgtagtat ttcagttcgc tggtgccgta atcccaacgc ccattatggc tgacggagta
    16561 gttctggcgt tccaggcggt ttttgtccag cgagtcggaa tcacgatcct gacggtcgaa
    16621 accgtatccg gcagtaaaat cgtgattttg attcggtgtc caggcaaatt cgacattgcc
    16681 gtcgcggctg gagaatcctt caatacgcgg cgtttctccg gtatcggtgg tcgttgagtt
    16741 ttgcgggtca tccttttcac gttttgccag gctgccgtaa gctttcattc ccagcacacc
    16801 atcaattaat ggtccactgg taaagaactg accgttatag gtatcaccgc gatcgcgatg
    16861 ttcctgaatg gtggtatcga cggtaacggt acccgaccat ttctgaccga tttttttggt
    16921 gatgatattc actacaccgc cgagcgcatc ggaaccgtac agcgacgaca tcgggccacg
    16981 gaccacttca atacgttcga tggaatcgac cgggatccag ttcagatcga aatcattgtg
    17041 gcggaagacg gcattgcggg agttcacgcg tttaccgtcg acgagaatca gggtatagct
    17101 gctgtccaga ccacgaatac taacgccctt acggttatcc ccttcgttcg tcagttgtac
    17161 gccaggcact tctttgagga catccttcag attctgtacc ggttttcgct gcagatcttc
    17221 ctgggtaatg acgctgatac tggcgggtgc atctttaagg ttttgttcaa cggaagatgc
    17281 agtgacaacc atcgtttcgc catcgtcatc gaccgctaac acaggccata cacaagaaat
    17341 agcggacaaa cacagcccga cccgtacgaa agggttcaac ctaaacattc catatctcca
    17401 tgaggtaact acgaaaataa aatgggtatc gctcacatct tctttacgtc cccttgcgta
    17461 cggcagcatc gcggtggact tatttgtatg caggtgatca tcccagaaag ccggaccagc
    17521 ctctgatttg tcagcctttt ttgattgtag cgtaacgata atgcaaacga taacaattat
    17581 caatccaaat gttaaatttt atatccgcat gatttgtcag ggaaatggaa ttgaaaaagc
    17641 cctctcggtt gagagggctt agcaaggaag ggaggaaact tatttcttat cgttctgcgg
    17701 gaacttcatt tcgctgtagc gtacgaagtg agttccttta atcagcttgt agccgaacca
    17761 aataatcagg aacagcggga taccaatata cgttgccgct acgccgcccc agtcaatggt
    17821 atctttcagg aacgcttcgt agttctggcc caaagtgata atcagacaca gaatgaatgc
    17881 gaagatcggc cccagtggga agaaacctga acggtacggc agatcgttaa tgtcgtgtcc
    17941 ctgcaatacg taaccgcgac ggaagcgata gtggctaatg gcaatcccca gccaggcgat
    18001 aaaacccgtc atcccggagg tgttcagcag ccacaggtat accgtctggt tgccaaacat
    18061 ggaggtcagg aagcacaaac cggcaatcac cgtcgtcgca tacagcgcat tacgcggcac
    18121 gccaccacgc gacaatttag cgaaaatgcg cggcgcttta ccgtcacacg ccagggtgta
    18181 cagcatacga gtagacgcat acataccgga gttacctgct gacagcaccg ccgtcagaat
    18241 aactgcgttc atcaccgccg ccgcagagag cagacccgcg tgctggaaca ccaaggtgaa
    18301 cggactgacg ctgatgtctt taacatcgtt acgcagcagg ctcggatcgg tatacggaat
    18361 aatcaggctg ataatcagga tcgcgaacac atagaacaac aggattcgcc agaatacctg
    18421 acgtaccgcg cgtggaatgt ttttcgccgg atcttcggac tcgcctgcag caataccgat
    18481 cagctcggtt ccctggaaag agaagccgac aatcatagct acgccgatca tcgccgcaaa
    18541 accaccagca aacggcgctt cgccgattgt ccagttgctc cagcccgcag gctgcgcgcc
    18601 tttgaagata ccgataatca tcagcacgcc aacgatgata aagacaataa ctgtcgtgac
    18661 tttgatcagt gaaaaccagt attccgcttc accaaagcca cgaactgaga tgtagttcag
    18721 caggaagata acgccgagga acaacgcact ccagatccag cccggtgtat ccgggaacca
    18781 ccagctcatg accagctgtg ctgcaaccaa gtcaacggca atagtcaccg cccagttgta
    18841 ccagtagttc cagcccagcg cgaagccaaa gccttcttca acatagttct gaccgtaagt
    18901 ggcaaacgaa ccggaaaccg gcatatatgc agccaattca ccgagactgg tcatcaggaa
    18961 gtaaaccatc aggccaatca gcatatacga gagcaatgcc ccgcccggac ctgcctgaga
    19021 aatcgttgcg ccagaggcaa caaaaagacc tgtaccgatg gaaccgccaa tggcaatcat
    19081 cgtcaggtga cgcgccttta attcacggcg taagcccggc gcttctgtag ttttagtttc
    19141 ggaacccata caaaaatgct atccatctta aaaatgaggc gcgattgtag cagacgattg
    19201 gcaatccttc cggcagaaat acacggttat aagacacctt catgatcgcc cagggattat
    19261 aagtaaagca gccaatctct tttctggcga atgcatgctg agggcgctgc gtcacgaaat
    19321 cgacacagca ccagcatgtt cttgtacagc accttaacgc ggtacattcg cgggatcgca
    19381 atagtccaga aagcgccgta gcgcgttgga aaggtgtttt tgccgatgat gtatacgcca
    19441 caacgtacgc atcaggcgcg ggagagggac cgcaacttca cttaatgtgc ctgcctgcaa
    19501 ttgatcttca atcacacgtc gcgacaggca actaattccc aacccatgac gcaccgcatg
    19561 tttgattgcc tcggagttac ctaattccat cgccatctca aacttcggta aatgtgacag
    19621 caacagataa tcgacaatct cccgcgtgcc ggaaccgcgt tcacgcagga tccacggcgc
    19681 agcggccagc tgttctaagg tgaccggacc acgagccaac ggcgaagtcg gcgcggcgaa
    19741 aaccaccagc tcgtcttcca gccacggttc agaaatgatt tcagtgctgt ggcacggtcc
    19801 ttcaataaag ccaatatcaa cgcggaaatc cagcaccgct tgcatcacgt cctggctgtt
    19861 cccaacgcta agttcaatcg gcaactgcgg ataatcatgg cgataacggg cgataactgc
    19921 aggcagaatg tagttaccga tggtactact ggcatagata cgaatcgcgc cgttgtcttc
    19981 gcgaaacagt tgttcgattt caaccgcctg ttcaagcaat gccaacgcac gcggatagag
    20041 cagccgcccg tgttcattaa caaccagtct tttccccacg cgatcaaaca gttgcacgcc
    20101 aagctgccct tccaggtcgg tcaaggctgc gctcactgct gattgcgaca acgccagcat
    20161 caccgacgcc tgggtggttg atccactttt caatacttct gcaaaaactt ccaactgccg
    20221 gagggtgatg tgcatagtcg cttaccactt ataaagatta attataaata tataatcaat
    20281 tttattttta aaccagttag tcgtaacctt atgcccggtt aaaggagagg gttatgacga
    20341 atatcacctt acagaaacaa catcgtacac tgtggcattt tattccgggg ttagccctga
    20401 gtgcagttat caccggggtc gccctgtggg gtggttccat tcccgcggtt gcgggtgccg
    20461 ggtttagtgc cctcaccctc gcaatcttgt tggggatggt tttaggcaac accatctatc
    20521 cgcacatctg gaaaagctgt gacggtggtg tgctgtttgc caagcaatat ttattacgtc
    20581 tgggtatcat tctttatggc ttccgtctga cgttctcgca aattgccgat gtcggtatca
    20641 gtgggatcat cattgacgtg ttgacgctgt ccagtacctt cctgctggct tgcttcctgg
    20701 ggcagaaagt gtttggtctg gataagcaca ccagctggtt gatcggtgcc ggtagcagta
    20761 tctgtggtgc tgccgcggta ctggcgactg agtcggtagt gaaagcggaa gccagtaaag
    20821 taaccgtggc tgttgcaacc gttgttatct tcgggaccgt cgcgattttc ctctacccgg
    20881 cgatatatcc gctgatgtct caatggttta gtccggaaac tttcggtatc tacattggtt
    20941 ctactgtgca cgaagtggcg caggtggtgg cggcaggtca tgccatcagc ccggatgcgg
    21001 aaaacgcagc agttatttcc aaaatgctgc gcgtgatgat gctggctccg ttcctcatcc
    21061 tgctggcggc gcgtgttaaa cagctgtctg gggcgaacag cggcgagaaa agcaaaatca
    21121 ctattccgtg gtttgccatc ttgttcatcg tcgttgccat ctttaactcg ttccacctgt
    21181 taccgcagag cgtggtgaac atgctggtaa cgctggatac cttcctgctg gcaatggcga
    21241 tggcggcgct gggtctgacc actcacgtca gcgcgctgaa aaaagctggg gcgaaacctc
    21301 tgctgatggc actggtactg tttgcctggc tgattgttgg tggtggtgcg attaactatg
    21361 tgattcaaag cgtcatcgca taaaccacta catcttgctc ctgttaaccc gctatcatta
    21421 ccgttttcct ccagcgggtt taacaggagt cctcgcatga aatacattgg agcgcacgtt
    21481 agtgctgctg gcggtctggc aaatgccgca attcgcgccg tcgaaatcga cgcaaccgcg
    21541 tttgccttgt tcaccaaaaa ccaacgtcag tggcgtgccg caccgctcac gacgcaaacc
    21601 atcgatgaat tcaaagccgc ctgtgaaaaa tatcactaca catcggcgca aattcttccc
    21661 cacgacagtt atctgattaa cctcggacat ccggtcactg aagctctgga aaaatcgcgc
    21721 gatgccttta tagatgaaat gcagcgttgc gaacaactgg ggctttcttt gctcaacttc
    21781 caccctggca gccatctgat gcagatttca gaagaggatt gccttgcgcg tattgccgaa
    21841 tccatcaaca ttgcgctgga taaaactcaa ggtgtaacag cggtgattga aaacaccgcc
    21901 ggtcagggca gtaacttagg gtttaaattc gaacatctcg cggcgattat cgacggcgtg
    21961 gaagataaat tccgcgtcgg cgtctgcatt gatacctgcc atgctttcgc tgccgggtat
    22021 gatttgcgta ctccagccga atgcgaaaaa acgttcgctg attttgcccg tattgtcggc
    22081 tttaagtatc tgcgcgggat gcaccttaac gatgcgaaaa gtacctttgg cagccgcgtt
    22141 gaccgccatc atagcctcgg tgaaggcaat atcggtcatg atgcgttccg ctggatcatg
    22201 caagacgacc gtttcgacgg cattccgctg atccttgaaa ccatcaaccc ggatatctgg
    22261 gcagaagaga tcgcctggct gaaagcgcaa caaactgaaa aagcggtggc ctgatcgatg
    22321 aacgacaggg aaaagcagat tcttaaaatt ttacggcgta acccgctgat tcaacagaat
    22381 gaaatcgccg atattctgca aatcagccgc tcacgtgttg ccgctcatat tatggatttg
    22441 atgcgcaagg gtttgattaa aggcaaagga tatatcctta ccgagcagga ttactgtgtg
    22501 gttgtgggtg caatcaatat ggatattcgc ggaatggcgg atatccgtta cccgcaagca
    22561 gcctctcatc caggttctgt tcactgttcc gcaggcggcg tcggacgtaa tatcgcgcat
    22621 aacctggctc tgcttggtcg ggatgtgcat ttaatatcag ctattggcaa cgatttttac
    22681 ggcgagacat tactggaaga aacgcgccgc gcgggcgtga acgtttcaaa ctgcattcgt
    22741 ctgcatggac acagcactgc aacttatctc gctatcgcta ataagcagga agaaactatc
    22801 ctggcaatca atgacacaca tattctgcaa cagctaacgc ctcaactgct caatacttca
    22861 cgggacttga ttcgccatgc tggtgtggtg ctggctgatt gtaatctcac acctgaagcc
    22921 ctggagtggg tctttaccat tgctgatgaa attccgatgt ttgtcgatac cgtttccgaa
    22981 tttaaagcga acaaagtaaa aaactggtac agccggatcc acactctgaa accaacacaa
    23041 aatgaactgg aaattttgtg ggggcaaccg attaaagacg acaacgatcg catcagagct
    23101 gtaaattcgc tgcatcaaca aggtgttaaa cgcatttttg tttatctgaa agatgaatct
    23161 gtattttgca gcgataagga tggagaacaa tttttgctga ccgcgccagc gcatacgaca
    23221 gtagacagtt ttggtgctga cgatggtttt atggcgggcc tggtatatag ctttcttgaa
    23281 ggatacagtt tccgcgacag cgcccgtttt gcggtagcct gcgcggcaat ttcgcgcgcc
    23341 agcggcagct taaacaaccc taccctgtct gccgataacg cgctttcatt agtgccaatg
    23401 gtgtaacaat gttgccggat tcgacgcttg accgcatctt atccggctca taaattgcgc
    23461 actacgctaa accaataaag aatcctgcaa tagtagcact catcaggtta gaaagtgtcg
    23521 ccgccgccag cgcgcgtaaa ccaagttggg cgatttccgg cgcacggtgt ggcgcaaccg
    23581 cagaaaacgc ccccaccacc accccgatag aaccaaagtt agcgaaaccg cacaacgcga
    23641 aagaaataat cgccacggtt ttagcatcca gagtgccacc cgtttgcaga tatggcgaga
    23701 aattgagata agcgacaaat tcattgatcg ccagcttctg cccaatcaaa ctcccggcaa
    23761 gatttgcatc actccagtca acccccatca cccacgccaa tggggccaac aggtaaccta
    23821 aaatcgactc cagcgaagca tgttcaaaac caaaccagcc gccaacaccg ccgataatac
    23881 cgttaatcaa cgcaattatt gcaacaaatg ccataacaac tgtcgctaca ccggcggcga
    23941 ttttcagccc ggtcattgcc cctgtcgcag cggcttcaat aatgcttttt ggcggtgttt
    24001 cggtgaaaga gaggttatta aaagaaacct gcgaagattc cgtagccggg cttaacaggc
    24061 gggcaaacaa gatcccgcca gggatcgcca ttaacgatgc cgccagcaaa tattccacag
    24121 gtacgcccag tgcggcgtaa ccaatcattg tcgaaccagc aatcgaggcc atgccactac
    24181 aaatcgctgt aaataattca ttgcgattca gacgatcgat aaagggtttg acgatcgccg
    24241 gaatttcgtt ttgcccgagg aaaatggtgg tgaccgcgac gaatgactcg atcttgctga
    24301 tatttaaagc tttctggaag ataccgccga gaatgcgaat taagatcccc atcacaccga
    24361 tgtagtagag aatactcacc agtgcagtga cgaagataat tgccggtaat accctgaaac
    24421 caaagataaa tcctgcgcca tcaaataagg tatccatttt cggtccgacc agagaaccga
    24481 agataaatgc gctacctgcg tcgctgtacg ccatcacttt gtggacgcca aaagcgactt
    24541 tttctgcgac ccaacgccct ggcggtaacc aaagcataat cccgccaatc acgacctgta
    24601 acactaacgc cgcgccaacg gtacgcaggc tgatcttctt cttgtttact gacagtaaaa
    24661 acgcaattgc cagcagtacc accattccca gaacacttct catgacatcc atagtgatta
    24721 tctcttcatg tcattttggg cgacggcttt ctgcccatca gaaatagccg ggcctgcatc
    24781 ttgcgatact gtcccggctt gttatcaaca cttaatgggt ttttatgtag ccgcgtacgc
    24841 actcttcgac taatccccag aaccagtcgg tatcaatagt gatgccgact ttagtattgg
    24901 cgggcttgcc aagaacgccc agctcgtcgc agacggtacg cccatagcaa gggccactgt
    24961 tgacgtccac ttcgacgtac atctcctggg ttttaatgcc atcagggtta atcagataac
    25021 cgatgcaggt ggcgtcgtgc accgggccgc cagccaggcc gtagttttcg aactgcgttt
    25081 tgagagtgaa gttcatgatg tcgctgaaca tctctccggc agggccgcct gccctttcca
    25141 ttcgtgcaat tacgtccggg gtgcaaacgg tctggttggt aagatcgagg cccatcatca
    25201 ctaatggaac gccggaggtg aacaccacgc gtgcggcttc cgggtcggca aagatattga
    25261 actcggcaga tggggtgaag ttgcctgtac cgtaagcgcc gcccattagc acaatttcac
    25321 ggattttggg caggattgcg ggttgcatac gcattgccac cgcgatattt gaaagcggac
    25381 caaccggcac cagagtgata tcgccatcgc ttgccataag ggtatcaatg atgtatttca
    25441 ccgcgtgggt actttctgcc tggcgggtca gcggctcgaa taccgggcca tccagtccgg
    25501 tttcaccgtg aatattatcg gcaacgattt gttgacgcat aatgggttgc ggcatccccg
    25561 cataaaccgg aacattaatc tccagtttct ggcaaacatt caggccatta attaatgttt
    25621 tatcaagcgt ctgattaccc gcaacaatgg tgatacctaa taaatcaatc gccggatgtt
    25681 tcgccgccat cattatagca atagcatcat catgacccgg atcacaatcc agaataattt
    25741 ttctcttttc cattgtttat ttcctctgtt tccagttgcg ttattttttc tacagcaacg
    25801 aaatttcgat cgggccgatg attgaatctt aacaacagcg tacgaatgct aaatatgaga
    25861 aatctcatag cggataaaca tcgtgaaaga aatccacaat aatgatctta agcagcaatt
    25921 gatgagtgaa tccgcgttta aggattgctt tttaacggat gtttcagccg atacgcggct
    25981 gtttcatttt ttagcgcgtg actacattgt gcaggaaggg caacagccgt cctggctgtt
    26041 ttacctgacg cgaggccgcg ccaggcttta cgccacgcta gctaatggtc gcgtgtcgct
    26101 gctcgatttc tttgccgccc cctgttttat tggcgagatt gagttaatcg acaaagacca
    26161 tgaaccgcgt gcggtgcagg ctattgaaga gtgttggtgc cttgcgctcc ctatgaaaca
    26221 ttaccgcccg ctgttattaa acgacacgct atttttacga aaactctgcg tcaccttaag
    26281 tcataaaaat tatcgtaata ttgtttcttt aactcagaat caatcatttc cgttagtgaa
    26341 tcgcctggca gcatttatat tactctcgca ggaaggtgat ctttatcacg aaaagcatac
    26401 gcaagcggca gagtatttag gcgtttctta tcgacatctt ttatatgttc tcgcgcaatt
    26461 cattcacgac ggtttattaa ttaaaagcaa gaaagggtat ctaattaaaa acagaaagca
    26521 gttgtcagga ctggcgctgg agatggaccc ggagaataaa ttctccggga tgatgcagta
    26581 aaaattattg gcaatagcac gattgccgga tgcaacgctt aataaggcgt acatccggca
    26641 atgaaactca agctaaacca ataaagaacc cggcaatggt cgcgctcatc aggttggaaa
    26701 gcgtcgccgc cgccagcgcc cgtaaaccaa gctgggcgat ttccggcgcg cgttttggcg
    26761 aaatagccga aaacgctcca acgacaacac cgatagaacc aaagtttgca aacccacaga
    26821 gggcgaacga gataatggca atggtcttga catccagcgt gccgctcgtt tgcagatagg
    26881 gcgagaaatt gagataagca acaaactcat taatcgccag tttctgccca atcaaacttc
    26941 cggcaagatt tgcatcactc cagtccaccc ccataatcca tgccaacggg gccagcacat
    27001 agccgaaaat gctttccaga gagacattgg caaaaccaaa ccagccgcca actccaccaa
    27061 taatgccgtt gatcagcgca atgatcgcta caaatgccat taccaccgtc gccacaccgg
    27121 cggcgatttt cagcccggtc attgcaccgt tcgccgcagc ttcaataatg ctttttggcg
    27181 gtgtttcgct aaacgacaga ttttcaaacg taacttgcga aggttccgtc gccggactaa
    27241 gaatacgtgc aaacaggata ccgccaggaa tagccatcag cgaagcggct aacaaatagt
    27301 caatcggcac ccccattccg gcatagccaa tcatcatcga acccgcaata gacgccatgc
    27361 cgctacaaat agccgtaaat agctcgttgc gattcatgcg atcaataaaa ggtttaacga
    27421 tcgccgggat ctcattttgc ccgaggaaaa tagtggtgac tgcgacaaaa gattcgattt
    27481 tgctgatgtt gagggctttc tgaaaaatac tgccaagaat acgaatcagc agccccatta
    27541 cgccaatgta gtacagcaag ctgataagcg cggtaacgaa aataatcgcc gggagtacgc
    27601 gaaaggcgaa gataaagccc gcaccgtcaa acagaacatc cattttggga ccgaccagcg
    27661 aaccaaaaat aaaggcactt cctgcgtcac tgtaggacat cactttatgg acgcctaatg
    27721 ccgcctgctc tactgcccat tttcccgggg gaaaatagag catgatgcca ccaatggcaa
    27781 tttgcagcag cagcgcagcg ccaaccgtgc gcaagctgat gctcttttta tttaccgaca
    27841 acacaaaggc aattgccagc aataccacca tccccgcaac acttctcatt atatccataa
    27901 tgattttccc ttcatgccag taaacccggc gtcagcgcca ggttttggta tgtttgatga
    27961 gtacgggcga cggctttctg cccgccagaa attaacccac gagacgctga tattctttgg
    28021 caatttcgct cgccagaatg gcgttattaa agaccagttg aatattggat ttcaggctat
    28081 caccgccggt tagctccgca acacgcgcca gcaggaacgg tgtactctct ttcccaataa
    28141 caccctgttc ttcggcttca gcaacggctt gatcaattgc tgcattgatg gtttcttctg
    28201 gcatcgcaaa ctgttccggg atcgggttcg ctaccaccag gccgccgttc aaaccggttt
    28261 gccacttcac tgccatcgcg cgagcaattt cgcgggcact gtcaagacga atgctgactt
    28321 caaatgggct ggttcggcag aaaaacgcag gcagcgcttt tgtctgataa ccaattaacg
    28381 gtacaccgaa ggtttctaaa tattctgttg ttaatcctaa atcgagaata gattttgccc
    28441 ccgcacaaac aacagtgaca ttggtatttg ccagttcctg taaatcggca gaaatatcga
    28501 aggtatgttc cgctccgcga tgcacaccac caatacctcc ggttgcaaaa actttaatcc
    28561 cagccagtgc tgcaataatc atggttgatg caacggtagt cgcgccattt ttccctgcag
    28621 caacgacaaa aggtaaatca cgacgactaa ctttcgtcac gttatgtcct tcacggccca
    28681 gcaattcgat ttcttcttta cttaagccca ctttcatcac accgccaata atggcgatag
    28741 tggctggtac tgcgccctgt ttacgaattg tttcttctac ttcaattgcg gtttgggcat
    28801 tttgtgggaa tggcattccg tgagaaatta tggtcgattc cagcgcaaca accggttttt
    28861 tattttttaa agcatcctgt acttcagcgg atatttgtaa taattcaggg gacagagtta
    28921 attcagacat tctgtgttct ccactaacga tctaacattg gcaattgata attcagggtt
    28981 gttggtatat tcacaggcaa gtgccattga cgaacatccc tgcgcaaatc gaacagaatc
    29041 gataaacggc ataccgtcta cccaacagga ggccagcccc gccatcatgg cgtcgcctgc
    29101 cccagtaaca ttgatgacat tcgttttaat aggggccgac cacccacttt caccgttgat
    29161 atcactgtag taaacgccat cccctcccat gctcagcacc aagcggttca ggccgtgttg
    29221 atgaaaccag gcggcgacct ttgcgacatc ttcacgtccg gaaagtgcaa ttccgctcaa
    29281 cgtttcggct tcaagacggt taggttttaa cgtatggatt ttgctcagat gatcgcggat
    29341 tttgacgcat ttccatgccg ataccggatc aataaataca ggcgtttccc cgctattttc
    29401 cagaacccag accagcacct cttcactaag gttgcaatcc gcgacaataa cctttgcacc
    29461 gcgaataaac tcctgatgct gggcaagaaa ttctgcagaa atacagtcac taatactcat
    29521 atcatttatc gcgacaagca tttctcctgt attatcgagc agtgataaat agctggatgt
    29581 attctcaccc ggaacaatta agcatttatc gacataaaca ccggattgat tagtttgcgc
    29641 cagtaatgat tgcccataaa aatcaccacc tactgcactt aaaagccagg catttttccc
    29701 cagtaacgcc agattatgag cgatattcct tcccacgcca cctggggtaa atttaatttt
    29761 tccgggattc gaatccgcat aattcaatgg atgatgtgaa tacccagcca catcaatatt
    29821 cgccgaacca ataattacta tgtagtcctt ttcgcgcata gcacgccccc tggcaataaa
    29881 ttgccaacga agaaattggg taaaatagcc attacagtca ataagactat tagagcacgt
    29941 gttcagattt aaacacatgt tcataataag catatttaac gtaatgtaaa tcgatcagaa
    30001 aaaacgaaat cggccagata tgaaggcggt cacaaagttt gtctatttat gcagcaacaa
    30061 aagagaaagt tgtgatgtgt aacacacaat atggcaggca agaaaaaggg cagagatgtt
    30121 tctgccctcc agattgttga caacgcgcgc tttgttcatc ctggatgtgg cgtaaacgcc
    30181 ttatccgacc tacaaaagcg caaaaaattc aatatattgc agggttcatg taggtctgat
    30241 aagcatagcg catcaggcaa ttttgcgttt gtcatcatcg gggcagagat gtctcatccc
    30301 cgataatgaa gataaattat gcgttttttt ctactatttg tgtttccggg cgtttcagga
    30361 aggcataggc caaacccgcc accagcgtac cggcaataat tgctaccagg taacccaata
    30421 ccggcgtaat agcgccaggg atcagcagaa caaacagacc accgtgtggt gccatcagtt
    30481 tcgcaccaat cgccattgag attgcgccag tcagcgcccc accaacgata cagcacggta
    30541 gcacacgcat cggatcacga gcagcaaacg gaattgcacc ttccgaaata aagcacagac
    30601 caagtaccag agcggctttg ccaccttcct gctgcgcttt gtcgaatttg cgacgcgcca
    30661 ccattgttgc cagacccatt gccagcggtg gcaccatacc tgccgccata atcgccgcca
    30721 tcgggccata ggtttgagta ctcagcagac ccacaccgaa tgcgtacgct gctttgttta
    30781 ccggaccgcc catgtcagta cacatcatgc caccgaggat cgcccccagc agaaccgcat
    30841 tcgcagtccc catggtctgc agccagtgag tcaacccatc gagaatgcca gcaaccggtt
    30901 taccgattag gtagatcatc gccagaccga ccaccagact ggaaattagc ggaatgatca
    30961 ggatcggttt cagcgcctcc atactctgtg gcagtttcaa ttgcgtactg attaacttcg
    31021 caatgtaacc agccaggaag ccagcaatta taccgccaat aaagccagaa ccggtgctga
    31081 ccgccagcat accgccaatc agacccggtg tgaggcccgg acgatcggca atggagaagg
    31141 caatataacc tgccagtacc ggcaccatca gcgcaaaggc tgaaccacca ccaatctgca
    31201 tcagcgccgc tgccaacgta cccggctctt taaacgcttc gataccaaaa gcaaaagaaa
    31261 gcgcgataca cagaccacct gcaacaacca tcggcagcat gtaagagacg cccgtcagca
    31321 agtgacggta agcgcctgca ctctctttct taccttcagt ggtcgctgtt tgagctttgc
    31381 ccgctggttc atacggcgtt gcttcagcaa ccgctttatc cagttcctgc gcagttttct
    31441 tcagcgccag accggtagag gtgcgataca tcggtttacc agcaaatttc gccagatcca
    31501 cttcgatatc tgccgccaca atcaccagat ccgctgctgc gacttcttca ggggtgattg
    31561 cattgcccgc gccaacagaa ccacgggttt caactttcac ccaccagcca cgttttttcg
    31621 cttcggtttc aatggcttca gccgccataa aggtgtgtgc tacgccagtc gggcaagcag
    31681 tcaccgcaac tacgcgtttc ggaccgctgg cagcaaccgg tgctgtcgca gcgaccggcg
    31741 cagtgtaagg tttcgcatgg cctttggctt cactcaggaa cagctcaggg tgcgcaactg
    31801 cccgggaaat atcacccagc cagacatttt taccgttcag cgcgctgtca ttcgggatgg
    31861 aatcaccgag aacaatcgcc atttctgcgt cgttcggatt gtcgatgatt tccagttttg
    31921 cttttcgcgc cgccgcgccc agcagggtct tcgccatata ggcgcgtgcc tgaccgagat
    31981 tagcgtcaat aatcagcagc gttttcatta tgcctctcct gctgtcagtt aaaaggttgt
    32041 aagtcgacgc gcgccatcat tgcggccaac tgcggacgat cggtaatacc cacattgctt
    32101 tgacttaccg ccagggctgc aacagctgtc gccagacgca gtgtgtgttc actggattca
    32161 cgcatcagca agccataaat caggccacca accatagaat cccctgcgcc aacggtgctt
    32221 acgacatcga ctgacggtgg tttggcgatc cattcgccgg aggcattaac ccaaagcgcg
    32281 ccttcggcac ccagtgaaat aacaacatgc gcaatacctt gttcacgtag cgcatgtgca
    32341 gcttcaatca catctttcat ttcaggcagt ttacggcctg cccagatttc caactcgcgg
    32401 cggttaggtt tcaccagcca cggtgccgct ttcaaacctg ctactaacgc ttcacggcta
    32461 ctatcaaaga taatgcaagg acactgacta cgcaggcgag tcatccagtc ggtgaacgct
    32521 tccgggctga cgcctgacgg taagcttccg ctgacacaga ccatatcgaa ctgaccgagc
    32581 cagctcagag aatcagtcac aaagcgttcc cagtcggcga gggtgacttc aaaacccgag
    32641 aagttgaagt cggtcacttc gccgtctttt tccgtcagct taacgttaat tcgggtgcgc
    32701 ccctgtacaa cctggaaacg gttggcaatg cccagctcgc tgaacagttg ctgaaaacca
    32761 tcctgattgt ctttacccag gaagccgcca acggtgacat caattcccag gtcttttaat
    32821 actttggcca cgttgatgcc tttacccgcc gcatgcagac cggtggtttt caccaggttc
    32881 acttcgccgc gttcaatttc cgggcagaaa ccaacaaggt cataagccgg attaagggtg
    32941 atcgtagcaa cacgtctgct cattatgcgc cctccccaag accagcagcg atagcgtcgc
    33001 caatggcttt cagcgcctgt tcagcatctg caccctgggc ggtaaagcgt aggcgatgac
    33061 ctttcttaac gccaagtgcc acaactttca tcagactacg tccgtttgcc ggtttgccgg
    33121 tgccatcaag gtttgtcacg gtaatatcac tgttaaattg tttaatggta ttgaccagca
    33181 tggtacctgg acgagcatgc aggccgtgtt cattgcgcac cacaaactcc gcgcttaaca
    33241 cgtcgtcggt cggcgcatca tcgctggtca gcagcgccag caacgttgcc gcatccgctt
    33301 tcagcaagcg gtcagcttta ttgtcgagca ataaatcagc gagacgctta agaaccgcga
    33361 tgggctgatc gtcattcatc gccacactca ccagcatggc tgccgtttcg ccgtccacat
    33421 caaaagcatt tgccgcacgg cttaccgcaa tcgcgctacg cagattgcct tcggcgctat
    33481 cgctcagcca gataccctgt ccgagattca gcggttgttc attgatggct ttggtgacga
    33541 aagtggcgtc aactgccccc acctctttca gacgcgcagc gttcagcgcc tgaagagtca
    33601 gcagatcgct ggcgacgata tccagcgtca gcatttcgtt gtcgagcttc agctgctcac
    33661 tctgcttttc gcccatcagt aatgcgcgaa gttcttctgc tgttgttgct gacttcagtt
    33721 gttcagcaac ggaatcatcg ctcagtacgt gggtcagctg gcgtagcagg cccagatgtt
    33781 catccgagct ggcagcaata ccgattgcca cgtacgctac ctgaccgtca ccccaggtga
    33841 cgccttccgg gaactgaaat acctgaacgc cggttttcag cacctgatcg cgggtgtcgg
    33901 tagtgccgtg tggaatagca ataccattgc cgaggaacgt tgaggtttgc tgttcgcgcg
    33961 ccagcatgcc attgacgtag ccttctgcta cattaccggc ctgcaccagc gccgcagcga
    34021 cctggcgaat cgcctcttct ttgtctccgg ccttttcgcc cggatggatg tcctgtacgg
    34081 ataactggaa catagttctc ctctcttgct gaattgaaac gattcagcct ctatgagaaa
    34141 aaaagcgcca acctggctta gggttaaaga caagatcgcg ctgaaacgtt tcaagaaagc
    34201 ataatacttc tgtttcagca cgcaaggaaa gccgtaaagt tatgagcaaa agtttgatgt
    34261 gctgcacaat tttttgtcat ttttcctaat tgctgatggg aaaatccgtt atcagcaatt
    34321 tcatttcagc agcgtcagca gccaatcagc aacagtgaaa tggcattttg attattcgtg
    34381 gcgcttttga tttcgctgtc tgtttatttt ctgacaagca gcgtaaactc cgcgtcttcc
    34441 tcttccagtg atcgacctgc atgcataact cccccgcagt ctccagcgcg aaatcgtttg
    34501 acctgacctc gacggcgttt ttaatcgttg cctttctcac cggtattgcg ggtgctctgc
    34561 aaaccccgac actcagtatt ttccttaccg atgaagtaca tgcccgtccg gcgatggtgg
    34621 gattcttctt taccggcagc gctgtcattg ggattctggt aagtcagttt ctcgctgggc
    34681 gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt ttgctgcctg ttaggcgtgc
    34741 tggcctgcac cctttttgcc tggaatcgca actactttgt tttgctattc gttggcgtct
    34801 ttcttagcag ctttggctcg accgctaacc cgcaaatgtt tgcccttgcc cgtgaacatg
    34861 ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt tttacgcgct caggtttcac
    34921 tggcatgggt cattggccca ccgctggctt atgcgttagc gatgggtttc agctttacgg
    34981 taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg cgtgatggtg tggctgtttt
    35041 taccgtcgat gcaaaaagag cttccgctgg cgaccggcac ggttgaagcg ccgcgccgta
    35101 accgtcgcga tacgctactg ctgtttgtca tttgtacatt gatgtggggc tcgaacagcc
    35161 tgtacatcat caacatgccg ctatttatta tcaacgaact gcatcttccc gagaaactgg
    35221 ccgatgtgat gatggggacc gccgccgggc tggaaatccc gacgatgttg attgccggat
    35281 atttcgccaa acgtctgggt aagcgtttct taatgcgcgt tgctgccgtg ggtggcgtct
    35341 gtttttacgc aggaatgctg atggcgcatt cacctgtcat tctgttgggc ttgcagctgc
    35401 taaatgctat ttttattggc attctgggcg gcatcgggat gctctatttt caggatctga
    35461 tgcccggtca ggcgggttca gccaccacgc tctataccaa cacttcgcgc gtgggctgga
    35521 tcatcgcagg atcagtggcg ggcatcgtcg ccgaaatctg gaattatcac gctgtgttct
    35581 ggtttgcgat ggtgatgatt atcgccactc tgttttgctt actgcggatt aaagatgttt
    35641 aaggcgcggt cagcatttcc agatcgataa gccaggtcat ggcctgctga cgcgagttac
    35701 cgcacatttc agcgctggct tgtaagcctg cacagacttt cggtcgcagt ggcgaggtga
    35761 aaattttaca gcgttgctgt tcatcaagct gaatgcaggg cgtattggcg ggcttgccat
    35821 ctggcatacc gggaataggg ctggaaattg aaggggcagt gcaacacgcc ccgcaacccg
    35881 gacggcattc catacgttgt tctcttaagg ttaaccaccg gattgatgag cgcgcacagt
    35941 accacctttt ttgcaccagc aaaagtgcga ataccacttg cccgaaaggc ccgtcgcgag
    36001 tactttgtcg cgatattttt gacattttcg actacaggaa tttttcgatg ccaagagcga
    36061 acgaaattaa aaaaggtatg gtactgaatt acaacggcaa actgctgctg gttaaggata
    36121 ttgatattca gtcgcccact gcccgcggtg ccgctacgct gtacaaaatg cgtttttctg
    36181 atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg tgatgatatc gttgacaccg
    36241 tgacgctgac ccgccgttac gttgacttct cctatgtcga tggcaacgaa tatgtcttta
    36301 tggataaaga agactatacc ccgtatacct tcaccaaaga tcagattgaa gaagagttgc
    36361 tgtttatgcc agagggcggc atgccggaca tgcaggtgct gacctgggac ggtcaactgc
    36421 tggcgcttga gctgccgcag accgttgatc tggaaatcgt tgaaaccgca ccaggcatca
    36481 aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt gagcactggt ctggtgattc
    36541 aggtaccaga atacttaagc ccgggcgaaa aaattcgtat ccatatcgaa gaacgccgtt
    36601 atatggggcg tgctgactaa cttcagccgc atgcagaaaa gggatagctc aggctgtccc
    36661 ttttttaatt tattatacca atcttctatt ttgcgcttcc tgaacacctt acgccctcaa
    36721 ttttcactcg ttgattgatc gccctcacac ttcatcgcat taacaatcca gaccaatttc
    36781 aattgctgtc atataacttt acactgtcat tgttaattta tcgttactaa gacgtgactc
    36841 ctatgaagac aattgcctcc gttacgctcc cgcatcatgt acacgctcca cgctacgatc
    36901 gccagcagtt gcaatcacgt atcgttcatt ttggctttgg cgcctttcac cgcgctcatc
    36961 aggcgttact gaccgatcgt gtgctgaatg cccagggcgg cgactggggg atctgtgaaa
    37021 tcagcttgtt cagcggtgat caactgatga gccagctccg cgcacagaac catttatata
    37081 ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat tgtcggtgcc gttcacgaat
    37141 gccttaatgc aaaactggat tccttagcgg caattattga gaaattttgc gagccacagg
    37201 tggcaattgt ttccctgacg attaccgaaa aaggctattg tattgacccg gccaccggtg
    37261 cactcgacac cagtaatccg cggattattc acgatctaca aacccctgaa gaacctcact
    37321 ccgcaccggg tattctcgtc gaagcactga aacgccgccg tgagcgcggc cttacaccgt
    37381 ttaccgtgct ctcctgcgac aatattcccg acaatggcca tgtggtgaaa aacgcggtgc
    37441 tgggaatggc agaaaaatgt tcgccagaac tcgccgggtg gataaaaaaa cacgtcagtt
    37501 ttccgggaac catggtcgac cgcattgttc cggctgcaac cgacgaatca ctggcagaaa
    37561 tcagccagca tctgggggta aatgatccct gcgcgattag ctgcgaaccg tttatccagt
    37621 gggtggttga agataacttc atcgctgggc gtcctgcctg ggaagtcgca ggtgtacaaa
    37681 tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg tatgcttaat ggcagccact
    37741 cttttctcgc ttatctgggt tacctctcag gattcgtcca tatcagtgat tgcatgcagg
    37801 atcgcgtatt tcgccatgct gccagaacat taatgctgga tgagcaagcg ccgacactgc
    37861 aaattaaaga tgtcgattta acacaatatg cggataagtt aattgcacgt tttgctaatc
    37921 cgtcgctgaa acataagacc tggcaaatcg cgatggatgg cagccagaaa ttaccgcaac
    37981 gtatgctggc aggtattcgc atacatctgg ggcgtgaaac ggactggtcg ttgctggcat
    38041 taggcgttgc tggctggatg cgttacgtca gcggcgttga tgatgccgga aatgccattg
    38101 atgttcgcga tccgcttagc gataaaattc gcgaacttgt tgcgggcagc agcagtgaac
    38161 aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg tgatgatctg ccagataacc
    38221 cgcattttgt gcaggccatc gaacaagcct ggcaacaaat cgtacaattc ggcgcacatc
    38281 aggcgctatt aaacaccctc aaaatttaac gatttctgcg gttaaagcgg atgaagctca
    38341 ccttcgtccg ctctcccctt ctcttttctg ccttttttag ccaggattaa cgctcagtta
    38401 acttaccaga gtattgcgga gccgttgtga ccaggaccaa cctcatcacc ggttttctcg
    38461 gcagcgggaa aaccacgtcg attcttcatc tgttagccca taaagatccc aacgaaaaat
    38521 gggcggtact ggttaatgaa tttggggaag tcggaattga tggtgctttg ctcgccgata
    38581 gcggcgcatt gctgaaagag atccccggcg gctgcatgtg ctgcgttaat ggtttaccca
    38641 tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc agaccgcttg ttgatagagc
    38701 cgaccgggct gggccatccg aaacagatcc tcgatctgtt aaccgcacca gtctatgaac
    38761 tgtggataga tctgcgcgcc accttgtgca ttctcgatcc acgcctgctg ctggacgaaa
    38821 aaagcgccag caatgaaaac ttccgtgacc agctggctgc cgcagacatc attgtcgcca
    38881 ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct acagcgttgg tggcagcaaa
    38941 atggtggcga tcgacaatta attcacagtg agcatgggaa agttgacggt catcttctgg
    39001 atttgccgcg tcgcaattta gccgagttgc ccgccagcgc cgcgcattct catcagcatg
    39061 tcgtgaaaaa agggttagca gcgttaagcc tgccagagca tcaacgctgg cgtcgcagtc
    39121 tgaacagcgg gcaaggatat caggcctgcg gctggatatt cgacgctgat acggtattcg
    39181 acaccattgg cattctggaa tgggcgcgac ttgcaccggt ggaacgcgtc aaaggcgtgc
    39241 tgcgtattcc cgaagggctg gtacgaatca accgtcaggg cgatgacctg cacattgaaa
    39301 cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat ttccagcagc gaagctgact
    39361 ggaatgcttt gcagagcgcg ctgttgaagc ttcgtttagc gactaccgcg taaggttgcc
    39421 tgcgtttttc agtaagataa ttagagaaaa tatgattaaa aatttgccgc aaatagtgtt
    39481 gttgaatatt gtcggcctcg cgctgtttct ttcctggtat attcccgtta atcatggatt
    39541 ctggttgccg attgatgcgg atatttttta tttctttaat cagaaactgg tcgaaagtaa
    39601 ggcctttttg tggctggttg cattgaccaa caatcgcgct ttcgacggtt gttctctgct
    39661 ggcgatggga atgttgatgc tgagtttctg gctgaaagaa aacgcccctg gcagacgacg
    39721 tatcgtgatt atgggtctgg tcatgctatt aactgcagtg gtattaaacc agctgggtca
    39781 ggcattaatt cctgtaaaac gggccagccc aacattgact tttaccgata ttaaccgcgt
    39841 cagcgaactg ctctctgttc ccacgaaaga tgcctcacga gatagctttc ccggcgatca
    39901 cggcatgatg ctgcttattt tttcggcatt catgtggcgt tatttcggca aagttgcagg
    39961 ccttatcgcc cttattattt ttgtggtttt tgcatttccc agagtaatga ttggcgcaca
    40021 ctggtttact gacatcattg tcggttcgat gaccgtgata ttgatcggtt tgccctgggt
    40081 gttgctgacg ccattaagtg atcgattaat cacctttttt gacaaatcac taccgggaaa
    40141 aaacaaacat ttccaaaaca aataactcac agtaattaac atcatcaggc ttatttttat
    40201 agtgaggata atcctgatga tgcgcaccgt gctttcatcc atcgcgcgca aaaatcattc
    40261 tctaagtaaa tgaatggatt gcatgcgttt cactcaatcg tactttaatt gaccaatccc
    40321 gcttattaac tttctgtatc actttttctt ataaaaaatc atgtaaaacc gctcgccaag
    40381 accgcaccaa tcgggtaatc tagaactcgt tttgcctcgg cggtagatta tcctcacagc
    40441 atataatttt gtgcgttagt ccacagattt ggccttaagg aattgtttca acatgcccag
    40501 gtaattagtc tcgtgtcgct tggcattttt ttataacgat atttgtcgtt aaggacttca
    40561 agggaaaaca aacaacatgg tcaaatctca accgattttg agatatatct tgcgcgggat
    40621 ccccgcgatt gcagtagcgg ttctgctttc tgcatgtagt gcaaataaca ccgcaaagaa
    40681 tatgcatcct gagacacgtg cagtgggtag tgaaacatca tcactgcaag cttctcagga
    40741 tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg cgaattatgg atcagtatgc
    40801 tgactggaaa ggcgtacgtt atcgtctggg cggcagcact aaaaaaggta tcgattgttc
    40861 tggttttgta caacgtacat tccgtgagca atttggctta gaacttccgc gttcgactta
    40921 cgaacagcag gaaatgggta aatctgtttc ccgcagtaat ttgcgtacgg gtgatttagt
    40981 tctgttccgt gccggttcaa cgggacgcca tgtcggtatt tatatcggca acaatcagtt
    41041 tgtccatgct tccaccagca gtggtgttat tatttccagc atgaatgaac cgtactggaa
    41101 gaagcgttac aacgaagcac gccgggttct cagccgcagc taataaaccg tttggatgca
    41161 atcccttggc tatcctgacg agttaactga aagcactgct taggcagtgc ttttttgttt
    41221 tcattcatca gagaaaatga tgtttccgcg tcttgatcca ggctatagtc cggtcattgt
    41281 tatcttttaa atgttgtcgt aatttcagga aattaacgga atcatgttca tacgcgctcc
    41341 caattctgga cgtaagctcc tgcttacctg cattgtagca ggcgtgatga ttgcgatact
    41401 ggttagctgc cttcagtttt tagtggcctg gcataagcac gaagtcaaat acgacacact
    41461 gattatcgac gtacaaaagt atctcgatac ctattttgcc gacctgaaat ccactactga
    41521 ccggcttcag ccgctgacct tagatacctg ccagcaggct aaccccgaac tgaccgctcg
    41581 cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg aaagataaaa aaacattctg
    41641 ttcatctgcg actggtgaga tggacattcc actcaatgaa ttgattccgg cgctcaacat
    41701 taataaaaac gtcgatatgg cgatcttacc cggcacgccg atggtgccga acaaacccgc
    41761 aatcgtcatc tggtatcgca accctttgct gaaaaatagc ggtgtgtttg ccgctctgaa
    41821 tctcaacctg acgccttctc tcttttatag ttcacggcag gaagattacg atggcgtcgc
    41881 cctcattatt ggcaatactg cgctatctac cttttcttca cgtttgatga acgttaatga
    41941 attaaccgac atgccggtcc gtgaaactaa aattgcgggc attcctctga ccgttcggct
    42001 ttatgcggat gactggacat ggaacgatgt gtggtacgca tttttactgg gcggcatgag
    42061 tggaactgtc gttggcctgc tctgctatta cctgatgagc gtacgtatgc gccccggcag
    42121 agaaatcatg accgccatca agcgcgaaca attttacgtg gcgtatcaac cggtggtgga
    42181 tacacaagct ttgcgagtaa cgggcctgga agtactgcta cgctggcggc atcctgtcgc
    42241 gggagaaatt cccccggatg ccttcattaa ctttgccgaa tcgcaaaaga tgattgtgcc
    42301 gctgactcag cacctgtttg agttaattgc ccgcgatgcc gcagaattag aaaaagtgct
    42361 gccagtaggc gtcaaatttg gtattaacat tgcgccggac catctgcaca gcgaaagctt
    42421 taaagcagat atccagaaac tgctcacttc cctgcccgca caccatttcc agattgtgct
    42481 ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc acacaactct tcgcctggct
    42541 gcactcggtc ggcgtagaaa ttgctattga tgacttcggc accgggcaca gcgcgcttat
    42601 ctatcttgag cgttttacgc tcgattatct gaaaattgac cgtggattta tcaacgccat
    42661 cggtacggaa acgatcactt cacccgtact tgacgcggtg ctgacgctgg cgaaacgcct
    42721 caatatgctg acggttgctg aaggggtcga aacgccggaa caggcgcgat ggctaagcga
    42781 acgcggcgtt aatttcatgc aaggctactg gattagtcgc cctttaccgc tggacgattt
    42841 tgtccgctgg ctgaagaaac cgtatacgcc gcagtggtaa ggtgtgctta cgtcccttat
    42901 tattcatagt gaaagcatgc cggattgcgg ctaatgatga gtaaaaggaa atccattgca
    42961 gatgattgtg cgcatactgc tgctgtttat cgctctgttc acctttggtg tgcaggcgca
    43021 ggctatcaag gaaagctatg cctttgccgt gctgggcgaa ccccggtacg cgtttaattt
    43081 caaccatttt gattatgtga accccgccgc gccaaaaggt gggcagataa cgttgtcagc
    43141 cctcggcacc ttcgataatt tcaaccgcta tgcactgcgc ggcaacccgg gcgcacgcac
    43201 cgagcagctg tacgacacgc tatttacgac ttccgatgac gaaccaggca gttattaccc
    43261 gctgattgct gaaagcgcac gctatgctga cgattattcc tgggtggagg tcgctattaa
    43321 tccgcgcgcc cgttttcatg atggttcgcc cattactgcc cgcgatgtag agtttacttt
    43381 tcaaaaattt atgaccgaag gcgtgccgca atttcgtctg gtctacaaag gcaccaccgt
    43441 caaagccatt gcaccgttaa ccgtgcgcat tgagttagct aaacccagca aagaagatat
    43501 gctgagtctg ttttcgctgc cgatatttcc agaaaagtac tggaaggatc acaaacttag
    43561 cgacccgctc gccacgcctc cgcttgccag tggtccgtac cgcgttacgt cctggaaaat
    43621 ggggcaaaat attgtctatt cccgcgtgaa agattactgg gcagcaaact taccggtaaa
    43681 ccgtggacgc tggaatttcg acaccattcg ctacgattat tacctcgatg ataatgtcgc
    43741 ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg gaaaacgacg ctaaaaactg
    43801 ggccacgcgt tataccggta aaaatttcga taaaaaatac atcatcaaag atgagcaaaa
    43861 gaacgaatca gcccaggata cgcgctggct ggcgtttaat atccaacgtc cggtattcag
    43921 cgatcgccgg gtccgggaag caatcactct cgcctttgac tttgaatgga tgaacaaggc
    43981 gttgttttac aacgcctgga gtcgcacgaa cagttatttt cagaataccg aatacgcggc
    44041 cagaaattac cccgacgccg cggagctggt gcttctggca ccaatgaaaa aagatctacc
    44101 gccagaagtc ttcacacaaa tctaccagcc gccggtatct aaaggcgatg gctacgatcg
    44161 tgacaacctg ttaaaagccg acaaacttct caacgaaacg ggctgggtgc tgaagggtca
    44221 gcaacgcgtt aatgccacaa cgggtcagcc gctcagcttt gaattattgc ttcccgcaag
    44281 cagcaatagt cagtgggtat tgccgttcca gcacagcctg caacggctgg gtatcaatat
    44341 ggacattcgc aaggtggata actcgcaaat cactaaccgc atgcgcagtc gcgactatga
    44401 catgatgccg cgcctatggc gggcgatgcc gtggcccagt tccgatttac agatttcctg
    44461 gtcatcggaa tatatcaatt ccacttataa tgcccccggc gtgcaaagcc cggttatcga
    44521 ctcgctgatc aaccaaatta ttgccgcgca gggaaataaa gaaaaattac tgccgttggg
    44581 gcgagcactg gatcgcgtat taacgtggaa ttattacatg ctgccaatgt ggtacatggc
    44641 ggaagaccgt ctcgcctggt gggataaatt ctcccaaccc gctgtacgcc ctgtttacag
    44701 cctgggtatc gatacctggt ggtatgacgt taataaagcg gccaaactgc cgtcagccag
    44761 acaacaggga gagtagatgg gcgcttacct gattcgccgt ctgttgctgg tgatcccaac
    44821 gttatgggct attatcacta tcaacttttt catcgtgcaa attgcgcctg gcggtccggt
    44881 cgatcaggcc atcgccgcca ttgagtttgg taatgccgga gtattacccg gcgcaggcgg
    44941 tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc aatatcagcg acagtaatta
    45001 ccgtggcgga cgcggattag acccagaagt gatcgctgag atcactcatc gctacggttt
    45061 cgataagccg atccacgaac gttacttcaa aatgctctgg gactacatcc gctttgattt
    45121 tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg attaaagaca gtctgccggt
    45181 ttccatcacc ctcggattgt ggagcacgct gattatctat ctggtgtcga ttccgttagg
    45241 cattcgcaaa gctgtttata atgggagtcg ctttgacgtc tggagtagcg catttatcat
    45301 catcggctac gccattccgg cctttttgtt tgccatcctg ctgattgtct tcttcgcggg
    45361 cggcagctat ttcgacctgt tccctctacg cggcctggtt tccgctaact ttgattcgct
    45421 gccgtggtat cagaaaatca ccgattatct gtggcatatc acgctaccgg tgctagcgac
    45481 agtgattggt ggctttgcgg cgctgaccat gctgaccaaa aactcattcc ttgatgaagt
    45541 gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta agtgagaaaa acattctctg
    45601 gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc ggttttccgg cgacgtttat
    45661 cagcatgttt tttaccggct cgctgctgat tgaggtgatg ttttcactca atggtcttgg
    45721 cttactgggc tacgaagcga ccgtctcgcg cgattatcct gtaatgtttg gtaccttgta
    45781 tattttcacc ctgattggcc tgctgctgaa tattgtcagt gatatcagct atacgctggt
    45841 tgatccgcgt atagattttg agggacgtta atgtcgcgac tcagccccgt caatcaggcc
    45901 cgttgggcgc gttttcgtca taaccgtcgc ggctactggt cgttatggat tttcctcgtt
    45961 ttgtttggtt tgagtttgtg ttctgaactt atcgctaacg ataaaccgtt gctggtgcgt
    46021 tatgacggca gttggtattt cccgttgttg aaaaactaca gcgaaagcga ttttggcggc
    46081 ccgctggcaa gtcaggctga ttatcaggac ccgtggctga aacaacggct ggaaaataac
    46141 ggctgggtac tgtgggcacc gattcgcttt ggtgctacca gtatcaactt tgctaccgat
    46201 aagcccttcc cttctccacc ctcccggcaa aactggctgg gaacggatgc caacggcggc
    46261 gatgtgctgg cacgcattct ctatggcacg cggatctcgg ttctgtttgg cctgatgctg
    46321 actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc tacaaggcta ttacggcggt
    46381 aaagtcgatc tctggggaca acgctttatt gaagtatggt cggggatgcc gacgctgttt
    46441 ttgattattt tactttccag cgtcgtacag cctaacttct ggtggctgct ggcaattact
    46501 gtcttgtttg gctggatgag tctggtcggc gtggtgcggg cggagttttt acgtactcgt
    46561 aatttcgact acattcgtgc ggcacaggcg cttggcgtca gcgatcgcag tatcatcctg
    46621 cgtcatatgt tgcctaatgc catggtcgcg accctcacct ttttaccgtt tattttatgt
    46681 agttcgataa ccaccctgac ctcgctcgat ttcctcggct tcggtctacc gctcggttca
    46741 ccgtcactcg gtgaactgct gttacaaggg aaaaataacc ttcaggcccc gtggcttggg
    46801 atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc tgatctttat tggtgaagcc
    46861 gtccgcgacg catttgatcc taataaggcg gtgtagcatg acgcaaactc tgttagcgat
    46921 tgaaaatttg tcggtgggtt ttcgccatca gcaaaccgta cgtacagtag tcaatgatgt
    46981 ttcactacag attgaggctg gcgaaacgct ggcgctggtg ggtgagtcag gttcaggcaa
    47041 aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc ccgccggttg aatatctctc
    47101 cggcgatatt cgttttcatg gcgaatcgct gcttcatgcc agcgatcaaa cgttgcgcgg
    47161 tgtacgcggt aataagatcg ccatgatttt tcaggaaccg atggtgtcgt taaatccatt
    47221 gcataccctg gaaaaacagc tttatgaagt gctttcactc caccgcggga tgcgtcggga
    47281 agcggctcgt ggcgaaattc ttaactgcct tgatcgcgtc ggtatccgcc aggcggcaaa
    47341 acggctgaca gattatccgc atcagctctc cggcggcgaa cgccagcggg tgatgattgc
    47401 gatggcgctg ttaacgcgac cggaattatt aattgccgat gaaccgacca ccgcactgga
    47461 cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa ctgcaaggcg agctgaatat
    47521 gggcatgctg tttattactc ataacctcag cattgtcaga aaactggccc accgcgtggc
    47581 ggtaatgcaa aacggtcgct gtgtcgagca aaataacgcc gctacgctat ttgcctcacc
    47641 cactcatcct tacacacaaa agctactcaa cagtgaaccg tcaggcgatc cggtaccgtt
    47701 gccagaacct gcctcaacgt tgctggatgt tgaacagctt caggttgcct tccccattcg
    47761 caaagggatt ttgaagcgca ttgtggatca taatgtggtg gtgaaaaaca tcagttttac
    47821 gctacgggcg ggtgaaacac tgggtttagt gggcgagtct ggttccggga aaagcacgac
    47881 gggactggcg ctgctgcgac tgattacctc tcagggcagc atcgtctttg acggtcagcc
    47941 actgcaagat ttaaatcgcc gccagctgtt acctactcgt catcgtattc aggtggtatt
    48001 tcaggatcca aactcctcgc tcaacccacg actcaacgtt ttgcagatta ttgaggaagg
    48061 cttacgggtt caccagccga cgctttctgc cgcacaacgc gaacaacaag tgatagccgt
    48121 gatgcatgaa gtgggattag atcctgaaac acgccaccgt tatccggcgg agttctctgg
    48181 tggtcagcga caacgtattg cgattgccag ggcattaatt cttaagccct cactgatcat
    48241 acttgatgaa ccgacatcat cactcgacaa aacggtacag gcgcaaatat tgacgctatt
    48301 gaaatcattg caacaaaagc atcaactggc ctatttgttt atcagccacg atttgcacgt
    48361 tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa ggggaagtag tggaacaagg
    48421 accgtgcgcg cgcgtgtttg ccgcaccgca gcaggagtat acgcgtcagc tactggcgtt
    48481 gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc ggcaattgcc acaccaaaat
    48541 ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt tacaaacagg cacggctcac
    48601 cttcacactc cagcaccgaa catggaactt cgatgtcgct taacgcctgg cttagtttat
    48661 acatcaccga ccatgcatta tcaccatccg ggctaagcaa tttaagcgcc accaggctgt
    48721 tgtcaccgca cggtccattt tgcggaatcg gttcaacctt cgaacctgcg aaaccagcag
    48781 accagcgaaa gttctgcggt agacgatgga cgattgagag ttgtaatgaa gtcacttatt
    48841 ttccccggaa gcacatttac ttcacaattt atttacatca attttaacac atcatcaaca
    48901 atccgtcttt atacagatcg taaagattga tgcctcgcct gcatggcctc tatggctctg
    48961 atttaagtaa tatctgcggg cgtggtcacg tttgcggggc tatatgtacc cgtttctgcg
    49021 atctaactca acctttttaa ctacaatgat gtgacttttt acataaattg attttacata
    49081 aaataaacat atatcggggg aatgatagat ttgtggttga catgcatcaa caaaggaagc
    49141 cttttagctt cctcgttgtg caatagatca ccgttttttc ggtcgactgg cgtacagaca
    49201 gaagagaatg gagctggttg cgcagaatgc aattgaccaa atcatcggcc atgcagagtt
    49261 aaaggtcgca agagaaagca atgcgccaac aattgcccct atgccaaaac ggaaggttcc
    49321 tgccagcgaa gatgccgttc ccgccatatg gggaaactca tcaagaatga ccgccatcgc
    49381 attggatgac accatagaca cgcagcccac aaacgccgca acgccaacca ccagcgacca
    49441 gaatcccagc cccagcagcg cactgatgac catccacgct gccataataa attgtatcca
    49501 caaccccgag cggaacatat ttaacgcgcc aatgcggcgg acgaagcggc tgttaaagat
    49561 ggtcatcacg aacagaaaaa caatgtttag cgcaaagtaa taaccaaagt tttccggtgc
    49621 aacgtggttg atttcaatgt agacaaacgg tccggcgctc aggaacgaga acattccggc
    49681 aaagctgaaa ccgctggcca gcatataact caggacacgc ttatgacgga acagcgccgc
    49741 aaagttacca atagtggtac gaatgtgaaa aggctggcga ggatccagaa gatgtaatgc
    49801 cagctcagcc acaccagcac ccagccgcca actatcggtg ccatcagcgg tgcaatggtc
    49861 gtcaccagca tgacaaacga catcatccgc gagaactctt ctttcgggta aatatcacgc
    49921 atcaaagcgt tgataaccac gctggccgca gccgcagcca gcccgtggaa gaaacgcatc
    49981 acaatcagct gatcgatggt ttgtgccaac gcacacgcca ccgcggcggc ggcaaacacc
    50041 agcgtaccgc caagaaccac cggcttacgc ccgaagctgt ctgccatcgg cccgtagatt
    50101 aactgcccca acgcaaagcc cagaatataa gtactcaggg tcatctgcgt actgcccgcc
    50161 ggtacgccaa actgcgctga aattaccggt agcgcgggca gatacatatc aatcgacagc
    50221 ggcatcaaca tggccagcag gccaaggata aaaacaatag caaacgacga atgctgtcgg
    50281 gtggtcacaa cgggctcctg aaagtcattg aaaagttaga cgacgctggc aatttcttct
    50341 tcagttaacg gacgatattc accgggggct aaatcagcat ccagcgtaat gccgccaata
    50401 cgttcacgat gcagctcaac cacatggtta cccacggcgg cgaacatgcg tttcacctga
    50461 tgataacgcc cttcgctgat ggtcagacga acctgcgttg gggtaatcac ttccagcacc
    50521 gccggcttag tgagatcttt ttcgttatgc agctgcacgc ctttagcaaa ttgttctgcc
    50581 gtatcgtcag ctacgggtga taccagcgtc accagatagg tcttctcgca atgatggcgc
    50641 ggagaagtaa tgcggtgcga ccactgacca tcatctgtca ttagtaccag accggtggta
    50701 tcgatatcca gccgccctgc ggcgtgcagt ttccacgcta ccggttcatc tagaaaatag
    50761 agcaccgttg ggtgatcagg gtcgtccgtg gagcaaacat aaccctgagg cttattgagc
    50821 atgaagtaac gtggaccgtg ttgctgcgcc agcgggttgc catcgtaagc gacatcatgt
    50881 tcaggaagca gtttgaacgc tgcattacgg acgatttcgc catcgacggt gacacgattg
    50941 ccgcggattt cacgcccggc aatagcacgg ctaacgccga gttgctgtgc gataaattta
    51001 tcaagtcgca tgtgtgtgat tttgcctgta aaaaacggag gtcgggtaat gacccgaata
    51061 tctgaactgt tgtctgccca gtatagcggt ctaataacat ccctcaaggg aaaaagatcc
    51121 gtggcatact attagcagaa taatctacct acgcgagacc atgattttta cacttcgccc
    51181 atatcagcaa gaagccgtgg atgccacgct caaccatttt cgtcgtcata aaacccctgc
    51241 cgttatcgtg ctgcccaccg gtgcaggtaa aagcctggtg atagcggaac tggcacggct
    51301 ggctcgtggt cgcgtactgg tgctggcaca cgttaaagaa ctggtggcgc aaaaccatgc
    51361 aaagtatcag gcgctggggc tggaagccga tatttttgcc gccgggctga agcgtaaaga
    51421 gagccacggt aaagtggtat ttggcagtgt gcagtcggtc gcccgtaata ttgatgcctt
    51481 tcagggtgaa ttttcgctgt tgattgtcga tgaatgtcac cgtattggtg acgatgaaga
    51541 gagccagtat cagcaaatcc tcactcacct gacaaaagtg aatccccact tacgcctgct
    51601 ggggctgact gccacgcctt ttcgattggg caaaggttgg atctaccagt tccattatca
    51661 cggcatggta cgcagcgatg agaaagccct tttccgtgac tgcatttatg agctgcccct
    51721 gcgttatatg attaaacaca gctatctgac gccgccagaa cgactggata tgccagtagt
    51781 gcaatatgat ttcagccgct tgcaggcaca gagcaacggg ctgttcagcg aagccgatct
    51841 caaccgtgag ctgaaaaaac agcaacgtat taccccgcac atcatcagcc agattatgga
    51901 gttcgccgaa aagcgtaaag gagtgatgat tttcgccgcg acggtcgaac acgcaaaaga
    51961 gattgtggga ttactgcctg ccgaagatgc agcactgatt actggcgaca cccccggcgc
    52021 tgagcgcgat gtgttaattg aaaattttaa agcccagcgt tttcgctatc tggtcaacgt
    52081 cgcggtactg accaccggat ttgacgcccc gcacgtcgat cttatcgcca ttctgcgccc
    52141 taccgaatca gtgagtcttt accaacaaat tgtcgggcgc ggtctgcgtc tcgctccggg
    52201 caagactgat tgtttaattc ttgattatgc gggtaatcct cacgatctct acgcgccgga
    52261 agttggtaca ccgaaaggca aaagtgacaa cgttccggta caggttttct gccctgcctg
    52321 cggttttgcc aacacctttt gggggaaaac gaccgccgac gggacattga ttgaacactt
    52381 tggtcgccgc tgtcagggat ggtttgaaga tgacgacggt catcgcgaac agtgtgactt
    52441 ccgtttccgt tttaaaaatt gcccgcaatg taacgcagaa aatgatattg ccgctcgccg
    52501 ctgccgcgaa tgtgacacgg tactggttga tccggatgat atgttaaaag cggcgctacg
    52561 actgaaagat gcgctggtat tacgctgtag cggcatgtct ttgcagcatg ggcacgacga
    52621 gaaaggcgaa tggttgaaaa tcacctatta cgatgaagac ggtgcggatg tgagtgagcg
    52681 tttccgtctg caaacacctg cccagcgtac cgccttcgag cagcttttta tccgcccgca
    52741 tacgcgcaca ccgggcatcc cgctgcgctg gatcaccgcc gccgatatcc tcgcccagca
    52801 agccttattg cgacccccgg attttgtcgt cgcccgcatg aaaggccagt actggcaggt
    52861 gcgtgaaaaa gtgttcgatt acgaaggtcg ttttcgtcgg gcgcacgaat tacgcggtta
    52921 atggtagttt tgattgatgt ataaggcgat tgagtataga atctcacccg cttttgcata
    52981 cgcaaagcag atcacttacc tgttgctggg tcgcctgtag caggattaat ttaagagaga
    53041 aagaaatgtt tactatcaac gcagaagtac gtaaagagca gggtaagggt gcgagccgcc
    53101 gcctgcgtgc cgctaacaag ttcccggcaa tcatctacgg tggcaaagaa gcgccgctgg
    53161 ctatcgagct ggatcacgac aaagtcatga acatgcaagc taaagctgaa ttctacagcg
    53221 aagttctgac catcgttgtt gacggtaaag aaatcaaagt taaagctcag gacgtacagc
    53281 gtcacccgta caaaccgaag ctgcagcaca tcgacttcgt tcgcgcttaa ttgctgaata
    53341 agttgtaaaa aaccccgctc ccgcggggtt ttttgtatct gcagattatg cctgattacg
    53401 gtattgctat tttttgcagg ccagataagg cgttcacgcc gcatctggca ttcagaacac
    53461 aaaaccgatt aattgccgcc agacgtccgg cgctgcaatt ggtcgcgcaa attcggcggc
    53521 gtgcctttaa tggtcagggt atcggtcgcc ggctcccaga agatgcgttc acctaacagc
    53581 atcgcatcaa agttaatagt caagccaccg ccgctaccgg caaacttcgt caactgacgc
    53641 agtgtgctcc ggtctgccgg gaagctctct tccagttcgt agcctttttc agccgcaaac
    53701 tccgtgaagc tcacttcgct tacgcctgcc agctctttcg acagcgattc cagttcgatc
    53761 tcttcgcctg cctgcagctg ctcattacag tagctataaa cctgctggcg cacgttctgt
    53821 cgctctgctt tatccagttg tgcttcggca gtgaaatcat caaccgcttg caacagccca
    53881 cggttttgtg ctttcgtgtt caatccttcg ctggccccga ggaaatccat aaagaaatcc
    53941 gccactttgc gccctactcg ccctttcagg aaagtgagat agcgggtgga ctctggattg
    54001 gtttcccatt cggttaaatc gatacgcgca acaatatccg catgattgat atcaagataa
    54061 tgggttgggt tgatgtcgag attttcgtta acacgcatac tgctcaggtt gctcagaact
    54121 gccaccagca gatactccac cgccagataa cggtagtggc aaaacagcac gaatccaccg
    54181 tcagcaaaag gatatttcgc taattcgtca cgcaggcgac cagttgccgc gcggctaaat
    54241 gccagaaagt cctcttcgcc ctgacgctgt aagcgcagcg tctgcgccag ttcactctct
    54301 tcgctaaaca agccgtaagc tttatttttg gcgctataga cccgatgcag ttctgccacc
    54361 atctcaacga cggtttctgt cggttccagc aatgaatcgc gcaacaccag ctcaaggttc
    54421 tgctcatcac gcttgataag ctggtgcaga gcaatctggt tgatatccag actcatgata
    54481 aactctcctt taagaccggg cggtattcaa ccaccgccgg ttaagcgacg caataaaaga
    54541 tggcggtcat tttacagaag gcagcgtaac gagaatgatt taacgcataa aaaagcagaa
    54601 aaaaaaccgt tgctacggta atatgttgcc ctttcatcaa ctaactgatt tcgatttatg
    54661 ccacaaattt cccgctacag tgatgaacag gttgaacaat tgctcgcaga gctgctcaac
    54721 gtactggaaa aacataaggc tccgaccgac ctttccctga tggtgttagg caacatggtc
    54781 actaacctta tcaacaccag cattgccccg gcccaacgcc aggcaattgc caactctttt
    54841 gcccgcgcct tacagtcctc tatcaacgaa gacaaagcgc actaagggaa acagataaca
    54901 ggttatggta actcatcgtc agcgctaccg tgaaaaagtc tcccagatgg tcagttgggg
    54961 gcactggttt gcactgttca atattctgct ttcgctcgtc attggcagcc gttacctgtt
    55021 tatcgccgac tggccgacaa cgcttgctgg tcgcatttat tcctacgtaa gcattatcgg
    55081 ccatttcagc ttcctggtgt tcgccaccta cttgctgatc ctcttcccgc tgacctttat
    55141 cgtcggctcc cagaggctga tgaggttttt gtccgtcatt ctggcaacgg cgggaatgac
    55201 gctattactg atcgatagcg aagtctttac tcgtttccat ctccatctta atcccatcgt
    55261 ctggcagttg gttatcaacc cagacgaaaa tgagatggcg cgcgactggc agctgatgtt
    55321 catcagcgtg ccggttattt tattgcttga actggtgttt gcgacgtgga gctggcaaaa
    55381 gctgcgcagc ctgacgcgtc gtcgacgctt cgcgcgcccg ctggccgcat tcttatttat
    55441 cgcctttatc gcctcgcatg tggtgtatat ctgggccgat gccaacttct atcgcccgat
    55501 caccatgcag cgcgctaacc tgccgctttc gtacccgatg acggcgcgac gttttcttga
    55561 gaagcatggt ctgcttgatg cgcaggagta tcaacgccgt cttattgagc aaggtaatcc
    55621 agacgccgtt tccgttcagt atccgttaag cgaactgcgc tatcgcgata tgggcaccgg
    55681 gcagaatgtg ctgttgatta ctgtcgatgg cctgaactac tcacgcttcg agaagcagat
    55741 gcctgcgctg gcaggttttg ctgagcaaaa tatttcgttc acgcgccata tgagctccgg
    55801 caacactaca gacaacggca tctttggcct gttctatggt atctcgccga gctatatgga
    55861 cggcattctg tcgacccgta cgcctgcggc attaattact gcgcttaatc agcaaggcta
    55921 tcagctgggg ttattctcat cagatggctt taccagcccg ctgtatcgcc aggcattgtt
    55981 gtcagatttc tcgatgccga gcgtacgcac ccaatccgac gagcagaccg ccacgcagtg
    56041 gatcaactgg ctgggccact acgcacaaga agataaccgc tggttctcgt gggtttcttt
    56101 caatggcact aacattgacg acagcaatca gcaggcattt gcacggaaat atagccgggc
    56161 ggcaggcaat gtcgatgacc agatcaaccg cgtgctcaat gcactgcgtg attctggcaa
    56221 actggacaat acggttgtga ttatcactgc cggtcggggt attccgctga gcgaagacga
    56281 agaaaccttt gactggtccc acggtcatct gcaggtgcca ttagtgattc actggccagg
    56341 cacgccggcg cagcgtatta atgcgctgac tgatcatacc gatctgatga cgacgctgat
    56401 gcaacgcctg ctacatgtca gcacacctgc cagcgaatat tcgcaaggtc aggatttgtt
    56461 caaccctcaa cgccgtcatt actgggttac tgcagcggat aacgatacgc tggcaattac
    56521 caccccgaaa aagacgctgg tgctgaacaa taacggtaaa taccgcactt acaacttacg
    56581 tggtgaaaga gtgaaagatg aaaaaccaca gttaagtttg ttattgcaag tgctgacaga
    56641 cgagaagcgt tttatcgcta actgattaat tataaatcag ttagcgaaat atcttacttg
    56701 caatcggtgt ggaaaacggt agtattagca gccacgagtc ggcacgtagc gcagcctggt
    56761 agcgcaccgt catggggtgt cgggggtcgg aggttcaaat cctctcgtgc cgaccaaaaa
    56821 tcccaagaaa aaaccaacct ttacggttgg tttttttata tctgcaatta atttgataaa
    56881 cagaccgtga cacatcacag cctgtttatt ttctgttatc agaacgtcca gaccactccc
    56941 gcctgagtat tccacggcga ttctacccct gcgccattgc catagctgac tgacaaatga
    57001 ccgcttaacg tcggggtaaa cgatgacctt atcccagcct gataaacgcc acgggttcca
    57061 gccacatcat tattaaattt gccatcgtca ttaactttca catggttaga atcggcgtat
    57121 tcctgacgca cggcggcttt cagccagggt tccaacgtcg taccgttttg caggtccatg
    57181 tgatagctta ccgccgttcc cgcttcagcg cgtaatatcc gggtatttcc cacatccgcg
    57241 cgcatgccgt ttgataacgt gtagtcctga ccatctgtgg taaagccggt aaaggcgaga
    57301 tagggtctaa cactccacaa tccgtcaacc caacggaacc cgctctcaac atgagcaccc
    57361 gcgccgttac tattgtaatc gccaaacgct gttgccccat tactcatctt gccatggatg
    57421 gtgttggcaa aacggtcaac tttcaccacc ccatcaacat aggcaccgtt ctgatgctcc
    57481 caaccggcat aagcccccag ggtgtagcta tcaatattgc ctttgccgcc gcgatcaaaa
    57541 ccaatatcag aatgagagta atcaaagaac aagccgcgaa ttgtgctgct ttcttcacgg
    57601 gagaaacggc tatcgatacc gagcgtcagg cccgtcaatg tttgctcaaa gccagctccc
    57661 gcatcagtgg tcacgttgtt gcgggtgtta attgccgaac tccacatcgc cgtatcgtaa
    57721 ttaacgcctt ttacgctacc aagacgctca cgcacggtgt ccagttctgc atcaaatacc
    57781 agcggttgtg ctgctgccat gttcagcaca tcagtggttg aaggggtaat ttgcgcgcga
    57841 ttctctgcga ggctccagct atggttgcca ttatccagca aggtatattc atacgtaccg
    57901 atatcaacaa cgcctccggc attgcccaac gtgaatgcag catcaccgcc gcccgtcgtt
    57961 accagtgtaa ggctatctcc tgctgccggg ctggcccccg tgtccgtcac gaatattttg
    58021 aaatcacctg ttgcctgacc gatgacgttg agctgatcgc tctgatgatt agccatatcg
    58081 gtacgcatat aaaattgccg tttcccgaca gggtattggt ggtcaacgta atgaaatttt
    58141 ccgcacttgc cgtcacagat gaacgagtca ctggctcagc catcataatc acatttgcgc
    58201 cggacatcgc aaggttggct aagttgaggc gagtgttttg gttcgtcgta gtgatatcgg
    58261 caatgatggt ccatgcgcta ttttcgagcg aaacgtccgc ttggctggta tccacggcac
    58321 catgcgttct aaaagaagcg ccctcagaaa ccactacctt acccaccaac atagcgggtg
    58381 cataatttat tttcggcttc atgacctcaa gaatgccatc attccctctg actgacacgt
    58441 taaccattgt gccagcccag acattagcgc ggccattgtt gataaccata ttttcagaga
    58501 tggctttaga ggaatacgtg atacttccat tctgatctga tcgaccgaga tcatatacag
    58561 catttgcctg cactttcgta ccagtatcct ttcccagcga ttgcatagtg gcatgctcat
    58621 caaggatagt gtcaatggcc tgagtgtcct ccataacaat gagcccggag ccatcatcca
    58681 gttcataatt ttttgacaca ccatccttta tactaaattg gcctttactg tttgtaccac
    58741 tcacttccag cgcattcgtt gagacaatta atttcccgcc tgctttttta tcaacgccag
    58801 ttgcagtccc gccatccata acctccagta atcctccctt ttcgatagcg gcaaagacgg
    58861 caatcccatc tttttcaaca attaagcgcc cgcctttaat cgtgggatca ttcgccgcg
//
LOCUS       DAZPJR010000006        58387 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPJR010000006 DAZPJR010000000
VERSION     DAZPJR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.007"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHVB7_000379"
     CDS             <1..161
                     /locus_tag="ACHVB7_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR1912476.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="ACHVB7_000380"
     CDS             complement(199..1260)
                     /locus_tag="ACHVB7_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1912477.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="ACHVB7_000381"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="ACHVB7_000381"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR1912478.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="ACHVB7_000382"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="ACHVB7_000382"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR1912479.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4155)
                     /locus_tag="ACHVB7_000383"
     CDS             complement(3454..4155)
                     /locus_tag="ACHVB7_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR1912480.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHVB7_000384"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHVB7_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR1912481.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHVB7_000385"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHVB7_000385"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR1912482.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="ACHVB7_000386"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="ACHVB7_000386"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR1912483.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="ACHVB7_000387"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="ACHVB7_000387"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR1912484.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHVB7_000388"
     CDS             8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHVB7_000388"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR1912485.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10493..12034
                     /gene="ppx"
                     /locus_tag="ACHVB7_000389"
     CDS             10493..12034
                     /gene="ppx"
                     /locus_tag="ACHVB7_000389"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR1912486.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHVB7_000390"
     CDS             complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHVB7_000390"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1912487.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14498..14650)
                     /locus_tag="ACHVB7_000391"
     CDS             complement(14498..14650)
                     /locus_tag="ACHVB7_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912488.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14668..14859
                     /locus_tag="ACHVB7_000392"
     CDS             14668..14859
                     /locus_tag="ACHVB7_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR1912489.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15170..15688
                     /locus_tag="ACHVB7_000393"
     CDS             15170..15688
                     /locus_tag="ACHVB7_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1912490.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15704..16243
                     /locus_tag="ACHVB7_000394"
     CDS             15704..16243
                     /locus_tag="ACHVB7_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR1912491.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16338..17915)
                     /gene="guaA"
                     /locus_tag="ACHVB7_000395"
     CDS             complement(16338..17915)
                     /gene="guaA"
                     /locus_tag="ACHVB7_000395"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR1912492.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17984..19450)
                     /gene="guaB"
                     /locus_tag="ACHVB7_000396"
     CDS             complement(17984..19450)
                     /gene="guaB"
                     /locus_tag="ACHVB7_000396"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR1912493.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19612..20982
                     /gene="xseA"
                     /locus_tag="ACHVB7_000397"
     CDS             19612..20982
                     /gene="xseA"
                     /locus_tag="ACHVB7_000397"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR1912494.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20979..21194)
                     /locus_tag="ACHVB7_000398"
     CDS             complement(20979..21194)
                     /locus_tag="ACHVB7_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR1912495.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21263..22735)
                     /gene="der"
                     /locus_tag="ACHVB7_000399"
     CDS             complement(21263..22735)
                     /gene="der"
                     /locus_tag="ACHVB7_000399"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR1912496.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22853..24031)
                     /gene="bamB"
                     /locus_tag="ACHVB7_000400"
     CDS             complement(22853..24031)
                     /gene="bamB"
                     /locus_tag="ACHVB7_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR1912497.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24042..24662)
                     /gene="yfgM"
                     /locus_tag="ACHVB7_000401"
     CDS             complement(24042..24662)
                     /gene="yfgM"
                     /locus_tag="ACHVB7_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR1912498.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24680..25954)
                     /gene="hisS"
                     /locus_tag="ACHVB7_000402"
     CDS             complement(24680..25954)
                     /gene="hisS"
                     /locus_tag="ACHVB7_000402"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR1912499.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26065..27183)
                     /gene="ispG"
                     /locus_tag="ACHVB7_000403"
                     /gene_synonym="gcpE"
     CDS             complement(26065..27183)
                     /gene="ispG"
                     /locus_tag="ACHVB7_000403"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR1912500.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27210..28223)
                     /gene="rodZ"
                     /locus_tag="ACHVB7_000404"
     CDS             complement(27210..28223)
                     /gene="rodZ"
                     /locus_tag="ACHVB7_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR1912501.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28508..29662)
                     /locus_tag="ACHVB7_000405"
     CDS             complement(28508..29662)
                     /locus_tag="ACHVB7_000405"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR1912502.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29812..30243)
                     /gene="ndk"
                     /locus_tag="ACHVB7_000406"
     CDS             complement(29812..30243)
                     /gene="ndk"
                     /locus_tag="ACHVB7_000406"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR1912503.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30392..32704)
                     /gene="pbpC"
                     /locus_tag="ACHVB7_000407"
     CDS             complement(30392..32704)
                     /gene="pbpC"
                     /locus_tag="ACHVB7_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR1912504.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32705..37666)
                     /gene="yfhM"
                     /locus_tag="ACHVB7_000408"
     CDS             complement(32705..37666)
                     /gene="yfhM"
                     /locus_tag="ACHVB7_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR1912505.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNCLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37873..38718
                     /gene="sseA"
                     /locus_tag="ACHVB7_000409"
     CDS             37873..38718
                     /gene="sseA"
                     /locus_tag="ACHVB7_000409"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR1912506.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            39054..39179
                     /gene="timP"
                     /locus_tag="ACHVB7_000410"
     CDS             39054..39179
                     /gene="timP"
                     /locus_tag="ACHVB7_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR1912507.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(39211..39987)
                     /gene="sseB"
                     /locus_tag="ACHVB7_000411"
     CDS             complement(39211..39987)
                     /gene="sseB"
                     /locus_tag="ACHVB7_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR1912508.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40129..41412)
                     /gene="pepB"
                     /locus_tag="ACHVB7_000412"
     CDS             complement(40129..41412)
                     /gene="pepB"
                     /locus_tag="ACHVB7_000412"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR1912509.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41590..41790)
                     /gene="iscX"
                     /locus_tag="ACHVB7_000413"
     CDS             complement(41590..41790)
                     /gene="iscX"
                     /locus_tag="ACHVB7_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR1912510.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41802..42137)
                     /gene="fdx"
                     /locus_tag="ACHVB7_000414"
     CDS             complement(41802..42137)
                     /gene="fdx"
                     /locus_tag="ACHVB7_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR1912511.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42139..43989)
                     /gene="hscA"
                     /locus_tag="ACHVB7_000415"
     CDS             complement(42139..43989)
                     /gene="hscA"
                     /locus_tag="ACHVB7_000415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR1912512.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44006..44521)
                     /gene="hscB"
                     /locus_tag="ACHVB7_000416"
     CDS             complement(44006..44521)
                     /gene="hscB"
                     /locus_tag="ACHVB7_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR1912513.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44617..44940)
                     /gene="iscA"
                     /locus_tag="ACHVB7_000417"
     CDS             complement(44617..44940)
                     /gene="iscA"
                     /locus_tag="ACHVB7_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR1912514.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44957..45343)
                     /gene="iscU"
                     /locus_tag="ACHVB7_000418"
     CDS             complement(44957..45343)
                     /gene="iscU"
                     /locus_tag="ACHVB7_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR1912515.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45371..46585)
                     /gene="iscS"
                     /locus_tag="ACHVB7_000419"
     CDS             complement(45371..46585)
                     /gene="iscS"
                     /locus_tag="ACHVB7_000419"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR1912516.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46826..47314)
                     /gene="iscR"
                     /locus_tag="ACHVB7_000420"
     CDS             complement(46826..47314)
                     /gene="iscR"
                     /locus_tag="ACHVB7_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR1912517.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47615..48352)
                     /gene="trmJ"
                     /locus_tag="ACHVB7_000421"
     CDS             complement(47615..48352)
                     /gene="trmJ"
                     /locus_tag="ACHVB7_000421"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR1912518.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48471..49274
                     /gene="suhB"
                     /locus_tag="ACHVB7_000422"
     CDS             48471..49274
                     /gene="suhB"
                     /locus_tag="ACHVB7_000422"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR1912519.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49419..50273
                     /locus_tag="ACHVB7_000423"
     CDS             49419..50273
                     /locus_tag="ACHVB7_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1912520.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50464..51744
                     /gene="csiE"
                     /locus_tag="ACHVB7_000424"
     CDS             50464..51744
                     /gene="csiE"
                     /locus_tag="ACHVB7_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR1912521.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51736..52875)
                     /locus_tag="ACHVB7_000425"
     CDS             complement(51736..52875)
                     /locus_tag="ACHVB7_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR1912522.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53035..53925)
                     /gene="hcaR"
                     /locus_tag="ACHVB7_000426"
     CDS             complement(53035..53925)
                     /gene="hcaR"
                     /locus_tag="ACHVB7_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR1912523.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            54061..55422
                     /gene="hcaE"
                     /locus_tag="ACHVB7_000427"
     CDS             54061..55422
                     /gene="hcaE"
                     /locus_tag="ACHVB7_000427"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR1912524.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55419..55937
                     /gene="hcaF"
                     /locus_tag="ACHVB7_000428"
     CDS             55419..55937
                     /gene="hcaF"
                     /locus_tag="ACHVB7_000428"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR1912525.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55937..56257
                     /locus_tag="ACHVB7_000429"
     CDS             55937..56257
                     /locus_tag="ACHVB7_000429"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR1912526.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56254..57066
                     /gene="hcaB"
                     /locus_tag="ACHVB7_000430"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56254..57066
                     /gene="hcaB"
                     /locus_tag="ACHVB7_000430"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR1912527.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57076..58278
                     /gene="hcaD"
                     /locus_tag="ACHVB7_000431"
     CDS             57076..58278
                     /gene="hcaD"
                     /locus_tag="ACHVB7_000431"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR1912528.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14272 a        15785 c        14565 g        13765 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttgtgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
     8341 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
     8401 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
     8461 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
     8521 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
     8581 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
     8641 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
     8701 ctctacaacg agctgctgct ggagatggcg cgtaaccaga tcttcctgat taatgaacgc
     8761 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
     8821 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
     8881 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
     8941 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc
     9001 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
     9061 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
     9121 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
     9181 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
     9241 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
     9301 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     9361 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     9421 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     9481 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     9541 taccgtgtgg caaaagattc acgtatcatc gactcgatga tccacgctgc gcacaacggc
     9601 aagaaagtga ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     9661 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     9721 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
     9781 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     9841 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     9901 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
     9961 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
    10021 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgtggcctcc
    10081 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
    10141 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
    10201 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
    10261 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
    10321 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agctcgttat
    10381 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
    10441 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
    10501 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
    10561 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
    10621 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
    10681 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
    10741 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
    10801 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
    10861 tctgattttt atgggcgtgg aacataccca accggagaaa ggtcgcaaac tggttattga
    10921 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccga ttctcgttga
    10981 aagccgccgg atgggttgtg tcagctttgc ccagctttac ttccctggcg gggtcatcaa
    11041 taaagagaat tttcagcgcg ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg
    11101 gcaattccgt attcagggct ggaacgtggc aatgggcgct tccggtacca taaaagccgc
    11161 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
    11221 aaaactggta aaagaagttt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
    11281 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgcg gtgtgtttga
    11341 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
    11401 cgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
    11461 caaccagtat cacatcgaca gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat
    11521 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
    11581 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
    11641 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
    11701 gatgatggcg acactggtgc gctatcaccg taaagcgatt aaactcgacg atctgccgcg
    11761 ctttaccttg tttaagaaga aacagttcct gccactgatt cagctattgc gccttggcgt
    11821 attactcaac aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga
    11881 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgttggtact
    11941 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
    12001 tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
    12061 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcaacg tatcaattaa
    12121 cggctgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
    12181 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
    12241 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
    12301 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
    12361 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
    12421 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
    12481 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
    12541 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
    12601 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
    12661 accaaattcg tgcgcgaccg gcaagaagct atcggggacg atcagttcat cattctcacc
    12721 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
    12781 catcaggaaa aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt
    12841 atctttcaat tcgcgttgca aatacattgc cccgcgacgc tgcatatttt ccggggcgtt
    12901 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
    12961 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
    13021 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
    13081 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
    13141 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg tattgaatcc gcagcatgat
    13201 gccatagttc ttaaccagca tttccatgcc agggatgcgt aaataacaaa gcgcagacca
    13261 gggagcatca cgcaacgcgc gattcagggc gcgaacattt ggcagatgaa ccaccgggtc
    13321 catatacgca agccgctgaa tgcgtctgac taccgctctc tgacgggttg ccagtacagc
    13381 catataattg acaataaaag agaataccag ataactggag gaggttatgg tcagctgcgt
    13441 ggtatagcca ggataaatgg gaatgtaatt ttgatagctg tggatgctga tcatcaacac
    13501 gaccgcccag agcagcgaaa tcagcttata accatagcgc atcgctcccc acatcatcag
    13561 gggcagcaat aatgacaagg tataattggt gctaaaaatt gtgctttttt catttaacgg
    13621 catgcataac agcaacaata aagcacctaa tgccagtagc cagagcgcga actctttttt
    13681 ggtgactttg gcatcaacct gctgttttaa ttgcgaataa tagctacgta aataaaatgg
    13741 atttcgcact acccgaatga tgaagtagca cagcgggaca ccgatcagat tacccaccag
    13801 taaggcctga taattgatta aagtcccgag gttaaaaggc atgacaccga ccagattttc
    13861 tctgctcgcc agtaatccta caaatgcagc aaactggaaa agtatcagaa acagcgttgc
    13921 aggaaacaca atctgccaga atatacgctg ggaaattaaa cgggtatcac catgtgaaac
    13981 gttgttacgc cggggagtaa agaccctgta accgccccaa cagagaatga taatgataat
    14041 aaaattagcc gttaatgata gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa
    14101 aatccctaag gcgatccctg gcaatgccgc ccagctgaaa aacatcatca tgctgatcat
    14161 cagtgccaga ggcagataaa agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt
    14221 aatgtgggcg aaaatgggca agattaaaga aggcaggaac agcggaagcc cccaccattt
    14281 atcacgcatt tttatataag ttgcattcag tttcatagat gctcagcaga atcccccaca
    14341 tcctgaagga ggtgtattca gacaggcatc ccacctgact tcgaatgatg attattcatc
    14401 actatagaga gcattgattc taagtgtcat atgaaagttc caattgatat atatcaaaca
    14461 aaataaccct gattaatgaa ttattacgtt tatcatgtta attcatcatt attacatcat
    14521 cattgtaaat aattaaatta acttccataa cattaaaata tgtatccact gacgtttttt
    14581 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
    14641 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
    14701 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
    14761 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
    14821 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc ttcactttca
    14881 cagaacataa cggcacttcg ctgtcggatg cttttgctct ttgggattat caaagcggca
    14941 gatattcttt catcttaaat tttacgtctt tgtcctgact gatgtttatc ctgtttggct
    15001 gcggaataaa tataaaatta atatatatgt tgtaatgata tatttttata aattattccc
    15061 tgcgtgaatt ttaataaatt taatctatcc ctttatacgc aatacattta ctttcctctt
    15121 ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt
    15181 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc
    15241 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
    15301 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
    15361 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
    15421 cggccacaac gtcggttctg gcagcaattc cggaacgacg gcaggggcag ttggcggcgg
    15481 agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
    15541 tttaacatat aaggaaggca ccaaagtgta tacctccacc caggtgggta aagagtgcca
    15601 gtttacgaca ggtttagccg ttgttattac cacaacgtat aacgaaacgc gtattcagcc
    15661 aaatactaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct
    15721 ttgcgccatc ttagcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact
    15781 ctctgctgtc gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga
    15841 atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca
    15901 ggccgccgct aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa
    15961 actggacgcc gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg
    16021 cagcttagag attcagaaac aaaaactgac gctggcgaaa gaagaagccc gcgttaagcg
    16081 agaaaacgaa tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca
    16141 atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt
    16201 gggcaaagca gaagagaaca aatcggacag ctggtttaat taatcgatgt tagtaacttc
    16261 aagcctatga ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca
    16321 tagtgcaggg attaaaatca ttcccactca atggtagctg gcggcttgcc gctgatgtca
    16381 tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac gcgaccgagg
    16441 aaatcgtacg gcagacgcgc ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc
    16501 agagagacaa cccagtcata cttacgacca tcgcccatta cgccaacgga acgtaccggc
    16561 aggaacacag tgaacgcctg gctgactttg tcgtacaggt ccgctttacg cagttcttca
    16621 atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac ttcacccagc
    16681 acacgaacgc caaggcctgg tcccgggaat gggtgacggt acaacatgtc gtacggcagg
    16741 cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag cggttcaacc
    16801 aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt gatgacgtgt
    16861 gctttaccgg ttgcagacgc cgcagattcg ataacgtcag ggtagatggt gccctgcgcc
    16921 agccacttca cgtcttccag tttcagcgct tcttcatcga atacttcaac gaaaacgcga
    16981 ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga caggaagcga
    17041 tcttctgccg gaacgtgaac aatgttcaga ccaaagtgat cgccaaacat atccagaacc
    17101 tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt caggttttta
    17161 ccgatagcgc ggtgcagtag cattgcggtt acggaggaat ccacgccacc agagaggccg
    17221 aggatgactt tatcgtcgcc tacctgctca cggatgcggg ctacagcatc gtcgataatt
    17281 ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg ctccagcatg
    17341 cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata gaagcgtttt
    17401 tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt gacgaagtcg
    17461 gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag cggtttaccg
    17521 tctgcggtca gcgcatcttc gataccgcga accagtgcac tgtcgtttac gacttcaacc
    17581 tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc
    17641 atggtctgca tgccatagca aacgccgaat accggtacgc ctgcttcaaa gacatactgc
    17701 ggcgcacgcg ggctgttttc ttcagtagta ctttccgggc cgccggaaag aataatgccg
    17761 cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa
    17821 acacccagct cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc gaagtctaga
    17881 atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga ggcaagtgaa
    17941 acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag cccagacggt
    18001 agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga atgcccgcac
    18061 cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca cagccggtca
    18121 gacccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc aggcgacctt
    18181 tataagctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg ttatcgctct
    18241 ggaaataacg gtcagaggaa cctttggaca tcgcgcccag ggaacccata ccacggtaag
    18301 atttgtaaga acggccctgg tagagttcga tttcacccgg agattcttca gtacccgcca
    18361 gcatggaacc taccatcacc gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg
    18421 agaagcgaat accaccatct gcgataaccg gaataccggt gccttccagg gcttcaactg
    18481 cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt aacgatacga gtcgtacaga
    18541 tagaaccagg gccgataccg actttaaccg cgctgcaacc agcttctgcc aaagcgcgtg
    18601 cacctgcagc tgttgccacg ttgccgccga tgatttgcag atccggatat ttagcacggg
    18661 tttcacggat acgctgcaaa acgccttctg agtgaccgtg ggaggagtcg atcagcagaa
    18721 cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc gttacccgca cctgcgccaa
    18781 ctgcagcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt ttacgttccg
    18841 ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca
    18901 gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg gcttcacctt
    18961 cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc tggttcaggt
    19021 cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt tcttcggtaa
    19081 cgaccggata gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg cgcagcgtcg
    19141 tggttggcag aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc
    19201 gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg ataccgcctt
    19261 cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct gcggaaagca
    19321 tagggatatt cagacgaata gttttcgtca gctgggtgct gaggtcagca gtattcggca
    19381 gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga
    19441 tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc ggcattatac
    19501 agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc cccgaagggc
    19561 gggttactat cgactgaata acctgctgat ttagaatttg atctcgctca catgttacct
    19621 tctcaatccc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg tctgctgctt
    19681 gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac acaaccggct
    19741 tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg cgcgatgttc
    19801 cgcaacagca accgccgggt gaccttccgt ccacagcatg gacaacaagt tttagttcgc
    19861 gccaatatta cgctctacga gccgcgcggt gactaccaga taatcgttga gagtatgcag
    19921 ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa gttgcaggct
    19981 gaaggtttgt tcgatcagca atacaaaaaa ccacttccct cccctgcgca ttgcgttggt
    20041 gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc
    20101 gatccttctc taccggtgat catctacccc accgccgttc agggcgatga cgcaccgggg
    20161 caaattgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt
    20221 gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg cgtagcgcgg
    20281 gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc
    20341 attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg
    20401 agccgtaatc agcaagagtt actgcgccag gtgcaatcga cccgtcaacg gctggagatg
    20461 gcgatggatt attatctcgc caaccgcacg cgtcgcttta cgcagatcca tcaccgatta
    20521 cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga gcgcctgcaa
    20581 aagcggatga gctttgcgct ggaaaatcaa cttaagcgta ccgggcaaca gcagcagcgg
    20641 ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc gcaaacgcgc
    20701 attcagcaac tggaatatcg tttagcagaa accctgcgcg tacagcttag cgccacgcgt
    20761 gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc aacgctggcg
    20821 cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt gaagcaagtt
    20881 aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag tgaagttaaa
    20941 aacatccagc cagtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg
    21001 cgttttttcg atatcaagcc atgcccgtgc tgacagaagt aatctactgc accgcaggct
    21061 tttaacacct gcaacggttg atggcagtct ggacaaagag ctttcatttc aataatttcc
    21121 ccgcaggatg ggcaacgcgc atggccgtta tcctgatcaa gcacatgctg acattgtgga
    21181 caatgcagtt ccatatgaat tcctccggta gccatccctc tacattcata gagggaatgg
    21241 cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc agacgcttac
    21301 gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg ttttcccctt
    21361 ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg aagtagttca
    21421 tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga atcaccacaa
    21481 tcggcgggtt ataaccacca gcgtgggcat atttcagctt cacacgacga ccgcgtacca
    21541 gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata gaggtcccca
    21601 cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta ccaacaccac
    21661 tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc agacggaagt
    21721 ccagcgtttc tttcacctgc tctttcactt cctggctcag gccatcccac ttattcacca
    21781 caatgacaag tgagcgccca ctattgagaa taaagcccag cagcgagaga tcctgatcgg
    21841 aaataccttc gcgcgcatca atcactaaca tcaccacgtt ggtatcttca attgcctgca
    21901 acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt ttacgtacgc
    21961 cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattggaatg tagatactgt
    22021 cacgcgtcgt gccaggcatg tcataaacaa caacgcgctc ttcaccaaga atacggttag
    22081 tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc ggcagacttt
    22141 gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca aattgcgccc
    22201 agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc cacggcagca
    22261 gcacacgctc cagcagactt aatacgccac gaccgtgaga tgcggcgatc gggtagattt
    22321 cacctaaacc aagcgagtag aaatcaacca ctgcctgatc gggatccaga ccgtcagttt
    22381 tgtttgccac caggaaggtc ggtttttcac gagagcgcag atgtttggca atcgcttcat
    22441 ctgccggcat caagcccgcg cgcgcatcca ccataaacag tacgacgtcc gcttcttcaa
    22501 tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg ccatcaatcc
    22561 cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg tacttacggt
    22621 cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt agacggttaa
    22681 ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt accatgttta
    22741 aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt tgttaaaaac
    22801 aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac gattaacgtg
    22861 taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca gcggcaaccg
    22921 gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga ccatcttcga
    22981 cgttaatcca gtgcagataa ccttcactgt cacctaccac caggttgcca ttatacagca
    23041 ccggcgaagt cagcaggcga tgcagcagat cgctttgtgt ccacagcgta acgccgccat
    23101 caatggtcaa cgccatcacc cggtcatttt gatcgaccag atagatgcga ttgccgtcga
    23161 cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca ctgcgcagat
    23221 caagcgccgt caggttacca ttataggcca gagcgaaaac aacgccgtta acaacgacgg
    23281 gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc tgggaaatac
    23341 gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga ccattatcgc
    23401 cccccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc aaagagagcg
    23461 aaggcatatc gaggttaact gtccatttga cagcgccgtc agcttcgttc agcgcttgta
    23521 actgaccgtt actagtgtgg attaacacca gaccgtcgct gaccaccggg cgagaaagtg
    23581 cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc agcgcgtaaa
    23641 cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaca ccgccagaaa
    23701 gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca gaccagattt
    23761 ctttgccatc atccgcattc agcgctttta ccaaaccagc gcggtccgct gcatagacaa
    23821 cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg ctaccaacgg
    23881 aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat ggggacatct
    23941 ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta acggaaagca
    24001 gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga caaattatta
    24061 attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac gcctgcttcc
    24121 catgcactac gcgcaccttg cttatcacct ttgctcaaca atgctttacc acgcaggtcg
    24181 gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag cgcggcatca
    24241 gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc tttgagattt
    24301 tcatcactcg tgtctgccag cccctgttgt aactgggcgg cagctttctc tagttcattt
    24361 ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt atttttattt
    24421 tcagcagcaa atttttccgc cgccgggatg ctatccggtt tgccttcgct cactgcggta
    24481 accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg gctgttccag
    24541 tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag tgctttgcca
    24601 ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc gtaaatttcc
    24661 acgctgtcct tctccttcct taacccagta acgtgcgcaa atgcgcggct acgctatcct
    24721 gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg ccgttagcca
    24781 cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca cgggcaaact
    24841 gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt aattcatcac
    24901 gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa gccaccaggt
    24961 atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgtact aacaatacaa
    25021 gacgttcgag gcccatcgca aaaccgactg ccggtgttgc acgaccgccc agttgttcca
    25081 ccagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgagg ctgttagtca
    25141 cccactcgaa aacggtacgg ttatagtaat ccagaccacg caccagacgc tggtttacgg
    25201 tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc tcacgagatt
    25261 cctcgtccag ataatcacct aatgccggag cgtcgttgag aagcgcctgc acttccggat
    25321 tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag tcttcgtcca
    25381 gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg gcgcgtgctt
    25441 ccagcgaacc aatagagttc agctcaagag ttacgtgttc gaagataccc agcgcacgcc
    25501 accagcgggc agtgagcata atcagttcag cgtcgatatc cggaccttgc agaccgaaaa
    25561 cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc tcgtgacgga
    25621 acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca tgctcgatgc
    25681 cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg ccattgcgat
    25741 cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc gcacgtttga
    25801 atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag ctgccgagca
    25861 cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc aggtaatcgt
    25921 tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg aatataaaaa
    25981 tgaaccctca acgcttccct caatgtttcg ggagccatgc gggttcaatc atacacggga
    26041 agcgaggcgc ttcccatcac gttattattt ttcaacctgc tgaacgtcaa ttcgacgcgc
    26101 ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc agctggtcga tcatatcgtt
    26161 gttgtccaga cggtctttgc gcacgccatc ttcatagagg ccgcttttct tgttgccgcc
    26221 ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc agccgataat
    26281 cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt taaccgtacc
    26341 gataacatca aattcctgac gcgaacaggt cgggcaggcg atgaagttaa tccctcgcga
    26401 acggatacgc agcgatttca aaatatcgaa accgactttg atctcttcga ccggatcggc
    26461 cgccagcgat acgcgcagcg tgtcgccgat gccttccgac agcagcagac ctaaaccaat
    26521 ggcggatttt actgccccgc tgcgtgcacc accggcttcg gtgatcccca gatgcagcgg
    26581 ctgatcgatc tgttttgcca gcaaacgata agactcaaca gcgaggaaga cgtcagacgc
    26641 tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa catgacgcat
    26701 ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca gatctttttc
    26761 cagcgatccg gcgttaacgc caatacggat cggaatgttt ttatcgcgcg cacagtcaac
    26821 caccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca gacaatcgac
    26881 gccgtattcc gctactttca gcgcaatgcg atagtcgaag tggatgtcag ccaccagcgg
    26941 cacgttaacc cgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg tcggtacgga
    27001 gacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga ccgttgcttc
    27061 gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac catcgccaat
    27121 cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag cctggttatg
    27181 catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc ggcattgagg
    27241 gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg tttcccttga
    27301 tactggatct gtactgcggc tggcgcacca attttcagtt tatacggtgc ctggccggtt
    27361 aaattcaaat taccgtcttt acgctgcatc ccgctaaaca attttttacc ggtagcatca
    27421 gtgacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg atcagccgcc
    27481 ggcgtggtca cgccagcctg atcggttggc aacggcgcag caccatctgg cgttgttgct
    27541 gccgttggta ccggggtcgc ggcggtatca acatttgcct gcgaaggcga aacaaccgca
    27601 ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg cgtgttggtt
    27661 gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt agttgtcgac
    27721 gtatttaacg ggacactctg cccctgctcg ctattactgc tcagttctgc cgaagattga
    27781 tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca ccaggcaccg
    27841 ctcaggccga taaccacaaa caacaccagc caggtgaagg tcatcagcca gccatcacgt
    27901 tttttgcggc gtttaccgag ggaaaaactc tgcattggcg caacttttgc agcccgtagt
    27961 ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac cagacgcgca
    28021 taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc cttatcttct
    28081 tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc ctgctgacta
    28141 agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc ttcattttgg
    28201 tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat tgagatgccg
    28261 gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt tatcatacag
    28321 tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa cttactgttg
    28381 catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga ccagacaatt
    28441 gcaacttaat acttcattca taaaacatta cgccacggta cataaagtaa ccgtggcgta
    28501 atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta cgcagggtac
    28561 gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatcg atatcatcac
    28621 cacgagtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt gagaaacggt
    28681 cgatacggct gttcgagctg cgtccatacg gcgcacccgg gaacgggttc cacgggatca
    28741 ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg tgttcagtgc
    28801 cgtcgttaac gtggtcaagc atcacgtatt caatagtgac tcggccctga ttggcgttgg
    28861 atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac tttttgttga
    28921 tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt gccagtgcaa
    28981 cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagta gaaagcgtga
    29041 cgcgacgttt agacaggcca aaaccgaaat catcaagcat gatttccatc gccggaacga
    29101 cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta gtgattggcc
    29161 gctgaccggt gacttttgct gcgccgacga ttttcgccgc ccgccacacc tggccgataa
    29221 tttccgacac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag aatttacact
    29281 ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct tccgggatat
    29341 acaccgtttc gacgcgctga tcgccaacgg cgatcgccca tttaatggtg ccgtcggatg
    29401 aacgctgttc ttcaaccact tccggtgcgc ggatctccgc cacctctttc agtttgccgc
    29461 gcaacacttt gttgatgtcg gtcatctcat caaagttgtc gcagcaatag tgatacatcc
    29521 acttcatcac ctgatcggcg cggaagggtt tttcacctaa atctttaaaa aactcccgca
    29581 tctgctgacg gttgagatcc agcaggttga tttttccatc tttcgtggtg acgttttcag
    29641 gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt tatggcccct
    29701 ggagggttga aaaaagaaac gccccggtga gcggctgctc gtccgggggc gctgcattgt
    29761 acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt attaacgggt
    29821 gcgcgggcac acttcgcctt cgccgaagaa ataagcgatt tcgcgagcgg cagattcgac
    29881 ggaatcagaa ccgtgggtac cgttttcggt caggctgtca gcgtaatcag cgcgcagagt
    29941 accggccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac gctgaacggc
    30001 gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt caaccagacc
    30061 atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgagcctgtt caacggtcag
    30121 gtgcagcatt ttggtgccga caattttgaa ccctgcagct tcaaagcgcg caaagatatt
    30181 accaatgacg ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac gttcaatagc
    30241 catttttacc tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag atggcgcgga
    30301 ttataatgag caacagggcc gttgactatt gatgaaggta acattttttt aaaataaaat
    30361 gagttttagc aacaatcagg gacaaaacag actattgcat gacaaatttc actgtcgcga
    30421 tttgccccac gtcatccatc accagcaatt gataatcacc tttatccgtc aaatgcaggg
    30481 tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag cgttcacctg
    30541 cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa cgtttaataa
    30601 tcgcgccatc gcgaacgcct gtcagctgca gagggagctg ggcatcgtga ccgtatggcg
    30661 ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg ggcagccagg
    30721 gttccagcgg tagcggccag acatttacca tttcttgctt cgcctgcggg cagtcagcgg
    30781 cgacgcgctg ccctttgtca tccagccaga tggggaagcg aatgccatta atcccttcct
    30841 gctctgacag taacagcgtt ggcggctggc tgccatccag cagccaggtt gccaggcggc
    30901 ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcag ataacgccac
    30961 gagtgactga gttcgggcgc gggtcttccg gcagattcgc actgcgcgac agtaagatat
    31021 tattgacctg attcaacaat ggtacggcac tggcaaagcc aaactgacca acaacgggcg
    31081 tgccgtcagg tctgccagtc cagatcccaa tgacatagcg agcgttaacc ccaatcgccc
    31141 aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg acgcgcggca
    31201 aggcactatc cggcaagggt tgcgcttcat cagccataat ccggcgaatg atccacgccg
    31261 cccccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag cgcaatttgc
    31321 ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc agttttgcac
    31381 cagcgccgcc gagaatgagt gaaagattcg gcgcagcgcc gttgggcaaa tataacggca
    31441 atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc agcacctgca
    31501 cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat gctgatcggg ccatgaaaac
    31561 cgctatcaaa gttacctggt cgataatcac cggtgcgccg ggggacgtct tgcagcagtg
    31621 atgccgggtg gatcaagcct tcatccagcg ccagaccata aacaaacggt ttgagcaccg
    31681 atcctggcga tcggatcgca ttgaccatat caacatgacc aaagcgtgaa tcatcgttga
    31741 gatcaaccga tcccacccag ccgcgaacac gcatatcggt atgatcaacc acgatcatcg
    31801 ccagtgagct gcgcggtggc aaccgccctt tccagttttg cgccagttct tccagacgtc
    31861 gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta ccgagcatca
    31921 tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag atgggttctt
    31981 cccttgactc ttttacctgc tcacgggacc acacaccttg cgcagccatc cgttcgagca
    32041 ctttattacg cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga cggctgggcg
    32101 cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca ggcgactttc
    32161 cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac ggagcgcggt
    32221 taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc aacgcgcgcc
    32281 agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga cgagcaacct
    32341 gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct tgccatgctg
    32401 cgcgcgccac cgagaatgga ttcacccccg gatgcttcca gaaccagcga tcttcataat
    32461 tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga taacgccaga
    32521 tgccgtcagc atcggcgaag cgccagagcg gcgtaccatc ctgcgccacg accactcgtg
    32581 cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac gctgcaagaa
    32641 aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt aacaagcgag
    32701 gcatttacgg tcggacaatc agcagatctt cagccgcgcc ggtcgcccgc cattggggaa
    32761 catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc accgcccgcg
    32821 ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc acaaagcgat
    32881 cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt aagttctgca
    32941 cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctgg ttttccagtt
    33001 ccagacccac aggcagcaga tccacgacta acgcatccgg cacgctgtta ctggctttta
    33061 cctgcaacca caccagcacc agatcgccgc tacgtaacga gtccagcgat ttgctcttac
    33121 catcagtacc aagaatatga cgctcgattt gcagcacatt gttcgccggt aaaggtgcgg
    33181 attgcggata accgctggca tccatacgca accataacgg ctgatcgcca ctgttgctca
    33241 cctgcaaggt gacaagttga tcgctattca gattgctgtt ttgcgctttc tcgcctgtca
    33301 gcggctcagc tgagaaagag gtttgcgcct gccatttacc cggtaaatcc tgaatcgtac
    33361 gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca ccaaacgcct
    33421 gctgcgaaag tgtgttcagc aaagtgtact gctcatcggg tagcagttta ttttcttcca
    33481 gcaaggagag cattaacgcg ttgtcgcgca gtggactacc gtaatcaccc agccatatcc
    33541 gctcatcact atttctcggc gttttcagcg ccagcgcaat cgcttcttca ccacgcgtcg
    33601 catcacccat ggttttcagc gcaatgccaa gttgcagcag cggtaaacca gaagcggcat
    33661 ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg tgctttttgc tgacgggcca
    33721 gcaccagcgc agcgtaagac tgaacggcga atttactggc tttgagatta tccgcgtacg
    33781 ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta ccccggttaa
    33841 tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa tccatcacgt
    33901 aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag ccgccgttat
    33961 cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg cgtttctcat
    34021 cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa agtgacggga
    34081 acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac tctttgatat
    34141 aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc agcgtaacag
    34201 gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc tgtaacgccg
    34261 taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc cactgcttat
    34321 gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc tgaatttctc
    34381 catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc acacctggcg
    34441 ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg gcggtcaggg
    34501 caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc agacgcgagg
    34561 tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt gcggcaacta
    34621 tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca ggcttgcgcc atgacgcgca
    34681 attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt tcgttgagcg
    34741 ttaccgacag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt ttaccaccac
    34801 gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc tgaccttcaa
    34861 taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca aagaacgcct
    34921 gccacggatc tggcgtaacg tagtcagtaa tattcagcac accactatca acggcagaca
    34981 ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta attttcacgg
    35041 ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg aggcgacggt
    35101 tttcatcacc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc gcggagcgag
    35161 atttatcgcc aggacgcacc accagcgtac tcaaataaag atcatgacga ttccaggttt
    35221 tatcgaccgg aatcgtcaga tccagccctt gagccggaac atcaatctct tgccaccaca
    35281 gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc gcggcaatat
    35341 gcaacttaat ggtatcgccg gggcgataac tggctttatc tagtttcagc gtgacacggt
    35401 cgggtcgcac tgcgccgctc ccgtcgctgt tgtcctgcca gctatagcca gcccaaaaac
    35461 gcacgctact gaccgcttca ttcggcgctt tgacttccag acgataagcg ccccactcta
    35521 ccgggaaact gaccttaccg gtttcatccg cctgaagatc cagagtttgt tcattttcga
    35581 tcagatcttt ttgatcaaac tgtgactgcc agccttcatc ttctgaccag ttccagtagt
    35641 aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt ttcacgcctt
    35701 gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca atgggctgtt
    35761 tcaccgtgct gtcagtgcga taatcgtaaa ccgatttcaa ggcgaactgc ggacggagcc
    35821 ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc gggcgaccgc
    35881 ccgattccag caaactgccc tggaaaataa cctgcaatgg ggaatgcgtt tccttccact
    35941 ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga acttcatcca
    36001 gcgtgcggga aaggttttcg gcagctatat cgccgaattc aaaaccaggt aaggctgaca
    36061 cagcctcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca ttagcaggcg
    36121 caccgtacag gtagtatccc accacggaga atttcacttc atcattcggc gtaagcgggg
    36181 ttttctcacc ggtcagattc agcgccatac gctctggcat aaaatcttcg acgtggaaat
    36241 cccacatccg gtactggtta tcgcccgtgt tggcgcgaat atgccacata ccggttgccg
    36301 cattgctatc gagtggccag gtaaagtggt agaggccatt ctccggctga ctaacgacgt
    36361 tcctgagtac ctgcccgtca ggtttaatca cgtctaactt gatgggttga tcgggcaatg
    36421 ctttaccgtc tgcatcgcgc agtaaaccat tgaggattac cgtttcacct gggcgataaa
    36481 gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca gcaatgttaa
    36541 attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga ccgtctttac
    36601 gtgccagcag taatgccgca tttttatcat tttccagctg cacatgcccc tgagcgtcac
    36661 tggttgcctg agtcagagtc tgtcctttct catttaataa agagacttca attccttgct
    36721 gggccgcgcc gttttccaga ctttgggtaa agatatccag acaattgtga taacggtgag
    36781 ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa tcgtaacgtc
    36841 cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg atatcgccca
    36901 gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac cgtccggtgt
    36961 agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc gaattgcggt
    37021 attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag aagttaacat
    37081 cgacattatt aacgttgagc gccattaccg gcagcccttc aacgactttg ccaggcagca
    37141 gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc gcgggtagtt atagtttttt
    37201 cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca atagtaacga
    37261 tcaaatcacg tttcggttcg aggtggcgta aacgtagctc tttaagatta tctgacagct
    37321 cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg cgtgagaaat
    37381 cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca ccatcaagtt
    37441 ggacttcaga gagatccagc aatgtcagca ccttaccggc actctgttgg gccagttttt
    37501 gtctctccgg cacggagagt tttgctgaac tcgcgttttc tgaagaggcc gctttagtaa
    37561 cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg tcgcaccctg
    37621 ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt catccctgcc
    37681 acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg ttatgtaatt
    37741 cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac agccaaatca
    37801 gcctctttcg cttgtcgctt ttcactaaac atccgacaat ttagcctaac cccggcaaaa
    37861 atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc cgaacatatt
    37921 gatgacccgg aaattcagat tatcgatgcc cgcatggcgt cgcctggaca ggaagatcgt
    37981 aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt tgatatcgaa
    38041 gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga aacgttcgcc
    38101 gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta tgacgaaggt
    38161 aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt agagaaagtg
    38221 tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgctgtt agaagaaggt
    38281 gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc cgtggtgaaa
    38341 gtaaccgatg tattattggc aagccatgaa aatacggcgc aaattattga tgcccgcccg
    38401 gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg cggacatatt
    38461 cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg aaggcgaact aaaaacgacc
    38521 gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct acgacaaacc aattatcgtc
    38581 agctgcggct ctggtgtaac ggcagccgtg gttttgttag cactcgcgac gctggatgtg
    38641 ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc agatttaccg
    38701 gttgagccag tgaaataagt attttacagg caataaaaaa ccgccgaatt tggcggtttt
    38761 ttattgctgg tctggttcgc ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg
    38821 cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa agagagttat
    38881 tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca agcgggcgct
    38941 ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg
    39001 cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga
    39061 tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa gtaaataata
    39121 accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc agcaagtaag
    39181 cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt taaagtcacg
    39241 caaaaaacca ccccagcgac gttcatagaa tggcacaata tgttcggtaa taaagtggct
    39301 aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc caggtaatgt
    39361 atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat cggcttcaat
    39421 gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa taaaagcacg
    39481 cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg
    39541 ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct ggctgctcag
    39601 cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt ctttaccggt
    39661 cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca atgtgcgaac
    39721 gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt
    39781 gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat caagcgcact
    39841 atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg tggattccag
    39901 tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc
    39961 ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt tcggggcgca
    40021 aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt tgaatttgca
    40081 gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt acgccgttaa
    40141 cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact gttcaaccgg
    40201 cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat agttctcaac
    40261 aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt
    40321 attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg ccagcggcag
    40381 acgccagaat ggttcgttct cttgtgaggc actcgccagc aaacgaccgg caagcgcatc
    40441 gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg ccccggtgag
    40501 ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca gaccatcggc
    40561 aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac cgttgcgata
    40621 ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg cacagcagag
    40681 gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca aatgccagcg ccccggtaac
    40741 cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct gtttgatgct
    40801 gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca ctggcgcttc
    40861 tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg
    40921 tccgacggtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt tggtgatccg
    40981 ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct gtgccagttg
    41041 cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat
    41101 caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact ccactttacg
    41161 cgtgcctttc ggggctttgt aaccttgcca gaatgcccag cagcgatccg catcccagcc
    41221 ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg
    41281 gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat tattaatgct
    41341 gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat
    41401 cgcttctgtc attttagtta tccttctttg taaaagggcc gccagttggc agccccagga
    41461 tttttccgct aaatgattcg cgttgcagaa aggcggcaag tgagtgaagc cccaggagca
    41521 tagataacta tgtgactggg gtgaacgagc gcagccaacg catctgcggc gtgaagcatg
    41581 acgcggaaat tattcggcct cgtccagcca gactaacaaa atcgcttcga gaattttctc
    41641 gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg caaatccact gatgcatatc
    41701 ggtgaatcga accgttttcg gatcaagatc aggatacgca tcgtacagtg cttcgccaat
    41761 ttcgcggcta tcggtccact taagtcccat actaacctct gttaatgctc acgcgcatgg
    41821 ttgatagtgt aacgcgggat ttcgactact aaatcttcgt cggtaacacg tgcctggcag
    41881 cttaaacggc tttcgggctc cagtccccag gctttgtcca gcatgtcgtc ttcctgctct
    41941 gagctttctg gcagcgagtc aaaaccttca cgaacgatgc agtggcaggt ggtgcaagca
    42001 caggattttt cacaggcgtg ttcaatctcg ataccgttac gcagagctgc gtcgagaatg
    42061 gtttcaccgc tattagcttc cagaacagcg ccatcaggac agagatcctg atgaggcaaa
    42121 ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg cacgacgaac
    42181 cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc
    42241 ttgttcgatg gcgtcaacgt catcgccctg cgccacttca ctcaggtgag cggcagcatc
    42301 gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca tcagcagcca gcgcgccgtg
    42361 cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcattc gggcttttac
    42421 gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag
    42481 accgtaagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct ccatcgccgt
    42541 cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg cgaatatgcg caccaccagc
    42601 cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt gagcggcagt cctgcaccag
    42661 ttcgcgctca ccctgcatta catggataga catcgccgtc tgaccatctt taaaggtggt
    42721 gaaatcctga gcgcgggcca ccggaatagt ggtattacgc ggaatcactt tctccaccag
    42781 gccgcccatc gtttcgaggc ccagcgacag tgggatcaca tcaagcagca gcatttcgct
    42841 gtctggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt
    42901 atccgggtcg atggaagtca gcggtggacg accgaaaaat tcgcctaccc gttcacgcac
    42961 cagcggcacg cgagtagaac cgcccaccat caccacttcc agcacttcgt cagcttctac
    43021 acccgcgtct ttaagcgcgc gacgacaagc cagtaaggtt cgttttacca gtggcgcgat
    43081 cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacgt taacggtcac
    43141 ggagtccgca tcgctcagcg cgattttggc tgcaatggcg gcatccagca gttcacgctg
    43201 aacgcggtta tcgctacgat caggaatgcc cgcctgctcg cgaatgtaat ccgccagcag
    43261 atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gttgccagca cttcaaacac
    43321 gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccaccgaggt cataaacagc
    43381 gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcgg tcggttcgtt
    43441 aagtaagcgc agaacatgca accccgccag acgcgccgcg tctttggtgc cctgacgctg
    43501 ggcatcgtca aagtacgccg gaacggtgat aactacacca tccagctcgc ctgccagggc
    43561 ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca ccgggttcag
    43621 cagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga attgataagg
    43681 cagatgcgga tagcgttgct ggatatcagc cagcgagcgc cccatcaggc gtttaacaga
    43741 actaatggtg ttggcggtgt caagcgctgc gttagtacgc gcgtcataac ccaccgaatg
    43801 cccttgctgt tgatagtgaa caacagatgg cagcaggtga cggccttcat ggtcggctaa
    43861 cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat
    43921 accagccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac taatttgtaa
    43981 taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg agttgttcgg
    44041 cactgctccg cagtttatcg agaaaacgca gcttacgcac ggtatccgcc gccgcgtccc
    44101 acgcctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac atctttttca
    44161 cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc tcgtccagct
    44221 cttcgcgcag ctccaactgt tccatcagga acgcggtgtc gcgcacagta tgctgctcgc
    44281 tggcgagatc aaaaccgtgt aaagaaagca aatattccgc gcgcattaac ggatgacgca
    44341 gcgtttgcca ggcctggtta atggttgcag attgctgtac ggcggcgagt tgttccgcct
    44401 ggcttccgct ggcgaattta tcaggatgat actgacgttg tagatcctga aaacgcaggc
    44461 tcagcgcctg ggtatcgagt tgataacggg caggcaagcc aaagaaggtg aagtaatcca
    44521 taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa caaaccccac
    44581 gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc tttcgccgca
    44641 accacactca tctttgacgt tcgggttggt gaatttaaac ccttcgttca ggccttcttt
    44701 tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt
    44761 cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac
    44821 ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa accctttgcc
    44881 gcggttagcc agaaaggtat ttactcgcgc tgctgcactg tcgctcagtg taatcgacat
    44941 aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca atggcggctt
    45001 tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt tcaagttctt
    45061 cagcaatatc ggtatttttg atcgcctgcg cttcgtcgag agacttccct ttcacccatt
    45121 cggtgaccag ggagctggaa gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt
    45181 cttcaatgat accttcatcg ttgactttaa tctgcaactt catcacgtcg ccacaggccg
    45241 gtgcccccac catgccgctg ccgacgttct cgtcgttgtt gtcaaaggaa cccacgttac
    45301 gcggattctc gtaatggtcg ataacttttt cgctgtaagc cattataaat tctcctgatt
    45361 ccgataccga ttaatgatga gcccattcga tgctgttcag atccacgccc tgcttgtaca
    45421 tttcccacag cggagaaagg tcacgcagac gaccgatgga tttacgaact aactcgatgg
    45481 tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata gagctatgtg
    45541 ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg cttgctgacg
    45601 tacaggcgga acctgaagaa actgcgaggt ctttcagcgc cataatcagc gactcacctt
    45661 caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc gtgttccagg tcaccgttca
    45721 ggtaaacttc ttcgatatct ttgatgccgt tccacagacg gttacgcagg ccgcgcagac
    45781 gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcctcgccc attccgacga
    45841 tctggtgaac aggcagagtg ccggaacgca taccgcgctc gtgaccgccg ccgtgcattt
    45901 gcgcttcgat gcgtacgcgc ggtttacgac gtacatacag cgcaccgata cctttcgggc
    45961 catagatttt gtgaccggag aaagacatca ggtcaacttt caactggctc aggtcgatag
    46021 gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat aatgccacga gcacggcaca
    46081 tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg tgcatgatgg
    46141 aaacaaggat ggtgtcatca cgcatcgctg cttcaagttc tttcagatcg ataatgccgt
    46201 tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg ctccagctga cggcaggtat
    46261 ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt ttctgataaa
    46321 agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag gtaaagacga
    46381 tttcacgcgg atcagcgccg accagatcgg caatctgatt acgggcgata tctaccgctt
    46441 cttcagcctg ccagccgaaa cggtgagaac gggaggccgg gttaccaaag gttccgtcca
    46501 tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt
    46561 agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact caatgcaagg
    46621 aaccaggcta ccggctggat gtacgaccgt gtttacggag cgcgatacgc cccggcctga
    46681 ttctaatatc ttgttatctt taacctaact gtttcgggtt tcagcaagtt gacaaactta
    46741 cgaatctccg ggagcttaca taagtaagtg accggagtga gtatgtgcag tcaacgcaga
    46801 tgcaacacga aacaggacgg ttaaattaag cgcgtaactt aacgtcgatc gcgtcttgta
    46861 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
    46921 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
    46981 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcaaccgcct ttaccctgac
    47041 aacgggtagc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
    47101 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
    47161 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
    47221 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
    47281 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
    47341 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
    47401 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
    47461 ccattttttt atcgtggcgt ttgtacctgt tgtccttcca gaccatgggc gggttgaggg
    47521 caattattcg cattagcgct ggcctcttcc cctcacccta accctctccc cgaaggggcg
    47581 aggggaccgt ccgtgcattt atctggctta gccctcaggc cttattacct ttattctgct
    47641 gctctataga agccagaatc ccgcgcagga tattcaattc ctggctttcc gggcgcgcac
    47701 gggtaaacag acggcgcaat ttattcatca cctgccccgg atggttttca cggataaaac
    47761 cggttgccag cagcgtttgt tccagatgac cataaaaacg ctccagatca tcgaccagcg
    47821 gatacggcgt ctcttcatgc tcgacctgct cgccattttc ctgagttgcc agccaggcca
    47881 tacgcacttc ataggcaata acctgaaccg ccattgccaa gttcagcgaa ctgtattccg
    47941 ggtttgccgc aatcgcgaca tgataatggc atttctgcaa ctcttcattg gtcaagccga
    48001 cgcgctcacg accaaacacc agcgccaccg gggtatttgc cgcttcagcg acgcttttca
    48061 gaccgcattc gcgcgggtcg agcatcggcc acggcagcgt gcgggagcgt gcgctggtgc
    48121 ccaccaccag gctacaaccg gctaacgctt cgtcgagcgt atcgacgatg tgagcattac
    48181 caatcacatc gctggcccct gctgccaggg caatcgcctg ggagtcgggt ttcaccagtg
    48241 gattaaccag ccacagattg gttaatccca ttgttttcat ggcacgggca acagaaccca
    48301 tattgccggt gtgtgacgtc tccaccagca caattcgaat attttgcagc attgtctttc
    48361 ttcatctaaa gattattcac gcatcttatc ataaaacgaa gacagatgcc gatctcgctg
    48421 ctatactctg cgccgttttc ccgttcttta acatccagtg agagagaccg atgcatccga
    48481 tgctgaacat cgccgtgcgc gcagcgcgca aggcgggtaa tttaattgcc aaaaactatg
    48541 aaaccccgga cgctgtagaa gcgagccaga aaggcagtaa cgatttcgtg accaacgtag
    48601 ataaagctgc cgaagcggtg attatcgaca cgattcgtaa atcttactca cagcacacca
    48661 tcatcaccga agaaagcggt gaacttgaag gtactgatca ggatgttcaa tgggttatcg
    48721 atccactgga tggcactacc aactttatca aacgtctgcc gcacttcgcg gtatctatcg
    48781 ccgttcgtat caaaggccgc accgaagttg ctgtggtata cgatcctatg cgtaacgaac
    48841 tgttcaccgc cactcgcggt cagggcgcac agctgaacgg ctaccgtctg cgcggtagca
    48901 ccgctcgcga tctcgacggt accattctgg cgaccggctt cccgttcaaa gcaaaacagt
    48961 acgccactac ctacatcaac atcgtcggca aactgttcaa cgaatgtgca gacttccgtc
    49021 gtaccggttc tgcggcgctg gatctggctt acgtcgctgc gggtcgtgtt gacggtttct
    49081 ttgaaatcgg tctgcgtccg tgggatttcg cggcaggcga gctgctggtt cgtgaagcag
    49141 gcggcatcgt cagcgacttc accggtggtc ataactacat gctgaccggt aacatcgttg
    49201 ctggtaaccc gcgcgttgtg aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg
    49261 ctctgaagcg ttaatgactc aggcgggtga tgtcactcac ccgccctcgc ctttcaggcg
    49321 ctattctgaa atacttcctc accgctttac tttctttcgt cactctccca ccatctttcc
    49381 ccgattaatg gatgataaag aagtcacaag gaaaggttat ggcgctgcca gtgaacaaac
    49441 gcgttctcaa aattctgttt attctctttg ttgttgcctt ctgcgtttat ttagtgccgc
    49501 gcgttgccat caacttcttc tattatcctg acgataaaat ttacggtccc gacccctggt
    49561 cggcggaatc cgtcgaattt acggctaagg acggtactcg tctgcaaggc tggtttattc
    49621 cttcttcgac gggccctgct gacaatgcca tcgcaaccat cattcatgct cacggcaatg
    49681 ccggaaatat gtccgcccac tggccgctgg tcagttggtt acccgagcgt aatttcaacg
    49741 tttttatgtt tgattatcgc gggtttggta aatcaaaagg cacgccgtcc caggccggat
    49801 tgctggacga tacgcaaagt gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac
    49861 aacggctggt gctgttcggg cagagtattg gcggggcgaa tattcttgct gttattggtc
    49921 aggatgatcg tgaaggcata cgtgcggtga tcctcgactc cacatttgcc tcttatgcaa
    49981 ccatcgccaa tcagatgatc cccggcagtg gctacttact tgatgagagt tacagcggcg
    50041 aaaattatat cgccagcgtc agcccgatcc cgcttttact cattcacggt aaagctgatc
    50101 acgttatccc atggcagcac agcgaaaagt tgtatagcct ggcaaaagag acaaaacggt
    50161 tgatcctaat cccggatggc gaacacattg atgctttttc cgatcgtcac ggcgatgttt
    50221 atcgcgaaca gatggtggac tttatcctta gcgcgttgaa tccgcagaac taacccatga
    50281 tcgctagcac gataatcatt cacaaaacca ccttaagaca tgctaatcca ctggtcagaa
    50341 cagtttaaga tgagaaaaat tctgtgacgc ttgccaacat ttctgatgat tagcattccc
    50401 ttcgccattt ccctgagcaa actttagcta ttcttatcaa ttatgcttat gggagatcta
    50461 cagatgatgc ctacgcttgc tccaccatct gtcctttcgg ctccccagcg ccgctgccag
    50521 atcttgctga cgctctttca gccggggttg accgccacca tggcaacctt cagcgagctt
    50581 aatggtgtgg atgatgatat tgccagtctt gatatcagcg aaacaggacg ggagatcctg
    50641 cgctatcatc aactcacact gacgactggt tatgacggta gctaccgggt tgaaggtaca
    50701 gtgcttaacc aacgtttgtg tttatttcac tggctacgac gtggtttccg tctgtgtccg
    50761 tcatttatta ccagccaatt cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt
    50821 gcgcgtaact tttacgacga taccaatcta caagcgttag tgaatctctg ctcccgacgg
    50881 ctgcaaaaac gctttgaaac gcgcgatatt catttcctgt gtctgtatct gcaatattgt
    50941 ttgctgcaac accacgctgg aattacgcct cagtttaatc cgctccaacg tcgctgggcg
    51001 gaatcctgcc ttgaatttca ggtagcgcag gaaattggac gccactggca gcgtcgggcg
    51061 ctccagcctg taccgcctga tgagccactg tttatggcat tacttttttc catgttgcgg
    51121 gttcccgatc cattgcgtga tgcgcatcag cgagacagac aattgcgtca gtctatcaaa
    51181 cgtctggtaa accattttcg tgagctggga aatgttcgtt tttatgatga acaggggtta
    51241 tgcgatcagc tttataccca cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt
    51301 attgataata ccctgccgga agagttcgcc agactgtacc cacgcctggt gcgcaccacc
    51361 cgcgcggcgc tggccggatt tgaaagtgaa tacggcgtcc atctttctga tgaggaaagc
    51421 ggtctggtcg cggtgatttt cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa
    51481 cagattattc tactgaccgg gaatgatagc gagcgagaag cgcagattga gcagcagtta
    51541 cgcgaactaa cgttactgcc gctcaacatt aagcatatgt cggtaaaggc atttttgcag
    51601 acaggcgctc cgcgtggcgc ggcactgatt attgcgcctt ataccatgcc attaccgctc
    51661 ttttcaccac cgctgatcta tacggacctg acgctgacaa cacatcaaca ggagcagatc
    51721 cgcaaaatgc tcgaatcagc atgagggaac aactttcggg cgcaaaaaca ttgccggaag
    51781 cgccaccagc gccattaccc agaacacgcc gtggcccaga tattgataca ggaaaccggc
    51841 gaaaacggtc atgatagcga tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa
    51901 acggatgact tcgctaccct ggcgaacagc aatatagcgc atggcggcca ggtggcagac
    51961 cgtgaaggtg ccgcaatgca aaatttgcac cactatcaac cacggcaacg ccgtagttgc
    52021 tcccataatg ccccagcgca ctacgccgca aatcgccgag atcaacagca tatcgcgagc
    52081 actacaacgg cggaaaagtt tattactcag cgcaaagata atgacttccg ccaccacgcc
    52141 cagcgaccac aaatacccca ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc
    52201 gctaaaaccg taataggccg catgtgcccc ctgcaataaa caaacgcagg ccagaaagcg
    52261 ccagttctgg cgaaccagcg ccaaccacgc agaccaaccg gtgctctcct gctggcgact
    52321 tgccccttgt ggctgaatcg tcggacggat gagaaagccg agcagcatgg atgccacgcc
    52381 caacgtcaac agcgcgagga tcacccgata atcaaacata gtgaccagtt tgcccgtcag
    52441 cgccgagcca atgacaaacg ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg
    52501 gaactgcttt tgccacgtat tcgccagtgc atcggtcaac ggtaccagcg gtgagaaaaa
    52561 gaggttaaag ccaatcatca ccagcatcag ccacgctacg tgcgcccccg cccagaaggc
    52621 gacagcaaag agaagtgtca gcagtgccag cacgcgcaag gcggaaatca ggcgggaagg
    52681 atcgctgacg cggggcgcga tgagcaaact cccgagaaaa cgggcaacca gacctgcccc
    52741 caataacagg ccgatggttt ctggcgttaa accaatccct ttaagccaga cgctccagaa
    52801 aggtagaaaa atgccgtaac taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt
    52861 ggattgcaaa accatgagtc cctcccgtca ggaggcgtta gtctggggtt aatcgaatgt
    52921 tggtgcaagt tgaaaaataa gaatattcga gcctgcttcc cgatgtctgt aacgccagtt
    52981 cacaagcact aaaaaactgc gatccgcgac gcatgttgca aaaataatgc gtaattatgc
    53041 cgttacgctt gccaaacgtt cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat
    53101 ctcccctttc ttccacgcca tcagtaaagc aatggaaggc acatttccgg caatgggacg
    53161 aaaaacaacc tgcccggtat taaaattatt catataaccg ggtatcaaag tgacgcccag
    53221 ccccatgccc accagattca tggtcaccag aatattcgtt gccacctgga cgatatttgg
    53281 ctggctgttt tcttgcgcaa accacgcttt aacgatcggc gcaagcgaac cggaatacgc
    53341 cggatcggta ctgacgaaat tcacgccatc cagttgcgcg gcggtgatct ctttttcatg
    53401 tgctaaaggg tgatcaaccg gtaacaccac cactaatggt tcgtcaaaaa gctccagata
    53461 atcaatctcc gggctataaa caggatggcg cattaagccg acgtcgagtt caccacggcg
    53521 aattttttcc tcctgttgcg tggtgattaa actcaccagc tcaatcaagg tgtctggctg
    53581 tctgagacga aacattggta atacttttgg caataaattc acttccgccg atggcacaaa
    53641 gccaatggtt aattgtctgt cttcctgaac aattttccgc gcccgtaatt tggcattttc
    53701 cgcttgttcg aggattgcca gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc
    53761 gactttgcgc ttatccctca ccagtaacgg aacaccgaca cagttttcaa gatcgcggat
    53821 ctggctgctt aacgaaggct gtgaggtatg cagtttttcc gccgcacggg taaagttcag
    53881 tgcctgcgcc actgcgacga aatagcgtaa atgccgtagt tccatcacct tccccttgtt
    53941 atcgaaaaaa cgtctcagct ggtagaaatt aactatttca catattagca accaaccaac
    54001 aacatcctta tgacacaaaa atagaaggtc aatacatctt atctttcagg attaaaaaat
    54061 atgaccacac cctcagattt aaacatttac caactgattg atacccaaaa tggtcgggtc
    54121 actccgcgta tttataccga cccagatatt taccaactgg agcttgaacg tattttcggc
    54181 cgttgctggt tatttctcgc ccacgaaagc cagatcccaa aacctggtga tttctttaac
    54241 acctacatgg gggaagatgc ggttgtcgta gtgcgtcaga aagacggcag cattaaggcg
    54301 tttctcaacc aatgccgcca ccgggccatg cgtgtgagtt atgcagattg cggcaacact
    54361 cgcgccttta cctgtccgta tcacggctgg tcttatggca ttaacggcga gttgatcgat
    54421 gtaccgctgg aacctcgcgc ctacccacaa gggttgtgta aatcccactg gggactaaac
    54481 gaagttcctt gtgtggagag ttataaaggg ctgatttttg gcaactggga taccagcgca
    54541 ccgggcctgc gtgattacct gggtgacatt gcctggtatc tggatggcat gctggatcgt
    54601 cgcgaaggcg gcaccgaaat tgtcggcggc gtacaaaagt gggtgatcaa ctgtaactgg
    54661 aaattcccgg cagagcagtt cgccagtgac cagtatcatg ctctgttcag ccatgcttct
    54721 gccgttcagg tattaggggc gaaagatgat ggcagcgata agcgcctcgg tgatggacaa
    54781 accgcccgcc cggtgtggga aaccgccaaa gatgcgctgc aatttggtca ggacggtcac
    54841 ggtagcggtt tcttctttac tgaaaaaccg gatgctaatg tctgggtcga tggcgcagtt
    54901 tcaagctatt accgcgaaac ctatgccgaa gcagaacaac gtttaggtga agttcgcgcc
    54961 ctgcgcctgg cgggtcataa caatattttc cccacgcttt catggctcaa cggcactgcc
    55021 acgctccgcg tctggcatcc gcgcggccct gatcaagttg aagtgtgggc gttctgtatt
    55081 actgacaaag ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct
    55141 tttggtcctg ctggttttct cgagcaggat gactcggaga actggtgtga aatccagaaa
    55201 ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag
    55261 gaaaagcgcc gcgacgacgg cattcctggc attactaact atatcttttc agaaactgcc
    55321 gctcgtggaa tgtaccaacg ctgggccgat cttctgagta gcgaaagctg gcaggaagtg
    55381 ctcgataaaa ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag
    55441 agttacatca ccgcattagc cagtttctct ttcacgaagc cagcttactg gacgactgga
    55501 aatttcgtga ctggctggag cagctcgaca aagagattcg ttacaccatg cgcaccacag
    55561 ttaacgcgca aacacgcgac cgccgcaaag gcgtccagcc accgacaacc tggattttta
    55621 atgacaccaa agaccagctg gagcggcgaa tcgcccgtct ggaaacgggc atggcctggg
    55681 cagaagagcc gccgtcacgc acccgtcact taatcagcaa ctgccagata agcgaaaccg
    55741 acatcccaaa cgtatttgct gtgcgggtaa attatctgct ttatcgggca caaaaagagc
    55801 gcgatgaaac attctatgtt ggaacgcgtt tcgacaaagt tcgccgtctg gaagatgaca
    55861 actggcgctt gctggaacgg gatatcgtcc tggatcaagc ggtaatcact tcccataacc
    55921 tgagtgtact gttctgatga atcgaattta tgcgtgtccc gttgcggatg tgccggaggg
    55981 tgaggctctc cggatcgata cctcgcccgt catcgccctg ttcaacgttg gcggcgagtt
    56041 ttatgccatt aacgatcgtt gcagccatgg taatgcgtcg atgtcagaag ggtatctgga
    56101 agatgacgcc acggtggagt gcccgctaca cgccgccagt ttttgcctga aaacggggaa
    56161 agcgttatgc ctgcccgcca ccgatccgct caccacttat ccagtacacg ttgaaggtgg
    56221 tgacattttc atcgacttac cggaggcgca gccatgagcg atctgcataa cgagtccatt
    56281 tttattaccg gcggcggatc gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa
    56341 ggcgcgcaag ttgccacgct ggaactgtcg gcggcaaaag tcgccagtct gcgtcagcga
    56401 tttggcgaac atattctggc ggtggaaggt aacgtgacct gttatgccga ttatcaacgc
    56461 gcggtcgatc agatcctgac tcgttccggc aagctggatt gttttatcgg caatgcaggc
    56521 atctgggatc acaatgcctc actggttaat actcccgcag agacgctcga aaccggcttc
    56581 cacgagctgt ttaacgtcaa cgtactcggt tacctgctgg gcgcaaaagc ctgcgctccg
    56641 gcgttaatcg ccagtgaagg cagcatgatt ttcacactgt caaatgccgc caggtatcct
    56701 ggcggcggtg gcccgctgta caccgccagt aaacatgccg caaccggact tattcgccaa
    56761 ctggcttatg aactggcacc gaaagtgcgg gtgaatggcg tcggcccgtg tggtatggcc
    56821 agcgacctgc gcggcccaca ggcgctcggg caaagtgaaa cctcgataat gcagtctctg
    56881 acgccggaga aaattgccgc cattttaccg ctgcaatttt tcccgcaacc ggcggatttt
    56941 acggggccgt atgtgatgtt ggcatcgcgg cgcaataatc gcgcattaag cggtgtgatg
    57001 atcaacgctg atgcgggttt agcgattcgc ggcattcgcc acgtagcggc tgggctggat
    57061 ctttaaggaa gcacgatgaa agaaaaaacg atcattattg tcggtggcgg gcaagcggcg
    57121 gcaatggctg cggcctcgct acgccagcaa gggttcaccg gtgagctgca tctgttttcc
    57181 gatgagcgac atcttcctta tgaacgacct ccactctcga aatccatgtt gctggaagat
    57241 tccccgcagt tacagcaggt gttacccgct aactggtggc aggaaaacaa tgttcatctg
    57301 cattccggtg taaccatcaa aacgctgggt cgcgacacac gagagttagt gttaaccaac
    57361 ggcgaaagct ggcactggga tcagcttttt atagcaatcg gcgcggcagc tcgaccgctg
    57421 ccgttgcttg atgcactggg agaacgctgc tttaccctac gccatgccgg tgatgccgcc
    57481 agactgcgag aagttctgca gcccgaacgg tcagtcgtga ttgtcggtgc cggaactatt
    57541 ggtctggaac tggctgccag cgccacgcag cgcagatgta aggtgacagt gattgaactg
    57601 gcggcaaccg tcatgggccg taatgcacca ccgcccgtgc aacgctatct tttacagcgc
    57661 caccagcagg ctggtgtgcg cattctgctc aataatgcca ttgaacatgt ggtcgatggt
    57721 gaaaaagtag aactgacgct gcaaagtggg gaaacgcttc aggctgatgt ggtgatttac
    57781 ggtattggta tcagcgccaa cgagcaactg gctcgcgagg ccaaccttga tactgccaat
    57841 ggcattgtca ttgatgaggc ttgccgcacc tgcgatcccg cgatctttgc cggtggcgat
    57901 gtggcaatca ctcgtcttga taatggtgca ctacaccgct gcgaaagctg ggaaaacgcc
    57961 aataaccagg cgcaaattgc cgctgccgca atgttagggc taccgctacc gctactgccg
    58021 ccgccgtggt tctggagcga tcagtacagt gataacttac agtttattgg cgatatgcgt
    58081 ggcgatgact ggctttgtcg tggcaacccg gaaactcaga aggcgatttg gtttaatctg
    58141 caaaacggcg tgcttatcgg tgcggtcacg ctgaatcagg ggcgtgagat tcgcccaatt
    58201 cgcaaatgga tccagagcgg caaaacgttt gatgcgaaac tgctgataga tgagaacatc
    58261 gcgcttaaat cactgtaacc aggataatta gcgaatatct caacgcctgg ggcgtggcga
    58321 ggtgcaagag tgtgtattac gtttaaatca cattatcttg caaagggatt ggttatgaac
    58381 acactac
//
LOCUS       DAZPJR010000007        49494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPJR010000007 DAZPJR010000000
VERSION     DAZPJR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.008"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            44..676
                     /gene="comR"
                     /locus_tag="ACHVB7_000432"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="ACHVB7_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR1912529.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(738..1277)
                     /locus_tag="ACHVB7_000433"
     CDS             complement(738..1277)
                     /locus_tag="ACHVB7_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1912530.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHVB7_000434"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHVB7_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR1912531.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3216..3758)
                     /gene="ycfP"
                     /locus_tag="ACHVB7_000435"
     CDS             complement(3216..3758)
                     /gene="ycfP"
                     /locus_tag="ACHVB7_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR1912532.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3781..4806)
                     /gene="nagZ"
                     /locus_tag="ACHVB7_000436"
     CDS             complement(3781..4806)
                     /gene="nagZ"
                     /locus_tag="ACHVB7_000436"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR1912533.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4817..5641)
                     /gene="thiK"
                     /locus_tag="ACHVB7_000437"
     CDS             complement(4817..5641)
                     /gene="thiK"
                     /locus_tag="ACHVB7_000437"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR1912534.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5622..6263)
                     /gene="lpoB"
                     /locus_tag="ACHVB7_000438"
     CDS             complement(5622..6263)
                     /gene="lpoB"
                     /locus_tag="ACHVB7_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR1912535.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVKAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6277..6654)
                     /locus_tag="ACHVB7_000439"
     CDS             complement(6277..6654)
                     /locus_tag="ACHVB7_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR1912536.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6657..7016)
                     /gene="hinT"
                     /locus_tag="ACHVB7_000440"
     CDS             complement(6657..7016)
                     /gene="hinT"
                     /locus_tag="ACHVB7_000440"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR1912537.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7350..9539
                     /gene="fhuE"
                     /locus_tag="ACHVB7_000441"
     CDS             7350..9539
                     /gene="fhuE"
                     /locus_tag="ACHVB7_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR1912538.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(9599..11032)
                     /gene="ptsG"
                     /locus_tag="ACHVB7_000442"
     CDS             complement(9599..11032)
                     /gene="ptsG"
                     /locus_tag="ACHVB7_000442"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR1912539.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11327..12124)
                     /locus_tag="ACHVB7_000443"
     CDS             complement(11327..12124)
                     /locus_tag="ACHVB7_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1912540.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12135..13139)
                     /gene="holB"
                     /locus_tag="ACHVB7_000444"
     CDS             complement(12135..13139)
                     /gene="holB"
                     /locus_tag="ACHVB7_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR1912541.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13136..13777)
                     /gene="tmk"
                     /locus_tag="ACHVB7_000445"
     CDS             complement(13136..13777)
                     /gene="tmk"
                     /locus_tag="ACHVB7_000445"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1912542.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13767..14789)
                     /gene="yceG"
                     /locus_tag="ACHVB7_000446"
     CDS             complement(13767..14789)
                     /gene="yceG"
                     /locus_tag="ACHVB7_000446"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR1912543.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14792..15601)
                     /gene="pabC"
                     /locus_tag="ACHVB7_000447"
     CDS             complement(14792..15601)
                     /gene="pabC"
                     /locus_tag="ACHVB7_000447"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR1912544.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15721..16962)
                     /gene="fabF"
                     /locus_tag="ACHVB7_000448"
     CDS             complement(15721..16962)
                     /gene="fabF"
                     /locus_tag="ACHVB7_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR1912545.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17050..17286)
                     /gene="acpP"
                     /locus_tag="ACHVB7_000449"
     CDS             complement(17050..17286)
                     /gene="acpP"
                     /locus_tag="ACHVB7_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1912546.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17497..18231)
                     /gene="fabG"
                     /locus_tag="ACHVB7_000450"
     CDS             complement(17497..18231)
                     /gene="fabG"
                     /locus_tag="ACHVB7_000450"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR1912547.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18244..19173)
                     /gene="fabD"
                     /locus_tag="ACHVB7_000451"
     CDS             complement(18244..19173)
                     /gene="fabD"
                     /locus_tag="ACHVB7_000451"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR1912548.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19189..20142)
                     /gene="fabH"
                     /locus_tag="ACHVB7_000452"
     CDS             complement(19189..20142)
                     /gene="fabH"
                     /locus_tag="ACHVB7_000452"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR1912549.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20210..21280)
                     /gene="plsX"
                     /locus_tag="ACHVB7_000453"
     CDS             complement(20210..21280)
                     /gene="plsX"
                     /locus_tag="ACHVB7_000453"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR1912550.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21361..21534)
                     /gene="rpmF"
                     /locus_tag="ACHVB7_000454"
     CDS             complement(21361..21534)
                     /gene="rpmF"
                     /locus_tag="ACHVB7_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR1912551.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21586..22107)
                     /gene="yceD"
                     /locus_tag="ACHVB7_000455"
     CDS             complement(21586..22107)
                     /gene="yceD"
                     /locus_tag="ACHVB7_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR1912552.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22306..22890
                     /gene="yceF"
                     /locus_tag="ACHVB7_000456"
     CDS             22306..22890
                     /gene="yceF"
                     /locus_tag="ACHVB7_000456"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR1912553.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23002..23961)
                     /gene="rluC"
                     /locus_tag="ACHVB7_000457"
     CDS             complement(23002..23961)
                     /gene="rluC"
                     /locus_tag="ACHVB7_000457"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR1912554.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24534..27719
                     /gene="rne"
                     /locus_tag="ACHVB7_000458"
     CDS             24534..27719
                     /gene="rne"
                     /locus_tag="ACHVB7_000458"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR1912555.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27914..28867)
                     /gene="flgL"
                     /locus_tag="ACHVB7_000459"
     CDS             complement(27914..28867)
                     /gene="flgL"
                     /locus_tag="ACHVB7_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR1912556.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28879..30522)
                     /gene="flgK"
                     /locus_tag="ACHVB7_000460"
     CDS             complement(28879..30522)
                     /gene="flgK"
                     /locus_tag="ACHVB7_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR1912557.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30588..31529)
                     /gene="flgJ"
                     /locus_tag="ACHVB7_000461"
     CDS             complement(30588..31529)
                     /gene="flgJ"
                     /locus_tag="ACHVB7_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR1912558.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31529..32626)
                     /gene="flgI"
                     /locus_tag="ACHVB7_000462"
     CDS             complement(31529..32626)
                     /gene="flgI"
                     /locus_tag="ACHVB7_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR1912559.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32638..33336)
                     /gene="flgH"
                     /locus_tag="ACHVB7_000463"
     CDS             complement(32638..33336)
                     /gene="flgH"
                     /locus_tag="ACHVB7_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR1912560.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33389..34161)
                     /gene="flgG"
                     /locus_tag="ACHVB7_000464"
                     /pseudo
     CDS             complement(33389..34161)
                     /gene="flgG"
                     /locus_tag="ACHVB7_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(34333..35088)
                     /gene="flgF"
                     /locus_tag="ACHVB7_000465"
     CDS             complement(34333..35088)
                     /gene="flgF"
                     /locus_tag="ACHVB7_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR1912561.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35108..36313)
                     /gene="flgE"
                     /locus_tag="ACHVB7_000466"
     CDS             complement(35108..36313)
                     /gene="flgE"
                     /locus_tag="ACHVB7_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR1912562.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36338..37033)
                     /gene="flgD"
                     /locus_tag="ACHVB7_000467"
     CDS             complement(36338..37033)
                     /gene="flgD"
                     /locus_tag="ACHVB7_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR1912563.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37045..37449)
                     /gene="flgC"
                     /locus_tag="ACHVB7_000468"
     CDS             complement(37045..37449)
                     /gene="flgC"
                     /locus_tag="ACHVB7_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR1912564.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37453..37869)
                     /gene="flgB"
                     /locus_tag="ACHVB7_000469"
     CDS             complement(37453..37869)
                     /gene="flgB"
                     /locus_tag="ACHVB7_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR1912565.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38024..38683
                     /gene="flgA"
                     /locus_tag="ACHVB7_000470"
     CDS             38024..38683
                     /gene="flgA"
                     /locus_tag="ACHVB7_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR1912566.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            38759..39052
                     /gene="flgM"
                     /locus_tag="ACHVB7_000471"
     CDS             38759..39052
                     /gene="flgM"
                     /locus_tag="ACHVB7_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR1912567.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            39057..39473
                     /gene="flgN"
                     /locus_tag="ACHVB7_000472"
     CDS             39057..39473
                     /gene="flgN"
                     /locus_tag="ACHVB7_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR1912568.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39513..41048)
                     /gene="murJ"
                     /locus_tag="ACHVB7_000473"
     CDS             complement(39513..41048)
                     /gene="murJ"
                     /locus_tag="ACHVB7_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR1912569.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41158..42081)
                     /locus_tag="ACHVB7_000474"
     CDS             complement(41158..42081)
                     /locus_tag="ACHVB7_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1912570.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42083..42730)
                     /locus_tag="ACHVB7_000475"
     CDS             complement(42083..42730)
                     /locus_tag="ACHVB7_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR1912571.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42741..43325)
                     /gene="rimJ"
                     /locus_tag="ACHVB7_000476"
     CDS             complement(42741..43325)
                     /gene="rimJ"
                     /locus_tag="ACHVB7_000476"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1912572.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43561..44769
                     /gene="mdtH"
                     /locus_tag="ACHVB7_000477"
     CDS             43561..44769
                     /gene="mdtH"
                     /locus_tag="ACHVB7_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR1912573.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            44833..45480
                     /gene="grxB"
                     /locus_tag="ACHVB7_000478"
     CDS             44833..45480
                     /gene="grxB"
                     /locus_tag="ACHVB7_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR1912574.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45614..46174
                     /locus_tag="ACHVB7_000479"
     CDS             45614..46174
                     /locus_tag="ACHVB7_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1912575.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46280..47326
                     /gene="pyrC"
                     /locus_tag="ACHVB7_000480"
     CDS             46280..47326
                     /gene="pyrC"
                     /locus_tag="ACHVB7_000480"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR1912576.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47400..47645
                     /gene="dinI"
                     /locus_tag="ACHVB7_000481"
     CDS             47400..47645
                     /gene="dinI"
                     /locus_tag="ACHVB7_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR1912577.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47935..48189
                     /gene="bssS"
                     /locus_tag="ACHVB7_000482"
     CDS             47935..48189
                     /gene="bssS"
                     /locus_tag="ACHVB7_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR1912578.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            48304..49422
                     /gene="solA"
                     /locus_tag="ACHVB7_000483"
     CDS             48304..49422
                     /gene="solA"
                     /locus_tag="ACHVB7_000483"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR1912579.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        12022 a        13338 c        12279 g        11855 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gttcaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactgatgca gattgccgga acgactttgc gtttatggcc
      661 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
      721 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt actatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc accaaactga
     1021 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccccg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
     1381 ggattcggag aagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1621 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccgaca gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
     1741 atttttcagc ggtttaccgt tcatctgcgc cagaatattt ttcattgcgc aggtcgccat
     1801 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttgg tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccacacca tcagatcagc ctcaatatat tcaccatctt tagtgtgcag
     2041 gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg accgcattgt acaattcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt taccattcgc gcccaggttg gcggagtatt tcaggaacag
     2341 attcagcatc tcctggtgga agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
     2461 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gcggtttcca cagatggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatttt tttcaatggc gtagtcaacg tgaccccctt
     2821 aaatttatta accaattgtt aattaatggt tataaacata gctcttaatt aacaacaggt
     2881 tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc
     2941 aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc gggtgagatc
     3001 aaaaaacaga gtttattgtt tattatttga gcaagtccgg aagggtgata tagggtgacg
     3061 cgtatatcga gggcgtttgg tcgtcctgat aagatgcgca gcatcgcatc aggcattgat
     3121 gctcgactgt cggatgcggc gtaaacgcct tatccgactt acagaattcg gagcgcatca
     3181 ggtaccggca aggttacctg atgcgacgag ggcatttacc cgagggtttt gaacgcctta
     3241 atgcgctgca aatgcgggga gatattcttg aatttgtgcg tctgctcttc gtcccagaca
     3301 atctcgtaat aatgatgcaa ctcttcagat gtccgctggc tgttaagcgc ttcatcatta
     3361 cgcgacaaaa tcaccaggca acgatcgcga ttcttctcgc ggaagttggt cacacactta
     3421 gtggcaatat cggcatactc ttccgggcga tcaatcttgc cttccatgtt ctcataaggg
     3481 aacaaattag ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat ccgttccgcc
     3541 cagtatccgc ccagaccaac gccgcaaatc agcggacgct cgtcgacgtt cagttgcaac
     3601 attttgtcca cttctttgag cagatgctgc atatcatgtt tcggatgccg cgtactgtag
     3661 cttatcaagc gtacatccgg gtcaataaac tgcaattgta agactttctc gtggttaccc
     3721 ggactgttag agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca taagccaggg
     3781 ttagtgacct gctttctctt cctgccagcg ttcatgtaac tgattcagac gggtgctgat
     3841 cgccttccag cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt tatgatacaa
     3901 acgtgtaaca cgttctgcct tgatcggtga cagattatct aacacgctga cggccccttt
     3961 acgattattg cagaccagga tcatatcgca acctgcgtcc agtgatgcct gcccgcgttc
     4021 ggcataactg cccataatcg cggcaccttc catcgataaa tcgtcagaga aaattacacc
     4081 gtcaaaacac agttcctgac gcaaaacggt tttcagccag taggaagaac cgctcgccgg
     4141 acgcggatca acatcactgt agatcacatg cgcaggcata atggcgtcga gtttattttc
     4201 gcggattaag gaactgaaga ccgacatatc tttagcgcga atctccgctt gtggacgtgg
     4261 atcgcacggt gtttctttgt gtgagtctgc cgttactgca ccgtgtcctg ggaagtgttt
     4321 cccggtcgtt ttcattccgg cttcatgcat accatcaata aaccggctgg caattgccag
     4381 ggctttttgt ggatcggcat gataagaacg ctcgccaatc gccgcgctga tatgcccgac
     4441 atccagcact ggcgcaaagc tgatatcaat atccatagcg atcatttcgc tggccatcag
     4501 ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt cccaacagcg cagcaaagga
     4561 ttgtgctgcc ggtaagcggg taaaaccttc gcgaaaacgc tgcacgcgtc caccttcctg
     4621 atctaccgcc accaccagat gattgcgcga tgctgcgcgg atctggcgca ccagttcacg
     4681 taactgggca ggatcatgat agttacgcgt aaagagaatc agccctccca ccagcggatg
     4741 cgccagtatt tcacgctctt ccgcgtccag ttcgtaacct tcgacatcca acattactgg
     4801 acccacactg ctctccttat tgttttatta atagctgccg ccaggtgtca tcggccagcc
     4861 tgataaattg ttgatcgccg atttgtcgcc agcggtactc aaaccaccct gctttgagca
     4921 tcagcagcca gggaaaccat cgcctgacct gacgccataa ttgcgccgga taaatcttcg
     4981 cgcgagtggc atagtcattg accaattgcc ggtgctgttc agtattttcc acccacaccg
     5041 ccgccagttc cagcgcgata tcaccatctc cggcatactc ccagtcgatg agttttaacc
     5101 ctgacgcgct atgcactaaa tttccggcgt ggacatccat atgcaatgga cttaagcgta
     5161 aaggccgtgg ttcccgcgct ttgcgcagac gttttaacat tcgcagccaa cccacagtcc
     5221 gccgcgccgg atcgctttgc tgccagtaca gttccagtaa gggcaacagc gttattcgcc
     5281 agccaaaacg tggttgttga tgaagataat acagcaagcc tgccagttcg ttggtatccg
     5341 gcaaatacgt ttttaccgcg ccgggcagat agtcgactac catccagtca cggagatata
     5401 aatgcggctt cggtgcaatg cttgcgggta gttgtgataa agcccgatac tggcgcaaga
     5461 acgcggactg cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc tggccctgat
     5521 gttcaatgag aaaactaccg ccactaagcc cactattaaa cgtcgtgacg ggatgaaact
     5581 gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg attattgctg cgaaacggca
     5641 cctttacctg accagataat ttcgcccgtc tgcaccagca tcagctgcat ttgtagggtc
     5701 ggagccttaa cgttgccaga ggcgctggag tacagcacgt aatgagcgcc gacattgcgg
     5761 gcaatgccta tggctttact acgggtacct aaactgtcct gcggcgacaa acctaactgt
     5821 tgcttagcca tcgacagctg ctgggcggaa accagggtaa atttcccgtt attagccagc
     5881 gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt acggttgtta
     5941 acgctatcaa ccagcaggac gctacccgca gtcaccccgt cagccccaag catcttactg
     6001 accatcggct gcattgcgcc attccagtca taatggcgaa tatgcggcgc aggcggtgcc
     6061 gtttgatctt cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac cgagggcact
     6121 gtggggacag gctgttgtgg ctcggctggt tgttccggcg ctggtttcac ttcttctacc
     6181 ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca tcgccagcgc ggtaatcaag
     6241 gcgtagcgac tcatttttgt catcaagatt caccccttac aaatatagat aaagtctgac
     6301 tttgtgcgcc cccagaaaat tggcgctgcc gtacagcgtt accgccgaat gtgcgggaat
     6361 gctaacgctg cgtggccttt ccagaggatg catctccagc cctctggcgt cataccagta
     6421 aaaacgataa tgaacggtaa cgggttcttg cctttcgtta taaagcgttg aggatgctga
     6481 aggttgaata tcagacgtcg aaaggacggg cttttctgca ctgatgcccg cagccaataa
     6541 agatgactcc atcaccagcg attgctcatc attcaccgga atttccggat gtgaacgaca
     6601 gcccaccagc agtaacaacg ccagagacac cagcccaaag catccttttc tcatcgttac
     6661 agacctttat gcgccagcat tggtcccagc ggacggccac ccaacaagtg catatggatg
     6721 tggtaaacct cttgtccgcc atggcggttg gtattcatga tcagacgata gccatcttcg
     6781 gcaatacctt cttgctcagc aatttttgcc gctacggtga tcatgcgtcc cagcgcctgc
     6841 tcatgctcag ctgacacgtc gttcacagtc ggaatgagga tattcggaat gatcagaata
     6901 tgcgttggcg cttgcggcga aatatcgcga aacgccgtta ccagatcatc ctggtagacg
     6961 atatcggagg ggatctcacg acgaataatt ttgctgaata tagtttcttc tgccacgaca
     7021 ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat
     7081 agagtatggt cgagttaaac ctgctctttc aaccttaact cacgatttct taagcaaaaa
     7141 atcgttttgc tgccgtatat atcgccatta ttcccatttc tgcctgcgaa tgcgctgaaa
     7201 agacattctc tgtttggttt acacgacaaa tatgaatgcg catatttctc atttgcattt
     7261 acaaacaaaa ttattcgcac actaaatata accgctcatc accctcctcc ggcatgagcc
     7321 tgacgacaac ataaaccaag agatttcaaa tgctttcaac acaatttaac agggataatc
     7381 aatatcaagc catcaccaaa ccgtcactac ttgccggttg catagcactg gcactattac
     7441 cttctgccgc ttttgctgca ccagccactg aagaaacggt gattgttgag ggttcagcca
     7501 cagctccaga tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg ggtaccaaaa
     7561 tgcagatgac tcaacttgat attcctcagt cggtcactat tgttagccag cagcggatgg
     7621 aagatcagca gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg atcagcaaaa
     7681 gtcaggcgga ttccgatcgt gctctttatt attcccgtgg attccagatc gataactata
     7741 tggttgatgg tatccccacc tattttgaat cgcgctggaa tctgggcgac gcactttctg
     7801 atatggcact ctttgaacgc gtagaagtag tgcgtggcgc gacaggactc atgaccggga
     7861 cgggtaatcc atctgcggca attaatatgg ttcgaaaaca cgcgaccagt cgtgaattta
     7921 aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga acggtatgtg gcggatttac
     7981 aaagcccact caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc taccagaata
     8041 acgactcatg gctggaccgc tacaacagtg aaaagacctt cttctcgggc attgtcgatg
     8101 ctgatttagg cgatcttacg acgctttcag ccggttacga atatcagcgc attgatgtta
     8161 atagcccaac ctggggcggt ttaccgcgct ggaatactga tggcagcagc aacagttacg
     8221 atcgtgcacg cagtaccgca cctgactggg cgtacaacga taaagagatc aacaaggtct
     8281 ttatgaccct gaagcagcgg tttgctgata cctggcaagc gacactgaat gccacccact
     8341 ctgaagtcga atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac aaagcggatg
     8401 gtatgctggt tgggccatac agtaattatg gacctggctt tgattatgtc ggcggcaccg
     8461 gttggaacag tggcaaacgt aaagttgatg cgctggattt gttcgctgac ggtagttatg
     8521 aattgtttgg tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa caaaacaatc
     8581 gttacttcag ttcatgggcc aacatcttcc cggatgaaat tggcagtttc tacaacttta
     8641 atggcaattt cccacaaacc gactggtcac cacagagcct ggcacaggac gataccacac
     8701 atatgaaatc gttatatgct gctactcgag tcacccttgc cgatccgctg catctgatcc
     8761 tcggcgcacg ttataccaac tggcgggttg atacgctgac ttacagcatg gagaaaaacc
     8821 acaccacgcc ttacgctggt ctggtgtttg acatcaatga caactggtcg acctacgcca
     8881 gctatacctc tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa tatctggctc
     8941 caatcaccgg taacaactac gagctgggtc tgaaatcgga ctggatgaat agccgtctga
     9001 ccaccacgtt agccatcttc cgtattgagc aggataatgt cgctcagtcc accggtacac
     9061 ctatccccgg cagcaacggc gaaaccgcct ataaagcggt ggatgggaca gtcagtaaag
     9121 gggtggaatt tgaactcaac ggcgcaatta ccgacaactg gcagctgaca tttggcgcaa
     9181 cgcgctatat tgcagaggat aacgaaggaa acgccgttaa tcctaatctg ccacgcacca
     9241 cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc agagttgaca gtcggcggtg
     9301 gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac accgtatggc accttccgcg
     9361 ccgagcaagg tagctacgcg ctggtggatc tcttcacccg ctaccaggtg acgaaaaact
     9421 tctcgttaca ggggaacgtc aataacctgt tcgacaaaac ctacgatacc aacatggaag
     9481 gttctatcgt ctacggcgca ccgcgtaatt tcagcattac cggcacgtat caattctgaa
     9541 taacacctgt aaaaaaggca gccatctggc ggccttagtc tccccaacgt cttacggatt
     9601 agtggttacg gatgtactca tccatctcgg ttttcaggtt atcggattta gtaccgaaaa
     9661 tcgcctgaac accagaacca gcaaccacta cgcccgctgc gcccagtttc ttcaggccag
     9721 cctgatccac tttagacaca tcagcaacgc tgacgcgcag acgggtaata catgcgtcga
     9781 ggttagtaat gttttcttta ccaccaaatg cagcaaccag agccggtgcc atttcgctgg
     9841 tacctgttgc ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc agatccagtg
     9901 ctttaatcag cacgcggaag atggtgtagt aaacaatcgc ataaccgata ccgacgatcg
     9961 ggaacagcca cagtttgctg ctgttaccag acagaacgat aaagtcgatc agaccgtgcg
    10021 agaacgacgt accgtcacgc atccccagaa gaatacagat tgggaatgcc aggcctgcca
    10081 gaatcgcgtg gatgatgtac aggatcggcg caacgaacat gaaggagaac tcgatcggct
    10141 cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat cataataccg cccactttcg
    10201 cgcggttttc tggtttagca gagtgccaga tagcaattgc ggcagctggc agaccgtaca
    10261 ttttgaacag gaagccacca gacagtttac ccgcagtcgg gtcacccgcc atataacgcg
    10321 gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc tgcatctgga
    10381 aaggtacgtt ccagatgtgg tgcagaccaa acggcaccag gcaacgttcg atgaaaccgt
    10441 aaatgccaaa cgcaactacc gggttctggt aagcagccca ctgagagaag gtctggattg
    10501 cagaaccaat cggcggccaa atgaaggaca gcacaacgcc agtaaagatg gcagccaggc
    10561 cagaaatgat cggcacaaag cgtttaccgg caaagaagcc aagatactca ggcagcttaa
    10621 tacggtagaa acggttaaac atgtacgctg cgatcgcacc ggagataatc cctccgagta
    10681 cgccagtatc cgccaggtgt ttagaggcga tttcttcagc aggtaaatgc agtaccagtg
    10741 gcgcaaccac ggccatggtt ttaaccatga tgccataggc aacaactgcg gccagcgcgg
    10801 atacgccatc gttattggta aagccgaggg cgacaccgat cgcaaaaatc agtggcatgt
    10861 ttgcaaagac ggaaccgcct gcttctgcca taacatgcga tacaacggcg ggcagccagc
    10921 tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat aggcagtacg gataccggca
    10981 gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc attcttaaac ataattgaga
    11041 gtgctcctga gtatgggtgc tttttttacg ttctcacgcg tggcaagggg ggagagcctc
    11101 gccgtgtaca gggcatctaa gcgcccttta tttattacac agagtaaaat aattcagtgc
    11161 cgatttgttt gacggctatc acatttcaac ttcagatcat tggagaagtt gcacattttg
    11221 acaaaacata tgtcacaata tttcaaacgt ggttattcac cgcccaattt tcggcggtga
    11281 actttactcg ttttagccat taattacgag ctttaaaaaa actcattcaa cggatggatt
    11341 gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt acctgcgcca
    11401 gttcttcaac ggcaacacct ttcaacacag ccatgtattc tgcaacgtca cgaaccatcg
    11461 caggttgatt ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag tcagtttcca
    11521 ccagtaaccg atccaggggg acataacgcg cagcatctcg aagttgctcc gcattacgga
    11581 aggtcacaat gccggaaaag gagatgtaaa atccgagatc cagtaattta cccgccgttt
    11641 ctctgtcctc tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt tcttcgcgca
    11701 gaattgccag cgtatcggcg cgggcgtcac gggtatggac gataaccggc ttgttcagtt
    11761 cacgaccaat ctggatatga tggatgaagg actcttgctg acgaacttta gtttccggtg
    11821 tgtaataata atccagcccg gtttcaccca gcgctacaac accctcttct gccgccagac
    11881 ggcgtaaatc ttctacatcg tagggatcat tctggttgag tggatgcacg ccacaagaaa
    11941 atacgacgtt gtcacgttcg cctaccagat cccgcatatg taaataaccc ggtaatgttg
    12001 tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga acgtcatcca
    12061 cgtccttatg caaagattca taatccagac catcgagatg gcagtgtgag tcgactaaaa
    12121 acatgatgtc tctcttaaag atgaggaacc ggtagcacaa cgcccggttg caggtaatgc
    12181 tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt gacagacatt
    12241 aactgttcac gaatgtggca aacatccccc agtatagcct gcaggcgcga gggagaaaga
    12301 tggtttgcca gttcgacgac aaggcccggc acatcaacat tggtcacctg cgcagcacca
    12361 tgatggcgtt ttagcgcatc catcagcaac gttgccagcc agtgtaaacg cgccggagct
    12421 tgttcatgat taagggccgc cagcagcgaa taccagtcgc ccgatggcac gctatatgcc
    12481 aatgcctgac acaatgtttc acgagcctgc cagttatctc cctgaaacaa cgccagtgcc
    12541 gcgccaggcg aaccggcgct taagcgcaat gcggcaagta atgcatcctg tgacattgtc
    12601 acttcgcgtg aaagccaggt cacggcgtac tgttccggcg gcggcgcaag gtaatgtaac
    12661 cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc caggaaaaac
    12721 caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc ggcgtcggtc
    12781 agtaaggcag catcggttac ccagacaact ttcgcaccac ctaggcgtgc gtgctcattc
    12841 agcttttcgg tgacctcacg tactgcatca atgcccagcg tatttttccc tttttcggga
    12901 gccagggtgt agtaatcggg atgcgtgcca gcctgcatca actgacatcc acgacagtga
    12961 ccgcaacttt tgtggccctg cggttgttgg cagagtaaat aacggctcag ggcgtagatt
    13021 aaagcatcat cgcccatgcc cggtaacgcc tgaatgagta gcgcatggtg acctcttccg
    13081 gcctgatagc tggctaccag tttttcgaaa tcaggtcgta accatggata ccatctcatg
    13141 cgtccaactc cttcacccag tgggtcacgg tagtgcggat tgcatccatc acggcctcca
    13201 gcggctgggt ggcatcaatg gtatgaatac ttttatcttg tgcagccagt tccagatagc
    13261 gggcgcgggt gcgattaaag aaatcgaaag actcttgctc aatacgatcc agctcgccgc
    13321 gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc gagatagagc gttaagtcgg
    13381 ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag catatgttgg tcaataccac
    13441 gtcctccgcc ctgatacgcc tgagtggaga gatcgtggcg atcgccaatc acccaggtgc
    13501 cgttagccag agctggtttg atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca
    13561 tcagaacttc ggctttatcg gtaatgactt catcgcctac cgatttgata tccagcacca
    13621 ggcttcttaa cttttcggca agttgcgtac cgccaggttc ccgagtgaaa accatgtcgc
    13681 ggatacccag ttgctcaagc gtctcaacca ccacattacg cgcggtagtt ttgcctgcgc
    13741 cttccagccc ctcaatgacg atatacttac tgcgcatttt tttccttaag cactttcaga
    13801 taatcctgca cagacttgtt atggctggca agattggtat taaacgtgtg accaccttta
    13861 ccatcggcca caaaatagag atacggcgtt tttgccggat gcgcagcagc cttcagcgaa
    13921 tccgcccccg gcgtagctat cgcacccggc ggcaaaccgg taatggtata ggtgttatac
    13981 gctgtcggcg tttccaggtc tgcacgagaa agtttgccat tataacgctc tcccatcccg
    14041 taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt gataaatacc
    14101 gaggcaacct gatcgcgttc actggcaacg gcggtttctt tttcgataat tgatgccatc
    14161 gtcaccagct gatttttatc tttataaggc agaccgtccg cacgcccttc ccaggcgcta
    14221 tcgaccgctt tcaccatttt cttgtgcgct cgcttgagta acgcgacatc ggtggtattg
    14281 gcggtataca tccaggtgtc tggccagaac caaccttcaa tccactccgg gttttccagt
    14341 tcaagtgcct gcgctacggt ggcgtactta tcgtcactca gcgtgtgctt gatatacgga
    14401 gcctcacgca attgcttgag gtagtcgctc agacgcatcc cttctaccag tcgcagcggg
    14461 aactgtgctt ctttaccgct ttccagcaat ttcagcatct cgcgcacggt catctgcggt
    14521 gtaaagcggt aagtcccggc tttaaagtga gaaagatccg gttcgatacg cagcaaccat
    14581 tgaaaaaccc gtgggcgatt gatgatctta tcggcataaa gctgttcacc aagcgccaga
    14641 cgtccggtcc ctggcttcag ggtaaatatc gtctcttctt tgataagcaa tttgctgtcg
    14701 gcaagatggc gaaccttcca gacgcccaca ccagcggcga tacccagtac caccagcaat
    14761 aacaagataa tcaataacac ttttttcatg actaattcgg gcgctcacaa agtggggcta
    14821 aatattcata taacgttgct gacgaaaagg agacatcgcc acaggcacgt acgggcatca
    14881 ctggcattaa cgcattacaa ataaccatct catctgcctg caacgcctct tccagagagg
    14941 cttgcacttc gacaagctga taagaggatt gtgccagcaa acggatacag aattgtcgca
    15001 taatgccgtt aacacctgcc tgatccagac gcggcgtata aactacattg cccttccgcc
    15061 agaacaaatt agccgcacag cattccgtaa cccacccttc gctgtcaagg accagcgcct
    15121 catcagcgtt tgtctgctca agatgagagc ggatcaatac ttgctcaagc cgattaagat
    15181 gtttaatacc tgcaagatga ggattgcgcc ccagccgcac cgggcttagc gccaacgtca
    15241 tcccttcgtt acgcaaacgg tcgtaatgtg caggataaac cgtaacggag agaatccgcg
    15301 ttgccggtcc actgttcaat gtgctgtacc ctcgcccgcc actaccgcga ctgatcacga
    15361 ctttcagcac accattttgc tgttctgctg ccagcgtttt catctcttgt tcaagctgag
    15421 gccagaagtc acagaaaatc atcaaccgct gacaagcatc ctgtagtcgc tggatatgcg
    15481 ccgataacaa actgacttta ccgtcgataa ctctggcggt ggtaaaacaa ccatcaccaa
    15541 actgcgttgc ccgatcgctt actgccagcg attcctgctt atgaccgtta attaagaaca
    15601 taccggctcc ttatcagaga attgttagtg tggcagcatg ttcactacgg aacaagtcgg
    15661 aataaaagct aagaaaaaag gcccgcaagc ggacctttta tatgggtggg aaatgacaac
    15721 ttagatcttt ttaaagatca aagaaccatt agtgccaccg aagccgaagg agttacacag
    15781 cgtgtattcc attccgctga cctgacgtgc ttcatgcggt acgaaatcca gatcgcaacc
    15841 ttcatccggg ttatccaggt tgatggttgg cggaacagcc tgatcgcgca gcgccaggat
    15901 ggagtagata gattctactg cacccgccgc acctaacagg tgaccggtca tagatttcgt
    15961 ggagcttacc aacacacggc ttgcagcttc accgaagatg gttttcaccg cctgcgcttc
    16021 agctttatcg ccagccggcg tagaagtacc gtgcgcgtta acgtagccaa tctgactcgc
    16081 ttcaatgcct gcatcacgca gagcatttgc catcgccaga gctgcgcctg cgccattttc
    16141 tggcggtgac gtcatatgat aagcatcgct gctcatacca aagccgacga gttcagcgta
    16201 aattttcgca ccgcgttttt tcgcgtgttc gtactcttca agtaccagca taccggcacc
    16261 atcgcccagt acgaagccat cacgctcttt atcccacggg cggctcgccg cttgcgggtt
    16321 atcattgcgg gtagataatg cacgtgccgc gccaaaacca ccaacaccca gcggcgtact
    16381 ggctttctct gcgccacctg caaccatcac gtcagcatcg ccatacgcga taatacgcgc
    16441 agcatggcca atgttgtgca cgccggaagt acaggcggtc gcgatagaga tgcttgggcc
    16501 acgcaggcca tacatgatag tcagatgacc tgccaccatg ttcacaatcg ttgacggaac
    16561 gaagaatggg ctgatcttac gtggaccacc gttcatcaga gatgtgtggt tttcttcgat
    16621 cagtccgagg ccgccaatcc cggagccaat tgcggcacca atgcgggttg cgttctcttc
    16681 cgttatatca aggccagaat cctgcatggc ctgaacgcca gcgacaattc catattgaat
    16741 gaaggcatcc atcttgcgct gttctttgcg cgagataatg tcctcacagt taaaatcctt
    16801 tactaagcca gcaaatttcg ttgcataggc gctagtatcg aaatggtcga ttaggctgat
    16861 gccactctga ccggcaagca gagctttcca ggtagactct acggtattgc cgacaggaga
    16921 caacatgccc agtccggtca caactacacg acgcttagac acgtttgtcc tccagggagg
    16981 gaaaaaatga ttctagtggg acaaaaagat aaaactcagg cggtcgaacg accgcctgga
    17041 gatgttcact tacgcctggt ggccgttgat gtaatcaatg gcagcctgaa cggtggtgat
    17101 tttctcagct tcttcgtccg gaatctcagt atcaaactct tcttccagag ccattaccag
    17161 ctcaacggtg tcaagagaat ccgcgcccag gtcttcaacg aaagaagcat tgttggtaac
    17221 ttcttcctgc ttaacgccca gctgttcgcc gataattttc ttaacgcgtt cttcgatagt
    17281 gctcatactc ttaaatttcc tatcaaaact cgctttcgcg atggttttcg tagtgtataa
    17341 aatgttgaaa aatttgcaac taaatcccgg caggtcttac cacgatttta cgttattttg
    17401 aggccttacc ccccaataac gcaaatcatt ttgcactaat tattactcat aaccacgcag
    17461 actgcgcgca acatgagcat tttgtgcaaa tcgcggtcag accatgtaca tcccgccgtt
    17521 cacatgcaaa gtttcacccg tgatgtaagc tgcttcgtcg gatgccagga atgcaaccgc
    17581 gttggcgatt tcctgtgcgc cgccgaggcg acccgcagga acctgcgcca ggatacccgc
    17641 acgctggtca tcgctcagcg cacgtgtcat gtccgtttca ataaagcccg gagcaacaac
    17701 gtttacagta ataccgcgtg acgcaacttc gcgcgccagt gatttactga agccgatcaa
    17761 gcccgctttc gccgcagcgt agttggcctg accgccattt cccatggtac caaccacaga
    17821 accgatagtg ataatacgac catgacgctt tttcatcata gcgcgcatta ccgcttttga
    17881 cagacggaaa acagatgaaa ggttggtttc gataatatcg ttccactctt catctttcat
    17941 tcgcattaac aggttatcac gagtgatacc ggcattattg accaggatat ccacttcacc
    18001 aaattctgcg cgaatttttt ccagaacaga ttcgatagat gccgggtcgg tcacattcaa
    18061 catcagacct ttgccgttgg cacctaaata atcgctgatc gcctgagcgc cattttcact
    18121 ggtcgcagtg ccaataactt tcgcgccacg ggctgcgagc gtttcagcaa ttgcgcggcc
    18181 aattccgcgg cttgcaccgg ttaccagtgc gatttttcct tcaaaattca tgattttcct
    18241 cttttaaagc tcaagcgccg ctgccatcgc tgaaggttcg ttcagcgccg aggcggtcag
    18301 ggtgtcgaca atgcgtttcg tcaggccagt aagcaccttg cccgggccga cttcatagag
    18361 atgttctacg ccttgcgctg ccatgtactc aacagacttc gtccactgaa ccgggttata
    18421 caactgacgt accagtgcgt cacggatggc atcaccattg gtttcgcatt tcacatcaac
    18481 gttattcaca acagaaactg ttggtgcgtt aaaggtgatt ttcgctaatt ctactgccag
    18541 tttgtcggct gctggtttca tcagcgcaca gtgagacggt acgctcactg gtaacggcag
    18601 agcacgtttt gcgcccgcag ctttacaggc agcgccagca cgctcaaccg cttctttatg
    18661 accggcaata accacctgtc ccggagagtt aaagtttacc ggagaaacga cctgacctgc
    18721 tgcagcttct tcacacgctt tcgcaataga cgcatcatcc agaccgatga ttgccgccat
    18781 agcgcccgta ccttccggta cggcttcttg catgagcttg ccgcgcatct caaccagacg
    18841 caccgcatca gcgaaatcaa tcacaccagc gcaaaccagc gcggaatatt cccccaggct
    18901 gtgaccggcc atcattgccg gtgctttacc gccctgctgc tgccatacgc ggtacagcgc
    18961 aacagaggca gtcaacagcg ctggctgggt ttgccaggtt ttattcagtt cttcagctgg
    19021 cccctgctgg gtcagcgccc acaggtcgta gcccagcgcc gcagaagctt cagcaaacgt
    19081 ttcttcgaca attggatagc tcgccgccat atcagccagc attccaacgg tttgagaacc
    19141 ctgtccaggg aacacaaatg caaattgcgt catgttttaa tccttatcct agaaacgaac
    19201 cagcgcggag ccccaggtga atccaccgcc aaaggcttca agcagaacca actgccccgg
    19261 cttaatgcgc ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg ccgcagaggt
    19321 attaccgtgg cgatccagcg tcaccacgac attgtccata gacataccga gttttttcgc
    19381 cgttgcactg ataatacgca ggttagcctg atgcggaacc agccagtcca gttgagaacg
    19441 gtcaagatta tttgccgcca gcgtctcatc aacgatgtgc gccagttccg ttaccgcaac
    19501 cttgaagact tcgttgcccg ccatcgtcag atgaattgaa ttctctggat tcacacggtc
    19561 agcattaggc agcgtcagca actcaccata gctaccgtcg gcatgcagat gggtggagat
    19621 gattcccggc tcttcagagg cagccagcac cgcagcgccc gcgccatcgc caaaaataat
    19681 aatagtccca cgatcggttg gatcgcaggt gcgcgccagt acatcggaac cgacgaccag
    19741 agcatacttc accgccccag atttcacgta ttgatcggct acgcttaatg cataggtgaa
    19801 acctgcgcag gctgctgcaa cgtcaaatgc cgggcaacct ttaatgccca gcatgctttg
    19861 aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca caacgatcag
    19921 gccaatctgg tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg cttcaaagcc
    19981 catggttgaa acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac cggtacgagt
    20041 gacaatccac tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg tccgcacttg
    20101 ttcgggcaga tagctgccag taccaataat cttcgtatac atgtacgctc agtcactttt
    20161 cggttatata ccgtcacttg caaactgcga gttcgctggc agcgtcctgc taccgcagag
    20221 ttccgctttt gccaccgtcc agcagctcaa aaccagctgg gtatacagat tccaggcgag
    20281 cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca atcgcgaccg
    20341 caaaagctcg ctgattggct gcaccatgac tttttatcac cgtgccgcgc aatcctaaca
    20401 gacaggcgcc gttatactgg tcggggttga ggtgactgaa tcgcctcgtc aggctctttt
    20461 gtagccaacg ctttaataac agtagccacc acgaccgttt tttcccttca ccctgagatt
    20521 tcagcagaga aaggaacatc ctgacaacac cttccatcgt ctttaatgtg acatttcctg
    20581 taaagccgtc acaaaccagc acatctgtct tgccagttaa caactcattg gcttcaagat
    20641 agccgatata attgatagaa gggattgttt taagcaccgc tgaggcatcc cgaatactgt
    20701 cgagaccctt tacttcttct tcaccaatat tgagcaacgc cacgcgagga ttgggaattt
    20761 ccaccacctc ttcagccaga actgagccca taatggcaaa ttgcaccaac attgtactgt
    20821 cacaatcgac gttggcccct aagtcaagga ccaccgtttt gcccttttgc tgatgtggta
    20881 ataccgtcac cagcgccgga cgctcaatcc cctccagggg cttgagtaat aattttgcca
    20941 gccccatcag tgccccggta ttaccggcac tgacacaggc ttgcgctcga ccttctttca
    21001 ccagctccag ggccacgcgc attgaactcc cacgactggc gcggatagct tgcgaaggcc
    21061 gggcatcact ggcgataact gactgcgcag gaataatctg cagacgcgaa cgttgttcaa
    21121 agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa agaagagtga
    21181 gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa gggccaaaat
    21241 cccctcccat gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg ccgcccggtt
    21301 tggtctttcc ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc agacgcgtga
    21361 ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt ggtgacgcag
    21421 gtgtttttca ccagaagttt tgtctacaga caggctggtg actgcggtca gcgcgtcatg
    21481 ggaacgacgc atgccacgtt tggaacgggt tggtttattc tgttgtacgg ccatggacct
    21541 tactcctcaa ttacggttta tccccgatcc aacggggagc accaattact tacgctttaa
    21601 gctggctaat acggcaaatg ggtttggctt ttgcacttct tcaggcagtt caccaaagac
    21661 catgtccgct tcggacactt cacagtgttc agaatcatgc accggaacta ccggcaaggc
    21721 gaggatgatt tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt cgttaacctc
    21781 aatcggttca tacgcttccg gcagtgcttc agcctgttcg tctgaacgca caggactaaa
    21841 acaatacgtt gtgtagacct gatgagtaaa cggcttcccg caacgctgac actcgagcgt
    21901 taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga tagcgaacga
    21961 catggagcat tccacatcac tgtccacact gactacggat tcggcgacgc gctcaacctg
    22021 atcaggggta tagataccct ggtaatcaag gcgtttttga gccgtacgaa ccggatcgag
    22081 agtcaggggt aattttacct tttgcatagg gcgcgcatat taactttgta acgtcataga
    22141 gtcaaagaaa aaggcagcct gtggttgcct tttgccaata attcgcacac attgcgggtt
    22201 actgctttat tttcgttcag tgctgataac gaacatcaac ctgcaaaacg aagcgcatag
    22261 tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa aaaatatgcc taaacttatt
    22321 ttagcctcca cctcgccatg gcgtcgcgct cttctggaaa aactgcaaat ctcttttgaa
    22381 tgtgcagcac cagaggtcga cgaaacccca cgcagcgacg aatcaccgcg acagttggtg
    22441 cttcgactgg cacaagaaaa agcgcaatct ctggcgtcac gttatccgga tcatttaatt
    22501 attggttcag accaggtatg tgttcttgat ggcgaaatca ccggtaaacc gttaacggaa
    22561 gaaaatgccc gtctgcaatt acgcaaagcc agcggcaata tcgtcacctt ctatactgga
    22621 ctggcgttgt ttaattcggc gaatgggcat ctacaaacag aagtggagcc ttttgacgtc
    22681 catttccgtc acctgagcga ggcggagatt gataattacg tgcgtaagga gcatcccctg
    22741 cactgcgcgg gtagctttaa gagtgaagga tttggcatta cgctgtttga gcgtttagag
    22801 ggacgtgacc ctaacacgct ggttggttta ccgctaatcg cgctgtgtca gatgttacgt
    22861 cgggaaggga aaaacccgct gatgggatag ttgtttgcgc tggatgcggt tacgtcgcat
    22921 ccggcaatcg gtgcaggcgg cctgatgcgg cgtaaacgcc ttatcaggcc aaccgcccat
    22981 tatcaggctt atatgcttgt attagcgcgc gttacgcagc ttttgcaaac aacgcttcaa
    23041 accttcatcc atcggcgctt cgatacgcat cacctcaccg gtccccggat gggtaaactt
    23101 caacgctgcg gcgtgcagga acagacgatt taatcccgtg cctgcttcag tgagctgtct
    23161 gtcaaattca cggtcaccgt agcgatcgtc aaaggcaatc ggatgacccg catactgtgt
    23221 atgcacacgg atctgatgag tacgtccggt taccgggcta caacgcacca gggtggcaaa
    23281 tgcatagcgt tcttccactt taaagcgtgt ttccgacggt ttgccttcct gactgacacg
    23341 cacgatacgt tcgccgcttt gcagaatatt tttcaataac ggcgcttgaa cgctcttcac
    23401 atgcgactgc cactgaccgc gcaccagcgc caggtaatct ttttgcattc ctttttcacg
    23461 taattgctca tgcagagaac gcaacgccga gcgttttttc gctatcagca acacacctga
    23521 agtgtcccgg tcgagacgat gaaccagttc aaggaaccgc gcttccgggc gcaacgcccg
    23581 caaaccttcg ataacgccga agcttaaacc actgccgcca tgtaccgccg taccggaagg
    23641 tttattcagc accaggatat gatcatcttc atataagatg acgtccgcca gcgccgccac
    23701 cttttgcaga tgtggcgaaa ccgcctcttc ttcccgctca gcaacgcgaa ccggtgggat
    23761 acgcacctca tcacccgctt cgagtttata ttcaggctta atacgttttt tgttcacccg
    23821 cacttcgcct ttacgcaaaa tacggtaaat catacttttt ggtacgcctt tcaattgggt
    23881 acgcaaaaag ttatcgatac gttgccccgc ttcgtcagcg gtgatagcaa caatttttac
    23941 ggatggagtc tctgttttca tggtgggcga ttctaattag ccaacaggat tcgcgccact
    24001 catttttcta tgcttatatt tagttttgca ccttattact tcactacgtg atcactttat
    24061 tgatggttat taaaccaatc accagcaaga agtgaaaaaa ctgtgagtaa gcgggtgata
    24121 aatggtaaaa gtcatcttgc tataacaagg cttgcagtgg aataatgagg ccgtttccgt
    24181 gtccatcctt gttaaaacaa gaaattttac ggaataaccc attttgcccg accgatcatc
    24241 cacgcagcaa tggcgtaaga cgtattgatc tttcaggcag ttagcgggct gcgggttgca
    24301 gtccttaccg gtagatggaa atatttctgg agagtaatac ccagtctgtt tctttgataa
    24361 ttgcgctgtt tttccgcatg aaaaacgggc aaccgacact ctgcgcctct ttgagctgac
    24421 gataaccgtg aggttggcga cgcgactaga cacgaggcca tcggttcaca cccggaaagg
    24481 cgttactttg cccgcagctt agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa
    24541 gaatgttaat caacgcaact cagcaggaag agttgcgcgt tgcccttgta gatgggcagc
    24601 gtctttatga cctggatatc gaaagtccag ggcacgagca gaaaaaggca aacatctaca
    24661 aaggtaaaat cacccgcatt gaaccgagtc tggaagctgc ttttgttgat tacggcgctg
    24721 aacgtcacgg tttcctccca ctaaaagaaa ttgcccgcga atatttccct gctaactaca
    24781 gtgctcatgg tcgtcccaac attaaagatg tgttgcgtga aggtcaggaa gtcattgttc
    24841 agatcgataa agaagagcgc ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg
    24901 cgggtagcta tctggttctg atgccaaata acccgcgcgc aggtggcatt tctcgccgta
    24961 tcgaaggcga tgaccgtacc gaattaaaag aagcactggc aagccttgaa ctgccggaag
    25021 gcatggggct tatcgtgcgc accgctggcg taggcaaatc tgctgaggcg ctgcaatggg
    25081 atttaagctt ccgcctgaaa cactgggaag ccatcaaaaa agccgctgaa agccgcccgg
    25141 ctccgttcct gattcatcag gagagcaacg taatcgttcg cgcattccgc gattacttac
    25201 gtcaggacat cggcgaaatc cttatcgata acccgaaagt gctcgaactg gcacgtcagc
    25261 atatcgctgc attaggtcgc ccggatttca gcagcaaaat caaactgtat accggcgaga
    25321 tcccgctgtt cagccactac cagattgagt cgcagatcga gtccgccttc cagcgtgaag
    25381 tccgtctgcc gtccggtggt tcgattgtta tcgacagcac cgaagcgtta acggccatcg
    25441 acatcaactc cgcacgcgcg acccgcggcg gcgatatcga agaaaccgcg tttaacacca
    25501 acctcgaagc tgccgatgag attgctcgtc agctgcgcct gcgtgacctc ggcggcctga
    25561 ttgttatcga cttcatcgac atgacgcctg tacgccacca gcgtgcggta gaaaaccgtc
    25621 tgcgtgaagc ggtgcgtcag gaccgtgcgc gtattcaaat cagccatatt tctcgctttg
    25681 gcctgctgga aatgtcccgc cagcgcctga gcccatcact gggtgaatcc agccatcacg
    25741 tctgcccgcg ctgctccggt accggtaccg tgcgtgacaa cgaatcgctg tcgctctcta
    25801 ttctgcgttt gatcgaagaa gaagcgctga aagagaacac ccaggaagtt cacgccattg
    25861 ttcctgtgcc aatcgcttct tatctgctga atgaaaaacg ttctgcggtg aatgcgattg
    25921 aaacgcgtca ggacggtgtt cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc
    25981 actaccacgt gttgcgcgtg cgcaaagggg aagaaacgcc gaccttaagc tacatgctgc
    26041 cgaagctgca tgaagaagcg atggcgctgc cgtctgaaga agagttcgct gaacgtaagc
    26101 gtcctgaaca acctgcactg gcaacctttg ccatgccgga tgtgccgcca gcgccaaccc
    26161 cagctgaacc tgccgcgact gtcgtagccc cagcaccgaa agctgcaacg gcaacaccag
    26221 cagctcctgc acaacctggg ctgttaagcc gcttcttcgg cgcactgaaa gcgctgttca
    26281 gcggtggtga agaagccaaa ccgaccgagc aaccaacacc gaaagcagaa gcgaaaccgg
    26341 aacgtcaaca ggatcgtcgc aaacctcgtc agagcaaccg ccgtgaccgt aatgagcgcc
    26401 gcgacacccg tagtgaacgt actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc
    26461 gtcgccaggc acagcagcag actgccgaga cgcgtgagag ccgtcagcag gctgaggtaa
    26521 cggaaaaagc acgtaccacc gacgagcagc aagcgccgcg tcgtgaacgt agccgccgcc
    26581 gtaacgatga taaacgtcag gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat
    26641 ctgttcagga aaccgaacag gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc
    26701 gtcagctcaa tcagaaagtg cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac
    26761 cggtggttga agaaactgcc gctgccgaac caattgttca ggaagctcca gctccacgca
    26821 cagaactggt gaaagtcccg ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag
    26881 agaacaatgc tgataaccgt gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc
    26941 gtcacctgcg cgtgagtggt cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc
    27001 agtcgccaat gccgttgacc gtagcgtgcg cgtctccgga actggcctct ggcaaagtct
    27061 ggatccgcta tccaattgta cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg
    27121 aagaagtaca agtgcagccg atggtgactg gggtccctgt cgccgccgct gtcgaacctg
    27181 ttgttagcgc accggttgtt gaagaagtgg ctgaagttgt agaagccccc gttcagattg
    27241 ccgaaccgca accggaagtg gttgaaacga cgcatcctga agtgattgcc gccgcagtaa
    27301 ctgaacagcc gcaggtgatt accgagtctg atgttgccgt agcccaggaa gttgcagaac
    27361 acgcagaacc ggtggttgaa ccgcaggaag agacggcaga tattgaagaa gttgccgaaa
    27421 ctgctgaggt tgtggttgct gaacctgaag ttgttgctca acctgccgcg ccagtcgtcg
    27481 ctgaagtcgc agcagaagtt gaaacggtag ctgcggttga acctgaggtc accgttgagc
    27541 ataaccacgc tacggtgccg atgacgcgcg ctccggcacc ggaatatgtt ccggatgcac
    27601 cgcgtcacag tgactggcag cgccctactt ttgccttcga aggtaaaggt gccgcaggtg
    27661 gtcatacggc aacacatcat gcctctgccg ctcctgcgcg tccgcaacct gttgagtaat
    27721 aattagctca agtaatcaag ccctggtaac tgccagggct tttttatttc atctttgaat
    27781 caacatatta aaattcatcg aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa
    27841 aaatgcccgc cccggcgatc ccagagcagg cagacaaaac atacccagtt tcatgatatg
    27901 ttttaaagcg aaattatttg ttgagctgga agagcgacaa tccctgcata tcggtaaatg
    27961 ctttatacga tgcctgcaat gccgtttgct gcatgatgta agatgaaata gttgcattcc
    28021 agtccacatc aaccagatcg ctcatctgct gcgtttgccc taaagcgcga tcgctaccta
    28081 atgaatccag cgactccagt tcgttcagct gcgtgcctaa ttccgcgcga acggtcagca
    28141 cattgttcag cgagtttttc agtccgcggt tggttttatc taacgccgca gcggcggttt
    28201 ctttatccgc ttcgctatcc gcgaccggcg ttttcagggc tgcgatggcg ctatccagca
    28261 tggcaaaaag attggtttca gaagcgctac cgtctggttc cgctaccgcg ttactggtaa
    28321 tactgtcgaa aattttgtcg cccgtatgtc ctatcaccat cgaacgcgaa gcatcgacct
    28381 gttgcttaat actttctgtt ccgccgacat aatcaccgtt agcctcacta aacggcgcag
    28441 tctctgtttt ataaccggca aaaatgtagc gcccattacc gtcagtggtg ttcgccagat
    28501 tcagcaactg gtcacgaagc ccctgaatat ccgtagccag cgaggcccgg tcatcgtcac
    28561 tcaaggtgcc attgctggcg tagacaattt tttcctgagc attctggata gcagtggtga
    28621 cctggctaag tacactctct tccagtgaca ctttttgagt ggcgaaagta cgcgccagcg
    28681 tgtactggct gttttgcgcc tgtgcctggg agagaacaac cgcttgtgat gcagcaatgg
    28741 gatcatctga agggttaacg actcgcttac ccgtcgacat ctgttcgccg tacttcatcc
    28801 attctgcctg agaattggtg ataccacgca tgttttgctg gtacatcatc tgggtactga
    28861 aacgcatttt atctcccctt agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt
    28921 ctgcagaacc tgcgcattcg ccaggtaata ctgctgaaaa cgttgcagat ttccgtactc
    28981 ctcatcgaga ttgacaccgg aaatcgactg ctgctgattg gaaagctgcg tcaccacatt
    29041 accttgcgtg gtgctactgg ttttcaacgt cgcggtttta ttaccgatat cactcactaa
    29101 cgaggcataa gcgtcgttaa aggatttcgc accgcccacc gttttactat tgctttgcag
    29161 atccagcagg gcctgaccgt tgcggttatc gctatcaccc gcatcttttt cgctcgccat
    29221 cgctattttc gcttcgtcgg tgattaatac atccatgttg acgatggcgt cacttactgg
    29281 tttcagcgtg aagctgtcgt taacggcagg cgttcctgta aacgtcaact ccagaccatc
    29341 aaatgccact ttaccgttgg catccggcgt caccgtaaaa gtggtattgc tggcaaggcg
    29401 ggtgacctgc cactgattat tatcgaacga gattttgtaa tctgtcgcca gtaccacgga
    29461 ggcatcagtt accgtggcac cgatcgcaac gtcacctttg tttttcgtgt tttgcagaac
    29521 cgcgggctta ccgatagtaa agaaatcttc accagcatcg ccgttagcat caaatccggc
    29581 tttgtgttgg gtgttgaaag cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg
    29641 cgtctggtcc agctcctgag aacggaatgt cagaatgccg cccagcgacc cggtattcag
    29701 taatttctcc gggatctcaa tattgcctgc cgtcccatca acataagcga cagtcgtacg
    29761 agaagggtca gcgctggaag gaactgccgc cagttgccgc gccgtacttc cctgaaccag
    29821 tgagtaacca ttggccaccg tgatgttata agtgccgcca tcctgaacgc tgacttctac
    29881 accaacaatc tggtttaatt cgctcaccag ttgatcgcgt tgatccagca gattgttagg
    29941 tgacgcccct gcccccacgc ctgtcaggcg cgagatttga tcgttcaggc tggcaatttg
    30001 tttagcgtaa ttgttgatct gatcaacgct ggcacctatc gcgatattga cctgtttgtc
    30061 ctggtcgcgc agatattgat cggtggtttt aaactgattc accaaccctt ctgatttccc
    30121 aatcagcgcc tggcgcgctg ccgggtcttc cgcgttactc accagcgttt gcaggctggt
    30181 gaagaaatcc tgcatctgtg ttgccagcga agaggtactg gtggagagca tattgtcgat
    30241 tttcgacatc tgctcatagc gggcggtcag gccgctactt tgcgtctgcg cagcacgtaa
    30301 ctggttggta ataaacgcat catactcacg ctgcacacca gaaacgtaga cgccattgcc
    30361 aacccagccg ccagcgccca acgtgctatt ggcctgcgcc ataatagtgg tttggcgggt
    30421 atatccggca acgttatagc tggagatatt attacttgcc gtatttaacg ccgcctgggc
    30481 cgcgttcagt ccgctcatgg cgttattgat caagctggac atggaggttc cttataagcc
    30541 ttcaattaca gagtattatc ggcagcgacc cgccggactt gagttattca gaacagatta
    30601 tcaatgttca tgctgtaggt tttgctcacc ttgtcgctta tcgatttcat ctgctggatc
    30661 atgttggtga gtttacgggc atagtgagga tcggtagcat accccgcgtc ctgtagggcc
    30721 tgcgccccct gctccgcact cgcggctgtc gtcacggcgg cgtagcgcgg gttacgtgtt
    30781 aacagcccaa cgtaatccga caatgcttcc agatacgagc tgtagacccg aaatttcgct
    30841 tttactttct tcgcttcgcc attttcatat tcagtcgtgg tgatttcagt aactggacct
    30901 ttccagttgc cagaggcttt gacgccaaac aggttatagc tcggctcgcc gttttcgcgg
    30961 cggatttgcc gttgccccca accagactcc agcgccgcct gagcgaggat caaatgatgt
    31021 ggcacaccgc tttgctggct tgccagttgg gcgggcagcg agagttgcgc gaggaatgct
    31081 ttactgtcac ccggcagcga atcatcgtag ttacgtggca cggccttttg caccagctgc
    31141 gaaagcgcct gattttgata acgcaccaca gtttcgagcg ggaatttcat cggtgctgct
    31201 ggcgtggact cctctggcaa tggttgttct ggcgtcatct gtttaaccat catctctgca
    31261 agccccagac ctttgcccgc cgtcatctgt tgggcaatct gctggtcata catactggta
    31321 tacaggcgag tgtgctcgct gctgaacagg ccatcttttg gtaaagcgtc gcgcatgctt
    31381 ttcaacatca tctgcacgaa catcccttcc acctgacggg ccaccggacg gatatttgcc
    31441 gccggatctt cgcccgcttt cgcctttagt tcgttgagtg attgcgcatc ccaggccgca
    31501 cttgccagta gtttgctgtc gctgatcatc agatgatttc cagttttgcc cgcagacatc
    31561 ccgcactttg cattgattgc agtatggaca tcagatccat cggcgtagcg cccagcgcat
    31621 tgagcgcgcg caccacgtta ttgaggctgg cgctggaacg tacgctttgc agcgaaccgc
    31681 cgctctggcg taaatcgatc tgcgtttgtg gagtaaccac agtctgtcca ccaccaaacg
    31741 gtgtatctgg ctggctgaca ttggcctgac gattgactgt tactgagaga tttccctgcg
    31801 ctaccgcgca gctgtcgagg gtcacttcgc gattcatcac caccgaaccg gtgcgcgagt
    31861 taatcactac tttagcgtcc tgcggggtga cattaacctg catattctgg atatctgcaa
    31921 ggaaacggac ctgggaactg ttgccacttg gtacgcgcac ctgaatagtc cgcgcatcta
    31981 acgcggtggc gctgccatat ccacgcacgc ggttgatggt gtcagcgatt tgctgcgcca
    32041 tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt cccgacgcca aactggctgg
    32101 gcaattcacg ttcaataacc gcaccattgg tgatccgtcc accgttcagt tggttcacct
    32161 gaacactgct accgccagcg gaggctcctg cgccgccaac cagaatattg ccctgcgcca
    32221 gcgcatacac ctgactgtca acgcccttaa gcggcgtcat caacaacgta cctccacgca
    32281 agcttttggc atttcccatg gaagaaacca ccacatcgat ggtttgcccc tgacgtccaa
    32341 acggaggaag tgactctgtc accattaccg cagcgacgtt ttttagctgc atattggtgc
    32401 ccgtcggaac ggtaattccc agctgtgaga gcatgttatt aagcgtttgt gtggtaaacg
    32461 gcgtctgggt tgtctggtca ccggtgccat ccagccccac caccagacca tagccaatca
    32521 gtgagttttg ccttaccccc tgaacactgg tgagatcgcg aatacgctca gcctgagccg
    32581 ccgtcgtaac cagtagaaga attaatgcag agagaaattt aatcaccaca gcctcactta
    32641 cattggcgac aggttaagga agaaacgctg caaccagccc atattttgcg cttcgttaat
    32701 gtagccattg cctacgtatt caatgcgcgc atccgccacc tgagtagacg gtacggtatt
    32761 gctgccgctg atagtgcgtg gattaaccac gccagagaag cgaataaatt cggtaccctg
    32821 attaatggca atctgttttt cacccaccac atgcaggttg ccgttgacca gtacctggtc
    32881 aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca ttggccccgc cctttccgtt
    32941 gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg ttaccaaaca gcccctgcaa
    33001 atagcgcggc acagtatcaa agccaaaatt agttttaccg tcacggctgg cattcgcaga
    33061 ggagcttttg ctggcgctga cgttctcctg caacacgatg gtcagcgtat cgccaatatt
    33121 gcgtggtcga cgatcttcaa acagcggttg atagccatag ttaatcggct gagcagactg
    33181 gaaaatagaa ccgttggcga cgggcgtcgg accgggaacc ggttgtgcac tggtcgcccc
    33241 ctgcaccagc ggcgtggagg gtatccaggc gcagccggtt agtgaaagca ccaacaagct
    33301 ggaaatggca taagtatgcg cagcgttttt ttgcatggct atcatcttca aaaatcagta
    33361 aaccggtgaa cctgccaccg gttaagcctt agagttgcgt cagtttttgc agcatctgat
    33421 cggtggtgga caccgcttta ctgttgattt cgtaagcgcg ttgcacctga atcatattga
    33481 ccagttcttc cgccacgttg acgttagacg tttcaacata cccttgatac agcagtcccg
    33541 cgccgttcag gcccggcgtg ctttcgttcg gtacaccaga ggattgcgtt tcggtgtaga
    33601 ggttttcgcc aatgctctcc agcccggtgt cattcataaa ggtggtgaga ttgagctgcc
    33661 caacctgaac cggagctgcc tggccttgtt gggttacgct gaccacgcca tcacgaccga
    33721 tggtgatact taacgcattc gccggaatgg tgatcgctgg ctgcacctga aaaccaccag
    33781 ctgcccgttc tgatccacct ggaaagagcc gtcacgggta taggctgatg aaccatctgg
    33841 caacatcacc tggaaaaagc cctgcccttt aatcgcgaca tctttgctgt tgttggtctg
    33901 cgacaggttt ccctggctgt gtaagcgttc agtggcgacc gggcgtaccc ccgtgccgat
    33961 ttgtaatccg gagggtaagg tggtttgttc ggaagactgt gcccccggct ggcgaatggt
    34021 ttgataaagc agatcttcaa acaccgcgcg ctgacgctta aaaccgttag tactgacgtt
    34081 tgccaggttg ttggcaatga cgtccatatt ggtttgctgg gcgtcaaggc ccgttttggc
    34141 gatccataat gaactgatca taaaatgtcc tgtagtggat tagtgacaaa gtgcatatgt
    34201 cttcttgccg gatgcggcgt aaacgcctta tccggcctac aagtgctcgc aaaatcgata
    34261 aattgcgatt gcccccatag acccgataaa cttgcgcatc gggcaactta tacttgtcat
    34321 gtatcctttc aattaactca tcgacagcag ttggttggca cggcctgcgt tatcatcgac
    34381 gctgctgatc accttcatct gcatttcaaa acgccgcgcg ctggcaatca tgtcgctcat
    34441 tgccgcaacg gcattgacgt tactgccttc cagaaccccc gacatcacac gcaaggttgg
    34501 atctgcctgc agtaccggcc ctcgcgtggc ctgggtttct gcgcttaaac gaaaaatgcc
    34561 gtcgtcaccg cgctgcactt cgctgcccgt ggctttcacc agtttcagac gccctactgg
    34621 cgcaactgta tttgccggat cgcccggatt gagcgccgag attgtgccat cggcagcaat
    34681 agtgatttcc gctccttccg gcacagcaat tggccccgcc tcgcctatca cctgatgccc
    34741 ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg ccattacgcg tatacccttc
    34801 gctgccgtca gcggtctgca cggccagcca gccatcctgc tgcaacgcga cgtccagcgg
    34861 gcgcgaggtg taatccattt tgccgggcgt catatctgcg cccggcgttg acgccgtgac
    34921 caacgtgcgc gtgggcagag aaagcccttc cactggcacc gcgcgtaaag cattcaactg
    34981 cgcgcgaaaa ccgggcgttg aggcattggc cagattactg gcggttaccg cctgttgatt
    35041 cagtgtctgg ctggctgctc ccatcgcggt ataaattgcg tgatccattg agctatcccg
    35101 tcagcgatta gcgtaagtta accagcgtgt tgaggatctg gtcctgagtt ttgatggtct
    35161 gggcgttaga ctgatagtta cgctgggcaa cgatcatatt gaccagttct ttactgagat
    35221 cgacattgga cgcttccaac gcaccgttgg tcagggtgcc aaagttgccc gtcccggctg
    35281 tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg ccttcggatg
    35341 ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt tgtgtttgtt
    35401 cgttggaata gttgccgaca accgtaccgt catcattgat ttgataactc accagatcgc
    35461 ccggtttgta gccattctgg gtggttgcca caatattgtt agcgccggta ttttgctgca
    35521 tggagttgag gaagctcagg ctaaacgtgg cgggatctgc gccgttaatt gcgccagtgg
    35581 taatattctc tgttggattt gaggtcagaa cgccattggc attaaacacc agcttcattg
    35641 caggctcggc tgtacctgtt ggatcactgc tatcctgggt gtagacctgc cagttattat
    35701 ccccggtctt cacaaagtag acgctcatgt catgagcatt accctgactg tcgaaaacag
    35761 tcaccgaacc ttttttgtta tagctatccg cattgctggc gctgaatggc gtaacagtag
    35821 gaagcggatc actggaattc aggttgatct gcatcgacgc cgtggtggta gttttcgctg
    35881 ccatcagggt attcgggatc gaaatattgg tcggattcgc cccttgctga atagtcggcg
    35941 gcgtaccggt tgccgggtaa cccgtcagct gtaaaccttg catattcacc aggttacggt
    36001 tttcatccag cttaaattgt ccgttacggc tgtagaacac cgaaccgttg ctgtctacca
    36061 gacggaaaaa accgttctgg ctgatagcaa cgtccagacc tcgcccggtg ttggtggtcg
    36121 tgccatcggt aaagtcctga gtgataccgg caacttttac ccccagtccc actttcgaac
    36181 cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa gccgtaggtg gcggagttgg
    36241 cgatattgtt gccaataaca tcgaggttgg tggcggcagc gtttaatccg ctaaccgctt
    36301 gagaaaaggc catgactgac tcctgaaatg tgaaggctta aattatctgc cgtacttcgt
    36361 cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt accgctgttg ccgcggagca
    36421 caccctgcac cagagcaaac tgcagcggct gggcaaccag ttgtgtacca ccgttactgg
    36481 cgctaatcgc tacattgtaa gaaccgttcg gcgcagtgct gccatcagtc aacgtaccgt
    36541 cccaggtgaa actgtggact ccggcggtca gttcaccaat atcaatggtg cgcacaaccg
    36601 cgccattttt atcggtgatg gtggccgtaa ctttgtctgc cgcctgttgc agctcaacac
    36661 caaacggcgt ggtcgtggtc acagcccctt cttcactgcc ggttcccgca agaacagtgg
    36721 tgccggggat catcacgccg tgaccgatca ggttactggc ctgtaacgac tggctgttat
    36781 caatctgtcc ggaaatagat ccgagcgtgg tattgagttt ttcaatcccg ctgaccgtgc
    36841 tgatttgtgc caattgcgac gtcagctcgt tgttttccat tggattggtc gggtcctggt
    36901 ttttcagctg cgccaccaac aaagtcagaa aactgctttg taagtctgcg gcgttgctgc
    36961 ccgtgagcga actactgctg gtggtactga cgccggtatt tgtcggatcg gtggtggtta
    37021 ccgcaatgga catagctttc tcctttattg accgagcgta agggttttca gcatcatgct
    37081 tttcaccgtg ttgagcactt caacattggc ctgatagctg cgtgacgccg acatggtgtt
    37141 aaccatctcc ccgacgacat caacgttcgg catttttacg tagccctttg catccgccag
    37201 cggattaccc ggttcataaa ccagtttgtc cggggcctga ctttctataa catcggcaac
    37261 ctttacgccg cctgtcgcag cacctggtgc agcgttaacc tggaatacca cctgttttgc
    37321 ccgatatggc tgtccatcgg gaccggtcac gctatcagca ttcgccagat tactggccgc
    37381 cacgttcaag cgctgggact gggcagttaa cgccgacccg gcgatatcaa aaatattcag
    37441 cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga tttgcccgct
    37501 caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg tgcgttcgcg
    37561 atccatatcg acggtattac cgtcaagcga aggctggtcc ggaatacggt attgcagttc
    37621 tgcggtagga ggcgtcagcg cctgcgccgg aatgtgttgc gttgaggtca tcgtcagtgc
    37681 aaccacactg gttgcatccc gtccacgttg catgactttt ttaagttcac tggcaaaatc
    37741 gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg ccagcacttc
    37801 ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg cgtcgagctt
    37861 atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat gcctttacgc
    37921 gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg atgaccattg
    37981 cggacaggta caattcacgt tgtagaaatg gctgggggcg aaaatgctgg caataaaacg
    38041 tagcgtggcg atcatctcga tactattcac tccgttaagt gcggcgagta atctcacatc
    38101 gcaattgcaa acctttttta gcgcccagct cgcgggggta agtgatgagg ttcgtgtttc
    38161 tattcgtaca gcgcccaatc tactaccgcc gtgcgagcag ccattgcttt cgatgagcaa
    38221 taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg acaaacgata
    38281 tttacaggtt aatgtacagg ccacaggaaa ttatgtggtt gccgcgatgc ccattgtgcg
    38341 gggaggaaag ctggaagctg gcaatgtcaa actaaaacgc ggacggcagg ataccctgcc
    38401 accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc gcgatctatc
    38461 acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa aagcgggaca
    38521 acgcgtcaac gtgatcgcca gcggtgacgg gtttagcgcc aacgcagaag gtcaggcgct
    38581 gaacaatgca gccgtcacac agaatgcgcg ggtgcgcatg gtatcgggac tggtggtcag
    38641 cggcgttgtt gatgcagatg ggaatattct tataaaccag taagctgtta aagattaccc
    38701 gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg aataaaccat
    38761 gagtattgat cgcacttcgc ctctgaagcc cgtaagcacc gttcaaccac gcgaaaccac
    38821 tgacgcgtcg gtaacgaaca cccgggcggc aaaaacaaca gcctccacca gcaccagtgt
    38881 gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata tcaatcttga
    38941 acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg acaccggcaa
    39001 aattgccgat gcgctgctca acgaagcgca gcaagacttg cagagtaact gatcgtatga
    39061 cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc aaaacggtaa
    39121 tggatcaaga acagcaacat ctctctatgg ggcagatcaa cggcagccag ttgcaatgga
    39181 ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag ttacgcagga
    39241 aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa attactgtga
    39301 aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa ggacagattg
    39361 agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg ctatatgggg
    39421 cgaacggtca gacctcaaca acccatcgcg gcggtaaaaa gatttcgatc tgaatattat
    39481 ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc aaattcttta
    39541 actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc cagcacgacc
    39601 gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg catgatatgt
    39661 aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg caacagaaac
    39721 gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca gtaaagcagc
    39781 gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccagcatg tttcaacgga
    39841 ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc aattttcact
    39901 ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca acactttcac tacaatcagg
    39961 ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc atcaaacgcg
    40021 gtaaatttac cgtactggaa cagcgaaacg gttaacggac ccgaaagaat gcccaacgca
    40081 accgcactcg gcagcgccaa caggaaacaa agacgcaacc cccagtccat caaacggttg
    40141 tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa aatggtgcca
    40201 agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta atacatccaa
    40261 gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa ggagatctgg
    40321 ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat tgctccggca
    40381 tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg cggtagctga
    40441 tacaccagct gcaggatgcc gccgaccgta acagcccacg ccagcgccag caccggtggg
    40501 ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag cagtgttggc
    40561 gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac cagcgacgcc
    40621 agggagatca gcaagatata gggaaaggta atctttagta gctggctggt cagggcaaat
    40681 ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg tgcggcgagc
    40741 atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac ataagagaca
    40801 aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat cggtacaaat
    40861 gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag tttaaaagcg
    40921 acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat tgcgtctcgt
    40981 gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag cgattttaat
    41041 aaattcatcg gtgttctaat ccagacccac gggcaaaacg cctgcaaggc aggcgctgaa
    41101 agaggcgaat agtctacctg gattatggtg aattgctacc gccagatgtt acagggttta
    41161 ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca ctgcctgttc
    41221 gccggcggtt tgcagaactg tctgattttg cacacattca atgaagtgcc gcgcacagcc
    41281 gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat gcactacgcc
    41341 ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg catcgtcagt
    41401 cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacagg tggtgatctg
    41461 caaaggatcg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt cgttagtcag
    41521 tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg tatccaccac
    41581 atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacac tattgctacg
    41641 atgtttatcc attcttagcg aagctgcggt agcgagttgc gtttttaact caccgtagag
    41701 tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg ccgccagttc
    41761 caccagccgt tcagcatcgc gcagattttc tgccagcggt ttatcgacac agacatgtac
    41821 acccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg aatgcacaaa
    41881 aaccgcatcg caactggcgg caaggctgga taacgaatcg gcataaggaa tgcgccagct
    41941 ttcacaaatt ggcagggctt tcgcgcgcgt aggcgaccag gctccttgta acatccagtc
    42001 agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta atcccacaac
    42061 gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa tcaagacgct
    42121 gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggcctgtaaa tcaccatcaa
    42181 ccgcgttcga catatccgtt accgccggct gatcctctac ctcaccactg aaaagatgca
    42241 tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa ggaccatctt
    42301 cgcgatttgc cagttgttcc agcgtcgact ccacttccgc catatcgctg aattcataca
    42361 ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc accacgcaat aacaacgtgg
    42421 tgattaacgc cacttccgct gcgctcagtt tcagatcgcc aaattctgaa ttacaaaaac
    42481 gttgttcata tttggtgacc cgattaccaa agccactcac cgtgcgcaga taatggcgtt
    42541 tgaccagatt atccagttgc tcctgcactt cagattcact cagattcatg accggttcac
    42601 ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcggg tattgctccg
    42661 gcgtggtcac ctgcttttcc agcaagcaac caatcacgcg agcttcaagg gcagtaagtt
    42721 gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt taatgccgtc
    42781 agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata gccttctttt
    42841 tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat ataattagcc
    42901 ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt cagggcttca
    42961 aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata gcaggcatga
    43021 aaagagccac gaacaacatt ggaaaaattg gcaacgccaa taatctcttt ttcgtccggg
    43081 tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat catccccagc
    43141 ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc ccagggcttg
    43201 aggaaatggc gattctctgc gtaataatcc gcaagacgcc aggcatcacg atcatgcacc
    43261 agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact gcgatagcca
    43321 aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta ttactatacc
    43381 tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt cacatatttg
    43441 ataaaaactc tctatcaaag cgacgttttg ataaaaaaat attgtccccg aagtgatggg
    43501 aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc gggaggggaa
    43561 atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat cgataatatg
    43621 ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt cgttgatcaa
    43681 atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt tattcagcaa
    43741 ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc gatgattgtt
    43801 accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca cgaaccgtgg
    43861 ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga tccgccgcgt
    43921 tcggcgctgg tggtgaaact aatccgtcca caacaacgtt gtcgtttttt ctcgctgttg
    43981 atgatgcagg acagtgccgg tgcggtcatt ggcgcattgt tggggagctg gctgttgcaa
    44041 tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctgtg tgcggcgttc
    44101 aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt tcgcgaaggc
    44161 atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct ggcgggttac
    44221 tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga cgtggctggc
    44281 gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt aacgttgctc
    44341 taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt gatggctggg
    44401 ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggtct gcaacaactt
    44461 ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc gcgtgaaacc
    44521 ttaagtgctt tgctggcgga cgcaagagct cgcggcagct atatggggtt tagccgtctg
    44581 ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt tgacctgggc
    44641 aaatcggcgc accagccaga gcttccgtgg atgatgctgg gaattattgg catcttcact
    44701 ttccttgcgc tgggttggca gtttagtcag aaacgcgcaa cgcgtcgttt gctggaacgc
    44761 gacgcctgat ttgatactcc cccctgactc ctccatactg aaagtgaact gacagccaaa
    44821 tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct caaagcccgc
    44881 atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa cgacgacgca
    44941 gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa agatgacagc
    45001 cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga cggcaaaccg
    45061 ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt caatggctac
    45121 gccaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt ttctactccc
    45181 gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt tgccgacctg
    45241 ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc gctggacaaa
    45301 ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat tcagctattt
    45361 ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg cgttgctgat
    45421 taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat ggcgatttaa
    45481 tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt tttttctgct
    45541 ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg cagatttcat
    45601 gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca gtggcctgct
    45661 cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta accagtcgct
    45721 tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg acgcccatat
    45781 tgttctgaca aacttgatca gccaaattgg tcgcgaagag ccgaataagg ttaccctaac
    45841 cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga ccatgaaact
    45901 gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc tgaaagagat
    45961 ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc aaacgttgct
    46021 tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt acgtcctgcg
    46081 cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca ttgaagtgaa
    46141 gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc gcgcccttta
    46201 tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg tccggcaaaa acattccttc
    46261 agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc gccgcccaga
    46321 cgactggcac cttcacctcc gcgatggcga catgttaaaa actgtcgtgc cgtataccag
    46381 cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga ccacagttga
    46441 ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc acgatttcac
    46501 cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg agcgcggatt
    46561 taatgaaggc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca ctaactccag
    46621 ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg aaaaaatcgg
    46681 tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt ttgatcgtga
    46741 agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg cgctgaaagt
    46801 cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg gaaatgaacg
    46861 gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata tgctggttgg
    46921 tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc accaacaggc
    46981 attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg attctgcgcc
    47041 acatgcacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca acgccccaac
    47101 cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc actttgaagc
    47161 attctgttct gtaaacggcc cgcagttcta tgggttgccg gtcaacgaca cattcatcga
    47221 actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg acaccctggt
    47281 gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat cgtagcttcc
    47341 tgttgtcatt aggttattta acctgtataa ataaccagta tattcaacag ggggctatta
    47401 tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct attgacgccc
    47461 tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa ggccacgtat
    47521 cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa gaagataaac
    47581 agcgcattag cgaaattctc caggaaacgt gggaaagcgc cgatgactgg tttgtcagcg
    47641 aataatatgc agtgattttt gtttttgccg ggtcgccccg gcttttttat tttacttacg
    47701 aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg ctaaaaaaat
    47761 atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt tatctgttcc
    47821 cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg cttcagataa
    47881 aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc catgatggaa
    47941 aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag caccgttgat
    48001 agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc cgagcaggaa
    48061 gggactgaag ttggtcaaac gctctggtta accactgatg ttgccagaca atttatttcg
    48121 atattagaag caggaatcgc caaaattgaa tccggtgatt tccaggtaaa cgagtatcgg
    48181 cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc tttaccattt
    48241 ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag cagagagaaa
    48301 aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc cgccgggtat
    48361 tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc accgcatcaa
    48421 cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga aggcgaaaag
    48481 tatgtcccgc tggtcctccg cgcgcaaacg ctgtgggatg aactttcccg ccacaacgaa
    48541 gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga ctccgcattt
    48601 ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa gctcgatgcg
    48661 caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat cggcttattt
    48721 gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat ccaactggcg
    48781 aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg tcatgacgat
    48841 gatggcgtaa ctattgaaac ggttgacggt gagtatcagg cgaaaaaagc gattgtctgc
    48901 gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt acgtaaagta
    48961 tttgcctggt atcaggccga tggccgctat agcgtgaaga ataaattccc ggcgtttacc
    49021 ggtgaactgc ccaatggcga tcaatattat ggttttccgg cagaaaacga cgcgttgaag
    49081 attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt tccgtttgcg
    49141 gaagtggtca gcgatggttc ggaagccttc ccgttcttgc gcaatgtatt gccgggtatc
    49201 ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga agattttatt
    49261 atcgataccc tacccggcca cgataataca ctgctcatta ccggcctgag tgggcacggt
    49321 tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga caaaaaaagc
    49381 gattttgatt tgacgccatt caggctttcc cgcttccaat aatcatcatt acggcctcca
    49441 gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat ctca
//
LOCUS       DAZPJR010000008        48840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPJR010000008 DAZPJR010000000
VERSION     DAZPJR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.009"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            34..663
                     /locus_tag="ACHVB7_000484"
     CDS             34..663
                     /locus_tag="ACHVB7_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR1912580.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHVB7_000485"
     CDS             complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHVB7_000485"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR1912581.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHVB7_000486"
     CDS             1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHVB7_000486"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR1912582.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3031..3228)
                     /locus_tag="ACHVB7_000487"
     CDS             complement(3031..3228)
                     /locus_tag="ACHVB7_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1912583.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHVB7_000488"
     CDS             complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHVB7_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR1912584.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHVB7_000489"
     CDS             complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHVB7_000489"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR1912585.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHVB7_000490"
     CDS             complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHVB7_000490"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR1912586.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHVB7_000491"
     CDS             complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHVB7_000491"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR1912587.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHVB7_000492"
     CDS             complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHVB7_000492"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR1912588.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHVB7_000493"
     CDS             complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHVB7_000493"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR1912589.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            10899..11864
                     /gene="fepB"
                     /locus_tag="ACHVB7_000494"
     CDS             10899..11864
                     /gene="fepB"
                     /locus_tag="ACHVB7_000494"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1912590.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(11945..13195)
                     /gene="entS"
                     /locus_tag="ACHVB7_000495"
     CDS             complement(11945..13195)
                     /gene="entS"
                     /locus_tag="ACHVB7_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR1912591.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13306..14310
                     /gene="fepD"
                     /locus_tag="ACHVB7_000496"
     CDS             13306..14310
                     /gene="fepD"
                     /locus_tag="ACHVB7_000496"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR1912592.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14307..15299
                     /gene="fepG"
                     /locus_tag="ACHVB7_000497"
     CDS             14307..15299
                     /gene="fepG"
                     /locus_tag="ACHVB7_000497"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR1912593.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15296..16111
                     /gene="fepC"
                     /locus_tag="ACHVB7_000498"
     CDS             15296..16111
                     /gene="fepC"
                     /locus_tag="ACHVB7_000498"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1912594.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16108..17241)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHVB7_000499"
     CDS             complement(16108..17241)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHVB7_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR1912595.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(17457..21338)
                     /gene="entF"
                     /locus_tag="ACHVB7_000500"
     CDS             complement(17457..21338)
                     /gene="entF"
                     /locus_tag="ACHVB7_000500"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR1912596.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="ACHVB7_000501"
     CDS             complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="ACHVB7_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR1912597.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(21556..22758)
                     /gene="fes"
                     /locus_tag="ACHVB7_000502"
     CDS             complement(21556..22758)
                     /gene="fes"
                     /locus_tag="ACHVB7_000502"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR1912598.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23001..25241
                     /gene="fepA"
                     /locus_tag="ACHVB7_000503"
     CDS             23001..25241
                     /gene="fepA"
                     /locus_tag="ACHVB7_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR1912599.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            25416..26036
                     /gene="entD"
                     /locus_tag="ACHVB7_000504"
     CDS             25416..26036
                     /gene="entD"
                     /locus_tag="ACHVB7_000504"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR1912600.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="ACHVB7_000505"
     CDS             complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="ACHVB7_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1912601.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="ACHVB7_000506"
     CDS             complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="ACHVB7_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1912602.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            26782..27050
                     /locus_tag="ACHVB7_000507"
                     /pseudo
     CDS             26782..27050
                     /locus_tag="ACHVB7_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27253..28371
                     /locus_tag="ACHVB7_000508"
     CDS             27253..28371
                     /locus_tag="ACHVB7_000508"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR1912603.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            28437..28685
                     /locus_tag="ACHVB7_000509"
                     /pseudo
     CDS             28437..28685
                     /locus_tag="ACHVB7_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            28750..29118
                     /locus_tag="ACHVB7_000510"
     CDS             28750..29118
                     /locus_tag="ACHVB7_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1912604.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            29212..29865
                     /gene="nfsB"
                     /locus_tag="ACHVB7_000511"
     CDS             29212..29865
                     /gene="nfsB"
                     /locus_tag="ACHVB7_000511"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR1912605.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            29973..31220
                     /gene="ybdG"
                     /locus_tag="ACHVB7_000512"
     CDS             29973..31220
                     /gene="ybdG"
                     /locus_tag="ACHVB7_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR1912606.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(31288..32664)
                     /gene="pheP"
                     /locus_tag="ACHVB7_000513"
     CDS             complement(31288..32664)
                     /gene="pheP"
                     /locus_tag="ACHVB7_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR1912607.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(32766..35909)
                     /gene="cusA"
                     /locus_tag="ACHVB7_000514"
     CDS             complement(32766..35909)
                     /gene="cusA"
                     /locus_tag="ACHVB7_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR1912608.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(35921..37144)
                     /gene="cusB"
                     /locus_tag="ACHVB7_000515"
     CDS             complement(35921..37144)
                     /gene="cusB"
                     /locus_tag="ACHVB7_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR1912609.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(37160..37492)
                     /gene="cusF"
                     /locus_tag="ACHVB7_000516"
     CDS             complement(37160..37492)
                     /gene="cusF"
                     /locus_tag="ACHVB7_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR1912610.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(37516..38898)
                     /gene="cusC"
                     /locus_tag="ACHVB7_000517"
     CDS             complement(37516..38898)
                     /gene="cusC"
                     /locus_tag="ACHVB7_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR1912611.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            39055..39738
                     /gene="cusR"
                     /locus_tag="ACHVB7_000518"
     CDS             39055..39738
                     /gene="cusR"
                     /locus_tag="ACHVB7_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR1912612.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            39728..41176
                     /gene="cusS"
                     /locus_tag="ACHVB7_000519"
     CDS             39728..41176
                     /gene="cusS"
                     /locus_tag="ACHVB7_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR1912613.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVSKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            41913..43814
                     /locus_tag="ACHVB7_000520"
     CDS             41913..43814
                     /locus_tag="ACHVB7_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFR1912614.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            43842..44303
                     /locus_tag="ACHVB7_000521"
     CDS             43842..44303
                     /locus_tag="ACHVB7_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR1912615.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            44323..48840
                     /locus_tag="ACHVB7_000522"
                     /pseudo
     CDS             44323..48840
                     /locus_tag="ACHVB7_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT        11693 a        13454 c        12815 g        10878 t
ORIGIN      
        1 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
       61 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
      121 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
      181 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
      241 ccacccgaaa aaaaactgct gcaaaaatcc attgaaatag atggttttac ccaacccatt
      301 gtggtcacac atacggataa aaatgccatg gaaatcgtgg atggttttca ccgccatgaa
      361 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
      421 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
      481 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
      541 gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa
      601 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
      661 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
      721 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
      781 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
      841 cgcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
      901 ttcatttaag gcatttacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
      961 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     1021 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     1081 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     1141 tgcccgctca cgcagctgcg aatgcgccag cacactggca tggccctgtt gtgaaaagtt
     1201 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     1261 caaagcggcg aaatcagcct gctgccagac agttgcactg gagttatgcg gagtgttgag
     1321 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     1381 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcgggggc
     1441 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     1501 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     1561 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     1621 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     1681 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     1741 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     1801 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     1861 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     1921 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     1981 tcacatccag gaagtttcaa tcacctgaac gattttttca ctgatgaaca actttctcgc
     2041 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     2101 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     2161 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     2221 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgtt
     2281 ccgacgatcg ccgctacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     2341 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaacca
     2401 gagattatcc tcaacgcgcc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     2461 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     2521 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     2581 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     2641 attgctggtg gtgggatggt cggtgatctg ggcgatcgtt ttacgcgtgt ggcggcggct
     2701 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     2761 aaagtcgctt atggcattct ggtacaaagc gccttgctag gtcaggatga tgtgctggcg
     2821 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaaatg
     2881 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     2941 tacattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     3001 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     3061 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     3121 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     3181 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     3241 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     3301 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     3361 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     3421 cttttgcctg cgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     3481 tcgccgttgg cttcggatct ttcaatgccg ccagtgccga cttaatcgag aacagcgcca
     3541 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     3601 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tctatcattg
     3661 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     3721 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     3781 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     3841 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     3901 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     3961 ggttagcagg cagtgaatcg gtccgcttca ggccaggaga caccacgccc agcagatcct
     4021 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     4081 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     4141 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     4201 actgttcacc cacttcgctg gcaatctggt tgagcgtatc cggagtaatc gcaaagcccc
     4261 agctactcac cacctgcgcg gcagaagcga ccacatccgc cgtccctgcc ggagccagca
     4321 ccgccatcgg gctgttcatg gcgaagtaca cgcccggatc gatgatacag gcggaaacca
     4381 gtgccataat cgccacgaag gattccatta acattccacc gtagccgata aagcacgcct
     4441 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagcctg
     4501 acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa cagattaccg gtccataccg
     4561 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcgga cgcataatca
     4621 aaatgcctac cgccagacca acgatagtcc cgattttcag gaaggtagag aggtagtcac
     4681 gcggggccag cagtaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     4741 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcggg ctttctgcca
     4801 cccagccgcc agagataatg gcaaaaataa ggaacaccag gccaatcact gacacttcgc
     4861 caatacgccc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     4921 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     4981 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     5041 cagttggccc catctcttct ttgaccagct cacccagcga gcgaccgtca cggcgcgtag
     5101 aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc
     5161 agatcatccc cggcaggtag cccatttgcg ctgccagcac cggccccacc agcggccctg
     5221 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     5281 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     5341 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     5401 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     5461 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     5521 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacgat
     5581 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     5641 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     5701 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     5761 tgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     5821 tgccttcaga catcgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     5881 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     5941 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     6001 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     6061 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     6121 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     6181 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     6241 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     6301 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     6361 gccgctaacc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     6421 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     6481 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctgaaaca ggttaaacgc
     6541 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     6601 gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa
     6661 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     6721 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcttc
     6781 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     6841 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     6901 gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc
     6961 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     7021 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttcctggcg
     7081 gggacaggtg ctggcagtaa tccttcagtc atcaccaccc ggccagaccg cccggccaca
     7141 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     7201 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     7261 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     7321 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     7381 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     7441 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg
     7501 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     7561 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     7621 gcggcacgtt gcggttcaaa ggcccagtca actttattct gcggaatatc gtgagactcc
     7681 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     7741 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     7801 aagtgaatcc acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     7861 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct
     7921 tttttcgccc atcagctcat cttccatgct caccagtgcg gcatagatca ccgccggatg
     7981 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg
     8041 atctttctcg cgcccctgca cggtgatgta accctctgga tcaatagaga tcagatcgcc
     8101 ggaacagtaa aaaccgttgg catcaaaggc actggcattg tgctgcggac ttttgtaata
     8161 accgcggaag gtgtacggcc cgcgcgtcat caggcgtcca acttcccctt gcggcagtgg
     8221 atttccttca gcatcagcaa cccatacttc atcgtccgga cacattgggt aaccctgggt
     8281 atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcaccagcc cttccgccat
     8341 gccaaacacc tgctgcaact ggcagccaat ctcagcggaa atacgcgccg caagggtggc
     8401 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     8461 gccttcagcc agcgcctgca accacaggct aactgccggc ggcaccagcg cggtaacgtt
     8521 aacctgatgt tttgcaatca acgggaagca aagcgtggcg ctgggatcgg ccgccagaac
     8581 aaccgttcct ccggcaagaa agacgcccag cgatcctggc gaactcatag cgtagttatg
     8641 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
     8701 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagcttcg gtgtaccggt
     8761 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tagcagtaaa
     8821 accgtctgcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag
     8881 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
     8941 aaacagcgca tgttggcgat cggcaatcag taacgccggt tcaatctggc tggcataggc
     9001 gttcagttca ctacgctgat ggctgaacaa cgccagcacc ggcgcaacgc ccagtttcag
     9061 cagtgcgaaa aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc
     9121 accgggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
     9181 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
     9241 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
     9301 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
     9361 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
     9421 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
     9481 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
     9541 gccaaacagt tcgcggtcaa acggctccag ttcagcaata acctgggtcg cggcctgatg
     9601 cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc
     9661 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
     9721 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
     9781 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
     9841 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
     9901 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
     9961 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    10021 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    10081 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    10141 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    10201 acagtgggct gaggtctgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata
    10261 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    10321 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    10381 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    10441 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    10501 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    10561 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    10621 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    10681 cattattgat gaattcgcat aagcgcaatg tgatgtcctg cgccgttttg ccccctctcc
    10741 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    10801 accctaaccc tctccccaaa ggggcgaggg gacggattgt gcgctttgtc gaatttgtca
    10861 ttacgccctt aaccttatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    10921 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    10981 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    11041 gtattgtttc caccagcgtc accctgaccg gatcactgct ggcgattgat gctccggtga
    11101 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcaga tgaccagggc tttttacgcc
    11161 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg
    11221 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    11281 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    11341 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    11401 aagcggcaga gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca
    11461 aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc
    11521 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    11581 agttgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    11641 ttggtgggga aaatctggct gctgggttaa acggtgaatc gctattcctg ttcgccggtg
    11701 atcagaaaga cgccgatgcg atttatgcca atccactgct cgcgcacctg cctgcagtac
    11761 aaaacaagca ggtttatacg ctgggaaccg agacgttccg cctggattac tacagcgcca
    11821 tgcaagtgct ggacagactt aattcactgt taaaaaaaga ttgataaaac atacaattag
    11881 agcactaata atcccctcgc ccctttgggg agagggttag ggtgagggga aaatgcacct
    11941 ttagctaact gtcagacgct gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca
    12001 ccagcaccag caataacaac acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg
    12061 aagcaaccgg tgtcatcatc gcgcccaaac cacccaacag cgccgcgcct atggcatcgc
    12121 ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc taacatcgct tccggggttt
    12181 gcgtttgcag cattgtatat tgcagcaacg aactaaccgc gctcagccag ccgaacagcg
    12241 ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca atggcgagga
    12301 acgatcccag cgtggagagc agcatcaata accctggtcg cgcactatgt gccagcttcc
    12361 cgctggttaa cgcaccaata gccgcaccga gcgggatcgc cgcatagaga aaaccaatct
    12421 gtgccgccga catctgccag ttgtcagcca gcgccggata cagtacccgc accgcgctcg
    12481 ccatcgtcaa taaaccaccc agcaacgcaa tccctcctac cagcgggctg gcgagcagaa
    12541 aacgaaatcc tgccagtaat gatttcaacg gatgctcgcg cggctgcggt ggcggtggca
    12601 acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc gccagcccgt
    12661 agttccaggc tacgccgccg gtcgccagca ataaaccgcc aatcatgggc gaaatcaccg
    12721 accccagacg cacggtcaac atggtgatcg ccccggcctg cattaagttt tcacgcccta
    12781 ccagtgcagg tgttgccgcc agtagcgccg taacaccaag tgatgcgaaa aaaccatccc
    12841 ataaaccaag taaatagatt gccagcaatg acggctccgg cagcagcgcg ttcaggcaca
    12901 gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt ttgcgctcat
    12961 agcgatccgc cagcacaccg ccaaccatca ggccaacaaa catcgcgccg ccggtcagcg
    13021 tcaccgaaag ccccacctgc caggtagaat gcgtcatcat ctggatctgc accggcaccg
    13081 cgacgccgag caaacccaga gacacaattg agataaaacg agcgaggaat actgcgcgaa
    13141 acgccgggtg cgttttcaac aggctgaggt taagcagcca ggattgttta ttcattacaa
    13201 tgccttgcca tcaaatattt taaataccta ttcttaggct gcacatgcta acatatccaa
    13261 ataagatcga taacgataat taatttcatt atcatggaag ttcgtatgtc tggttctgtt
    13321 gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat cgcgacggca
    13381 ttaagcctgc tcattggggc aaaatcactc cccgcgtcag tagtgctgga ggccttctca
    13441 ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg cgcgactgcc gcgcaccctt
    13501 gccggtttac ttgcaggcgg tgcgcttggg cttgctgggg cgttaatgca aaccctcacc
    13561 cgaaatccac ttgccgaccc cggcttgctt ggcgtgaacg ccggagccag ctttgccatt
    13621 gtgctgggtg cggcgctgtt tggttactca tccgcgcagg aacaactggc gatggccttc
    13681 gccggggcgc tggtggcctc attgattgtt gcctttatcg gcagtcaggg cggcgggcag
    13741 ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct ggaagggctg
    13801 accagcggca tcgccctgct taatcctgac gtctacgatc aactgcgttt ctggcaagcc
    13861 ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc ggttctgatc
    13921 gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca gtttgagcct cggcagtgac
    13981 accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt tgattggtct gctggcgatt
    14041 accgtgcttt gtggtagtgc gacggcggta gttggcccga ttgcctttat cggcctgatg
    14101 atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct gcccgtcacg
    14161 ctacttgcta cccctgccct gctgctgttt gccgatatca tcgggcgggt gattgtgccc
    14221 ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg caccggtgct gatcttcctc
    14281 gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc tcgccgatta ctcatcacct
    14341 gtttgctgct gatttccgcc tgtgtggttg caggtatctg gggattacgc agcggtgccg
    14401 tcacgctgga aatctcgcag gtattcgccg cgttgatggg cgatgcgccg cgcagtatga
    14461 cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg
    14521 cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc ggcagccctg
    14581 acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg
    14641 gtcaggacct gacggctatc gcgctggcag caatggtggg cggcattgtc acttctctgt
    14701 tggtctggct gctcgcctgg cgtaacggta tcgacacctt tcggttgatt attatcggca
    14761 tcggcgttcg cgccatgctg gtggccttta atacttggct gttgctgaaa gcgtctttag
    14821 aaacggcgct aacagcgggt ttgtggaatg ccggatcgct caatggcctg acgtgggcaa
    14881 aaacttcgcc ttccgcaccc atcatcatat tgatgctcat tgccgccgcc ttgctggtgc
    14941 ggcgaatgcg cttgctggag atgggcgatg ataccgcctg cgcgctgggc gtcagcgtcg
    15001 agcgttcgcg tctgttaatg atgctggttg cagtggtgct taccgctgcg gcaacggcgc
    15061 ttgccgggcc gatttccttt attgctttag tcgcgccgca cattgcccga cgcattagcg
    15121 gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg agcgctgtta ctgctggcgg
    15181 ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc gtcgttaccg
    15241 tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc aaaaaatgac
    15301 cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg ggatatggca aatataccgt
    15361 tgcggaaaat ctgactgtag aaattcctga tggtcacttc acggcaatta tcggaccaaa
    15421 tggctgtggt aaatccacgt tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg
    15481 gcatgtctgg ctggatggcg agcacattca acattacgcc agtaaagaag ttgcacgccg
    15541 gattggtctg ttggcgcaaa atgctaccac gccgggcgat atcaccgtgc aggagctggt
    15601 ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc tggcgcaaag aggatgaaga
    15661 agcggtaacg aaagcgatgc aggccacgag aataactcat ctggcagatc aaagcgtgga
    15721 taccctttct ggcggacaac gccagcgagc gtggatcgcg atggtgctag cccaggaaac
    15781 ggcaattatg ctgctcgacg aacccacgac ctggctggat atcagtcatc agattgatct
    15841 actggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg cggtgctgca
    15901 cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg aagggaaaat
    15961 tgttgctcag ggagcgccga aggagattgt cactgctgaa ctgattgagc gcatttatgg
    16021 tctgcgatgc atgatcattg acgatccggt agccggaacg ccgcttgtgg tgccgctcgg
    16081 acgaacggca ccttcaaccg caaatagtta aactaagtgg tctgccatca tggcatcctg
    16141 ttttctggat gccatcgcat agcgcaataa cacgctacca caagccacca tcccgccgat
    16201 cagcgcggaa aggatcacaa taatcgcctt acccggaccg tcttttttca ctggcaatga
    16261 cgggcttaac tgatatttaa acggcgtaaa attcacatcg ttgacatttg cttttgttaa
    16321 ttgctcgaca agatactgcc gattacgtaa ttcaccgttc agttccgcaa cgtcagtaac
    16381 cgctttttca atttccagtt tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc
    16441 ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc ctgccgcgtt
    16501 ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt
    16561 aatgcgatcc tgagccagtt tttctttttc aaactgggtt ttgatctcca gtttattacg
    16621 gacgttttct atcgactctt tcaccaccaa cgcagagata taatcgatat acccgctcaa
    16681 aacggtctgc gcctctttac tggttggcgc ggtaaaactt agcgtccagg aggtatacag
    16741 tgacggttca tcttttttct tactggcatt gtcatcaacc gctttcattt tttcgctcaa
    16801 tgcaacaata gcgcgatgca aatccagttc atcgattttc gcctctttta attggtccat
    16861 cacataaggt gatgaacgca ggtactcttc cagcaagcta acagactgaa acttcttgat
    16921 aaacaggtta aatgcaccgc cacggtcgat attaacatcg agatcaagaa cgcggagttt
    16981 ggtaaaggtt ttctccaggt cctgccactg aatggcttcg gcgggagtaa taacggctga
    17041 gctggtccat ttttgcggca ggatgaaaga gatcagtaaa cctgcgcagg caaatgcaaa
    17101 aacgaccgcc atgaccgttt ttttggcccg ccataaaact tcgattagat taagtaaatc
    17161 aatttcatta ttactgggcg acgccagagg atactcagaa aaatgagcgt cacttccctg
    17221 tttaatattc aatgatgaca tggggtataa cctgaaaatg agtccaatag gggaaaatct
    17281 ccacggcgct cactttacca gttggtgaga attatccgaa gctgaagttt gtaaattcct
    17341 tccactgaat gcaatattca ctctatttaa gagaaatatt catcaaacct ataaatcaaa
    17401 caaataaaaa acccataatt attttctgta attatgggtt tatagataat attaatttac
    17461 ctgtttagcg ttgcgcgaat aatcggccca atttttacaa acgcccctgg agagataata
    17521 tccacatgcg cacaatcctg acgatagata tccagctccg caatccacgg cgaccaggcg
    17581 cgttcaggac tcataccttc ctgaagcgtg cgttcagcaa caaacaacgt cgctttaccg
    17641 tcaaacggta cgctatgagc agtcgtcagc aggcgcacag catcagcgta gttgccttca
    17701 atggtggtaa acaactccgt tgaagtactt ccctgctgtg ccgccaggaa ggcctcgcgc
    17761 tcgcggttaa tctccgccag cacttccggg tccagaccat tagcttcttt ttcctgccag
    17821 ttttgcgttt ctggcggcca ggtatccagc aagccaagaa atgccacctg ttcgccacgg
    17881 gcacgcagtc gcgccgcaat accctgcgcc agcgtaccgc caagggaata ccccagcagg
    17941 taataagggc cgtgcggttg ttgctcaagt aacgttgcca gatgcgcttc gcagacttca
    18001 tccaggtttg tcgctgtctg catgggtcca tgagggcgcg gcgactgaat gccaataatc
    18061 gaccattgtg gatcgagata acgcgagagt acgctaaact gccaggcaaa accggacgcc
    18121 ggatggaaac aaaacagcgt cgggccatta ccttcacgca acggcagaat ggtttcgaat
    18181 cccatgcgcc gggagctgtc ctcttcacca tcaataatcg ttgccagttt ggcgacggtt
    18241 gacgcgacca tcacctgccc cggcgtcacc tgacgagcaa actgtcgact taactgcgct
    18301 gccagtttca ttgccagtag cgaatgaccg cccagcgcaa agaaatcagc atcggcatcc
    18361 tgcacgtcac aacccagcaa cgacgcgaat gccgcggcga taatcgtttc actgcccgct
    18421 ttcggtgcac gtcccgacgc ttgtgtcttc agttcaggca acggtaaggc tttgcgatcc
    18481 agcttgccgt tggcgctaag aggtaactgt ggaagttgca gcagaaccac cggtaccata
    18541 tgtggtggca atgtttcacg aagctgcgcc tgcaatgcgc tggtatccaa cggcaggccc
    18601 gattgcgaca ccagatagcc caccaactga cgcgcatcac caccagtggc tgccgcctgg
    18661 ttaatcacac aggcgtgggt aacggcttgt tcgacatccg gcagcgcctg catcacgcga
    18721 tcgatttcgc ccagttcgat acgctgcccg cgaattttta gctgatcgtc actgcgcccg
    18781 aggtactcca ccgcgccgtt atccagccag cgggcaacgt ctccggtacg gtacatccgt
    18841 tcgccaggag caaaaggatc ggcaataaag cggctggcgg tcagatcggg gcgtccgaga
    18901 tagccctgcg ccagttgaat gccggtaaga tagagatcac ccgccacacc cggcggcacc
    18961 ggatgcatca tcgcatcaag aatacgcagc cccatgttcc acaccggata accaatcggc
    19021 acactgctgc cgcgcacctg tgccagttcc tcgccaaaag ccggatacca gctgacatct
    19081 accgccgctt ccgtcgggcc atacagatta tgtaacggcg caccggttaa ctgttgccat
    19141 tcacggcata aatcagccgg taaggcttca ccactacaga aaacctgttt caacgtcgcg
    19201 caactctggc gagcggtttg cggagtcagc gaggcaacaa atgccgccag catcgacggc
    19261 acaaagtgcg tggtcgttac gccatattcg gcaaagaatt gctgcatagc gagcgggtca
    19321 cggtgcgctt ccggttcagc catcaccagt tttgcccctg cgataaacgg ccagaaaaac
    19381 tcccacaccg agacatcaaa actgcacggc gttttttggg caacgacatc ttcgcctgta
    19441 agcgggtagt gattttgcat ccataacagg cggttaacga tagccgtctg cccgaccatt
    19501 acccctttcg gcctgccggt ggagccagag gtaaagatga tataagccgt gtggtgcggt
    19561 tgtgaaagtt gcagcggcgc actgccctgg ggggtaagcg gggcgttata gcaaaggctt
    19621 gttaaattgg gaacatcgct aaagcgcggc agttgatcgt cggtggtaat taacagcgac
    19681 ggacgcgcat cttccagcat cattttcagg cgatcgtccg gatagccggt atccagcggt
    19741 agccaggccg cgccagcttc aactattgca tggagtgcca gggtcaaaaa gaccgagcgc
    19801 ggtagtgcca ccgccacgct gtcccctggt ttaacgccgc gctcacgcag cagattcgcc
    19861 agcgccacca cctgctcgcg catttcccga tagctgaaca ggtaacgcgc atctgccagc
    19921 gccggagcat ccggtgtttt tgccgcttgt tctgccacca gcgcgctcag cgtggtttct
    19981 ggaatctcaa cctgagtggc gttgatctgc gccagctgcg catactcacc tggcagcata
    20041 atatcgacat cgccgcacaa cagcgccgga tccgcggcaa actgggcaat cagcattttc
    20101 aggcgttcag catgctggat taacgttggc tcatcgtaac gctgtttatt ggcgaggatc
    20161 tcaatactca aatcaccgtg ttcatccggg aacagggcca gttcaaggtc attaaccgga
    20221 ccggttgcca gggtatgggt ttgcgcctga acaccaggaa tatccagttg gtaatcaaat
    20281 accttaatat tgagtaccgg accaaacagc ggttcatcac ctgccgctcg cccgctgtca
    20341 cggacaattt gttcggcatc gtaacgttga tggcgacgca tttttttcag ttgagccgcc
    20401 agtcgggttg ccagttccgg cagcgtttct tgcgccgcaa tgtgaatacc caacggtaaa
    20461 acgttgagca cgggtccgct agccgtcagc gccgccgagc ccagtcgacg cataaagata
    20521 aatccggcgg catagtccat gcgattgcac aatcgcccca gccacaaggc tgccagcgca
    20581 agggctaaat cggtacgctg cacacctgaa agttgcgtag ccagctggcg gaattccccg
    20641 tcggtaaatt ccagtttcag gcgcagaata tctgccgaag cgctgcgccc cggtaaaggt
    20701 gccggagaaa gtgacgcggg cggcggcagt tgacgacgct gttctgccca gaatgccgca
    20761 tcacgctgcc aggcttcgct ttcgcggtat tgctggtact cttccactac atcagcgaaa
    20821 ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagcc atgtgcagta aatattggcg
    20881 atctggcgag taatggccgg gaaactgaag ccatcgacca gcaaatggtg ataacgctga
    20941 taccagtacc agcggttatc cgcaacatgt atcagctgat gaaagaccag tggtttaccg
    21001 ctatcgacgc gcaaatcttg ttgcaaatcc gccagcatta atgcctgcgc agtaccgtgc
    21061 ggatcaatat tggttcgcag gtcgataatt tctggctgtt cgaatatcag cgcatcatcg
    21121 acccactgcc agacttcgcc gttatcttcc gtaaaacgca tccgcaacgt atctgcttgc
    21181 gctagtccgg caaccaccgc gcgggccagt aatggcgcat caacatctcc agttaactca
    21241 acgtaatgcg ccacgctcca ggcggagggt aaatccgaca gtttttctgc catccagatg
    21301 ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg tgcctcctgc aactgggtga
    21361 aattggccgg tgtcagagta cgccagtgga cttccagcca ctgctggcag gacgcctgtg
    21421 actgcggctg acacacaatg tcccagcctg ccgataagag gcattgttgc ggccacagac
    21481 tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc ctgcggatca tcgaagggat
    21541 tactgaatgc catactcaac tcctgtcatg gaaaagtggt tgccagaggt cgattaaccc
    21601 ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg ccgtcaacct gacgccagaa
    21661 aatggattct tttatagggt gcaattgcgc atacagcgcc tgattggccc gcatgatcat
    21721 cggctcgcga atacccgctt ccagcacaat gcgcagacct tcggcgctaa cttcaccagc
    21781 ttttagcttt tcaagtaaca cgccctcttg ctgcccgtcc cgatgcggcc accagtacga
    21841 tcctgactgg cttaatacac agccaaagcg ttcaggccag tgcagtccgg catacagcgc
    21901 ggacagccca ccaaaactct gcccggcgac cacggtgcga tcggcgcgat cgctaaaagg
    21961 ggcaatagct ttcaccaggg gtaataactc ttgctgtact gccagccaga aatccgcatt
    22021 acacggcagt tcgtgggcgc ggtgcgtggt gtcgatagcg tcgatcaaca catacacggc
    22081 gggaggaagt tgctgacgat gggtcagcga agtcagcaca ggccagacgg gcatactttg
    22141 cgcccaaaat tcgccatcga gcaaaactgc cagcggacgt tcttctgctg ttacatcgcc
    22201 ggtggtaaaa atccatacac gacgtgaatt tttcaaccgt tcacttttcc agataatttc
    22261 tttggctgct atttctggca cttgcggaca atcccatccc ggttgcagag gcgcttgcgg
    22321 catttcgagt gcagaaacag cgtgccctcg cccgcctttc cagctttgtg gattcagcgg
    22381 atcggctatt gcctggggta atagttttcg ccagccttca cgtaattcga ggcgatcggg
    22441 ggatggtgca gaaaaaatgt catcgcgttc ggtgggaata aagcagtagc tgccgcgcca
    22501 gttggcattg agttgtgtcg tccactgcca gacatcagta cctgcaattc gctgcatcga
    22561 ctggggctgg ctgttctgat gatgatcggt cacaccagtg atgtagatcc atacgcgctt
    22621 tatcgtcgag tattcttcag aaccttgggg atcacgccac cagaaagtga cctcaaacat
    22681 ttcgtcattc agacgctgcc attccgggcc atgtttcgac tgccaccagc tctcacttcc
    22741 tacttttaac gccgtcacac cataacccca tgtttactgt gcaatttttc attgattgca
    22801 gaaatatatt gataatatta ttgataacca tttgcatttg caatagcgta atggcgcgcc
    22861 gtgggaagcg cggacattta ttaaccaact gcactgcgtg tctttcagga tcaaaggttt
    22921 tcgcggtagc gggatgcgtc gtgttgagga cgaccatgcc cgacagttgc aattcgtggc
    22981 aaaaatgcag gaataaaaca atgaacaaga agattcattc cctggccttg ttggtcaatc
    23041 tggggattta tggggtagcg caggcacaag agccgaccga tactcctgtt tcacatgacg
    23101 ataccattgt cgttaccgcc gccgagcaga acttacaggc gcctggcgtt tcgaccatca
    23161 ccgcagatga aatccgcaaa aaccccgttg cccgcgatgt gtcggagatc atccgtacca
    23221 tgccaggcgt taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga
    23281 ttgatattcg cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa
    23341 gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatacct
    23401 cctgggtgcc acctgaaatg attgaacgta ttgaagttct gcgtggtccg gcagctgcgc
    23461 gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggtg
    23521 agtggcacgg ctcctgggac gcttatttca atgcaccaga acataaagag gaaggtgcca
    23581 ctaaacgcac caacttcagc ctgaccggtc cgctgggcga cgaattcagc ttccgcttgt
    23641 atggcaacct cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg
    23701 cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg cgaaggggtg atcaacaaag
    23761 atattaatgg cgtggtgcgc tgggatttcg cgccattgca atcgctggaa ctggaagcag
    23821 gttacagccg ccagggcaac ctgtatgcgg gcgacaccca gaacaccaac tccgattcct
    23881 atacccgctc gaaatatggc gatgaaacca accgtctgta tcgccagaac tactcgctga
    23941 cctggaacgg cggctgggat aacggagtga ccaccagcaa ctgggtgcag tatgaacaca
    24001 cccgtaactc gcgtattccg gaaggtctgg caggcggtac cgaagggaaa tttaacgaaa
    24061 aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agcgaagtta
    24121 acctgccgat tgatttcctc gttaaccaaa cgctgacgct gggtacggag tggaatcagc
    24181 aacggatgaa ggacttgagt tccaataccc aggcgctgac cggggcgaac accggcggcg
    24241 ctattgatgg tgtgagtgcc accgaccgta gcccgtattc aaaagcagaa attttctcgc
    24301 tgtttgccga aaacaacatg gagctgactg acagcaccat cgtaacgccg gggctgcgtt
    24361 tcgatcatca cagtattgtc ggcaataact ggagcccggc gctgaacata tcgcaaggtt
    24421 taggcgatga cttcacgctc aaaatgggca ttgcccgtgc ttataaagca ccgagcctgt
    24481 accagactaa cccgaactac attctctaca gtaaaggtca gggctgctat gccagcgcgg
    24541 gcggctgcta tcagcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga
    24601 ttggtctgga gttcaaacgc gacggctggc tggcgggcgt cacctggttc cgtaacgatt
    24661 atcgcaataa gattgaagcg ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc
    24721 tctatcagtg ggataacgtg ccgaaagcag tggttgaagg tctggaagga tcgttaaacg
    24781 taccagttag cgaaatggtg atgtggacca ataacatcac ttatatgctg aagagtgaaa
    24841 acaaaaccac gggcgaccgt ttgtcgatca tcccggagta cacgttgaac tcaacgctga
    24901 gctggcaggc gcgggaagat ttgtcgatgc aaacgacctt cacctggtac ggtaagcagc
    24961 agccgaaaaa atacaactac aaaggtcagc cagcggttgg cccggaaacc aaagaaatca
    25021 gtccttacag cattgtgggt ctgagcgcga cctgggatgt gacgaagaat gtcagtctga
    25081 ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca
    25141 cgggcgatct ggcaggggcc aactatatcg cgggtgccgg ggcgtatacc tataacgagc
    25201 cgggacgtac gtggtacatg agcgtaaaca ctcacttctg atgctaacgt cagattgttg
    25261 acaaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat
    25321 cttgccaatt caatatattg caggaccatg taggcctgat aagcgtagcg catcaggcag
    25381 ttttgcgttt gtcatcagtc tcgaatatgg tcgatatgaa aactacgcat acctccctcc
    25441 cctttgccgg acatacgctg cattttgttg agttcgatcc ggcgaatttt tgtgagcagg
    25501 atttactctg gctgccgcac tacgcacaat tgcaacacgc tggacgtaaa cgtaaaacag
    25561 agcatttagc cggacggatc gctgctgttt atgccttacg ggaatatggc tataaatgtg
    25621 tgcccgcaat cggcgagcta cgccaacctg tctggcctgc ggaggtatac ggcagtatta
    25681 gccactgtgg gactacggca ttagccgtgg tatctcgtca accgattggc attgatatag
    25741 aagaaatttt ttctgtacaa accgcaagag aattgacaga caatattatt acaccagcag
    25801 aacacgagcg actcgcggac tgtggtttag ccttttctct ggcgctgaca ctggcatttt
    25861 ccgccaaaga gagcgcattt aaggcatcgg agagacaaac tgaagcaggt tttctggatt
    25921 atcagataat tagctggaat aaacagcagg tcatcattca tcgtgagaat gagatgtttg
    25981 ctgtgcactg gcagataaaa gaaaaaatag tcataactct gtgccaacac gattaattga
    26041 caacatctgg tacgattcgc ccgcagccat cactgactgc gggcgaaagt gtaaagcagg
    26101 tgccttacca ctctgacctg acaaccggat atgcagggat tgctccccgc acaacggcta
    26161 cttcttcggt tcgtaagcga gaacagcctt aaactcaata ttacgttcct ttacggtgaa
    26221 ctcacacagc gagtctccga ccagaagcgt aaatcccagc accgtcaaac acagcactat
    26281 gactgccaca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac
    26341 taccatccga gttgttgagg ttcggagtgg cagccccagg attgatagaa atatctcctg
    26401 gggcttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc
    26461 gttatcttac ccttcctcag caaaatatct gtttatttat acagtaagtc ctgtttcgga
    26521 cttctgttgg caacactcgt tattgctcgt aaaacgaaat cgcccgcagc catcactgac
    26581 cgcgggcgaa agtgtaaagc aggtgcccta ccatcctgac ctgacaaccg gatatgcggg
    26641 gattactccc cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca
    26701 atattacgtt ccttcaccgt aaactcacac agcgagtctc cgaccagaag cgtaaatccc
    26761 agcaccgtca aacacagcac tatgactgcc gcaagggcat atttcgtcag catgtttata
    26821 ttgcctccat gcgaagaagg gactaccatc caaattgttc gggtttggag tggcagcccc
    26881 agggttgata gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa
    26941 ccggaaagct tcaggcaccc gccgttatct tacccttcct caacaaaatg gctgtttatt
    27001 tatacagtaa atcctgtttc ggacttctca ctcctcattt aatcaactga agctgagccc
    27061 gttttttctg catgcctcaa tcgtcaaaat tcttcgccat cagccagctc tgccataact
    27121 tgccacattg aaaaagtagc caaattgctt cagtcaccat catcaaaaaa agaaacattc
    27181 cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa aacgccaaca
    27241 taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac cctcggtatt
    27301 gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga ctcttcaatg
    27361 ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga tatcaccgaa
    27421 agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcaattt
    27481 tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga aatttgcggc
    27541 ggtggcacac acccgtttca gaaatggcag cgtcaggagg tatgcgataa cgagcgctat
    27601 caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat
    27661 gtccatgttg gctgcgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg
    27721 tttgtgccgc actttatcgc cctttccgcc gcgtcgccat atatgcaagg aacggatacg
    27781 cgttttgcct cctcacgacc gaatattttt tccgcctttc ccgataatgg cccgatgccg
    27841 tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtttgagtta caccacgatg
    27901 atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt tggcacggtg
    27961 gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat ggcgggatta
    28021 attcaggcta ccgcccactg gttactgaca gaacgcccgt tcaaacatca ggagaaagat
    28081 tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atgggctgga aggcgtcatt
    28141 accgatccgc acactggcga tcgtcgaccg ctaacggaag acaccttgcg attgctggaa
    28201 aaaatcgccc cttccgcaca taaaattggt gcatcgagcg cgattgaggc cctgcatcgc
    28261 caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc cgatggtggc
    28321 tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccgatgacta agtagggaat
    28381 tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac aatgttatga
    28441 aacacccttt agaaaccttg actaccgcag caggcatttt gctgatggct ttcctctctt
    28501 gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg gtgaccatgt
    28561 tccatctgat ggatcttagc tagctttaca ctttattgtt ttgtctgtgg tttttagtgc
    28621 tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc tcgctggcgg
    28681 attaaacgtc gattatcgcc gtacttcgcg gcatactttg cttattctcc ttcgcctaaa
    28741 ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctggc ccttgagtta
    28801 ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc
    28861 aaaattttta tgctgtcgtc ggagctgcgc ggcgtcccct ttatcaatct gaagtccgat
    28921 ccacaaaaat ccctgttaaa tcagcagata tacccgagca ttaagccagg gtatcacatg
    28981 aataaaaagc actggatttc ggtgtatccc ggcgaggaaa tctccgaagc gttacttcgc
    29041 gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa
    29101 agagtgcgtc caggctaaag cggaaatcta tagcgcattt ttctcgctta ccatttctcg
    29161 ttgaaccttg taatctgctg gcacgcaaaa ttactttcac atggagtctt tatggatatc
    29221 atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagtaa aaaacttacc
    29281 ccggaacagg ccgagcagat caaaactctc ctgcaataca gcccatccag caccaactcc
    29341 cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc
    29401 gctgccggta attatgtgtt caacgaacgt aaaatacttg atgcctcgca cgtcgtggtg
    29461 ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat
    29521 gctgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc
    29581 ttcgctgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt
    29641 tatctcaacg tcggtaactt cctgctcggc gtggcggctc tgggtctgga cgcggtaccc
    29701 atcgaaggtt ttgacgccgc catcctcgat gcagaatttg gtctgaaaga gaaaggctac
    29761 accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa cgctacgctg
    29821 ccgaaatctc gtctgccgca aaacatcacc ttaaccgaag tgtaattctc tcttgccggg
    29881 catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc
    29941 atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag
    30001 atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc ggtattattt
    30061 ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg accttcgaaa
    30121 aacgagccat cgccagttca cggctttggt tgcaaatcat aacccagaat aaactcttcc
    30181 accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta ttctggctgc
    30241 aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt
    30301 atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg cagaaattcc
    30361 cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg atcggcgcga
    30421 tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg attctgatca
    30481 gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg attcttggtc
    30541 tggtggccgg tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaga
    30601 tgccgaaata cggcgcagat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc
    30661 gtaactggga caataccatt accactattc ccacctggtc tctggtttct gactccttta
    30721 aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt atcagtattg
    30781 atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt
    30841 tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag
    30901 gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct
    30961 atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc ttaatggtac
    31021 gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt actaacaccg
    31081 tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg
    31141 tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg
    31201 cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga gagcggacat
    31261 cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa atgccacaaa
    31321 caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat ccatcgtgca
    31381 catcagcagc aaaatcatgc cgaggaaggc aatgcagaga tagtttccga acggatagag
    31441 cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gctgcacgaa aacgcagatg
    31501 cgccagacag atcataatcc agttcaacag cagcgttgct accaccagcg ccatcagcag
    31561 accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc
    31621 ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca aaaacttcgg
    31681 cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt
    31741 atacactgac agcgatgcta ccagaatgac gaagttcagc gcagaagcta ccacgttgct
    31801 gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt ccacccacgg
    31861 atagagcgcc agtaaaacca ccagtgaacc gatgtaaaac agcaggatgc gatacaccac
    31921 ctgatttacc gcttttggaa tgcttttatc cggatcgcgc gcttcagcgg cagtaatccc
    31981 aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagcccatt
    32041 ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg ctttctcgcc
    32101 gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga ttgccagcac
    32161 tttaatcaac gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac
    32221 ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat ccgggaacca
    32281 gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac
    32341 ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg
    32401 ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc gcataatcag
    32461 gaaagcgatg atcccggcga cgccgtaacc cagcaataca gccggacccg ccatctgaat
    32521 cgccggacca atgccaagaa acagaccagt accaattgcg ccacccagcg caatcagttg
    32581 aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc
    32641 cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac
    32701 cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cggggtatcc
    32761 tgcttttatt tccgtacccg atgtcggtgc agccacatca gcttatacgc cgccgggata
    32821 ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc ggcaatccgg
    32881 ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc ggcgataatc
    32941 accgccaccg tcatcgcttt cgggcgcacg cgcagtaccg cgccgtgata caacgcctca
    33001 tccagcttct gctcactgaa tgtttgcgga ttattcaacg acggctcggc ctctatggcg
    33061 tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc aagggcaata
    33121 aaaccggtgc ccgttgccac agaaagatga aagcccatcc accacagcaa ccagatgccg
    33181 cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac ccgacggaat
    33241 gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag tttcagctta
    33301 tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt gcccggtttt
    33361 aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac catgtcacga
    33421 tcgcgggcat caatataaat ccagctcgtc gggcgcgcat tctcggtttt cagcatcgac
    33481 ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg cttcatcggc
    33541 gtcaggatcg gtagctggcg cagtgcctgc gggctatcgc gccagctttg cggataacgc
    33601 agattaatgg gataacgggc aatcccttcc accgtttcgc caaccatcgc cccgcccacc
    33661 gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgcgc ggctttttca
    33721 cggttaatct caacgttgat atagcgccca ccttccagac gctcggctag agcagaagcc
    33781 acgcttggca ccgttcgcgc tacttcttca atttgctcag ccatcgcgtc gatatccgcc
    33841 agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga gagcatatcg
    33901 atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagccg cacggtgtta
    33961 tccagttcct caatgatttt gtccatcgtc atgccaggcc gccactgatc ctgcggctta
    34021 agctggatgg tcgtttcaac catctcgagc ggagcagaat cggtggcggt ttccgctttc
    34081 ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt gtcggttttt
    34141 tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg catatacaac
    34201 aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag cggccagaga
    34261 accgtcagca ccgaaagcgc tgccaccaac agcgtggttt tcggccagtg cagtactttc
    34321 agcaatagag gatgataaac acgaatcaaa aagcgattga gcgggttact gctttccggc
    34381 ggaattttgc cacggatcca gtaccccatc aggataggga tcactacgat cgccagcagc
    34441 gccgcgcccg ccatcgcata ggttttagtg aacgccaacg ggccaaacag acgcccttcc
    34501 tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag actgataaat
    34561 agcgccggac ccacttcaac agatgcatcg gtgatcacct gccagcgcgt tttattatcc
    34621 agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgtgc attttcgatc
    34681 atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccgcc caacgacata
    34741 atattggcat tcagtccctg gaagtgcatg acaataaaag caatacacaa ccccagcggc
    34801 aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc acagactacc
    34861 gccaccacga taaactcttc cagcaacttg ccgctgaggt tgtcgatggc gcggtcaatg
    34921 agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact acttttcagc
    34981 gtttccagtt tgtccttcac ggaggcgatc acttctcggg cgtttttgcc ggagcgcagg
    35041 atcaccaccc cgcccgccac ttcgccttcg ccattcagtt cggcaatgcc ccggcgcatc
    35101 tccgggccaa tctggacctt cgcaacatca cgcagataaa cgggcacgcc attttctctg
    35161 gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct ggcgcgcacc
    35221 atatattccg cttccgccag ttcgatcgac gaaccgcccg cttcctggtt tgaagcatcc
    35281 agcgcgcttt ttacttcggc tagactgatg ccatattgcg ccaggcgctg gggatcgata
    35341 accacctgat actctttcac cacgccgccc accgacgcca cttccgcaac gtcagggatg
    35401 gtttttagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc cagatcgtgc
    35461 ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt ggcatctggt
    35521 cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag gtactccagc
    35581 acccgcgaac gcgcccagta cggatcggta ccatcttcga aaatgacata cacataagag
    35641 tctccaaact gcgagaaacc gcgcacagtc ttcgcgccag gcaccgacaa catggtggtg
    35701 gttagcggat aagtcacctg attttcaacg atttgcggtg cctgaccggg atagctggtt
    35761 ttaataatca cctgcacatc ggagagatcc ggcagcgcat ccactggcgt gttaatgatg
    35821 gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa acggttcgcc
    35881 accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca ttggtagcac
    35941 tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca atcaggaaca
    36001 ggccgctgga aaccaccttt tcaccttccg ccagaccaga gtgtaacgcg gtgacgcctt
    36061 gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg tccgtcggca tcaacggtaa
    36121 tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc agcatcggtt
    36181 cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc tcgtcggcgt
    36241 tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca ggtagcagcg
    36301 tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagc gtaaactgcg
    36361 aggcatcttt caccagccag gcgatagact ccgggatcgc agcagtgacc cacaccgggt
    36421 ccataccctg aattttcgct accacgttat ctttggcgat attcattccc gcgcgcagat
    36481 caaacgcggt gatcacgcca tcaatgggtg ctttgagcgt aaagcgagtc tggatttttt
    36541 gcgtggcgat caggcggcga atatccgcct ccggcattcc cgccagtcgc agccgctcaa
    36601 gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa tactcactct
    36661 gcgcttccac ccagtcagga atggtcaggt cgagaagcgg tgtgcccttt tgtactttat
    36721 cgcccacggt aagcggatac accttgtcga taaacccggc agcgcgagcc tgcacaatgg
    36781 catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggcaaaa gtcagcggtc
    36841 cgcgcgtgac ggtagccgtt ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca
    36901 caccagacgc agaactctct tcatcggcat atttcggcac cagatccata tccataaacg
    36961 gcgatttacc tggtttatcg aaccgcgtat tggggtacat cgggtcgtac cagaataaga
    37021 ttttacgttc tgcggtcgac gttttttctg cgggtggttc agcctttgca aaccaggtaa
    37081 aacctgccgc agaaataata ccgcccgcga tcatgctgcc gataataagc gcgatttttt
    37141 tcatctcatt aaacctgggt tactggctga ctttaatatc ctgtaataaa gaaaggttgc
    37201 cctgctggac aaaattaaac gccactttgt cgccggtttt aatttcactc attttcgtct
    37261 gcggggtgat ggtaaagcgc atagtcatct ccggccagtt cacggcagca atcggatcgt
    37321 gatggatggt gatttttttg ctttccagat caataccctt taccacgcca gtggcgctaa
    37381 taacctgcgg ttgtacttcg ctcatggttt catgatgatg ttcgttagcc tgggcattaa
    37441 agccaataac ggtaaacaga ctgaacattg cgacttgcag tgcttttttc atttattctc
    37501 tcctggagtt aaaaattatt gctgccaacc gccccctaag gcggtataca aagaaatttc
    37561 gttaacctga cgggcataat tcaggtcgag taaagtttgt cgggttgcaa ataaagaacg
    37621 ctcggcatcc agcacttcca gataacttac cgcgccgtgc tgatataatg cccgcgcccg
    37681 ttgcaaagta atttgcagcg acgccagata acgctgctgg gcgctgattt gatcgttcag
    37741 gctttgacgt aatgccagcg catctgccac ttctttaaag gcgttctgga ttttctgttc
    37801 ataattcact accgactgct gctggcgaat ttcggcgata tccagattgg cctggttgcg
    37861 tccggcatta aaaatgggga tctcaatttt aggaataaaa ttccacatcc cgctgctagc
    37921 gttaaataat gaagacagat cgctgctggc ggttgatatt ccgctggtca ggctgataga
    37981 cgggaaaaat gccgcacgcg cagcaccaat attagcatta gccgccatta acgcgtgttc
    38041 agcttccata atatccgggc gctgcaataa gatttgcgac gacaagcccg ccggtaattt
    38101 aacgctttgc aggctgccgc tgtttactgt ctgcgcttgc ggcagtttgc cgtagcttcc
    38161 caataacagt tgcaatgcat tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc
    38221 actgcgggta ctttctatca ccccgcgagc ctgttccagc gccagaacat tgctgctacc
    38281 ggtcaacagt tgtttttcga caaacgcata tgactgctga taattacgca gcgtttcttc
    38341 ggctatttgc aattgcgcat acgccagttg ctgattgaaa tagctttgcg cgacattaga
    38401 aaccagcaga atatgcaccg cgcgctgagc ttcctcagtg gctaaataat tttgtcgctc
    38461 ggcttcgctc atgttcttta agcgaccgaa aaaatcgagg tcaaagctgg cgttaaggcc
    38521 ggtcgaaaac tcccgtgtcg tggcggtatt ccctttaaga ttaccgctcc agctgccgct
    38581 gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc
    38641 ctcctgcact ttcagcgtcg ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat
    38701 cagcgtcttc acctgattat caacaaaaaa ggtgcgccag cccacgttct ggtagttatc
    38761 tgctgcgtta accaagccgt tctggctgag tgagaactgc tgcggcacgg gcattgccgt
    38821 acgctgataa tccggtgcca gtgaacaacc ggttagcgca agggccacac aaaatggcag
    38881 aagtttacaa ggagacatag gctcataatt tctggtgatt ttataccgcc aactttactc
    38941 gccaggctct gattttccgg tgacaggaaa atgacaaaat tgtcattttg ccaataagcg
    39001 attgccatct gatcccgcta ctctagaatt gcccgggcaa catgcggagg aaaaatgaaa
    39061 ctgttgattg tcgaagatga aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa
    39121 gccggttttg tggtcgattt ggccgacaac gggctgaatg gctaccatct ggcgatgacc
    39181 ggtgattatg acctgataat cctcgatatt atgctgccgg acgtgaacgg ctgggatatc
    39241 gtgcgcatgt tgcgctccgc caataaaggg atgccgattc tgttgcttac cgcgcttggc
    39301 accattgaac atcgcgtcaa ggggctggag ttgggggccg atgactatct ggtgaagccg
    39361 ttcgcttttg ctgaactgct ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg
    39421 attatcgaaa gtcagtttca ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc
    39481 acccgcagcg gcacgcgcat cactctgacc agtaaagagt ttactctgct ggagtttttt
    39541 ctccgccatc agggcgaagt gctgccccgc tcgcttatcg cctcgcaggt gtgggacatg
    39601 aatttcgaca gcgacactaa cgccattgat gtggcggtga agcggctacg cggcaaaatc
    39661 gacaacgact ttgagccgaa gctgattcag accgtgcgcg gcgtgggcta tatgcttgag
    39721 gtgccggatg gtcagtaagc catttcagcg cccgttttcg ctggcaaccc gtctgacctt
    39781 ttttatcagc ctggccacca tcgcggcgtt tttcgccttt gcatggatca tgatccactc
    39841 agtgaaagtg cattttgccg agcaggatat taatgattta aaagaaatta gcgccaccct
    39901 tgaacgggta cttaatcatc ctgacgagac gcaagcccga cgcttaatga cgctggaaga
    39961 tatcgtcagt ggttattcca acgtgttgat ttccctggca gatagccacg gcaaaacggt
    40021 gtatcactcc cccggtgcgc cggatatccg cgagtttacg cgtgacgcca tacccgataa
    40081 agacgctcag ggtggcgagg tgtatctcct ttccggcccg acgattatga tgccaggcca
    40141 cggtcacggg catatggaac acagcaactg gcggatgatt aacttgccgg ttggcccgtt
    40201 ggtggacggc aaaccgattt atacgctcta catcgcgctt tcgatcgatt ttcatcttca
    40261 ttacataaat gatttgatga ataaacttat tatgaccgca tctgtcatca gcatcctgat
    40321 cgtctttatc gtgctattgg cggtacataa aggtcacgcg ccgatccgca gcgtcagccg
    40381 tcaaatccag aatatcacct cgaaagatct cgacgttcgc cttgacccgc agaccgagcc
    40441 cattgagctg gaacagctgg tactgtcgtt caaccatatg atcgagcgta ttgaggatgt
    40501 ctttacccgc cagtccaatt tctcagcgga tatcgctcac gaaattcgca cgccgattac
    40561 caatctcatt acgcaaacgg aaatcgccct cagccagtct cgcagccaga aggagctgga
    40621 agatgtgctc tactctaatc tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat
    40681 gctgtttctc gctcaggccg ataacaacca gctaatcccc gaaaagaaaa tgctcaacct
    40741 ggcggatgaa gtcagcaaag tgttcgattt tttcgaggcg ttagcggaag atcgcggcgt
    40801 ggagttgcga tttgttggcg acaaatgtca ggttgcgggc gatccgctga tgctgcgtcg
    40861 ggcgttaagc aacctgcttt ctaatgccct gcgttatacg ccaaccagag agacaattgt
    40921 agtgcgctgt cagacggtcg atcaccaggt gcaagttacc gtcgaaaacc ccggtacgcc
    40981 cattgcgccc gagcacttac cgcgattgtt tgaccgtttc taccgcgttg acccttcccg
    41041 ccagcgaaaa ggcgaaggta gcggcattgg gctggcgata gtgaaatcga ttgttgtcgc
    41101 gcataaaggc acggttgcag taacgtcaga tgcgcggggg acaaggtttg tgattatgct
    41161 gccggagaga gagtgattat taagttcaaa ttcagggcgt ggtgcatcac ctcctggtat
    41221 tgttgatcaa caaattagag aaaaacttat taaacatatg aaatatcgaa ggcttttatt
    41281 tttcaaagtt tcacgagatg tttgtatatg aaacttagtt aaatctgtgt ttcttccaat
    41341 tgtagatcac aatgcccttt ctaaattttc atcgttataa acaagccatc tatcatataa
    41401 agcaatgaat aatttaatcg cgattaatca tcattaattg ttatattatt cactcaatct
    41461 atttgtcaag tgaatttgat aaattcctaa tttaaaatat ccactattga gccagtattt
    41521 aacaacgtta agatgtgcaa tgtgcagatc atgttatcag ggtggagcgg tagcatatat
    41581 ttcttatgca atcattcatt ttaataatgt taattggatt atttattgct gtgaataaaa
    41641 atatattatg aatattggtt taactttgcg tttttgttac tcgttgtgat atgcgtacct
    41701 gtcctgaata acgccaagaa acaagaggtt gagcttgtga ttattttttt gactgaacag
    41761 acacccgttt ctgtaaagta atatttaaac aaaattctca cataaccata gcattttctg
    41821 ttccttccct aataacgtct gaagtcgtcc cgcttatcac attatccgca gttgtacggt
    41881 acggatcatt attcatttta ctggaggttg ctatgtcaac cggattacgt ttcacactgg
    41941 aagtggacgg cctgccgccg gacgcttttg cggtggtttc ctttcatctg aaccagtcac
    42001 tctcttcgct cttttccctc gatctctccc tggtcagcca gcagtttctc tcccttgaat
    42061 ttgcgcaggt gctggacaaa atggcctacc tgacgatatg gcagggcgat gaagtacagc
    42121 gccgggtgaa aggcgtggtg acctggtttg aactcgggga gaacgacaaa aaccagatgc
    42181 tgtacagcat gaaggtgcac ccgccgctgt ggcgtgccgg tctgcgccag aacttccgta
    42241 tcttccagaa cgaggacatc aaaagcatcc tcggcacgat gttgcaggaa aacggggtga
    42301 ccgaatggag tccgctgttc agcgagccgc atccttcccg tgagttttgt gtccagtacg
    42361 gtgagactga ttacgatttc ctgtgccgga tggcggcgga ggaaggcatc ttcttttatg
    42421 aggagcatgc ttacaaaagt accgaccaga gcctggtgct gtgcgacaca gtccgccatc
    42481 tgcccgaatc ttttgaaatc ccatggaacc cgaacacccg taccgaggtg agcaccctct
    42541 gcatcagcca gttccgctac agcgcacaaa tccgcccttc ttccgtggtg accaaagact
    42601 acacctttaa acgccccggc tgggccggac gttttgaaca ggaaggccag caccaggact
    42661 accagcgcac gcagtatgaa gtgtatgact accccggacg tttcaagggt gctcacgggc
    42721 agaactttgc ccgctggcag atggacggct ggcgaaacaa tgcagaaacc gcgcggggaa
    42781 tgagtcgctc gccggagata tggccgggac gacgaattgt gctgacgggg catccgcagg
    42841 cgaacctgaa ccgggaatgg caggtggtgg caagtgaact gcacggcgaa cagccacagg
    42901 cggtgccagg acggcaggga gcggggacgg cgctggagaa ccattttgcg gtgatcccgg
    42961 cagacagaac atggcgacca cagccgttgc tgaaaccgct ggtcgacggc ccgcagagcg
    43021 ctgtcgtgac aggaccggca ggcgaggaaa tcttctgcga cgaacatggt cgcgtgcggg
    43081 tgaagttcaa ctgggaccgt tataacccgg cagaccaaga cagttcgtgc tggatccgtg
    43141 tggcacaggc gtgggcaggc accggttttg gccacctggc gataccgcgt gtgggtcagg
    43201 aagtgattgt agacttcctc aacggcgatc cggaccagcc gatcattatg gggcgcacct
    43261 accaccagga aaaccgcccc cccggcagcc tgccgggaac aaagacgcag atgaccatcc
    43321 gctccaaaac gtacatgggc agcggattta atgagctgaa gtttgatgat gcgacaggga
    43381 gagaacaggt ctacatccac gcgcagaaga acatggatac cgaagtgctc aacgaccgta
    43441 ccaccaccgt aaaacacgat caccgcgaaa ccgtaaaaaa tgaccagacg gtcacgatcc
    43501 aggaaggtaa ccgccttctt acggtggaaa aaggccacaa gatcaccgga gtactgaaag
    43561 ggtctttatc tgaggatgtc tttcaggaca gaggcacgat tgccggttcg gtgcatgttg
    43621 acgctgtaaa caatggtggc gaaggcgacg gtatacaggc ttatacggcg attaaggaaa
    43681 ttttgctggc cgtggaggaa agcaaaattg cgctgacgcc ggatagcatt cagctacagg
    43741 tcggggaatc gacggtaatc aggctgtcga aggatggcat caccatcgtg ggcggttctg
    43801 ttttcatcaa ctgattcact cagattatca gggagtattt tatgcagttc acttttaatg
    43861 aaggccacat tcaactgccg tctcaatggc aggatcagtc aatgcaggtc ctggtgtcca
    43921 cagataacag tggcatcaat ctggtcatta cccgcgaagc cgttccacag ggaaccctga
    43981 caccagagct gtaccaggaa acgctggcgc tgtaccaggg caaacttgat gggtacaccg
    44041 aacatgcctg ccgggaaatc actctggcag aggccccggc ctggctgctg gattattcct
    44101 ggaacggtcc ggaagatgag gggaatcagg ggcggattag ccagatagcc gtattccagc
    44161 ggcgtggaga tacgctgctg accttcactt tcagcacctc cttatccctg aagaacagtc
    44221 aaaagacgat gctgctggag gtgataaaga gcttcacccc gttgccaccg gagaatgaca
    44281 tccaaaagga ccagccgcgc tgacataaac gagggataac acatgagtga aggaccaggc
    44341 gggccacagg gagcgaccgc aggcggtacg ctggcaatgc gaatgctgtc acagcaggcg
    44401 atggtcgcca gccagatgaa acgggcagcc aacgacaaag ccattgcaca gatgttggca
    44461 tcaaaaaagt ctggcccccc cgccgccagg ctgggcgatg aaattcagca caagagtttt
    44521 ttgggggcgc tggcaggggc cgtactgggg gcgatagtga ccatcgcaga aggttgcctg
    44581 attatggccg cctgtgccac cggcccttat gcgctggttc tggtacctgc gctgatgtat
    44641 gccagctata aggcgagtga ttatgtggag gagaaacaga accagcttga atcatggata
    44701 aacagctttt gtgacacgga cggcgccatc aataccggtt ctgaaaacgt aaaaattaac
    44761 ggcgagctgg ccgcacgtgc agcggtcacc cttccccctc ctcccccacc tggagcaata
    44821 cctgaagtcc cacaggggga accctcatgg ggtgatattg ccactgacct gcttgaatcg
    44881 gcagcggaaa aagcagtacc actggcgaag gcctggggga acgctgttat caccctgacg
    44941 gacagcaatg ccggttttat ggatcgcgtc agcgccggcg catcgcttct gtttcccgcc
    45001 ggtccggtat taatggagtt tgccaccatg gtgggcgggc gtggcgaaat caaaaaagat
    45061 gtggatttcc cggaagccgg tgaggacacg gcgctctgcg acaaggagaa caaaccaccg
    45121 aggatagccc agggcagcag caacgtcttt atcaacaatc agcctgccgc gcgcaagggc
    45181 gacaaactgg agtgcagtgc ggcgatcgtg ggaggttcgc cggacgtctt tattggcggt
    45241 gagcaggtca catatctgga tatccagccg gagttcccgc cgtggcagag aatgatcctg
    45301 gggggaataa cgatagccag ctatcttctg ccgccagcgg gactactggg aaaactgaag
    45361 aatctggcga gactcggcaa actgggaaac tgctggggaa aagcgggaag ctgctgggcg
    45421 caaagctcgg cgcgttgctg gggaaaacag gtaagtcgtt aaaaagtatt gccaataaag
    45481 tcatcagatg ggtaacagat cctgtcgatc cggtaaccgg cgcatactgc gacgaacgta
    45541 ccgacttcac cctgggccag accctccccc tctccttcac ccgtttccac agttctgtac
    45601 tgccgctgca tggcctgacc ggcgtgggct ggagcgactc ctggagcgaa tacgcctggg
    45661 tgcgtgaaca gggaaaccgg gtggatatca tcagccaggg agccacgctg agatttgcct
    45721 tcgacggtga cagtgatacg gcggttaacc cgtatcacgc ccagtacatt ctgcgccgcc
    45781 gcgatgatta tctggagctg ttcgacaggg atgcactgag cagccgcttc ttttatgacg
    45841 cctttccggg aatgcgtctg cgccacccgg tgactgacga taccagcgat gaccgcctgg
    45901 cacacagccc caatgaccgg atgtacatgc tgggcgggat gagcgacacc gccagcaacc
    45961 gcatcacgtt tgagcgcgac agccagtacc ggatcacggg tgtcagtcac accgacggga
    46021 tccggcttaa actgacgtac cacgccagcg gctacctgaa agccattcac cgcacggata
    46081 acgacataca gacgctggcg acctacgaac aggatgcgcg ggggcggctg acagaagcgg
    46141 atgcgcggct ggactaccac ctgttttatg agtacgacgc tgcggaccgg atcatccgct
    46201 ggtccgataa cgaccagacg tggagccgtt tcacctacga tgaacagggc cggtgcgtga
    46261 atgtcaccgg ggcggagggc tattacaacg ccacgctgga ctatggtgac ggctgcacca
    46321 ccgtgacgga cggcaagggc attcaccgtt attactatga tcctgacggc aatattctgc
    46381 gggaagaagc gccggacggc agcaccacca tgtatgaatg ggatgaattc catcacctgc
    46441 tggcccgcca ctcccctgcc gggcgggtgg agaaatttga atacaacgcc gcacacggtc
    46501 agttaagccg ttacacggcg gcagacggcg cggagtggca gtaccgctat gatgagcgcg
    46561 gcctgctcag caacatcacc gaccctgccg ggcagacgtg gacacagcag tgtgatgaac
    46621 gcggcctgcc ggtaagtctg gtgtcgccac agggcgaaga gacccggctg gcatacaccg
    46681 ctcaggggct gctgtcggga atattccgcc aggatgaacg gcgtctgggc atagagtacg
    46741 accaccacaa ccggccggaa acactcaccg acgtgatggg ccgtgaacac cacaccgaat
    46801 acagcggtca cgacctgcca gtgaagatgc gcggccccgg cggtcagtca gtgcggttac
    46861 agtggcagca gcaccataaa ctgagcggca ttgaacgtgc tggaaccggc gcggaaggat
    46921 ttcgctatga ccgccacggc aacctgctgg cctggacgga cggcaacggt gttgtctgga
    46981 cgatggaata cggtccgttc gattttccgg tggcgcgaac ggacggtgaa ggccaccgct
    47041 ggcagtaccg ctacgataaa gacacgctgc aactgacaga agtcatcaac ccgcagggtg
    47101 agtcatatct ttatgttctg gataactgcg gccgggtgac ggaagagcgt gactggggcg
    47161 gcgtggtctg gcgttaccgc tatgacgccg atggcctctg taccgccaag gttaacggcc
    47221 tggaggaaac catcctctac agccgggacg ccgcaggccg cctggcagaa atcatctcgc
    47281 cggaaggcaa aacgcagtat gcctatgaca aatccggcag gctgacgggt atcttcagcc
    47341 cggacggcac atcacagcgc accggctatg acgaacgcgg gcgggtgaat gtcaccactc
    47401 agggccgacg ggccattgaa tacaactacc ccgatgaaca caccgtcatc cgctgtatcc
    47461 tgccaccgga agatgaacgc gacagacacc ccggtgaatc cctgctgaaa accacgtacc
    47521 gctacaacgc cgccggagaa ctgacagagg tcattctgcc gggggatgag acgctgacgt
    47581 tcagccgtga tgaggcggga cgtgaagtgc tccggcacag taaccggggt tttgcctgtg
    47641 aacagggctg gaatgcagcc ggtcagcctg tcagtcagcg cgccggattt tttccggcag
    47701 aagccacatg ggacgggctg gttccctcac tggtacggga gtaccgttac gacagcgcgg
    47761 gtaacgtgtc aggcgtcacc agccgggaag attacggacg ggaaacacgg cgggagtatc
    47821 ggctggaccg gaacggccag gtcacggcgg tgacagcctc aggcaccggg ctgggctatg
    47881 gcgaaggcga tgagtcctat ggctatgaca gctgcggcta cctgaaggcg cagtctgcgg
    47941 gcaggcaccg gataagtgaa gagactgacc agtatgccgg aggccaccgg ctgaaacagg
    48001 ccggaaacac gcagtatgac tatgacgccg caggccggat ggtcagccgc accagacacc
    48061 gtgacggcta ccgaccggaa acagagcggt tccggtggga cagccgggac cagctgaccg
    48121 ggtattgcag cgcacagggt gagctgtggg aataccgcca cgacgccagc ggcagacgaa
    48181 cagagaaacg ctgcgaccgg aagaaaatcc gcttcacgta tctgtgggac ggcgacagta
    48241 ttgcggaaat ccgggaatac cgcgatgata aactgtacag cgtaaggcac ctggtgttta
    48301 acggctttga gctgataagc cagcagttca gccgggtacg gcaggcgcat ccgtcggtgg
    48361 ccccgcagtg ggtgacgcgg acgaatcatg cggtgagcga cctgacgggc cgcccgctga
    48421 tgctctttaa cagtgaagga aagacggtct ggcgaccggg gcagaccagc ctgtgggggc
    48481 tggcactcag cctgcccgca gacaccggct acccggaccc gcgcggggaa ctggacccgg
    48541 aagccgaccc cggcctgctg tatgcaggac agtggcagga tgcggaatcc gggctgtgct
    48601 ataatcggtt ccggtattac gagccggaaa ccggaatgta cctggtgagt gatccgctgg
    48661 ggttgcaggg cggggagcag acttaccggt atgtgccgaa tccttgtggg tgggttgatc
    48721 cgctgggatt ggctgcaagt tctaaaatca gcagtttgat ggactatatt ggcgatggtc
    48781 gtcgtgttag tgggcatacg ggtttcctgg atggggttcg tttatcacgt agtcaaataa
//
LOCUS       DAZPJR010000009        48814 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPJR010000009 DAZPJR010000000
VERSION     DAZPJR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.010"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            147..1490
                     /gene="argG"
                     /locus_tag="ACHVB7_000523"
     CDS             147..1490
                     /gene="argG"
                     /locus_tag="ACHVB7_000523"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR1912616.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1498..3124)
                     /locus_tag="ACHVB7_000524"
                     /pseudo
     CDS             complement(1498..3124)
                     /locus_tag="ACHVB7_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(3583..3669)
                     /locus_tag="ACHVB7_000525"
     tRNA            complement(3583..3669)
                     /locus_tag="ACHVB7_000525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3633..3635),aa:Leu,seq:gag)
     gene            complement(3684..4016)
                     /gene="secG"
                     /locus_tag="ACHVB7_000526"
     CDS             complement(3684..4016)
                     /gene="secG"
                     /locus_tag="ACHVB7_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR1912617.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4244..5581)
                     /gene="glmM"
                     /locus_tag="ACHVB7_000527"
     CDS             complement(4244..5581)
                     /gene="glmM"
                     /locus_tag="ACHVB7_000527"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR1912618.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5574..6422)
                     /gene="folP"
                     /locus_tag="ACHVB7_000528"
     CDS             complement(5574..6422)
                     /gene="folP"
                     /locus_tag="ACHVB7_000528"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR1912619.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6512..8446)
                     /gene="ftsH"
                     /locus_tag="ACHVB7_000529"
     CDS             complement(6512..8446)
                     /gene="ftsH"
                     /locus_tag="ACHVB7_000529"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR1912620.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8546..9175)
                     /gene="rlmE"
                     /locus_tag="ACHVB7_000530"
     CDS             complement(8546..9175)
                     /gene="rlmE"
                     /locus_tag="ACHVB7_000530"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR1912621.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9301..9594
                     /gene="yhbY"
                     /locus_tag="ACHVB7_000531"
     CDS             9301..9594
                     /gene="yhbY"
                     /locus_tag="ACHVB7_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR1912622.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9750..10226)
                     /gene="greA"
                     /locus_tag="ACHVB7_000532"
     CDS             complement(9750..10226)
                     /gene="greA"
                     /locus_tag="ACHVB7_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR1912623.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10474..11907
                     /gene="dacB"
                     /locus_tag="ACHVB7_000533"
     CDS             10474..11907
                     /gene="dacB"
                     /locus_tag="ACHVB7_000533"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1912624.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11962..12069
                     /locus_tag="ACHVB7_000534"
                     /pseudo
     CDS             11962..12069
                     /locus_tag="ACHVB7_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12093..13265)
                     /gene="cgtA"
                     /locus_tag="ACHVB7_000535"
     CDS             complement(12093..13265)
                     /gene="cgtA"
                     /locus_tag="ACHVB7_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR1912625.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13281..14246)
                     /locus_tag="ACHVB7_000536"
     CDS             complement(13281..14246)
                     /locus_tag="ACHVB7_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1912626.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14373..14630)
                     /gene="rpmA"
                     /locus_tag="ACHVB7_000537"
     CDS             complement(14373..14630)
                     /gene="rpmA"
                     /locus_tag="ACHVB7_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR1912627.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14651..14962)
                     /gene="rplU"
                     /locus_tag="ACHVB7_000538"
     CDS             complement(14651..14962)
                     /gene="rplU"
                     /locus_tag="ACHVB7_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR1912628.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15221..16192
                     /gene="ispB"
                     /locus_tag="ACHVB7_000539"
     CDS             15221..16192
                     /gene="ispB"
                     /locus_tag="ACHVB7_000539"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR1912629.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16421..16699
                     /gene="sfsB"
                     /locus_tag="ACHVB7_000540"
     CDS             16421..16699
                     /gene="sfsB"
                     /locus_tag="ACHVB7_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR1912630.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16747..18006)
                     /gene="murA"
                     /locus_tag="ACHVB7_000541"
     CDS             complement(16747..18006)
                     /gene="murA"
                     /locus_tag="ACHVB7_000541"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR1912631.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18061..18315)
                     /gene="ibaG"
                     /locus_tag="ACHVB7_000542"
     CDS             complement(18061..18315)
                     /gene="ibaG"
                     /locus_tag="ACHVB7_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR1912632.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18475..18768)
                     /gene="mlaB"
                     /locus_tag="ACHVB7_000543"
     CDS             complement(18475..18768)
                     /gene="mlaB"
                     /locus_tag="ACHVB7_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR1912633.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18768..19403)
                     /gene="mlaC"
                     /locus_tag="ACHVB7_000544"
     CDS             complement(18768..19403)
                     /gene="mlaC"
                     /locus_tag="ACHVB7_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR1912634.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19422..19973)
                     /gene="mlaD"
                     /locus_tag="ACHVB7_000545"
     CDS             complement(19422..19973)
                     /gene="mlaD"
                     /locus_tag="ACHVB7_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR1912635.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19978..20760)
                     /gene="mlaE"
                     /locus_tag="ACHVB7_000546"
     CDS             complement(19978..20760)
                     /gene="mlaE"
                     /locus_tag="ACHVB7_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR1912636.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLANCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20768..21577)
                     /gene="mlaF"
                     /locus_tag="ACHVB7_000547"
     CDS             complement(20768..21577)
                     /gene="mlaF"
                     /locus_tag="ACHVB7_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR1912637.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21787..22764
                     /locus_tag="ACHVB7_000548"
     CDS             21787..22764
                     /locus_tag="ACHVB7_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR1912638.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22778..23764
                     /gene="kdsD"
                     /locus_tag="ACHVB7_000549"
     CDS             22778..23764
                     /gene="kdsD"
                     /locus_tag="ACHVB7_000549"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR1912639.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23785..24351
                     /gene="kdsC"
                     /locus_tag="ACHVB7_000550"
     CDS             23785..24351
                     /gene="kdsC"
                     /locus_tag="ACHVB7_000550"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR1912640.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24348..24923
                     /gene="lptC"
                     /locus_tag="ACHVB7_000551"
     CDS             24348..24923
                     /gene="lptC"
                     /locus_tag="ACHVB7_000551"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR1912641.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24892..25449
                     /gene="lptA"
                     /locus_tag="ACHVB7_000552"
     CDS             24892..25449
                     /gene="lptA"
                     /locus_tag="ACHVB7_000552"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR1912642.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25456..26181
                     /gene="lptB"
                     /locus_tag="ACHVB7_000553"
     CDS             25456..26181
                     /gene="lptB"
                     /locus_tag="ACHVB7_000553"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR1912643.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26229..27662
                     /gene="rpoN"
                     /locus_tag="ACHVB7_000554"
     CDS             26229..27662
                     /gene="rpoN"
                     /locus_tag="ACHVB7_000554"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR1912644.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27685..27972
                     /gene="hpf"
                     /locus_tag="ACHVB7_000555"
     CDS             27685..27972
                     /gene="hpf"
                     /locus_tag="ACHVB7_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR1912645.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28090..28581
                     /gene="ptsN"
                     /locus_tag="ACHVB7_000556"
     CDS             28090..28581
                     /gene="ptsN"
                     /locus_tag="ACHVB7_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR1912646.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28627..29481
                     /gene="rapZ"
                     /locus_tag="ACHVB7_000557"
     CDS             28627..29481
                     /gene="rapZ"
                     /locus_tag="ACHVB7_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR1912647.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29478..29750
                     /gene="npr"
                     /locus_tag="ACHVB7_000558"
     CDS             29478..29750
                     /gene="npr"
                     /locus_tag="ACHVB7_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR1912648.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29964..30596
                     /gene="yrbL"
                     /locus_tag="ACHVB7_000559"
     CDS             29964..30596
                     /gene="yrbL"
                     /locus_tag="ACHVB7_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR1912649.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30593..31321)
                     /gene="mtgA"
                     /locus_tag="ACHVB7_000560"
     CDS             complement(30593..31321)
                     /gene="mtgA"
                     /locus_tag="ACHVB7_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR1912650.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31318..31971)
                     /gene="elbB"
                     /locus_tag="ACHVB7_000561"
     CDS             complement(31318..31971)
                     /gene="elbB"
                     /locus_tag="ACHVB7_000561"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR1912651.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32201..34537)
                     /gene="arcB"
                     /locus_tag="ACHVB7_000562"
     CDS             complement(32201..34537)
                     /gene="arcB"
                     /locus_tag="ACHVB7_000562"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR1912652.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34633..35562)
                     /locus_tag="ACHVB7_000563"
     CDS             complement(34633..35562)
                     /locus_tag="ACHVB7_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR1912653.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTFTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36237..40697
                     /gene="gltB"
                     /locus_tag="ACHVB7_000564"
     CDS             36237..40697
                     /gene="gltB"
                     /locus_tag="ACHVB7_000564"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR1912654.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40710..42128
                     /gene="gltD"
                     /locus_tag="ACHVB7_000565"
     CDS             40710..42128
                     /gene="gltD"
                     /locus_tag="ACHVB7_000565"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR1912655.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42312..42716
                     /locus_tag="ACHVB7_000566"
                     /pseudo
     CDS             42312..42716
                     /locus_tag="ACHVB7_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            42808..43113
                     /locus_tag="ACHVB7_000567"
     CDS             42808..43113
                     /locus_tag="ACHVB7_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1912656.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43189..43653)
                     /gene="nanQ"
                     /locus_tag="ACHVB7_000568"
     CDS             complement(43189..43653)
                     /gene="nanQ"
                     /locus_tag="ACHVB7_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR1912657.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43650..44525)
                     /gene="nanK"
                     /locus_tag="ACHVB7_000569"
     CDS             complement(43650..44525)
                     /gene="nanK"
                     /locus_tag="ACHVB7_000569"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR1912658.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44522..45211)
                     /locus_tag="ACHVB7_000570"
     CDS             complement(44522..45211)
                     /locus_tag="ACHVB7_000570"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR1912659.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45259..46749)
                     /gene="nanT"
                     /locus_tag="ACHVB7_000571"
     CDS             complement(45259..46749)
                     /gene="nanT"
                     /locus_tag="ACHVB7_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR1912660.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46859..47752)
                     /gene="nanA"
                     /locus_tag="ACHVB7_000572"
     CDS             complement(46859..47752)
                     /gene="nanA"
                     /locus_tag="ACHVB7_000572"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR1912661.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47874..48665)
                     /gene="nanR"
                     /locus_tag="ACHVB7_000573"
     CDS             complement(47874..48665)
                     /gene="nanR"
                     /locus_tag="ACHVB7_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR1912662.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        12165 a        13126 c        12059 g        11464 t
ORIGIN      
        1 aagcgcatta ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta
       61 aatgaaaact catttatttt gcataaaaat tcagtgagag cggaaatcca ggctcatcat
      121 cagttaatta agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc
      181 aacgtattgg tatcgctttt tccggcggtc tggacaccag tgccgcactg ctgtggatgc
      241 gacaaaaggg agcggttcct tatgcatata ctgcaaacct gggccagcca gacgaagagg
      301 attatgatgc gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg
      361 actgccgcaa acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata
      421 acaccaccgg cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta
      481 ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct
      541 ataaaggaaa cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc
      601 agatttacaa accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga
      661 tgtctgaatt tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact
      721 ccacagactc caacatgctt ggtgcaacgc acgaagcgaa ggatctggaa tacctcaact
      781 ccagcgtcaa aatcgtcaac ccaattatgg gcgtgaaatt ctgggatgag agcgtgaaga
      841 tcccggcaga agaagtcaca gtacgctttg agcaaggtca tccggtagcg ctgaacggta
      901 aaacctttag cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg
      961 gcctgggcat gagcgaccag attgaaaacc gtatcatcga agcgaaaagc cgtggtattt
     1021 acgaagctcc ggggatggca ctgctacata tcgcttatga acgtctgttg accggtattc
     1081 acaacgaaga caccattgag cagtatcatg cacatggtcg tcagttgggc cgtctgctgt
     1141 accaggggcg ttggtttgat tcccaggcgc tgatgctgcg tgactctctg caacgctggg
     1201 ttgccagcca gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa
     1261 tcctgaatac cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag
     1321 gcgactcggt gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aacctggata
     1381 tcactgatac ccgagagaaa ctttttggtt atgccaaaac tggcctgctc tcctcctctg
     1441 ccacttcagg cgtgccgcag gtggagaatc tggaaaacaa aggccagtaa ttcgacatca
     1501 accctgccct tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat
     1561 cacataatcg aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc
     1621 aatcacttcc tccagcgttt tcggttgttc cggctttgtt acccccatcc acgcattaat
     1681 aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc gttgtacgat ccacgccatc
     1741 cccaagaacg gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc
     1801 cctgcctcca tgaaaataga cgttcttctt cgtagggtca cgttccaggc cgtggtcggc
     1861 aaaatacatg accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact
     1921 atctgtgtaa tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac
     1981 ggcgctggat tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac
     2041 aatcagtttc ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa
     2101 ttcatcaaat cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc
     2161 tgtaccattc tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg
     2221 attagccata ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc
     2281 agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgattgatt
     2341 aaacagtttg atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc
     2401 atacaaagac atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat
     2461 acctgtatcc ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct
     2521 ttgatgctct ttcgctgcta aagcaaaata attgagattg aaaaacggcg tataggtagc
     2581 aaatcgcgac gctagtatat atggactgaa tgctttttta ttttttgcat ctttataagc
     2641 aaattgacaa gcagaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac
     2701 tttttttgtc ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa
     2761 aaaagcaaaa gcacatagat aagggatata catccccagc attttgacaa cttcatccgg
     2821 atcactctgc agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca
     2881 tgaatagagg ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag
     2941 caggcgaagc ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag
     3001 caatcgctga accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat
     3061 aacacaaaga tacaacaacc aatgagattt aaaatcttct agcaaatttg ttgaatactg
     3121 tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat
     3181 atcagacatg tttttcaact ctgatccata tcagaccgaa acactgttag ttttttcttt
     3241 ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg cgtctctaaa
     3301 cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg tttatcgaga
     3361 agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg ccaattatgt
     3421 cagcagaagg gaaaagttta taactaaggc gtaccggcac catcgtttca aggtaccagc
     3481 tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca gaccaggacc
     3541 aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg aggacgggac
     3601 ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta ccaattccac
     3661 cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt agccggagca
     3721 gctggctgag tttgttcggt tttcgccggt gcactcagat tttcccattc gctaccttta
     3781 ttggttttgt tgctattgat gttacccagc accagactga tgataaagaa taacgttgcc
     3841 agcagcgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg
     3901 gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg cagcatgatc
     3961 agaccaacaa ggccaattgc cacaataagg aaaactacca aaagagcttc gtacataatc
     4021 aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc gggagttttt
     4081 aatctttccc actgaagtgg gtgtgaatac taaccaaagc gaatgacctt cgcaagggca
     4141 attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt tcgctgagaa
     4201 aaaaggcgac aatcgccgcc tttttagcca gttatttacc gttttaaacg gcttttactg
     4261 catcggcgat acggtgtgca aattcagtta cctgcgcttc gtcttcacct tctaccatca
     4321 cgcgaattaa cggttcggtg ccggatttac gcaacagcac acgtccacgg ctacccagcg
     4381 ccgcttcaac ttcggcggtc acagctttaa ctgactcatg ctcaagcgga tcgccgctac
     4441 ctgcggtgta acgcacgtta accagaatct gcgggaacat tttcatgccg ctgcaaaggt
     4501 cgtgcaggct catatggtta cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc
     4561 cgtcaccggt agtggtttta tccagcagga tcacatgacc ggaattctct gcaccgatac
     4621 gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc gcgcgcgcaa
     4681 atggaattcc taactgtttc aacgccagct caagccccat gttactcatc aatgtaccca
     4741 cagcaccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata tacatgatct
     4801 gatcaccatc gactttattg ccttcatggt caaccataat cacgcgatcg ccgtcgccgt
     4861 cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc gcgcgaacgt
     4921 cggtagcccc cacttcggca ttgatgttta caccgttcgg ctcacaaccg atagcgataa
     4981 cgttcgcccc cagttcacgc agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcgc
     5041 aatccaccac aatcttcagt tcgctgaggc taagttcgtt cgggaacgtg gctttgcaaa
     5101 actcgatata gcgacccgca gcatcaacga tacggctggc tttacccagt tctgccgaat
     5161 caacacagct gatctctttt tccatttccg cttcgatggc ctcttctacc gcatccggta
     5221 gtttggtgcc gtcgatagag aaaaatttaa tgccattatc gtagaacggg ttatgcgatg
     5281 cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc acggccggtg
     5341 ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg
     5401 ctgactccag catatagcca gaaatacgcg tgtctttacc aataatgatc ttacgggagc
     5461 cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa tcaggtgtga
     5521 tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta cgattactca
     5581 tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc ttctacggtt
     5641 tctttgacgt catgaacacg aataatgtgc gcgccttgca ttgcggcaat gaccgcacag
     5701 gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc aatcatcgat
     5761 tttcgtgaca tacctaccaa cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc
     5821 agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc gagcaacaat
     5881 ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc
     5941 acttctgcaa agacatcgtc atacttcgga gcttcctgca tggtttttgg atttccctgc
     6001 atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc gccaggttcg
     6061 gaaagggagc ggatatcgtt aataatgtga gcgccaactt tcgctgactc acggatgact
     6121 tctggtttgg atgtatctac cgagatccag acttcgaagc gttgagcaat tgcctcaacc
     6181 acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg
     6241 gactcgccac caacgtcaat gatcgtcgcg ccagcgttga tcatcagatt cgcatgtttc
     6301 accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg
     6361 aggatcccca ttacgtgagg atggctaagg tccagtgaag tgccctgggc aaagagtttc
     6421 atggtgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc ccggggtttt
     6481 cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc tctgacatgg
     6541 tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt ggactaccat
     6601 tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc
     6661 gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc atgagagcat
     6721 ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca cgattatagt
     6781 tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca tcggacatat
     6841 gtttcgcttt cgctacgcta cggccgagga acacttcacc ttcttcttcc gcgtacagca
     6901 gtggacccaa tttctcagag aagccccact gagtcaccat gttacgcgcc aggttggtcg
     6961 caactttaat atcgttggac gcaccggtag aaacatgttc cggcccgtag atgatctctt
     7021 ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga cggctggcgc
     7081 tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg cgtgggataa
     7141 tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatc gcatgacccg
     7201 cttcgtggta agccgtcgat tctttctgcg cttccgtcat caccatggag cgacgttccg
     7261 cacccatcat gattttatct ttcgctttct cgaactcaac catcgacaca acgcgtttgt
     7321 tgccacgagc agcgaacagt gccgcttcgt ttaccaggtt cgccaggtca gcaccggaga
     7381 aaccaggagt accacgggca atgattgccg cgtcgatatc gggtgccaat ggtacgcgac
     7441 gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc tggcaagcca accacaacct
     7501 gacggtcgaa acggccagga cgcagcaggg ccgggtcaag aacgtccgga cggttagtcg
     7561 cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc agcatctggt
     7621 tcagagtctg ttcacgttca tcgtgaccac cgcccagacc agcgccacgc tggcggccta
     7681 cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca
     7741 tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca gaaccggaga
     7801 tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcagc gttttaccgg
     7861 tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt ttctggaagc
     7921 ggctcggctc gcgcagatat tcaaccagtt cagcaacttc ttcttttgct tcgtcgcagc
     7981 ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc gctttgctct
     8041 taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc
     8101 acacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc agcaggcttg
     8161 gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta tccagtaatt
     8221 tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg gtaacgttga
     8281 tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct tgtaggaagg
     8341 tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg aatactgaca
     8401 tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca ctcaagggat
     8461 taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc cagcatcttt
     8521 caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca atatacactt
     8581 cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta aacagggagc
     8641 gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact acaaaactgc
     8701 cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga tacatggcac
     8761 gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac atgacaacct
     8821 ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca tcacgaaaat
     8881 cgccctgaag aaagtctaca ccaacgatag gatccatagg taaaagatcg caagcgatga
     8941 tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc ggagcagcac
     9001 caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt atttcatcaa
     9061 gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca tatttatcgc
     9121 taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct gtcatttaac
     9181 tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg
     9241 atatatggga gatggcggta gaatgaccca ttttcaatcc caacgtaagc aaaaaatacg
     9301 atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca
     9361 gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat tgaacaagcg
     9421 ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga aacaaaaacc
     9481 ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa
     9541 acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg ctaagattat
     9601 cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa
     9661 atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt tctttacaat
     9721 acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat tacttcaaat
     9781 tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt gccgatcagg
     9841 ccacgagcaa taggcgagtt tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg
     9901 ccaacgatgc gataagtctg ttcttcgtca gaatccagat tcagcaccgt tacggtagca
     9961 ccaaaaataa cgcgcccatt gttaggcatt ttggtgacat caatcacctg cgcgtttgac
    10021 agcttggctt cgatgtcttt gatacggcct tcgcagaagc cctgctgttc acgagctgcg
    10081 tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttcagcgat agcagcaatg
    10141 atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg
    10201 cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct gatggggcaa
    10261 gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg gaccagaagc
    10321 aaaaaaatac cgacccaggt acaagtccca ggtcagctac aattcacatt ttgatagtca
    10381 ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc gccgtagtat
    10441 gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt tatcatcgga
    10501 ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg ttgatgagta cattactcaa
    10561 ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc ggcccccgct
    10621 attgattacc acagtcagca gatggcgctg cctgccagta cccagaaagt gattactgcg
    10681 ctggcggcgt tgattcaact cggccccgat tttcgtttta ccacgacgct tgaaaccaaa
    10741 ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg tgccgatccg
    10801 acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac
    10861 caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga taaagccccc
    10921 ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc cgccatagtt
    10981 gaccgcaact gtttctccgt ctcgctctac agtgcccaaa aacccggtga tatggccttt
    11041 atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac cctcccccgt
    11101 ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac caggcgacct gaaccgcttt
    11161 acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt tgccgtgcag
    11221 gatggagcca gttatgccgg tgcaattctg aaagatgagt taaaacaggc gggtatcacc
    11281 tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt agttgccagt
    11341 aaacagtcgg ccccgctgca cgatctgctt aagattatgc tgaaaaagtc ggataacatg
    11401 atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtacc tggtacatgg
    11461 cgggccgggt cggacgctgt gcgtcagatc ctgcgccagc aagccggtgt cgatattgga
    11521 aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc ccccgccacc
    11581 atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt tatctccatg
    11641 ctgccgctgg cgggctatga cggctctttg cagtaccgtg caggtctgca tcaggcgggc
    11701 gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa cctggcggga
    11761 ttcattacca cagcgagcgg gcaacgaatg gcgtttgtgc aatatctttc tggctatgca
    11821 gtagaacctg cggatcagcg taatcgccgt attccgttag tgcgttttga aagccgtttg
    11881 tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg gccggggctt
    11941 cagattattg acaaagtgcg ctttgtttat gccggatgcg gcgtaaacgc cttatccggc
    12001 ctacaaaatc gtgcaaattc aacatattgc aattctcttg taggcctgat aagcgtagcg
    12061 catcaggctg atttggcgtt tatcatcagt gattaacgct tgtaaatgaa ctcaacgcct
    12121 tcttcgtcgt cttcgtccca gtcgtcatcc cagtcttcat catcctcttc agcaatctct
    12181 tcaagctgct ggcgatgata atcgtcccac atgaattcga ctttctctgg ctgtttcgct
    12241 tcttcagcct gcacgaccgg gttttcaagg ataaaggtca tcacatccca gcagagatct
    12301 ttcacgccca gtccgctcgc cgcagagatc agataatatt tatcttccca gcccagcgct
    12361 tcagcaatcg ctttcgcttt ctcttcagct tctgccttat ccagcagatc gatcttattg
    12421 aacactaacc aacgcggttt cgccgccaga tcctggctgt atttttccag ctcgctgata
    12481 ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg gatcgatatc gatgaggtgc
    12541 aacaggacgc ggcaacgttc caggtgcttc aggaagcgga tacccagacc tgcgccttcc
    12601 gcagcgcctt caatcagtcc tggaatatcg gcaacaacga agctcttttc gttgtccatt
    12661 cgtaccacac ccagacttgg caccagagtg gtaaacggat aatccgccac tttcggttta
    12721 gccgccgata ccgcgcgaat aaaggtcgat ttacccgcgt ttggcatccc caacataccg
    12781 acgtcagcca gcagcatcag ctccagcagc agctcacgct tatcacccgg cgtaccgttg
    12841 gttttctgcc gtggtgtacg gttaacggac gatttgaaac gggtattgcc cagaccgtgc
    12901 cagccgccct tagcaaccag cagacgctga ccgtgtttag tcatatcgcc catggtttca
    12961 ccagtaccct ggtcgattac acgcgtacct accggcactt taatcgtcac gtctttaccg
    13021 cgcttaccgg tacaatcgcg gcttgcgccg ttctgaccgc gctctgcacg gaaagatttt
    13081 tcaaaacgat aatcgataag cgtgttcagg ttttcgtcgg cttccatcca tacgtcacca
    13141 ccatcaccac cgtcgccgcc atccgggccg cctttcggaa tatacttttc gcggcggaag
    13201 ctcacgcaac cattaccgcc atcacctgca acgaccagaa tcgatgcttc atcaacaaac
    13261 ttcattttat tctccataaa tcattcgcct gcgcggggtt gcgataccac cgttgtatgc
    13321 ttacgtaatc cgccccaaat acgatgacca atggcggaat acatcgcgcc cgcaaccacg
    13381 acaaacgcac cgagataacc taaaaggttt aacatcggtc tggcgaagaa atcgggccag
    13441 gccagtgata aaagatctga aaaaaacagc gtaaacagtg gggtgagcgt gatgatcgcg
    13501 ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca gggcgccata tcctaccaat
    13561 gtattcagtc cgcaaaaaat taaacatgcg agctgccagt ggctaagctg cgctatcact
    13621 ccaggctttg ccagagggaa gagcgcaatt gtacataaag tgtacagtaa aaacaggatc
    13681 tgcggtgagg ccagccgacg caataaaacc ttttgcgcca cgccataact cacccaaacc
    13741 gtcgccgcac cgaccccaaa gataactccc caggtgtaat cggtgagctt tgtaaatatc
    13801 tcgaccagac tggtgttaaa aaacatcacc aggccgctca ggagcatcaa tgcccctaca
    13861 acctgagtgc tgcgcatttt ctctttcagg ataaatacgc tggcaaccat catgccaact
    13921 ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca ggtattgcaa ggatgagctg
    13981 aacaggatga agttcccaaa cagcccggcg gtcgccactg ccaacaaaat caaccagcgt
    14041 ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca ccgcaagaat ggcaccaagg
    14101 ccaatactcg ccatcaagaa acggtaaaac acgattgtcg gaggttccat cacctccagc
    14161 acctgcttca ttgcgattgg caacgccccc cagcaaattg ctgtggtgag cgccaaaaga
    14221 atgccaatgc ctgcctgctg cttcataccg tattccctgc aaagcgcatt tgcggattaa
    14281 cgtcgcacca tgcgcatttt taccggttat cgaatgtaaa aagccccgca acgtgttgcg
    14341 gggctttcat ccgttaccgg gacgcgaaaa acttattcag cttcgatgct gataaattta
    14401 cggtttttcg ggcctttaac ttcgaatttc actttaccgt ctgctttagc aaacagagtg
    14461 tggtcacgac cgcaacctac gttagcgcca gcgtggaatt tggtaccacg ttgacgaacg
    14521 atgatgctac ccgccagaac ggattcgcca ccgaaacgct taacgcccag gcgtttagct
    14581 tctgaatcgc gaccgttacg tgtggagccg ccagcctttt tatgtgccat ttgaaatctc
    14641 tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc agtgaaccac tgacgatgac
    14701 cctgctgctt acgatagtgt ttacgacgac gaaacttaac gattttaact ttctcgccac
    14761 gaccgtgagc aacaacttca gctttgatta cgccgccatc aacgaaagga acgccgattt
    14821 tgacttcttc accgtttgcg atcatcagca cttcagcgaa ctcaacagtt tcgccagttg
    14881 cgatgtccag cttttccagg cgaacggtct gaccttcgct tactcggtgt tgtttaccac
    14941 cactttggaa aaccgcgtac atataaaact ccgcttccgc gcacaccttt tcaatgattc
    15001 agagtgcgct ataaatattc acaatagggc gcgaatatta cgcaaaacgc acgcctttga
    15061 caagtgctac agtcaataca cgaagaaaaa aaacacaact tgtacggtaa cgtttatctg
    15121 tgccattttt tcagtacaat caccctatat tcctaaccat aaaccctaag ttgcctttgt
    15181 tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg atgaatttag aaaaaatcaa
    15241 tgagttaacc gcgcaagata tggcgggtgt taatgcggca atccttgagc agcttaattc
    15301 cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc agcggcggcg gtaaacgtat
    15361 tcgtccgatg attgctgtac tggctgcacg agctgttggc tatgagggaa atgcgcatgt
    15421 caccattgct gccctgatcg agtttatcca cacggcgact ctgctacacg acgacgttgt
    15481 ggatgaatca gatatgcgca ggggtaaagc taccgccaac gccgcatttg gcaatgccgc
    15541 cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc cagatgatga ccagcctcgg
    15601 ttcactcaaa gtgctggaag tcatgtcaga agctgtaaac gtcatcgcag aaggtgaagt
    15661 tctgcaactg atgaacgtta acgatccgga catcactgaa gaaaactaca tgcgcgttat
    15721 ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag tgttccggga ttctggctgg
    15781 ctgtacgccg gaggaggaga aaggcctgca ggattatggg cgctatctcg gcactgcttt
    15841 ccagttgatc gacgatttac tcgattacaa tgccgatggc gaacagttag gtaaaaatgt
    15901 cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg ctgcatgcga tgcatcatgg
    15961 cacaccagaa caggcacaga tgatccgtac cgccatcgaa cagggtaacg gtcgccatct
    16021 tctggaaccg gttctggaag caatgaacgc ttgtggatct cttgaatgga cgcgtcagcg
    16081 tgccgaggaa gaagcagaca aagccatcgc agcattacag gtgctcccgg acaccccttg
    16141 gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa cgcgatcgtt aatcccctcc
    16201 cttcatcccg cgcagtgcgc gggatgattc cagtaaattc cataaaaaca ctaattcagc
    16261 gctataacac ctgaaaaaaa agggcgatga atctttatat attctgaata ttcacactct
    16321 ttacaggaac tttttagagc aataggccat caggagtata gtgatgcccg acagaagaag
    16381 tgttctgaat gaaagcgact aacttaagga gtgaggaaag atggaaagta atttcattga
    16441 ctggcatccc gctgacatca ttgcgggttt gcgcaagaag ggaacgtcaa tggcggcgga
    16501 gtctcgcaga aatggtttga gttcctcaac gctggcaaac gcattatcgc gcccatggcc
    16561 gaaaggagag atgattattg cgaaagccct gggaactgac ccctgggtta tctggccatc
    16621 acgctaccat gatccgcaga cccatgagtt tatcgacaga acgcagttga tgcgtagcta
    16681 cactaaaccg aaaaaatgag tggtctggcg gtagccccgc gaacggggct gccagctctc
    16741 agacgattac tcgcctttca cacgctcaat atttgcacct aaagcgcgca gtttgtcttc
    16801 aatgcgttcg tagccacgat cgatgtgata aatacgatca accaccgtcg tcccttccgc
    16861 aatacagcca gccagcacca ggcttgctga tgcacgcaga tcggttgcca taacctgtgc
    16921 gccagaaagt ttttcaacac cgtgacaaat aacggtattg ctttcgattt cggcatgcgc
    16981 gcccatacgg ctaagctccg gcacatgcat aaagcggttt tcaaagaccg tttcggtgat
    17041 gaatccggtc ccttctgcca ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg
    17101 gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc catgcatatc
    17161 caggctaatc cagtcttcgc cgacttcgat gtccgctcca gcgtcacgca gtttcgccag
    17221 cacggcgtcg agagtatctg gctgcgcgtt acggcagata attttgccgc gagaaatcgc
    17281 cgccgccacc aggaaagtac cggtttcgat acgatccggt agcacgcgat agacaccgcc
    17341 gcctaaacgt tccacacctt cgatgacgat acgatcggtg ccctgaccgc taattttcgc
    17401 acccagcgta atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg ctgcattttc
    17461 aataatcgtg gtaccttccg ccagggttgc agcacacatg atggtcaccg ttgcgccaac
    17521 gctgacttta tccatcacga tatgtgcgcc tttcagacga ccatcgacgg aagctttaac
    17581 gtaaccttct tccagtttga tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc
    17641 aaccggacgc gcaccgatcg tacaaccgcc aggcagtgaa acttgcccct gaccaaagcg
    17701 cgctaccagc ggccccagtg cccagataga agcacgcatg gttttaacca gatcgtaagg
    17761 tgcgcagaat acattaacgt cgcgggcatc aatatgcaca gaaccattac gttctacttt
    17821 cgcacccagc tggcttagca gcttcattga tgtatcgacg tctttcagtt ttgggacgtt
    17881 ctggatctct accggttctt ccgccagtag agcggcaaaa aggataggca gagcagcatt
    17941 tttagcgccg gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt
    18001 atccatttag tttgttctca gttaacaatt catatccgct accggcgaat cgcccatagc
    18061 tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg ctttgatcga
    18121 cacagcatga atgcggttat ccgcaatata ttccatcagc gggccataga ccgtctgctg
    18181 ttttttaacc cgactcatgc cgtcaaacaa ctcacccacg gcaataacct gaaagtggct
    18241 gccatcgccg gaaacgtgga cttcctggag ggagagagcg ttcatcaaca cgctctgaat
    18301 ttcattattt tccatgggat cttcaatcat cagttaataa accagcgaaa catcttagag
    18361 caaagttgcg ctggcataaa taagcaaaaa gcctcgctga taaatcagac aaggctcgac
    18421 ttgcaggcag gtttgccgga caggcggtta acgccatatc cggcctgaaa aaatttaacg
    18481 aggcagaaca tcagcaggca aattatacaa tttcgcgagg gtatacactt tgtcgtttac
    18541 cccctgaagc gtcacgttgt tgccctgctt tttcgccaga tcgataagat ggagcagcag
    18601 tgccagtcca cccgtatcca cgcgggacac acggctaaga tcgatgcagg taatcccctt
    18661 caccgcttcc tcacgcattt cccaaagcgg tagcagaacg tcctgatcca gctctccgga
    18721 taacgccagc gtgtcacccg tctgcatcca gctcagtgac tcgctcatta ttttttctct
    18781 tccagagtga ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct
    18841 ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg tgatcatact gacgccttca
    18901 gcaatcatgt cgtaagcctg ccagtttccc gtctgggagt ttttacgcca ctggaagtcc
    18961 agacgcaccg gcggacggcc attcgggtca ataatggtaa cgcgaatagg cacaatggtt
    19021 ttatcgccca gcggctgttc tggcgcaatc tgataggttt gaccgtgata catcgccagc
    19081 gcctgaccgt aagcctgctt caggtactca cggaaagcgg caaagtaggc ttcacgttga
    19141 gcaggggtcg cactcttgta atactggccc agcaccagcg caccggcgta tttcacctgt
    19201 acgtatggca gcagttcctg atcaacaatg gtacgcagat aatccgggtt ggcccgaatt
    19261 tgcggttgct cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc catcagctta
    19321 taaggattgg tctggtctgc cgcggttgcc gcactcagag gtgcaatcac cagcaaagcg
    19381 accatcatta aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt tgtacccaca
    19441 ggttcagtgg tttcattatt acctggcgca gcagctggcg catcgccact attcttattg
    19501 tcatcgcctt tactaccgta aaggaactga ccaatgagat cttccagcac catcgcagac
    19561 ttagtgtcct gaattgtatc gccatccttc aggatagcag tccccagttc cgggtcttca
    19621 aaaccgacgt ttaatgccag atattgttcc cccagcaggc cggaagtacg aatgctcagc
    19681 gaactggtat ctggaatgtg gttataacgt tgttcaattt ccagcgttac gcgcggcaga
    19741 taggttttcg ggtccagcgt aatatccgcc acccgaccca caacaacgcc accaatactg
    19801 accggagagc gcgctttcag gccgccaatg ttatcgaacg tcgcataaag cgtgtaggtc
    19861 ggttcagtac gtatggacgt cacgttcgcc gccttcaggc aaacaaacag cgccgccagc
    19921 agtgctgcta ataaaaagat acccacccaa atttcatttt ttttcgtttg catgaactca
    19981 attcccaaac atcaatgcgg tcagcacaaa atccagcccc agaacagcca gagacgagtg
    20041 gacaacggtg cgagtggttg cccggctaat cccggcagac gtcgggatgg tgtcgtagcc
    20101 gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt
    20161 ggccagatcc atacgccagt cgacggcatt ttgcattgcc gaccagaaga acccgctatc
    20221 aatgcctttc caactgacgc cgaccagaga tccgccccag atccccacgg cgacgaaaat
    20281 aaccgtcaac agtggtaatg aaataacccc agcccagaaa cggggagaaa taacccgacg
    20341 cagcggatcc accgccatca tctccatact ggagagttgc tctgtagcgc gcatcaggcc
    20401 gatttctgcg gttagcgccg aaccagcacg cccggcaaac aacaacgcgg caaccaccgg
    20461 ccccagttca cgcagtagcg ataacgccac cagcataccc agactggttt ccgcactata
    20521 agtggtcaga accagataac cttgcagccc caacaccatt ccgatgaaca cgccagaaac
    20581 cacaataatc agcatcgaca ggacgccgac attatagagc tggcgcacca gcagcggcgc
    20641 atgtttgcga aattccggtt tgccgaccag cgcattgaat aacattaacc cggcccgccc
    20701 gaacgttctc agggttttaa tccctttatg tccgagcgac gccagcgcat ttaacagcat
    20761 gagtggctta actccctggt aaaagatcag cgtgataatc gccggcagga tagcggaacg
    20821 gaacaggccc gtcagctatc ccgtccagaa actgacgtac gcgcggatca ggattcgcct
    20881 gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc cgccaggatc caggcgtgat
    20941 ccgcaatact taacacttcc ggcacatcgt gagaaaccac cacacaagtc acgcccagcg
    21001 cgctgttcag ctcagaaatc agcttcacca gtacgcccat ggtgatggga tcttgcccaa
    21061 caaaaggttc atcaaacatg atgagatccg gctccagcgc aatcgcacgc gccagcgctg
    21121 cacgccgcgc catcccaccg gaaagttcag aaggcattag tttagccgct ccacgcagcc
    21181 ccacggcctc cagcttcatc atcaccgtac tatgcaacaa tggcgcgggt agttgggtat
    21241 gttcgcgcag tggataggcg acgttgtcaa atacgttcat atcagtgaac aacgccccgg
    21301 actgaaataa catgctcatc cgtttgcgca ctgtatacag gcgcgaacga gacatcgccg
    21361 gaatattctc accatcgaaa aggatctcac catgatctgg tgcgatttgc ccgccaatca
    21421 gacggagtag cgtcgtttta ccgatgcccg atggccccat gatcgccgtg atcttccctc
    21481 gcggcacggt cagggaaata ttatcgaaga tgcagcgatt gccacgcgta aaactgacat
    21541 cgcgcatatc gactaaattc gccacagact gctccataat tcacccttcg tcttgcgttg
    21601 attttctaag catggcgctc aatttaacct tgaacccaac atatttacag aatattaccc
    21661 gccgtggtta gcgaaagctg gcatttgttt tactttttag ccgcataaag tcaaaattaa
    21721 gaatccgtta cggctttctg aaaatcttca gcggaccgac gagtatacct gaagaaagga
    21781 cgttagatgc ttttagctac ggcactgtta attgttggtt tacttttggt cgtttacagt
    21841 gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa cctttggcat cccgccgctg
    21901 atcatcggca tgacggtggt cagtattggt acatcgttac cagaaatcat cgtctcgctt
    21961 gccgcgtctc tgcacgaaca acgcgattta gccgttggta cagccctcgg ctcaaacatt
    22021 atcaatattt tgctgatcct cggtctggct gcgctggttc gtccttttac cgtccattcc
    22081 gatgttctgc gtcgtgaatt acccttaatg ttgttggtca gcgtagtggc cggttccgta
    22141 ctctatgacg gacaacttag tcgcagcgat ggtatctttc tcctctttct ggctgtgcta
    22201 tggctgctgt tcattgttaa acttgcacgt caggctgaac gtcaggggac tgacagcctg
    22261 accagagagc agcttgcaga gctgccgcgt gacggcggat tgcccgtcgc gtttttatgg
    22321 ctcggcattg cgcttatcat catgccagtg gccacgcgga tggtggttga taacgccacg
    22381 gtgctggcga attactttgc catcagcgag ttgacgatgg gtctgacggc aattgctatc
    22441 ggaaccagcc tgccggaact ggcaaccgca atagcggggg ttcgcaaagg tgaaaacgac
    22501 attgctgtcg gaaatatcat tggcgcaaac atttttaata ttgtcatcgt gttgggttta
    22561 cccgcgctga taacgccagg agagattgag ccattggcgt acagtcgtga ctacagcgtg
    22621 atgttgctgg tgagcattat ttttgcgttg ctgtgctggc ggcgctcccc gcaaccgggc
    22681 cgtggtgtag gggtattatt aactggcgga tttatcgtat ggctggcgat gttgtactgg
    22741 ttatcgccaa tactcgttga ataactggaa acgcattatg tcgcacgtag agttacaacc
    22801 gggttttgac tttcagcaag caggtaaaga agtcctggcg attgaacgtg aatgcctggc
    22861 ggagcttgat caatacatca atcagaattt cacgcttgcc tgtgaaaaga tgttctggtg
    22921 taaagggaaa gttgtcgtca tggggatggg gaaatcgggg cacatcgggc gcaaaatggc
    22981 cgcaacgttt gccagcaccg gtacaccttc atttttcgtc catcctggtg aagccgcgca
    23041 tggtgattta ggcatggtca ccccacagga tgtggtgatt gctatctcta actcaggtga
    23101 atccagcgaa atcacggcct taattccagt gcttaagcgt cttcacgtac cgttaatctg
    23161 catcaccggt cgcccggaga gcagcatggc gcgcgccgca gatgtgcatc tgtgtgttaa
    23221 agtagcgaaa gaagcctgtc cgttagggct ggcaccgacc agcagcacca ccgccacgct
    23281 ggttatgggc gatgccctcg ctgtcgcgct gttaaaagca cgcggcttta ctgctgaaga
    23341 ttttgcgctc tcacacccag gcggcgcact gggtcgtaaa cttctgctgc gcgtaaacga
    23401 tattatgcat acgggcgatg agatcccgca tgttaagaaa acggccagtc tgcgtgacgc
    23461 gttgctggaa gttacccgca aaaatcttgg tatgactgtc atttgcgatg acaatatgat
    23521 gattgaaggc atctttaccg acggtgattt acgccgtgtc ttcgatatgg gcgtggatgt
    23581 tcgtcagtta agtattgccg atgtgatgac gccgggggga atacgtgtgc gccctggcat
    23641 tctggccgtt gaggcactga acttaatgca gtcccgccat atcacctccg tgatggttgc
    23701 cgatggcgac catttactcg gagtgttaca tatgcatgat ttactgcgtg caggcgtagt
    23761 gtaaagattc aaggataaac aacaatgagc aaagcaggtg cgtcgcttgc gacctgttac
    23821 ggccctgtca gcgccgacgt tatagcaaaa gcagagaaca ttcgtctgct gatcctcgat
    23881 gtcgatggcg tactgtcaga tggcctgatt tatatgggca ataatggcga agagctgaaa
    23941 gcgttcaatg ttcgtgacgg ttatggcatt cgttgtgcgc tcacctctga tattgaagtc
    24001 gctatcatta ccgggcgaaa ggctaaactg gtagaagatc gttgtgccac attggggatc
    24061 actcacttgt atcaggggca gtcaaacaaa ctgatcgcct ttagcgatct gctggaaaaa
    24121 ctggcgattg ccccggaaaa tgtggcttat gtcggcgatg atctcatcga ctggccggta
    24181 atggaaaaag tgggtttaag cgtcgccgtg gccgatgcgc atccactgtt gatcccgcgc
    24241 gccgattacg tgacgcgcat tgctggcggt cgtggcgcag tgcgcgaagt ttgcgactta
    24301 ttactcctgg cgcagggcaa actggatgaa gccaaagggc aatcgatatg agtaaagcca
    24361 gacgttgggt tatcattgtg ctatcactgg cggttctggt gatgatcggc attgatatgg
    24421 ccgaaaaaga cgataccgcc caggtggtcg tcaacaacaa tgatcccacc tataaaagcg
    24481 agcatacgga cacgctcgtc tataacccag aaggggcact aagctatcga ttgattgctc
    24541 aacacgttga atattattcc gatcaggccg tttcgtggtt tacgcagccg gtacttacca
    24601 cgtttgataa ggataaaatc ccgacatggt ccgtaaaagc agataaagcc aagctgacca
    24661 atgaccggat gctctattta tatggacacg ttgaagtcaa cgcacttgtg ccaaactctc
    24721 aacttcgcag aatcacgacg gataacgcgc agatcaatct ggtgacgcag gatgttacct
    24781 ctgaagacct cgtcacgtta tacggaacaa catttaactc cagcggtctg aaaatgcgcg
    24841 gcaacttacg cagcaagaac gccgagctga ttgaaaaggt tagaacatcc tatgaaattc
    24901 aaaacaaaca aactcagcct taatcttgtg cttgccagct cacttctggc cgccagcatt
    24961 ccggcatttg ccgtaaccgg agacactgat cagccgatcc acattgaatc ggaccagcaa
    25021 tctcttgata tgcaaggcaa cgtggttacc tttaccggta atgtcatcgt cacccagggc
    25081 accatcaaaa ttaatgccga caaagtggtc gttacccgtc cgggcggcga acaaggtaaa
    25141 gaagtgattg acggctacgg taaaccggca acgttctacc agatgcagga caacggtaaa
    25201 cccgttgaag gtcacgcttc ccagatgcac tacgaactgg caaaagattt tgtcgttctg
    25261 acgggtaatg cttatctgca gcaggtcgat agcaacatta agggcgataa gatcacttac
    25321 ctggtaaaag agcagaaaat gcaggctttc agcgacaaag gcaagcgcgt aaccaccgtt
    25381 ctggtgccgt cgcagctgca ggacaaaaac aacaaaggcc agaccccggc acagaagaag
    25441 ggtaattaat tcgttatggc aacattaact gcaaagaacc ttgcaaaagc ctataaaggc
    25501 cgtcgcgtgg tagaagacgt cagcctgacc gtcaactccg gggaaattgt cggtctgctg
    25561 gggccaaacg gtgccggtaa gaccaccact ttctacatgg ttgtaggcat tgtgccgcgc
    25621 gatgcgggca acatcattat tgatgatgac gatatcagtc tgctgcctct gcatgcacgc
    25681 gcgcgccgcg gtatcggcta tctgccacag gaagcctcca ttttccgtcg cctcagcgtt
    25741 tacgataacc tgatggcggt actgcaaatt cgtgacgact tgtctgctga acaacgtgaa
    25801 gaccgcgcga acgagctgat ggaagagttt cacattgagc acctgcgtga cagcatgggg
    25861 cagtcactct ccgggggtga acgtcgccgt gtagaaattg cccgcgcact ggctgcgaat
    25921 ccgaaattta ttctgctcga cgaaccgttt gccggggttg acccgatctc ggttatcgac
    25981 attaaacgca tcattgagca cctgcgcgac agcggcctgg gcgtgctgat cactgaccac
    26041 aacgtgcgtg aaacactggc ggtttgtgaa cgcgcttata tcgtcagtca ggggcatttg
    26101 atcgcccacg gcacgcctac agaaatctta caagacgaac acgttaagcg tgtatacctt
    26161 ggggaagact tcagactctg atagggtaga agtttgcgac gttttagcag gagagtacga
    26221 ttctgaacat gaagcaaggt ttgcaactca ggcttagcca acaactggcg atgacgccac
    26281 agctccaaca ggcaattcgt ctgttgcagt tgtcgacgct ggaacttcag caggagctac
    26341 agcaggcgct ggagagtaat ccgctgcttg agcaaatcga cactcatgaa gaaatcgaca
    26401 cccgcgaaac gcaagacagt gaaacgctgg acaccgccga cgcgctcgaa caaaaagaga
    26461 tgccggaaga gctgccgctc gatgccagtt gggacaccat ttacaccgct ggtacaccat
    26521 ccggcaccag cggtgactac attgacgatg agctgccggt ctatcagggc gaaacgacgc
    26581 agaccttgca ggattacctg atgtggcagg tcgagctgac accgttttcc gacactgacc
    26641 gcgctattgc tacctctatc gtcgatgccg ttgatgacat cggttatctg actgtcccgc
    26701 tggaagatat tctcgaaagt atgggcgatg aagagatcga catcgacgag gttgaagccg
    26761 tccttaagcg gatccaacgg tttgatccgg tcggtgtagc ggcaaaagat ctgcgtgact
    26821 gcctgctaat ccaactctcc caattcgata aaaccacgcc gtggctggaa gaggccagac
    26881 tgatcattag cgatcatctc gatctgttag ccaatcacga cttccgcact ttaatgcgcg
    26941 tcacgcgtct gaaagaagat gtgctgaaag aagccgtcaa tctgatccag tcgctcgatc
    27001 cgcgccccgg gcagtcgatc cagactggcg aacctgagta tgtcattcca gatgtgctgg
    27061 tgcgtaagca taacggtcac tggacggtag aactcaacag tgacagcatt ccgcgtctgc
    27121 aaatcaacca gcactacgcc tcgatgtgca ataacgcgcg caacgatggt gacagccagt
    27181 ttatccgcag caatctgcag gatgccaaat ggttgatcaa gagtctggaa agccgtaacg
    27241 atacgctact gcgcgtgagt cgctgtatcg ttgaacagca gcaagccttc tttgagcaag
    27301 gtgaagaata tatgaaaccg atggtactgg ccgatatcgc ccaggctgtc gaaatgcatg
    27361 aatcgacgat atctcgcgtg accacgcaaa aatacctgca tagtccacga ggcatttttg
    27421 aactgaagta tttcttttcc agtcacatca ataccgaggg cggcggcgaa gcttcctcca
    27481 cggcgattcg tgcgctggtg aagaaattaa tcgcggcgga aaacccagcg aaaccgttga
    27541 gcgacagcaa gttaacctct ttgctgtcgg aacaaggtat catggtggca cgccgcactg
    27601 ttgcgaagta ccgagagtct ttatccattc cgccgtcaaa ccagcgtaaa cagctcgttt
    27661 gacccaaccg ataaggaaga cactatgcag ctcaacatta ccggaaataa cgtcgagatc
    27721 accgaggcac tgcgcgaatt tgttacagcc aaatttgcca aacttgagca atattttgac
    27781 cgaatcaacc aggtctatgt tgttctgaaa gtggagaaag tcacccacac ctcagatgca
    27841 acactgcatg taaacggtgg tgaaattcat gccagcgcgg aaggtcagga catgtacgct
    27901 gccattgatg gcttaattga taagctggca cgccagttga ccaaacataa agataaactg
    27961 aaacaacact aattgtccgg gcaattagca tgtgcatggc ggcctgttgt gcggcacaac
    28021 gggccatttg tacggttaat gctccgagcc tgttccactg tttgagtggg caggttctta
    28081 ggtgaaatta tgacaaataa tgatacaact ctacagctta gcagtgttct taacagggaa
    28141 tgtacgcgaa gccgcgtcca ctgtcagagc aaaaaacgcg ccctggaaat catcagcgag
    28201 ctggcggcga aacaacttag cctgccgcct caggtggttt ttgaagctat cctgacgcgg
    28261 gaaaaaatgg gcagtaccgg tatcggcaat ggtattgcca ttccgcatgg caaactggaa
    28321 gaagatactc tgcgcgccgt tggcgttttc gttcagctcg aaacgcctat agctttcgat
    28381 gccatcgaca accaaccggt ggacttactt tttgccctgc tggtgtcggc agaccaaact
    28441 aaaacgcacc tgcatactct gtcgctggtg gcgaaacgtc tggcggacaa aaccatctgc
    28501 cgccgtttac gcgcagccca gagcgatgaa gagctgtatc aaatcattac ggataccgaa
    28561 ggtactccgg atgaagcgta gttattcggt aatgtctctt ttagacgttg tgaggagaaa
    28621 cagtacatgg tactgatgat cgtcagcgga cgttcaggtt caggtaaatc tgtcgccctg
    28681 cgtgcgctgg aagatatggg tttttactgc gtggataacc ttcccgtagt gttgttaccc
    28741 gatctggctc gaaccctggc cgatcgcgag atttctgccg ccgtcagcat tgatgttcgt
    28801 aatatgccgg agtcaccaga aatattcgaa caggcgatga gtaacctgcc tgacgctttc
    28861 tcaccgcaac tcctgttcct ggatgccgac cgtaatacct taattcgtcg ttacagtgac
    28921 acgcgccgac tgcatccgct ttccagcaaa aacctgtcgc tggaaagtgc tatcgacaaa
    28981 gaaagcgatt tgctggagcc tctgcgttcg cgagcggatc tgattgtcga tacctcagaa
    29041 atgtccgttc acgagctggc tgaaatgctg cgtacccgtc tgctgggtaa acgtgaacgc
    29101 gaactgacca tggtctttga gtctttcggc ttcaaacacg gtatccctat cgatgcagat
    29161 tacgtctttg acgtgcgctt cttgccgaac ccgcactggg atccgaaact gcgtccaatg
    29221 acaggtcttg ataaacctgt cgccgcgttc ctcgaccgcc acacagaagt acacaatttt
    29281 atctaccaga cgcgaagcta tcttgagcta tggttaccta tgctggaaac caacaaccgt
    29341 agctacttga cggtcgccat tggttgtacc ggcgggaagc accgttcggt gtatattgca
    29401 gagcaactgg cagactactt ccgctcgcgc ggtaaaaacg tccagtcacg ccatcgtacg
    29461 ctggaaaaac gtaaaccatg accgtcaagc aaactgttga aatcacaaac aagctgggca
    29521 tgcatgcccg gcctgcaatg aagctgtttg aattaatgca gggttttgac gctgaagtgc
    29581 tgttacgtaa tgacgaaggc accgaggccg aagccaacag cgttattgcg ctgctgatgt
    29641 tggattctgc caaaggacgg cagattgaag ttgaagcgac cggcccacag gaagaggaag
    29701 cactggccgc cgttatcgcc ctctttaact ctggttttga tgaagattaa tcttcatcac
    29761 tttgacatac aactcccttc aaactccccc cgacaataag aaaatcacgt actgaaatcg
    29821 ttctcaatca acgtcatttg tacattttgt gcgcttttca ctttcagaag aaccttaaga
    29881 aaaccttaag aggcattgtt taggttttgt ttaagttaat cgaccatact ggagatcgtc
    29941 agaaaatatt tccaggagat ggcatgattc gcttatctga acaaagtccc ctgggcaccg
    30001 gaagacatcg gaaatgttat gcgcatccgg aagatgccca acgctgtatc aagattgtct
    30061 accatcgtgg cgatggtggc gataaagaga tccgccgtga gttaaagtac tacgcgcatc
    30121 ttggtcgccg gttaaaagac tggagtggaa taccgcgcta tcacggtacc gttgagacgg
    30181 attgcggaac cggttatgtc tacgatgtga ttgcagattt tgacggcaaa ctctccatta
    30241 cgctgaccga atttgctgaa caatgccgtt acgaagaaga cattgcacaa ctgcgccagc
    30301 tactgaaaca gctgaagcgt tatttgcagg ataaccgtat cgtgacgatg tcgctgaagc
    30361 cgcagaatat cctctgtcac cgcatcagtg agtcagaggt tatcccggtc gtctgcgaca
    30421 acattggtga aagcacattg attccactgg cgacctggtc gaagtggtgt tgtctgcgta
    30481 agcaagagcg gttatggaaa cgatttatcg cccaaccggc gctggctatt gccctgcaga
    30541 aagatctgca acctcgtgag agcaaaacgc tggccctcac ttcgcgcgaa gcttaatcca
    30601 gctggtgttg ttgcataaac ggctcaccgc ctaactgata catctgccgt aaaatccacg
    30661 cctgacggct acgcacgtag cccgatggcg aggagacttt gaaacgaagt ggattaggta
    30721 atacagctgc cagtaatgca gcttccgacc gggtaagttt gctcgcgggt ttgtggaaat
    30781 aacgttgtgc cgcagcttcg acgccaaaca caccgtcgcc aaattcggcg atattcaggt
    30841 aaaccgtcag gatacgcttt ttgctccaga ctgtttctat ccccagcgtt aatccagctt
    30901 ccagcccttt tcgtacccag ctacgcccat cccataaaaa gagatttttg gccgtctgct
    30961 gagaaatcgt tgaagcaccg cgaatacggt tttcattgcg ctcgttgtgc gccagggctt
    31021 tctcaatgga agcgacatca aagccccagt gctcagaaaa tttctgatct tctgcggcaa
    31081 tcaccgccag tcccatccac ggcgagattt gatccatact gacccagtca gaatgtgcca
    31141 cgtaacgaaa attgccatgc agccaggcgc tgacctgtcg ctcgaccatt accgctgaga
    31201 agggaacagg cgcaacacta aacaacgcga tgcccccgcc ccagaaaacg gcgaggacaa
    31261 ccattaacag caaaagaaaa cggcgaacga aactaaacac cgttaagcgg cttttactca
    31321 ttcagccaga accagcacgc gggaaaccag cttatcaatg ccgctcgccg cttctgcaat
    31381 gttctgcgcc agcatatatg ctggggtggt gacaatctta ttgtcttcat caaccacgat
    31441 atcatcgaca ggacagggca catgctccgc gcccatctct tccagcactt ctgcggtatc
    31501 gatatcagta ccgatggtca aacgcagcgg gaaatcgaaa attttcggca gcatcgccgg
    31561 ggcaatacac ataaaaccaa gcggttttcc ggcctgatgc atcgcttgtg ccagcgcctt
    31621 taattcacgg tcaacggtgc attcgctacc aagagtggca aaattgctta aattcttcgc
    31681 cgcgccaaac ccccccggca caatcaacgc atccagttca gcggcatcgg cctgggccag
    31741 aggacggatt tcaccgcgcg ttattcgtgc cgcttcaatc agcacgttgc gcgtttccgt
    31801 catcgcttcg ccagttaaat ggttgataac atcaacctgc tgcttatccg gtgcaaagca
    31861 gaccgcctgc gcaccgctgc gtgaaatagc taacaacgtc aacaccgctt catgaatttc
    31921 agaaccgtca tagacgccgc atccgctcag aattacgcca attttcttca ttgtgatcat
    31981 cctttcgcaa ttgactgaaa cacattaatt tttttaataa aaatggtacg catcacacat
    32041 ttaactgatt catgtaacaa atcatttaag ttttgctatc ttaactgcgt gcggcctgaa
    32101 aaacagtgct gtgcccttgt aactcatcat aataatttac ggcgcagcca agatttccct
    32161 ggtgttggcg cagtattcgc gcaccccggt ctagccgggg tcatttttta gtggcttttg
    32221 ccacccacgc tttcagcact tctacgtcgt gacgccactc ttctttcatc tcttcaatcc
    32281 attcaccgac gttatcttcc caggcaggaa ggtcaggaga ctgaatttgc tgacccagct
    32341 gttgcagatg gcgtaacccc actgaccccg ccgcaccttt aattttatgt ccttcctcaa
    32401 caatgccttt tttatcctgc gccgtcagat tcgactccag cacgctgaca tagcccggca
    32461 tcatcttctc aaacaccgct aacccgtcgg tgatcagctt cggtcctaca agttcgagat
    32521 actgttccag catgggaata tcgagcaatg cttctgattt actgttctct tctgtcgtca
    32581 ccgtactctc ctcatcatcc tgggtatccc agaatttctt gatcatcgcg gttagcgccg
    32641 gaacagaaag cggcttactc agcacatcat ccattccagc attgaggtac tcttgtttgt
    32701 ctttcagcac gttagcggtt aaggccacca gcggcggtaa atcctctcgc ggataacgct
    32761 tcgtcagttc acgagagata tccagcccgg tcatatctgg caactgaata tccagtaaca
    32821 ccaggtcgta ttcgcccggt ttaaacatct ctagcgccgc cttgccggtc atggcgacat
    32881 caacgctgtt acctaatttt tccagcacag aacgcgcaac aatcacgttc agttcaatgt
    32941 cttccaccag cagcacattc agcgccggta aaggcatatc gtcttcatca aacgcatcat
    33001 cgacctcttc tgctaccgac ggtgcgtgga tcgtcaacgt aaaggttgaa cctttgccct
    33061 gttcgctggt aaccgtaata tcgccgccca tatttttcgc cagacgacga gaaacggcca
    33121 gaccaatacc ggtgccggtg gcaggtttac cgccatgact gtctttcacc tggtaataca
    33181 tggcgaaaat tttatccagc tcatcctgcg gaatgccgat accagagtct tccacttcaa
    33241 aatgcagcat atcgccttca tcgtagcgca cgcgcacggt aacctggcct tgctgggtga
    33301 atttgacggc gttactgatg aggttccaca ggatctgccg taaacgcgtc ccgtcggtaa
    33361 tgacctgatg cggtaatggc agcgtcggct ccaggttaaa gcgcaatcct ttttgttgcg
    33421 cctgcaatgc ggagagattt tccagatcgg caaggaagct ggtgaaatca accggttgat
    33481 tatcaagctg gaccttgcgc cgttccatct tatccatgtc gataatatcg ttaaagatat
    33541 tccccagcgt gacggccgaa acatggatgg tcttgagata tttttcctgc tcggcggtga
    33601 gttcggtatc cagcagaatg cggctcagac cgacgatacc gttcagcggt gtacgcaatt
    33661 cgtgactgat ggtggagata aacgtcgttt tgtcgcggct ggcccgttca agcgcatcct
    33721 gataccgctt acgctcggta atgtctcgac caaagcccat caaaccgtga cgtttaccca
    33781 cgcggtcgta gtacggcact ttacggattt caaagcaggc tttgcgcccg tccgggtaat
    33841 ccagccactg ttcataggtc agtgacacat tatgacggaa cactttttca tcggtttcaa
    33901 tgacttttgc ggcggcttcc ggtgagtaaa catcaacagg tttcaggtga accagttgtt
    33961 tttcgctttt tccggtcagc agctccatcg cgcggttaca gccggaaaac tctttatctt
    34021 cgttacgata aaaaaccagg tcgggtgaag catcaaggaa ggaacgtaag aatgaggatt
    34081 gctgctcgag ctgaatttgt gtctcttcgc gctctttgat ttcaattttc agttggccga
    34141 aggtttcctg cagttctgct tccgcttttt cacgaacggc gatttcctga tttagctggg
    34201 caatattatc ttttaactga acgttgaggc tcaaatcgcg ctcgcgcatc tcctccagtt
    34261 tttgcaccag ccgtgacaga cgttgtcgtg actcctccag ttgctcgacg accaccgata
    34321 gaaagtagac cgcccacggc gtaatcaaca aaccaaagaa gatagaacga ataacatcaa
    34381 tgctttcgac ctgaccatgc agcaccatgg ttaccgccat ttgtaccaca atggcaagaa
    34441 cgacgagggc cagcgccagc aacattgaga agcgcaccag acctaacttc atcatcaggt
    34501 caacataata ctgcgccagc agacgaattt gcttcattag ggaattcctt cacgacaacc
    34561 tgccccaata atacccaatt ctgacagtta cgttataaat tgtgtgagaa atgcggaatt
    34621 cccctcaacg acctactccg ttggaggtag ccacggacgt cccagcgccg atccctgtac
    34681 gccatgttca ttcagatagc gatccagctc gaccattccc gtccatcgat tttcgcacca
    34741 cagaggagca agcagcgtcg ggcgacgggc gctggcggaa atgcggtggt agatcacttc
    34801 cggtggcgta tggcgaatca tttctccggc agtgaacgtg taatcctcca gttcaatacc
    34861 gttcaaacgc cccgcttccc aggctttcgc cataatgctg cctttcacaa tatgcaacgg
    34921 atgtagcttt ataccgtcaa caccggtttc cactacccgc tccagcgttt gcagacattc
    34981 agccagccct tcgcctggca gaccgacaat taaatgcgag cacaccttca gcccgcgctg
    35041 gcgtgccagc tgcgtcgtgc gctgataaca ggcgaaatca tgaccgcgat taatgcgatg
    35101 tagcgttttg tcgtgcacgg tttgcagccc cagctccagc cacacttcgt agccctggtc
    35161 tttatattcg caaagcagat ccagcaccgc atccggcacg cagtccgggc gcgtgccgac
    35221 acacaaaccg acaatattgg cctggctcac cgcctgttga tacatcgaac gcagcacctg
    35281 aacttcggca aaggtgctgg tatacgcctg gaaataggcg aggtaacgtt tcgcgcgatt
    35341 aattaaattc gcctgatgcg ccagctgttc ggcaatggaa cgatgctgct gcgcttcgtc
    35401 ggcaaacgag gcaacattac agaaggtgca gccgccgcgc ccgatggtgc cgtcgcgatt
    35461 cgggcaactg aaaccgccgt gcagcgtcag cttatgcacc ttttgcccat aacgacgggt
    35521 gagatcacca ccaaacatat tgactaattt ctgtaactgc ataatctgat agaccgcgcc
    35581 ttgaaaagag gccaaagcct gccattttta gctcaattcg gcgatgacct ggatcaatcg
    35641 tcccggcccg cttttatcaa ctgcataatc aatcaaaatt accgaaattt catgcataat
    35701 cacataaatc acttttgctt atcttgtgtc agattttttt atctccttat ggattttagg
    35761 caaaaacagt agcatgaaac gtcattacca attaatgcag tataaaatgc tggttttctc
    35821 gtcaattcaa ggcaggataa gggttaacac gcctttatga cagtcaggaa ttgactgttt
    35881 ctctaacgac ttccctttta gccttaaaga taaaatctat tttaatttca gccatttaat
    35941 aaagaatttt gcgctaaagc acatttctgt accaataagc ttgccatttg acctgtatca
    36001 gctttcccga taagttggaa atccgctgga agctttctgg atgagcagcc tgctcatcat
    36061 atttatgcag taattgagat cccctcttca ccgtattaac cgatgcgaaa aggacaacaa
    36121 gggggcgaat gcgaggcgcg cgtatgacac gcaacccccg tcgccacgct ctttctgtac
    36181 ccgtgcgcag cggttcggta gtggggttcc cgcagagcct gggggaggtt cacgatatgt
    36241 tgtacgataa atcccttgag agggataact gtggtttcgg cctgatcgcc cacatagaag
    36301 gcgaacctag ccacaaggta gtgcgtactg caatacacgc actggcccgc atgcagcacc
    36361 gtggcgcgat tctcgccgat ggtaaaaccg gtgacggttg cggcttgctg ttacaaaaac
    36421 cggatcgctt ttttcgcatc gttgcgcagg agcgcggctg gcgtttagcg aaaaactacg
    36481 ccgtcgggat gctattcctg aataaagatc ctgaactcgc cgctgccgca cgccgcatcg
    36541 ttgaagaaga actgcaacgc gaaaccttgt cgattgtggg ctggcgtgat gtccccacta
    36601 acgaaggcgt actgggtgaa atcgccctct cctctctgcc ccgcattgag caaatttttg
    36661 tgaacgcccc ggcaggctgg cgtccgcgcg atatggagcg ccgtctgttt atcgcccgcc
    36721 gccgcattga aaagcgtctc gaagccgaca aagacttcta cgtctgtagc ctatcgaatc
    36781 tggtgaacat ctataaaggt ctgtgtatgc cggcggatct gccgcgcttt tatctggatc
    36841 ttgcggacct gcgtctggaa tcggccattt gcctgttcca ccagcgcttc tccactaaca
    36901 ccgtaccgcg ctggccgctg gcgcaaccgt tccgctatct ggcgcataac ggtgaaatca
    36961 acaccatcac tggtaaccgc caatgggcgc gtgcgcgtac ctataaattc cagacgccgc
    37021 ttatccctga tctgcacgac gccgcaccgt tcgtcaacga aaccggctct gactccagtt
    37081 cgatggataa catgctggaa ctcctgctgg caggcgggat ggatatcatc cgcgccatgc
    37141 gtctgttagt gccacccgcc tggcagaaca acccggatat ggacccggaa ctgcgtgcct
    37201 tctttgactt taactccatg catatggagc cgtgggatgg cccggcgggc atcgtgatgt
    37261 ccgacggtcg ttttgccgcc tgtaacctcg accgtaacgg tctgcgtccg gcgcgctacg
    37321 tcatcaccaa agataagctc atcacctgcg cctctgaagt cggtatctgg gattaccagc
    37381 ctgacgaagt ggtcgaaaaa ggccgcgtcg ggccaggcga actgatggtt atcgacaccc
    37441 gcagtgggca tattctgcac tcggcagaaa ccgatgacga tctgaaaagc cgccatccat
    37501 ataaagagtg gatggagaaa aacgtccgcc gactggtacc gtttgaagat ctgcccgatg
    37561 aagaagtggg tagccgcgaa ctggacgacg acacgcttgc cagctaccag aaacagttta
    37621 actacagcgc ggaagagctg gactccgtaa ttcgcgtact gggcgaaaac ggtcaggaag
    37681 cggtcggttc gatgggcgat gataccccat tcgccgtgct ctccagtcag ccgcgcatta
    37741 tttacgacta cttccgccag cagtttgccc aggtgactaa cccgccaatc gacccgctgc
    37801 gtgaagcgca tgttatgtcg ctcgccacca gtatcggtcg tgaaatgaac gtcttttgcg
    37861 aagcagaggg ccaggcgcac cgtttaagct ttaaatcgcc gattctgctc tactccgatt
    37921 tcaaacagct cacgacgatg aaagaggagc actaccgcgc agatacgctg gatatcacct
    37981 ttgacgtcac taaaaccacg ctcgaagcga cagtcaaaga gctgtgcgac aaagccgaaa
    38041 aaatggtacg tagcggcacc gtgctgctgg tgctctccga ccggaatatc gctaaagatc
    38101 gcctgccggt tccagccccg atggcggttg gcgcgatcca gacccgtctg gtcgatcaaa
    38161 gcctgcgttg cgatgccaac atcatcgtcg aaaccgccag cgcccgcgat ccgcaccact
    38221 tcgccgtgtt gctgggcttc ggcgcgacgg ctatttatcc ataccttgcc tatgaaacgc
    38281 tgggccgcct ggtagacacc catgcgattg ccaaagatta tcgtaccgtg atgctcaact
    38341 accgtaacgg catcaacaaa ggcttgtaca aaatcatgtc caaaatgggc atctccacca
    38401 tcgcctctta ccgctgctcg aaactgtttg aagcggtcgg tctacacgat gatgtagtgg
    38461 gcctgtgctt ccagggggcg gtcagccgca ttggtggggc aagctttgaa gacttccagc
    38521 aggatctgct gaatctgtcg aaacgtgcct ggctggcgcg taagcccatc agccagggcg
    38581 gcctgctgaa atacgtccac ggcggcgaat accacgccta caacccggac gtggtgcgca
    38641 cgctgcaaca agcggtacaa agcggcgagt acagcgacta tcaggaatac gcgaagctgg
    38701 ttaatgagcg tccggcaacc acgctgcgcg atctgctggc aattacgccg ggtgaaaacg
    38761 cggtcaacat tgctgatgtt gaaccggcaa gcgaactgtt taaacgcttc gataccgctg
    38821 cgatgtctat cggcgcgtta agcccggaag cccacgaggc gctggcggaa gcgatgaaca
    38881 gcatcggcgg taactcaaac tccggtgaag gcggtgaaga tccggcgcgc tacggcacca
    38941 acaaagtgtc gcgcatcaag caggtggctt ccggtcgctt tggggttact ccggcgtatc
    39001 tggtcaatgc cgacgtcatt cagattaaag tcgcccaggg cgcgaagccg ggcgaaggcg
    39061 gtcagttgcc gggtgataaa gtcactcctt acatcgccaa actgcgctat tcggtgcccg
    39121 gagtgacgct gatctccccg ccgccgcacc acgatatcta ctctatcgag gacttagcgc
    39181 agctcatttt cgacctcaag caggttaacc cgaaagcgat gatctccgtg aagctggttt
    39241 ccgaaccggg cgtaggcacc atcgcgactg gcgtggcaaa agcttatgcc gatttaatca
    39301 ccatcgcagg ctatgacggt ggcaccggcg caagcccgct gtcgtcagtg aaatacgcag
    39361 gttgcccgtg ggagctgggg cttgttgaaa cccagcaggc gctggttgcc aatggtctgc
    39421 gtcataagat ccgtttgcag gtcgatggcg gcctgaaaac cggtgttgat atcatcaaag
    39481 cggcgattct cggcgcagaa agcttcggct tcggcactgg cccaatggtc gcgctcggct
    39541 gtaaatatct gcgtatttgc cacctgaaca actgcgcaac gggtgtggca actcaggatg
    39601 acaaactgcg taagaatcac tatcacggcc tgccgttcaa ggtgacgaat tactttgagt
    39661 ttatcgcccg tgaaacccgc gagctgatgg cacagctggg ggtaacgcgg ctggtggatc
    39721 tgattggtcg caccgacctg ctgaaagagc tggacggttt caccgccaaa cagcagaaac
    39781 tggcgctgtc gaagctgctg gagaccgccg aaccgcatcc aggtaaggca ctctactgca
    39841 ccgaaaacaa cccgccgttt gataacggcc tgctgaacgc gcagttgctg caacaggcga
    39901 aaccgtttgt cgatgaacgc cagagcaaaa ccttctggtt cgatatccgc aataccgatc
    39961 gctccgtggg cgcgtcgctt tcaggctata tcgcccagac gcacggcgat caggggctgg
    40021 cagccgatcc tatcaaagcg tacttcaacg gcaccgcagg ccagagtttc ggtgtatgga
    40081 acgcgggcgg cgtggaactg tacctgaccg gggatgccaa cgactatgtc ggtaaaggca
    40141 tggcgggcgg cttaatcgcc attcgtcctc cggttggttc cgccttccgc agccatgaag
    40201 caagtattat cggcaacacc tgcctgtatg gcgcgaccgg tggtcgtctg tatgccgcag
    40261 gccgcgcggg tgaacgtttc ggcgtgcgga actccggtgc tatcaccgtg gtagaaggca
    40321 ttggcgacaa cggctgtgaa tatatgacgg gcggtattgt ctgtattctg ggtaaaaccg
    40381 gcgttaactt cggtgcgggc atgaccggcg gcttcgctta cgttctcgat gaaagcggtg
    40441 atttccgcaa acgcgttaac ccggaactgg tcgaggtctt aagcgttgac gatctggcga
    40501 tccatgaaga gcatctgcgc ggtcttatca ccgagcatgt tcagcatact ggctctcagc
    40561 gcggtgaaga gattctggcg aactggtcaa ccttcgccac taaatttgcg ctggttaaac
    40621 cgaagtccag tgatgtaaaa gcactgctgg gtcatcgtag tcgtagcgca gcagagttgc
    40681 gcgtgcaggc gcagtaaggg gtagcaacaa tgagtcagaa tgtttatcaa tttatcgacc
    40741 tgcagcgcgt tgatccgcca aagaaaccgc tgaagatccg caaaattgag tttgttgaaa
    40801 tttacgagcc gttttccgaa ggccaggcca aagcgcaggc tgaccgctgc ctgtcgtgcg
    40861 gcaacccata ctgcgagtgg aaatgtccgg tacacaacta catcccgaac tggctgaagc
    40921 tcgccaacga ggggcgtatt tttgaagcgg cggaactgtc gcaccagacc aacaccctgc
    40981 cggaagtttg cggacgagtc tgcccgcaag accgtctgtg cgaaggttcc tgcactctga
    41041 acgatgagtt tggcgcggtg accatcggca acattgagcg ttatatcaat gataaagcgt
    41101 tcgaaatggg ctggcgtccg gatatgtccg gcgtgaagca aaccggtaaa aaagtggcga
    41161 ttatcggcgc aggcccggca ggtctggcgt gtgcggatgt cctgacgcgt aacggcgtaa
    41221 aagccgttgt cttcgaccgt catccagaaa ttggcgggct gctgaccttc ggtattccgg
    41281 ccttcaagct ggaaaaagag gtaatgacgc gtcgccgtga aatcttcacc ggcatgggta
    41341 ttgaattcaa actcaatacc gaagtgggcc gcgacgtgca gctggacgat ctgctgagtg
    41401 attacgatgc cgtcttcctt ggcgtcggga cttatcagtc aatgcgcggc gggctggaaa
    41461 acgaagacgc cgatggcgtg tacgcagcgc tgccgttcct tatcgccaac accaaacagt
    41521 taatgggctt tggtgaaacc cgcgacgaac cgttcgtcag catggaaggc aaacgcgtgg
    41581 tggtccttgg cggtggcgac actgcgatgg actgcgtgcg tacgtccgtg cgccagggag
    41641 cgaagcacgt tacctgtgcc tatcgtcgtg atgaagagaa catgccgggt tcccgccgcg
    41701 aagtgaaaaa cgcgcgggaa gaaggcgtag agttcaaatt caacgtccag ccactgggga
    41761 ttgaagtgaa cggtaacggc aaagtcagcg gcgtaaaaat ggtacgtacc gaaatgggcg
    41821 aaccggatgc caaaggccgt cgccgcgcgg agatcgttgc aggttccgaa catatcgttc
    41881 cggcagatgc agtgatcatg gcgtttggtt tccgtccaca caacatggaa tggctggcaa
    41941 aacacagcgt cgagctggat tcacaaggcc gcatcatcgc cccggaaggc agcgacaacg
    42001 ccttccagac cagcaacccg aaaatctttg ctggcggcga tatcgtccgt ggttccgatc
    42061 tggtggtaac cgctattgcc gaaggtcgta aggcggcaga cggtattatg aactggctgg
    42121 aagtttaagc tctgtcccag cgccgcctcc tcagaaaaga gtcggcgctg aaattctcct
    42181 ttctttctgg aaagcgcctc gcataatctg aagaaaacca atgtaatacg ctcatctgga
    42241 ttgttaccgg tagatgcccg gcgtatagct gattccatta ttcaacgcag cgccatataa
    42301 agggacagat aatggaatcg ctctcagaga gaacctcaac aggctaccag caaatccacg
    42361 acggtattat tcacctggtc gatagcgccc ggacggaaac ggtacgtagc gttaacgcgc
    42421 tgatgaccgc gacatactgg gaaattggcc gacgaattgt cgaatttgaa caaggtggcg
    42481 aggcaagggc tgcgtatggt gcgcagctaa tcaagcgact atcaaaggat ttaagtctaa
    42541 ggtataagcg tggtttctct gcaaaaaact tacgccaaat gaggcttttt tacctctttt
    42601 ttcaacatgt tgaaattcgc cagacagtgt ctggcgaatt aacaccattg ggaattccgc
    42661 agacaccatc tgcagaattc ccatcggcaa agatttggca gacgctgtct gccaaattga
    42721 gaggctgttt gatatttcca cagcttttgt attgcatctc ctattttcgg tttgtatatt
    42781 catgcaaata aacgaaggaa agttgccgtg atttacattg aacagcttga attaatccat
    42841 aaaagtggtg atgtgttgta tccagtaaaa ataaccagaa aatcatcagg aaaaactgcc
    42901 ttccaccttg ttccattcgg cttaaataag actcacgacc ttctagaggt ggaagatgct
    42961 tcagaagcaa ttcggctggt gattgatgaa cgtcattcca ttcgctgcag tacacttaca
    43021 gcaactatta cgaataaaaa gggcaagcga attaagcgga caggtatata cagtatcaag
    43081 ggtgttaaca taaaagaata caacgtcagg taatggcaca gagcggtccg tcagttcagg
    43141 tttggctctg tgccaaagat gggtcaggtc acacctgatc taataaattt aagccattaa
    43201 atcagcctta accttcacca ccaccttttt aatctcaccc ggctcgccaa caacgcatcc
    43261 cggcttatgc ggctctccta gcataaacac ggcgaacatt cccggtttca ggatgatggc
    43321 ttgctcgttt tcaatggtgc tgcaaagctg gtaatcatcc tcatggtgga actcttcaca
    43381 ctgacgcgca gtgcctgcca tgccaaacag aatccgttct tcaccgttta ataacagctg
    43441 gatatcaatg tattgctcgt gcagctccgc ttttttctca acgggcgatt gagtgttaaa
    43501 cgtcatgaca ttcataaaaa tattgtcgcc ctgtaattcg taacgacccg gcgctttttc
    43561 ttgcggtctg gcagctaatg ccagcgttaa cgcgtcctgt aacgcaggat gtaacccagc
    43621 agacggtaat gaccgtactt cacccatcat cataattttt ctccctgggc caacagcgct
    43681 gccccaagta aacctgcatc atggcggtag tgcgccgcca gtaaatcaac atgaaatacc
    43741 gctggctcct gcgccagata cgtttccacc aacgccagat acccttctgc cagaccaacg
    43801 ctgccaccga ccaccacgca ctggcaatca gttgtggctt taatatcagc gatcagcctt
    43861 gccagcacat gtgcggagcg gtgaatcagt tgctgcgcct gctcgtcgcc ctgcccggcg
    43921 cgtgtgaaaa tagttctcgc atccgcgccc atgagttcac cttgcgctgc cgctgcaata
    43981 ccgcgaccag aagcaatcgc ttccacgcaa cctgtgcgtc cacagccgca gactgggccg
    44041 tgtggatcgg caagcgtatg cccgatatgc cccgccagac cgccagggcc ggtaagcagt
    44101 ttaccgccgc tcactacacc gccgccgacg ccagtggaaa cggtgataaa caccatctcg
    44161 gttacatcgc cttccagcgc ctgatactct gcccatgctg cggcctgcgc gtcgttaatg
    44221 gcaatggtcg gcaaattggt aagttgttcc agcgttttga ctaacggaaa gtgtagcaat
    44281 ccaccgagat tatgcggatt cagcgccagc aaactgccgt cgcgaataat tccggttgaa
    44341 gcgatggcaa cccgctgcgc atgagcttgc aacggagaga ctaatgcgga taaggcatca
    44401 cgcaaggctt ctggtgtctg gctggctggc gtaggaagtt cacgacgatc gcggatctgc
    44461 ccgtcagcgc caatcagcgc ggcggcaagt ttagtaccgc cgatatcaat cgccagtgtg
    44521 gtcatagcac cgcctttttc atcgctgtgt tgtaccactg acaaatgtgt tcaagacgcg
    44581 taatggcaga accgaccgtc accgcccacg cgccgtggcg catcgcatct gccgcctgag
    44641 caggcgtgtt gtaacgccct tcggcaatca cccgacatcc ggcttcgctc aacgttttca
    44701 ccagcgccag atccggctct tctggcgttt caggcgtggt atagccagaa agcgtagtgc
    44761 caataatttc ggctcccagc ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg
    44821 tcatcgccag taaaccgtga tgatgtatac gtgccagcag cgtttcaaca ggcaccggac
    44881 gcgggcggtc ggtgccgtca atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcga
    44941 catcttcaat ataggccgtg atgcgtaccg gagaatcctc cagatcgcgt ttcacaattc
    45001 caataatcgg cacgctcacc accgcacgcg tggcttgcag atttgccaca ccttcgatgc
    45061 gaatggcaac cgcgcccgcc tgctctgccg ctaatgccat ggctgcgacg atttcgggtt
    45121 tatcgagcgg gctgtcagga atcggctggc aggagacaat caggccaccg ttagcagcga
    45181 ttttttgatc cagttgtgca agtaacgaca tacatcttcc cttagcgaaa ggcccggtac
    45241 atagaccggg caacaggatt aacttttggt tttgactaaa tcgtttttgg cgctgccaaa
    45301 tggcacggca ccgctaaatg gtttgccgtc gatagcgtca tgagtacgca atgcttccgg
    45361 gcgcagccaa cgctgaacgc gggaaggcat atccagacca atcagcagga tcaccacaaa
    45421 tgtcaggctg aacgagagcg atgccagcgc agtacccaga tccagacgtt gagcgatcaa
    45481 cgcgccgatg attggggcca gtgcgccacc caatgcgcca acgttgtagg taaagcccag
    45541 gcccgctgca cgctggtcgg tatcgaaata accgccaatc agtttcggta agatcccggc
    45601 gatcccttgt ccaagcattt gctggaagaa cagtaacaga ccgagcaccc agacgtttgc
    45661 gccgccaatc gcaaataccg gaataatcag cagctgcgag gccagcaggc tacaaacgta
    45721 cgctttgcgg gttcccagcc agtcaccgag gaagcctcct acgcagcatc ccacggccgc
    45781 gccaaagcca ctaaagaaca gcacattggc gacagtatgc gggttataag ccagatcggt
    45841 tttcagatac gttggcagca gcgcctgaat cggccatgag tagaggaaag caaacaacac
    45901 gaccaccatc agcattacgc ccgttggcca gcgtttgcct gtactctgca ccataaagct
    45961 gataaagatt gcggcgcata acagcccaag aacagcgacg atcgcggcat tttgcaggtt
    46021 accggcgaag cagaaccaca gcgcagtagc cgccgccagt gtcattacga tattggcaat
    46081 gcgatgttca ccacggtaga gaatatccac cattgtgcgt actggtgctt tacctgcgtg
    46141 tttctctttc cagtcttccg cttccgggat gtttttacgc agccagagag caaagatgat
    46201 tggcaaaatg ccgataaaga acagcgcacg ccaaccccag accggaacca ccaggctata
    46261 gacctgagcg gcaacgacgg cccccacaga gaagcctgaa atcaaaaaac cactggcttt
    46321 gttacgcaga tgttttggcc agctttcaat gacataggtg gcgctggaac cgtattcacc
    46381 cgccatcccc atgccgatga ccagacgagc gataaacatg gtgatgtagc ctggcgcaaa
    46441 gccgcaggcc agcgtcccgg ccgagaagag aacgatgctg gtgaccattg ccagacgacg
    46501 cccgtagcgg tcacccatag cgccgagcat caggccgccg aaccagcgag agataaaggc
    46561 tgcggagatc agacttgccg cctgcaccgt cgtcagcccg aattcaccct gtacttcggt
    46621 gagtaccagg gcgattaaaa cgaaatcaaa accgtcaagc agatatccca accaggcagc
    46681 ggaaaatgcg cgccattgtg cacggttgag atggcgatac cacgggatat tctgggttgt
    46741 agtactcatt gtgagtctcc cgcggtgggc gatgcccaca cgctttggta tgaaaattgt
    46801 agggtacaga tgcgtttatt tcccctcacc cggtaggggc gagcgagggg aaaacaactc
    46861 acccgcgctc ttgcatcaac tgctgggcca gcgccttcag ttctggcaga tatttttcat
    46921 ctaccggtcc aaacggtttg cggcacagcg gcacagaaac gacatccata taatgcagga
    46981 cagttttcag gccgcggaat acgccagttt tgatcagtaa atcaatgact ttgttgcact
    47041 cagtttgcag tttctgcgcg gtctggatat cgccttcttt cagcgccttc acgatcccct
    47101 gatatcgcca gcccatgatg ttgtaagtac tgccgatacc accatcagcg cctgccagca
    47161 agccggaagc gaagatttcg tcgtaaccgt tatagagcac cagatcagga tgttcacgac
    47221 ggatctgttc catctgatag agatcgccag aagtctgttt cagcgcaccg acgccaggca
    47281 acgtaaccag ggtgttgatc tgatccaggg tcagttttac cccactcagg gctggaatgt
    47341 tgtacacaac catcggcaaa ccatccgctg aatcaataat tgcccgatag tgatcgcagt
    47401 gttcttcaaa gctgaaagga tagtagaacg gcgtgacggc ggagacggca tcgaagccat
    47461 aacgtttagc cgatgccgca agttgttggc tttcggcggt gctgacgcaa ccgacgtggg
    47521 cgatgagttt aatcttacct ttcgcctctt cggcgacgat ttccagtacc tgttcacgct
    47581 cggaaaggct ttgtacaaag gcctcgccgg tcgaaccacc cacgtataaa ccgtcgatgc
    47641 cctgctgaat attgaactga accaggcgac gcagactcgc tttatccagt gcttgttgtt
    47701 ggtcaaaagg agtcaggagt gcagccatta cgccacgtaa attcgttgcc ataaatacct
    47761 ctgaagtgat acttgtctga taaacgatat acctttatac ctgttatacc agatcaatta
    47821 agcaacaccc catacagaaa gcttataatg cgatctgctt cactaaagtg gcattatttc
    47881 tttttgttgg tggtctgacc gaaagcgtgc caggtagcag agacgctgtt gagatgcgat
    47941 tgcaacgcac gatcggcttc gtcaggatca tgacggcgga tcgcatcaac gatcgcaata
    48001 tgctgttgat agctaacgtt gttatgctcg tgcagagcct gatcagccac ggttggacgt
    48061 gcggcgatca gccagtcgag cagggcaacg tggatcgcca tgaagattgg gttaccaggg
    48121 atctccgcca gcacgcggtg gaaatcaacg tctgaacgaa tgaatgccgc gttgttatcc
    48181 agcgactgac tgttgatttc cagtgctttt gccagcaaat cgatttgctc atcggtggca
    48241 tgttcagccg catagcgcac cagactggat tcaaagaaca gacgtaattg ttcgaaatgg
    48301 gcaatcccac cgggatgaga aaggaaatct ttcgccatgc cggaaaactc accgatgata
    48361 gtgtccgcag aaggacgcga gacgcgagcg cgttcgccgt tgtttatttg caccagacct
    48421 ttgcgtttta acgctgccag cgcttcgcgc acagaaggac gcccgacgtt aaagaacgcc
    48481 atcagttcgc gttcagacgg taattgttca ccttcgccaa attcacgacg gcggatcatc
    48541 tgttccagct cttcttccac catttcggag agttttttac gcgccagcgg gcggctacgc
    48601 aagttgcgac caattgcagg tgaagaatct tcggtttgcg aatcaaatgc gttcataagg
    48661 cccattctgt aaggtcagtg tgattaacat catcagtgac atcctatcac aggattgaaa
    48721 gtaggggaaa atggcaggtt tttctctttg tgcctcatca ttaccataat taacggaata
    48781 attaactatt gcgaaaaatt aatgtaacgc agat
//
LOCUS       DAZPJR010000010        46684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPJR010000010 DAZPJR010000000
VERSION     DAZPJR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.011"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHVB7_000574"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHVB7_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1912663.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHVB7_000575"
     CDS             complement(280..873)
                     /locus_tag="ACHVB7_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1912664.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHVB7_000576"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHVB7_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1912665.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHVB7_000577"
     CDS             1788..2183
                     /locus_tag="ACHVB7_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1912666.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHVB7_000578"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHVB7_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR1912667.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHVB7_000579"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHVB7_000579"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR1912668.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHVB7_000580"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHVB7_000580"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR1912669.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHVB7_000581"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHVB7_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR1912670.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHVB7_000582"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHVB7_000582"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR1912671.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHVB7_000583"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHVB7_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR1912672.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHVB7_000584"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHVB7_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR1912673.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHVB7_000585"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHVB7_000585"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR1912674.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHVB7_000586"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHVB7_000586"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR1912675.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHVB7_000587"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHVB7_000587"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR1912676.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHVB7_000588"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHVB7_000588"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR1912677.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHVB7_000589"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHVB7_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR1912678.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHVB7_000590"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHVB7_000590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR1912679.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHVB7_000591"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHVB7_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR1912680.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHVB7_000592"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHVB7_000592"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR1912681.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHVB7_000593"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHVB7_000593"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR1912682.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHVB7_000594"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHVB7_000594"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR1912683.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHVB7_000595"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHVB7_000595"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR1912684.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHVB7_000596"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHVB7_000596"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR1912685.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHVB7_000597"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHVB7_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR1912686.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHVB7_000598"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHVB7_000598"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR1912687.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHVB7_000599"
     CDS             complement(26207..26548)
                     /locus_tag="ACHVB7_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR1912688.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHVB7_000600"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHVB7_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR1912689.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHVB7_000601"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHVB7_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR1912690.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHVB7_000602"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHVB7_000602"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR1912691.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHVB7_000603"
     CDS             33227..34570
                     /locus_tag="ACHVB7_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR1912692.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="ACHVB7_000604"
     CDS             complement(34650..34856)
                     /locus_tag="ACHVB7_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR1912693.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="ACHVB7_000605"
     CDS             35181..35735
                     /locus_tag="ACHVB7_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1912694.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..36736)
                     /gene="ytfI"
                     /locus_tag="ACHVB7_000606"
     CDS             complement(35825..36736)
                     /gene="ytfI"
                     /locus_tag="ACHVB7_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR1912695.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="ACHVB7_000607"
     CDS             complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="ACHVB7_000607"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR1912696.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37878..39821
                     /gene="cpdB"
                     /locus_tag="ACHVB7_000608"
     CDS             37878..39821
                     /gene="cpdB"
                     /locus_tag="ACHVB7_000608"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR1912697.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39939..40319)
                     /locus_tag="ACHVB7_000609"
     CDS             complement(39939..40319)
                     /locus_tag="ACHVB7_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR1912698.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40408..41268
                     /gene="qorB"
                     /locus_tag="ACHVB7_000610"
     CDS             40408..41268
                     /gene="qorB"
                     /locus_tag="ACHVB7_000610"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1912699.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41376..42341
                     /locus_tag="ACHVB7_000611"
     CDS             41376..42341
                     /locus_tag="ACHVB7_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1912700.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42449..43111
                     /gene="ytfE"
                     /locus_tag="ACHVB7_000612"
     CDS             42449..43111
                     /gene="ytfE"
                     /locus_tag="ACHVB7_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR1912701.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="ACHVB7_000613"
                     /gene_synonym="dagA"
     CDS             complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="ACHVB7_000613"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR1912702.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="ACHVB7_000614"
     CDS             complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="ACHVB7_000614"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1912703.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45716..46354
                     /gene="ytfB"
                     /locus_tag="ACHVB7_000615"
     CDS             45716..46354
                     /gene="ytfB"
                     /locus_tag="ACHVB7_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR1912704.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46338..>46684)
                     /locus_tag="ACHVB7_000616"
     CDS             complement(46338..>46684)
                     /locus_tag="ACHVB7_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1912705.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT        11454 a        12415 c        11632 g        11183 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
    36721 tgatcctggg taacatatga caaccttatt ttatttggtt attttatgtc gccattaaat
    36781 atctcattag agaagtaaac tatcaaaaaa cgctcattat taaaatgtaa cttattagca
    36841 attgaatttt tgcactttaa atacaatctc tttttattta taacttttta ggttgggaga
    36901 aatcaggatg gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatagac
    36961 actctgaacc ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc
    37021 cagtcgtgaa cgtgcgctcc ggcagctgca gccacagcat gtccagcggc ggtgtcccaa
    37081 atattcgttg gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat
    37141 ttcagcgaag agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc
    37201 agctccgcat ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc
    37261 tgaatctgct tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac
    37321 attacgttca ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc
    37381 gcaatgttaa cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg
    37441 tctaccagcc agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca
    37501 gaaaggaccg gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg
    37561 gcaatatccg ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc
    37621 gtcccgtcgt agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga
    37681 tctaacattt ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc
    37741 tatcgttaat gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg
    37801 cattcttcac tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg
    37861 ataaaaggat gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg
    37921 ccagtgtgaa tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca
    37981 acatgatgga tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta
    38041 cggcaagcct gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg
    38101 gcgatttgat tcaggggagt ccgctggcag attacatgtc ggcgaaagga ttaaaaacag
    38161 gtgatgttca tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg
    38221 gcaatcatga atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat
    38281 tcccttatgt aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt
    38341 atttaattaa agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg
    38401 gctatattgg cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggga
    38461 aagtgacggt gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga
    38521 aaggtgccga tgtcgttgtc gttctggcac attccgggct gtctgccgat ccgtataaag
    38581 tgatggcgga aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt
    38641 ttggtcatgc tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata
    38701 tcaccaaagg cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc
    38761 ttggggtggt cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga
    38821 aagcggaagc tcggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca
    38881 gcaagctggt agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc
    38941 caatcggtaa atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga
    39001 ccgtgcaagt cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc
    39061 cggatctggc aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca
    39121 aaaatgaccc ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg
    39181 ccgatcttta tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga
    39241 aagagtggct ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac
    39301 cacagtcact catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg
    39361 tgaattatca gattgatgtt acccagcccg cccgttatga cggcgagtgc cagatgatta
    39421 ataccaatgc ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg
    39481 ccatgttcct cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg
    39541 gcgacagcca tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga
    39601 ttgctgatga gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt
    39661 tagcaccgat agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata
    39721 aagccgcagc gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg
    39781 atatcgggtt tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct
    39841 ataaatcatc aatcgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg
    39901 ccctacaagt catacaccgt aggccgcgca tccggcagtt atgccgcacg ttcatcccgc
    39961 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    40021 ctcacctgct cgcccagcgg cgtgaggctg tattcaacat gcggtggcac caccggatac
    40081 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    40141 tcgctcaccc cgccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    40201 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    40261 cactgttccg caaagagatt accctctttc agttgttgcg acaggctaac ctgactcatt
    40321 tcatacttac ctttttgtac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    40381 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    40441 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    40501 gttcgtaatc cggcaaaagc ccaggctcta gcagcacaag gcattaccgt gcgtcaggct
    40561 gactacggcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    40621 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    40681 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    40741 ctcggcctcg ccgatgagca cattgagacg gagaaaatgc tggctgattc tggcatcgtt
    40801 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    40861 ctggaacacg gcgtatttat cggtgcggcg ggcgatggca aaatcgcctc agcaacgcgg
    40921 gcagattatg cggcagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    40981 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    41041 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    41101 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    41161 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ccgcccaacg
    41221 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    41281 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    41341 tgaactttct tctgacagac caatggatgc cagtaatgat tagcggcgtg ctgtacgccc
    41401 tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc gttgatcgta ccggaatacc
    41461 cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg gttaattgcg ctgcccattg
    41521 cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg cgactggctt accgccttga
    41581 tgctcaccat gatgggcaac cttatctatt acttctgcct tgccagtgcc attcaacgta
    41641 ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc ggtggtgatt cccgtttttg
    41701 ccaatctgct ttatagccag cgcgacggca aactcgcatg gggaaaactc gccccggcac
    41761 tggtttgtat tggcatcggc ctggcgtgtg tgaatattgc tgagttaaac cacggactcc
    41821 ccgattttga ctgggcacgt tatacctctg gcatcgtgct ggcgttagtt tccgtggtct
    41881 gctgggcgtg gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc gacaaacatc
    41941 cgatgatgtg ggcaacggcg caggcgctgg tcacactgcc ggtttctctc atcggctatc
    42001 tcgtcgcctg ttactggctg aatatacaaa cgccggactt ctccttacct tttggccccc
    42061 gtccgctggt gtttattagt ctgatggttg cgatagccgt gctttgctca tgggttggcg
    42121 cactctgctg gaacgtcgcc agccagcgat taccgacagt gattctcggg ccgctgattg
    42181 ttttcgaaac gctggcaggt ttgctgtaca cctttttact ccgccagcaa atgccgccgc
    42241 taatgacgct gagcggtatc gcgctgttag tgattggcgt ggtcattgcg gtcagagcaa
    42301 aaccagaaaa acctttaact gaatctgtct cagaaagttg acacgctggc agtgagttaa
    42361 ataagcctct gctacgtaag ggttatagct tttgccttaa agatgcattt aaaatacatc
    42421 ttatattatt aagaatgagg tatcagctat ggcttatcgc gaccaacctt taggtgaact
    42481 ggcgctctct attcctcgcg cttcagctct gtttcgtaaa tatgatatgg attactgctg
    42541 tggcggtaag caaacgctgg cgcgcgcggc ggcacgtaaa gaactggatg ttgaggtcat
    42601 tgaagctgaa ctggcaaagc ttgctgaaca accgattgag aaagactggc gtagcgcccc
    42661 gctggcagaa atcattgacc atatcatcgt gcgctaccac gatcgtcacc gcgagcaact
    42721 gccggagctg atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca aaccgagcgt
    42781 gccaaaaggg ctgacaaaat acctgactat gctgcatgaa gagctatcca gccacatgat
    42841 gaaagaagag cagatcctct tcccgatgat caaacaaggc atgggcagcc aggcaatggg
    42901 accaatcagc gtgatggaaa gcgagcacga tgaagcgggc gaactgctgg aagtgattaa
    42961 acacaccacc aataacgtca caccgccgcc ggaagcctgc accacctgga aagcgatgta
    43021 taacggtatt aatgaactga ttgatgacct gatggatcac atcagcctgg aaaacaatgt
    43081 actgttccca cgcgcgctgg cgggtgagta atctaaaagc tggatggcat tgcgccatcc
    43141 agcatgataa tgcggttatt tccgcagttc agcagcccgc ttcttaccaa taaacaacca
    43201 gcccagcccc agtgcgataa accacagcgg ggtgaccaac agcgcctggc gagtgtcatc
    43261 ttccagtgtc agcaacacga ccacgaacac aaagaacgcc atacataccc agcacatcag
    43321 cttgccgagc ggcatcttgt agatcgactt ctcatgcaga tgaggacgct gtttgcggta
    43381 aaccaggtac gagcaaagga taattgtcca gacgaacata aacagaatcg cggaaacggt
    43441 tgtaatcatc gtgaacgcgc caatcacgct aggattcaca tacaacatca ccacgccgcc
    43501 gagcagacag atacacgaga aggtcaaccc tttcgcgggt actgcgcgct tagaaagttt
    43561 agcgaacgct ttcggtgcca caccttcctg cgccagacca aacagcatac ggctggtaga
    43621 gaagacgccg ctgttagcgg aagacgccgc agaggtcagc accacaaagt tgatcacgct
    43681 ggcagccgca ggcaaaccta ccaacacaaa cagttcaaca aacgggctct tctccgggac
    43741 taccgaactc cacggcgtca cggacataat cacaatcagc gcgaagacgt agaacataat
    43801 gatacggatc ggaatggagt taatcgcgcg tggcagtgat ttctccggat ctttggtttc
    43861 cgcagcggtt gtacctacca gctcaatccc tacgaaagcg aaaaccgcta tctggaatcc
    43921 ggcaaagaag ccacttaaac ctttcgggaa ccagccgcca tcattccaca aatgcgcgaa
    43981 tgacgcttcc acaccagtcg gtgactgaaa gtgcatcgcc accataacca ggccgacgac
    44041 aatcagcgac acaatggcga cgattttgat catcgcaaac cagaactcca tctcaccgaa
    44101 cattttcacg gtggcgagat tgagcgtcag cagcagcact atcaccgcca gcgaggcgac
    44161 ccagtcggag agatcgggga accagaactg agcataagca gtgatcgcca ccacgtctgc
    44221 cataccggtt acaacccagc agaaccagta agtccagccg gtgaaatatc ctgcccacgg
    44281 cccgagtaaa tcggaagcga agtcactaaa agatttgtat tccagattcg aaagcagcaa
    44341 ttcccccatt gcccgcatca cgaaaaagag cataaaaccg atgatcatat aaacgaaaat
    44401 gatcgacggc ccggcaaggc taatcgtttt gccggacccc ataaacaacc ccgtaccaat
    44461 ggcaccgcca atggcaataa gctgaatatg tcggtttgtg agattgcgcc gtagcgactg
    44521 ttcagccgga gcctgatcat cggcaacgac ttttacctga tctaccatgt ttttttcttc
    44581 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    44641 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    44701 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    44761 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    44821 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    44881 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcgcct
    44941 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    45001 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    45061 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    45121 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    45181 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    45241 ttcacacctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    45301 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    45361 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    45421 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    45481 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    45541 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    45601 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    45661 gttagcttat tcataggcta tgattgagga acaagacgcg gagcaggagg aaaccatgcc
    45721 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    45781 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    45841 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgccggtgg tgacgcgtga
    45901 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc tgcgggcgca
    45961 attagtcaca ccacaaaatg atcctgatca ggtggctccg gtcgctccag aacctataca
    46021 ggaaggccag ccagaagaac agccgcaaac tacccaaacg caaccattcc agccagatag
    46081 cggaattgat aatcaatggc gctcttaccg tgtcgaaccc ggtaaaacga tggcgcagct
    46141 gttccgcgac cacggactgc ccgctaccga tgtctatgca atggcgcagg tagaaggcgc
    46201 gggtaagccg ctgagtaatt tgcaaaatgg gcagatggtg aaaatccgcc agaacgccag
    46261 cggcgtggtg acgggcttaa ccattgatac ggggaataat caacaggtcc tgtttacccg
    46321 ccagccggat ggcagtttta ttcgtgcgcg gtagatgaag ggtcaaccaa aagccagcta
    46381 aggattgtct ctttgatgac cttacgtctg gcttccagtg cctggggtga aaccatactg
    46441 atgtgataaa ccaggccaag cgtatgctgg ttgatcaaat aatatccccc aagcgcagca
    46501 atattaatat tcacctgcaa tgggtcgata tctggcttaa aaatattgtg tttcttccct
    46561 tcatccaata aggattccat taactgcaag tgcgcatggt taatttctaa gagacgcagt
    46621 gattttgcat aatgcacacc tttactttga ttttcactat gcacaatttt taaaaaccaa
    46681 gggt
//
LOCUS       DAZPJR010000011        45923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPJR010000011 DAZPJR010000000
VERSION     DAZPJR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.012"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHVB7_000617"
     CDS             complement(<1..716)
                     /locus_tag="ACHVB7_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1912706.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHVB7_000618"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHVB7_000618"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR1912707.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHVB7_000619"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHVB7_000619"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR1912708.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHVB7_000620"
     CDS             complement(3650..4705)
                     /locus_tag="ACHVB7_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1912709.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHVB7_000621"
     CDS             complement(4715..5503)
                     /locus_tag="ACHVB7_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR1912710.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHVB7_000622"
     CDS             complement(5521..6573)
                     /locus_tag="ACHVB7_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR1912711.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7207)
                     /locus_tag="ACHVB7_000623"
                     /pseudo
     CDS             complement(6604..7207)
                     /locus_tag="ACHVB7_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7194..8075)
                     /locus_tag="ACHVB7_000624"
     CDS             complement(7194..8075)
                     /locus_tag="ACHVB7_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1912712.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8096..9406)
                     /locus_tag="ACHVB7_000625"
     CDS             complement(8096..9406)
                     /locus_tag="ACHVB7_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1912713.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIVLPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9441..10951)
                     /locus_tag="ACHVB7_000626"
                     /pseudo
     CDS             complement(9441..10951)
                     /locus_tag="ACHVB7_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="ACHVB7_000627"
     CDS             complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="ACHVB7_000627"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR1912714.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="ACHVB7_000628"
     CDS             complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="ACHVB7_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1912715.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="ACHVB7_000629"
     CDS             complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="ACHVB7_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1912716.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMLDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="ACHVB7_000630"
     CDS             complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="ACHVB7_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1912717.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="ACHVB7_000631"
     CDS             complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="ACHVB7_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1912718.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13254..14231
                     /gene="pspF"
                     /locus_tag="ACHVB7_000632"
     CDS             13254..14231
                     /gene="pspF"
                     /locus_tag="ACHVB7_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1912719.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="ACHVB7_000633"
                     /pseudo
     CDS             complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="ACHVB7_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="ACHVB7_000634"
     CDS             complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="ACHVB7_000634"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR1912720.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="ACHVB7_000635"
                     /pseudo
     CDS             complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="ACHVB7_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="ACHVB7_000636"
     CDS             complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="ACHVB7_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR1912721.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="ACHVB7_000637"
     CDS             complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="ACHVB7_000637"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR1912722.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20134..21495
                     /gene="puuA"
                     /locus_tag="ACHVB7_000638"
     CDS             20134..21495
                     /gene="puuA"
                     /locus_tag="ACHVB7_000638"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR1912723.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21644..21808
                     /locus_tag="ACHVB7_000639"
     CDS             21644..21808
                     /locus_tag="ACHVB7_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912724.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21798..23183
                     /gene="puuP"
                     /locus_tag="ACHVB7_000640"
     CDS             21798..23183
                     /gene="puuP"
                     /locus_tag="ACHVB7_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR1912725.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23317..23562
                     /locus_tag="ACHVB7_000641"
     CDS             23317..23562
                     /locus_tag="ACHVB7_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR1912726.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23875..25518
                     /gene="sapA"
                     /locus_tag="ACHVB7_000642"
     CDS             23875..25518
                     /gene="sapA"
                     /locus_tag="ACHVB7_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR1912727.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25515..26480
                     /gene="sapB"
                     /locus_tag="ACHVB7_000643"
     CDS             25515..26480
                     /gene="sapB"
                     /locus_tag="ACHVB7_000643"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR1912728.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26467..27357
                     /gene="sapC"
                     /locus_tag="ACHVB7_000644"
     CDS             26467..27357
                     /gene="sapC"
                     /locus_tag="ACHVB7_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR1912729.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27357..28349
                     /gene="sapD"
                     /locus_tag="ACHVB7_000645"
     CDS             27357..28349
                     /gene="sapD"
                     /locus_tag="ACHVB7_000645"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR1912730.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28351..29157
                     /gene="sapF"
                     /locus_tag="ACHVB7_000646"
     CDS             28351..29157
                     /gene="sapF"
                     /locus_tag="ACHVB7_000646"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR1912731.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29225..29578
                     /locus_tag="ACHVB7_000647"
     CDS             29225..29578
                     /locus_tag="ACHVB7_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR1912732.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29946..30734
                     /gene="fabI"
                     /locus_tag="ACHVB7_000648"
     CDS             29946..30734
                     /gene="fabI"
                     /locus_tag="ACHVB7_000648"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR1912733.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30879..32005
                     /locus_tag="ACHVB7_000649"
                     /pseudo
     CDS             30879..32005
                     /locus_tag="ACHVB7_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32073..34007
                     /gene="rnb"
                     /locus_tag="ACHVB7_000650"
     CDS             32073..34007
                     /gene="rnb"
                     /locus_tag="ACHVB7_000650"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR1912734.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34085..34188)
                     /locus_tag="ACHVB7_000651"
                     /pseudo
     CDS             complement(34085..34188)
                     /locus_tag="ACHVB7_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34242..36232
                     /gene="pdeR"
                     /locus_tag="ACHVB7_000652"
                     /pseudo
     CDS             34242..36232
                     /gene="pdeR"
                     /locus_tag="ACHVB7_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36214..36342)
                     /gene="ymiD"
                     /locus_tag="ACHVB7_000653"
     CDS             complement(36214..36342)
                     /gene="ymiD"
                     /locus_tag="ACHVB7_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR1912735.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36379..36552
                     /gene="yciZ"
                     /locus_tag="ACHVB7_000654"
     CDS             36379..36552
                     /gene="yciZ"
                     /locus_tag="ACHVB7_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR1912736.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36642..37391
                     /locus_tag="ACHVB7_000655"
     CDS             36642..37391
                     /locus_tag="ACHVB7_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR1912737.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37660..37878
                     /gene="osmB"
                     /locus_tag="ACHVB7_000656"
     CDS             37660..37878
                     /gene="osmB"
                     /locus_tag="ACHVB7_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR1912738.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38004..38330)
                     /gene="yciH"
                     /locus_tag="ACHVB7_000657"
     CDS             complement(38004..38330)
                     /gene="yciH"
                     /locus_tag="ACHVB7_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR1912739.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38330..39067)
                     /gene="pyrF"
                     /locus_tag="ACHVB7_000658"
     CDS             complement(38330..39067)
                     /gene="pyrF"
                     /locus_tag="ACHVB7_000658"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR1912740.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39260..40429)
                     /gene="lapB"
                     /locus_tag="ACHVB7_000659"
     CDS             complement(39260..40429)
                     /gene="lapB"
                     /locus_tag="ACHVB7_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR1912741.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40436..40744)
                     /gene="lapA"
                     /locus_tag="ACHVB7_000660"
     CDS             complement(40436..40744)
                     /gene="lapA"
                     /locus_tag="ACHVB7_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR1912742.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40893..41657)
                     /gene="pgpB"
                     /locus_tag="ACHVB7_000661"
     CDS             complement(40893..41657)
                     /gene="pgpB"
                     /locus_tag="ACHVB7_000661"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR1912743.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41827..42417
                     /gene="ribA"
                     /locus_tag="ACHVB7_000662"
     CDS             41827..42417
                     /gene="ribA"
                     /locus_tag="ACHVB7_000662"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR1912744.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42481..45156)
                     /gene="acnA"
                     /locus_tag="ACHVB7_000663"
     CDS             complement(42481..45156)
                     /gene="acnA"
                     /locus_tag="ACHVB7_000663"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR1912745.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45320..45415)
                     /gene="ymiC"
                     /locus_tag="ACHVB7_000664"
     CDS             complement(45320..45415)
                     /gene="ymiC"
                     /locus_tag="ACHVB7_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR1912746.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45529..45696)
                     /gene="yciX"
                     /locus_tag="ACHVB7_000665"
     CDS             complement(45529..45696)
                     /gene="yciX"
                     /locus_tag="ACHVB7_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR1912747.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45699..45839)
                     /locus_tag="ACHVB7_000666"
     CDS             complement(45699..45839)
                     /locus_tag="ACHVB7_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR1912748.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT        10848 a        12140 c        11560 g        11375 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac aggcagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttacctcttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cacttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc agcccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtcgac gtaatgctcc agcgtccact gctgcggcag cagcgtagga tgcagtgaga
     7081 tcgcctcttt cgcgcccttg aacgaggtca tcagcatcac aaaaaatgga aacagcgtga
     7141 tgatgagaaa cagcgccagc ccgcagtaaa aaccgatgcg actgagtgtg cgtttatttg
     7201 ttgccattga ggttcacccg tttcctggtc agcagaatga cagcgaaaat aatgatgaag
     7261 agcaccaccg aaatcgccgc cgctttccct aagtcattaa aggcaaaggc ggttttgtag
     7321 agatagacgc cgagaatatc gaccttggtg gtcagcagat aaacatcagc aaacatgtag
     7381 aacatccaga tagtgcgcag ggtcaccacc gtcgccagga ccggcataat tgcgggcagc
     7441 gtgacgatgc gaaaccgctg ccaggcatta gcgccatcca tctctgccgc ttcgtacagc
     7501 gatttgtcga tggtctgcaa aatcgccaga aacgagataa aggcatacgg gaagtagcgc
     7561 cagatggcga acagcaccac cagcacaaag ctactgcccg gattatcgaa ccacagcggt
     7621 gcctgctcat agagatgcag aagatcgacg ccgaggtagt tcacaatgcc gtagccgttg
     7681 ttgaacatgt atttccaggc aaacaccagc gaaatggacg gcgttacgta ggagaggatc
     7741 accagcgaac gcgcggtttt gcgcaggcgg aactcgcggt tgaaaaacat cgccactgcc
     7801 agcccgagca cggtgctgcc cgccaccacc agcgcggtat accagaccgt catccacagc
     7861 gagtgccaga agccgggatc ggagaggata cgcacatagt tactcacccc aacaaaggtt
     7921 gactcgatgt tgggattgag cggcagacgt aaaaaactga tttcgatatt cgacaccatt
     7981 ggccaggcca ccagaccacc cagcagtaat aagctgggcg cgagaagcag cagcgcaaag
     8041 ggcatatcgg aacgacctga aaaaagcctg ttcattgcgg attcctgtcc tgggtttagt
     8101 gctgttcgat cagttcatcc agctttttct ggctcgcttg cagcgtgcca gggagatcgg
     8161 ctttacccac ggtgacgtta tgcaccatgg agctcaccac gccagaaccc gtcacatcac
     8221 ccatgcgggt aaagttttta tcccctactg cgccaaagac ctgaatattt ggcagttcac
     8281 cgattagctg attcggtagt tcacccagcg ccttaataac gtcgttgtct ttccaggtgg
     8341 cggtagtgac aaccgcttta ttcaccggca gcgcagcacc tggcgacatc atcacccagt
     8401 cggcaatgtt gtctgcctgc tccataaagg tgacaaattt ttctgctgct tccgtctctt
     8461 cggccttttg cccggcggta atggtcagcg aggtcaacat gccgtagacc gcagagtttt
     8521 tctcggttgg caccacgaaa ccgacgtttt tcgggtcgcc ttctttaatt acagccggaa
     8581 ggatataggt ggagtaaatc gccatcggcg cggtgccgtt cataaaggcg tctttcactt
     8641 ccatgatgtc gttagaaccc ggcatgtcgt tagaacccgg catagtgttg gcagcaaggt
     8701 cgcggtaata ggtcagtgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
     8761 cgttaaagac attagcctgg ttggataacg caaactggga gaaggattgt tccgtcaaca
     8821 cgctttctgc tgtaggcagc acaatgccgt attttttatt cgccgggtca ttcagtttct
     8881 gtgcaacgtc cagtagctgt tgccagtttt tcggctcctc cagccccgct tttgccagca
     8941 catctttgcg ataccagatg ccgccaatcc aggcgctgac gggaacaccg gtccaggcac
     9001 tcccatcttc ggtgcgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt
     9061 tgctgatgac cgtggcaacc gtcttgcgat cgataagctg ctctttatcc ataactttgg
     9121 cgtagtcatg gctggtttcg atcacttccg gcagcgagcc gctacgtgaa agggtgatga
     9181 ctttggtgtt ataagcgtcc tcttccacgg gcacttgttt aacggtgata cctgggtttt
     9241 ctttttcaaa acgggcaatc aatttgctga taactgcctg acgctcctgc tccaccgaag
     9301 aatgcataaa ttcgatggat acatgggttt tattttcttc tttacagcct gaaatcaggg
     9361 cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaga tgttcctttt
     9421 tatttaatcc acataacctg ttaggggcgc agcgttattt cattacccaa tagatcaact
     9481 tcactaatca gatcccgacc atgcagatta gtaatattta catgctgaat atttttactg
     9541 acattaaaca atccagtcag acaatcacca tcagcgttac ttctttgaat acgcattacg
     9601 gatgaattaa tcgtatcgat ggtaaaatta ttatccggat gaaactcgtt atggctgcga
     9661 cgaagtgtaa ttaaacgcga caactcatga tataccgcat gccttaatgt agcttcatcg
     9721 ttcagttctc gggttatctc tttactgtga tattttttac ggttaatcgc acggttatat
     9781 ccgagttttt cgacacctgc gtaatcatta cgcgagccaa gaatactttg aatatatatc
     9841 gctggcacac cggggaaact taacaaaatc gcatgggcga gaataaacct ggcgcaacgt
     9901 tcttcatcgc tactctcacg gcggcttaac gcatccatat gtgagaggcg aggaagttaa
     9961 accaggtggt gttgctggaa ggtagtgtca ggttttgcgc ccacgcacaa agcgcctcaa
    10021 cgttctgttt ttgcaccgca tgcagcacca gtggcggcag cgagaactgg tacaccatat
    10081 gtgcttcgtc atcgcctgcg ccaaagtaag caatgttgtc tttatgcgga acattggtct
    10141 cggtaatgat cactgtacct ggcgcaacgt tatcaataat cgaccgtaac agtttgataa
    10201 tcagatgtgt tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa
    10261 cggcatccag gcggacatac tcagcacctt tcgcaaggta acagagtaaa acatccacca
    10321 tcgccagcaa cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg
    10381 tccataaatg gcgcgttgaa tgatcgcgca tctggaatgg cgttaataac ggtaacgcac
    10441 gcgggcgagt gacggcgctg agatcggttt gcggatcaac cgcaataaaa aaatcttcaa
    10501 aacctggctg ctgttgtaaa tagtttttaa accattcact ttttgccgac atatggttgc
    10561 agacaaaatc aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc
    10621 ccgcttcact ggcgacctga tgataatcaa ttaccgaaaa cccatcatca gatgaacatg
    10681 gataaaaagg taataaatga acatgtgaaa aaatgctttg cagccattga tggtaaaact
    10741 gattaaatgt gggtaatggt tttaaatcat tgctgtgaaa ttgatcggca taggtaatta
    10801 acacgacatc actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac
    10861 tctcaagacg cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt
    10921 cgtccaggta atccgtaatt ttctgtttca tgttgccttt ttgtggtagc ggtcccatga
    10981 aacgagagac tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc
    11041 aggaaatcat tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatgg
    11101 ggatgtgaat aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc
    11161 ttttaacctt tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg
    11221 gtatatccca tctcgctaag gatctctttt gcttgccctg actggcgtcc ggcattgcaa
    11281 tacactttca cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc
    11341 ttcaggggaa tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga
    11401 acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt
    11461 aagccttttt taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc
    11521 ctgagattta acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat
    11581 tagcagcccg acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc
    11641 gagcaactga ttttatcgcc cagcccgcta cacccgccgg gccatagcgc agtgcggtaa
    11701 gaagtaccag cttgcctgct aatttgaaac caggctttac cttttgcccg gcctgttgcc
    11761 agcgagtatt cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc
    11821 ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc
    11881 gacttcatcc agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccag
    11941 cattggatca agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc
    12001 gaagaaaatc gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt
    12061 ggcaatcccg gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt
    12121 attgagatta atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata
    12181 gattccagcg cctgaatacg ttcgcgcatc cgttttgctt catcagccag ttgcgctaat
    12241 cgctgctgct cactttgcga caattcatcg cgaccagaac gattgctgta atgcagccat
    12301 aaccagatcg gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg
    12361 ctcataagta ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt
    12421 attgattgtc ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat
    12481 cggctttcag ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt
    12541 ctgcttccgc ttccatctgg tcaattcgac gttcgaaaga ttcgaaacga gccattgctt
    12601 catccagttt gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat
    12661 gacgtaacat caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc
    12721 caatctcttt cttcatgcgt gccagcgtat cgttcaccag cgtcacttca tgttccaggg
    12781 acttaatcag atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc
    12841 tctctttcag cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt
    12901 gttcaatacg gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta
    12961 cttcaaccag tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt
    13021 ccgctttctc taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa
    13081 tacccataat gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata
    13141 caatttgcgt gccaattttt tatctttttg atttataaag atctgattga agaatcaaca
    13201 gcaacatgcc aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg
    13261 aatacaaaga taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt
    13321 cgcatctcgc accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag
    13381 agctgattgc cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc
    13441 ttaactgcgc ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg
    13501 gggcgtttac cggtgcgcaa aaacgtcatc cagggagatt tgaacgtgcc gacggcggta
    13561 cgctatttct tgatgaactc gctacggcac cgatgatggt gcaggagaaa ttattgcgcg
    13621 tgattgagta cggtgaactg gagcgcgttg gcggcagcca gccattgcag gtgaatgtgc
    13681 ggttggtatg cgcgacgaat gccgatctcc cggcgatggt caatgaaggc acttttcgcg
    13741 ctgacctgct cgaccgactg gcttttgatg ttgtacaact gccaccactg cgcgagcgcg
    13801 aaagcgacat aatgttgatg gcagaacact ttgccatcca gatgtgtcgg gaaatcaagc
    13861 tgcctctgtt cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc
    13921 cgggaaatat tcgtgaattg aaaaacgcgg tggaacgttc agtgtatcgc cacggcacca
    13981 gcgattatcc gcttgatgac atcattattg atccctttaa acggcgtccg cctgaagacg
    14041 ctatcgccgt ttcagaaacc acctcgcttc cgacgctgcc gctggattta cgtgagtttc
    14101 aaatgcagca ggaaaaagag ttgctgcaac tcagtttgca aaaggggaaa tataaccaga
    14161 agcgcgcagc tgaattgctg gggttaacct accatcagtt ccgcgcgttg ttgaaaaaac
    14221 accagattta gcgaagttgg gcaatgcgat tcgccggatg cggcgtgaac gctttatccg
    14281 gcatacataa tcaacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg
    14341 ttgggctatt aatcgctcag cgcatcctgc aaaattttca ttgccgcatc gaattgcgca
    14401 tccgggatgg tcagcggata caggaagcga atcacgttgc cgtatgcgcc acaggtcagc
    14461 aggagcagcc cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc
    14521 tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg accccaggcc gcgtaccgca
    14581 gcaatggccg gaacgctttc tttggcatca atcaacgtgt ttttgagacg ctggcccagt
    14641 tgattcgcgc gttcgcagag tgattctttg ttgataatat tgagcaccgc gtgcgcggca
    14701 gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc cgcccggcgc gggcgcgtcc
    14761 ataatattcg cgttaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc
    14821 atcgtcatta agtccggctt atcggcgtaa tgatccatgg caaacagttt accggtacgc
    14881 gcaaagccgc tttgcacttc atcggcaatc atcacaatac cgtgctcgtc gcacaggcgg
    14941 cgaatggcgg caaccagctc ttttggcgca acgttgaaac cgccctcgcc ctgcaccggt
    15001 tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg atttaaacaa gcgttcgatg
    15061 gcgtcgaggg agtcctatgt tgaaatgccg tgtaaatctg acggataagg tacgtgatac
    15121 accgaaccag ggaacgggcc gaagccgatt ttgtacggcg caacttttcc ggtcagcgcc
    15181 atggtcatat atgtacgacc gtggaagcca ccgctaaacg caatcacgcc agggcgtccg
    15241 gtatgggcgc gagcaatttt caccgcgttt tccaccgctt ccgcaccggt agtgaagaac
    15301 gcggttttgg cctgcccgct caccggggca agggcgttga ttttctccgc cagggtgacg
    15361 tagctttcat acggcacaat ctgatacgcg gtgtgggtaa actgttgcag ttgctgctcc
    15421 accgccgcga ccagatcagg gtggcgatgt ccggtattca gcaccgcaat gcctgcggcg
    15481 aaatcgatgt actcgttgcc ctcaacatcc ttcagagtag cgttttcagc cgactgggcg
    15541 aagaagttac acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat
    15601 tcattgttgc tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc
    15661 cagacgatcg cgcaggctgt accaggctgc gcccatcgcg gtaaacggta ctcgcagcgt
    15721 gcgcccgccg gggaacgggt aatacggcag attggcgaag gcatcgaaac gttcggcgtc
    15781 gccgcgcagc agttcggcaa tcaaacgtcc ggcgagatga gtacaggtga cgccgtggcc
    15841 gctgtaaccc tgcatgtaat agatgttggt atcaaggcga ccaaactgcg gcatgcgcga
    15901 cagggtcagc aggaagttgc ccgtccagcg gtagtcgatt ttgacgccct tcagttgcgg
    15961 gaaggttttc agcagtttcg gcacaacaag acgctcaacg tcatccgggt cgcgcgcgcc
    16021 gtagaccacg ccgccgccgt acagcaaacg gttgtcggcg gtaagacggt agtaatcaag
    16081 cagataatta cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc
    16141 cgacagccgt tcagtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgccagttc
    16201 cggctctact ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac
    16261 ctgacctttg gcagtgcgca ccaccgctgg cgtggtgtgc tggatctgcg tcacggcaga
    16321 aagctcatac acgcgcccgc cgttcaggcg gatggcatcg gcttcaccaa tggcgaggtt
    16381 tagcgggtga atatgcccac cgctgtgatc caacagtgcg ccagtgtagc gatcgctggc
    16441 gacctcgcgg cgaatggcgt tagcgtccag cagctccagc tgtttattgc cgtagcgttc
    16501 ccagttctct ttctgctctt cgagtgtggc gagctgttta tcattcatcg ccacaaacag
    16561 gccgccgggg cggtagtcgc agtcaatctg atagcgtttg atacgttcgc ggatgatctc
    16621 gccaccctcg aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc
    16681 gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc ccgccattgc gcccgcttgc
    16741 gccaaagccg atgcgtgagg cttcgagaac cactacgtca aagcccgctt ccgccagatg
    16801 cagcgcggag gagagcccgg tatagccgcc gccaaccacg caaacgtcgc aggtgattga
    16861 ctcattcagt gtgtcgaatg gtgcatattt attcgcactg gcggcgtagt aactgctggt
    16921 atgttcggtc atttcaggcc tccaggctta tccagatggt tttcagttca gtgaattttt
    16981 caagggcatg cagggatttg tcgcgaccgt tgccgctctg cttatagccg ccaaacggca
    17041 cggtcatatc gccgtcgttg tagttattga cgaagacgga accggctttc aggcggcggc
    17101 tcatacggtg cgcgcgggag aggtcgcgcg tccataccgc cgcgccaagg ccgtactggc
    17161 tgtcgttggc aagctgtagc gcctggtctt ctgatgtgaa acgcgtgacc accagcaccg
    17221 gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc aacatccaca aagatggtcg
    17281 ggccgatggc ggcagccagc tcagcgttac gaccatccag cagcagttgc cctttgcttt
    17341 cgccttcacg aataaagcta tggaccgagt cggcgtgggc gcaatcgatt aaggtgccca
    17401 tggtggttac ggtatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca
    17461 gggctaagaa ttcatcggcg atgctctctt ccagtaacaa acgcgttccg gcgatgcaca
    17521 cctggccctg gttgtagaag atgcctgcgg cggtggcgct tgccgctttt tgcagatccg
    17581 gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca
    17641 tgttgctatc acccgcgtct ttcagcagtt gtttacccgt acgcgtggag ccggtaaagg
    17701 cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc ggcttcatga ccataaccgc
    17761 tcactacgtt cagcacgcca tccggcagcc ccgcttcttc ttttgccagc ccggcgagac
    17821 gaatcgcact gagtggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca
    17881 gcgccgggcc gagtttccag caagtcagca gcagcgggaa gttccacggc acgatggcgg
    17941 caatcacgcc gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca
    18001 cttcgccata cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg
    18061 gaatatcatc acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg
    18121 ccagctcttc ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt
    18181 tagccggaga agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg
    18241 catggtcgat atcgacgctc ttgccgcggg caattttcgc cagcggtgcc taggtgaccg
    18301 gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata
    18361 agcggttttc aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat
    18421 tcattatgac tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca
    18481 gagatccccg ggccgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg
    18541 cattgcgctt acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct
    18601 gataattcgg caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat
    18661 ggcataactt tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc
    18721 accttccagt actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt
    18781 gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac
    18841 cagcttcatt gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac
    18901 ctgcggctca tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac
    18961 cttcagcagc ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact
    19021 gatagcactg tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg
    19081 ctggcggatt tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt
    19141 tccttaaact gtagtggtca gagtcgctgt ttttcagcga tatggtgctg acaagcggtg
    19201 ataaagccct cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc
    19261 cactgtacgc ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc
    19321 ggcgagcgag cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga
    19381 gagtttaccc aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca
    19441 acctgaactt catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga
    19501 tgttccagca attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc
    19561 accagttctt gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca
    19621 ttaattatcg ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt
    19681 tcaccatata ggtgcggctg cacattgctg ggactaccag gaagataaat gccatcgagt
    19741 ttcggcaaaa gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc
    19801 aagccgcctg cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga
    19861 cccttaagcc tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc
    19921 atgctcaatc tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta
    19981 tgttcaatat tttttcaatc tagcagtggg ttttcatttt tgcaaactca atttaacatt
    20041 tgacaaacat ttagtttgca tacagattcg aatggtggtc attatatttt acgctttgat
    20101 aacgagcgga aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag
    20161 aactttgttc agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc
    20221 tacctggagc gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt
    20281 tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat
    20341 ttcccggcct cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg
    20401 ggtcaggaaa tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct
    20461 tctgccgccg atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc
    20521 gctccctttg acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag
    20581 cgcgacgctg aagggaatct gcaaccgccc tgcgcgcctg gcaccgatga ccgcaataca
    20641 caaagccagg tttactccgt tgataacctc aaccacttcg ccgacgtgct caatgatatt
    20701 gatgaactgg cgcagttgca gctgattccg gcagatggcg cggtcgctga ggcctcgccg
    20761 ggtcagtttg aaatcaacct ttaccatact gataacgtgc tggaagcctg cgatgatgcg
    20821 ctggcactaa aacgcctggt gcgtctgatg gcaggaaagc ataagatgca cgccactttt
    20881 atggcgaagc cgtatgaaga gcacgcgggc agcgggatgc atatccatat cagtatgcaa
    20941 aataatcgtg gcgagaatgt gctttctgac acagaaggcg aagattcgcc gctgctgaaa
    21001 aagatgctcg ccgggatgat tgacctgatg ccgtcgtcga tggcgttgct ggcaccaaac
    21061 gtgaactcgt atcgccgctt ccagccggga atgtatgtgc cgacgcaggc gtcgtggggc
    21121 cataacaacc gcaccgtcgc cctgcgtatt ccgtgcggcg accgtcataa tcaccgcgtg
    21181 gaatatcgcg tggcgggtgc cgatgccaac ccatatctgg tgatggcagc gatttttgcc
    21241 ggtattttgc atggccttga taacgagctg ccgttgcagg aagaagtcga aggcaacggg
    21301 ctggaacagg aaggcttacc cttcccgatt cgccagagcg atgccctggg tgagtttatc
    21361 gagaatgatc acctgcgccg ctatttaggc aaacggtttt gccatgtgta tcacgcctgt
    21421 aaaaatgatg agctgttgca gtttgagcgc ctcatcacag aaacagaaat tgagtggatg
    21481 ttgaaaaacg cgtaattccc cttgcggggt tgtttcatcg gcctgagcgg agccaggcct
    21541 gacatttgct ggtaatccag acatttcccg gatgtcgcgt tatggagcgc aggcggcaac
    21601 gggcttccgt taacgattaa gactgtgacg aggaaatgag atgatgccga tgattaaatc
    21661 cccgcatggc gagggaggct gcgtatgcgc ccctcctgct actgactgga ctccgccccc
    21721 tttgcttccc ttgctaaaca ggtttgattt caggtcgaca cgaccgcaaa ccttattacg
    21781 caggggaggc agcaattatg gctattaatt caccactgaa tattgctgcg caacccggca
    21841 aaacccgtct gcgaaaatca ctgaaattgt ggcaggtggt aatgatgggt ctggcctatc
    21901 tcacgccgat gaccgtattt gatacttttg gtattgtctc cggcattagc gacggtcacg
    21961 ttccggcgtc ctatttgctg gcgctggcgg gcgtgctgtt taccgctatc agctacggca
    22021 aactggttcg ccagtttccg gaggccggtt cggcctatac ctacgcgcaa aagtcgatta
    22081 acccgcacgt cggatttatg gtcggctggt catcactgct ggattatctc tttttgccaa
    22141 tgatcaacgt tttgttggcg aaaatctatc tctccgccct cttcccggaa gtgccgccgt
    22201 gggtgtgggt ggtaaccttc gtcgccattt taaccgccgc gaatctgaag agcgtcaacc
    22261 tggtcgctaa cttcaatacc ctgtttgtac tggtgcaaat ctccatcatg gtggtgttta
    22321 tcttcctggt ggttcaggga ctgcataaag gagaaggcgt tggcaccgtc tggtcacttc
    22381 agccgtttat cagcgagaac gcgcacctga tcccgattat taccggtgcg acgattgtct
    22441 gtttctcgtt cctcggtttc gatgcggtga ccacgctttc ggaagagacg ccggacgccg
    22501 cacgcgtgat cccgaaagcc atcttcctga cggcggtata cggcggcgtt atctttatcg
    22561 cggcgtcgtt ctttatgcag ctgttctttc ccgatatcag ccgctttaaa gacccggacg
    22621 ccgcactgcc tgaaattgcg ctgtacgtcg gcggcaagct ctttcagtcg attttcctct
    22681 gcaccacgtt tgtgaacaca ttagcgtctg gcctggcttc acatgccagc gtgtcgcgtc
    22741 tgctgtatgt gatggggcgt gacaatgtgt ttccggagcg cgtgtttggc tatgtgcacc
    22801 caaaatggcg gactccggca ctcaacgtca ttatggtcgg gattgtcgcg ctgtcggcgc
    22861 tgttctttga tttagtcacc gcgacagcac tgattaactt cggtgctctg gtggcgttta
    22921 ccttcgtgaa tctgtcggtg tttaatcatt tctggcggcg taaaggaatg aataaaagct
    22981 ggaaggatca cttccactat ttgctgatgc cgctggttgg cgcgctgacg gtgggtgtgc
    23041 tgtgggttaa cctagagtca acgtcactga cactcggtct ggtatgggct tcgttaggcg
    23101 gcgcatattt gtggtatctg atccgccgct atcgcaaagt gccgctgtac gaaggtgaca
    23161 gaacgccggt gagcgaaacg taatagccgg atgcgcggcg cattatcctg cctgagggta
    23221 acgcgccgcg ccattaacta taaaaagatg tataacccgg atcacgagtg ctatcatccg
    23281 cccttatgct tagcgcatta accccagggg caaataatga accacgatat cccgctaaaa
    23341 tattttgata ttgccgatga gtacgcgacc gaatgcgcag agcctgtcgc agaggctgaa
    23401 cgcacaccgc tggcccacta cttccagtta ctgctcaccc gtttgatgaa caatgaagag
    23461 atcagcgaag aagcccaaca tgaaatggct gctgaagcgg ggattaatcc cgtacgcatt
    23521 gatgagatcg cagagttttt gaatcaatgg ggtaatgagt aattatttaa ttgcagacga
    23581 tcggtcagta tcgttgtccg gataaagcgt tcacgccgca tccggctaac aatgacacgc
    23641 actgcagcgt gctttaatgg gtttctcacg atcgtaccgc tctgctcctc gctaagttgt
    23701 tctgtgagtt atagttatag agaaatcacg ggtacgaatg ttgaacagat ggcgacgcca
    23761 cctgttcaac gaccaaccac acggccctca ttacgacccc tgaacacaaa cggtaatggc
    23821 aaatgtcatt aaatattagt aagatagcct ctttacataa aatcccttaa tattatgcgc
    23881 caggtattat cgtctctttt ggtgattgct ggacttgtga gtggtcaggc aatcgccgcg
    23941 cctgaatccc ccccgcatgc tgatatccgc gacagcggtt ttgtctattg cgtcagcggg
    24001 caagtcaaca cctttaaccc atccaaagcg agcagtgggt taattgtcga tacccttgcc
    24061 gcccagtttt atgatcgact gctggatgtc gatccctata cctatcgcct gatgccggaa
    24121 cttgccgaaa gctgggaagt actcgacaac ggcgcgacct atcgcttcca cctgcgtcgc
    24181 gatgttccgt ttcaaaaaac cgactggttt actcccactc gtaaaatgaa tgccgacgat
    24241 gtggtgttta ccttccagcg aatttttgac cgcaacaacc cgtggcataa cgtcaacggc
    24301 agcaacttcc cctacttcga cagcctgcaa tttgccgata acgtgaaaag cgtccgcaaa
    24361 ctggataatc ataccgttga gttccgactc gcccagccgg atgcttcttt tttgtggcac
    24421 ctcgcaaccc attatgcttc ggtcatgtcg gcagaatatg cccggaagtt agagaaagaa
    24481 gatcgccagg agcaactcga ccgtcaaccg gtcggcaccg gtccgtatca gttgtcggaa
    24541 taccgcgccg ggcaatttat tcgcctacaa cgtcatgatg acttctggcg cggtaaaccg
    24601 ttaatgccgc aggtagtggt ggatttaggc tccggcggca ccggacgtct gtcgaaactc
    24661 ctgaccgggg aatgcgacgt tctggcctgg cctgctgcca gccagctatc cattttgcgt
    24721 gacgacccgc gcttgcgttt aacgctgcgt cctgggatga acgtcgccta tctggcattt
    24781 aacaccgcca aaccgccgct aaataatccc gctgtccgcc atgcactggc actggcgatt
    24841 aataaccagc gcctgatgca atccatctat tatggtacgg ctgaaacggc ggcctctatt
    24901 ttaccgcgcg cctcgtgggc ctatgacaac gaggctaaaa ttactgaata caatccggcg
    24961 aaatcgcgcg aacagttgaa ggcgttgggg ctggaaaact taacgctgaa actgtgggtg
    25021 cccacacgtt cgcaggcgtg gaaccccagt ccactgaaaa ctgccgaact gattcaggcg
    25081 gatatggcgc aggttggcgt aaaagtggtg attgtgccgg tagaaggtcg ctttcaggag
    25141 gcgcggttga tggatatgag ccatgatctg acattatccg gttgggcgac ggacagtaac
    25201 gacccggaca gtttcttccg tcctttactg agctgcgcgg caattcattc acagaccaac
    25261 ctcgcccact ggtgcgatcc gaaattcgac agcgtgttgc gtaaggcgct ctcctcgcag
    25321 cagctggcgg cgcgtattga agcctatgac gaagcgcaga gtattctggc gcaggaatta
    25381 cctattctgc cgctggcgtc gtcattgcgt ttgcaggcct atcggtacga tatcaaaggt
    25441 ctggtactta gcccgtttgg taacgcctcc tttgctgggg tgtatcgcga gaaacaggat
    25501 gaggtgaaaa aaccatgatt atcttcacct tacgccgcat tttgttattg attgtcacct
    25561 tgttcctgct gaccttcgtt ggcttcagct tgagctattt cacccctcat gcgccgttac
    25621 aaggcgcgtc actgtggaat gcctgggtgt tctggtttaa cggcctgatc cactgggatt
    25681 ttggtgtttc cagcattaac ggtcaaccga ttgccgaaca gttaaaagag gtcttcccgg
    25741 cgacgatgga actgtgcatc ctcgccttcg gttttgcgct gattgttggc atccccgtgg
    25801 ggatgattgc cgggattacg cgccataagt ggcaggataa cttgatcaac gccatcgcct
    25861 tactgggctt ttcaatcccg gtgttctggc tggcgcttct gttgacgctg ttttgttcac
    25921 tcacgctagg ttggttgccc gtttcagggc gtttcgatct gctctacgaa gtgaaaccga
    25981 ttaccggttt tgcgttgatt gatgcctggc tttcggactc accatggcgg gatgaaatga
    26041 tcatgagcgc aatccgccat atgatattgc ccgtgatcac cctgtcggtt gcgccaacaa
    26101 ctgaagtgat ccgcctgatg cgtatcagca ctatcgaagt gtatgaccag aactatgtaa
    26161 aagcggcggc gacccgcggt ttgtcgcgct ttactatttt gcgtcgccac gttctgcata
    26221 acgcgttacc gccggttatt cctcgcctgg gcttacagtt ttccaccatg ctgacgctgg
    26281 cgatgatcac cgaaatggtt tttagctggc cgggtttagg gcgctggtta attaacgcca
    26341 tccgccagca ggattacgca gccatttccg ccggagtgat ggtgtgtggc tcactggtca
    26401 ttattgttaa cgtgatttct gatattttgg gtgccatggc taaccctctg aaacataagg
    26461 aatggtatgc cttacgatag cgtatacagc gaaaagcgcc cgccgggcac gctgcgtacc
    26521 gcctggcgca aattttatag tgacgcctct gcgatggtcg gcctgtacgg ctgcgcgggg
    26581 ctggctgtac tgtgtatttt tggcggctgg tttgcgcctt acggcatcga ccagcaattt
    26641 ctcggttatc aattattgcc gccatcatgg tcgcgctatg gcgaagtttc tttcttcctg
    26701 gggactgacg acctcggacg cgatgtttta agccgtttac tgagcggagc tgcgccaacc
    26761 gtgggtggcg catttgtggt gacgcttgcc gcgacgatct gtggcctggt actgggcacc
    26821 ttcgccgggg cgacacacgg cctccgctca gcggtgctta accatattct ggatacctta
    26881 ctggcgatcc cttcgctgct gttggcaatt atcgttgtag cgtttgccgg accgagtttg
    26941 tcccacgcta tgtttgctgt ctggctggca ttgctgccgc gtatggtacg ttcgatttac
    27001 agcatggtgc atgacgaact ggaaaaagag tacgttatcg ccgcccgtct ggatggcgca
    27061 tcaacgctga atattctctg gtttgctgtg atgccaaaca tcaccgccgg gctggtcacc
    27121 gagatcaccc gcgcactgtc gatggcaatt ctcgatatcg ccgcgctggg ctttctcgat
    27181 ctcggcgcac aactcccctc gcctgaatgg ggagcgatgc tcggtgatgc gctggaactg
    27241 atttatgtcg ccccgtggac tgtcatgctg ccaggtgcgg caattatgat tagcgtgttg
    27301 ttggttaacc tccttggcga tggcgttcgt cgtgcgatta ttgcgggggt ggaataatgc
    27361 cattactcga tattcgtaac ctgaccattg aatttaaaac cggtgatgag tgggttaaag
    27421 ccgtcgaccg ggtaagtatg accttaaccg aaggtgaaat ccgcggtctt gttggtgaat
    27481 ccggttccgg caagagtttg attgcgaaag cgatttgtgg ggtgaataaa gataactggc
    27541 gtgttactgc tgaccgtatg cgttttgatg atatcgattt gctgcgtctc tccgcacgcg
    27601 aacggcgcaa actggttggc cataacgtgt cgatgatttt ccaggaaccg cagtcgtgtc
    27661 ttgacccttc agaacgtgtg ggtcgccagt tgatgcaaaa catcccagcc tggacctata
    27721 aaggccgttg gtggcagcgt tttggctggc gcaaacgccg tgccattgaa ctgctgcacc
    27781 gcgtggggat taaagatcac aaagatgcga tgcgcagttt tccctatgag ttgaccgaag
    27841 gtgaatgtca gaaagtgatg atagccattg cactggcgaa tcaaccgcgt ctgctgattg
    27901 ctgacgaacc gaccaactca atggagccaa caacccaggc gcaaatcttt cgcctgctga
    27961 cgcgtctcaa ccaaaacagc aataccacta ttttgcttat cagccatgac ttacaaatgc
    28021 ttagccaatg ggcggataaa attaacgtgc tttactgcgg tcaaacagtg gaaaccgcgc
    28081 cgagtaagga gttggtgacg atgccacatc atccttatac ccaggcgctg atccgcgcga
    28141 taccagactt cggcagcgcg atgccgcata aaagtcgcct caatacgctg cccggcgcta
    28201 tcccactgct ggaacagtta ccgattggtt gtcgtctggg gccacgttgc ccgtatgcac
    28261 aacgagaatg cattgtgacg ccacgtttga cgggggcgaa aaatcatctc tatgcctgtc
    28321 atttcccgct gaacatggag aaagagtgag atgatcgaaa cgctgcttga agtgcgtaat
    28381 ttaagtaaaa cgttccgcta ccggaccggc tggtttcgtc gtcagaccgt agaagcggta
    28441 aaacccttga gctttacgct acgtgaaggc cagacactgg cgattattgg cgagaatggt
    28501 tcgggtaaat ccacgctggc aaaaatgctg gcgggaatga tagagcctac cagcggcgag
    28561 ttattgattg acgatcatcc actgcatttt ggcgattatt ccttccgcag tcagcgtatt
    28621 cgcatgattt ttcaggatcc ttcgacctcg ttgaatcccc gtcaacgtat ctcgcaaatt
    28681 ctggattttc cactgcgcct gaacaccgac ctggaaccgg aacagcgtcg caaacagatt
    28741 attgaaacga tgcgtatggt tgggctgcta ccggatcacg tcagttacta tccgcatatg
    28801 ttggcacccg gacaaaaaca gcgtctgggt ctggcgcgcg cgctaatatt gcgcccaaaa
    28861 gtcattattg ccgatgaagc cctcgcttca ctggatatgt cgatgcgttc gcagttgatt
    28921 aatctgatgc tggagttaca ggaaaaacag ggcatttcgt atatttatgt tacccagcat
    28981 atcggaatga tgaagcacat tagcgaccag gtgctggtga tgcatcaggg cgaggttgtc
    29041 gagcgaggca gcaccgcgga tgtgctggca tcgccgctgc atgaactcac caaacggctg
    29101 attgccgggc actttggcga agcattgaca gcggatgcgt ggcgtaaaga tcgctaattt
    29161 tctgaatcct ccttccagat tatccacctc attctgacaa gagattaaat cttgtcagaa
    29221 tgaggtgatg gataaaataa aatcaaatgc gcgtgattta agacgcaatc tcactttgca
    29281 ggaacgaaag ctctggcgat atcttcgcag ccgacgtttt ggtgatttca aatttcgccg
    29341 tcaacatccg gtggggagct acattctcga ttttgcttgc tgctcggcgc gcgtagtcgt
    29401 tgagctggat ggtgggcagc atgatttggc agttgcctat gataccaggc gcactagctg
    29461 gcttgagtcg cagggctgga ccgtgctgcg tttctggaat aacgagattg attgtaatga
    29521 ggaggcggtg ccggagatta ttctgcagga actgaaccgc cgatcaccct caccctgaaa
    29581 gggcgagggg gccgaccgag ccgaatagct gttgtggtga aaacatggag acagtgctgg
    29641 agaatattcg gcaaggtctg aaccatccca gccatcgcca taaaagggtt aggggctgta
    29701 tgagcctgtt tgttgctggg gtaacaatat ttgcacaata tggtcccctc gcccctctgg
    29761 ggagagggtt agggtaaggg gaaaagcgcc caccctgccg cagcctgctc cggtcggacc
    29821 tggcaactat agctactcac agccaggttg attataataa ccgtttatct gttcgtactg
    29881 tttactaaaa cgacgaatcg cctgattttc aggcacaaca agcatcaaca ataaggatta
    29941 aagctatggg ttttctttcc ggtaagcgca ttctggtaac cggtgttgcc agcaaactat
    30001 ccatcgccta cggtatcgct caggcgatgc accgcgaagg agctgaactg gcattcacct
    30061 accagaacga caaactgaaa ggccgcgtag aagaatttgc cgctcaattg ggttctgaca
    30121 tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga caccatgttc gctgaactgg
    30181 ggaaagtttg gccgaaattt gacggtttcg tacactctat tggttttgca cctggcgatc
    30241 agctggatgg tgactatgtt aacgccgtta cccgtgaagg cttcaaaatt gcccacgaca
    30301 tcagctccta cagcttcgtt gcaatggcaa aagcttgccg ctccatgctg aatccgggtt
    30361 ctgccctgct gaccctttcc taccttggcg ctgagcgcgc tatcccgaac tacaacgtta
    30421 tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc
    30481 cggaaggtgt gcgtgttaac gccatctctg ctggtccgat ccgtactctg gcggcttccg
    30541 gtatcaaaga cttccgcaaa atgctggctc attgcgaagc cgttaccccg attcgccgta
    30601 ccgttactat tgaagatgtg ggtaactccg cggcattcct gtgctccgat ctctctgccg
    30661 gtatctccgg tgaagtggtc cacgttgacg gcggtttcag catcgctgca atgaacgaac
    30721 tcgaattgaa ataatcattc tgttggtaaa tattgggcgg cgttctgccg cccgttatct
    30781 ctgttatacc tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc
    30841 gtcaggataa cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg
    30901 gcaaaagcca ctggtatcat gaaacgcaat ccagtactgc ggagtatgac gttctgcctc
    30961 tggtcccgga agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag
    31021 aaacgctggc tcccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggaattgt
    31081 tccctgacca gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcta
    31141 cggccctgac ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc
    31201 gacttacgcc gctccccggg cctgattcct ccagggaaag caatcatagg ttggcacaaa
    31261 tcacgcaata tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc
    31321 agcatctgaa tgacgtcggt cttactgcct gggactgtgt gatcattaac caaatcattg
    31381 gttttattgg ctttcaggcg cggaccattg cgacatttca ggcttatctc gggcacccgg
    31441 tacgctggtt acccggtctg gaaatacaaa actacgccga cgcgtcactg tttgctgatg
    31501 aatcaatacg ctggcgaagc agctatgagg tggaaaaact acctgaagag tacacaaaaa
    31561 gttcaacagc agaactttgc caactggccg aaacactctc tctccaccct atttcacttt
    31621 cccttctcga aaggttgtta aacagtacac gggttaatac acagccggat aataagcttg
    31681 cggcgttgtt atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatggatt
    31741 catcaaatga atataaaaaa tcagccccct tctgcgcaag ggcgaaaatg aaattaacca
    31801 atgggctgac cgtcattctg ttgagcacgc taccgttcag gcgatacaat ggctgacccg
    31861 agcacccgat cgctttagcg ccgcccagtt cagcccttta ctcggacacg aaaaatcatc
    31921 aacgcagatt attaatctgc tggtatggag cgggctgtgt ggctggataa atcgcttaaa
    31981 aatcgcgttg ggtgagacat attaaccttg ccgcgtcaga cagattcgcg taaaactgtc
    32041 agccgctcta atggccacca aaatagacaa ttatgtttca ggacaacccg ctgctagcgc
    32101 agcttaaaca gcaactgcat tcccagacgc cacgcgctga aggggtggta aaagccacag
    32161 aaaaaggctt tggcttcctg gaagtcgacg cgaaaaaaag ttatttcatt ccgccgccgc
    32221 agatgaaaaa agtcatgcat ggcgaccgaa ttatcgcggt gatccacagt gaaaaagaac
    32281 gtgaatccgc agagccagaa gaactggttg aaccgttcct gactcgtttc gtgggtaaag
    32341 ttcagggcaa aaatgaccgt ctggccatcg ttcctgatca tccactctta aaagacgcca
    32401 ttccttgccg cgcagcccgt ggcctgaacc acgagtttaa agaaggcgac tgggcggttg
    32461 ccgaaatgcg ccgtcatccg ctgaaaggcg atcgttcttt ctatgctgaa ctgacacaat
    32521 acatcacttt tggtgacgac cactttgtac cgtggtgggt tacccttgcg cgccataatc
    32581 tggaaaaaga agcaccagac ggcgtcgcta ccgaaatgct cgatgaaggt ctggttcgtg
    32641 aagatctgac cgcgctggat tttgtcacca tcgacagtgc cagcacagaa gatatggatg
    32701 acgccctttt cgctaaggcg ttgccggatg acaaacttca gctgattgtg gcgattgccg
    32761 atccaaccgc gtggattgct gaaggcagca agctggacaa agccgcgaaa attcgcgcat
    32821 tcaccaacta tctgcctggc ttcaacatcc ctatgcttcc tcgcgagctt tctgacgatc
    32881 tctgctcact gcgcgccaat gaagtccgcc cggtactggc atgccgcatg acgctctccg
    32941 ctgatggcac cattgaagat aatatcgaat tctttgccgc caccatcgaa tccaaagcga
    33001 agctggtgta tgaccaggtt tctgactggc tggaaaatac cggtgactgg cagcctgaaa
    33061 gtgaagcaat tgccgaacaa gtccgtttgc tagcgcaaat ttgccaacgc cgcggcgagt
    33121 ggcgtcataa ccacgcactg gtgtttaaag atcgcccgga ttaccgcttt attctcggtg
    33181 aaaaaggtga agtgctggat atcgtcgccg agcctcgtcg cattgccaac cgtatcgtcg
    33241 aagaagcgat gattgccgct aacatttgtg cggcccgcgt tctgcgcgat aagctcggtt
    33301 ttggcatcta taacgtgcat atgggctttg atccggcgaa tgccgacgcg ctggcagcgt
    33361 tgctgaaaac gcacggtctg catgtcgatg ccgaagaagt gctcacgctg gacggtttct
    33421 gcaaactgcg tcgtgaactg gatgcgcaac caactggttt cctcgacagc cgcattcgtc
    33481 gcttccagtc atttgctgaa attagcactg aacccggtcc tcactttggc ctcggtctgg
    33541 aagcatacgc cacctggact tcgccgatcc gtaaatatgg cgacatgatc aaccaccgtc
    33601 tgctgaaagc ggttatcaaa ggcgaaactg cgacgcgtcc gcaggatgaa atcactgtcc
    33661 aaatggccga acgtcgccgt ctcaaccgga tggcagaacg tgatgttggc gactggttat
    33721 acgcacgctt cctgaaagat aaagccggga ccggcacccg tttcgcggcg gaaattgtcg
    33781 atatcagccg tggcggcatg cgtgtgcgtt tggttgataa cggcgctatc gcctttattc
    33841 cggcaccttt cttacacgct gtgcgcgatg aactggtttg cagccaggaa aacggcactg
    33901 tacaaattaa aggtgaaacg gtttacaaag taactgacgt tattgacgtc accattgccg
    33961 aagtccgcat ggaaacccgc ggcattattg cgcgcccggt cgcgtaatct cctttcacgg
    34021 cccattcctc atggatgggc cgtttatttc cccgctctac cttcgttata tccaggcaga
    34081 ttttttaact gactttcgtt tgaaaactgg cgtttttcca aaactggatt acaaataaat
    34141 atataaaaaa acaatgcgtt tagccgccgt taatttcatg taagtcatgt tgttcctttt
    34201 atttaaccgc ggactccgca gttaaccgga ggatatgcat catgaaaacc gttagggagt
    34261 ccacaacgtt gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca
    34321 gcgatgtttt gcgctttttt accaccgaaa ccacagaacc tgatcgtact ttgcagttat
    34381 ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga
    34441 gtctgaccgt cgatgagagc gatctttctg tgcatctggt aggacgaaaa atcaataaac
    34501 gggaatgggc tggcaacgcg tctgcatggc atgacacacc ggcagttgct cgtgatttat
    34561 cacacgggct ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc
    34621 tcgacagccg ggggaatatc caacgcttca atcggttatg tgaagattac actgggttga
    34681 aagaacacga cgtcattggc caaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg
    34741 cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac
    34801 tgtggatacc aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa ataaatttgt
    34861 ccacagcggc agtggcaaaa acgagatttt tttaatctgt tccggtaccg acattaccga
    34921 agagcgccgc gctcaggagc gactgcgtat tctggcaaat accgacagta tcaccggact
    34981 gccgaatcgt aacgcaatgc aggagttaat cgatcacgct attaatcagg ctgataacaa
    35041 taaagttggg gttgtgtatc ttgatttgga taattttaaa aaggtcaacg acgcctatgg
    35101 gcatttgttt ggtgaccagt tattacgcga cgtgtcattg gctattttaa gctgtctcga
    35161 acatgaccag gtgttggcgc gtccaggtgg cgatgagttt ctggtactgg catccaatac
    35221 ctcacaaagc gcgctggaag caatggcatc acgaattttg acccgcttac gcctcccctt
    35281 tcgtattggt ttaattgaag tttataccag ctgttcagta ggtatttcgc tctctcccga
    35341 acatggttcg gacagcgcga ctattattcg tcacgccgac acagcaatgt acacagcgaa
    35401 ggaaggcgga cgaggacaat tttgcgtttt tactccagaa atgaatcagc gggtatttga
    35461 atatctctgg ctggatacca acttgcgtaa agcactggaa aacgatcagt tggttattca
    35521 ctatcaaccg aaaatcacct ggcgtggcga agtgcgcagt ctggaagcac tagtacgttg
    35581 gcagtcacct gaacgtgggt tgattccacc gttggacttc atttcctacg ccgaagagtc
    35641 agggctaatt gtgcctttag gccgttgggt gattctcgat gtcgtacgcc aggtggcaaa
    35701 gtggcgggat aaaggcataa acctgcgagt ggcggtaaat atttctgcac gtcagctcgc
    35761 cgatcaaacc attttcaccg ccctgaaaca ggttctccag gaactcaatt ttgaatactg
    35821 ccctatagat gttgaactga cagagagttg tctgattgag aatgatgaac tggcactgtc
    35881 tgttattcaa caatttagcc aactaggtgc gcaagtgcat ctggatgatt ttggtaccgg
    35941 ctactcttca ctttcgcaac tggcgcgctt tccgatcgat gccatcaaac ttgaccaggt
    36001 ttttgttcga gatattcaca aacaacctgt ctcgcagtca ctggtccggg cgatcgtcgc
    36061 tgtggcccag gcattgaatc ttcaggtgat cgccgaaggt gtagagagtg caaaggaaga
    36121 tgctttttta accaagaacg ggatcaatga gcggcaagga tttttgtttg ccaaaccgat
    36181 gcccgccgtc gccttcgaac gctggtataa acgctatctg aagcgcgcat aagtagatga
    36241 atatatatac ttagcgctat atcccgtcat tatatatcgt attcctggat gataattagc
    36301 cgttgctgtt aaacacttct cttttagcgg caatttttgc attattcatt ttccactttt
    36361 gctataagga tgtccttgat gtctgaattt gatgcccagc gcgttgccga acgtattgat
    36421 attgtgttag atatccttgt ggcaggcgat taccactctg cgatccataa tctcgagata
    36481 cttaaagcag aactcttgcg ccaggttgct gaatctacgc cagacatccc gaaggcaccg
    36541 tgggagattt aacaatcgtg ccttcaggtt ttcctatttg aggatgcccg gcgcgtcagt
    36601 atttttttat ttagtattat aacgttataa gaattacagc gatgaattcc cgacaacaaa
    36661 ctattctaca gatggtcatt gaccagggtc aggttagcgt aaccgatctg gcaaaagcca
    36721 ctggagtttc tgaagttacc attcgccagg atctcaacac cctcgaaaaa ctgagttacc
    36781 tccgccgtgc acatggcttt gcagtttcgc ttgatagtga tgacgtcgaa acccgtatga
    36841 tgagcaacta tacgctgaag cgtgaactcg ccgagtttgc cgcgtcactg gttcaaccgg
    36901 gcgaaaccat ctttatcgaa aatggcagca gcaatgccct gctcgcccgg actctggggg
    36961 agcagaagaa aaatgtcact atcatcacgg tcagcagcta catcgcgcat ttgctgaaag
    37021 acgcgccttg tgaagtcatt ttgctcggtg gcgtgtacca gaaaaaaagc gaaagtatgg
    37081 ttggcccgtt gacacgccag tgcatccaac aggtgcattt cagcaaagca tttattggta
    37141 ttgatggctg gcaacctgaa actggattca ccggtcgcga tatgatgcgt accgatgtgg
    37201 tcaatgccgt gctggaaaaa gagtgcgaag cgatagtcct gactgacagc tcgaaatttg
    37261 gtgctgtaca ttcatactcc atcggtcccg ttgagcgatt caatcgcgtg attaccgatt
    37321 cgaaaatacg cgccagcgat ctgatgcatc ttgagcacag taaactcacc gttcacgtcg
    37381 ttgacattta accatttccc ttctctacgg atgatttgca gtttggcaaa tcatccgctc
    37441 taagatgatt cctggttgat aattaagact atttacctgt tattaacacg cttaagatat
    37501 aaaattatta tcagcgatat aacaggaagt cattatcacc tgcgtgatat aaccctgcgc
    37561 gcgagcagat ttcacggaat aatttcacca ggcttattct tagctattat agttatagag
    37621 agcttacttc cgtgaatcat aaattcagga gagagtatta tgtttgtaac gagcaaaaaa
    37681 atgaccgcgg ctgttctggc aattactttg gcaatgtctc tgagtgcctg ttctaactgg
    37741 tctaaacggg accgcaacac cgcaatcggc gcgggtgcag gggcattagg cggtgcagta
    37801 ctgaccgatg gcagtacgtt gggtacatta ggtggtgcag ctgtcggtgg tgttattggt
    37861 caccaggtcg gtaaataatc gtaaataaat gtcgattatc gactcgcaat tataatacgc
    37921 ttgattctgt tcagtacgaa ataactgaat aatacgcaca gtaaaatatt cgaaagccac
    37981 ggatatatcc gtggcttttt atgttaaccg cctgcgagtt ttaccttcat ccctttcgct
    38041 tcaagtagtg attttaataa atcacgctta tcgccctgga tttcaataac tccatcttta
    38101 actgctccgc cgcagccgca ttttttcttc agttccgctg caagttttgt cagttcggca
    38161 tcatcgagat cgacaccggt aatcaggcaa acgcccttac ctttacgtcc actggtctga
    38221 cgctgaatac gcaccacacc gtcgccttta gggcgtacag gggccgcttt gggctcatca
    38281 atacgtccgg tttccgttga gtagaccaga cggctgttgg aatcactcat catgcactcc
    38341 gctgtaaaga ggcgttgatc gctttcagcg tctgcgctgg atctaccgat tgcgttaccg
    38401 ggcgaccaat caccatataa tcaacaccag ccgacaacgc ctgttctggc gtcataatgc
    38461 ggcgctggtc accagcttca ctcccctgcg gacgaatgcc cggcgtaacc agtttgaact
    38521 cctgaccgaa tacctgttta aagcgcacag cttcctgagc agaacacacc acaccatcaa
    38581 ggccacattt ttgcgtcagt gccgccagac gttctgcata atctgcaggt gacagtgtca
    38641 tgccaagatc gaccaggtcg ctggcttcca tgctggtcaa cactgtcaca gcaatcaaaa
    38701 gcggtgcatc tttgccaaac ggaaccagtg cctcacgcgc tgcggtcatc atacgcgccc
    38761 caccagaggc atgaacattc accatccaca cgcctaagtc agctgcagca gcgacagcgt
    38821 gcgctgcagt gttaggaata tcgtggaatt tcaggtcaag aaagatatca aaaccacgct
    38881 gttgaagttc gcgcacaaac tgtggcccaa acaatgtaaa catctctttg ccgaccttca
    38941 gacgacaatc gcgtgggtcg atcttgtcga caaaggacag cgcgtcatca cgattatgat
    39001 aatcaagggc aacaaccaca ggagaattcg taacagcgcg ggaagaagat gaagcagtta
    39061 acgtcatgac cagaccttct tgatgatggg caccggaagt ggcgcgaaac aggtaaacgg
    39121 cgagcattct acccgtggaa ataaaaaatt aacagttgcg atttttcccc tggcaggctt
    39181 tttctgctcc gtcgttgccg gagcacaatg gaacatcaat aactagtatg ttgtaactaa
    39241 agtcggattt ttttaaaaat tacaggccat caagaccgcg aatcggttta atggttgacc
    39301 aggcccgaca agacggacaa tgccagtaga gggtgtatgc ggtaaaacca catttctggc
    39361 agcgataacg aggcttacta cgtacctttt cgccaaccat gtcacgcagc accatcaggc
    39421 tctctttggc acgcccttct tccgcttcat ttaagtggta atccattaac ttatggaaca
    39481 cacgcatggt cggatgacgc tgaagctggc gcgtaatata gacctgtgcg gcctcactac
    39541 cgtcgcgcgc ttcgatgata tcagcaagca tcaattcagc atcggcaccg gtgttctctt
    39601 ccaccgcgcg ttgcaggaat tctgcccatt cggcagtttt acccaactgc tgatagcagg
    39661 tttgcaacat ttccagcgtt tcgctgacca gttctctgtc ctgggatatc acgcgttgca
    39721 aactttcgac ggctttggcg tattctcctt tcgccataaa cacgcgtccc atcatgatgg
    39781 atacgcgggc gctgttttta tctgccgccg cccctttttt tagcaaggtc atggcacgat
    39841 cgagatcgtc gctggccata tgctgcaggg ctaactcaca gtagaaatgg gcaatttcga
    39901 cgcgctgttt atctttaccc agcttcacca ggcgttcggc aacatcaatt gctttctgcc
    39961 actcgctggt agcctggtag atttgtagca actgttgcag cgcgccaatg cggaagtcag
    40021 tttcatcggt cagctgattg aacatgtctt ccgcgcggtc gtataacccg gcggccatgt
    40081 aatcacgccc cagttgttga atcgccaaca gacgctgttc ataggtcagt gaggcgcttt
    40141 ccattagggt ctgatggatg cgaatagcgc gatcaacttc gccacgcgaa cggaacaggt
    40201 ttccgagcgt aaggtgggct tcaacggtgc ctgtatcctc tttaagcata tcgagaaaca
    40261 ggtctaccgc tttatcctgt tgattactaa gcaggaagtt aacccccgct acgtaatcac
    40321 gcgacaagcg gttggcttca tcttgcttgt tttgttgcgc acttctgcgg cccatatacc
    40381 agccataggc agcggctaca ggcaaaagca gaaacaacaa ctccagcata gaaagttatt
    40441 ccttcgccgc tgacgagtgc ggcactacag ccacgtcagt cgcgggtgaa agctggtttt
    40501 ccagtcgctt tattttacgt tcagcgcgcg ccagggaaac acgaactcgc agccagaaca
    40561 ggccacaaat caaccaaccg atagcaaccc ccgcagcaaa caataccgcc agcaatgtgg
    40621 aaatacggta ctccccttgc gctaacagat aattaaacgt cacctgttga tcgttctgcg
    40681 cacccaacgt gaccgaaatc acgaagatcg ctaacaccag taagaaaatg agtaaatatt
    40741 tcacattact tcccgttatg tggttaaagc gaataaatta tggtcaactt accgcaatag
    40801 ccctttaaag taccattttc gcgccgtctg cgaaatggca tccacgcgca ttgttgatga
    40861 tttaagggct aaattcggaa aatcaaccag cgttaacttt cttgttctcg ttgcgctatt
    40921 tcgcgatttt cttccgcagg tggtgttaat ggcccacaaa ttcgttgcgc aagccacgtt
    40981 gccaccgcca ccagcgccca cgaaatcaac gtagctacta ccagatcgcg tggccaatgc
    41041 atcccgagca gcaggcggct tcccatgact cccgttgccc agaccagcaa gatagcaatg
    41101 gttaacgttc gccgacgcgg ccacagcaaa ccaacggcca gcagtgccca actggcagca
    41161 aacatcgtgt gaccggaagg aaaggcaaac cccgtctctt tctgccagtg tgaacgcaaa
    41221 tattgtggga tatttttctc ttcagccaac tgttctttca ctagatttcc gcgttctgct
    41281 cgctttaaag tgtagaactc atcaaccgga atatgatgtg ttttttccag ccagataaca
    41341 aaaggtcgtg gttcctggac tttgtctttg atccaggatt taacgccttg tcccacaatg
    41401 attacggccg ccagaatggc aaataataca aaggcagcct taatgcaaaa acgcagacac
    41461 cagagaaacc agccgaataa aatcaaatgt gtaatgacgc cccagggctg ggtgacagtt
    41521 tcagtaaccc aaaaagccgc ttttagtagc caactttgtt ctccaggttg ccaacgccag
    41581 ccagaaatcc atacggctac tggcatgaca agcaatagtg cagctcccac tgcggtacgt
    41641 ctggcaatcg aacgcatggc ctctcctttt tgataagtcc cacaatcata actgaaaacg
    41701 ccagttccag gaaaaattga cagatttgtg ccattccgtg aacgatcgac gcgtcgtgat
    41761 taggtgaacc ccttctcgtt atggcaaaat aagccaatac agaaccagca ttatctggag
    41821 aatttcatgc agcttaaacg tgtggcagaa gccaaactgc caaccccatg gggcgatttc
    41881 ctgatggtgg gatttgaaga actggcaacc ggacacgatc atgtcgcgct agtctatggc
    41941 gatatttccg ggcatacccc ggtacttgcg cgcgtccatt ccgaatgtct gaccggtgac
    42001 gccctgttca gcttgcgctg cgattgtggc ttccagctcg aagcggcatt gacgcaaatt
    42061 gccgaggaag gccgtggtat tttgctgtat caccgtcagg aaggtcgtaa cattggtctg
    42121 ctgaataaaa tccgcgctta cgcactgcag gatcaaggtt acgataccgt agaggctaac
    42181 catcagttag gcttcgccgc tgatgagcgc gacttcactc tttgcgctga tatgttcaaa
    42241 ctccttggcg tcaatgaagt ccgcttgtta accaataacc cgaaaaaagt cgaaattctg
    42301 accgaagcag ggattaatat tgttgaacgc gtaccattga ttgtaggtcg taaccccaat
    42361 aacgaacatt atctcgatac caaagccgag aaaatgggtc atttgctgaa caaataaccc
    42421 tctcgcattg tggaattaat ttgcttgccg gaagcaaaat aaccggcaag caaatatttg
    42481 ttacttcagc atattacgaa tgacataatg caaaatgccg tcgttctggt agtaggtcaa
    42541 ctccgtcgcg gtgtcgatac gacaacggca gggtacgacc tcctggctac catccgcgcg
    42601 cgtaagcgtc accggaaccg tcgcaccggg ttgtagattt tgcagatcgc caatatcaat
    42661 cttctcttcc ccggttagtc ctaacgtttt acgcgttacg ccttgcggaa attccagcgg
    42721 caggatgccc atgccaatta aattcgaacg gtgaattcgt tcaaacgatt cggcaatcac
    42781 cacacgaatt ccaagcagac gaggaccttt tgccgcccag tcacggcttg agcctgaacc
    42841 atactctttc ccggcaatca ccgccagcgg cgtttgttcc tgcttatagc gcatcgcagc
    42901 atcataaata gagactacgt cgctgtcagg taaatgccgc gtcatccccc cttcaacgcc
    42961 aggcaccatt tcattacgga tgcgaatatt ggcgaaggtg ccgcgcatca tcacttcatg
    43021 gttaccacgc cgcgaaccgt aggagttaaa gtcttttcgc tcaacacccc gaccttgtag
    43081 atatcgaccc gctgggctgt cgggcttaat actgcccgcc ggagagatat ggtcagtggt
    43141 gactgaatcc cccagcattg cgaggatccg cgcaccgtga atatcttcca ctggtgctgg
    43201 tgttgcctgc atttcatcaa agaaaggcga taagcgaata taggttgagt cctcctgcca
    43261 accgtaggta tcggatcgtg tgacgttaat ttccttccac tctgctgtgc cttcaaaaac
    43321 ttctgcgtac tctttgcgga acatttctgt ggagacttgt tctaccgcac gggcaatttc
    43381 ttgtgccgat ggccagatat ctttcagata aaccggatcg cctttacgat catgaccaat
    43441 aggctcagaa gccaggttga tattcatatt tcccgccagc gcataggcaa ccaccagcgg
    43501 cggcgaggcc agccagttgg ttttaaccag cggatggata cggccttcaa agttacggtt
    43561 gccggacagc actgcaccga cggttaaatc gccttttttg attgccgttt cgataggatc
    43621 gggcagcggc ccagagttac caatacaggt agtacaaccg tatcccacaa ggttaaaccc
    43681 cagttcgtcg agaaacggtg tcagtttcgc ttttgccaga tagtcagaaa caactttcga
    43741 gcctggtgcc agcgacgctt tgacccacgg ttgccgcttg aggccaagaa ttacggcttt
    43801 tttcgccagc aagcctgcag ccatcagcac acttgggtta gaggtgttgg tgcacgaggt
    43861 tatcgcagca atgaccacag cgccatcagg taactgatac tgatgtccgt tcataacata
    43921 atcgaccggc tggcgatctt tatgcgtggc attcacttcc agttcgttac tggcggcaaa
    43981 tacttttggt acatcgggca gtgcaacgcg atcctgtggg cgttttggcc ctgccaggct
    44041 cgcttcaacg tcattcatat ccagttctaa cgtactggta aaaatcggtt catcgcccgg
    44101 gttacgccac atgccctgcg ctttggcata tttttcgacc aactcaacct gatcttcgct
    44161 gcgcccgctt aaacgcatgt aatcgagggt tacagcatcg attgggaaga agccacaggt
    44221 ggcaccatat tctggcgaca tattggcaat ggtggcgcga tccgccagcg gtagtgaatc
    44281 cagaccatca ccataaaatt cgacgaattt ctccaccacg ccatgtttgc gcagcatttg
    44341 ggtaacagtg agaaccaggt ctgtggcggt aataccttca cgtaatttac cggtaagttt
    44401 aaagcccact acgtccggga taagcatgga aaccggctgg cctaacattg cggcttctgc
    44461 ttcgatccca cccacgcccc accccagcac gccaaggccg ttgatcatag tggtgtgcga
    44521 gtcagtacca acgagtgtat ccggataagc aatccattca ccgtcctgca attcactcca
    44581 cactgctttg ccgagatatt cgaggttaac ctgatggcaa atgcctgtgc ctggcggcac
    44641 gacgctaaac cgactgaacg cttgctttcc ccatttcagg aacacataac gttcgtggtt
    44701 gcgctccatt tccaggcgta cgttttcttc aaatgcctca tcatcaccaa aacgatcgac
    44761 ggtcaccgag tggtcaatga ccaggtcgac cggtgagagc gggttaacct ttgcagtatc
    44821 gccgccgagg cgtttaaccg cttcgcgcat tgccgccaga tcaacaacgg caggtacgcc
    44881 ggtaaagtcc tgcatcaaca cccttgccgg gcggtaggca atttcacggt cagcatgggc
    44941 atttttcagc catcctgcca gcgcgtggat atcctcttcg gtaaccgagt taccatcctg
    45001 ccagcgcagc aggttttcga gcaaaacttt gagtgacttg ggtagacggg tgatatcgcc
    45061 cagtgattta gcagcaagcg gcaggctgta gtagtggtaa gttttatctt tggcctgcaa
    45121 cgtgtcctta ctggcttctc gtagggttga cgacatagct cctccttaat gacagggttg
    45181 cgaagccctg attctcttca gggtgaatat taaagataac acaaacatcg cgtaacgatt
    45241 tgataacaac ccaaattgat aaaagaggag aaaccttgtg gcaaagcaaa tgacaacccc
    45301 gccgcagcgg ggtcaaggat cagtgagtaa tgatccagag gagttcggcc cagaagagca
    45361 tcgacagaga aaacgcgccc atccaggacc aatatttcat atttgtgttg atcataggct
    45421 acacccttaa cggttattat tttgtgttga gataactcaa gctgttattg ctgcggtgta
    45481 ttaaaagccc tttaattcat taaaaatggg taataagaat gatgataatt attgtttcgg
    45541 ctgatcatct tcagcaaaca gttctataaa ggcttgctgc tgcgatgtta gttgccagtc
    45601 gtctggaata acgacggaac attcccgttt cgtttctgtt tcattgtcac tatgctggca
    45661 taatggtgac gactccagtt gctcctgacc gaccatagtt atccccacac tttccaaatc
    45721 agcagtgcga caaccaccca gaaaagtgca gaaccaagaa aaaccgccag ccaggcttta
    45781 cgttttaatt caggatcgcg gcggggttcc tgatttccag aaggcattgc taacctcata
    45841 taatcgccat cacttatcag caagacgttg aacgattggt gtaattaatc atacgatgag
    45901 acaaatccta aagaaacttt agt
//
LOCUS       DAZPJR010000012        45605 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPJR010000012 DAZPJR010000000
VERSION     DAZPJR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45605
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.013"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(4..1863)
                     /gene="gss"
                     /locus_tag="ACHVB7_000667"
     CDS             complement(4..1863)
                     /gene="gss"
                     /locus_tag="ACHVB7_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR1912749.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2068..2934
                     /gene="yghU"
                     /locus_tag="ACHVB7_000668"
     CDS             2068..2934
                     /gene="yghU"
                     /locus_tag="ACHVB7_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR1912750.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2963..3044)
                     /locus_tag="ACHVB7_000669"
                     /pseudo
     CDS             complement(2963..3044)
                     /locus_tag="ACHVB7_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3057..3305)
                     /gene="hybG"
                     /locus_tag="ACHVB7_000670"
     CDS             complement(3057..3305)
                     /gene="hybG"
                     /locus_tag="ACHVB7_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR1912751.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3318..3659)
                     /gene="hypA"
                     /locus_tag="ACHVB7_000671"
     CDS             complement(3318..3659)
                     /gene="hypA"
                     /locus_tag="ACHVB7_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1912752.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3652..4140)
                     /gene="hybE"
                     /locus_tag="ACHVB7_000672"
     CDS             complement(3652..4140)
                     /gene="hybE"
                     /locus_tag="ACHVB7_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR1912753.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4133..4627)
                     /locus_tag="ACHVB7_000673"
     CDS             complement(4133..4627)
                     /locus_tag="ACHVB7_000673"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR1912754.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4627..6330)
                     /gene="hybC"
                     /locus_tag="ACHVB7_000674"
     CDS             complement(4627..6330)
                     /gene="hybC"
                     /locus_tag="ACHVB7_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR1912755.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6327..7505)
                     /gene="hybB"
                     /locus_tag="ACHVB7_000675"
     CDS             complement(6327..7505)
                     /gene="hybB"
                     /locus_tag="ACHVB7_000675"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR1912756.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7495..8481)
                     /gene="hybA"
                     /locus_tag="ACHVB7_000676"
     CDS             complement(7495..8481)
                     /gene="hybA"
                     /locus_tag="ACHVB7_000676"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR1912757.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8484..9602)
                     /gene="hybO"
                     /locus_tag="ACHVB7_000677"
     CDS             complement(8484..9602)
                     /gene="hybO"
                     /locus_tag="ACHVB7_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR1912758.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9791..10078)
                     /gene="yghW"
                     /locus_tag="ACHVB7_000678"
     CDS             complement(9791..10078)
                     /gene="yghW"
                     /locus_tag="ACHVB7_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR1912759.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10197..11084)
                     /gene="yghX"
                     /locus_tag="ACHVB7_000679"
     CDS             complement(10197..11084)
                     /gene="yghX"
                     /locus_tag="ACHVB7_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR1912760.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11241..12281
                     /gene="gpr"
                     /locus_tag="ACHVB7_000680"
     CDS             11241..12281
                     /gene="gpr"
                     /locus_tag="ACHVB7_000680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR1912761.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12321..12815)
                     /locus_tag="ACHVB7_000681"
     CDS             complement(12321..12815)
                     /locus_tag="ACHVB7_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR1912762.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13006..13890
                     /gene="yghA"
                     /locus_tag="ACHVB7_000682"
     CDS             13006..13890
                     /gene="yghA"
                     /locus_tag="ACHVB7_000682"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1912763.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14050..14475)
                     /gene="exbD"
                     /locus_tag="ACHVB7_000683"
     CDS             complement(14050..14475)
                     /gene="exbD"
                     /locus_tag="ACHVB7_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR1912764.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14482..15216)
                     /gene="exbB"
                     /locus_tag="ACHVB7_000684"
     CDS             complement(14482..15216)
                     /gene="exbB"
                     /locus_tag="ACHVB7_000684"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1912765.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15468..16655
                     /gene="metC"
                     /locus_tag="ACHVB7_000685"
     CDS             15468..16655
                     /gene="metC"
                     /locus_tag="ACHVB7_000685"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR1912766.1"
                     /translation="MTDKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16795..17454
                     /gene="yghB"
                     /locus_tag="ACHVB7_000686"
     CDS             16795..17454
                     /gene="yghB"
                     /locus_tag="ACHVB7_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR1912767.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17494..18393)
                     /gene="yqhC"
                     /locus_tag="ACHVB7_000687"
     CDS             complement(17494..18393)
                     /gene="yqhC"
                     /locus_tag="ACHVB7_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR1912768.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18587..19750
                     /gene="yqhD"
                     /locus_tag="ACHVB7_000688"
     CDS             18587..19750
                     /gene="yqhD"
                     /locus_tag="ACHVB7_000688"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR1912769.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19855..20682
                     /gene="dkgA"
                     /locus_tag="ACHVB7_000689"
     CDS             19855..20682
                     /gene="dkgA"
                     /locus_tag="ACHVB7_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR1912770.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20882..21808
                     /locus_tag="ACHVB7_000690"
     CDS             20882..21808
                     /locus_tag="ACHVB7_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR1912771.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21859..22116
                     /gene="yqhH"
                     /locus_tag="ACHVB7_000691"
     CDS             21859..22116
                     /gene="yqhH"
                     /locus_tag="ACHVB7_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR1912772.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22159..24378)
                     /locus_tag="ACHVB7_000692"
     CDS             complement(22159..24378)
                     /locus_tag="ACHVB7_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR1912773.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24489..25901)
                     /gene="ftsP"
                     /locus_tag="ACHVB7_000693"
     CDS             complement(24489..25901)
                     /gene="ftsP"
                     /locus_tag="ACHVB7_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR1912774.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25976..26713)
                     /gene="plsC"
                     /locus_tag="ACHVB7_000694"
                     /gene_synonym="parF"
     CDS             complement(25976..26713)
                     /gene="plsC"
                     /locus_tag="ACHVB7_000694"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR1912775.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26946..29204)
                     /gene="parC"
                     /locus_tag="ACHVB7_000695"
     CDS             complement(26946..29204)
                     /gene="parC"
                     /locus_tag="ACHVB7_000695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR1912776.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(29750..30232)
                     /locus_tag="ACHVB7_000696"
     CDS             complement(29750..30232)
                     /locus_tag="ACHVB7_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR1912777.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30285..30677)
                     /gene="ygiW"
                     /locus_tag="ACHVB7_000697"
     CDS             complement(30285..30677)
                     /gene="ygiW"
                     /locus_tag="ACHVB7_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR1912778.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30829..31488
                     /gene="qseB"
                     /locus_tag="ACHVB7_000698"
     CDS             30829..31488
                     /gene="qseB"
                     /locus_tag="ACHVB7_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR1912779.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31485..32834
                     /gene="qseC"
                     /locus_tag="ACHVB7_000699"
     CDS             31485..32834
                     /gene="qseC"
                     /locus_tag="ACHVB7_000699"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR1912780.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            32944..33525
                     /gene="mdaB"
                     /locus_tag="ACHVB7_000700"
     CDS             32944..33525
                     /gene="mdaB"
                     /locus_tag="ACHVB7_000700"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR1912781.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33556..33870
                     /locus_tag="ACHVB7_000701"
     CDS             33556..33870
                     /locus_tag="ACHVB7_000701"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR1912782.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(33918..35810)
                     /gene="parE"
                     /locus_tag="ACHVB7_000702"
     CDS             complement(33918..35810)
                     /gene="parE"
                     /locus_tag="ACHVB7_000702"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR1912783.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(35839..36420)
                     /gene="yqiA"
                     /locus_tag="ACHVB7_000703"
     CDS             complement(35839..36420)
                     /gene="yqiA"
                     /locus_tag="ACHVB7_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR1912784.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(36420..37247)
                     /gene="cpdA"
                     /locus_tag="ACHVB7_000704"
     CDS             complement(36420..37247)
                     /gene="cpdA"
                     /locus_tag="ACHVB7_000704"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR1912785.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(37272..37694)
                     /locus_tag="ACHVB7_000705"
     CDS             complement(37272..37694)
                     /locus_tag="ACHVB7_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR1912786.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(37695..38324)
                     /gene="nudF"
                     /locus_tag="ACHVB7_000706"
     CDS             complement(37695..38324)
                     /gene="nudF"
                     /locus_tag="ACHVB7_000706"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR1912787.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            38529..40010
                     /gene="tolC"
                     /locus_tag="ACHVB7_000707"
     CDS             38529..40010
                     /gene="tolC"
                     /locus_tag="ACHVB7_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR1912788.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40158..40829
                     /locus_tag="ACHVB7_000708"
     CDS             40158..40829
                     /locus_tag="ACHVB7_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR1912789.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            40835..41995
                     /locus_tag="ACHVB7_000709"
     CDS             40835..41995
                     /locus_tag="ACHVB7_000709"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1912790.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42033..42821)
                     /gene="ygiD"
                     /locus_tag="ACHVB7_000710"
     CDS             complement(42033..42821)
                     /gene="ygiD"
                     /locus_tag="ACHVB7_000710"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR1912791.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            42964..43737
                     /gene="zupT"
                     /locus_tag="ACHVB7_000711"
     CDS             42964..43737
                     /gene="zupT"
                     /locus_tag="ACHVB7_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR1912792.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(43795..43965)
                     /gene="yqiD"
                     /locus_tag="ACHVB7_000712"
     CDS             complement(43795..43965)
                     /gene="yqiD"
                     /locus_tag="ACHVB7_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR1912793.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44226..44879)
                     /gene="ribB"
                     /locus_tag="ACHVB7_000713"
     CDS             complement(44226..44879)
                     /gene="ribB"
                     /locus_tag="ACHVB7_000713"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR1912794.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(44983..45131)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45253..45543
                     /gene="ubiK"
                     /locus_tag="ACHVB7_000714"
     CDS             45253..45543
                     /gene="ubiK"
                     /locus_tag="ACHVB7_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR1912795.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11255 a        12236 c        11454 g        10660 t
ORIGIN      
        1 gtattacttt ttcaccacaa ttaacggttc aatatcactc tcttttttga tgaccagtga
       61 ttcatcaccg cgcaaacacg tcccaccgta gttgccgccc acggtgaagg tacatacctg
      121 aatgtattta ccgtccactt tcggcaaaca ccacagttgc tgatagatgt ttttctgctc
      181 ggcaaattta ccgctggttt tgtccagcac ctcttcatga tggctgacca ggtcgatatt
      241 gctaccacag cgaccggcaa tcggtttcac tgcgtaacct gttttcacca attcatcatt
      301 aacagtgaaa tcggtatcca gcaggtaacg atggtgcggg aacagcgacc agaggatcgg
      361 cagaatcgct ttgttgccgg ggatcaccgt ccacagcggc tcaaagacta gcacttccgg
      421 gcgcagcaat acgtcgataa gacgcacttc gttttgcgga tgaccggtac ggattggcac
      481 cgcagcaaac tcacggtcgc taacttcacg aatctgatca aacgcggttt cccacgccca
      541 ggttttccac acgcagttaa ccagtcgccc ttccccatca atcagttgcc cggcagcatc
      601 ccagcccagt tcatccaatc cacgcaagat acgcgtttca aagcccgcct ggtgcagcgc
      661 ctgctccata aactgcgcgt gatagttttc ctcgatatct ttgtcctgca tgatatggac
      721 aaacggacgt gcacgactgt gtttccaggc accagccaat tcgttaatca gcccttccgc
      781 cggattgaag ccgttgcctt tatagccctg ctccgcccaa cgttcgagga tcaagcccgc
      841 ttcggtatga caggaggcgg agtcggcgtt gtactcgtaa accttcaggc cacgctcatc
      901 catgcagaaa tccatacgac cagtgatcat atggtgacgg cgacgctgcc aggagagacg
      961 caaacgtggc cagaggattt tcgggatgtc gaacagcgcc agcaggttgt catctttcag
     1021 caccttgtcg gttgcgtgaa gatacatcag gtgcagctcg ttggtggctt taattagctc
     1081 ctgctcggca ctctcggtaa tggtgtagta atgataagga tcctgattga tcacctgacc
     1141 gttggcctgc acataggcgt tttgcagcgg atctttttca tccagccatt taccgtcaaa
     1201 ctggcctttg ttttccaggc gcgctccgct gattttcagc agctcgcctg caatttccgg
     1261 ctgcggtaag ctgtattcag tatcttccgt ctggatcatc cagcccagaa tggtggtgtc
     1321 atcaaaagtg tctttcaggg tatagcagcc gttttcgacc accatctcca gctcacgcgt
     1381 ccactgttgc ccttgcggca acggggaatg aatcacgttc tgttccgcaa tacggacttt
     1441 gttgccatgc aattgggtaa tgatggcgac atggccagtg tctttaaatt caccgccttt
     1501 atcccagata agaagcgcac ccgcgaccgg cgcacgcggc gagccgttag gaaatgcctg
     1561 caatggcagg atgttgtcat taaccacttc tcgcaggaag cgcagcgaga aaatctccca
     1621 cgccataccc acgtcagtaa agaccacacc gtaattcaga aagagaaaac ggcgagcaaa
     1681 ttcaacgcat tgccacttgt ggcccatata ttcgtcgtcg atatagctac ggaatacggc
     1741 gtcatcttcg tattcctgcg gatcgagaga actgtaatct gaagagtaga ttgctacccc
     1801 acctggggcg tagcccaata atgtcccgaa cggggcatcc tggctggtcg ttcctttgct
     1861 catcacttta cctttaacaa tacaacctaa gcagttggcg taattgttgt gctctgatta
     1921 cctgctcagc gacattaacc ggacagaggt cagactaatc atacacctca tcgcagcggc
     1981 tggcgcaaag ggttaaaaaa ttcacattct gtacagcaag tgacctgcta cactgcttca
     2041 acactaccac tcagaaggca actcactatg acagacaata cttatcagcc cgcgaaagtc
     2101 tggacgtggg ataaatccgc tggcggcgcg ttcgccaata tcaatcgccc ggtttctggt
     2161 ccgacgcatg aaaaaacgct gcccgttggc aaacacccat tgcaacttta ttcgctggga
     2221 acgccgaacg gtcagaaagt aacgattatg cttgaggagc tgctggcgct gggcgttact
     2281 ggtgcagagt acgacgcctg gctgattcgt attggcgatg gcgatcaatt ctccagcggc
     2341 tttgtcgaag tgaacccaaa ctcgaagatc ccggcgctgc gcgatcatac gcataatccg
     2401 ccgatccgcg tgtttgaatc tggttcgatc ctgctttatc tggcggagaa atttggctac
     2461 ttcctgccgc aggatttggc aaagcgtact gaaacgatga actggctgtt ctggttacag
     2521 ggcgcggcac cgttcctcgg cggtggtttt ggtcactttt accattacgc gccgatgaaa
     2581 attgagtacg ccatcaaccg ctttaccatg gaagccaaac gtctgctcga cgtgctggat
     2641 aagcaactgg cgcagcataa gtttgttgcg ggcgatgagt acaccattgc ggatatggcg
     2701 atttggccgt ggtttggcaa tgtggtgtta ggcggcgtgt atgatgccgc agagtttctt
     2761 gatgcgggca gttataagca tgtacaacgc tgggcgaaag aagtaggcga acgtccggcg
     2821 gtgaaacgtg ggcgtattgt taaccgcacc aacggaccgc tgaatgagca gttgcatgag
     2881 cgccatgacg ccagtgattt cgagacgaat acggaagata agcgtcaggg gtaagggttg
     2941 gtgttcgtcg cagcaagcca tccaggccgg ataaggcgtt cacgccgcat ccggcaatcg
     3001 tgcacaatgc ctgatgcgac gctgccgcgt cttatcaggc ctacgaaagc taatcatcac
     3061 gcgctggtaa tgtcgtaatc catttggcgc agtgcgtcta atgtggcttt ggcttcatct
     3121 tcgtcgatga tgctcatggc aaatccgacg tgtaccagca cccactggcc cagtagatcg
     3181 gcagggttac cttcacaaat cagggcgata ttcacgtcgc gcttgatacc acatacttca
     3241 acctgcgcaa gctggtgaat atcttcaccg acagccagca cctggcctgg aacgccaata
     3301 cacattatta actccggtta ttcaacttca atacttttga cgatcagcga atcgccggta
     3361 tcgacacgca accgctcgcc gtgacagagc ggacactgcg catcgtgctg atgaatctcc
     3421 accacctggc tgcaatccca gcaccaagcc tgggcgggtt tatagacgat atgtaaatcg
     3481 cacccttgcg ccaccgttcc gtggcagaca atttcaaaac taaaacggac ggcgctctcc
     3541 tcaacgcagg agagcgcgcc aatttccagc cacacggcgg tgacgcgctt aacatcgtgc
     3601 tgctccgcct gccgttggat aatttcaacg gcgctctggc aaagagacaa ctcatgcatt
     3661 ttcgccactc ctgcgaccaa acagcagggc gcgacgccct gcgtgtggta catccggatt
     3721 cgtgactggc agcgaaagga tcattcgtgc gcagtcatcc gtcaggcgtt ggccctcttc
     3781 aatcgacatg ctgtgcgaaa gcggcgacat cagcgaacag gagagatatt gcgaaacacc
     3841 gtccagttcg ccaacggtaa aggtcatagt gccatacggc agttgcagac caattttttc
     3901 actcactttg cgcagcggcc agagttgatc cgggccgggg aagataactg cactcagcat
     3961 ccacggggtg atcacacacc ccgtccactg accttcgaac agcgtaaaat cagaaacata
     4021 caccggcatt gaaggatgca gaaaagagag atcgtgcatc gaacgccggg caatttcttc
     4081 aaacgctgcc tgtacttgcg ccttcgggga ggtctggaaa cctgctatct cttcagtcat
     4141 gaatcgcctc ccgtgggatg gcttccacgc ctgattcgcg cagcgcagcc agaacctgct
     4201 caagcgcagg ttcaatcatt gcttcaaccg tcggcgttaa gccgatgtgt ggctccagcg
     4261 attccgggat cacgccgacc agggtcagct ttttcggaaa ctcaccggtg aagcgcaggg
     4321 ccgacaatac gtcggccagg ccaagctgat gcggagagat tttgttggta aacaacgccg
     4381 gaacttcttc atcccgcagg atcatcatcg ttcccggcgt gttctttttc gacacaatgg
     4441 catccgcaat aatcagatga tcgcgatttg ccatgtcgcc aagcagctcc attcccgccg
     4501 tgccgccatc gaggatctca acataatccg gcagaatgta tcgttgctct aacgcttcga
     4561 caatccgcac accgatggct tcatcggtca gcaaaatatt gccgacccct aagactaaaa
     4621 tacgcattac agaaccttca ctgaaaccac ttcgttgccg tcggcatcca ctacgtgtac
     4681 cgcacaggcc atgcacgggt cgaaggagtg aatggtacgc accacttcca gcggtttatt
     4741 cggatcagca accggtgtac ccaccagcga ctgctcgtaa ggaccgacgt catcattgaa
     4801 gttacgcgga ccagagttcc aggttgatgg aacaactgcc tggtagttac tgataatacc
     4861 gtctttaata accatccagt gagagagcat accgcgcggc gcttcgagga agccaacacc
     4921 tttgaactca cccgttgccg gaatgttcgg tttcacaaag gtggtgtgat cgcctttgcc
     4981 gatattggtg atcagtgcac tgtattgatt ttgcaggata tcctgcaatt cgcagcagtg
     5041 aacggtacga ccaataatac ggcccagcgt ggagtgcagc tgcgccactt ccagcgtgtt
     5101 gccagtcagt ttctgataaa tcgcaacgat ttcattcagt ttgttctggg tagattcgcg
     5161 acctgccgcc agtttcacca gcatattagc cagcggaccc acttctaccg ttttgccgta
     5221 gaaagtcggt gatttcaccc aggaatattt cccgtcgtca gaccaaccat cataagccgg
     5281 aatggtggta ccttcccacg gtgcctgcgg cgcttcgtct ttataccagg agtgcttcgc
     5341 gctttcctga atgcctttga tcaggtattc atcggaatga gaagtgatcg gacgatacga
     5401 ggacagatcc gcattctcaa tgtagccgcc cgggaacagg aagctgccgt ttttactgtc
     5461 ggtcgggaat tccggcacgc tcaggtagtt caccgcacct ttaccgcgcg tcagccattc
     5521 cgggtagaac gcggcaataa ctgcggtatc aaccttataa acctgttcaa caaagtcgct
     5581 cagcttgtcg atgaaagact tgatgtacat caggcgctca aggttcagca cgcccaaacc
     5641 gtcgaggttg atcgggttcg cgacaccacc taccgccagg ttctgaatgt gcggcgtttt
     5701 accgcccagc agcgccacga cgcggttagc gtcacgctgg cactccagcg cctgcaggta
     5761 gtgcgctacc gcaatcaggt tcacttccgg cggcagtttc atcgccgggt gaccccagta
     5821 gccattggcg aaaataccca actgaccgct ggcaaccaga tctttgatct tgttctgaac
     5881 tttagtgaac tcttccggac tgttcagatg ccaggtcgaa acgcctttca gcatttcgga
     5941 ggctttggtt gggtcagctt gcagtgcaga agtgatgtcc acccagtcca gcgccgaaag
     6001 ctgatagaaa tgaacgatat ggtcatgcgt ggtgtgcgca gccagaatga tgttacggat
     6061 gtattgcgcg ttaaccggaa cgtcgatatt cagcgcactt tctgccgcac gaacggaaga
     6121 cagcgcgtga gtggtagtac atacgccaca gatacgttgc acaatcatcc atgcatcgcg
     6181 cggatcgcgg tttttcacga tctcttccat gccgcgccac atggtaccgg aagcccatgc
     6241 tttcgaaacg acgccatttt cgatttcgca atcgatgcgt aaatgcccct caatacgggt
     6301 taccggatca atagtaattc tctggctcat gctttgctcg cctcatgacg attatgatcg
     6361 ttttgtttta aaggaggaag tatcggcagt agacgaatga gtacgatgta agcgcaaatc
     6421 tcaatagcca caaaaccaat agaaatcaac agttcttccc aggtcgggaa gtaggcgtaa
     6481 ccgccgcccg ggttgaatgc caccagcgaa taggtcagac gccaggttgc acaacctaac
     6541 agtgcgctca gtgctgacag gaacagcatg cgggaatcat tacgcagctt cgccacacgc
     6601 agaacgacca gcgggaagag catcagcagg acttcaatcc agaacatcac ggagtagaag
     6661 tcaccggcaa acgctaacga cagcttgtcg cgatagataa gctcgccaaa gcgcagcacg
     6721 atgaaaatcg ccagcaacac actgatggtg ttggtcagct tgacgaacag actcttttca
     6781 tccggaccgt tgccacgcag acccgcctgc accagcgaac cttcaaagat gacaatcgag
     6841 aagcccatga tgaacgccgt cagcagcgag aacagcggca acatttcata gctctgccac
     6901 aacggatgca ccttgtagcc cgccgagatc atcagtgacc ccattgaaga ctggtgcatg
     6961 gtcggcaaca gcgcgccaag tgcaatgatg aagaacataa ccttgttcag acgcttgaga
     7021 gaaaccttcc agcccagacg ttcaaacagt gccggagcaa actccagtgc catcacgccg
     7081 atatagatgg tcatacagac cgccgtctcg aacagtaccg agttcacgtt gaagtgaccc
     7141 ggaatgtaga agtacggcag gttccagtag cgacccacgt cgatagtgat cgacaagcca
     7201 cccagtgagt aaccaaacag actcgccaac agcgccggac gcaccagcgg atggtattgc
     7261 ccacggttaa agacgtatac cgcccacgcc agcgcccagc cgccacaggc aaagccggtg
     7321 ccaatcagca ggtcaaacgc gatccacacg ccccacggga agccgccgtt caggtcagag
     7381 acagagccca gaccgaacac cagacgcttc acaatcagga gcatacagat gacgattaac
     7441 ggtccaaaaa tcatgaccgg tttactgatg attttgccgc ccagcggttg tggatcatga
     7501 ctcatgatcg tctcctccgt cgtgatggtc gtttttggtg ttgcgacgaa ccagcacggt
     7561 taagcccgcc agcacagcca gtggtagcat catgccttta tacagggtgt gttgaatatt
     7621 ttcggaacgc gcaccggtag aaagatcgtc cagtttcggc aggtcgagat tttcataagg
     7681 cacacccgtc agtaccagaa cctgagtacc gccgccctct ttctcgccgt acagatgcgg
     7741 ataatatttc ggcaccgtat gcaggtaagt gtcgccagat ttcagcgtct gacgcggata
     7801 gtggtattcg ctgccaggct tcagcgccag acgttttttc gcctccgcca tcagctcttc
     7861 acgcgtaccg aaaatcaccg cgcccgccgg gcacacttct acgcagccag gcagaccgcc
     7921 tttatcgaga cgttccacac ctttctggtt gcacagctcg cacttatgca gcgcaccaaa
     7981 cgggttgttg tagtcgtact tcggcacgtt gtacggacag gcgaccatgc agtaacggca
     8041 accggtgcac acgtctttgt cgtaatggac aatgccggtt ttcggatctt ttttcagtgc
     8101 agagaccggg cacacagaga cacagttcgg atcgacgcag tgcatacact gtttcttaat
     8161 gtacgcgtag ccgttctcct cctggtcttt gttgacccct gtgccgctgg tccacacctg
     8221 aatgatgtta ttggtatacg gcgacagttt gtcgttgttc gaccaggtct gttccccttg
     8281 cgggttacgt tcagggaagt tgatatcctg acacttggtg acgcaagcct ggcagcctac
     8341 gcacaaggtc gagtcgtaca acatccccag cgatcccgga attggcgggc ggttttcagc
     8401 agccgcatga ctgacagacg gcagcgcgcc cgtcagcaat gccccgcagg aggctgcttt
     8461 aataaaatta cgtctgttca cggttattct ccccgtgagt cagcgttatc tttcttttgc
     8521 tgacgaccca gttcacgcac cgccatcacg ctgacaccgg caaccagccc aaccacaccg
     8581 ccgagcaaac caatagcgcc tgcagagacg ttgccgccct ctttagcgtt aacatccggt
     8641 ttctgtgaac gcggagtctg attttcgacg ttggcaagct gatggatgcc tttatggaag
     8701 ccgatacctt cttcgttaca gccatagcat gggtgaccaa tcgctaccgg ccacacgccg
     8761 ccaacatcgc agaattgcag cgttgagcag ttgccgtaag tttctggccc tttacagccg
     8821 aggtggtaca ggcaccagcc ttcacggtgg ccttcatcac cgaactcttt ggcaaaacga
     8881 ccagcatcga agtgcgggcg acgttcgcag tgttcgtgaa tcagacggcc ataggcgaag
     8941 gtcggacggt ttttgtcatc cagtttcggc ggtttgccgt aagtgatgat gtgcgcaacg
     9001 gtcgcgagga agttgtgcgg gttcggcggg cagcccggaa tattgataac ggttttgcct
     9061 ggcagaactt cttgcaggct gactgcgcca gttgggttaa ctccagctgc ggcaacaccg
     9121 ccccacgcag agcaggaacc gatagcgata atcgctgctg cgccttctgc cgctttgcgg
     9181 atgtgatcca caatcggctc accagcaacc atgcaataaa taccgttatc ttttaatggg
     9241 atggaaccat ccacaaccaa cacatactgc cctttgtact tctcaagtgc gttatgtttg
     9301 ttctcttcga cctgatgacc gaaggcggcg gaaagcactt cgtgatactc cagagagata
     9361 gtctccagca cgaggttttc taccgttgga tgcgttgcac gaagcagaga ttccgtacaa
     9421 ccggtgcact cctgcgcgcc aatccagata actggcggac gctgcgggtt agtaaccgat
     9481 tcggccatct ctgcagcggc tttgctactt aaccccatgg tggcggctaa tgctgcacaa
     9541 agcttcatga aatcacgacg gttaatgccg tgagaatgga tgagggtgtt atctccagtc
     9601 atttatagtt attccgttgc gaagacctgg catatatttt gcctcaatcg caaaatcaat
     9661 aatgcgatcg atgcgccatt taccacacat ttattatggt tatcgatatc atgatactgc
     9721 ggcggggcgc taaaacgaag ggaagaagca ttagtgtaat taattaaagc aagataatac
     9781 gtatgtttga tcaattttcg aggcgataac cggccataaa aaagcgcaac gtagaacagg
     9841 aattattctc acgaaagaaa tccattacca tctctttatc cagtccatag cggcgcgtca
     9901 gaatacccgc ttcccaggcg ctaagctggc gatctccggt cttttcgtac atccggtgtg
     9961 agtagcctaa tacaaaaccg cgtttataat cggcacagaa ttttgtcaca ttaaccgcac
    10021 tgtcggcatg cgttgctttt aatcccgcca ttaagccttt accaaaatgg ttattcatcg
    10081 ttcacctcat tcgctatata tcgccatata tagcgatatt ttaggcaaag aacgactttt
    10141 gcgcacgcta ttttttcatg cttgtttaag acgtaatcca gtaagagata aacctatcag
    10201 gagagatatt tatcgaacca tttcagtgtc ctttgccagg caagatcggc ggcagatttg
    10261 tcataacggg gcgtggaatc attatggaat ccgtgattaa cccccggata gatatacgcc
    10321 tcataaacct tattattggc tttcaacgcc gcctcgtaag caggccagcc ctcgttgatt
    10381 cgggtgtcca gttccgcgaa gtggagtagt aaaggcgctt caatcttcgc cacatcggca
    10441 gtgggtgcct gacgaccata aaacggcacc gcgcaggcca gttccggata cgcgacagcc
    10501 gccgcgttcg atacgccacc gccatagcaa aatccggtaa tacccacttt gcctgccgct
    10561 tgtggatagc gttgcataaa ctcaattgcg gcaaagaaat cattcatcag tttggttgga
    10621 tcaacctgct gttgcagctc acgaccttta tcatcatttc ccggataacc tccaacggaa
    10681 cttaagccgt caggtgccag ggcgatatac cccgccttcg ccactcgccg tgccacatct
    10741 tcgatatacg gattcagtcc acgattctca tgcaccacca ccacggctgg cgttttgccg
    10801 ctcatttttg cgggtttcac cagataaccc cgtacctcgc cgtgaccatt tggcgaagga
    10861 tacatgatgt actcagcaac aatctccggg tcggtaaact ctacctgagt cgccagcgca
    10921 taatttggct tgagcaaatc aaacaacgct aatgccgtca tcccgcccac cgcatacttc
    10981 gccgcaaggt tgaggaactc acgtttcgaa attttcccgt gagcgtaata gtcgtagtaa
    11041 tccaacaact cttgtgggaa atctttggcg gttaaacgcg gcatcgttgc actcctcagt
    11101 tggtgttttt ttaagcaaag cataagcacg tgtttttgcc cagtttttcg tcactctgtg
    11161 agccagacta cgggatacgc gctggcgaat cgctaaacta gaaacattgt ttcgaaattg
    11221 aacggtggaa aggagaggtc atggtctggt tagcgaatcc cgaacgttac gggcagatgc
    11281 aataccgcta ttgcggaaaa agcggtttac gcctgcccgc gttatcgctc ggtttatggc
    11341 acaatttcgg tcacgttaac gcgctggaat cacagcgtgc gatcctgcgt aaagcgtttg
    11401 atttgggcat tacgcacttt gatttagcca acaattacgg gccgcctcca ggaagcgcag
    11461 aagagaactt tggtcgcctg ctgcgggagg attttgccgc ttatcgcgat gaactgatta
    11521 tctctaccaa ggctggctac gatatgtggc ccggccctta cggctctggc ggttcacgta
    11581 aatacctgct cgccagcctc gaccaaagcc tgaagcgtat agggcttgag tatgtcgata
    11641 tcttttactc tcatcgcgtc gatgaaaata cgccgatgga agaaaccgcc tctgcgctgg
    11701 ctcatgcggt acaaagcggt aaggcgctgt atgtcgggat ctcctcttac tcgccagagc
    11761 ggacgcaaaa aatggtcgag ttgctgcacg agtggaaaat tccgctttta attcatcaac
    11821 cttcgtacaa tttactgaac cgctgggtgg ataaaagcgg cctgctggat accctgcaaa
    11881 ataacggcgt gggctgtatt gcctttactc ctctggctca gggattgctg accggaaaat
    11941 atctcaacgg cattccgcaa gattcacgga tgcatcgtga agggaataaa gttcgtggtc
    12001 tgacgccgaa aatgcttacc gaagccaacc tcaacagcct gcgcttattg aatgaaatgg
    12061 cacagcagcg tggacaatca atggcgcaaa tggcgttaag ctggttgctg aaagatgatc
    12121 gcgtgacgtc ggtattgatt ggtgccagcc gcgcggagca actagaggag aacgtgcagg
    12181 cgctgaataa tctgacattt agcaccgagg agctggcgca gattgatcag catatcgccg
    12241 atggcgagct gaatctgtgg caggcgtcgt ccgataaatg acctgttaat aacgggcgcg
    12301 cgaaccgcgc ccgaatcaga tcagtgatta tgacgagtca gtcggtcaag atagcccatc
    12361 acaaatgcag aaagcacaaa cgtcagatgg ataatgacgt accacatcag tttgttatcc
    12421 gggacatttt tcgcatccat aaagacgcgc agtaagtgaa tggaagaaat tgccacaatc
    12481 gacgctgcta ctttgttttt cagcgacgtt gcgtccattt tccccagcca gttcagcttc
    12541 tctttgttct cggagatatc cagctgtgag acgaaattct cataaccgga aaacatcacc
    12601 atcaccagca aaccgccaac cagcgtcata tccaccagcg acagcaacac gaggatcaaa
    12661 tctgattccg ccatcgagaa gatattcggc agtacgtgaa taatctcctg gaagaacttc
    12721 agcgccaggg caactaacgc cagcgaaagg ccaaagtaca cgggggcaag cagccagcga
    12781 gaagcataca ttgcattttc aagaaaacgt tccatacatt cctgtcgtgt agttaaaacg
    12841 ggcaacagta tatcgcaatt gagtaaacag cacgcaacag caaatcaaca tatacgttaa
    12901 acctttgtcg cataattctg aggtttaggg atcttcctgg ttcccttatc aacgcatcga
    12961 gagcgtctac ccttaaagga cgttgaacct gaagggagaa aaacgatgtc tcatttaaaa
    13021 gacccgacca cgcagtatta cactggtgaa tatcccaaac agaaacaacc gacgccaggc
    13081 atccaggcga agatgacacc ggtaccggat tgcggcgaga aaacctatgt tggtagcggt
    13141 cgcctgaaag atcgtaaagc actggtgaca gggggcgatt ccggaatagg tcgcgctgcc
    13201 gccatcgctt acgcgcgtga aggggctgac gtggcgatca gttatcttcc cgtggaagaa
    13261 gaagacgctc aggatgtgaa aaagatcatt gaagaatgcg gacgcaaagc cgttctgctg
    13321 ccaggcgatt taagcgatga gaaattcgcc cgttcgctgg ttcacgaagc gcacaaggcg
    13381 ttaggcgggc tggatattat ggcgctggtc gccgggaaac aggttgccat tccggatatt
    13441 gcagacctca ccagcgaaca gtttcaaaag acctttgcca ttaacgtttt cgcgctgttc
    13501 tggctaaccc aggaagcgat ccccctgcta ccgaaaggtg caagtatcat caccacttcg
    13561 tcaatccagg cataccagcc aagtccgcat ttactggact atgcggctac gaaggcggcg
    13621 attctgaact acagccgtgg cctggcaaaa caggtcgcgg agaaaggtat tcgggtgaat
    13681 attgtcgcgc caggccctat ctggacggca ctgcaaattt ccggcggaca aacgcaggat
    13741 aagatcccgc agtttggtca gaaaacgccg atgaagcgtg cggggcaacc ggcggaactg
    13801 gcccctgtat atgtttatct ggcaagtcag gagtcgagct acgtcaccgc agaagtgcac
    13861 ggcgtgtgcg gcggcgagca tttaggttaa aaaaatgccc ggttgtgaaa agcaaccggg
    13921 catcattgtg aattcactct tacctgatgt ggcgaaaacg ccttatccgg cctacaaacg
    13981 cacgaagatt caacatgttg caatagattt tgtaggcctg atgagcgtag cgcatcaggc
    14041 aattctactt tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc
    14101 cgcctgatgc agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttgtccgc
    14161 tcggaagaag atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat
    14221 tgtttcatcg gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag
    14281 ataaaccggt ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt
    14341 cacatctacc gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac
    14401 gtcgataaac ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt
    14461 aagatgcatt gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg
    14521 cgcagcggcg ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc
    14581 aacatcaccc agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac
    14641 gaccgcagga atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc
    14701 cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat
    14761 gatgccccag accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag
    14821 atagccgtta ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa
    14881 gctggtacgt tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt
    14941 ctgcgcttca ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc
    15001 gttggcctgg tttaaggaac gcgcttcagc cagcagttgc tgctcgcgct taaggcgacg
    15061 cttctgattg aagaactcta cgctcttact gaagaagatt gcccaagtga ctacggaggc
    15121 caaaataagc ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca
    15181 gacggaaagg tccgtctgca tcaaattatt acccactgtg tatctccagg acgcaagtca
    15241 caaaatctgc gcataataat atcaaaatga cgtcgaattg atagtcgttc tcattactat
    15301 ttgcatactg ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca
    15361 gcacgccatt tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata
    15421 ttcatgctag tttagacatc cagacgtata aaaacaggaa tcccgagatg acggacaaaa
    15481 agcttgatac tcaactggtg aatgcaggac gcagcaaaaa atacactctc ggcgcggtaa
    15541 atagcgtgat tcagcgcgct tcttcgctgg tctttgactg tgtggaagcc aaaaagcacg
    15601 cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt
    15661 tctccttaca acaagcgatg tgtgaactgg aaggtggcgc aggctgcgtg ctatttccct
    15721 gcggggcggc agcggttgct aattccattc ttgcttttgt cgaacagggc gatcatgtgt
    15781 tgatgaccaa caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac
    15841 tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc
    15901 agccaaacac taaaatcgtg tttctggaat cgccaggctc catcaccatg gaagtccacg
    15961 acgttccggc gattgttgcc gccgtacgca gtgtggtgcc ggatgccatc attatgatcg
    16021 acaacacctg ggcagccggt gtgctgttta aggcgctgga ctttggcatc gatgtttcta
    16081 ttcaagccgc caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtat
    16141 gcaatgcccg ttgctgggag cagctacggg aaaacgccta tctgatgggc cagatggtcg
    16201 atgccgatac cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc
    16261 aacatcatga aagcagtctg aaagtggctg aatggctggc tgaacatccg caagtagcgc
    16321 gagttaacca ccctgctctg cctggcagta aaggccacga attctggaaa cgagacttta
    16381 caggcagcag cgggctattt tcctttgtgc ttaagaaaaa actcagtaat gaagagctgg
    16441 cgaactatct ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat
    16501 cgttgatcct ggcgaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg
    16561 attttagcgg gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg
    16621 ccgatctgga cgccggtttt gcgcgaattg tataacattg ccacttttgg acaattttgc
    16681 agacatttaa ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga
    16741 atcaggagta caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct
    16801 gttattcaag atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct
    16861 catattgtta gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg
    16921 ctgcccgcct catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc
    16981 caaggggtta tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc
    17041 tgctggctaa gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg
    17101 ctggcacagc ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt
    17161 ctgctggcgc tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg
    17221 gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg
    17281 ctgtgggtca gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa
    17341 cgccatgaag atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct
    17401 ggcttgttag gcacgctgtt tgtagtgatt aaaaaaaaat actgtaacgc ctgacgattt
    17461 tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct
    17521 tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga
    17581 tgcgctttca tagtcgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat
    17641 catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt
    17701 gacagactta aaattatgat ggaacgccga tacgctcatg ttggcttctg ccgccagttg
    17761 ctcgacgctc aggttttcgg tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag
    17821 actgaagtga gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag
    17881 cacgtagtac agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg
    17941 ctccatcacg tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga
    18001 gttaatcccg ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa
    18061 cagttcctgt aactgcaaaa tatcgacatt gagacgcaat cctgccagcg gcacctctga
    18121 cgtggcatag gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc
    18181 atcataacga aacacgcgtt cattgatata accgatttta tgcccggaaa agagaattat
    18241 gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg
    18301 cacatcgggc aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc
    18361 cgccagcaag cggcaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg
    18421 gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga
    18481 cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg
    18541 cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa
    18601 tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca
    18661 aattcctcac gatgctcgcg tattgattac ctacggcggc ggcagcgtga aaaaaatcgg
    18721 cgttctcgat caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat
    18781 tgagccaaac ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa
    18841 agtgactttc ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc
    18901 cgcagcggct aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa
    18961 agagattaaa agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga
    19021 atccaacgca ggcgcggtga tctcccgtaa aaccacaggc gacaagcagg cgttccattc
    19081 tgcccatgtt cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc
    19141 gcgtcaggtg gctaacgacg tagtggacgc ctttgtacac accgtggaac agtatgttac
    19201 caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctaat
    19261 cgaagatggt ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat
    19321 gtgggcggcg actcaggcgc tgaacggttt gattggcgct ggcgtaccgc aggactgggc
    19381 aacgcatatg ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact
    19441 ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgag accaagcgcg ctaagctgct
    19501 gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc
    19561 cgcgattgcc gcaacccgca atttctttga gcaattaggc gtgccgaccc acctctccga
    19621 ctacggtctg gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat
    19681 gacccaactg ggcgaaaatc atgacattac gttggatgtc agccgccgta tatacgaagc
    19741 cgcccgctaa gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag
    19801 ttcacaacac ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct
    19861 aatccaaccg ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc
    19921 tggcaagcaa gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat
    19981 cgctcgattg ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa
    20041 aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac
    20101 aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac
    20161 ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc
    20221 atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc
    20281 catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa
    20341 cttcatccgc tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag
    20401 accgaatcct ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt
    20461 cgcgatctgg cagataaata cggcaaagcc ccggcgcaga ttgttatccg ctggcatctg
    20521 gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt
    20581 gatgtctggg atttccgtct cgacaaagac gaactcggcg aaattgcaaa actcgatcag
    20641 ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct aacatgcaaa ttctcccggt
    20701 gacggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc
    20761 gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga
    20821 agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca
    20881 gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcgcagcc
    20941 tccttcacag accgtagaac aaacagtccg gcagatttat cagaactata aatcagatgc
    21001 cagtacacct tatttcggtg aaaccggaga gcgggcgata acttcagcgc gtattcaaca
    21061 ggcgcttacc ctgaacgaca atcttacgct gccgggcaat attggctggc tggattatga
    21121 tccggtttgt gattgtcagg attttggcga tctggtgcta gaaagcgttg cgataaccca
    21181 aactgacgcc gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga
    21241 aaagaccacg cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgacgatat
    21301 tgtcagcaat catggcagcg tcttacaagc agttaatagc gagaatgaaa aaacgctggc
    21361 cgctttagct tcgttgcaaa aagaacagcc ggaagccttt gttgccgaac tctttgaaca
    21421 tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt
    21481 taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat
    21541 agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg
    21601 cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt
    21661 tacgctgacc aatggcaaca acgaagagca agaactggtt ttacagcggc gcgaaggcaa
    21721 gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc
    21781 aaaaaccgcc gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata
    21841 aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc
    21901 tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc aactggcgag caatgtacaa
    21961 acattaaatg ccaaaatcgc ccggcttgag caggatataa aagcactacg cccacaaatc
    22021 tatgctgcca aatccgaagc taacagagcc aatacgcgtc ttgatgctca ggactatttt
    22081 gattgcctgc gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gcagcatgtc
    22141 agttgccggg gatttttttt aacgtccaac cgccgcttta gggcgtttct tcgcaccagc
    22201 attcaccgga cgagattgcg tagacgacgc tttttttgcc gtagcaggcg tctgacgctg
    22261 ggtcgccatc ggcgtatgtt tagtcaacgc cggacgggta ttgcggttct ggcgacgagc
    22321 ttcacgcatc tcttcaatgg ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag
    22381 atgctttttg cccatcgctt ccagcgccgg gcggattaac ggccagtttg ccggatcgtg
    22441 gtaacgcaac aacgctttat gcaaacgacg ctgtttgtcg cccttcggta cgaagacgtc
    22501 ttcgctctta taaccaatct tcgccagcgg ttttttcccg gtgtaataca tggtggttga
    22561 gttcgccagc ggcgatgggt agaagttctg tacctggtcg agacggaaac gatgcttttt
    22621 cagccacagc gccaaattca ccatatcttc atcacgcgta ccggggtgcg cagagatgaa
    22681 atacgggatc agatactgct ctttacctgc ctgtttcgag taggtatcga acagctcttt
    22741 aaagcggtca tagctgccca tgcccggctt catcatcttc gacaacggcc cttcttcggt
    22801 atgttccgga gcaatcttca gataaccgcc gacgtgatgg gtcgccaact ctttgatata
    22861 gcgcggatct tccacggcga tgtcataacg tacgcctgag gcgatgagga tttttttaat
    22921 gcctttcaga tcacgcgcac ggcgatagag gttgatcgtc ggttcgtggt tagtgtccat
    22981 gtgcggacaa atatccggat aaacgcacga caaacggcga caagtttgtt cagcgcgtgg
    23041 cgatttgcag tgcaacatat acatgttggc agttggccca ccgagatcgg aaatcacgcc
    23101 cgtaaaacct ggaacggtgt cgcggatcgc ttcgatctca ttaatgatcg aatcttccga
    23161 acggctctga ataatgcgcc cttcgtgctc ggtaatagaa cagaaagaac agccgccaaa
    23221 gcagccgcgc ataatgttga ccgaaaaacg gatcatttcg taagccggaa tacgggcatt
    23281 gccataggcc ggatgtggca cgcgcttgta cggcagcgca aaaacgctgt ccatctcttc
    23341 ggtagaaagc gggatagcag gcgggttgat ccacacatag cggtcaccgt gtttttgcat
    23401 caatgcgcgg acgcagcctg ggttggtttc gtggtgcaga atacgcgaag catgggcgta
    23461 cagcacttta tcgcccttca ctttctcgaa agaaggcagc aacacgtagg ttttttccca
    23521 cggtttcggg cgcggtggct gcacggttac ggctttggct tcctgctttt tcggtgccac
    23581 cggtttgtta tccgcgcacg gcaaatcttc accatacgga tgcgggattg ggtcgatttt
    23641 tccaggggta tcaagacggg gggaatccac gccgctccag ccaggcagcg cctctttcac
    23701 gataatcgcg gtattacgca catcgcggat ttcactaatc ggctcgccca tcgccagacg
    23761 gtgcgccacc tccaccagcg gacgctcacc gttaccaaac atcagcatgt cggctttcga
    23821 atccaccagc acggaacggc gcacggtatc ggaccagtaa tcataatgtg cggtacggcg
    23881 cagactagcc tcaataccgc cgaggatcac cggtacatct ttccacgcct ctttacaacg
    23941 ctgggtataa accagtgtgg cgcgatccgg gcgcttaccc gcgacgttat ccggcgtgta
    24001 ggcatcgtca tgacgtaaac ggcgatcggc ggtataacgg ttgatcatcg aatccatgtt
    24061 gccagcagta acaccgaaaa acagattcgg tttacccaga cgcataaagt cgtctttgct
    24121 gctccagtcc ggctgggcga tgatcccgac gcgaaagccc tgtgcttcca gcatacgacc
    24181 gcaaatcgcc atcccgaagc ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat
    24241 gtcgcagcta tcccagccaa gttgatccat ctcttcacga gacatcggca aaaacggtgc
    24301 cggtccaaaa caggcggccc agtactgcgg ccaggagaac aggtcgcgat ccggttggat
    24361 cagggagata gagctcattt tgcttccaga aatgataaaa aaataatcaa aggccgggga
    24421 ttataagccg gaacgaaaga gaaatcgaaa agtattccat actcgccctc ctcgggcgag
    24481 tatgaagatt acggtaccgg attgactaac agttgcccaa tcgagccacg gtccgccatt
    24541 tccagcgtct gactgttgaa gtagaacggg aagtgcgccc aggaaggctg accgaaataa
    24601 acaagcagct ccacttgtcc gtcaacccaa acggtatctt tccagcctct gtcttccggg
    24661 aacggcatcg caccattcac gttacggatc tggaacatca cgccttcaat atggaacgct
    24721 tgcggctcgt ccgcgcgtac cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca
    24781 atacggttga cgtcccacag ctgtccatta atacccgggt catcacccag actgatatcg
    24841 cgactgcgaa tcggcgaacc ggccatgatt tcagttggca gcaagcgcat cggaagactg
    24901 tctgtgacca gcggcagaag gccggttggg cgcagcgtta gcaccagggt agaaaccaga
    24961 atactggatg gctcaaagaa gccgcgaata cgatcaacaa tgctcgccgc ttcgccacag
    25021 gtgatcgaca cttcatcgcc gttgctcata tccaccagaa tctcgcggcg ctcgcccggt
    25081 gccagcgaaa gttgcttcac cgatacagga gcaggcagga atccctgatc gccagaaatc
    25141 acatgtaacg ggcgaccatc gctcatctgt agttgatagc gacgagagtt cgacgcgttc
    25201 agcagtcgca agcgcaccca gccacgcgag acttcaacgt acgggctttg tacaccgtta
    25261 accagcagcg tatcaccaac aaagccgccg cttcccggtt cgttgtattc tggcgtacca
    25321 aagttatcca gccgtttatc ctggataatg accggaaaat catccacatc ataatggttg
    25381 gggataggca gcgacttgct gacttcatct tccaccagcc acattccggc aaggccgtta
    25441 tagacctgct gagccgtgcg gttgggagta ttggcgtgat accacagagt agctgcgttc
    25501 tggcgaatag gcagtacggg tgcccagtca gcgtttggcg acatcatccg tgccggaccg
    25561 cccatcagcg ggccaggtac ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg
    25621 cggttgctgt aaataagctt aacatcgtcg cccttccaga cgcggatagt cggccccagg
    25681 taacgaccat tgattcccca gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca
    25741 cgttgtacag tcataaacag cggttgccca cggcgagatt caagtagcgg cggaacgggt
    25801 agcggttgct gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca
    25861 atccccgatg cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc atgtaaaact
    25921 ggctaaagta ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa
    25981 cttttccggc ggcttcgcgt tctgcgactt ctttatcgag ctcggcgatt ttttgttcca
    26041 ttatcgaacg acaatgggca gccagctcac gaacctgatc tttgccatac tgactgacgt
    26101 caattggcgg cagcatttcg acaatcacca gaccgttgtg cagtcgatta agattaatct
    26161 tattcgaagt tgtagagacg cacacgggaa taatcgggac gcccgccgca attgccgcgt
    26221 gaaatgctcc agtcttgaac ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga
    26281 acatccagat ggaaatacgg cgttttttga aatgattcac gacttccgca atggtgccgt
    26341 gagctttagt gcgattgttt ctgtcgatca ataagttgcc ggttaaccag tacaactgcc
    26401 cgaagaaggg gatccacagc aagctctttt tacctaccgt caccgtcggc ggttgcacaa
    26461 tgttcgatgc tgtcaccatg tcatagttgt tctggtggtt agcgatatag atagcattgc
    26521 cgtagctttc agcatctgca ggtttgcggc actcaacttt caggccgaac agcggcgcaa
    26581 gacggccaaa catatgccca aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc
    26641 agtaaatgga gccgaataca cagactaaga tgctgtaaat cacggtaata ataagacgaa
    26701 agatatatag cataacaccc tctgaaggtc tgagagactg gcattgtacg tcacctgaaa
    26761 tcaggttagg gcattgttaa aagccgctct ctggaaaagc gtattgttaa tcgcaacgca
    26821 tgaataaaca acggtttcaa gggaattttt atcgtttccg ggggttgaat acgctgccgg
    26881 atgacgactt aacgtttcat ccggcgttcc ttgcaagcgg gaggaaacag gccctccccg
    26941 gcatattact cttcgctatc accgttgctg gcacggcgag gagagtcgat ctcaacacga
    27001 tcgatacgct gcaaaccgcg catcaacgta ccgcggcgtc cacgttcacc agtgactttc
    27061 tgtaactctt ccgggcgcag tttaattttg cgtttcccaa catgaatggt cagcgtgctt
    27121 tgcggcggca gaacgtacaa ttgcgccaga ccatcttctc cacgcgcggc ttctgccgat
    27181 ggaatgttga taatcttgtt gcctttgccc ttcgacagct gcggcagatc gcttaccggg
    27241 aacatcaaca tacggcctgc ctgagtgatt gccagcagca tatcggaagc atcttcaatc
    27301 accaccggcg gcataacatg ggcattttcc ggtaaggtga tcaaagcctt acctgcacgg
    27361 ttacgcgcca ccagatcgtt aaaggtgcag acgaaaccgt aacccgcatc ggaagccatc
    27421 agcagtttct gatcgtcgct ttccatcagc atatggtcaa cggtcgcccc aggcggcaac
    27481 gttaatttgc cggtgagtgg ttcgccctga ccacgcgccg acggcagcgt aatcgggtcg
    27541 atggcatagc tacgaccggt ggaatcaaca aacactaccg attggttgct cttacctttc
    27601 accgccactt tgaagctatc acccgcttta taattcaggc ccggcgcgtc gatatcatgg
    27661 cctttagcgc tgcgtaccca gcccatctgc gacagcacaa tggtgacagg ttcagacggc
    27721 agcatgtcgt gctcgctcat cgctttcgct tcttcgcgtt cctgcaacgg cgaacgacga
    27781 tcgtcaccgt aggcttgcgc gtctgcctgc agttctttct tcagcaggtt attcatttta
    27841 cgctcggaag ccaaaatgcc ctgcaactgg tcgcgctctt tttccagctc actctgctca
    27901 ccgcgaatct tcatctcttc cagtttggca agatgacgca gtttcagttc gaggatcgct
    27961 tccgcctggg tttccgtaag gccaaaccgc gacatcagcg ccggtttcgg ttcatcttcg
    28021 ttacgaatga tctcaatcac ttcgtcgatg ttgagaaacg ccaccagcaa accttccagg
    28081 atatgcaggc gcttgaggac tttctccaga cgatagttca gtcggcggcg cacggtatcg
    28141 cgacggaaca ccagccattc ggagaggatt tccagcaggt ttttcaccgc cggacgacca
    28201 tccagaccga tcatattgag gttaatgcga tagctctttt ccagatcggt ggtagcgaag
    28261 aggtggttca tcacctgatc catatccacg cggttggaac gcggcacaat caccagacgg
    28321 gtcgggttct cgtggtcaga ttcatcgcgc agatcgtcaa ccatcggcag ctttttgttg
    28381 cgcatttgcg cagcaatttg ctccagtaca cgcgcacctg aaacctgatg cggtaatgcg
    28441 ctgataacca ccgcgccatc ttctttcttc cacaccgcgc gcatacgtac tgaaccacgt
    28501 ccgttctcgt agattttacg gatctcggcg cgcgaagtga taatttccgc ttcagtcgga
    28561 taatccggcc cctgcacgat atccagcagc tgatcgagcg tggttttcgg ctggtcgatt
    28621 aatgcgattg ccgcctgagc tacttcacgc aggttatgcg gtggaatatc ggtcgccatg
    28681 ccgacggcaa taccggtggt gccgttaagc aaaatgtttg gcagacgagc aggcagcatt
    28741 ttcggctcct gcaaagtgcc gtcgaagttt ggcacccagt cagccgtccc ctgccccagc
    28801 tcgctcaata gcagctcgga atatttcgac aaccgggatt cggtgtaacg cattgccgcg
    28861 aacgatttcg gatcgtccgg cgcgccccag ttcccctgac catcaaccag cggataacgg
    28921 taagagaacg gctgcgccat caggaccatc gcttcataac aggcgatatc gccgtgcgga
    28981 tggtatttac ccagtacgtc accgacggta cgggccgatt ttttaaattt ggcgctggca
    29041 ttcaggccca gttcagacat cgcatacaca atgcggcgct gaacaggttt cagaccatca
    29101 ccaataaacg gcaacgcacg gtccatgatc acgtacatgg agtagtttaa gtaggcgttt
    29161 tccgtaaatt catgtagcgc aaggcgctct gccatatcgc tcattaattc tgattcctca
    29221 actgattcgc cagccttcaa aggggcaata ttgccggaga tactacatta tctgccgcgt
    29281 cgagtcacag gagatcagcg tgaatagcgc cgccggacgt gctccgacgg cgataaaggc
    29341 atcaatgtgc cttgatatac aacgctttgc tgtagacttg atcggcatag agggcagcac
    29401 aaggttgcgc ccatgccaca ccactcggca tgggggtccg ctccgggcga ttcgagaagt
    29461 tgaggttatg cgagtccagg gaagcctaaa gcatcaaagt agtcgactgt aagtatatgg
    29521 ttcagcccag tttagctgtg tctccaccag tgccccgcgc ctcctgccat aagtgcggct
    29581 tgctcatggg tacccccgag acggcttacg gcgcaccgga taataaattt cgcgtataca
    29641 tggtattcct taccggagaa attataagcc caacaacaat acgcctgtgg cattgttcgc
    29701 tcaaacttat cgcgagtgat ttggctcaca ctccggtaaa gattccccat cacgctaacg
    29761 gcacataaac atccgtttcc agtcgctgct ccgtcacccc ttcggcaaga ttggtgtagt
    29821 gaaacagaat cggtgcttta cgcattttct cgccgcttgc aggcagccag tggcgaataa
    29881 tgccccatat cgtgtgtgaa atatcgtcca gctcgccaac gtggcgggcc acggcataac
    29941 gtccaccggt aagttcacca ttgctcacac cataacgatt atcgggaatc ggttcgctaa
    30001 cgctgccgca gatatcaaag cgaaacgctt ccggtgcggt ggtggcaggg tcgtcccagg
    30061 cgacgccaaa agtctggctt tggttaacag gagaaagccc cgtctcttta cgccacatga
    30121 taaatttcgc cacactgtag ttgagcaact cagggctaca gcggtgcggc aacatcgcca
    30181 cagggattga tgggaaatcg ataatgttta catccagtgt caggtttgtc attttgcctc
    30241 cctgcgaaat tattcccggg aacgcgtccc gggagcggta acaattacgg atttactttg
    30301 cggatctgtt tgacgtcaat ttcaacagaa ttccagtctt tatcgacttc accctgaatc
    30361 tcaaccgtat ctttcggcgt caccgtcaca ccgttccagc gtttgtggtc gatatcaaca
    30421 ttgatagtac cgctggcatc tttaaacacg tagagatcgt cagagatgcg ttcaacgata
    30481 ttgccgcgca gggttaccca ggtgtcgtca cgcagggatt ttgcgctttc tacagtcgtt
    30541 acgctgccgt tcggcccctg gaatcctccg gcctgacttt gcgttgccga tgggccagaa
    30601 aaaccgccct gctctgctgc catcaccggt gcactgcaca gggccattac tgcgattact
    30661 gctgcgaatt ttttcatgtt tattactccc tttaatgtct gtttccgagc atttaacaag
    30721 atagtcctta acaacttctt aagggaaaaa aataaaattt aatgctgtac agagcgcgtt
    30781 acaacacggt ttactggcag caaatacggt tatcgcaggg atgaaaaaat gcgaatttta
    30841 ctgatagaag atgacatgct gattggcgac ggcatcaaaa ctggccttag taaaatgggt
    30901 tttagtgtcg actggtttac acaaggtcgt cagggaaaag aggcgcttta tagcgcacct
    30961 tatgatgcgg tgatcctgga tttaacctta ccaggcatgg atggtcgcga tattttgcgc
    31021 gaatggcgag agaaaggtca gcgtgagccg gtactgatcc tgaccgcgcg tgatgcgtta
    31081 gcggaacgtg tagaagggct gcgtctggga gctgacgatt atctgtgtaa accttttgcg
    31141 ttgatagaag tcgccgccag gctggaggct ctgatgcgcc gaaccaacgg ccaggccagc
    31201 aacgagctgc gccacggtaa cgtcatgctc gaccccggca aacgtatcgc cacgctggct
    31261 ggcgaaccct taacactgaa accaaaagaa tttgccctgc tggaattact gatgcgtaac
    31321 gctggtcggg tactgccgcg caaactgatt gaagagaaac tgtatacctg ggacgaagag
    31381 gtcaccagta atgccgttga agtgcatgtg catcatctgc gacgcaaact cggtagtgat
    31441 tttattcgta ccgtgcatgg tattggctac acattaggtg agaaatgaaa tttacccaac
    31501 gtcttagtct gcgcgtcagg ctgacgctaa tctttttaat tctggcctcg gtgacctggc
    31561 tgctttccag ctttgtcgcc tggaaacaaa caacggataa cgtcgatgaa ttgttcgaca
    31621 cccaactgat gctgtttgcc aagcggttaa gtacgctcga tctcaacgaa atcaacgcgg
    31681 cggatcgcat ggcacagacg ccaaataaat taaaacacgg tcatgttgat gacgatgcgc
    31741 tgacctttgc catctttacc cacgacggca gaatggtcct taatgatggc gataacggag
    31801 aagatattcc ctatagctat caacgggaag gttttgctga cgggcaactg gtcggtgaag
    31861 acgatccttg gcgttttgtc tggatgacct cacctgatgg caaatatcgc atcgttgttg
    31921 gccaggaatg ggaataccgt gaagacatgg cgctggcgat tgttgccggg caattgatcc
    31981 cgtggctggt cgcactgccg attatgttaa tcatcatgat ggtactactg ggtcgtgaac
    32041 tcgcgccgct gaacaaactg gcgctggcac tacgtatgcg tgaccctgac tcggaaaaac
    32101 cactaaacgc gactggcgta cccagcgaag tgcgaccact ggttgagtcg ctaaatcaac
    32161 tgttcgcccg cacacatgcg atgatggttc gagaacgacg ctttacctcc gatgcagctc
    32221 acgaactacg tagcccgtta acagcgctga aagtgcaaac cgaagttgcg cagctctctg
    32281 acgatgatcc gcaggcgcgg aaaaaagcac tgctccaatt acattccggg atcgatcgcg
    32341 ctactcgtct ggttgatcaa ctgctcacgc tatcgcggct ggactcactg gataaccttc
    32401 aggacgtcgc ggagatcccg cttgaagatc tcctgcaatc gtcggtgatg gatatttacc
    32461 acacggcgca gcaggcgaaa attgacgtgc gactgacact caatgcccac agcatcaaac
    32521 gcactggaca accgctattg ctaagtttgt tggtgcgaaa ccttctggat aacgcagtgc
    32581 gctacagtcc acagggcagc gtggtggacg tcacgctgaa tgccgataat ttcatcgtga
    32641 gggataacgg ccccggtgtg acaccagagg cactggcgcg aattggcgaa cgcttctatc
    32701 gcccccccgg acaaaccgct accggcagtg gacttgggct atcgattgtc cagcgaatcg
    32761 ccaaattgca tggcatgaat gttgaatttg ggaatgcgga acaaggtgga tttgaggcga
    32821 aggtgagctg gtaagggtaa gacttttgct aaattcgcaa aagactttgc acattttgct
    32881 aattttaccg taccgctctg tgacgtacta tagtcgacaa acgtctcacc ttgaggttaa
    32941 aaaatgagca acaccctgat tatcaacggc gcgaaaaaat tcgcccactc caatggtcaa
    33001 ctgaacgaca ccctgaccga agtcgcggat ggcacactgc gcgacctcgg gcatgatgtt
    33061 cgcatcgttc gcgccgacag cgactacgat gtcaaagcgg aagtacaaaa ctttctctgg
    33121 gctgatgtgg tgatctggca gatgccaggc tggtggatgg gcgcgccgtg gacagtgaaa
    33181 aaatacattg atgatgtatt caccgaaggt cacgggacgc tgtatgccag cgatggccgt
    33241 acccgcaaag atccgtcaaa aaaatacggt tccggcggcc tggtacaggg caaaaaatat
    33301 atgctttctc tgacctggaa cgcaccaatg gaagccttca ccgaaaaaga tcaattcttc
    33361 cacggcgttg gcgtcgacgg tgtgtatctg ccgttccata aagcaaacca attccttggc
    33421 atggaaccgc tgccgacatt tatcgctaat gacgtgataa aaatgcctga tgttccccgc
    33481 tatactgaag aatatcgcaa gcatcttgtg gaaatttttg gttaactaga gctcaggctt
    33541 tagaaggagt taaccatgct taccgtaatc gcagaaatcc gtactcgtcc tggtcaacat
    33601 caccgtcagg cggtattgga tcagtttgct aaaatcgttc caaccgtact gaaagaagaa
    33661 ggttgccacg gctatgcgcc aatggtggat tgcgctgctg gcgtgagttt ccagtctatg
    33721 gcaccggatt ctatcgtgat gattgagcag tgggaaagca tcgcgcacct tgaagcgcat
    33781 ctgcaaaccc cgcacatgaa ggcgtacagc gaagccgtaa aaggcgacgt gctggagatg
    33841 aatattcgta ttctgcagcc agggatttaa tcctgccttg tttgcccggc catcctgacc
    33901 gggcaatgtt ctttccttta aacctcaatc tccgccatgt cgcctttctc ttgcaaccag
    33961 ttgcggcgat cttccgagcg tttcttcgcc agcagcatat ccatcatcgc gtcagtacgc
    34021 tgatcgtctt catcatcgat agtcaactgc accagacggc gagtgttcgg atcaagcgtg
    34081 gtttcacgca attgcatcgg gttcatttcc cccagacctt taaaacgctg gacgttcggc
    34141 ttgcctttct tgcgttttaa ttgctcaagt acgccctctt tctcttcttc cgtcagcgcg
    34201 taataaacct ctttcccgag atcaatacgg tagagcggtg gcagtgcgac gtaaacgtga
    34261 ccgtgtttca ccaacgcgcg gaagtgtttt acgaacaaag cgcagagcag cgtggcaatg
    34321 tgcagaccat cagagtccgc atccgcgagg atacagattt tgccataacg aagctggctc
    34381 agatcgtcgc tgttaggatc gataccgatc gctaccgaaa tatcatgcac ttcctgcgag
    34441 gccagcactt cgtcggaaga aacttcccag gtgttaagga tcttaccttt cagcggcatg
    34501 atcgcctgat attcacgatc gcgcgcctgc ttggcagatc cgcctgcgga gtcaccttcc
    34561 acgaggaaca gttcggtacg gttaaggtcc tgcgcggtac aatcagccag tttgccaggc
    34621 agcgccgggc cgctggtcag ctttttacgc accacttttt tagccgcacg catacgacgc
    34681 tgggcgctgg aaatcgccat ctctgccagc aactccgccg cctgaacgtt ctggttcagc
    34741 cacaggataa aggcatcttt caccacgcca gaaacgaatg ccgcgcattg acgcgaagag
    34801 agacgctctt tcgtctgccc ggcaaactgc ggatcctgca ttttcaccga cagaacatag
    34861 gcgcagcgat cccagatatc ttccgccgac agcttcacgc cgcgcggcag aatgttgcga
    34921 tattcacaga actcacgcat cgcgtccaac aggccctgac gcaggccatt aacatgggta
    34981 ccgccctgca tcgttgggat caggttgacg tagctttcgg tcagcagttc accgccttcc
    35041 ggcagccaca gtagcgccca gtccacagct tcagtatcgc cagcgaaatt accgataaac
    35101 ggtttttctg gcagcgtcgg taaaccgttt accgcttccg ccaggtaatc attcagaccg
    35161 tcctgatagc accagcgctg ttcggtgttg ttgatctcat ctttaaaggt aatctcaacg
    35221 ccggggcaca gtaccgcttt ggctttcagc acatgcgtca ggcgtgaaac agaaaatcgc
    35281 gggctgtcaa agaaggtttc atccggccag aagtgcacgc tggtaccggt attgcgttta
    35341 ccgcaagtgc cgacaacctg taaatcctgc accttttcgc cattttcaaa ggcgatgtta
    35401 taaacctgac catcgcggcg cacgttaact tcaacgcgct tcgacagggc gttaaccacc
    35461 gaaatcccca cgccatgcag gccgccagag aactggtaat ttttgttaga gaatttaccg
    35521 cctgcatgca gacggcaaag aatcaattca accgccggga caccctcttc cgggtgaata
    35581 tccaccggca tcccgcgccc atcgtcaata acttctaacg actggtcagc atgtaagatt
    35641 acatccacgc gttttgcgtg acccgccagt gcttcatcga cactgttatc aataacttct
    35701 tgccccaaat ggttagggcg agtggtatcg gtatacatcc ccggacggcg gcgaaccggc
    35761 tcaagcccgg tgagtacctc aatggcatca gcgttataag tttgcgtcat ggtttaagtt
    35821 agcaattcga gttgatcgtc agagatggtg cagaccaaga aaatcgacga tcgggttgaa
    35881 ataatcttcg aagcccgtga atgcgtggtt gccgccttct atgacagtct ggcggcagga
    35941 agcgtagtac gccaccgcct ggcggtaatc cagcacttca tctcccgtct gttgcagcag
    36001 ccagatcaaa tccggcgctt ccagcgggtc aatctgcatg actttgagat cgtaaatatg
    36061 gcgtgactct agcacatatt gctgcccggt gtatgggttc tcgttctgac cgagatagtc
    36121 cgtcagcagt tcaaacgggc gcaccgccgg gtttaccacc actgcgggca gcataaaaca
    36181 ttgtgacaac caggtggcgt aatatccccc cagtgacgaa ccgacaatac ccagcgaatc
    36241 gccgccatgt tccaggacaa tggattccag cagttctgcc gcgtcggaag gatacggcgg
    36301 caactgcgga atgatcatct caacgtcagg gtgatgttcc gccagccagt tttttaacaa
    36361 gctcgctttt gcagagcgcg gcgagctgtt gaaaccgtgt aaataaagaa gcgtagacat
    36421 cagtagcctt ctgaagcggt atcaggttgg aaacgtgtgt ccgccaggcg atgcacctcg
    36481 gtggtcagcg tgccatcagc atgtaactcg agagtacgcc agccgggcgc gatagtatcc
    36541 agcgtaaagt tggaacagtg cggcttaaac tgcacacagg tcgacggcgt tgccagcagg
    36601 cggcgaccgt tccagtcgag atccagctcc tgatgaatat gaccgcacag caagtatttg
    36661 acgtgcggaa acttcgccag cacggtatcc agttcgcccg cgttacgcag actgtgttga
    36721 tcgagccaac tacaacccgc aggtagcgga tgatgatgca gcagcagcaa cgtatggcgt
    36781 tctggcgcat cggccagttt acgttccagc cactcaagct gaaactcgct cagctcaccg
    36841 tgcggcacgc caaacacctg gctatccagc aacaggattt gccattgctc accaataaac
    36901 acccgcttcg ccggggagat acccgcatcc tgtaacgcgc tgtacatcgc gggctggaaa
    36961 tcgtggttgc ccggcagcca gacgcagggc gcacgaaaac ttgcgatgcc ttcagcgaaa
    37021 tgctgatagg ccgcagagga ttgatcctgc gctaaatcac ctgtcgcgac aatcaggtcg
    37081 aattcgtgct gatgtggacg aatcgcctcc agcaccgcct ggtagctttc ccaggtgttt
    37141 acccctaaca gagcttcgtg cttttgtgca aacaggtgag tgtcggtaat ttgtaaaatc
    37201 ctgactctgg cctcaccagc cagaggaagg gttaacaggc tttccaaatg gtgtccttag
    37261 gtttcacgat gctagtaaac cggaatcgcc atcgctccat gtgctaaaca gtatcgcaac
    37321 cagtccgcta aaaactgatt aatttgatgc ttttcgtcgc gttgatgcaa ctttttatta
    37381 ggataatcat accgcgcttt gaagcgaaaa atctgctggc ttgaacacac ttcagccacc
    37441 atcgcgtcat gatacagacg caccgtcatt gacggaaggc tccagtaact gatcgcgggc
    37501 gcagtctgtt ctattgtcac cagggtagtg tatcgggtcg attccacaat cgtcagccga
    37561 tattgtgcgt ttgccacctg atagcttaca gtttcgccgg gtgcgtcatt gcgcggtaac
    37621 aaacggcgca attgtgaaaa gttcatctcg cacaggcgca tcatttcagg aaagtcaggt
    37681 gtgtaacgct tcatttatgc ccactcattt tttaacgctt gatgatgcag ctgcagccat
    37741 tgcaaagcga tgaccgacgc tgcgttgtcg attttcccct cttctaccca ctggtatgcc
    37801 tgttcccggc ttaccacatg aacgcgaata tcttcgtttt catcagccag accatgaata
    37861 ccgcttgcgg tcgtggcgtc cacttcgccc accataattg acgaacgctc actggtgccc
    37921 cccgggcttg ccaggaaact taacaccggt ttggtccgtt tgactatcag tcccgcctct
    37981 tcaatcgctt cgcgacgggc aacatcttcc acactttcac cctcttctat catcccggca
    38041 accatctcca gtagccaggg ggtttcactg gtgtcgtatg cggcaatccg aatctgctca
    38101 atcagcacaa cttcatcgcg cactgggtca aagggtagca agactgcggc gtgaccgcgc
    38161 tcaaaaattt cccgccgtac ctcatgactc atttgcccgt tgaatagacg atgacgaaat
    38221 ctataaagat ctaatgaaaa aaagccgcga taaagtgttt ctcgtgcaat aatttctaca
    38281 tcgtttttgc caaatgtaac gggcaggttg tctggcttaa gcattgttaa tgtcctggca
    38341 ctaatagtga attaaatgtg aatttcagcg acgtttgact gccgtttgag cagtcatgtg
    38401 ttaaattgag gcacattaac gccctatggc acgtaacgcc aaccttttgc ggtagcggct
    38461 tctgctagaa tccgcaataa ttttacagtt tgatcgcgct aaatactgct tcaccacaag
    38521 gaatgcaaat gaagaaattg ctccccattc ttatcggcct gagcctttct gggttcagtt
    38581 cgttgagcca ggccgagaac ctgatgcaag tttatcagca agcacgcctt agtaacccgg
    38641 aattgcgtaa gtctgccgcc gatcgtgatg ctgcctttga aaaaattaat gaagcgcgca
    38701 gtccattact gccacagcta ggtttaggtg cagattacac ctatagcaac ggctaccgcg
    38761 acgcgaacgg catcaactct aacgcgacca gtgcgtccct gcagttaact caatccattt
    38821 ttgatatgtc gaaatggcgt gcgttaacgc tgcaggaaaa agcagcaggg attcaggacg
    38881 tcacatatca gaccgatcag caaaccttga tcctcaacac cgcgaccgct tatttcaacg
    38941 tgttgaatgc tattgacgtt ctttcctata cacaggcaca aaaagaagcg atctaccgtc
    39001 aattagatca aaccacccaa cgttttaacg tgggcctggt agcgatcacc gacgtgcaga
    39061 acgcccgcgc gcagtacgat accgtgctgg cgaacgaagt gaccgcacgt aataaccttg
    39121 ataacgcggt agagcagctg cgccagatca ccggtaacta ctatccggaa ctggcggcgc
    39181 tgaatgtcga aaactttaaa accgacaaac cacagccggt taacgcgctg ctgaaagaag
    39241 ccgaaaaacg caacctgtcg ctgttacagg cacgcttgag ccaggacctg gcgcgcgagc
    39301 aaattcgcca ggcgcaggat ggtcacttac cgacgctgga tttaacggct tctaccggga
    39361 tttctgacac ctcttatagc ggttcgaaaa cccgtggtgc cgctggtacc cagtatgacg
    39421 acagcaatat gggccagaac aaagtgggcc tgagcttctc gctgccgatt tatcagggcg
    39481 gaatggttaa ctcgcaggtg aaacaggccc agtacaactt tgttggtgcc agcgagcaac
    39541 tggaaagcgc gcatcgtagc gtcgtgcaaa ccgtacgttc ctccttcaac aacattaatg
    39601 catctatcag tagcattaac gcctacaaac aagccgtagt ttccgctcaa agctcattag
    39661 acgcgatgga agcgggctac tcggtcggta cgcgtaccat tgttgatgtg ttggatgcaa
    39721 ccaccacgct gtacaacgct aagcaagagc tggcaaatgc gcgttataac tacctgatta
    39781 accagttgaa tattaagtca gctctgggta cgttgaacga gcaggatctg ctggcactga
    39841 acaatgcgct gagcaaaccg gtttccacta atccggaaaa cgttgcaccg caaacgccgg
    39901 aacagaatgc tattgctgat ggttatgcgc ctgatagccc ggcaccagtc gttcagcaaa
    39961 catccgcacg cactaccacc agtaacggtc ataacccttt ccgtaactga cgacgacgac
    40021 ggggcttcgg ccccgtctga acgtaaggca acgtaaagat acgggttatc tgccgcattc
    40081 ttcccccttc tcgcttcaat ttcgaccagc catcctctat tctgatgggt atttaccact
    40141 ggtcccggaa gacaaaaatg aaacggacaa aatccatacg ccacgcatcg ttccgcaaaa
    40201 actggagcgc acgccatctg acaccagtcg ctctcgcggt tgccactgtt tttatgctgg
    40261 ctggctgtga aaagagtgat gaaacagtgt ctctctatca aaatgctgac gactgttcag
    40321 ctgcaaaccc aggcaaaagc gccgaatgta ccaccgcgta caacaatgcg ctgaaagaag
    40381 ccgaacgtac tgcgccgaaa tacgccaccc gtgaagactg tgttgctgaa tttggtgaag
    40441 gtcagtgcca gcaggcacca gcccaggctg gcatggcacc agaaaaccag gcgcaggccc
    40501 agcaatccag cgggagtttc tggatgccgc tgatggccgg ttacatgatg gggcgtctga
    40561 tgggcggcgg cgcgggattt gcacagcagc cgctgttctc ctcgaaaaac ccagccagcc
    40621 cggcttacgg taaatatacc gacgcgacgg gtaaaaacta tggcgcagcc cagccaggcc
    40681 gcaccatgac cgtaccgaag acagccatgg caccaaaacc ggcgaccact actaccgtta
    40741 cccgtggcgg ttttggtgaa tctgttgcca aacaaagcac tatgcagcgt agcgcaaccg
    40801 gcacctcttc tcgttcaatg ggtggctgat accgatggaa agagtcagta ttaccgagcg
    40861 cccggactgg cgcgaaaaag cccacgaata cggtttcaat tttcacacca tgtacggcga
    40921 gccgtactgg tgtgaagatg cttactacaa gttgaccctc gcccaggttg aaaagctgga
    40981 agaagtcacc gccgaactgc accagatgtg cctgaaagtg gtggaaaaag tgatcgccag
    41041 cgatgagctg atgaccaaat tccgcattcc aaaacgcacc tggagttttg tgcgtcagtc
    41101 atggctgact caccagccat cgctttattc gcgtcttgat ctggcatggg atggcactgg
    41161 cgaacctaaa ctgctggaaa ataacgccga tacaccaacg tcactatacg aggcggcgtt
    41221 ctttcagtgg atctggctgg aagatcagct taacgccggt aacttgccgg agggcagcga
    41281 ccagtttaac agtctgcaag aaaagctaat cgatcgcttt gtcgagttgc gtgaacagta
    41341 tggcttccag ttgctgcatc tcacctgctg tcgcgacacg gtggaagatc gcggaaccat
    41401 tcagtatttg caggactgcg cgacggaagc tgaaatcgct accgagttcc tctacattga
    41461 cgatatcggg ttaggtgaaa aaggtcagtt cacggattta caggatcagg taatttccaa
    41521 cctgttcaag ctgtatccat gggaatttat gttgcgtgag atgttctcca ccaagctgga
    41581 ggatgcaggc gtacgctggc tggaaccggc gtggaagagc attatctcca acaaagcgct
    41641 tctaccgcta ctatgggaga tgttcccgaa tcacccgaac ctgctgcccg cttattttgc
    41701 ggaagatgat catccgcaaa tggaaaaata tgtggttaaa ccgatcttct cccgtgaagg
    41761 cgcaaacgtg tcgatcattg agaacggcaa aaccattgaa gcagcggaag gtccgtatgg
    41821 cgaagaaggg atgattgttc agcaattcca cccgttaccg aaattcggcg acagctatat
    41881 gctgattggt agctggctgg tgaacgatca acctgccgga attggcattc gtgaagaccg
    41941 tgcgttgatc acccaggata tgtctcggtt ttatccacat atttttattg agtaatcaat
    42001 gatgccggat ggcattcgcc atccggtaat tgttaaccta tctgcaccga cagcatactc
    42061 aggctgccca tttctatacc atcgaccgga atggtaattg gctcctgccc atcccacgca
    42121 cctaagacat acaacaacgg cagatagtgc tccggcgttg ggttcgataa cgcgccacct
    42181 tcatggtcaa ggtaattcac cagaggatgc tgctctaccg ccccttgcca cgtcagattc
    42241 gctttcacat actcattaaa cgacatcgcc caaggatatg gtgaactatc accgtgccac
    42301 ttcactgtac gcaggttatg caccacgttg ccactggcga ccaacattat tccttcatct
    42361 cgcagcgctg ccagtttgcg ccccatttcg aaatgccagg cggcaggttt gctactgtcg
    42421 atactcaact gcaccatcgg gatatcggcg tcaggataca tcttaatcag cacgccccag
    42481 gaaccgtggt caaagcccca ggcttcttta tccagcgtca ccgggaccga cgctaacagc
    42541 tcaaccagat gctgtgccag cacaggcgaa cccggagcag gataatgcgt atcgtacagc
    42601 gcctgcggga agccaccaaa gtcatgaatc gtgggcggcg tctccatcgc ggtcactcct
    42661 gttccacggg taaaccagtg agccgaaacc accacaatcg cttgcgggcg tggcaatgtc
    42721 atccccaact tctgccagct gcgggtatac aaattatctt ccagcacgtt catcggacta
    42781 ccgtgaccta aaaacaatgc tggcatacgt gttgaagaca tgatgatatc cttaactaaa
    42841 ggtgtcattt tgatatcctc acaatacgct tgttcggcgg agtaagaacc cggataacaa
    42901 tgatgatgat catcagttat tttgacgatc tgcctgaagg tgaagattta taaggagttg
    42961 tcgatgtcag tacctctcat tctgaccata ctggcggggg cagccacgtt tattggcgcg
    43021 tttctcggcg ttctcgggca aaaaccctcg aaccgcttac tggcgttttc gctaggattt
    43081 gcggcgggga tcatgttgct catctcatta atggaaatgc ttcctgccgc actagcagct
    43141 gaaggaatgt cgcctgtgtt gggttatgga atgtttatct tcggtctgct tggctatttt
    43201 ggcctggacc gcatgttgcc acatgcgcat ccgcaggatt taatgcaaaa atcggtgcag
    43261 ccgttgccaa aatcgatcaa gcgcacagcc attctgctca ctctcggcat cagtctgcat
    43321 aacttcccgg aagggattgc cacctttgtc acggcgagca gcaacctgga gctgggattt
    43381 ggcatcgcac tggccgtcgc gttgcacaat atccctgaag gtctggcagt ggcaggcccg
    43441 gtttatgcgg caacggggtc taaacgcacc gcgattctgt gggcggggat ttctggacta
    43501 gcagaaattc ttggtggtgt gctggcgtgg ttaatcctcg gtagcatgat ttccccggtg
    43561 gtcatggcgg caatcatggc ggcggttgca ggaattatgg tggcgctctc ggttgatgaa
    43621 ttaatgccgc tcgccaaaga gattgaccct aataataacc ccagctatgg cgtactatgt
    43681 ggaatgtcag tgatgggatt cagtttagtg ctgctacaaa cggcgggaat tggttaaaaa
    43741 caacatcaga agttgtccag cacatggaca acttctgcca gcttcacgaa agccttattc
    43801 taccttttcg gctgtctctt ctctcgtact gtttaagtat ttgttccgcg cttcaagcag
    43861 tgcgtctctg tcctggcgaa acgcccgaca ataacgtttc aaatgcagga aataacccac
    43921 cacaaccaga gcaatcaata caatccagta ccaggcgata aacatccttt tacccttaat
    43981 atttaacaat actaaacatt tagcgcataa atttcacata tcctttttcg gatatattca
    44041 tatggtcgca taataacgga acaattatat gattttgatc ataatgaaaa agaatgcctg
    44101 atattaatga aattttctca tagaaagaga agcggaatta acatcttgca tagcagggca
    44161 aaataaaaac cgaagccagc tacaacttcg gtttcttgat taaggcagta attaagcagc
    44221 ggttttcagc tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc
    44281 acgagcgcca tattgtgttt attggcaaac tcaatgcact ctggtgcacg cgccatcgtg
    44341 ccatcgtcat tagtcagctc acacagcaca ccagccggtt taaagcctgc cagcgtcatc
    44401 aaatcaatag tcgcttcagt atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga
    44461 agtgggaaaa cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct
    44521 gcgcgaacgg tcgtaatacg atcagcggca gaaacaccgg tagtcacacc ttcagccgct
    44581 tcaatggtca cggtaaaacc ggtgccatag gcgctggtgt tattttctac catcattggc
    44641 agatcaagtt gtttacggcg atcttcagtg atgcacaggc aaacaatacc gctaccgtgg
    44701 cgaatggtca gcgccatctg ctcaacggtc atggtttctg ccgggaagat catatcgcct
    44761 tcgttttcac ggtcttcatc atcaagcacc attacaccgc gtccttcacg cagcgcagcc
    44821 agtgcatttt caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg
    44881 gtaaaaaaac ctcactaaaa ttatggttac cagaatcagg gcagtcttag gagtggcggc
    44941 atatagccaa aataacgtga gcgggtccat gcccgacaga atcgttactc tctcccatcc
    45001 ggactctaac cgtcggcccc ggaattacac cggatctgct gtcctttgag ttcgcaccca
    45061 aagcgctcgc gggctttcaa ctgagttgat ttaccgccgg tggggaattt cgccccgccc
    45121 tgagaataag cggattcact ataacgctaa tgattagcgg cagcaacgca tagcttcaca
    45181 taattctggt ttatgactta cccttatcgc actacaatgg tactcaacac ctatcagtac
    45241 agggaaacta caatgattga cccgaaaaaa attgagcaaa tcgctcgcca ggttcacgaa
    45301 tcaatgccta aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc
    45361 ctacaagctc agctgacgcg cctcgacctt gtaagccgtg aagagttcga cgtccaaacg
    45421 caagtcctgt tacggactcg tgagaaactg gcgcggcttg aacaaagaat gagcgaactg
    45481 gaaaatcgca gtactgagat taaaaagcag ccagatccag agactctccc cccaacgctg
    45541 taaacatttg tctttaatgc actgtaaatt atgaattaat cttctaattt acagtgcttt
    45601 ctatt
//
LOCUS       DAZPJR010000013        42522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPJR010000013 DAZPJR010000000
VERSION     DAZPJR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.014"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1241
                     /locus_tag="ACHVB7_000715"
     CDS             <1..1241
                     /locus_tag="ACHVB7_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1912796.1"
                     /translation="ITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLSIIPENFF
                     TLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGDT
                     DGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYNLLNTRDM
                     ILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDGNKDSRIN
                     KNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVLTSHHGII
                     RNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRSNAVASTP
                     VYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKI
                     APNERGDKKGVPGLSCFQPQ"
     gene            1503..3125
                     /locus_tag="ACHVB7_000716"
     CDS             1503..3125
                     /locus_tag="ACHVB7_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1912797.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            3315..3428
                     /gene="yhiY"
                     /locus_tag="ACHVB7_000717"
     CDS             3315..3428
                     /gene="yhiY"
                     /locus_tag="ACHVB7_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR1912798.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            3491..4558
                     /locus_tag="ACHVB7_000718"
     CDS             3491..4558
                     /locus_tag="ACHVB7_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1912799.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            4555..7290
                     /gene="rbbA"
                     /locus_tag="ACHVB7_000719"
     CDS             4555..7290
                     /gene="rbbA"
                     /locus_tag="ACHVB7_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR1912800.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            7290..8414
                     /locus_tag="ACHVB7_000720"
     CDS             7290..8414
                     /locus_tag="ACHVB7_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1912801.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            8487..8760
                     /locus_tag="ACHVB7_000721"
                     /pseudo
     CDS             8487..8760
                     /locus_tag="ACHVB7_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            8757..9116
                     /locus_tag="ACHVB7_000722"
     CDS             8757..9116
                     /locus_tag="ACHVB7_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1912802.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(9236..9637)
                     /gene="nikR"
                     /locus_tag="ACHVB7_000723"
     CDS             complement(9236..9637)
                     /gene="nikR"
                     /locus_tag="ACHVB7_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR1912803.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(9643..10449)
                     /gene="nikE"
                     /locus_tag="ACHVB7_000724"
     CDS             complement(9643..10449)
                     /gene="nikE"
                     /locus_tag="ACHVB7_000724"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR1912804.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(10446..11210)
                     /gene="nikD"
                     /locus_tag="ACHVB7_000725"
     CDS             complement(10446..11210)
                     /gene="nikD"
                     /locus_tag="ACHVB7_000725"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR1912805.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(11210..12043)
                     /gene="nikC"
                     /locus_tag="ACHVB7_000726"
     CDS             complement(11210..12043)
                     /gene="nikC"
                     /locus_tag="ACHVB7_000726"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR1912806.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(12040..12984)
                     /gene="nikB"
                     /locus_tag="ACHVB7_000727"
     CDS             complement(12040..12984)
                     /gene="nikB"
                     /locus_tag="ACHVB7_000727"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR1912807.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12984..14558)
                     /gene="nikA"
                     /locus_tag="ACHVB7_000728"
     CDS             complement(12984..14558)
                     /gene="nikA"
                     /locus_tag="ACHVB7_000728"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1912808.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(14669..15256)
                     /gene="acpT"
                     /locus_tag="ACHVB7_000729"
     CDS             complement(14669..15256)
                     /gene="acpT"
                     /locus_tag="ACHVB7_000729"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR1912809.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(15311..16360)
                     /locus_tag="ACHVB7_000730"
     CDS             complement(15311..16360)
                     /locus_tag="ACHVB7_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1912810.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            16492..17709
                     /locus_tag="ACHVB7_000731"
     CDS             16492..17709
                     /locus_tag="ACHVB7_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1912811.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(17713..18270)
                     /gene="dcrB"
                     /locus_tag="ACHVB7_000732"
     CDS             complement(17713..18270)
                     /gene="dcrB"
                     /locus_tag="ACHVB7_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR1912812.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(18343..19008)
                     /locus_tag="ACHVB7_000733"
     CDS             complement(18343..19008)
                     /locus_tag="ACHVB7_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR1912813.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            19229..19474
                     /gene="tusA"
                     /locus_tag="ACHVB7_000734"
     CDS             19229..19474
                     /gene="tusA"
                     /locus_tag="ACHVB7_000734"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR1912814.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(19576..21774)
                     /gene="zntA"
                     /locus_tag="ACHVB7_000735"
     CDS             complement(19576..21774)
                     /gene="zntA"
                     /locus_tag="ACHVB7_000735"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR1912815.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(21848..22474)
                     /locus_tag="ACHVB7_000736"
     CDS             complement(21848..22474)
                     /locus_tag="ACHVB7_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR1912816.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            22615..22974
                     /locus_tag="ACHVB7_000737"
     CDS             22615..22974
                     /locus_tag="ACHVB7_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR1912817.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(22977..23246)
                     /locus_tag="ACHVB7_000738"
     CDS             complement(22977..23246)
                     /locus_tag="ACHVB7_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR1912818.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(23236..23832)
                     /gene="rsmD"
                     /locus_tag="ACHVB7_000739"
     CDS             complement(23236..23832)
                     /gene="rsmD"
                     /locus_tag="ACHVB7_000739"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR1912819.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            23982..25478
                     /gene="ftsY"
                     /locus_tag="ACHVB7_000740"
     CDS             23982..25478
                     /gene="ftsY"
                     /locus_tag="ACHVB7_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR1912820.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            25481..26149
                     /gene="ftsE"
                     /locus_tag="ACHVB7_000741"
     CDS             25481..26149
                     /gene="ftsE"
                     /locus_tag="ACHVB7_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR1912821.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            26142..27200
                     /gene="ftsX"
                     /locus_tag="ACHVB7_000742"
     CDS             26142..27200
                     /gene="ftsX"
                     /locus_tag="ACHVB7_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR1912822.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            27445..28299
                     /gene="rpoH"
                     /locus_tag="ACHVB7_000743"
     CDS             27445..28299
                     /gene="rpoH"
                     /locus_tag="ACHVB7_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR1912823.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            28570..29673
                     /gene="livJ"
                     /locus_tag="ACHVB7_000744"
     CDS             28570..29673
                     /gene="livJ"
                     /locus_tag="ACHVB7_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR1912824.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(29872..30255)
                     /gene="panM"
                     /locus_tag="ACHVB7_000745"
     CDS             complement(29872..30255)
                     /gene="panM"
                     /locus_tag="ACHVB7_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR1912825.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            30679..31788
                     /gene="livK"
                     /locus_tag="ACHVB7_000746"
     CDS             30679..31788
                     /gene="livK"
                     /locus_tag="ACHVB7_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR1912826.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            31836..32762
                     /gene="livH"
                     /locus_tag="ACHVB7_000747"
     CDS             31836..32762
                     /gene="livH"
                     /locus_tag="ACHVB7_000747"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR1912827.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            32759..34036
                     /gene="livM"
                     /locus_tag="ACHVB7_000748"
     CDS             32759..34036
                     /gene="livM"
                     /locus_tag="ACHVB7_000748"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR1912828.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            34033..34800
                     /gene="livG"
                     /locus_tag="ACHVB7_000749"
     CDS             34033..34800
                     /gene="livG"
                     /locus_tag="ACHVB7_000749"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR1912829.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            34802..35515
                     /gene="livF"
                     /locus_tag="ACHVB7_000750"
     CDS             34802..35515
                     /gene="livF"
                     /locus_tag="ACHVB7_000750"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR1912830.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            35799..36863
                     /gene="ugpB"
                     /locus_tag="ACHVB7_000751"
                     /pseudo
     CDS             35799..36863
                     /gene="ugpB"
                     /locus_tag="ACHVB7_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            36961..37848
                     /gene="ugpA"
                     /locus_tag="ACHVB7_000752"
     CDS             36961..37848
                     /gene="ugpA"
                     /locus_tag="ACHVB7_000752"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR1912831.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            37845..38690
                     /gene="ugpE"
                     /locus_tag="ACHVB7_000753"
     CDS             37845..38690
                     /gene="ugpE"
                     /locus_tag="ACHVB7_000753"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR1912832.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            38692..39762
                     /gene="ugpC"
                     /locus_tag="ACHVB7_000754"
     CDS             38692..39762
                     /gene="ugpC"
                     /locus_tag="ACHVB7_000754"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR1912833.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            39759..40502
                     /gene="ugpQ"
                     /locus_tag="ACHVB7_000755"
     CDS             39759..40502
                     /gene="ugpQ"
                     /locus_tag="ACHVB7_000755"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1912834.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(40489..40929)
                     /locus_tag="ACHVB7_000756"
     CDS             complement(40489..40929)
                     /locus_tag="ACHVB7_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR1912835.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            41049..>42522
                     /gene="ggt"
                     /locus_tag="ACHVB7_000757"
     CDS             41049..>42522
                     /gene="ggt"
                     /locus_tag="ACHVB7_000757"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR1912836.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        10109 a        10766 c        11694 g         9953 t
ORIGIN      
        1 ccattacagt taagattgag tgtaaggatg gttattattt tattaatttc tccggagata
       61 aatcagacat aatttataaa tcagacataa cttataaagt taatgggctt tctatcatac
      121 ctgaaaattt ctttactctt cttagcggta attttaaagc tgactggcgt tgggatgtct
      181 ctaaagagac gtttcctaag gataagtttg atagctatgt taaatctgtg ttttctaaaa
      241 tagactttta taaacaatgt ggtgtgatta acccacaaaa tgccaacacg gcttattttg
      301 gcgatacaga tggaagagta ggcgctgtac tttatgcatt gctcgtttca gggcatatag
      361 gcatcaggga aaagggttgg agtttattat gtgaattatt aaaacacgaa aacatggctt
      421 catctgcata catacataaa aataataaat tgctctacaa tttattaaat acgagagaca
      481 tgatattgaa tgagttgcat caacatgttt ttttaaagga cgatgcgata accccttgta
      541 tcttccttgg ggatcataca ggcgatagat ttagtactat ttttggtgat aaatatatcc
      601 ttactttgct aaattctatg cgaaatatgg atggtaataa agatagccgt attaataaaa
      661 atgtcatcgt tctggctgga aatcatgaaa tcaattttaa tggtaactat actgcacgac
      721 tagaaaatta taaactctct gccggtgata cgtataactt aattaaaacg ctcgatgttt
      781 gtcattatga ctcagaaacg aaagttttaa ccagtcatca cggaataata agaaatgaag
      841 aaaatgaatg ttattgtctc ggtgctttgc aggttccatt taatcagatg aaagacccaa
      901 ttgatcctga ggaattagca aatatattta ataaaaaaca taaacaacac atggatgatc
      961 gtcttatcca tctaattaga agcaatgctg tagcatccac acctgtatat gaaaattatt
     1021 ttaataacac tactgctttc agaccaaaac ctgaagatat cttcaaatgt ggacagacgc
     1081 tcaaaaaaac taaacagaaa tatggccatt atggattagg ggtagatcag catcaaaaaa
     1141 ttgataatta taccatgggg ctaaattcct ggaagatagc acctaacgaa cgtggtgata
     1201 aaaaaggtgt cccagggctt agttgttttc aacctcaata aaacttcatg agtgctgtgg
     1261 tatctctttt taattttgcc agtatttcat aggttttctt aatcgattgt agaggcatgt
     1321 tctgatgacg cctctacaat ctttagggac tttctctgct atgagataag aaatttcata
     1381 ttatgcttta aggctgaatg atttgtgcaa aatataatta ttttcccatc gaacatgagg
     1441 tgtatgtcat aagattaaat gttaactatg tattcctcgc ggtaaataat atgaggtaaa
     1501 agatgaaaat tggaactgtg gcaggcacta acgacagtac aacgacaatc acaacaaatg
     1561 atatggtgca agagcatgtt accaacttca ctaaagaact gtttgggtat attgcaaatg
     1621 gtataggtga tgatatatca agtattgcca ggacaatgct cggtgaggtt gtggaaaaaa
     1681 tagatgattg gcaaatagaa cggttccaac aatctattca agatgacaaa atttcattta
     1741 ccatacaaac gaatcattca gaaaaatata gtatgctatc gggcatgcgc gctcatattc
     1801 tacgtaggaa taactgttat cagtttattg tgacaattaa tagtaaaaat tacggttgcc
     1861 ctcttgataa taccgatatt aattggtgca gcattgttta tttacttaat aatatgacag
     1921 taaatgataa tgctaatgat gttgcagtta ctgagtcgta caaacccatc tggaactgga
     1981 aaatatcgca atacaatgta tttgatatta aattcgaaac aatgataaaa ccccagttcg
     2041 ctgacagaac ctatttctcc aattgttcac ctgttgatcc cacaagtacc agaccgacct
     2101 attttggcga taccgatggc tcagtcggtg cagttttgta cgcgcttttt gcaacgggac
     2161 atttgggaat catggctgaa ggagaaaatt tccttagcca gttgcttaat atagaagatg
     2221 aagttctcaa cgtattgcta agggagaact ttaatgagca acttgacact aatgttaata
     2281 taataatcag tatcctgaac agaagagaca ttgttttaga aagtcttcag ccttatttgg
     2341 ttattaataa agacgcagtc acgccatgta cgtttctggg cgaccagact ggcgatcgat
     2401 ttagtaatat ctgtggcgat aaatttatca tcgatttatt aaaacgcata atgagtatca
     2461 atgaaaacgt tcatgtatta gctggaaatc atgaaacgaa ttgcaatggt aactatatgc
     2521 agaacttcac gcgcatgaaa ccacttgatg aggataccta ctctgggata aaagattatc
     2581 ctgtatgttt ctatgacccc aaatacaaaa taatggcaaa tcatcatggg ataacttttg
     2641 atgatcagcg aaaacgttac attataggac cgataacagt ctctagtgat gaaatgacca
     2701 atgctctcga tccggtcgaa ttagcggcaa ttatcaataa aaagcaccat gctgtaatta
     2761 atggcaaaaa gtttaaaaat agccgggcta tctcttgtcg atcctttaat cgttattttt
     2821 ctgtttcgac agattacaga cctaaacttg aagcactatt agcatgctca caaatgttag
     2881 gtataaatca agttgttgca cataacggta atggtggaag agaatgtata ggtgaaacgg
     2941 ggacagtttt aggtcttaac gcccgtgaca gtaaacatgc tggaagaatg ttcagcatgc
     3001 ataattgcca aattaaccct ggtgcagggc ctgagataac cactccctgg aagtcttacc
     3061 agcatgaaaa aaacagaaat ggacttatgc ctttgatcag aaggcgcaca atgttacagc
     3121 tctgaaccat agacaacatg aatattaatg ttactatctg gttgagactg ttgataaaac
     3181 gtaaaaagga atgctctgtt cctgcaactg aacaggcgtg aaacgctatc caacaggatg
     3241 gataccgttt tgtcagccag ttttatagat cacacttatg agtatgcgtg tttcatttac
     3301 ggccaggaat gctgatgatg acaacattac tacccgtttt cactaagcct tccccgttgg
     3361 cgctcaatgc tctgcgcgct ggtcgtattt gtcgtttcct tcttatcccg gatggaagaa
     3421 tccgctgaac gtcgttcggc ggcagttgta acacgcgtcg tttgcttgca gggattatgg
     3481 cgggtcgatt atggataaga gtaagcgcca tctggcgtgg tgggttgtcg ggttactggt
     3541 ggtggctgcc gtcgtggcgt ggtggctgtt gcgcccggcg ggtgtgccgg aaggctttgc
     3601 tgtcagcaat gggcgcattg aagcgacgga agtggatatc gccagcaaaa ttgccgggcg
     3661 tatcgacacc attctggtga aagaaggcca gtttgttcgc gaaggcgaag tgctggcgaa
     3721 gatggatact cgcgtgttgc aggagcagcg actggaagcc atcgcgcaaa tcaaagaagc
     3781 acaaagtgcc gttgctgccg cgcaggcttt gctggagcaa cggcaaagcg aaactcgtgc
     3841 cgcgcagtcg ctggttaatc aacgccagtc cgaactggac tccgtggcta aacgtcatac
     3901 gcgttcccgc tcgctcgccc aacgaggggc tatttctgcg caacagctgg atgacgatcg
     3961 cgccgccgct gagagcgccc gagctgcgct ggaatcggca aaagctcagg tatcggcttc
     4021 taaagcggct atagaagcgg cccgtaccaa tatcattcag gcccaaactc gcgtcgaagc
     4081 cgcgcaagcc actgaacggc gtattgccgc agatatcgat gacagtgaac tgaaagcccc
     4141 gcgtgacgga cgcgtgcagt atcgggttgc tgagccaggg gaagtgctgg cggcaggcgg
     4201 tcgggtgctg aatatggtcg atctcagcga cgtctatatg actttcttcc tgccaaccga
     4261 acaggcgggc acgctgaaac ttggcggtga agcccggctg atcctcgatg ccgcgccaga
     4321 tctgcgtatt ccggcgacca tcagttttgt cgccagtgtc gcccagttca cgccaaaaac
     4381 cgtcgaaacc agcgatgaac ggctgaaact gatgttccgc gtcaaagcgc gtatcccacc
     4441 ggaattactc cagcagcatc tggaatatgt caaaaccggt ttaccgggcg tagcgtgggt
     4501 gcgggtgaat gaagaacttt cgtggcctga cgacctcgtg gtgaggttgc cgcaatgacg
     4561 catctggaac tggttcccgt cccgcctgtc gcgcaactgg cgggcgtgag ccagcattat
     4621 ggaaaaaacg ttgcgctgaa caatattact ctcgatattc cggcccgctg tatggtcggg
     4681 ctgattggtc cggatggcgt cgggaagtcg agcttattgt cgttgatttc cggtgcccgc
     4741 gtcattgagc agggcaatgt gatggtgctg ggcggcgata tgcgcgaccc gaggcatcgc
     4801 cgcgacgtct gcccgcgcat cgcctggatg ccgcaggggc tgggcaaaaa cctctaccac
     4861 accttgtcgg tgtatgaaaa cgtcgatttt ttcgctcgcc tgttcggtca cgacaaagcg
     4921 gagcgggaag tgcgaattaa tgagctgctg accagcacgg ggttagcacc gtttcgcgat
     4981 cgtccggcag ggaaactctc cggcgggatg aagcaaaaac ttgggctgtg ctgcgcgtta
     5041 atccacgacc cggaactgtt gatccttgat gagccaacaa cgggggttga cccgctctcc
     5101 cgctcccagt tctgggatct gatcgacagt attcgccagc ggcagagcaa tatgagcgtg
     5161 ctggtcgcca ccgcctatat ggaagaggcc gaacgcttcg actggctggt agcgatgaat
     5221 gccggagaag tgctggcaac tggcagcgcc gaagagctac ggcagcaaac gcaaagcgct
     5281 acgctggaag aagcatttat aaatctgtta ccgcaagcgc aacgccaggc gcatcaggcg
     5341 gtagtgatcc caccgtatca acctgaaaac gcagagattg ccatcgaagc gcgcgatctg
     5401 accatgcgtt ttggttcctt cgttgccgtt gatcacgtta atttccgcat tccacgcggg
     5461 gagatttttg gttttcttgg ttcgaacggc tgcggtaaat ccaccaccat gaaaatgctc
     5521 accggactgc tgcccgccag cgaaggtgag gcgtggctgt tcgggcaacc ggttgatcca
     5581 aaagatatcg atacccgccg tcgggtgggc tatatgtcgc aggcgttttc gctctataac
     5641 gaactcaccg tgcggcaaaa ccttgagtta catgcccgtt tgtttcacat cccggaagcg
     5701 gaaattcccg caagagtggc tgaaatgagc gagcgtttta agctcaacga cgttgaagat
     5761 gttctgccgg agtcattgcc gctcggcatt cgccagcggc tttcgctggc ggtggcggtg
     5821 attcatcgcc cggagatgtt aatcctcgat gagcctactt ctggtgtcga tccggtggcg
     5881 agggatatgt tctggcagtt gatggtcgat ctctcgcgcc aggacaaagt gactatcttc
     5941 atctctaccc actttatgaa cgaagcggaa cgttgcgacc gcatctcact gatgcacgcc
     6001 ggaaaagtgc ttgccagcgg tacaccgcag gaactggttg agaaacgcgg agccgccagt
     6061 ctggaagagg catttatcgc ctatttgcag gaagcggcag ggcagagcaa cgaagccgaa
     6121 gcgccgcccg tggtacacga caccacccac gcgccgcgtc agggatttag cctgcgccgt
     6181 ctgtttagct acagccgccg cgaagcgctg gaactgcgac gcgatccagt acgttcgacg
     6241 ctggcgctga tgggaacggt gatcctgatg ctgataatgg gttacggcat cagtatggat
     6301 gtggaaaacc tgcgctttgc ggtgctcgac cgcgaccaga ccgtcagtag ccaggcgtgg
     6361 acactcaacc tctccggttc ccgttacttt atcgaacagc cgccgctcac tagttatgac
     6421 gagcttgatc gtcggatgcg tgcgggcgat atcacggtgg cgattgagat cccgcccaat
     6481 ttcgggcgcg atatcgcgcg tggtacgcct gtggaactcg gcgtctggat cgacggagcg
     6541 atgcccagtc gtgccgaaac ggtaaaaggt tacgtgcagg caatgcacca gagttggctt
     6601 caggatgtgg cgagccgaca atcgacaccc gccagccaaa gcgggctgat gaacattgag
     6661 acgcgctatc gctataaccc ggacgtaaaa agcctgccag cgattgttcc ggcggtgatc
     6721 ccgcttctgc tgatgatgat cccgtcaatg ctaagcgccc ttagcgtggt gcgggaaaaa
     6781 gagctagggt cgattatcaa cctttacgtg acccccacca cccgtagcga atttttgctt
     6841 ggcaaacagc tgccgtacat cgcgctgggg atgctgaact ttttcctgct ctgcggcctg
     6901 tcggtgtttg tgtttggcgt accgcataaa ggcagtttcc tgacgctcac cctggcggcg
     6961 ctgctgtata tcatcattgc caccggaatg gggctgctga tctccacctt tatgaaaagc
     7021 cagattgccg ccattttcgg aacggcgatt atcacgttga tcccggcgac acagttttcc
     7081 gggatgatcg atccggtagc ttcgctggaa gggcctggac gttggatcgg cgaggtttac
     7141 ccgaccagtc attttctgac tatcgcccgc ggaacgttct cgaaagcgct ggatctgact
     7201 gatttgtggc aactttttat cccgttgctg atagccatcc cgctggtgat gggcttaagc
     7261 atcctgctgc tgaaaaaaca ggagggatga tgcgccattt acgcaatatt tttaatctgg
     7321 gtatcaaaga gttgcgcagt ctgctcggtg ataaagcgat gctgacgctg attgtcttct
     7381 cgtttacggt gtcggtgtat tcgtcagcga ccgttacgcc aggatcgttg aacctcgcgc
     7441 cgatcgccat tgccgatatg gatcaatcgc agttatcgaa ccggatcgtt aacagcttct
     7501 atcgtccgtg gtttttgcca ccggagatga tcaccgccga tgagatggat gccggactgg
     7561 acgccggacg ctataccttc gcgataaata ttccgcctaa ttttcagcgt gatgtcctcg
     7621 ccggacgcca gccggatatt caggtgaacg tcgatgccac gcgcatgagc caggcattta
     7681 ccggcaatgg gtatatccag aatattatca acggtgaagt gaacagcttt gtcgcgcgct
     7741 accgtgataa cagcgaaccg ttggtatcgc tggaaacccg gatgcgcttt aacccgaacc
     7801 tcgatcccgc gtggtttggc ggggtgatgg cgatcatcaa caacattacc atgctggcga
     7861 ttgtattgac cggatcggcg ctgatccgcg agcgtgaaca cggcacggtg gaacacttac
     7921 tggtgatgcc gataacgccg tttgagatca tgatggcgaa gatctggtcg atggggctgg
     7981 tggtgctggt ggtatcggga ttatcgctgg tgctgatggc gaaaggtgta ctgggcgtac
     8041 cgattgaagg ctcgatcccg ctgtttatgc tgggcgtggc gctcagtctg tttgccacca
     8101 cgtcaatcgg catttttatg gggacgatag cgcgttcaat gccgcaactg gggctgctgg
     8161 tgattctggt gctgctgccg ctgcaaatgc tttccggtgg atccacgccg cgcgaaagta
     8221 tgccgcagat ggtgcaggac attatgctga ccatgccgac gacacacttt gttagcctcg
     8281 cgcaggccat tctctaccgg ggtgccggat tcgaaatcgt ctggccgcag ttcctgacgc
     8341 tgatggcaat tggcggcgcg tttttcacca ttgcgctgct gcgattcagg aagacgattg
     8401 ggacaatggc gtaaggaagg gaaacttgac ttcatactgt gatgtcatac tattgatgtc
     8461 ataaagtgaa gtcataagga catgccatga gaaccaaggt acaggcttta cggaagaaaa
     8521 aaaaatacat tggatcaaat atttaaaact cctgttcctc aagggatcaa gtggtctgat
     8581 atagagtcac tggttaaagc attaggcgga gaaattaagg aaggaagagg ttcgcgttgt
     8641 aagttcatac taaatatgag cgttgcgtgt ttccatcggc ctcatccgtc gccagatacc
     8701 gataaaggcg ctgtagaaag cgtgcgtgac tggttactaa gtataggggt aaaaccatga
     8761 tcaaactgaa gacgccaaac tctatggaga ttgccgggca accagctgtt attacctacg
     8821 tgccagaact gaatgcattt cgtggcaaat tcctgggctt gtcaggttat tgtgattttg
     8881 tctctgacag cattcagggg ctgcaaaaag aaggggaact ttccctgcga gagtatcttg
     8941 aggattgtaa agctgcgggt attgagccgt atgcgcgtac agaaaagatt aagaccttta
     9001 cgttacgcta tccagaatcg ctgagtgaac gccttaataa tgcggctgcg cagcaacaag
     9061 tctctgttaa tacctatatt attgaaacac tgaatgagcg tttgaatcat ttataaattt
     9121 tagcggcgta cattacgcag aatatctgcc tttacaggat ctatctatgc aactgaactt
     9181 aattttataa attaagttca gttaatatct tttctttcct cgtttcagaa ataactcaat
     9241 cttccttcgg caagcactgc aaatgcccat gccgcacgcc gcgctgggcg ataacgtcat
     9301 cggcaaaatg ctgcacgtcg cccatatcac ctttcagcac ggcgatttcc agacagtcgt
     9361 cgtgattgat atgcacatgc agcgtggcga cggagagatc gtggtgatgg tgctgggtgg
     9421 agacaatgcg gctggctaag tcgcgttttt cgtgttcata cacataggag agcaccgcga
     9481 aaccttgcgt gccgtgctgc tgagtggcct cctgggccag ggcgctacgc agaatatcgc
     9541 ggatagcttc ggaacggttg ttataaccac gacgctggct caggctgtcc agcgtctcca
     9601 gtaaatcgtc atcaagcgtg atggtgactc gttgcatttg cgttaaacct tttctgtggt
     9661 gcgacggcgc acggggaatg cgggtaatac cgcgttttgt agcacacgtc cggcgtcaga
     9721 ggaaaaggtt aatttatctc ccaccacctg ggtttcgacg atttgtccgt tgtccatcac
     9781 catcacccgc tgacagaagc gttccaccag gcgtaagtcg tgggtgatga acaggcaggc
     9841 ggtgccaaac tgttgttgta gctttttcag caggcgaatg acgcccgcct gtaacacgag
     9901 atcaaggtta gaaacggctt catccagaat cagtagtttc ggttcgaccg ccagcgcgcg
     9961 agccaggcag acgcgctgga gctggccgcc gcttaactgc ggtgggcgtt tgtcgagaac
    10021 gctgtcatcg agatcgaccg ccttcagcat ttcgctggcg cgcgccagtt gttcggattt
    10081 tttcagtgat agcaggtggc gcatcggttc acgcaggatc tcgcgcacgg ttttgcgcgg
    10141 attcacggcg ctgatggagt cctgaaatac catctgaata tcgcggcgga acgctttacg
    10201 ctgggcgcga ttgagtttcg ccagcggttc gccacgccag ctaatattcc cctgcgcggg
    10261 cgattctaaa cccaccagca accgcgcgag ggtacttttc ccgcagccgc tgcgccccag
    10321 cagggcgacg gtttcgccgc ttttcagggt cagggaaacg ttattcagca ccgcctgatg
    10381 ttgatgtttt ccgttaaatc caccgtgggc atagtgatgg gaaaggccgg agacgttaag
    10441 taaagtcatg atgccagctc cataccgtag agggcgagat gagcggaaac caggctgcgc
    10501 gttaccgcat gtttgggggc gttaaacagc gtttctacat cgccctgttc gacaatttta
    10561 ccttgtgaca tcaccgccac gtcatccgcc agacgcgcca caacgcccat atcatgggtg
    10621 accagcagca ttcccggcgc ttgtttttgc ataatgcttt ccagcagatc gaggatgcgc
    10681 gcctgtgcta ccacgtcgag gtcggtggtc ggttcatcgg cgatgataaa cggtgattca
    10741 cacagcaccg ccatcgcaat catcatgcgc tgcaacatgc cgccgctcat ctcgaacggg
    10801 tacagcttca gcacgcgcgc ggcgttttcc agccccaccg cttctatggc agcggtaagc
    10861 gtagcgtcat cggcgggttt ccctaacgcc aggcaggttt cccgcgcgtg ggtgtgcatg
    10921 gtgtgtagcg gattaaaggc gctgcgcggg ttctgcatga tggtggcaat tttgatgccg
    10981 cgcagggcgc agggataaac cgctttgcca tcggctaaaa tttcccccgc cgtctggcga
    11041 acgccagcgg gcaaaatgcc cagcgtcgcg gcgcaggtta atgatttccc gctgccgcta
    11101 ccgccgacta acgccagcac gcgcccgcgt tgcagggtta acgatacacc gtgtaccagc
    11161 ggctgcgcgg cctgtagcgc gatattacgt agttcaatct gttgcggcat tagtgtgcgt
    11221 gctccgtcac cagatgagga tccagatgat cgcgcagtgc gtcacccacc aggttaaagg
    11281 ccatcacgct gataaacagc gccagccccg gccagaacat ttgcagcggc tgggtccaga
    11341 tatactggcg cgcgtcgtta atcatcacgc cccattcggc ggtcggcgcg gtcacaccga
    11401 ggccaaggaa agacatcccc gcgacgtgca gcatcatatg gccgatatcc agcgttgcca
    11461 gcaccagcag cgaagggatc accgcgcctg ccagatgatc gacaaacacc cgcacatggc
    11521 ccgcaccgga aagccgtgac gccagcacaa actcgcgttg gcgtagtgaa atcaccaagc
    11581 tgcgcaccat gcgtgcatac cacgcccagt gcgacagggc gatggcgata attacgttgg
    11641 tcagcccggt gccgagcacg ccaaccataa agaacgacag aatcgaggtc gggaaggtca
    11701 taaacatatc ggcgacgcgc atggtggcct gatcaacgcg cccgccaatc aacccggcgc
    11761 tgccgccaat aaccagccct aatgtcagca ccagcagcag gcaggccatt accgaaccga
    11821 gcgacacgcg ggtcgctgcc atcagccgcg agaaaatatc gcgacctaag tgatcggtgc
    11881 ccagccagtg ctgcgcatcc ggcgaaagca ggcgcgacgg caaatcaatc gcctgggggt
    11941 catacggcag ccaccactgg ctggtgagcg caatcagcgc cagcagggcg ataatgatca
    12001 gcgccaggcg taccgaccag cgggaagaga ggaaaaagtt cacgcgtgcg ctccttcatg
    12061 acgacgaatg cgcgggtcca gcgcggcgtt gagcaaatcg acaatcaaat tacagaccac
    12121 aaaaaccacc accatcatca gcgtaaagca ctgaatcacc ggatagtcac ggttaaaaat
    12181 cgccgacacc gcatagcgcc cgacgcccgg ccaggcaaag atgttttcga taatcatcgt
    12241 cccgccaatc agttcgccga tgtgcatccc cacggcggtg atcatcggca gcgaggcatt
    12301 gcgcagaata tggcggcgtt cggtctgttt gtcgttcagg ccgcgcagac gcgcccaagt
    12361 gacgtgacgc tgaccggcga cgtccagcat actggcgcgc agcagtcgcg cgttaatcgc
    12421 cagcgacata aaggcaatgg aaaccgcagg caaaatgatg tgctgccagc cgccgtaacc
    12481 catcgcgggt agccattgca gatacaccga aaacgccatc accagtaaaa acgccagcca
    12541 gaagttaggc atcgacacgc caagaaacgc gatgaaacgt acggcgaaat ccggcagacg
    12601 gtcgcgatgg cgcgccgccc agataccgag cggtacggaa gtgagcagaa ttaataccag
    12661 cgccgcacct gcaagttcca gcgtggcggg caggaagttc agcatatcgt ccagcaccgg
    12721 gcgttggctg gcgaatgaga taccaaagtc aagatgcagc gccttccaca accaggtacc
    12781 gtactggacg tacagcggct gatccagccc gagcatggtg cgggtagagg ccagcatctc
    12841 cggcgtcggc ggcaggttag ataaacgcag ataatcgagc gccgggtcgc cggtaccgag
    12901 gcgcagcatc agaaaaatga tcaccgaggc ggcgagcacc atcgggatca gcagcagaaa
    12961 gcggcgtaat acgtaacgca acattaaggt ttcaccggtt taatctgttc gaacggaatt
    13021 tcggtggcga tcggcgcgta ggggatatta cccagctccg gttttgatac caccatcatc
    13081 gagatgtagc tgataggcag ataaaccgcc tcgtcatgca gacgggtcaa aatgtcgcga
    13141 tacagcgcct gacgttgcgt ttcgtcatgg gtcgccagca cttcgccgat ctctttatca
    13201 atcagcggct tgtcggctaa tccttgctgt gcctggaagt cagcgtgcga cggtacgcgc
    13261 attgaactca ggaaggcgtg tggatcgtat ggcgcgcccc aggtgcggtg gaaaatcatg
    13321 ccaaaacgac cgtcgcgctg acgagcatag atactgctct cttcttcgcc aatcagcgag
    13381 acatctgcgc caatctggcg catatcagcc tgaatgattt ccgccatcga tttgcttaac
    13441 gcatcggtgc cgatgaacga cagttcaatg cgcagcggct gaccattttt ctcgcggatg
    13501 tctttgcccg caggcagcgt ccaaccggct ttttccagca actctttcgc tttctgcgga
    13561 tcgtactggc gcggtttcag gccgagatta gcgtaaggca cagaaggggc aaacagggtg
    13621 tcggcgacct gcggggtgcc atataacgcg ttatcaatca gcgatttttt gtttaccgcg
    13681 taattaagag cttcacgtac tgccagctcg ttggtggggg ctttggcggt attgagcgcc
    13741 agcatcacgg tttcgatcgg ctgtgacagt tgggtgtggt aagccggatt ctggctaaag
    13801 cgggcgaagg tatcgagcgg taataaccct tcgtttccgt acagcaggtc gatatcgcca
    13861 gtttcaaacg ccaccgcgcg ggtagtcggg tccgggatga cgttaaaggt gatcttttta
    13921 atcgctggct tttcgcccca gtagttttcg ttacggacga agacatcgta ctgattcagt
    13981 ttcgattcct gcaaaatcca cggtccggtg ccaatcggcg ctttaattcc gttcatggtt
    14041 tcatggtttt taaactgcga gggagcgata aaacggaaag gacggggcag ggccagttct
    14101 tgcaggaaag gatagtaggc gcttttcagg gtaatttgca gctctgtttt actgagtgct
    14161 ttaacatcaa caatctggtt tgccagctcc agccaggcgt gacgttgacg gttatcgagc
    14221 actgcgcgga agttttctgc cgccgcctcg gcatcgaacg gttcaccgtt ggagaatttc
    14281 acgtcatcac gcagggtgaa ggtccaggtt ttaccatctt ctgaatgagt ccagcttttt
    14341 gccagccacg ggatcaccga accgtctgcc tgatatttca ccaatggttc ataaaccatg
    14401 ctctgggcga acatctggtt aggcgtgtaa aggtgcgggt ttagtggccc gacattcacc
    14461 ggccaggcgg tagtgatttc atctggtgca gcggcatgga cgataaaaga cgcacaagcc
    14521 agcagcgcaa atagagtgcg gcggagtgtg gagagcatgg taaccccaat ggattaaaat
    14581 agatggcgga aataagtatg acgattttaa gtattcgtca tactgattac ctgttctgga
    14641 tcaatagatg ggcagtcggg tgggcggatc agttaactga atcgatccat tgcacactgt
    14701 cggcggtgag cgtaaagggg gtgggggtgc agatcgcaag gcttaaattt tctagctgac
    14761 aatggctgac tgacagcgag gagtgatagg tgctatctac gctgacgatt tgccaggcgc
    14821 tgccgccacg ctgtttaacg atggcttctt tgcgcgtcca gatgcgccag aacatttcca
    14881 gttgctgatc aggatgcacg gcgtccattt cagcgtgttc cccgaggctg aacacggcgt
    14941 tcgccagcca gcgccagttg gcgcgcgggc gaatcacttc gatatcgcag ccgacttcgc
    15001 cttcatcgct caacagcaga gcgatatcgt caccgctatg gcttaagttg aaccatagcg
    15061 gcgtttccgg cgcgaatgca ggtttgcctt gttcgccata gatgatctcc ggtagcgggg
    15121 aaagcgtgtg cgaaagcaat gcacgccccg ccagccagcg ttcgcgtcgt ggaccttgcg
    15181 gtgcttgctc gcgtaaaccc ggtggcagtg gagctgcgct taaggtcgaa actttcccca
    15241 gaactatccg atacatatca gggccaacgt ttaatggaaa atgaaagtgc gtatcgtatc
    15301 acttgtcgcc tcatcccggt aaccgacttt tcggtctgcc tggccccagt aaaatcgcca
    15361 gtttgctacc gcctttggtg gtttccatcc agattttaca cacactggtt aatggcaccg
    15421 aaagtagcat ccctaccggg ccgagcagcc atccccaaat taacaatgaa agaaatacca
    15481 ccatggtgga catccccagg cgatggccca tcatccgtgg ttctaaaata ttgccgatga
    15541 ccatatggac cactaaaaac aatgcgccga ccagaataca ttcgtaaaca ccattaaaca
    15601 gcagcacctg aatcattggc ggtacggcgg aaattaccgc gccgatattg ggcacgtagt
    15661 tgagcaaaaa cgccagtact gcccacatca gcgcaaactg cacccccatc agctccagcc
    15721 ccagccagac gatgacacct gtccataaac tgagtagcgt cttcaatgca agatagtgcg
    15781 aaacgccttt aagtgcgcgg tgtaatcctg cgatgtgaat ctgtgggtta ttcagcgcaa
    15841 aacgcatttt gtaagggacg tggcgcactt caaacagcat aaaaactacg gtcatcacca
    15901 gcaaaagcac gctcgccatt gccccggaaa gcccggtcat tagcgctgtg gtgaaggtaa
    15961 ccactttttc cgagtccatc cgctgcagca ttcgctccgg cgacatatgc aaattaagaa
    16021 aaggcaacat ctcctgcaat ttaaaaagtt tgcgcgtcag ctccttatta aacttcggca
    16081 gcatagagat aaattcgtta aacgatgccg ccagtacgcc gaccagcgcg gttagtgcga
    16141 tcagcatcac caccactaca atcgtaatgg caacggggcg ttgtactccc cgacgaataa
    16201 accaggtaac gagcgggttg aggacgatgg caaaaaacag cgccagtaac agctgcacaa
    16261 tgatatctgc cgctgcgtga atgcccgcga ggatcactac cagcgaggcc agcttgagca
    16321 gaatgtgcat gcccgtttta tcgggttgag ggatttccat tggggcttcc ttgtgacttt
    16381 ttgtattaag tgtagcggga gtcacgccag caatattctg attcttactg ctgattttca
    16441 cgtcagcacg tggtaaaaat gaaacactgt tgtaaaaatg tggtgatcct catgcccgaa
    16501 cccgtagccg aacccgcgct aaacggattg cgcctgaatt tgcgcattgt ctccattgtc
    16561 atgtttaact tcgccagcta cctcaccatc gggttgccgc tcgctgtatt accgggctat
    16621 gtccatgatg tgatgggctt tagcgctttc tgggcaggat tggttatcag cctgcaatat
    16681 ttcgccacct tgctgagccg tcctcatgcc ggacgttacg ccgatttgct gggccccaaa
    16741 aagattgtcg tcttcggttt atgcggctgc tttttgagcg gtctgggata tctgacggca
    16801 ggattaaccg ccagtctgcc cgtcatcagc ctgttattac tttgcctggg acgcgtgatc
    16861 cttgggattg ggcaaagttt tgccggaacg ggatcgaccc tgtggggcgt tggcgtggtt
    16921 ggctcgctgc atatcgggcg agtgatttcg tggaacggca ttgtcaatta cggggcgatg
    16981 gcgatgggtg cgccgttagg cgtcgtgttt tatcactggg gcggcttgca ggcgttagcg
    17041 ttaatcatta tgggcgtggc gctggtggcc attttgttgg cgatcccgcg tccgacggta
    17101 aaagccagta aaggcaaacc actgccgttt cgcgcggtgc ttgggcgcgt ctggctgtac
    17161 ggtatggcac tggcactggc ttctgccgga tttggcgtca tcgccacctt tatcacgctg
    17221 ttttatgacg ctaaaggttg ggacggtgcg gctttcgcgc tgacgctgtt tagctgtgcg
    17281 tttgtcggta cgcgtttgtt attccctaac ggcattaacc gtatcggcgg cttaaacgtg
    17341 gcgatgattt gctttagcgt tgagataatc ggcctgctac tggttggcgt ggcgactatg
    17401 ccgtggatgg cgaaagtcgg cgtcttactg gcgggggcag ggttttcgct ggtgttcccg
    17461 gcattgggcg tagtggcggt aaaagcggtt ccgcagcaaa atcagggggc ggcgctggca
    17521 acttacaccg tatttatgga tttatcgctt ggcgtgaccg gaccactggc tgggctggtg
    17581 atgagctggg cgggcgtacc ggtgatttat ctggcggcgg cgggactggt cgcaatcgcg
    17641 ttattactga cgtggcgatt aaaaaaacgg cctccggaac acgtccctga ggccgcctca
    17701 tcatcttaaa acttactgaa taaccagcgt attaatgatg ttttctgcgg tggtctgcgc
    17761 tttttgctga tcgtcagctg gcagagtaat ttgcatggtc agcagttgat tacccacgtt
    17821 acccagaata acggaagagt acgccgtctg gcctttcgcg gagataatac tgtctaactg
    17881 ctgcattttg tgacctttca gctcaatggc tttgttggtt accacttgca gctgcggatc
    17941 gcggctacgt tgctgatctt ccagacgctt cgccaacacc gccagatctt ctttcggatc
    18001 atcgcccatg atgacgatga ctgctttctg cccggtggcg tcggaccaga catgcatgtt
    18061 attggcctgc gttcccagct taccgctctg gtcggtcata tccgctggca gcgagaaact
    18121 taacttgcca tcaagcaggt tgacgggatt cccggtagcg ttactttccg cgaccgaacc
    18181 ctgcgccgta gcgttagtgt ctttatcatc acaggccgca agccccataa ccagcaggcc
    18241 aattccgaca tatttaacca gattgcgcat tgacttcttc ctttcgataa acggccataa
    18301 cggctcattc atccatctta tcacaactct gataacgaac ctttaactcg cctgcaaagc
    18361 gttgatttcg gatttatctg ccagtctttt caacagaata ttgagtaata cgccatacat
    18421 tggcaggaag aaaacgatac tgattaacac tttgaaacag taatcgacca gcgcgatttc
    18481 catccagtgt tcagccataa aggcatccgg gctacgccag aaggcaatga agaaaaaggc
    18541 cagcgtgtcg ctgacgttac cgaacagtgt ggacgctgtc ggtgccagcc accagcggcg
    18601 actctgacgc aggcggttaa aaacgtgcac gtcgaggatc tgtcccagcg cgtaggccat
    18661 gaaactggcg gtggcgatac gggcgacaaa caggttgaag tgggcgagtg cgccgaatcc
    18721 ctgccaggaa cccatataga atagcgacga gatgacgtag gagatcagca acgccgggat
    18781 catcaccgca aagatgatac gacgggccag cggtgcgcca aaaatacgca cggtcaggtc
    18841 ggtagcaagg aaaataaacg ggaagctaaa cgcgccccag gtggtatgga aacccaaaat
    18901 ggagacggga agctgaacca gatagttact ggaggtgatc accagcagat gaaataacga
    18961 taaccagaac aacgccttat agcgttgagt ttgcgagaaa acgttcatat tgtacctttt
    19021 tgattaacca ttggggtgag ggaacccaat acgtacgaca cgtctgttac cttaacataa
    19081 taacgtgctt accctttttt cgagccgccg catgatactg ctttacgttg acaatgcaat
    19141 ggttaatttt cacgcaatcg ttaacctggt ttgcttacgg actcacaggg cgtaaactag
    19201 cgccgttttt ttatgtgatg agaagaaaat gaccgatctc ttttccagcc ctgaccacac
    19261 actcgacgca cttggcctgc gctgcccgga acccgtaatg atggtgcgca aaactgtgcg
    19321 caatatgcag cctggcgaaa cgttgctgat tatcgccgac gatccggcca ctacccgcga
    19381 tattcctggg ttttgtacct ttatggaaca cgaactggtt gcgaaagaga cggatggact
    19441 gccttatcgt tatttgattc gtaaaggcgg ttgatgggga aagattggct tcgatgccgc
    19501 cttttcccct caccctaacc ctctccccag aggggcgagg ggaccgatcg cgctcaatgt
    19561 tgcgatcggt ttgccttatc tcctgcgcaa caatcttaac gcattcgctg tcaccagcac
    19621 cgtcgcaccc gtatctgcca gcaccgccag ccacaggccg gtcatcccta acagcgtggt
    19681 gacgaggaag atccctttca gccccagcgc aatggtgatg ttctggcgga tattggcgtg
    19741 agtggcgcgt gccagttcaa tcatttgcac caggccgcgc aggtggttat gggttaatgc
    19801 tgcatcagcg gtttccagcg ccacgtctgt gccactgccc attgcaatcc caatggcggc
    19861 agctttcatc gctggcgcgt cgttaatacc atcaccgacc atcgccaggg gcgcatgttg
    19921 attcagcttg gtcaccgctt tgactttatc ttccggcaat aggcccgctt taaactccag
    19981 ccccagctcc ccggcgattg ccgccgctgc acgtggatta tcgccggtga ggatcactcc
    20041 tttgaccccc agcgcgttca gttcactgat ggcagttgca gcatcggcgc gcagggtatc
    20101 ctgcaatgca atgataccca gcacgtcatc attacgtact accagcacaa ccgtttgccc
    20161 ggcgctttcc agttcattaa tcaaaccagc aaatgcatca gcgggatgtt tcccggcagc
    20221 gcaaatcaat acgcgctcac cgttaacctg cgcttcaatg ccagacccga ccagcgcccg
    20281 ctgtgattcg gcggtgggaa tggcgagttc agcaacctgt gcttcgcgta cgatggcttg
    20341 cgccagtgga tgcgtcgcgc cttgctcgac cgccgccgcc agtgtcagca gttcagattc
    20401 actaataccc gttgccggat gaatcgcggt aacgcgcggt ttaccgacgg tcagcgtacc
    20461 ggttttatca aacgccacct gagtaacacg acctagttgt tccagcgccg ctccgccttt
    20521 aatcaacgcc ccacgacgcg ctgccgccgc cagcccggag gtaatcgccg caggcgttga
    20581 gataactaac gcacacgggc agccaatcag cagcagcgtc agccctttat aaatccactc
    20641 ctgccagctg gcggcaaaca gcagcggtgg caccagcgtc accagcagag cgacggccat
    20701 aatcgcgggc gtatagatac ggctgaaacg gtcgataaac cgctcaatgg gggcgcgacg
    20761 ctcttcggct tcttcgatca gtttcagaat ccggtcaatg gcgctggcac ccggttctga
    20821 cagcacttcc agcgttacca gacggtctac gctggtggct cctgcaggaa ctttatcgcc
    20881 cgtcgcgcgc tccaccggaa tggattctcc ggtcagggcg ctttcatcaa aactggcaaa
    20941 cggtgagagc agtttaccgt cggcaggcaa acgcccacct gcggcgactt caatcacatc
    21001 gccaggccgc aggctgttaa tcgccacctc ttcccgctca ccgttacgca ggcgcgtggc
    21061 ggtttctggt ttcagcgcca ttaacgcgct aacgccctga cgcgcgcggc tggcggccca
    21121 gccttccagt cgttcaccaa tcaaaaacag cagcaacacc atcgcagctt cagccgttgc
    21181 gccaataaac agtgcaccaa tagcggctac gctcattaag gtttcaatgg cgaagtagct
    21241 gccggatttg atcagccgta atgcctgacg agcaatcggg tacagcccaa ccagcgtggt
    21301 cgcgataaac gccagttgcc cgaacggatg attgaactgc tccagacccc agctgattgc
    21361 catcatcacg attagtgtaa tcagcggcag attctctttc aggcgcgatg cttgtggctc
    21421 gtcggcggcc tgctcatcgc gcagggaata gcccgctttt tgcaccgcag attcaacttg
    21481 tgcacgaatg tcattgtcgg catcaaccac cagtttttcg gtggcgaaca acacctgcac
    21541 ctgattcaca cctgcaagct ggcgcacggc attttctacc ttgcgcgcac aggcggcgca
    21601 gtccatgcca ctgactttcc agctatagcg ggtgccggag acgttttcag agagcgttgg
    21661 cgagctggag catgcgccgt cgcagcaaca gtcgttggcg ttctgtaccg tggttagcgg
    21721 tttgaacgca gcaaattgag gggctttctt gccgtgattg tcaggtgtcg acatggcata
    21781 ctccggttaa gttttttctc attaaccgaa ggatacactc tggagtcgac tccagagtca
    21841 agttttatca gagatacagt gagcggatga tgaggaagtg accggcaaag tagcaggccg
    21901 cagcaatcgc gttatccgca cggaagcgtc ggcgatagtg gctccccagc cagacaaagt
    21961 tactgataaa cagcaacgaa gcgccaacaa acgcagagag cgccggagcg gtcggacgga
    22021 agaaccacag ttcacctgcc agccacacca tcaccagcgt catgccgata aacgtgcaga
    22081 taggccaacg atactcttcc agccgcgtcc agataatcgc cagtaacagc gcacccagaa
    22141 ccagcaacac cagtggcaga ggccagaaga aagagagcgt catctgactg gcgaaataga
    22201 tggtgtacag caggtgcgag aggaaaaacg cgccgatggc gtacatcaga cgttgacgtg
    22261 gcaacagggt tagcgcatcg cccagcagtg aggcgcacag ccctgccagc accagatagc
    22321 taatggcgtc gaacatcggc gcttgccagg ccagtaacag caggagaaga agggttaacg
    22381 gtttaaacac ccagcgttgc caggttggcc cacgatacga tgcatccaca gatagccatg
    22441 cggaaagaca gacagcgata aacgaccaaa gcatcttagt tccttgaatt cgttattttt
    22501 tcgtaagcgg aatgcctacg gtcttgcttc cagtttagtg gcgagggtgg acggatgaca
    22561 acaccataac tggcagggta tgcttatttc cgcattttcc gatgagggat aaagatgagc
    22621 aaaccacctc ttttctttat tgttatcatt ggcttaattg tcgtcgccgc atcgtttcgt
    22681 tttatgcaac agcgacggga aaaagctgat aatgatatgg ctccgctcca gcaaaagctg
    22741 gtggtggtga gcaacaagcg ggaaaaaccg attaacgatc gccgttcgcg ccagcaggaa
    22801 gtgactccgg caggcaccag tatgcgctac gaggcaagct tcaaaccgca aagcggagga
    22861 atggagcaga cgtttcgcct cgacgcccag cagtaccacg ccctgacagt gggcgataaa
    22921 ggtacgctga gctataaagg aacgcgcttt gttagctttg ttggcgaaca ataaggttat
    22981 tttttaactt taaatttctt ctgccagacc agcagttcaa acacgccgaa aaggaaaatc
    23041 cgtacctttt cggcggtggt catctgcggg ccatctttcg gtaaagtgga tttcaatagc
    23101 gccagctgca taccatgcat cagcacggta aaaatcagcg ccacgttaac gaaaatattt
    23161 agcgggcgcg ggaagggatg caccagattg aggattaaaa atccccaaac gcagagcatt
    23221 aacaaacgac caatattaat cagcatcact ttctccttgt gcttcgcgtt gatacagccg
    23281 ataagccacc tgacccgcca ctttttcccg atgtaacgac cagtttgccg gaacggtggg
    23341 cagaccgttt tcgacttcgc tttcgacata aatcagggct tcgtcagcca gccagccgtt
    23401 atcttccagt aaatttatcg tctcttctaa caagccacgg cggaacggtg gatcgacaaa
    23461 cacgatatta tgcggtgtgc ctttttgcgc caggaatgac atcgcgttgc tgttcaccac
    23521 gcgtgcattg cctgctttta gtgtcgccag attcttaatt aactgctgag aaactgcacg
    23581 atccatctca attaacgtgg cccccgcagc gtagcgcgat aacgcttcca gccccagcgc
    23641 gccgctcccg gcgaagcaat ccagacattg ggcgtcaaca atgaccggag ccagccagtt
    23701 aaacaacgtt tcgcgtacgc ggtcggtggt ggggcgcaga cctgggctat caggaaccgg
    23761 gagtttacgg cctcgccact gcccgccaat aatgcggatt tggccgctgc cggaatgatt
    23821 cggttttttc atgataaatt gctcaatccg ccagataaca catacttgca ggcggtgatg
    23881 tgaattaagt taagtgatag actatttcat cattttttta gctgctatgt acatagcgtt
    23941 aacgctgtgc catgaagcaa cagcgaggag tgtagtcgca aatggcgaaa gaaaaaaaac
    24001 gtggcttttt ttcctggctg ggctttggtc aaaaagagca gaccccggaa aaagagacag
    24061 aagttcagaa tgaacaaccg gttgtagaag aaatcgttca ggcgcaagag cctgtgaagg
    24121 cttcggaaca agccgttgaa gagcagccgc aggcgcatac tgaagccgag gcggaaactt
    24181 ttgctgccga cgttgtggaa gtcactgaac aggttgttga aagtgaaaaa gcgcagcctg
    24241 aagcggaagt tgttgcacag ccggaaccgg tcgtagaaga aacgccggag ccagtggcta
    24301 tcgaacgtga agagctgccg ttgccggaag acgtcaacgc cgaagaggtt tcgccagaag
    24361 agtggcaggc tgaagcggaa accgtagaga ttgtcaaagc ggcggaagaa gaagcggcta
    24421 aagaagaaat caccgacgaa gagctggaag cacaggcgct ggcagccgaa gcagcagaag
    24481 aggcggtgat ggtggttcct ccggcagaag aagagcagcc ggtggaagaa atcgctcagg
    24541 agcaggaaaa accaaccaaa gaaggtttct tcgcgcgcct gaaacgcagc ctgttaaaaa
    24601 ccaaagaaaa tctcggttcc ggatttatca gcctgttccg cggtaaaaaa atcgacgatg
    24661 atctgtttga ggagctggaa gagcagcttt tgatcgccga tgtgggcgtg gaaaccacac
    24721 gtaaaattat caccaatctg acggaaggcg catcccgcaa gcagcttcgt gatgccgagg
    24781 cgctctatgg cctgctgaaa gaagagatgg gtgagattct ggcgaaagtc gatgagccgc
    24841 tgaatgttga aggtaaaacg ccgttcgtga tcctgatggt gggcgtcaac ggtgtgggta
    24901 aaaccacgac gattggtaag ctggcacgtc agtttgagca gcagggtaaa tcggtgatgc
    24961 tggcggcggg cgatactttc cgtgcagcag cggttgaaca gcttcaggtc tggggtcagc
    25021 gcaacaatat tccggtgatt gcccagcata ccggggcgga ttccgcctct gttatcttcg
    25081 acgccattca ggcagctaaa gcgcgtaata tcgacgtcct gattgccgat accgccggac
    25141 gcctgcagaa caaatcgcac ctgatggaag agttgaagaa aatcgtccgc gtgatgaaga
    25201 aactcgacgt tgaagcgccg catgaagtta tgctgactat tgatgccagc accgggcaga
    25261 acgcggtaag ccaggccaaa ctgttccacg aagctgttgg cttaaccggc atcacgctaa
    25321 cgaaactgga cggcacggcg aaaggcgggg taattttctc ggtggctgac cagtttggta
    25381 tccctatccg ctacattggt gtcggcgaac gtattgagga tttgcgtccg tttaaggcgg
    25441 acgactttat agaggcactt tttgcccgag aggattaaca atgattcgct ttgaacatgt
    25501 cagcaaggct tatctcggtg ggagacaggc gctgcagggc gttacgttcc atatgcagcc
    25561 gggtgagatg gcgtttctga ccggtcattc cggcgcaggg aaaagtaccc tcctgaagct
    25621 gatctgtggg attgagcggc ccagcgccgg gaaaatctgg tttagcggcc atgacatcac
    25681 gcgtctgaaa aaccgtgaag ttccgtttct gcgccgccag attggcatga ttttccagga
    25741 tcaccatcta ctgatggacc gtactgtcta cgataacgtg gcgatcccgc tgattatcgc
    25801 tggtgccagc ggtgacgata ttcgtcgccg ggtgtcggcg gcactggata aagtcgggct
    25861 actggacaaa gcgaagaact tccctattca gctttccggc ggtgaacaac agcgtgttgg
    25921 cattgcccgc gcggtggtga ataagcccgc ggtactgctg gcggacgaac cgaccggtaa
    25981 cctggacgac gcgttgtcgg aagggattct acgtctgttt gaagagttta accgcgttgg
    26041 ggtaaccgta ttgatggcaa cgcacgacat caacctgatt tcacggcgtt cccatcgcat
    26101 gctcaccctg agcgatggtc acttgcatgg aggcgtgggc catgaataag cgcgatgcaa
    26161 tcaatcatat tcggcagttt ggtgggcgtc tggatcgctt ccgtaaatcg gtcggcggct
    26221 caggcgacgg cggtcgtaac gcaccgaaac gcgcgaaatc ctcgccaaaa ccggtaaatc
    26281 gcaaaaccaa cgttttcaac gaacaggtgc gctatgcctt ccacggcgca ttgcaggatc
    26341 tgaaaagcaa accgttcgcc acgtttttaa cggtgatggt tatcgccatc tccctgacgc
    26401 tgcccagcgt ctgttatatg gtgtacaaaa acgttaatca ggcggcgacg cagtattatc
    26461 cgtcaccgca aatcactgtt tacttgcaaa aaacgctgga cgatgacgcc gctgcgggcg
    26521 tggtggcaca gttgcaggcc gagcaaggcg tggagaaagt gaactatctt tctcgtgaag
    26581 acgcactggg cgagttccgt aactggtctg gttttggtgg tgcgctggat atgctggaag
    26641 aaaacccgct tccggcagtg gcggtggtga tcccgaaact cgattttcag gggacggaat
    26701 cactcaatac gctgcgtgat cgtatcacgc agattaacgg cattgacgaa gtgcggatgg
    26761 atgacagctg gtttgcccgt ctggcggcgt tgaccgggct ggtcgggcgc gtttcggcga
    26821 tgatcggcgt gttgatggtg gcggcagtgt tcctcgtcat cggcaacagc gtacgtctga
    26881 gcatctttgc tcgccgtgac tccatcaacg tgcagaaact gattggtgcg acggatggtt
    26941 tcatccttcg cccgttcctg tatggtggcg cactgctggg attttctggc gcattgttgt
    27001 cattaatttt gtcagaaatt ctggtgctgc gattgtcatc ggcggttgcg gaagtggcac
    27061 aggttttcgg aacgaagttt gatatcaatg atttatcgtt cgatgaatgc ctgctattgc
    27121 tgctggtatg ctcgatgatt ggctgggtgg cagcgtggct tgccacggta caacatttac
    27181 gccactttac gcctgaataa taaaagcgtg ttatactctt tccctgcaat gggttccgta
    27241 gcagggaaag agatcccgtt gtctcttccc ggtatttcat ctctatgtca cattttgtgc
    27301 gtaatttatt cacaagcttg cattgaactt gtggataaaa tcactgtctg ataaaacagt
    27361 ggatgataac ctcgttgctc ttaagctctg gcacagttgt tgctaccact gaagcgccag
    27421 aagatatcga ttgagaggat ttgaatgact gacaaaatgc aaagtttagc tttagcccca
    27481 gttggcaacc tggattccta catccgggca gctaacgcgt ggccgatgtt gtcggctgac
    27541 gaggagcggg cgctggctga aaagctgcat taccatggcg atctggaagc agctaaaacg
    27601 ctgatcctgt ctcacctgcg gtttgttgtt catattgctc gtaattatgc gggctatggc
    27661 ctgccacagg cggatttgat tcaggaaggt aacatcggcc tgatgaaagc agtgcgccgt
    27721 ttcaacccgg aagtgggtgt gcgcctggtc tccttcgccg ttcactggat caaagcagag
    27781 atccacgaat acgttctgcg taactggcgt atcgtcaaag ttgcgaccac caaagcgcag
    27841 cgcaaactgt tcttcaacct gcgtaaaacc aagcagcgtc tgggctggtt taaccaggat
    27901 gaagtcgaaa tggtggcccg tgaactgggc gtaaccagca aagacgttcg tgagatggaa
    27961 tcacgtatgg cggcacagga catgaccttt gacctgtctt ccgacgacga ttccgacagc
    28021 cagccgatgg caccggtgct ctatctgcag gataaatcat ctaactttgc cgacggcatc
    28081 gaagatgata actgggaaga gcaggcggca aaccgtctga ccgacgcgat gcaaggtctg
    28141 gacgaacgca gccaggacat catccgcgcg cgctggctgg acgaagacaa caagtccacg
    28201 ttgcaggaac tggctgaccg ttacggtgtt tccgctgaac gtgtacgcca gctggaaaag
    28261 aacgcgatga aaaaactgcg cgctgctata gaagcgtaat ttccgctatt aagcagagaa
    28321 ccctggatgc gagtccgggg tttttgtttt ttgggcctct acaataatca atttcccctc
    28381 cggcaaaacg ccaatcccca cgcagattgt taataaactg tcaaaatagc tattccaata
    28441 tcataaaaat cgggtatgtt ttagcagagt atgctgctaa agcacgggta gccatgcata
    28501 aaacgaaata aagtgctgaa caacaacatc acaacacacg taataaccag aagaatgggg
    28561 attctcagga tgaacataaa gggtaaagcg ttactggcag gatgtatcgc gctggcattc
    28621 agcaatatgg ctctggcaga agatattaaa gtcgcggtcg tgggcgcaat gtccggtccg
    28681 gttgcgcagt acggtgacca agagtttacc ggcgcagagc aggcggttgc ggatatcaac
    28741 gctaaaggcg gcattaaagg caacaaactg caaatcgtaa aatatgacga tgcctgtgac
    28801 ccgaaacagg cggttgcggt ggcgaacaaa gtcgttaacg acggcattaa atatgtgatt
    28861 ggtcacctct gttcttcatc aacgcagcct gcgtctgaca tctacgaaga cgaaggcatt
    28921 ttaatgatca ccccagcggc aaccgcgccg gagctgaccg cccgtggcta tcagctgatc
    28981 ctgcgcacca ccggcctgga ctccgaccag gggccgacgg cggcgaaata tattcttgag
    29041 aaagtgaaac cgcagcgtat tgctatcgtt cacgacaaac agcaatacgg cgaaggtctg
    29101 gcacgagcgg tgcaggacgg cctgaagaaa ggcaatgcta acgtggtgtt ctttgatggt
    29161 atcaccgccg gggaaaaaga tttctctacg ctggtggcgc gtctgaagaa agagaatatc
    29221 gacttcgttt actacggcgg ttaccacccg gaaatggggc aaatcctgcg tcaggcacgc
    29281 gcggcagggc tgaaaactca gtttatgggg ccggaaggtg tggctaacgt ttcgctgtct
    29341 aacattgcgg gcgaatcagc ggaagggctg ctggtgacca agccgaagaa ctacgatcag
    29401 gttccggcaa acaaacccat tgttgacgcg atcaaagcga aaaaacagga cccaagcggc
    29461 gcattcgttt ggaccaccta cgccgcgctg caatctttgc aggcgggcct gaatcagtct
    29521 gacgatccgg ctgaaatcgc caaatacctg aaagcgaact ccgtggatac cgtaatggga
    29581 ccgctgacgt gggatgagaa aggcgatctg aaaggctttg agttcggcgt atttgactgg
    29641 cacgccaacg gcacggcgac agatgcgaag taatcattaa tcggcaactt tgggttgccg
    29701 ccaaattgct aatatcgagt acgttgcttc atgccggatg cggcgtaaac gccttatccg
    29761 gcctataaga tccaaagaaa tcagtaaatt gcaacacaca ttgtaggcct gataagcgta
    29821 gcgcatcagg caatttctcg ttggtcatca cttttgaaat caacttgacg attaacgctt
    29881 ctcccagccg ccctgttgcg ccgtaaatcc cagtgcctgc ataaacgctg tcatcacacc
    29941 gcgatcttcc acgcctgcat ccgccatcca ccagcatgaa acgccaggat tgttacgcaa
    30001 aacctcttcc agcagatatt gccccacacc gcgacgtcgg gtgacttccc gcacacgcag
    30061 ggaatccagt gctccctcgg tgccgcttaa ggttacccgt acggcagcga gcaggcgctc
    30121 gttaaaacgc gcggcgtaga tacggtggtt atcgtcaacc tgtaaagagg aaggggaata
    30181 ctccggccag atcttttgca ggtcaatccg gtcctggtcg ctaaagtttt ctaatcgaat
    30241 gatggtcagc ttcatgggta acccgtgtaa atcacaaaag tggaaccagt gtagcgaaat
    30301 aatttaatcg gaggctttct cttttttatt tcttttggca ggtgattaat tttttaacag
    30361 caataattac aaagttaaaa cattagagaa tgaaaaatac ccagcataat cccctgaatg
    30421 gtagtgaatt attccgccct ttgtgccgtt attttatgct gacaaaggca cttttttctg
    30481 tttatctatc aataaattca gaatattatc tgttcttaat cgactgaaaa atagggattt
    30541 taatcactgt tatcacaaaa tactgcgcta aacccttaat cagacaggca aaaacagtgc
    30601 agtataaaaa aagaacagtc tgatttgtta acacataaaa acaaagcaac acaacatcac
    30661 gaatggggat ttttgactat gaaacggaat gcgaaaacta tcatcgcagg gatgattgca
    30721 ctggcaattt cacacaccgc tatggctgac gatattaaag tcgccgttgt cggcgcgatg
    30781 tccggcccga ttgcccagtg gggcgatatg gaatttaacg gcgcgcgtca ggcgattaaa
    30841 gacattaatg ccaaaggggg aattaagggc gacaagctgg ttggcgtgga atatgacgac
    30901 gcctgcgacc cgaaacaagc cgttgcggtc gccaacaaaa tcgttaatga cggcattaaa
    30961 tacgttattg gtcatctgtg ttcttcttct acccaacctg catcagatat ctacgaagac
    31021 gaaggtattc tgatgatctc gccgggagcg accaacccgg agctgaccca acgcggttat
    31081 caacacatta tgcgtactgc cgggctggac tcttcccagg ggccaacggc ggcaaaatac
    31141 attcttgaga cggtgaagcc ccagcgcatc gccatcattc acgacaaaca acagtatggc
    31201 gaagggctgg cgcgttcggt gcaggacggg ctgaaagcgg ctaacgccaa cgtcgtcttc
    31261 ttcgacggta ttaccgccgg ggagaaagat ttctccgcgc tgatcgcccg cctgaaaaaa
    31321 gaaaacatcg acttcgttta ctacggcggt tactacccgg aaatggggca gatgctgcgc
    31381 caggcccgtt ccgttggcct gaaaactcag tttatggggc cggaaggtgt gggtaacgca
    31441 tcattgtcga atattgctgg cgatgctgcc gaaggcatgt tggtcactat gccaaaacgc
    31501 tatgaccagg atccggcaaa ccagggcatc gttgatgcgc tgaaagcaga caagaaagat
    31561 ccgtccgggc cttatgtctg gatcacctac gcggcggtgc aatctctggc gactgccctt
    31621 gagcgtactg gcagcgatga gccgctggcg ctggtgaaag atttaaaagc taacggtgca
    31681 aacaccgtga ttgggccgct gaactgggat gaaaaaggcg atcttaaggg atttgatttt
    31741 ggtgtcttcc agtggcacgc cgatggttca tccacggcag ccaagtgatc atcccaccgc
    31801 ccgtgaaatg cgggcgggtt tagaaaggtt accttatgtc tgagcagttt ttatatttct
    31861 tgcagcagat gtttaacggc gtcacgctgg gcagtaccta cgcgctgata gccatcggct
    31921 acaccatggt ttacggcatt atcggcatga tcaacttcgc ccacggcgag gtttacatga
    31981 ttggtagcta tgtctccttt atgatcatcg ccgcactgat gatgatgggc attgataccg
    32041 gctggctgct ggtggcggcg ggattcgtcg gcgcaatcgt cattgccagc gcctacggct
    32101 ggagtatcga acgggtagcc tatcgcccgg tgcgtaactc taagcgcctg attgcgctca
    32161 tctcggcaat cggcatgtcc atcttcctgc aaaactacgt tagcctgacc gaaggttcgc
    32221 gtgacgtggc gctgcccagt ctgtttaacg gtcagtgggt ggtggggcat agcgaaaact
    32281 tctctgcctc tattaccacc atgcaggcag tgatctggat tgttactttc ctcgccatgc
    32341 tggcgctgac gattttcatt cgttattccc gtatggggcg cgcctgccgt gcctgcgcgg
    32401 aagatctgaa aatggcgagc ctgcttggca tcaacaccga ccgggtgatt gctttgacct
    32461 ttgttattgg cgcggcgatg gcggcagtgg ctggcgtact gctcggtcag ttctacggcg
    32521 tcattaaccc ctatatcggc tttatggccg ggatgaaagc ctttaccgct gcggtgctcg
    32581 gtgggattgg cagcattcca ggggcgatga ttggcgggct gatcctgggg attgcggagg
    32641 cgctctcttc tgcctatctg agtacggaat ataaagatgt ggtctcattc gccctgctga
    32701 ttctggtgct gctggtgatg ccgaccggta ttctgggtcg cccggaggta gagaaagtat
    32761 gaaaccgatg catattgcaa tggcactgct ctctgccgcg atgttctttg tcctcgctgg
    32821 cgtctttatg ggcgtgcaac tggagctgga tggcaccaaa ctggtggtcg acagcgcttc
    32881 ggatgtccgt tggcagtggg tgtttatcgg cacggcggtg gtctttttct tccagctttt
    32941 gcgacctgct ttccagaaag ggctaaaaag cgtttccggg ccgaagttta ttctgcccgc
    33001 gattgatggc tccacggtga agcagaaact gttcctcgtg gcgctgctgg tgcttgcggt
    33061 gtcgtggccg tttatggtgt cacgtgggac ggtggatatc gccactctga ccatgatcta
    33121 cattatcctc ggtctcgggc tgaatgtggt tgttggtctt tctggtctgc tggtgctggg
    33181 gtacggcggt ttttacgcca tcggcgctta cacttttgcg ctgctcaatc actattacgg
    33241 tctggggttc tggacctgtt tgccgattgc cggactgatg gcagcggcgg cgggcttcct
    33301 gcttggtttt ccggtgttgc gtctgcgcgg tgattatctg gcgatcgtta ccctcggttt
    33361 cggcgaaatt gtgcgcatat tgctgctcaa taacaccgaa attaccggcg gcccgaacgg
    33421 tatcagtcag atcccgaaac cgactttctt cggccttgag ttcagccgta ccgcccgtga
    33481 aggcggttgg gacacgttca gtaatttctt tggcctgaaa tacgatccct ccgatcgcgt
    33541 catcttcctg tatctggtgg cgttgctgct ggtggtgcta agcctgtttg tcattaaccg
    33601 cctgctgcgg atgccgctgg ggcgtgcgtg ggaagcgttg cgtgaagatg agatcgcctg
    33661 ccgttcgctg ggcttaagcc cgcgtcgtat caagctgact gcctttacca tcagtgccgc
    33721 gtttgccggt tttgccggaa cgctgtttgc tgcgcgccag ggctttgtca gcccggaatc
    33781 cttcaccttt gctgaatcgg cgtttgtact ggcgatagtg gtgctcggcg gtatgggatc
    33841 gcagtttgcg gtgattctgg cggcaatttt gctggtggta tcgcgcgaat tgatgcgtga
    33901 tttcaacgaa tacagcatgt taatgctcgg tggtttgatg gtgctgatga tgatctggcg
    33961 tccgcagggc ttgctgccca tgacgcgccc gcaactgaag ctgaaaaacg gcgcagcgaa
    34021 aggagagcag gcatgagtca gccattatta tctgtgaacg gcctgatgat gcgcttcggc
    34081 ggcctgctgg cggtgaacaa cgtcaatctt gaactgtacc cgcaggagat cgtctcgtta
    34141 atcggcccta acggtgccgg aaaaaccacg gtttttaact gtctgaccgg attctacaaa
    34201 cccaccggcg gcaccatttt actgcgcaat cagcacctgg aaggtttacc ggggcagcaa
    34261 attgcccgca tgggcgtggt gcgcaccttc cagcatgtgc gtctgttccg tgaaatgacg
    34321 gtaattgaaa acctgctggt ggcgcagcat caacaactga aaaccgggct gttctctggc
    34381 ctgttgaaaa cgccttcttt ccgtcgcgcc cagagcgaag cgctcgaccg cgccgccacc
    34441 tggcttgagc gcattggttt gctggaacac gccaaccgtc aggcgagtaa cctggcctat
    34501 ggtgaccaac gccgtctgga gattgcccgc tgcatggtga cgcagccgga gattttaatg
    34561 ctcgacgaac cggcggcagg tcttaaccca aaagaaacca aagagctgga tgagctgatt
    34621 gccgaactgc gcaatcatca caacaccact atcttgttga ttgaacacga tatgaagctg
    34681 gtgatgggga tttcagaccg aatttatgtg gtcaatcagg ggacgccact ggcaaacggt
    34741 acgccggagc aaatccgtaa taacccggac gtgatccgtg cctatttagg tgaggcataa
    34801 gatggaaaaa gtcatgttgt cctttgacaa agtcagcgcc cactacggca aaatccaggc
    34861 gctgcatgag gtgagcctgc atatcaatca gggcgagatt gtcaccctca ttggcgcgaa
    34921 cggggcgggg aaaaccacct tgctcggcac gttatgcggc gatccgcgcg ctaccagcgg
    34981 gcgaattgtg tttgatgata aagacattac cgactggcag acggcgaaaa tcatgcgcga
    35041 agcggtagcg attgtcccgg aagggcgtcg cgtcttctcg cggatgacgg tggaagagaa
    35101 cctggcgatg ggcggctttt ttgccgaacg cgaccagttc caggagcgca taaagtgggt
    35161 gtatgaactg ttcccacgtc tgcatgagcg ccgtgttcag cgggcaggca ccatgtccgg
    35221 cggtgagcaa caaatgctgg cgattggtcg cgcgctgatg agcaacccgc gtttgctgct
    35281 acttgatgag ccatcgcttg gtcttgcgcc gattatcatc cagcaaattt tcgacaccat
    35341 cgagcagctg cgcgagcagg ggatgactat cttcctcgtc gagcagaacg ccaaccaggc
    35401 gctaaagctg gcggatcgcg gctacgtgct ggaaaacggc catgtggtgc tctccgatac
    35461 tggtgatgca ctactggcga atgaagcggt gagaagtgcg tatttaggcg ggtaataaca
    35521 cgttgattga taaggagtca aaagactcct ttgagacagg tgacaaatgt aaaattgcct
    35581 gatgcgctgc gcttatcagg cctactgggt gagttgcaat atattgaatt tgcacgatct
    35641 tgtaggccgg ataaggcgtt tacgccgcat ccggcatgaa acgatgagta atctgtagag
    35701 tttgattcag accttctatt tttccgctta tccgtgcccc atctcccatt ttccctcacc
    35761 cacgccgtca ccgccttgtc atctttctga caccttactt gcaggtttat gaagttggca
    35821 ccgccaccat gatggcatcg aaagccatta aaccggtata tgacgtgttt aaagaggcag
    35881 ggattcaatt cgatgagtcg cagtttgtgc cgacggtttc cggttactac tccgacagca
    35941 aaacgggcca cttactctcc cagccattca acagctcgac tcccgttctc tattacaaca
    36001 aagacgcctt caagaaagcg gggttagacc cggaacagcc gccgaaaacc tggcaggatc
    36061 tggcggactc ttccgcaaaa ctgaaagcct ccggtatgaa gtgcggctac gccagcggct
    36121 ggcagggctg gatccaactg gaaaacttta gcgcctggaa cggtctgccg tttgccagca
    36181 aaaacaacgg ctttgacggc acggacgcgg tgctggagtt caacaagccg gagcaggtga
    36241 aacacattgc catgctcgaa gagatgaaca agaagggcga cttcagctac gtcggtcgta
    36301 aggatgaatc caccgagaag ttctataacg gtgattgcgc gatgaccacc gcctcttccg
    36361 gttctctcgc taatattcgt gagtacgcca aatttaacta tggcgtaggc atgatgcctt
    36421 acgacgccga tgcgaaagat gcaccgcaaa acgccattat cggcggggcc agcctgtggg
    36481 tgatgcaggg caaaaataaa gaaacgtata ccggcgtggc gaagttcctc gacttcctcg
    36541 cgaagccaga aaacgctgcc gagtggcatc agaaaaccgg ttatctgcca atcaccaaag
    36601 cagcgtatga cctgacccgt gagcagggct tttatgagaa aaacccaggg gcggataccg
    36661 cgacgcgtca gatgctgaat aagccgccgt tgccgttcac caaagggctg cgtctgggca
    36721 acatgccgca gatccgcgtg attgtggatg aagagctgga gagcgtgtgg accggtaaga
    36781 agacaccaca gcaggcactg gataccgccg ttgagcgtgg aaatcagttg ctgcgccgct
    36841 ttgagaaatc gacgaagtct taatcagtgt aatgtcggat gcgtttcgct tatctgacct
    36901 ggcatcgcgt gtaggccgga taagcgaagc gcatccggca cagttcagga attaaccgta
    36961 atgtcatcat cccgtccggt gttccgctcg cgctggctgc cttatctgct ggtcgcgccg
    37021 cagctcatca tcaccgttat cttttttatc tggcctgcgg gcgaagcgtt gtggtactcg
    37081 cggcaaagcg tcgatccgtt tggtttctcc agccagtttg tcggtctgga taacttcgtc
    37141 acgctgttcc atgacagcta ctatctagac tccttctgga cgacgataaa attcagcacc
    37201 tttgtcaccg tcagcggttt gctggtatcg ctgttctttg cggcgctggt ggagtacatc
    37261 gtgcgcggca gccgtttcta tcaaaccttg atgctgctgc cgtatgccgt ggctcccgcc
    37321 gttgccgccg tattgtggat cttcctgttt aaccccggtc gcgggctgat cacccatttt
    37381 ctcgctgagt tcggctacga ctggaaccac gcgcaaaaca gcggtcaggc aatgtttctg
    37441 gtggtgtttg cctcggtgtg gaagcaaatc agctacaact ttctgttctt ctatgccgcg
    37501 ctgcactcca ttccccgttc gttgattgaa gccgcagcta ttgacggcgc agggccgatt
    37561 cgccgcttct ttaagattgc tctgccgctt atcgctccgg tgagtttctt cctgctggtg
    37621 gtgaacctgg tgtacgcctt cttcgacacc ttcccggtga tcgacgccgc cacgtccggc
    37681 gggccggttc aggccaccac tacgctgatt tataagatct accgcgaagg ctttaccgga
    37741 ctggatctgg cttcgtctgc cgcgcagtcg gtggtgttga tgttcctcgt catcgtgctg
    37801 acggtggtgc agttccgcta tgttgaaagc aaggtgcgtt accaatgatt gagaaccgtc
    37861 cgtggctgac gatattcagc cataccatgc tgatcctcgg gatcgcggtg atcctcttcc
    37921 cgctgtacgt ggcgtttgtc gcggcgacgc tggataaaca ggccgtctat gccgcgccga
    37981 tgacgctcat ccccggcaca catctgctgg aaaatatcca caacatctgg gttaacgggg
    38041 taggcacgaa tagcgcgccg ttctggcgga tgttgcttaa cagctttgtg atggcgttca
    38101 gcattacgct cggcaaaatt accgtctcga tgctctcggc atttgccatt gtctggtttc
    38161 gttttccgct acgtaacctc ttcttctgga tgatttttat caccctgatg ctgccggttg
    38221 aagtacgtat cttcccgacg gtggaagtca tcgccaacct gaaaatgctc gacagctacg
    38281 ccggtttaac gctgccgctg atggcctcgg cgaccgccac gtttctgttc cgccagttct
    38341 ttatgaccct accggatgaa ttggtggaag ccgcgcggat cgacggcgcg tcaccaatgc
    38401 gtttcttttg cgacatcgtt tttccgttgt ccaaaactaa tctggcggcg ctgtttgtga
    38461 tcacctttat ctacggctgg aatcagtatt tgtggccgtt gttgattatt accgacgttg
    38521 atctcggcac caccgtggca gggatcaaag ggatgatcgc tacaggcgaa ggcaccacgg
    38581 aatggaactc ggtaatggcg gcgatgttgt taacgcttat ccctccggtg gtgattgttt
    38641 tagtgatgca gcgtgccttc gtgcgcggcc tggtcgatag tgagaaataa gatggcagga
    38701 ctgaaattac aggcagtaac caaaagctgg gatggcaaaa cccaggtcat taaaccgctg
    38761 acccttgatg tggcggatgg cgaatttatc gtgatggtcg ggccgtctgg ctgcgggaaa
    38821 tcgacgctgc tgcgcatggt tgccgggctg gagcgggtga cagaaggcga tatctggatc
    38881 aacgaccagc gcgtgaccga aatggagccg aaagatcgcg ggattgcgat ggtgttccag
    38941 aattacgcgc tttatccgca tatgagcgta gaggagaaca tggcgtgggg gctgaaaatt
    39001 cgcggcatgg gcaagcagca aattgccgag cgcgttaaag aagcggcgcg cattctggaa
    39061 ctggacggtc tgcttaagcg tcgtccgcgt gagctatccg gcggtcagcg ccagcgtgtg
    39121 gcgatgggcc gcgcgattgt gcgcgatccg gcggtgttct tgtttgatga gccgctctct
    39181 aacctcgatg ccaagctgcg cgtgcagatg cgtcttgaac tgcaacagtt gcaccgtcgc
    39241 ctgaaaacga cttcactcta cgttactcac gatcaggttg aagcgatgac gctcgcccag
    39301 cgagtaatgg tgatgaacgg tggcgttgcc gaacagattg gcacaccagt tgaagtctac
    39361 gaaaagcccg ccagcctgtt tgtggcgagt tttatcggca gcccggcgat gaacctgctg
    39421 acaggccgcg tgaataatga aggcacgcat ttcgaactgg acggcggtat cgtgctaccg
    39481 ctaaacggtg gctaccgtca gtatgccggg cgtaaaatga ctctcggcat tcgcccggaa
    39541 catatagcgc taagctcgca ggcagaaggt ggtgtgccgc tggtgatgga cacgctggag
    39601 atcctcggcg cagataacct ggcgcacgga cgctggggcg agcagaagct ggtggtgcga
    39661 ctggcgcatc aggagcgccc gacggcaggc agcacgctgt ggctgcattt ggcggaaaat
    39721 cagctgcatc tttttgatgg tgaaacagga caacgagtat gagtaactgg ccttatcccc
    39781 gcatcgtcgc tcatcgtggc ggcggtaagc tggccccgga aaacaccctg gcggcaatcg
    39841 acgtcggggc aaaatacggt cataagatga tcgaatttga cgcgaagtta tcgaaagatg
    39901 gcgagatctt cctgctccat gacgacaatc tcgaacgcac cagcaacggc tggggcgtcg
    39961 cgggtgaact gaactggcag gatttactgc gcgtggatgc gggcagttgg tacagcaaag
    40021 cgtttaaagg tgagccgctg ccgttgcttt cgcaggtggc ggaacgctgt cgcgaacacg
    40081 ggatgatggc gaatatcgaa atcaaaccca ccactggcac tggaccatta acgggcaaaa
    40141 tggtggcgct ggcggcacgc caactgtggg ccggtatgac gccgccgctg ctgtcatcgt
    40201 ttgagattga tgctttagaa gctgcacaac aggcggcacc ggaactgccg cgtggtttat
    40261 tgcttgatga gtggcgcgac gactggcgcg aactgaccgc gcggctgggc tgcgtctcta
    40321 ttcatctcaa tcataagtta ctcgataaag cgcgagtgat gcagttgaaa gacgccggat
    40381 tacggattct ggtttatacc gtcaataaac cccagcgcgc ggcagagttg ctgcgctggg
    40441 gcgtggattg catctgtacc gatgcgattg acgtgattgg tccgaacttt acggcccaat
    40501 agttttcaac ggaatgtcag gctgcggcgt gctgggctga ttcagcatgt cgccgtttct
    40561 cctttccggc agcatatgct gctgattcaa cgaactatcc ggattacggt tgctgtttaa
    40621 catcccgccg ttggtgttgg gcagcatttg ctgccgggcg ggatttcgct cccccggctg
    40681 tgactgcaac acccgctgag aattattgtt tatctggttt tctaaatgct gctgttgcaa
    40741 ctgagtttgc gttttcagtt gctgattcag catccctttt tgctggattt gctgagtctg
    40801 catctgggtt tgcatccgct gctggctggg tatctgatac cccggctggt tagggttgtt
    40861 cagagtatta atgggctgtg caaagccgac aaacggcagg agtgccgtaa gaatcagaag
    40921 tcgtttcatc gcgtatcctc ctctgaagat atcctttaag tttactcgct tcccgacaaa
    40981 acgatgatta attcagagtt atataccagg cttagctggg gttgcccctt aatctctgga
    41041 gaataacgat gataaaaccg acgtttttac gccgggtggc cattgctgct ctgctctcag
    41101 gaagttgttt tagcgccgcc gccgcgcctc ctgcgccgcc cgtctcgtat ggtgtggagg
    41161 aagatgtctt ccacccggta cgcgcgaaac agggaatggt agcgtctgtg gacgccactg
    41221 ccactcaggt gggggtggat atcctcaagg agggcgggaa tgccgttgat gccgccgtgg
    41281 cggtgggcta cgcgctggcg gtaacgcatc cgcaggcagg gaatctgggc ggtggtggtt
    41341 ttatgttaat ccgctcgaaa aatggcaata ccacggctat cgatttccgc gaaatggcac
    41401 ccgccaaagc gacccgcgat atgttcctcg atgatcaggg caacccggac agcaaaaaat
    41461 cactcacttc gcatctggct tccggcacac cgggtacggt agcaggtttc tcgctggcgc
    41521 tggataaata cggcaccatg ccgctgaaca aagtcgtgca gcccgcgttt aaactggcac
    41581 gcgatggttt tatcgttaac gacgcgctgg ctgacgatct caaaacctac ggtagcgaag
    41641 tgttgccgaa tcacgaaaac agtaaagcta tcttctggaa agagggcgag ccgctgaaaa
    41701 agggcgacac gctggtgcag gcgaacctgg caaagagcct ggagatgatt gctgaaaacg
    41761 gcccggacga attctataaa ggcacgattg cggaacagat cgcccaggag atgcagaaaa
    41821 acggtggctt gatcactaaa gaagatttag cggcctataa agcggtcgaa cgcactccga
    41881 taagcggcga ttatcgcggg tatcaggttt actccatgcc accgccatcc tccggcggga
    41941 tccatatcgt acaaatcctc aatattctgg aaaacttcga tatgaagaaa tacggctttg
    42001 gcagcgccga tgcgatgcaa atcatggcag aagcggagaa atacgcctac gccgaccgct
    42061 cggaatatct tggcgacccg gattttgtca aagtcccgtg gcaggcactg accaataaag
    42121 cctatgccaa atccattgcc gatcaaattg atatcaacaa agcgaagccg tccagtgaga
    42181 ttcgccctgg caagcttgcg ccttatgaga gtaatcaaac tacccattac tctgtagtgg
    42241 ataaagacgg taacgcggtg gcggtgacct atacgctgaa caccaccttc ggtacgggca
    42301 ttgtcgcggg cgagagcggt attctgctta ataatcagat ggatgatttc tccgccaaac
    42361 cgggcgtacc gaacatttac gggctggtgg gcggtgatgc caacgccgtc gggccgaata
    42421 aacgtccgct gtcatcgatg tcaccaacca ttgtggtgaa agacggtaaa acttggctgg
    42481 ttaccggtag tcctggcggg agtcggatta ttaccaccgt gc
//
LOCUS       DAZPJR010000014        41013 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPJR010000014 DAZPJR010000000
VERSION     DAZPJR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.015"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..262
                     /locus_tag="ACHVB7_000758"
     CDS             <1..262
                     /locus_tag="ACHVB7_000758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912837.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPDPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
     gene            216..1880
                     /gene="asnB"
                     /locus_tag="ACHVB7_000759"
     CDS             216..1880
                     /gene="asnB"
                     /locus_tag="ACHVB7_000759"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR1912838.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2260..2336
                     /locus_tag="ACHVB7_000760"
     tRNA            2260..2336
                     /locus_tag="ACHVB7_000760"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2294..2296,aa:Met,seq:cat)
     gene            2345..2429
                     /locus_tag="ACHVB7_000761"
     tRNA            2345..2429
                     /locus_tag="ACHVB7_000761"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2379..2381,aa:Leu,seq:tag)
     gene            2453..2527
                     /locus_tag="ACHVB7_000762"
     tRNA            2453..2527
                     /locus_tag="ACHVB7_000762"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2485..2487,aa:Gln,seq:ttg)
     gene            2562..2636
                     /locus_tag="ACHVB7_000763"
     tRNA            2562..2636
                     /locus_tag="ACHVB7_000763"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2594..2596,aa:Gln,seq:ttg)
     gene            2652..2728
                     /locus_tag="ACHVB7_000764"
     tRNA            2652..2728
                     /locus_tag="ACHVB7_000764"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Met,seq:cat)
     gene            2777..2851
                     /locus_tag="ACHVB7_000765"
     tRNA            2777..2851
                     /locus_tag="ACHVB7_000765"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2809..2811,aa:Gln,seq:ctg)
     gene            2888..2962
                     /locus_tag="ACHVB7_000766"
     tRNA            2888..2962
                     /locus_tag="ACHVB7_000766"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2920..2922,aa:Gln,seq:ctg)
     gene            complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="ACHVB7_000767"
     CDS             complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="ACHVB7_000767"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR1912839.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4437..5861
                     /gene="miaB"
                     /locus_tag="ACHVB7_000768"
     CDS             4437..5861
                     /gene="miaB"
                     /locus_tag="ACHVB7_000768"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR1912840.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6014..7054
                     /locus_tag="ACHVB7_000769"
     CDS             6014..7054
                     /locus_tag="ACHVB7_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR1912841.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7051..7518
                     /gene="ybeY"
                     /locus_tag="ACHVB7_000770"
     CDS             7051..7518
                     /gene="ybeY"
                     /locus_tag="ACHVB7_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR1912842.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7608..8486
                     /gene="corC"
                     /locus_tag="ACHVB7_000771"
     CDS             7608..8486
                     /gene="corC"
                     /locus_tag="ACHVB7_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR1912843.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8511..10049
                     /gene="lnt"
                     /locus_tag="ACHVB7_000772"
     CDS             8511..10049
                     /gene="lnt"
                     /locus_tag="ACHVB7_000772"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR1912844.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10127..12004)
                     /locus_tag="ACHVB7_000773"
     CDS             complement(10127..12004)
                     /locus_tag="ACHVB7_000773"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR1912845.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12459..13367
                     /gene="gltI"
                     /locus_tag="ACHVB7_000774"
     CDS             12459..13367
                     /gene="gltI"
                     /locus_tag="ACHVB7_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR1912846.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13537..14277
                     /gene="gltJ"
                     /locus_tag="ACHVB7_000775"
     CDS             13537..14277
                     /gene="gltJ"
                     /locus_tag="ACHVB7_000775"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR1912847.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14277..14951
                     /gene="gltK"
                     /locus_tag="ACHVB7_000776"
     CDS             14277..14951
                     /gene="gltK"
                     /locus_tag="ACHVB7_000776"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR1912848.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14951..15676
                     /gene="gltL"
                     /locus_tag="ACHVB7_000777"
     CDS             14951..15676
                     /gene="gltL"
                     /locus_tag="ACHVB7_000777"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR1912849.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15794..16729
                     /gene="rihA"
                     /locus_tag="ACHVB7_000778"
     CDS             15794..16729
                     /gene="rihA"
                     /locus_tag="ACHVB7_000778"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR1912850.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16821..18491
                     /gene="hscC"
                     /locus_tag="ACHVB7_000779"
     CDS             16821..18491
                     /gene="hscC"
                     /locus_tag="ACHVB7_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR1912851.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18551..20005)
                     /gene="djlC"
                     /locus_tag="ACHVB7_000780"
     CDS             complement(18551..20005)
                     /gene="djlC"
                     /locus_tag="ACHVB7_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR1912852.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDDEEEREAFLEA
                     IQAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAK
                     LHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLC
                     AYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTR
                     LLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVV
                     LEAFPKQEMLARLNTRLEGGDTHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKN
                     DEDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(20002..20709)
                     /locus_tag="ACHVB7_000781"
     CDS             complement(20002..20709)
                     /locus_tag="ACHVB7_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1912853.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20811..21365
                     /locus_tag="ACHVB7_000782"
     CDS             20811..21365
                     /locus_tag="ACHVB7_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1912854.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21375..22802)
                     /locus_tag="ACHVB7_000783"
     CDS             complement(21375..22802)
                     /locus_tag="ACHVB7_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR1912855.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22799..23506)
                     /locus_tag="ACHVB7_000784"
     CDS             complement(22799..23506)
                     /locus_tag="ACHVB7_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1912856.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23670..24646
                     /gene="ybeQ"
                     /locus_tag="ACHVB7_000785"
                     /pseudo
     CDS             23670..24646
                     /gene="ybeQ"
                     /locus_tag="ACHVB7_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(24716..25198)
                     /locus_tag="ACHVB7_000786"
     CDS             complement(24716..25198)
                     /locus_tag="ACHVB7_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR1912857.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25433..28015
                     /gene="leuS"
                     /locus_tag="ACHVB7_000787"
     CDS             25433..28015
                     /gene="leuS"
                     /locus_tag="ACHVB7_000787"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR1912858.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28030..28611
                     /gene="lptE"
                     /locus_tag="ACHVB7_000788"
     CDS             28030..28611
                     /gene="lptE"
                     /locus_tag="ACHVB7_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR1912859.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28611..29642
                     /gene="holA"
                     /locus_tag="ACHVB7_000789"
     CDS             28611..29642
                     /gene="holA"
                     /locus_tag="ACHVB7_000789"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR1912860.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29644..30285
                     /gene="nadD"
                     /locus_tag="ACHVB7_000790"
     CDS             29644..30285
                     /gene="nadD"
                     /locus_tag="ACHVB7_000790"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR1912861.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30309..30920
                     /locus_tag="ACHVB7_000791"
                     /pseudo
     CDS             30309..30920
                     /locus_tag="ACHVB7_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31180..31497
                     /gene="rsfS"
                     /locus_tag="ACHVB7_000792"
     CDS             31180..31497
                     /gene="rsfS"
                     /locus_tag="ACHVB7_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR1912862.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31501..31968
                     /gene="rlmH"
                     /locus_tag="ACHVB7_000793"
     CDS             31501..31968
                     /gene="rlmH"
                     /locus_tag="ACHVB7_000793"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR1912863.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31999..33900
                     /gene="mrdA"
                     /locus_tag="ACHVB7_000794"
     CDS             31999..33900
                     /gene="mrdA"
                     /locus_tag="ACHVB7_000794"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR1912864.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33903..35015
                     /gene="mrdB"
                     /locus_tag="ACHVB7_000795"
                     /gene_synonym="rodA"
     CDS             33903..35015
                     /gene="mrdB"
                     /locus_tag="ACHVB7_000795"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR1912865.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35026..36114
                     /gene="rlpA"
                     /locus_tag="ACHVB7_000796"
     CDS             35026..36114
                     /gene="rlpA"
                     /locus_tag="ACHVB7_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR1912866.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36253..37464
                     /gene="dacA"
                     /locus_tag="ACHVB7_000797"
     CDS             36253..37464
                     /gene="dacA"
                     /locus_tag="ACHVB7_000797"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR1912867.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37574..37837
                     /gene="ybeD"
                     /locus_tag="ACHVB7_000798"
     CDS             37574..37837
                     /gene="ybeD"
                     /locus_tag="ACHVB7_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR1912868.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37938..38579
                     /gene="lipB"
                     /locus_tag="ACHVB7_000799"
     CDS             37938..38579
                     /gene="lipB"
                     /locus_tag="ACHVB7_000799"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR1912869.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38839..39792
                     /locus_tag="ACHVB7_000800"
     CDS             38839..39792
                     /locus_tag="ACHVB7_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR1912870.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            40001..40966
                     /gene="lipA"
                     /locus_tag="ACHVB7_000801"
     CDS             40001..40966
                     /gene="lipA"
                     /locus_tag="ACHVB7_000801"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR1912871.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10299 a        10449 c        10291 g         9974 t
ORIGIN      
        1 ccccgtaaaa tataaccatt caataaaacc cgctaaaaat tatcgattca tcaataaata
       61 catcctcaca ccagaccctt tttcaccaat cgacaatcac cattacgttt tttatttttt
      121 caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac
      181 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
      241 atatcaaaac agacgcagtt gagcagcgta agaaagcact cgagctgtca cgcctgatgc
      301 gtcatcgtgg cccggactgg tccggtattt atgccagcga taacgccatt ctcgctcacg
      361 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
      421 cccacgtact ggcggtaaac ggtgaaatct acaaccacca ggcattgcgc gctgaatatg
      481 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
      541 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
      601 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
      661 ggtatgacga acacggtcag ctgtatgtgg cctcagaaat gaaatcgctg gtgccagttt
      721 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagccaggac ggcgaaatcc
      781 gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca
      841 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
      901 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
      961 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccccagttac
     1021 actcctttgc tgtaggtctg ccgggttcac cggatcttaa agcagcccag gaagtggcaa
     1081 accatctggg cacggtgcat cacgaaattc acttcactgt acaggaaggt ctggatgcca
     1141 tccgcgacgt gatttaccac atcgaaactt atgatgtgac cactattcgc gcttcaacac
     1201 cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat taaaatggtg ctgtccggtg
     1261 aaggttctga tgaagtgttt ggcggttatc tttacttcca taaagcgccc aacgctaaag
     1321 aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgcg
     1381 ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaaaaaat
     1441 tccttgatgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaaaatgg
     1501 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc
     1561 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
     1621 cggcgcagca ggtttctgat cagcaactgg aaactgcccg cttccgcttc ccgtacaaca
     1681 cgccaacctc aaaagaagcg tatctgtacc gggagatctt tgaagaactg ttcccgcttc
     1741 cgagcgccgc tgagtgcgtg ccgggcggcc cgtccgtcgc gtgttcttcc gctaaagcga
     1801 ttgagtggga tgaggcgttc aagaaaatgg acgatccgtc tggtcgtgcg gttggcgttc
     1861 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
     1921 gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat
     1981 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccaccaccat
     2041 tgttgaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg gggaaaaaac
     2101 acaaagtaaa caataattga cgaatatagc gccacgctgt tcgcaaccta accaaacagt
     2161 cactttcgag caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat
     2221 gcgccccgca acgccgataa ggtatcgcga aaaaaagatg gctacgtagc tcagttggtt
     2281 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt
     2341 ttttgcggga gtggcgaaat tggtagacgc accagattta ggttctggcg ccgcaaggtg
     2401 tgcgagttca agtctcgcct cccgcaccat tcaccagaaa gcgttgtacg gatggggtat
     2461 cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc
     2521 ccagccatct tcttcgagta agcggttcac cgaccggtta ttggggtatc gccaagcggt
     2581 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatcga
     2641 agaaacaatc tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
     2701 acaggttcga atcccgtcgt agccaccaaa ttctgaatgt gtcgaatacg ttcggcaaat
     2761 tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc accggattct gattccggca
     2821 ttccgaggtt cgaatcctcg taccccagcc aatttattca agacgcttac atcgtaagtg
     2881 caccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
     2941 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc
     3001 tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga
     3061 cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca
     3121 accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta
     3181 acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca
     3241 gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
     3301 ggacaggata actggcccac gcttcaccgt agctgcgggc attcaccaga acatcaatca
     3361 gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat
     3421 gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg
     3481 tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct
     3541 tcgcgatttc cgcctggaat tgcgccatat tcatattctg caactggcga atacgcgccg
     3601 gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc
     3661 cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa
     3721 tcaacatgca cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca
     3781 cctgagaatt tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct
     3841 tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac
     3901 gtaacgttac tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacggtgt
     3961 tttccaccat atagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg
     4021 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg
     4081 catcccagac ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc
     4141 gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta
     4201 ccgaaaatcc gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt cctccgccga
     4261 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
     4321 aagtttacag gatttttacc cccaagggct gataacgctc atactggtca caacggcagc
     4381 aaagcattac actatacgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
     4441 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
     4501 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
     4561 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
     4621 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg
     4681 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
     4741 tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc
     4801 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac
     4861 cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
     4921 cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc
     4981 tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga
     5041 acgtgaacgc ctggcgtggt gaaaactacg acggctccac cggatcgttt gccgatctgc
     5101 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
     5161 tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc
     5221 tgcatctgcc agtacaaagc ggttccgatc gcattctgaa cctgatgggc cgtactcaca
     5281 cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg gatattcaga
     5341 tcagttctga cttcattgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga
     5401 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
     5461 cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag aagcaacgtc
     5521 tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg
     5581 gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg
     5641 ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat
     5701 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta
     5761 ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc
     5821 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
     5881 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
     5941 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
     6001 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
     6061 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct
     6121 cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt
     6181 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca
     6241 gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc gagtactgga
     6301 gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg
     6361 cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga
     6421 cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc
     6481 agttgatgcc ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga
     6541 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct
     6601 gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat
     6661 tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga
     6721 cgcgtttatc attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct
     6781 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct
     6841 gccgcgtaat actaaatcag gcttacgtca cgccatcgaa gtgctggccg atgtcgaaga
     6901 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat
     6961 cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg cgctggcggc
     7021 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
     7081 gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg
     7141 gtgatcccgc agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa
     7201 agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc
     7261 ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt
     7321 caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
     7381 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
     7441 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
     7501 attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga
     7561 ttaacgacga cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
     7621 attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc
     7681 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
     7741 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
     7801 cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
     7861 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
     7921 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
     7981 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg
     8041 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
     8101 tggcgatcgt cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc
     8161 tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc
     8221 gtcagctgag tcgtcatacc tggaccgtgc gtgcactggc ttccattgaa gacttcaacg
     8281 aagcgttcgg cacccacttt agcgatgaag aggttgacac tatcggtggt ctggtgatgc
     8341 aggcatttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca
     8401 aagtggcgat ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact
     8461 caccccagcc gaagctggat gaataaaacc gaaactggat agataactac atggcttttg
     8521 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
     8581 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ttgatggggc
     8641 ttcaggcgtt gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg
     8701 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg
     8761 gaatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt
     8821 ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg
     8881 tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga
     8941 caggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
     9001 caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg
     9061 cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc
     9121 tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg
     9181 tttcgatggt tcagggcgat attccgcaat cactgaaatg ggatggagat cagcttctta
     9241 atacgctgaa gatttactac aacgcaacgg ctccgctgat gggcaaatca tcgttgatta
     9301 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
     9361 tggacggtga gttgcgcgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc
     9421 tcaataagca gaaccgctac gatacctaca acaccatcat cacactgggt aaaggtgcac
     9481 catacagcta cgaatcagcc gatcgctata acaaaaacca tctagtgccg tttggcgagt
     9541 ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt
     9601 cgttcagccg tgggccatat atccagccgc cgctgtcggt aaatggtatt gagcttactg
     9661 cggctatttg ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata
     9721 ccgactatct gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc
     9781 agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca
     9841 ccaacaacgg cattacggcg gtgattggcc cgcaggggga ggttcaggcg atgatcccgc
     9901 agttcacccg cgaggtgttg accactaatg tgacaccgac caccggactc acaccgtatg
     9961 cacgtaccgg caactggcca ctgtgggtgc tgacggcgct gtttggtttt gccgctgtgt
    10021 tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg
    10081 tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga
    10141 tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg
    10201 tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg
    10261 aaaaacgaag gtgattaacg tagctggcag gataaacgcc aataatctgc ccggcattaa
    10321 tattgagcag gatgacataa ccttgcaggc catatgacac caacgctaaa tattgaccgt
    10381 ccgggctaat accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg
    10441 cttgttgatc ccctacgctt atacgtgaaa tcccctgact atcggcaaaa ataacctcgc
    10501 cgttttcagg tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt
    10561 cttctttctg atgcgtgatg gtattgatcc gcacgatgtt acctctgcct gctcccggaa
    10621 ccagtaatgt tctttcatcc ggggaaagtg ccataaccca ggcgcctggt ttaccgctcg
    10681 tccccgtccc gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat
    10741 caatcgctgg cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg
    10801 agaggataag gctgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccctgtcg
    10861 ccacgttaat gaccgataac gcattcgctt caaatgaagt cgcataaatc aacttttcat
    10921 ccgggcttaa tgccagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg
    10981 acttttcttt cgctatgggc gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga
    11041 aaacaacaat gctgttcgtg ttttcaattg cggcatacaa catattacca tcccgactga
    11101 tcgccatatc atggatatcg gcaatctcag cgagcaaatc accactggcc tgttcacgac
    11161 ttaccggagc ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgctgctttt
    11221 tttgccgtcg ctcttgttca atctcctgtg gataaaacgc ttcacgcagg ctttgcctga
    11281 cctgtaaaac tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa
    11341 gactgcccgc aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg
    11401 cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa gcgttatcta
    11461 ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac catacccaac agaggaaata
    11521 ctcgccgctg gtttttatgg tatgtgcccg tacctgaagc aaactgtgtt gctatcgctg
    11581 caccagctat tcccagaatc gccccggatg ccccaacgct gatgactatc tgctcacttt
    11641 cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaggtaa
    11701 tcaggagtcg ccatttcccc agaatctgtt ccgccacgcc tcccacgacc aggaatgcaa
    11761 acatattcat cagcagatga gaaacactac tgtgcagaaa gatactgctc aataagcgcc
    11821 agggttcacc cgacaaagta agaggcgcaa cgtttgcgcc gagatggata agtgcggatt
    11881 gatcgacctc ttcataactc atccattgtg cgagataaca aaatatccca atacagagtg
    11941 caaaaaggat aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag
    12001 acataaggtg tttcttcctt gaaaagacag gtaaaaagca tttttcccgt gtgcagttat
    12061 catcccggca aacgtcccat gccgaaaaga taattctgtc cgaaatatga ctttttcagc
    12121 ctaaaattac aatcgtaatt aattcgcccc taagtatttt ttggcatgta agttgcttta
    12181 tttactgtgt aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag
    12241 cggtagcacc aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg gcgcatctcg
    12301 caaaaacaaa caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac
    12361 aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc acaacgggta
    12421 tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca
    12481 cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg
    12541 cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
    12601 aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
    12661 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
    12721 taaaactgat tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg
    12781 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
    12841 atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt
    12901 ttgccgacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
    12961 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcaca
    13021 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc
    13081 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggaaatc gtcggcaagc
    13141 cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc
    13201 tgatggatga caccaccgcg caggtgcaga cctccggtga agcggaaaaa tggtttgata
    13261 aatggttcaa aaatcccatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg
    13321 aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaactaatta caagaaccag
    13381 gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg
    13441 tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag
    13501 ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt
    13561 atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt
    13621 tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca
    13681 tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat
    13741 gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc
    13801 ccggagctgt tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt
    13861 cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa
    13921 caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg
    13981 ggcctgacgc taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc
    14041 gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact
    14101 attggtctgg tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc
    14161 tgggagtcgt ttaccgctat tacgctggcg tatgtgttga ttaacgcggt tatcatgctg
    14221 gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt
    14281 acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac gggctggtga
    14341 tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg
    14401 cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg
    14461 tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt
    14521 ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg
    14581 tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa
    14641 gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga
    14701 tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg
    14761 gcatcgtact gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc
    14821 gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctgtttgccg
    14881 gatttgttta tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa
    14941 ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct
    15001 gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg
    15061 ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga
    15121 aatcaccgtc gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc
    15181 ccgcgtcggg atggtattcc agcatttcga gctgttccct catctgtcga ttatcgaaaa
    15241 cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc
    15301 attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct
    15361 ttccggcggt cagcagcagc gtgtggcaat cgctcgcgca ttgtgtatgg atcctattgc
    15421 gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga
    15481 cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg
    15541 ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga
    15601 ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag acttcctcgc
    15661 gaaaatcctg cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta
    15721 tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca
    15781 acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc
    15841 tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc
    15901 cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct
    15961 taatcgcacc gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat
    16021 tatcgcggac aatgtgcacg gcgaaagcgg tctggatggc ccggcattac cggaaccgac
    16081 gttcgcaccg caaaactgta cggcggtaga gctgatggcg aaaacgctgc gcgaaagtgc
    16141 ggaacctgtc acgattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag
    16201 ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct
    16261 tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat
    16321 tgtcttccag tcagggatcc cagtggtgat ggccggtctg gatgttactc acaaagcaca
    16381 aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt
    16441 tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg
    16501 tgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac
    16561 tgttgagcgc tgggttggcg tggaaacaca ggggaaatat agccagggta tgacggttgt
    16621 tgattattat tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg
    16681 tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt
    16741 gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctgtt tatctctttt
    16801 tctttcaaga tataaatgca atggataatg cagaactcgc cattggtatc gatctcggca
    16861 ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt ccaaataagt
    16921 tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat attttagtcg
    16981 gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg ttatttaaac
    17041 gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac gcgccagaac
    17101 tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga
    17161 ttaaagatgt ggtgatctcc gttccggctt atttcagcga tgaacaacgc aagcataccc
    17221 gtttagcagc ggagttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag
    17281 ctgcgatggc atatggcctg catacgcaac aaaatacccg ctcgctggtt tttgatctcg
    17341 gtggcggcac gtttgacgtc acggtgctcg aatacgccac gccggtgatt gaagttcacg
    17401 cctccgctgg cgataacttc ctcggtggcg aagattttac ccatatgttg gtcgatgacg
    17461 ttttaaagcg cgcggatgtc gccaggacca cgcttaacga gagtgaactg gcagccttgt
    17521 acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc attgcacatt cgctggcagt
    17581 accaggatga aacgcgggag tgcgaatttt acgagaacga actggaagat ttgtggttgc
    17641 cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgca cgtctgaagc
    17701 cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc
    17761 gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgacccg agcaccattg
    17821 tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagagg
    17881 tgatcctcac tgatatttgc ccttactcgt tgggcgttga agttaaccgc cagggcgttt
    17941 ccggcatttt ctcgccaatt attgaacgaa acaccacagt acccgtgtcg cgtgtagaaa
    18001 cttactcgac catgcacccg gaacaggatt ctattacggt taacgtctat cagggagaaa
    18061 accacaaagt taaaaataac attctggttg aatccttcga tgtgccgttg aagaaaaccg
    18121 gggcttatca gtcgattgat attcgcttta gttatgatat caacggcttg cttgaagttg
    18181 acgtgcttct ggaagatggc agcgttaagt ccagagtcat taatcatagc ccggtaacat
    18241 tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc
    18301 gcgatatgct aatcaaccgc acctttaagg ccaaactgga agagttgtgg gcgcgggcgc
    18361 tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgttgcagt
    18421 caaacgatat ggcccgcgtc gatgaagttc gtcgacgggc gagcgattat ttggctattg
    18481 agatccctta aacacggctg ccggatgcgg cataaacgct gcatccggca tcggcgcaaa
    18541 aaacacatcc tcagctaaac aacatcgcga tttttccgag cgcataacct atcatcagca
    18601 ccgttaagct gattttgata atactggtaa tgctctgtgg tttttcatcc tcatcatttt
    18661 tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttca
    18721 ctttcgtcaa cagcatctgg gtgataatgg cctctaatgg atgagtatct ccaccttcca
    18781 gccttgtatt aagacgggcc agcatctctt gtttcgggaa cgcttccagc acaacctgtt
    18841 ccgctacccg atacaaatcc agtaacggtg aatcttgctc tgtctcgcta ccgactctcg
    18901 atttccacca cgcccatgct ttgcgcttga agaataacga ctcaataaac gattgaccta
    18961 acggcgataa ttttccgcta tgtaaaatct ccgcccataa cagccgcgtc cgggcgctct
    19021 caccaggaac atatttcaac ggtttacctt gcgcatctac catgcttcgc gcttcgataa
    19081 ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat
    19141 tatcagcttg tgtagatggg taactttccc agtacgcgca gagatcggca agcaggcttt
    19201 ccccctcgcc gcagtacaaa cgttgtgcac actggtaata ccgggcatct tcgctttctg
    19261 gttcttccgc ttgccactgt ttggctacag gaatgagttc cgccatacct gtttgcaccg
    19321 agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggtc
    19381 cttccatcgc caaaaacgca gtgacatagt caggatggta acggcagcaa cgttccagcg
    19441 cgaagtaata ttcaacagtc tggttctgta acgcgacggg atattccaga aggctaagga
    19501 aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcat
    19561 ccattccttc gctgtcatta aacgaatgcc agttcaaacg ctctgccaac aaagaagcac
    19621 aatttaatga aagatatcgc gcttctatag cgattgcaca cagcggccag cgtaattgat
    19681 cgacatcctc catgttccag gtatttaatt gctgaataaa tttctgccag gcggagggct
    19741 gaaaacgatc actttctgaa tccagtaatg tcctgaatgc acgtagtatt tcatgttcag
    19801 cggtggcatc tttttcttca tcatcggctt cctcgacagg atttaccgcc agtcgcagcg
    19861 cctcttcata ggcctggcgt aatgctttaa acccctgcgg atcggtttcg ggatgacaca
    19921 acggtaagcg agcaagataa gcctggcgga taatgtctat ttgcgtcgtg ctttcaattt
    19981 cgaggatttg ccaacatgtt ttcataggtc gctcacatcc ttaagcgtta ccgggcattc
    20041 aggctggaca gttaatggtt gccagggaag cgtggcatag aaacggtcgc ttcccgctgc
    20101 cagctcttcg aacatccgac tcccggcgct atcgtatttg tacagggtta gcgcctcgcg
    20161 tagcgaagcg ttgtcttcgc actgagaaga ttgccagtag agacgcccta gtgaataggc
    20221 agaaaaatag tgcatccagc tgtgatagta atggtgagct cgtgcataga cgcgcgattg
    20281 cagccataaa ctctcttctt ccgtcagcca gttgttgagc acaccgaccc gtgacagaaa
    20341 ccccattcga acataatccc aggctttgat tcccccttct ccacagcaca tagcggtttg
    20401 ggcaacgaat tgggtataaa tcaaccctct ctccgagcca ccagcaatta atgctttcct
    20461 ctcttcaggg gaagagcgtt gccatgtcag atataagcca gctaaatcat tggcatgacc
    20521 atcatcagtc atctgataaa tagtctgaat taaatcatca cgattattta ttccccagga
    20581 tgatgagaga tcgaattctt ttatcccagt atacattttt ggcgagtcat gcgcgggttt
    20641 gcaactctga ttaagtattt ccatgggcgc agaaagcgca aaaagaagat attgttcttc
    20701 cttattcatt ttttaataat cccttaagtg cattaactga ctactttttt attattgatg
    20761 agtataaact tgacgctttc aaaataaaaa gaaaatcgaa gcattcacac atgaataaaa
    20821 aatcaatgta tatattcgca atttttatag ttgcagcaat tacctgtatt agccaaccca
    20881 agaaaacgac gttgcgtgat aaagccatgg tgaattatgc ctttgattat ttaagatcac
    20941 caggcagtct accgttaacc acggcagcca cggagctttc cgcgattcat ggtcactcaa
    21001 catcgcaata tcgccttggc gaattttatc ttcatggtag cgacggtaaa ccactggatt
    21061 atacacaggc gagatactgg tatgagcaat cagcagaaca ggaaaatccg cgcgcacaaa
    21121 gcaaactggg gtggatctac ctgaaaggcc tgggagtcaa acccgatacc cgtaaagcaa
    21181 ttctctggta taaggaagca gctgaacaag ggtatgctca tgctcaatat actttgggtt
    21241 tgatctacag aaatggcact ggtattaatg ttaaccatta tgaatctcaa aaatggttaa
    21301 aactggccgc caaacaacat tacaaaaatg cggaaagatt acttgccgga cttcccgcac
    21361 attaacgtcg actgtcaccc gaagagatgg agtatttttc ctatcaaacc ggcaaagata
    21421 aaaatgtaga aaataatttt gatgacgccc agacagccca gcccgccatc gtcaacgggc
    21481 attggctgcg gttgcggctc cggctcgacg gtttcctgac cggtaaaatt cgccgcctga
    21541 attaataatc gacgaataat taatgcttcc agtggacaac gatcttctgc atcaaggcgg
    21601 ctcacaatcg gcattaatga ttcttctgtc ggttggctgg cctgcactaa ttgtttggca
    21661 actaaatata atggccataa gggatgttct gtatcacgcg tgagtgagat ccgcggctta
    21721 cgtcctttgt gcaaaaccat ctcgaccagt gaacgcccca gcgcagagag tttttcatcg
    21781 ctgagtattt cgtataaatg aaaacgcgta cgggcgctgt cgccggggac ataaagtagc
    21841 tgtttaccct gatcgttaac cagttcacga gcttcaatca tcatcaccac taacgggtaa
    21901 taatcgaccc gatgctgacg gcaccattgc aatattaaag catccgcctg ggtggaggga
    21961 tactcgcgcc agtagtcaca aagttctgcc agcaagcttt cgccttctcc acaatagacg
    22021 cgttgcgcgt attcataata acgcgcatct tcattatcag gttcagcttc ccgccacgac
    22081 cgggcgtaat ccagaagttc ggggatatcc aggcgacctg cgctatacca gcggagtaaa
    22141 tttctgtgca gttttgcatc atcggggatg atcactgcgc gatgttgcgc taaatacaat
    22201 gtcagccatt ctggatgcga tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg
    22261 tcattttgca cggcaacggg cagatgcaga atggtctgga agttaaaaac attccccttc
    22321 gcaatggcat ataaaaagga ttccagttct tcttcgtcta tttccccgac gttttcctcc
    22381 tgcaaccacc gcaatctttc tgctaataac accacgcact cgaaggataa atgggcagtg
    22441 ttcatggcga ttgtgcacag cgaccagcgt aattcatcaa tctcttccat cgagcaataa
    22501 tttaattgct gaatgaatcg ctgccaggcg gagggcagaa aacgttcgct atcagaggca
    22561 agtaacgcgc gaaaggcgag cagaatttca tgttctgcta cctcatattc ttctggttgc
    22621 caaacagatt tagcaggcga ctgcgcaatc cggagcgcct cctcatacgc ctgacgaagt
    22681 tgtttaaaac cctgcggatc ggtttctgga tgaaaggaca gtaacagcgc cagataagcg
    22741 cggcggataa tatcgacgtc agtcgtttcc tctatttcga ggatcttcca gcaatttttc
    22801 ataggtcgct catatcctta agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc
    22861 aggacaaggc agggtaatac gcatcatcac tggcaaataa ttgataaaac atatcattgc
    22921 cggaatcgtc gtattcttta cgagctaaga attcgcgtaa taaaggcaga tgatcatctt
    22981 cgggagactg ccaatattgc cgaccaaagg tataaccggc aaagtattgc cgccagttac
    23041 tataataacg tagagcccgt agatgaatac gcgactggat ccatagactt tcttcttcac
    23101 tcaaccagtt attaagtacg cccattcgac ttaaaaaacc catccggaca taatcccatg
    23161 ctttaattcc tccctcacca cagcattcgg ctgtgctggc gacaaattgc gcataagcct
    23221 gaccttgctc atttagttca gcaagatagt cacgccattc acatggcgaa taacgaaacc
    23281 agcggtgata aaaccctgcc aaccgagcgg catgaccgtt atcgatcatt cggtgaattg
    23341 tgcgcagcag gtcatcacgg tcatcaattc cccatgagga agaaaggtca aaaaagcatg
    23401 tacctaaata cattttgggg gatgaatgcg aaggtaaaca gcattcattg cgtatttcca
    23461 tcggagtgga aagtgcaaac aatatttttt gcgaccccat gtccatataa tttatcctta
    23521 aacattcatt taatgaatgt aaactacatt tcgagactca gttatttata cttttctgac
    23581 gcaacataat acgcataaaa taaggcttac gcaaaaatgg aacacataaa aatataatat
    23641 gttttatcta caaccataag gaaatgataa tggtttttac gtcaagttgc tgcgataatt
    23701 tatcaataga tgagattatc gaacgtgctg aaaaaggcga ttgcgaggct caatatattg
    23761 ttgggtttta ttataatcgc gatagcgcaa ttgactctcc ggacgacgaa aaggcctttt
    23821 actggctgaa gctggccgct gagcaaggtc attgtgaagc acagtattcc ttagggcaaa
    23881 agtataccga ggataaaagc cgtcataaag ataatgagca agccatcttc tggctgaaaa
    23941 aagctgccct acaaggccat actttcgctt ccaacgccct tggctggaca ctggatcgtg
    24001 gagaagcccc caattataaa gaagcggttg tctggtatca gatagccgcg gagagcggaa
    24061 tgtcttatgc gcaaaataat cttgggtgga tgtacagaaa tggcaacgga gtcgcaaaag
    24121 actatgcgct ggcatttttc tggtacaaac aagctgcatt acaaggccat agtgacgcgc
    24181 aaaacaatct ggccgatctt tatgaagacg gaaaaggcgt tgctcaaaac aagacactcg
    24241 ccgcattctg gtatttgaaa agcgcacagc agggtaatcg gcacgcccag tttcaaattg
    24301 catgggatta taacgctggc gaaggggtgg accaggacta taagcaagcg atgtactggt
    24361 atctgaaggc tgccgctcag gaaagcgtcg gcgcttacgt caacatcggt tatatgtata
    24421 aacacggaca aggcgttgag aaagattatc aggctgcctt tgaatggttt acgaaagccg
    24481 ctgaatgcaa tgacgccact gcctggtata acctggccat tattatcact acggagaagg
    24541 aagacctgtc gatctccgac aggctctcga cctgtatcgt aaagttcagt catccggaac
    24601 cagagatgtc agtcaggaaa ttcgtgagac tgaagattta ctgtagagat aaaaacaagg
    24661 gccggggaat ttcattcggc ccatattctc tttattgctg tgcgaaatca gactattacg
    24721 gctcaaacgg gcgtctctgg aactggtcat gaccacattt cgggcatagc gtcagcactt
    24781 ccggtgtgta gatcgggaga tggaagtgac atttctcgca gaccagattt cccagcccga
    24841 ccacttctcc gctgtgataa accccatgat gattgaggtc ctggaaaact tcgcgccatt
    24901 caagctgcgt tttatcggtg atatctgcca gctcctgcca taagctttct ttaatcaccc
    24961 gcataaagac gctgtcagat tcttctttca ggctctcttc atagctcatg gcgaactctt
    25021 ccaggtcacg tcttacagct cgcgtcagct catcgacctc ggttcgcgtt aactcccctg
    25081 tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc
    25141 gcaggcgttc gctcagtgac gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg
    25201 tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc cttgctttgc
    25261 taatacgccc acaaactctg taaattaatg tatgaataat ccgggcatca tttcaacgtc
    25321 gagtgcgcga aaggctgttt tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa
    25381 ccacctcaac gctacatttg tagccgtatt gaaaacagga ccactggctg ccatgcaaga
    25441 gcaataccgc ccggaagaga tagaatccaa agtacagctt cattgggatg agaagcgcac
    25501 atttgaagta accgaagacg agagcaaaga gaagtattac tgcctgtcta tgcttcccta
    25561 tccttctggt cgactacaca tgggccacgt acgtaactac accatcggtg acgtgatcgc
    25621 ccgctaccag cgtatgctgg gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg
    25681 tctgcctgcg gaaggcgcgg cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga
    25741 caacatcgcg tatatgaaaa accagctcaa aatgctgggc tttggttatg actggagccg
    25801 cgagctggca acctgtacgc cggaatacta ccgttgggaa cagaaattct tcaccgagct
    25861 gtataaaaaa ggcctggtat ataagaagac ttctgcggtc aactggtgcc cgaacgacca
    25921 gaccgtactg gcgaacgaac aagttatcga cggctgctgc tggcgctgcg ataccaaagt
    25981 tgaacgtaaa gagatcccgc agtggtttat caaaatcact gcttacgctg acgagctgct
    26041 caacgatctg gataaactgg atcactggcc agacaccgtt aaaaccatgc agcgtaactg
    26101 gatcggtcgt tccgaaggcg tggagatcac cttcaacgtt aacgactatg acaacatgct
    26161 gaccgtttac actacccgcc cggacacctt tatgggttgt acctacctgg cggtagctgc
    26221 gggtcatccg ctggcgcaga aagcggcgga aaataatcct gaactggcgg cctttattga
    26281 cgaatgccgt aacaccaaag ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt
    26341 cgatactggc tttaaagctg ttcacccatt aacgggcgaa gaaattcccg tttgggcagc
    26401 aaacttcgta ttgatggagt acggcacggg cgcagttatg gcggttccgg gtcacgacca
    26461 gcgcgactac gagtttgcct ctaaatacgg cctgaacatc aaaccagtta tcctggctgc
    26521 tgacggctct gagccagatc tttctcagca agccctgact gaaaaaggcg tgctgttcaa
    26581 ctctggcgaa ttcaacggtc ttgaccatga agcggccttc aacgccatcg ccgataaact
    26641 gactgcgatg ggcgttggcg agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc
    26701 tcgtcagcgt tactggggcg cgccgattcc gatggtgacg ctggaagacg gtaccgtaat
    26761 gccgaccccg gacgaccagc tgccggtgat cctgccggaa gatgtggtga tggacggcat
    26821 taccagcccg attaaagcag atccggagtg ggcaaaaact accgttaacg gtatgccagc
    26881 gctgcgtgaa accgacactt tcgacacctt tatggagtcc tcctggtact atgcgcgcta
    26941 cacttgccca gagtacaaag aaggtatgct ggattccgaa gcggctaact actggctgcc
    27001 ggtggatatc tacattggtg gtatcgaaca cgccattatg cacctgctct acttccgctt
    27061 cttccacaaa ctgatgcgtg atgcaggcat ggtgaactct gacgaaccag cgaaacagtt
    27121 gctgtgtcag ggtatggtgc tggcagatgc cttctactat gttggcgaaa acggcgaacg
    27181 taactgggtt tccccggttg atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa
    27241 agcgaaagat gcggcaggcc atgaactggt ttataccggc atgagcaaaa tgtccaagtc
    27301 gaagaacaac ggtatcgacc cgcaggtgat ggttgaacgt tacggcgcgg acaccgttcg
    27361 tctgtttatg atgtttgctt ctccggctga tatgactctc gaatggcagg aatccggtgt
    27421 ggaaggggct aaccgcttcc tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa
    27481 aggtgatgtt gcggcactga acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg
    27541 cgatgtgcat aaaacgatcg ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa
    27601 caccgcaatt gcggcgatta tggagctgat gaacaaactg gcgaaagcac caaccgatgg
    27661 cgagcaggat cgcgctctga tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc
    27721 gttcaccccg cacatctgct tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga
    27781 caacgcgccg tggccggttg ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt
    27841 ggtgcaggtt aacggtaaag tccgtgccaa aatcaccgtt ctggtggacg caacggaaga
    27901 acaggttcgc gaacgtgctg gccaggaaca tctggtagca aaatatcttg atggcgttac
    27961 cgtgcgtaaa gtgatttacg taccaggtaa actcctcaat ctggtcgttg gctaagcgcg
    28021 ggaggaagcg tgcgatatct ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc
    28081 gggtgtggct ggcatctgcg tgataccacg caggttcctt ccactatgaa ggtcatgatc
    28141 ctggactcag gcgatccgaa cgggccatta agccgtgcag tgcgtaacca gttacgtctg
    28201 aatggtgtcg agttgcttga taaagaaacc acgcgtaagg acgttccatc cctgcgtttg
    28261 ggtaaagtga gcatcgcgaa agataccgca tcggtattcc gtaacggtca aacagcagag
    28321 tatcagatga tcatgacggt gaatgcgacc gtgttgatcc ccggccgtga tatctacccg
    28381 attagcgcca aagtcttccg ttcgttcttt gataacccgc aaatggcgtt agcgaaagat
    28441 aacgaacaag acatgatcgt aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt
    28501 aagctgccaa gcatccgtgc tgcggatatt cgttccgacg aagaacagac gtcgaccaca
    28561 acggatactc cggcaacgcc tgcacgcgtc tccaccacgc tgggtaactg atgattcggt
    28621 tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg tatcttttac
    28681 ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg
    28741 cacaaggatt cgaagaacac cacacttttt ccattgatcc caacactgac tggaatgcga
    28801 tcttttcgtt atgccaggct atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt
    28861 taccagaaaa cggaccgaat gcggcgatca atgaacaact tctcacactc accggacttc
    28921 tgcatgacga cctgctgttg atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg
    28981 ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc
    29041 aggctcagct tccccgctgg gttactgcgc gcgcaaaaca gctcaactta gaactggatg
    29101 acgcggcaaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg ctggctcagg
    29161 cactggagcg tttatcgctg ctctggccag acggcaaatt gacattaccg cgcgttgaac
    29221 aggcggtgaa tgatgccgcg catttcaccc cttttcattg ggttgatgct ttgttgatgg
    29281 gaaaaagtaa gcgcgcattg catattcttc agcaactgcg tctggaaggc agcgaaccgg
    29341 ttattttgtt gcgcacatta caacgtgaac tgttgttact ggttaacctg aaacgccagt
    29401 ctgcccatac gccactgcgt gcgttgtttg ataagcatcg ggtatggcag aaccgccggg
    29461 gcatgatggg cgaggcgtta aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac
    29521 tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg tgggcagagc
    29581 tgaaagggtt atctcttctg ttgtgccata aacccctggc ggacgtattt atcgacggtt
    29641 gatatgaaat ctttacaggc tctgtttggc ggcacctttg atccggtgca ctatggtcat
    29701 ctaaaacccg tggaaacgct ggcgaatttg attggtctga cgcgggtcac aatcatccct
    29761 aataatgttc ctccgcatcg tccccagccg gaagcgaaca gcgtgcagcg taaacacatg
    29821 cttgaactgg cgattgccga caagccatta tttactcttg atgaacgcga gctaaagcgc
    29881 aatgccccct cttacactgc gcaaacactg aaagagtggc ggcaggaaca aggaccggac
    29941 gtgccgctgg cgtttattat tggtcaggat tcactgctga cctttccgac ctggtacgaa
    30001 tacgaaacga tactcgacaa tgcacatttg atcgtctgtc ggcgtccagg ttacccactt
    30061 gaaatggcgc aaccgcaata ccagcaatgg ctggaagatc atttgacaca taacccggaa
    30121 gatcttcacc ttcagcctgc cggtaaaatt tatctggctg aaacgccgtg gtttaacatc
    30181 tcggcgacca tcatccgcga acgtttgcaa aacggtgaat cgtgtgagga tttattgccg
    30241 gaaccggtac tgacttacat taaccaacaa ggcttgtatc gctgatacct gtcgttacgg
    30301 agggcatgat gcgactgtgg ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc
    30361 tttacagcgg tcatgcgccc acccccctga ccgcgcgcgg tattgagcaa gcgcaaaatc
    30421 tgcatacgct gctacatggt gtttcctttg atctggtttt atgcagtgaa ctggaacggg
    30481 cacagcatac cgcacgactg gttctcagtg accgccagct ccccgtgcaa atcatccctg
    30541 aactcaacga aatgtttttt ggcgactggg agatgcgaca tcatcgcgac ctcatgcaag
    30601 aagatgccga aaactatagc gcgtggtgca atgactggca gcatgcaatc cccacgaacg
    30661 gtgaaggatt tcaggcattt tcgcaacgtg tggaacgctt tatcgcaagg cttagtgaat
    30721 ttcagcacta tcagaatatt ttagtcgtca gccatcaggg tgtactgagt ctgttaatcg
    30781 cccgtttaat tggcatgcct gccgaggcta tgtggcattt tcgcgttgac taggggtgtt
    30841 ggagcgccat tgatatcaac caaaaattcg cgacgctacg cgtcctcaat agccgtgcca
    30901 tcggggtcga gaatgcatga cttttctgtt tttttacggg taagccgcaa cgaccattga
    30961 cagacccggg caggctgata ttctccgcag ccagactttt ttcgccagac acgactttgt
    31021 agaaattgtt ttacaaaaat ggcgatgcaa tctgcggcgc ggggtgggat gatagcccac
    31081 tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc tctggttcag gtatactgac
    31141 agaccatttt tatctatttg attcacccag ggggaaaact tgcagggtaa agcactccag
    31201 gattttgtta tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc cttagacgtt
    31261 caaggcaaat ccagcatcac cgactgcatg atcatctgta cgggtacgtc cagccgtcat
    31321 gttatgtcca ttgctgacca cgttgtgcag gagtctcgcg cagcgggcct gttaccgctc
    31381 ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg atttgggcga tgtgattgtc
    31441 catgtcatgc aggaagagag ccgtcgcctg tatgaactgg aaaaactctg gagttaatgc
    31501 gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca aaccggtttt
    31561 accgagtacc tgcgtcgttt tccgaaagat atgcccttcg agctgattga aattccggcc
    31621 ggaaaacgcg gcaagaatgc ggacatcaag cgcatactcg acaaagaggg tgagcagatg
    31681 ttggcggccg caggcaaaaa ccgcattgtc accctcgata ttccaggcaa gccctgggat
    31741 acgccgcagt tagccgctga gctggaacgc tggaagctgg atggtcgcga cgtcagtcta
    31801 ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag cggcggcgga gcagagctgg
    31861 tcgctgtcgg cgcttaccct cccccatccg ctggttcgcg tgctggtcgc agagagtctg
    31921 taccgggcgt ggagcatcac caccaaccat ccttatcacc gtgagtgata agggagcttt
    31981 gagtagaaaa cgcagcggat gaaactacag aactcttttc gcgactatac ggcagagtcc
    32041 gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga ttttgctgct gaccggcgtg
    32101 cttatcgcca acctgtataa tctgcaaatt gttcgcttta ccgactacca gactcgctct
    32161 aatgaaaacc gcattaagct ggtgcctatc gcgcccagcc gcggcattat ctacgaccgt
    32221 aatggtatcc ctctggccct caaccgcact atctaccaga tagaaatgat gccagagaaa
    32281 gtcgataacg tgcagcaaac gctggacgct ttgcgcagcg tggttgatct gaccgatgac
    32341 gatattgctg cattccgaaa agagcgcgca cgttcacacc gtttcacctc tattccggtg
    32401 aaaactaacc tgaccgaagt acaagtagct cgctttgccg tcaatcagta ccgttttccg
    32461 ggtgtcgaag ttaaaggcta taaacgtcgt tactatcctt acggttcggc gttgacccac
    32521 gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa taatgacggc
    32581 aaactggcca actatgcggc aacgcatgat atcggtaagc tgggcattga gcgttactat
    32641 gaagacgtgc tgcacggtca gaccggttat gaagaggttg aagttaacaa ccgtgggcgt
    32701 gttatccgcc agttaaaaga agtaccaccg caagccggac acgatattta cctgacgctg
    32761 gatctcaaac tccagcaata tattgaaacg ctgctggcgg gtagccgcgc agctgtggtt
    32821 gtcaccgatc cgcgtacagg tggggtgctg gcgctggttt ccacgcctag ttatgaccca
    32881 aacctgtttg ttgacggtat ctccagcaaa gattattccg ccttgttgaa cgccccgaat
    32941 acaccgctgg tgaaccgcgc cacacagggg gtttatcctc ccgcgtctac agttaaaccc
    33001 tatgtggcgg tttcggcatt gagcgccggg gtgatcacgc gcaatacgac gctgtttgac
    33061 ccaggctggt ggcaactgcc aggttcggaa aaacgttatc gtgactggaa aaaatggggc
    33121 cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg cggatacctt cttctatcag
    33181 gtggcctacg atatggggat cgatcgcctc tccgaatgga tgggtaaatt cggttatggt
    33241 cattacaccg gtatcgacct ggcggaagaa cgttccggca acatgcctac ccgcgaatgg
    33301 aaacagaaac gctttaaaaa accgtggtat cagggtgaca ccattccggt tggtatcggt
    33361 cagggttact ggacagcgac cccaatccag atgagtaagg cactgatgat cctgattaat
    33421 gacggtatcg tgaaggttcc tcatttgctg atgagcaccg ccgaagacgg caaacaggtg
    33481 ccatgggtac agccgcatga accgcccgtc ggcgatattc attccggtta ctgggagctg
    33541 gcgaaagacg gtatgtacgg tgttgctaac cgccctaacg gtacggcgca taaatacttt
    33601 gccagcgcac cgtacaaaat tgcggcgaaa tccggtaccg ctcaggtctt cggtctgaaa
    33661 gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt tacgtgacca caaactgatg
    33721 accgcctttg cgccatacaa caatccgcaa gtggctgtcg ccatgattct ggagaacggt
    33781 ggtgcgggtc cggcggttgg tacactgatg cgccagatcc tcgaccacat tatgctgggt
    33841 gataacaaca ccgatctgcc tgcggaaaat ccagcggttg ccgcagcgga ggaccattaa
    33901 tcatgacgga taatccgaat aaaaaaacat tctgggataa agtccatctc gatcccacaa
    33961 tgctgctgat cttactggca ttgctggttt acagcgccct ggttatctgg agcgccagcg
    34021 gtcaggatat tggcatgatg gagcgtaaaa tcggccaaat cgcgatgggt ctagtcatca
    34081 tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc tatctctata
    34141 tcatctgtat tattttgctg gtggcggtag atgctttcgg tgccatctct aaaggtgctc
    34201 aacgctggct ggacctcggt attgttcgtt ttcagccgtc ggaaattgcc aaaatagccg
    34261 taccactgat ggttgcgcgc tttatcaacc gcgacgtttg cccgccatcg ttgaagaaca
    34321 ctggcatcgc gctggtgctg atatttatgc ccacgctgct ggtggctgca cagcctgacc
    34381 tgggaacatc aatcctcgtt gcgctttccg gtctgtttgt actgttcctc tctggcctta
    34441 gctggcgtct gattggcgtc gcagtagtgc tggtggcggc gttcattccg attctgtggt
    34501 tcttcctgat gcatgattac cagcgccagc gcgtaatgat gctcctagac ccggaatcag
    34561 acccactcgg cgcgggctat cacattattc agtctaaaat tgctattggc tccggcggat
    34621 tacgcggcaa aggctggctg cacggcactc agtcacagct tgaatttctc cccgaacgcc
    34681 ataccgactt tatcttcgcg gtactggcgg aagagctggg attagtgggc attctgattc
    34741 tgctcgctct ctacattctg ctgatcatgc gcgggctgtg gatagccgcc agagcgcaaa
    34801 ccacctttgg tcgcgtcatg gctggtggct taatgctgat attattcgtt tatgtcttcg
    34861 taaatattgg tatggtaagc ggtattctgc cggttgtagg ggttccgctc ccactggtca
    34921 gttatggagg atcggcgcta attgtgctga tggctgggtt cgggattgta atgtcaatcc
    34981 acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg taagcagtgg
    35041 ctcgggatct gcatcgcggc aggaatgctc gcggcatgta caagcgatga tggtcagcaa
    35101 cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc ccatagttga aattagcggg
    35161 gcggacccgc gtttcgaacc actgaacgcg acggcaaatc aggattacca gcgcgacggt
    35221 aaaagctaca aaatcgtgca ggatccgtct cgatttagcc aggcgggact ggcggcaatc
    35281 tatgatgccg aacccggcag taacctgacg gcctctggcg aagctttcga tccgacacag
    35341 ctgacggcgg cccatccaac gcttccgatc cccagctacg ccagaatcac taacctggct
    35401 aacgggcgaa tgatcgtggt gcgcattaat gatcgcggcc cttacggcaa cgaccgcgtt
    35461 atttcacttt ctcgcgcggc agctgaccgt cttaacacgt caaacaacac caaagttcgt
    35521 atcgatccga ttattgtcgc ccaggatggt tcgctttctg gtcctggtat ggcttgtacc
    35581 acagtcgcca aacagactta cgccctgcca gcacctcccg atttaagcgg tggcgcggga
    35641 acaagttcag tgtctggccc gcagggtgac attcttccgg tcagtaattc gacgctaaaa
    35701 agcgaagatc cgaccggcgc gccggtaacc agtagcggtt tcctcggcgc tccaacgacc
    35761 ttagcgcctg gtgtactgga aggcagcgaa ccgacgcctg cgccacagcc cgttgttaca
    35821 gctccgtcga caacgcctgc aacctcgcct gcaatggtga caccgcaagc cgcctcgcaa
    35881 agcgccagcg gcaactttat ggtgcaagtc ggggccgtaa gcgatcaggc tcgtgcgcaa
    35941 cagtaccaac agcaactggg acagaagttc ggcgtccccg gtcgcgtaac tcaaaatggc
    36001 gcggtctggc ggatccagct tggcccattc gccagcaaag ccgaagccag taccttgcag
    36061 caacgtttgc aaaccgaagc ccaattacag tcatttatta ctaccgcgca gtagcgtaaa
    36121 gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc ggaaagtcag
    36181 atgcctgccg gtagtgcatt tgctatagta gggcactttt ttaattccat cacggatgtc
    36241 gtagttcaga ccatgaatac cattttttcc gctcgtatca tgaagcgcct ggcgctcacc
    36301 acggctcttt gcacagcctt tatctctgct gcacatgccg atgacctgaa tatcaaaact
    36361 atgatcccgg gtgtaccgca gatcgatgcg gagtcctaca tcctgattga ctataactcc
    36421 ggcaaagtgc tcgccgaaca gaacgcagat gtccgccgcg atcctgccag cctgaccaaa
    36481 atgatgacca gttacgttat cggccaggca atgaaagccg gtaaatttaa agaaactgat
    36541 ttagtcacta tcggcaacga cgcatgggcc accggtaacc cggtgtttaa aggttcttcg
    36601 ctgatgttcc tcaaaccggg catgcaggtt ccggtttctc agctgatccg cggtattaac
    36661 ctgcaatcgg gtaacgatgc ttgtgtcgcc atggccgatt ttgccgctgg tagccaggac
    36721 gcttttgttg gcttgatgaa cagctacgtt aacgcactgg gcctgaaaaa tacccacttc
    36781 cagacggtac atggtctgga tgctgatggt cagtacagct ccgcgcgaga tatggcgctg
    36841 atcggccagg cattgatccg tgacgtaccg aatgaatact cgatctataa agaaaaagaa
    36901 tttacgttta acggtattcg ccagctgaac cgtaacggcc tgttatggga taacagcctg
    36961 aatgtcgacg gcatcaaaac cggacacact gacaaagcag gttacaacct tgttgcttct
    37021 gcgactgaag gccagatgcg cttgatttct gcggtgatgg gcggacgtac ttttaaaggc
    37081 cgtgaagccg aaagtaaaaa actgctaacc tggggcttcc gtttcttcga aaccgttaac
    37141 ccactgaaag taggtaaaga gttcgcctct gaaccggttt ggtttggtga ttctgatcgc
    37201 gcttcgttag gggttgataa agacgtgtac ctgaccattc cacgtggtcg catgaaagat
    37261 ctgaaagcca gctatgtgct gaacagcagt gaattgcatg cgccgctgca aaagaatcag
    37321 gtcgtcggaa ctatcaactt ccagcttgat ggcaaaacga tcgagcaacg cccgctggtt
    37381 gtgttgcaag aaatcccgga aggtaacttc ttcggcaaaa tcattgatta cattaaatta
    37441 atgttccatc actggtttgg ttaaaaattg aacacttgaa agtgtaattt ccgtccccat
    37501 atactaagca tcagtaaaaa actcccgcct tctggcggga gttgctattt aattacgtta
    37561 cgccggagct gacatgaaaa ccaaacttaa cgaactgctt gaattcccta ctccttttac
    37621 ttacaaagtt atggggcagg cgttacctga gctggttgat caggtggttg aagtggtaca
    37681 gcgccatgcg ccaggtgact acaccccaac ggtaaaacca agcagcaaag gcaactacca
    37741 ctcggtatct atcactatca acgccactca tatcgagcag gttgaaacac tgtatgaaga
    37801 actaggcaaa atcgatattg tccgcatggt tctgtaactc gcttctccgt tacccggccc
    37861 ctggtcgggt aactcccctc tcctcgctgt gatatacttt tcccccactt ttactcattc
    37921 tccacggaga tgcagttttg tatcaggata aaattcttgt ccgccagctc ggtcttcagc
    37981 cttacgagcc aatctcccag gctatgcatg aattcaccga tacccgcgat gatagtaccc
    38041 ttgatgaaat ctggctggtc gagcactatc cggtattcac ccaaggtcag gcaggaaaag
    38101 cggagcacat tttaatgccg ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg
    38161 tgacttatca cgggccgggg caacaggtga tgtatgtgtt gcttaacctg aaacgccgta
    38221 aactcggtgt gcgtgaactg gtgaccttgc ttgagcaaac agtggtgaat accctggctg
    38281 aactgggtat agaagcgcat cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga
    38341 aaatttgctc actgggttta cgtattcgac gcggttgttc attccacggt ctggcattaa
    38401 acgtcaatat ggatctttca ccatttttac gtattaatcc ttgtgggtat gccggaatgg
    38461 aaatggctaa aatatcacaa tggaaacccg aagcgacgac taataatatt gcaccacgtt
    38521 tactggaaaa tattttagcg ctactaaaca atcctgactt cgaatatatt accgcttaat
    38581 tgcatacctc aatggcccaa tttacactgg gtcattactc ataatacctg ctaatgcttt
    38641 acatctcctt cgcgtttgta tactttcttt atcacttgca gaaatgcggt gctacgcctt
    38701 gctccgtaac tcaccccatt gattaaccta ttgaaatagt tgattatggt gagcaaaagc
    38761 gttccgttcc aattagacat aagattattg taattttttt aaatattatt tttccatgaa
    38821 taaaattgga gtaagtgcgt ggatagtaat aatcaaattg aaccctgttt aagcagaaag
    38881 tcgtcagaag gtaaaccaca aatatttaca accttacgaa atattgatct taaccttctg
    38941 actatttttg aagctgtata tgtacataaa gggatcgtta atgcagcgaa agtgcttaat
    39001 ctgaccccct cggcaatcag tcagtctatt cagaaactgc gcgttatatt ccctgaccca
    39061 ttgtttattc gcaaaggcca gggtgtcact cctaccgcat ttgcgatgca tctacatgag
    39121 tatatcagtc agggccttga gtccattctt ggcgcgctgg atatcgaagg aagctatgat
    39181 aagcaacgaa cgataactat tgcgaccact ccctcggtcg gagccctggt ccttcctgtc
    39241 atctatcggg cgattaaaac tcactatccg cagcttttac tgcgcaaccc gcccatcagc
    39301 gacgcggaaa accaactcag tcagtttcaa accgatctca tcatcgataa catgttttgc
    39361 accaatcgta cggtgcaaca tcatgttctg ttcaccgaca atatggtgtt aatttgccgc
    39421 gagggaaatc cactactctc tttagaagat gacagagaga ctatcgacaa cgctgcgcat
    39481 gtactcctgt taccggaagg gcaaaatttc agcggtctgc ggcagagagt tcaagagatg
    39541 tttccggacc ggcaaatcaa tttcaccagc tacaacattt tgacaatcgc tgcactggtt
    39601 gccaacagtg acatgttagc gattattccc agccgttttt ataacctgtt tagtcgttgc
    39661 tggccgctgg aaaaactgcc ttttccgtcc ttaaatgagg agcaaataga tttctctatc
    39721 cactacaaca aattcagcct gcgtgatccc atcctgcacg gggtaataga tgttatccgt
    39781 aatgcatttt gaaaaagtat tgttacagcc agatcacagc gcagaacgat aaaatggcac
    39841 aatccaacaa caattttaca ttttcgcgac cgctttggct gctttcaggt ccgtttcaat
    39901 gatatactgc cagtcgttaa ttcaaaaata gttgataatt acaacaatct attgaattga
    39961 aacgctttcc ttcgtaattc gcaactggaa cacgcacgct atgagtaaac ccattgtgat
    40021 ggaacgcggt gttaaatacc gcgatgccga taagatggcc cttatcccgg ttaaaaacgt
    40081 ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg atgaaaatca agcttccagc
    40141 ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt
    40201 ctgcgaggaa gcctcctgcc ctaacctggc ggaatgcttc aaccacggca cagcaacgtt
    40261 tatgatcctc ggcgctattt gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg
    40321 cccggtagct cctgatgcca atgaaccagt gaaactggcg cagaccattg ccgatatggc
    40381 gctgcgttat gtggttatca cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca
    40441 gcactttgcg gattgcatta ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac
    40501 tctggtgccg gatttccgtg gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc
    40561 accagatgtg ttcaaccata acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc
    40621 tggtgcagat tacaactggt cgctgaagct gctggaacgc tttaaagaag cgcatccgga
    40681 aatcccgacc aagtctggtc tgatggtggg actgggtgaa accaatgaag aaattattga
    40741 ggtaatgcgc gacctgcgcc gtcatggtgt gacgatgtta acgctggggc aatatttgca
    40801 gccaagccgc catcacctgc cggttcaacg ttacgttagc ccggatgagt tcgacgaaat
    40861 gaaagccgaa gcgctggcga tgggctttac ccatgctgca tgcggtccgt ttgtccgctc
    40921 ttcttaccac gccgatttgc aggcgaaagg gatggaagtt aagtaatcct gtaataatta
    40981 cttacatctg tctgataaaa aacccgcttt ttg
//
LOCUS       DAZPJR010000015        39820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPJR010000015 DAZPJR010000000
VERSION     DAZPJR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.016"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(101..776)
                     /locus_tag="ACHVB7_000802"
                     /pseudo
     CDS             complement(101..776)
                     /locus_tag="ACHVB7_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(921..1529)
                     /locus_tag="ACHVB7_000803"
     CDS             complement(921..1529)
                     /locus_tag="ACHVB7_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR1912872.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1713..2030)
                     /gene="metJ"
                     /locus_tag="ACHVB7_000804"
     CDS             complement(1713..2030)
                     /gene="metJ"
                     /locus_tag="ACHVB7_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR1912873.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2307..3467
                     /gene="metB"
                     /locus_tag="ACHVB7_000805"
     CDS             2307..3467
                     /gene="metB"
                     /locus_tag="ACHVB7_000805"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR1912874.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3470..5902
                     /gene="metL"
                     /locus_tag="ACHVB7_000806"
     CDS             3470..5902
                     /gene="metL"
                     /locus_tag="ACHVB7_000806"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR1912875.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6041..6175)
                     /locus_tag="ACHVB7_000807"
     CDS             complement(6041..6175)
                     /locus_tag="ACHVB7_000807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912876.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6251..7141
                     /gene="metF"
                     /locus_tag="ACHVB7_000808"
     CDS             6251..7141
                     /gene="metF"
                     /locus_tag="ACHVB7_000808"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR1912877.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7470..9650
                     /gene="katG"
                     /locus_tag="ACHVB7_000809"
     CDS             7470..9650
                     /gene="katG"
                     /locus_tag="ACHVB7_000809"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR1912878.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9744..10649
                     /gene="yijE"
                     /locus_tag="ACHVB7_000810"
     CDS             9744..10649
                     /gene="yijE"
                     /locus_tag="ACHVB7_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR1912879.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10676..11293)
                     /locus_tag="ACHVB7_000811"
     CDS             complement(10676..11293)
                     /locus_tag="ACHVB7_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR1912880.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11567..12670)
                     /gene="gldA"
                     /locus_tag="ACHVB7_000812"
     CDS             complement(11567..12670)
                     /gene="gldA"
                     /locus_tag="ACHVB7_000812"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR1912881.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12681..13343)
                     /gene="fsa"
                     /locus_tag="ACHVB7_000813"
     CDS             complement(12681..13343)
                     /gene="fsa"
                     /locus_tag="ACHVB7_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1912882.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13355..15856)
                     /gene="ptsP"
                     /locus_tag="ACHVB7_000814"
     CDS             complement(13355..15856)
                     /gene="ptsP"
                     /locus_tag="ACHVB7_000814"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1912883.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16165..17244
                     /locus_tag="ACHVB7_000815"
     CDS             16165..17244
                     /locus_tag="ACHVB7_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR1912884.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTGGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17259..17579
                     /locus_tag="ACHVB7_000816"
                     /pseudo
     CDS             17259..17579
                     /locus_tag="ACHVB7_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17630..19926
                     /locus_tag="ACHVB7_000817"
                     /pseudo
     CDS             17630..19926
                     /locus_tag="ACHVB7_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19892..20770
                     /locus_tag="ACHVB7_000818"
     CDS             19892..20770
                     /locus_tag="ACHVB7_000818"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR1912885.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20772..21113
                     /locus_tag="ACHVB7_000819"
     CDS             20772..21113
                     /locus_tag="ACHVB7_000819"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1912886.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21100..21951)
                     /locus_tag="ACHVB7_000820"
     CDS             complement(21100..21951)
                     /locus_tag="ACHVB7_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1912887.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22166..23899)
                     /locus_tag="ACHVB7_000821"
     CDS             complement(22166..23899)
                     /locus_tag="ACHVB7_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR1912888.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24082..26733)
                     /gene="ppc"
                     /locus_tag="ACHVB7_000822"
     CDS             complement(24082..26733)
                     /gene="ppc"
                     /locus_tag="ACHVB7_000822"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR1912889.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27085..28236)
                     /gene="argE"
                     /locus_tag="ACHVB7_000823"
     CDS             complement(27085..28236)
                     /gene="argE"
                     /locus_tag="ACHVB7_000823"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR1912890.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28390..29394
                     /gene="argC"
                     /locus_tag="ACHVB7_000824"
     CDS             28390..29394
                     /gene="argC"
                     /locus_tag="ACHVB7_000824"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR1912891.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29402..30178
                     /gene="argB"
                     /locus_tag="ACHVB7_000825"
     CDS             29402..30178
                     /gene="argB"
                     /locus_tag="ACHVB7_000825"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR1912892.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30239..31612
                     /gene="argH"
                     /locus_tag="ACHVB7_000826"
     CDS             30239..31612
                     /gene="argH"
                     /locus_tag="ACHVB7_000826"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR1912893.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31879..32796
                     /gene="oxyR"
                     /locus_tag="ACHVB7_000827"
     CDS             31879..32796
                     /gene="oxyR"
                     /locus_tag="ACHVB7_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR1912894.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPIADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32779..34179)
                     /gene="sthA"
                     /locus_tag="ACHVB7_000828"
     CDS             complement(32779..34179)
                     /gene="sthA"
                     /locus_tag="ACHVB7_000828"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR1912895.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34500..35150
                     /gene="fabR"
                     /locus_tag="ACHVB7_000829"
     CDS             34500..35150
                     /gene="fabR"
                     /locus_tag="ACHVB7_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR1912896.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35150..35509
                     /locus_tag="ACHVB7_000830"
     CDS             35150..35509
                     /locus_tag="ACHVB7_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR1912897.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35549..36649)
                     /gene="trmA"
                     /locus_tag="ACHVB7_000831"
     CDS             complement(35549..36649)
                     /gene="trmA"
                     /locus_tag="ACHVB7_000831"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR1912898.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36763..36953
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37018..38862
                     /gene="btuB"
                     /locus_tag="ACHVB7_000832"
     CDS             37018..38862
                     /gene="btuB"
                     /locus_tag="ACHVB7_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR1912899.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38807..39664
                     /gene="murI"
                     /locus_tag="ACHVB7_000833"
     CDS             38807..39664
                     /gene="murI"
                     /locus_tag="ACHVB7_000833"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1912900.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9074 a        10023 c        10993 g         9730 t
ORIGIN      
        1 gcagaatgag gtaaaaatct ttggctgtgt attcagtctg gtaatcatcc aaggaaagct
       61 tatcagaagg gtgatgactc caattagtag aacatatatt ttcgataaaa gcaccgatga
      121 ctttttcatg aagttctaca gaacgcgaga aacagatggt tctgcgggcc agacgtttga
      181 ttcgggttct cagggtcaga ttgttacgct caatgtgctg agtaaaaatc ttaccagtca
      241 gatgcttctc cttcggtaac tctctagcgt aactacccca ttcgtcagtg gtcaacatgc
      301 cgatggtaaa agggtaagca gcgcccgcaa ttcacgacag gtttcgtcag tccgggggcc
      361 aaaagtgtag gccagaacac caccagtttt cgtgttgtac acgtaccaga gccagtgctg
      421 tcgcgcttta ctgccgacaa agctccattg ctgactaagt ttgcagatga gtgccacatc
      481 agcatgagcg accggggatg acgttattct tcgtggcgag agttttttaa agttcggatg
      541 actgtattaa tgccgatttt cagcgtacgg gcagtatcgc gaaccccggc accgttgaaa
      601 gtcatttcag cgatttggtc tttaactccc ggtttacggg gctcgtaaca gtaactgagc
      661 tgaaataccc ggtgacagtc acggccgcgg aatcgctcat gtctttttgg gttctgcccg
      721 tgacggtaaa cctgaacgga ctggcaacgg gggcaatgaa ggttaactgt gaccatgaaa
      781 caaccttaaa agcgagtatt atatccttaa ttcaactaat tggaggcatc accgtttacc
      841 ggagcgacag cttgcggaaa ggtcgtgcaa ctacaaagca acaaacagac tacggcggga
      901 attatttttt tcatcatcca ttaccacgaa aacagcggcc actctttggc gactgtggtg
      961 agttgtggcg cgtcgaaaac ggcttgtggt gaaactaccg tttcctcaag cggaatattt
     1021 ttgccgtagc gttctttgag cttagcctgg accagcgggt tgctgagatc gaactctttc
     1081 aacttctgct gctcgcccac gcgcattccc agcgcgttct ggtaaacctt ccacaccact
     1141 tccgaacagt actggcgatc gtcgctccat gagaagctga aatcatacgg tttaccgaga
     1201 taacgttttg ccgtttgcgc cagtttttgc tgttgttcaa cactcagtcc gccttcaacg
     1261 cggcgaacaa cgtatttgcc cttttcacca tgggcgatcc actgcttgag cggggggtat
     1321 ttcaccgggc cgaccgcttc aaaaacgtag ggctttttgt tgcgtatcac cagcataccg
     1381 gtgtggctat aatcggaatg ggtcgccagt tggatcgctt tactttgcga tgagcgagag
     1441 atctgaaaga tgatgtcgcc ggtttgtggc tgccaggcaa aggcaggtac agaaaccagc
     1501 aggctgagga tcagcagcct gtttttcata gttaaacgtc catgtataaa aagcggtggg
     1561 tcgcagacaa cgtgctcgtt gtttatgccg gatgcggcgt gaacgcctta tccggcctac
     1621 aagttcgtgc aaattcaata aattgcaata tgacgtaggc ctgataagcg tagcgcatca
     1681 ggcgattcca ctccgcgccg ctcttttttg ctttagtatt cccacgtctc cgggttaatc
     1741 cccatctcac gcatgatctc ttttgccgct tccgggattt cgtcgctgcg ctctttacgc
     1801 agatcggcat catccggcaa aggttgcccg gtaaaggcat gcagaaacgc ttcgcacagc
     1861 agctcgctgt tggtagcgtg acgcaggttg ttcacctgac gacgcgtgcg ttcatcggtg
     1921 aggattttta acaccttaag aggaatggaa accgtaatct ttttgacttg ttcactcttc
     1981 ttgccgtgct cagcgtatgg gctgatatat tcgccgctcc attcagccat gagatactta
     2041 atcctcttcg tcaataaatt gagaccagac cacacaattt atgtgggtac tgaccgtaaa
     2101 cccgcatagt ttaccgtaca ggcgttaccg tgacatcgtg taatgcacct gtcggcgtga
     2161 taatgcatat aattttaacg gctatttggg atttgctcaa tctatacgca aagaagttta
     2221 gatgtccaga tgtattgacg tccattaaca caatgtttac tctggtgcct gacatttcac
     2281 cgacaaagcc caggaaactt catcacatga cgcgtaaaca ggccaccatc gcagtgcgta
     2341 gcgggttaaa tgacgacgaa cagtatggtt gcgttgtccc tcccatccat ctttccagca
     2401 cctataactt taccggattt aatgaaccgc gcgcgcatga ttactcgcgt cgcggcaacc
     2461 caacgcgcga tgtggttcag cgtgcgctgg cagaactgga aggcggtgct ggcgcagtac
     2521 ttactaatac cggcatgtcc gcgattcacc tggtaacgac cgtctttttg aaacctggcg
     2581 atctgctggt tgcgccgcac gactgctacg gcggtagcta tcgcctgttc gacagtctgg
     2641 cgaaacgcgg ttgctatcgc gtgttgtttg ttgatcaagg cgatgaacag gcattacggg
     2701 cagcgctggc agaaaaaccc aaactggtac tggtagaaag cccaagtaat ccattgttac
     2761 gcgtcgtgga tattgcgaaa atctgccatc tggcaaggga agtcggggcg gtgagcgtgg
     2821 tggataacac cttcttaagc ccggcattac aaaatccgct ggcattaggt gccgatctgg
     2881 tgttgcattc atgcacgaaa tatctgaacg gtcactctga cgtggtggct ggcgtggtga
     2941 ttgctaaaga cccggacgtt gtcactgaac ttgcctggtg ggcaaacaat attggcgtga
     3001 cgggcggcgc gtttgacagc tatctgctgc tacgcgggtt gcgaacgctg gtgccgcgta
     3061 tggagctggc gcagcgcaac gcgcaggcga ttgtgaaata tctgcaaacc cagccgttgg
     3121 tgaaaaaact gtatcacccg tcgctgccgg aaaatcaggg gcatgaaatt gccgcgcgtc
     3181 agcaaaaagg ctttggcgca atgttgagtt ttgaactgga tggcgatgag cagacgctgc
     3241 gtcgtttcct gagcgggctg tcgttgttta cgctggcgga atcattaggg ggagtggaaa
     3301 gtttaatctc tcacgccgca accatgacac atgcaggcat ggcaccagaa gcgcgtgctg
     3361 ccgccgggat ctccgagacg ctgctgcgta tctccaccgg tattgaagat ggcgaagatt
     3421 taattgccga cctggaaaat ggcttccggg ctgcaaacaa ggggtaaaaa tgagtgtgat
     3481 tgcgcaggca ggggcgaaag gtcgtcagct gcataaattt ggtggcagta gtctggctga
     3541 tgtgaagtgt tatttgcgtg tcgcgggcat tatggcggag tactctcagc ctgacgatat
     3601 gatggtggtt tccgccgccg gtagcaccac taaccagttg attaactggt tgaaactaag
     3661 ccagaccgat cgtctctctg cgcatcaggt tcaacaaacg ctgcgtcgct atcagtgcga
     3721 tctgattagc ggtctgctac ccgctgaaga agccgatagc ctcattagcg cttttgtcag
     3781 cgatctggaa cgcctggcgg cgctgctcga cagcggtatt aacgacgcag tgtatgcgga
     3841 agtggtgggc cacggggaag tatggtcggc acgcctgatg tctgcggtac ttaatcaaca
     3901 agggctgcca gcggcctggc ttgatgcccg cgagttttta cgcgctgaac gcgccgcaca
     3961 accgcaggtt gatgaagggc tttcttaccc gttgctgcaa cagctactgg tgcaacatcc
     4021 gggcaaacgt ctggtggtga ccgggtttat cagccgtaac aacgccggtg aaacggtgct
     4081 gctggggcgt aacggttccg actattccgc gacacaaatc ggtgcgctgg cgggtgtttc
     4141 tcgtgtaacc atctggagcg acgtcgccgg ggtatacagt gccgacccgc gtaaagtgaa
     4201 agatgcctgt ctgctgccgt tgctgcgtct ggatgaagcc agcgaactgg ctcgtctggc
     4261 ggctcccgtt cttcacgccc gtactttaca gccggtttct ggcagcgaaa tcgacctgca
     4321 actgcgctgt agctacacgc cggatcaagg ttccacgcgc attgaacgcg tgctggcctc
     4381 cggtactggt gcgcgtattg tcaccagcca cgatgatgtc tgtttgattg agtttcaggt
     4441 gcccgccagt caggatttca aactggcgca taaagagatc gaccaaatcc tgaaacgcgc
     4501 gcaggtacgc ccgctggcgg ttggcgtaca taacgatcgc cagttgctgc aattttgcta
     4561 cacctcagaa gtggccgaca gtgcgctgaa aatcctcgac gaagcggggt tacctggcga
     4621 actgcgcctg cgtcaggggc tggcgctggt ggcgatggtc ggtgcaggcg tcacccgtaa
     4681 cccgctgcat tgccaccgct tctggcagca actgaaaggc cagccggtcg aatttacctg
     4741 gcagtccgat gacggcatca gcctggtggc agtactgcgc accggcccga ccgaaagcct
     4801 gattcagggg ctgcatcagt ccgtcttccg cgcagaaaaa cgcatcggcc tggtattgtt
     4861 cggtaagggc aatatcggtt cccgttggct ggaactgttc gcccgtgagc agagcacgct
     4921 ttcggcacgt accgactttg aatttgtgct ggcaggcgtg gtggacagcc gccgcagtct
     4981 gttgagctat gacgggctgg acgccagccg cgcgttagcc ttcttcaacg atgaagcggt
     5041 tgagcaggat gaagagtcgt tgttcctgtg gatgcgcgcc catccgtatg atgatttagt
     5101 ggtgctggac gttaccgcca gccagcagct tgctgatcag tatcttgatt tcgccagcca
     5161 cggtttccac gtcatcagcg ccaacaaact ggcgggagcc agcgacagca ataaatatcg
     5221 ccagatccac gacgccttcg aaaaaaccgg gcgtcactgg ctgtacaatg ccaccgtcgg
     5281 tgcgggctta ccgatcaacc acaccgtgcg cgatctgatc gacagcggcg atactatttt
     5341 gtcgatcagc gggatcttct ccggcacgct ctcctggttg ttcctgcaat tcgacggtag
     5401 cgtgccgttt accgagctgg tggatcaggc gtggcagcag ggcttaaccg aacctgaccc
     5461 gcgtgacgat ctctctggca aagacgtgat gcgcaagctg gtgattctgg cgcgtgaagc
     5521 aggttacaac atcgaaccgg atcaggtacg cgtggagtcg ctggtgcctg cccattgcga
     5581 aggcggcagc atcgaccatt tctttgaaaa tggcgatgaa cttaacgagc agatggtgca
     5641 acggctggaa gcggcccgcg aaatggggct ggtgctgcgc tacgtggcgc gtttcgatgc
     5701 caatggcaaa gcgcgtgtgg gcgtggaagc ggtgcgtgaa gaccatccgc tggcatcact
     5761 gctgccgtgc gataacgtct ttgccatcga aagccgctgg tatcgcgata atccgctggt
     5821 gatccgtggg cctggcgctg ggcgcgacgt caccgccggg gcgattcagt cggatatcaa
     5881 ccggctggca cagttgttgt aatttcagaa atttaataat gcccggtact catgttttcg
     5941 ggtttatggt ttctaatgaa atatattgaa ttatcatagg attaggccgg attaagcgtt
     6001 tacgacgaat ccggcaagaa gcaataagta catggttagt ttatatttgc agtccggttt
     6061 gctttgcata ccggattttc tttttcttac catcctgaag ttttttcatc ttccctgatt
     6121 tttcctcacc atcattggtc atttttcggt tgacgccctt cggcttttcc ttcatcttta
     6181 catctggacg tctaaacgga tagatgtgca caacacaaca tataactaca agcgattgat
     6241 gaggtaaggt atgagctttt ttcacgccag ccagcgggat gccctgaatc agagcctggc
     6301 agaagtccag gggcagatta acgtttcgtt cgagtttttc ccgccgcgta ccagtgaaat
     6361 ggagcagacc ctgtggaact ccatcgatcg ccttagcagc ctgaaaccga agtttgtatc
     6421 ggtgacctat ggcgcgaact ccggcgagcg cgaccgtacg cacagcatta ttaaaggcat
     6481 taaagatcgc actggtctgg aagcggcacc gcatcttact tgcattgatg cgacgcccga
     6541 cgagttgcgc accattgcac gcgactactg gaataacggt attcgtcata tcgtggcgct
     6601 gcgtggcgat ctgccgccgg gaagtggtaa gccagaaatg tatgcttctg acctggtgac
     6661 gctattaaaa gaagtggcag atttcgatat ctccgtggcg gcgtatccgg aagttcaccc
     6721 ggaagcaaaa agcgctcagg cggatttgct taatctgaaa cgcaaagtcg atgccggagc
     6781 caaccgcgcg attactcagt tcttcttcga tgtcgaaagc tacctgcgtt ttcgtgaccg
     6841 ctgtgtatcg gcgggcattg atgtggaaat tattccggga attttgccgg tatctaactt
     6901 taaacaggcg aagaaatttg ccgatatgac caacgtgcgt attccggcgt ggatggcgca
     6961 aatgttcgac ggcctggatg atgatgccga aacccgcaaa ctggtgggcg cgaatattgc
     7021 catggatatg gtgaagattt taagccgtga aggagtgaaa gatttccact tctatacgct
     7081 taaccgcgct gaaatgagtt acgcgatttg ccatacgctg ggtgttcgac ctggtttata
     7141 aatagtgtgg cttttgtgaa aatcacacag tgatcacaaa ttttaaacag agcacaaaat
     7201 gctgcctcga aatgagggcg ggaaaataag gttgtcagcc ttgttttctc cctcattact
     7261 tgaaggatat gaagctaaaa ccctttttta taaagcattt gtccgaattc ggacataatc
     7321 aaaaaagctt aattacgatc aatttgacct acatctcttt aaccaacaat atgtaagatc
     7381 tcaactatcg catccgtgga ttaattcaat tataacttct ctctaacgct gtgtatcgta
     7441 acggtaacac tgtagagggg agcacattga tgagcacgtc agacgatatc cataacacga
     7501 cagccactgg caaatgcccg ttccatcagg gcggtcacga ccagagcgcg ggggcgggca
     7561 caaccactcg cgactggtgg ccaaatcaac tccgtgttga cctgttaaac caacattcta
     7621 atcgttctaa cccgctgggt gaggactttg actaccgcaa agaattcagc aaattagatt
     7681 actacggcct gaaaaaagat ctgaaagccc tgctgacaga atctcagccg tggtggccag
     7741 ccgactgggg cagctacgcc ggtctgttta ttcgtatggc ctggcacggc gcaggtactt
     7801 accgttcaat cgatggacgc ggcggcgcgg gtcgtggtca gcaacgtttt gcaccgctga
     7861 actcctggcc ggataacgta agcctcgata aagcgcgtcg cctgttgtgg ccaatcaaac
     7921 agaaatatgg tcagaaaatc tcctgggccg acctgtttat cctcgcaggt aacgtggcgc
     7981 tggaaaactc cggcttccgt accttcggtt ttggtgccgg tcgtgaagac gtctgggaac
     8041 cggatctgga tgttaactgg ggtgatgaaa aagcctggct gactcaccgt catccggaag
     8101 cgctggcgaa agcaccgctg ggtgcaaccg agatgggcct gatttacgtt aacccggaag
     8161 gcccggatca cagcggcgaa ccgctttctg cggcagcagc tatccgcgcg accttcggca
     8221 acatgggcat gaacgacgaa gaaaccgtgg cgctgattgc gggtggtcat acgctgggta
     8281 aaacccacgg tgccggtccg acatcaaacg taggtcctga tccagaagct gcaccgattg
     8341 aagaacaagg tttaggctgg gcgagcactt acggcagcgg cgttggcgca gatgccatta
     8401 cctctggtct ggaagttgtc tggacccaga cgccgaccca gtggagcaac tatttcttcg
     8461 aaaacctgtt caaatatgag tgggtacaga cccgcagtcc ggctggcgca atccagttcg
     8521 aagcggtaga cgcaccggaa attatcccgg atccattcga tccgtcgaag aaacgtaaac
     8581 cgacgatgct ggtgaccgac ctgacgctgc gttttgatcc tgagttcgag aagatctctc
     8641 gtcgtttcct caacgatccg caggcgttca acgaagcctt tgcccgtgcc tggttcaaac
     8701 tgacgcacag ggatatgggg ccgaaatctc gctacatcgg gccggaagtg ccgaaagaag
     8761 atctgatctg gcaagatccg ctgccgcagc cgatctacaa cccgaccgag caggacatta
     8821 tcgatctgaa attcgcgatt gcggattctg gtctgtctgt tagtgagcgg gtatcggtgg
     8881 cctgggcatc tgcttctacc ttccgtggtg gcgacaaacg cggtggtgcc aacggtgcgc
     8941 gtctggcatt aatgccgcag cgcgactggg atgtgaacgc cgcagccgtt cgtgctctgc
     9001 ctgttctgga gaaaatccag aaagagtctg gtaaagcctc gctggcggat atcatcgtgc
     9061 tggctggtgt ggttggtgtt gagaaagccg caagcgccgc aggtttgagc attcatgtac
     9121 cgtttgcgcc gggccgcgtt gatgcgcgtc aggatcagac tgacattgag atgttcgagc
     9181 tgctggagcc aattgctgac ggtttccgta actatcgcgc tcgtctggat gtttccacca
     9241 ccgagtcact gctgatcgac aaagcccagc aactgaccct gaccgcgccg gaaatgaccg
     9301 cgctggtggg cgggatgcgc gtactgggtg ccaacttcga tggcagcaaa aacggcgtct
     9361 tcactgaccg cgttggcgta ttgagcaatg acttcttcgt gaacttgctg gatatgcgtt
     9421 acgagtggaa agcgaccgac gaatcgaaag agctgtttga aggccgtgac cgcgaaaccg
     9481 gcgaagtgaa atacactgcc agccgtgcgg atctggtatt tggttctaac tctgtcctgc
     9541 gtgcggtggc tgaagtttac gccagcagcg atgctcacga gaagtttgtt aaagacttcg
     9601 tggcggcatg ggtgaaagtg atgaacctcg accgtttcga cctgctgtaa tctgaccttc
     9661 ttcagcgact gcctttcagg cagtcgctga agtttcttta ccggcgtata gtgtccacag
     9721 gaaaactaca cactggatct ctcatgtctg ccgcaggaaa gagcaaccca ctggcaatca
     9781 gtggcctggt tgtgctcaca cttatctgga gttatagctg gattttcatg aagcaagtca
     9841 ccagctacat cggtgccttc gactttaccg ccttacgctg cattttcggc gctctcgttt
     9901 tattcatcgt ccttttatta cgtggtcgcg gaatgcgccc gacaccgttt aaatacacct
     9961 tagccattgc cctgttacaa acctgcggga tggttggcct ggcgcagtgg gcgctggtca
    10021 gcggcggagc ggggaaggtg gcgatcctaa gctataccat gccgttctgg gtggtgattt
    10081 tcgccgcgtt gtttctcggt gaacgcctgc gacgtgggca atatttcgcg atactgattg
    10141 ccgctttcgg cttatttttg gtgttgcagc cgtggcaact cgatttctct tcgatgaaaa
    10201 gtgccatgct ggcaatcctc tccggcgtca gttggggggc gagcgcgatt gttgctaaac
    10261 gtctgtatgc ccgtcatccg cgcgtggatt tattgtcgtt aacatcctgg cagatgctct
    10321 atgcggcgct ggtgatgagt gtggtcgctt tactggtgcc gcaacgtgaa attgactggc
    10381 agcccaccgt gttctgggcg ctggcctaca gtgcgattct ggcgacggca ctggcgtgga
    10441 gcttatggtt gtttgtattg aaaaacttgc ctgccagtat tgccagctta agcacactgg
    10501 ccgttcccgt ttgcggcgta ctcttttcct ggtggctgct cggcgagaat ccgggggccg
    10561 ttgaaggtag cggtattgtg ctgattgtga tggcactggc gctggtgagc cgtaagaaaa
    10621 aagaagccgt tagtgtaaaa aggatctgaa ttttttcttc atgtggggcg atcgcttatt
    10681 taacaaaata acgataatgc cccaccatgc gccagctaaa cagcacatct tcttcctgcg
    10741 cacctgcgcc aatgttatgt atcaccagcg gcgtaccgtc gcgggcgaag ccatctgaaa
    10801 ccaccccaat atgtgccagc ccgttatcta atcgccagga gacaatatcg cccgcttgat
    10861 agtcactggg gttcttgctg atggggcgtg ttttatcgtg gtgggtaaac caggtttcca
    10921 gattaggcac ccgacggtga tcgatgttgc tgtccgggcg ctttaactgc catttttgcg
    10981 ggtactcggc aaaattcttc gccatatctt cgtgcaccag tttctgcaaa tcgaccttct
    11041 ggctgcgcaa tgcacggatc accacatcgg aacatacccc gcgtttttgc gggacatcac
    11101 cgccaggata ggtaagctgc atatacgccg gatcgtaaaa tagcgtgctg ccgatttgct
    11161 gtctggcacc gtctgcgatg gcaaggttgg tattggcctg gatttgtacc acggttggtg
    11221 gaacggcggg agattttaag gagtggctgg taaatgccgt tagcaggctg agcagcgcca
    11281 gtgaagcttt catctggttc gtccgtgaaa ataagagtca cagattcgac cttccgggat
    11341 aacgattgtt ctgtagagaa aatgtccaga agcggataaa aaactcctga acaacgtggg
    11401 agtttcagat tactggcaca gagtgccgga tgcggcgtga acgccttatc tgtcctaaaa
    11461 aaaaagaaaa ttcaatatat tgcagagatt gcgtaggcct gataagcgta gcgcatcagg
    11521 caattttgcg ttcaaactcc cggacaagcc gggagtttgg agtaggttat tcccactctt
    11581 gcaggaaacg ctgaccgtac tggtcggcta ccagcagagc ggcgtaaacc tgatctggcg
    11641 tcgcgccgcc aggcatgttg tggatggttt cacattctgc acatgccgct tcagccacaa
    11701 ttcgcatttt cgccgggaca tcttctttaa tatccagttg agcgagagtt attggcaaac
    11761 ctaccgcatg gctaagtgca gctacggttt cgatttcctc aaccggtgcg ttttccagaa
    11821 ccagctgcgt cagcgtacca aatgccactt tttcaccgtg ataatagtga tgcgcgtccg
    11881 ggatagcggt caggccgtta tgcactgcgt gcgccgcagc cagaccacca ctttcaaaac
    11941 caacaccgct caaataggtg ttcgcttcaa tcacgcgctc cagcgccgga gtcactacat
    12001 gctgttcggc agcaagcatc gctttttcgc cttcttccag cagggtgttg tagcacagtt
    12061 cagccagtgc cagcgcagcc tgggtgcact tgccgcccgc catggtggtc gcgccgctac
    12121 gagagcaggc acgcgcttca aaccaggttg ccagcgcatc gccgataccc gccgctaaca
    12181 gacgtgcagg tgcgccagcg acgattttgg tgtcgacaat gaccatattc gggttatttg
    12241 gcaacagcag atagcggtca aactcaccct catcggtgta gataacagac aatgcgctgc
    12301 acggtgcatc ggtagaggcg atagtcggtg cgatcgctac cggaacaccc atgaaatgtg
    12361 ccagtgcttt ggctgtatcg agggtttttc cgccaccgat accgagaatt gcgccacact
    12421 gcgcagtctc cgcgatgcca cgcagacggt cgatctcatt ttgcgaacat ccaccgccaa
    12481 acggcgcaat ttctactacc agtccagcat ctttaaagct tttctcgaca gtggattgag
    12541 caaaacctaa aacaaatttg tcacccacca ctaaccagcg ttctgccagc ggcttcaggt
    12601 attcgcccag acgattaatc acatcagcgc cctggatgta tttacccggt gattgaataa
    12661 tgcggtccat aattgctcct ttagagatga gtagtgccaa atgcggcatt ccagtcgtgt
    12721 tcgaacttct ctatagctga ctctaccgca ggggtgttga gcatttgttg cgctacatct
    12781 aagggcaggg tgatggattc acatcctgcc agtaaacagt ccagcgcctg acgcggcgtt
    12841 ttaaagctgg ctgccagcac catgctttct ggcgcgtgca tttctaacag cgcttgcagc
    12901 tcctgaaccg tacgaatgcc gtctccgccc tgggcatcta cgcggttaac atacggagca
    12961 acgtattttg cccctgccag cgcggccagt aacccttgtg cagcactgta cactgcggtg
    13021 ccaagcgtag taataccctc ttttttcagt attttaattg ctgccagacc ttcggaagtc
    13081 accgggattt tcaccacaat acccggaata gcgtcgcgca ggcgcttcgc ttcttccacc
    13141 atcccctgcg cgtcgcggct catggtctga gcaaacagaa tgccctcatc accaatcgct
    13201 ttttgcagac gcggcagcac ttcccatatg gactccttgc tggcagcgat aatgctcggg
    13261 ttagttgtca ccccggcaat ggggaatatg cgtgccagac gttcgacttc tgcgacgtta
    13321 gcggtgtcca gatacagttc catgatgttt ccttttacag ttccagttca tgttgcagca
    13381 ggctggcgat agcgtcggca gaggcggcgt taaccagcgc gttacggaat tcttcgtgca
    13441 tgatgcggcg agcgaggcgc gagaaaatgc gcatgtgctg atcgcccgca gcgtgtttgt
    13501 tcagggttaa catgatgatg aattgcgctt catcatcacc ccagcgcacc ggcgcttgca
    13561 gacgcgcaac gctgatggtg gattgctcaa tgtgttctga tttgctgtgt ggaatggcaa
    13621 aactaaagcc cagaccggta gagaaaacgg cctcgcgcgc ccacaagtcg gcttccagtt
    13681 tacgcggata gcggcagcgc cctgccagca gcaggttatc ggtcatgcct ttgagcactt
    13741 cttctttgct gcgccagtcg ctttccagtg tgatgcactc ggcggtgacc agcggtgcgt
    13801 cttgttgggt catgcggaat tgcgccagca ggtgctccac ttccagcgaa gtacggcagg
    13861 ccattgcctg gttgagcaac ttgcggcact cacggctatc aagttgcgcc atccgcgctt
    13921 tcgccgccgg aattgatggt gcgctcatgc tgagttcatc cagccctaag ccgaccagca
    13981 acggcagcac ggaacctttc gctcccagct caccgcacag accaatccat ttaccctggc
    14041 gatgcaccgc ctgcacggcg taatcgagcg cccgcaagaa tgccggattc aggctgttgt
    14101 agtgacgagt aaccttagcg ttatcgcgat ccactgccag cagatattgc gtcaggtcat
    14161 tactaccaat actaaagaaa tcaatctctt cgcagcattg atcgatgatg aacatcaccg
    14221 acggcacttc cagcataatg ccgagctgga tcttctcatc aaacggaatg tgttcgttac
    14281 gtagttgctg tttggcttct gccagttttt ctttcaccca taagatctct tccattgagg
    14341 agatcatcgg gatcatgatt ttcaggctgc cgtgagcgga ggcgcgcagg atcgagcgta
    14401 gttgtgtggt aaataacgac gcgtattctt cataaatacg cacggcgcga taaccgagga
    14461 acgggtttgc ctctgcggga atgtttagat aatcaacggg tttgtcgccg ccaatgtcca
    14521 tagtgcgcac aataatgctg cgtccgttgg cagactccag cgcctgacaa aaaatgttgt
    14581 acagctcgct ttcgcccggt gcactggtgc gatccatata gagcatttca gtgcggaaca
    14641 aaccaacgcc ttccgcacca ttgccgaatg cggcctgcgc ttccacggag tgagcgatgt
    14701 tagcggcaat ttcaatgcgg ataccgtcag cggtacgggc ttgttgggtc agccagacac
    14761 gctgttgctc acgcagggcg tcctgtacgc gggcttcttg ctgataataa cgagctacgg
    14821 cttctcctgg ctcaaccaca atcgccccgg cgttgccgtc gatataaatc gtttgatgct
    14881 gccacggagt gagggcatca atatccacac caaccagcgt cggaatgttg aacgaacggg
    14941 caaggatcac cgtatgtgag gtggtgccgc cacttttcag caacagacct ttgaggtgat
    15001 ttttatccag ttcgaggaac tggctggggg tcagttcatc agccatacaa atggcggact
    15061 gcgtcagttt gcccggtgcc gggaagcgtt gctcaccgta gatttgctgg agtaactgga
    15121 agcagacgtc gcgtacgtcc agggcacgtt cttgcaggta gctgctgctg gaacgggcga
    15181 actcttcgca aaagtgattc gcactggcga caatcgcttc ggcgcagctt aatccctcgc
    15241 tgacgcctgc cagtaagtgc tgacgtaaag atgtatcgcc agccagggat cggtgagctt
    15301 ccagtatcgc gctggtagca ccgtcgctat ccagcagacg aaactcaatg tttttcagta
    15361 ccagcgtcag gccgttttcc agtgtggatt gctcggcgtc aacgtctttg gctgcgggaa
    15421 gattactcag cgcattgaga tctaaagaag agatcggcgt cagaatgccg cctgcactac
    15481 cgctgcacac ggtgcgggcg cggataattt gcggattcag attggtcagt gaaaccggca
    15541 gtggttccag ttcgtcagat ttaacttccg ccagcggcgc atcgcagtgg gggaattcat
    15601 cgcgcagcca ttggcttaaa cgctggtgcg cttcctgttc gtcggccccg gaaatcagta
    15661 actggcagtt atcgcccgcc agcgtatcgg tgccaatcag cgcaagggcg cttttggcgt
    15721 tgcccttgcg gtcagtgtgc aggttatgcc actcaatttg tgatgaaaaa gtattacaca
    15781 gcgtttcaac gtggcttgcc ggacgcgcat gtacgccgtt aggtagctca caaataaatt
    15841 ccacaatcag ggccattgcc tctctcccgg tacggttttc tgtttacagg ataagagcgc
    15901 acggcaacgg cctgccatgt gacaaatctg ccaaaagctg gacaaatgta atgtaaccgt
    15961 caatttgcga cgcgtctcac aagacgccgt tttgcggcat gcttccggtt tatcgcaagt
    16021 tatgaggcgg atcgcatttt tgtactgata ttacaaaagt ccagtaaatg gcctttttat
    16081 ccactgtttg ctccgcctgc gattgcctat tgtttcctcc atcaaaaata tggacatggg
    16141 ctttcgcaag cccgggagca gcttatgaat gagttggtgc agatcctgaa aaatacccgt
    16201 cagcatttaa tgacgggcgt ttcacacatg attcccttcg tggtatcggg cggtattttg
    16261 ctggcggttt ccgtcatgtt gtatggcaaa ggcgcagtgc cggatgccgt agccgatcca
    16321 aatctgaaaa aactgtttga tatcggcgtt gcgggcttga cgctgatggt gcctttcctc
    16381 gccgcttaca ttggttattc cattgcagaa cgttctgcgc tggctccatg cgctatcggt
    16441 acctgggttg gtaacagctt tggtgcgggc ttctttggtg cactgatcgc cgggattatc
    16501 ggcggcatcg tggtgcatta cctgaagaaa attccggtgc ataaagttct gcgctcggtg
    16561 atgcctatct tcattattcc tatcgtcggc acactgatta ccgcaggcat catgatgtgg
    16621 ggcttgggcg agcctgtagg ggcgttgacc aacagcctga ctcagtggct tcaggggatg
    16681 cagcagggca gcattgttat gctggcggtg atcatgggtc tgatgctggc gttcgatatg
    16741 ggcggtccgg ttaacaaagt ggcctatgcc ttcatgctga tttgcgttgc tcagggtgtt
    16801 tataccgtgg tggccatcgc tgccgtgggt atttgtatcc cgccgctggg gatggggctg
    16861 gcgacgctga ttggtcgtaa aaatttctcc gcagaagagc gcgaaaccgg caaagcggca
    16921 ctggtgatgg gctgtgttgg cgttactgga ggggcgattc ctttcgccgc tgccgatccg
    16981 ctgcgtgtca ttccttccat catggtcggt tcagtttgtg gtgcagtaac tgcggcgctg
    17041 gtcggtgcgc agtgctatgc aggctggggt ggtctgattg tgctgccggt ggttgaaggc
    17101 aagctgggtt atatcgcagc agtggctgtc ggagcagtgg tgacggctgt ttgtgtgaac
    17161 gtgctgaaaa gtctggcgcg taaaaatggg tcttcgactg atgaaaaaga agacgacctg
    17221 gatttggatt ttgaaattaa ttaattgagg aacgagccat gacgaaaatt attgcagtaa
    17281 ccgcatgtcc ttcaggtgtt gcccatacct atatggcagc agaggcgctg gaaagtgccg
    17341 cgaaagcgaa aggctgggaa gtgaaagtag aaacctaggg gtcaattggt ctggaaaatg
    17401 aactgactgc ggaagatgtg gcgagcgctg atatggtgat tctgaccaaa gatatcggca
    17461 tcaagtttga agagcgtttt gctggcaaga ccatcgtgcg cgtcaacatc agcgatgcag
    17521 taaaacgtgc tgacgccatt atgagcaaaa ttgaagctca cctggcgcaa accgcttaag
    17581 ttcttctccc gctcgcaagg gcgggttcgc tttcccacag gagttcctca tgacgaatcg
    17641 tatctctcgc ctcaaaactg cactgtttgc caatacccgt gaaatctcgc tggagcgggc
    17701 gctgctttat accgccagcc atcggcaaac cgaaggcgaa ccggtgatat tgcgccgggc
    17761 gaaagcaaca gcgtatatcc ttgaacatgt tgaaatttcg attcgtgatg aagaactgat
    17821 tgccggtaac cgcaccgtaa aaccgcgcgc cgggattatg tcgccggaaa tggaccctta
    17881 ctggctgctg aaagagctgg atcaattccc gacgcgtccg caggaccgct ttgctatcag
    17941 cgaagaagat aaacgtatct accgtgaaga gttgttcccg tactgggaaa aacgttcgat
    18001 gaaagatttc atcaacgggc agatgacaga tgaagtaaaa gccgcgacca acacgcagat
    18061 tttcagcatc aaccagacgg ataaaggcca ggggcacatt attattgatt acccacgcct
    18121 gttgaatcac ggtctgggtg agctggtggc acagatgcag caacattgtc agcaacagcc
    18181 ggagaatcac ttttatcagg ccgcgttgtt actgctggaa gcctcgcaga aacacatttt
    18241 gcgttacgcc gaactggcgg aaacgatggc ggcaaactgc acagatgcac agcgtcgcga
    18301 agaactgctg actattgcgg agatctctcg ccataacgcc gaacataagc cgcagacgtt
    18361 ctggcaggcg tgccagttat tctggtacat gaacatcatt ctgcaatacg aatccaacgc
    18421 cagttcgctg tctttagggc gcttcgacca gtatatgttg ccgttctatc aggcatcttt
    18481 aacccagggc gaagatccgg cgttcctgaa agaactgctc gaatctttat gggtgaaatg
    18541 caacgacatc gtgctgttgc gctccaccag cagcgcgcgt tatttcgcag gtttcccgac
    18601 cggctatacc gcactgctcg gcgggttaac cgagaacgga cgtagcgcgg tgaacgtgct
    18661 ttcgttcctt tgccttgacg cctatcaaag cgtgcaatta ccgcaaccga acctcggcgt
    18721 gcgcactaac gccttgatcg acacgccgtt cctgatgaaa accgccgaaa ccattcgcct
    18781 cggcaccggt attccgcaaa tctttaacga tgaagtggtg gtaccagcgt tcctcaaccg
    18841 tggcgtttcg ctggaagatg cgcgcgacta ttccgtagtg ggctgtgtgg aattatctat
    18901 tcccggcaga acctacggct tgcatgacat cgcgatgttt aatctgctga aagtgatgga
    18961 aatctgcctg catgaaaatg aaggcaatgc tgcgctgact tatgaaggtt tactggagca
    19021 gatccgcgcc aagatcagcc actacatcac cctgatggtt gagggcagca atatttgtga
    19081 tatcggccat cgcgactggg cacctgtacc gctgctctcg tcttttatca gcgattgtct
    19141 ggaaaaaggt cgcgatatta ccgatggcgg cgcgcgttat aacttctccg gcgtacaggg
    19201 gatcggtatc gccaacctga gcgattctct ccatgcgttg aaagggatgg tttttgagca
    19261 acagcgttta agttttgacg aattgctgtc ggtattaaaa gccaactttg caacgccaga
    19321 aggcgaaaaa gtccgtgctc gcttaattaa ccgcttcgag aaatacggta acgatatcga
    19381 cgaggtggat aacatcagcg ccgaactgtt acgccactac tgcaaagaag tggaaaaata
    19441 ccagaacccg cgcgcggtta cttcacgccg ggatcgtata ccgtttctgc tcacgtcccg
    19501 ttgggatcgg tggttggcgc gacgccagac ggtcgttttg ccggggaaca actggcagac
    19561 ggcggcttgt cacctatgct gggccaggac gcacaagggc caacagcggt actgaagtca
    19621 gtcagtaagc tcgataatac gctgctgtct aacggtacgt tgctgaacgt gaaattcact
    19681 ccggcgaccc tggaaggtga agcgggatta cgcaaactgg ccgacttctt acgggcgttt
    19741 acccagctta agttgcagca tattcagttt aacgtggtga acgccgacac gttgcgggaa
    19801 gcgcaacagc gcccacaaga ttatgccggg ctggtggtgc gcgttgccgg atacagcgcc
    19861 ttctttgtcg aactgtcgaa ggagatccag gatgacatca tccgccggac agcgcatcag
    19921 ctgtaacgtt gtggaaacgc gccgcgatga tgtggcgcgc attttcaaca ttcagcgtta
    19981 ttcactgaat gacggcgagg gcattcgtac ggtggtcttt tttaaaggct gtccgcatct
    20041 ttgcccgtgg tgtgctaatc cggagtcgat ctccggcaaa atccagacgg tacgcagaga
    20101 ggcgaaatgt ctgcactgtg cgaaatgttt gcgtgatgcg gatgaatgcc cctccggggc
    20161 gtttgaacgg attggtcgcg atatcagcct tgacgctctg gaacgggaag tgatgaaaga
    20221 tgacattttc tttcgcacgt ccggcggcgg cgtcacgctt tctggcggcg aagtgttaat
    20281 gcaggcggag tttgctaccc gttttttaca gcgactgcgg ctgtggggtg tgtcatgcgc
    20341 cattgaaact gccggagacg cgccagcaag caagctgtta ccgctggcga aattgtgcga
    20401 tgaagtgctg ttcgatttaa aaattatgga cgcgactcag gcgcgggatg tggtgaagat
    20461 gaacctgcca cgcgtgctgg agaatctgcg tttgctggtg agtgagggtg tcaacgtgat
    20521 cccgcgttta ccgttgatcc ctggtttcac gctcagcagg gagaatatgc agcaggcgct
    20581 ggatgtactg atcccgctga aaatcaggca gatccatctg ttaccgtttc atcagtacgg
    20641 cgaaccgaaa taccgcctgc tggggaaaac atggtcgatg aaagaggtgc ctgcgccgtc
    20701 gtcagccgat gtgacaacga tgcgcgaaat ggcagaacgg gccggatttc aggttaccgt
    20761 gggaggttaa aatggcatac ctggtggcag taaccgcctg cgtaagcggc gtggcgcata
    20821 cttatatggc ggcggaacgg ctggaaaagt tgtgccagtt agagaagtgg ggcgtcagca
    20881 ttgaaactca gggcgcgctg ggaacggaga atcgtttagc ggacgaggat atccgtcggg
    20941 cggatgttgc tctgttgatt acggatatcg agcttgccgg tgccgagcga tttgaacatt
    21001 gccgctatgt gcaatgcagc atctacgcat tcctgcgtga gccgcagcgg gtaatgagcg
    21061 cggtgcgcaa agtgctttct gcgccgcagc aaacccatct tattctggag tagtcggttt
    21121 ttctgtcagt tggctgtggt actgccggcg atattccgac ggcgagcgtt cggtgttttt
    21181 acgaaacaga cggcagaagt agttgctgtc gacaaagccg caggcgtgcg ccacttcttt
    21241 taccttcagg tcatagcctt tcaataacgt cttagcgtgc tccagtcgcg tgtgattcag
    21301 gtattcgtta aaaccaatgg cccccgtttt ttgaaacagg tgcgagagat agtttggtga
    21361 aatatagaat gcctgtgcga cagattcgcg ggtaagcgcg gaggcatagc gttcgtcgat
    21421 ataatcgcga atagcttcaa atagtgcctg gctgcgtgag gcggtctgga tttggctgcc
    21481 gagcaaatcg cggcagtggc taagcaggct ggctacgata agccttgccg tttgctgctc
    21541 ctgcggctgc atttgcattt cattgagcgt ttgtagcaga aaagaaccta tacgtgggcc
    21601 gcgtcgggcg acgtgttgct tcgccagatt ttgatattgt ttgccatccc attgcacgac
    21661 gctgaagccg agttgctgtt tgccaaacag cacgctaaag gtggtagcgg gtgttttcca
    21721 ttgtgggaaa ttccagccac cagcggcaac gtacagcaca tcgccgggaa ctaacgttgc
    21781 tccagcgccg gtatcaacaa actcaccttc cagcacaatt tccagccgtg gaaaatcgac
    21841 ctgatacgca agatcgggca ccgggccgtt actgctggca aagtaaatct ggcgcaggga
    21901 cagcgggccg ttgatcaggc gggagagcag atagctgacg ccgtgataca tgttattccc
    21961 ttaggtatgt agacgggaaa acagtaactg gcattattaa aaaaggccac ctgaaaagtg
    22021 tggcctgagg ggagttcgat gtaaacattg cggcagttgt attgccggat gtggcgtaaa
    22081 cgccttatcc agcctaccgc cgcgatctgt aggccggata agacgcgtca agcgtcgcat
    22141 ccggcaaata gcgcctggct gataattact gattacctac ctggtcgcca tacggcagag
    22201 tgtcgtaatc gatcagcgcg tttttcttgt acgggttacc aatccagcgg gtagtttctt
    22261 tgaactgcgg attcaccact gaacgggttg gatcgtaacc gtcgtaagat aagcccgcca
    22321 aatctgacca ggtgtggatc agttccgcca ggctgtattt acgatcgaca tcctgtgaga
    22381 aatcacgggg atgagtcgct tgccattttt ccgacgtcca cagcaggaac ggaatggtgt
    22441 acatgtggcg cgtcgggttg tcttcgttac gcccctgagt tttgtgcggc ggcgtgtcgt
    22501 aaacctcttc accgtggtca gagaagtaaa ccaggaatcc gttcgggtct gccgctttaa
    22561 agtctttaat caggctggca accacatgat cgttatacag gttagcgttg tcataatcgt
    22621 tatatgattc cagctcttct gcgcttaatc ctggcggaac atgatcggta ttgccatcaa
    22681 acttgccctg attttccggg tagcggtatt tgtatttgat atgcgtaccc agcagatgaa
    22741 cgatgatcag tttcttcggc gcagggtcat tcagcacatc ctggaacggc ttcagcacgt
    22801 tggtgtcgta ttcacgcgca ctctgcgtac gttgctggtt catgtagtac tgcttgtcgg
    22861 tctggcgcga aaataccgtc agcatggtat tgcgggcggt catcgtctgc tggttggtga
    22921 tccagaaggt tttataaccc gcctgtttca tcatgttcat cagcgacggc tgcgtcagat
    22981 acagatccgg gttcttttca ttggcaaagg tcagcgcctg ttgcaggatt tcaatggtgt
    23041 acggacgaga cgtaactacg ttattaaaca cggtcagatt cggatcggtt ttatgcagcg
    23101 catccagctc cggcgtggtt tcacgcggat aaccgtacag actcatgcgt ccgcgctggg
    23161 tcgactcgcc aatcaccagc actaaagtgc gcggttcgtt acccgattca tctttgaaat
    23221 tagccagtgg cggcaaggca ttattttcat tcagtaactt tgttagcgag tttagttgct
    23281 gacgatactg ataatagccg gtcaggaatt gccacggtgc ggcaggctcc atacgcgagg
    23341 ccaggttatc caacgttttc tcaaacggct tgtttttgat aaacgtattc atggcgatcg
    23401 gatgcagaat caagccataa agcagggcaa aagagacaac ataacgccac ggctttggaa
    23461 tatagaccgg gcgcaggcgt gtccacagca gaactgccac cgccgtatag gccagcgcga
    23521 taagcacaat tttcaggctg aaatactggc ttaaatactc gctggcttcg ttggtgttgg
    23581 tttcgaacat cacaaacaga acgctctgcg agaactcctg cccgtagatg acgtagtagc
    23641 atagcgccgc cagagaggcc gcccatagca ccacgccgat tactgcggca ataattttaa
    23701 tccgcttcgg aaagaggaat accgggatca accacagcga gctgaataac agcgagtcgc
    23761 gaatgccgtt ggtgccacta taaccactga tgtaaataat ggcctgtagc agagtagaga
    23821 aaaaccaaaa gtagagcagt gcccaaccca gggctttcca gctaaaaagg ggtttagcct
    23881 ggacttctgt ggaatgcata gtaagaacct gtcttgaaaa aaatatcgcc gaatgtaacg
    23941 acaattcctt aaggatatct gaaggtatat tcagaatttg aataaaatgc agacagaaat
    24001 atattgaaaa cgagggtgtt agaacagaag tatttcagaa aaccctcgcg caaaagcacg
    24061 agggtttgca gaagaggaag attagccggt attacgcata cctgccgcaa tcccggcaat
    24121 agtgaccatt aacgcttgtt cgacgcgagg atccggttcc tggccttctt tttctgcctg
    24181 gcgggagcgg tgcagcaact cggcctgcaa tacgttcagc gggtcggtgt aaatattccg
    24241 tagctgaata gactctgcaa tccacggcag atcggccatc agatgggaat cgttggcaat
    24301 cgccagcacc actttgatgt cttcttcttg caggttgcgt aactctttac ctaacggcca
    24361 cagtgctttg tctaccagac gttgatcata gtattccgcc aaccacaggt ctgctttggc
    24421 gaagaccatc tccagcatgc cgagacgcgt cgagaagaat ggccaatcgc ggcacatggc
    24481 ttccagctcg ctctgtttgc catcttcgac cactttttgc agcgccgtac ctgcacccag
    24541 ccaggcgggg agcatcaggc ggttttgcgt ccaggcaaaa atccacggta tggcgcgcag
    24601 cgactcgacg ccgccggttg ggcgacgttt cgccggacgt gaacccaacg gcagtttgcc
    24661 caattcttgt tccggcgtag cggagcggaa gtaaggcaca aaatctttgt tttcacgtac
    24721 gtagccacgg tagagatcgc aggagatgac tgacagttca tccataatgc gacgccagct
    24781 ctctttcggc tcaggtggtg gcagcaggtt ggcttccaga atcgccccgg tgtaaagcga
    24841 caggctgctg acggtgattt ctggcagacc atatttaaag cggatcatct cgccctgttc
    24901 agtcacgcgc aggccacctt tcaggcttcc tggcggttgt gacagcagcg ccgcatgggc
    24961 aggtgcgccg ccgcgaccaa tggaaccgcc gcgaccgtgg aacaacgtca gctcaatacc
    25021 cgctttttcg caggttttga ttaatgcatc ctgtgcctga tattgcgccc aggaagctgc
    25081 catcacgccc gcatcttttg ctgagtcgga atagccaatc atcaccatct gtttgccctg
    25141 aatcaggccg cgataccagt cgatattgag cagctgggtc atgacatcgt tggcgttgtt
    25201 caggtcatcg agggtttcaa acagcggagc aaccggcatc gcaaacccga tacccgcttc
    25261 tttcagcagc aggtggacag ccagtacgtc ggacggcgtt ttcgccatcg agatcacgta
    25321 ggcggcaatg gagccttgcg gcgcttcggc aatcacctgg caggtatcga gcacttcgcg
    25381 cgtttcggcg cttggttgcc agttgcgcgg cagaagcgga cgtttggagt tcagttcgcg
    25441 gatcaggaac gcctgtttgt cggcctctga ccagctttcg tagtcgccga taccgaggta
    25501 gcgggtcagc tcgcccagcg cttcggtatg gcgggtgctc tcctgacgga tatcaatacg
    25561 gaccagcggt acgccgaaac atttcacgcg gcgcagggtg tcgagcagat cgccgttggc
    25621 gataataccc atgccacacg cctgaagtga ctggtagcaa gcgtagagcg gttcccacag
    25681 ttcttcgttt tgtgtcagca gcccttctgg ttttggcagt tcttcgcctt tcaggcgcgc
    25741 ttccagccat gcctgtgtcg ccatcaggcg agaacgcagg tttttcatca gatagcgata
    25801 cggttctgcg gcaccttctt cgccaaccag cgccagcagt tcaggggtcg cttcaaccat
    25861 cgacagttca gaaaccagaa cctgaatatc tttcaggaac agatcggtgg ctttccagcg
    25921 actgagtagc aggacgtggc gggtgatatc ggcagtgacg ttcgggttgc cgtcgcggtc
    25981 accgcccatc cacgaagtaa aacggaccgg aacaaattcg acgggcagtt tgtagccgag
    26041 gttctcttcc agttgttcgt tcagttcgcg caagtaattc ggtacgcctt gccacaggct
    26101 gttttctact acggcaaagc cccatttggc ttcatctacc gggcttggac gcagcttacg
    26161 gatttcatca gtatgccatg actgggcgat taactggcgc aggcgacgca tcagctggtt
    26221 gtgttcgtag tcagcgatat ctttgttatc gagctgtttt aagcaggcgt tcacttccac
    26281 cattttgtgg atcagtgtac gacgggtaat ttcggttggg tgagccgtga ggaccagttc
    26341 cagcgacagc gattccactg cttttttgat ggtgtcttcg ctcagttccg gctggttttt
    26401 cagtttgcgc agggtgcggg cgatcacttc cgggttgctg gcagcttcgc ctttcggcga
    26461 aatgctgtgg tattgctcgg cggtgttggc caggttcagg aactgactaa acgcacgcgc
    26521 aacgggcagc agctcgtcgt tcgacaaatt ttgtaaggtg gtgagcaact cctggcggtt
    26581 agcatcattg ccagcgcgtg aagatttcga caacttacgg atagtttcta cgcgttcaag
    26641 aatgtgttct cccaacgcat ccttgatggt ttctcccagc actttgccga gcatactgac
    26701 attactacgc aatgcggaat attgttcgtt catattaccc cagacacccc atcttatcgt
    26761 ttgatagccc tgtatccttc acgtcgcatt ggcgcgaata tgctcgggct ttgcttttcg
    26821 tcgtctttta taaagccacg taaaagcggt gacgtcaaat gctgcgaaat cgcttcagca
    26881 aacgaataaa tagcaggaat ttacgccatt aaattcacga cgctttaaat aagcgtaact
    26941 tatggaaatg ttaaaaaatc gccatacata acaccaaagg tgtaggtcgg ataagatgcg
    27001 caagcatcgc atccgacatt attgcggcac cggagtttgg caacggtgcc ggatgcggcg
    27061 cgagcgcctt atccggccta cgttttaatg ccagcaaaaa tggtgaatta cctggataat
    27121 caactcacgg gtgggtttaa taaaccgcgt ctccagatat tcatcaggct gatgagcttg
    27181 attgatggaa cccggaccga gcaccagcgt cgggcataac gtttgaataa acggcgcttc
    27241 ggtacagtaa ttcaccactt cggtttttgc tccgagcaat ttctcaacca cttcaaccag
    27301 ttgatgattc ggtgggcatt catagccagg gatcggcgga tgcagctcgt cgaccgtcag
    27361 acgacccggc cagcgttcgc tcaccggagc caatgcatcg ttgagcaaac cattaagttc
    27421 attgagtgtc atgctaggca gcggacgaat atccatatgc aactcacagc aagcgcaaat
    27481 acggttagaa gcgtcgccac cgtgaatatg cccgaggttg agcgtagggt atggcacggt
    27541 aaatgcttcg tagtgataac gttctttcag gttatcgcgc aattgcaaaa tatgcccgat
    27601 ggcgtcgtgc attagttcga tagcgttaac tccgcgtgct ggatcgctgg agtgccccga
    27661 ctggccctga atacggatgg cgttagagat atgaccttta tgtgcgcgta ccggttgtag
    27721 tgacgtcggt tcgccaatga tggcgcaatc cgggcgcagg gcggtagttt cggcaaaata
    27781 acgcgctccg gccatactgg tttcttcatc agcagtcgcc agaatgtaga gcggtttttt
    27841 cagtttcgtg acgtcgacat cgcgtagcgt atcaaggata aacgcaaaaa agcctttcat
    27901 gtcggcggtg cctaagccgt aaagcttgcc gtcatgctcc gtcagtgtaa acggatcgcg
    27961 cgtccagcga ccgtcatcaa atggcaccgt atcggtatgc cccgccagca acaagccgcc
    28021 agccccctgt ccgatacttg ccagcatatt gaatttgttg cgagttcctg gcacaggctg
    28081 cacttccaca ttgaagccta aatctttaaa ccagtccgcc agcagagtga ttaaatctgc
    28141 attgctttga tcgagtgcct cttccgtggc gcttattgaa ggtgtggcaa tcagagcacg
    28201 gtaaatctcg ataaatggcg gtaatttgtt tttcattgtt gacacacctc tggtcatgat
    28261 agtatcaata ttcatgcagt atttatgaat aaaaatacac taacgttgag cgtaataaaa
    28321 cccaccagcc gtaaggtgaa tgttttacgt ttaacctggc aaccagacat aagaaggtga
    28381 atagccccga tgttgaatac gctgattgtg ggtgccagcg gctacgctgg cgcagagcta
    28441 gtgacctatg taaatcgcca tccgcatatg aacataaccg ctttgactgt ttcagcgcaa
    28501 agcaatgatg cgggaaagtt aatctccgat ttgcatccgc agctaaaagg catcgttgag
    28561 ctgccgttgc agccgatgtc ggatatcagc gagtttagcc caggggtgga cgtagtgttt
    28621 ctcgccaccg cccatgaagt tagccacgat ttagcgccgc agtttcttga agcgggctgc
    28681 gtggtgttcg acctttccgg tgcgtttcgt gttaacgacg ccaccttcta tgaaaaatat
    28741 tacggcttta cccatcaata cccggaactg ctggaacagg cagcctacgg tctggcggag
    28801 tggtgcggta ataaattaaa agaagcgaat ttgattgcgg tgccaggctg ttatccgacg
    28861 gcggcacagc tggcgctgaa accgttgatt gatgccgatc ttcttgacct caatcagtgg
    28921 ccggtgatca acgccaccag cggcgtgagc ggtgcaggac gtaaggcagc gatttcaaat
    28981 agtttttgtg aagttagcct gcaaccgtat ggcgtcttta ctcatcgcca tcaaccagag
    29041 atcgccacac acctcggtgc tgacgttatc ttcaccccac atctgggcaa tttcccgcgc
    29101 ggcattctcg aaaccattac ctgccgcctg aaatcgggtg tgacccaggc gcaagtcgcg
    29161 caagtgttac agcaggcgta tgcccataaa ccgctggtgc ggctgtatga caaaggcgtt
    29221 ccggcgctga aaaatgtcgt tgggctgcca ttttgcgata tcgggtttgc cgttcagggc
    29281 gagcatctga ttattgtggc gaccgaagac aacttactga aaggcgcggc ggcacaagcg
    29341 gtacagtgcg ccaatattcg tttcggctat gcggaaacgc agtctcttat ttaagggtgc
    29401 aatgatgaat ccattaatta tcaaactggg cggcgtactg ctggatagtg aagaggcgct
    29461 ggaacgtctg tttagcacac tggtgaatta tcgtgagtca catcagcgtc cgctggtgat
    29521 tgtgcacggc ggcggttgcg tggtggatga gctgatgaaa gggctgaatc tgccggtgaa
    29581 aaagaaaaac ggcctgcggg tgacgcctgc tgatcagata gacattatca ccggagcact
    29641 ggcgggaacg gcaaataaaa ccctgttggc atgggcgaag aaacatcaga ttgcggccgt
    29701 aggtttgttt ctcggtgacg gcgacagcgt caaagtgacc cagcttgatg aagagttagg
    29761 tcatgttgga ctggcgcagc caggttcgcc taagcttatc aactccttgc tggagaacgg
    29821 ttatctgccg gtggtcagct ccattggcgt aacagacgaa gggcaactga tgaacgtcaa
    29881 tgccgaccag gcggcaacgg cgctggcggc aacgctgggc gcggatctga ttttgctctc
    29941 cgacgtcagc ggcattctcg acggcaaagg gcaacgcatt gccgaaatga ccgccgcgaa
    30001 agcagaacaa ctgattgagc agggcattat tactgacggc atgatagtga aagtgaacgc
    30061 ggcgctggat gcggcccgca cgctgggccg tccggtagat atcgcctcct ggcgtcatgc
    30121 ggagcagctt ccggcactgt ttaacggtat gccgatgggt acgcggattt tagcttaagt
    30181 tttgttggcc ggaggcgcag ctttccggca ttgaatttca aaataaggaa acagagttat
    30241 ggcactttgg ggcgggcgtt ttacccaggc agcagatcaa cggttcaaac aattcaacga
    30301 ctcactgcgc tttgattacc gtctggcgga gcaggatatt gttggctctg tggcctggtc
    30361 caaagccctg gtcacggtag gcgtgttaac cgcagaagag caggcgcaac tggaagaggc
    30421 gctgagcgtg ctgctggaag atgttcgcgc caggccacaa caaatccttg aaagcgacgc
    30481 cgaagatatc catagctggg tggaaggcaa actgatcgac aaagtgggcc agttaggcaa
    30541 aaagctgcat accgggcgta gtcgtaacga tcaggtagcg actgacctga aactgtggtg
    30601 caaagatacc gttagcgagt tactgacggc taaccgacag ctgcaatcgg cgctggtgga
    30661 aaccgcacaa aacaatcagg acgcggtcat gccaggttac actcacctgc aacgcgccca
    30721 gccggtgacg ttcgcgcact ggtgcctggc ctatgttgag atgctggcgc gtgatgaaag
    30781 ccgtttgcag gatgcgctta agcgtctgga tgtcagccca ttaggctgtg gcgcgctggc
    30841 gggaacggcc tatgaaatcg accgtgaaca gttagcaggc tggctgggct ttgcttcggc
    30901 gacccgtaac agtctggaca gcgtttctga ccgtgaccat gtgttggaac tgctgtcggc
    30961 tgccgctatc ggcatggtgc atctgtcgcg ttttgctgaa gatctgattt tctttaacac
    31021 cggcgaagcg gggtttgtgg agctttctga ccgcgtgact tccggttcat cattaatgcc
    31081 gcagaagaaa aacccggatg cgctggagct gattcgcggt aaatgcggtc gggtgcaggg
    31141 tgcgttaacc ggcatgatga tgacgctgaa aggtttgccg ctggcttaca acaaagatat
    31201 gcaggaagac aaagaaggtc tgttcgacgc gctcgatacc tggctggact gcctgcatat
    31261 ggcggcgctg gtgctggacg gcattcaggt gaaacgtcca cgttgccagg aagcggctca
    31321 gcagggttac gccaacgcca ccgaactggc ggattacctg gtggcgaaag gcgtaccttt
    31381 ccgcgaggcg caccatatcg tgggtgaagc ggtggtggaa gccattcgtc agggcaaacc
    31441 gctggaagat ctgccgctcg acgagttgca gaaattcagc ccggtgattg gcgaagatgt
    31501 ctatccgatt ttgtcgctgc aatcgtgcct cgacaagcgt gcggcaaaag gcggcgtctc
    31561 accgcagcag gtggcccaag cgattgcttt tgcgcaggcg cggttggggt aagaacattt
    31621 atatgtacaa atttgagcct ggcttatcgc cgggcttttt tatggcaaaa aaaagcggat
    31681 cctggagatc cgcaaaagtt cacgttggct ttagttattc gagttgagaa actctcgaaa
    31741 cgggcagtga cttcaagggt taaaagaggt gccgctccgt ttctgtgagc aattatcagt
    31801 cagaatgctt gatagggata atcgttcatt gctattctac ctatcgccat gaactatcgt
    31861 ggcgatggag gatggataat gaatattcgt gatcttgagt acctggtggc attggctgaa
    31921 caccgccatt ttcggcgtgc ggcagattcc tgccacgtta gccagccgac gcttagcggg
    31981 caaattcgta agctggaaga tgagctgggc gtgatgttgc tggagcggac cagccgtaaa
    32041 gtgttgttca cccaggcggg aatgctgctg gtggatcagg cgcgtaccgt gctgcgtgag
    32101 gtgaaagtcc ttaaagagat ggcaagccag cagggcgaga cgatgtccgg accgctgcat
    32161 attggtttga ttcccacggt tggaccgtac ctgctaccgc atattatccc gatgctgcac
    32221 cagacctttc caaagctgga aatgtatctg catgaagcac agacccacca gttactggcg
    32281 caactggaca gtggcaaact cgattgcgtg atcctcgcgc tggtgaaaga gagcgaagca
    32341 ttcattgaag tgccgttgtt tgatgagcca atgttgctgg ctatctatga agatcacccg
    32401 tgggcgaacc gcgaatgcgt accgatagcc gatctggcag gggaaaaact gctgatgctg
    32461 gaagatggtc actgtttgcg cgatcaggca atgggtttct gttttgaagc cggggcggat
    32521 gaagatacac acttccgcgc gaccagcctg gagacgctgc gcaacatggt ggcggcaggt
    32581 agcgggatca ctttactgcc agcgctggct gtgccgccgg agcgcaaacg cgatggggtt
    32641 gtttatctgc cgtgcattaa gccggaacca cgccgcacta ttggcctggt ttatcgtcct
    32701 ggctcaccgc tgcgcagccg ctatgagcag ctggcagagg ccatccgagc aagaatggat
    32761 ggctatttcg ataaagtgtt aaaacaggcg gtttaaaccg tttaacgcag ctacccgata
    32821 ggcttccgcc atggtcgggt agttaaaggt ggtgttgacg aagtactcaa tagtgttgcc
    32881 gccacctttc tgttccataa tcgcctgacc gatatgaata atttcggcag cgcgctcgcc
    32941 aaagcagtga atacccagaa tctcttttgt ttcccgatgg aacaaaattt tcagcgtgcc
    33001 cacgttcatg ccgacgattt gtgcgcgtgc cagatgttta aactgggcgc ggcccacttc
    33061 atatggcact ttcatcgcgg tcagctgctg ttcggttttg cccacagagc tgatttccgg
    33121 gatggtgtaa atgccggtag ggatatcttc aatcagatgc gcggtggctt cgccttttac
    33181 cagcgcctgc gcggcaatgc gcccctggtc ataggccgct gatgccaggc tcggataacc
    33241 aatcacgtcg cccaccgcgt aaacgtgcgg ctgtgcggtc tgatacatgc tgttgacctt
    33301 cagctgtccg cgactgtcag tttccagccc aatgttctgt aacgccagcg aatcggtatt
    33361 accggtacga ccgttggcat agagcaggca gtcagctttc agttttttac ccgacttcag
    33421 atgcatgatc acaccgtcgt cacagccttc gatcttctcg tactcttcgt tgtgacgaat
    33481 cactacgcca ctgttccaga agtgatagga gagagaatct gacatctctt gatcgagaaa
    33541 tgccagcagg cgatcgcggg tgttgatcag atccactttt acatccatac cgcggaagat
    33601 cgacgcatat tcacagccga tcactccagc accatagata agtacatggc gcggttcgtg
    33661 gtgcatgcta agaattgagt cgctgtcgta aatgcgtgga tgggtgaaat caacatctgt
    33721 tggatgatat ggacgagagc cgcaggcaat aacaaatttt tcagcggtta gtgtttcgac
    33781 gctgccgtcc gggcaatcca gcgccaacgt atgctcgtca acaaagcgag cgttcccctg
    33841 caatatttca cagtgattac gttcgtaaaa tccctgacgc atgcgcgttt gttgattaat
    33901 cacgttatcg gcatggttaa ggatatcggc aaaagaagag cggagcagtc gcgaatggtc
    33961 gctgtatagt gggttttgat tgaattctat aatgcggctg acggcgtgac ggagagcttt
    34021 cgacgggatg gtgccccagt gggtgcaacc gccgccaaca ttttgataac gctcgataac
    34081 tgcgacgcgc acaccttgct taaccaggcc cattgcagcg ccttcgccgc cggggccgga
    34141 acctattact atggcatcgt aatcgtagga atgtggcatg gtagggctta cctgttctta
    34201 tacataaaag caacagaatg gtaacattct atcgcgggta agccaattga tccccgtcat
    34261 ttatctggct atatcctgag cggcctttgc tttgtctgtt tcttactttt gccctgacgt
    34321 tttattggat ttttatcgac gatactctcc gtttaagcgg caggtttccg ctgtacgtaa
    34381 aagaaccggc cagtaaatat gttttattgc gttaccgttc attcacaata ctggagcaat
    34441 ccagtatgtt cattctctgg tatagtgcca gcagtacttt tggcaaggat tcagacatcg
    34501 tgatgggcat aagagcgcaa caaaaagaaa aaacccgccg ttcgctggtg gaagccgcat
    34561 ttagccaatt aagtgctgaa cgcagcttcg ccagcctgag tttgcgtgaa gtggcgcgtg
    34621 aagcgggcat tgctcccacc tctttttatc ggcatttccg cgacgtagac gaactgggtc
    34681 tgaccatggt tgatgagagc ggtttaatgc tacgccaact catgcgccag gcgcgtcagc
    34741 gtatcgccaa aggcgggagt gtgatccgca cctcggtctc cacatttatg gagttcatcg
    34801 gtaataatcc taacgccttc cggttattat tgcgggaacg ctccggcacc tccgctgcgt
    34861 ttcgtgccgc cgttgcgcgt gaaattcagc acttcattgc ggaacttgcg gactatctgg
    34921 aactcgaaaa ccatatgccg cgtgcgttta ctgaagcgca agccgaagca atggtgacaa
    34981 ttgtcttcag tgcgggtgcc gaggcgttgg acgtcggcgt cgaacaacgt cggcaattag
    35041 aagagcgact ggtactgcaa ctgcgaatga tttcgaaagg ggcttattac tggtatcgcc
    35101 gtgaacaaga gaaaaccgca attattccgg gaaatgtgaa ggacgagtaa tgaaacaagc
    35161 aaatcaagat agaggtacgc tgctgctggc gttagttgct ggcttatcga ttaatggtac
    35221 tttcgcagcg ctgtttagct ccattgtgcc attttctgta ttcccgatta tttccctggt
    35281 gctgacggtt tactgcctgc atcaacgtta tcttaatcgc actatgccgg taggcttgcc
    35341 gggtctggca gctgcctgtt ttattctcgg cgtactgctg tacagcacgg tagttcgtgc
    35401 ggaatatccg gatatcggct ctaacttctt cccggcagta ctctccgtca ttatggtgtt
    35461 ctggattggc gcgaagatgc gtaaccgtaa gcaggaagtt gctgagtaat cggtattatg
    35521 ccggatgctg gcgcatccgg catgggtttt acttcgcggt cagtaatacg ccgcactcca
    35581 tatggtgcgt gtaggggaac tgatcaaaca gagccagacg ttcgaccttg tgcgtctggc
    35641 ttaatgtttc cagattcttg cataacgttt ccgggttaca ggagatgtac aaaatacacg
    35701 gatacgcctg caccattttc tcggtttcac tgtccagacc gctgcgcggt gggtcgacaa
    35761 aaatggtttc gcactgataa ctctttaagt cgatcccttg caggcggtta aactcgcgca
    35821 caccattcat cgcctgagta aattcttctg ccgccatacg aataatttgt acgttatcaa
    35881 tatggttagc tgcgatgttg tattgcgcag cagcaaccga cggcttagcg atttcggtgg
    35941 ctaatacccg atcaaaattg cgcgccagcg ctaatgaaaa gttaccgttg ccgcagtaca
    36001 gctccagtaa atcgcctttt gagcctttgg ttacgtccag cgcccattcc agcatctgaa
    36061 tattcatcgc cgcgttcggc tgggtaaagc tgttttctac ctgacggtag atcatctctt
    36121 tccctgcgac cggcagacgc tcgtcgatgt aatcttgatc cagctcgatt ttggttttcg
    36181 ttgcccgacc aatcagatgc acattcagat tctgcgcgcg cagtgcatcg cgcagggcct
    36241 ccgcttcctg acgccactca tcatccagct tcttatggta tagcagggaa accaccgcct
    36301 gattactcag cgtggtgagg taatcaatct ggaacaactt gtggcgcaga acgggattat
    36361 tacgcacacc cgcaatcatc gccgtcatca actggttgat aagttcactg gcggcgggga
    36421 agctatccac gcggatacgg cttttggttt gttgatcgaa aatgatgtga tacaggtcat
    36481 cgccatcgtg ccagatgcgg aactccgcac gcatccggta atgactgacc ggcgagcgaa
    36541 acacttccgg aaccaggtca gaaaacggtg ccatcatact ttgcaaacgt accacttttt
    36601 cggctaactg cgcttcatac tgttctgttg gaaggtgttc gggggtcatg atgtatcctg
    36661 aaaaattaaa gtacgcggcg attgtaggga ttgctcatca gatgtccaga tcttgatgaa
    36721 ttcctatttg tgagctacgt ctggacagta acttgttaca acctgtagca tccacttgtc
    36781 ggtcctgtga gttaataggg aatccagtgc gaatctggag ctgacgcgca gcggtaagga
    36841 aaggtgcgat gattgcgtta tgcggacact gccatccggt gggaagtcat catctcttag
    36901 tatcttagat acccctccaa gcccgaagac ctgccggcca acgtcgcatc tggttctcat
    36961 catcgcgtaa tattgatgaa acctgcggca tccttcttct attgtggatg ctttacaatg
    37021 attaaaaaag cttcgctgct gacggcgtgt tctgtcacag ccttttccgc ttgggcacag
    37081 gataccagcc cggatactct cgtcgttact gctaaccgtt ttgaacagcc gcgcagcact
    37141 gtgcttgcac caaccaccgt tgtgacccgt caggatatcg accgctggca gtcgacctcg
    37201 gtcaatgatg tgctgcgccg tcttccgggc gtcgatatca cccaaaacgg cggttcaggt
    37261 cagctctcat ctatttttat tcgcggtaca aatgccagtc atgtgttggt gttaattgat
    37321 ggcgtacgcc tgaatctggc gggggtgagt ggttctgccg accttagcca gttccctatt
    37381 gcgcttgtcc agcgtgttga atatatccgt gggccgcgct ccgctgttta tggttccgat
    37441 gcaataggcg gggtggtgaa tatcatcacg acgcgcgatg aacccggaac ggaaatttca
    37501 gcagggtggg gaagcaatag ttatcagaac tatgatgtct ctacgcagca acaactgggg
    37561 gataagacac ggggaacgct gttgggcgat tatgcccata ctcatggtta tgatgttgtt
    37621 gcctatggta atacaggaac gcaagcgcag acagataacg atggtttttt aagtaaaacg
    37681 ctttatggcg cgctggagca taactttact gatgcctgga gcggctttgt gcgcggctat
    37741 ggctatgata accgtaccaa ttatgacgcg tattattctc ccggttcacc gttgctcgat
    37801 acccgtaaac tctatagcca aagttgggac gccgggctgc gctataacgg cgaactgatt
    37861 aaatcacaac tcattaccag ctatagccat agcaaagatt acaactacga tccccattat
    37921 ggtcgttatg attcgtcggc gacgctcgat gagatgaagc aatacaccgt ccagtgggca
    37981 aacaatgtca tcgttggtca cggtagtatt ggtgcgggtg tcgactggca gaaacagact
    38041 acgacgccgg gtacaggtta tgttgaggat ggatatgatc aacgtaatac cggcatctat
    38101 ctgaccgggc tgcaacaagt cggcgatttt acctttgaag gcgcagcacg cagtgacgat
    38161 aactcacagt ttggtcgtca tggaacctgg caaaccagcg ccggttggga attcatcgaa
    38221 ggttatcgct tcattgcttc ctacgggaca tcttataagg caccaaatct ggggcaactg
    38281 tatggcttct acggaaatcc gaatctggac ccggagaaaa gcaaacagtg ggaaggcgcg
    38341 tttgaaggct taaccgctgg ggtgaactgg cgtatttccg gatatcgtaa cgatgtcagt
    38401 gacttgatcg attatgatga tcacaccctg aaatattaca acgaagggaa agcgcggatt
    38461 aagggcgtcg aggcgaccgc caattttgat accggaccac tgacgcatac tgtgagttat
    38521 gattatgtcg atgcgcgcaa tgcgattacc gacacgccgt tgttacgccg tgctaaacag
    38581 caggtgaaat accagctcga ctggcagttg tatgacttcg actggggtat tacttatcag
    38641 tatttaggca ctcgctatga taaggattac tcatcttatc cttatcaaac cgttaaaatg
    38701 ggcggtgtga gcttgtggga tcttgcggtt gcgtatccgg tcacctctca cctgacagtt
    38761 cgtggtaaaa tagccaacct gttcgacaaa gattatgaga cagtctatgg ctaccaaact
    38821 gcaggacggg aatacacctt gtctggcagc tacaccttct gaaccacgtc ccaccgtgct
    38881 ggtgtttgac tccggcgtcg gtgggttgtc ggtctatgac gagatccggc atctcttacc
    38941 ggatctccat tacatttatg ctttcgataa cgtcgctttc ccgtatggcg aaaaaagcga
    39001 agcgtttatt gttgagcgag tggtggcaat tgtcaccgcg gtgcaagaac gttatcccct
    39061 tgcgctggct gtggtcgctt gcaacactgc cagtaccgtt tcacttcctg cattacgcga
    39121 aaagttcgac ttcccggttg ttggtgtcgt gccggcgatt aaacctgctg cacgtctgac
    39181 ggcaaatggc attgtcggat tactggcaac ccgcggaaca gttaaacgtt cttatactca
    39241 tgagctgatc gcgcgtttcg ctaatgaatg ccagatagaa atgctgggct cggcagagat
    39301 ggttgagttg gctgaagcga agctacatgg cgaagatgtt tctctggatg cactaaaacg
    39361 tatcctacgc ccgtggttaa gaatgaaaga gccgccagat accgttgtat tgggttgcac
    39421 ccatttccct ctactacaag aagaactgtt acaagtgctg ccagagggaa cccggctggt
    39481 ggattctggc gcagcgattg ctcgccgaac ggcctggttg ttagaacatg aagccccgga
    39541 tgcaaaatct gccgatgcga atattgcctt ttgtatggca atgacgccag aagctgaaca
    39601 attattgccc gttttacagc gttacggctt cgaaacgctc gaaaaactgg cagttttagg
    39661 ctgatttggt tgaatgttgc gcggtcagaa aattatttta aatttcctct tgtcaggccg
    39721 gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac gccgccgggt
    39781 cagcggggtt ctcctgagaa tctcaacaga gaaaagcaaa
//
LOCUS       DAZPJR010000016        39748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPJR010000016 DAZPJR010000000
VERSION     DAZPJR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.017"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..268)
                     /locus_tag="ACHVB7_000834"
     CDS             complement(<1..268)
                     /locus_tag="ACHVB7_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1912901.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPV"
     gene            complement(428..640)
                     /gene="rpmE"
                     /locus_tag="ACHVB7_000835"
     CDS             complement(428..640)
                     /gene="rpmE"
                     /locus_tag="ACHVB7_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR1912902.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            843..3041
                     /gene="priA"
                     /locus_tag="ACHVB7_000836"
     CDS             843..3041
                     /gene="priA"
                     /locus_tag="ACHVB7_000836"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR1912903.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3197..4222
                     /gene="cytR"
                     /locus_tag="ACHVB7_000837"
     CDS             3197..4222
                     /gene="cytR"
                     /locus_tag="ACHVB7_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR1912904.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4314..5273
                     /gene="ftsN"
                     /locus_tag="ACHVB7_000838"
     CDS             4314..5273
                     /gene="ftsN"
                     /locus_tag="ACHVB7_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR1912905.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5366..5896
                     /gene="hslV"
                     /locus_tag="ACHVB7_000839"
     CDS             5366..5896
                     /gene="hslV"
                     /locus_tag="ACHVB7_000839"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR1912906.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5906..7237
                     /gene="hslU"
                     /locus_tag="ACHVB7_000840"
     CDS             5906..7237
                     /gene="hslU"
                     /locus_tag="ACHVB7_000840"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR1912907.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7304..8230
                     /gene="menA"
                     /locus_tag="ACHVB7_000841"
     CDS             7304..8230
                     /gene="menA"
                     /locus_tag="ACHVB7_000841"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR1912908.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8323..8808
                     /gene="rraA"
                     /locus_tag="ACHVB7_000842"
     CDS             8323..8808
                     /gene="rraA"
                     /locus_tag="ACHVB7_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR1912909.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8893..9138)
                     /gene="zapB"
                     /locus_tag="ACHVB7_000843"
     CDS             complement(8893..9138)
                     /gene="zapB"
                     /locus_tag="ACHVB7_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR1912910.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9564..10409
                     /gene="glpF"
                     /locus_tag="ACHVB7_000844"
     CDS             9564..10409
                     /gene="glpF"
                     /locus_tag="ACHVB7_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR1912911.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10432..11943
                     /gene="glpK"
                     /locus_tag="ACHVB7_000845"
     CDS             10432..11943
                     /gene="glpK"
                     /locus_tag="ACHVB7_000845"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR1912912.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            12073..13083
                     /gene="glpX"
                     /locus_tag="ACHVB7_000846"
     CDS             12073..13083
                     /gene="glpX"
                     /locus_tag="ACHVB7_000846"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1912913.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13180..13926
                     /gene="fpr"
                     /locus_tag="ACHVB7_000847"
     CDS             13180..13926
                     /gene="fpr"
                     /locus_tag="ACHVB7_000847"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR1912914.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13931..14359)
                     /gene="uspD"
                     /locus_tag="ACHVB7_000848"
     CDS             complement(13931..14359)
                     /gene="uspD"
                     /locus_tag="ACHVB7_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR1912915.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14386..14685)
                     /locus_tag="ACHVB7_000849"
     CDS             complement(14386..14685)
                     /locus_tag="ACHVB7_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR1912916.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14897..15337)
                     /locus_tag="ACHVB7_000850"
     CDS             complement(14897..15337)
                     /locus_tag="ACHVB7_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1912917.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15438..16037
                     /locus_tag="ACHVB7_000851"
     CDS             15438..16037
                     /locus_tag="ACHVB7_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR1912918.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16145..16912
                     /gene="tpiA"
                     /locus_tag="ACHVB7_000852"
     CDS             16145..16912
                     /gene="tpiA"
                     /locus_tag="ACHVB7_000852"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR1912919.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16967..17722)
                     /gene="cdh"
                     /locus_tag="ACHVB7_000853"
     CDS             complement(16967..17722)
                     /gene="cdh"
                     /locus_tag="ACHVB7_000853"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR1912920.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17829..18818)
                     /gene="sbp"
                     /locus_tag="ACHVB7_000854"
     CDS             complement(17829..18818)
                     /gene="sbp"
                     /locus_tag="ACHVB7_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR1912921.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19138..20100)
                     /gene="pfkA"
                     /locus_tag="ACHVB7_000855"
     CDS             complement(19138..20100)
                     /gene="pfkA"
                     /locus_tag="ACHVB7_000855"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR1912922.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20281..21183)
                     /gene="fieF"
                     /locus_tag="ACHVB7_000856"
     CDS             complement(20281..21183)
                     /gene="fieF"
                     /locus_tag="ACHVB7_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR1912923.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21331..21831)
                     /gene="cpxP"
                     /locus_tag="ACHVB7_000857"
     CDS             complement(21331..21831)
                     /gene="cpxP"
                     /locus_tag="ACHVB7_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR1912924.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21981..22679
                     /gene="cpxR"
                     /locus_tag="ACHVB7_000858"
     CDS             21981..22679
                     /gene="cpxR"
                     /locus_tag="ACHVB7_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR1912925.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22676..24049
                     /gene="cpxA"
                     /locus_tag="ACHVB7_000859"
     CDS             22676..24049
                     /gene="cpxA"
                     /locus_tag="ACHVB7_000859"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR1912926.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24155..24829)
                     /gene="yiiM"
                     /locus_tag="ACHVB7_000860"
     CDS             complement(24155..24829)
                     /gene="yiiM"
                     /locus_tag="ACHVB7_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR1912927.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24978..25961)
                     /gene="kdgT"
                     /locus_tag="ACHVB7_000861"
     CDS             complement(24978..25961)
                     /gene="kdgT"
                     /locus_tag="ACHVB7_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR1912928.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26221..26841)
                     /gene="sodA"
                     /locus_tag="ACHVB7_000862"
     CDS             complement(26221..26841)
                     /gene="sodA"
                     /locus_tag="ACHVB7_000862"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR1912929.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27126..28160
                     /gene="rhaT"
                     /locus_tag="ACHVB7_000863"
     CDS             27126..28160
                     /gene="rhaT"
                     /locus_tag="ACHVB7_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR1912930.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28157..29005)
                     /gene="rhaR"
                     /locus_tag="ACHVB7_000864"
                     /pseudo
     CDS             complement(28157..29005)
                     /gene="rhaR"
                     /locus_tag="ACHVB7_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(29079..29915)
                     /gene="rhaS"
                     /locus_tag="ACHVB7_000865"
     CDS             complement(29079..29915)
                     /gene="rhaS"
                     /locus_tag="ACHVB7_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR1912931.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30203..31672
                     /gene="rhaB"
                     /locus_tag="ACHVB7_000866"
     CDS             30203..31672
                     /gene="rhaB"
                     /locus_tag="ACHVB7_000866"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR1912932.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31669..32928
                     /gene="rhaA"
                     /locus_tag="ACHVB7_000867"
     CDS             31669..32928
                     /gene="rhaA"
                     /locus_tag="ACHVB7_000867"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR1912933.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33170..33994
                     /gene="rhaD"
                     /locus_tag="ACHVB7_000868"
     CDS             33170..33994
                     /gene="rhaD"
                     /locus_tag="ACHVB7_000868"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR1912934.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34004..34318
                     /gene="rhaM"
                     /locus_tag="ACHVB7_000869"
     CDS             34004..34318
                     /gene="rhaM"
                     /locus_tag="ACHVB7_000869"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR1912935.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34619..35065
                     /locus_tag="ACHVB7_000870"
     CDS             34619..35065
                     /locus_tag="ACHVB7_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR1912936.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            35076..36605
                     /locus_tag="ACHVB7_000871"
     CDS             35076..36605
                     /locus_tag="ACHVB7_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFR1912937.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVFLVCYLANDKALLGTQSGAGFLGAVVLGLAIGYFVFWFRKV
                     RLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAA
                     FLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPK
                     LFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAFGIKR
                     LAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHE
                     H"
     gene            36595..37665
                     /locus_tag="ACHVB7_000872"
     CDS             36595..37665
                     /locus_tag="ACHVB7_000872"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1912938.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37665..39413
                     /locus_tag="ACHVB7_000873"
     CDS             37665..39413
                     /locus_tag="ACHVB7_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR1912939.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39462..>39748)
                     /locus_tag="ACHVB7_000874"
     CDS             complement(39462..>39748)
                     /locus_tag="ACHVB7_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HFR1912940.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9640 a        10326 c        10347 g         9435 t
ORIGIN      
        1 ccaccggcgc gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca
       61 gcggggcggg cagggcgatg tcggtttcac cgggaaggac cttcgcaccg agcagcggat
      121 tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg ccggtggggg
      181 caccgatgcg caccccggct gaaccctgga caatgctacc gccatactgc gtcatgtcgc
      241 cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttgaaataaa taagaaccag
      301 aagcctagcg ccagtcaaca acaggaaatg ataatgagtt aacaccccgg aatttttcag
      361 aatttataaa aggcaaatta caggcacaaa aaaagcgccg tgcggcgctt ttttcggaaa
      421 tccggtctta tttgctgccc gggatgttga aacgcttgtt gaagcggtca acacggccac
      481 cggtagcaac atcacgctgt ttgccagtga agaacgggtg gcacttgctg cacacgtcga
      541 ggttcaggtc atgaccaacg gtggagcgga ttttcattac gttaccgcaa gagcagctag
      601 cagtaatttc ttcgtatttc gggtgaatat cttttttcat gggaaaacct cggtttaagg
      661 ccgcgtcgct cttccagccc taacgccaga caccacgcga tgttaaaagt atagcttcaa
      721 tacgatcatt tcgtacgaag gcgcgaaatc atacagaaat taaccagcgt atgcaaactg
      781 atccgcactc ttctacggca atgtgtatac taacccaccg aatttcaagt caggatgatg
      841 ctatgcccgt tgcccacgtt gccttgcccg ttccgcttcc tcgtaccttt gactatctgc
      901 tgccagaagg catggcggtt aaagccgggt gtcgcgtgcg cgtgccgttt ggcaaacagc
      961 aggagcgcat cggggttgtg gtatcagtta gcgatgtcag tgagctgccg cttaacgagc
     1021 tgaaagcggt agttgaagtg ctggacgttg agccggtatt tactcactcc gtctggcgat
     1081 tactgctctg ggcggcagat tactatcatc atccgattgg cgatgtgctg tttcatgcct
     1141 tgccgatttt actgcgccag gggcggcctg cggcgaacgc gccgatgtgg tactggtttg
     1201 ccactgaaca aggccaggcg gtggatctga acagcctgaa acgctcaccc aagcaacaac
     1261 aggcgctggc ggcgttacgg caagggaaaa tctggcgcga ccaggttgcc acgctcgaat
     1321 ttaatgatgc cgcgttgcag acgctgcgca aaaaaggtct gtgtgattta gcaagtgaaa
     1381 caccagagtt tagcgactgg cgaacgaact atgccgtttc tggtgagcgg ttgcgattga
     1441 ataccgaaca ggccaccgcc gttggcgcaa ttcatagcgc ggcagatact ttttctgcct
     1501 ggctgctggc gggcgttacc ggttccggta aaacggaggt ttatctcagt gtactggaaa
     1561 acgtgctcgc tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc ctgacaccgc
     1621 aaactatcgc ccgttttcgt gaacggttta atgcgcccgt ggaagttctg cattccggtc
     1681 tgaacgacag cgagcgtctt tcggcgtggc tgaaagcgaa aaatggcgag gcggcgattg
     1741 tgatcggcac ccgctccgcg ctgtttacgc cgtttaaaaa tctcggcgtg attgtcattg
     1801 acgaagagca cgacagttcc tacaagcagc aggaaggctg gcgctatcat gcccgcgacc
     1861 tggcggtgta tcgtgcgcat agcgagcaaa tcccgattat tcttggctcc gcaacgcccg
     1921 cgctggaaac gttgtgcaac gtccagcaga aaaaataccg cctgctgcgc ctgacgcgcc
     1981 gggcggggaa tgcgcgtccg gcaattcaac acgtgctgga tttaaaaggt cagaaggtgc
     2041 aggcaggtct ggctccggcg ttaatcaccc gtatgcgcca gcatttacag gccaacaatc
     2101 aggttatcct ctttcttaac cgccgtggct ttgcgcctgc gctactgtgc cacgactgcg
     2161 gctggattgc cgaatgccca cgttgcgatc actactacac gctgcatcag gcgcaacagc
     2221 atttgcgctg tcaccactgt gacagtcagc gtccggtgcc gcgccagtgc ccttcctgcg
     2281 gttccacgca cctggtaccc gtggggctgg gcaccgaaca gcttgaacag acgcttgcgc
     2341 cgctgttccc tgatgtgccc atttctcgta tcgaccgcga taccaccagc cgcaaagggg
     2401 cgctggaaca gcaactggcg gaagtacatc gcggcggtgc gaggattttg atcggcacac
     2461 aaatgctggc gaaaggtcac cactttccgg atgtgacgct ggtggcgtta ctggatgtgg
     2521 acggcgcgct gttttctgcc gattttcgct cggcagagcg tttcgctcag ctttacaccc
     2581 aggtcgccgg tcgtgccggg cgtgcgggta aacagggcga agtggtgctg caaacgcacc
     2641 atccggaaca tcctctgttg caaacgttgc tctataaagg ctacgacgcc tttgccgaac
     2701 aggcgctggc tgagcgacgc atgatgcagc tgccgccgtg gaccagccat gtgattgtgc
     2761 gtgcggaaga tcataacaat cagcatgcgc cgctgttcct gcaacaactg cgtaatctga
     2821 tcctctccag cccgctggca gacgacaaat tgtgggttct cggtccggtt ccggcactgg
     2881 cacctaaacg tggcggtcgc tggcgctggc agatattgtt gcagcaccct tcccgcgtgc
     2941 gcttgcaaca catcattaac ggtacgctgg cactcatcaa tacaataccg gattcccgta
     3001 aggtgaaatg ggtgctggat gttgatccga ttgagggtta aaccgctcac gatgcgaggc
     3061 ggatcgaaaa attcaatatt catcacactt ttcatgaaaa ttctgtaacc gttttcacgc
     3121 gctatctgct aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg cctgacgtgc
     3181 caggcgagga gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca tgaaagacgt
     3241 tgccctcaag gcaaaagtct ctacagcgac tgtctcccga gcattaatga atcccgataa
     3301 agtctcccag gccacccgta atcgggttga aaaagcggcc cgggaagtgg gttatttacc
     3361 gcagcctatg gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg tgattgtccc
     3421 ggatatctgc gatcccttct ttagcgaaat tattcgcggt atcgaagtta cggcggcaaa
     3481 tcacggatat ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg aaaaaacctt
     3541 tatcgatttg atcatcacca agcaaattga tggcatgttg ctgctgggtt caaggctgcc
     3601 gtttgatgcc agcattgagg aacagcgtaa tctgccgccg atggtgatgg cgaacgaatt
     3661 cgcaccggaa ctggagctac ctacagttca tatcgacaat ctgaccgccg catttgatgc
     3721 agtaaattat ctatatgagc aagggcataa acggattggc tgtatagccg gtcccgaaga
     3781 gatgccgctg tgtcactacc gcctgcaagg ttacgttcag gcgctgcgtc gctgcggcat
     3841 tatggttgat ccgcaataca tcgcccgtgg cgacttcacc ttcgaagccg gaagcaaagc
     3901 gatgcagcag ctgcttgatc ttccacaacc gcctactgct gtcttctgcc atagcgatgt
     3961 gatggcgctc ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc cggaagacct
     4021 ttccataatc ggttttgata acatcgacct gacgcaattt tgtgatccgc cgctgacaac
     4081 catcgcgcag ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc ttgatcaaat
     4141 gcaggggcaa cacgttggca gtggctctcg tttaatggac tgcgaactta tcatccgggg
     4201 atcaacacgc gcgttacctt aaagtaaacc tttaagactg ccgtgactgg tcaaaggccc
     4261 gccgcttaag taacatggcg ggctgacgaa cgaataaata cagcgaaacg atagtggcac
     4321 aacgagatta tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag agcacctcac
     4381 ggaaaaagca acgaaatctg cctgcggttt ctcccgctat ggtcgctatt gctgccgccg
     4441 tgcttgtgac ctttatcggt ggtctgtact tcattacgca tcacaagaaa gaagagtccg
     4501 agacgctgca aagccagaaa gtgaccggta acggactacc accaaaacca gaagaacgct
     4561 ggcgctacat taaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc acagaacctt
     4621 ctgccggtgg tgaagtgaaa acgccggagc aactgacacc agaacaacgt cagcttcttg
     4681 aacaaatgca ggctgatatg cgccagcagc caacgcagct ggttgaagtt ccgtggaatg
     4741 aacagacgcc ggaacagcgt cagcaaacgc tacaacgcca gcgtcaggcg cagcaattag
     4801 cggagcagca acgcctggct cagcagtcgc gtacgactga acaaagctgg cagcagcaga
     4861 cgcgtacgtc gcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa ccggcttcta
     4921 ctcagcagcc gtaccaggat ctgctgcaaa cccctgcgca cacgaccgcg cagtcgaagc
     4981 cgcagcaagc tgcgccggtt gctcgtgccg ctgacgcgcc aaaaccgacg gcggagaaaa
     5041 aagacgaacg ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa caggcagaga
     5101 cggtacgtgc tcagctggcg ttcgaaggat ttgactcgaa aatcaccacc aacaatggct
     5161 ggaatcgtgt ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc accctcaatc
     5221 ggttgaagat ggcgggtcat acaaactgca ttcggctcgc cgccgggggt tgaaaccctc
     5281 aaaatccccc ccatctataa ttgcattatg ccccgtactt ttgtacgggg tttgtactct
     5341 gtattcgtaa ccaaggggtc agctcgtgac aactatagta agcgtacgcc gtaacggcca
     5401 tgtggtcatc gctggtgatg gtcaggccac gttgggcaat accgtaatga aaggcaacgt
     5461 gaaaaaggtc cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg gcggtactgc
     5521 ggatgctttt acgctgttcg aactgtttga acgtaaactg gaaatgcatc agggccatct
     5581 ggtcaaagcc gccgttgagc tggcaaaaga ctggcgtacc gatcgcatgc tgcgcaaact
     5641 tgaagcactg ctggcagtcg cggatgaaac tgcatcgctt atcatcaccg gtaacggtga
     5701 cgtggtgcag ccagaaaacg atcttattgc tatcggctcc ggcggccctt acgcccaggc
     5761 tgcggcgcgc gcgctgttag aaaacactga acttagcgcc cgtgaaattg ctgaaaaggc
     5821 gttggatatt gcaggcgaca tttgcatcta taccaaccat ttccacacca tcgaagaatt
     5881 aagctacaaa gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa tcgtcagcga
     5941 actggataag cacatcatcg gccaggacaa cgccaagcgt tctgtggcga ttgctctgcg
     6001 taaccgctgg cgtcgcatgc agctcaacga agagctgcgc catgaagtga ccccgaaaaa
     6061 tatcctgatg atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc gtctggctaa
     6121 gctggcgaat gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag tgggctatgt
     6181 cggtaaggaa gtggattcta ttattcgcga tctgaccgat gccgccgtga aaatggtacg
     6241 cgtccaggct atcgagaaaa accgttatcg cgctgaagaa ctggcagaag aacgtattct
     6301 cgacgtgctg atcccacctg ctaaaaacaa ctggggacag accgaacagc agcaggaacc
     6361 gtccgctgct cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg atgacaaaga
     6421 aatcgagatc gatcttgccg cagcaccgat gggcgttgaa attatggctc ctccaggcat
     6481 ggaagagatg accagccagc tgcagtccat gttccagaac ctgggcggcc agaagcaaaa
     6541 agcgcgtaag ctgaaaatca aagacgccat gaagctgctg attgaagaag aagcggcgaa
     6601 actggtgaac ccggaagagc tgaagcaaga cgctatcgac gctgttgagc agcacgggat
     6661 cgtgtttatc gacgaaatcg acaaaatctg taagcgcggc gagtcttccg gtccggatgt
     6721 ttctcgtgaa ggcgttcagc gtgacctgct gccgctggta gaaggttgca ccgtttccac
     6781 caaacacggg atggtcaaaa ctgaccacat tctgtttatc gcttctggcg cgttccagat
     6841 tgcgaaaccg tctgacctga tcccggaact gcaaggtcgg ctgccaatcc gcgttgaact
     6901 gcaggcactg accaccagcg acttcgagcg tattctgacc gagccgaatg cctctatcac
     6961 cgtgcagtac aaagcactga tggcgactga aggcgtaaat atcgagttta ccgactccgg
     7021 tattaagcgc atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa acatcggtgc
     7081 tcgtcgttta cacactgttc tggagcgttt aatggaagag atttcctacg acgccagcga
     7141 tttaagcggt caaaatatca ctattgacgc agattatgtg agcaaacatc tggatgcgtt
     7201 ggtggcagat gaagatctga gccgttttat cctataatcg cgttcaatca ttttcatcat
     7261 tgtttgatgg ggctgaaagg ccccattttt attggcgcgt attatgactg aacaacaaat
     7321 tagccgaact caggcgtggc tggaaagttt acgacctaaa accctccccc tcgcctttgc
     7381 tgcaattatc gtcgggacgg cgctggcatg gtggcaaggt cacttcgatc cgctggtcgc
     7441 cctgctggta ctaattaccg ccgggctatt acagatcctt tctaacctcg ccaatgatta
     7501 cggcgatgcg gtaaaaggca gcgataaacc tgaccgcatt gggccgctac gcggcatgca
     7561 aaaaggggtc attacccagc aagagatgaa acgggcgctc attattaccg tcgtgctcat
     7621 ctgtctctcc gggctggcac tggttgcagt ggcatgccat acgctggccg attttgtcgg
     7681 tttcctgatt cttggcgggt tgtcgatcat tgccgctatc acctacaccg tgggcaatcg
     7741 tccttatggt tatatcggtc tgggtgatat ttccgtactg gttttctttg gctggttgag
     7801 cgtcatgggg agctggtatt tacaggctca tacattgatt ccggcactga tccttccggc
     7861 gaccgcgtgt ggcctgctgg caacggcagt actgaacatt aataacctgc gtgatatcaa
     7921 tagcgaccgc gaaaatggca aaaacacgct ggtggtgcgc ttaggtgaag tgaacgcgcg
     7981 tcgttatcat gcctgcctgc tgatgggctc tctggtatgt ctggcgctgt ttaatctctt
     8041 ttcgctgcat agcctgtggg gctggctgtt cctgctggcg gcaccattac tggtgaagca
     8101 agcccgttat gtgatgcggg aaatggaccc ggtggcgatg cgaccaatgc tggaacgtac
     8161 tgtcaaggga gcgttactga ctaacctgct gtttgtttta gggatattcc taagccagtg
     8221 ggcagcataa ctgacaaata tcaattaaca attgatgatt ttgccaacag cctacatagc
     8281 gcgatatact gaaaattctc gcagcaactg aatgttaagc ctatgaaata cgatacttcc
     8341 gagctttgtg acatctatca agaagatgtt aacgtcgtgg aaccgctgtt ctccaacttt
     8401 ggcggacggg cgtcgtttgg cggacaaata atcacggtaa aatgtttcga ggacaacggg
     8461 ttgctgtacg atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt cgatggcggt
     8521 ggttctgttc gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt acaaaatgaa
     8581 tgggaaggtc tggttattta cggcgcggtg cgtcaggtag atgacctaga agagttggat
     8641 atcggcatcc aggcgatggc ggcaattccg gttggtgccg ctggcgaagg cattggcgaa
     8701 agcgatgtcc gcgtcaattt tggcggtgta accttcttct ccggcgacca tctttatgcc
     8761 gacaataccg ggattattct ttcagaagat ccgctggata ttgaatgata agaaaggcac
     8821 cgcaaggtgc cttttttctg cgttacctgt tggcctacac agtaaagaaa ttacgcgaaa
     8881 gatgaagcgt aatcagacct cttccatgcg acccagcagg gcctgcagac gttcctgcca
     8941 gccgttctgc tgttctttca gatggttgtt ctcacgctcc agctcttcgc gctgatgctg
     9001 ggcattttga acttcctgcg acagtgagtt gtttttttct ttcagctctt cgatttccat
     9061 ctgcaacaga gtgatggtat caatcgcctg ctgtactttt gcttccagtt tctcaaacac
     9121 ttctaatgac attgtcatac ctctcctgaa ttgcaaggcg ttgatggata aaaaatcctc
     9181 gtcccgatta ccggtgacgc cttaataaat acgagcgcac tttagttagc tccgattgta
     9241 tgaagccgcg ccatcgctgt ccagcggcac gccttgcaga ttacggtttg ccacactttt
     9301 catccttctc ctggtgacat aatccacatc aatcgaaaat gttaataaat ttgttgcgcg
     9361 aatgatctaa caaacatgca tcatgtacaa tcagatggaa taaattgcgc gataacgctc
     9421 attttatgac gaagcacaca cattttaagt tcgatatttc tcgtttttgc tcgttaacga
     9481 taagtttaca gcatgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa
     9541 cattaactct tcaggatccg attatgagtc aaacatcaac cttgaaaggc cagtgcattg
     9601 ctgaattcct cggtaccggg ttgttgattt ttttcggtgt gggttgcgtt gcagcactaa
     9661 aagtcgctgg tgcgtctttt ggtcagtggg aaatcagtgt catttgggga ctgggggtgg
     9721 caatggccat ctacctgacc gcaggggttt ccggcgcgca tcttaatccc gctgttacca
     9781 ttgcattgtg gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt atcgtttcac
     9841 aagttgccgg cgctttctgt gctgcggctt tagtttacgg gctttactac aatttatttt
     9901 tcgacttcga gcagactcat cacattgttc gcggcagtgt tgaaagtgtt gatctggcag
     9961 gcactttctc tacttaccct aatcctcata tcaattttgt gcaggctttc gcagttgaga
    10021 tggtgattac cgctattctg atggggctga tcctggcgtt aacggacgat ggcaacggtg
    10081 taccacgcgg ccctttggct cccttgctga ttggtctact gattgcggtc attggcgcat
    10141 ctatgggccc attgacgggt tttgccatga acccagcgcg tgacttcggt ccgaaagtct
    10201 ttgcctggct ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac attccttact
    10261 tcctggtgcc gctttttggc cctatcgttg gcgcgattgt aggtgcattt gcctaccgca
    10321 aactgattgg tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag gagaccacaa
    10381 ctccttcaga acaaaaagct tcgctgtaat atgactacgg gacaattaaa catgactgaa
    10441 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    10501 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    10561 ggctgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    10621 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    10681 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    10741 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    10801 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    10861 tggatcctcg accatgtgga aggctctcgc gagcgtgcgc gtcgtggtga attgctgttt
    10921 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    10981 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    11041 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    11101 gtgtacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    11161 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    11221 aacacctatg gcactggctg ctttatgctg atgaacaccg gcgagaaagc ggtgaaatca
    11281 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgctg
    11341 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    11401 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    11461 tatgtggttc cggcctttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    11521 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    11581 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    11641 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    11701 tccgatattc tcggcacccg cgttgagcgc ccggaagttc gcgaagtgac tgcattgggc
    11761 gcggcctatc ttgctggtct ggcggtcggt ttctggcaga acctcgacga gctgcaagag
    11821 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    11881 tacgcaggct ggaaaaaagc ggttaaacgc gcgatggcgt gggaagaaca cgacgtaatg
    11941 taaatgccgg atgaagcgtt tatgccgcat ccggtagtcc cgaaacgtgc gggggcaacc
    12001 ccgcacacat caataatccc tcccttcccc tgtgctacac ttcgcgccat tccttactgc
    12061 ttagagtttg ctatgagacg agaacttgcc atcgaatttt cccgcgtcac cgaatctgcg
    12121 gcgctggctg gctacaaatg gttaggacgc ggcgataaaa acaccgcgga cggcgcggcg
    12181 gtaaacgcca tgcgtattat gctcaaccag gtcaatattg acggcaccat cgtcattggt
    12241 gaaggtgaaa tcgacgaagc accgatgctc tacattggtg aaaaagtcgg tactggtcgc
    12301 ggcgacgcgg tagatattgc tgttgatccg attgaaggca cgcgcatgac ggcgatgggc
    12361 caggctaacg cgctggcggt gctggcagta ggcgataaag gctgcttcct caatgcgccg
    12421 gatatgtata tggagaagct gatcgtcgga ccgggagcca aaggcaccat tgatttgaac
    12481 ctgccgctgg cggataacct gcgcaatgtt gcggcggcgc tcggcaaacc gttgagcgaa
    12541 ctgacggtaa caattctggc taaaccacgc cacgatgccg ttatcgctga aatgcaacaa
    12601 ctcggcgtac gcgtatttgc tattccggat ggcgacgttg cggcctcaat tctcacctgt
    12661 atgccagaca gcgaagttga cgtgctgtac ggtattggtg gcgcgccgga aggcgtagtt
    12721 tctgcggcgg tgatccgcgc attagatggc gacatgaacg gtcgtctgct ggcgcgtcat
    12781 gacgtcaaag gcgacaacga agagaatcgt cgcattggcg agcaggagct ggcacgctgc
    12841 aaagcgatgg gcatcgaagc cggtaaagta ttgcgcctgg gcgatatggc gcgcagcgat
    12901 aacgtcatct tctctgccac cggtattacc aaaggcgatc tgctggaagg cattagccgc
    12961 aaaggcaata tcgcgactac cgaaacgctg ctgatccgcg gcaagtcacg caccattcgc
    13021 cgcattcagt ctatccacta tctggatcgc aaggacccgg aaatgcaggt gcacatcctc
    13081 tgattgattt gatcgattga gccttccagt ccttcgggac tggaattttt ttgttcggag
    13141 aacgaagata aggcaagtca atcaaaacag gagaaaaaca tggctgattg ggtaacaggc
    13201 aaagtcacta aagtgcagaa ctggaccgac gccctgttta gtctcaccgt tcacgccccc
    13261 gtgcttccgt ttaccgccgg gcaattcacc aagcttggcc ttgaaatcga cggcgaacgc
    13321 gtccagcgcg cctactccta tgttaactcg cccgataatc ccgatctgga gttttacctg
    13381 gtcaccgtcc ccgatggcaa attaagccca cgactggcgg cactgaaacc aggcgatgaa
    13441 gtgcaggtgg ttagcgaagc agctggcttc tttgttctgg aggaagtacc agattgcgaa
    13501 acgctatgga tgctggcaac cggtacagcg attggccctt atttatcgat tctgcaacta
    13561 ggcaaagatt tagatcgctt caaaaatctg gtcctggttc acgccgcacg ttatgccgcc
    13621 gacttaagct atttgccact gatgcaagaa ctggaaaaac gctacgaagg caaactgcgc
    13681 attcagacgg tggtcagtcg ggaaacggca gcggggtcgc tcaccggacg gataccggca
    13741 ttaattgaaa gtggggaact ggaaagcgcg attggcctgc cgatgaataa agaaaccagc
    13801 catgtgatgc tgtgcggcaa tccacagatg gtgcgcgata cacaacagtt gctgaaagag
    13861 acccggcaga tgacgaaaca tttacgtcgc cgaccgggcc atatgacagc ggagcattac
    13921 tggtaagcgg ttacttatcg ataaacggca cgatgagcaa atccgcactc atcttattga
    13981 tcatcccgcg atatgccggc atcaaacggt tgataaatga gtggtgatga ccacagacaa
    14041 ggaggtcgca ctgctctttt tgcataattt ccagcagtgt ttccggcatt tctccgcgtt
    14101 caatacgcag ttttgtcttc ggccattgaa tatttttcgt cagtttatac agcttgttat
    14161 ccgacttatt cttcaacaat tgaagaatat cttctgttgc agggaagtag atacccggat
    14221 acaactcgct taagccatca tcaatatgaa ttaacgtcag gtgagcgtca ttatgtctgg
    14281 cgagctccag ggctttattc accagtaagg catcttcttc attcccggaa attgccacgc
    14341 caatgtgttt ataagccatg tttaactcct tctaaagcaa ctccatcaag ctaacgaacg
    14401 cagtgataac tcaaaaataa tcatctcggc ctggcatgag aaagtgaagg ccgcatcaag
    14461 ctcaacttgc ccctccagat ccttaatctt ataggccacg ctggcccctt catctgcaga
    14521 ggatgcagcg gcggcaagct ctttcagttt ggtcataaaa gactctgctt cttcgcgggt
    14581 ggcaaaaaag cgcgaaaact tactggtaca gttgtcgtta tcaatcaccg taccgatatc
    14641 aatcgcacat cctttagtac tgcatttatc tacgacatct ttcatagggc acctctgtat
    14701 attcgcctct ctgtcgatta cgacttaaga ataatgcgcc gttaagggaa gaacaaacaa
    14761 gtaatgatct tatggattac catttttttt gatattgctt aaggtgaaga gttcagaagg
    14821 attttccggg tattgtcatt tgcatcgtgg aaataacaat cccggaaaaa aatagcagta
    14881 agagatatat taaaggttaa ttacttgatt tattgtcggc tttatacttc acatcctgag
    14941 tatctttacc atatttattt tcgccttgtg tgccaacaaa cgcaccaaga tcgataagca
    15001 tcatcaccag aatcaacgtc gggacaaaac gccccaccgc ccattgccag acacccggta
    15061 aaatcgccca gttacccgcc agcagcatcc acgccacaat catcagaaat gcccatgcgc
    15121 cggaacgccc gcgatcatgc aagcgcttaa cagttactgc cgctgttggc caaagcaagc
    15181 acacaaggca aaacgccgcg gtctgaatat cgagtaaatt tttacccgcc agtgagaaaa
    15241 gcaccagcat gcctgcgaac cacaggccta tccaaatcca gaaatcacgg cgtccaatac
    15301 gccctttaaa tgagaataac cattgctgta tggtcatgta agttccttga tggttgtctt
    15361 ttccaggatt ctaccctttt gacaagggcg acagatatcg ttttaatcgg agccagtcat
    15421 aacaaaaggt actgtcaatg aagccagggt gtacgctgtt ttttctctta tgttctgcat
    15481 taaccgttac aacaacggcg catgcacaaa caccagatac ggcaacgacc gcgccttatc
    15541 tgctggctgg agcccctact ttcgatctct ccatcagcca gtttcgagaa gactttaaca
    15601 gccagaatcc tagcctgcca ctgaacgaat ttcgtgccat cgacagcagt cccgacaaag
    15661 ccaatctcac tcgtgctgca agtaaaatta acgagaactt gtatgcttct acagcgctgg
    15721 agcgcggtac cttaaaaatc aaaagcattc aaatgacctg gctacccatc caggggccag
    15781 agcaaaaagc agcgaaagcg aaagctcagg aatacatggc agcagtgatc cgcacactca
    15841 ccccattaat gaccaaaaca caaagccaga aaaaactgca gtcgctacta acggcgggga
    15901 aaaacaaacg ttattacacc gagacagaag gtgcactgcg ttatgttgtc gcggacaaca
    15961 gcgaaaaggg gctgaccttc gctgttgaac cgattaagct ggcgctatct gaatcgcttg
    16021 aaggtttgaa taaatgacaa aaagcaaagc ctttgtgccg atgaatctct atactgtttc
    16081 acagacctgc tgccctgcgg ggcggccatc ttcctttatt cgcttataag cgtggagaat
    16141 taaaatgcga catcctttag tgatgggtaa ctggaaactg aacggcagcc gccacatggt
    16201 tcacgagctg gtttctaacc tgcgtaaaga gctggcaggt gttgctggct gtgcggttgc
    16261 aatcgcacca ccggaaatgt acatcgatat ggcgaagcgc gaagctgaag gcagccacat
    16321 catgctgggt gcgcaaaacg tggacctgaa cctgtccggc gcattcaccg gtgaaacttc
    16381 tgccgctatg ctgaaagaca tcggcgctca gtacatcatc atcggccact ctgaacgtcg
    16441 tacttaccac aaagagtctg acgaactgat cgcgaaaaaa ttcgcggtgc tgaaagagca
    16501 gggcctgact ccggttctgt gcatcggtga aaccgaagct gaaaacgaag cgggcaaaac
    16561 tgaagaagtt tgcgcacgtc agatcgacgc ggtactgaaa actcagggtg ctgcggcatt
    16621 cgaaggtgcg gttatcgctt acgaacctgt atgggcaatc ggtactggca aatctgcaac
    16681 tccggctcag gcacaggctg ttcacaaatt catccgtgac cacatcgcta aagttgacgc
    16741 taacatcgct gaacaagtga tcattcagta cggcggctct gtaaacgcgt ctaacgctgc
    16801 agaactgttt gctcagccag atatcgacgg cgcgctggtt ggcggtgctt ctctgaaagc
    16861 tgacgctttc gcagtgatcg ttaaagctgc agaagcggct aaacaggctt aagtctgaca
    16921 ggtgccggat ttcatatccg gcacttactt tccttaactc ttcgccttaa cgcaaaatct
    16981 cacactgatg atcctgaatt tcctcggctg aagcacggtt aagcgtcagt agatttcgtt
    17041 gtgtcgccag caatacaaat gagttatcac tctgccgtac catcgccagc ccgtagcttc
    17101 ccatatgttc ccgcgcctca ggtacttctt ctgccagcat cataaatggg ctgcgttgta
    17161 ccagttcgct ttccgttacc cgacgcgcca ggtattcatg cccacgcaaa ccacctggca
    17221 gtggcaacca gcggctgctg atgttcgcca gattgttatc cagttgttcg cgcacatcag
    17281 gacgaataca agagatatga atatgaaaat ggttttgcgt acgcccggtg cgggagttga
    17341 tcgccaaaga aaccgcgcga tcgggaaccg gctggccgta ttttttgctc ataaaatcac
    17401 gcgcctgcca ggccaaccaa aagaagttcg gcgttgaagg atcggtcaac aaaggacttt
    17461 cagtaccgtt aatacgatac gttggcatca acagatattg cagtggacca ttaagatctt
    17521 ttaaaaccac gtatccggca ttgggtttga cttccgcaca tggcgaagga ttttgatttt
    17581 gctgctgatt gggcaaacat tcctcaagga caatcttacg taatgtatcc gactcttcac
    17641 cggttaattt ccagtaacca ataccggcag ccacaacggc gataactatc atcaccaaaa
    17701 aaagaagacc cgcttttttc atcttttttt ccctgtacct caaagagatg caagggtaac
    17761 gcaaaatcgt gacaaataaa aaacccggtc gcgagaaacc accgggtttg ataattatcc
    17821 tggggaaatc agcgtttgct gatctgatcg aacgtaccgc cgttagcaaa atgctctttt
    17881 tgcgctttcg tccagccgcc gaactcttca tcaatggtga ataacttcag ctttggaaac
    17941 gcattttcgt actttttcgc tacttcagcg tcgcgcggac ggtagtagtt tttcgcggca
    18001 atttcctgac cttctggcga gtagagatat ttcaggtagg cttccgccac ctctttggtg
    18061 ccttttttct cgaccacttt atcgaccacc gacacggttg gctctgcgag gatagactca
    18121 ctcggcgtga cgatttcgaa tttatctttc cccagttcat tcgctgccag cagagcttcg
    18181 ttttcccagg cgatcagtac atcgccaatc ccgcgctcga caaaggtgtt agtggagccg
    18241 cgcgcgccag aatccagaac ttcgacgttt ttatacagtg cccgaacaaa atcctgtgct
    18301 tttgcctgat cgttgttgtt gtgatgcagc gcgtagcccc aggcagctag gtagttccag
    18361 cgtgcgccac cagagctttt cggattaggc gtgatcaccg aaacacccgg tttaatcaga
    18421 tcgttccagt catggatctg cttcggattg cccttacgca ccaggaaaac aatggtggaa
    18481 gtgtacggtg cggagttatc cggcagacgt ttgatccact ctttatcaat ccgcccacgt
    18541 tccgcaatcg cgtccacgtc ataggccaga gccagcgtga caacatcagc ttcaataccg
    18601 ttgattaccg acgtcgcttg tttacctgaa ccaccgtgtg actgacgaat caccacgtta
    18661 tcaccagttt gctgtttcca gtgggcgctg aatgccttgt tgtactgttc gtacaattcg
    18721 cgcgttggat catatgaaac gttaagaagc tgaatatcct ttgccataac gctggttgcc
    18781 gccagcaaaa atgttaaccc tacgccccac ttgttcatcg cccgactctc ttatgttgtg
    18841 ttgtgatgag caaagcgtgc cagaaggtta accaaacatt aaagaataaa aaaagattgg
    18901 ctataacttg cgggtatatg ttgagggatt aaaaaggcgg aagaaatccg cctcatattg
    18961 ctgacaaagt gcgctttgtc catgccggat gcggcgcgaa cgccttatcc ggcctacaaa
    19021 agtttgcaaa ttcaataaat tgcagaattc atgtaggcct gataagcgaa gcgcatcagg
    19081 catttttgct tctgtcatcg gtttcaggct aaaggaatct gcctttttcc gaaatcatta
    19141 atacagtttt ttcgcgcagt ccagccagtc acctttgaac ggacgcttca tgttttcgat
    19201 agcgtcgatg atgtcgtggt gaaccagctg ttcgttctgg atacctacac aacgaccgcc
    19261 gtaacctgcc agcagcagat cgatagcgta agcgcccata cgggaagcca gaatacggtc
    19321 gtaaggcacc ggagaaccac cgcgctggat gtggcccagc acagttgcgc gggtttcacg
    19381 accggtttct ttctcgatga aatgcgccag ttcgtcaaca tcacacatat gttcggtaat
    19441 cgccacgatc gcgtgttttt tacctttcgc gatacccgct ttgatttcgt ttaccaggtc
    19501 ttcacggctg aattcaactt ccggaaccac aacgaattca cagccaccgg caatggccgc
    19561 agccaacgtc agatcgccac aataacggcc catcacttcc accacggaaa tacgctggtg
    19621 agaagaagag gtgtcacgca gacggtcgat cgcttctaca acggtgctca gcgcagtgaa
    19681 gaaaccgata gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg gcagaccgat
    19741 gcacgggaag cccatttcgg tcagacgcat tgcacccatg taggaaccgt caccgccgat
    19801 aaccaccagc gcgtcgatac cacgtttttt caggttttcg atagccacgg cgcggatgtt
    19861 ctcgtcgcgg aattccggga agcgcgcaga accgaggaac gtaccgccac ggttgatcat
    19921 gtcagaaacg ctgtaacggt ctagctgtac catacggtct tcatacagac ccagatagcc
    19981 gtcataaatg cccattactt ccagaccttc tgtcagcgca gaacgaacaa ccccgcgaat
    20041 tgcggcgttc atgcctggcg catcaccgcc gcttgtcaac acaccgattt tcttaatcat
    20101 gactacctct gaactttgga atgcaaaata aaatctgttg ccggaagtct tcttgcacat
    20161 cgaagtgatc caacgaatgt gcaaatagta taacaatcac ttcctgctga attgattcag
    20221 gtcaggccaa atggcggtat tttatacaca aaatgcgggt ctggctctct tttatactga
    20281 ttatgaaagc atagaccgtt taccctccct gggtacgacg gaacaggggt cctgatggat
    20341 aattacatcc gatcccggaa aacgccgtaa aatagcctgc tctacctgat ccgccaccat
    20401 atgtgcctga accaaaggca gagagtcttc catttccaaa tgaatctgaa taaagcgggt
    20461 cggccctgac tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc aggaagtcac
    20521 gatatcaata atttcttgcc gttcctcatc aggcaatgcg cgatccagta atgactgtac
    20581 cgcctcatat cccatgcgta acgcgctata taaaatatag atgccgattc ccaatgcaaa
    20641 cagagcatcg gcgcgatgcc agccgtacca ggacaacccc agcgccagca gaattgcgcc
    20701 gttcatcata acatcagact ggtaatgtag catatcagcc cgcaccgcct ggctttgcgt
    20761 ccggcgcacc acccaccgct gaaacgagac aaggataatc gtacaaatta gcgccacaat
    20821 tgtcacgata accccgacgc ctggatctgt catcggtgtt ggagatacca gatgttgaat
    20881 acccgtcaaa aacaggaata gtgccgaacc ggagataaac atactttgcg ccagcgccgc
    20941 gagggactca gctttaccgt gaccaaacga gtgattatcg tcggcaggtt gcagggaata
    21001 tcgcaccacc agtaaattcg tcaacgacgc gccgatatcc accagcgaat ccaccagcgc
    21061 ggcgagaata ctcaccgacc cggtatacca ccatgcaaaa attttaatca gcaatagcag
    21121 cgaagccatc gccgtcgcag caatcgccgc ccgactgacc agccgtccat aagattgatt
    21181 cataaatact cccactatca actgacgcta gtataacgga agcaaatcat ctgcaatgca
    21241 ttaagcagca ggcaaattga ggataaaaaa aaccccacat catgtggggg aagacaggga
    21301 tggtgtctat ggcaaggaaa acagggttta ctactgggaa cgtgagttgc tactactcaa
    21361 tagcttcaac gatgaacttt tttgccattg cgtcacgtca cgcaactgct ccattcgttg
    21421 ttgatgtttc tcgtttaaaa ccgcttgctg ctccggcgtt aacaggcgat acatttggtt
    21481 gcggactttt gccatctcaa cctgacgagc aatttgctca ttcgccattt tttctgcctg
    21541 tgcgcgcaca gcgttttcat caaaattttc tgcggtgaca aggcgatgca ttgtctccag
    21601 ttcgctaaca ttaacaggag gctgttcgtg ccgggcctgt tgcataagat ctcgcatctg
    21661 ctgacgctga tgttcggtta aacttatgcc gtcgaacata tggctctgcg tactgcgctg
    21721 cgtaagttct tcacccggat gccagttatc gcctgaaccg acttcagcag cgtggcttaa
    21781 tgaactgact gccagcgttg aggccatgac ggcagcggta actatgcgca tcatttgctc
    21841 ccaaaatctt tctgtcgcga ttcaacgata gagagtttac gattcaggct gcaaacatgc
    21901 gtcagggggt gtaaaacaac gtaaagtcat ggattagcga cgtctgatga cgtaatttct
    21961 gcctcggagg tatttaaaca atgaataaaa tcctgttagt tgatgatgac cgagagctga
    22021 cttccctatt aaaggagctg ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg
    22081 gggaacaggc gcttgatctt ctggacgaca gcattgattt acttttgctt gacgtaatga
    22141 tgccgaagaa aaatggtatc gacacattaa aagcacttcg ccagacacac cagacgcctg
    22201 tcattatgtt gacggcgcgc ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg
    22261 cagatgacta tctcccgaaa ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga
    22321 tcctgcgccg ttcgcactgg agcgagcaac agcaaaacaa cgacaacggt tcaccgacac
    22381 tggaagttga tgccttagtg ctgaatccag gccgtcagga agccagcttc gacgggcaaa
    22441 cgctggagtt aaccggtact gagtttaccc tgctctattt gctggcacag catctgggtc
    22501 aggtggtttc ccgtgaacat ttaagccagg aagtgttggg caaacgcctg acgcctttcg
    22561 accgcgctat tgatatgcac atttccaacc tgcgtcgtaa actgccggat cgtaaagatg
    22621 gtcacccgtg gtttaaaacc ttgcgtggtc gcggctatct gatggtttct gcttcatgat
    22681 aggcagctta accgcgcgca tcttcgccat cttctggctg acgctggcgc tggtgttgat
    22741 gttggttttg atgttaccca agctcgattc acgccagatg accgagcttc tggatagcga
    22801 acagcgtcag gggctgatga ttgagcagca tgtcgaagcg gagctggcga acgatccgcc
    22861 caacgattta atgtggtggc ggcgtctgtt ccgggcgatt gataagtggg caccgccagg
    22921 acagcgtttg ttattggtga ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat
    22981 gcagatcatt cgtaacttta ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa
    23041 gtatggccgc gtggaactgg tcggtccgtt ctccgtgcgt gatggcgaag ataattacca
    23101 actttatctg attcgtccgg ccagcagttc tcaatccgat ttcattaact tactgtttga
    23161 ccgcccgcta ttactgctga ttgtcaccat gttggtcagt acgccgctgc tgttgtggtt
    23221 ggcctggagt ctggcaaaac cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca
    23281 gggaaactta cgccagcacc cggaactgga agcggggcca caggaattcc ttgccgcagg
    23341 tgccagtttt aaccagatgg tcaccgcgct ggagcgcatg atgacctctc agcagcgtct
    23401 gctttctgat atctctcacg agctgcgcac cccgctgacg cgtctgcaac tgggtacggc
    23461 gttactgcgc cgtcgtagcg gtgaaagcaa ggaactcgag cgtattgaaa ccgaagcgca
    23521 acgtctggac agcatgatca acgatctgtt ggtgatgtca cgtaatcagc aaaaaaacgc
    23581 gctggttagc gaaaccatca aagccaacca gttgtggagt gaagtgctgg ataacgcggc
    23641 gttcgaagcc gagcaaatgg gcaagtcgtt gacagttaac ttcccgcctg ggccgtggcc
    23701 gctgtacggc aatccgaacg ccctggaaag tgcgctggaa aacattgttc gtaatgctct
    23761 gcgttattcc catacgaaga ttgaagtggg ctttgcggta gataaagacg gtatcaccat
    23821 tacggtggac gacgatggtc ctggcgttag cccggaagat cgcgaacaga ttttccgtcc
    23881 gttctatcgg accgatgaag cacgcgatcg tgaatctggc ggtacaggat tggggctggc
    23941 gattgttgaa accgccattc agcagcatcg tggctgggtg aaggcagaag acagcccgct
    24001 gggcggttta cggctggtga tttggttgcc gctgtataag cggagttaaa ctccgcattt
    24061 gtaggcagga taaggcgttt acgccgcatc cggcatttga gcaggatgcc tgatgcgacg
    24121 ctgatagcgt cttatcaggc ctacactccg ggggttattt tccccacaac cggcgggaaa
    24181 aatcttcgat cttgccgctc agtcggcgct tttgcatcgt ccgcgtccag cttttcgata
    24241 acccggcggc ggagagtaaa cggtgatact gatcgtcatc aaacggcata tgccatgcga
    24301 tggcggcagc ctcctgcacg gtcacatcgc tgacacggga aaccaactcc agcggcgcgt
    24361 ccgcagacac tttccctggc gctatcacgc tatacaacca ccccacctta cctgtgtttt
    24421 gcatcaactg cgctatatcg ctgatatcaa aatggtaatt gagtttatag caaggcgagc
    24481 gcggctggct gacctgaatt aatgcctctc cccagcggaa aatatcgccc atatgaacat
    24541 tactttccgt gagaccgtcg gttgagagat tttcgccaaa cgcaggcgca acaaacaact
    24601 ccgcctgttc cggaaattcc cgcacccagt agagatagtg ctcacgagga taatgacaca
    24661 gcgctctgtc tggcccgccg tgaacctttt tctccgcctg ctcgtcacct tccagcccca
    24721 gctccgtcag catcaactca ccatcaacct ggattttggc aattgcgctg ggtttgcctt
    24781 cgggataagc ctgaatcttg cctgtgtata catcaaccgg atatcgcatc tgttccctct
    24841 ccacgagaaa tttccccatt agaccatatt tattttatgt caggcgtgct gttttcgcgg
    24901 taagtatcca ggcaggaact caatagcaaa ccggacgaac gttagttcat ccggctgtgg
    24961 ggattaagga agcaggttta tttcaccgta cctaaaattt cgattttcgc tgctctggct
    25021 ttgactttac gtgaccagat agaggtaaga attggcacca gaatcgaagt cacaatgacc
    25081 gccgtcgcta ccagcgaagt tgctgccgga gccatcggtt taaacgcagg caccatttct
    25141 gcaatcagca caggtgtcgc taccgcggcc cctgcggaac tggaagcggc aattccggca
    25201 gtgccatcgc caccgccaat caatttatcg gcgataatca acggaatacc ggtcacgata
    25261 attactgcca cacccaacag gatccccagc aaaccagtct gggcaatcac agtcaaatca
    25321 atggtattgc ccagcgcgaa ggcaaagaat ggaatcagcg tttgcactgc tttgctgaaa
    25381 aattctcgta attcagggtc aagattcccc agggcaaagc ccaccaggaa cggtaatact
    25441 gcgccgacga aaacatgcgg ttcaaacgag gcaatcccgg cagtgcccag aataatcatc
    25501 gtcatgagcg gcccggactc caacgacatc aacacaaatg ccccagcttc ttctttcgta
    25561 ccgtactgct gcatgatgga agcgtaaagt ccgccgttgg tcatatccat cgccgccacc
    25621 agcgccagcg ttgaaagtcc ggcaaagaat ccaacttcaa caccatgttc cggaataatg
    25681 cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt tagttaccac cagcgtaccg
    25741 gatttacgca gtaccgttcc cgtcgcgctt aattttattg acgcccccat gcaaaaaaac
    25801 cacaccgcca gaatgggcac cgtaccggta atcatcccgt tggtgaatga tccaaaatat
    25861 ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa ggaatagcgg gacgagcatc
    25921 atcccccccg ggattttctc aatcgagcgt tttatctgca tttccatcac ctgtcggcat
    25981 atttttaagg gctgatgcgt aattcctcaa tggagcatca gtgttgatgg aaacgaagat
    26041 agacattcat ttaatcaata aaagtgatca aaaccacatc aattgaaacg ctgttttatt
    26101 tttatcggat cattgttttt attttgaaat tgatcacaaa aaaaccaccg ccgttggcga
    26161 tggttcattt tttaagctga tatgcggcct atacgcctca ttgcagcagg cggcaaatga
    26221 ttattttttc gccgcaaaac gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc
    26281 tttaatgtag tccggacggc ggttctggaa tttcaggtag taagcatgtt cccacacatc
    26341 caggcccaga atcgggaagc cggaagcgcc agaaatagct tcacccatca gcggagaatc
    26401 ctggttagca gtagaaacca ccgccagttt atcgcctttc agcaccagcc atgcccagcc
    26461 ggaaccaaag cgggaagctg ccgctttttc aaattctgct ttgaagttat caacggagcc
    26521 gaagtcacgt tcgatagccg ctttcaggtc accctgcagg gtggtgcctt ttttcagacc
    26581 tttccagaac aggctgtggt tagcgtgacc gccagcgttg ttgcgcagta cggttttctt
    26641 gtctgctggc agctggtcca gtttggtgat cagctcttca accggcaggt tggcaaattc
    26701 tggcaggctt tccagcgccg cgttggcgtt gtttacgtag gtctgatggt gtttggtgtg
    26761 gtggatttcc atggtctgct tatcgaagtg cggttccagg gcatcgtaag catacggcag
    26821 ggatggcagg gtatagctca tattcatctc cagtattgtc gggcggccga ttgttaatgc
    26881 cgcgtaagca gttggttcat tatagttaat taaatgatat tgaaaatgat tatcaatgcc
    26941 gtacttttcg taagggtatg gttttgcagg aaaatgcccg agatgtgaag caaatcaccc
    27001 acttaatgcc gtgattgcca gtaaatcgac aacggcggca acaggcgaaa ggttaatcga
    27061 cagcacgatt tttacactca tctcgtcgga gatgtgaagc gacgaaaaat gatgaggata
    27121 agaagatgag taacgcgatt acgatgggga tattttggca tttgatcggc gcggccagtg
    27181 cagcctgttt ttacgctccg ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt
    27241 cagtcggtgg gattgtttcg tggattattc tgccgtggac catcagcgcc ctgttactac
    27301 cggatttctg ggcgtattac agctcgttta gtctctctac gctgctgcct gtttttctgt
    27361 tcggcgctat gtgggggatc ggtaatatca actatggcct gaccatgcgt tatctcggca
    27421 tgtcgatggg aattggcatc gccattggca ttacgttgat tgtcggtacg ctgatgacgc
    27481 caattatcaa cggcaatttc gatgtgctga ttaacaccga aggcggacgc atgacgttgc
    27541 tcggcgttct ggtggcgctg attggcgtag ggattgtaac tcgagccggg cagttgaaag
    27601 agcgcaagat gggcattaaa gccgaagagt tcaatctgaa aaaagggctg gtgctggcgg
    27661 tgatgtgcgg cattttctct gccgggatgt cctttgcgat gaacgccgca aaaccgatgc
    27721 atgaagccgc tgccgcactt ggcgtcgatc cactgtatgt cgctctgcca agctatgttg
    27781 tcatcatggg cggcggcgcg atcattaacc tcggtttctg ttttattcgt ctggcaaaag
    27841 tgaaggattt gtcgctaaaa gccgacttct cgctggcaaa accgctgatc attcacaatg
    27901 tgttactctc ggcactgggc gggttgatgt ggtatctgca attctttttc tatgcctggg
    27961 gccacgcccg cattccggcg cagtatgact acatcagttg gatgctgcat atgagtttct
    28021 atgtattgtg cggcggtatc gtcgggctgg tgctgaaaga gtggaacaat acaggacgcc
    28081 gtccggtaac ggtgttgagc ctcggttgtg tggtgattat tgtcgccgct aacatcgtcg
    28141 gcatcggcat ggcgaattaa tctttctgcg aattgagatg acgccactgg ctgggcgtca
    28201 tcccggtttc ccgggtaaac accaccgaaa aatagttact atcttcaaag ccacattcgg
    28261 tcgaaatatc actgattaac aggcggctat gctggagaag atattgcgca tgacacactc
    28321 tgacctgtcg cagatattga ttgatggtca ttccagtctg ctggcgaaat tgctgacgca
    28381 aaacgcgctc actgcacgat gcctcatcac aaaatttatc cagcgcaaag ggacttttca
    28441 ggctagccgc caaccgggta atcagcttat ccagcaacgt ttcgctggat gttggcggca
    28501 acgaatcact ggtgtaacga tggcgattca gcaacatcac caactgcccg aacagcaact
    28561 cagccatttc gttagcaaac gacacatgct gactactttc atgctcaagc tgaccgataa
    28621 cctgccgcgc ctgcgccatc cccacgctac ctaagcgcca gtgtggttgc cctgcgctgg
    28681 cgctaaatcc cggaatcgct ccctgtcagt caagattcag cttcagccgc tccgggcaat
    28741 aaataatatt ctgcaaaacc agatcgttaa cggaagcgta ggagtgttta tcgtcagcat
    28801 gaatataaaa gagatcgcca cgggtaatgc gataaggacg atcgttgagt acatgcagac
    28861 cattaccgcg ccagacaatc accagctcac aaaaatcatg tgtatgttcg gcaaagacat
    28921 cttgcggata acggtcagcc acagcgactg cctgctggtc gctggcaaaa aaatcatctt
    28981 tgagaagttt taactgatgc gccaccgtgg ctacctcggc cagagaacga agttgattat
    29041 tcgcaatatg gcgtacaaat acgttgagaa gattcgcgtt attgcagaaa gccatcccgt
    29101 ccctggcgaa tatcacgcgg tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg
    29161 ttactgtcgc tgaatccaca gcgataggcg atgtcagtaa cgctggcctc gctgtggcgt
    29221 agcagatgtc gggctttcat cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc
    29281 gtttgctgct taagctgccg atgtagcgta cgcagtgaaa gagaaaattg atccgccacg
    29341 gcatcccaat tcacctcatc ggcaaaatgg tcctccagcc aggccagaag caagttgaga
    29401 cgtgatgcgc tgttttccag gttctcctgc aaactgcttt tacgcagcaa gagcagtaat
    29461 tgcataaaca agatctcgcg actggcggtc gagggtaaat cattttcccc ttcctgctgt
    29521 tccatctgtg caaccagctg tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga
    29581 gacggatact gcccatccag ctcttgtggc agcaactgat tcagcccggc gagaaactga
    29641 aatcgatccg gcgagcgata cagcacattg gtcagacaca gattatcggt atgttcatac
    29701 agatgccgat catgagcgcg tacgaaacag accgtgccac cggtgatggt atagggctgc
    29761 ccattaaaca catgaatacc cgtgccatgt tcgacaatca caatttcatg aaaatcatga
    29821 tgatgttcag gaaaatccgc ctgcgggagc cggggttcta tcgccacgga cgcgttacca
    29881 gacggaaaaa aatccacact atgtaatacg gtcatactgg cctcctgatg tcgtcaacac
    29941 ggcgaaatag taatcacgag gtcaggttct taccttaaat tttcgacgga aaaccacgta
    30001 aaaaacgtcg atttttcaag atacagcgtg aattttcagg aaatgcggtg agcatcacat
    30061 caccacaatg aagctaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
    30121 ccattttcct gtcagtaacg agaaggtcac gaattcaggc gctttttaga ctggtcgtaa
    30181 tgaaattcag caggatcaca ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc
    30241 atccagtggg cgcgtgatgc tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg
    30301 cgaaatccat cgttttaaca atggcctgca tagccagaac ggttatgtca cctggaatgt
    30361 ggatagcctg gaaagtgcca ttcgccttgg attaaacaag gtgtgcgagg aagggattcg
    30421 tatcgatagc attgggattg atacctgggg cgtggacttt gtgctgctcg accaacaggg
    30481 tcagcgtgtg ggcctgcccg ttgcttatcg cgatagccgc tccaatggcc taatggcgca
    30541 ggcacagcaa caactcggca aacgcgatat ttatcaacgt agcggcatcc agtttctgcc
    30601 cttcaatacg ctttatcaac tgcgtgcgct gacggagcaa caacctgaac ttattccaca
    30661 cattgctcac gctctgctga tgccggatta cttcagctat cgcctgaccg gcaagatgaa
    30721 ctgggagtac accaacgcca cgaccacgca actggtcaat atcaatagcg acgactggga
    30781 cgagtcgcta ctggcgtgga gcggggctaa caaagcctgg tttggtcgcc cgacgcatcc
    30841 gggtaatgtc attgggcact ggatttgccc gcagggtaat gagattccgg tggtcgccgt
    30901 tgccagccat gataccgcca gcgcggttat cgcctcgccg ttaaacggtt cacgcgctgc
    30961 ttatctctct tctggcacct ggtcattgat gggcttcgaa agccagacgc catttaccaa
    31021 tgacacggcg ctggcagcca acataaccaa tgaaggcggg gcggaaggtc gctatcgggt
    31081 gctgaaaaat attatgggct tatggctgct tcagcgagtg cttcaggagc ggcaaatcaa
    31141 cgatctcccg gcgcttatcg ccgcgacaca ggcacttccg gcctgccgct tcatcatcaa
    31201 tcccaatgac gatcgcttta ttaatcctga ggcgatgtgc agcgaaattc aggctgcgtg
    31261 tcgggaaatg gcgcaaccgc tcccggaaag tgatgctgaa ctggcgcgct gcattttcga
    31321 cagtctggcg ctgctgtatg ccgatgtgtt gcatgagctg gcgcagctac gcggtgaaga
    31381 tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac acgctgctca accagctatg
    31441 tgccgatgcc tgcggtattc gggtgatcgc cgggcctgtt gaagcctcga cgctcggcaa
    31501 tatcggcatc cagttaatga cgctggatga actcaacaat gtggatgatt tccgtcaggt
    31561 cgtcagcacc accgcgaatc tgaccacctt tacccctaat cctgacagtg aaattgccca
    31621 ctatgtggcg cagcttcact cgacacgaca gacaaaggag ctttgcgcat gaccactcaa
    31681 ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg cggtggggat tgatgtcgag
    31741 gaggcgctgc gccaacttga tcgtttaccc gtttcaatgc actgctggca gggcgatgat
    31801 gtttccggtt ttgaaaaccc ggaaggttcg ctgaccggtg ggattcaggc tattggtaat
    31861 tatccgggca aagcgcgtaa tgccagtgag ctacgagccg atctggaaca ggctatgcgg
    31921 ctgattccgg ggccgaaacg gcttaattta catgccatct atctggaatc agacacgcca
    31981 gtctcgcgcg accagatcaa acctgagcac ttcaaaaact gggttgaatg ggcgaaagcc
    32041 aatcagctcg gtctggattt taacccctcc tgtttctcgc atccgctaag cgccgatggc
    32101 tttacgctct cgcatcccga cgacagcatt cgccagttct ggattgatca ctgcaaagcc
    32161 agccgtcgcg tttcggccta ttttggcgag caactcggca caccatcggt gatgaacatc
    32221 tggatcccgg atggtatgaa agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg
    32281 ctggcagcac tggatgaggt gatcagcgag aagctggatc cggcgcacca tatcgacgcc
    32341 gttgagagca aattgtttgg cattggcgca gagagctaca cggttggctc caatgagttt
    32401 tacatggggt atgccaccag ccgccagact gcgctgtgcc tggacgccgg gcatttccac
    32461 ccgactgaag tgatttccga caagatttcc gccgccatgc tgtatgtacc gcagttgctg
    32521 ctgcacgtca gccgtccggt tcgctgggac agcgatcacg tggtgctgct ggatgatgaa
    32581 acccaggcga ttgccagtga gattgttcgt cacgatctgt tcgaccgggt gcatatcggc
    32641 ctcgacttct ttgatgcctc tatcaaccgc attgccgcgt gggtcattgg tacacgcaat
    32701 atgaaaaaag ccctgctgcg tgcgttgctg gaacctaccg ctgagctgcg caagctggaa
    32761 gcggcgggcg attacactgc gcgtctggcg ctactggaag aacagaaatc gttgccgtgg
    32821 caggcggtct gggaaatgta ttgccaacgt cacgatacgc cagcaggtag cgaatggctg
    32881 gagagcgtgc gggcttatga gaaagcgatt ttgagccagc gtgggtaaac actgccggat
    32941 gcggcgcgag cgccttatcc ggcctacagg tcgaatgacc atgcgtgctg ccaccctcac
    33001 cccggccctc tccctgaaag ggcgagggag aaaaacatag cctcgtgcgg caacggagct
    33061 gaacaccgct ccggacagtc ccctcgcccc tacggggaga gggttagggt gaggggaaca
    33121 agccgcacca tgtctaaaca aggcaaccta atttacagga acacagaaca tgcaaaacat
    33181 tacccagtcc tggtttgtcc agggaatgat caaagccacc accgatgcct ggctgaaagg
    33241 ctgggacgag cgcaatggtg gtaacctgac gctgcgcctg gatgacgccg atatcgcgcc
    33301 ttatcacggc aactttcatg cacaaccgcg ctatattccc ctcagccaac ccatgccttt
    33361 actggcaaat acgccgttta ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct
    33421 tgatcctgca gctaacttag gcgtcgtaaa agtcgacagc gacggcgcgg gctaccatat
    33481 tctctggggg ttaaccaacg aagccgttcc aacttccgaa cttccggctc acttcctttc
    33541 ccactgcgag cgcattaaag ccaccaacgg taaagatcgg gtgatcatgc actgccacgc
    33601 caccaacctg atcgccctca cctatgtact tgaaaacgac accgcggtct ttactcgcca
    33661 actgtgggaa ggcagcaccg agtgtctggt ggtattcccg gatggcgttg gcattctgcc
    33721 gtggatggtg cccggcacgg acgaaatcgg ccaggcgacc gcacaagaga tgcaaaaaca
    33781 ttcgctggtg ttgtggccct tccacggcgt cttcggcagc gggtcgacgt tggatgaaac
    33841 attcggttta atcgacaccg cagaaaaatc agcacaagta ttagtgaagg tttattcgat
    33901 gggcggcatg aaacagacca tcagccgtga agagttgata gcgctcggca agcgcttcgg
    33961 cgttacacca ctagctagtg cgctggcgct gtaaggagca aacatgatcc gcaaagcctt
    34021 tgtcatgcag gttaaccccg acgcccacga agagtatcag cgtcggcata accccatctg
    34081 gccggaactg gaagcagtgt tgaaatctca cggtgcgcat aactacgcca tttacctcga
    34141 caaagcgcgt aatctgctgt ttgccacggt agagattgaa tctgaagaac ggtggaatgc
    34201 ggtagccagc actgatgttt gccaacgttg gtggaaatat atgaccgatg ttatgcccgc
    34261 taacccggat aacagcccgg tgagtagcga gctgcaagaa gtgttttacc tgccgtaatt
    34321 gcccaatgtg ccagagatgc ctccggcaca atattttaaa tttcgatttg aaaaattaaa
    34381 catattacat ctcccggtaa gttatatttc cctgatacat tgtgagtaaa tcacaaaaat
    34441 aatgaataac ccattaatga ttcatgtggt ttatttaaat aaccattatg tgcattactc
    34501 cgcaaatctg acctttcact ctgtctcaaa ttgtcattgt ttaccgacat atcgcctctc
    34561 atcacttaat cgctccacca ttacaattga ttaaaatata ttttatggag tccgattaat
    34621 ggcagctctt actgcaagct gtattgacct gaatattcag ggcaatggcg cttattccgt
    34681 tctgaagcag ttggcgacaa tagcgttaca aaacggtttt atcaccgact cacaccagtt
    34741 cctgcaaacc ctgctgctgc gcgaaaaaat gcactctact ggatttggtt ccggtgtcgc
    34801 cgtgccgcac ggtaaaagcg cctgcgttaa acaaccgttt gtattattcg cccgcaaagc
    34861 gcaggctatt gactggaaag ccagcgatgg cgaagacgtc aattgctgga tctgcctcgg
    34921 cgtgccgcaa agcggcgaag aggatcaggt caaaatcatc ggcacactgt gtcgcaaaat
    34981 tatccaccag gattttattc atcaactgca acagggcgac gccgaccagg tgcttgcctt
    35041 gttaaatcaa accctcagct cataaggaag tggcgatgga gtcatcctta tgtattgtcg
    35101 cgatcaccaa ctgccccgcc gggatcgctc acacctacat ggtggcggaa gccctggaac
    35161 agaaagcccg ttctctcggc cataccataa aagtggaaac tcaagggtcc agtggcgttg
    35221 aaaaccgctt atccagcgaa gagattgccg ctgccgatta cgtcattctc gctaccgggc
    35281 gtggcctgag cggtgatgat cgcgcgcggt ttgccgggaa gaaagtttat gagattgcca
    35341 tctcccaggc gttgaaaaat atcgaccaga ttttcagcga attaccgaca aactcgcagc
    35401 tttttgccgc agatagcggc gtgaagcttg gcaaacagga agtgcaaagc ggcagcgtga
    35461 tgagccacct gatggcgggc gtctctgcgg cgctgccgtt tgtcatcggc ggtggtatcc
    35521 tggtggcgct tgccaacatg ctggtgcagt tcggtttgcc gtataccgat atgtcgaaag
    35581 gcgcgccgtc gtttacctgg gtagttgaat ccatcggtta ccttggcttc acctttatga
    35641 tccccatcat gggcgcttat attgcctcgt cgattgccga taaaccggct ttcgctccgg
    35701 cgtttctggt ttgctatctg gcgaacgaca aagcgctgct cggcacccag tcgggtgcgg
    35761 gattcctcgg cgcggtggtg tttctggttt gctatctggc gaacgacaaa gcgctgctcg
    35821 gcacccagtc gggtgcggga ttcctcggcg cggtggtgct ggggctggcg attggctatt
    35881 tcgtcttctg gttccgcaaa gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa
    35941 tgctgatccc gttcgttact ctgctggttt tcggcgtgct cacttactac gttatcgggc
    36001 cggtgatgtc cgacctcatg ggcgggctgc tccacttcct gaataccatt cctccatcaa
    36061 tgaagtttgc ggcagcgttt ctggtcggtg cgatgctggc gttcgatatg ggcggcccca
    36121 tcaataaaac cgcctggttc ttctgcttct cactgctgga aaaacacatt tatgactggt
    36181 acgccatcgt cggcgttgtc gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca
    36241 ttgcgcccaa actgtttacc cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg
    36301 gtgccacagt cgcaaccgaa ccggctatac cttacgctct ggcagctccg ttgccgatga
    36361 ttaccgccaa tacgctggcg ggtggcatta ccggcgtgct ggtgatcgct ttcggaatca
    36421 aacgtctggc acctggtttg ggtatctttg acccgctgat cggcctgatg tcgccggtgg
    36481 gttcgtttta tctggtgcta gcgattggcc tcgcgctgaa catttcattc attatcgtcc
    36541 tgaaaggatt gtggttacgc cgcaaagcga aagccgcgca acaggagctt gtccatgaac
    36601 attgagttac tgcaacagtt gtgcgaagcc agcgccgtca gcggcgatga acaggaagtt
    36661 cgcgacattc tgataaacac gctggaacct tgcgttaatg agatcacctt tgatggtctg
    36721 ggcagctttg ttgcccgtaa ggggaataaa ggtccaaaag ttgccgttgt caggcatatg
    36781 gatgaagtcg gctttatggt cacccacatc gacgagagcg gttttctgcg ctttaccacc
    36841 attggcggct ggtggaatca gtcaatgctc aaccaccggg taaccatacg cacacacaag
    36901 ggatttaaaa tccctggtgt gattggttcc gtcgcacctc atgcgttaac ggaaaagcaa
    36961 aagcaacagc cgctgtcatt tgatgagatg ttcattgata ttggcgcgaa cagtcgcgat
    37021 gaggtggaaa aacgcggtgt ggcgatgggc gattttatta gcccggaagc caattttgcc
    37081 tgctggggcg aagataaagt ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg
    37141 atggctgaac tattgcagac ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc
    37201 gtggaagaag aagttgggct acgcggggca caaacctcgg cggaacacat taaaccggat
    37261 gtggtgattg tgctggatac cgctgtcgca ggtgatgttc cgggcattga taacattaaa
    37321 tacccgctga aactgggcca ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc
    37381 aaccagaaac tggtggcggc gttaaaaagc tgtgccgcac ataacgattt accgctgcaa
    37441 ttttccacca tgaaaaccgg tgcgacggat ggcgggcgct acaacgtgat gggcggcggg
    37501 cgtccggttg ttgcgctgtg cctgccaacc cgctatctgc acgctaacag cggcatgatt
    37561 tcaaaagccg attacgatgc tctgctcacg ttgatacggg attttctgac caccttaacc
    37621 gcggagaaag tcaacgcgtt tagccagttc cgtcaggtgg attaatgcta aacgaacgcc
    37681 agttaaagat tgtcgatctg ctggagcaac agccgcgcac gcctggtgag ctggcgcaac
    37741 agactggcgt ttcaggcagg accatcctgc gtgatattga ctatctcaac tttaccctta
    37801 acggcaaagc ccgcattttc gccagtggca gtgcgggcta tcagctggaa atcttcgagc
    37861 gccgcagctt ttttcagttg ctgcaaaagc acgataacga cgatcggctg ctggcgctgt
    37921 tattactgaa tacctttact ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa
    37981 cctgggtagc agagcgtctg ccccggttaa aacagcgtta tgaacgcact tgttgcctgg
    38041 ccagtcgccc cggtttgggc catttcattg atgagacaga agagaaacgc gttatcttgc
    38101 tggcgaactt gctgcgcaaa gatccgtttt taattccgct ggcgggcgta acacgagaca
    38161 attttcagca tttatccacg gcctgcgaca accaacaccg ctggccgctc atgcagggtg
    38221 attatctctc cagcctgatt ctggcgattt acgccctgcg taatcaactg accgatgagt
    38281 ggccgcaata tcccggtaac gagataaaac aaatcgttga acagagcggc atgtttcttg
    38341 gtgataacgc tgtaagaacc ctgacaggtt tgatagagaa acagcatcag caagcgcagg
    38401 taatttcagc cgatcatgtg ctggggttgc tgcaaagggt gccaggcatc gcgtcattga
    38461 atattattga tacgcagctg gttgagaata ttaccgggca tttattacgt tgccttgccg
    38521 caccggtgtg gattgccgag caccgccaaa gcagcatgaa taacctgaaa gccgcctggc
    38581 ctgcggcctt tgatatgagt ctgcacttta ttacgctact gcgtgaacag ctcgatattc
    38641 cccttttcga tagcgatctg atcggtttgt attttgcctg tgcgctggaa cggcatcaaa
    38701 acgaacgcca gccgatcatt ttgctctcgg accagaacgc gattgccact attaatcagc
    38761 tcgccattga gcgcgatgtt ttacattgtc gggtgattat tgcccgtagc ttaagcgagc
    38821 ttattgccat tcgcgaagag attgagccgt tattgatcat taacaacagc cattatttac
    38881 tggatgacgc ggtaaataat tacatcaccg taaaaaatat cattacggct gccgatatcg
    38941 aacaaataaa acactttctg gcgacggcat ttattcgcca acagccggaa cgctttttct
    39001 ctgcccccgg aagttttcat tattcgaatg tacgcggtga aagctggcaa catattaccc
    39061 ggcaaatttg tgcgcaatta gtggcacaac accatattac cgccgatgaa gcgcaacgga
    39121 tcatcgcccg cgaaggcgaa ggtgaaaacc tgattgttaa tcgcctcgcc atcccacatt
    39181 gctggagcga acaggagcga cgttttcgtg gattttttat taccctcgcc caaccagttg
    39241 aggtgaataa cgaagtcatt aaccatgtct tgatcgcctg cgccgccgcc gatgcgcgtc
    39301 atgagctaaa aatatttagc tatctggcaa gcgtattgtg tcagcatccg gcagaagtta
    39361 ttgccgggtt aacaggatat gaggcattta tggagttact tcacaagggg tgaactgtgt
    39421 cggatgcgcc gcatccgaca ctgcattttc atcaaaaacg tttaacgcag gctattatag
    39481 tcatcaacca tttcgttgta ctcgcgtaac gctcgtggaa atgagtcttc gaccatccag
    39541 cttgtgtttg catcctgaag atgctcttta tcccagtcag agtacgggac tttatctctg
    39601 acgcggcgaa catatttttt agtctcgagt tgatactggt ctgccgaatt aatgaacgag
    39661 aaattcatac cgcctttatc cgcttctttg gcctgcgcta ccagcgtttc cagttcagag
    39721 actttcttca ttgccgcatc aacatcaa
//
LOCUS       DAZPJR010000017        39409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPJR010000017 DAZPJR010000000
VERSION     DAZPJR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.018"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHVB7_000875"
     CDS             <1..266
                     /locus_tag="ACHVB7_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR1912941.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHVB7_000876"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHVB7_000876"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR1912942.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHVB7_000877"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHVB7_000877"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR1912943.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHVB7_000878"
     CDS             complement(1570..3270)
                     /locus_tag="ACHVB7_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR1912944.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQEQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHVB7_000879"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHVB7_000879"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR1912945.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHVB7_000880"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHVB7_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR1912946.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="ACHVB7_000881"
     CDS             4828..6600
                     /locus_tag="ACHVB7_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1912947.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHVB7_000882"
     CDS             6593..8374
                     /locus_tag="ACHVB7_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1912948.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHVB7_000883"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHVB7_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR1912949.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHVB7_000884"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHVB7_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR1912950.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHVB7_000885"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHVB7_000885"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR1912951.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHVB7_000886"
     CDS             11336..11908
                     /locus_tag="ACHVB7_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR1912952.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHVB7_000887"
     CDS             complement(11939..12250)
                     /locus_tag="ACHVB7_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR1912953.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHVB7_000888"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHVB7_000888"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHVB7_000889"
     CDS             12629..12982
                     /locus_tag="ACHVB7_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR1912954.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHVB7_000890"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHVB7_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHVB7_000891"
     CDS             complement(14736..15206)
                     /locus_tag="ACHVB7_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR1912955.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHVB7_000892"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHVB7_000892"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR1912956.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHVB7_000893"
     CDS             complement(16044..16262)
                     /locus_tag="ACHVB7_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR1912957.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHVB7_000894"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHVB7_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR1912958.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /gene="acrB"
                     /locus_tag="ACHVB7_000895"
     CDS             complement(17208..20357)
                     /gene="acrB"
                     /locus_tag="ACHVB7_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR1912959.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="ACHVB7_000896"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="ACHVB7_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR1912960.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="ACHVB7_000897"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="ACHVB7_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR1912961.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="ACHVB7_000898"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="ACHVB7_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR1912962.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            25913..26011
                     /locus_tag="ACHVB7_000899"
                     /pseudo
     CDS             25913..26011
                     /locus_tag="ACHVB7_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="ACHVB7_000900"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="ACHVB7_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR1912963.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="ACHVB7_000901"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="ACHVB7_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR1912964.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="ACHVB7_000902"
     CDS             26836..27213
                     /locus_tag="ACHVB7_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR1912965.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="ACHVB7_000903"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="ACHVB7_000903"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR1912966.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="ACHVB7_000904"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="ACHVB7_000904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR1912967.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="ACHVB7_000904"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="ACHVB7_000905"
     CDS             30024..30353
                     /locus_tag="ACHVB7_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR1912968.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="ACHVB7_000906"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="ACHVB7_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR1912969.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="ACHVB7_000907"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="ACHVB7_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR1912970.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="ACHVB7_000908"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="ACHVB7_000908"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR1912971.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="ACHVB7_000909"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="ACHVB7_000909"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR1912972.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="ACHVB7_000910"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="ACHVB7_000910"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR1912973.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="ACHVB7_000911"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="ACHVB7_000911"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR1912974.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /gene="ybaL"
                     /locus_tag="ACHVB7_000912"
     CDS             complement(37406..39082)
                     /gene="ybaL"
                     /locus_tag="ACHVB7_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR1912975.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(39320..>39409)
                     /locus_tag="ACHVB7_000913"
     CDS             complement(39320..>39409)
                     /locus_tag="ACHVB7_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1912976.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         9526 a        10049 c        10417 g         9417 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc tcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgatttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgct
//
LOCUS       DAZPJR010000018        39258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPJR010000018 DAZPJR010000000
VERSION     DAZPJR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.019"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHVB7_000914"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHVB7_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR1912977.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHVB7_000915"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHVB7_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR1912978.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHVB7_000916"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHVB7_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR1912979.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHVB7_000917"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHVB7_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR1912980.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHVB7_000918"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHVB7_000918"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR1912981.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHVB7_000919"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHVB7_000919"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR1912982.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHVB7_000920"
     CDS             complement(6574..7338)
                     /locus_tag="ACHVB7_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1912983.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHVB7_000921"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHVB7_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR1912984.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHVB7_000922"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHVB7_000922"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR1912985.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHVB7_000923"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHVB7_000923"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR1912986.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="ACHVB7_000924"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="ACHVB7_000924"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR1912987.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="ACHVB7_000925"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="ACHVB7_000925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR1912988.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="ACHVB7_000926"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="ACHVB7_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR1912989.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="ACHVB7_000927"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="ACHVB7_000927"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR1912990.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHVB7_000928"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHVB7_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR1912991.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="ACHVB7_000929"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="ACHVB7_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR1912992.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="ACHVB7_000930"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="ACHVB7_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR1912993.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHVB7_000931"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHVB7_000931"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR1912994.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALREYVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHVB7_000932"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHVB7_000932"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR1912995.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="ACHVB7_000933"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="ACHVB7_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR1912996.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="ACHVB7_000934"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="ACHVB7_000934"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR1912997.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHVB7_000935"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHVB7_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR1912998.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHVB7_000936"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHVB7_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR1912999.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHVB7_000937"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHVB7_000937"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR1913000.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="ACHVB7_000938"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="ACHVB7_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHVB7_000939"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHVB7_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR1913001.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="ACHVB7_000940"
     CDS             complement(31717..33003)
                     /locus_tag="ACHVB7_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1913002.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="ACHVB7_000941"
     CDS             complement(33054..33995)
                     /locus_tag="ACHVB7_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1913003.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="ACHVB7_000942"
     CDS             complement(34010..34780)
                     /locus_tag="ACHVB7_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR1913004.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="ACHVB7_000943"
                     /pseudo
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="ACHVB7_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="ACHVB7_000944"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="ACHVB7_000944"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR1913005.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39258
                     /gene="caiB"
                     /locus_tag="ACHVB7_000945"
                     /pseudo
     CDS             38069..39258
                     /gene="caiB"
                     /locus_tag="ACHVB7_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9397 a        10789 c        10279 g         8793 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tatgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcctg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctaggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacactggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cactacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattgac ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    39121 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    39181 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    39241 cattcaggag ttggtcag
//
LOCUS       DAZPJR010000019        38611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPJR010000019 DAZPJR010000000
VERSION     DAZPJR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.020"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..673)
                     /gene="focB"
                     /locus_tag="ACHVB7_000946"
     CDS             complement(<1..673)
                     /gene="focB"
                     /locus_tag="ACHVB7_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR1913006.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="ACHVB7_000947"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="ACHVB7_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR1913007.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="ACHVB7_000948"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="ACHVB7_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR1913008.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="ACHVB7_000949"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="ACHVB7_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR1913009.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="ACHVB7_000950"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="ACHVB7_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR1913010.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="ACHVB7_000951"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="ACHVB7_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR1913011.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="ACHVB7_000952"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="ACHVB7_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR1913012.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="ACHVB7_000953"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="ACHVB7_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR1913013.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9837)
                     /gene="hyfD"
                     /locus_tag="ACHVB7_000954"
                     /pseudo
     CDS             complement(8399..9837)
                     /gene="hyfD"
                     /locus_tag="ACHVB7_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="ACHVB7_000955"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="ACHVB7_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR1913014.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="ACHVB7_000956"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="ACHVB7_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR1913015.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="ACHVB7_000957"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="ACHVB7_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR1913016.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="ACHVB7_000958"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="ACHVB7_000958"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR1913017.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="ACHVB7_000959"
     CDS             complement(14170..14742)
                     /locus_tag="ACHVB7_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR1913018.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="ACHVB7_000960"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="ACHVB7_000960"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR1913019.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="ACHVB7_000961"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="ACHVB7_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR1913020.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="ACHVB7_000962"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="ACHVB7_000962"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR1913021.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="ACHVB7_000963"
     CDS             17911..18759
                     /locus_tag="ACHVB7_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR1913022.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /gene="tmcA"
                     /locus_tag="ACHVB7_000964"
     CDS             18774..20789
                     /gene="tmcA"
                     /locus_tag="ACHVB7_000964"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR1913023.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="ACHVB7_000965"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="ACHVB7_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1913024.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="ACHVB7_000966"
     CDS             complement(21671..21871)
                     /locus_tag="ACHVB7_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR1913025.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="ACHVB7_000967"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="ACHVB7_000967"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR1913026.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="ACHVB7_000968"
     CDS             complement(23030..23386)
                     /locus_tag="ACHVB7_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR1913027.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23768..23827
                     /gene="ypfM"
                     /locus_tag="ACHVB7_000969"
     CDS             23768..23827
                     /gene="ypfM"
                     /locus_tag="ACHVB7_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR1913028.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="ACHVB7_000970"
     CDS             complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="ACHVB7_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR1913029.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="ACHVB7_000971"
     CDS             complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="ACHVB7_000971"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR1913030.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSPPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29109..31088
                     /gene="aegA"
                     /locus_tag="ACHVB7_000972"
     CDS             29109..31088
                     /gene="aegA"
                     /locus_tag="ACHVB7_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR1913031.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31156..31731
                     /gene="nudK"
                     /locus_tag="ACHVB7_000973"
     CDS             31156..31731
                     /gene="nudK"
                     /locus_tag="ACHVB7_000973"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR1913032.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31857..32900
                     /locus_tag="ACHVB7_000974"
     CDS             31857..32900
                     /locus_tag="ACHVB7_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR1913033.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="ACHVB7_000975"
     CDS             complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="ACHVB7_000975"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1913034.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35019..35969)
                     /gene="tal"
                     /locus_tag="ACHVB7_000976"
     CDS             complement(35019..35969)
                     /gene="tal"
                     /locus_tag="ACHVB7_000976"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1913035.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36258..38537
                     /gene="maeB"
                     /locus_tag="ACHVB7_000977"
     CDS             36258..38537
                     /gene="maeB"
                     /locus_tag="ACHVB7_000977"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR1913036.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT         9517 a        10172 c        10277 g         8645 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tagccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc
    23461 aggaaatata acattatcga agtattcaaa ttaacaacta ttaaataata ttaaacattt
    23521 ttaacgtaaa cattaagaat attcatcttg ttatctgtct gtattttaac ctgaattaaa
    23581 tcagtattag atgccgtatg aatatctcga aatgttgctg tgtatcacat acaaatcatc
    23641 accgcaggac catagtattt acataagatt tgtccccccg gaggtagtca tcgcagcaat
    23701 cgagtaaatt agcttcacca cggtatattg tttccgtaga atacccataa ttataagaga
    23761 ggttgttatg attgaacgtg aactggggaa ctggaaagac tttatcgaag ttatgcttcg
    23821 taagtaattc ctgagacaaa gtataaacag caagaacccg ctgagcaggt tcttaatcgg
    23881 aaagttgaaa aaggcgacac attggcatgt cgcctttttt attgcttatt ccgggcgcgg
    23941 ctttagcggg aagcggcggc gcaccagcac aaagaacagc ggcacgaagt aaatagccag
    24001 aatagtggct gaaatcatcc cgcccattac gccagtaccc accgcatgct gaccaccgga
    24061 accggcaccc gtgctggttg ccattggcaa tacgccgaag ataaatgcca gcgaggtcat
    24121 cagaatcggg cgtaaacgct gacggcaggc gtggagcgtc gcttcaaaca ggtcgtggcc
    24181 tttttggttc atctcgttag caaactcgac gatcaggatg gcgtttttcg ccgataaacc
    24241 aatgaccgtt aacaggccca cctggaagta aacgtcgttt tccagcccgc gcatccaggt
    24301 tgccagcagc gcgccgatta cccccagcgg cacgaccagc attaccgaga acggcaccga
    24361 ccagctttca tacaacgcag ccagacacag gaataccacc agcaaggaaa tggcgtacag
    24421 cgccggagcc tgcgcgccgg aaagtcgctc ctgatacgac atcgccgtcc actccagacc
    24481 aaagccgttt ggcagctgct tcactaacga ttccataata tccatcgccg taccggtact
    24541 gacccccggt gcggcttccc caacaatctc aaccgcagaa taaccgttat agcgttccag
    24601 acgcggcgag cctgtttccc agcgtgaggt cgcgaaagca gagaagggca ccatgccgcc
    24661 atctttattt cggacatacc agagattgat gtcatctggc agcatgcgat acggcgcagc
    24721 tgcctgcaca tagactttct tcacgcgacc gcgatccata aagtcattca catagctcga
    24781 accccaggcg gtttgcagtg tgtcgttaat atcgtcgata gcaacgccca gcgcctgagc
    24841 tttacgctgg tcgatatcaa tctgcaactg cggactgtcg tcgaggccgt tatggcgcac
    24901 acgggttagc tccgggtttt ccgccgccag cgccagcaac tggttacgtg ctgccatcag
    24961 cgcatcgtga cctgctccag cgtggtcctg caactccata tcaaaacccg cagaactacc
    25021 aagaccgcta attgctggcg ggctgctggc gataacgcga gcttctttaa tcttgttaaa
    25081 cgcctttgtt gcacgctcga taatggcaaa cgaggtgccg gtcttactgt cgcgttcgct
    25141 ccagtctttc aggcggataa acattcgcgc cacgttttgc ccgttacccc cagggccaga
    25201 accaacggtg gcaaacaccg acatgatgtt gtctttttca tgggtgaagt aatatttctc
    25261 gatttgctca acgactttca gggtctgttg ttgggttgaa ccgctgggca attgtaccga
    25321 ggtagtaaac atgccacggt cttccagcgg taagaacgac gtcggcaaac gcaggaacag
    25381 gaacaccatg ccgccaagca gcaggacata aatcacaatc cagcgcaggc tgcggtggag
    25441 aattttcgcc accccttttt cgtagcgttc ggcgttgcgg ttaaacatct ggttaaacca
    25501 ggcaaaaaag cctttttgcc cgtgatgctc accttttttc agtggcttaa gtagtgtggc
    25561 acacagagcc ggagtgagga tcatcgctac cagtactgac agcaccatcg ccgcaacaat
    25621 ggtaatagag aactggcgat agatggcacc ggtggtgccg ccgaagaagg ccattggtac
    25681 aaataccgcc gaaagcacca tcgcaatccc gaccagtgcc ccctggatct gccccatcga
    25741 tttacgtgtg gcttcgcgag gagtgagtcc ttcctcactc ataatacgtt cgacgttttc
    25801 caccaccacg attgcgtcat ccaccagcag accgatcgcc agtaccatcg cgaacatggt
    25861 taaggtgttg acgctgtaac cgaaggcgta aagtacggag aaggttccca tcaacaccac
    25921 cggcacggcg atagtgggta tcagcgtggc gcggaagttt tgcaggaaca gatacataac
    25981 gaggaaaacc agggcgatag cttccagcag cgttttcacc acgtcttcaa tggaggcttt
    26041 aacaaacgag gtggtttcat acgccacctt gtattccagt ccgtgcggga agtactgcgc
    26101 cagctcgtcg agacgattga gcaccagctc cgctgtcgcc atttcgtttg cgccggaggc
    26161 cagttttacc cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt
    26221 ctccgccccc atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcttgatt
    26281 cacccgcaag gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat
    26341 ggtggcgttg agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat
    26401 ctgcgcgttc tgtgactcaa tggcatcagt gacatcttta gccgtcatct ggaaactgtt
    26461 gagtttcgcc gggtccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc
    26521 cccgacgcca ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat
    26581 atcctgttta tccatcgaac catcggtaga gacgaaagca atggtcagaa tattggtatc
    26641 gccggtttta cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc
    26701 tgattgcagc tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa
    26761 acttaaagtg acagatgcct gaccgatgcc actgctctga gatgacatat acatgagatt
    26821 atcgaggccg gtcatatttt gctcgataac ctgggtcacg gtgttttcca gcgtctgggc
    26881 cgatgcgccg ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg
    26941 ttcaacgggc aatgaaaaaa tcgccagggt ccctgtcaga cacaacagga ttgccagcac
    27001 ccaggcaaaa atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc
    27061 tgcgtatcgt tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg
    27121 agaaaaaaag gagttaatgt aaattatcat gcccttttat actgaacctt aagtgcaagt
    27181 attctttggt cagtaggagg cattacatca actgactttc ctcaccctcc gcagagcgaa
    27241 agctaatact cactaacgtg ccgccgccgg aaggtggtga aaaagtcagc gtcccaccca
    27301 gccgttccgc gcgttcgcgc atgatattca gaccataatg accttcgggt tctttcggtt
    27361 caccgatacc aatcccgtta tcacggatat aaaccgtgtg attgccgtcc ggcgcggtga
    27421 cgcaactgac ggcgatttcg ctggcgttgg cgtgcttcat cgcattcagc accgcttcgc
    27481 gaataatttg caacaaatgc acctgcattt gcgcatccag cgccagggtt ggcagacggc
    27541 agtcgagggt cagtttggcg ctggtttgat tttgtaacgt atccagcatt tccctcaacg
    27601 cggaggggag atccgcctgc tgcagcgtca ggcggaaagt agtcaacagc tcgcgtaact
    27661 gccgataagc atcattcaac gcctgggaaa aatcggccat gatactttgt gcggtggcgt
    27721 tatcttccgg tatcgaacgc ttcagtaacg tcaactggat acgtaagtaa gaaagtacct
    27781 gagccagcga gtcgtgcaat tcgcgggcga tggttgcacg ttcttccatc aacaataact
    27841 gctgaaaatg cttttgcgcc tgattaaagt acaaaccgcg tcccagcatc gacgaaacgc
    27901 tgttaagcag cggttctgat gatgaaacgt gactattttg ccagtgcagt tcgccgtaaa
    27961 ccgtctcttg cattgtcacc ggtaaaatct gcatcggcaa ttccgggttt ggttgccctt
    28021 cgctaatccg ccagttttca ccgacattta actccagata ttcagccgct tcattgtcgc
    28081 gaacaatctg caaaatatgg cggaaacaat gcacatcaat ctggcttgtg ttcagcgcct
    28141 gcgaacattg atacaacacc tccagacgac gcttggcctc gtggagatcg cgggtctttt
    28201 cttctactga cgcctccagc gaaaggtaca atttatgcag ctcgctcgac atctggttaa
    28261 aggtttttgc aagcagacca agctcattcg gcagactggt atccagcggc ggcgagtcga
    28321 actgcccgtg ttcaatacgt tgactggcgg taaccagctg attcagcggg gcaaccacct
    28381 gatggcgtat acggcgcaaa gtaaaaaaga ccagcgtgaa aataccgata ccgccagcca
    28441 gggaaatcgc caccaccagc agcattttgc gttcagcgta gtgctgtaaa gccagcacga
    28501 acaggtctat ctgattaaca taattattaa tattggcctg ataccacggc aaatcgccct
    28561 tgctgagccg attattcatc tccagccagt tggcattcag atgcgcatag cgagttttta
    28621 ctgcttctgg cacataccag acgttgagat tggttaatac cgatgaatgc agtgcctgct
    28681 gaaacaactg gcgatgtgca ttgagttgtg gactgccact ttgcaagtcg tagcccaggc
    28741 ggtaactctg catacgcagc gatccggcaa tattgatagc ctcagcgtcg cgcaaactgc
    28801 tcgccagagt tagcagagcg atacccgtgg aaagaatcga cagcagcaca atgtaaaaaa
    28861 aggcccgggc cagactggcc gagacgggtc gtttaacaat cacacgcgtc ttctccacaa
    28921 aaaatcatta atgttccagt tcgctaaaac gcaatttagt cagtgatggg attatttgat
    28981 ctgccacagt ttatagcgaa atagttactt attctgggca catgaacatt gccgattccc
    29041 ctgtaagtcg ggtaataaca acactcatat aaagaataag gtttttacaa ccaaaaaaga
    29101 aggtcgttat gaatcgtttt attatggcca acagtcagca atgtctgggt tgtcatgctt
    29161 gtgaaatcgc ctgtgtcatg gctcacaatg atgagcaaca tgtcctgagc caacaccatt
    29221 ttcatccccg aattacggtt atcaaacatc agcagcaacg tagtgcagtg acctgtcacc
    29281 attgtgaaga tgcgccctgc gcccgtagct gccctaatgg cgcaatcagc cacgttgatg
    29341 acagcattca ggtcaatcag caaaagtgta ttggctgtaa atcctgcgtg gtggcctgtc
    29401 cgtttggtac gatgcaaatc gtcctgacac ccgtcgcggc aggaaaagta aaagccacgg
    29461 cgcataaatg cgacctttgt gcagggcgcg aaaacggtcc tgcctgtgtc gagaattgcc
    29521 cggcggacgc gctgcaactg gtcactgacg tcgcactctc cggcatggcg aaatcccgcc
    29581 gcttgcgcac cgcacgtcag gagcatcaac cgtggcatgc cagtaccgct gcacaagaaa
    29641 tgccggtaat gagtaaagtc gaacaaatgc aggcaacgcc cgcgcgtggc gagccggata
    29701 aactggcgat tgaagcgcgc aaaaccggtt ttgatgaaat ttatctgcca ttccgtgccg
    29761 atcaggcaca acgggaagcc tcgcgctgcc ttaaatgcgg cgagcacagc gtttgtgaat
    29821 ggacctgccc gctgcataac catattccgc agtggattga actggtgaaa gccggaaaca
    29881 tcgacgccgc cgtcgagctt tctcacctga ccaacaccct gccggaaatt accggacgcg
    29941 tttgtccgca ggaccgtttg tgtgaaggtg cctgtactat tcgcgatgag cacggcgcgg
    30001 taactatcgg caacattgaa cgctacattt cagatcaggc gttggcgaaa ggttggcgtc
    30061 ctgacttaag ccatgtcacc aaagtggaca agcgggtggc gattatcggt gcgggtccgg
    30121 cagggctggc ctgtgcggat gttctgaccc gcaatggcgt ggcggtgacg gtgtacgatc
    30181 gccatccaga aattggtggc ttgctcactt tcggcattcc ttctttcaaa ctggataaat
    30241 ccctgctggc acgccgtcgg gaaatcttca gcgcgatggg gattcacttc gaactcaatt
    30301 gtgaagtggg taaagatgtc tctttggatt cgcttttgga acaatacgac gcggtcttcg
    30361 ttggcgtagg cacttaccgt tccatgaaag cgggtttacc caatgaagat gcgccgggcg
    30421 tttatgacgc gctgccgttc ctcatcgcca acactaaaca ggtgatggga ctcgaagagc
    30481 taccggaaga gccgtttatc aataccgccg gactcaacgt cgtggtactg ggcggcggcg
    30541 acaccgcgat ggactgtgtg cgtaccgcgc tgcgccacgg cgcgagtaac gtcacctgcg
    30601 cttatcgtcg tgatgaagct aacatgccag gctcgaagaa agaggtgaag aacgcccgcg
    30661 aagagggggc caacttcgaa tttaacgtcc agccggtggc gcttgagctg aatgagcaag
    30721 gtcacgtttg cggaattcgt ttcctgcgca cgcgtcttgg cgagccggat gcccaggggc
    30781 gccggcgact tgtaccgatt gagggcagtg aatttgtcat gccagccgat gcggtgatta
    30841 tggtgtttgg cttcaatcca cacgggatgc cgtggctgga gtcgcacggt gtaacggtag
    30901 acaaatgggg ccgcatcatc gcggatgtgg aaagccagta ccgttaccag accaccaatc
    30961 cgaaaatctt cgccggtggc gacgccgtgc gtggtgcgga tctggtggtt accgcaatgg
    31021 cagaaggacg tcatgcggca caggggatta ttgactggct gggggtaaaa tcagtcaaat
    31081 ctcactgata gcctgcgcag acaaacccga cttcacagcg taatataatt gttcatttcg
    31141 cgctgtggag tcggtatgac gcaacaaatc accctcatta aagacaaaat tctctccgat
    31201 aactatttca ccctgcacaa cattacttac gatctcacgc gcaaagatgg cgaagttatc
    31261 cgccataaac gtgaagttta cgatcgcggt aatggtgcga cgatcctcct gtacaacgcg
    31321 aagaaaaaga ccgtggttct gattcgtcag ttccgtgtcg ctacctgggt taatggcaat
    31381 gaaagcgggc agctgattga aacctgcgcc gggctgctgg ataacgacga accggaagtg
    31441 tgcattcgta aagaagcgat tgaagagacg ggctatgaag tgggcgaagt gcgcaaatta
    31501 tttgaactgt atatgtcgcc tggcggtgtg actgagctaa tccacttttt tatcgccgaa
    31561 tacagtgaca atcagcgcgc taacgccggt ggcggtgttg aagatgaaga tattgaagtg
    31621 cttgagctgc cgttcagcca ggcgctggag atgatcaaaa ccggcgagat acgtgacggt
    31681 aagacggtgt tattgcttaa ctatttgcaa acgtcacatt taatggactg aaaaataaca
    31741 ataatatttc gttgtttatt attggctcaa tccgacaaat ctgattgagc cgcgctacct
    31801 gcgcaacgaa gatacgactt gtgctgtttg tttgaacttc tggggtcgta ccgtccatgc
    31861 gctatcgcat tttccttctc ttttttttcg ctttgttgcc gacgtctttg gtgtgggcgg
    31921 cactagcgca acgggcgttt tccgactggc aggtcacctg taataaccaa aacttctgcg
    31981 tggcgcgcaa tacgggcgat cataatggac tggtgatgac cctgagccgc agcgccgggg
    32041 cgcataccga tgccgtttta cgtattgagc gcggcgggtt gaagtcgccg gatgcgtctg
    32101 aaggagagat agcgccacgg ctgctgttag atggtgagcc gttggcgtta agtggtgaca
    32161 agtggcggat ttcaccatgg ttattagtaa ccgatgatac ggcaaccatc accgcgtttt
    32221 tgcagatgat tcaggaaggg aaggcgatca ccttacgcga cggcaatcag accatttctc
    32281 tgagtggctt aaaagcggcg ttgttgttta ttgatgctca gcaaaagcgc gttggcagtg
    32341 aaaccgcgtg gatcaagaaa ggggacgaac cgccgctcag cgtaccgccc gcgcctgcgc
    32401 tgaaagaggt cgcggtggtt aacccaacgc cgacgccact ctcactcgaa gaacgcaacg
    32461 atttgctgga ttatggaaac tggcggatga atggtctgcg ctgctcgctt gatccgttac
    32521 gtcgtgaggt gaatgtcact gcgctgactg atgataaagc gctgatgatg atcagctgtg
    32581 aggcaggggc ctataacacc attgatttgg catggattgt gtcgcgtaaa aagccgctag
    32641 cctcgcgccc ggttcggttg cgtttgccgt tcaacaacgg gcaggagacg aatgaactgg
    32701 aactgatgaa cgcaacattt gatgagaaat cgcgtgaact ggtgacctta gcgaaagggc
    32761 gcggattaag cgattgtggc attcaggcgc gctggcgctt tgacggtcag cgttttcgcc
    32821 tggtgcgtta cgccgcagaa cccacctgcg ataactggca tgggccagac gcctggccca
    32881 cgttgtggat cacccgttaa acaaaatggc ggggacatgt aggccggata agacgctgta
    32941 ggccggataa gacgcgtcag cgtcgcatcc ggcaatcagc atccggcgat cactatcagg
    33001 cacctttcac tcccagcacc ttatgcgctt ttgccacaat attctcggcg gtaaagccaa
    33061 agaacgggaa cagcttatcc gccggagcag attccccata acccgtcatc ccgacaattg
    33121 cccctttcag accaacatac ttgtaccagt aatcggcaat acctgcttcc accgccacgc
    33181 gagccgcaac gttagaaggc aacaccgact cccgatattc ctcatcctgg gcgtcgaaaa
    33241 tatcggtcga gggcagggaa accacgcgaa cattgcgacc ttctcctgct aatttctccg
    33301 cagcttgcag ggtgatttcc atctctgaac cggtggcaat cagaataata tctggcttac
    33361 cgccgctgtc tttcagcaca taaccgccac gagtaatctc tttaacctga tccggcgtac
    33421 gttccacctg ggccagattc tgccttgaga ggatcagtgc cgtcggtccg ttgtggcgct
    33481 caaccgccag cttccagccc accgccgctt ccacctgatc gcacggtcgc caggtgctga
    33541 aatttggcgt taagcgcagg ctggccagtt gctcaacagc ctggtgcgtc ggaccatctt
    33601 cgcccaggcc aattgagtcg tgggtataaa ccataatctg ccgcgctttc atcagtgccg
    33661 ccatccgcgc ggcgttacgg gcgtattcaa caaacatcag gaacgtcgcg gtatacggca
    33721 caaagccgcc gtggtgcgcg atgccgttgg cgatagcggt catgccaaat tcacgcaccc
    33781 cgtagtgaat gtagttgccc gccggatctt ccttcagcga aacagaacct ttccagatgg
    33841 tcaggttgct gggagccaga tccgccgaac cgccgagcag ctccggtagc aaggggccat
    33901 aagcgttaag cgtattttgc gaagccttac gggtggcgat tttcgccgga ttcgcctgca
    33961 actcattgat atacttctga gtcgttttct cccagtcctt cggtaaacca ccgctcatcc
    34021 gacgggtaaa ctcttctgcc agttgcggat gagccttttt ataggcggca aacttctcat
    34081 tccacctctg ctgcgctttt tcgccttttt cgcgggcatc ccaggcgtga taaatatctt
    34141 tagggatctc aaatggcgga tggtgccagc ccagtttttg ccgtgccagc gccacttctt
    34201 cttcccccag cggtgcgccg tgcgcctctt ccttacctgc tttattcggc gatccaaagc
    34261 caatcaccgt acgacagata atcagcgacg gcttatcttt cacgctttgc gcttcaagga
    34321 tcgcttcctt caccgcctgc ggatcgtgac cgtcgatttc atggatcaca tgccagtgat
    34381 aggcttcaaa acgttttgcc gtatcgtcgg taaaccagcc ttctgtttca ccgtcgatgg
    34441 aaataccgtt gtgatcgtaa aaaccaatca gcttgcccag tcccagcgtg cctgccagcg
    34501 aacagacttc gtgggaaata ccttccatca ggcagccgtc gcccataaac acataggtga
    34561 agtgatcgac gatctcatgg tctggctggt taaactgcgc cgccagtgta cgctctgcta
    34621 tcgccagccc gacggcgttc gccaaacctt gtccaagtgg accggtggtg gtttcaacgc
    34681 ctggcgtata accaatctcc gggtggcctg gggttttcga atgcagttga cgaaagttct
    34741 tcagctcttc cagcggcagg tcgtaaccgg tcagatgcag caaactgtag agcagcatcg
    34801 acgcgtgacc gttggaaaga ataaagcggt cgcgatcata ccaggttggg tcggtagggt
    34861 tatgtttaag aaaatcgttc cacagcactt cggcaatatc agccatgccc atcggcgcgc
    34921 cgggatgacc agaattggct ttttgtaccg catccatact gagtgcgcga atcgcattgg
    34981 caaggtcttt tcgggacata taacactccg tggctggttt atagtttggc ggcaagaaga
    35041 tcttccagtt tgcgttgatc aacggcgaac agacgaatgc cttcagacag tttttctacc
    35101 gccatcgcat actgattgtg ctcccaacgg aactccgctt cgctcatggg agctgggcgt
    35161 gggaacgtct gagaaggtgg gattaattta cgtaccactg gcgaaacttt ttcctgcagc
    35221 tccttcagca aattcggtgc gatagtcagt cgatcgcagc cggttaaggc gaggatttgt
    35281 tcggtgcgac ggaagctcgc gcccatcaca atggtttcat agtggtgttg cttatagtag
    35341 tcgtagatat tgcgcaccga tttaacgccc ggatcttctt ccaccacata cgggtccatc
    35401 ggcttgcgtg cctgatacca gtcataaata cgcccgacaa acggcgaaat cagaaaaacg
    35461 cctgcttccg cacaggcccg tgcctgtgca aaagaaaaca gcagcgtcag gttgcagtta
    35521 ataccttctt tttccagctc ttctgccgcg cgaattcctt cccaggtcga agccagcttg
    35581 atcagaatgc gtgatttctc aacgccttgt tgctgataca agtccaccag atggcgcgcc
    35641 ttctcaatac tcttttcttt atcaaaagag aggcgtgcat caacttctgt tgacacgcga
    35701 ccgggtacga ttttgaggat ttcagcaccg aaattgaccg ccagtttgtc acacgctgcg
    35761 accacctgtt gttcctgggt cttgccattt tttttacccc aggcgatagc atcgtctatt
    35821 aaatgctcat attgtgataa tccggcagcc ttgagtaaca gcgaaggatt ggtggtggca
    35881 tcctggggat gataatggcg aatggactca atatcgccgc tgtctgccac gacagtggtg
    35941 aactgtttga tgccgtctaa ctcgttcata ggaaatactc cttgaaaagt aaagtgttag
    36001 atgagtgcgt taattcacac ttctgagaaa tttcgctaaa cgcatcaaaa aagcatagca
    36061 gacaggcatg gtattgctgg attaagcagg taacatcagt gttataggat tattaccaaa
    36121 acattatatt aatggtgaga gtttggactt gctcaaagtc tgtagactcc ggcaaggtaa
    36181 taatgtgcgc cacgttgtgg gcagggggtg ttgcccacac actttatttg tgaacgttac
    36241 gtgaaaggaa caaccaaatg gatgaccagt taaaacaaag tgcacttgat ttccatgaat
    36301 ttccagttcc agggaaaatc caggtttctc caaccaagcc tctggcaacg cagcgcgatc
    36361 tggcgctggc ctactcaccc ggcgttgccg caccttgtct tgaaatcgaa aaagacccgt
    36421 taaaagccta caaatatacc gcccgcggta acctggtggc ggtgatctct aacggtacgg
    36481 cggtgctggg gttaggcaac attggcgcgc tggctggcaa accggtgatg gaaggcaagg
    36541 gcgttctgtt taagaaattc gccgggattg atgtatttga cattgaagtt gatgaactcg
    36601 acccggacaa atttattgaa gttgtcgctg cgctcgaacc gaccttcggc ggaatcaacc
    36661 tcgaagacat taaagcgcca gaatgtttct atattgaaca gaaactgcgc gagcggatga
    36721 atattccggt attccacgac gatcagcacg gcacggcaat catcagcact gccgccatcc
    36781 tcaacggctt gcgcgtggtg gagaaaaaca tctccgacgt gcggatggtg gtttccggcg
    36841 cgggtgccgc agcaatcgcc tgtatgaatc tgctggtagc gctgggtctg caaaaacata
    36901 acatcgtggt ttgcgattca aaaggcgtta tctatcaagg ccgtgagcca aacatggcgg
    36961 aaaccaaagc cgcatatgcg gtggtggatg acggcaaacg taccctcgat gatgtgattg
    37021 aaggtgcgga tattttcctg ggctgttccg gcccgaaagt gctgacccag gaaatggtga
    37081 agaaaatggc tcgggcacca atgattctgg cgctggcgaa tccggaaccg gaaattctgc
    37141 cgccgctggc gaaagaagtg cgtccggatg ccatcatctg caccggtcgt tctgactatc
    37201 caaaccaggt caacaacgtc ctttgcttcc cgttcatctt ccgtggcgcg ctggacgttg
    37261 gcgcaaccgc catcaacgaa gagatgaaac tggcggcggt acgcgcgatt gcagaactgg
    37321 cccatgcgga acagagcgaa gtggtggctt cagcgtatgg cgatcaggat ctgagctttg
    37381 gtccggaata catcattcca aaaccgtttg atccgcgctt gatcgttaag atcgctcctg
    37441 cggtcgcgaa agcggcgatg gagtcgggcg tggcgacgcg tccgattgct gatttcgacg
    37501 tctacatcga caagctgact gagttcgttt acaaaaccaa cctgtttatg aagccgattt
    37561 tctcccaggc tcgcaaagcg ccgaagcgcg tagttctgcc ggaaggggaa gaggcgcgcg
    37621 ttctgcatgc cactcaggaa ctggtaacgc tgggactggc gaaaccgatc cttatcggtc
    37681 gtccgaacgt gatcgaaatg cgcattcaga aactgggctt gcagatcaaa gcgggcgttg
    37741 attttgagat cgtcaataac gaatccgatc cgcgctttaa agagtactgg accgaatact
    37801 tccagatcat gaagcgtcgc ggcgtcactc aggaacaggc gcagcgggcg ctgatcagta
    37861 acccgacagt gatcggcgcg atcatggttc agcgtggcga agccgatgca atgatttgcg
    37921 gtacggtggg tgattatcat gaacatttta gcgtggtgaa aaatgtcttt ggttatcgcg
    37981 atggcgttca caccgcaggt gcaatgaacg cgctgctgct gccgagtggt aacaccttta
    38041 ttgccgatac ctacgtcaat gacgaaccag acgcagaaga gctggcggag atcaccttga
    38101 tggcggcaga aactgtccgt cgtttcggta ttgaaccgcg cgttgctctg ttgtcgcact
    38161 ccaactttgg ttcttctgac tgcccgtcgt cgagcaaaat gcgtcaggcg ctggaactgg
    38221 tcagggaacg tgcgccagaa ctgatgattg atggtgaaat gcacggcgat gcggcgctgg
    38281 tggaagcgat tcgcaacgac cgtatgccgg acagcccttt gaaaggttct gccaatattc
    38341 tggtgatgcc gaacatggaa gctgcccgca ttagttacaa cttactgcgc gtttccagct
    38401 cggaaggtgt gactgtcggt ccggtgctga tgggcgtggc gaaaccggtt cacgtgttaa
    38461 cgccgatcgc atcggtgcgt cgtatcgtca acatggtggc gctggccgtg gttgaagcgc
    38521 aaacccaacc gctgtaattc ttttttaact ctcacgctta ccctgaatat tcagggtaag
    38581 cggtttggct gcaatatatt agtaaagctt a
//
LOCUS       DAZPJR010000020        38337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPJR010000020 DAZPJR010000000
VERSION     DAZPJR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.021"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHVB7_000978"
     CDS             59..1423
                     /locus_tag="ACHVB7_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1913037.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHVB7_000979"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHVB7_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR1913038.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHVB7_000980"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHVB7_000980"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR1913039.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHVB7_000981"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHVB7_000981"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR1913040.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHVB7_000982"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHVB7_000982"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR1913041.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHVB7_000983"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHVB7_000983"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR1913042.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHVB7_000984"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHVB7_000984"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR1913043.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHVB7_000985"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHVB7_000985"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR1913044.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHVB7_000986"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHVB7_000986"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR1913045.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHVB7_000987"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHVB7_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR1913046.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHVB7_000988"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHVB7_000988"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR1913047.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHVB7_000989"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHVB7_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR1913048.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHVB7_000990"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHVB7_000990"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR1913049.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHVB7_000991"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHVB7_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR1913050.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHVB7_000992"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHVB7_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR1913051.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHVB7_000993"
     CDS             13749..14288
                     /locus_tag="ACHVB7_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1913052.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHVB7_000994"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHVB7_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR1913053.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHVB7_000995"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHVB7_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR1913054.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHVB7_000996"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHVB7_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR1913055.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHVB7_000997"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHVB7_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR1913056.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHVB7_000998"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHVB7_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR1913057.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHVB7_000999"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHVB7_000999"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR1913058.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHVB7_001000"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHVB7_001000"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR1913059.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHVB7_001001"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHVB7_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR1913060.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHVB7_001002"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHVB7_001002"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR1913061.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHVB7_001003"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHVB7_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR1913062.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHVB7_001004"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHVB7_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR1913063.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHVB7_001005"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHVB7_001005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR1913064.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHVB7_001006"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHVB7_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR1913065.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHVB7_001007"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHVB7_001007"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR1913066.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHVB7_001008"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHVB7_001008"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR1913067.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="ACHVB7_001009"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="ACHVB7_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR1913068.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHVB7_001010"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHVB7_001010"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR1913069.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHVB7_001011"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHVB7_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR1913070.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="ACHVB7_001012"
     CDS             complement(37465..37920)
                     /locus_tag="ACHVB7_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913071.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="ACHVB7_001013"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="ACHVB7_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9471 a        10314 c         9728 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaaacccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacac ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactac cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cactcccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc ccccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctatcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctagcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcacgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgcctggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacaa cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ctgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccaccag tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaggcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggat gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAZPJR010000021        37768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPJR010000021 DAZPJR010000000
VERSION     DAZPJR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.022"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHVB7_001014"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHVB7_001014"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1913072.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHVB7_001015"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHVB7_001015"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR1913073.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHVB7_001016"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHVB7_001016"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR1913074.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHVB7_001017"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHVB7_001017"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR1913075.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHVB7_001018"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHVB7_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR1913076.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHVB7_001019"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHVB7_001019"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR1913077.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHVB7_001020"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHVB7_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR1913078.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHVB7_001021"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHVB7_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR1913079.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHVB7_001022"
     CDS             6537..6773
                     /locus_tag="ACHVB7_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR1913080.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHVB7_001023"
     CDS             6776..7633
                     /locus_tag="ACHVB7_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1913081.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHVB7_001024"
     CDS             7654..9645
                     /locus_tag="ACHVB7_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR1913082.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHVB7_001025"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHVB7_001025"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR1913083.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHVB7_001026"
     CDS             complement(10248..11144)
                     /locus_tag="ACHVB7_001026"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR1913084.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHVB7_001027"
     CDS             complement(11193..11432)
                     /locus_tag="ACHVB7_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR1913085.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHVB7_001028"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHVB7_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR1913086.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHVB7_001029"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHVB7_001029"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR1913087.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHVB7_001030"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHVB7_001030"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR1913088.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHVB7_001031"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHVB7_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR1913089.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHVB7_001032"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHVB7_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHVB7_001033"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHVB7_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR1913090.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHVB7_001034"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHVB7_001034"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR1913091.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHVB7_001035"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHVB7_001035"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR1913092.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="ACHVB7_001036"
     CDS             complement(21570..22739)
                     /locus_tag="ACHVB7_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1913093.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHVB7_001037"
                     /gene_synonym="ynhF"
     CDS             complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHVB7_001037"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR1913094.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="ACHVB7_001038"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="ACHVB7_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR1913095.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHVB7_001039"
     CDS             complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHVB7_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR1913096.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /gene="punC"
                     /locus_tag="ACHVB7_001040"
     CDS             25360..26571
                     /gene="punC"
                     /locus_tag="ACHVB7_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR1913097.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="ACHVB7_001041"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="ACHVB7_001041"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR1913098.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHVB7_001042"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHVB7_001042"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR1913099.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHVB7_001043"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHVB7_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR1913100.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="ACHVB7_001044"
     CDS             complement(30320..31576)
                     /locus_tag="ACHVB7_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1913101.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="ACHVB7_001045"
     tRNA            31884..31960
                     /locus_tag="ACHVB7_001045"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="ACHVB7_001046"
     tRNA            31965..32041
                     /locus_tag="ACHVB7_001046"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="ACHVB7_001047"
     CDS             32149..32454
                     /locus_tag="ACHVB7_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR1913102.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="ACHVB7_001048"
     CDS             32580..34184
                     /locus_tag="ACHVB7_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR1913103.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHVB7_001049"
     CDS             complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHVB7_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR1913104.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHVB7_001050"
     CDS             complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHVB7_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR1913105.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     VVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="ACHVB7_001051"
     CDS             complement(35794..36462)
                     /locus_tag="ACHVB7_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1913106.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="ACHVB7_001052"
     CDS             complement(36526..37164)
                     /locus_tag="ACHVB7_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR1913107.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..>37768)
                     /locus_tag="ACHVB7_001053"
     CDS             complement(37177..>37768)
                     /locus_tag="ACHVB7_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HFR1913108.1"
                     /translation="GLPLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLR
                     VCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIF
                     TLHRAYTVKLMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYK
                     EMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         9023 a         9930 c         9380 g         9435 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaacca cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccg
//
LOCUS       DAZPJR010000022        37257 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPJR010000022 DAZPJR010000000
VERSION     DAZPJR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.023"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..642)
                     /locus_tag="ACHVB7_001054"
     CDS             complement(<1..642)
                     /locus_tag="ACHVB7_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR1913109.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="ACHVB7_001055"
     CDS             complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="ACHVB7_001055"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR1913110.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="ACHVB7_001056"
     CDS             complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="ACHVB7_001056"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR1913111.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="ACHVB7_001057"
     CDS             complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="ACHVB7_001057"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR1913112.1"
                     /translation="MAKAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="ACHVB7_001058"
     CDS             complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="ACHVB7_001058"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR1913113.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="ACHVB7_001059"
     CDS             complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="ACHVB7_001059"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1913114.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7179..8414
                     /gene="moeA"
                     /locus_tag="ACHVB7_001060"
     CDS             7179..8414
                     /gene="moeA"
                     /locus_tag="ACHVB7_001060"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR1913115.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8414..9163
                     /gene="moeB"
                     /locus_tag="ACHVB7_001061"
     CDS             8414..9163
                     /gene="moeB"
                     /locus_tag="ACHVB7_001061"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR1913116.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="ACHVB7_001062"
     CDS             complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="ACHVB7_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1913117.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10135..11034
                     /locus_tag="ACHVB7_001063"
     CDS             10135..11034
                     /locus_tag="ACHVB7_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR1913118.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11040..13472
                     /locus_tag="ACHVB7_001064"
     CDS             11040..13472
                     /locus_tag="ACHVB7_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR1913119.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13618..14433
                     /gene="ybiV"
                     /locus_tag="ACHVB7_001065"
     CDS             13618..14433
                     /gene="ybiV"
                     /locus_tag="ACHVB7_001065"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR1913120.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14585..15850
                     /locus_tag="ACHVB7_001066"
     CDS             14585..15850
                     /locus_tag="ACHVB7_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR1913121.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15817..16977)
                     /locus_tag="ACHVB7_001067"
     CDS             complement(15817..16977)
                     /locus_tag="ACHVB7_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1913122.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17215..18516
                     /locus_tag="ACHVB7_001068"
     CDS             17215..18516
                     /locus_tag="ACHVB7_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1913123.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18531..20894
                     /locus_tag="ACHVB7_001069"
     CDS             18531..20894
                     /locus_tag="ACHVB7_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR1913124.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21074..22669)
                     /locus_tag="ACHVB7_001070"
     CDS             complement(21074..22669)
                     /locus_tag="ACHVB7_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR1913125.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22888..23808
                     /gene="ldtB"
                     /locus_tag="ACHVB7_001071"
     CDS             22888..23808
                     /gene="ldtB"
                     /locus_tag="ACHVB7_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1913126.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23867..24985)
                     /locus_tag="ACHVB7_001072"
     CDS             complement(23867..24985)
                     /locus_tag="ACHVB7_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR1913127.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="ACHVB7_001073"
     CDS             complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="ACHVB7_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR1913128.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25635..25763
                     /gene="mntS"
                     /locus_tag="ACHVB7_001074"
     CDS             25635..25763
                     /gene="mntS"
                     /locus_tag="ACHVB7_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR1913129.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26035..27618
                     /gene="opgE"
                     /locus_tag="ACHVB7_001075"
     CDS             26035..27618
                     /gene="opgE"
                     /locus_tag="ACHVB7_001075"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR1913130.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="ACHVB7_001076"
     CDS             complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="ACHVB7_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR1913131.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28235..28300)
                     /locus_tag="ACHVB7_001077"
     CDS             complement(28235..28300)
                     /locus_tag="ACHVB7_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913132.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28535..29422
                     /gene="rhtA"
                     /locus_tag="ACHVB7_001078"
     CDS             28535..29422
                     /gene="rhtA"
                     /locus_tag="ACHVB7_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR1913133.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29721..30224
                     /gene="dps"
                     /locus_tag="ACHVB7_001079"
                     /gene_synonym="pexB"
     CDS             29721..30224
                     /gene="dps"
                     /locus_tag="ACHVB7_001079"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR1913134.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30628..31374
                     /gene="glnH"
                     /locus_tag="ACHVB7_001080"
     CDS             30628..31374
                     /gene="glnH"
                     /locus_tag="ACHVB7_001080"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR1913135.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31513..32172
                     /gene="glnP"
                     /locus_tag="ACHVB7_001081"
     CDS             31513..32172
                     /gene="glnP"
                     /locus_tag="ACHVB7_001081"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR1913136.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32169..32891
                     /gene="glnQ"
                     /locus_tag="ACHVB7_001082"
     CDS             32169..32891
                     /gene="glnQ"
                     /locus_tag="ACHVB7_001082"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR1913137.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            33008..35233
                     /gene="ybiO"
                     /locus_tag="ACHVB7_001083"
     CDS             33008..35233
                     /gene="ybiO"
                     /locus_tag="ACHVB7_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR1913138.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35230..36156)
                     /gene="rlmF"
                     /locus_tag="ACHVB7_001084"
     CDS             complement(35230..36156)
                     /gene="rlmF"
                     /locus_tag="ACHVB7_001084"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR1913139.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36432..36692
                     /gene="mcbA"
                     /locus_tag="ACHVB7_001085"
     CDS             36432..36692
                     /gene="mcbA"
                     /locus_tag="ACHVB7_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR1913140.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36957..>37257
                     /locus_tag="ACHVB7_001086"
     CDS             36957..>37257
                     /locus_tag="ACHVB7_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1913141.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9032 a        10058 c         9338 g         8829 t
ORIGIN      
        1 caaatcgtga tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg
       61 atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca
      121 cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg
      181 tccatttact tgttcctgat gataaacaac gattttatca ttcgccctcc gcgaaaagag
      241 ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat
      301 tttcagaatc acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt
      361 taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag
      421 ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat
      481 gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt
      541 tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg
      601 tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
      661 atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt
      721 tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc
      781 atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc
      841 gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc cccactctgg
      901 tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt
      961 gccaatgcgc atggtgaaaa acaccacgat agacgagacg gtcccaggca agatatgacg
     1021 caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg
     1081 tttcaacacc agcgtgttgc cgcgcaccag tcgggcaaac gcggggatgg aaaaaatggc
     1141 gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag
     1201 taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca
     1261 gccttcataa tatccagcca acaagcccag caacgtcccg atcgccgcac cgataaacac
     1321 ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc
     1381 gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc
     1441 ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac
     1501 aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg
     1561 ccagaattca tgccacggtg tacgcacctg gtcaggtttg accagtggca tggcgtttaa
     1621 caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta
     1681 atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc
     1741 agaatttccg cctgaatcac cgggtaatcg cgcatttcta cggagtcaac gagtaagcgt
     1801 ccaagtccgg gccagttgaa aactttctcc accacgatag aaccaccgag caaaaagcca
     1861 aactgtaagc ccatcatggt cactaccggg atcatcgcgt tacgtagccc gtgtttgagg
     1921 acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa
     1981 acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg ctgccactgc cgcgccgagc
     2041 gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacggtagg cagccagccc
     2101 agttcaacgg agaatacctg aattaaaagc atccccagcg caaatgccgg aaacgagatc
     2161 cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca gacggcggcg
     2221 ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccac
     2281 agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac
     2341 aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc
     2401 tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgagcccg
     2461 gcaatcaatc gcgccggatc gccgggcagc atatgcacaa ataaaaacac cagcaccgag
     2521 acgataaaca gcgtcggaat caaccccagt aagcgtttga taacgtaatt aagcattcca
     2581 ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccggtg tctggcatga
     2641 tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga
     2701 tccacggcga ctcttgccag atgatatcct gcgccgcctt atacaagcgg gtcttttccg
     2761 ccggatcatt agttttcagt gcctgagcca ggaagtcgtc cacctgttta ttgctgtaaa
     2821 atgcggtatt aaacagcgtc ggtggccagt tttgcgaggc gaacagtggc gatagcgccc
     2881 agtccgcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccgctctctt
     2941 tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg gtcacctggg
     3001 ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc
     3061 tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca
     3121 gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga
     3181 tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca actttcacca
     3241 gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct
     3301 tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc atcaactcga
     3361 tatttttgtt tttctccagc agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg
     3421 cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta
     3481 tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaattttttc accttcacaa
     3541 aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa
     3601 tctccttgcc atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc ggatgggcaa
     3661 gaatattaat aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga
     3721 tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat
     3781 cgctggcccg gtccagattc gcttttaccg ccactgcgtt gaaatcggtg ccatcctgga
     3841 atttaatgcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac
     3901 tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa
     3961 acgatttcgc tacggcctga gataacgtat cattcgcgtc atacggatcg agcgtggtga
     4021 aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg
     4081 ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agcttttgcc attttattct
     4141 ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc
     4201 cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg
     4261 ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc
     4321 ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcgc
     4381 cgtggaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc
     4441 tctaccacgg ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga
     4501 tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga aacggcttcg
     4561 tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc
     4621 tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc
     4681 tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc gtgtacacgc
     4741 agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc
     4801 tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccgggtga caaggtatcg
     4861 attctctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc
     4921 cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc
     4981 cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa
     5041 ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc gtaaaacagg ttcgccatcc
     5101 accaccgttt tctgctcgat ggggggggcc tgtttcgctg gatgttcaag cgatatcaac
     5161 gggaaacgtc ggggataatc caaccctttc atcgcaccaa gttgcggaac agcagctaac
     5221 agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc
     5281 accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac cacgcccata
     5341 tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc
     5401 aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc
     5461 gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt
     5521 tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa tgcgtacctg atccagcatc
     5581 cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg
     5641 gcaatctgtt cacccacagt aaataccggg ttcagcgatg tcatcggctc ctgaaatatc
     5701 atcgccatat ccgcaccgcg aacatggcgc atttgtgcag cgctctgctc gctaagttca
     5761 atcacttcgc gactgcgccg ctgcaacagc attttatcgc actgtactaa accgcccgcc
     5821 tgttccaaca ggcgcatcaa tgccaacgca gtcactgact taccggagcc ggattcgcca
     5881 acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt
     5941 ttctgctggt cctgcataaa ggcaatattc agattttcaa ccgccagcac attaccggca
     6001 tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg
     6061 cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac
     6121 attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc
     6181 cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc
     6241 gatgtcatat gccgccagcg cgcggatgaa gacttcgccc gtgccgatac aagaaaccgc
     6301 cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc caactcgtcc
     6361 gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccaaattgc cgtctaaatc
     6421 caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg
     6481 gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt tgttcataac gcaaaggcgt
     6541 ggagaaaatc tccggcgaga cgcgctccat gccacgagca aacgcaaaat tttctgcccc
     6601 ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg caagaaccgg
     6661 attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac
     6721 acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa
     6781 cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc
     6841 cgcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcaa tgtagcgtaa
     6901 ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat
     6961 gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat
     7021 cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta
     7081 aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tgtggcgttt ttctgacata
     7141 ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga
     7201 tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt caccccactg accgcccagg
     7261 aaacgctgcc actggtacag tgttttggtc gtattctggc gagcgatgtc gtttcgccgc
     7321 ttgatgttcc ggggtttgat aactccgcaa tggacggcta cgcggtgcgt ttagccgata
     7381 ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccgtatcatg
     7441 gtgaatggcc tgcgggcacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct
     7501 gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg
     7561 ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg
     7621 ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt gcttcactgg
     7681 ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg
     7741 aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc aaccgtctcg
     7801 ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg
     7861 acgatcccca tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga
     7921 tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt cttgaagagc
     7981 tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta
     8041 aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct
     8101 tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc
     8161 ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt
     8221 tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg accaccggac
     8281 atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc gtgctggaac
     8341 gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt agaaccgttt aacgcgttgt
     8401 tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca accggcaaat
     8461 cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc gtgtgctgat
     8521 cgtcggcctc ggtggactcg gctgcgcagc ttcgctgtat ctggcaagcg ccggtgtcgg
     8581 caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac gccagacact
     8641 gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ccctgacgcg
     8701 gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg cagaacttgc
     8761 agcattgatt gctgaacacg atctggtgct cgactgtacg gataacgttg cggtacgtaa
     8821 tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg
     8881 tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct
     8941 tagccgtttg tttggcgaga acgcattaac ctgcgtagaa gcaggcgtga tggcaccgtt
     9001 gatcggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg
     9061 caaaccgacc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat
     9121 gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaggcct
     9181 gataagacgc gacaagcgtc gcatcaggca tttgctccag acgccagatg cggcgtgaac
     9241 gccttatctg gcctacggtt cgggcacaga tttccaagcg cggcaggcat cggtgcactc
     9301 cgccacgtca tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca
     9361 aacgctccct gccagtcctg ctcaaacttc gccacagcgg cttccaccgc cggatagcta
     9421 atcatctgtt gtgccacatc cagtggcaga gtaattgatt cacatcctgc cagtaagcag
     9481 tccagcgcct gacgcggggt tttgaaactc gctgccagca ctttcgcctg cggcgcatgc
     9541 attttcaata actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc ctgagcatca
     9601 atacgattaa cgtaaggcgc aacatattcc gcacctgcca gcgccgacag caacccttgt
     9661 gctgcgccat ataccgcggt tcccagcgtt ggaatccctt ccgcttttaa catcttaata
     9721 gctgccagcc cctcggcggt caccggaact ttcaccacga tatccgcaat aatagaacgc
     9781 aatttacgtg cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga
     9841 cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca caacatccag cggtttttta
     9901 cccgcggcga taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc
     9961 gccttcaccg caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg
    10021 tactttttgt ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca
    10081 atctaacttt cattcgaaag tattttaatg tttgaacgaa acagagaggc gactatgatt
    10141 ttcaatattc agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt
    10201 aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgcacgcag
    10261 gatctgctgt atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc taaggccgcg
    10321 ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa gttaaccccg
    10381 gagcatctga cggcgttaac cgactgctgt ccgacacagg cattaaccgt gtgtggtgaa
    10441 gtgaaaagcg ttgaggagat catgacgacc gttctgcgcg ataaaccgtt ttacgatcgc
    10501 agcggcggcg gtttaacgct ttcgggtggt gagcccttta tgcagccgga aatggcgatg
    10561 gcgctactgc aagccagcca cgaggcaggc attcatactg cggtagaaac ctgtctgcat
    10621 gtgccgtgga aatatatcgc cccttctctg ccctatatcg atctgtttct tgccgattta
    10681 aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc cagagtgctg
    10741 gataacctga aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt
    10801 cagggcttta atgccgacga aacctctgta aaagctatta ccgattttgc cgccgacgag
    10861 ctgcacgttg gcgaaattca tttcctgccc taccacacgc taggcatcaa caaatatcac
    10921 ttacttaatc tgccctatga cgccccggaa aaaccgcttg atgcgccaga actgctcgac
    10981 tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca
    11041 tgaccacact gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg
    11101 tgcatattgt gaaaccgcca gtctgtaccg agcgcgcgca gcactatacc gagatgtatc
    11161 aacaacatct cgataagccg atcccggtac gtcgcgcgct ggcactggcg catcacctgg
    11221 cgaatcgcac catctggatc aaacacgatg agttgatcat tggcaaccag gcaagcgaag
    11281 ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa gagattgatg
    11341 atctggcaga tcgtcccggt gctggctttg cggtgagcga agagaacaaa cgcgttctgc
    11401 atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga tcgctgctac ggcatgttta
    11461 ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa agcggaaggc aatatgacct
    11521 ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct ggaaaaaggt cttgatggtc
    11581 tgcgcgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtactg gaagatttgc
    11641 acggcgaaca gttcctgaaa gcgattgata tcgtgctggt ggcagtcagc gaacacattg
    11701 aacgttttgc agccctggcg cgtgaaatgg ccgcgaccga aacccgcgaa agccgtcgcg
    11761 atgaactgct ggcgatagca gaaaactgcg atcttatcgc ccaccagccg ccgcagactt
    11821 tctggcaggc gctgcaactg tgttacttca tccaattgat attgcagatc gaatctaacg
    11881 gtcactcggt atcgtttggt cgtatggacc agtatctcta cccgtactat cgccgcgacg
    11941 ttgaactcaa ccagacactg gatcgcgaac acgccatcga attgctgcat agctgctggc
    12001 tgaaactgct ggaagtgaac aagatccgct ccggctcaca ctcaaaagcc tctgcgggaa
    12061 gtcctctgta tcagaacgtc accattggcg gacaaactct ggttgatggt caaccaatgg
    12121 acgcggtgaa tccactctct tacgctatcc tcgaatcctg cggtcgcctg cgttccactc
    12181 agcctaacct cagcgtgcgt taccatgcag gaatgagtaa tgatttcctc gacgcctgcg
    12241 tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa caacgacgaa atcgtgatcc
    12301 cggaatttat taaactcggt attgaaccgc aggacgctta cgactacgca gcgattggtt
    12361 gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg agctttatca
    12421 acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc agcggcaaag
    12481 tgttcctgcc acaagaaaaa gcgttgtcgg caggcaactt taacaatttc gatgaagtaa
    12541 tggacgcgtg ggatacgcaa atccgttatt acacgcgtaa atcaatcgaa atcgaatatg
    12601 tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg ctggtggatg
    12661 actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac tgggtttctg
    12721 gcctgcaggt tggcattgcc aacctcggca acagcctggc ggcagtgaag aaactggtat
    12781 ttgaacaggg cgcgattggt cagcaacagc tggctgccgc actggcggat gacttcgatg
    12841 gcctgactca tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag tacggcaacg
    12901 acgatgatac cgtcgatacg ctgctggctc gcgcttatca gacctatatc gacgaactga
    12961 aacagtacca taacccgcgc tacggtcgtg gtccggttgg cggcaactat tacgcgggta
    13021 cctcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaaca ccggacgggc
    13081 gtaaagccca caccccgctg gcagaaggcg caagcccggc ctccggtact gaccatcttg
    13141 gccctactgc ggtcattggc tcagtgggta aactgcctac ggcagcgatt ctcggcggcg
    13201 tgttgctcaa ccagaaactg aatccggcaa cgctggagaa cgaatctgac aagcagaaac
    13261 tgatgatcct gctgcgtacc ttctttgaag tgcataaagg ctggcatatt cagtacaaca
    13321 tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac cgcgatctgg
    13381 tagtgcgtgt cgcgggctat tccgccttct tcaccgcgct ctctccagac gcacaggacg
    13441 atatcatcgc ccgtactgaa catatgctgt aatctcctcg gcccggcggc gtcgtcgccg
    13501 ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat aatttgtgct
    13561 ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccaggag cacacctatg
    13621 agcgtaaaag ttatcgtcac agacatggac ggtacttttc ttaacgacgc caaaacgtac
    13681 aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat taagttcgtt
    13741 gtcgccagcg gtaatcagta ttaccagctt atttcgttct ttcctgagct aaaggatgag
    13801 atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca actgttccac
    13861 ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa agataagcaa
    13921 ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc ccccgaagca
    13981 tttgtcgcac tgatggcaaa acactaccat cgcctgaaag ctgtaaaaga ttatcaggag
    14041 attgacgacg tactgttcaa gttttcgctc aacctgccag atgaacaaat cccgttagtg
    14101 atcgacaaac tgcacatagc gctcgatggc attatgaaac ccgtcaccag tggttttggc
    14161 tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg gttactgaaa
    14221 cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa cgatgcggag
    14281 atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa cattaaacaa
    14341 atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt gattcaggcg
    14401 gtgctggata acacatcccc ttttaacagc tgacctctct accggagccg tgcgctccgg
    14461 ttttcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc attaacttat
    14521 ttataactta tattgtcgca tgcaaatcag aatggtttcg aatgaaagtt aatgagaggt
    14581 ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag cagctatccg
    14641 tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc cagcagatct ttaatcagct
    14701 aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc
    14761 cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg
    14821 cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc gcgaacaagc
    14881 gctaggctgg gatcagtcga tgctggacta tctggaccgc aaccgctttg acgaggtcta
    14941 caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg agatttaccc
    15001 catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca
    15061 gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga
    15121 tgtgagcgtc atctaccctg accgtatccg ccgccgtccg cccggaacga cctccaaagg
    15181 tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagcg
    15241 cgttttcgcc aacgtcttta atggcaatct ggcgcaatat gatccctggc atgcggcaca
    15301 tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac
    15361 attccaaggc tggacagcgc tctctaatat gctgcctggt caggggctgc tgcacgttgt
    15421 gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga
    15481 ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc
    15541 actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg
    15601 gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt
    15661 gatgtacatt cctgcggcac caatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa
    15721 ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa
    15781 ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggaat
    15841 cgacagctga gtgtcgtaca gcccttcccg ttccacggcg gtgttccctt ttcctgggcg
    15901 aacccgccgc actgactccc gcaccgccaa tcgtccgtgc agcagcagcg agcccaccgg
    15961 agcatccggg cgaatcagcc gctgttgcaa catttgcacc gcttcattgc ctagctcatc
    16021 gcgtggcaca tgcacagccg tcaggggaac atcctcgatt gccgcaaggt taaagccgtc
    16081 gatactcatc accgagacat cctgtggtac gcgcagacca cgacgatgta gcgcatttac
    16141 cgttcccgcc gccataaaat ccccgccgac cagaaacgcc gtcggcaacg tcttgccatt
    16201 ggcggcatcc agccacgctc cgaccttttc ttccgcttct ttagcgctaa aactgctgat
    16261 attcagcaaa tgacggtcat ggtgaaaggc cagattatgc tgacgccatg attctttaat
    16321 ccctgcaaac cgctgctcca tagtgtagcg gcgcaggcac atcacattca tcacctcccg
    16381 gtgacccatt tcaaacagat agcgggcggc gaacgcgcca attaaccgat gatccggcgc
    16441 aatcgccggg aggcgcattc gttcatcacg gcagttgatc agcacacaag gcttagcgag
    16501 atcggcggcc agatcatgga tgtgcggatc gtcaatcccc agcaggatcg ctgcctgcgt
    16561 atctgcttca ttcatccgcg agagaaagag cgcgggatcg ctgtcaaact cctccagcgc
    16621 gcagtaacgc agccgcactt catgcggcga taacgctttg ctgatgctct ggatcacacg
    16681 gtaataaaag atatcagatc gttggtcaaa agcgcgttgc ggcgcgaaaa taatcaggtt
    16741 gttcagcaac atacgacctg ctgccatgcc atccatcacg cccaactcac gcgcgcaggc
    16801 cagcacctgt tcgcgagcgc gtgcactggt attcgctttg ccagccagca cccgcgaaac
    16861 cgtactcggc gatagcccgg tacgtgcggc gatatcggcg attttcagct tcttgtccat
    16921 tttgtgatct gcctctaatt cggaaatgac agaattttca tgagcaaaaa agcccattct
    16981 gtctggcatt aaatccattt cagtaatgta tatcagtcac tcatggaaaa acgtgcacaa
    17041 aacccgcttt ctttcgcttt tagggtctct tactgtgaat tggtagaaca acttccatac
    17101 aaaaataatg gaaaaacgaa caataaagac tttatttatc aaaaagttaa aagtggtgta
    17161 acaactccat tctgacgttg ccgcacaaac cctataccat tctgtggagt gcatatgagt
    17221 cagggtatca ataacgatat gactgccagt aaaccgcgcc atatcgtcaa aaatctgcgt
    17281 tggtggatac tggtcctttt tttgctcggt gttactgtca actacatcac gcgtaactcg
    17341 ctgggtattc tcgcccccga actgaaaagc agcctaggga ttaccaccga acaatattcc
    17401 tggattgtag gtgctttcca gctcgcctat accctattcc aaccgttgtg cggatggctg
    17461 attgacgtca ttggcctcaa actgggcttt atgatctgcg cgggactgtg ggcactggcc
    17521 tgtctgatgc atgcgggggc gggaaactgg ggacatctgg cgctgttgcg cttctttatg
    17581 ggcggtgcgg aagccgcggc aaccccagca aacgccaaaa ccatcggtga atggtttcca
    17641 aaatcagaac gcccgatcgc cgcaggctgg gcaggcgtcg gtttttccat cggtgccatg
    17701 ctggccccac cgattatcta ttttgcccac gcttcattcg gctggcaggg tgcatttatg
    17761 ttcactggca tcctggcgat gctgtgggtg gtgctgtggt gggcgttcta tcacagcccg
    17821 gataagcatc ctaacctcag caaagccgag ctggacttta ttcgccagga taacgaagcg
    17881 cccccggtca agttaccctt tctgaccgca ctgaaaaccg tgggcaaaaa taaacgcttt
    17941 tacggtatcg ctatcccggc ctttatggca gaaccggcgt gggcggtgat gagtttctgg
    18001 gtgcctctat acctgtccaa aacctatggc atggacctga agcagatcgc attattcgcc
    18061 tggttgccgt tcctcgccgc tgatcttggc agtattgcca gcggctatct gaccaaactt
    18121 tacacccgcc tgtttgggct gagtcgtgtg aactccgttg tcgcatcatc ggtcagtggc
    18181 gcattcttga tggtttcgct ggcggtgatg gcctttacca ataacccgta tatcgccatc
    18241 gtccttatct ccatcggcgg cttcggtcac cagatcatct cctgcatgct gagcgcactg
    18301 gtggtggaat ccttcgacaa aggtcagatg gccactgtta acggcatgcg cggttctgcc
    18361 gcgtggatcg caagtttcct gttctccctg attatcgggg ttaccgctga caaaatcggc
    18421 tttaaccccc tgtttatcgc catgggcttc tttgacctga ttggcgcttt cttcctggtc
    18481 acctttattg ctgaacgtcg cgcaaaacgc gcctgagagt ggatgtaaac atgaaaacgc
    18541 tgaaaaactg gaccctgcac aaacagtctg cccaccacgt tgaactgctg gtcgatggcc
    18601 agcatatcct gtgcctgtac gtgctggaag aaaatctgtt ccgcgtgctg ttaaaacgca
    18661 aaggcgagct ggcgctagat cgcacctgga gcattgctcc ggcgcaggat gtgccatggg
    18721 aagggcgcag ccgcgaagat ctcagcggct ttagcctgcc cgcgtggaca gtgatccagc
    18781 acgacgacac cgtggttatc gccaccgagt cactgcgtgc aaccgttcac cagccgctgt
    18841 ggctggagtg gcactatcgt aacaacgaag gcgaatggct gccgttggtg aatgaccgcc
    18901 cgaccagcgc ttatctgctg aatgcccacg gcgatggcgt ggcacactat cagagccgtc
    18961 gtaaagatga acgcttttat ggcctcggag ataaagccgg cgatctgcaa cgcaacggta
    19021 aacgttacga gatgcgtaac ctcgacgcga tgggctacaa cgccgttagt actgacccgc
    19081 tgtacaaaca cattccattc accatcaccc gccgcgatga cattagcttc ggcctgtttt
    19141 atgacaacct cagcagctgc tggctggatc tgggcaacga gatcgacaac taccacaccg
    19201 cctatcgccg ctggcaggcg gaagcgggtg atatcgacta ctacctgttt accggcgaac
    19261 gcgtacttga tatcaccaaa gccttcgtac gcctgaccgg aaaaaccctg ttcggaccga
    19321 aatggagcct gggctacagc ggttcaacta tgcactacac cgatgcgccg gacgcacaaa
    19381 atcagttgat gaactttatc cgcctgtgtg aagaacatgc aattccctgc gattcgttcc
    19441 agctctcttc cggttatacc tcaattaacg gcaaacgcta tgtgttcaac tggaactacg
    19501 acaaagtgcc gcagccgaaa gtgatgagcc aggcatttca caatgctggt ttgcacctgg
    19561 ccgccaatat caagccgtgc ctgttgcagg atcacccacg ctataacgaa gtggcagaaa
    19621 gaggactgtt catccgtgat tcagaaacag atgaaccaga acgttccagc ttctgggatg
    19681 acgaaggttc taacctcgag tttactaatc cgcagaccat ccagtggtgg caggaaggcg
    19741 tgaccacaca gcttctggag atgggtatcg actccacctg gaacgacaac aacgaattcg
    19801 aagtatggga cggtgaagca cgctgtcacg gctttggtca gacgatcgcg atcaaacata
    19861 ttcgcccagt gatgccactg ctgatgatgc gagcctcgat ggaagcccag cagcgtttcg
    19921 ctccggaaaa acgtccgtac ttgatctccc gctccggctg cgcgggaatg cagcgctacg
    19981 tgcagacctg gagtggcgat aaccgcacta gctgggacac tcttcgctac aacacccgca
    20041 tgggtctggg gatgagcctc tccgggctgt ataacctcgg tcacgacgtc ggcggtttct
    20101 ccggtgataa gccggacccg gaactgtttg tacgctgggt acaaaacggc gtgatgcatc
    20161 cgcgctttac gattcactca tggaacgatg accataccgt aaacgaaccg tggatgtatc
    20221 cgggcgtcac tccggcgatc cgcagcgcta ttgagttgcg ctatcgcctg ctgccctact
    20281 tttataccct gatgtggcag gcatacgccg atgatgaacc aatgctacgt ccgactttcc
    20341 ttgaccacga gcacgacgtt cagacttttg aagagtgtga tgacttcctg cttggtcgcg
    20401 atattttggt tgccagcgtc gtagagcaag gcgagcgcca gcgccgtgta tggctgccag
    20461 ataacgaaac tggctggtac gatttctaca acggcgaatg gttcagtggc ggtcagtgga
    20521 tcaccctcga cgcaccgctg gagaaactgc cgctgatggt tcgtgccggg gctggtctac
    20581 cgctcagcaa acgcatcacc tatgtgagcg cagaacaaga cgatacccgc gaactgaaac
    20641 tgttcccgct gaaaggtgtg ggcacaacgt ccggcctgct gtttgaagac gatggcgaaa
    20701 gctggggcta ccaaactggt aatgcgcttt gggtggaatg ggaaatggtt tgcgatgggg
    20761 cgaccatcaa cctgaaagtc aacgcgcgcg gggattatcg tcctgcatgg agtgcactga
    20821 aagtgtcatt accattggaa gaaaaacgca cgctgctggt caatggtgtt gaagggagtg
    20881 aatggatgcg ttaaatcatt accggggccg ggaggcgact gcaccttacc cggtccagcc
    20941 atcgaatcct ttacctcagt atttcacgct gcaggaaggc agcaaacctg taaaccccgg
    21001 gagcttaaat aagtaagtga ccggggtgaa caggtgaagc caatgcatct gccgcatgaa
    21061 agacggcggg gaattaagcg tcgatccctt tactacgcag gtaatcttcg taattaccgc
    21121 taaagtcgat cgcgcgttcc ggggtgattt ccagaatgcg ggtcgccagg gagcttacga
    21181 actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc gccatgttca
    21241 gtgactcgat ggattccata tccaggtggt tggtcggttc gtccatgatc agaatgttcg
    21301 gcttctgcat cattaactta ccaaacagca tccgcccttt ttccccaccg gaaagcactt
    21361 tagctggctt tttgatgtcg tcctggctga acagcaaacg accgagaatg ctgcgtaccg
    21421 cctgctcgtc atcgccttcc tgcttccact gactcatcca ttcgaacacg gtcagatcat
    21481 tttcaaactc atattcgtgg tcctgggcgt agtaaccaat gcgcgcgttt tcagaccatt
    21541 ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcagcagc gttgatttac
    21601 cgacgccgtt ggtacccagt accgcaagtt tttcaccgac ctccagcagc aggttgagat
    21661 ttttaaacag tggaccgtta tcaaaccctt tggtcagacc ttccacttcc agcgcgttac
    21721 ggaacagttt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg gaggctttca
    21781 cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg cgagatttcg
    21841 aggcgttggc gctaaagcgg ctaacgaaag attgcaactc agcaatctgc gctttcttct
    21901 tggcgttatc ggccagcaga cgttcacgcg cctgggtcgc cgccgtcatg tactcatcgt
    21961 agttacccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg gtacagacca
    22021 tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc tcgttcagca
    22081 cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg tcgagcagga
    22141 gaatctccgg atcagcaaac agcgcctgcg ccagaagcac acgcagtttc cagccaggag
    22201 caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca agcaacagtt
    22261 caccggcgcg agcttccgca gagtaaccgt ccatttcgcc gtatttaact tccagatcgg
    22321 ccactttata gccgtcttcc tcactcattt ccggcaaagc atagatgcgg tcacgctcct
    22381 gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca gtgaactctt
    22441 caaaggcaaa ctgatcctga cgcagcttac caatgcgctc gttgggatcg agggaaacgt
    22501 tacccaaagt cggctctaag tcgccgccga ggatcttcat aaaggtggat ttaccactac
    22561 cgttcgcgcc aatcaggccg taacggttgc cgccgccgaa tttgacggaa atgttttcaa
    22621 acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca ggcgtatcct
    22681 gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat cctgctcctc
    22741 gcttaaggtc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca tctgatttca
    22801 ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc aacccaatgg
    22861 cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt attcgcagcg
    22921 gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc tctgccaacc
    22981 gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg taacactcag
    23041 ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat ggaagcgaac
    23101 ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc gcagcagctg
    23161 atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat gcgtctgtat
    23221 tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg tcagttaggc
    23281 aaagatacgc ctatcaactg gaccaccaaa gttgagcgta agaaagcagg cccgacctgg
    23341 acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct tccggctgtc
    23401 gttccggcag gtccggataa cccgatgggg ctgtatgcac tctacatcgg tcgcctgtac
    23461 gctatccatg gcaccaacgc caacttcggt atcggcctgc gtgtaagtca tggttgtgtg
    23521 cgtctgcgta acgaagacat caaattcctg ttcgagaaag taccggtcgg tacccgcgta
    23581 cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg ttatattgaa
    23641 gtccataacc cgctgtctac caccgaagcc cagtttgaag gtcaggaaat tgtgccaatt
    23701 accctgacga agagcgtgca aacagtgacc ggtcagccag atgttgacca ggttgttctt
    23761 gatgaagcga ttaaaaaccg ctccgggatg ccggttcgtc tgaattaatc ttcaaaactt
    23821 aaagcaaaag gcggactcat aatccgcctt ttttatttgc cagaccctag ttggccggga
    23881 gtataactaa caaaacatat cccaccaacg ccgcccacaa cagcatcggt attgaataga
    23941 gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca
    24001 gcgatccggg taataaccca aagccaccta cgtttaccgc ccataccagt aataaagacg
    24061 gcggcacata gttcagcaac aatatggtac tcggcacgtt actgatcacc tgcgataaac
    24121 cgattgccgt taaccataac ccgggttcag atagatgact cacgttaccc aacacgcctt
    24181 gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca
    24241 gcagcgtcca gtccacactg agcaccacgc gacgtgccag cagcgcaaag cctgccgcca
    24301 caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca
    24361 gccccagaca actccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt
    24421 attgcagcgc ctttccaggg aaacaacacc agcacaggag caggagcgtc agcatcattg
    24481 cgccagccag cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc
    24541 agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca
    24601 gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag
    24661 tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg
    24721 ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc
    24781 gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc
    24841 ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca
    24901 cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca
    24961 gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaagc
    25021 gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc
    25081 cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc
    25141 ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat
    25201 cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg aaactcccag
    25261 acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag
    25321 ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc
    25381 aacgtgctct tccacgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg
    25441 acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca
    25501 cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctacataat
    25561 atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca
    25621 agtcaggagg tcttatgaat gagttcaaga agtgtatgcg cgtgtttagt cattctccct
    25681 ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc
    25741 aagataaacc ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg
    25801 caggcataac gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc
    25861 gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc
    25921 cctacgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag
    25981 gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat
    26041 ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc
    26101 tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac
    26161 accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa
    26221 gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac
    26281 ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc
    26341 gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc
    26401 ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac
    26461 agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc
    26521 tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat
    26581 gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga
    26641 atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg
    26701 atcggcgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc
    26761 ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc
    26821 acgcagaaat cacttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag
    26881 gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag
    26941 ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg
    27001 tacttcctga aagaaggtaa tttcgaagca gataaaaaca cgaaagacga agcgttactg
    27061 gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat
    27121 ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga aacctttgtg
    27181 caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc
    27241 aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta cttttctgac
    27301 cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa
    27361 tatcagcaaa atttccaggt gccttttatg gtcatttcca gcgacgataa agcgcatcgg
    27421 gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga
    27481 attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg
    27541 atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt
    27601 ttcgacccga aaccttaaaa caaaaaatcc gccccgagag gcggattttt tatatcacca
    27661 aagtgattag aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat
    27721 acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc
    27781 agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt
    27841 ctggaattta ccataaccca cccccactac accgtagatg cttgcccagt cgttaatgcg
    27901 gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc
    27961 agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc tgttgtcttc
    28021 ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct
    28081 ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc
    28141 cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat
    28201 tcaaataagt cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt
    28261 gatgtctaac gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat
    28321 ttaaatcact ttttcagaga actgcgtaag tattacgcat gttttccctg tcattcatcc
    28381 agattattcc taatcaccag actaatgatt ccatcaatcc tggcgcattt tagtcaaaac
    28441 gggggaaaat tttttcaaca aatgctcaac cagcattggg tatatccagt acactccacg
    28501 ctttacttaa gtctagatat ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc
    28561 ggtctggtta ccaatagtca tattgctcgt tgccatggcg tctattcagg gtggagcctc
    28621 gttagctaag tcactttttc ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc
    28681 attaggaacg ctgatcctca tcgcgttctt taagccatgg cgactgcgct ttgccaaaga
    28741 gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt gggatgaatt atctttttta
    28801 tctttctatt cagacagtac cgctgggtat tgcggtggcg ctggagttca ccggaccact
    28861 ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt
    28921 tcttggtctg tggttcctgc taccgctggg gcaagacgtt tcccatgtcg atttaaccgg
    28981 ctgtgcgctg gcactggggg ccggggcttg ttgggctatt tacattttaa gtgggcaacg
    29041 cgcaggagcg gaacatggcc ctgcgacggt ggcaattggt tcgttgattg cagcgttaat
    29101 tttcgtgcca attggagcgc ttcaggctgg tgaagcactc tggcactggt cggttattcc
    29161 attgggtctg gctgtcgcta ttctctcgac cgctctgcct tattcgctgg aaatgattgc
    29221 cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc
    29281 tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc tgctggcgct
    29341 cggcgctatc atcgccgctt caatggggtc tacgctgaca gtacgcaaag agagcaaaat
    29401 aaaagaatta gacattaatt aaatttacat ttctgcatgg ttatgcataa ccatgcagaa
    29461 tttctcgcta cttttcctct acaccgtctt tatatatcga attatgcaaa agcatattta
    29521 ttccgaaaat tcctggcgag cagataaata agaattgttc ttatcaatat atctaactca
    29581 ttgaatcttt attagttttg tttttcacgc ttgttaccac tattagtgtg ataggaacag
    29641 ccagaatagc ggaacacata gccggtgcta tacttagtct cgttaattac tgggacataa
    29701 catcaagagg atatgaaatt atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc
    29761 tgctttatac ccgcaacgat gtctccgaca gcgagaaaaa agcaacagta gagttgctga
    29821 atcgccaggt tatccagttt attgatcttt ctttgattac caaacaagcg cactggaaca
    29881 tgcgcggcgc taacttcatt gccgtacatg aaatgctgga tggcttccgc accgcactga
    29941 tcgatcatct ggataccatg gcagaacgtg cagtgcagct gggcggtgta gctctgggga
    30001 ccactcaagt tatcaacagc aaaaccccgc tgaaaagtta cccgctggac atccacaacg
    30061 ttcaggatca cctgaaagaa ctggctgacc gttacgcaat cgtcgctaat gacgtacgca
    30121 aagcgattgg cgaagcgaaa gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg
    30181 acctggataa attcctgtgg tttatcgagt ctaacatcga ataaatccat cgctgatggt
    30241 gcagaacttt agtacccgat aaaagcggct tcctgacagg aggccgtttt gttttgcagc
    30301 ccacctcaac gcacttattt agtgcatcca tctgctatct ccagctgatt aagtaaattt
    30361 tttgtatcca catcatcaca caatcgttac ataaagattg ttttttcatc aggttttacg
    30421 ctaaataatc actgtgttga gtgcacaatt ttagcgcacc agattggtgc cccagaatgg
    30481 tgcatcttca gggtattgcc ctataaatcg tgcatcacgt ttttgccgca tctcgaaaaa
    30541 tcaaggagtt gcaaaactgg cacgattttt tcatatatgt gaatgtcacg caggggatcg
    30601 tcccgtggat agaaaaaagg aaatgctatg aagtctgtat taaaagtttc actggctgca
    30661 ctgaccctgg cttttgcggt ttcttctcat gccgcggata aaaaattagt tgtcgcgacg
    30721 gataccgcct tcgttccgtt tgaatttaaa cagggcgata aatatgtggg ctttgacgtt
    30781 gatctgtggg ctgccatcgc taaagagctg aagctggatt acgaactgaa gccgatggat
    30841 ttcagtggga tcattccggc actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt
    30901 accatcaccg acgagcgtaa aaaagcgatc gatttctctg acggctacta caaaagcggc
    30961 ctgttagtga tggtgaaagc taacaataac gatgtgaaaa gcgtgaaaga tctcgacggg
    31021 aaagtggttg ctgttaagag cggtactggc tccgttgatt acgcgaaagc aaacatcaaa
    31081 actaaagatc tgcgtcagtt cccgaacatc gataacgcct atatggaact gggcaccaac
    31141 cgcgcagacg ccgttctgca cgatacgcca aacattctgt acttcatcaa aaccgccggt
    31201 aacggtcagt tcaaagcggt aggtgactct ctggaagcgc agcaatacgg tattgcgttc
    31261 ccgaaaggta gcgacgagct gcgtgacaaa gtcaacggcg cgttgaaaac cctgcgcgag
    31321 aacggaactt acaacgaaat ctacaaaaaa tggttcggta ctgaaccgaa ataataacgc
    31381 tacacctgta aagcgcactg gcagttccct ctcccctctg gggagaggat tagggtgagg
    31441 ggcgcaaacc cgctccgggg ccattaatta ccctgaattt gattatttac accacggtaa
    31501 caggaacaac atatgcagtt tgactggagt gccatctggc ctgccattcc gcttctgatt
    31561 gaaggtgcca aaatgaccct gtggatttcg gtcctcggtc tggctggcgg tctggtaatc
    31621 ggattgctgg caggttttgc acgcaccttc ggtggttgga tagccaacca cgtcgcgctg
    31681 gtctttattg aggtgatccg cggcacacct atcgtcgtcc aggtgatgtt tatttatttc
    31741 gccctgccga tggcgtttaa cgacttacgc atcgacccat ttactgcggc ggtggtcacc
    31801 atcatgatca actccggcgc gtacattgcg gaaatcacgc gtggtgcggt gctgtctatt
    31861 cacaaaggtt ttcgtgaagc aggactggcg ctcggtcttt cacgttggga aaccattcgc
    31921 tacgtcattt taccgctggc actgcgtcgt atgctgccgc cgctgggtaa ccagtggatc
    31981 atcagcatta aagacacctc gctgtttatt gtgatcggcg tggcggaact gacccgtcag
    32041 gggcaagaaa ttattgccgg taacttccgc gcccttgaga tctggagcgc cgtggcggtg
    32101 ttctatctga ttattaccct ggtgctaagc tttattttgc gtcgtctgga aagaaggatg
    32161 aaaatcctgt gattgaattt aaaaacgtct ccaagcactt tggcccaacc caggtgcttc
    32221 acaatatcga tttgaacatt gcccagggcg aagtcgtggt gattatcggg ccgtccggtt
    32281 ccggtaaatc gaccctgctg cgctgcatca acaaactgga agaaatcacc tccggcgatc
    32341 tgattgtcga tggcctgaag gttaacgatc cgaaagttga cgagcgcctg attcgccagg
    32401 aagcaggtat ggtgttccag cagttttacc tcttcccgca tctgacggcg ctggaaaacg
    32461 tcatgtttgg tccgctacgc gtgcgtggcg cgaacaaaga agaggcggaa aaactggcgc
    32521 gtgagctgct ggcgaaagtc ggtctggcag aacgtgcaca tcactaccct tccgaacttt
    32581 ctggtggtca acagcagcgt gtggcgattg cccgcgcgct ggcggtgaag ccgaaaatga
    32641 tgctgtttga tgaaccgact tccgctcttg acccggaact gcgccatgaa gtgctgaagg
    32701 ttatgcagga tctggctgaa gaagggatga cgatggtgat cgtgacccac gaaatcggtt
    32761 ttgccgagaa agtagcttcg cggctgatct ttatcgacaa aggccggatt gcggaagatg
    32821 gcgatccgca ggtattgatc aagaacccgc cgagccagcg cttgcaggaa tttttgcagc
    32881 acgtctctta acttctctgc ctccctttca cccgaaaggg aggcacacca gattcctctc
    32941 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
    33001 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
    33061 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
    33121 ggaaccggat atcgaacaaa aaaaagcggc ctatggcgca ctggcggatg tgctggataa
    33181 cgacacctcg cgtaaagaat tgatcgacca gttgcgtact gtcgccgcga cgccccctgc
    33241 ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg tgctgcaaaa
    33301 ggtcaccgaa gtcagccgcc attatggtga agccctttcc gcccgcttcg ggcaacttta
    33361 tcgcaatatc accggctccc cgcataagcc atttaatcca caaaccttca gcaatgcgct
    33421 gagccatttt tcaatgttag cggtattagt gtttggtttt tactggctga ttcgcctgtg
    33481 cgcactgccg ctgtatcgca aaatgggtca gtgggcgagg caaaaaaatc gtgagcgcag
    33541 caactggttg cagcttccgg cgatgattat cggggcgttt attatcgacc tgctgttact
    33601 ggccctgaca ttatttgtcg gccaggtatt aagcgacaac ctgaatgcag gcagtcgcac
    33661 catcgctttc caacaaagtt tgtttctcaa cgcctttgcc ctgattgaat ttttcaaagc
    33721 cgtactacgc ctgatttttt gcccaaacgt ggcggagctg cgcccgttca cgattcagga
    33781 cgagagcgcc cgttactgga gtcgtcgcct gagctggtta agcagtttga taggttatgg
    33841 cctgattgtg gccgtgccga ttatctctaa tcaggtgaat gtacagatag gtgcgctggc
    33901 gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg tacctgatct ttcgtaataa
    33961 aaaagagatt acccagcatt tgctcaactt tgcggagcat tcgctggcct ttttcagcct
    34021 gtttatccgc gcctttgcgc tggtgtggca ctggctggca agcgcctatt ttatcgtgct
    34081 gtttttcttt tcgttgttcg atccgggcaa cagcctgaaa tttatgatgg gtgcaacggt
    34141 gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc ggtatgtttt cccgctggct
    34201 ggcaaaaacc atcactctct cgccacatac tcagcgtaac tatccagagt tgcaaaaacg
    34261 gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt attctgaccg tctgcgtggc
    34321 ggtaatgttg ctgttgagcg catggggatt gttcgatttc tggaactggc tgcaaaacgg
    34381 cgcggggcag aaaaccgtag atatcctgat ccgtatcgca cttattcttt ttttctcggc
    34441 ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg atattcatgg
    34501 ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg ctgttccgta acgcgctggc
    34561 ggtgattatc agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg tcaatatcgc
    34621 gccattgctg gcaggtgccg gggcattagg tctggctatc tcgtttggtt cgcaaacgct
    34681 ggtgaaagat atcattaccg gggtgtttat tcagtttgaa aacggcatga acactggaga
    34741 tttggtgact atcgggccgt tgaccggcac agtggaacgg atgtcgattc gctccgtggg
    34801 cgtgcgccag gataccgggg cgtatcacat cattccgtgg tcttcgataa ccacctttgc
    34861 taacttcgtc cgcggtattg gttcggtagt ggcaaactac gatgttgatc gccatgaaga
    34921 tgctgataaa gccaatcagg cactgaaaga tgcggtagcg gaattaatgg aaaacgaaga
    34981 aattcgcggg ctgattattg gtgaaccgaa ttttgccggg attgtcggct taagcaatac
    35041 cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc aaacagtgga cggtacgctt
    35101 tgccctcgac agccaggtga aaaaacattt cgacctggcg ggcgttcgcg cgccagtgca
    35161 gacttatcag gtgctgccta ctccgggcgc gaccccggct gaaccgttac cgccggggga
    35221 gccaacgctt taacgctggc gattgacaaa acggcggcgc tgctcgtcgt tcataaaggt
    35281 ccaggcaata aagcgactct gcttttgccc ctgggccatc tcttttttta ccaccttcac
    35341 cgcgcccacg tcggtcagag cacgatacaa cggcggtaag ttttcaccac gagataccag
    35401 cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc tcttcaatca tctttttgat
    35461 aaaggcgact tcaccacctt cacaccacaa ctcctgttgc tgaccaccaa agttcagtgc
    35521 atcgtctttg ttcagcccca ggttacggcg tttacgctca ctacctgccc gcgctgcagc
    35581 ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt ttttatggat
    35641 gatgccgtta aaaattgccc cactctcttt ttgccgacgc agacgaatgg cgcggttaag
    35701 ccccggatta gcactgataa tcgcctgcgc actgcttaac gcctggctgc tggtttcact
    35761 accggtaaaa cgccagccat attcatgtac gccaatcagc ggataaatac agttcgcacc
    35821 aacgccgata tccagaatgc tggcattcgc tggaattgtt ccgcttgctt cagccagtaa
    35881 atcggcaagg tgatgaatat aatccgcccg gcccggtact ggtgggcaga gaaaaccgtc
    35941 ggggatatcc cagttcgcta cggcgtaaaa atgggccagc aacgccttat tgagcgcctt
    36001 caccgccagc ggattggcaa agtctacgct ttgctccccg gcgggtgtaa gcgtgaggaa
    36061 ttgcctgagt tcaggattga cctgacaaag cgtggcgaga tcgtagcggc tgtgatgacg
    36121 gttgcgcgga tgcaaccccg gtttctgggc ggacatagca ttctccttta agcagcggcg
    36181 taagataccc gcataacgcg acagggtaaa tatctggctg cgtttatcat tccccaaaac
    36241 cttaacatgg ctatggttta tggcaattca tcataaaaat cagcattatg catgtcatta
    36301 atttgtctta ttttgtaaaa atatatcgtt caaaaaataa gcacatctct gtcaattttt
    36361 tgcgtcattt gcatcgcgca ggcgacctac acttatcagg cactacccgt tgtatttatg
    36421 aggatgtcat tatgaaaaag tgcctcacac tactgattgc caccgtcctg agcggaatct
    36481 ctctcacggc ttatgccgcg caaccgatga gtaacctgga cagcggtcag ctacggcccg
    36541 ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga tctggaggat aaactggcag
    36601 aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc
    36661 agatgttcgg tactgcaacc atctacaaat aaccccacca acgtaactgc attcgcctct
    36721 ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaactc aatgtaaata
    36781 aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc gccaagtgat aatgcttatc
    36841 aaaattatta tcacttgcat gaacactatc acgggattaa cagtggcatc gcatccgcag
    36901 agatgctttc tcgtggcagt gaaaatttca acatataaga aaaagtcacc tgcaaaatgg
    36961 aaaacaatcg caatttccct gccagacaat ttcattcgct cacgttcttt gccggtcttt
    37021 gtattggcat cacgcctgtg gctcaggcac tcgccgccga agggcaaact aacgcggatg
    37081 acacgctggt tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa tctgccgatc
    37141 cgaaattctc gcgtccggta gcggatacta cccgcacgat gacggtgatt tctgaacaag
    37201 tgattaaaga tcagggcgca accaacctta ccgacgcgct caaaaacgcc cccggcg
//
LOCUS       DAZPJR010000023        35776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPJR010000023 DAZPJR010000000
VERSION     DAZPJR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.024"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(1..581)
                     /locus_tag="ACHVB7_001087"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="ACHVB7_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="ACHVB7_001088"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="ACHVB7_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR1913142.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVSYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="ACHVB7_001089"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="ACHVB7_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR1913143.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="ACHVB7_001090"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="ACHVB7_001090"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR1913144.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="ACHVB7_001091"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="ACHVB7_001091"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR1913145.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="ACHVB7_001092"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="ACHVB7_001092"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR1913146.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="ACHVB7_001093"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="ACHVB7_001093"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR1913147.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="ACHVB7_001094"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="ACHVB7_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR1913148.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="ACHVB7_001095"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="ACHVB7_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR1913149.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="ACHVB7_001096"
     CDS             complement(10994..11137)
                     /locus_tag="ACHVB7_001096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913150.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="ACHVB7_001097"
     CDS             11138..11416
                     /locus_tag="ACHVB7_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913151.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQSDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13556)
                     /locus_tag="ACHVB7_001098"
     CDS             complement(11463..13556)
                     /locus_tag="ACHVB7_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1913152.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSFDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGEQ
                     VKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEPL
                     GHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMGT
                     ADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNLQ
                     ANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSAK
                     DMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVVG
                     GYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGRK
                     FQLYDMTSDRSVNFR"
     gene            complement(13556..14293)
                     /locus_tag="ACHVB7_001099"
     CDS             complement(13556..14293)
                     /locus_tag="ACHVB7_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1913153.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14290..14928)
                     /locus_tag="ACHVB7_001100"
     CDS             complement(14290..14928)
                     /locus_tag="ACHVB7_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR1913154.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15042..15284)
                     /gene="yjbE"
                     /locus_tag="ACHVB7_001101"
     CDS             complement(15042..15284)
                     /gene="yjbE"
                     /locus_tag="ACHVB7_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR1913155.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15746..17395)
                     /gene="pgi"
                     /locus_tag="ACHVB7_001102"
     CDS             complement(15746..17395)
                     /gene="pgi"
                     /locus_tag="ACHVB7_001102"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR1913156.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      17632..17828
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17920..19269
                     /gene="lysC"
                     /locus_tag="ACHVB7_001103"
     CDS             17920..19269
                     /gene="lysC"
                     /locus_tag="ACHVB7_001103"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR1913157.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19521..19793
                     /locus_tag="ACHVB7_001104"
     CDS             19521..19793
                     /locus_tag="ACHVB7_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR1913158.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19926..20798)
                     /gene="rluF"
                     /locus_tag="ACHVB7_001105"
     CDS             complement(19926..20798)
                     /gene="rluF"
                     /locus_tag="ACHVB7_001105"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR1913159.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21146..21983
                     /locus_tag="ACHVB7_001106"
                     /pseudo
     CDS             21146..21983
                     /locus_tag="ACHVB7_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22053..22859
                     /locus_tag="ACHVB7_001107"
     CDS             22053..22859
                     /locus_tag="ACHVB7_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1913160.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22869..23276
                     /locus_tag="ACHVB7_001108"
     CDS             22869..23276
                     /locus_tag="ACHVB7_001108"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR1913161.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23276..23770
                     /locus_tag="ACHVB7_001109"
                     /pseudo
     CDS             23276..23770
                     /locus_tag="ACHVB7_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23836..24633
                     /locus_tag="ACHVB7_001110"
     CDS             23836..24633
                     /locus_tag="ACHVB7_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR1913162.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24644..25468
                     /locus_tag="ACHVB7_001111"
     CDS             24644..25468
                     /locus_tag="ACHVB7_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR1913163.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25520..26748
                     /gene="sorE"
                     /locus_tag="ACHVB7_001112"
                     /pseudo
     CDS             25520..26748
                     /gene="sorE"
                     /locus_tag="ACHVB7_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27131..27821
                     /gene="pepE"
                     /locus_tag="ACHVB7_001113"
                     /pseudo
     CDS             27131..27821
                     /gene="pepE"
                     /locus_tag="ACHVB7_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
     gene            complement(27912..29543)
                     /locus_tag="ACHVB7_001114"
     CDS             complement(27912..29543)
                     /locus_tag="ACHVB7_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR1913164.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29763..33446)
                     /gene="metH"
                     /locus_tag="ACHVB7_001115"
     CDS             complement(29763..33446)
                     /gene="metH"
                     /locus_tag="ACHVB7_001115"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR1913165.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33646..34470
                     /gene="iclR"
                     /locus_tag="ACHVB7_001116"
     CDS             33646..34470
                     /gene="iclR"
                     /locus_tag="ACHVB7_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR1913166.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34787..35776
                     /gene="espL4"
                     /locus_tag="ACHVB7_001117"
                     /pseudo
     CDS             34787..35776
                     /gene="espL4"
                     /locus_tag="ACHVB7_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8598 a         9231 c         9101 g         8846 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagctaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaatcaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gaaggacggg
    12721 cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt gccacgttca
    12781 tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac gttaaactta
    12841 cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc atactccagt
    12901 ttcaggcgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag tgaggccggg
    12961 ccgtggaaca tctgactgcc gtcgatagat cccgcttgtt tgtagctgtt atcgcgatag
    13021 caatatttat cactggctga acagagcgga tttttcacat taccgctggt gcccagatat
    13081 ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc aacaagatat
    13141 tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac ttgcggcagc
    13201 cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt atacgtttga
    13261 tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt gtaacgcagc
    13321 gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta ctgatcgtta
    13381 tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg agtttgtaat
    13441 aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata tgtttcgccg
    13501 tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt tttcattatt
    13561 ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca ggcgtctcac
    13621 tccagacgga gtctgcgagg ccaacataaa taatgctgcc gggcatcggc tctacgtgac
    13681 gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa acgacccacg
    13741 cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc gccacgtcgc
    13801 gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac gtgaccgtgg
    13861 acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta ccgcgttcgg
    13921 caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact tccaatgcct
    13981 ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa tctgcgccct
    14041 gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc gttgccagct
    14101 cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc cctaccagat
    14161 cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg ctgaacacct
    14221 tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac gcgacaatag
    14281 tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga aataatccgc
    14341 gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt agcggttatg
    14401 ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg tttcatcgct
    14461 gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg tggcgtagcg
    14521 tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat caacaatttg
    14581 tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt tgtcgccgcc
    14641 aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat cctgagtgac
    14701 ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg gcccgccatt
    14761 aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg tcagctgtac
    14821 gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag tcgtggctga
    14881 acaggcctgt aataacaggc agataagaat gagtgcaggt cgcttcacga ctcttctcca
    14941 gaggggcaga ataaccacac cggagtgtgg ttatggttaa tcccttcata tttcaagcca
    15001 cagatgcaat gactcgaatt attttgggga tacatacttt attactgggt actggtggtt
    15061 gtggttgttg tggtcccggt attagaacca tcaccgccac cggttgccgc gagcgcgaca
    15121 cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc cgaaaccgac
    15181 gttgctgccg cccctgccgc ttcgcccacc tgtacaggtg cagcccacac agaagtcgcc
    15241 gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat ttcccttcat
    15301 tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac acaactaaag
    15361 catgcgaatt acggcgcggg gaattagcga tggtgtttta ggataattgg aatgaatatc
    15421 aaatataagt ttcaacttat atttaaataa caataaaaat cataaactta aaataaaaac
    15481 aaaagtaatt aatctgcgta ttctccgaaa taaacaacga ttaaaaatct attttaggat
    15541 taacctcacg gtatgatttc cgttaaatca cagacaaggg gatttatctg ataaaacaat
    15601 gccgacttat tgtcggcatt gtttttaagg ttatttaact cctcatacta tgcattaatg
    15661 tcgatattgg catcaggctc cggttgccgg atgcggcgta aacgccttat ccggcctaca
    15721 tatcggcatc aggcacgcat gatgattaac cgcgccacgc tttatagcgg ttaatcagac
    15781 cattggtcga gctatcgtgg ctgctgattt ctttgtcatc tttcagctct ggcagaatac
    15841 ggttcgccag ttgtttaccc aactccacgc cccactggtc gaaggtaaag atgttcagga
    15901 tcacgccctg agtaaagatt ttgtgctcat acagcgcaat caacgcaccc aggctgaacg
    15961 gagtgatttc gcgcagcagg atggagttgg tcgggcggtt accttcgaac actttgaacg
    16021 gcaccacgta gtcaagcgtt gccggatctt taccctgatc gcgatattcc tgctcaacca
    16081 cttcgcggga tttaccaaac gccagcgctt cggtctgggc gaagaagtta gacagcagtt
    16141 tctggtggtg atcagagagc gggttatggg tgatagccgg ggcgatgaaa tcgcacggta
    16201 ccattttggt tccctggtgg atcagctggt agaacgcgtg ctgaccgtta gtgcctggtt
    16261 caccccagat aatcgggcca gtctggtaat ccacaacgtt accgttacgg tcaacatact
    16321 taccgttgga ctccatattg ccctgctgga agtacgccgc gaaacggtgc atatactggt
    16381 catacggcag aatcgcttca gtttccgcac caaagaaatt gttgtaccag atgccaatca
    16441 gcgccagcag gacaggcagg tttttctcgg caggcgtggt ggagaaatgc ttgtccatcg
    16501 cgtgtgcgcc agaaagcagt tcaacgaagt tatcaaagcc gatggagaga acaatcgaca
    16561 ggccaatcgc tgaccacaaa gagtaacggc cgccgaccca gtcccagaac tcgaacatgt
    16621 tggcagtatc aataccaaac tcgccaacgg ctttggcatt ggtggaaagc gccgcaaagt
    16681 gttttgcaac gtgtttttca tcacctgccg ctttcaggaa ccagtcacgc gcgctatggg
    16741 cgttggtcat agtttcctga gtggtgaagg ttttagatgc taccaggaac agcgtggttt
    16801 ccgggtttac ttttttcagc acttcagcga tgtgagtccc atcgacgtta gaaacaaagt
    16861 gcatgttcag gtgatttttg tacggacgca gtgcttcggt caccatgtac gggccgaggt
    16921 cagaaccacc gatcccgatg ttcactacgt cagtgattgc tttgccggta taacccttcc
    16981 actcaccgga gataatcgct tccgagaagg ttttcatctt ctccagcacc gcgttgactt
    17041 ccggcattac gtctttgcca tcaaccagaa tcggggtatt gctacggtta cgcagcgcta
    17101 cgtgcagcac ggcgcggttt tcagtgcggt tgatcttctc gccagagaac atcgacttaa
    17161 tcgcgcccgc cagatcgcac tctttcgcca gatcctgtaa tttcgccagc gtctcttcag
    17221 tgatgcggtt tttggagtaa tccaccagca tctgatcgtc gaatgttgcg gagaacttag
    17281 aaaaacgatc gccgtcttta gcaaaaagat cggcgatcgt aacgtctttc atttcatcga
    17341 agtgtttctg tagtgcctgc caggcagcgg tctgcgttgg attgatgttt ttcattagca
    17401 atactcttct gattttgaga attgtgactt tggaagattg tagcgccagt cacagaaaaa
    17461 tgtgatggtt ttagtgccgt tagcgtaatg ttgagtgtaa acccttagcg cagtgaagca
    17521 tttattagct gaactactga ccgccaggag tggatgaaaa atccgcatga ccccgtcgtt
    17581 gacaaccgcc ccgctcaccc tttatttata aatgtactac ctgcgctagc gcaggccaga
    17641 agaggcgcgt tgcccaagta acggtgttgg aggagccagt cctgtgataa cacctgaggg
    17701 ggtgcatcgc cgaggtgatt gaacggctgg ccacgttcat catcggctac aggggctgaa
    17761 tcccctgggt tgtcaccaga agcgttcgca gtcgggcgtt tcgcaagtgg tggagcactt
    17821 ctgggtgaaa atagtagcga agtatcgctc tgcgcccacc cgtcttccgc tcttcccttg
    17881 tgccaaggct gaaaatggat cccctgacac gaggtagtta tgtctgaaat tgttgtctcc
    17941 aaatttggcg gtaccagcgt agctgatttt gacgccatga accgcagcgc tgatattgtg
    18001 ctttctgatg ccaacgtgcg tttagtagtc ctctcggctt ctgctggtat cactaatctg
    18061 ctggtcgctt tagcggaagg gctggaacct ggcgagcgat tcgaaaaact cgacgctatt
    18121 cgcaacatcc agtttgccat tctggaacgt ctgcgttacc cgaacgttat ccgtgaagag
    18181 attgaacgtc tgctggagaa cattactgtt ctggcagaag cggcggcgct ggcaacgtct
    18241 ccggcgctga cagatgagct ggtcagccac ggcgagctga tgtcgaccct gctgtttgtc
    18301 gaaatcctgc gcgaacgcga tgttcaggca cagtggtttg atgtacgtaa agtgatgcgt
    18361 accaacgacc gatttggtcg tgcagagcca gatgtagccg cgctggcgga actggccgcg
    18421 ctgcagctgc tcccacgcct caatgaaggc ttagtgatca cccagggatt tatcggtagc
    18481 gaaaataaag gtcgtacaac gacgcttggc cgtggaggca gcgattatac ggcagccttg
    18541 ctggcggagg ctttacacgc atctcgtgtt gatatctgga ccgacgtccc gggcatctac
    18601 accaccgatc cacgcgtagt ttccgcagca aaacgcattg atgaaatcgc gtttgccgaa
    18661 gcggcagaga tggcaacttt tggtgcaaaa gtactgcatc cggcaacgtt gctacccgca
    18721 gtacgcagcg atatcccggt ctttgtcggc tccagcaaag acccacgcgc aggtggtacg
    18781 ctggtgtgca ataaaactga aaatccgccg ctgttccgcg cgctggcgct tcgtcgcaat
    18841 cagactctgc tcactttgca cagcctgaat atgctgcatt ctcgcggttt cctcgcggaa
    18901 gttttcggca tcctcgcgcg gcataatatt tcggtagact taatcaccac gtcagaagtg
    18961 agcgtggcat taacccttga taccaccggt tcaacctcca ctggcgatac gttgctgacg
    19021 caatctctgc tgatggagct ttccgcactg tgccgggtgg aagtagaaga aggtctggcg
    19081 ctggtcgcgt tgattggcaa tgacctgtca aaagcctgcg gcgttggcaa agaggtattc
    19141 ggcgtactgg aaccgttcaa cattcgcatg atttgttacg gcgcatccag ccataacctg
    19201 tgcttcctgg tgcccggcga agatgccgag caggtggtgc aaaaactgca tagtaatttg
    19261 tttgagtaaa tactgtatgg cctggaagct atatttcggg ccgtattgat tttcttgtca
    19321 ctatgcttat caataaacga gcctgtactc tgttaaccaa cgtctttatc gagaataatt
    19381 gcctttaatt tttttatctg catctctaat taattatcga aagagataaa tagttaagag
    19441 aaggcaaaat gaatattatc agttctgctc gcaaacgaat tcccgtacac tgaactctct
    19501 ggctcactga gggcaccatc atggcactcc cccgtattac ccaaaaagag atgactgaac
    19561 gcgagcagcg tgaacttaaa acactgctgg atcgggcgcg gattgcgcat ggtcgcgtac
    19621 tgaccaactc cgaaaccaac agcattaaga aagagtacat cgataaatta atggtcgagc
    19681 gtgaggctga agcgaaaaaa gcccgccaat tgaagaaaaa gcaggcttat aaaccggatc
    19741 ctgaagcgtc atttagctgg tcagcaaata cgtcgacgcg cggcaggcgc taatgaacaa
    19801 tcaacgcctg agattttcac tcgtcagtgt gaacacaatt ttcccctcac cctaaccctt
    19861 ttcccgaagg ggcgagggga ctgtccgagc acttttgggc cgttttcatc agtctgaggc
    19921 gttaatcagc gccccttctt tttacgcccc ggcgaggtaa aacgcttacc gttggacgcc
    19981 gggcgaccgc ctttttccgc cgttttttcc atcttaacga ctggacgttt gatgcccgct
    20041 gttttcggtt tggccttcgc tttgggcttc gcctcggaag aggaattttc aatgagctta
    20101 aagagatcga taagctcgtc gtcggttaaa tcgcgccatt ctcccagcgg aatgccgctt
    20161 aagctgacgt tcataatgcg cgtacgttcc agctttttca cttcatagcc gaaatgctcg
    20221 cacatgcgcc ggatctgacg gttcaacccc tgcaccaggg taatgcggaa gacaaacggt
    20281 gcttcttttt tgactttgca ctttttcgtc accgtcccga ggatcggcac tcccgccccc
    20341 ataccacgaa taaattcatc ggtaatcggt ttatcgaccg tcaccagata ctctttctca
    20401 tgatcgttac ccgcacggag gatcttattc accagatcgc cgtgattggt gaggaaaatc
    20461 agcccctggg agtctttatc caggcggcca atcgggaaca cgcgtttgct gtggttaacg
    20521 aaatcgacaa tgttatcgcg ctcgccatct tcggtggtgc tgacaatacc gacgggcttg
    20581 ttcagggcga taagtaccaa atcttcggct tcccgaggct caatcaactg accgtttact
    20641 ttcacaatgt cgccgggttt cacctgatca ccaatggtgg ctcgcttgcc attaaggaac
    20701 acattgcctt gctcgatata gcgatccgct tcgcggcgtg agcaaattcc gctttcgctg
    20761 atgtatttat ttaaacggac tgatgagtcg ggcagcataa attctcctgt aaaacgcgga
    20821 atataccgta cctcttagcg gggaaaaaca gggttatgcg gcaacgtcga aattaaatgt
    20881 gatctgccac gccttttatc acaaaagtgt ctccggaagt acgatgcaaa tttgcctggc
    20941 aaaataagga taatcaacat attacgaatc gctgcacacc tgtgctgatg actactacgc
    21001 agagaaacaa atgtgcaaga gaaagcgcta tttctcccca tgcaaagagt gaaaaatcgc
    21061 tttgaaaggc cctcataatg gggttatatc agatcaagcg gtgagaaagc gtatttgcac
    21121 aaatgtgcag aaccaggagg cggcaatgga aaacagtgac gatatccgtt tgattgtgaa
    21181 gattgcccaa ctctattacg aacaggatat gacgcaggcg caaatcgcgc gcgaactggg
    21241 tatttaccgc accaccatca gccgcttgct taaacgaggc cgcgatcagg gaattgtcac
    21301 catcgccatc aactatgact acaacgaaaa tctctggctg gagcagcaac tgaagcaaaa
    21361 gtttggcttg aaagacgttg tggtggtgtc gggaaatgat gaggatgaag agactcaact
    21421 ggcgatgatg gggttacacg gcgcgcaact gctggatcgc ttgctggagc ctggcgatat
    21481 tgtcggtttt tcctagggtc gcgcggtgag cgcactggtt gaaaacttgc cgcaagcggg
    21541 gcaatcgcgg cagttaatct gcgtgccaat tattggcgga ccgtccggta aactcgaaag
    21601 ccgctatcac gtaaacacat taacctacag cgtggcagcg aagctgaaag gggaatcgca
    21661 tctcgcggat tttccggctc tgctggataa ccccttaatt cgtaatggga tcatgcagtc
    21721 tcagcacttt aaaaccatct ctgcctactg ggataatctg gatgtcgccc tggtgggaat
    21781 tggctcaccg gccattcgcg acggcgctaa ctggcatgcg ttttatggtg gtgaagagag
    21841 tgacgacctg aaagcaggcg cggtattcca ttggcattgc catgagtgaa gaaaaataca
    21901 gcggaattgt tggtgcactg cgtggaaaat atattaattg tctggtaacg aatagcagca
    21961 cagctgaact attactgaaa taaaaatctt cccgatgttt accgtaataa aaattacggc
    22021 gggtccctat tatctaaaat caggagtctg ttatgcaaac gtggttaaat ttgcaggata
    22081 aaatcattat cgtcaccggc ggcgcatccg gtattggtct ggcgattgta gaggaattat
    22141 tagcacaagg cgcaaacgta cagatggtag atattcacgg tggcgatggt caatatgaag
    22201 ttcataaagg ctatcagttt tggccgaccg atatttccag cgccaaagag gtaaatcata
    22261 cggtagcgga aattatccag cgttttggtc gcatcgacgg tctggtcaat aacgccgggg
    22321 tcaatttccc gcgtctgctg gtcgatgaga aagcgcctgc cgggcagtat gaactcaacg
    22381 aagctgcatt cgaaaaaatg gtcaatatca accaaaaagg cgtttttctg atgtcgcagg
    22441 cggtggcgcg ccagatggtc aaacaacatg atggcgtgat tgtgaatgtt tcctcagaaa
    22501 gtggactgga aggctcagaa ggccaaagct gttacgccgc taccaaagcc gcgctcaata
    22561 gcttcacgcg ctcctggtcg aaagagctgg gtaaacacgg tatccgtgtg gtcggtatcg
    22621 cgccggggat tctggaaaaa accgggctac gcacgccgga atatgaagag gcgctggcgt
    22681 ggacgcgcaa tatcaccgtc gagcagctgc gtgaaggcta taccaaaaac gccattccta
    22741 ttgggcgcgc cggaagatta gcagaagtcg ctgattttgt ttgttatctg ctgtctgaac
    22801 gcgccagcta catcaccgga gtaaccacta acattgcggg cggcaaaacg cgcggataag
    22861 gaggctttat ggtcaacgcc attttttgtg cccacggcaa attagcttgc gccatgctgg
    22921 aatcggttca gatggtgtac ggcgacgcca atgtcgaggc ggttgaattt gtaccaggcg
    22981 agaacgccgg agacatcgta gcaaagctgg aaaagttagt aagcattcac aatcaagatg
    23041 agtggctgat tgccgtggat ttacagtgcg gcagcccgtg gaatgcggca gcgatgcttg
    23101 cgatgcgcaa tcctcgtcta cgggtaatca gcggcctttc cctgccgctg gcgctcgaac
    23161 tggttgataa ccaggacagc atgaatgttg atgaactgtg tgaacacctg acgcaaatcg
    23221 caaagcaaac ctgcgttgtc tggaaacaac tggcaacggc agaggaagat ttctgatgaa
    23281 cattacgctc gcccgcattg atgaccgtct gatccacggc caggtcacta ccgtctggtc
    23341 gaaagtggct aacgcccagc gcattattat ctgcaatgac gaagtttata acgatgaagt
    23401 gcgccggaca ttattgcgtc aggctgcgcc accgggaatg aaggttaacg tggtcaatat
    23461 tgaaaaagcc gtcgcggttt atcacaaccc gcaatattag gacgaaaccg ttttttatct
    23521 tttcactcgc ccacaggatg ccttagcgat ggttcgccag ggggtaaaaa taggcaccct
    23581 gaatattggc ggcatggcct ggcggcccgg taaaaaacag ttaaccaaag cggtttcatt
    23641 agatgatgat gatattaacg catttcatga gttaaataat cttggcgtca taatggattt
    23701 acgcgtcgta gcgtcagatc cctcaatcaa tattatcgac aaaataaacg aacagttaat
    23761 cgcaaattaa ataatagcgc ctgtgtacat atgctttaac aggcagggat gacgacactt
    23821 aaaaggtgac aaatcatgga aataagtacc ctacaaatta ttgctatatt tcttttttcc
    23881 tgtattgccg gaatgggcag cgtgctggat gaatttcaga ctcatcgccc gttaattgcc
    23941 tgtacggtga ttggtttaat tctcggtgat ttaaaaaccg gaataatact cggtggtacg
    24001 ctggaattga tagctctcgg ctggatgaac gtcggcgcgg cgcaatctcc ggattctgca
    24061 ctcgccagca taatctccgc cattctggtt atcgttggtc agcaaagcat cgccaccgga
    24121 atcgccatcg cgttgcctgt ggctgcggca ggccaagtgc tgacggtgtt tgcccgtacc
    24181 atcaccgtag tgttccagca cgcggcggat aaagcagcgg aagaggcgcg gtttcgcact
    24241 ctcgatattc tgcatgtctc cgcgcttggc gtgcaggcgc tgcgcgttgc tattccggca
    24301 ctgattgtct cactgttcgt cagcgccgat atggtcagca atatgttaaa cgccattccc
    24361 gaatttgtga cccgtggact gcaaattgct ggcggtttta tcgtggtggt cggttacgcc
    24421 atggtgctac gtatgatggg cgtgaaatat ttgatgcctt tctttttcct cggtttcctc
    24481 gcaggtggct acctcgatct cagcctgctg gcgttcggtg gcgtcggcgt gatcatggcc
    24541 ctggtctaca tccagctaaa tccacagtgg cgtaaagctg aacctcaact ccaggccacc
    24601 gcctccaccg cccttgacca acttgacgat taacggagcc atcatggaac agagaaaaat
    24661 tacacgcagc gatctggtga gcatgtttct gcgctccaac ctgcaacagg cgtcctttaa
    24721 ctttgaacgt attcacggac tgggcttttg ctacgacatg atccccgcca tcaagcgact
    24781 ttacccatta aaagaggatc aggttgcggc gctcaggcga cacctggtgt tcttcaatac
    24841 cacgccagcc gtatgtggcc cggtcatcgg cgtcactgcc gccatggaag aggcgcgggc
    24901 caacggcgcg gaaattgatg acggtaccat taacggcatc aaagtcggtc tgatgggacc
    24961 attggcagga gttggcgatc cactggtctg gggaacgctg cgcccgatta ccgctgcgct
    25021 cggcgcatct ctggcacttt ctggcaacat cctcggcccg ctgctgtttt tctttatttt
    25081 caatgcggtg cgtctggcga tgaagtggta tggcctacag ctcggctttc gcaaaggggt
    25141 gaatatcgtc agcgatatgg gcgggaatgt gctgcaaaaa ctcaccgaag gcgcgtcgat
    25201 tctcggactg tttgtgatgg gcgtgctggt caccaaatgg acgtcaatca acgtaccgtt
    25261 ggtggtttca caaacgcatg ccgccgatgg ctccaccgtc atcatgaccg tgcagaacat
    25321 tctcgaccaa ctttgccctg gtttgctggc gctcggtctg acgctactga tggttcgtct
    25381 gctcaacaaa aaaattaacc cggtatggct gattttcgcc ctgtttggct tagggattat
    25441 cggcaatgcg ctgggcttcc tgtcctgatt cttcgccccg gcacgactgc cggggccatc
    25501 gctcaacatg aggtggttta tgaaaacaac agctctgcgt ctttatggta aacgtgattt
    25561 acgcctggaa acctttgacc ttcctgaaat gcaggaggat gaaatcctcg cgacggtggt
    25621 cactgacagc ctgtgcctct cttcctggaa agaggcaacc tgggtgaaaa ccataaaaaa
    25681 gtacccgacg atgtggcgac caacccaatc atcatcggcc acgagttttg cggcgatatt
    25741 ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg gtcagcgtta tgtgattcag
    25801 gccaacctga aactccctga ccgcccggac tgccccggct actccttccc gtgggtgggc
    25861 ggcgaagcca cgcatgtggt tattcccaac gaggtcatgg aacaagattg cctgctggca
    25921 tacgacggcg aaacctattt tgaaggctcg ctggttgaac cgctttcctg cgtgattggc
    25981 gcgttcaacg ccaactatca tcttcaggaa ggtagttata accacacgat ggggattcgc
    26041 ccgcaagggc gcacgctgat cctcggcggc accggaccaa tgggattgtt ggcgattgat
    26101 tatgcgctgc atggaccagt taacccgtcg ctgctcgtca ttaccgatac cgacaacgac
    26161 aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc aaacactgat tcattatctc
    26221 aatgccaccg atgcagcatt tgatacgtta atggcgctga gtggcggtca cggcttcgat
    26281 gatattttcg tctttgtgcc taatgaagga ctggtgactc tcgcctcttc cttgctggcg
    26341 acagatggtt gcctgaattt cttcgccgga ccgcaggata aacatttcag cgcgccaatt
    26401 aatttctacg atgtgcatta tgcatttacc cactacgtgg gcacgtcagg cggcaatacc
    26461 gacgacatgc gcgcggcggt caaactgatt gaagagaaaa aagtgcaggc cgcaaaagtg
    26521 gtaacacata ttcttgggct gaatgccgcg ggcgaaacca cgcttgaatt gcctgccgtc
    26581 ggcggcggca aaaagctggt gtataccggg aaatacctgc cgctgacgtc actcacgcag
    26641 attcaggatc aagcactggc ggcgattctg gcgcgtcatc aggggatctg gtccggtgag
    26701 gcggagcaat atctgctcac tcatgcagag gcaatttccc atgattaatc gcgctactta
    26761 actctgcatg tcgctggctg gtcgccgcgg caacttcgcg acctgatgtg cggctgattg
    26821 ctgaagcggc ccaatgtgca cgagaaggat aaaaaaacat cagcgcctta aggggcgctc
    26881 aaattggtgg caaagtacac attgtttatg ccggatgcgg aataaagcct tattcggcct
    26941 acataatctc gaaaattcaa tatattgcag ttatctggta ggcctggtaa gcgtagcgca
    27001 tcaggcaatg ctgtgtttgt catcagtctc agcgcccggt gcaatacgtg acatcgtgag
    27061 ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc ttccgtgatt tcttactaac
    27121 cggagaagta atggaactgc ttttattgag taactcgacg ctgccgggta aagcctggct
    27181 ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt cgccgctcag cggtgtttat
    27241 ccctttcgct ggcgtaacgc agacctgtga tgattacaca gcgaaaacgg ctgcggttct
    27301 cgctccgctg ggtgtttctg tcaccggtat tcatagcgtt gtcgatcccg ttgccgcgat
    27361 tgaaaatgct gagatcgtga ttgtcggggg gcgggaatac tttccagttg ctgaaacagt
    27421 gccgcgagcg cgggctgctg gcaccaatta ctgacgtggt taaacgtggt gcgctgtata
    27481 ttggctggag cgcaggcgct aaccttgctt gcccaaccat tcgtaccacc aacgatatgc
    27541 cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt cccgctgcaa atcaacccgc
    27601 acttcaccaa cgcgctgccg gaaggccata aaggtgaaac ccgtgagcag cgtattcgcg
    27661 aactgctggt cgtcgcgcca gaactgacga ttattggtct accggaaggt aactggatca
    27721 cggtgagtaa aggtcacgct acgctgggtg gcccgaacac cacttatgtg tttaaggccg
    27781 gtgaagaagc ggtgccgctg gaagcgggtc accgttttta agtgaattgc cggatgcatg
    27841 atccggcatc ttgttcatag tgcctgatgc gacgcttgac gcgtcttatc aggcctacag
    27901 gattcacaat attaatactc gtcccgtcct tcatcttcat ccggctgttc cagcacactg
    27961 taagccaccg aacaaaacag cgagttcaga cgctgcatat ctcccagtaa gcctaaatgt
    28021 agcgaactgg tttcaatgct ctgcacgttt tgctgatgca ggcgatcgac gtgggcgtgg
    28081 gaatagcggc gattaagaat gcgaaagcga tgcttgctgc ggcgcaaacg acgagcgctg
    28141 gtgacatcgc cagagaagaa caccgacatt gccagcttta aattactgag caattgctca
    28201 tagagcgcat ccagttcctt caacccatca agcgaaaatg cccgccgtgc tgccagcgat
    28261 ttatcagcaa tttcgctgcc catgcgctcg acgatatcgg aagcctgttc aaggttgagc
    28321 gacatttcaa taatctccgc ccagcgacgc gactcttctt ctgccagttc ctcttttggc
    28381 attcgcgcca gatacagctt aatggcggtg tagagaacgt tgatatcatc tgccagctta
    28441 cgcagctctt tctcctgccg tggctcgccg tgcatcactt tattcagccc ttccatcatc
    28501 tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg cgttcgccag agcaagcgtg
    28561 ggcgtatcca gcgcgctgac atccagatgt ttaggccgta gctgggtatc cagttccggt
    28621 tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt caacaaatgg cagcatgacc
    28681 aggcaacgta caaggttgta gaagacgtgg aaatagatca ccagttccgc ttttggcaat
    28741 gacaacttcc ccattgtctc tgccagcaaa tggacaaacg gcaggataat caggctaccc
    28801 accagcttaa acagcaaact acccagcgcg acacggcggg ctgcggcatt ggcggcactg
    28861 ttgttgagca tcgccagcag gccggaaccg aggttagcac caatcaccag acagagcgcc
    28921 acggggaagg agataatgcc tgcggcggtc agagtcgcgg tcagcagtac agcagcaagg
    28981 ctggagtagc tgataatggc gaacatcgcg ccaatcagcg catccagcag aatatcgccg
    29041 gtcagcgagg caaagatcac ctgaacgccg tttgcctggg tgatcggcgt tacggcctgc
    29101 acaatcaact ccagcgccag caaaatcagc ccaagaccaa taccgacgcg gcccagttgc
    29161 ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc cgataaaaat aagtaacggt
    29221 gacagccagg ataagtcgaa ggtgagaata cgcgccatta gcgccgtccc gacatctgca
    29281 cccagcacaa tcaccagagc cggtgcgagg gctaccagat cctgagcgac gaacgaggtc
    29341 accagcatgg tggtggcatt actgctctgt accagtgcgg taacgccgat ccccgcgcaa
    29401 aaggcgagcg gctttttttc gacgctccgg ctaaggacag tacgcaaacg cgcgccgaag
    29461 acgcgcatta cgccggttcg aacaatatga gtcccccaga ccagcagggc gacggcagaa
    29521 agcaggtgaa gcagcgttaa cacgagttca ggttctcctt atcgttatat gttcctgaag
    29581 caattgaatg accaaaggac cggaaacacg ctgactggca tctttactgt gccttttgac
    29641 attcgttttt ccggctaaga ttattccagc ctgtttccag tataagtgtt tcatattaat
    29701 aaagagacag ggcactcatt gagcgccctg tttgttgtaa ggaaaagtga cagatttgtg
    29761 aatcagtccg cgtcataccc cagattcggt gccagccagc gctcaacatc ggagacgctc
    29821 atacctttac ggcgggcata gtcttcaacc tgatcgcgct gaatttgcgc caccgcgtag
    29881 tacttgctgt ccgggtggct gaaataccaa cccgaaaccg atgcaccagg ccacatggcg
    29941 aaagattctg tgagtttcat gccagtatgt ttttccactt ccagcagctc ccagatggtg
    30001 gctttttccg tatgttccgg gcaggccggg tagcccggtg ccggacggat gccctggtag
    30061 ttttcgcgga tcagttcttc gttgctgagg ttctcgttcg gcgcatagcc ccagtagact
    30121 ttacgcacac gctcatggag atactccgca aaggcttcgg ctaaacggtc ggcaagcgct
    30181 ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa aggcatcagc cagtgcgtcc
    30241 tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat ctgctttacc agaaagcttc
    30301 ggcgcaacga agtcagcgag acagtagtta gcgaatcccg ttttttcggt ctgttgacgc
    30361 agatggtggc tgacgttgat cacatgggtg cgcgtttcgt cacggtagat ttcaatgtca
    30421 tcgcccacac ggtttgccgg gaacaagccc accacgccac gcggattcag cgttttctcg
    30481 gcgcttaatt tatccagcat gtcgttggcg tctttaaaca gccgctgcgc ctcaacgccc
    30541 accacttcat cttccagaat gcgcggatac ttcccggcca gcgaccaggt cataaagaac
    30601 ggtgtccagt cgatgtaatt acgcagagtt tcgatgctgg cttcaacttc ctgcacgccg
    30661 agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa aagcgaagtc gttatcgcgc
    30721 gccgcttcca gcgtgaccgg tggtgtgcgc ggtttcttgc gcccgtgctg aatacgtacg
    30781 gtttcatact ccttgcgggt acgagcgaca aaatcatcgc gctgagtatc ggaaagcagc
    30841 gccgccacca caccaacggt gcgcgaggca ttctgcacat acaccgtcgg gccgctgtag
    30901 ttctgctcaa ttttcaccgc cgtgtgcgct tttgaggtcg tcgcgccacc gatcagtaac
    30961 ggaatggtga agccctgacg ctccatctct ttcgccacgt taaccatctc gtccagcgac
    31021 ggcgtgataa gccccgaaag tccaatcaga tcagcattca cttctttagc ggtacggaga
    31081 attttttccg caggcaccat aacgccgaga tcgacaattt cgtagttgtt acattgcagc
    31141 accacaccaa cgatattttt accgatgtcg tggacgtcgc ccttcacggt ggcgatcacc
    31201 atcttgccgt tggttttgcc ctgctctttg ctggcttcaa taaacggttc gaggtaggcc
    31261 actgcctgtt tcatgacgcg cgccgatttg accacctgtg gcaggaacat tttcccctcg
    31321 ccaaacagat cgccgaccac gttcatgccg tccatcaacg ggccttcaat cacttcaatc
    31381 gggcgcgtag cctgctggcg ggcttcttcg gtatcctgct cgataaactc agtaatgcct
    31441 ttgaccagcg agtattccag acgtttattc acttcccacg agcgccactc cgcctgctgg
    31501 gcgttagcgg tgtcgtcggt tttgctgccg cgatatttct cggcaagctc cagtaaacgt
    31561 tcagtgccat catcgcgacg attaagaatc acatcttcca ccgcgtcgcg cagttcagcg
    31621 ggcaggtcgt cgtaaatcgc cagttgcccg gcgttgacga tccccatatc catgccattg
    31681 cgaatagcgt agtagaggaa caccgcgtga atggcttcgc gcaccggttc gttgccacgg
    31741 aatgagaaag aaacgttaga tacgccgccg gaaatcagcg cgtgcggcag ttcgcgtttg
    31801 atgtcttcgc aggcgccgat aaagtcctgc gcatagttgt tgtgctcttc aatgccggtg
    31861 gcgaccgcga agatgttcgg gtcgaagatg atatcttctg gcgggaagcc aacctcttcg
    31921 gtgaggattt tgtacgcccg acggcaaatc tcgattttcc gtgcgcgggt atcggcctgt
    31981 ccctgttcgt caaaggccat taccaccacc gctgcaccgt aacgacgcaa cagtttcgca
    32041 tgatggataa aggcgtcgac gccctctttc attgagatag agttaacaat gcctttgccc
    32101 tgaatacact tcagcccctt ttcgatgacc tcccattttg aggagtcgat cataatcggc
    32161 acgcgagcga tatccggttc accggcaatc agattgagaa aacgcaccat cgccgcttcg
    32221 gcgtcgagca tcccttcatc catattgata tcgataatct gcgcgccgtt ttccacctgc
    32281 tggcgggcca catccagtgc ctcgctgtat ttctcttctt tgatcaggcg cttgaactta
    32341 gcggaaccgg tgacgttggt gcgttcaccc acgttcacaa acaggctatc ttcgccaatg
    32401 ttcagcggct ccaggccgga caaacggcag gctacgggga tttccggcag tttgcgcggc
    32461 gctaatcctt ctactgcacg actcatcgct gcaatatgtt gtggcgtggt gccacagcag
    32521 ccgccgacga tattgagaaa gcccgcttcc gcccattcac gtatttgttt tgccatcgtg
    32581 tcggcgtcga gatcgtactc accaaaggcg ttgggtagcc cggcgttcgg gtgcgcggtg
    32641 acgtagcatt ccgcaatccg tgacagctcc tgcacgtact ggcgcagttc atcgggcccc
    32701 agcgcacagt tcaggccaaa ggtcagcgct tcggcgtggc gcaatgagtt gtaaaatgct
    32761 tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg tgatggtgcc agagatcata
    32821 atcggcagct caacgcccag cgtttcaaac tccgttttta ccgcaaatac cgccgctttg
    32881 gcgttaaggg tgtcgaaaac ggtttcaatc aggatcagat ccgcaccacc ttccaccagc
    32941 gctttggtgg actctcgata agccaccacc agcccgtcaa aagtgatatt acgaaatgcc
    33001 ggatcgttga cgtccggaga aatagacgcc gtgcggttgg ttgggccgag aacaccggca
    33061 acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcgt cagcacaggc gcgcgccagt
    33121 ttcgccgccg caaagttgat ttccgccgac agggattcca tctggtaatc cgccatcgca
    33181 atggtcgtgg agttgaaggt gttggtttcg atgatatccg cgcccgcttc aaagtaggcg
    33241 ttgtggatag cggcgatcac ttccggttta ctgagtacca gcaggtcgtt gttgcctttg
    33301 aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat cggcttcgtt cagtcgatag
    33361 ctctggatca tggtgcccat accgccgtcc agcaccagaa tacgttcatt taactgcgca
    33421 cgcagttgtt ccactttgct gctcacactt gctcccgaca cgctcaaccc agatttaata
    33481 aaaattcaac aaaccatact ggcataaacg catctgtggt aaaagcgacc accacgcaac
    33541 atgagatttg ttcaacatta actcatcgga tcagttcagc aactattgca ttaactaaca
    33601 ataaaaatga aaatgatttc cacgatacag aaaaaggaga ctgtcatggt cgcacccatt
    33661 cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac cagcgactgg acaggttcag
    33721 tctttaacgc gtggcctgaa attactggag tggattgccg aatccaatgg cagtgtggca
    33781 ctcacggaac tggcgcaaca agccgggtta cccaattcca cgacccaccg cctgctaacc
    33841 acgatgcaac agcagggttt cgtgcgtcag gttggcgaac tgggacactg ggcaatcggc
    33901 gcacatgcct ttatggtcgg cagcagcttt ctccagagcc gtaatttgtt agcgattgtt
    33961 caccctatcc tgcgcaatct aatggaagag tctggcgaaa cggtcaatat ggcggtgctt
    34021 gatcaaagcg atcacgaagc gattattatc gatcaggtac agtgtactca cctgatgcgt
    34081 atgtccgcac ctatcggcgg taaattgccg atgcacgctt ccggtgcagg taaagccttt
    34141 ttagcccaac tgagcgaaga acaggtgacg aagctgctgc accgcaaagg gttacatgcc
    34201 tatacccacg caacgctggt gtctcctgtg catttaaaag aagatctcgc ccaaacgcgc
    34261 aaacggggct attcatttga cgatgaggaa catgcactgg ggctacgttg ccttgcagcg
    34321 tgtattttcg atgagcaccg tgaaccgttt gccgcaattt ctatttccgg accgatttca
    34381 cgtattaccg atgaccgcgt gaccgagttt ggcgcgatgg tgattaaagc ggcgaaggaa
    34441 gtgacgctgg cgtacggtgg aatgcgctga ctttttctgg cgggcagagg caatattctg
    34501 cccatcatac ctgagtggca atagaataag ggtgtctgtt aatcgcattg acgccaaaat
    34561 aacttaatgt catacacttc acacactttt attaactcag cattattttt aaacatcaaa
    34621 ccacttaatt attaacaaaa tcgcaagatc tcattccacc gaaactttta cggctgcatt
    34681 atgagattga gcgatttatg atatttcttt agttgtaatc atattaaatt ttttcactgt
    34741 tctgatagtt aaaatttaag acatcaataa acaatgagat atttaaatga ttactcgtat
    34801 tcctcgtagt tctttctctg caaatattaa taatacagcc cagacaaatg aacaccaaac
    34861 cctgagtgaa ttgttttata aagaactcga ggataaattt tctggcaagg agctggcgac
    34921 gcctctatta aaaagcttct cagagaactg tagacaaaat ggtcgtcata tctttagcaa
    34981 caaggatttt gtcattaaat tttccacgtc cgtcttacaa gctgataaga aagaaattac
    35041 gataattaat aaaacgaaaa cacgacactc actcaaacca ttgctccaat atttgaagaa
    35101 tacctaatgg aaattttacc tcaacgctca gacactcttg ataaacaaga attaaaccta
    35161 aaatcagata gaaaagaaaa agaattccca agaattaaac ttaatggtca atgctatttt
    35221 ccggggcgac cccaaaaccg tatagtatgc cgacacattg ctgcacaata tattaatgat
    35281 atttatcaga atgttgatta caaaccccat caagatgatt attcttcagc tgaaaaattt
    35341 ctcactcact tcaacaaaaa atgcaaaaac cagactttgg cgttgatttc cagccgtcct
    35401 gaggggcgtt gcgttgctgc ctgcggtgat ttcgggctag ttatgaaagc atattttgac
    35461 aagatggaat caaatggcat cagtgttatg gcagccatat tactggtgga taaccatgct
    35521 ttgacggtcc ggctaagaat aaagaacaca actgaaggat gtacccatta cgtggtttcg
    35581 gtttatgatc ctaatgtaac taacgacaaa ataagaatca tgagcgaaag caaagaggat
    35641 attaaacact attctctgat ggattttatg aatgtagatt atagcctcct gaaatggtca
    35701 aatgatcatg ttattaacca atctgttgca ataattccag cacttccgaa agaacagcta
    35761 ttgatattaa aaggat
//
LOCUS       DAZPJR010000024        34981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPJR010000024 DAZPJR010000000
VERSION     DAZPJR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.025"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHVB7_001118"
     CDS             complement(<1..815)
                     /locus_tag="ACHVB7_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1913167.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHVB7_001119"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHVB7_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHVB7_001120"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHVB7_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1397..1873)
                     /locus_tag="ACHVB7_001121"
     CDS             complement(1397..1873)
                     /locus_tag="ACHVB7_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR1913168.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHVB7_001122"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHVB7_001122"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR1913169.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="ACHVB7_001123"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="ACHVB7_001123"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR1913170.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="ACHVB7_001124"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="ACHVB7_001124"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR1913171.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="ACHVB7_001125"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="ACHVB7_001125"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR1913172.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="ACHVB7_001126"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="ACHVB7_001126"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1913173.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="ACHVB7_001127"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="ACHVB7_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR1913174.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="ACHVB7_001128"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="ACHVB7_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR1913175.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="ACHVB7_001129"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="ACHVB7_001129"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR1913176.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="ACHVB7_001130"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="ACHVB7_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR1913177.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="ACHVB7_001131"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="ACHVB7_001131"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR1913178.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="ACHVB7_001132"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="ACHVB7_001132"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR1913179.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="ACHVB7_001133"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="ACHVB7_001133"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR1913180.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="ACHVB7_001134"
     CDS             13848..14552
                     /locus_tag="ACHVB7_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1913181.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14823..15470
                     /locus_tag="ACHVB7_001135"
     CDS             14823..15470
                     /locus_tag="ACHVB7_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1913182.1"
                     /translation="MWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLC
                     MYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAG
                     LLFNVEPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNK
                     LLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(15535..16246)
                     /locus_tag="ACHVB7_001136"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="ACHVB7_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="ACHVB7_001137"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="ACHVB7_001137"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR1913183.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="ACHVB7_001138"
     CDS             complement(17484..18245)
                     /locus_tag="ACHVB7_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1913184.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="ACHVB7_001139"
     CDS             18378..18788
                     /locus_tag="ACHVB7_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR1913185.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="ACHVB7_001140"
     CDS             complement(18750..19856)
                     /locus_tag="ACHVB7_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1913186.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="ACHVB7_001141"
     CDS             complement(19867..21000)
                     /locus_tag="ACHVB7_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1913187.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="ACHVB7_001142"
     CDS             complement(20993..22729)
                     /locus_tag="ACHVB7_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1913188.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHVB7_001143"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHVB7_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR1913189.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHVB7_001144"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHVB7_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR1913190.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHVB7_001145"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHVB7_001145"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR1913191.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHVB7_001146"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHVB7_001146"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR1913192.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="ACHVB7_001147"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="ACHVB7_001147"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR1913193.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHVB7_001148"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHVB7_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR1913194.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHVB7_001149"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHVB7_001149"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR1913195.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHVB7_001150"
     CDS             31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHVB7_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR1913196.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHVB7_001151"
     CDS             complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHVB7_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR1913197.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHVB7_001152"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHVB7_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR1913198.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHVB7_001153"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHVB7_001153"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR1913199.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="ACHVB7_001154"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="ACHVB7_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8273 a         8976 c         9488 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAZPJR010000025        34838 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPJR010000025 DAZPJR010000000
VERSION     DAZPJR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.026"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            450..602
                     /gene="mokC"
                     /locus_tag="ACHVB7_001155"
     CDS             450..602
                     /gene="mokC"
                     /locus_tag="ACHVB7_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR1913200.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="ACHVB7_001156"
     CDS             complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="ACHVB7_001156"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR1913201.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="ACHVB7_001157"
     CDS             complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="ACHVB7_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR1913202.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4218..4622
                     /locus_tag="ACHVB7_001158"
     CDS             4218..4622
                     /locus_tag="ACHVB7_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR1913203.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4648..5361
                     /gene="msyB"
                     /locus_tag="ACHVB7_001159"
     CDS             4648..5361
                     /gene="msyB"
                     /locus_tag="ACHVB7_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR1913204.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5510..6076
                     /gene="satP"
                     /locus_tag="ACHVB7_001160"
     CDS             5510..6076
                     /gene="satP"
                     /locus_tag="ACHVB7_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR1913205.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6111..6698)
                     /gene="mog"
                     /locus_tag="ACHVB7_001161"
     CDS             complement(6111..6698)
                     /gene="mog"
                     /locus_tag="ACHVB7_001161"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR1913206.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6813..7766)
                     /gene="tal"
                     /locus_tag="ACHVB7_001162"
     CDS             complement(6813..7766)
                     /gene="tal"
                     /locus_tag="ACHVB7_001162"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1913207.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8045..9474
                     /locus_tag="ACHVB7_001163"
                     /pseudo
     CDS             8045..9474
                     /locus_tag="ACHVB7_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9544..10320
                     /gene="yaaA"
                     /locus_tag="ACHVB7_001164"
     CDS             9544..10320
                     /gene="yaaA"
                     /locus_tag="ACHVB7_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR1913208.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10473..10769)
                     /locus_tag="ACHVB7_001165"
     CDS             complement(10473..10769)
                     /locus_tag="ACHVB7_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR1913209.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="ACHVB7_001166"
     CDS             complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="ACHVB7_001166"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR1913210.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="ACHVB7_001167"
     CDS             complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="ACHVB7_001167"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR1913211.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="ACHVB7_001168"
     CDS             complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="ACHVB7_001168"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR1913212.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15693..15812)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="ACHVB7_001169"
     CDS             complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="ACHVB7_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR1913213.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16027..16713)
                     /locus_tag="ACHVB7_001170"
     CDS             complement(16027..16713)
                     /locus_tag="ACHVB7_001170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1913214.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17113..17253)
                     /gene="yjjY"
                     /locus_tag="ACHVB7_001171"
     CDS             complement(17113..17253)
                     /gene="yjjY"
                     /locus_tag="ACHVB7_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR1913215.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17349..18065
                     /gene="arcA"
                     /locus_tag="ACHVB7_001172"
     CDS             17349..18065
                     /gene="arcA"
                     /locus_tag="ACHVB7_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR1913216.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18125..19477)
                     /gene="creD"
                     /locus_tag="ACHVB7_001173"
     CDS             complement(18125..19477)
                     /gene="creD"
                     /locus_tag="ACHVB7_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR1913217.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19535..20959)
                     /gene="creC"
                     /locus_tag="ACHVB7_001174"
     CDS             complement(19535..20959)
                     /gene="creC"
                     /locus_tag="ACHVB7_001174"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR1913218.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20959..21648)
                     /gene="creB"
                     /locus_tag="ACHVB7_001175"
     CDS             complement(20959..21648)
                     /gene="creB"
                     /locus_tag="ACHVB7_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR1913219.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21661..22134)
                     /gene="creA"
                     /locus_tag="ACHVB7_001176"
     CDS             complement(21661..22134)
                     /gene="creA"
                     /locus_tag="ACHVB7_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR1913220.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22345..23214
                     /gene="robA"
                     /locus_tag="ACHVB7_001177"
     CDS             22345..23214
                     /gene="robA"
                     /locus_tag="ACHVB7_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR1913221.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23211..23858)
                     /gene="gpmB"
                     /locus_tag="ACHVB7_001178"
     CDS             complement(23211..23858)
                     /gene="gpmB"
                     /locus_tag="ACHVB7_001178"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR1913222.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23910..24431
                     /gene="yjjX"
                     /locus_tag="ACHVB7_001179"
     CDS             23910..24431
                     /gene="yjjX"
                     /locus_tag="ACHVB7_001179"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR1913223.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24516..24842)
                     /gene="trpR"
                     /locus_tag="ACHVB7_001180"
     CDS             complement(24516..24842)
                     /gene="trpR"
                     /locus_tag="ACHVB7_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR1913224.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24932..26869)
                     /gene="sltY"
                     /locus_tag="ACHVB7_001181"
     CDS             complement(24932..26869)
                     /gene="sltY"
                     /locus_tag="ACHVB7_001181"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR1913225.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27080..28747
                     /gene="ettA"
                     /locus_tag="ACHVB7_001182"
     CDS             27080..28747
                     /gene="ettA"
                     /locus_tag="ACHVB7_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR1913226.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28908..29015)
                     /gene="nadS"
                     /locus_tag="ACHVB7_001183"
                     /pseudo
     CDS             complement(28908..29015)
                     /gene="nadS"
                     /locus_tag="ACHVB7_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(29054..30286)
                     /gene="nadR"
                     /locus_tag="ACHVB7_001184"
     CDS             complement(29054..30286)
                     /gene="nadR"
                     /locus_tag="ACHVB7_001184"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR1913227.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30307..31689)
                     /gene="radA"
                     /locus_tag="ACHVB7_001185"
     CDS             complement(30307..31689)
                     /gene="radA"
                     /locus_tag="ACHVB7_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR1913228.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31738..32706)
                     /gene="serB"
                     /locus_tag="ACHVB7_001186"
     CDS             complement(31738..32706)
                     /gene="serB"
                     /locus_tag="ACHVB7_001186"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR1913229.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32812..33456
                     /locus_tag="ACHVB7_001187"
     CDS             32812..33456
                     /locus_tag="ACHVB7_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR1913230.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33484..34500
                     /gene="lplA"
                     /locus_tag="ACHVB7_001188"
     CDS             33484..34500
                     /gene="lplA"
                     /locus_tag="ACHVB7_001188"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR1913231.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34532..34795
                     /locus_tag="ACHVB7_001189"
                     /pseudo
     CDS             34532..34795
                     /locus_tag="ACHVB7_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         8266 a         9430 c         8663 g         8479 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa taggcgaggc
      241 gtcttcagga ttagaatcga taaaacagaa tgggttaaag agagcgatat aatagcggcg
      301 ggtgcttgag gctatctgtc tcaggcatta gctgaacggc agatagagaa aagccccgag
      361 tgatatttta ctatcaaccc gaggccccct atatgctgaa cacatgtaga gtgcctctta
      421 ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg attgtcgccc
      481 tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa gacctctgtg
      541 aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac gaatccgagt
      601 aagagcaacg gcggggagtg atccccgcca cttttcaggt gctcgcatat cttcaacgca
      661 ccctaatttt acccaagcct gcccacgggc aggcttttgg ggaggttagc gggtcaggtc
      721 gtcaaaaaac ttcttcacac catcaaagaa gctctttgag cgcgggctgt tgtgctcacc
      781 ggttgggcca ccgaagcttt cttgcagctc ttgcagcaac tgtttctgct tctcgttcag
      841 gcctaccggt gtttcgacga caacgcggca cagcaaatca ccctgtgcgc caccgcggac
      901 agacttgacg cctttaccgc gcatacggaa aagcttaccg gtctgggttt cgccaggcac
      961 tttcagtttg acgcgaccat caagggtcgg tacttcgatt tcgccaccca gcgccgccat
     1021 agcgaagttg atcgggactt cgcaatagag gttgttgcct tcacgctcga aaatcgggtg
     1081 ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt gcgccatgct cgcccgcttc
     1141 accttcgccc gcaagacgga tgcggtctcc agtgtccacc cctgccggga ttttaacgga
     1201 cagcgttttg ctgcgctcaa cacgaccatg accatgacat ttgttgcacg gatctttgat
     1261 cagcgtaccg cggccctgac agtgtggaca ggtctgctgt acagcgaaaa atccctggcg
     1321 catctgcacc tgaccagaac catgacaggt cggacaggtc tgcggctgtg tacctggttt
     1381 tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga tctctttggt
     1441 cacgccacgt acagcttctt cgagggtgag ctccatgtta tagcgtaaat cagcaccgcg
     1501 cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt caccaaaaat
     1561 atcgctgaag tctgcgccgc cgccaaaacc gccgccgccc atgccacctt gctcaaacgc
     1621 agcatgacca tactgatcgt atgccgcacg tttttgcgag tcggtcagaa cttcataagc
     1681 ttccttgatc tctttaaatt tcgcctcggc ctctttgtca ccctggttac ggtccgggtg
     1741 gtatttcatg gccaggcgtt tgtaggcctt tttgatttca cgctcttccg ctgttttgga
     1801 aacgcctaaa atctcgtaat aatcttgctt agccatcttt tttaaattgc ccctagatga
     1861 atgcacgggc ggagaggaaa ttccccttcg cccgtgtcag tataattacc cgtttatagg
     1921 gcgattattt tttgtctttg acttcttcaa attcagcgtc gacaacatcg tcatctttcg
     1981 cgttgtttgc agaagcatca gcaccggcag tctgctgctg ggcatgttgc tgctgggcga
     2041 tttccatcag tttctgggaa acctgtgcca gttcctgcat tttcgcttcg atagcggctt
     2101 tgtcttcacc tttcagagca gtttccagtg cagtcagcgc agactcgata gcagttttgt
     2161 cgtcagccgg cagtttgtcg cctgcttctt caacctgctt acgggtgctg tgcagcagat
     2221 ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt acggtcagct tcggcgttag
     2281 cttctgcgtc gcgtaccatt ttctggattt catcttcgtt cagaccagaa gaagccttga
     2341 tggtgatctt ctgctcttta ccgctgtttt tatctttcgc ggaaacgtgc aagataccgt
     2401 cagcatcgat atcgaaggta acttcgatct gcggcatgcc gcgcggtgcc gggttgatac
     2461 catccaggtt gaactgaccc agagatttgt tatcagctgc acgtttacgt tcaccctgca
     2521 gcacatggat ggttaccgca gactggttgt cttcagcggt agagaacacc tggctgtgct
     2581 tggtcgggat agtggtattt ttcgcgatca gcgtcgtcat cacaccgccc atggtttcga
     2641 tacccagaga cagcggggta acgtccagca gcagtacgtc ttttacgtca ccagtcagaa
     2701 caccaccctg aacagcagca ccgattgcta cagcttcgtc cgggttaacg tctttacgcg
     2761 gctctttacc aaagaactca gcaactttct tctgaaccat tggcatacga gtctgaccac
     2821 ccacgaggat aacgtcgtcg atatcagata cggacaggcc agcgtcctgc agtgcaactt
     2881 tcagcggctc aatggaacgg tttaccagat cttcaaccag gctttccagt ttcgcacgag
     2941 tcactttgat gttcatgtgt ttcggaccgg tcgcatctgc agtgatgtac ggcaggttaa
     3001 cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt ttctgccgct tctttcaggc
     3061 gctgcattgc cagcggatcg ttgcgcaggt caatgccctg atctttcttg aattcttcaa
     3121 ccaggtagtt gatcagacgg ctgtcgaagt cttcaccccc caggtgggta tcaccgttgg
     3181 tagccagaac ttcgaaggtt ttttcgccgt caacttcgtc gatttcgata atagaaatat
     3241 cgaaagtacc accacccagg tcataaaccg cgatagtacg gttgccagta cctttgtcca
     3301 gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat acgttttact tccagaccag
     3361 cgatacggcc tgcgtctttg gttgcctgac gctgagcatc gttaaagtat gccggtacgg
     3421 tgataacagc ttcagttacc ggttcaccca agtaatcttc agcggttttc ttcatttttt
     3481 tcagcacttc agcagaaatc tgcggcggtg ccattttctg gcctttaact tcgacccatg
     3541 cgtcgccgtt atcagcagca ataattttga acggcatgat ggaaacatca cgctgtactt
     3601 cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc aaacagggtg ttttgcgggt
     3661 tcgtcactgc ctgacgttta gccggctgac caaccagagt ttcaccatcc tgggtatagg
     3721 caataataga aggcgtggtg cgatcgcctt cggcgttctc cagcacgcga ggagtggtgc
     3781 catccataat cgctacacaa gagttggtag tacccaggtc gataccaatt attttaccca
     3841 tctaaacgtc tccactatat attcggtcat catgtggttg tgagtctgta ataggggcga
     3901 aacgtctggt ttcaactgcc caatttcatt tttttgcaga ctcactcact gcggttgact
     3961 actaaatggg gtcgtaaccc acgtcatcaa gggggagatg caaaaatttt ttgtgctttt
     4021 gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat tgacagcaat tttattcata
     4081 taaagaatga atcgcagcca aggcattcat caaaaaattg taataaaaag aaaagattac
     4141 gtacctgaat cttctcttta tcagcagtaa actagtgggt attcatccct ctacctcttc
     4201 ccactaagag aatccttatg aaatccgttt ttacgatttc cgccagcctg gcgattagcc
     4261 tgatgctgtg ctgcacggcg caggcaaacg accataaact cctcggcgtc attgcaatgc
     4321 cgcgtaacga aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt gtgaaacgca
     4381 tacaactctc tgccgaccat ggcgatttac agttaagcgg tgcatcggtt tatttcaaag
     4441 ccgcccgtag cgccagtcag agtctgaata ttccttcaga aataaaagaa gggcaaacca
     4501 ctgactggat caacattaac agtgataacg acaataaacg ctgcgtctca aaaatcacct
     4561 tttccggtca tacggtgaac tcatcggata tggccacgct gaaaattatc ggcgacgact
     4621 aacgccagaa tatagaaacc acaaaaaatg aatgttaatt acctgaatga ttcagatctg
     4681 gattttcttc agcattgtag tgaggaacag ttggcaaatt tcgcccgatt gctcacccat
     4741 aatgaaaaag gcaaaactcg cctctccagc gtactgatgc gcaacgaact gtttaaatcg
     4801 atggaagggc atcccgagca acatcgccgc aactggcagc tgattgctgg agaattacag
     4861 cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc
     4921 attttgctcg atgtttcaaa acgattgaag ctgaaagccg acaaagagat gtctacgttt
     4981 gagattgagc aacagttact ggaacaattt ctgcgtaaca cctggaagaa tatggacgag
     5041 gaacataagc aggagtttct gcacgcggtc gatgccaggg tgaatgagct ggaagagctg
     5101 ctgccgctgc tgatgaaaga caaattattg gcaaaaggcg tgtcgcattt gctttccagc
     5161 caattgaccc gcattttacg cacccacgca gcaatgagcg tgcttgggca tggtttgctg
     5221 cgcggcgcgg gtctgggagg ccctgtgggc gcagcattaa atggggttaa agcggtcagc
     5281 ggcagtgcct atcgcgtgac aattccagcc gtaatacaaa tcgcctgcct gcgccggatg
     5341 gttagcgcca ctcaggtctg acaccgtaaa ccggggaaaa ttttcatttt cccctcaaaa
     5401 gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac actactgtta
     5461 ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta tgggcaacac
     5521 taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca ccattctgct
     5581 taacctgcac aacgtgggtt atttcgctct ggacggtatt attcttgcca tgggcatttt
     5641 ctacggcggc atcgcgcaaa tttttgccgg tctgctggag tacaaaaaag gcaacacttt
     5701 cggtttaacc gcattcacct cttacggttc tttctggttg acgctggttg cgattctgct
     5761 gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg tctacctggg
     5821 tctgtggggc gtatttacgc tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct
     5881 gcaattcgtt ttctttagcc tgactgtgct gtttgccctg ctggcgatcg gtaacattgc
     5941 cggtaacgcc gcaatcatcc actttgccgg ctggattggt ctgatctgtg gtgccagtgc
     6001 aatctatctg gcgatgggtg aagtactgaa cgagcagttt ggtcgcaccg ttctgccgat
     6061 tggtgaatcc cactaattgt tttgagatcc ccccgctcgg ggggattttt ttattcgcta
     6121 acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca cttctggtgc cgtttcaacg
     6181 tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc gtgtaccaca
     6241 acgttacccg cagcgtcctt cacaccttcc agcgtctctt taatagactt cggctgaccg
     6301 ggtaagttaa ggatcagcgc ctgtttgcga atcacaccaa cctgacgcga aaggatcgca
     6361 gttggtacaa aatgcaggct tatctggcgc atctgttcac caaagccagg catctcgcgg
     6421 tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc agttccgccc
     6481 gtggtgagca ccagatggca actcatttca tccactagct cacacaacgt ttgctcgatg
     6541 atcgcctgct catcggggat taagcgggtt tccagttcaa acggcgtggt tagcgccgat
     6601 gtcagccatt cttccagcgc agggatgcct ttatcctgat aaacgccgct ggatgcgcga
     6661 tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc gtttaaacag
     6721 tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggcttca gaagaggtag
     6781 cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat cattttttcc
     6841 agtttttcct ggtcaatagc aaacttacgg ataccttccg ccagtttatc tactgccatt
     6901 ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg acgcgctttc
     6961 acttcgccgg tgtaagacag tttacgttcg atagcccctt cgctctcagc cagctctttc
     7021 agcagtgccg gtgcgatggt aagacggtcg cagcctgcca gttccagaat ttcgccgatg
     7081 ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta gtactggtag
     7141 atttcagata cagaaaccac gcccggatct tctgccggag cgtactcttt cttatcagta
     7201 ttcgctttgt accagtcaag aatacggcca acaaatggcg agatcaggaa cacgcctgct
     7261 tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca gttgatgcct
     7321 tctttttcca gctgttctgc agcacggata ccctgccaag tagaagccag tttgatcaga
     7381 atacgatcgt tgctaatacc agcatcgttg tagagtttga tcaggcgttt tgctttcgca
     7441 attgacgctt cggtgtcata ggaaagacgc gcatcaactt cagttgagat acggcccgga
     7501 accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc gtccacgatc
     7561 tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc aatcaactta
     7621 cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt ggcatcctgc
     7681 ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga
     7741 cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct tgttaggggg
     7801 atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac
     7861 ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc gcccgccatt
     7921 tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta gcgtatatac
     7981 ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa taagagggat
     8041 atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg taatgattta
     8101 cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc agtttcgcta
     8161 catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag gcggtttaac
     8221 ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg
     8281 cgttgcgctg gctattaccg ccggtggacc tggagccgtc ttttggatgt gggttgccgc
     8341 gtttatcggc atggcgacct cgtttgccga atgttccctt gcacaacttt ataaagaacg
     8401 tgacgtcaat ggacagtttc gtggaggacc ggcatggtat atggcgcgcg ggctggggat
     8461 gcgttggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca taattttcag
     8521 cggagttcag gcgaacgccg ttgcccgagc cctgagtttt cttttgattt tcccccgctg
     8581 gtgacaggca ttattctcgc tgtctttgct ctgctggcaa tcactcgcgg tcttcatggc
     8641 gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt gctgaccagc
     8701 atggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc tatttttgag
     8761 agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt aagccaggcg
     8821 attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg ttcgacgcca
     8881 aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg gattgtccag
     8941 atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat gctgatatta
     9001 ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat ccagaaggcg
     9061 atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt tattctgttt
     9121 gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt ctttttacgc
     9181 ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc
     9241 ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat ggcctgcatg
     9301 gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac cattgccagt
     9361 gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg
     9421 gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga gtaatcacaa
     9481 ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct gcaaggactg
     9541 gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc gttgaccacc
     9601 acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca tgaagcgcgg
     9661 aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact ggcgggtatc
     9721 aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc ccgccaggct
     9781 attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt cagcgaagac
     9841 gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg cgtactgcgc
     9901 ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga gaatgcccga
     9961 gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa cgaggcgctc
    10021 gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt taaatcggtg
    10081 aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga gaagaacggc
    10141 aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt
    10201 attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga aggttacttc
    10261 tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga gcagcgttaa
    10321 gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcatgcaga
    10381 ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg catcaggcgc
    10441 tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga
    10501 ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg cggtccgtgt
    10561 gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag
    10621 tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa ttttactgac
    10681 gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac cagggaaagt
    10741 gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg
    10801 gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca atcctaatct
    10861 ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc tccataattt
    10921 cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga
    10981 ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgt
    11041 gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa cgtttcaccg
    11101 agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca
    11161 tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc gtgcggctcc
    11221 gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg
    11281 gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc
    11341 ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg
    11401 ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca
    11461 ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt caaatcgccg
    11521 aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg cagctgcgca
    11581 actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat cgagttagcc
    11641 gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt caccagcgcc
    11701 tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caacgtacag
    11761 aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac cactttcaca
    11821 ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga ggtcgcggtc
    11881 agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg
    11941 ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt
    12001 tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt
    12061 tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa
    12121 tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc atgcggaaaa
    12181 aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctcg
    12241 ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta
    12301 tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag
    12361 tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt cgggccggag
    12421 ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag
    12481 ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag cttcgcggca
    12541 agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg cccgtgcgca
    12601 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc cgtcgagact
    12661 ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
    12721 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
    12781 tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc cagcaaacgg
    12841 gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc gaccaccgaa
    12901 caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt catcgccact
    12961 ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac gatattttcc
    13021 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
    13081 gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt caccgccgcc
    13141 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
    13201 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccagcagc
    13261 ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
    13321 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattcccatc
    13381 cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa catagcgcaa
    13441 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
    13501 cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg gcagcacagg
    13561 ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga gaatcaatag
    13621 tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg tataacccat
    13681 ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
    13741 ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac ctgcattgag
    13801 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
    13861 tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca tcgacgaggt
    13921 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg
    13981 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
    14041 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg caagttcttc
    14101 ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg agttggcaac
    14161 gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac gcttcagttg
    14221 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
    14281 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
    14341 cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt tgatattggc
    14401 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
    14461 taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta agccttcttt
    14521 cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt cgctttgcgg
    14581 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
    14641 ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg
    14701 gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc
    14761 gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc cggtattttt
    14821 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
    14881 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca acaacctcgc
    14941 atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc aaatctcgca
    15001 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc
    15061 aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca gcaccatgtg
    15121 atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat cgacggtaga
    15181 ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg taacgttgtg
    15241 accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
    15301 cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat gcaggacatg
    15361 ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg ggaatcccgg
    15421 ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt
    15481 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
    15541 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
    15601 atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
    15661 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact gtcaggtgcg
    15721 ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat
    15781 ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat
    15841 ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt
    15901 taatttactc atggcaggta accagttcag aagctgctat cagacactct ttttttaatc
    15961 cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg
    16021 cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt
    16081 gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt ttatgtcatc
    16141 tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt gcagttgatg
    16201 ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca
    16261 atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc
    16321 agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa
    16381 caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac
    16441 tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc
    16501 gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa taacatcacc
    16561 agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat cgacaatccg
    16621 cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct
    16681 ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga ccaaataacc
    16741 gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat tcttaacgta
    16801 agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact
    16861 aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat aattttataa
    16921 aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc aactagttgg
    16981 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
    17041 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc
    17101 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
    17161 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
    17221 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
    17281 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
    17341 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
    17401 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
    17461 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
    17521 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
    17581 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
    17641 atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc
    17701 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
    17761 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
    17821 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
    17881 aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccacacg
    17941 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
    18001 cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt gatctggaag
    18061 attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc
    18121 aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg gctttcattt
    18181 tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg ccggccagcg
    18241 ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg agcagtcccc
    18301 acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg cgccagcctt
    18361 tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg gcgattatcc
    18421 atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa aacatcacca
    18481 atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa
    18541 aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt
    18601 ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg aggaagtttt
    18661 cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc
    18721 tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc aaaaaatcac
    18781 ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag
    18841 gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc ttcaggttct
    18901 gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc
    18961 cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc accacaccaa
    19021 taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg atatttggcg
    19081 cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc
    19141 agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg ccatcaacca
    19201 tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct
    19261 cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca acaagttttt
    19321 gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa tcggcacgtt
    19381 cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca
    19441 gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt gcaatggcag
    19501 caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa
    19561 gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac
    19621 gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg
    19681 gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata ccactaccgg
    19741 tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc gttatacgac
    19801 cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc tgatccagta
    19861 acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg atgttttttt
    19921 ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc acatcaacag
    19981 cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttccacca
    20041 gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc actacttcag
    20101 gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta
    20161 gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc
    20221 gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga tcgggaagag
    20281 gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca atggagcggt
    20341 tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa atggcgctgg
    20401 cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc
    20461 ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca
    20521 ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac tgaccacgca
    20581 acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta tttgccgaat
    20641 cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg cgcactttgt
    20701 taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgagcca
    20761 gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagcaacg
    20821 ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga
    20881 caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca
    20941 accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc gatgagtatt
    21001 aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt taatgtgggt
    21061 atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca ttagttgctg
    21121 gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg
    21181 cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa
    21241 atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac gcagtaaggt
    21301 gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc
    21361 aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg
    21421 taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga tatcaggcaa
    21481 accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa
    21541 gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc
    21601 ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg
    21661 ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac
    21721 aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac
    21781 gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc
    21841 tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatagc
    21901 cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg ttttcgcccg
    21961 gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt
    22021 gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc
    22081 caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt
    22141 accattttta tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag
    22201 aacaaaatct caatactttt atttccgtca agccctaaaa catactctac taagaaaaaa
    22261 acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg
    22321 ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc
    22381 tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt
    22441 tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg
    22501 tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg
    22561 attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc
    22621 aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt
    22681 ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc
    22741 ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc
    22801 tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg
    22861 accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac
    22921 gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg
    22981 acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg
    23041 ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc aatgctcaac
    23101 ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga cgccaaagcg
    23161 ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc
    23221 agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacgacccag
    23281 ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga
    23341 cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt
    23401 gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc
    23461 agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg
    23521 tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg
    23581 atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg
    23641 tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta
    23701 ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc
    23761 tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac
    23821 tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg
    23881 taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg accaccaatc
    23941 ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc
    24001 atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc agtgaggaaa
    24061 cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt
    24121 tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg gtggtgattg
    24181 aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct gcggtgattc
    24241 tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat accggtattg
    24301 atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg
    24361 ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc
    24421 aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg
    24481 atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag caacacctct
    24541 tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc
    24601 gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc gccacgcaac
    24661 agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc
    24721 aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa
    24781 cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc
    24841 ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg tactcgctaa
    24901 agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc
    24961 tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa gccagcacgt
    25021 tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc acagcgtcga
    25081 tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg ttataagctg
    25141 ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg taactggtgc
    25201 caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa ccaggaatag
    25261 agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg ccgctggccc
    25321 ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat
    25381 agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg taagccagcg
    25441 ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga acgctaagat
    25501 cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt tttgacttgc
    25561 tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag tacatcaact
    25621 cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc tgcggcgctt
    25681 tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc gggtagaaac
    25741 cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca
    25801 gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc atcggcagac
    25861 gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt cgcacacggc
    25921 gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat ttcgcctgct
    25981 cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct
    26041 gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt gcattttcag
    26101 catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta aaatcggtcg
    26161 ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg
    26221 ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggccag
    26281 tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga tcttgtttac
    26341 ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta
    26401 gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat
    26461 agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc
    26521 cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg
    26581 gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca
    26641 gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc ttcagtccag
    26701 gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg atttgcgcgt
    26761 aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac
    26821 agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct
    26881 ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc taaccagtca
    26941 ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt gatcgaccaa
    27001 ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca tcacgataaa
    27061 catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt
    27121 tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa
    27181 aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca ttatggcggg
    27241 cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttacct
    27301 gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg aagaagcggt
    27361 ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc
    27421 ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga tcattcaggc
    27481 tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc tacgtctgcc
    27541 ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tagcgttgtg
    27601 ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga
    27661 tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca ccgtggtggc
    27721 gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg aacttgaccg
    27781 cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga aagatcagcg
    27841 cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga
    27901 gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc tggcacgctt
    27961 tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac tgtttattcc
    28021 acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg
    28081 tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa tcgtcgggat
    28141 catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg gtcaggaaca
    28201 gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcgtcgg ttgatcagtt
    28261 ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat
    28321 tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa
    28381 aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg gtcgtctgca
    28441 tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga
    28501 cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc
    28561 gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca tcctggacta
    28621 ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg aagagtacaa
    28681 gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca agcgtattgc
    28741 gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta caaaccaaga
    28801 aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc gcatcaggca
    28861 ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac agccttagta
    28921 tccgcaattt cgtagcgggt cacccgtgca ggttttttca aaccttgttt aatctcgaca
    28981 gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc gcctttgagc
    29041 cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc acgcagcgca
    29101 ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca
    29161 gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg aggctgcgta
    29221 aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg aaacggtatt
    29281 catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg cagaacgcct
    29341 gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc actgcaaaat
    29401 caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc aatgcgatct
    29461 catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca ctggtggtat
    29521 tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg ccaccaagga
    29581 tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc cagtagcgga
    29641 acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc ggatcgacca
    29701 gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct tccgaggtgt
    29761 agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg ttgctccaca
    29821 catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga atgcgaatat
    29881 ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc gtgggctgct
    29941 gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa cccataatga
    30001 tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt ccggtatgca
    30061 gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga aattcaagcc
    30121 ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt ttggcattca
    30181 gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc tgtagcgtgc
    30241 agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct ccctccgtat
    30301 ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa cgccaaaaat
    30361 ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa
    30421 accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc ggatctcccc
    30481 tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt cacgcaggct
    30541 ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct tcacgccgcc
    30601 gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac ggtgcaagac
    30661 agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc gcggattcgc
    30721 catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg ttccttccca
    30781 caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga aaattgccga
    30841 agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca gctcattcac
    30901 cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg catcaccatc
    30961 cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca gcgagccatc
    31021 tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag
    31081 ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta catccgccat
    31141 atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt cggcaatcag
    31201 gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg gcaggccaag
    31261 gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat acagcgtttt
    31321 catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc ccgcacctgg
    31381 gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta cgcggtcgaa
    31441 ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg agagtttctg
    31501 gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac gcgccaccgt
    31561 tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg cactgcactg
    31621 cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc gttttggagc
    31681 ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg cgagcaatta
    31741 cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag cgtgacggat
    31801 ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc ccagccctgc
    31861 tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct gcgccagcgg
    31921 gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg cgtctacgat
    31981 gtcgccaatc acattgccgg taaatttacc gtccatgatc tccagttcat tggctaccac
    32041 ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc cgccggaggc
    32101 aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta agcctggcat
    32161 cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc
    32221 cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc gttcggttac
    32281 ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac attcaatctg
    32341 gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt gcgggatttt
    32401 ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc gtgcggtgag
    32461 tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa caatcaccat
    32521 cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc cacgaccata
    32581 cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat caccacttaa
    32641 tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc aggtaatgtt
    32701 aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg aggctacctt
    32761 gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa gatggctcgc
    32821 acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct tgccttgtta
    32881 gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca gcgtaatcca
    32941 cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt tgcaccgctg
    33001 atgcgtaccg actcaccaga tgaaaaacgc attcaggcga tcctcgatca gttaacggat
    33061 gaaagccgca tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat cgcccgttca
    33121 ggcgaaagcg tcgaagtgcg cgaccggcta gcgctcgacg gtaaaaaagc aggcggctat
    33181 tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg ctatctgcgc
    33241 ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa caccactaac
    33301 attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac ccgcacgctg
    33361 ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg
    33421 gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc
    33481 gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt taacctggcg
    33541 gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt tctctggcgc
    33601 aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg
    33661 cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg cgcggtgttc
    33721 cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta cgataaaacc
    33781 atctctacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc cgaagcgtcc
    33841 ggacgtaacg atctggtggt gaaaacagtc gaaggcgacc gcaaagtctc aggctcggcc
    33901 tatcgcgaaa ccaaagatcg tggcttccac cacggcactt tgctgctcaa tgccgacctt
    33961 agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa aggcattacc
    34021 tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac ccatgagcag
    34081 gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt agaagcggaa
    34141 atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt tgcccgtcag
    34201 agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct ggatgaacgc
    34261 tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat cacccgcgcc
    34321 caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa
    34381 ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc
    34441 ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa
    34501 ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc aggagcggga
    34561 gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc gtatcaggca
    34621 taaattatca cttgctgaag taagtaaaat gttaggtatc tcgattaatt ctctaaaata
    34681 gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatgggag aaatttatgg
    34741 ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata aatgaataga
    34801 ttcgtgaata gatacttgaa tgtattttat tttttatc
//
LOCUS       DAZPJR010000026        33999 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPJR010000026 DAZPJR010000000
VERSION     DAZPJR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.027"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            12..514
                     /locus_tag="ACHVB7_001190"
                     /pseudo
     CDS             12..514
                     /locus_tag="ACHVB7_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHVB7_001191"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHVB7_001191"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR1913232.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHVB7_001192"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHVB7_001192"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR1913233.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHVB7_001193"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHVB7_001193"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR1913234.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHVB7_001194"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHVB7_001194"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR1913235.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHVB7_001195"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHVB7_001195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR1913236.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHVB7_001196"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHVB7_001196"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR1913237.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHVB7_001197"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHVB7_001197"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR1913238.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHVB7_001198"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHVB7_001198"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR1913239.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHVB7_001199"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHVB7_001199"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR1913240.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHVB7_001200"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHVB7_001200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR1913241.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHVB7_001201"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHVB7_001201"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR1913242.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHVB7_001202"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHVB7_001202"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR1913243.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHVB7_001203"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHVB7_001203"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR1913244.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="ACHVB7_001204"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="ACHVB7_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR1913245.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18087..19304
                     /gene="alaA"
                     /locus_tag="ACHVB7_001205"
     CDS             18087..19304
                     /gene="alaA"
                     /locus_tag="ACHVB7_001205"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR1913246.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19388..19987
                     /gene="yfbR"
                     /locus_tag="ACHVB7_001206"
     CDS             19388..19987
                     /gene="yfbR"
                     /locus_tag="ACHVB7_001206"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR1913247.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20046..21878)
                     /locus_tag="ACHVB7_001207"
     CDS             complement(20046..21878)
                     /locus_tag="ACHVB7_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR1913248.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="ACHVB7_001208"
                     /pseudo
     CDS             complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="ACHVB7_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22624..23118)
                     /locus_tag="ACHVB7_001209"
     CDS             complement(22624..23118)
                     /locus_tag="ACHVB7_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR1913249.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23201..23656)
                     /gene="yfbV"
                     /locus_tag="ACHVB7_001210"
     CDS             complement(23201..23656)
                     /gene="yfbV"
                     /locus_tag="ACHVB7_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR1913250.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23994..25196
                     /gene="ackA"
                     /locus_tag="ACHVB7_001211"
     CDS             23994..25196
                     /gene="ackA"
                     /locus_tag="ACHVB7_001211"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR1913251.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25271..27415
                     /gene="pta"
                     /locus_tag="ACHVB7_001212"
     CDS             25271..27415
                     /gene="pta"
                     /locus_tag="ACHVB7_001212"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1913252.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27605..29125
                     /gene="yfcC"
                     /locus_tag="ACHVB7_001213"
     CDS             27605..29125
                     /gene="yfcC"
                     /locus_tag="ACHVB7_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR1913253.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="ACHVB7_001214"
     CDS             complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="ACHVB7_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR1913254.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="ACHVB7_001215"
     CDS             complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="ACHVB7_001215"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR1913255.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="ACHVB7_001216"
     CDS             complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="ACHVB7_001216"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR1913256.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31145..31792
                     /gene="yfcG"
                     /locus_tag="ACHVB7_001217"
     CDS             31145..31792
                     /gene="yfcG"
                     /locus_tag="ACHVB7_001217"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR1913257.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31849..32211
                     /gene="folX"
                     /locus_tag="ACHVB7_001218"
     CDS             31849..32211
                     /gene="folX"
                     /locus_tag="ACHVB7_001218"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR1913258.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32232..33125
                     /locus_tag="ACHVB7_001219"
     CDS             32232..33125
                     /locus_tag="ACHVB7_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR1913259.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="ACHVB7_001220"
     CDS             complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="ACHVB7_001220"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR1913260.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8481 a         9279 c         8562 g         7677 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccaag cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctagtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatctgat gcaaagttat gttaggcaaa gtaatcttct attattaata
    17641 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
    17701 aattccgctt aacaatagtt tcacaccttg cccccattgc gacacctcgg aaatcatcac
    17761 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
    17821 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatc
    17881 agaacaattc actatataac attgcatgca aagcatatac gcctcattat tttgtcatta
    17941 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
    18001 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
    18061 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
    18121 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
    18181 gtaacaaggt actgaaactg aacatcggca acccagcccc attcggtttt gacgcgccag
    18241 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
    18301 aagggcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
    18361 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
    18421 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactct
    18481 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
    18541 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
    18601 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
    18661 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
    18721 aaattctcta cgacgatgct gagcatcact caattgcgcc actggcacct gacctgctga
    18781 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
    18841 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
    18901 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
    18961 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
    19021 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
    19081 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
    19141 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
    19201 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
    19261 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
    19321 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
    19381 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
    19441 ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
    19501 gatggtcgcc catgcgctgg cagctatcaa aaatcgaaaa tttggcggta atgtcaacgc
    19561 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
    19621 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
    19681 aatcgctcag caaaaactgg tcaatatggt tccggaagag ctgcgggata tctttgcgcc
    19741 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
    19801 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
    19861 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
    19921 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
    19981 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
    20041 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
    20101 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
    20161 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
    20221 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
    20281 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
    20341 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
    20401 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
    20461 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
    20521 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
    20581 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
    20641 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
    20701 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
    20761 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
    20821 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
    20881 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
    20941 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
    21001 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
    21061 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
    21121 tcacgtaatt tgagatcatc cagccgttga ccaatcattg gcgatccggg gcgaatagcc
    21181 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
    21241 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
    21301 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
    21361 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
    21421 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
    21481 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
    21541 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
    21601 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
    21661 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
    21721 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
    21781 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
    21841 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
    21901 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
    21961 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
    22021 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
    22081 tgacatgaca acccgccgcc aggccagaaa gcacgccagc ggagcatctt ccaccaccac
    22141 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
    22201 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
    22261 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
    22321 tgctttattc aaatgactga gtaaggcgat tgcccccgga agcgggtaat accttcggtt
    22381 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
    22441 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
    22501 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
    22561 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
    22621 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
    22681 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
    22741 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
    22801 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
    22861 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
    22921 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
    22981 ttcacgcatc tgtaatccgt aacctcgctc aataattgtt tgcaggcgac ggtaacgttc
    23041 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
    23101 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
    23161 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
    23221 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
    23281 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
    23341 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
    23401 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agctgaccgc
    23461 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
    23521 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
    23581 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
    23641 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
    23701 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
    23761 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
    23821 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
    23881 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
    23941 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
    24001 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
    24061 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cctgaagcac
    24121 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
    24181 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
    24241 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
    24301 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
    24361 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
    24421 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
    24481 tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
    24541 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
    24601 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
    24661 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
    24721 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
    24781 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
    24841 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
    24901 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
    24961 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
    25021 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
    25081 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
    25141 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
    25201 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
    25261 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
    25321 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
    25381 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
    25441 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
    25501 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
    25561 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
    25621 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
    25681 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
    25741 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
    25801 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
    25861 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc ctctgccggt
    25921 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
    25981 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
    26041 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
    26101 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
    26161 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
    26221 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
    26281 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
    26341 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
    26401 gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac
    26461 tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac
    26521 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
    26581 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga
    26641 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa
    26701 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
    26761 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
    26821 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
    26881 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
    26941 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
    27001 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
    27061 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
    27121 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
    27181 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct tcccggatct
    27241 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
    27301 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
    27361 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaacctcg
    27421 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
    27481 ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
    27541 atgaattgcg ccatttcact ttacatactt accactttgt tttgtgcaag ggaatatttg
    27601 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    27661 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
    27721 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    27781 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    27841 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    27901 attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat
    27961 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    28021 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    28081 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    28141 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
    28201 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
    28261 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    28321 cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    28381 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    28441 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    28501 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    28561 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    28621 taataccatg gcttcatcct ttaccgaagg gacgcgaatg atgatcgccc ctgccctgct
    28681 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    28741 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    28801 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
    28861 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    28921 ccgtcaggtt accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc
    28981 aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct
    29041 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    29101 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    29161 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    29221 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    29281 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    29341 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    29401 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    29461 ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    29521 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    29581 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    29641 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    29701 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    29761 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    29821 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    29881 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    29941 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    30001 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    30061 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
    30121 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    30181 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
    30241 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    30301 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    30361 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    30421 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    30481 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    30541 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    30601 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    30661 ggatgggcat cagatcgctg cgcagccagg cctgaatccg tcgtgcacgc gcacgatttt
    30721 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    30781 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    30841 gtgtctgacc gtaaccttgc cacgtcggct tcaaatgttc accgctgtcg aggtcgatgg
    30901 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    30961 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    31021 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    31081 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    31141 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    31201 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    31261 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    31321 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    31381 gactgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    31441 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    31501 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    31561 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    31621 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    31681 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    31741 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    31801 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    31861 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    31921 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    31981 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    32041 attcagcatg tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc
    32101 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    32161 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    32221 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    32281 ttccgcgttt gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag
    32341 ccagttccgt tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc
    32401 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    32461 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    32521 atttgattaa tgccagcgac acgccgccgt cggtattcat ttccggctcg gcaacgggct
    32581 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    32641 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    32701 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    32761 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    32821 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgacctgc
    32881 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    32941 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    33001 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    33061 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    33121 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    33181 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    33241 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    33301 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    33361 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    33421 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    33481 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    33541 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    33601 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    33661 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    33721 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    33781 tgcgaaaagc cattaattct tccagcttac tttggtgctc tatcactaca taaatataac
    33841 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    33901 catcaataaa actgtttgat tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    33961 gcgcgggaag atgaatatca ataaaatccc gcgcggtgt
//
LOCUS       DAZPJR010000027        32837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPJR010000027 DAZPJR010000000
VERSION     DAZPJR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.028"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHVB7_001221"
     CDS             267..1925
                     /locus_tag="ACHVB7_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR1913261.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHVB7_001222"
     CDS             complement(1922..2878)
                     /locus_tag="ACHVB7_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR1913262.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHVB7_001223"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHVB7_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR1913263.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHVB7_001224"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHVB7_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR1913264.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHVB7_001225"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHVB7_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR1913265.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHVB7_001226"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHVB7_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR1913266.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHVB7_001227"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHVB7_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR1913267.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHVB7_001228"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHVB7_001228"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR1913268.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHVB7_001229"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHVB7_001229"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHVB7_001230"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHVB7_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR1913269.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHVB7_001231"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHVB7_001231"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR1913270.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHVB7_001232"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHVB7_001232"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR1913271.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHVB7_001233"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHVB7_001233"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR1913272.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHVB7_001234"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHVB7_001234"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR1913273.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="ACHVB7_001235"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="ACHVB7_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR1913274.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHVB7_001236"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHVB7_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR1913275.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="ACHVB7_001237"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="ACHVB7_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR1913276.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="ACHVB7_001238"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="ACHVB7_001238"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR1913277.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="ACHVB7_001239"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="ACHVB7_001239"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR1913278.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHVB7_001240"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHVB7_001240"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR1913279.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHVB7_001241"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHVB7_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR1913280.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHVB7_001242"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHVB7_001242"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR1913281.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHVB7_001243"
     CDS             complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHVB7_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR1913282.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="ACHVB7_001244"
     CDS             29803..30951
                     /locus_tag="ACHVB7_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1913283.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="ACHVB7_001245"
                     /pseudo
     CDS             31161..32591
                     /locus_tag="ACHVB7_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            complement(32592..>32837)
                     /locus_tag="ACHVB7_001246"
     CDS             complement(32592..>32837)
                     /locus_tag="ACHVB7_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1913284.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8057 a         8030 c         8813 g         7937 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcac
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtacgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcttc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggct tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctactgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccctaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatc
//
LOCUS       DAZPJR010000028        32602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPJR010000028 DAZPJR010000000
VERSION     DAZPJR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.029"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            120..262
                     /locus_tag="ACHVB7_001247"
                     /pseudo
     CDS             120..262
                     /locus_tag="ACHVB7_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            424..744
                     /gene="cyaY"
                     /locus_tag="ACHVB7_001248"
     CDS             424..744
                     /gene="cyaY"
                     /locus_tag="ACHVB7_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR1913285.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="ACHVB7_001249"
     CDS             complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="ACHVB7_001249"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR1913286.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            3717..4658
                     /gene="hemC"
                     /locus_tag="ACHVB7_001250"
     CDS             3717..4658
                     /gene="hemC"
                     /locus_tag="ACHVB7_001250"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR1913287.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4655..5395
                     /gene="hemD"
                     /locus_tag="ACHVB7_001251"
     CDS             4655..5395
                     /gene="hemD"
                     /locus_tag="ACHVB7_001251"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR1913288.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            5417..6628
                     /gene="hemX"
                     /locus_tag="ACHVB7_001252"
     CDS             5417..6628
                     /gene="hemX"
                     /locus_tag="ACHVB7_001252"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR1913289.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            6631..7827
                     /gene="hemY"
                     /locus_tag="ACHVB7_001253"
     CDS             6631..7827
                     /gene="hemY"
                     /locus_tag="ACHVB7_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR1913290.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            8489..8626
                     /locus_tag="ACHVB7_001254"
                     /pseudo
     CDS             8489..8626
                     /locus_tag="ACHVB7_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(8734..9969)
                     /locus_tag="ACHVB7_001255"
     CDS             complement(8734..9969)
                     /locus_tag="ACHVB7_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1913291.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(10116..10192)
                     /locus_tag="ACHVB7_001256"
     tRNA            complement(10116..10192)
                     /locus_tag="ACHVB7_001256"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10156..10158),aa:Pro,seq:tgg)
     gene            complement(10235..10321)
                     /locus_tag="ACHVB7_001257"
     tRNA            complement(10235..10321)
                     /locus_tag="ACHVB7_001257"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10285..10287),aa:Leu,seq:cag)
     gene            complement(10342..10417)
                     /locus_tag="ACHVB7_001258"
     tRNA            complement(10342..10417)
                     /locus_tag="ACHVB7_001258"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10382..10384),aa:His,seq:gtg)
     gene            complement(10476..10552)
                     /locus_tag="ACHVB7_001259"
     tRNA            complement(10476..10552)
                     /locus_tag="ACHVB7_001259"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10516..10518),aa:Arg,seq:ccg)
     gene            complement(10653..12038)
                     /locus_tag="ACHVB7_001260"
     CDS             complement(10653..12038)
                     /locus_tag="ACHVB7_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1913292.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(12229..12969)
                     /gene="wecG"
                     /locus_tag="ACHVB7_001261"
     CDS             complement(12229..12969)
                     /gene="wecG"
                     /locus_tag="ACHVB7_001261"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR1913293.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(12972..14324)
                     /gene="wzyE"
                     /locus_tag="ACHVB7_001262"
     CDS             complement(12972..14324)
                     /gene="wzyE"
                     /locus_tag="ACHVB7_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR1913294.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(14321..15400)
                     /gene="rffT"
                     /locus_tag="ACHVB7_001263"
                     /gene_synonym="wecF"
     CDS             complement(14321..15400)
                     /gene="rffT"
                     /locus_tag="ACHVB7_001263"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR1913295.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(15397..16647)
                     /gene="wzxE"
                     /locus_tag="ACHVB7_001264"
     CDS             complement(15397..16647)
                     /gene="wzxE"
                     /locus_tag="ACHVB7_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR1913296.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(16649..17779)
                     /gene="rffA"
                     /locus_tag="ACHVB7_001265"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(16649..17779)
                     /gene="rffA"
                     /locus_tag="ACHVB7_001265"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR1913297.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(17784..18458)
                     /gene="rffC"
                     /locus_tag="ACHVB7_001266"
                     /gene_synonym="wecD"
     CDS             complement(17784..18458)
                     /gene="rffC"
                     /locus_tag="ACHVB7_001266"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR1913298.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(18436..19317)
                     /gene="rfbA"
                     /locus_tag="ACHVB7_001267"
     CDS             complement(18436..19317)
                     /gene="rfbA"
                     /locus_tag="ACHVB7_001267"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1913299.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(19336..20403)
                     /gene="rffG"
                     /locus_tag="ACHVB7_001268"
     CDS             complement(19336..20403)
                     /gene="rffG"
                     /locus_tag="ACHVB7_001268"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1913300.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(20403..21665)
                     /gene="wecC"
                     /locus_tag="ACHVB7_001269"
     CDS             complement(20403..21665)
                     /gene="wecC"
                     /locus_tag="ACHVB7_001269"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR1913301.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(21662..22792)
                     /gene="wecB"
                     /locus_tag="ACHVB7_001270"
     CDS             complement(21662..22792)
                     /gene="wecB"
                     /locus_tag="ACHVB7_001270"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR1913302.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(22848..23894)
                     /gene="wzzE"
                     /locus_tag="ACHVB7_001271"
     CDS             complement(22848..23894)
                     /gene="wzzE"
                     /locus_tag="ACHVB7_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR1913303.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(23906..25009)
                     /gene="wecA"
                     /locus_tag="ACHVB7_001272"
     CDS             complement(23906..25009)
                     /gene="wecA"
                     /locus_tag="ACHVB7_001272"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR1913304.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(25249..26508)
                     /gene="rho"
                     /locus_tag="ACHVB7_001273"
     CDS             complement(25249..26508)
                     /gene="rho"
                     /locus_tag="ACHVB7_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR1913305.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(26593..26694)
                     /gene="rhoL"
                     /locus_tag="ACHVB7_001274"
     CDS             complement(26593..26694)
                     /gene="rhoL"
                     /locus_tag="ACHVB7_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR1913306.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(26835..27164)
                     /gene="trxA"
                     /locus_tag="ACHVB7_001275"
     CDS             complement(26835..27164)
                     /gene="trxA"
                     /locus_tag="ACHVB7_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR1913307.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            27295..28560
                     /gene="rhlB"
                     /locus_tag="ACHVB7_001276"
     CDS             27295..28560
                     /gene="rhlB"
                     /locus_tag="ACHVB7_001276"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR1913308.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            28694..30178
                     /gene="gppA"
                     /locus_tag="ACHVB7_001277"
     CDS             28694..30178
                     /gene="gppA"
                     /locus_tag="ACHVB7_001277"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR1913309.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(30225..32246)
                     /gene="rep"
                     /locus_tag="ACHVB7_001278"
     CDS             complement(30225..32246)
                     /gene="rep"
                     /locus_tag="ACHVB7_001278"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR1913310.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            32463..>32602
                     /locus_tag="ACHVB7_001279"
     CDS             32463..>32602
                     /locus_tag="ACHVB7_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913311.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         8225 a         8809 c         8180 g         7388 t
ORIGIN      
        1 tcatcgcagg agaagacgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt
       61 gaggaaatgc tgcgagtata acgcttactc gtggggattt atgttttgct ggtagtttcg
      121 tgatcggcac ctcgcgccag gatggctcta tgcactatcg caatgtggtt tgtttgttgt
      181 cttttttagt tccgtcagcg tgaggatgct cgctcggtaa acctgatcat aatgtttacc
      241 agaaacaggg aaaggcgtgg tgtaactgcg ccctaatgaa gagacaaatc tttcgctaca
      301 cagagacatc attattccgg tgacggatga atcgtgccgg aacgcagccg cagaggtgca
      361 aactttgaag ccctgacccc atgccgctat actgccgcca tcacttaaaa acaggataca
      421 acaatgaacg acagtgaatt tcatcgcctg gctgatcaac tctggctgac cattgaagaa
      481 cgcctggacg actgggatgg cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg
      541 accattacct ttgaaaacgg cagcaaaatc attatcaacc gccaggaacc gctgcaccag
      601 gtatggctgg caaccaaaca aggcggctac cattttgatc tgaaaggcga tgagtggatt
      661 tgcgatcgca gcggcgaaac cttctgggat ttgctggaac aggcggcgac gcagcaggcg
      721 ggtgaaacag tcagtttccg ctaagattgc atgccggata agcctcgctt tccggcacgt
      781 tcatcacgaa aaatattgct gtaatagcgg cgtatcgtga tcctgattgg caggcggcat
      841 gttaccgata gattttgtgc ggaacggaat cacctgttca cgaccatcaa ccttcacaat
      901 ctgatagaac tgcggcaggt tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg
      961 cgatgacgag tagaagcgac tgacgtcacg caccagttcc tctttgctgc cttcgcagtg
     1021 gtgatacacc tcaacccggt tgctttcgtc aagaatgtag atattaaagc cattctcgtc
     1081 ttgcgtttct tcgaagaaga actggatgat cccttcacta gcaaagccgt ccaccaccgc
     1141 cggtaatttg acgtgattgg tttcaacctg cactgacagg ccgtgcagtt tgttatgcga
     1201 aatagcgcca taaaactcga tggcgttctc cagcttctgt accgatacat tcaggcgttc
     1261 gaagaacaag ccccaggttt gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg
     1321 gcgggtgctg gaaaggcgca gttcaataca ctcagaaacc agttgctgca cgcgagtacg
     1381 gattaacccg cgcagatgct ggctataaca gaagacttcc acgctatctg gcggtgcggc
     1441 gtcctgatgc attttgccga gaatagtttt cagggcttcg atcatcgatt gctcgccgtt
     1501 gaagtgcagc gtacgcactt cgttccacga gttgcggtac agcaggtcaa cgctacctac
     1561 caggcaattt tgattctcgc caaagctgaa gacatccagc ttacggaaat caaaatgcac
     1621 cacctgattg cggaacgccg ctgtcgggtc atattccagg ttaacgataa tcgccagatg
     1681 gcggatctca cacgggctgt agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa
     1741 atggtgcgac acgtcggcga ccatctcctg caacttaggc aaatcgacaa tgccgttacc
     1801 tttaatatac aaacgggtgc gcgaggtcag cagaccgtta aaccatgccc acgccaccag
     1861 tttattcagg taacggttat attccagcgg ctgatggctg atgatcgact caatatttgg
     1921 cgcgcggtta tacagatacc aacctgaacg gttagcccgg cccggcggca cataaataaa
     1981 ggtcagattc ggttccgaga gatcgggtga aatctgcggg tttaccagcg tcactttacc
     2041 tggtaatgct tcaaacgcgg catacagctt acgcgtcagc acgccgatat cctgcggact
     2101 ggcggagacg ctaaggttat tgcgacgcgc aaagcggatc agattacggt agctctgcat
     2161 catcgcgtcg agcaactcgt tgtgcgcctc acgcacctga tcaatcttcc agttagcacg
     2221 gttatcgagc attgccagac gagcttcgtc ccaaccccac tcgctcacta actggctcaa
     2281 cactgcgcga cgccagccta cacaggcgcg ttcacggctg agtttttcgc acacttttaa
     2341 atagaagcag cgacgtacta aatccagacg ggtgaaatct tcaatcgccg tcaggtattc
     2401 agtaacacgc tccagcatca tgcagtatgg atcgaggcca aacgatacaa tctcgccgtc
     2461 gtgcaaacgc tgtttgatat ctttcgccag cagacgtggg ttcgggtatt cccaggaata
     2521 ggcttccagc agcagtgttt tcagcaccgc tttgtatggg gaatcgatac tcttgtagag
     2581 ctgccaaagg ctggcaccaa agtactcttc agcagaaagc gagcttaagc caccgagatc
     2641 cagccattca tttggcgtca gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta
     2701 atgctcttct tcgtcgcacg gcaccatatt ccacagaata cgcttaccgg cgagacgcac
     2761 ggcggtacga taaaattcgt caagcagcag tatatgctgg gtggagccac aatcttcgcc
     2821 ccccaggctg ccgctttcat tatgacgaaa gcggttttca tcaatcagga agaagctgac
     2881 ttccacaccc agcgaggcgg cccagctttc cagcaggctg catttacgtt gtagtaactg
     2941 gcgctcttcg ctatcgagcc aggattgatg acagacccag atatccaggt cagaggaaca
     3001 actttgccct acggacgagg tgctgcccat ggtgtataca ccagtaattg gaagctcacc
     3061 tttcggtgga tcctgtactg acattccacg atacagttca agctcgttca ggtagtggcg
     3121 ttgagtttca tcaggcgtgt aaaggcaaat gcctttggga acgttaccat caaggtaacc
     3181 cggcattagc ggatggtgat agtgcaacaa tgtcggcagt agactgtaga cctgttggaa
     3241 tgcaggcccc atagcagcaa gcgcgcgatc cacacgcaat tgatttatgg catccagtct
     3301 ctgtttcaga gtctcaatat agaggtacaa gacgtatcgc ctgatttgct acccgtcatg
     3361 actgtgattc cgccaacatc aacggtaaca cgcggcattc gggatatttc gtatgtcaaa
     3421 ggtaaccgtt accacttttc gcgcctggtt tttttagttt cacgacgaaa aaatggtcta
     3481 aaacgtgatc aatttaacac cttgctgatt gaccgtaaag aaagatgcgc tacatacaag
     3541 tgtagcaccg tttattctct gtaaattcct tattacgaca gcgtgaaacg tctgtcagga
     3601 tccactgcca gacctcattt tacggtttgc gcaggcgtct acgtttcacc acaacactga
     3661 catcactctg gcaaggatgt taggatggac cacggatgat aatgacggta acaagcatgt
     3721 tagacaatgt tttaagaatt gccacacgcc aaagcccact tgcactctgg caggcacact
     3781 atgtcaaaga caagttgatg gcgagccatc cgggcctggt cgttgaactg gtaccgatgg
     3841 tgacgcgcgg cgatgtgatt cttgatacgc cgctggcgaa agtaggcgga aaaggcttat
     3901 ttgtaaaaga gctggaagtc gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcaa
     3961 tgaaagatgt gccggttgaa ttcccgcaag gtctgggact ggtcactatt tgtgagcgtg
     4021 aagatcctcg cgatgccttt gtgtccaata actatgacag tctggatgcg ttaccagcag
     4081 gcagtatcgt cgggacgtcc agtttacgtc gccagtgcca actggctgaa cgccgcccgg
     4141 atctgattat ccgctccctg cgcggcaacg tcggcactcg cctgagcaaa ctagataacg
     4201 gcgaatacga tgccatcatt cttgccgtag ccggactaaa acgtttaggt ctggagtccc
     4261 gcattcgcgc cgcgttacca cccgagattt ctcttccggc ggtaggacaa ggtgcagtcg
     4321 gtattgaatg ccgccttgat gatacgcgca ctcgcgagct gcttgccgcg ctgaatcacc
     4381 acgaaactgc actgcgcgtt accgcagaac gcgccatgaa tacccgtctc gaaggcggat
     4441 gtcaggtgcc aattggtagc tacgccgagc ttattgatgg cgaaatctgg ctgcgtgcgc
     4501 tggtcggcgc gccggacggt tcgcagatta ttcgcggtga acgccgcggt gcgccgcaaa
     4561 atgccaaaca aatggggatt tcgctggcag aagagctact gaataacggc gcgcgcgaga
     4621 tcctcgctga agtctataac ggagacgccc cggcatgagt atccttgtca cccgcccgtc
     4681 tcccgctgga gaagagttag tgagccgtct gcgcacactg gggcaggtgg cctggcattt
     4741 tccgctgatt gagttttctc cgggtcaaca attaccgcaa cttgctgatc aactggcagc
     4801 gctgggggag agcgatctgt tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc
     4861 acagctacat cagcaagatc gtaaatggcc ccgactacct aattatttcg ccattggacg
     4921 caccaccgca ctggcactac ataccgtaag cggacagaag attctctacc cgcaggatcg
     4981 ggaaatcagc gaagtcttgc tacaattacc tgaattacaa aatattgcgg gcaaacgtgc
     5041 gctgatatta cgtggcaatg gcggtcgtga gctaattggg gataccctga cggcgcgcgg
     5101 tgctgaggtc actttttgtg aatgttatca acgatgcgca atccattacg atggtgcaga
     5161 agaagcgatg cgctggcaat cccgcgaggt gacgacggtc gttgttacca gcggtgaaat
     5221 gttgcagcaa ctctggtcgc tgatcccaca atggtatcgt gagcactggt tactacactg
     5281 tcgactattg gtcgtcagtg agcgtttggc gaaactcgcc cgggaactgg gctggcaaga
     5341 cattaaggtc gccgataacg ctgccaacga tgcgctttta cgggcattac aataactctc
     5401 ataacaggaa gccataatga cggaacaaga aaaaacctcc gccgtggttg aagagactag
     5461 ggaggccgtg gacaccacgt cacaacctgt cgcaacagaa aaaaagagta agaacaatac
     5521 cgcattgatt ctcagcgcgg cggctatcgc tattgctctg gcggcgggcg tcggtttgta
     5581 tggctggggt aaacaacagg ccgtcaatca gaccgccacc agcgatgccc tggctaacca
     5641 actgacggca ttgcaaaaag cccaggagag ccaaaaagcc gaactggaag gcattattaa
     5701 gcaacaggct acgcagcttg aacaggcgaa tcgtcagcaa gaaacgctgg caaaacagct
     5761 ggatgaagtc cagcaaaagg tcgccaccat ttccggcagc gatgctaaaa cctggctgct
     5821 ggcccaggcc gattttctgg tgaaactcgc cggacggaag ctgtggagcg atcaggacgt
     5881 cacgaccgct gcagcgttgc tgaaaagtgc agacgccagc ctggcggata tgaatgaccc
     5941 gagtctgatt accgttcgtc gggcaattac cgatgatatc gccagccttt ctgcagtatc
     6001 gcaggtggat tatgacggca tcatccttaa gcttaatcag ctttcaaatc aggtagataa
     6061 cctgcgtctg gccgataatg acagcgatgg ttcgccgatg gattcagacg gtgaagagct
     6121 ttccagttcc atcagcgaat ggcgtatcaa tctgcaaaaa agctggcaga actttatgga
     6181 caacttcatt acgattcgcc gtcgtgatga caccgccgta ccgctgttag cgccaaatca
     6241 ggatatctat ctgcgcgaaa atattcgctc tcgcctgctg gtcgcagcac aagctgtacc
     6301 gcgtcatcag gaagagactt atcgccaggc gctggagaac gtctccacct gggtacgtgc
     6361 ttactacgat actgatgatg ccaccaccaa agcgttcctc gacgaggtgg accagttaag
     6421 ccagcaaaat atctcgatgg atcttccgga aaccctgcaa agccaggcga tgctggaaaa
     6481 actgatgcag acccgcgtgc gtaacctgct ggcacaaccg gcagcgggga caacggaagc
     6541 taaacctgca cctgcacctg cacctgcacc tgcacctgca cctgcaccgc aaggtgatac
     6601 tccggcagcc gcgccgcaag gagaataacc atgctaaaag tgttattact ctttgttttg
     6661 ctgattgcgg ggatagtggt tggcccgatg attgccggtc atcagggtta cgtgctgatc
     6721 cagactgaca actacaatat cgaaaccagc gtcacgggcc tggcgattat tttgattctg
     6781 gcgatggtag tgctgtttgc cattgagtgg ctactgcggc ggatcttccg cacaggcgca
     6841 cacacccgtg ggtggtttgt cggacgtaag cgtcgccgtg cccgtaagca gaccgaacag
     6901 gcgctgctga aactggcgga aggcgattat cagcaagttg aaaagctgat ggcgaaaaat
     6961 gccgatcacg cggaacaacc ggtggtgaac tatctactgg ctgccgaagc cgcgcaacaa
     7021 cgtggtgatg aagcacgcgc caaccaacat ctggaacgcg cagcagagct ggccggcaat
     7081 gacaccattc cggtagaaat cacccgtgta cgtctgcaac tggcccgtaa tgaaaaccat
     7141 gctgcacgcc acggcgtgga taagctgctg gaagttacgc cacgccatcc ggaagtatta
     7201 cgtctggcgg aacaggcgta tatccgcaca ggtgcatgga gttctctgct ggatatcatc
     7261 ccatcaatgg cgaaagccca tgttggtgat gaagaacatc gtgcaatgct ggaacaacag
     7321 gcatggattg gcctgatgga tcaggcgcgt gccgataacg gtagtgaagg tttgcgtaac
     7381 tggtggaaaa accaaagccg gaaaacgcgt catcaggtag cgttgcaggt ggcaatggcg
     7441 gaacatctta ttgaatgtga cgatcatgat actgcccagc aaattatcat cgatggcctg
     7501 aaacgccagt acgacgatcg cctactgctg ccgattcctc gactgaaaac caacaatccg
     7561 gaacagctgg aaaaagtgct gcgccagcag atcaaaaacg tcggcgatcg cccgctgttg
     7621 tggagcacac tgggccagtc actgatgaag cacggagaat ggcaggaagc atcgctcgcc
     7681 ttccgcgcag cgctgaaaca acgtccggac gcctacgatt acgcatggct tgccgacgcg
     7741 ctggacagac tgcacaagcc ggaagaagct gcagcgatgc gtcgcgacgg tttgatgtta
     7801 acgttgcaga acaacccacc acagtagttc cttctcaccc ggaggcaagc acctccggga
     7861 cctttctgat gcataaaaaa acgcctgctc ttattacgga gcaggcgtta aaacaggtct
     7921 gtatgacaac agtgggtgct tcactcaacg ttgtgtccat ggtgtctgat gaggcataag
     7981 cgacatctgt cagtggacga taagcaccgt aaacggctct gcgtcattcc ggagtttatg
     8041 aggcactaag gcgaacataa gagatggaat gagcatctac ccgtttatta tgccacagag
     8101 aatcgggaaa taacatccct taacacttgt tatgagataa ttctgtaatc ctctttgctt
     8161 cctgagtgaa tatttaacct gagcttgatc ctacacatac ttattatgaa tgataaaatt
     8221 cattcaatta ataacacata tactaattgc cgttaaaact aaaaaagcat taataatcaa
     8281 cgcaatataa taaacccgcc ttacatatca actgcgccag aggtaggatt gaaaacgctc
     8341 tcctgatttt ccaattcatt ttctggataa ataaataatt tatttttgtc actattattt
     8401 atgtaatcat cctgtcaggg agagggatct caattatcaa tgcttaatta cgtcatcatt
     8461 ttgatgacat gaaaccggag cattttgcat ggaattttcg ttttcaccca aacttcttgt
     8521 tgttgctgtc gccgccgctc ttcctctcat ggccagcgca gcagataccc cgtcaactgc
     8581 caccgcacgc aaaggctttg ccggatacga tcacccaaac cagtatccac agaacgctgc
     8641 aatttattga atttgcacga tcatgtaggc cggataaggc gtttacgccg catccggcaa
     8701 tcaaccgcag gcggccacaa aatggccgct tttttactta ttcaccacca gcaaatgtgc
     8761 ctgcataatg tcactggccg ggcgaccgtg cgccagcaaa tcagccattg ctttaagata
     8821 tggcggtaga tggcggaaat aacgctgata cccggcacac aaataattca gccccggttt
     8881 gccgctggca tcgagcatga agcggtgttt cgggcagcct ccccagcacg cttttaacac
     8941 gttacaactg cgacactgcg ccggtaattg cttaaattta tcttcaccaa acacctgctg
     9001 ttgcggggaa tcgatcattt ctgcaattgt ttgctgatgc atattcccca gccgatattg
     9061 cggataaaca tagtgatcgc aggcgtaaac gtcgccgttg tgctcaacaa tcaccgagcg
     9121 cccacaggtt ggctgatgat ggcaaaccgc acccggcgca ccgacaaaat tggcaaacgc
     9181 ccattcgata ttcatcacga aaatcttgcc gacgtcgcgt ttgatccagt ggtcgaatat
     9241 cgccaccaga aattcaccaa attcatcggg gcgcaccgac cattccgtca gttccccctg
     9301 aatatcacca ggcgcatgca atttcagtcc atcgcgggca gctgtttcat cagccagacg
     9361 ctcgaccacc ggaataaact ggatgaattc gactcccgca tcgcacaaaa aatcataaac
     9421 ctgcaacggt tgctgcgcgc tggtgcggtt gacgcagacc agcacgttat agtcgacatg
     9481 atgtttttgc agcagcgtca gggcacgcat caccagctta tgcgttggtc tgccaccttt
     9541 ggtcacgcga tattgattgt ggatctcagc cggaccgtcc agcgataacc caacaagaaa
     9601 atgattttct gccagaaatg cacaccattt atcatcgagc agcacgccgt tagtctggaa
     9661 gctgttactt atcttcctgc cagcaccgta tttcgcctgt aactttacgg cacggcggta
     9721 aaaatccagc ccgagtaacg ttggttcgcc gccctgccag gtaaaagcca cttcgttttg
     9781 cggttcgctg gcagcgatat agtgacggac atacgcttcc agcgtgtcat cgtccatatg
     9841 cgtgactggc ttttcgcggt aaagggattg tttttcgaga taaaaacagt agtcacagtt
     9901 cagattacaa tcggaaccac tcggtttcgc catcacatga aaagcacgcg ttggaacctg
     9961 ttgcagcatg gtcactcctt gcctgaagtc atccttcaat acgttaaggg cgaaatcata
    10021 ccgtggggca atgagttatt acgggcgttt attgcgaagg gagggtgacg aaatgcacag
    10081 aaaacaaaaa accccgccga agcggggttc aaaattggtc ggcgagagag gattcgaacc
    10141 tccgacccac tggtcccaaa ccagttgcgc taccaagctg cgctactcgc cgaaatactg
    10201 ctttttgaat ttttagttca attctttaaa gtcgtggtgc gagggggggg acttgaaccc
    10261 ccacgtccgt aaggacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg
    10321 cattgtcaca acttctaata atggggtggc taatgggatt cgaacccacg acaactggaa
    10381 tcacaatcca gggctctacc aactgagcta tagccaccac tacaaatctt gttacgcggt
    10441 attactacca ccgcagctca agcgccggga ctaaatggcg cgcccgacag gattcgaacc
    10501 tgagacctct gcctccggag ggcagcgctc tatccagctg agctacgggc gcttagcgcc
    10561 gttgcggggg gataatacgg acttcccacc cctctgtcta gtcccttttt aaataaattg
    10621 cgcgtttggt tacggtttgt gcgttttgct gcttattcct ccagtttatg cgctttcccg
    10681 tggcgattaa ggccaaaaac tttataaatc gccgtcaccg ccagcataaa gatgatgcca
    10741 acaaacagcg acatacgcgt atcttcatta aagtacatgc cgatcaaaac gcaaatcagg
    10801 aatgccattg ttacgtagtt ggcccacggg aacaggattg agcggaacgg atggctggca
    10861 atcgccgctt tatgtgcacg ccgaaaacgc agctggctta tcaatatcac aaaccatggc
    10921 accatccccg gaagcacgct ggcactgtag acgtagacaa acacacgctg cggattggga
    10981 atgatgtagt tcaggcatga gccaattagc agaatagcaa tagatactgc cacacccgca
    11041 actggtacgc cgtgacggga aactttcgcc atcgccgccg gtaactgacg gtttttcgcc
    11101 agtgcgtaga gcatacgtcc gcaactgtac atgccgctgt tacagccaga gagcgcagcc
    11161 gtcagcacca caaagttgat aatgcccgcc gctgcggtaa taccgatttt ggcaaaagtc
    11221 agtacgaacg ggctgccgtt gctgccaatt tcattccacg ggaagatggt gacgataacg
    11281 aaaatcgcgc ctacgtagaa aatcaggatc cgccacagca ctttgcctac agcactgcgc
    11341 agcgtcacct gcggattctt cgcttcaccg gcagtaatgc caatcagctc cacgccctgg
    11401 taggacgcca ccacaataca cagcgcggtc aggaaccctt tccagccacc cgcaaagaaa
    11461 ccgccatgct ctgtgagatt gctaaaacca atcgactgcc cgccattgcc aaagccaaag
    11521 aaaatcacgc ccaggccaat gacaatcatc acgataatcg tggtgacttt gatcatcgcg
    11581 aaccagaact cgatttcgcc gtacaaccgc accgccgcca gattcgccaa cgccaccagc
    11641 gccaccgcga tcaatgcagg tatccactgc gccatctccg ggaaccagaa ctggacataa
    11701 acgccaatgg cggtgatttc tgagatcccc accgccatcc acataaacca gtaagaccag
    11761 gcggtgagat aaccaaaaaa cgggctcata taacgatgcg cataaacggc gaacgaaccg
    11821 gtaacaggtt caaggaacaa catttcgccc attgaacgca tgatgaaaaa gacgaacagc
    11881 ccggcgatga tataggccaa caatacggat ggcccggccc atttcagggt actggcggcc
    11941 cccataaaca ggccgacgcc aatagtgccc cccagggcga tgagttcgat atgtcgagct
    12001 tccagcccac gctgtagctc tggtttgtta tctgccataa atcctcgtgt tgtgtttgca
    12061 tgctttccgg tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa tgttttcgta
    12121 attcaggaga aatggcaaat aaagcattaa aaatttgaat gctttgtgta ataaaaaagc
    12181 agacaggcga cggagtgacc actccgtcgc tttacaaaga gaggaaaatc agaggttgcc
    12241 ggtgtagtgc cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc ggctcggctg
    12301 cgaaagcagg cggtagagcc actccagtcc cagcgtttgc cagattttcg gtgcgcgttt
    12361 tacgtgaccg gtgaaaacat cgtaagtccc gccaacgccc atatacagcg catctggatg
    12421 caccagacgg cagtcgcgca tgaagatctc ctgctttggc gatcccatcg caacggtgac
    12481 gatttgcgct ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct cgggtttaaa
    12541 ataaccatcc tgactgccaa cgatattcac attccattgg ttgcgcagtt tagcttcagt
    12601 ttgcgccagc acttcaggtt taccgcccac aagaaatacc ggcgtccctt ctttgcctgc
    12661 gcgcgccatc agctcttccc agagatcggc accggcaacg cgggaaacct gcgcctgcgg
    12721 gtacttttta cgtactgaac gtacaacgct gatgccatcc gcatatttaa attcggcagc
    12781 gttaattaac tccctgacct cggcgttatc ttcaatggtc agcatttttt cagcattaat
    12841 ggcaaccagc gttccctgct taagctgccc gtcagcaaac agataatcga gggcgtgctg
    12901 catatcacgc caaccaatca actgtaagcc acgcagcgta taggtcggtg ccgtggtgtt
    12961 gttattcatt gttatccttc aacctgcgtc cggagcgatg attttgtacg tttatgaatg
    13021 agtccggcgc tttcaaaaag ccagtacagc agttttgcga tcatcagaca tgcgccgaag
    13081 accacgataa aaaagactac gcgtgagaca aacgaatcca gcccttcacg cgccagcacg
    13141 atcatattga agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt atagcgatta
    13201 ggctcgcggt tgcccagctc atacagccag tcgaaccatt tgatgatcag accaaccacg
    13261 atcgccccga gcgggatgaa caacgcgccg cccatcacca ccagtgagcc tataagcgta
    13321 ggcgagatcg ccagtccgga gtggttattc agcacttccc aggtaaagta gttggctgag
    13381 ttcagcacca tactcgggcg acccggccac agccaggaag ggataaagac atagaaatcg
    13441 cggacaattg gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa cagcaacgcc
    13501 agattctccc acggggagaa ggtgtcgcga gtgagataga gaaacgtata gaacgcttca
    13561 tcgccgctca cattcattcc atagcgtttt agcgccagcc agaacatgcc aacaatcccc
    13621 agcacgcccg ccgccgccag catccacaac gaaatccagc cgcgaataat gccaataaac
    13681 aggaagatag cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat catataagtc
    13741 agcaagccaa aggcgaccgt gctgacgagg aaaaacagcc acgctttgct gtcctggcgc
    13801 agaaagtaga ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt taacgccacg
    13861 ccggagactt cactggagaa gatctgactg taggagttaa gccggaacag caaaaagccg
    13921 ttgtgcatga agaagatgcc gacgcttacc agcgcgatac ccatcaggat cacccacgta
    13981 agattggtct ccacgcggtt catggtaaac agcggacggc gcggtacatc agcaacgcgt
    14041 ttgcgtaggc gggttttgta ggtgacatag taaaccgcgt agaagcagcc cgcagaaagc
    14101 aacgcctgca acaagatttc tggcggcgcg acaccaacat caaagcgaaa taccagcacg
    14161 ctggtcagcg ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa gaagacattg
    14221 aagttaaagc gcacacggcg aaactcaaac caggtcagcg tggcaataaa cagcgtgcag
    14281 agcagccaga ccacaaacag gccactgaat tgcagcagac tcatgcgacc tccccggcgg
    14341 caatagccaa cgcccgctgc cagccttgta gatagttagg gctaaagaag gcaatggtgt
    14401 ttttatccac cgacgccaac tggcgctgcg cttcacgcac aatatcctcg ttgagatcgt
    14461 cggtagtaaa cagcaccggc aaatgctgtt ccgtcatatc ctgccagaac ggattttccc
    14521 ggttaagcac acaaggaatg cccgcctgaa tcagtaagca aagcgtacca atgccctgct
    14581 ggcgggcaaa aataaagtaa ccaagatcgc actgacgaag tagcgccaga taggcgtcaa
    14641 attccagttt ttcgctcaaa atttgcagat tttcttcgct gaataactcc agccccgcct
    14701 gacgaacttc ctcaatgtac gcttcgttat taggcggata tcccatcggc accaccactt
    14761 ttaccgtatc gccaaattgc tgatgaacgg cgcgcaaggc agcaatatgg tcattgctgc
    14821 ggtcaccgga gttccccacc agaatggtca ttttcccttc acgttgccga tcattcgcca
    14881 tcgtattgag agaagggtcc atccgcgtcg ggaagtacag cagttcgccc cgcacctttg
    14941 gatgcgtttt ggcaaaaaag ctcaaatcac cgcgggtggc aaatacacag ccgactcgct
    15001 tttgcgccag gcgacgtagc gggtaaaaaa gcttatatct caagccactg gaaagctcat
    15061 acaggtctgc cccccagata tgccagaaaa actggctggg cttaatgcca ccactcagca
    15121 gagccagcca cagtgtggga ttgaactgac cgtggaaaaa aaatcgctgc tgacggttag
    15181 cttttgcttt cgcgatgacc gcttccgcca gcgatttttt cccagggaaa aattgcacag
    15241 aaagcgccgg acagctatca cttaagccgt cgtccttgcc aacaaccata aactcgcgcg
    15301 cgtgctcgct cgtcgcggcc agcgcgtcat tgaaaaaccg caaaacggtt cggttatggt
    15361 gagggatatc cgatcccagt acgtgaatca gtacagtcat gcccgcctac gccagagtaa
    15421 aaacacgcca caacaaagag aaaaatagac gatataagtt gccatatatg cctgcgccgc
    15481 gcccagtgca ccatgcgcag ggattagcca gtgggcaaat accatcaata aagtgaactg
    15541 gctgacttcc gccagaatat aaaaccgcag tgacgctttg gcgatcacca gataaccaaa
    15601 gacataagcg cccactttta acacatcacc cactaactgc caggcaaaga gatcgcgcat
    15661 agcggtaaat ttattcgaca acagcaacca gatagcaaaa tcacgcagca accagacggt
    15721 aaaactcgcc gccgccactg ccggtaagac gaatttcagc gatttaacca cttcccgggt
    15781 gatatcgcgc ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc tgaacgatgc
    15841 cgtaataaat tgcaggtagg catcggaaat actgctcacc ccttgccaga tccccacttc
    15901 atcccagcta tactgcgccg ccagcagttt acgcatcatg atgtaagcaa caggcaaggt
    15961 caccgacgta atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca gaccgttatc
    16021 ccagctgggt ttcagatagc ttaacgggat gacaccacgt ttgatcaaca tgatggcggc
    16081 aggaatcacc accagcgcgg gaatcagcgc cagacccagc aacgcccctt cataaccgcc
    16141 caaacggtaa ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa tcagcgataa
    16201 cgcattacct gcggcatcgc gaaagccttt catcagcgcc agtaacaggt tgccccaggc
    16261 gatccccatt tgcaccagcg ccactaaacg caccagcccc tgatagtcgg tattaccaaa
    16321 cagtccctgg ctgattggcg cagctgccag cacaaaaacc agcgccatca gcgtagagaa
    16381 accaagtacc atcgctgatg aagtgccgac cacgcggcgc agctgttgcg gattatcatg
    16441 gtactgggca acgtatttgg ttacaccgtt aaagatgcca gctccggcaa gcacgccgag
    16501 cacggtaatc aactggcgga aatttgccgc cagcccaagc cccgccggac caaatgacac
    16561 agccagcaac ttaccgacca gtaacccggc accaatcttg accagtgtac tggccgccgt
    16621 ccacaaggac gcttttgcca acgacatatc aggagaagta gttcaacaaa gtcgcaatta
    16681 ccgtacgctg attgacgggc gacaggttgt agaacagcgg caggcgcagc aggcgctcgc
    16741 tctctttggt ggtgtagcga tcttcaccgt ggaactcacc aaagcgttcc cccgcagggc
    16801 aaccgtgcag cggaatgtaa tggaacaccg ccatgatttc cgcttctttc agaaagttaa
    16861 tcaacgcgct ccggtcatca atatcccgca gtttaatgta gaacatatgc gcgttctgca
    16921 cgcagccatc gggaatcgac ggcagctcga tacgcccggc tttcgccaga ggcgctaacg
    16981 catcgtagta gttttgccac agcgccagac gttgctggtt gatacgatcc gctgcttcca
    17041 gttgcgccca caggtacgca gcttgcagat cggacatcaa atagctggag ccaatatcgc
    17101 gccaggtata tttatcgacc tgaccacgga agaactggct gcggttagtg cccttttcac
    17161 ggatgatctc ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg ccgccttcac
    17221 cacccgccgt atagtttttg gtttcatgga agctaaagca gccaatatga ccaatggttc
    17281 ccagtgcacg ccctttgtaa gtggacatca cgccctgagc ggcatcttct accacaaaca
    17341 gattatgctt tttcgccaac gccataatgg tgtccatttc gcaggccaca cccgcgtaat
    17401 ggaccggcac gataacgcgc gttttgtcgg tgatcgccgc ttcaatcagc gtttcgtcga
    17461 tgttcatggt gtccgggcga acatccacaa aaacgatttt tgcgccacgc agcacaaagg
    17521 cattggcggt ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca ggctggatat
    17581 cgagcagcag cgccgccatc tccagcgaag cggtgcagga cggcgtcagt aacactttgg
    17641 cgctgccaaa acgttgctcc agccactgct ggcagcgacg ggtaaaaccg ccatcgccac
    17701 acagtttgcc gctacccatt gccgactgca tatagtcgag ttcggttccc accaccggcg
    17761 gtgcgttaaa tggaatcatg tgatcacctg tataaccagt acgcggtgct ttctacattc
    17821 gcaccacttt gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt cgccacccgc
    17881 aaagttgttt taccgcgagc atacgcccag tttagcgccg tttgcatcag ctcagcacct
    17941 gcaccgcgtc cagccagcag gccaattcgc gcatctgtcg cattgagttc ccgtaaagag
    18001 acataaccgc gaataccgcc ggacgccgca cgtaaaatca gacattgatg atcaaaggtg
    18061 ccgcgcacgg cattttcaat ccactgtgca taaaagcgtc cgctggcgtc aggcgcatac
    18121 cacggcgcac gaaaacggct ttgcgcaaat gcggcgctgg ctaactgacg taatgcggga
    18181 atatcggtct cttgtgccac tacagcacca ctatcactga cattgttcac gggtagtgcc
    18241 aaatcaactt caccttctac cagggagaat cccagctgtt gcagggcatc cagttcaccc
    18301 gtatttgatg ccgcaatttt ggcctgcacc cgtgaccacg gcgctaacac gtctggcgtc
    18361 aggagctgtg cttcagacgt aatgcgcacg atggcgctgt taacaccaaa gaaggcgttt
    18421 tcccaggtta gtggctcaat actggcgcgg acgggcacga agtaactcca gcagatattg
    18481 gccgtagcca gttttcgcta atgaactggc agcacgcttc acaccctcgt catcgagcca
    18541 gccgttacgc caggcgatct cttccaggca ggcaatctta aagccctggc gtttttctac
    18601 tgtctgcaca aaggtactgg cttcaatcag gctgtcgtga gtgccagtgt ccagccaggc
    18661 aaatccgcgc ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca tctggttgat
    18721 ggaggtaatc tccagttcac cacgctccga cggcttcacc tgctttgcgt actccacgac
    18781 tttactgtcg tagaaataaa gcccggtcac cgcccagttt gacttcggct gttttggctt
    18841 ttcttccagc gagatagcgc ggaaattgtc gtcaaactcc accacgccaa agcgttccgg
    18901 gtccatcacc tgatagccaa ataccgtcgc cccttccgtg cgcgccgcaa catgacgcag
    18961 cttcggactg aagccctgac cgaagaagat gttatcgccc agcaccagac aagaaggttc
    19021 accgttgagg aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg ggctgggctg
    19081 ttcggcgtat tccagctgga taccgaactc actaccatcg cccagcaggc gctggaaata
    19141 acctttatct tccggcgtag tgatgatgag aatttcgcgg atatcggcca acatcagcac
    19201 cgacagcgga tagtaaatca ttggcttatc gtaaatcggc aacagttgct tcgatacgcc
    19261 gcgcgtaatc ggatgcaatc gggtgccgga accgcccgcc aggataatac ctttcatgtt
    19321 tgcctccgct gaaaattagc ctttcagacc taaacgctcg ccctgatagc tgccgtcctg
    19381 cacctgcttc caccagcttt cattagccag ataccactgc accgttttac gcattccact
    19441 ttcaaaggtt tcctgtggca gccagccaag ttcacgggca attttcgaag catcaatggc
    19501 ataacgcaga tcatgccccg gacggtcagc gacaaaggtg atcaagtcac gatagtgcac
    19561 cacgccgtgc ggcttgttcg gagccagttc ttccagcagc tcgcaaatgg tttccacaac
    19621 atcgagattc ttacgctcgt tgtgaccacc aatattatag gtttcaccga ctttcccggt
    19681 ggtcgccacg caatacagcg cgcgggcgtg atcttccaca tacagccagt cacggatttg
    19741 ctgcccgttg ccatataccg gcagcggttt acccgccagc gcgttgagga tcatcagcgg
    19801 gatcagtttt tccggaaagt ggtaaggacc gtagttattc gagcagttgg tgataagcgt
    19861 cggcagaccg taggtccgca gccaggcgcg caccagatgg tcgctgctgg cttttgacgc
    19921 ggaataaggg ctgctcggcg catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg
    19981 caggtcacca tatacttcgt cggtggagat atgatgaaaa cggaacgctg attttttatc
    20041 ttccgtcagc gtattccagt aagcccgcgc cgcttcaagc aatgtataag tcccgacaat
    20101 gttggtttca ataaacgctg ccgggccgtc aatagaacgg tcaacatggc tttcggctgc
    20161 cagatgcatg acacagtctg gctgatgctc agtgaatacg cgtgccagtt ctgcccgatc
    20221 gcagatatca actttctcaa aggcaaagcg ctcgctttgc gcgaccggtg ccagcgacat
    20281 caggtttccg gcgtaggtca gcttatcgac cactaccacc gcgtcgctcg tttcgttgat
    20341 gatataacgc accagcgccg agccaataaa cccggcacca cctgttatca gaatttttct
    20401 catcagcgcc agactccttt ggcatcgacg acatactgct gatggacatt gtcgccattg
    20461 ataactttaa actgactatg atcgaccagc atcaccagca cgtctgccgt tgccagcgcc
    20521 tcgtcaagct gcgccagagt acaaagcccg gtcagttttt tcggcaactg gtggatgtta
    20581 ggctcaacaa ccagagtttc gccgctatgc cactgggcga tcagttcagc gatttccatc
    20641 gccgggcttt cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt
    20701 ttcagttcac tggcgcgttt atcggtagcc gccaggcaat cagccaccgc cgctttcacc
    20761 tgatcgataa cccagaacgg tttgtgatcg ttcacttcgc gcgcggtacg gataagccgc
    20821 gcctgctggg ggttctgtgc cacgataaac cacggatcaa cagcaatgca gtgaccgccc
    20881 acgccagggc caggctgaag aatattaacg cgagggtgac gattcgccag gcgaatcagt
    20941 tcccagacgt taatcccctg atcggcacaa atcagcgaca attcattagc aaaagcgata
    21001 ttcacatcgc ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg cgagttagtg
    21061 acgacacact caccttcgag gaaaattttg tacagttcgc tggcgcgggt cgaacaaacc
    21121 ggcgtcatac caccaatcac gcgatcgttt ttaatcagct cgaccataac ctggcccggt
    21181 aacacgcgtt ccgggcagta agcaatgttg acgtccgcct gctcgcccac ctgctgtggg
    21241 aaagtgagat ccggacgcat ctctgctaac cattctgcca tcttctcggt tgaccccacc
    21301 ggcgaggtgg attcaaggat caccagcgcg ccttttttca gcactggcgc aatggagcga
    21361 gcagccgatt caacgtaggt catatctggc tcatgatcgc ctttaaacgg cgtgggtaca
    21421 gcaatcagcc aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct
    21481 acggcagttt ttactacgct cgccaaatca ggctcgacga tatggatttc gccacgattg
    21541 atggtatcaa ccgcgtgttg gttgatatcg acaccaatca cctgtttttg ccgtgaggca
    21601 aacgctgctg ctgtaggcag cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa
    21661 ctcatagtga tatccgatta ttttttaacg cttccagaat gcgagagcat gcctgaccat
    21721 caccatacgg gttatgggcg cggctcatag tttgatattc gttttcgtct tttaaaagac
    21781 gcgtcacttc ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc gtacccgccg
    21841 tcaccgcttc cggacgctca gtggtatcgc gcatcaccag caccggtttc cccagcgaag
    21901 gcgcttcttc ctgaatgccg cctgagtcgg tcaaaatcag ccaggcgtgg ttcatcagcc
    21961 agacaaacgg taaatactcc tggggatcga tcagaatgac atttttcaca tgccccagaa
    22021 tgcgattgac cggctctctg acgttcgggt tgagatgcac cggatagaca atctggatgt
    22081 cctggtgcgt ggtggcaatg tctgccaacg cgtggcagat ttcttcaaag ccacgaccga
    22141 aactttcacg cctgtgaccg gtcaccagaa tcatcttttt atcggggtcg ataaacgggt
    22201 aatttgccgc cagttctgaa cgcagcgtat cgctgctcat cacctggtca cgcacccata
    22261 acagtgcatc aataactgta ttaccagtaa tgaagattcg gctatccgca acgttttcac
    22321 gcagcaagtt ttgccgggaa gtttcggttg gagagaagtg atacatcgcc agatgcccgg
    22381 tcaatgtacg gttagcctct tccggccacg gcgaataaag atcgcccgtg cgcagaccag
    22441 cctcaacgtg accaacagga atacgctgat aaaacgccgc caggctggtt gccagcgtcg
    22501 tcgttgtgtc gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga ataggtttta
    22561 gcccttccag aatccgacag gttatctctg tcaggccctg tcctggctgc attatgttga
    22621 gatcgtagtc aggtacaatg gaaaagagtt tcagcacctg atcgagcatc tcccgatgct
    22681 gcgcagtgac gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg
    22741 gcgccatctt gatggcttcc gggcgcgtac caaatacagt cagtactttc acatcgattc
    22801 tcttcgaata agcggcgagc gcctttgcgc tcaccgcagc agtgttgcta tttcgagcaa
    22861 cggcgggtta atgcgacacc agccccgatc agccccccga caatgcccca cataatcatc
    22921 aggaaggcac gacgtgggct atcgcgtttt accggttctt ccggcgtacg caaatagcga
    22981 taggtctgaa aacgcggatc cagggttgga ccaacattca gggtgtttaa catggcccga
    23041 ttctgatcat agtcgagatc aaaggccgga ccgacggcct gtaaattttc cagtcgagcc
    23101 tggagcattg gacgcccaag caggaacatt tctgaatcag gtaattcctc ggcaggcaca
    23161 tctgtcgcac tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg ttcaatactg
    23221 ttcatccggc ggtcgtagat ggctttcgcc acctcttcct gacgcttcac ctgtgctttc
    23281 atctggatgg tacgcgccgc ccatgcgcct ttcagctcat cattcagatg gctggctgca
    23341 cgctggctgg caaaagcaac atactgacgt aacaggttat tagcgtcagg cgcggtttca
    23401 gcaataagct tcacgctgtc attgaccgcg cgggtaaagt ctccggggat aaactggatg
    23461 ttgttaatca tttcatccag caacgccgca tcggctttgc tgttgcccac catccgctgt
    23521 ttgtaatagt cggtttgcag ccagaactct ctgcgggtat cccacgaggc cagttgcata
    23581 acaaactctt tataggcttc gtccatgacc gatggttggt cggcagaagc catgtttgaa
    23641 cggacatcca ggttacgcaa aaattgctgc tgcgagtaat atccccccag catattcacc
    23701 gttggacgat cggtaatcgc cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc
    23761 agcgcgatta acgcaaatgc cagccccatg ccaataatcc atagcttccc agcccacaag
    23821 gtacgaaaca acccacgaat atccagttca ttttcagcgt cttcggccgg tttcccaggc
    23881 attggttgtg tcatcacatc ctcatttatt tggttaaatt ggggctgcca ccacgatttc
    23941 tacgcagtct gcgttttacg cgcttaataa agcgagcaac tttccaggca cgcttaatgc
    24001 aatatccata gaggaagaat gctagcaaaa agagcaccag catgacccac tccgggacaa
    24061 aatgagaata ttctgccagc acgccaatgg aagcgagcag tgccgcagca agggtaatca
    24121 gcacaaacgc ctgacgggaa gtaaacccgg cacgcatgat caaatggtga atatgctgac
    24181 ggtcaggaga gaatgggctc atgcctttac gcaggcgacg gtacataatc gccaccatat
    24241 ccattagcgg aatggcgatt atccacaaag cggtaaccgg gctgatggga tgggttttgc
    24301 cctgggtcgt ttcgagcagg atccagataa cggtaaaacc aatcagcgta ctgcccgcat
    24361 cacccataaa gactttgtag cggcgaccca ggataccaag gttaagcatg atgtatggca
    24421 ggatggcggc gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca
    24481 aaatcatacc gattgctgca aacgagacgc aggacaaccc gcccagcaag ccatcaatgc
    24541 catcaaccat gttgaacgca ttaatggccg cccagacggc aaatagcgtc aggaagtaac
    24601 caaacggtcc gagcaccatc tcccaggagc caaagatata acccaggcta ctgagataaa
    24661 gtttgccgaa caccatcata acaatgccaa cagcggcctg tatggtggca cggattttta
    24721 cgctgatatc aaaacggtca tccagcgcgc caatgaaaac aagcacaccg gcacaagcga
    24781 gatagagaga tgcatgcgga atatagtaat cgacaattcc gaacgtgaag caaatccctg
    24841 cgtaaaccga aatcccccca acgagaggta tcaatccctg gtgacgtttg cggaagtttg
    24901 gtttatccac taaaccgact ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg
    24961 tgaataaaaa aatactgatg agatcagtac tcactgtcag taaattcaca atgcatgctc
    25021 tcagagaaaa ttattagcag aagtataacc acgaagacct ttattcagaa gggaaagtcc
    25081 gttccgaacc caccagtcct gtgcaaaaat taaccatttg atatgcaaat tgcataacac
    25141 gccagcaaat tccaggaatt ttcctaacat cgccagcgcg gcattaagat tacagatata
    25201 aaagcaaaac gccacgtaaa cacgtggcgt ttttggcata agacaaattt atgagcgttt
    25261 catcatttcg aagaaatcgt cattggtctt ggtcatggcc agtttattaa tgaggaattc
    25321 cattgcatcg atttcgccca tcgggtgaat gattttgcgc aggatccaca ttttctgcag
    25381 ttcttcctga gtagtgagca gttcttcttt acgggtaccg gaacggttgt agtcgatagc
    25441 cgggaagacg cgtttttcag cgatcttacg agagaggtgc agttccatgt tgcctgtacc
    25501 tttaaactct tcgtagataa cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc
    25561 gataatagtc aggctgccgc cctcttccac gttacgcgcc gcaccaaaga agcgtttcgg
    25621 acgatgcagg gcgttggcat ccacaccacc ggtcaacact ttacctgacg ccggaacaac
    25681 ggtgttgtaa gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa cgtctttctt
    25741 gtgctcaacc aggcgtttgg ccttctcgat caccatttcc gcaacctgaa cgtggcgaga
    25801 tgcgggttcg tcaaaggtag aagcaacaac ttcacctttt accagacgct gcatctcggt
    25861 tacttcttcc ggacgttcgt cgatcagcag aaccatcagc acacaatccg ggtggttgta
    25921 agcaatgctc tgggcaatgt tctgcagcag catggtttta ccggctttcg gcggtgccac
    25981 aatcagacca cgctgaccac gaccgatagg tgatgccaga tccagtacgc gagcagttaa
    26041 atcttcagta gaaccgttac cacgttccat acgcagacga gaatttgcgt gcagcggggt
    26101 taagttctca aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt
    26161 aactttcagc agcgcaaaat agcgttcacc ttctttcggc gggcgaatct taccagagat
    26221 ggtatcacca gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt agatgtcatc
    26281 aggaccggcg aggtaggagc tgtctgcgga acggaggaaa ccaaatccat cctgcaatat
    26341 ctccagtacg ccatcaccaa agatatcttc gccactcttt gcgtgctgct tcaggatggc
    26401 aaaaataatg tcctgcttac gcatgcgagc caggttttcc agccccatat tttcgccgag
    26461 agtgatcagc tcagaaaccg gcgtattctt taattcggta agattcatag tggtgtgagt
    26521 tcttaaactt ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag gatcatccat
    26581 gcctgtttac ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga
    26641 actgaaacga caagacggat tgagtgacga gccagaaatc tgttcacttc gcatttaaga
    26701 taaaacggga agcgttggaa attacaagat tcaaacttaa taaggtatgt ttaatacgaa
    26761 gtcaacacta agttagcatg actcacgccg ggcgtccagt ttttagcgac ggggcacccg
    26821 aacatgaaat tcccttacgc caggttagcg tcgaggaact ctttcaactg acctttagac
    26881 agtgcgccca ctttggttgc cgccacttca ccgtttttga acagcagcag agtcgggata
    26941 ccacggatgc catatttcgg cgcagtgcca gggttttgat cgatgttcag ttttgcaacg
    27001 gtcagtttgc cctgatattc gtcagcgatt tcatccagaa tcggggcgat cattttgcac
    27061 ggaccgcacc actctgccca gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc
    27121 gtgtcaaaac tgtcgtcagt caggtgaata attttatcgc tcatatataa ctccacagga
    27181 ataagcctgg cgtgttggtt tcgttgttgg tgtaacatta accaactaaa ggttgacttt
    27241 atttcaccgg atacgctttc gtaaagcaat agtaagctga tattctacca cactatgagc
    27301 aaaacacatt taacagaaca gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa
    27361 gcccttgaaa aaaaagggtt tcataactgt acgcccattc aggcactggc ccttccgctg
    27421 acgctggcgg gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg
    27481 tttcttacgt caacgtttca ttatcttctc tctcatcctg cgattgccga tcgcaaggtg
    27541 aatcaaccgc gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca gatccatgcc
    27601 gacgcagaac cgctggcgga agctactggc ctgaagctgg gtctggctta cggtggtgat
    27661 ggctacgaca aacagctgaa agtgctggaa agcggcgttg acattctgat tggcaccacg
    27721 ggtcgtttaa ttgactacgc caagcagaac cacattaacc tcggtgccat tcaggtagtg
    27781 gttctggatg aagccgatcg catgtacgat ctgggcttta ttaaagatat ccgctggctg
    27841 ttccgccgta tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg
    27901 taccgggtac gtgaactggc gttcgagcag atgaacaatg ccgaatatat tgaagtggaa
    27961 ccggaacaga aaacgggcca ccgtattaaa gaagagcttt tctacccttc taacgaagaa
    28021 aaaatgcgtt tgctgcaaac gctgatcgaa gaagagtggc cagacagagc gattattttc
    28081 gccaatacca aacaccgttg tgaagagatc tggggccacc tggcggcaga tggtcatcgt
    28141 gtcggtttgt tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt
    28201 acccgtggcg atctggatat tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt
    28261 ccggcagtga cgcacgtctt taactacgat ttacccgatg actgtgaaga ttacgttcac
    28321 cgtattggtc gtacaggtcg cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa
    28381 gagtatgcat tgaatttgcc tgctattgaa acctatattg gtcactcaat tccggtaagc
    28441 aaatacaatc cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct cacgcgccca
    28501 cgtacaggca atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa
    28561 ataaaatgtc atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg
    28621 atgagcatac tgtagtaagt gattcaggtg aaagaatgca gccaacacag agacagattg
    28681 aaggatgaag agtatgggtt ccacctcgtc gctgtatgca gccattgatc tcggttcgaa
    28741 tagttttcat atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat
    28801 aaaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa aatgccctgt ctaatgaagc
    28861 aatggagcgc ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc
    28921 ctcgcaaatt cgcgttgtcg ctacggcgac attacgtctt gccgtcaatg cgggtgattt
    28981 tattgccaat gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg gtgaagagga
    29041 agcacgtctg atttatcagg gcgttgctca cacaactggc ggtgccgatc agcgcctggt
    29101 ggtggatata ggcggtgcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc
    29161 gttgttcagc ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa
    29221 tctggggcag gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt
    29281 tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca
    29341 ggcgttacag gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt
    29401 gcagcaattg aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg
    29461 gctgacgctg gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga tcgccatttt
    29521 taccgaactg aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt
    29581 ctacggcatg ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat
    29641 tcagcgccgc tttatgatcg atattgatca ggcgcagcgc gtagccaaag ttgcggctaa
    29701 cttcttcgat caggtggaaa aggaatggca tcttgaagca ataagccgcg atttgctcat
    29761 cagcgcctgt cagcttcatg aaatcggcct gagcgttgac tttaaacaag cgccgcaaca
    29821 cgctgcttat ctggtgcgta atctggatct tcccggtttt acccccgcac agaaaaaatt
    29881 gctggcgacg ctactgctca accagactaa tccggtcgat ctctcatcgc tgcatcagca
    29941 aaatgccgta ccgccgcgcg tcgcagaaca actctgccgt ttactccgcc tggccatcat
    30001 ttttgccagc cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga
    30061 actgttgacc ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat
    30121 tattgctcag gaaagccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg
    30181 agtaagtgcc ggatgcgatg ctgacgcatc ttttccggcc ttgattattt ccctcgtttt
    30241 gccgccatca tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg
    30301 gcgctgacca ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa
    30361 aagcggctcg gctccgggcg caccagctcg ccgtactgac ggcgttcttt acacagcgta
    30421 aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg
    30481 atattatctt catcgatgct gctctggtgc ggcaaaaacc cttcttccat accgaccatg
    30541 tagacataag gaaactccag cccttttgac gcgtgcagag tcatcagttg cacctgatcc
    30601 agctcttctt cactctcacc acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc
    30661 tgggtgagcg tcatcggctc atccagttcg ctgccttcca gcatctccgt catccagcta
    30721 aacagttggt tgacgttctt catgcgcatt tcggcggctt tcgggctggg cgatgtttcg
    30781 tacagccagg attcataatc catgccgtgg atcagatcgc gcaccgcggc aatcggctcc
    30841 cgctccgcca gacgctggat ttctgccaac cagtgagtaa agcgggtcaa tgcttcataa
    30901 ccacgtccgc taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta
    30961 ttgcgcgtca tcgcccactc acccagcttt ttcagcgtcg ccgggccaat ctctctcttc
    31021 ggcgtgttaa cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga
    31081 taagccagca agtccttgat ttcaggacga gagaaaaacg acgtaccacc agaaattttg
    31141 tacgggatgc ggttttgcat caggaatttt tcaaacactc gcgactgatg gttaccgcga
    31201 taaagaatag cgtaatcttt gtactgcgtt ttattgacga agtgatgcgc aatcagttca
    31261 ccagtgacgc gctcggcttc atgttcttcg ttattcgcgc ttaaaacttt aagctctgtg
    31321 ccgtaaccca gttcggagaa cagacgcttc tcaaagacgt gcgggttatt ggcgatcagg
    31381 atgttcgccg ctttcaaaat acgtccggaa gagcggtagt tctgctcaag cttaatcacc
    31441 ttcagcgccg gaaaatcctg actcagcagc accaggtttt gtggacgtgc gccgcgccag
    31501 gagtagatcg actggtcgtc atcgcccacc acggtaaagc gcgcgcggct gcccaccagc
    31561 agtttcacca gttcatactg gctggtgttg gtgtcctgat actcatccac cagcagatag
    31621 cgaattttgt tctgccagcg ctcgcggact tcttcattgc gttgcagcag caacgtcggc
    31681 aataaaatca gatcatcgaa gtcgagaaca ttgcacgctt tcaggtgtgc atcatacagc
    31741 ccataacaat gggcaaaaat acggtcccgc tcgccaatcg cactggctgc cgcctgggac
    31801 ggtgttttga gatcgttctt ccagttagag atggtcgaaa tcaactgttg caggagaact
    31861 ttgtcatctt caatcagccc ttcggtcaac tctttgagca acgccagctg atcggtatcg
    31921 tcaaacagcg aaaagttcgc cttcatcccc agcgccgcat actcacgctt gatgatatcc
    31981 agccccagcg tgtggaaggt tgagatcatc agcccacgcg cctctttgcg acccagcgtc
    32041 tgtccgacac gctctttcat ctcgcgcgcc gccttattgg taaaggtcac cgccgcgata
    32101 tgtcgcgcct gataaccgca accgcggatc agatgggcga ttttattggt gatcacacga
    32161 gttttaccgg aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct
    32221 tgttgttggc cggggtttag acgcataggt gtattgctca atcttcgaac ggggggagga
    32281 ttgtagcaga atcgcatttg aatgtgaccg cgtgtaagcc gctaaccgat tggctgcgga
    32341 tgtttaaatc agagagggaa gaccgaaacc ggagttcatc gtgaatccat tagacttaga
    32401 aaatatcggg tcgtaacgtt gtgttattac tttttcagag tttactaagg agaggtgtac
    32461 tcgtggcgat taatttcagt cctaaggttg gtgaaatact ggattgcaac tttggtaact
    32521 atcctgttag ccagaatgga cagttcagca ccacgtatta cgatggccgc attcctcctg
    32581 aaatgataaa aaacaggtta gt
//
LOCUS       DAZPJR010000029        32599 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPJR010000029 DAZPJR010000000
VERSION     DAZPJR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32599
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.030"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            358..921
                     /locus_tag="ACHVB7_001280"
     CDS             358..921
                     /locus_tag="ACHVB7_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1913312.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            1003..3645
                     /gene="yfcU"
                     /locus_tag="ACHVB7_001281"
     CDS             1003..3645
                     /gene="yfcU"
                     /locus_tag="ACHVB7_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFR1913313.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3665..4416
                     /locus_tag="ACHVB7_001282"
                     /pseudo
     CDS             3665..4416
                     /locus_tag="ACHVB7_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            4391..4939
                     /locus_tag="ACHVB7_001283"
     CDS             4391..4939
                     /locus_tag="ACHVB7_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1913314.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4936..5406
                     /locus_tag="ACHVB7_001284"
     CDS             4936..5406
                     /locus_tag="ACHVB7_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1913315.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5403..5927
                     /locus_tag="ACHVB7_001285"
     CDS             5403..5927
                     /locus_tag="ACHVB7_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1913316.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5914..6786
                     /locus_tag="ACHVB7_001286"
     CDS             5914..6786
                     /locus_tag="ACHVB7_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR1913317.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6908..7459)
                     /gene="smrB"
                     /locus_tag="ACHVB7_001287"
     CDS             complement(6908..7459)
                     /gene="smrB"
                     /locus_tag="ACHVB7_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR1913318.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7625..8557
                     /gene="prmB"
                     /locus_tag="ACHVB7_001288"
     CDS             7625..8557
                     /gene="prmB"
                     /locus_tag="ACHVB7_001288"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1913319.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            8592..9677
                     /gene="aroC"
                     /locus_tag="ACHVB7_001289"
     CDS             8592..9677
                     /gene="aroC"
                     /locus_tag="ACHVB7_001289"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR1913320.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9681..10505
                     /gene="mepA"
                     /locus_tag="ACHVB7_001290"
     CDS             9681..10505
                     /gene="mepA"
                     /locus_tag="ACHVB7_001290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR1913321.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10505..11314
                     /locus_tag="ACHVB7_001291"
     CDS             10505..11314
                     /locus_tag="ACHVB7_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR1913322.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11314..11862
                     /gene="epmC"
                     /locus_tag="ACHVB7_001292"
     CDS             11314..11862
                     /gene="epmC"
                     /locus_tag="ACHVB7_001292"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR1913323.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11896..12174
                     /locus_tag="ACHVB7_001293"
     CDS             11896..12174
                     /locus_tag="ACHVB7_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR1913324.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(12295..14301)
                     /gene="mnmC"
                     /locus_tag="ACHVB7_001294"
     CDS             complement(12295..14301)
                     /gene="mnmC"
                     /locus_tag="ACHVB7_001294"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR1913325.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            14460..15680
                     /gene="fabB"
                     /locus_tag="ACHVB7_001295"
     CDS             14460..15680
                     /gene="fabB"
                     /locus_tag="ACHVB7_001295"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR1913326.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15955..17133
                     /locus_tag="ACHVB7_001296"
     CDS             15955..17133
                     /locus_tag="ACHVB7_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1913327.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17130..18125)
                     /gene="flk"
                     /locus_tag="ACHVB7_001297"
     CDS             complement(17130..18125)
                     /gene="flk"
                     /locus_tag="ACHVB7_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR1913328.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            18224..19360
                     /gene="pdxB"
                     /locus_tag="ACHVB7_001298"
     CDS             18224..19360
                     /gene="pdxB"
                     /locus_tag="ACHVB7_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR1913329.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            19426..20439
                     /locus_tag="ACHVB7_001299"
     CDS             19426..20439
                     /locus_tag="ACHVB7_001299"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1913330.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            20439..21251
                     /gene="truA"
                     /locus_tag="ACHVB7_001300"
     CDS             20439..21251
                     /gene="truA"
                     /locus_tag="ACHVB7_001300"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR1913331.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            21334..21993
                     /locus_tag="ACHVB7_001301"
     CDS             21334..21993
                     /locus_tag="ACHVB7_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1913332.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            22149..23063
                     /gene="accD"
                     /locus_tag="ACHVB7_001302"
     CDS             22149..23063
                     /gene="accD"
                     /locus_tag="ACHVB7_001302"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR1913333.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            23133..24401
                     /gene="folC"
                     /locus_tag="ACHVB7_001303"
     CDS             23133..24401
                     /gene="folC"
                     /locus_tag="ACHVB7_001303"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR1913334.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            24391..25053
                     /gene="dedD"
                     /locus_tag="ACHVB7_001304"
     CDS             24391..25053
                     /gene="dedD"
                     /locus_tag="ACHVB7_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR1913335.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            25312..25800
                     /gene="cvpA"
                     /locus_tag="ACHVB7_001305"
     CDS             25312..25800
                     /gene="cvpA"
                     /locus_tag="ACHVB7_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR1913336.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            25837..27354
                     /gene="purF"
                     /locus_tag="ACHVB7_001306"
     CDS             25837..27354
                     /gene="purF"
                     /locus_tag="ACHVB7_001306"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR1913337.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            27449..28018
                     /gene="ubiX"
                     /locus_tag="ACHVB7_001307"
     CDS             27449..28018
                     /gene="ubiX"
                     /locus_tag="ACHVB7_001307"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR1913338.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            28284..29066
                     /gene="argT"
                     /locus_tag="ACHVB7_001308"
     CDS             28284..29066
                     /gene="argT"
                     /locus_tag="ACHVB7_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR1913339.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            29287..30069
                     /gene="hisJ"
                     /locus_tag="ACHVB7_001309"
     CDS             29287..30069
                     /gene="hisJ"
                     /locus_tag="ACHVB7_001309"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR1913340.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            30159..30845
                     /gene="hisQ"
                     /locus_tag="ACHVB7_001310"
     CDS             30159..30845
                     /gene="hisQ"
                     /locus_tag="ACHVB7_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR1913341.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            30842..31558
                     /locus_tag="ACHVB7_001311"
     CDS             30842..31558
                     /locus_tag="ACHVB7_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1913342.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            31566..32339
                     /gene="hisP"
                     /locus_tag="ACHVB7_001312"
     CDS             31566..32339
                     /gene="hisP"
                     /locus_tag="ACHVB7_001312"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR1913343.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            32536..>32599
                     /locus_tag="ACHVB7_001313"
     CDS             32536..>32599
                     /locus_tag="ACHVB7_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913344.1"
                     /translation="MTISTTSTPHDAVFKSFLRHP"
BASE COUNT         7656 a         8045 c         8776 g         8122 t
ORIGIN      
        1 aaatgccaat cgcattatat gttcattgtt tgttttttaa gctgttgata ttcattattg
       61 atatactttc tattttttct aaatacttat tccgtatttt ttaaggatgt ccttaagata
      121 acctttcttc tgtcaatata ttaattaaat ctacatttac tctttagtaa aattaaattg
      181 catcaggggt gtggaaacat cactatgcgg ccccacacca acgaatacaa ttttctacga
      241 cctgttcttt ttggatcgca tgccgtattc aatggctggg tataaacatt ggttattgac
      301 tgaatcaatg tattgcaatg tttatatttt taattattta tagtttaagg acaaataatg
      361 agtaagtttg ttaaaacagc tattgctgcg gcaatggtga tgggcgtgtt cacctctacg
      421 gcaacaatcg ctgcgggtaa caacggtact gcacgttttt acggcaccat tgaagactca
      481 gtgtgttcta tcgttccgga cgatcacaaa ctggaagttg atatgggtga tatcggtgcc
      541 gaaaaactga aaaataacgg caccaccacg ccgaaaagtt tccagattcg tctgcaagat
      601 tgcgtatttg atactcagga aacaatgacc actaccttta ctggtaccgt ttcttctgca
      661 aatagcggca attattacac cattttcaat accgatacct gtgcggcatt taacaatgtt
      721 agcctggcga tcggtgactc tctgggtacc tcttacaaaa gcggcatggg tattgaccag
      781 aaaatagtga aagacacttc taccaataaa ggcaaagcga agcagacact gaactttaaa
      841 gcctggctgg tcggcgcagc tgatgcgcca gatctgggta atttcgaagc aaataccacc
      901 ttccagatta cttacctgta aggcataccc ccatagcgac actgctatgg gggatttaaa
      961 tggattttga ccgaacaaga ataaaaacgg atagaaacgt gtatgcctga ccattctctt
     1021 tttcgattac ggattcttcc gtggtgcatt gcgctggcaa tgtcagggag ttatagcagt
     1081 gtctgggctg aagacgacat tcagtttgat tcccgttttc tggaattaaa aggcgacaca
     1141 aaaattgatc tgaagcgttt ttccagccag ggatatgttg agcccggaaa atacaattta
     1201 caggttcaac taaataaaca gccattggcg gaagagtacg atatttactg gtatgctggt
     1261 gaagatgacg cgagcaaaag ctatgcttgt ctgacaccgg aactggtagc gcagtttggt
     1321 ttaaaagaag acgtggcgaa aaatctgcaa tggagccacg atgctaaatg cctgaaatcc
     1381 ggtcaactgg aaggcatgga aattaaggct gatttaagcc agtccgcatt agtcatttca
     1441 ctgccacagg cttacctcga atatacttat cccgactggg atccgccttc acgttgggat
     1501 gacggcatct ccgggatcgt cgcggactac agcatcaacg cacaaacccg gcacgaagaa
     1561 aatggcggtg atgatagtaa cgagatcagc ggcaacggga cggtcggggt taacctgggg
     1621 ccgtggcgta tgcgtgctga ctggcagact aactatcaac atactcgcag taatgatgac
     1681 gatgaattca gcggcgatga aactcaaaaa aaatgggagt ggagtcgcta ctatgcctgg
     1741 cgggcgttac catcattaaa agccaaactg gcgctgggcg aggattacct cagatccgat
     1801 atttttgatg gttttaacta tgttggtggc agtgtcagta ctgacgatca aatgttgcct
     1861 cccaatctgc gcggctacgc gccagacatt tccggcgtgg cacacaccac agcaaaagtg
     1921 accgtcagcc agatggggcg tgtgatttac gaaacgcagg ttccggctgg accgtttcgt
     1981 attcaggatc ttggtgattc tgtctccggt acgttgcata ttcgcattga agaacagaac
     2041 ggccaggtgc aggaatatga catcagcacc gcctcgatgc catacctcac tcgtccaggt
     2101 caggttcgct ataagatcat gatgggccgt ccgcaagagt ggggacacca tgtcgagggt
     2161 gaattttttt ctggtgctga agcttcctgg gggatcgcta acggctggtc gttatatggc
     2221 ggcgcactgg gagatgaaaa ctatcagtct gcggcgcttg gcgtcggtcg cgatttgtct
     2281 acattcggcg cggtcgcgtt tgatgttact cactcgcaca ccaaactgga taaagacacc
     2341 gcttatggca aaggttcgct ggacggtaac tccttccgtg tgagttattc caaagacttt
     2401 gaccagctca acagccgcgt tacttttgct ggatatcgct tctcggaaga gaactttatg
     2461 accatgagcg agtatctgga tgccagtgac agcggaatgg tacgcacggg caacgacaaa
     2521 gagatgtaca ctgccactta taaccagaac ttccgcgatg cgggtgtttc ggtttatctc
     2581 aactataccc gccataccta ctgggatcgc gaggagcaga caaactacaa catcatgctc
     2641 tcgcactatt tcaatatagg cagtattcgt aatgtcagca tctcgatgac tggctaccgt
     2701 tacgagtatg acaaccaggc cgacaaaggc atgtacattt cgctcagtat gccgtggggt
     2761 gacaacagta ccgttagcta taacggtaac tatggcagtg ggacggacag cagtcaggtc
     2821 ggttatttca gccgtgtcga tgacgcgact cactatcagt tgaacgtcgg caccagtgac
     2881 aaacacacca gcgttgacgg ctattacagc catgatggtt cgctggcgca ggttgacctc
     2941 agtgcgaact accatgaagg gcaatacacc tctgcgggct tgtcgttaca gggcggcgca
     3001 acgcttactg cccacggtgg cgcacttcac cgtacccaga atatgggcgg gacacgcttg
     3061 ttgattgatg ccgatggcgt tgccgatgtt ccggtagaag gtaacggtgc tgctgtttat
     3121 accaatatgt ttggtaaagc cgtcgtttct gacgtcaata actattaccg caatcaggcg
     3181 tatatcgacc tcaacaaact gccggaaaac gccgaagcaa cccagtcggt ggtacaagcc
     3241 acgctaactg aaggggcgat tggctaccgc aaatttgccg tcatcagtgg tcaaaaagcg
     3301 atggcggtgc tgcgtttaca agacggcagc catcctccgt ttggcgcaga agtaaaaaat
     3361 gataacgagc agacagtggg ccttgtcgat gatgacggca atgtttatct ggctggggtg
     3421 aaacctggcg aacacatgag tgtgttctgg agtggtgttg cgcattgcga tatcaacctg
     3481 ccggacccgc tgcctgccga tctgtttaac ggcttgttac tgccatgcca gcataaaggc
     3541 aacgtcgcgc cggttgttcc tgatgacata aagcctgtca ttcaggagca gacgcaacag
     3601 gtgacaccca ccaatccccc tgtttctgtt tcagctaacc aataacgtga ttaaggaatg
     3661 atccatgtct gatttacttt gctctgccaa attgggtgct acgacgctag cgttactgct
     3721 tagcgctgcc agtttgtcgc ccaggcttct gtaacgccag atcgtacccg tttgatattt
     3781 aatgaaagcg acaaatcaat cagcgtgacg ttgcgcaata acgatccgaa actgccttat
     3841 ctggcccaaa gctggatcga agatgaaaag ggtaacaaga tcagttcacc gttaactgtt
     3901 ctgccaccgg tgcaacgcat cgattcaatg atgaacgggc aggtaaaagt gcagggaatg
     3961 ccggatatca acaaattgcc tgccgatcgt gaaagcctgt tctacttcaa cgtgcgtgag
     4021 atcccgccaa aatcaaacaa agccaacacg ctgcaaatag cactacaaac gcgtattaag
     4081 ttgttctggc gaccgaaggc gctggagaac gtcagcatga aaaacccctg gcaatataag
     4141 gttactttaa cccgcaacgg tcaggaattt accgtcaata acccgacgcc gtattacgtc
     4201 attttcagta atgccagcac acagaaaaat ggcaatcctg cagccggttt ctcgccaata
     4261 gtgatgtcgc cgaaaacctc tgcaccgctg aatgtgaaga tgggtagcgt gccggtactg
     4321 acctatgtca acgactatgg cgctcggatg ccgctctttt tcacttgcaa cggtaacagt
     4381 tgccaggtcg atgaagagca gagccgcaaa ggctgaggcc gggagagcca gtatgaaagg
     4441 atcttgttct gtctgggcgg taactgggtt actgatatgt tgtgttggta gcggctttgc
     4501 agttgcagcg agtaagtcag taagtatgaa tttacgggtg cttgtcgatg cccctcctcc
     4561 ttgtacggta aatggtgcgg cagttgagtt tggtaatgta ttcattaata agatcaatgg
     4621 ggttgattat aaacgtccca ttgactactc gcttgtttgt aacaatctgg caatggatga
     4681 cctgcgtcta cagatgcagg cgacaacggt cgtgattaac ggtgaaacag ttatcagcac
     4741 gggtattcct gggtttggta ttcgggttca gaaaagtagt gatcacacaa tactcgactt
     4801 aacctctggc tcatggttgc catttaattt ttcctcggga gttcccgttc tggaagctgt
     4861 gccggtgaaa cagagtggta caacgttagc ggcagctgaa tttaacgcca gtgccacaat
     4921 tgtggtggat taccaatgaa acggatctcg cttattttgc tgtggggctt ttgctcgatg
     4981 gcactctcaa acgtctcttt tcacggttat ttagtgcagc cgcctaactg cacgattagc
     5041 aacgctcaaa ccattgaaat aacttttcag gatgtgctta ttgatgacat taatggcagc
     5101 aattatgagc aaaccgtgcc gtattccatt acctgcgata cggcggttcg cgacccatta
     5161 atggaaatga ccttgagctg gagtggcaca ctaagcgatt ttgataatgc tgcagtaagc
     5221 agcaatatca ctggtttagg cattcagctc aaacaggcag ggcagagttt tacgatcaat
     5281 acgccactta tggtgaatga aaccgattta ccggtgttaa cggctgtgcc agtaaaaaag
     5341 agtggtgtcg ttctgccaga ggcggacttt gaggcctggg ccacgttaca ggtagattac
     5401 caatgaagaa aaagcgtacg ttgtttttta ttagtagcct gatgttgctg ggcagtggaa
     5461 ccacaatagc aggagataat ttgcacttta ccggcaattt aatcagtaaa tcgtgtacgc
     5521 cagtgattaa tggtagccag ttggcagaag tacattttcc tgccattgcc gccagcgatt
     5581 taatgaacct tgggcagtca gagcgagtgc cattggtgtt tcaattgaaa gattgtcata
     5641 gttctacatt gtttaatgtc aaagtaacgt tgacaggaac agaagatagt gctctacccg
     5701 gctttctggc ctttgactcc tcgtcatcag catccggcgc tgggattggt attgaaactg
     5761 cggcaggcac atcggtgcca atcaacaata caacaggcgt cacgctcccg ctgaatcagg
     5821 gaaataacag cctaaatttt aatacctggt tacaggcaaa gagcgggcga gatgtaacgt
     5881 ccggagactt tagtgccaca gtgacggcaa cctttgagta tttctgaaat gaaaaaatgg
     5941 accataattt tcgcgagcct aatgctgctc gttctgagtg ttgtcggcgc atctaagagt
     6001 tatgctggag ataaacttat gagtgcgagt tttgacagtg tgagagttta ttatgccatg
     6061 gacaaggtaa ccggtgctgt agcgagcagt gttttcaatg ttacggtgat aacgccaaag
     6121 gaggttacat atgggaaata tgattctttt gcgtataaag gcgatacgtt gcgggttatt
     6181 tcctggtctg ggtcgggttc agctccaact ttggtactga ctgactttga tacaattaat
     6241 aactcaaatt gccctggcat agatacgaaa atttttcgtt gtgcatatat gacgtttaaa
     6301 attaccgttg catctgatga ttatggttgc ccttggatcg catcctttta ttcctataca
     6361 gatttacccg gatttggaag ctatacggcc cctacagtgc ataacacaat atgcccaacg
     6421 ataccggtca cttcatacga tatctcctgg agtgaaaatt atgtcagcca taataaggcg
     6481 ttacgaattc agtccacggg gtcaacagtg accaccacgc tttcgaccta cctgatggaa
     6541 ggcggcagac tctgtgatgg cagtaatttt tctgataatg atggcagagg agcatactgc
     6601 cgcgcggtat cagaattgtt aacctttacc tcttacggtt gtgataaatc aacggtaacg
     6661 gttacgccaa ctcgtcatcc ggtaacggat aaagtactac acgatattgt ggtgaatgtg
     6721 aataccagta gtgggcaacc gattgattcg acgtgtcgtt ttcagtatgt attgaatgaa
     6781 ctgtagttcg tagtattgct ctgatgcgtc ttatcaggcc tacgaagtga attgcaattt
     6841 attgaattat cacatatttg taggccggat aaggcgttta cgctgctccg gcatttgctg
     6901 ccagaattca gggcaactcc ggcggcaacc actcttccac ttcaatcaac accaacaaag
     6961 ccgcatcacc gccgtactct ttcggtgcct ggtgaaatgc catcacatgc ggatgttgtg
     7021 ccagccacag cggtgtttgt tgcttcaaaa tatgcttccc gtgcccatgc atcacgcagg
     7081 cacaaaacac atgttcgcgg cgacaggcgg caatcaacgc gcccagttcc tgcttggcct
     7141 gcagctgcgt cagaccgtgt aaatccaaaa acaattccgg tgaataatcg ccgcggcgca
     7201 gtttcttcgc ctcaaaatgg ctgacatccg ggcgaacata tttcaccgga ccttcggtat
     7261 ttaataacgg ctgaaactca tcggagaaat aatggctggc atcagcctgc tccgggatca
     7321 agcgtttcac cggcacttcg ctgatttttt tacgctgcgg tcggtggaca atcgtgtcct
     7381 gcttaatctt gcgagtcccc gccatcaact ggcgaaacag agcctggtcc tcctcgctaa
     7441 gtgttgtttt ctttttcatt tatctgtctc tatttctaat tttcctacag tttacccgac
     7501 tcgtagcaca atcgatcgtt ttaaacgtat ttttcctcac tcaccgcgcg tgaatgctga
     7561 tttatcgtca tcttcatggc aaactagccg ccgaaattat tgcgatcatg ccctggagga
     7621 atacgtggat aaaattttcg ttgatgaagc agtaaatgag ctgcaaacca ttcaggacat
     7681 gttgcgctgg tcggtaagcc gcttcagcgc ggcaaatatc tggtacggtc acggcaccga
     7741 taacccgtgg gatgaagccg tacagctggt gttaccatcg ctctacctgc cgctggatat
     7801 cccggaagat atgcgcaccg cgcgtctgac ctccagcgaa aaacaccgta ttgttgaacg
     7861 cgtgatccgc cgcgtcaacg agcgcattcc ggtggcttac ctgaccaaca aagcctggtt
     7921 ctgcggtcat gaattttacg tcgatgaacg cgtgctggtg ccgcgctcgc cgattggtga
     7981 actgatcaac aataaatttg ccggactcat tagcaagcaa ccgcagcata ttttagatat
     8041 gtgtaccggt agcggctgca tcgccattgc ctgtgcttat gccttcccgg aagcggaagt
     8101 cgacgcggta gatatctctc ccgacgcgct ggcggttgcc gaacagaaca tcgaagaaca
     8161 cggtctgatc cacaacgtca ttccgattcg ttccgatctg ttccgtgatt tgccgaaagt
     8221 gcagtacgat ctgattgtca ctaacccgcc gtatgtcgac gcggaagata tgtccgacct
     8281 gccaaacgaa taccgtcacg agccggaact gggcctggca tctggcaccg acggccttaa
     8341 actgacgcgt cgcattcttg gaaacgcggc agattacctt gctgatgatg gcgtgttgat
     8401 ttgtgaagtc ggcaacagca tggtacatct tatggaacaa tatccggatg ttccgttcac
     8461 ctggctggag tttgataacg gcggcgatgg tgtgtttatg ctcaccaaag agcagctgct
     8521 ggcggcccgt gaatatttcg cgatttataa agattaagta aacacgcaaa cacaacaata
     8581 acggagccgt gatggctgga aacacaattg gacaactctt tcgcgtaacc accttcggcg
     8641 aatcgcatgg gctggcgctc ggctgcatcg tcgatggtgt tccgccaggc attccgctga
     8701 cggaagcgga cctgcaacat gacctcgacc gtcgtcgccc tgggacatcg cgctatacca
     8761 cccagcgccg cgagccggat caggtcaaaa ttctctccgg tgtttttgaa ggcgttacta
     8821 ctggcaccag cattggcttg ttgatcgaaa acactgacca gcgctctcag gattacagcg
     8881 caattaaaga cgttttccgc ccaggccatg ctgattacac ctacgaacaa aaatacggtc
     8941 tgcgcgatta tcgcggcggt ggacgttctt ccgcccgcga aaccgccatg cgcgtggcgg
     9001 caggggcgat tgccaaaaaa tatctcgccg agaaatttgg tattgaaatc cgcggctgct
     9061 tgacccagat gggcgacatt ccgctggaaa tcaaagactg gtcgctggtt gaacaaaatc
     9121 cgttcttctg cccggacccg gacaaaatcg acgcgttaga tgaactgatg cgcgcgctga
     9181 aaaaagaggg cgactccatc ggcgcgaaag tcaccgttgt tgccagtggt gtccccgccg
     9241 gacttggcga gccggtcttt gaccgcctgg atgccgacat cgcccatgcg ctgatgagca
     9301 tcaacgcggt gaaaggcgtg gaaattggcg acggttttga cgtagtggcg ctgcgcggca
     9361 gccagaaccg cgacgaaatc accaaagacg gtttccagag caaccatgcg ggcggcattc
     9421 tcggcggtat cagcagcggg cagcaaatca ttgctcatat ggcgctgaaa ccgacctcca
     9481 gcattaccgt gccggggcgc actattaacc gctttggcga agaagttgag atgatcacca
     9541 aaggccgtca cgatccctgt gtcgggatcc gtgcagtgcc gatcgctgaa gcgatgctgg
     9601 cgatcgtttt aatggatcac ctgttacggc agcgggcgca aaatgccgat gtgaagactg
     9661 atattccacg ctggtaaaaa atgaacaaaa ccgcgattgc gctgctggct ctgcttgcca
     9721 gtagcgctag cctggcagcg acgccgtggc aaaaaataac ccaacctgtg ccgggtagcg
     9781 cacaatcgat aggcagtttt tctaatggct gtattgtcgg cgctgacacg ttgccgatac
     9841 agtccgaaca ttatcaggtc atgcgtaccg atcagcgtcg ctatttcggt cacccggatc
     9901 tggtgatgtt tatccagcgt ctgagtagcc aggtgagcaa tctgggcatg ggtacggtgc
     9961 tgattggcga tatggggatg cccgctggtg ggcgtttcaa cggcggtcat gccagccacc
    10021 agaccggact ggatgtcgat atctttctgc aactgccgaa aactcgctgg acctccgcgc
    10081 agctcttgcg cccgcaagca ctggacttag tttcccgcga cggtaaacac gttgtctcca
    10141 cgctgtggaa gccagaaatt ttcagcttga tcaaactcgc tgcccaggac aaagacgtca
    10201 cgcgcatttt tgttaatccg gcgattaaac aacaactttg ccttgatgcg ggcaccgatc
    10261 gcgactggtt gcgcaaagtg cgaccctggt tccagcatcg cgcgcatatg catgtacgat
    10321 tacgttgccc tgccgatagt ctggagtgtg aagatcaacc tttaccgcca tcaggcgatg
    10381 gttgcggggc agaactgcaa agctggtttg cacctctaaa accgggaaca acaaagcctg
    10441 agaagaagac accgcctccg ttgccgcctt cctgccaggc gctactggat gagcacgtga
    10501 tctaatggaa acgtttaata gcctgtttat ggtttccccg ctgttgctgg gagttctctt
    10561 ttttgtcgcc atgctggcgg gatttatcga ctcgattgcc ggtggcggtg ggttactcac
    10621 cattccggca ttgatggctg cgggaatgtc tcccgctaac gcgctggcaa ccaataaact
    10681 gcaagcctgc ggcggctcta tttccgccac tatctacttt attcgccgca aagtggttag
    10741 tttaagcgat cagaaactca atattgccat gacctttgtc ggctcaatga gcggcgcact
    10801 gttggtacag tacgttcagg ctgatgtctt acggcagatt ttgcccattc tggtgatttg
    10861 tatcggcctc tatttcttgc tgatgccaaa gctgggtgaa gaagatcgcc agcgccggat
    10921 gtacggttta cccttcgcgt taattgccgg tggctgcgtc ggtttttatg acggattctt
    10981 tggcccggct gccggatcgt tttacgcgct ggccttcgtt acgctgtgcg gattcaacct
    11041 cgccaaagcc acggctcacg ccaaattact caacgcaacg tcaaacattg gcggtctgct
    11101 gctgtttatt ctcggcggca aagtgatttg ggcgacgggc tttgtcatgc tggtggggca
    11161 gttcctcggc gcgcgcatgg gttcacggct ggtgttgagc aaaggacaaa agctgatccg
    11221 cccgatgatt gttatcgtct cggcggtgat gagtgccaaa ttactttacg atagccacgg
    11281 acaggagatc ctccactggt tggggatgaa ctaatgaaca gtacacacca ctatgagcag
    11341 ttgattgaaa tttttaatag ctgctttgcc gatgaattta atacccgtct gattaaaggc
    11401 gacgacgaac cgatctatct tcctgctgat gcggaagtac cgtataaccg aattgtcttt
    11461 gcccacggct tttatgccag cgcaattcat gagatttcgc actggtgtat tgccggaaaa
    11521 gcgcgccgtg agctggttga tttcggctac tggtattgcc cggatgggcg tgatgcccaa
    11581 acgcaaagcc agtttgaaga tgttgaagtg aagccgcagg cactcgactg gttgttctgt
    11641 gtggcggcgg gatatccgtt taacgtcagt tgcgacaatc tggaaggcga cttcgaaccg
    11701 gatcgtgttg tgtttcaacg gcgggtacat gcgcaggtga tggattatct gacaaatggc
    11761 attcccgagc gtccggcacg cttcattaaa gcactacaaa attattatca cacgccggaa
    11821 cttacggcgg aacagttccc gtggccggaa gcgctcaact gaagccggtg ccacgtttta
    11881 caatgaggaa agaagatgat cgcggagttt gaatcacgca ttctggcatt aatcgacggc
    11941 atggttgacc atgccagtga tgatgagttg tttgccagtg ggtatttgcg tggccacctg
    12001 acattagcca tcgcagaact ggaaagtggt gatgaccact ccgctcaggc ggtgcatacg
    12061 accgttagcc agagtctgga aaaagccatt ggtgccggtg aattgtcgcc gcgtgaccag
    12121 gcgctggtga ccgatatgtg ggaaaacctg tttcagcagg cgtcacagca gtaagttgcg
    12181 gcgtctggag caccatgcct gatgcgacgc ttatcgcgtc ttatcaggcc tacacgtcac
    12241 acgtaggtcg gataaggcgt tcacgccgca tccggcaaac actgcgagag cagattaccc
    12301 cgccttaacc gctttaccct tcaacaattt ccgcacccat aaccgattcg ggtttaacgc
    12361 cgtcagcgta ctggcatcca tcggaatcgg ttcgtcgctc atctgcgccg ccagaatctc
    12421 ggcacacagc ggggcagaac acaaaccgcg agaacctaaa gccgcaaaca taaagagatc
    12481 gtcaaaaacc ggcgcgctta ccgcctcatc tttctgctcc gccaacgacg catattccac
    12541 gagtgttgcc tcataatcgg gaacattgcc taccattggc agatgatcgc gggtggcaca
    12601 acgcacaccg cagcgcgcct ctttatcact gacatcaacc tcttttgccc actgtgcctg
    12661 cgggaaacaa tcaatcaacc gctggcgatt ctgctgctga tcgtcctcac tgtacgccgt
    12721 atcttcgctg ccgcgatgat aactggcacc aatacaatga tgttgattcg ccggattttg
    12781 tggcgtgaga taaccgtcat agcacagcac ctgcttcagc tctgccagtt ccggcgttgt
    12841 cggaatatgg ctgacctgcc cggcaaccga atacaccggg agagtcgacg tttggctgaa
    12901 acggctgatt tgatgcccgt tcgccagtac cactacgctg tgtgttgctt gctgatctcc
    12961 tgcaaaattc aacaaccaac agtcatcctt acgggataaa ttctgtaact gatattgata
    13021 ataaatctgc aaaccctgct gttgcgccag ttccagcaca ttacgggtca gttctgctgg
    13081 gcacagccaa ccaccttgcg gataagtaat gccgctgcaa tttgtcgcaa cgcccgtaat
    13141 ttgttcaacc gcatttgcct caacggctac agccagttct gcgggtaaat ccattgacaa
    13201 catctgtgcg attttatgct ggcttttctc atcccagcct aactgcgtga cgccgcacca
    13261 gtcatgatca aatttaacgg gtaattggtc gtaaaaccga cgagcaaaag taaacgcatt
    13321 agagaaaaag cggtttagcg cctcatcgtg tttgcttaat aacggataca gcgccccctg
    13381 gcgattgccg gaagcaccca gcgcgggggc ttcatccgcg caataaagcg ctacctgcca
    13441 gccgcgccgt aataacgcca gcgacaacaa cgcgctggca ataccaccgc cgataatcgc
    13501 tacttcccgt ttgctgctgc ccgtacgatt aaaccacggc gtggagcagg ggagtggtaa
    13561 tgtctgttcc atcaccccac aaagcatttc ccgtttgcgc ccaaagccct tacgtttttg
    13621 catcgtgaat ccggcttcct gcaaaccgcg gcggacaaaa ccggcagacg taaatgtcgc
    13681 cagcgtgccg cctggacgcg ccaaccttgc catggcgtta aacagatttt gcgtccacat
    13741 atccgggttt ttagctggcg caaagccgtc cagaaaccag gcatctactt tttgatttag
    13801 cgaatcgtcc agttggctgg tcagttcgtt aatatcgcca aaccataaat ccagcgtcac
    13861 gcggccttca ttgagcaata aacgatggca accgggcaag ggcattggcc actgcgcctg
    13921 aagttgttct gcccacggag ccagttccgg ccagtgttga tgcgctaagg ctaaatccgc
    13981 acgggtgagg ggaaatttct caaaactaat gaaatgtaag cgttgtaatt gcgcttgcgg
    14041 atgcgcttcg cgaaactgat caaatgcctg ccatagcgtc aggaagttta atccggtgcc
    14101 gaagccgctc tctgctacca caaacagagg atgtggatgc tcaggaaagc gtacctctaa
    14161 ttggttgcct cccagaaaaa cataacgcgt ctcttccagc ccgttatcgt tggaaaaata
    14221 gacatcgtca aaatctcggg aaacaggtgt accctcagca ttaaattcga ggttggcagg
    14281 ttgtatggag tagtgtttca cgtaagttac tcgtcttaca ggcggtggct cgatcttagc
    14341 gatgtgtgta aggctgcgca aatttctcta ttaaatggct gatcggactt gttcggcgta
    14401 caagtgtacg ctattgtgca ttcgaaactt actctatgtg cgacttacag aggtattgaa
    14461 tgaaacgtgc agtgattact ggcctgggca ttgtttccag catcggtaat aaccagcagg
    14521 aagtcctggc atctctgcgt gaaggacgtt cagggatcac tttctctcag gagctgaagg
    14581 attccggcat gcgtagccac gtctggggca acgtaaaact ggataccact ggcctcattg
    14641 accgcaaagt tgtgcgcttt atgagcgacg catccattta tgcattcctt tctatggagc
    14701 aggcaatcgc agatgcgggc ctttctccgg aagcttacca gaataacccg cgcgttggcc
    14761 tgattgcagg ttctggcggc ggttccccgc gtttccaggt attcggcgct gacgcaatgc
    14821 gcggcccgcg cggcctgaaa gcggttggcc cgtatgtggt caccaaagcg atggcatccg
    14881 gcgtttctgc ctgcctcgcc acgccgttta aaattcatgg cgttaactac tccatcagct
    14941 ccgcgtgtgc gacttccgca cactgtatcg gtaacgcagt agagcagatc caactgggca
    15001 aacaggacat cgtgtttgct ggcggcggcg aagagctgtg ctgggaaatg gcctgcgaat
    15061 tcgacgcgat gggtgcgctg tctactaaat acaacgacac cccggaaaaa gcctcccgta
    15121 cttacgacgc tcaccgtgac ggtttcgtta tcgctggcgg cggcggtatg gtagtggttg
    15181 aagagctgga acacgcgctg gcgcgtggtg ctcacatcta tgctgaaatc gttggctacg
    15241 gcgcaacctc tgatggtgca gacatggttg ctccgtctgg cgaaggcgca gtacgttgta
    15301 tgcagatggc gatgcatggc gttgataccc caatcgatta cctgaactct cacggtactt
    15361 cgactccggt tggcgacgtg aaagagctgg cagctatccg tgaagtgttc ggcgataaga
    15421 gcccggcgat ttctgcaacc aaagccatga ccggtcactc actgggcgct gcgggtgtgc
    15481 aggaagctat ctactctctg ctgatgctgg aacacggctt tatcgccccg agcatcaaca
    15541 ttgaagagct ggacgagcag gctgcgggtc tgaacatcgt gaccgaaacg accgatcgcg
    15601 aactgaccac tgttatgtct aacagcttcg gcttcggcgg caccaacgcc acgctggtaa
    15661 tgcgcaagct gaaagattaa ttcgcagtag gccggagtag gtcggataag acgcgccagc
    15721 gtcgcatccg acgttacgcg ccaatgcggc ctccggcact aacgcaaaag ggaacctgat
    15781 ggttcccttt ttcacatcat tgacaatcgc caccagttcc aggcaaactt cccgctttgt
    15841 cgatttcctt ctgaaaagac gtacgcgtta aatcctgcca acgcaccgta accctgaaac
    15901 cagagagatg agacggggat actcctcgcc ttgcgctgca ttctggagta atgcatgact
    15961 gctgtaagcc aaaccgaaac acgatcttct gccaattttt cgctcttccg catcgctttt
    16021 gcggtttttc tcacctacat gaccgtaggg ctgccgttgc cggttatccc gctgtttgtt
    16081 catcatgaac tgggctatgg caataccatg gtcggcattg ccgtcgggat tcagtttctg
    16141 gctacggtgc tgacgcgtgg ctatgccggg cgactggccg atcaatatgg cgcaaaacgt
    16201 tcggcgcttc aggggatgtt agcttgtggt ctggctggcg gcgcgttgct gctggcggcg
    16261 attttgcctg tctccgcacc gttcaaattt gccctgttgg tcatcgggcg tttgattctt
    16321 gggtttggtg aaagccagtt actgacaggc gctctgacct gggggttagg catcgtaggg
    16381 ccaaaacact ctggcaaagt gatgtcatgg aatggaatgg cgatttacgg tgccctcgct
    16441 gttggtgctc cgcttggcct gttgattcat agccattacg gttttgccgc actggcgatc
    16501 accacaatgg tattaccctt actggcgtgg gcctgtaacg gcacagtgcg caaagtaccg
    16561 gccctggcgg gagaacgtcc atcgctgtgg agcgttgtcg ggtttatctg gaaaccaggg
    16621 ttagggctgg cactacaagg cgttggtttt gcggttatcg ggactttcgt ttcgctctac
    16681 tttgccagca aaggatgggc gatggcgggc tttactctta ccgagtttgg cggcgcattt
    16741 gtcgtgatgc gcgtcatgtt tggctggatg ccggaccgtt ttggcggcgt gaaagtggcg
    16801 attgtctctc tgcttgtaga aacggtgggc ttgttgctgc tctggcaagc cccaggtgcg
    16861 tgggtcgcat tagcgggcgc ggcgttaacc ggagccggat gttcgcttat ctttcctgcg
    16921 ctgggcgtgg aggtggttaa acgcgtcccc tcacaagttc gcggcaccgc actgggcggt
    16981 tacgccgcgt ttcaggatat cgccctcggc gtctccgggc cgctggcggg aatgctggcg
    17041 accacgtttg gttactcttc ggtatttctt gccggggcga tctctgcggt gctgggaatt
    17101 attgtcacga tactgtcatt tcgtcggggc taacgaacca gccagaccag catcaggata
    17161 atcgcgacta acagtatcca cagcgcggga cgcgttgcca tattttgcaa cgtgtccatc
    17221 atcggtggaa aaggattaag cagcggttgc atatcacgcg gatcaatccc tttaacccgc
    17281 cgttgataaa gctgattaag aatatcctcg gcctgggcag aagaaagcga tgattgcgca
    17341 gaaagaccat atttttgctg gatatacgcc gataacgctg ccagttcact ggcatctaaa
    17401 ggttgtttta gcgccatctg tagtgattcc agcgtcggcg tattttgctg gcttaacgtc
    17461 tggcgcgcct gcaaccaggt caccagatgg ttaaacagtt tcgctggaat taactcgcca
    17521 tcgttcaccc cggaaagttc cagcatcgat tgccagatct gtttgctggg ttcccccgtt
    17581 gccgccgcaa gtttggtcac cagctgtttt agcgcattgt gctccgccgg taataaagga
    17641 cggtcggtcg cctcgcgctg ctgtggttgc ggaataacca tcttcccttc ctgcaacagg
    17701 gtgagaatgg tttttaattg ctccggtgag agctgattca gcggcgtctg accaaagtta
    17761 tgacggatat aatccgtgac cgcctgacga ttattcccca gacgtaaata ctcccctaat
    17821 tgcgctaaaa gctggcgggc agaatggctt ttttgcgcgg ccagcagacg ttgcgccaga
    17881 ttatgctcag cggcagggaa gtgacgcgaa agcagcggtg aatctcccga cagaccaata
    17941 tcgtgcctga tacccgccca cagttctgct ctttgttgtt gcgtcagtga ggtcactttc
    18001 gtcattaagc tttccagcga agtacgttgc tgactggata aaggctgatt gcccgcgcca
    18061 gacggcaggt tatctccctg acctggtggt tgcccaggag gagggccgga aataggttgt
    18121 atcattacgt atccttatac ctgaaatctt cgcaagtatg cctggccgcg agattatggc
    18181 acacttgtcc ggttaactct cgtctcatac aggtaacaca aacgtgaaaa tccttgttga
    18241 tgaaaatatg ccttatgccc gcgacttatt tagccgtttg ggtgaggtga ccgcggttcc
    18301 cgggcgtcca atccccgtcg ctcaactggc agacgcggat gcgctgatgg tgcgttcggt
    18361 cacgaaagtg aatgaatctt tgctggcagg aaaacccatt aaatttgttg gcactgccac
    18421 tgcggggacc gaccatgtcg atgaagcatg gttgaagcag gcgggaattg gtttttccgc
    18481 tgcacctggc tgtaatgcga ttgcggtggt ggaatatgtt ttctcctcac tactgatgct
    18541 tgccgaacgc gatggatttt cactgcacga ccgtacggtg gggatcgtgg gcgttggtaa
    18601 cgttggacgt cgattgcagg cgcgactgga agcgttaggg attaaaacat tgctttgcga
    18661 tccgcctcgc gctgaccgtg gggatgaggg tgatttccgc tcgctggatg aattagttca
    18721 gcatgcggat attctgactt tccatacgcc actcttcaaa gatgggccgt acaaaacgct
    18781 gcatctggcg gatgaaaaac tgatccgtag cctgaagccg ggagcgattc tgattaacgc
    18841 ctgccgtggc gcagtcgtcg ataatactgc gttgctgacc tgcctgaatg aaggccagaa
    18901 gttaagcgta gtgctggatg tctgggaagg cgaaccggaa cttaacgtgg agttgctgac
    18961 aaaagtggat atcggcacgc cgcatatcgc aggttatacc ctggaaggta aagcacgcgg
    19021 tactacgcaa gtgtttgaag cctatagcaa gtttattggg catgaacagc acgttgcgct
    19081 ggatacatta ctgcccgcgc cagagtttgg tcgcattacg ctgcatggcc cgctcgatca
    19141 accgacgctg aaaaggctgg tgcatttggt gtatgatgtg cgccgcgatg acgcaccgct
    19201 gcgtaaagtc gccgggatac cgggtgagtt cgataaactg cgcaaaaact atcttgagcg
    19261 ccgtgaatgg tcatctctgt atgtaatttg tgatgacgcc agtgcggcat cattgctgtg
    19321 taaactgggt tttaacgccg ttcatcatcc ggcacgttaa tctcttcttc atgctctctg
    19381 ctgtaacatt ggcagggagc tttgctattt ctggagtaaa ccaccatgtc tgaaggctgg
    19441 aacattgccg tcctgggcgc aactggcgct gtgggcgaag ccctgcttga aacgctggct
    19501 gaacgtcagt tcccggttgg ggaaatttat gcactggcac gtaacgaaag cgcaggcgaa
    19561 caactgcgct ttggtggtaa aacaatcact gtgcaggatg ccgctgaatt cgactggacg
    19621 caggcgcagc tggcattttt tgtcgcaggc aaagaagcta ccgctgcctg ggttgaagaa
    19681 gcgaccaact caggttgcct ggtgatcgac agcagcggac tgtttgctct cgaacccgac
    19741 gtaccgctgg tggtgccgga agtaaacccg tttgtactga ccgattaccg caaccggaat
    19801 gtcatcgccg taccagacag tctgaccagc cagctgctgg cggcactgaa accgttaatc
    19861 gatcagggcg gtttgtcgcg tatcagtgtt accagcctga tttcagcctc cgcccagggc
    19921 aaaaaagcgg tcgatgcgtt agcggggcag agtgcgaaat tgctcaacgg cattccgatt
    19981 gacgaagaag atttcttcgg tcgtcaactg gcgttcaata tgctgccgtt gttgccggat
    20041 agcgaaggta gcgtgcgtga agaacgtcgt atcgttgacg aagtacgcaa aatcctgcag
    20101 gacgaagggc tgatgatttc ggcaagcgtc gtccaggcac cggtattcta cggtcatgcc
    20161 cagatggtca actttgaagc actgcgtccg ctggcggcag aagaagcgcg tgatgcgttt
    20221 gctcagggcg aagatattgt gctctctgaa gagaacgaat tcccgactca ggtaggggat
    20281 gcttcgggta cgccgcatct ttctgttggc tgcgtgcgta atgactacgg tatgccggag
    20341 caagtccagt tctggtcggt ggccgataac gttcgctttg gcggcgcgct gatggcagta
    20401 aaaatcgccg agaaactggt gcaggagtat ctgtactaat gtccgaccag caacaactgc
    20461 cagtttataa aattgcgctg ggcattgagt acgacggcag taagtattac ggctggcaac
    20521 ggcagaatga agtccgcagt gtgcaggaga agctggaaaa ggcgctctcc caggtggcga
    20581 acgaacccat caccgtcttc tgcgccgggc gtactgacgc aggggtacac ggtaccgggc
    20641 aggtggtgca tttcgaaaca accgcgcagc gcaaagacgc ggcgtggacc ttgggcgtaa
    20701 atgcgaattt acctggtgac atcgccgtgc gttgggttaa agctgtacct gatgattttc
    20761 atgcccgatt tagcgccacg gctcgccgtt atcgctacat catctacaat catcggctgc
    20821 gcccggcggt attgagtaaa ggggtaaccc atttttacga accgctggac gctgaacgga
    20881 tgcatcgggc tgcgcaatgc ttgctgggcg agaatgattt cacctcgttc cgtgcggtgc
    20941 agtgccagtc ccgaaccccg tggcgcaacg ttatgcatat taatgtcacg cgtcacggtc
    21001 cttatgtggt ggtagatatc aaagcgaatg cctttgtaca tcatatggtc aggaatattg
    21061 tcggcagcct gatggaagtc ggtgcccaca accagccgga gagatggata gcagagttgc
    21121 tggcggcaaa ggacagaacg ctggcggcag caacggcaaa agcggaaggg ctgtatctgg
    21181 tcgcggtgga ttaccctgac cggtatgatc ttccaaaacc gccaatgggc ccgctatttc
    21241 tggcggacta acgagaataa tgctcggaac atttcaggct gtagccaacg tagttacagc
    21301 ctgaaagatg acgagtacaa ggcataggca aatatggacc tgatttattt cctcatcgat
    21361 tttatcctgc acattgacgt gcatctggca gagctggtcg cggagtacgg cgtctgggtt
    21421 tatgccattc tgtttttgat tctgttctgc gaaacggggc tggtggtaac gccattcctg
    21481 cctggtgatt cgctgctgtt tgttgctggt gcattggcgt cgctggaaac taacgatctc
    21541 aatgtccata tgatggtggt gcttatgttg attgccgcta ttgtcggtga tgcagtgaac
    21601 tataccattg gacggttatt tggtgacaaa ctgttcagta acccggattc gaaaatcttt
    21661 cgtcgcagtt atcttgataa gactcatcag ttttacgaga gacatggcgg caaaacgatt
    21721 attctcgccc gttttgtacc gatcgtcaga acgttcgcgc cgtttgttgc gggaatgggc
    21781 cacatgtcgt accgtcattt cgccgcttat aacgtgatcg gcgcactgtt gtgggtactg
    21841 atttttacct acgcgggtta tttcttcggc acgctcccct tcattcagag caaccttaag
    21901 ctgatgattg ttggcattat ttttgtttca atattgcctg gggttttcga atttattcgt
    21961 cacaaacgcg ctgcggcacg cgccgcaaaa taaaaagtaa ctccgcggtt cgaccacttt
    22021 tttatccaaa gtttcgggct gttatgtttt aatgtgcaac attcatggtc tgttgggggc
    22081 aaaaatggca ttatgcgtcc ccaaagataa aactggcatc gaaccaggtt cagacagaaa
    22141 ggtccctaat gagctggatt gaacgaatta aaagcaacat tactcccacc cgcaaggcga
    22201 gcattcctga aggggtgtgg actaagtgtg atagctgcgg tcaggtttta taccgcgctg
    22261 agctggaacg taatcttgag gtctgtccga agtgtgacca tcacatgcgt atgacagcgc
    22321 gtaatcgcct gcatagcctg ttagatgaag gaagccttgt ggagctgggt agcgagcttg
    22381 agccgaaaga tgtgctgaag tttcgtgact ccaagaagta taaagaccgt ctggcttctg
    22441 cgcagaaaga aaccggcgaa aaagatgcgc tggtggtgat gaaaggcact ctgtatggaa
    22501 tgccggttgt cgctgcggca ttcgagttcg cctttatggg cggttcaatg gggtctgttg
    22561 tgggtgcacg tttcgtgcgt gccgttgagc aggcgctgga agataactgc ccgctgatct
    22621 gcttctccgc ctctggtggc gcacgtatgc aggaagcact gatgtcgctg atgcagatgg
    22681 cgaaaacctc tgcggcactg gcaaaaatgc aggagcgcgg cttgccgtac atctccgtgc
    22741 tgaccgaccc gacgatgggc ggtgtttctg caagtttcgc catgctgggc gatctcaaca
    22801 tcgctgaacc gaaagcgtta attggctttg ccggtccgcg tgttatcgaa cagaccgttc
    22861 gcgaaaaact gccgccagga ttccagcgca gtgaattcct gatcgagaaa ggcgccatcg
    22921 acatgatcgt ccatcgtccg gaaatgcgcc tgaaactggc gagcattctg gcgaagttga
    22981 tgaatctgcc agcgccgaat cctgaagcgc cgcgtgaagg cgtagtggta cccccggtac
    23041 cggatcagga acctgaggcc tgataactga taagggcagg gccactggct ctgccctttt
    23101 gctattctca ccgtaacgaa tcagcggata ccatgattat caaacgcact cctcaagccg
    23161 cgtcgcctct ggcttcgtgg ctttcttatc tggaaaacct gcacagtaaa actatcgatc
    23221 tcggccttga gcgcgtgagc caggtcgcgg cgcgtcttgg cgtcctgaaa ccagcgccat
    23281 ttgtgtttac cgttgcgggt acgaatggca aaggcaccac ctgccgtacg ctggagtcga
    23341 ttctgatggc ggcagggtac aaagtgggcg tctacagttc accgcatctg gtgcgttata
    23401 ccgagcgcgt acgtgtgcag gggcaggaat tgccggaatc ggcccacacc gcctcttttg
    23461 cggagattga atcggcacgc ggtgatattt ccctgaccta tttcgagtac ggtacgctgt
    23521 cggcgttgtg gctgttcaaa caggcacaac ttgacgtagt gattctggaa gtagggctgg
    23581 gcggtcgtct ggacgcaacc aatattgtcg atgccgatgt ggctgtagta accagcattg
    23641 cgctggatca taccgactgg ctgggaccag atcgcgaaag tattggtcgc gagaaagcag
    23701 gcatctttcg cagcgcaaaa ccggcaattg tcggtgagcc ggaaatgcct tctaccattg
    23761 ctgatgtggc gcaggaaaaa ggtgcactgt tacaacgtcg gggcgttgag tggaactatt
    23821 ccgtcaccga tcatgactgg gcgtttagcg atgctcacgg cacgctggaa aatctgccgt
    23881 tgccgcttgt cccgcaaccg aatgccgcaa cagcgctggc ggcactgcgt gccagcgggc
    23941 tggaagtcag tgaaaatgcc attcgcgacg ggattgccag cgcaattttg ccgggacgtt
    24001 tccagattgt gagcgagtcg ccacgcgtta tttttgatgt cgcgcataat ccacatgcgg
    24061 cggaatatct caccgggcgt atgaaagcgc taccgaaaaa cgggcgcgtg ctggcggtta
    24121 tcggtatgct acatgataaa gatattgccg gaactctggc ctggttgaaa agcgtggttg
    24181 atgactggta ttgtgcgcca ctggaagggc cgcgcggtgc cacggcagaa caactgcttg
    24241 agcatttggg taacggcaaa tcatttgata gcgttgcgca ggcatgggat gccgcaatgg
    24301 cggacgctaa agcggaagac accgtgctgg tgtgtggttc tttccacacg gtcgcacatg
    24361 tcatggaagt gattgacgcg aggagaagcg gtggcaagta agtttcagaa tcggttagtg
    24421 ggcacgatcg tgctggtggc gctgggggtg attgtacttc cagggctgct ggacgggcag
    24481 aaaaaacatt atcaggatga gtttgcggct atcccgctgg ttccaaaagc gggcgatcgc
    24541 gatgagcctg atatgatgcc agccgccacc caggcgttgc cgacgcagcc gccggaaggc
    24601 gcagcggaag aggtgcgggc aggtgatgcc gcagcaccgt cgctcgatcc ggccactatt
    24661 gcagccaata acaccgagtt tgaaccggaa cctgcaccgg tcgccccacc gaagccgaaa
    24721 ccggtggagc cgcctaaacc gaaggttgaa gcaccacctg cgccgaagcc agaaccgaag
    24781 ccggtcgtgg aagaaaaagc agcgccaacg ggtaaagcct atgttgtgca actgggtgcg
    24841 ctgaaaaatg ccgataaagt gaatgagatt gtcggtaagc tgcgcggtgc cggttatcgg
    24901 gtttatacgt cgccatccac gccagtgcag ggtaaaatta cccgtattct ggttgggccg
    24961 gatgcctcga aagataagct gaaaggttcg ctgggtgagt tgaagcaact ttctggctta
    25021 agtggcgtgg taatgggcta tacgccgaat taatacagtc ttgcctgatg cgacgctggc
    25081 gcgtcttatc aggcctgggc agtgttagaa ccgtaggtcg gataaggcgt ttacgccgca
    25141 tccgacacgc attgcacgat gccgcaaagg cataaaaagt cgatggcgtt gaatattttt
    25201 tcagcgccat ttttattgat gcgtgggaag gaaatcccta cgcaaacgtt ttctttttct
    25261 gttagaatgc gccccgaaca ggatgacagg gcgtaaaatc gtgggacaca tatggtctgg
    25321 attgattacg ccataatcgc ggtgattgct ttttcctctc tggttagcct gatccgcggc
    25381 tttgttcgtg aagcgttatc gctggtgaca tggggttgtg ctttctttgt tgccagtcat
    25441 tactacactt acctgtcagt ctggtttacg ggctttgaag acgaactggt tcgaaatgga
    25501 attgccatcg cggtactgtt tatcgctacc ctgattgttg gtgctatcgt gaacttcgtg
    25561 ataggccagt tggtggagaa aacggggttg tcaggcaccg atcgggtgct gggcgtctgt
    25621 ttcggtgcgt tgcgcggtgt gttgattgtt gccgccattc tcttctttct cgactccttt
    25681 accggggtgt cgaaaagcga agactggagc aaatcacagc tgatcccgca gttcagtttt
    25741 atcatcagat ggttttttga ttatctgcaa agctcgtcaa gtttcttgcc cagagcgtaa
    25801 gtgctctgag atgtggctta acgaggaaaa agacgtatgt gcggtattgt cggtatcgcc
    25861 ggtgttatgc cggttaacca gtcgatttat gatgccttaa cggtgcttca gcatcgcggt
    25921 caggatgccg ccggcatcat caccatagat gccaataact gcttccgttt gcgtaaagcg
    25981 aacgggctgg tgagcgatgt atttgaagct cgccatatgc agcgtttgca gggcaatatg
    26041 ggcattggtc atgtgcgtta ccctacggct ggcagctcca gcgcctctga agcgcagccg
    26101 ttttacgtta actccccgta tggcattacg cttgcccaca acggcaatct gaccaacgct
    26161 cacgagttgc gtaaaaaact gtttgaagaa aaacgccgcc acatcaacac cacttccgac
    26221 tcggaaattc tgcttaatat cttcgctagc gagctggaca acttccgcca ctacccgctg
    26281 gaagccgaca atattttcgc tgccattgct gccacaaacc gcttaatccg cggcgcatat
    26341 gcctgtgtgg cgatgattat cggccacggt atggttgctt tccgcgatcc aaacgggatt
    26401 cgtccgctgg tactgggaaa acgtgatatt gacgagaacc gtacagaata tatggtcgct
    26461 tccgaaagcg tagcgctcga tacgctgggc tttgatttcc tgcgtgacgt cgcgccgggc
    26521 gaagcgattt acatcactga agaagggcag ttgtttaccc gtcaatgtgc tgacaatccg
    26581 gtcagcaatc cgtgcctgtt tgagtatgta tactttgctc gcccggactc gttcatcgac
    26641 aaaatttccg tttacagcgc gcgtgtgaat atgggcacga aactgggcga gaaaattgcc
    26701 cgcgaatggg aagatctgga tatcgacgtg gtgatcccga tcccagaaac ctcgtgtgat
    26761 atcgcgctgg aaattgctcg tattctgggc aaaccgtacc gccagggctt cgttaaaaac
    26821 cgctatgttg gccgcacctt tatcatgccg ggccagcagc tgcgtcgtaa gtccgtgcgc
    26881 cgtaaactga atgccaaccg cgccgagttc cgcgataaaa acgtcctgct ggtcgacgac
    26941 tccatcgtcc gtggcaccac ttctgagcag attatcgaga tggcacgcga agccggagcg
    27001 aagaaagtgt acctcgcttc tgcggcaccg gaaattcgct tcccgaacgt ttatggtatt
    27061 gatatgccga gcgccacgga actgatcgct cacggtcgcg aagttgatga aattcgccag
    27121 atcatcggtg ctgacgggtt gattttccag gatctgaacg atctgatcga cgccgttcgc
    27181 gctgaaaatc cggatatcca gcagtttgaa tgctcggtgt tcaacggcgt ctacgtcacc
    27241 aaagatgttg atcagggcta cctcgatttc ctcgatacgt tacgtaatga tgacgccaaa
    27301 gcagtgcaac gtcagaacga agtggaaaat ctcgaaatgc ataacgaagg atgatgcctt
    27361 cgctgagggt gccggtctgg caccctgact tgcaactccc gccgaaatac tgcaaagtct
    27421 gcctgcaagt ctgacagggc aactatttat gaaacgactc attgtaggca tcagcggtgc
    27481 cagcggcgcg atttatggcg tgcgcttatt acaggttctg cgcgatgtca cagatatcga
    27541 aacgcatctg gtgatgagcc aggcggcgcg ccagacctta tccctcgaaa cggatttttc
    27601 cctgcgcgaa gtgcaggcat tagctgatgt cacgcacgat gcgcgcgata tttccgccag
    27661 catctcttcc ggttctttcc agacgctggg gatggtgatt ttaccctgtt caatcaaaac
    27721 cctttccggc attgtccata gctatactga tggcttactg acccgtgcgg cagatgtggt
    27781 gctgaaagag cgtcgcccgt tggtgctctg cgtgcgtgaa acaccattgc acttaggcca
    27841 tctgcgttta atgactcagg cggcagaaat cggtgcggtg attatgcctc ccgttccggc
    27901 gttttatcat cgcccgcaat cccttgatga tgtgataaat cagacggtta atcgtgttct
    27961 tgaccagttt gcaataaccc ttcctgaaga tctctttgcc cgctggcagg gcgcataact
    28021 ccgctgttgc cctgtttcag ggcaattttg caaccgcgat caaatcctcg acattttgtt
    28081 tctgccattc aatcgaaacg ctgcgattca accgctatac ctgctatctt caacttcagg
    28141 acaataatgc aacgtcttat taacatattt aacgttgaat gttactgttg tcgtcaagat
    28201 ggcataagac ctgcatgaaa gagcctgcaa acacacaaca caatacacaa cataaaaaag
    28261 ccattttcac ttgagggtta tgtatgaaga agtcgattct cgctctgtct ttgttagtcg
    28321 gtctctccgc agcggcttcc agctatgcgg cgctaccgga gacggtacgt atcggaaccg
    28381 ataccaccta tgcgccgttc tcgtcgaaag atgctaaagg tgattttgtt ggctttgata
    28441 tcgatctcgg taacgagatg tgcaaacgga tgcaggtgaa atgtacctgg gttgccagtg
    28501 actttgacgc gctgatcccc tcactgaaag cgaaaaaaat cgacgccatt atttcgtcgc
    28561 tttccattac cgataaacgt cagcaggaga ttgccttctc cgacaagctg tacgccgcag
    28621 attctcgttt gattgctgcc aaaggttcac cgattcagcc aacgctggat tcactgaaag
    28681 gtaaacatgt tggtgtgctg cagggatcaa cccaggaagc ttacgctaac gagacctggc
    28741 gtagcaaagg cgtggatgtg gtggcctatg ccaaccagga tttggtctat tccgatctgg
    28801 ctgcaggacg tctggatgct gcgttacaag atgaagttgc tgccagcgaa ggattcctca
    28861 agcaacctgc tggtaaagct ttcgcctttg ctggctcatc agtaaaagac aaaaaatact
    28921 tcggtgatgg caccggtgta gggctacgta aagatgatgc tgaactgacg gctgccttca
    28981 ataaggcgct tggcgagctg cgtcaggacg gcacctacga caagatggcg aaaaagtatt
    29041 tcgactttaa tgtctacggt gactgatacg tcgctgggaa gctgtacctg atggaatgat
    29101 catcatggtg cacgccaggt ttgttgcact atcgtggtgc attgaaatgc atacttaagc
    29161 atttttaatg aaaaataata cgtctaacgg ggcggaatat tttgccttga tggtcaattt
    29221 tatggcacga taagtgtaac aaacctgtaa atattcccta taaaaagact gtcagttgag
    29281 gacattatga aaaaactggt gctatcgctc tctctggttc tggccttctc cagcgcaact
    29341 gcggcatttg ctgcgattcc gcaaaacatc cgcatcggta ccgacccgac ctatgcgcca
    29401 tttgaatcaa aaaattcaca aggcgaactg gttggcttcg atatcgatct ggcaaaggaa
    29461 ttatgcaaac gcatcaatac gcaatgtacg tttgtcgaaa atccgctgga tgcgttaatc
    29521 ccgtccttaa aagcgaagaa gattgacgcc atcatgtcat cgctttccat tacggaaaaa
    29581 cgtcagcaag aaatagcctt caccgacaaa ctgtacgctg ccgattctcg tttggtggtg
    29641 gcgaaaaatt ctgacattca gccgacagtc gagtcgctga aaggcaaacg ggtaggcgta
    29701 ttgcagggca ccacccagga gacgttcggt aatgaacatt gggcaccaaa aggcattgaa
    29761 atcgtctcgt atcaggggca ggacaacatt tattctgacc tgactgccgg acgtattgat
    29821 gccgcgttcc aggatgaggt cgctgccagc gaaggtttcc tcaaacaacc tgtcggtaaa
    29881 gattacaaat tcggtggccc gtctgttaaa gatgaaaaac tgtttggcgt agggaccggc
    29941 atgggcctgc gtaaagaaga taacgaactg cgcgaagcac tgaacaaagc ctttgccgaa
    30001 atgcgcgctg acggtactta cgagaaatta gcgaaaaagt acttcgattt tgatgtttat
    30061 ggtggctaat cgccatcggt gaaagaaagc ccgctccctt cgggtaaccg gagggagaca
    30121 agaggcggta attcaccaca cacgacagga caggcagcat gttgtatggg ttttcaggtg
    30181 ttattttaca gggtgcgctc gtcacgctgg agctggctat cagctctgta gtgctcgctg
    30241 taatcatcgg tttaattggc gctggcggta agctctcgca aaatcggctt tcggggctga
    30301 ttttcgaagg gtacaccacg ctgattcgtg gcgtgccgga cttagtgttg atgctgctga
    30361 ttttctacgg tttgcagatt gcgctaaaca cggtgacgga ggcgatgggc gtcgggcaga
    30421 ttgatatcga tccgatggtc gctggtatta tcactctcgg ttttatctac ggtgcttatt
    30481 ttaccgaaac gttccgtggt gcttttatgg cagtgccgaa aggacatata gaggcggcga
    30541 cggcgttcgg ttttactcgt gggcaagtgt ttcggcggat catgtttccg gcgatgatgc
    30601 gttacgccct gccaggcatt ggcaacaact ggcaggtgat cctcaaatct accgcactgg
    30661 tttcgttact cggcctggaa gatgtggtca aagccaccca actggcaggc aaaagtacct
    30721 gggaaccgtt ctatttcgcc atcgtctgtg gcgtgattta cctggttttc accactgttt
    30781 ccaatggtgt gctgctgttc cttgagcgcc gctactccgt gggtgtgaag agggctgacc
    30841 tgtgatcgaa atcttacatg aatactggaa accgctgctg tggaccgacg gttatcgctt
    30901 tactggtgtg gcgatcactc tgtggctgct tattttgtcg gtagtgatag gcggagtcct
    30961 ggcgctgttt ctggcgattg gtcgtgtctc cagtaataaa tacatccagt ttccaatctg
    31021 gttatttacc tatatttttc gcggtacgcc gctgtatgtt cagttgctgg tgttctattc
    31081 cggcatgtac acgcttgaga ttgttaaggg aaccgaattc cttaacgctt tcttccgcag
    31141 tggcctgaac tgtaccgtgc tggcgctgac gcttaacacc tgcgcttaca ctaccgagat
    31201 ttttgctggg gcaatccgtt cggttccgca tggggaaatt gaagccgcca gagcctatgg
    31261 cttctcgact tttaaaatgt atcgttgcat tattttgcct tctgcgctgc gtattgcgtt
    31321 accggcatac agcaacgaag tgatcctgat gctgcactct actgcgctgg cgtttacggc
    31381 cactgtgccg gatctgctga aaatagcccg cgatattaac gccgccacgt atcaaccttt
    31441 taccgccttc ggcattgccg cggtgctcta tttaatcatc tcttatgtcc tgatcagcct
    31501 ctttcgcaga gcggaaaaac gctggttgca gcatgtgaaa ccttcttcaa cgcactgaga
    31561 atacgatgtc cgagaataaa ttaaacgtta tcgatttgca caaacgctac ggcgaacatg
    31621 aagtgctgaa aggggtctca ctgcaagcga atgccggaga tgtaataagc atcatcggat
    31681 cgtcgggatc ggggaaaagt acctttctgc gctgcattaa cttcctcgaa aaaccgagtg
    31741 aagggtcgat cgtggtcaat ggccagacga tcaatctggt gcgcgacaaa gacggtcaac
    31801 tcaaagtcgc cgataaaaat caactgcgct tactgcgcac acgcctgacg atggtattcc
    31861 agcacttcaa tctctggagc catatgacgg tgctggaaaa cgtcatggaa gcgccgattc
    31921 aggtgttggg cctgagcaag caggaagcgc gcgagcgggc ggtgaagtat ctggcgaaag
    31981 tcgggataga cgaacgtgcg caggggaaat atccggtgca tctttccggc ggtcagcaac
    32041 agcgtgtttc tatcgcgcgg gcgctggcga tggaaccgga agttttactg tttgatgaac
    32101 ctacctcggc gctcgatcct gaactggtag gcgaagtgtt acgtattatg cagcaactgg
    32161 cagaagaggg gaaaaccatg gtggtagtga ctcacgaaat gggctttgct cgccatgttt
    32221 ctactcacgt cattttcctc catcagggga aaatagaaga agagggcgcg ccggagcagt
    32281 tatttggcaa cccgcaaagc cctcgtctgc aacagttcct taagggatcg ctgaaataat
    32341 ttcctctccg gtggctaacg ctgccggagt attttcatgg ggaatttgcg agtcagcttc
    32401 cgctgaaatg taaaagttgc agtccgctaa aaatacttct gaattcgctg tccaaagata
    32461 aaaactgcct gctgcattgg gtgttaatcg tggttattgt cgcgtcatct taaatttgag
    32521 gggtgacgga acgccatgac aatatcgaca acttccacgc cgcatgatgc ggtatttaaa
    32581 tcttttttac gccatccag
//
LOCUS       DAZPJR010000030        32587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPJR010000030 DAZPJR010000000
VERSION     DAZPJR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.031"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            520..759
                     /locus_tag="ACHVB7_001314"
     CDS             520..759
                     /locus_tag="ACHVB7_001314"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1913345.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(947..1924)
                     /gene="intI2"
                     /locus_tag="ACHVB7_001315"
                     /pseudo
     CDS             complement(947..1924)
                     /gene="intI2"
                     /locus_tag="ACHVB7_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2256..2729
                     /gene="dfrA1"
                     /locus_tag="ACHVB7_001316"
     CDS             2256..2729
                     /gene="dfrA1"
                     /locus_tag="ACHVB7_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1913346.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2824..3348
                     /gene="sat2"
                     /locus_tag="ACHVB7_001317"
     CDS             2824..3348
                     /gene="sat2"
                     /locus_tag="ACHVB7_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1913347.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3417..3914
                     /locus_tag="ACHVB7_001318"
     CDS             3417..3914
                     /locus_tag="ACHVB7_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913348.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3975..4346
                     /locus_tag="ACHVB7_001319"
     CDS             3975..4346
                     /locus_tag="ACHVB7_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913349.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4756..5133
                     /locus_tag="ACHVB7_001320"
     CDS             4756..5133
                     /locus_tag="ACHVB7_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913350.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5156..5308
                     /locus_tag="ACHVB7_001321"
                     /pseudo
     CDS             5156..5308
                     /locus_tag="ACHVB7_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5364..6980)
                     /locus_tag="ACHVB7_001322"
     CDS             complement(5364..6980)
                     /locus_tag="ACHVB7_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR1913351.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(6981..8507)
                     /locus_tag="ACHVB7_001323"
     CDS             complement(6981..8507)
                     /locus_tag="ACHVB7_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR1913352.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8510..10177)
                     /locus_tag="ACHVB7_001324"
     CDS             complement(8510..10177)
                     /locus_tag="ACHVB7_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1913353.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10174..12282)
                     /locus_tag="ACHVB7_001325"
     CDS             complement(10174..12282)
                     /locus_tag="ACHVB7_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1913354.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12269..13090)
                     /locus_tag="ACHVB7_001326"
     CDS             complement(12269..13090)
                     /locus_tag="ACHVB7_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR1913355.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="ACHVB7_001327"
     CDS             complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="ACHVB7_001327"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR1913356.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="ACHVB7_001328"
     CDS             complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="ACHVB7_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR1913357.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(16962..17381)
                     /locus_tag="ACHVB7_001329"
     CDS             complement(16962..17381)
                     /locus_tag="ACHVB7_001329"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR1913358.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="ACHVB7_001330"
     CDS             complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="ACHVB7_001330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR1913359.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="ACHVB7_001331"
     CDS             complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="ACHVB7_001331"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR1913360.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="ACHVB7_001332"
     CDS             complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="ACHVB7_001332"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR1913361.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="ACHVB7_001333"
     CDS             complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="ACHVB7_001333"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR1913362.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="ACHVB7_001334"
     CDS             complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="ACHVB7_001334"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR1913363.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="ACHVB7_001335"
     CDS             complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="ACHVB7_001335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR1913364.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="ACHVB7_001336"
     CDS             complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="ACHVB7_001336"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR1913365.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="ACHVB7_001337"
     CDS             complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="ACHVB7_001337"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR1913366.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(24466..25089)
                     /gene="rsmG"
                     /locus_tag="ACHVB7_001338"
     CDS             complement(24466..25089)
                     /gene="rsmG"
                     /locus_tag="ACHVB7_001338"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR1913367.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(25153..27042)
                     /gene="mnmG"
                     /locus_tag="ACHVB7_001339"
     CDS             complement(25153..27042)
                     /gene="mnmG"
                     /locus_tag="ACHVB7_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR1913368.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(27421..27864)
                     /gene="mioC"
                     /locus_tag="ACHVB7_001340"
     CDS             complement(27421..27864)
                     /gene="mioC"
                     /locus_tag="ACHVB7_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR1913369.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(27954..28412)
                     /gene="asnC"
                     /locus_tag="ACHVB7_001341"
     CDS             complement(27954..28412)
                     /gene="asnC"
                     /locus_tag="ACHVB7_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR1913370.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            28564..29556
                     /gene="asnA"
                     /locus_tag="ACHVB7_001342"
     CDS             28564..29556
                     /gene="asnA"
                     /locus_tag="ACHVB7_001342"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR1913371.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(29561..31012)
                     /gene="viaA"
                     /locus_tag="ACHVB7_001343"
     CDS             complement(29561..31012)
                     /gene="viaA"
                     /locus_tag="ACHVB7_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR1913372.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(31006..32502)
                     /gene="ravA"
                     /locus_tag="ACHVB7_001344"
     CDS             complement(31006..32502)
                     /gene="ravA"
                     /locus_tag="ACHVB7_001344"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR1913373.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         8313 a         8237 c         7556 g         8481 t
ORIGIN      
        1 tcggcagccc tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt
       61 taaggattat ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct
      121 aatacgaaaa ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttaact
      181 aatctagcta acctgccgct cagcttagta cgatcctata tccaactgat cataatcctt
      241 tcatcagtat cccaaattaa atccccctcc tagactgcct caccaacact ttacagtgat
      301 cccgtatttc cggacaggtg attgattttt ttcgcttgta caggtgttaa gccacagtta
      361 tcttgatgtg gacattatgc attgtaatag cccattaaca tgcgctaaga gcataaatta
      421 atatacagta acaagcatta cgaggtgcta atgtttggaa agcagcctct tccaactaca
      481 ataataagtt aactatcctc cacccacaaa taattacaaa tgaccccttt ccgctataac
      541 tccgatctca ccagcggctc gctacaaacc cgagagtgtc gcattattac tggcttgtta
      601 ctccaagagc ttgatgaagc tgcctgggat aaagccatgt ataaggaaaa tgtgcttcag
      661 aaacgcacgc aatctacggt aagacgtatt tcttcagcgc ttagaaaacg cctagaacac
      721 ctaagctctg acttttgggc ttttgcgttt ttatgttagg cattgtctgg caactggagg
      781 aagtgtaatg accatgaatg gtcactgtga ttgacagtat cttgcttaaa cttatctgaa
      841 caatggtaga actggctcat atttatcccc tctatgattg tttatctttt tcaccataaa
      901 aagtatcagg ggctactcag ggctacataa agatatttat ctacttttac tgattgataa
      961 gtagcatcag tccatccgca ggactggtgg tgccggcaaa atgctgaccc aacacatgcg
     1021 tatagatttg cgtggtctta acatcgttat gccctaagag ttcttgcaca gtgcgaatat
     1081 cacgccccgc ttgtaataga tgcgtagcaa acgagtgacg aaatgtatga caagtgacac
     1141 gcttgctaac gatgcctgct ttttgtacgg ctgccttcaa tgcctttcgc gcaacggagt
     1201 catgcagatg atggcggcat aatttgccgt tatacgggtg gttgcagagc gtgctggagg
     1261 gaaagacaaa catccacgcc gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa
     1321 aaggcagcga tggccctacg ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct
     1381 caatgagtta ttttattgct gggattaggc gcgtgggcag taggctgttt ctgcttttcc
     1441 cacccttacc gtcatgcaca gtgatgcagc cattatcaaa atcaaaatct ttaacccgca
     1501 aacgcaagca ttcattaatg cgcaaacctg caccatacag cagcgtaaaa ataacttggt
     1561 tgcgagtatc cataacctgc aaaatgcgtt gcacttcatt tgcagagata acagagggta
     1621 gccgtctagg cttgcttgca gggatataat caatatcgcc caacggctgt tgtaaaaacc
     1681 tgttgtacaa aaaagctagg gcatttaaag cgattttctg cgtgtttatg gctacatgtc
     1741 tgctgtttgc taagctggat aaaaacagcc tgacctcttc actgcccatg gtctgaggat
     1801 gacgtttttt gtgaaacaga ataaaacgct taatccagtg caggtaagtt ttttcagttt
     1861 tcagcgcata acctttttgt cgcatatccg tgcgtataga atttaaaaat ggactgttag
     1921 acataaaacg ctccttgtct tgcaactgtc tgcctataca gcctattcta gctgggattt
     1981 aaaaaagtgc ctgtttttta cgcctagaga tgcttgttta ccggtagagt tttaatttaa
     2041 tgctaaataa attaaaatgt tatgagttct ttgggtgaga taatgtgcat cgtgcaagca
     2101 ggatagacgg catgcacgat ttgtaataac agagtgtctt gtatttttaa agaaagtcta
     2161 tttaatacaa gtgattatat taattaacgg taagcatcag cgggtgacaa aacgagcatg
     2221 cttactaata aaatgttaac ctctgaggaa gaattgtgaa actatcacta atggtagcta
     2281 tatcgaagaa tggagttatc gggaatggcc ctgatattcc atggagtgcc aaaggtgaac
     2341 agctcctgtt taaagctatt acctataacc aatggctgtt ggttggacgc aagacttttg
     2401 aatcaatggg agcattaccc aaccgaaagt atgcggtcgt aacacgttca agttttacat
     2461 ctgacaatga gaacgtattg atctttccat caattaaaga tgctttaacc aacctaaaga
     2521 aaataacgga tcatgtcatt gtttcaggtg gtggggagat atacaaaagc ctgatcgatc
     2581 aagtagatac actacatata tctagaatag acatcgagcc ggaaggtgat gtttactttc
     2641 ctgaaatccc cagcaatttt aggccagttt ttacccaaga cttcgcctct aacataaatt
     2701 atagttacca aatctggcaa aagggttaac aagtggcagc aacggattcg caaacctgtc
     2761 acgccttttg taccaaaagc cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt
     2821 catatgaaga tttcggtgat ccctgagcag gtggcggaaa cattggatgc tgagaaccat
     2881 ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga actatctacc
     2941 agaagtgtga gcccctaccg gaaggattac atctcggatg atgactctga tgaagactct
     3001 gcttgctatg gcgcattcat cgaccaagag cttgtcggga agattgaact caactcaaca
     3061 tggaacgatc tagcctctat cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga
     3121 gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac taagcagaca gctccttggc
     3181 atacgattag agacacaaac gaacaatgta cctgcctgca atttgtacgc aaaatgtggc
     3241 tttactctcg gcggcattga cctgttcacg tataaaacta gacctcaagt ctcgaacgaa
     3301 acagcgatgt actggtactg gttctcggga gcacaggatg acgcctaaca attcattcaa
     3361 gccgacaccg cttcgcggcg cggcttaatt caggagttag agagctgggg aagactatgc
     3421 gcgatctgtt gaaggtggtt ctaagcctcg tacttgcgat ggcatcgggg caggcacttg
     3481 ctgacctgcc aattgtttta gtggatgaag ctcgtcttcc ctatgactac tccccatcca
     3541 actacgacat ttctccaagc aactacgaca actccataag caattacgac aatagtccat
     3601 caaattacga caactctgag agcaactacg ataatagttc atccaattac gacaatagtc
     3661 gcaacggaaa tcgtaggctt atatatagcg caaatgggtc tcgcactttc gccggctact
     3721 acgtcattgc caacaatggg acaacgaact tcttttccac atctggcaaa aggatgttct
     3781 acaccccaaa aggggggcgc ggcgtctatg gcggcaaaga tgggagcttc tgcggggcat
     3841 tggtcgtcat aaatggccaa ttttcgcttg ccctgacaga taacggcctg aagatcatgt
     3901 atctaagcaa ctagcctgct ctctaataaa atgttaggcc tcaacatcta gtcgcaagct
     3961 gaggggaacc actagtgtca tacgaacctc caagagacgg ttacacaaac gggtacattg
     4021 ttgatgtcat gtatgacaat cgcccaagta agtatccagc tgtgttcaga acgtacgtcc
     4081 gaattccaga ctatgtcgaa ctagataacg cggaggagct ggccgacgca atcattcgag
     4141 aggttgagtt aaaggaggca tggctcacaa gggtcttggg gaactcagcc gatcctgacg
     4201 cttctgtggg caagtgggac cggcgcctag cgcagtgcgg tggtattgat gagatgtcaa
     4261 gtggggtgca ggcaacatac ttcagtccaa acggtcagct ggtcggctta gtggcataca
     4321 ttgatgccga atggatggat gaatagctct gacgttacga ttcaggtgag acaaccagat
     4381 gggcattgcg tacaagctca gttgccaccg atgtggctac gagtctgatc ttctttacct
     4441 aggccaaggt atggcgatgc ttcctgagca aatagtcgga acgtgcacat gctgcgacaa
     4501 tctaacgacg atttctataa atgaaagcta cggagtatgc tcactttgtg gggctaagga
     4561 tcgtgtgata tttacaagct atcagcatga ggcaaggcca agaaatccct gctatctaga
     4621 gagaagtttc aaatatcaat gcccaagatg ccatgccttc agtatggagc ctccggatct
     4681 accggagatg ctttgggact aatgctccgc tttgcctcgg gtatccgttc gacctatgtt
     4741 taagaggaaa cgcacgtgtc tgccgccaaa gctcttgcct atgcactttg tgacaagttg
     4801 gaaagcaaaa aagttgatga agtcgtacga aatcttgttt ctagcggctg gaatattagg
     4861 gaagtggcct gggaagcttc gtcctttgat catgaaatgc cctactttga cacggggcgg
     4921 gacttcgata ttggatttcc tgacacgctg tcatccaaga ttgagttttt gaccgcgaat
     4981 ggatcaatcg tccaagcacg tgctcgcctg cacttcaagc aggctctaat ttttgccaag
     5041 gcgtccaaac atgtcgccgc gctcaagaac tcattggatt actactatgg atcgtctgta
     5101 atcccccctc aaaaccacgg aggctatttg tagaaaattc cattaatatg aactcaaggt
     5161 gttccctacg gacgaatcag cgatgaaggt ggtgtacctg gctatccagg cggcgaccaa
     5221 gaaatggacg atgccgatcc gcaactggaa gccggccatg aaccgtttta tgatcgaatt
     5281 tggtgaccgt ttaaatggcc acctttaact gaccggcaaa agcacttaca caaaagggtt
     5341 tacaggctct ttcagtcatt cctttaatgc gtaaattgct ctctaaccac tctctcagcc
     5401 cacccttcca tatcctctct cgatacttgc agctccccca acccctttgg atgattcacc
     5461 cctctatgcc cgtcttgtcc atataattga tcaaacaaac tatttggcca attcaatgag
     5521 ctcttcacca ctccacgccg tatcttttca aaatcattcc gccatgtttc gctatcaacg
     5581 ccactcaaca ctttggtaga aagcattttc accccatccg atgcatccac ctctagcaat
     5641 acaaattcac tgccattacg ttgcacccca acggctttaa tcactcttgg tgagccatct
     5701 ttacacagat gtaatcggct acgcccaact tttggcaaaa ccaaggtttc ttcaaaaagc
     5761 acccgacatg caaatttagt ttttagaatt gaaagtagct catcaaaagc agcaaatcga
     5821 ttagcaaaaa tagagttgta attggttgca tcctgcttcc cacccacatc tgccgctgct
     5881 aggacaccac ctaagtgtgg ctcatcagta ctaaccaaat tactattttc acttgttgtt
     5941 gcttcttctt tttctaaaat agtttggcta ctttttcttg ccctgcgaga tttttcaatt
     6001 cgactaggtc tactaaaact tatccatgtc ggctcagcct ctatgactcg tgtgtctgtg
     6061 tctatattgg ctgtttcctc atcgtccaac tgtagatgct catcatcaac aactgactct
     6121 gttgaaaccg ctatgtgctg cgtactacca tcacctgcct ccttttcctg aaaagaggca
     6181 tggctaatag ctgttgtgct aggaagcata gcgttgattt ctaaacctac tatttcctca
     6241 actaaataat ccttatcctc gttagaagaa cgtcctttta catgtaattt ccaaccctgc
     6301 atgggcggag ggtcaaagct aaagcaccag ctttcaacgc cgttcttaat ttctctattt
     6361 tgttgatagt gcctaaaaat actttcatac gaatccataa catcttgatc cgaaaacaac
     6421 caaaccaaaa gctgcactac ggccgactgc tctaacgccc ctttaggaaa cgatgaactg
     6481 ggtaagatcc ttatctctaa atgatctcgc tcaacctcat actgaacgtc gaactcttgc
     6541 tgtaaagcgg tactgctcaa acagcttcga cagaaatagg agttaattaa aaataatgac
     6601 cgagctagct ctaactgtgg gatgtgataa cgagtcttgc tatatggtgt ttcagcttcg
     6661 taaataaatg aactctgcgc tccatccttg ttgcgtacgg aagcaaagga cgagagatta
     6721 ggaaaatcga ttattttggc tcgttgaaga tccgatgctt caaactcaat cacacgatcc
     6781 gccttattta tttgctttgt tgtactgtta accaccttag ctcgacttaa taaaggcaaa
     6841 tgagtaaatc gagtccattt ccgttcttgc attggattaa accaaacaaa aatacgccac
     6901 tccttatgac ccgagttacg gaataaatga ccaatatgta caacctgcac attgtcatta
     6961 aatgtagcta gcctaaccac tcactcttcc ccataaacca tttccaagaa tctttgtgct
     7021 tcctcagtta tccgctcttt agataacgtt gcacttctta ataatcgcca gcgcctaagc
     7081 tcaacatctt cctgtttaag cttaataaaa gcttggctaa tccgtctaat ttgataatct
     7141 tccacactct ctgagtaacg ctttaagcaa agcgctacca aaggcagttt ctgtagattt
     7201 tttgcaagag aggttccgtt aggagtttgc tttaacagcc agctcgatgt cgctcttggg
     7261 tgatcaaggc tactatctag acgctttatg attcttaata gttgtcgtac agcaattcga
     7321 tctctttggt tccaatcaac tctaggggcg ggtgccagac gctcttgttg atgctgttga
     7381 ttccagtgaa ctagccaatc cctgtcatgt cggtaaagcc aagcgtatag caccccaccc
     7441 tctaaagact gtcttgccgc cttaattcct tggtatttat gcactagttg ctgccagtct
     7501 ttatgcttaa cagataaatc ttcagagtta ggttgcacag actggctaac aggtcttgtc
     7561 gttatagagt gctcagtaag agcacttgcc tgctgtagcg cttctataac cgttagtttt
     7621 ggcaataagg cttgccacac aatactatgc tgtaaataac taaaggcttt tctatgctta
     7681 cggaatatac ttttcagcca acacgtgtcc ttgttctctg ccaactttaa atccagtttc
     7741 tctagtgcct catcactaaa agtctgcctc actctctccg ccaccaagtc atgacgaatg
     7801 tgcttgcttt tggttagccc tagatcctgc gctaagcgct gataaaacag cgtccactgc
     7861 tcaaggcttg gggaaagctc ttgcgctcgt ggagcatcta acagaggggc tatataagca
     7921 gctagtgctg ttaattgaga tagggagtct ttggggtagt ctgaaagcag ctcagtatga
     7981 cccaaagccc aaaattgatg tcggtgatca tctacagctc tatcaaagaa gactaaagca
     8041 ccgtgttttg gacaatatgg caaagcgggc aaataccaat ctcgttgcca aaaggcttcc
     8101 ccatacctat ttagctgaag agcaacgcaa tcagggcagt atctaaagcg gttatcgctc
     8161 ttaactctag aagcagcgac tcctagcatt aaatgcaccg caccttgcgc ttggtactcc
     8221 attaaccgaa tagcttcgtc tcggcgctcc ttgcctacaa acggagcata taaagggaat
     8281 aaggtatgct cataaataag ctgctgaaca gcgtaacgtc ctgtttgatg tagatgtctt
     8341 gctatcacac ctaaatgcga gggcagacct aaggtagcga ccaccttgcg gttgccatac
     8401 acctcatcca acagctgctt aggactaaca atcccttgat aaacgcctgc ccgtgcaata
     8461 gtgctataaa tcagctcatt cgagtacgga acaggaaagt ttctcatggc taacctgctt
     8521 gtttaaataa gctcgccata tccactatta cccctttccc ttttaaccgt tcatgcatgg
     8581 ttttttcagg ttggcgttgt gaatagatat aacgtaaatc cgtatcaggc aagctatccc
     8641 agtcgctggg cttgactacc tttatagccg aaaccttttt actcttggag ggcttttcta
     8701 gttctgacac taccgtttcg ccttccatca accactgcaa aacaagaggc agtaactttt
     8761 gtcttgtcat cgttggattc tggctaaacg cttttttaat agtgggaatt aacaggcttg
     8821 aatcgtaatc ctctttcagc atcagatata aatgacgctg atcttcggta tctaactctt
     8881 gaagggcttt ttcttctggt gtttgttctt gtatcgctgc gatatctagc tgaagttgga
     8941 ttaaccgttt atcaatctcg ggaacgacta gatcagaata acgagcaatg cgttctggga
     9001 tacccgagcg taatgcctct agcatggggt gcacaggctt taactcatct tgatacactt
     9061 gccgcaataa accagcggta atacgctcat tgcctaaagc tagcgcacgg agctgagcga
     9121 gtacaaaaag ttttactaca atgtccatca ctccttggct tagctcatac cacacatcac
     9181 ggacctcatc cgataacagc gcatctttgc gttgtaaaag ctgtaattgc caaagattat
     9241 ccgtaaaagc gatccactct tgattgggct ttccacgttg cgtttgttgt ataggatccc
     9301 agaatatagc tccaaaccct gcccctctac gtgcagaccg caaatcagcc tcaaaaatct
     9361 ctcgtgcttt aggggtacca atcaacatca ctggtacgcc aataatattc accatcgtca
     9421 caaaaaagtt cagcatctct tgagatccac ccgaacgaga gcggcttaaa tgctgaattt
     9481 catcaataac caacaaccct aaagcatgtg cattggctat ttgcgacatc aaagccaaca
     9541 tggtttctat accatgacgt tttaagccat aacgacgctc atagttcgag cccaaggctc
     9601 gatccaacgc tctgaaaaaa ttcaagcaga tttcttttag cgaaccatta tgcgagcagt
     9661 ctattttcaa atacaccacc tgctctacat tgagttcacg atggtaaatc acctgaggat
     9721 acgtggctag aatacgatga agagaggtcg tcttcccact accagaacaa ccaattaaca
     9781 ataagctttg tgccgtagat cgtgcctcct caaagcgaaa tgtctccaac tctcccgttt
     9841 gaacacgctc ataaccattt tgtaaatgct tttgtaaatc tcctgtttta ggatttctgc
     9901 ctacgtagcc acctcgaatc atgaccgaaa tacgctcact tagtagcaaa tgcgtaccta
     9961 atggctgaaa atagtcatct ggaatacgac aaatggtatg agctctgata acacgggact
    10021 tttgcaagtc agaggaagta agctgtaaag aggatttcag tgatgcagca ctattcactg
    10081 actcttgtaa tggtggtaag gcctcgataa aaggattatc acgataagcc tctacccccg
    10141 tatcacgata aactgcttga atccgggtag cactcatgac tcatcctttt ctggtggatc
    10201 ctgaaataat tcaggcacgt atgtgggtag gctgtaatct tcttgatcat ccgcttccac
    10261 tgcgttgaaa ggaataactt tagcctcatc acctgaagag cttggcttca aatgctccgc
    10321 acgtttttta cgctccgagg tcacggcttc ttttttatta gttttaatct gcttaatgcg
    10381 tgttgatttg ggctcagtag tactgggcgt taacttattc gctttctgaa tggtttgctg
    10441 aataaacgcc tcaagctccc tgcgtttagt taactcatcc tgcttcgcat tggctttatt
    10501 gtgtttttct tgtgcttgta tatcccaaac ctcccaaaat gagagacctt taaactgccg
    10561 actacgttcc gtcagattac agcgccaaaa tacacggctg ccaacttgcg gaaacaaata
    10621 aatcgtatca accagcactg ggtcataagc cgcttctaaa tgttgaggtc tagctatatc
    10681 agtgctccgc tgcaaccaac cctcacgcag aatctctgac cccgagtaat acaaacccca
    10741 caaattaacg ccaaatgaag aaatagagac ctttcggcga ggcagtaacg ctactcgcaa
    10801 ctgctcttgc tccacagccc ttaaactacc tgtacgatgc tgcatacccc attgccatag
    10861 ctggacagga atagacggta aatctgtagg aaaatcagca tctcgatcgt atttatccat
    10921 caccagatgg ttatttctga ataagatcgt acgcaaaata atttgtgtga actcaaatac
    10981 cgacagagat gcatctaacc tatagtctgt ttcaccatgg cttttgatcc gactgccctc
    11041 tacaatgcca ggtgcaaagg acttaaactc ggcttgtagt gttctaaaag tgctttccac
    11101 tatgccttta gcatcgccac gtctaggtgg agcactttcc actcgcacat taaaactaga
    11161 aactaaggct tcgacctgat gactcattaa ttcgccacgg tccgctagca acacatctgg
    11221 caaacctaca cacggccagt ctgagctact aatctcaata tcatgctggg cacaaatggc
    11281 cgttttgtca gagcaagcat ttacaaaagc ctgcatcgcc accacataag acggattttc
    11341 aaagccgata taaaagcccg tgatcatccg actaaacaca tcaatcacaa tgtaaagcgt
    11401 tggtcttcct atgatttttt ggcgatcatg atgatccact aaataaatat cagcaatcgt
    11461 ggcatcaatc tcataacgac tcccagggcc taacgcctga gaagtggctg tactacttaa
    11521 gggtcgtacg tcttttttat atacccctgc tttaactcta gactttaagc gctgagcttt
    11581 agggtattct cgatcataaa aataacgaaa ctgacgtagt gttgggtaat cctcttgggg
    11641 aatgcgagga aaatactgag caaacaagtc cacaaatcgt ctataggcaa cggtggtctt
    11701 tgtacctttt tgatttaaca ggtgcttttc tatggtcaac ctaaaaaggc gttcaatctc
    11761 gggcgttacc ttggttcctt cacccttacc atattctctg gctcggccaa tctttgctgt
    11821 tcctgtcgct gaacgtcttt cccctggtgc accgctgttt ttgtagtcag gaattaatgc
    11881 attaggcgtt tgaccacgct gccagtaacg gcgtaacaac ttataaactg tagccttagt
    11941 aaccttatgt tcttggacca catgctcaac gagttcgctt ctgactttag ggtcgaaacg
    12001 atccttacta ttaataatag gaagaatttt tcgataatcc tcctcgcgct tctggaagct
    12061 gacagaatct acagacggct cttctaaatc aagatgtaca taagggtcat cagttctaac
    12121 taagcggcct tcatctaaat actgcattag caacaactca gccctagctt gtggaactcc
    12181 tttatcagcg cttatttgca tccagacaac ttggccatcc tctatagcca aaatgcgata
    12241 cggatcatta tcaaatagca caacctcatt aatttgccac atagcgcaac tcctccatat
    12301 tcactacttg gctaatacag agatctgcac acttatttgc cctgaaagac ttataaatat
    12361 tgaacttaat aaaaccattt gcggttaagg ctcgtatctc actcaatgtt ttgcctaact
    12421 ccaaatcata agcaatatca acctgcttac agacattgat aatgttttca tctccttttt
    12481 cttgcaggat atgggccaat ggggatagtt gtgctaaaag ctccgcagaa acttcttctg
    12541 ttttcactga ataaagccat tcaatatttt cttttactac gggatttatt tctttatcag
    12601 taaaaatgaa ccaaggaatt tgcttttgct gccaatagcg acgctctagt tctagttttt
    12661 ctaaggtacg ctcgtcttgt aaggctgctg caggtttgac ttgaatagca aactgctcaa
    12721 aaggaccatc tttgcagtcc actaaaaaat cagtagacat aacctgatct acaccacgaa
    12781 taacaggatg cttaatacca ctatctattg caatctgcct ggtatcacta ggtaataagg
    12841 ggaactgctc gcgtatatct agcacgctgc tctcccactc aagactgaga aaaacagcaa
    12901 gctctaagtc agatagcaaa tgatggactc gtcccgtctt atgagaataa atacggtggg
    12961 aacgacctga agaaggaact tcttgtactg ttagccatgg aatatagtct ttaccatgcc
    13021 cttggccacg cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg
    13081 ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt
    13141 tacaactatt ctgtctagtt taagacttta ttgtcatagt ttagatctat tttgttcagt
    13201 ttaagacttt attgtccgcc cacaagccct tttttatcta tgatagtgat tactcaaccg
    13261 taaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc agcgcgacat
    13321 ggtaagccag cagctgcagc ggaacggtgt agaagatcgg tgcaatcacc tcttccacat
    13381 gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc tgatcggcga
    13441 agacatacaa ttggccgcca cgcgcgcgaa cttcttcaat gttggatttc agtttttcca
    13501 gcaattcgtt gttcggtgca acgacgataa ccggcatatc ggcatcaata agcgccagcg
    13561 gaccgtgttt cagttcacca gcagcgtagg cttcagcgtg aatgtaggag atctctttca
    13621 gcttcaatgc gccttccagt gcgattgggt actgatcgcc acggcccagg aacagtgcgt
    13681 gatgtttgtc agagaaatct tctgccagag cttcaatgcg tttgtcctga gatagcatct
    13741 gctcaatacg gctcggcagc gcctgcagac catgcacaat gtcatgttca atggaggcat
    13801 ccagaccttt caggcgagac agcttcgcca ccagcatcaa cagcacagtt aactgagtgg
    13861 tgaatgcttt agtggacgcc acgccgattt ccgtacccgc gttggtcatc agcgccagat
    13921 cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa ccaaggtaac
    13981 ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca gactgtgaca
    14041 aggtgatcat caggctgtta cgacgcacgg cagatttacg atagcggaat tcagaggcga
    14101 tttcgacgtc gcacggaata cctgccagcg attcaaacca gtagcgggaa accataccgg
    14161 agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac agcagttcgt
    14221 cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg
    14281 tgttttttat cgcgttcggc tgttcgtaga tctctttctg catgtagtga cggtaaatac
    14341 ctttatcgcc cgcgtcatat tgcagattgg attcgatatc ctgacgtttt acttccgcgc
    14401 cagttttatc gaagatgttt accgaacggc gagtgatttc cgcaatatcg ccctcttcaa
    14461 ggaagataaa gcgacgggtc accggcaaca gcgccagctg gtcagaagcg ataaagtttt
    14521 cgcccatacc caggccaatc accagcggac taccagaacg tgccgccagc agggtatccg
    14581 ggtgacggga gtccatgatc actgtaccgt acgcaccgcg cagctgcggg atagcacgca
    14641 gaacagcctc acgcagagtc ccgccttgtt tcagctccca gttcaccaga tgggcaatca
    14701 cttcggtgtc ggtttcagaa acgaaggtat agccacgcgc ttttaactct tcacgcagcg
    14761 gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa acatgcggat
    14821 gcgcgttagc ttctgaaggt tcaccgtggg tcgcccagcg agtgtgagca ataccggtgc
    14881 cgccatccag aggatgttct tccgctgcct gagccagcat ctggacttta ccgaggcgac
    14941 gcaggcgggt catatgacct tccgcatcaa caacggccag accggcagag tcatatccgc
    15001 ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg
    15061 cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg tcaacctgta
    15121 tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt atagttactg
    15181 cttgtgggag ggagattatg ttatctcctc atcccagatg accggattac ccggccagaa
    15241 ttactttttc tttaccggac gacgccagcc ttctttctga gtctgcggca cacggctgat
    15301 agctaatgca ttttcgccga cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc
    15361 tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc
    15421 gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat
    15481 gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgcccga ctttcgagcc
    15541 tttacccaga cgcgcttttt tcatctcaac gaagttacca acgtgtgcac cttccagcaa
    15601 ctcagcacca ggacgcaaac gagcaaacgg gccaatagta caggccgctg ccagattcgc
    15661 gtcttccaca acggtatacg gactgatttc gcaatcatcg ccaatcacgc tgtttttaat
    15721 cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct cgatgataac
    15781 gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc
    15841 cggatcgcgc agcataacgc ctgctaacag cagtttttca gcctgttcgg actggtaaac
    15901 gcgctccaga cgggagagtt gcaggcggtt attcacacct tctacttcgc ttaaacgttg
    15961 cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat
    16021 gtagtattcg ccctgagcat tattgttggt cagcttcgcc agccagcgtt tcatatctgc
    16081 gccgttagcg atcagaatgc cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc
    16141 atctttgtgc tcaacaatgc cggtaacttt gccattttca cgggtgatac gtccataacc
    16201 ggtcggatca tccagtttca ccgtcagcag accaatgcca ccctgcggtt tagcatcacg
    16261 caggcgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc
    16321 ttcatcatcg gcaaagaaag gtgcggcctg ctgcattgca tgacccgtac ccagctgctc
    16381 tgcctgaagc acccagttaa ggttgtcgtc tttcagcgcc tgtttaagca gatcgccgcc
    16441 gtgaccgtac accaagtgaa cgtgcgctgc gcctaattca ttcgcagcat caatgacatg
    16501 ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg aatacatgcg
    16561 cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca tacgcgtcct
    16621 gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca tttttttcat
    16681 cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga cgaaagtgcc
    16741 attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc ttcatttaag
    16801 cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa acaggggtgt
    16861 tttatatcag caggatctat gtgaacgctc ttcaggacgg atcacgcgta aaaaaaagcc
    16921 agcctgtttc cagactggct tttgtgcttt tcaagccggt gttacatcgc ttttttggtc
    16981 aactcgataa cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc ctgagcgtaa
    17041 tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc ttccatggct
    17101 cgcgcttcgt cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt cacgttgcca
    17161 ggctgcactt caagaatgcc gccagacaga tagataaact cttcgtgacc gtgctgtttc
    17221 acgatgcgaa tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc agggtagatc
    17281 cccagttcac cttcgctacc cgttacctgg attttctcga ccagaccaga gaacatttgt
    17341 tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca tatcaccctc cgattaaggc
    17401 gttaaagttt tttggctttt tccacagctt cttcgatgga accgaccatg tagaacgcct
    17461 gctccggcag gtgatcgtat tcgccttcca tgatgccttt aaagccacgg atggtgtctt
    17521 tcagggagac gtatttaccc ggagaaccgg tgaatacttc tgccacgaag aacggctggg
    17581 acaggaagcg ctggatctta cgagcacgcg ctaccaccag tttgtcttct tcagacagtt
    17641 catccatacc caagatggcg atgatgtctt tcagttcctg ataacgttgc aggatggact
    17701 gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc agctgacggc
    17761 tggtggagtc cagcgggtca acggccgggt agatacccag agacgcgatc tgacggctca
    17821 gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca gtcaagtcat
    17881 ccgcaggtac gtataccgcc tgtacggagg tgatagaacc agttttggtg gaggtgatac
    17941 gttcctgcag aacgcccatc tcttccgcca gggtcggctg ataacctacc gctgaaggca
    18001 tacggcccag cagtgcggat acttccgtac cggccagggt gtaacgatag atgttgtcaa
    18061 cgaacagcag aacgtcacga ccttcgtcac ggaatttctc agccatggtc agaccggtca
    18121 gagcaacgcg cagacggttt cccggcggct cgttcatctg gccatacacc agggatactt
    18181 tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc gttaccctca cgagtacgtt
    18241 cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta cgaatgagct
    18301 ccatcatgtt tacggtttta cctacacccg caccaccgaa cagaccaact ttaccgccct
    18361 tagcgaacgg acacatcagg tcgataactt tgataccggt ttccagcagt tcctgagagt
    18421 ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct tcttcaccga
    18481 tctcgccttt catgtcgacc ggttcaccca gtacgttcat gatacggccc agagtcgctt
    18541 tacctaccgg gacttcgatc gggtgttcga ggtcttttac atccagaccg cgacgcagac
    18601 cgtcggagga acccattgcg atggtacgca cgataccgcc gccgagctgc tgctgaactt
    18661 ccagcaccag acgctcatta ccattttgca cctcaagagc atcgtacacg cgcggtacgg
    18721 catcctgagg gaattcgacg tcaactacgg cgccgattac ctggacaatc tttccagtag
    18781 ccatcttaaa tcctctacga aataacctgt ttaaaccgcg gcggcccccg agacgatctc
    18841 ggtgagttcc tgagtaatgc tggcctgacg agctttgttg tataccaact gcagctcttt
    18901 aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc accatacggg cggcctgctc
    18961 gctggccagg ttttcaacca cgccctgata aacctgagat tcgacataac gacgcagcag
    19021 ggtatccagc aacgccttcg gatcgggttc gtacaggtaa tcccaggatt tatgtttcag
    19081 atcatcatca tctgatgccg gtaacggcag cagctggctg atggtcggaa cctgagacat
    19141 ggtgttaata aatttgttgc tgacaatgta aagcttgtcc agacggcctt cgtcgtaagc
    19201 ctgcaacatc acttttaccg gaccgatcag ttcggacagg gaagggttat cccccatgcc
    19261 ggtgacctgg gcaacaacat tgccgcccac ggagttgaag aacgacacgc ctttcgagcc
    19321 gatcattgcg aggtcgcatt gaacgctttt gtcggtccag gtcttcattt ccgccagtag
    19381 ttttttgaac aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg acaccaccag
    19441 gtagcccacg cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt ccagattacc
    19501 gtgtgcaagg tgaccaatca ctttgcgcat ggtttctgca taaggacggc tggccgccat
    19561 gcgatcctgc gatttacgca ttttggaagc ggcgaccatc tccatcgctt tagtgatctt
    19621 ttgcgtgttc tggacgcttg cgatcttact acgtatctct tttgcgccgg ccatgagctt
    19681 ctcctcaatg ccttgcggcc tgccctaagg caagccgcca gacgttacca ggattgggtt
    19741 gctttgaagg aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt gtagccaccg
    19801 gtctggttga tctcttgcat caacggagcg tgatcacggt cgacgtaagc cagcagagcg
    19861 gcttcgaagc tgccaatttt cgacagttca acatccgcca ggtaaccacg ttctgctgcg
    19921 aacagaacca gagactgctg cgcaacggac atcggcgcat actgtttctg tttcagcagt
    19981 tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag gtcggatgca
    20041 aactgagaga acgctgccag ttcacgatac tgtgccagag cggtacggat accaccggac
    20101 agttttttca tgatcttggt ctgtgctgca ccaccaacac gggatacgga aatacccggg
    20161 ttaaccgcag gacgaatacc ggcgttgaac aggttggttt ccaggaagat ctgaccatcg
    20221 gtaatggaga ttacgttggt cggaacgaac gcagaaacgt cacccgcctg agtttcgata
    20281 atcggcagtg cggtcagaga accggttttc cctttcactt cacctttggt gaaggcttca
    20341 acgtattcgg cgttaacacg tgcagcacgc tccagcagac gagagtggag gtagaaaacg
    20401 tcgcccggga atgcttcacg tcctggcgga cgacggagca gcagggagat ctgacggtaa
    20461 gcaacagcct gtttagacag gtcatcgtaa atgatcagcg catcttcacc gcggtcacgg
    20521 aagtattcgc ccattgcgca accggcatac ggtgccaggt attgcagtgc agcggattca
    20581 gacgcggttg ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc cagtttacgt
    20641 accacgttag aaatggtgga cgctttctgg ccgatagcga catagataca tttgataccg
    20701 gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg ttttacctgt ctgacggtca
    20761 ccgatgatca attcacgctg accacgaccg attgggatca tggagtcaac ggctttataa
    20821 ccggtctgta ccggctgatc tacggactga cgttcgataa cgcccggagc gattgcttct
    20881 acagcagaga agccgtcgtg atccagcgga cctttaccgt cgattggtgc acccagagtg
    20941 ttaaccacac ggcccagcag gccacggcca accggaactt ccaggatacg gccagtacac
    21001 ttaactttca tgccttcggc aaggtcagcg tacggaccca taacaaccgc acctacagag
    21061 tcgcgctcaa ggttcagtgc gatagcgtaa cggttacccg gcagggagat catttcaccc
    21121 tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt cacttacaga aacaatagta
    21181 ccttcgttgt gagcttcact cacaacattg aactgagcaa tgcgctgctt gatcagttcg
    21241 ctgatttcgg tggaattcag ttgcatgctc cagtcccctt aagactgcaa gacgtctgca
    21301 aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca tatcacccgc tcggatgata
    21361 acgcctgcca ttacagactt atcgattttg caattcagct taactttgcg tgacagacgt
    21421 ttttccatcg cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc agcggaaatg
    21481 acgtctacct cagcggtagc ctcactcacg gcacgaaggt gaataaactg ctccagaaca
    21541 tccgggagcg cgttaagacg accattttca gccattaccc gaatcaggtt ctgaccgttt
    21601 tcgtccagtt gctcaccaca aactgcgata aacgactcgg cgagcgtttc tggcgcaagc
    21661 gcgccagaga gaagctctgc catttgttcg tttttggtta cctcggcggc aaacgccagc
    21721 atgtcctgcc agcgttctac actttggtgt tcgacggcaa agtcaaaagc tgctttggcg
    21781 taggggcgag ctaccgtaat aaattcagac atcagcccct ccctccttac agttcagcga
    21841 caagtttatc cacgatgtcg ctgttagcag cttcatccac ggaacgttcg atgatcttct
    21901 cggcgccagc aacagccagg atagcaactt gcttacgcag ctcttcacgg gcacgtttac
    21961 gctcggcttc aatttctgcc tgcgcctggg ccacgatttt agtacgttcc tgttctgcct
    22021 cagctttcgc ttcgtccaga atctgcgagc ggcgtttgtt cgcctgctcg atgattacct
    22081 gggcttccgc tttcgctttt ttcagctggt cggtcgcgct ggcctttgca aggtcaaggt
    22141 ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga cgtttttcga
    22201 tggctgccat taatggcggc catacgtact tcatgcagaa cagaacgaac aggacaaacg
    22261 cgatggcctg gccgaggatt gttgcgttaa gattcacagc acaatgcctc tatttagtta
    22321 acgttctgat attgctcttt aaataaaagc aacgcttact acgcgacagc gaacatcacg
    22381 tacagaccca gacctacagc gatcatcggg atagcatcca ccagacccat aacgataaag
    22441 aactgagtac gcagcagagg aatcagatca ggttgacgcg ctgcgccttc caggaattta
    22501 cccccgagga tgccgatacc gatcgcagca ccgattgccg ccagacccat catcacagcg
    22561 gcagccatgt acagcagatc catattcagg ttttccatga cagtctccag tttgtttcag
    22621 ttaaaacgta gtagtgttgg taaattaatg ttcttcagac gccatcgaca gatagacgat
    22681 cgtcagaacc atgaagatga aggcttgcag cgtaatgatc aggatgtgga aaatggccca
    22741 cggcacattc aggatccact gtgaccacca cggcaacaga ccagcaatca gaatgaaaat
    22801 cagctcaccg gcatacatgt taccgaacag tcgcaaaccg agtgaaactg gtttggacag
    22861 caggcttacc ccttcaagga ttaagttgac aggaatgaac gcccagtgat tgaacggctg
    22921 cagcgtcaac tctttcgtga agccgccgat gcctttcatt ttgatgctgt agaacagaat
    22981 caggataaat acgcccagtg ccatagacag cgttacgttc acgtccgcag acggaaccac
    23041 acgcagtgca ggcagaccca gtacatgttc agcaatgtac ggcagcaggt cgataggcag
    23101 taaatccatc aggttcatca ggaataccca gacgaagatc gtcagggcca gcggagcaat
    23161 cagcttgctt ttgccatggt acatgtcttt cacgctacca ttaacaaagc cgatcaccag
    23221 ctcaatcgcg gtctgaaact tacctggcac accgctggtc gcctttttgg ctacgctacg
    23281 gaataaaacc aggaacaaca gacccagcac caccgagaag aacatggagt caatattgat
    23341 tgtccagaag gtggctgggg ggttttgtgg atccaccagc gagaatgtac gcaggtccag
    23401 ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc gtcatatttt ctgaagccat
    23461 gatgcctttt accctttgtt gttaattaca gccggtgcca gtatctgaac caccagcacc
    23521 aaaacccacg taacgatcag cggcaagaat accgccttta aaaccgccaa cgccaccacc
    23581 agtaacacca acatcgccag aactttgaaa gcttcgccaa atgcgaatgt ccaggccacc
    23641 cggcctttcg ctggtgtata cgcctggtga cgccaggcaa atatcataaa caaaacgtta
    23701 ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc cccagaaggg gtctttgagg
    23761 ctgaacagca atccacttgc tatcaccacc agtaactgaa cgagcagaag cttccgagca
    23821 acgtttcgac tcacgagcga cacagacatc acgtttttca ctcctgctcc cttcgaggta
    23881 tgccgcgtgt cgtataaaac tttctttaag gcttagagtc aagcatcaaa aagcggtcaa
    23941 attatacggt gcgcccccgt gatttcaaac aataagtagc caaaaggtga ataaatgttt
    24001 aaatattttt ccagtgcata caattgcgac ttttccgcta accctgttcg atcatgaaaa
    24061 actgtaaata acgcgtaaac actagtgata aagcgtgctt cagatcacat attgcgcatg
    24121 ttcgcgcaca gtatatttat ttacttggca aatgatgcct ttgcaagttt atgatatttc
    24181 agtctaaaaa cagatactgt tttaataaat gacatttaca caacaaaaac cacccattga
    24241 catttttaat aatgttttaa cagccaatga tggttcttag cgccgatttt tagcagactg
    24301 atattttcac taatgactta ttttctgctt accaaaaaaa gccacgttat cttgttgata
    24361 caaaagagtg aacgtggcgt taaatgtaac cagttatatc agtagaaaac ttggttgttg
    24421 ttaacagtct aaccggtcaa ttttttatga tttttttgat aaaaattaaa ttttatttgc
    24481 tttaatcacc accagatgac gttcgccatc cagagctgga acctgaagtt taaccactga
    24541 ttcgacctga tattcttcgg gcaacaaagc gatttcatct tccggcattt gccctttcag
    24601 cgcgtagaaa cggccttgct caccaggaag atggtggcac cagctcacca tatcgttcag
    24661 agaggcaaaa gcgcggctaa ttacgccatc aaatggcggc tctgaaggaa actcttctac
    24721 cctgctctgt actggctcaa tattctccag tttaagctca tgttgcacct gacgaaggaa
    24781 acgcacgcgt ttaccaaggc tatccaacag agtgaaatgg gcttcaggac gcacgataga
    24841 gagtggaatg cctggcagtc ctggtccggt gccgacatcg ataaaccgtt caccttgcag
    24901 atacggtgcc accacaatgc tatcgagaat atggcgtacc agcatctcat taggatcgcg
    24961 gaccgaagtc aggttgtacg ctttgttcca tttatgcagc atattcacgt aggcaataag
    25021 ctggtttttc tggtgatcgg taagcgaaat acctgcgtct ttcagcagta aggagagttt
    25081 gttgagcacg gtgattacct gttcttgatg cgttgcctgg taagcgggtg cttaccaggc
    25141 atttttaatg cgttatgcgc tacgacgcag cataccctgt tttttcagcc acaccagcag
    25201 aatggagata gccgcaggcg tgacgccaga aatacgcgaa gcctggccga tagaggccgg
    25261 tttgtgatcg ttaagtttgg cgatcacttc gttagaaaga ccggatacct ggcggtaatc
    25321 cagtgtcgcg ggtagcaggg tgttctcgtt acgcagctgc ttttcgatct catcttgctg
    25381 gcgcgcgata taaccttcgt atttaacctg aatctcaacc tgttccgccg cctgttcgtc
    25441 tgtcaacgca ggggcaaacg gcgtcagcgt ggttaatttt tcataagtca tttccggacg
    25501 acgcagcaga tcttcaccac tggcttcacg ggaaagcggc gcagtcaggt gagcattcac
    25561 ttcggctgca gcttccgccg acggggttac ccaggtcgat ttcagacgct gacgctcacg
    25621 ctcgatattc tcaagtttct cgttaaagcg cgcccaacgt tcgtcatcca ccaggcccag
    25681 ttcacgaccg atttcagtca aacgcagatc cgcattatct tcgcgcagca tcagacgata
    25741 ttctgcgcgc gaagtaaaca tacgatacgg ttctttggtt cctaaagtgc acaggtcatc
    25801 aaccagtacg ccgagatacg cctgagaacg cgccggagcc caaccttctt tgtcagcaga
    25861 cagacgggca gcgttaagac cgaccagcaa accttgcgca gcggcttctt cgtaaccggt
    25921 agtgccgtta atctgaccag caaagaacag cccctggata aacttgctct ccagcgtcgg
    25981 tttcaggtcg cgtggatcga agaagtcata ctcaatggca taacccggac gcacgatctt
    26041 cgcgttttcc atcccctgca tggagcggac gatttgcatc tgcacatcga acggcaggct
    26101 ggtggagata ccgttcggat aaatttcgtt agaggtcagc ccttccggtt caaggaagat
    26161 ctgatgctga tttctgtcgg cgaagcgcat gactttgtct tcgatcgatg ggcagtagcg
    26221 tgggccgaca ccttcgatca cccctgcgta cattgggcta cgatcgaggt tactgcggat
    26281 cacatcatgg gttttctcgt tggtatgagt gatataacac ggcacctgct ggggatgctg
    26341 ggacgcattg cccataaacg agaataccgg cattgggtta tcgccatgct gttgcgccag
    26401 cacactaaag tcgatagttc gagcatcaat acgcggtggt gtcccggttt tcagacgacc
    26461 aacgcgcagc ggcagttcac gcaaacggcg agaaagcgga atggacggcg gatcaccagc
    26521 acgaccaccg ctgtaattat ccagaccgat atgaatttta ccgtcgagga acgtcccaac
    26581 ggtgagcacg acggctttgg cacggaactt cagtcccatt tgggtaacag caccgaccac
    26641 gcgatcgttt tcgacaataa gatcttcaac cgcctgctgg aagatcatca ggttcggttg
    26701 gttctccagc gccgtacgta ccgcctgacg gtagagcaca cgatccgcct gagctcgggt
    26761 agcgcgaacc gccggtcctt tgcttgcgtt tagtatccta aactggatac ccgcttgatc
    26821 gatcgctttc gccatcagac cgccgagtgc atccacttct tttaccagat gtcccttccc
    26881 aataccgccg atcgccgggt tgcagctcat ctgccccaga gtgtcgatat tgtgtgtcaa
    26941 aagcagagtc tgttgaccca tacgcgccgc ggccatcgcg gcctcggtgc ctgcatgacc
    27001 cccgccaatg atgatgacgt caaaaggatc cggataaaac atggtgattg cctcgcataa
    27061 cgcggtatga aaatggattg aagcccgggc cgtggattct actcaacttt gtcggcttga
    27121 gaaagacctg ggatcctggg tattaaaaag aagatctatt tatttagaga tctgttctat
    27181 tgtgatctct tattaggatc gcactaccct gtggataaca aggatccggc ttttaagatc
    27241 aacaacctgg aaaggatcat taactgtgaa tgatcggtga tcctggaccg tataagctgg
    27301 gatcagaatg aggggttata cacaactcaa aaactgaaca accgttgttc cttggataac
    27361 taccggttga tccaagcttc ctgacagagt tatccacagt agatcgcacg atctgtatac
    27421 ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc
    27481 gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg aatttttgag
    27541 ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac taccaatacc
    27601 gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa
    27661 aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa tcagccagat
    27721 ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc
    27781 ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc
    27841 gctgataaga gtgatatctg ccataaaccg ccacctttat taagagtggc gtattgtacg
    27901 ctgtgaacgc gttgggatct acctgtggaa aagtatggga ttaaaaaagc cgattatggc
    27961 ttgatggtac gcatgatcgg gttttgcagg acgatcaatg tctcggtgga ctgaatttca
    28021 tcaattgttt ggatcttgtt gataagtaca tgctggagag cgtcgatcga acggcacatt
    28081 acttttataa agatgctgta gtggcctgtt gtgtaatagg cttcagtgac ttcagcaagg
    28141 ctttccagct ttgccagcgc ggaagggtag tctttggcgc tctttaatat aatgccgata
    28201 aagcagccta cgtcataacc gagctgcttc gggctgacat caatacgcgc cccggtaatg
    28261 atccccgcct gcttcatttt ctctactcga acgtgaatcg tccccggact gacgccaaat
    28321 tgtttcgcca gttcggcgta agcggtgcgc gcattgccca ttaatgcttc caggatgcca
    28381 cggtccagat tgtcgatcag ataattttcc ataggatttt cttatgcaga ttgatgattc
    28441 attctatttt agccttcttt tttaatgaat caaaagtgtg ttaggctttt tattgaatga
    28501 ttattgcatg tttgtcggtt tttgttgctt aatcataagc aacaggacgc aggagtataa
    28561 aaaatgaaaa ccgcttacat tgccaaacaa cgccaaatta gcttcgtgaa atctcacttt
    28621 tctcgccaac tggaagaacg tctggggctg atcgaagtcc aggcgccgat tcttagccgt
    28681 gtgggggatg gcacgcagga taacttgtcg ggctgtgaaa aagcggtgca ggtaaaagtg
    28741 aaagctctgc ctgatgccca gttcgaagtg gttcattcac tggcgaagtg gaaacgtcag
    28801 accttagggc aacacgactt cagcgcgggc gaagggctgt acacgcacat gaaagccctt
    28861 cgccccgatg aagaccgtct ttctccgttg cactcggtct atgttgacca gtgggactgg
    28921 gaacgcgtaa tgggcgacgg tgagcgtcaa ttctcgactc tgaaaagcac ggtagaggcg
    28981 atctgggcgg gaattaaagc aaccgaagct gcggttagcg aagagtttgg cctggcaccg
    29041 ttcctgccgg atcagatcca cttcgtacac agccaggagt tactgtctcg ttatccggat
    29101 ctcgatgcca aagggcgtga gcgggcgata gcgaaagatc ttggcgcggt attccttgtc
    29161 gggattggcg gcaagctgag cgatggtcat cgccacgacg tgcgcgcacc ggattatgat
    29221 gactggagca ccccgtcaga gctgggtcat gcgggtctga acggcgatat tctggtgtgg
    29281 aaccctgtac tggaagatgc gtttgagctt tcttccatgg ggatccgcgt ggatgccgac
    29341 acgttgaagc atcagttggc gctgaccggt gatgaagatc gtctgcagct ggaatggcat
    29401 caggcgctgc tgcgcggtga aatgccgcag accatcggcg gcggtatcgg ccagtctcgt
    29461 ttgaccatgc tgctgctgca actgccgcat atcggccagg ttcagtgtgg tgtatggcca
    29521 gctgctgttc gcgagaacgt cccttctctg ctgtaataat ttatcgccgc cagcgtctga
    29581 gcaggcggct tcgcatcccg gtatcaaagc gccagatatg atcgaaaatg cgcatgatgc
    29641 cgggtttgcc gtgtgccgac atcgccacgg catgaaagcg atgctgatgt acccgctgca
    29701 gctctttcac tttactcgtc acgtcgtcag gcaaccgctg agcgataaaa tcagaaatca
    29761 ccaccgcatc ggcatcaaac cattccctgc tttgcaagcg ttccataatg gcgcgaaaac
    29821 aactggcaag atcggtgccg ccacgaaact gctggcttaa aaaacggatt gcttgttcga
    29881 tgccttgggg gcctgaaagc tcataacgga cgatctcggt ggaaaatagc ataatatagc
    29941 agcgccggtt ttcagcgaga gcaatgcgca tcaaggccag gcagaacgct ttcgcacact
    30001 gttcattaaa gccgcccatt gagccggaag tatccacaca gacaataaac ggcccgcgcg
    30061 gctgttcatc gtaatcttta tgtaccaccg gacgttcgat cactttttca cgccacgact
    30121 caccgtgcag gcgataggtg agcaactgtt tttccaccag ccgacggtaa aactcatact
    30181 ccagttccgt tatccctagt gtcgccagtt ctggcggcag aagacgtaaa atatcatcgc
    30241 tttgttgcag accatcaacc tgctcaggaa ccgtcgccgg ttcgcgcacc atggtgcgga
    30301 aggtttccat ctgcgcatcg ttgcgcggta ttgatttggc ttcccgagaa cgccccagct
    30361 gctctgccag gcgtttcagt tccggctgtt cgttaagaaa ttcaccgtat ttcacaatca
    30421 actgatagtc gccacgttta agctgaccgg cgctcatatc ccacagacga ccagctgcag
    30481 tattgttatc tgcgagaatc ggttcaagtt gtccgctcag cgtcatgcgt tcttgaactt
    30541 cactcaacag ttgttcgcgt tcttcttcta atagctgttg atttaacgtc gttgcttgca
    30601 cgatcagact taaacgccaa cgctggagaa aaagcgtgtg taacgctgaa gttattgtgc
    30661 tgttagcatc aaccaactgc cgggcttgtt ctgcccatgg ggaattcaga cgatgcagta
    30721 agtccaggat ctgtggtagc tgcacaataa actgcggcgt ggagaggagc tggctttgct
    30781 gatagcacat cacctcttcg gtgagttccg gcgggactcg ggcatctttc agccgactgc
    30841 gcagcgcctc acgccagcgg ggaacatcat cagtgattgc cgccttcagg cgtgggaatt
    30901 tttcaaagaa gaccgccagc tgcggtgagg ccagcagtgc gatgatcatc tcttcgatca
    30961 atccctcttc gctgacggcc agcatcacat taagcgtatc cagcgttagc attgttgtgc
    31021 ctggtgaatc tgtgcaccga catcctgtag gctggcttca atgcgaccta accagtcgcc
    31081 agggataaac agacattttt gctgttcgct gaataaagcg tgttgcttgc gccagtcgct
    31141 ttcaagctct tccagttgtt gttttatttc accaggcaga ccttcagccg atgaaccggg
    31201 aagtgccagc gtactgcctt gtaaacttac atcgcgaacg acaagatgtt gggcgctatc
    31261 aacttccaga ttcagtttct gggcaaagcc gataccgttc agtttaccgc gaatttcacc
    31321 acctttgctc agccactgtt ccagcgcgct acgctcaaag gagatatgaa ccacttccat
    31381 atcatgcagt tttaacggtt tttgcagcag tagagtcaga gtggaagcag taacgttaac
    31441 aggaagttga tactgctgac gacggctgaa aatgccgccc agacgaatta ccgttaaggc
    31501 tgttttatcg ctttgttgct gctggagttg caggtgacgt tgcacaatcg cgccaaggcg
    31561 agtcaacatc ccttgctgtt gccaggcatg accggtcatc aatacatcaa tttgttgttg
    31621 tatcaaattc aggctttgcg cgtcatacca caggcaatct ttcagcaaaa tgagatctac
    31681 cggggcaaca gcactgcgac cgctaaaaaa ggcgctggcc tgcaataatc ggatcgcttt
    31741 tttccagcga cgatccgaga cataaggcgc atccggtaat ttatccagtt gcaggcgcag
    31801 cataaaaatc agctcaaata catgatcggg cagcgtaatt tcaccaatct ctttctgcca
    31861 gcgttcatat tcttcatctg tgacctgcaa ggcatcagga accggattgt cgttttcatc
    31921 ctgttgactg gtcagcatgg agcggaaatt cgctttatcc tgcactttat ctaaccacag
    31981 acgaatcagc atgcggtcat ataacgcttc cagactgctg tctgcttccg gcagctcgtt
    32041 ggaggccgcc accagcagac gcatcgggat tttttctacg tgtgcaccgt tgcggaactg
    32101 gcgctcgtta atggcggtga gcaaggtatt aagaattgcc gggcccgctt tccagatctc
    32161 atccagaaag acaatttctg cttccggcag gtaaccgctg gttaaacgtt catagcgccc
    32221 ttcatctttt agcgcctgaa tagaaagggg accaaaaact tcttccggcg tggagaagcg
    32281 ggtcatcaga tattcaaacg cgcgggcatt ctgaaaggcg aattttaagc gccgggcgat
    32341 caaacttttg gcaatacctg gcgggccaag gaggaacaca ctttcaccac ttaatgccgc
    32401 taataaacac aagcggatgg cgtggctacg ttcataaagc cccttttcca acgaactgct
    32461 caggcgggaa attctttccg ctaataaatg agggtgagcc ataatgaagt ggcgtccttt
    32521 cgtcaaaagt tctgcgtaaa ttgcgagtat agacgtttct tgctggtggc taaaatagtc
    32581 tcaaagg
//
LOCUS       DAZPJR010000031        32576 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPJR010000031 DAZPJR010000000
VERSION     DAZPJR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.032"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            209..1582
                     /gene="aroP"
                     /locus_tag="ACHVB7_001345"
     CDS             209..1582
                     /gene="aroP"
                     /locus_tag="ACHVB7_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR1913374.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1625..2479)
                     /gene="ampE"
                     /locus_tag="ACHVB7_001346"
     CDS             complement(1625..2479)
                     /gene="ampE"
                     /locus_tag="ACHVB7_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR1913375.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2476..3027)
                     /gene="ampD"
                     /locus_tag="ACHVB7_001347"
     CDS             complement(2476..3027)
                     /gene="ampD"
                     /locus_tag="ACHVB7_001347"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR1913376.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3115..4008
                     /gene="nadC"
                     /locus_tag="ACHVB7_001348"
     CDS             3115..4008
                     /gene="nadC"
                     /locus_tag="ACHVB7_001348"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR1913377.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4211..4651
                     /gene="ppdD"
                     /locus_tag="ACHVB7_001349"
     CDS             4211..4651
                     /gene="ppdD"
                     /locus_tag="ACHVB7_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR1913378.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4661..6047
                     /gene="gspE"
                     /locus_tag="ACHVB7_001350"
                     /pseudo
     CDS             4661..6047
                     /gene="gspE"
                     /locus_tag="ACHVB7_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            6037..7239
                     /gene="hofC"
                     /locus_tag="ACHVB7_001351"
                     /pseudo
     CDS             6037..7239
                     /gene="hofC"
                     /locus_tag="ACHVB7_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(7274..8317)
                     /gene="guaC"
                     /locus_tag="ACHVB7_001352"
     CDS             complement(7274..8317)
                     /gene="guaC"
                     /locus_tag="ACHVB7_001352"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR1913379.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8473..8517)
                     /locus_tag="ACHVB7_001353"
     CDS             complement(8473..8517)
                     /locus_tag="ACHVB7_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913380.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8542..9162
                     /gene="coaE"
                     /locus_tag="ACHVB7_001354"
     CDS             8542..9162
                     /gene="coaE"
                     /locus_tag="ACHVB7_001354"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR1913381.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9162..9905
                     /gene="zapD"
                     /locus_tag="ACHVB7_001355"
     CDS             9162..9905
                     /gene="zapD"
                     /locus_tag="ACHVB7_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR1913382.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9915..10112
                     /gene="yacG"
                     /locus_tag="ACHVB7_001356"
     CDS             9915..10112
                     /gene="yacG"
                     /locus_tag="ACHVB7_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR1913383.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10212..10601)
                     /gene="mutT"
                     /locus_tag="ACHVB7_001357"
     CDS             complement(10212..10601)
                     /gene="mutT"
                     /locus_tag="ACHVB7_001357"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR1913384.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10661..13366)
                     /gene="secA"
                     /locus_tag="ACHVB7_001358"
     CDS             complement(10661..13366)
                     /gene="secA"
                     /locus_tag="ACHVB7_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR1913385.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13428..13940)
                     /gene="secM"
                     /locus_tag="ACHVB7_001359"
     CDS             complement(13428..13940)
                     /gene="secM"
                     /locus_tag="ACHVB7_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR1913386.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14171..15088)
                     /gene="lpxC"
                     /locus_tag="ACHVB7_001360"
     CDS             complement(14171..15088)
                     /gene="lpxC"
                     /locus_tag="ACHVB7_001360"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR1913387.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15189..16340)
                     /gene="ftsZ"
                     /locus_tag="ACHVB7_001361"
     CDS             complement(15189..16340)
                     /gene="ftsZ"
                     /locus_tag="ACHVB7_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1913388.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16401..17663)
                     /gene="ftsA"
                     /locus_tag="ACHVB7_001362"
     CDS             complement(16401..17663)
                     /gene="ftsA"
                     /locus_tag="ACHVB7_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR1913389.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17660..18490)
                     /gene="ftsQ"
                     /locus_tag="ACHVB7_001363"
     CDS             complement(17660..18490)
                     /gene="ftsQ"
                     /locus_tag="ACHVB7_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR1913390.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18492..19412)
                     /gene="ddlB"
                     /locus_tag="ACHVB7_001364"
     CDS             complement(18492..19412)
                     /gene="ddlB"
                     /locus_tag="ACHVB7_001364"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1913391.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19405..20880)
                     /gene="murC"
                     /locus_tag="ACHVB7_001365"
     CDS             complement(19405..20880)
                     /gene="murC"
                     /locus_tag="ACHVB7_001365"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR1913392.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20934..22001)
                     /gene="murG"
                     /locus_tag="ACHVB7_001366"
     CDS             complement(20934..22001)
                     /gene="murG"
                     /locus_tag="ACHVB7_001366"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR1913393.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21998..23242)
                     /gene="ftsW"
                     /locus_tag="ACHVB7_001367"
     CDS             complement(21998..23242)
                     /gene="ftsW"
                     /locus_tag="ACHVB7_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR1913394.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23242..24558)
                     /gene="murD"
                     /locus_tag="ACHVB7_001368"
     CDS             complement(23242..24558)
                     /gene="murD"
                     /locus_tag="ACHVB7_001368"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR1913395.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24561..25643)
                     /gene="mraY"
                     /locus_tag="ACHVB7_001369"
     CDS             complement(24561..25643)
                     /gene="mraY"
                     /locus_tag="ACHVB7_001369"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR1913396.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25637..26995)
                     /gene="murF"
                     /locus_tag="ACHVB7_001370"
     CDS             complement(25637..26995)
                     /gene="murF"
                     /locus_tag="ACHVB7_001370"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR1913397.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26992..28485)
                     /gene="murE"
                     /locus_tag="ACHVB7_001371"
     CDS             complement(26992..28485)
                     /gene="murE"
                     /locus_tag="ACHVB7_001371"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR1913398.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28472..30238)
                     /gene="ftsI"
                     /locus_tag="ACHVB7_001372"
     CDS             complement(28472..30238)
                     /gene="ftsI"
                     /locus_tag="ACHVB7_001372"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR1913399.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30254..30619)
                     /gene="ftsL"
                     /locus_tag="ACHVB7_001373"
     CDS             complement(30254..30619)
                     /gene="ftsL"
                     /locus_tag="ACHVB7_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR1913400.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30616..31557)
                     /gene="rsmH"
                     /locus_tag="ACHVB7_001374"
     CDS             complement(30616..31557)
                     /gene="rsmH"
                     /locus_tag="ACHVB7_001374"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR1913401.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31559..32017)
                     /gene="mraZ"
                     /locus_tag="ACHVB7_001375"
     CDS             complement(31559..32017)
                     /gene="mraZ"
                     /locus_tag="ACHVB7_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR1913402.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7736 a         9155 c         8033 g         7652 t
ORIGIN      
        1 caaacggttt ctttttaagc aactcatctt caaccatgca taaagcgggt gcattcgctg
       61 ccgcatacca ttattcttga tctgacggaa gtctttttgt aacaattcaa acttctttga
      121 tgtaaaaaaa ttaatacaac aaacggaatt gcaaacttac acacgcatca ctgcgtagat
      181 caaaaaaaca atcaccgcac gaggtttcat gatggaaggt caacagcacg gcgagcagct
      241 aaagcgcggc cttaaaaacc gccatattca gcttatcgcg ctgggtggcg cgatagggac
      301 agggctattc ctgggtagcg cctccgtaat acagtccgca gggccaggga ttatcctggg
      361 ttacgccatt gctggtttta tcgcctttct gatcatgcgt cagctgggtg aaatggtggt
      421 cgaagaacct gtcgcaggct cctttagcca ctttgcttat aaatactggg gcagttttgc
      481 cggtttcgcc tctggctgga actactgggt actgtacgtt ttagttgcca tggcagagct
      541 gactgccgtg ggtaaataca ttcagttctg gtatccggaa atcccaacct gggtttctgc
      601 cgccgtattc tttgtggtga ttaacgccat caacctgacc aacgttaaag tgtttggcga
      661 gatggagttc tggtttgcca ttatcaaagt tattgcgata gtagcgatga tcatcttcgg
      721 cggctggctg ctgttcagtg gcaacggcgg cccgcaggcg agcgttagca acctgtggga
      781 tcaaggtggt ttcctgccgc acggcttcac cggactggtg atgatgatgg cgattatcat
      841 gttctcgttc ggtggtctgg aactggtggg gatcaccgca gcagaagctg ataacccgga
      901 gcaaagtatc ccgaaagcga ctaaccaggt tatctaccgc atcctgattt tctatattgg
      961 ttcgttagcc gttctgctct cactgatgcc gtggacccgc gttaccgccg ataccagtcc
     1021 gtttgtgctg atcttccacg agttaggcga tacctttgtg gcgaatgcgc tgaacatcgt
     1081 ggtactgact gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca gccgtatgct
     1141 gtttggtctg gcacaacagg gtaatgcgcc aaaagcgctg gcgtctgtcg ataaacgcgg
     1201 cgtaccggtt aacaccattc tggtgtctgc gctggttaca gcattgtgcg tattgattaa
     1261 ctatcttgct ccggaatccg catttggcct gttaatggca ctggtggtat ccgcactggt
     1321 gatcaactgg gcgatgatca gtctggcgca tatgaagttc cgtcgcgcca agcaggaaca
     1381 aggcgtggta actcgcttcc ctgctctgct ttatccgctg ggtaactgga tctgcctgct
     1441 gtttatggcg gcggtactgg tgattatgct gatgacccca ggaatggcga tttcggtata
     1501 cctgatcccg gtatggctga tcgtgttagg tatcggctat ctgtttaaag agaaaaccgc
     1561 caaagccgta aaagcgcatt aatctctcta cgccctcacc catgcagggt gagggcaata
     1621 atctttacac caacgctccg taaatcgtca gtagcgcaat caccaccacg accacgaacg
     1681 aggttttctt cgccattgaa accgctgcct tcggcgtctc caccttatcg acatgcggtt
     1741 cacgcgccag agagaactgc gccagacgcg ttaacacctg atactgcgaa gtatggaagt
     1801 cacccagcga agcaaaccag gccggtaacg ctttctcccc atgaccgatc aaggcatata
     1861 ccacacccgc aagacgaacc ggcacccaat ccagtacatg aagcacggca tcaatgccgg
     1921 actgtaaacg atgatgcggc gtctggtatc gcgccagcca gtattgccat gcacgcaaaa
     1981 acgcataccc catcagcgta acgggtcccc aggttccccc cacaatcaac cagaacagcg
     2041 gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca cgcaaatact
     2101 cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt gagttcgcca gccatcgtgg
     2161 cacgggcatg gctatcatta cgtgaagcag ctgtcagata agcatgataa tgaagacgaa
     2221 ctttacctgc gccaatacac agcaaaccaa tcagcagcca caccagcagc gtgggaacgt
     2281 tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc gcaataatgg
     2341 tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac gcttcaagac
     2401 gatgatcaag ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt aacaccagta
     2461 aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc gcacgaaacc gtgcccaatc
     2521 aaatgcagga ccgggatcgg ttttccgatc cggcgcaata tcacaatggc ccgtcatgtt
     2581 attagcgata tcgggatagc gatctatcag tgcccgcgta accgccgcaa gctgttgata
     2641 ctgcgcatcg gtatacgcca gagtatcggt gccttcaagc tcaatcccaa tagaaaaatc
     2701 attgcagcgt tcacgcccct gatactgaga gactcccgca tgccaggcac gtttatcgaa
     2761 aggaacatac tggactattt caccatcacg gcgaatcaaa cagtgagcgg agacgcgcaa
     2821 atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca atagttccag tgaataatgc
     2881 gtcgatccac ggaccgccaa actcgcctgg cggcaggcta atattgtgca ccaccagcag
     2941 ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc ggcgcgcgcc
     3001 aaccagccac ccctgttcta acaacatgca ggatctcctt atatgtggtg ctaatacccg
     3061 gttcagagta gcatgtttct accttatgat tcgttagcta tctggagttt taacatgccg
     3121 cctcgccgct ataaccctga cacccgacgt gacgagctgc tggaacgcat taatctcgat
     3181 atccccggcg cggtggccca ggcgctgcgg gaagatttag gcggaacagt cgatgccaac
     3241 aatgacatta cggcaaaact tttaccggaa aactctcgct ctcatgccac ggtgagcacc
     3301 cgcgagaatg gcgtattttg cggcaaacgc tgggttgaag aggtgtttat tcaactggca
     3361 ggcgacgatg tcaccataat ctggcatgtg gatgacggcg atgtcatcaa cgccaatcaa
     3421 cccttgttcg aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg cactgcgctt
     3481 aattttgtgc aaaccctttc aggagttgcc agtaaggtac gccactatgt cgaattactg
     3541 gaaggcacca acacgcagtt gttggatacg cgcaaaacct tacctggcct gcgttcagct
     3601 ctgaaatacg cggtactttg cggcggcgga gcgaatcacc gtctggggct ttctgatgcc
     3661 ttcctgatca aagaaaatca tattattgcc tccggctcag tgcgccaggc cgtcgaaaaa
     3721 gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag tagagaatct ggaagaactt
     3781 gatgaagccc tgaaagcagg agccgatatc atcatgctgg ataacttcga aacagaacag
     3841 atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc tggcaacgtc
     3901 actgacaaaa cactgcgcga atttgccgaa acgggcgtgg actttatctc cgtcggtgcg
     3961 ctaactaaac acgtacaagc actcgacctt tcaatgcgtt ttcgctaatt gctgacagat
     4021 ttatccgctc catcgcgggc ggataatctc cccttcccca actttcttcg taacgcctcg
     4081 caaatttctt gtcttttgct gcaacgaaat aaaaaacctc ggaagctgct ttcccgtttc
     4141 gttttttgcc cctcgcttgt gctgatggcg tttgccaaag tagcgccaac caaatcaagg
     4201 agcgaaacag atggacaagc aacgcggttt tacacttatc gaactgatgg tggttattgg
     4261 catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc tgcgcaaagc
     4321 tgcactcacc gacatgctac aaacctttgt gccttaccgt acagccgtag agttgtgcgc
     4381 gctggaacat ggtggattag atacctgcga cggtggcagc aatggcattc cctcgcctac
     4441 caccacccgc tatgtttcag ccatgagtgt ggcaaaaggc gtggtgtcgc tgaccgggca
     4501 agaaagtctc aatggactaa gcgtcgtcat gacaccgggt tgggataacg caaacggcgt
     4561 caccggctgg gcgcgcaact gcaatattca aagtgacagc gcattgcagc aagcctgcga
     4621 agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc cacagctcac
     4681 cgccctgtgt ctgcgttatc agggagtctt gctggatgcc agcgaagagg tggttcatgt
     4741 tgcggtagtc gatgcacctt cgcatgagtt gctggacgca ttgcatttcg ctaccaccaa
     4801 acgtattgag atcacctgct ggacgcgcca acaaatggaa ggccacgcca gtcgcacaca
     4861 acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt tgctgactcg
     4921 aacgttacaa tctgcgctgg aacaacgtgc gtctgatatt catatcgaac cagcggacaa
     4981 tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg atgtttcacc
     5041 ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg atattgcgga
     5101 acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg ccgtctcatt
     5161 tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg gtattaaggt tgttacagca
     5221 ggtgaaccag gcactggatg ttaacacgct gggaatgcag ccgttacaac tggcggactt
     5281 tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta caggcagcgg
     5341 caaaacggtc acgctttata gtgccctgca aaagctgaat accgctgaca ttaatatttg
     5401 tagcgtcgaa gatccggttg agatccccat agccggacta aaccagacgc aaatccatcc
     5461 gcgtgccggg ctcacctttc agggcgtttt gcgtgcgtta ttgcgccagg atcctgacgt
     5521 catcatgatc ggagagatcc gctatggctt taacggcaga aattgccatt aaagccgcgc
     5581 aaaccggtca cctggtgttg tctaccctac acactaattc cacctgcgaa acgctggtac
     5641 gtttacagca aatgggagtc gcccgctgga tgctctcatc agcgcttacg ctggtaatag
     5701 cccagcgtct ggtacgtaaa ctttgcccac attgtcgcca gcagcaaggg gagcccatcc
     5761 atattccagt caatgtatgg ccgtcgccgc tgccccactg gcaggcaccc ggttgtgtac
     5821 attgctacca cggtttttat ggtcgtacgg ccttatttga agttctgccc ataacgccgg
     5881 tcattcgtca gcttatttcc gctaataccg acgttgaatc gctggaaacg cacgcacgac
     5941 aggcgggtat gcgtacgctt tttgaaaacg gctgcctggc cgtggagcaa ggcttaacca
     6001 cctttgaaga gttaatccgc gtactgggga tgccgcatgg cgagtaagca actctggcgc
     6061 tggcatggca taaccggcga cggcaatgcg caagatggga tgctatgagc agagagccgt
     6121 gctttgctgc tcatggcact acagcaacag atggttaccc cacttagcct gaagcgaatc
     6181 gccatcaatt ctgcgcagtg gcgaggagat aaaagcgcgg aagtcattca tcaactggcg
     6241 acgctactca aagccgggtt aacgctttct gaagggctgg cactgctggc ggaacagcat
     6301 cccagtaagc aatggcaagc gttgctgcaa tcgctggcgc acgatctcga acagggcatt
     6361 gctttttcca atgccttatt accctggtca gaggtatttc cgccactcta tcaggcgatg
     6421 atccgcacgg gtgaactgac cggtaagctg gatgaatgct gctttgaact ggcgcgtcag
     6481 caaaaagccc agcgtcagtt gaccgacaaa gtgaaatcag cgttacgtta tcccatcatc
     6541 attttagcga tggcaatcat ggtggttgtg gcaatgctgc attttgttct gccggagttt
     6601 gccgctatct ataagacctt caacacccca ctaccggcac taacgcaggg gatcatgacg
     6661 ctggcagact ttagtggcga atggagctgg ctgctggtgt tgttcggctt tctgctggcg
     6721 atagccaata agttgctgat gcgccgaccg acctggctta tagtgcggca gaaattgctg
     6781 ttacgcatcc cgattatggg ttcactgatg cggggacaaa aactcacgca gatctttacg
     6841 attctggcgc tgacacaaag tgcaggcatt acttttttgc agggcgtaga gagcgtcaga
     6901 gaaacaatgc gctgcccgta ctgggtgcaa cttctgacac aaatccagca cgatatcagt
     6961 aacggtcatc ccatctggct ggcgctaaaa aataccgggg agtttagccc gctctgtttg
     7021 caattagtga gaacaggaga ggcatccggc tcgctggacc tcatgttaga caacctcgcc
     7081 catcatcatc gggataacac aatggcgctg gcggataacc tcgcagcctt actggaaccg
     7141 acgttgctga tcataacggg aggaattatc ggtacgctgg tggtggcgat gtatctgcca
     7201 attttccatt taggcgatgc gatgagtggg atgggataac gctggcgtgt tgctccacac
     7261 cagcgttgga agattacagg ttgttgaaga tgcggttttc ttgttcctgc acacggataa
     7321 atgtggtgcg cttggtcagt tctttcaggc gtgaagcccc aacgtatgta caagctgaac
     7381 gcaggccgcc caaaatatca cgcgcggtat tttcaaccgg gcctcgcagc ggcagcttaa
     7441 cggttttacc ttctgctgcg cgatattccg caacgccgcc aacgtgacgt ttcatcgcag
     7501 actcggagct catgccgtag aacagcataa atttctcgcc gttctcctca acgatgcgac
     7561 cgccgctctc ttcgtggccc gccagcatgc cgccaagcat gacgaaatcg gcaccgccgc
     7621 caaaggcttt tgcgacatcg cccggcgtgg tgcagccacc gtcgctgacg atcattccgc
     7681 ccagaccgtg cgccgcatcg gcacattcga ttaccgcaga aagctgcgga taaccgacgc
     7741 ctgttttgac gcgagttgta caaacagaac ctgggccaat gccaacttta acgatatcag
     7801 caccggagag gataagctcc tcacacattt caccagtcac tacgttgcca gcacaaatgg
     7861 ttttggtcgg ccacgcttca cgcgctttcg caacgaactg cacgaagtgt tcggaataac
     7921 cattcgccac atcaatacaa acgaagttta atgccgggtt caggtcgaga atctgtttag
     7981 ttttttcgaa atccgcatca gacgtaccgg tagaaaccat cacatgtttc agcacatcag
     8041 cggaagaatt gttgataaac gcttgccact cttcgacaga atagtgttta tgcacagcag
     8101 tcaaaatatc aaaagaagcc agcgcagagg ccatagaaaa tgtacctacg gtgtccatat
     8161 ttgcggcgat aatcggcacg ccggaccagc tctgacctga atgtttgaag gtgaattgac
     8221 gttccagttc aacatcggaa cggcttttaa gagtggagcg tttagggcgg atgagaacgt
     8281 ctttaaaacc taacttcaga tcttcttcaa tacgcatgtg cggattcctg gggttaatgg
     8341 ctataagggt aagagcgcaa ctccagtgac gctatcatac gcactaatca atgcggcgca
     8401 agactgcgaa attgtctttt tttacgctac aatcccataa atttacctgg taaatagtga
     8461 gcgaacgttt catcaatttc gctttcgtgc actcattttt aacgttttga taatcatgat
     8521 gaattcccgg gaatataaat tatgaggtat atagttgcct taacgggagg cattggcagt
     8581 ggcaagagta ccgttgccaa tgcgtttgct gatctcggaa ttaacgtcat tgatgccgat
     8641 attattgcgc gtcaggtggt tgaaccaggt gcacctgcgc tacatgccat tgctgatcac
     8701 tttggcgcta acatgattgc tgctgatgga acattgcagc gccgggcctt gcgcgagcgg
     8761 atcttcgcca acccggaaga gaaaaactgg cttaacgccc tgctgcatcc gctgattcag
     8821 caagagacgc aacaccagat ccagcaagct acttccccct atgtactgtg ggttgtgcca
     8881 ttgctggtag aaaactcact gtataaaaaa gcgaatcgag tgctggtggt ggatgtcagc
     8941 ccagaaacgc aacttaagcg caccatgcag cgcgatgatg taactcgcga gcatgtcgaa
     9001 caaatccttg ctgctcaggc aacgcgcgaa gcccgccttg ccgtggcaga tgacgtcatt
     9061 gataataacg gcgcaccgga tgctatcgca tcggatgttg cccgcctgca cgcacactat
     9121 ttgcagcttg cgtcgcagtt tgtctcacag gaaaaaccgt aatgcagacc caggtccttt
     9181 ttgaacatcc actaaatgaa aaaatgcgta catggctgcg cattgagttt ttgattcagc
     9241 aactcaccgt taatttaccc atcgttgacc acgctggcgc gctgcatttc ttccgtaatg
     9301 tcagtgaatt actggatgtt ttcgagcgcg gcgaagtccg cactgagctg ttgaaagaac
     9361 ttgaccggca gcaacgtaaa ctccagacct ggattggcgt gcctggcgtg gaccagagcc
     9421 gtattgaagc attaattcag cagttaaaag cggcggggag cgtattaatt tccgcgccgc
     9481 gtatcgggca atttctgcgt gaagatcgtt tgattgctct ggtgcgtcag cgactgagca
     9541 tcccaggcgg ctgttgcagc tttgatttac ctacattgca catttggctg catctacccc
     9601 aggcgcagcg cgacagccag gtagaaacct ggattgccag cctgaacccg ctcacccagg
     9661 cacttaccat ggtgctggat ttaattcgcc agtcggcccc cttccgtaaa caaaccagtc
     9721 tgaatggttt ttatcaggat aacggtggcg atgccgactt gctgcgcctg aatctgtcgc
     9781 tcgattcaca gctttatccg caaatttccg gacataagag ccgttttgcc attcgtttta
     9841 tgccgctgga cactgaaaac ggacaggtac cggaacgtct ggatttcgaa ctggcctgtt
     9901 gctaaggagt aaaaatgtca gaaactatta cggtgaattg cccaacctgc gggaaaacgg
     9961 tggtgtgggg tgaaatcagc ccgtttcggc cattttgctc caaacgttgt cagctgatcg
    10021 acctcggaga atgggctgct gaagaaaaac gaatccccag cagtggcgat ctttccgaaa
    10081 gcgatgactg gagcgaagag ccaaagcagt gacatttgtc tgatgccgca cgtaggcctg
    10141 ataagacgcg gacagcgtcg catcaggcat cttgtgcaaa tgtcggatgc ggcgtgaacg
    10201 ccttatccga cctacagacg cttaagcttc gcaattaccg gttcattggc tggcggaaaa
    10261 tcatcggcat taagaccgac tagcgacatc cactcaccgg gttgcccttc tttaccccac
    10321 ggctcccctt cccagctttc gaccagccag aaccacagag ttatatgcct gtccgggaat
    10381 tcatattcca gtttttcaaa tagcgaaaaa tgttgtgggg taatcccgac ttcttcctga
    10441 agttcacgca ctaccgcctg ttccggcgtt tcgcccattt caattttacc gccgggaaac
    10501 tccagtttat tcgccatgtg cgcatctgct gcgcgacgcg ttataaagat ttcattgttc
    10561 tcgttgcgaa taatacctac cgcaatttgc agctttttca ttgtcttaaa cctataaaaa
    10621 aggcgcagaa tcctgcgcct tttacttcaa ctgttagctt ttattgcagg cgaccatggc
    10681 actgcttgta ttttttacca gaaccgcacg ggcaaggatc gttacgtcct actttgcgct
    10741 caccggtttg cgccgccagt gcagctgcgg ctgcagagtc gtcatcctga tggctaagct
    10801 gctgcatttg cgctaaacgc tcggcttcca tacgacgctg ttgttccagc tcctcaacct
    10861 cttcaggcat acgtacctgg actttgctca gcgtgctgat aacttcatat tttaacgact
    10921 ccagcatcgc tgcaaacatg gagaacgatt cacgtttgta ttcctgcttc ggatctttct
    10981 gtgcatagcc acgcaggtgg ataccctgac gcagatagtc catcgccgct aggtgctctt
    11041 tccacagaga gtcgagagtt tgcagcatga cgcctttttc gaagtgacgc atcatctcag
    11101 caccaaccac ttcttcttta cgctgataca cttcgatgga ctgcgccaga attcgctcac
    11161 gcagcgtctc ttcatgcagt tctggttctt tatccagcca ctcggcaatt ggcaaatcga
    11221 ggtcgaaatc gttcttcaga cgttcctgca gccccggaat atcccacatt tcttccagtg
    11281 actgtggcgg aatgtaggca tcaatggtcg ctttgaacac atcttcacga atgctgttga
    11341 tggtttcgct cacatcgctg acatccagca gttcgttacg ctgggagtaa atggcgcgac
    11401 gctgatcgtt agccacgtca tcatattcca gcagttgctt acgaatgtcg aagttacggc
    11461 tttcaacttt acgctgggcg ttggcaatcg ctttagtcac ccacgggtgt tcaatggctt
    11521 cgcctggctt catacccagt ttacgcatca tgccggatac tcggtcggaa gcaaaaatac
    11581 gcatcagcgc atcttccatc gacaggtaga aacgggaaga accagcatcc ccctgacgac
    11641 cagaacgacc gcgcaactgg ttatcgatac gacgggattc gtgacgctcg gtaccgatga
    11701 tatgcaggcc acctgcttcc agtaccgcat cgtgacgtac ctgccagtcg gctttaattt
    11761 tttcaatttg ctctgcggtc ggattttcca gcgcggcaac ttctgcctgc cagctaccac
    11821 cgagcacaat atctgtacca cgacccgcca tattggtcgc gatagtcacc gcagccggat
    11881 aacctgcctg agcaacaatc gccgcttcgt tggcgtggaa tttggcgttc aggacgttgt
    11941 gcttaatacc ggctttggtc agttcgtttg acaccagctc cgatttttcg atggagatag
    12001 tacccaccag caccggctgg cctttcgcag tacgttcttt gatatcttca atgatcgcct
    12061 gaattttttc cgcttcagtc atgtagacca ggtccggcag atctttacga atcattggac
    12121 ggttggtcgg aacaacgacg gtatccagct tgtagatgga gctaaattcg aaagcttcgg
    12181 tatcagcagt accggtcatc cccgccagtt tttcatacag acggaagtag ttctggaagg
    12241 tgatcgaagc cagcgtttgg ttttcgttct ggatctgcac accttctttc gcttccacag
    12301 cctggtgcag accatcggac cagcgacggc cctgcatggt acgaccggtg tgttcgtcaa
    12361 cgatgataac ttcaccatct ttaacgatgt agtcgacgtc gcgggtaaac agcgcatgag
    12421 cgcgcagcgc cgccgttacg tggtgcatca gcatgatgtt ggccggagag tacagagact
    12481 ccccttcatc catgatgccc tctttcacca gcagttcttc aatcagcacc agaccacgtt
    12541 cggtcaggtt cacctggcga gatttttcgt ccaccgagaa gtggccttcg ccctggaagg
    12601 tttcggagtc ttctttttcc tgacggatca ggtgcggaat aattttattc acgcgtttat
    12661 acatttccga gctgtcttct gccgggccgg aaatgatcag cggtgtacgc gcttcatcga
    12721 tcaggatgga gtccacttcg tccaccagcg catagtgcag tttacgctgt acacgttctt
    12781 cagggctgaa cgccatattg tcgcgcaggt agtcaaagcc gtattcgttg ttcgtaccgt
    12841 aagtgatgtc agctgcgtaa gcttcgcgct ttgccggtgc tggcatgccc ggcaggttga
    12901 taccgacagt caggccaagg aattcaaaca gcggacggtt gttttcggcg tcacgttgcg
    12961 ccaggtagtc gttgacggta actacgtgca cgcctttacc ggttagtgcg ttcaggtaag
    13021 caggcagcgt tgcggtcagg gttttacctt caccggtacg catttcggcg atgcagcgtt
    13081 cgttaagaac cataccgccg agtaactgaa cgtcgaagtg acgcatacca aagacgcgct
    13141 tacttgcctc acgtaccacg gcgaaagctt ccgggatcag attttccagc acttcgcctt
    13201 tttccagacg tgcacgaaac tctgcggttt tccctttcag ttcttcgtcg gagagttttt
    13261 ccatctccgg ttccatggca ttgatgatgt tgaccacttt gcgcatccgg cgcagggtgc
    13321 gatcgttacg actaccgaaa actttagtta acaatttgat tagcataata aaatctcaaa
    13381 cgccccgcgt tgcggagtta ataaaatgaa gtaaaggttt attgttgtta ggtgaggcgt
    13441 tgagggccag cacggatgcc ttgcgcctgg cttatccaga cgggcgtgct gaattttgct
    13501 tgtggggtaa aatgcgcata atcaatgcga taaccctttt cagacggcgt gccttcctgg
    13561 gtcagcagcg cgctgagcgt atccagtaat gcaagatgtt gcgcctgaag aggcaaagac
    13621 tcttcagcaa cgggcagtgt ttgcggtgcc attgcgaaag aaagatgacg gattaccgtg
    13681 cgaatggcat gttgatgcca gtaatcaacg gaatagttcg aattcgggcg gcgtgtgttc
    13741 gcttccagca aggccaattg accaaagtta actttggctg aaggctcgtg gttgcgggtt
    13801 gtcgcttttg cgggcgcgtt tggttcggcg gcgttgctga gcgcaggcaa acctaaactc
    13861 gccgcaacca tccctaataa gagatgcggc cagaagtagc gtttaccaaa ctgtcgccag
    13921 cgcgtcagta ttccactcac gttattgcca tcccgatcgt attcaaacgt atttagcgca
    13981 caaatcttat cattaaagcc tgcagtccac agcacgaatg aagataagtt gtgcggaaaa
    14041 gtgcttaaga aaacagcgtt cgcaccgttt tttatagctc ttataaaatt caaaaaaaaa
    14101 tgactggttc acctggccgg agagagtgcc agatttgcca gtcgaatttt atacgacagt
    14161 ataaatgtcg ttatgccagt acagctgaag gcgctttgaa ggccaacggc agttctgcgt
    14221 cgtcctggaa ggtcacatat tcccaggctt cctgtttcgc caggacagcc tgcagcagtt
    14281 tgttattcag tgcatgaccg gatttataag cggtaaatgc accaataata ttgtgaccac
    14341 acatgaacaa gtcaccgatc gcatcgagca ttttgtgacg cacaaattcg tcttcaaaac
    14401 gcaggccgtc ttcgttcagt acgcgataat cgtcaacaac gatggcacaa tcgaagctgc
    14461 cgcccaggca caaaccacgg gactgcagat attcgatatc acgcatgaaa ccgaacgtac
    14521 gcgcacggct gatctggcgc ataaacgcat cagcggagaa gttcatcgca tagcgctggt
    14581 tgctggaatc aatagccgga tggttaaaat cgatggtgaa atccagcgaa aaaccattgt
    14641 acggcttaaa ttcagcccac ttatcgccat cttcgacacg aacagtctct ttgatgcgaa
    14701 caaatttttt ggcgcagttc aactcgtcga taccggcgtc aagcagcagg tatacaaacg
    14761 gagcggcgct gccgtccatg atcgggattt ccggcgcgtt aacttcgata acaatgttat
    14821 cgatgcccag gcccgcgaga gcagcattga ggtgctctac ggttgaaatc cgtacatcat
    14881 gctcgttgac cagacacgta cagagcatgg tatcacgcac agatttggca tcggccggga
    14941 aatctaccgg tggattcaag tcggtgcgac gatagatgac cccggtgttg gccggcgcag
    15001 ggcgtaacgt cagagtgact ttcttgccgg tatgtaaacc gacacccgtc gcctgaacga
    15061 tacgtttaag tgtcctttgt ttgatcatcg tattatctcg ccaaattacc tatccaaccg
    15121 aagtgtacta tacattcggc gggccagttt agcacaaaga gcctcgaaac ccaaattcca
    15181 gtcaattctt aatcagcttg cttacgcagg aatgctggga tatccagata atccggctct
    15241 ttcgcagttt gcggcgcatt gtcattcacg actttagcaa ccggcttctg ctcctgggtc
    15301 agcggagcca tcccatgctg ctggtagcga tccatcactg gctgctgaac ctgcttattg
    15361 gtcaccagag tgatttcagg acgtttgtcc atgccgatac ctgtcgcaac aacggttacg
    15421 cgcagctcgt cattcatatc cgggtcaaga gaagtaccga taaccacagt cgcgttgtcg
    15481 gaagcaaatg cacggatggt gttacctacc gtttcgaact catccagacg caggtcgaag
    15541 cccgccgtga tgttaaccag cacgccgcgc gcgccagaca ggtcgatatc ttccagcagc
    15601 ggagaagaga tagccatttc agcagcttct tccgcacggt cttcaccgct cgccacgcca
    15661 gaacccatca ttgcgtagcc catctcagac attacggtgc gtacgtctgc aaagtccacg
    15721 ttcatcaaac ccggacgagt aatcagttca gcgatacctt gcacagcgcc tttcagtaca
    15781 tcgttcgctg cgccaaacgc atccagcagg gagataccgc ggcccagaac tttcagcagt
    15841 ttgtcgttcg ggatagtgat cagagagtcc acatgcttgg acagttcagt gatcccctgc
    15901 tccgcgaatg ccatacgctt cttgccttca aagttgaaag gcttagtgac gacagcaacg
    15961 gtcaggatac ccaaatcttt tgccacttca gcgacgactg gtgctgcacc tgtaccggta
    16021 ccaccaccca tacccgcagc aataaagacc atgtctgcac cttccagcgc cgcacgcaat
    16081 gcatcgcgat cctcatcagc cgcattgcga ccaacttctg gattagcgcc agcgcccagt
    16141 cctttggtga taccgctacc gatttgaatc gtctgtccaa ccgctgtttt acgcagcgct
    16201 tgtgcatcgg tatttaccgc gaagaattca acaccttcaa tacgctcgcg caccatgtgt
    16261 tcaacagcat taccgccgcc gccgccgacg ccgatgactt taatcaccgc gtcattggta
    16321 agttccattg gttcaaacat agtttctctc cgatttgtgc ctgtcgcctg aggccgtaat
    16381 catcgtcggc ctcataaaaa ttaaaactct tttcgcagcc aactattgag tcgcttgatc
    16441 cacgagccaa ctgatgctgt aacacgtttt tctacttcag cttcaccgtt aagatgtgac
    16501 tctttcccat agtgaagcaa tcccaccgcc gtcgaataat acggctcctg agcataatcc
    16561 gttaaaccgg taatgttcag cggcgcgccg atacgcactt gcgtatgaaa cacgcgctga
    16621 gcacaggctg caagaccttc gatctgcgct gcgccaccgg ttaatacaat gcctgccgcc
    16681 aggtgatgtt taaccccttg ttggcgaagc ttttcctgca actgcaatat ctcttcgttg
    16741 accaggttga gcagctcggt atagcgcggc tcgatcacct ctgccagggt ctgacgttgc
    16801 agactccgtg gcggacgacc gcctacgctc ggcacttcca cgctctcatc ttttccaacg
    16861 atggaaccca gcgcacaacc gtggcgaact ttaatcgctt cggcgtcgct tggcggcgtg
    16921 ccaaaggcgt aagcgatatc actggtcacg acattgccag cataaggaat taccttagtg
    16981 tggcgcaatg ccccaccggt ataaacggcg atatccattg taccaccacc gatatcgacg
    17041 acgcagacac ccagttcacg ttcatcttcc gtcaataccg aataacttga tgccagtccg
    17101 gcaaatatca gttggtcaac tttcagccca caacgttcaa ccgctttgac gatgtttttc
    17161 gccatatcgt tgtgacatgt gatcaggtgc acttttgcct gcatccgcac gcccgaaagt
    17221 cctaccggat tcttgatccc ttcctgatag tcaatcgcat actcttgcgg gatcacatgc
    17281 agcacacgat gttcatcgcg cacacgcacc gatttcgcgg tatggacgac gttttccaca
    17341 tcttcttgcg tcacttcttc ttcagaaata ggcaccatac caatttcatt ctggcagctg
    17401 atgtgcttac cagaaagcgc cagatatacc gaagagatct gacaatctgc catcaattct
    17461 gcctggtcaa tggcgcgttg tacgcacttg accacggatt cgaggtcgtt caccccgcct
    17521 ttatccatac cacgtgacgg gcagctgccc acgccaatga tattgaccat accgtcgggc
    17581 agaacttccc ctactaaagc ggcaaccttc gcggtaccaa tctccagtcc tactaccagt
    17641 tttctgtccg tcgccttgat cattgttgtt ctgcctgtgc ctgattttgt tgctgagtag
    17701 attcctctgg cggcaagggc gcccagccta ctgccgctcc agagtcataa cgcaaatcaa
    17761 cgtagctaat ccgtttgcca tcggtttgcg cctgctgctg taaaaccgga taaagttcta
    17821 caaagcgagc caaacgtttc atcgtatcgc cccggccaag attgagctta atatcgttat
    17881 tcagcgtcaa ctgccaggaa cgccgcgcgg tcatcgccgc ttccttcaga gtaaatctgt
    17941 cctttgccag catctgcccc atttcgcgat agccctgcaa cacttcattg gcgctgcctt
    18001 ccgggccata cagcattgga agcacctgct tgctggtgcg atctggcggc acgctgaagg
    18061 tgtttccttc cgcgtctacc atatgttgat cattccaccg cgcaatcggc acatattcaa
    18121 ccagatgaat cttcaattca tcaggccact gctttctgac gctcacctgc ttaatccacg
    18181 gcaggcgttg ttctatttgc gtctggatga tgttgacatc ctgggtcata aaggtacccg
    18241 gctcacccaa tgccaggatc gactgccgga tatcgtcatt acgcgtgtaa tggcgttcac
    18301 cggtcaacac cagctttgag agcggcaggc gttgcgcatc ttccatccag cccaacacga
    18361 cccagccgct caccaacact gtcgttaaaa cggtcagcag gaaaaggatc cccgccagac
    18421 gcgttccatt attgcggcga gaagaaacct cttcttcgct atttcgcgtg ttcagagcag
    18481 cctgcgacat attagtccgc cagttccaga attcgtacta ccaactgcga gaagctcata
    18541 cctgcctgac gtgccgccat cggcaccagg ctgtggctgg tcatacccgg tgaggtattg
    18601 gcttccagca gataaaactg tccatcgctg tccagcataa cgtcaatacg tccccatcct
    18661 ttgcaaccta acgtcgtcca tgctttcagc actaatgcct gcaaattggc ctcttgtgac
    18721 gcttccagac ctgcggggca gaaatactgt gtctcatcag agagatactt cgcctcataa
    18781 tcatagaagg ttccggacgg ttgaatacgt attgacggta aaatttcttc accgagtatc
    18841 gcaaccgtga actccggccc acttagccat ttttcaatca atacttcttc atcgtgctga
    18901 aatgccaatc ttaatgcatc ttgtagagca ttttctgcta ctacttttga cattcccaca
    18961 ctggaacctt cgcggctcgg cttaacgata accggcaaac ccagagcaga aatttctgct
    19021 aactgcttat cgctcaggcc tttttcaaac tctgcgcggg ttaacgctac ccacggcgcg
    19081 accggtaaac cggcaccttg ccacagcagt ttgctgcgta gtttatccat tgaaagcgca
    19141 gatgccatca ctccgctgcc ggtataaggc aagcccatca gctcaagcat gccctgcagc
    19201 gtaccatctt caccgccgcg accgtgtagc gcgataaaca ctttctgaaa acccatcgac
    19261 ttcagttgcg tcacgtcgac ttctttcggg tcgacaggat acgcatcaat accgccttca
    19321 cgcagcccgg ctaacaccgc tgcgccagaa ttcagagaaa cttcccgctc agcggaagtc
    19381 ccgcccaaca ggaccgcaat tttatcagtc atgttgttct tcctccggag tttgcggctt
    19441 cagtttgatt tcagctaaag aacgggcaat ttttccgata ttaccagccc cctgaacgag
    19501 aatcaggtcg ttaccgatta ataccggtgc cagcatctcg gctacctgcg cagggtccgg
    19561 caccagaatg ggatcaattt tcccacgtcc acgaattgtg cgacacagcg aacggctgtc
    19621 cgctcccgga attggcgctt cgccagccgg atacacttcc agcatcaaca gggtatcaac
    19681 ctgcgtcagc acattggcga aatcatcata caggtcgcgc gtacgggtaa aacggtgcgg
    19741 ctgaaacagc attactaggt ttttatccgg ccagcctgcg cgcgccgctt taatggtggc
    19801 gtcaacttcc gtcgggtggt ggccgtagtc atcgaccagc attgccgtac cgcttttacc
    19861 attcactggc tccagcggga attcaccgag gaaatcaaaa cggcgaccag tcccctggaa
    19921 gctctccagc gcacgcaaaa tagcctcgtc gtcaattccc tcttccgtag caaccgcaac
    19981 cgcagctgcg gcgttcagcg cgttatgacg gcctggcgca ttcagggtga cgcgcatcgg
    20041 ctctttgtcc tggcgcagca gcgtaaagtg cccctgcggg ccaatctgct gataatcttc
    20101 tacacgcacg tcggcatctt cgctgaagcc gtaagtcgtg gtctgacgtc ccacacgcgg
    20161 taacaattcg cggatcaccg gatcatcaac acacatcacc gcacgaccgt aaaacggcag
    20221 gttgtgcaga aaattaataa aagtctgttt taaattctca aagtcgccct ggtaggtatc
    20281 catgtggtcg gcttcgatat tggtgacaat cgccaccatc ggttgcagat gcaggaacga
    20341 tgcatcactc tcatctgctt cggcaatcag gtaccgacca tgccccaaac gcgcatgaac
    20401 ccccgccgct tttaccagcc cgccgttaac gaaggttggg tcgagccccg cttctgcgta
    20461 gatgctggaa accatcgcgg tggttgtcgt tttgccgtgc gttcctgcaa tggcgatgcc
    20521 atgacgaaaa cgcattaact cagccagcat ttcggcacga cggatcaccg gaatacgcgc
    20581 ttcatgagct gcgacaattt ccgggttatc ggcagaaatg gcgctggaaa caacgaccac
    20641 gctggcatca cgtacgtttt ccgggcgatg gttgaaataa atcgtcgctc ccagattcat
    20701 taactgctgc gtgaccggat ttggcgctaa atcggaacca ctgatctgat aaccttcatt
    20761 ggccagaact tcggcaatac cgcccatacc ggcaccaccg atgccgacaa aatgtatgtg
    20821 ccgaacgcga cgcatttcgg gcacgatgga acgcagtttt gccaattgtt gtgtattcat
    20881 tctttacgcc attaacttct taaattcata cgatgcaaaa ggcatcgcta caattacgcc
    20941 cgggcagccc ggctcacttc atttgccact cgctcggtgg catccggaat ggatgcagcg
    21001 cgggcgcgtt ctgccatggt taataaggtt tctcgcgacc acccggccag ggtgttggcg
    21061 acagcatcca cgctaagctg tggctgctcg ataattttgg ctgcgcccgc tttttccagc
    21121 ggtagcgcat tccagtattg ctggcggtct ttatgttgaa acggcacaaa caacgccggt
    21181 agtcctgccg cggcgatttc actcaccgtt aaagcaccgg agcggcaaac gacgacatcc
    21241 gcccacgcat acgccgccgc catatcatca ataaattccg tcactttatg ctgcggttgc
    21301 cccgcttcgg catacgcctg ttcaacggat tgttgcgaac ctttaccgct ctgatgccag
    21361 atagtgactg aatcacccag tttcgcagca acctgcggca ttgtctggtt aagaatgcgt
    21421 gcgccctggg aaccacccac taccagcaca cgaaccggac cttcacgtcc agccaaacgt
    21481 tgctgcggca acggcagcgc cagcacatcg gtacgcaccg ggttacccac cacttccgca
    21541 ttagggaaag cgcctggaaa cgcctgcatc actttggtgg caatcttcgc cagccattta
    21601 ttggttaagc ccgcaatacc gttttgttca tgaagtacaa ccggaatgcc taacgaccac
    21661 gcggccagac cacctggacc tgatacatag ccgcccatac cgagtaccac gtcaggtttg
    21721 tacgctttca taatcgcccg cgcctgacgc caggcgttga agatacgcag cggcgcagct
    21781 atcagtgctt ttataccttt tccacgcaga ccagagatac gaatgaaatc aatttcgatg
    21841 ccatgttttg gcactaagtc cgcttccata cggtcggcag tccccagcca gcgaacttgc
    21901 caaccctgag ccattagatg gtgcgcaacc gccagtcccg ggaatacatg tccaccggtt
    21961 ccgcctgcca tcaccattaa tcgctttcct tgaccactca tcgtgaacct cgtacaaacg
    22021 cctgcgcttt ctccagacgc gtttcataat caatacgcaa cagcatcatg atggctgtcg
    22081 acataatcag taagctcgaa ccaccgtaac tgatcagcgg caatgtcaga cctttggtcg
    22141 gtaacatccc cgccgccgcg cctacgttaa ccagcgcctg gaagctaaac cagatgccaa
    22201 tagaacaggc gagaaaaccg gaaaaacggt ggtcaatttc taacgctttg cggccaatcg
    22261 acatcgcgcg aaaagcgacg aagaatacca ttaaaagtgc cagcaccaca ccgacatacc
    22321 ccagttcttc gccgataatg gcgaaaataa agtcagtgtg cgcttccggc agatactcca
    22381 gtttttgtac cgagttacct aaaccttgtc cccaaagttc gccgcgacca aacgccatca
    22441 gcgattgcgt taactgatag ccgctgccaa agggatcttc ccacgggttc cagaatgcgg
    22501 taacacggcg gatacggtac ggttcggcga gtatcagcaa cacaaccgct gaaatgccca
    22561 taccgataat ggcaatgaac tgccacaatt tcgctcccgc caggaacaac atcgccagcg
    22621 tagtcacaaa caacaccacc accgtaccaa ggtctggctg tgccagcagt aacactgcca
    22681 acaccagaat cacgcccatc ggtttcagga agccgcgcag gttattacgt acttcgtcgc
    22741 ctttacgcac cagatagttg gcgatataac aaaacagcga cagttttgtc agctccgcag
    22801 gctggatacg cagcaaaccg agatcgatcc aacgcgatgc ccctttaacc gagctaccca
    22861 ctaccagcac gatcatcagc aggatgatag atccgagcag catcgtggca ctgtagcgtt
    22921 gccagaactc catcggcaga cgcagcgtaa tgatcgccag aatgaatgcc aggatcagat
    22981 agacgccatc acgcttcgcg aagaagaacg gatcgttggt taagcgttgc cctatgggca
    23041 ttgacgccga ggtcaccatg ataaagccaa tcgccgcaag gccgaaggtc agccacagta
    23101 aggtgcgatc gtacatgatc aggctgtcgg tatctttttc ccgcgagccc atcacccagc
    23161 cttttagcgc cgtggagatc cagaccagga tactgaatcc tggcaggcgc ggcattttca
    23221 ggcgagggag agataaacgc atcaacctaa ctccttcgcc agacgggcaa actcattgcc
    23281 tcgttgttca aagttcttga actgatcaag gctggcacag gccggggaga gcagaaccat
    23341 atcgcccagc tgaacacgcg gagccagcaa gcgcatcgct tgttccatgg tttcggtttg
    23401 ttctgccact tccgggcgta gcgccgccag ctgcgcaccg tcacgaccaa aacagtacaa
    23461 acgcacgttg tcgccattca gatagcgcgc cagtggactg aagtccgccg atttaccatc
    23521 gccgcccagc aacaaatgca gcgtgccgtc tacctgcaag ccattcaacg ccgcttccgt
    23581 actgccgacg ttagtggctt tcgaatcgtt aatccagcgt acgccattgt gctccagcac
    23641 cacttcaaag cgatgcggca gaccggtgaa ggtggttaac gctttcaggc tgctggcgcg
    23701 cggcaaacca gctgcgtctg ccagtgccag tgctgccagc gcattggtgt agttatgctg
    23761 cccggaaagt ttcatctctt tcacattcag cactttctcg cctttaaccc gcagccaggt
    23821 ttcgccctgc tgatgattca ggtgatagtc acccatgttg acgccaaagc tgacgcagcg
    23881 ttcatcagca ccgcgaatcg gcattgttaa ggcatcatca gcattgacga cacaaacttt
    23941 cgcattttcg taaatacgca attttgccgc acggtattgc tgcaaaccaa acggatagcg
    24001 gtccatatga tcttcagtca cgttcagaat ggtcgccgcc accgcctgta agctggaagt
    24061 ggtttccagc tggaagctcg acagttccag cacgtacagt tcgcactcag catcaagcag
    24121 catcaacgct ggcaggccaa tattgccacc cacaccaacg ttaacccccg ccgctttcgc
    24181 catttcaccc actagcgtgg tgaccgtgct tttgccgtta gaaccggtaa tcgccacaat
    24241 cggtgcttgt gcttcgcgac agaacagctc gatatcgcca acgatttcga ttccggcatc
    24301 agcggcagcg cttaaggatg gatgcgcctg tgcaataccg ggactggcga caatcagatc
    24361 tgccgccatc agccattcat cattcagact gcccgtgtgg cgttctacgg cttcgggtaa
    24421 tttatccagg ccaggcggtg tcatacgcgt atccataacg cgcggcgtca caccgcgagc
    24481 gaggaaaaag tccacgcagg aaagcccggt gaggcccagg ccgataatga cgacattttt
    24541 accctgataa tcagccatga ttaacgtacc ttcagcgttg ccagaccaat cagaaccagc
    24601 atcagcgaaa taatccagaa acgcacaatg acgcgcggtt ccggccagcc tttcagttca
    24661 tagtggtgat gaatcggtgc catgcggaaa atacgttgtc cgcgcagttt aaaggagccg
    24721 acctgcagga tgacagaaag cgtttctacc acgaacacgc cccccataat caccagcagg
    24781 aattcctgac gtagcagtac ggcgataatg cctaacgcac cacctaacgc cagcgaacct
    24841 acatcgccca taaagacctg cgccggatag gtgttaaacc acaggaagcc cagtcctgcc
    24901 ccgactatcg ccgtgcagac aatgaccagt tcccctgcgt gtcgcagata cggtatatgc
    24961 aagtagctgg caaagttcat attgccggtc gcccacgcca ccagcgcaaa accaccggcg
    25021 acaaatacgg tcggcataat tgccaggccg tcgagaccat cggtcaggtt taccgcgttg
    25081 ccagtaccca caatgacgaa gtaagccagc agaatgtaga acagccccag ctgcggcatc
    25141 acatctttaa agaatgggac caccagctgc gttgcgggcg tgtctttgcc ggcaaggtac
    25201 agggcgaagg cgacacccag cgcaatgacc gacatccaga aatacttcca acgagcgatc
    25261 aaccctttgg tgtctttacg caccactttg cgataatcat caacaaagcc aataacaccg
    25321 taacctacca gcaccaccaa cacgcaccag acgtacggat tggacgggta agcccacagc
    25381 agtacggaga tcacaatcgc cgtcaggatc ataatcccgc ccatggtcgg cgtaccgcgc
    25441 ttgctgaagt gtgattcagg accgtcgtta cgcaccacct gaccaaagga aagtttttgc
    25501 aaatgagcta tcatacgcgg gcccatccac aatgagatga acagcgcggt cagcaggctg
    25561 acgatggcgc gaaacgtcag ataggaaaag acgttaaagc cggaataata tttgaccaaa
    25621 tgttcggcca gccaaactaa catgtcccat tctcctgtaa agcgcgtact acctcttcca
    25681 tggcggcact acgtgaaccc ttaactaaaa tcgtaattac ctgttgctca gcaatcagtg
    25741 atttaagacg cgtaattaac gcagttttat cagcaaaatg ttcgccaacg ccgctggcgg
    25801 tgctgatagc atggctttgt ttacccacgc ttaacacgcg gtcaatacca gcagcttttg
    25861 ccgcctcgcc cacctgtaca tggcaggctt cgctttcagc gcccagttcc gccatatcgc
    25921 ccaccaccag cacgcggtag cccggcattt cagccagtac ctgaacagcg gcggtcattg
    25981 aaccgacatt ggcgttgtag gagtcgtcga gcagcaactg gttttctgcc agttggatgg
    26041 ggaacagacg gcctggaaca gctttcagat ttgccagccc cgctttgata gcatcaagcg
    26101 ttgcgcccac ggacatggag agcgcagcgg ctgccagcgc attcgcaata ttgtgacgcc
    26161 ccggcaacgg cagcagaaca tcgacgctac cggttggggt ttgtagggta aattccgtac
    26221 cgtgcgaggt cacatggata ttggtggcgg tgaaatcgct gttggcggca ttgggtgaga
    26281 aacgccacac tttgcgtgag ccaattacgc tctgccagtt cagccagtcg ttgttgtcgg
    26341 cgttcataat ggcgataccg ttttccggca ggccgctaaa gatttcacct ttcgctttcg
    26401 cgacacccgc aagcgagcca aaaccttcca gatgcgccgc tgccaggttg ttgaccagcg
    26461 cagcttccgg gcgagtcaga ctcacagtcc aggctatttc gccctgatgg ttcgcgccaa
    26521 gttcaataac tgcgtaatcg tattccggcg tcaagcgcaa caacgtcatc ggtacaccga
    26581 tgtcgttgtt gagattgcct gccgtataaa gcgtgttgcc gcactggctt aaaatcgccg
    26641 ccgtcatctc tttaacggag gttttgccgg aggaacctgt cagagcaacc acgcgcgccg
    26701 gaacttgctg gcgaacccat gcagccagtt caccaaacgc cagacgcgta tccttgacga
    26761 ttaactgcgg caggtcgata tccagcggac ggctgaccag tagtgcgccc gcgccgccag
    26821 ctttcgcctg gtcggcaaaa tcatgagcat cgaaacgttc gcctttcagg gcaacaaaca
    26881 ggcagcccgg cgtcagtttt cgggtatcag tggttacagc atcaagggtg atatctgcac
    26941 cttgcagttc accgttgaga atgtcggtaa gttggctaag ggttacgcta atcatgcaat
    27001 cacccccagc agacgcgcca ccgtgacgcg atcggagtag tccagacgct gattgccaac
    27061 aatctggtaa tcttcatggc ctttgcccgc gaccagtacc acatcattct ctttagcctg
    27121 cataacggcg caagtcaccg cttcagcacg gccttccatc actttggcat gtccggcatc
    27181 taacattccc gccagaatat cgttgatgat ggcacgcggt tcttcggtac gcgggttatc
    27241 gtccgtcacc accgccacgt cagcaaactc ttcggcaatt gcgcccatca gtggacgctt
    27301 acctttatcg cgatcgccac cacagccaaa gacgcaccac agcttgcccg cacagtgcag
    27361 gcgcgccgcc tgtaaggctt tttccagtgc atccggcgta tgcgcgtaat ccaccaccac
    27421 cgtcggtttg cctggcgcag tgaacacttc catacgtccg caaaccggtt gcagacgcgc
    27481 ggcggttttc agcagatcag ccagtggata gccgagtgcc agcagtgtcg ccagcgcgag
    27541 cagcaggttg ctgacgttaa aagcgcccat cagatggctt tcaatttcgc catcgcccca
    27601 acttgagcta aagcgaatcg tcgcaccgct gtcgtgatag ttcacgtcga tcgcttttaa
    27661 ccagcgtccg tgacagttcg gattaatatg atcttccatt gataccgcaa ccgcgtccgg
    27721 cagttttgcc agccagcggc ggcccacttc atcgtcggcg ttaataatcg cctgaccgca
    27781 atgatgttca gaataaagca gccatttcgc agcttcgtag tgttccatat caccatgata
    27841 atcaaggtga tcgcggctta agttggtaaa gaccgacgcc gcaaatttca atgccgccac
    27901 acggtgctgt accagcccgt gggaggaaac ttccattgcg caaaacgtcg cgccctgctc
    27961 caccagcccc gccagctcat gctgaacatc gactgcggaa ccggtagtat tttctgtcgg
    28021 gatcactttc ccaagcagac cgttaccaac ggtgcccatt accgcgctgg tttcgccaag
    28081 cagttggctc cactgcgcca gcagctgggt agtcgtggtt ttgccgttgg tgcccgttac
    28141 gcctacgaga cgtaaattgt cagagggttc atggtaaaag cggcccgcca gtgcagataa
    28201 acgctcattg agctggctga gatagatgac cggtacgccg tgcatttcac ggatttcacc
    28261 gtcggtcgcc tcatctttcg ctttcgcctc tgcaataatg gcagccacac cttgcgctat
    28321 cgcctgcggg atatatcgac gcccgtccgc ctgatgacct actacagcta caaagagatc
    28381 gcccgccgca gccacacggc tgtcgagtgt catctctcgc agtgctcgcg aaggtgcgtc
    28441 tggcacccac ggagcaagaa ggtcgcgcaa attacgatct gccacctgtc ccctcgcctt
    28501 gattaatcac aaattcattt ttatcgcccg ttgtcagcgc atccggctcg atgttcatgg
    28561 tacgcaatac gccgcccatg atggcaccaa agaccggcgc ggaaacggcg ccgccgtagt
    28621 atttacccgc ctgcggatcg ttgataacaa caaccagcgc gaagcgcggc tgactcgcag
    28681 gcgcaacgcc tgcggtataa gcaatatatt tattgatgta gcgaccgtcc ggcccgacct
    28741 ttttcgcggt accggtttta atggcgatac gatagccttt aatcgccgcc ttcacgccgc
    28801 cgccgcctgg tagcgccacg ctttccatca tatgcaccac agtgcggaca atggatcccg
    28861 ggaagacacg ttcaccggga accggggggt caactttggt aatcgacagt gggcgataaa
    28921 tgccgtagct gccgatagtt gcgtagactc gcgctaactg taacggtgtt accattagcc
    28981 cgtagccgaa agagaaggtg gccctctcta tgtcagacca ccgttgtttt tgaggatata
    29041 agccactgcg ttctccgacc aaccccaaat tggtcgcttt tcccagtcca aaacgtgagt
    29101 aagtatctac taacgctgag gacggcatcg ctaacgccag cttggaaaca ccgacgttac
    29161 tcgacttctg taataccccg gtcagggtta attcgctgta gcgtgccacg tctttgattt
    29221 cgtggccgtt aattcgataa ggaatggtat tgagtaccga gttttcccgc accacgccac
    29281 gttgcaacgc ggtcattacc accatcggtt taaccgttga gcccggttca aacacgtcgg
    29341 tgatggtacg gttacgcatc gcctctttcg gcgtgccgct cagattgtta gggttgtatg
    29401 acgggctgtt agccatcgcc agcacttcac cggtgttgac atccaccagc acggcgctac
    29461 cagattcagc cttgttaaag gccaccgcgt tgttcagttc gcgataaacc agcgcctgca
    29521 ggcgttcatc aatactcagc gccaggttgt gcgctgcctg gctgtcagta gaagaaatat
    29581 cttcaattac gcgaccatag cggtctttac gcacaatgcg ctcacccggc tgcccggtaa
    29641 gccatttatc gaaactcttc tcaacgccct caatcccttg actatcgacg ttagtaaagc
    29701 cgatgaggtg agcagtcact tcgccggacg gatagtaacg gcgagactct tcacgcagat
    29761 gaatccccgg cagtttcagt tttttgatgt agtccgccat gtcagggttc acctgacgcg
    29821 ccagataaat aaaacgccct ttcgggttgg cgttaatgcg ggctgaaagc tgatccagcg
    29881 gaatattgag cgcgttagcc agcgccttcc agcggtcacc gacgctgata ccgccagcgt
    29941 catgcacttc tttcgggtca gcccaaatcg cttttaccgg cacgctcacc gctaacgggc
    30001 gaccagaacg gtcagtaatc atgccgcggg aggtggaaac ttgctgaacg cgaagagaac
    30061 gcatgtcgcc ctctttcacc agcatatccg gggagataac ttgtaaccac gctacgcgtc
    30121 cgagcagaaa agccagcgcc aggagaatac agccgcataa caacgcaaaa cgccaactga
    30181 taaagttggc atgttcttcc tgacgttttg gtttctgcgt tttcgccgct gctttcatgc
    30241 gtcgcgttta tccttatttt tgcactacga tattttcttg tgacggatca acatgctgca
    30301 tttgcagctt ttccgtggcg atcctttcca cccggctatg gtcgccgagc gcattctctt
    30361 caaggatcag gttgcgccat tcaatgtcta aagcatctcg ctccagcacc agttgttcgc
    30421 gctgagcggt cagtaaacgg gtatggtgcg ccgtggttac cacagtcacc gccgtcaaaa
    30481 taatgcaaat gaacaggcag agtggcagct tcccaaatcg caaaagatcg tcaccgataa
    30541 caccaggcaa tgcatggcgc tcgtggcttc ccatcgatcc tttaactttg cttagagctt
    30601 ctgtcactct gctgatcatg cattcgtcct ctctgcaata cgcagaactg aactacgggc
    30661 acgagggttc tcagccacct cttcttcgcc cggcattaac ttgcctagtg ctcgcagctg
    30721 acggccaccc agttttttga gctgctcttc agtcatcggt aaccctgccg gaacttgcgg
    30781 accgcggctg ttttcacgca taaaacgttt cacaatacgg tcttccagcg agtggaagct
    30841 gatgatcgaa agccgcccac ccggggccag cacgttgagc gagcttttta gcgcctgctc
    30901 tatctcctcc agttcactgt ttacccaaat gcgcaccgcc tggaaggtac gggtcgcggg
    30961 atgtttaaac ttatctttca ccggcgttgc aacagccacg acttccgcca gttctttggt
    31021 gcgggtcatc ggctgttcgc ggttacgctc gacaatggcg cgggcaatgc gtttggcaaa
    31081 acgctcttca ccataggttt tcaataccca agcgatatcg gcttcttctg cggtttgtag
    31141 ccattcagcg gctgactgcc cacgggttgg gtccatacgc atgtccagcg gaccatcgcg
    31201 cataaaggaa aagccgcgct cagcatcatc aagttgcggt gaagagacgc caagatcgag
    31261 gagaatgccg tcgatcttgc cgataagatc gcgctcggca acgtattcgc ccagcgcgga
    31321 gaaaggtccg tggatgatgg agaagcgcgg atcatcaata gtcttcgcaa cggcgatagc
    31381 ctgcgggtcg cgatcgatcg ccagcaaacg cccctcttcg ccaagctgcg agaggatcag
    31441 acgtgagtga ccaccgcgac caaaagtccc atcaatgtag atgccatcag gacggatatt
    31501 gaggccatta acggcttcat ccagcagcac cgtagtatgt ttatagtttt ccatcatttt
    31561 atagagacaa gtcctgcagt cgctccgata agtctccggt agccaactgc tctgcgtcga
    31621 tatcttcctt gacctgttga tgccaggttg tttcatccca cagctcaaac ttgttgaact
    31681 gtccaaccag catcacttct tttgtcagcc cggcatgttg ccgcagtact ggcgcgatta
    31741 acaatcgacc tgcgccatcc atctgacatt cgctggcatg acctaacagt aggcgctgca
    31801 cacggcgctc aaccgggttc atgctcgaca gacgcgataa tttttgctcg ataatttccc
    31861 attcaggcag ggggtaaagc agcaggcacg ggtgatgaat gtcaatggtg caaaccattt
    31921 gaccgacagc gttctcaagc agctgttccc ggtaacgggt aggcactgat aagcgccctt
    31981 tgctgtcgag attgactaac gttgctcccc ggaacatgcc agcctcaccc cttattccca
    32041 ctttgcccca caatttccca ctgaaaggag tttacggagc ggaggaaaag cttgtcaagc
    32101 cagtcacaag gcataccgag gtaaaaaacc cgatgtttac ggctaatatc agcgttgttt
    32161 attttacgtc caacgtgcgc agcaaattaa cgttatgaat agctgtaaga aaaaatccct
    32221 catcgtcatc accgttttaa aatgactcac tattttccac agcaaatata aagtgtcagt
    32281 ttgcgacgcg agcggcattt taggacatat cttccccggt taacagtccc tgttgcgtct
    32341 gtgtggcgct tgatgccagc gggtttgcgc aaaagcaaag aaaagtgctt gttaaatcgg
    32401 caattcatct ctgcatgtaa caaaataatg caaaaacgcg ctgtcgatat cacttaatta
    32461 ttgtgaattt cagctggtga gttaattaac gggcgggttt aaaagaactt atcaataata
    32521 gtcaggaata gtcttattta ctttaaggat attgatgtcc attcccgtac tctacg
//
LOCUS       DAZPJR010000032        32533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPJR010000032 DAZPJR010000000
VERSION     DAZPJR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.033"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..94
                     /locus_tag="ACHVB7_001376"
     CDS             <1..94
                     /locus_tag="ACHVB7_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1913403.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="ACHVB7_001377"
     CDS             complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="ACHVB7_001377"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1913404.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="ACHVB7_001378"
     CDS             complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="ACHVB7_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR1913405.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2848..4476
                     /gene="glpA"
                     /locus_tag="ACHVB7_001379"
     CDS             2848..4476
                     /gene="glpA"
                     /locus_tag="ACHVB7_001379"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR1913406.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4466..5725
                     /gene="glpB"
                     /locus_tag="ACHVB7_001380"
     CDS             4466..5725
                     /gene="glpB"
                     /locus_tag="ACHVB7_001380"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR1913407.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5722..6912
                     /gene="glpC"
                     /locus_tag="ACHVB7_001381"
     CDS             5722..6912
                     /gene="glpC"
                     /locus_tag="ACHVB7_001381"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR1913408.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7105..8007
                     /locus_tag="ACHVB7_001382"
     CDS             7105..8007
                     /locus_tag="ACHVB7_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1913409.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="ACHVB7_001383"
     CDS             complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="ACHVB7_001383"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR1913410.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8869..10158)
                     /locus_tag="ACHVB7_001384"
     CDS             complement(8869..10158)
                     /locus_tag="ACHVB7_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1913411.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="ACHVB7_001385"
                     /pseudo
     CDS             complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="ACHVB7_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11434..12216)
                     /locus_tag="ACHVB7_001386"
     CDS             complement(11434..12216)
                     /locus_tag="ACHVB7_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR1913412.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12436..13638)
                     /locus_tag="ACHVB7_001387"
     CDS             complement(12436..13638)
                     /locus_tag="ACHVB7_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR1913413.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13738..14280)
                     /locus_tag="ACHVB7_001388"
     CDS             complement(13738..14280)
                     /locus_tag="ACHVB7_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR1913414.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14559..14984
                     /gene="nudI"
                     /locus_tag="ACHVB7_001389"
     CDS             14559..14984
                     /gene="nudI"
                     /locus_tag="ACHVB7_001389"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR1913415.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15023..15625)
                     /gene="ais"
                     /locus_tag="ACHVB7_001390"
     CDS             complement(15023..15625)
                     /gene="ais"
                     /locus_tag="ACHVB7_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR1913416.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15933..17072
                     /gene="arnB"
                     /locus_tag="ACHVB7_001391"
     CDS             15933..17072
                     /gene="arnB"
                     /locus_tag="ACHVB7_001391"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR1913417.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17076..18044
                     /gene="arnC"
                     /locus_tag="ACHVB7_001392"
     CDS             17076..18044
                     /gene="arnC"
                     /locus_tag="ACHVB7_001392"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR1913418.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18044..20026
                     /gene="arnA"
                     /locus_tag="ACHVB7_001393"
     CDS             18044..20026
                     /gene="arnA"
                     /locus_tag="ACHVB7_001393"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR1913419.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20023..20913
                     /gene="arnD"
                     /locus_tag="ACHVB7_001394"
     CDS             20023..20913
                     /gene="arnD"
                     /locus_tag="ACHVB7_001394"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR1913420.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20913..22565
                     /gene="arnT"
                     /locus_tag="ACHVB7_001395"
     CDS             20913..22565
                     /gene="arnT"
                     /locus_tag="ACHVB7_001395"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR1913421.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22562..22897
                     /gene="arnE"
                     /locus_tag="ACHVB7_001396"
     CDS             22562..22897
                     /gene="arnE"
                     /locus_tag="ACHVB7_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR1913422.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22897..23283
                     /gene="arnF"
                     /locus_tag="ACHVB7_001397"
     CDS             22897..23283
                     /gene="arnF"
                     /locus_tag="ACHVB7_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR1913423.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="ACHVB7_001398"
     CDS             complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="ACHVB7_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR1913424.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23587..25008)
                     /gene="menE"
                     /locus_tag="ACHVB7_001399"
     CDS             complement(23587..25008)
                     /gene="menE"
                     /locus_tag="ACHVB7_001399"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR1913425.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25005..25967)
                     /gene="menC"
                     /locus_tag="ACHVB7_001400"
     CDS             complement(25005..25967)
                     /gene="menC"
                     /locus_tag="ACHVB7_001400"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR1913426.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAEMADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25967..26824)
                     /gene="menB"
                     /locus_tag="ACHVB7_001401"
     CDS             complement(25967..26824)
                     /gene="menB"
                     /locus_tag="ACHVB7_001401"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR1913427.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26839..27597)
                     /gene="menH"
                     /locus_tag="ACHVB7_001402"
     CDS             complement(26839..27597)
                     /gene="menH"
                     /locus_tag="ACHVB7_001402"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR1913428.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27594..29264)
                     /gene="menD"
                     /locus_tag="ACHVB7_001403"
     CDS             complement(27594..29264)
                     /gene="menD"
                     /locus_tag="ACHVB7_001403"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR1913429.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29353..30648)
                     /gene="menF"
                     /locus_tag="ACHVB7_001404"
     CDS             complement(29353..30648)
                     /gene="menF"
                     /locus_tag="ACHVB7_001404"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR1913430.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="ACHVB7_001405"
     CDS             complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="ACHVB7_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR1913431.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31087..31530)
                     /locus_tag="ACHVB7_001406"
     CDS             complement(31087..31530)
                     /locus_tag="ACHVB7_001406"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1913432.1"
                     /translation="MHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDLTGDNRH
                     ILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDTCTHHWP
                     DKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            31613..32530
                     /gene="rbn"
                     /locus_tag="ACHVB7_001407"
     CDS             31613..32530
                     /gene="rbn"
                     /locus_tag="ACHVB7_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR1913433.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7896 a         8806 c         8138 g         7693 t
ORIGIN      
        1 ttcgttgttg gctaaagaaa agccggtaga aggcagtcca ctgtttttac tacaaatgtg
       61 catggaacaa ttagtggcga ttggaaaaat ttgatatgag acaggatggc gcatgaacgc
      121 catcctgcag tagcattact ctttattaag gaattttact gccttatcag ggaaatcagt
      181 aaacagccca tttacacccg ctttgttata cagagcatca tataactgat tcacatcagt
      241 agtgtattca ggcagtttat ccgaccgcac ggtataagga tgcactacca gtttattctg
      301 ctgagcatct tgcaccatgc cagtgagttt gatattaccc ggctgcgatg tttcctcaat
      361 caacatatgg taatccggcc cgataccgtc ggcatattcc gcgacttgct tcatggcacc
      421 tggcttaaac atccagtcat agctgtaatt aacccagctt ccgtccggct gtttctgctg
      481 cgtttcattc cagtcggtat aggcaatcag ctgtaccaga ttgagatcca tgcccatttt
      541 aggttccagc tcattcttaa tacgcttcag ctcatcagca tcaaaacatt gcaaataaac
      601 tttatcgtcc ttaccggtgt aaccatattt cttcagcact tccagcgttt ttgccgcaat
      661 atccttccct tcctgatgat ggaaccacgg cgctttgatt tctggataga taccgatatt
      721 tttcccggta gagtgattta acccctgaac aaattcaatc tcttcttcaa aggtgtgccc
      781 ccggaagtcg gacttaccca ttgggaaacg ccccggataa gtctgtactt ttttaccgtt
      841 ttcaatatcg aaaccttcgg taaatttcag cgacttaatt tcatccagcg tgaaatctat
      901 cgcgtagtaa cgaccgtctt tgcgcgcccg atccgggaaa cgatcggcaa catcagtaac
      961 acgatcgaga taatggtcat gcagaacaac cagatggtcg tctttggtca tcaccaaatc
     1021 ctgttccaga taatccgctc cctgcgcata cgccatcgct tttgctggca gcgtatgctc
     1081 cggcaaatat ccactggcac cgcgatgggc gatgactatt ttttcgttgc tgtccgccgc
     1141 cattgcactg cttcccatga ctatagtgct catcatgatc gccatgctaa ggtttttcag
     1201 cttcaatttc atgccattag cctccgttgc gtttttgcag taattgttca tggcgacgtt
     1261 tttcgccaat catcacaaca atcaacaaga taaccgccag aatgctgccg ccaatcatta
     1321 ccataaagcc gccatcccag ccgaagaagt ccacggtgta gccaacaatc gcgctcgccg
     1381 ccaccgaacc gcccaggtaa ccaaacagcc cggtaaagcc cgctgccgta cctgccgctt
     1441 ttttcggtgc cagttccagc gcatgcagac cgatcagcat cacaggaccg tagatcagga
     1501 agccgataac aatcatacaa atcatatcga cggttgggtt acctgccggg ttcatccagt
     1561 aaacgatagt cgcgatggtc accagtgtca taaagaaaac gccggttgcc ccacggttgc
     1621 cacggaagac tttatccgac atccagccgc acagcagagt gcccggaata cctgcatatt
     1681 cataaaggaa gtaggcccag gaggatttat ccagcgcgaa atgcttaacc tctttcagat
     1741 aagtcggtga ccagtcgagg atgccgtaac gcagcagata aacgaacacg ttggcgatgg
     1801 cgatatacca cagcagtttg ttcggcagta cgtactgcat gaagatttgc ttcgccgtca
     1861 gctcctgttc cgctttttcg ttatagtcgt ccggataatc atttttgtac tcttcgatcg
     1921 gcggcaagcc acaggattgc ggggtatcgc gcatcatcgc aaaggcgaat aatgccacca
     1981 gaatggcgca gaaagcaggc atatagagcg ccgcatgcca gtcattgaac caggccatcc
     2041 ccagcaggaa cagcagcggc ggaataccac caccgacgtt gtgcgcacag ttccacactg
     2101 acacaatgcc gccacgttct ttctgcgacc accagtgcac catagtacga ccacacggcg
     2161 gccaccccat cccctggaac caaccgcaga ggaacaacag tacaaacatc accgcaatgc
     2221 tcgacgtcgc ccatggcaca aagcccataa acaacatcac tgccgccgcc agaatcaaac
     2281 ctgcgggcag gaaaacgcgc ggattcgagc gatccgatac cgaacccatg atgaatttcg
     2341 aaaatccata agcaatcgag atccccgaaa gggcaaaacc taaatcaccg cgtgagaatc
     2401 cctgctcaac cagataaggc atagcaagcg caaagttctt acgaaccaaa tagtaagccg
     2461 catagccaaa gaatatcccc aggaaaattt gccagcgcaa tcgacgataa gtcggatcga
     2521 tctccgcggc aggtaagcgc gctttgtgtg gcgctggttt aaaaatactc aacattgata
     2581 gcctccgtgg cccgtggtct tatttatgat taacagcctg attcagtgag agaacctgcc
     2641 gtttcttgag ttgccgcgat gttaagaaaa cattcataaa ttaaatgtga attgccgcac
     2701 acattattaa ataagattta caaaatgttc aaaatgacgc atgaaatcac gcttcacttt
     2761 cgaatcatga gcgaatatgc gcgaaatcaa acaattcatg tttttactat ggctaaatgg
     2821 taaaaaacga acttcagagg gataacaatg aaaactcgcg actcgcaatc aagtgacgtg
     2881 attatcattg gcggcggcgc aacgggagcc gggattgccc gcgactgtgc cctgcgcggg
     2941 ctgcgcgtga ttttggttga gcgccacgac atcgcaaccg gtgccaccgg gcgtaaccac
     3001 ggcctgctac acagcggtgc gcgctatgcg gtaaccgatg cggaatcggc ccgcgaatgc
     3061 attagtgaaa accagatcct gaaacgcatt gcacgtcact gcgttgaacc aaccaacggc
     3121 ctgtttatta ccctgccgga agatgacctc tccttccagg ccacttttat tcgcgcctgc
     3181 gaagaagcag ggatcagcgc agaagctata gacccgcagc aagcgcgcat tatcgaacct
     3241 gccgttaacc cggcattgat tggcgcggtg aaagttccgg atggcaccgt tgatccattt
     3301 cgtctgaccg cagcaaacat gctggatgcc aaagaacacg gtgccgttat ccttaccgct
     3361 catgaagtca cgggactgat tcgtgaaggc gcgacggtgt gcggtgttcg tgtacgtaac
     3421 catctcaccg gcgaaactca ggcccttcat gcgcctgtcg tggttaatgc cgccgggatc
     3481 tgggggcaac acattgccga atatgccgat ctgcgcattc gcatgttccc ggcgaaagga
     3541 tcgctgctga tcatggatca ccgcattaac cagcatgtga tcaaccgctg ccgtaaacct
     3601 tccgacgccg atattctggt gcctggcgat accatttcgc tgattggcac cacctcttta
     3661 cgtattgatt acaacgagat tgacgataat cgagtgacgg cagaagaggt tgatattctg
     3721 ctgcgtgaag gggaaaaact ggcccccgtg atggcgaaaa cgcgcatttt gcgggcctat
     3781 tctggcgtgc gcccgctggt tgccagcgat gacgacccga gcggacgtaa cgtcagccgt
     3841 ggcatcgtgc tgctcgacca tgctgaacgc gatggtctgg acggatttat caccatcacc
     3901 ggtggcaaac tgatgaccta tcggctgatg gctgaatggg ctaccgacgc cgtgtgccgc
     3961 aaactgggca acacgcgccc ctgtacgacc gccgatctgg cacttcctgg ttcacaggaa
     4021 cccgctgaag ttaccttgcg taaagtcatc tccctgcctg ccccgctgcg cggttctgcg
     4081 gtttatcgtc atggcgatcg cacgcctgcc tggctgagcg aaggtcgtct gcaccgtagc
     4141 ctggtatgtg agtgcgaagc ggtaacagcg ggtgaagtgc agtacgcggt agaaaattta
     4201 aacgttaata gcctgctgga tttacgccgt cgtacccgtg tggggatggg tacctgccag
     4261 ggcgaactct gcgcctgccg cgctgccgga ctgctgcaac gttttaacgt cacgacgtcc
     4321 gcgcaatcta tcgagcaact ttccaccttc cttaacgaac gctggaaagg cgtgcaaccc
     4381 atcgcctggg gagatgcact gcgcgaaagc gaatttaccc gctgggttta tcagggactg
     4441 tgtggtctgg agaaggagca gaaagatgcg ctttgatact gtcattatgg gcggcggcct
     4501 cgccggatta ctctgtggcc tgcaactgca aaaacacggc ctgcgctgtg ccattgtcac
     4561 gcgtggtcaa agcgcgctgc atttctcatc cggttcgctg gatttgctga gccatctgcc
     4621 tgatggtcaa ccggtgacag acattcacag tggactggaa tctttgcgtc agcaggcacc
     4681 agcccatcct tacagtcttc tcgaaccaca acgcgtgctc gatctcgctt gccaggcgca
     4741 ggcattgatc gctgaaagcg gtgcgcaatt gcagggcagc gtggaactgg cccatcagcg
     4801 cgttaccccg ctcggcactt tgcgtgcaac ctggttaagt tcgccagaag ttcccgtctg
     4861 gccgctaccc gcgaagaaga tatgtgtagt ggggataagc gggctgatgg atttccaggc
     4921 gcatcttgcg gcggcttcgt tgcgcgaact ggatctttcg gttgaaaccg cagaaataga
     4981 gctgccggaa ctggatgtcc tgcgcaataa cgccaccgaa tttcgcgcgg tgaatatcgc
     5041 ccgtttcctt gataatgaag aaaactggcc gctgttactt gatgcgctta ttcctgtcgc
     5101 caatacctgc gaaatgatcc tgatgcccgc ctgcttcggc ctggccgatg acaaactgtg
     5161 gcgttggttg aatgaaaaac taccttgttc actgatgctt ttgccaacgc tgccgccttc
     5221 cgtgctgggc attcgtctgc aaaaccagtt acagcgccag tttgtgcgcc agggtggcgt
     5281 gtggatgccg ggcgatgaag tgaaaaaagt gacctgtaaa aatggcgtag tgaatgaaat
     5341 ctggacccgc aatcacgccg atattccgct acgtccacgt ttcgcggttc tcgccagcgg
     5401 cagtttcttt agtggcggac tggtagcgga acgtaacggc attcgcgagc cgattctcgg
     5461 ccttgatgtg ctacaaaccg ccacgcgggg tgaatggtat aagggcgatt tttttgcgcc
     5521 gcaaccgtgg cagcagttcg gtgtaaccac tgatgagacg ctacgcccgt cgcaggcagg
     5581 gcaaaccatt gagaacctgt ttgccatcgg ttcggtgctg ggcggatttg atcccatcgc
     5641 ccaaggatgc ggcggcggtg tttgtgccgt cagtgcttta catgccgctc aacagattgc
     5701 ccaacgcgca ggaggccaac aatgaacgac accagcttcg aaaactgcat taagtgcacc
     5761 gtctgcacca ccgcctgccc ggtgagccgg gtgaatcccg gttatccagg gccaaaacaa
     5821 gccgggccgg atggcgagcg tctgcgtttg aaagatggcg cactgtatga cgaggcgctg
     5881 aaatattgca tcaactgcaa acgttgtgaa gtcgcctgcc cgtccgatgt gaagattggc
     5941 gatattatcc agcgcgcgcg ggcaaaatat gacaccacgc gcccgtcgct gcgtaatttt
     6001 gtgttgagtc ataccgacct gatgggtagc gtttccacgc cgttcgcacc aatcgtcaac
     6061 accgctacct cgctgaaacc ggtgcggcag ctgcttgatg cggcgttaaa aatcgatcat
     6121 cgtcgcacgc taccgaaata ctccttcggc acgttccgtc gctggtatcg cagcatagcg
     6181 gctcagcaag cacaatataa agaccaggtc gctttctttc acggctgctt cgttaactac
     6241 aaccatccgc agttaggtaa agatttaatt aaagtgctca acgcaatggg tatcggtgta
     6301 caactgctca gcaaagaaaa atgctgcggc gtaccgctaa tcgccaacgg ctttaccgat
     6361 aaagcacgca aacaggcaat tacgaatgta gagtcgatcc gcgaagctgt gggagtaaaa
     6421 ggcattccgg tgattgccac ctcctcaacc tgtacatttg ccctgcgcga cgaatacccg
     6481 gaagtgctga atgtcgacaa caaaggcttg cgcgatcata tcgaactggc aacccgctgg
     6541 ctgtggcgca agctggacga aggcaaaacg ttaccgctga aaccgctgcc gctgaaagtg
     6601 gtttatcaca ctccgtgcca tatggaaaaa atgggctgga cgctctacac cctggagctg
     6661 ttgcgtaaaa tcccggggct tgagttaacg gtgctggatt cccagtgctg cggtattgcg
     6721 ggtacttacg gtttcaaaaa agagaactac cccacctcac aagccatcgg cgcaccactg
     6781 ttccgccaga tagaagaaag cggcgcagat ctggtggtca ccgactgcga aacctgtaaa
     6841 tggcagattg agatgtccac aagtcttcgc tgcgaacatc cgattacgct actggcccag
     6901 gcgctggctt aaactccttt ctgatgcccg gtaagcatgt ggttaccggg catttttgcg
     6961 tacacgattc cgtgcccaat gtatgcgttg caacgcagtg aaaattcctc tgaaaacgtc
     7021 tcgcaaaggc tgaaactggc agatgtcaaa ggcctgggat aaccgtaatg tcgcgtcatc
     7081 ataaatatca ggtgacggac aaccatgacc gaatcaacaa cctcctcccc gcatgatgcg
     7141 gtatttaaaa cctttatgtt cacacccgaa accgcacggg attttctcga aatacatttg
     7201 ccagaaccac tgcgcaagct ttgcaacctg caaaccttac gcctggaacc cactagtttt
     7261 attgaaaaaa gtttacgcgc ttactactcg gatgttttgt ggtccgtgga aaccagcgac
     7321 ggtgacggct atatctactg cgtgattgaa catcaaagct ctgcagaaaa gaatatggct
     7381 tttcggctaa tgcgctatgc cactgccgcc atgcagcgtc acctggataa aggctatgac
     7441 agagttccgc tggtggtgcc attgctgttt tatcatggcg aaacatcgcc ctacccgtac
     7501 tcacttaact ggctggatga gtttgacgat ccgcaacttg cccggcagtt gtacaccgaa
     7561 gcttttccgt tggtggatat caccatcgta cctgacgatg agatcatgca acatcggcgt
     7621 atagctctgc ttgaactgat tcaaaagcat attcgcgacc gcgatttaat cggtatggtc
     7681 gacaggatca ccacgctttt ggttaaaggc ttcactaatg acagccagct acaaacactg
     7741 tttaattatc tgctgcaatg cggcgatacc tcccgtttca cccgttttat tgaggagatt
     7801 tccgaacgtt caccactaca aaaggagaga ttgatgacta ttgctgagcg gctacggcag
     7861 gaaggacacc aaattggctg gcagaaaggt atgcatgaac aagccattaa aattgctttg
     7921 cgcatgctgg aacagggcat tgatcgtgac caggtgctcg cggccaccca gctaagcgaa
     7981 gccgatctgg cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc
     8041 tttgatatca ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca
     8101 gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg
     8161 ctccccacgc caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt
     8221 tacccgcagc acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc
     8281 cggcgttatc cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat
     8341 caatcccttc gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct
     8401 gcactaacag acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc
     8461 gcgcagcccg tgccacactg gccccaacac cacgctcacc gtagggagga tagcgcgtgg
     8521 cagacgccac ctgacgagcc tgatcggcag tatcgaccat cgggatcaat agagtttgcg
     8581 cgccaatatc cagaacttgt ttaatcagcg atttactgcc ttccaccgga cggatcacgg
     8641 gttggctggc atagggcgct accgcctgta gctggtgata aagatcctga atggtgtttg
     8701 gcgcgtgctc cccgtcaatc agcaaccagt cataaccgga agtagcggca atttctgcca
     8761 tataagaagt agtcgagctt aaccacagac caatttgcac ttcgcccttg cgtaaacgtt
     8821 ctttaaaggg attggttaat aatgcgttca tcgtgttcct taacctattt aatgatgtgc
     8881 cacgtcggtc tgcgcaacag tacgatttac acgcagagtg aaaataatca gcgaaccgat
     8941 gaccgccacc gctgccagtg tcagtaatcc tgccgcatcg ctggcaaata acgtttctgc
     9001 tttcacgcgc aggatcgggg caataaaccc gcccactgca ccaaacaggt ttacaaaacc
     9061 aatccccgca gccagcgccg taccggaaag cagttgtgtc ggcatcgtcc agaacaccgg
     9121 ctgcacggca ataaacccga tagctgcaac acacagcgct acgatcgcca ttactggaga
     9181 aagcagcccg gacagaccaa taccaatgcc cgccgccagt aatgtcagcg ctgcgacatt
     9241 acgccgttcg ccggttttat cggaatagcg cggaataagc caggtcccaa acaaggccgc
     9301 aacccacgga atggcggtga ccaccgacgc tgtaaagccc acttttgttc ccagcaatgc
     9361 cgcaacctgg gtcggcagga agaaaattaa tccgtaaacc gccacctgaa tggtcaggta
     9421 gataatcgct agttgccaga ctcggccatt acgcagcgca tcgctcagcc gagaagtcac
     9481 tttctgttgt tcttcacttg ccagttgatt gataagcaac gttttttctt gtttactcag
     9541 aaaacgtgcc tgctccggtg tgtcatcaag ccaaaagaat gtgaataccc cagcaccgac
     9601 tgccaacaat ccttcaatca caaacatcca gaaccagccg ggatgcccca taaatccatg
     9661 catctccaac agcgcgccgg aaagcggtga tcccagtgtt aacgccagcg gtgcgcccat
     9721 atagaacagc cccataatgc tggcgcgatt acgctgcgga aaccattgcg aggtgagata
     9781 aatcatacca gggaaaaatc cggcctccgc agcaccaagc agagtgcgaa ctatcagaaa
     9841 tttcgcttca gtatcggccc atgccatggc tgccgaaaga aatccccaca gcagtgttgt
     9901 cgtaccaatc caggttctgg cccccagttt gcgcatcaaa agattcgccg gaacacccag
     9961 aaacgcatat accacaaaga aaatgcctgc tcccagcgca taagcttcat tactcaaccc
    10021 ggtatcaatc tggtaggtct gtttggcaaa accgatattc gaacggtcga gaaacgccag
    10081 cacatacagc gccaacataa acggaattaa acgcacacgg tttttcttca ccacggcgtc
    10141 aagcaaagcg gtgctcataa aaagctcctt agaatatgtg ggcgttaccg ttgtcacagc
    10201 aacgcccgga gtgattagtg gctgtagggg cgtttcaaat tgcagtcacg attgagttcg
    10261 acgccaaaac cgggtttatc aagcactgat ttatgaatac gaccattcac cggaaccggc
    10321 tcattgagca gaatcgggtc aaactgcgga cgcatcgttg aacaatccgg gctggtcatc
    10381 aggaattcgc tgaatggcgt attggtgaag gtgatcaccg catggtgaga gtaaacagac
    10441 gaaccgtgcg gcaccaccag ttgccccctg gatttggcga ttgcggcaat ttccaccagc
    10501 gtggttaaac caccgcacca gccaacatcc ggctgcataa tgtcgatacc ggtttctgaa
    10561 agcgtacgaa aagattgcag tgtgccgtgg tgctcaccgc tggtgaccat cattccgact
    10621 ggcgcgttgc gtttcagttc gcgataacct tcatactgct gtggcggcag gcactcttcg
    10681 atccatttca ggttataggg cgcgcaagcg tgggccagtt tggtggcata gttcacgtcc
    10741 tgactcatcc agcagtcgag cattaaccag aaatcctcac cgcatttttc acgcatatcc
    10801 gcgaccataa cggcatcttt gcggatcccc gcatcgccat catgtggccc ccagtgcgtc
    10861 ggcattttgc caccgataaa gcccatctct tttgccagat ccggacgcgc gcctgtggcg
    10921 tagaactgaa tctcatcacg aactgcgccg cctaaaagtt tataaaccgg aagcccgacc
    10981 actttgccga acagatccca cagagccaga tcgacacagg aaatcgtgtt catcaccagg
    11041 ccaccagagc cggagtaata cagggtggca ttgagcattt gatcatggat cagtttgata
    11101 tcactgacac atttaccctc aatgaaacgg ttaagatgtt tttcgacaat aaaacagccc
    11161 atttcaccgg ctgtcgaaac agcgaatccg gtctggccgt tttctgcttc gacttcaaca
    11221 atcaacgtgc caagaacgtt aatgccaaat gactggcgtg actgctcgta atcgcggtat
    11281 ttactcatcg gggtggcaat atgatcgtca atccagtgat tcgccccctg gtcgtgataa
    11341 tcaccgcgcc agcacctttt tctgctgtcg caccgccagt aaaccaggcg cgaacctgtt
    11401 tgatttttgg tagggtcatg atgttctcca ttgttatgag gcctgtaagt caaagggact
    11461 tttccatccc aacagacgtg aaatatccct ggcgcaggca atggccttgc ccgccagata
    11521 atcacggtat tcttcattga tttgtaagcg ggtaccgacc accgagatcg cagcggtaag
    11581 ctcgttattg gcgttaaaca ccggcgcagc gacacaacgg acatcggggt aatcttcgcc
    11641 gttgtcatag ctccagccct gacggcgaat acgcgccagt tcttcgtgaa gttgctgtgg
    11701 atgagtaatc gttgtgggtg tcgcctgctc ccagaccagc ccttcgataa tactttgctg
    11761 taccgctgca ggttgccagg caagcaggca tttacctatt ccggagcgat aaagcgaaag
    11821 gctttttcct tcatgggaac gcacgctgat agtggctgat gactccactt ttaaaatgta
    11881 ataagcgctg ccgttatcaa taatccccag gtgacataac agcccggtgg tatccatcag
    11941 ttgcgtcaaa cgcggtcgag ccagttcccg gagatccatc ttgcttaacg catgaccaga
    12001 aagctccacc agcctggtcc acaaacaaaa attttcctgg tgatcaaggc taagaaaacg
    12061 ctgacgcctg agttcattaa gcagcaaata ggccgtgctt ttagggattc ccaatgtgtc
    12121 aatgatcgtc gcagcactac aggggccaat gcgggcaatc aaattgagaa tatcaatagc
    12181 ccgtgtcagg gcgggaactt tgcttgattc caacatactg gactccagtc ttagatactg
    12241 gaatcagtct cgttgctcag ggggagtaaa attgtgaagc gcatcaaatc ccgctcctcg
    12301 tccagcggac tggagaaatt acgcacaaat ttgtgaatca gtgcacggat tttttcttat
    12361 tgagtacaac aggttggaca agttttgccg gagggggaat gtgccggtgc agtaaaccgg
    12421 cacagagtgg cggaatcaga cagataaggt catggactca acaacctcaa tccagccatg
    12481 ctcactggcg atatcctggc cgtttaacca acggcgcagc atattcagtg ccatcattgc
    12541 gcacacttcc tgacggatag ccaggctgta gcgtgtagtg ctgaaacgca cacgcagagc
    12601 gaaagtgccg tctggcgtgg ctagcgcaaa gttgagatgc tcgttctcga aacccgaaac
    12661 agccagtgcc agcccggcaa aatggttggc ccgccgttct gtaatccagt gcgcagtttg
    12721 cgccagggtt tcttcctgtg aaggaaccac ttcacacgcc agcaatggag cacctgcgcg
    12781 agaaagttgc aaagccaata aaccaccggt gaattgctcg ctcaacgtca ggctgaactg
    12841 gcggttttgc aattcgcgac tgatctgcgc gggcagtcct tcggtgcctt cgaaaatcac
    12901 gctctgtccg gcaacacgtt taacgtccag ccacagtttt tccatcgcct gttgctcgct
    12961 tgccggtccg gtgagtttca gttcgatgat cggcattgag gagcgatagc ccattgttac
    13021 gcccggcggc agttgtagag tgtccaggct ttgtgccaga tcgctttccg aacgaccaaa
    13081 ggtagttaaa cgcagacaaa ccggcgactg tggtaaagaa aaacgctcgc gcaggcgcgg
    13141 caggatttcg tgctcgacca tcaccttaaa ttctgacggt acgccgggag taaagaacat
    13201 caggcaacga ttaagccgca cggcaaaacc acaggcggtg cctaccgggt tattgataaa
    13261 ttcagcactg gcaggcagct ccgcttgttt acggttgctc ggtgccatta ctcgtccacg
    13321 ttcgtggaaa tagcgttcca tctctttgag ccaggcttca tgcagcacca ggccttcacc
    13381 ttttgctgtc gcagcggcga gtgcacttaa atcatcactg gtcggtccca gcccgccgtt
    13441 aacgatcagc acatcggcgt gctgactacg ttcgcgaaga atggtgacta agtcatcaag
    13501 gttatccccc accgtattgc ggcgagataa tggcaacccc tgatgaaaga aaaaatcggc
    13561 cagccaggca gcgttagtgt caacgatttg cccgtgtaac acttcatccc cggtggataa
    13621 catttccact tttaacatgg ttatctcctg cttttgttat cgaatcacta taacgcaaag
    13681 ggagataagg cgtagtaaga aactgaatgg cgcgacagaa tgccgcgccg gaagggatca
    13741 gaaactggcg ttaaccccaa catacgggcc gtcagccacg gcgttgtcgc ggttaccgtc
    13801 tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggat gcataatggt
    13861 gtaacgcgcg ccagcattcg cttcctcata acttttaata ccgctggaga gtgaatccgg
    13921 agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact gcaaaccacc
    13981 tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaaa cgccttttcc
    14041 gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca cgccagcgac
    14101 gtcaccgtcg tcgtcgttat gtgtccagtt accgcttaac gccaggcccg tcgattcagt
    14161 cccaaaaccg acaccaatat tggtgtattc tttacccgcc tgaccgctga tgctcattgc
    14221 attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa tttttttcat
    14281 tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt gtactgcctg
    14341 gcacagagga ttcaatgagc gaaaaaaggg atctagtcac ttattgtaac taaatacttc
    14401 ttaaataatt aaacgccgaa aaaataaaca cactttaccg atacacacaa aaataataat
    14461 aagtgcagtt atcatcgcaa aagagattac tgtcactttc acatatttct gagttaccgt
    14521 attctcatgg tttcttttta tttataagga atacccctgt gcgacaacgg actattgtat
    14581 gccctttgat tcaaaatgat ggtgcttatt tgctgtgcaa aatggccgac gatcgcggcg
    14641 ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag
    14701 aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg acagaaatca
    14761 cgccgtggac cttcagcgat gatattcgca ccaagacgta tgcagatggt cgcaaggaag
    14821 agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa gtgaaaataa
    14881 acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa
    14941 atgttgccac ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga tgacagccat
    15001 cactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga taaactttgc
    15061 ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatgtc gcatcacgct
    15121 tatttttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata tttttatcag
    15181 gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac tgaagaagtc
    15241 gtttatctac cgtcagtttt ttacccgcgg aaaaccaggt cgccgactga atggtccgga
    15301 cggtattact ggaataaaga tcgaaatcag ggatgtcagc gctaaaagcg ttgcccagtt
    15361 cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt atctgacaag cattgattgg
    15421 ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca
    15481 gtctggccaa tgttttattg tcgatacgtg gcaaaccatt gctactccag gcggcatgag
    15541 tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt tttgacttca
    15601 atgatgagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc aaccagacaa
    15661 ttaccgtgaa attgagcgac atttctggcg ataattagca gttggtgtaa tattaaaaat
    15721 cctacgatgt cggcaaaatg cctcaaaatt ttgccaaatg caaagcctaa ataagaaaaa
    15781 atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt taatattcta
    15841 tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
    15901 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
    15961 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca
    16021 caaccggtcc gaagaatcag gcgcttgagc aagccttttg ccagttgacg ggaaatcagc
    16081 atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
    16141 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
    16201 tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca
    16261 cgcctgaagc gatcgagtca gccattacgc cacgcactaa agccatcatt ccggtgcatt
    16321 atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg
    16381 cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg
    16441 caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt gcggaaggtg
    16501 gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg aaatttcacg
    16561 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag gctgaagtct
    16621 taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc ctgacacagt
    16681 tagcaaaatt agagcacctc aacacccatc ggcgcgaaat tgcccagcaa tatcagcaag
    16741 cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac gttcacgcct
    16801 ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga
    16861 tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac
    16921 aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa tggaatagcg
    16981 aacgcatctg ttctttgccg ctgttcccgg atatgactac cgccgatgcc gaccgtgtca
    17041 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
    17101 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
    17161 gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg attgatgacg
    17221 gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag aacagccata
    17281 ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca
    17341 gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat ccgccagaag
    17401 aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc
    17461 agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa gatgattaac aggcttattc
    17521 agcgcaccac cggcaaagcg atgggtgact acggttgtat gctgcgcgcc tatcgccgtc
    17581 atattgtcga tgcgatgttg cactgccatg aacgtagcac ctttatcccg attctggcga
    17641 atatcttcgc ccgccgtgcc attgaaattc cagtacatca tgccgagcgt gagtttggtg
    17701 aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
    17761 ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt ggaggtttta
    17821 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
    17881 gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
    17941 gattgctcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
    18001 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
    18061 tgcctaccac gatatgggat gcctcggtat tgaagctctg ctggctgccg gttacgaaat
    18121 tagcgccatt tttacccata ccgataatcc cgctgaaaaa gccttttatg gttcggtggc
    18181 tcgtctggcg gcggaaagag gcattccagt ttatgcgccg gataacgtta atcatccgct
    18241 gtgggtggaa cgcattgccc aactctcacc agaggtgatt ttctcttttt attatcgcca
    18301 tcttatttgc gacgagattt tgcagctcgc tcccgcaggt gcatttaatc tgcacggttc
    18361 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac
    18421 ggaaacgggc gttacattgc accgaatggt aaaacgtgcc gatgctgggg ccattgtggc
    18481 gcaactgcgc gttgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
    18541 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
    18601 ggaaattgcc cagcgcgaaa acgaagccac ctgttttggt cacagaacgc cagatgacag
    18661 tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg ccgttgccga
    18721 tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg
    18781 tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg cgccactgct
    18841 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
    18901 tatgcagggc tcgcaattag cgcagatgct gggcctggtg caaggttcac gcttgaatag
    18961 ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
    19021 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
    19081 ggatattggc agcgatgcga taagccgttt tctgaatcat ccgcattttc actttgtcga
    19141 aggcgatatc agtattcatt ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt
    19201 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt
    19261 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgttaagt accgtaagcg
    19321 aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat acttcgatga
    19381 ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtgtc
    19441 aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt tacagttcac
    19501 cctcttccgc ccgtttaact ggatgggacc acgactggat aaccttaatg cagcgcgaat
    19561 tggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccaattaa
    19621 gctaattgat ggcggaaaac aaaaacgctg ctttactgat atccgggacg gtatcgaggc
    19681 gttgtaccgc attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg
    19741 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
    19801 aaaacatccg ctgcgccatc acttcccgcc gtttgcgggc tttcgtgttg tcgaaagtag
    19861 cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
    19921 ccgccgctgc ctggactggg aaccgaaaat tgatatgcag gaaaccatcg acgaaacgct
    19981 ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtcggctta
    20041 cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccacgtct gctggaaatc
    20101 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
    20161 cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg
    20221 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt
    20281 ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt cggcctgcac
    20341 gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca aacaatgatc
    20401 gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc ggtaacctgt
    20461 tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga agcgttccat
    20521 ttgcgctaca acagcgattg tcgtggggcc atgccgttcc gtccactgct cgaatcagga
    20581 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
    20641 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
    20701 acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
    20761 gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactgtta
    20821 tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
    20881 ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac cttatcggcc
    20941 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctctggcaac
    21001 ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc gactggattg
    21061 tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
    21121 gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg
    21181 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
    21241 gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca
    21301 cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg tgcagcttct
    21361 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa
    21421 tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
    21481 gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt tacggctggc
    21541 tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg
    21601 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactggatg
    21661 atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc ggtagcctgc
    21721 cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc aagcattccg
    21781 caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag
    21841 gtaaattgcc cacctatatt ctttcctgct ttgcatctct ggcaatgctg atggcgcatt
    21901 acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
    21961 tcgcttttgg cttcactggc attattgcca catttgtggt ctccccgtgg ggaccaatga
    22021 acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
    22081 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggtcttttg
    22141 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca
    22201 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
    22261 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
    22321 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
    22381 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
    22441 aggggattat tacactcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca
    22501 ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag tatcgtccca
    22561 aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca
    22621 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
    22681 gggactggcg ctggcttgtc ttggtcttgc catggtgctc tggctgctgg tcttgcagaa
    22741 cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg tgacgctggc
    22801 tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat
    22861 tattggcggc attgtgatcc tcgggagtac ggtgtaatgg gcctgatgtg gggattattc
    22921 agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg
    22981 ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttggcct ggatgcccgg
    23041 atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacgcta
    23101 cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg
    23161 attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga
    23221 gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa acaacgttac
    23281 tgagttttcc ctgccacttt acactgccgt tcccactcat cggggctata actgcttgcc
    23341 gagaggactt tcaccgtatg cagcttttca cgattaatac aataaagcgc attctgcaaa
    23401 ggcgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc gatcattttt
    23461 atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt attgcagcac
    23521 gattttttga ccagccattc cattgcatta tcctgtttgc taagagtttt cacatcaatt
    23581 tacagattaa cagataaacc tattatcaac ggcaatggaa tggcaaaatt gcgaagtgaa
    23641 actaatgatt tcgtaatcct ggcgttgcac ccactctttt aacgcctgac gtgaaatttt
    23701 aataccgccg tttttcagct ccggcggtaa agttagccag cgcaccggtt gttgaaaacg
    23761 agccagctta tctttcaccc attcgccaag atcaacggtt tgctggtcat actccaccac
    23821 cgctaccggt cgatgaccaa actccttgtc ggcaacgggg acgataaaca cctgcaaaac
    23881 cgcaggatgt gcagcaatta cgcgctcgac ttcttccggc tgaataccct ctccgccact
    23941 gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg ccattatgca tctcaccgcg
    24001 atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa accagttgcc cgttacgcca
    24061 gtaaccttct gccatactgg cagcccgcag ccacacttca ttattaacga ttttcacttc
    24121 ccgacccggc agcggcgaac caacgtctgc caggccgtcg gcttctttcg cacacaccgt
    24181 ggaggcaaac tcggtcagac catagccgca aaagcaacga atcccctgct cgcgcgcctg
    24241 ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac accgctttca gggaaacgga
    24301 actgcggtta accagcaaac gccagagttg tgttggcacc agtgaagcgt gagtacagcc
    24361 tgccagcatt tgctccaatg gctgtttatc acgcaccgtc atccgcgcac cagcgtataa
    24421 ccagcgccac ataattccct gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc
    24481 atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc gcactggcaa gatgggcctg
    24541 ataagtatgg acagcggctt tcggcaaacc ggtagaaccc gaggtcaacg tcattgagca
    24601 cagacgcgtc ggctgccacg tagcggcatg tgcgccttca acccgctgaa tgtgcagcga
    24661 cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc gcaaattgca gcgtcagatt
    24721 gggcagcaat tcttcaagca acggttgcgg cagctgaggg ttcacgggca acacccgcgc
    24781 cccgcattgc agtaacgcca gccaggcgag cagcgtttgc ggcgtattcc acgcccgcaa
    24841 catcacgccg ctgccctcaa ccacaccctg cgccgcaaag cccgtagcta actcatcgac
    24901 gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta agacgtaagg cgatggcttc
    24961 tccccgcact tgccgccagt gacgccacgg ccagtcagag aagatcataa caactgctcc
    25021 agtacatcag cgtccactaa cggcagtggg ctccccggcc agcgacgaac ctgctgcgcc
    25081 tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg gcgttaacca ggcagcaatc
    25141 cgcgccagtt gcgttagacc taagctcgac tcaatggaag aactgatcac cgccgtcagc
    25201 cccagcgcat gcgccgcctg tacctgctcg cgtacttttt ccagactgcc cgtgagcgtg
    25261 ggtttgataa ctaccgcgcg cacgccctct tcagccacaa aggcaaaatc cggctcgcgc
    25321 aggctttcat cccaggcaat ggcaatgccg gtttcacggg caaacgctcg cgaatcatcg
    25381 cgggttttgc acggctcttc gagaaacgcg atgcggtggc gataatccgg gttaacatat
    25441 ttggcaaact gctgaccttt cagcggtgtc caggcgcgat ttgcgtcaag acgcaaatgc
    25501 agatccggaa ttgcctccag caacagatta accaccatgc cgtcgcgcac ggcttcgtac
    25561 aatcccactt tgaccttcgc cactttctcg cctggcatat cagcaagttt gaggatcaga
    25621 tcgtcaggat cgccattaca cagtggtgcc gcacggtaat tggccgcctg cgggagcgta
    25681 tctgccatct ccgccaatgc acagcttacg ccaaaggcaa cggaaggcat ctgcggtagc
    25741 tcgccatcgc ctgccagcca gttatttacc caagcaagca gcacaatttg cgcctcttcc
    25801 caggtttcct gactgaagcc cggcagtggg gagatctccc cccacccttc gcgctcgcct
    25861 tcacgcaggc aaacatacag cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccatc
    25921 cccgcgtcca tggggatctg ccagcggtat acctgcgcgc tacgcattac ggattccgtt
    25981 taaatttgct gaaatcaggc tgacgtttct ggttgaaagc gttgcgacct tcctgacctt
    26041 cttccgtcat gtagaacagc atggtggcgt tgcccgccag ctcctgcaga cccgcctgac
    26101 cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag cgccatcggg ctgttttgca
    26161 gcatttcgcg gcaccaacgg acggtttctt tttccagatc cgccagcggt accacggtgt
    26221 tgacaaggcc catatccagc gcctgttttg catcgtactg acggcacagg aaccagattt
    26281 cacgcgcttt tttctgtccg acgatgcgag ccatgtagga agcgccccag ccgccgtcga
    26341 aggaaccgac tttcgggcca gtctgaccga agatggcatt atctgccgcg atagtcaggt
    26401 cgcacatcat gtgcagaacg tgaccaccgc cgatggagta gccagccacc atcgcgacaa
    26461 ccggtttcgg acaggtacgg atctgacgct ggaagtccag cacgttcagg tgatgtacgc
    26521 cggaatcatc tttatagccg ccgtaatcac cacgcacttt ctggtcacca ccggagcaga
    26581 acgctttatc gcctgcgcca gtcagaatga tcacgccgac gttgtcgtca taacgcgcat
    26641 ctgccagtgc ctggatcatc tcttttaccg tcagaggacg gaaggcattg cgcacctgcg
    26701 gacgattaat ggtgattttt gcgataccgt cggtggattt ttcataacga atgtcctcaa
    26761 aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag cattgcttca tcaggataaa
    26821 tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc agacttgcga taacgccagc
    26881 gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg acatggcagt cggcagccag
    26941 ttccgccgcc aaggcgcgga atttgctgtc acgttcacca cataaataat aaaacgcaaa
    27001 tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg gcgagagaag tcgcctccag
    27061 catggcggca agggttgcgc cattattgtt actgcgcagc gccaccagct cccggcgttg
    27121 atcgtcattg agcgaggcaa aaacaggctg ttgataccag tcggcaaata ccgccgttaa
    27181 cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa cgctgacgtt ccgcgcgttg
    27241 ttcagcattt tgcaacccag gatgcccgcc ttcgacaaca accccacaaa gccccggcat
    27301 cccctggcaa gccgccatca tcgccacccg tcccccaagc gagtacccca ccaaccagaa
    27361 gttaaggatg ttgtaactaa ccagggtttt acgcagtaag tcggtgacat catcaaatcc
    27421 atcgacgcta atcgccgccg aaccaccgtg acctgggaga taaacataca accgtgagta
    27481 gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa tcgccggaaa aaccgtgcaa
    27541 aaacaccagc cagggtaaac ctggtttgcc gtgttttgcc tgcgcgtgca ggatcataaa
    27601 tggcttacct gcgccagaag ttgctggagc gtttgcgcac catcggtgtc gttaaccacc
    27661 atttcaatta ccgtggtggt tggcgtgcgc caggcatcgg caagtgccgt ttcaagttcc
    27721 tgccagtttt gcggacgatg atatttcagc tcgaacatcg cggcggcgtg ctcaaaatgg
    27781 acgttttgcg gcatcagata gaaacgctcg cgctcgcttt tcggcgttgg cagcagcgag
    27841 aaaatttgcc cgccgttgtt gttcaccaca attaatatca gcggcgcgga aacctgacgc
    27901 aataacgcca gcgcgttgag atcgtaaagt gcggagagat cgcccacaat cgccagcgtc
    27961 ggtttgccgc ttgcccgctg aacgccggcg gcagtcgaga gcagcccgtc gataccgctg
    28021 gccccacggt tgctgtacac cgggtaacct gccggaagtt gcgaaagcgc atcaatcaga
    28081 cgtaccacca ggctgttacc gacaaacaat tgcccctgtt caggcagata gtcgctgatg
    28141 cgatgcgcca gttgcgcttc gccaaacgca tcgcgacggg caataaccgc ctgcattgcc
    28201 tgttccgcca ggcgcgggat ttcaacgcac cagggctggc gtttttctgc cggatgcagc
    28261 tccagccagt cggcaatatt ggcaattaag cggcgtccac gatggtgcgc cggatcaagc
    28321 cgcccttcga tgtcatccac aatccagtac tcttcgggtt cacagcttgc ctgccattgc
    28381 aggagccgtt tgcccgtcag gctgcttccc agttgcacca caatttgcgc ctgttgcagt
    28441 tcgctggtcg ctttggcatt gcctaaccag agatcggcac acggcagcgg ctgtccggtt
    28501 tgcgacagca cgtcgccaat cagcggccag ccaagagttt gcgcccacag tgcaactttt
    28561 ttgccctctt ccgcactcat gcgcccggca accaccacgc cgcgtttttg tcgccagaag
    28621 aaccagtcgc gctgtttttc actttccaga cgaggcgctt cacgcagcca cggtttgtcg
    28681 tcctgccacc agtcgcccag ccgctgttgc cagctaagcc cggtgtcgtc catttcgcca
    28741 tacagcggtt cggcaaacgg gcagttgata tggactccac cagaatgaag cgtaccgaga
    28801 gcatggtcga tggtagaaac cagccaacgt gcggggatat cctgggtcgg gcgcggcaat
    28861 gaaatactgt gcgtggggtg agaggcgaac attcccggct gacgaatcgc ctgattcgcg
    28921 ccgcagtcaa tcagctccgg cgggcgatcg gcggttaaca ggatcagttt ttctccggtt
    28981 aacccagctt caatcagtgc cggatagaga tttgctaccg ccgtaccgga ggtcacaatc
    29041 accgccaccg gctgcttgct gactttcgcc agccccagcg ccagatgccc caacccacgc
    29101 tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg ccgccgctaa cgttaacggt
    29161 gtagaacgcg agccaggggc gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc
    29221 agaatgaccg ccgcccagcg tcggttaaat gcgcttactg acatgagttt gtccggtatc
    29281 aatattgcgg ctaagtataa ggagctacaa aaatagatta ttgatatgaa tcggtaatga
    29341 tgcgactcat tactattcca tctgtaataa agtacgcagc cctgccgctt tgttgtcgat
    29401 ttcctgccac tcttgctcgg ggtcggaacc acggacaatg cccgcgccag catataatcg
    29461 cacgacattg ccgctaattt ttgctgagcg cagggaaacg cagaattcgc tttgctgtaa
    29521 tgagagatag cccgcagaac cggcgtacca ttctcgggtg aacggttcgt gacgggcgat
    29581 aaactgtcgc gccagatcgc gcggtaagcc agcaactgct gccgttggct gcaactgatg
    29641 taaacagatc acattatccg ctttgttgag tgaagtccag atacagcggc gaagatgctg
    29701 cactttacgc agacgcagta cctgcggcgg taaaacatcc agcgtctggg tatcggcctg
    29761 taaccgttgg cagatatctt ccaccaccag catattctcg cgctggtttt tatcatccgc
    29821 catcagccac tcacctaact gctgcgcctg cttatcatca ggatgatttg ctaccgttcc
    29881 cgccagcgct tcagtacgca gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga
    29941 gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa tggtagcaat tcagattcag
    30001 tcgacgactg gcagccatca tcgccgccgc gttgaccgga cttgcgaaat gcaggtcagt
    30061 tgcccgagcg agcaccactt tgtcgagctc accttcggcg atggttttcg ttgccagttc
    30121 gattaattgc gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtgg tggttaaatg
    30181 taacccaggc aagggcttga tactcaccag tgtggcgata aattctcttg ccttaatcgc
    30241 atcgtgctga agggagcttt cgctgaatag cgtcagccgc agcgtggctt taccgccaca
    30301 gcgtcgccat tccaggcgtg gtaaaagtaa attgccctgc gacgggtcaa acgcattcag
    30361 cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc
    30421 caacgacgta aaacgggtaa tcgcgcccag gacggcagct tcttcatcac cattacgttg
    30481 ttgccagtag aattgcgggt atgtttgctg actggccagc cagctcaagg catcaaaagc
    30541 gtctttgaga gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg
    30601 cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt gattgcacgc ggacctctcc
    30661 ctgttaaaag cctcacatta tacggggtac tacaaaaaaa atgcagtacc ccggtgtagg
    30721 gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc ccaacggccg cgcccacacc
    30781 aattccttgc cagggttttt cgtggacgta gtcatcagca cgataaactg cctgcttcgc
    30841 ccgatagtaa taactgtctg aagcctggct aacccgtttt ttcacatcat ccagcgcttt
    30901 ttctgcacgc gctttcagct caacatattt ctgatcggcg ggatcgccag aggagcggag
    30961 cacctcttcc agtgtttcac taagcagcgt caggtcgtca tcgatacgtg tatcaccaaa
    31021 ctgattagac attctcgttc tccatttgcg taaaacctgt cactaactat agacaagggt
    31081 tgttgattac gcctgaatta cctcgcgtgc catcccaatg tgtgggatac catcttcttc
    31141 atagacctcc gtcacaggga taaaaccgaa actctggtag aagttttgca gatgcgcctg
    31201 cgccccgagg taaacaggct tatcaggcca gtgatgcgtg caggtgtcga gggttttact
    31261 catcagttgc tgccccactt tttcgccacg caacgcttca ctgacaatca cccgaccaat
    31321 aacgaccggc tcaagatcat catcactttt cagaatcctc gcatatgcca ccagttcatc
    31381 atttttccaa ccgagaatat ggcgattatc ccccgtcaaa tcatcaccat cgatatcctg
    31441 ataagggcaa ttctgctcga caacaaacac cgcgcaacgc agttgtaata aggcataaag
    31501 ctgggaaacc gataattcag agtgatgcag atcttaccat tcaatcataa tgctctcctt
    31561 ttagatgtat gccttatact agccggaata ttttttgaaa cgtgatgaac tcatggaatt
    31621 aattttttta ggtacttcag ccggtgtgcc aacccgcacg cgcaatgtca cggcaatatt
    31681 gcttaacctg caacatccga cccagagcgg actttggttg tttgactgcg gtgaaggcac
    31741 ccagcatcag ctactgcata ccgcctttaa ccctgggaaa ctggacaaga tttttatcag
    31801 tcaccttcat ggcgatcatc tctttggttt acccggcttg ctgtgcagtc gttctatgtc
    31861 aggcattatc caacccttaa cgatttatgg tccgcaaggt atccgtgaat ttgtggaaac
    31921 cgcgctgcgg attagcggct catggaccga ttatccgctg gaaattgtcg aaattggcgc
    31981 tggcgaaatt ctcgatgatg gcctgcgcaa agtgaccgct tatccgatgg aacacccact
    32041 ggaatgttat ggctatcgta ttgaagaaca tgataaaccg ggtgcattaa atgcccaggc
    32101 attaaaagct gctggcgtgc cgcctggccc actgtttcag gaattaaaag cgggcaaaac
    32161 aatcacgctg gaagatggaa ggcagattaa cggcgcagat tacttagctg ctccagtgcc
    32221 aggtaaagcg ctcgctattt tcggcgatac cggcccctgc gatgccgcac ttgacctggc
    32281 taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat ataaccatgg aagccaaagc
    32341 caatagtcgc ggccatagct ctacacgcca ggctgcgaca ctagcccgtg aggctggagt
    32401 cggcaagcta atcattaccc acgtcagctc gcgctatgat gacaaaggtt gtcaacacct
    32461 gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg gcgaatgatt tcaccgtgtt
    32521 taacgtttaa ctc
//
LOCUS       DAZPJR010000033        31627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPJR010000033 DAZPJR010000000
VERSION     DAZPJR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.034"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            156..491
                     /gene="eutS"
                     /locus_tag="ACHVB7_001408"
     CDS             156..491
                     /gene="eutS"
                     /locus_tag="ACHVB7_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR1913434.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            504..983
                     /gene="eutP"
                     /locus_tag="ACHVB7_001409"
     CDS             504..983
                     /gene="eutP"
                     /locus_tag="ACHVB7_001409"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR1913435.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            958..1659
                     /gene="eutQ"
                     /locus_tag="ACHVB7_001410"
     CDS             958..1659
                     /gene="eutQ"
                     /locus_tag="ACHVB7_001410"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR1913436.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1656..2459
                     /gene="eutT"
                     /locus_tag="ACHVB7_001411"
     CDS             1656..2459
                     /gene="eutT"
                     /locus_tag="ACHVB7_001411"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR1913437.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            2456..3472
                     /gene="pta"
                     /locus_tag="ACHVB7_001412"
     CDS             2456..3472
                     /gene="pta"
                     /locus_tag="ACHVB7_001412"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1913438.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3511..3804
                     /gene="eutM"
                     /locus_tag="ACHVB7_001413"
     CDS             3511..3804
                     /gene="eutM"
                     /locus_tag="ACHVB7_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR1913439.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3911..4198
                     /gene="eutN"
                     /locus_tag="ACHVB7_001414"
     CDS             3911..4198
                     /gene="eutN"
                     /locus_tag="ACHVB7_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR1913440.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4210..5613
                     /locus_tag="ACHVB7_001415"
     CDS             4210..5613
                     /locus_tag="ACHVB7_001415"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR1913441.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            5624..6460
                     /gene="eutJ"
                     /locus_tag="ACHVB7_001416"
     CDS             5624..6460
                     /gene="eutJ"
                     /locus_tag="ACHVB7_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR1913442.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6450..7637
                     /gene="eutG"
                     /locus_tag="ACHVB7_001417"
     CDS             6450..7637
                     /gene="eutG"
                     /locus_tag="ACHVB7_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR1913443.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7954..9180
                     /gene="eutH"
                     /locus_tag="ACHVB7_001418"
     CDS             7954..9180
                     /gene="eutH"
                     /locus_tag="ACHVB7_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR1913444.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            9177..10580
                     /gene="eutA"
                     /locus_tag="ACHVB7_001419"
     CDS             9177..10580
                     /gene="eutA"
                     /locus_tag="ACHVB7_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR1913445.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10592..11953
                     /gene="eutB"
                     /locus_tag="ACHVB7_001420"
                     /pseudo
     CDS             10592..11953
                     /gene="eutB"
                     /locus_tag="ACHVB7_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            11974..12861
                     /gene="eutC"
                     /locus_tag="ACHVB7_001421"
     CDS             11974..12861
                     /gene="eutC"
                     /locus_tag="ACHVB7_001421"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR1913446.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12871..13530
                     /gene="eutL"
                     /locus_tag="ACHVB7_001422"
     CDS             12871..13530
                     /gene="eutL"
                     /locus_tag="ACHVB7_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR1913447.1"
                     /translation="MPALDLIRPSVTAMRVIASVNSEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13543..14043
                     /gene="eutK"
                     /locus_tag="ACHVB7_001423"
     CDS             13543..14043
                     /gene="eutK"
                     /locus_tag="ACHVB7_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR1913448.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            14089..15141
                     /gene="eutR"
                     /locus_tag="ACHVB7_001424"
     CDS             14089..15141
                     /gene="eutR"
                     /locus_tag="ACHVB7_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR1913449.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(15147..16046)
                     /gene="hemF"
                     /locus_tag="ACHVB7_001425"
     CDS             complement(15147..16046)
                     /gene="hemF"
                     /locus_tag="ACHVB7_001425"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR1913450.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16050..16919)
                     /gene="amiA"
                     /locus_tag="ACHVB7_001426"
     CDS             complement(16050..16919)
                     /gene="amiA"
                     /locus_tag="ACHVB7_001426"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR1913451.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTVAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            17133..17558
                     /locus_tag="ACHVB7_001427"
     CDS             17133..17558
                     /locus_tag="ACHVB7_001427"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR1913452.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17545..17994
                     /locus_tag="ACHVB7_001428"
     CDS             17545..17994
                     /locus_tag="ACHVB7_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR1913453.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            18055..18630
                     /locus_tag="ACHVB7_001429"
     CDS             18055..18630
                     /locus_tag="ACHVB7_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR1913454.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18726..19625
                     /gene="yfeX"
                     /locus_tag="ACHVB7_001430"
     CDS             18726..19625
                     /gene="yfeX"
                     /locus_tag="ACHVB7_001430"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR1913455.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19683..20987)
                     /gene="pbp4b"
                     /locus_tag="ACHVB7_001431"
     CDS             complement(19683..20987)
                     /gene="pbp4b"
                     /locus_tag="ACHVB7_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR1913456.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20992..22416)
                     /gene="murP"
                     /locus_tag="ACHVB7_001432"
                     /pseudo
     CDS             complement(20992..22416)
                     /gene="murP"
                     /locus_tag="ACHVB7_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(22420..23316)
                     /gene="murQ"
                     /locus_tag="ACHVB7_001433"
     CDS             complement(22420..23316)
                     /gene="murQ"
                     /locus_tag="ACHVB7_001433"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR1913457.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23480..24337
                     /gene="murR"
                     /locus_tag="ACHVB7_001434"
     CDS             23480..24337
                     /gene="murR"
                     /locus_tag="ACHVB7_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR1913458.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24466..25257
                     /gene="ucpA"
                     /locus_tag="ACHVB7_001435"
     CDS             24466..25257
                     /gene="ucpA"
                     /locus_tag="ACHVB7_001435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR1913459.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25428..26444
                     /gene="cysP"
                     /locus_tag="ACHVB7_001436"
     CDS             25428..26444
                     /gene="cysP"
                     /locus_tag="ACHVB7_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR1913460.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26444..27277
                     /gene="cysT"
                     /locus_tag="ACHVB7_001437"
     CDS             26444..27277
                     /gene="cysT"
                     /locus_tag="ACHVB7_001437"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR1913461.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27277..28152
                     /gene="cysW"
                     /locus_tag="ACHVB7_001438"
     CDS             27277..28152
                     /gene="cysW"
                     /locus_tag="ACHVB7_001438"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR1913462.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            28142..29239
                     /gene="cysA"
                     /locus_tag="ACHVB7_001439"
     CDS             28142..29239
                     /gene="cysA"
                     /locus_tag="ACHVB7_001439"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR1913463.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29373..30284
                     /gene="cysM"
                     /locus_tag="ACHVB7_001440"
     CDS             29373..30284
                     /gene="cysM"
                     /locus_tag="ACHVB7_001440"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR1913464.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30287..30655)
                     /locus_tag="ACHVB7_001441"
     CDS             complement(30287..30655)
                     /locus_tag="ACHVB7_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR1913465.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30760..31611
                     /gene="pdxK"
                     /locus_tag="ACHVB7_001442"
                     /gene_synonym="thiJ"
     CDS             30760..31611
                     /gene="pdxK"
                     /locus_tag="ACHVB7_001442"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR1913466.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         7161 a         8172 c         9148 g         7146 t
ORIGIN      
        1 attcatgcgt tgcatatgaa agtttatgca ccacagcgaa tatctctcca ttcttagtga
       61 tctacctcac cttttaaacg cgcttgccga attttgttat ttactctgac gaaaaattgt
      121 cacgatacac gaaagttttt cacaggcggc gactcatgga taaagaacgc atcattcagg
      181 aatttgtgcc cggcaaacag gtcacactgg cgcatctcat cgcgcatccc ggtgaggaac
      241 tggcgaagaa aatcggcgtt ccggatgcgg gcgtaattgg cattatgacg ctaactcccg
      301 gcgaaacggc gatgatcgtc ggtgatttag cgctgaaagc cgctgatgtg catatcggtt
      361 ttctcgaccg cttcagcggt gcattggtga tttatggctc ggtgggcgca gtagaagaag
      421 cgttatcaca aaccgtcagc ggtttaggtc gcttattaaa ttatacgctt tgtgaaatga
      481 ccaaaagtta atacgaggca ttcatgaaac gaattgcgtt tgtgggtagc gtcggggcag
      541 gaaaaacaac gttatttaat gcgttgcagg ggaattatac cctcgccaga aaaacacagg
      601 ccgtggaatt taatgataag ggcgatattg atacgccggg tgaatatttt agccatcccc
      661 gctggtatca cgccttaatt accacgctac aggatgtaga catgctgatc tatgtccatg
      721 gcgcaaatga cccggaaagt cggttgcctg ccgggttgct ggatattggc gtcagcaaac
      781 ggcaaatcgc cgtcatcagt aaaacggaca tgccagatgc tgacgtcgcc gcaacgcgaa
      841 agttattgct tgagacgggt ttcgaagagc cgattttcga gctaaacagc cacgacccgc
      901 aaagcgtgca gcaactggtg gattatctgg catctctcac caaacaggag gaagcaggtg
      961 aaaaaactca tcacagcgaa tgatattcgt gaagcacacg cgcgcggcga acaggcaatg
     1021 tccgtggttc tgcgcgccag cattattacc ccggaagccc gtaaagtggc ggatctactg
     1081 ggttttacca tcaccgaatg tgatgaatcg atcccggtaa cggcgtccgt gcccgccagc
     1141 gtgccagccg acaaaaccga aagccagcgc atccgcgaaa ccatcattgc ccagctgccg
     1201 gaaggccagt ttaccgaaag cctggtcgca cagctgatgg aaaaagtgat gaaggaaaaa
     1261 cagtcgctgg agcagggcgc aatgcagccg agcttcaaat cggtgaccgg caaaggcggc
     1321 atcaaagtga ttgatggcag cagcgtcaag tttggtcgtt ttgatggcgc acaaccgcat
     1381 tgcgtgggct tgactgatct tgtgaccgga gacgacggca gcagcatggc cgccggtttt
     1441 atgcagtggg agaacgcatt cttcccgtgg accctgaact acgacgaaat cgacatggtg
     1501 ctggaagggg agctccatgt tcgccacgaa ggcgaaacca tgatcgccaa agcaggcgac
     1561 gtgatgttta tcccgaaagg ctccagcatc gaatttggta cgacatccag cgtgaaattc
     1621 ctgtatgtcg cctggccggc taactggcaa tccctatgaa agatttcatc accgaagcat
     1681 ggctaagagc gaaccatacg ctcagcgaag gcgcagagat ccatctccct gcggacagtc
     1741 gcctgacgcc ctctgcccgg gagttgctgg aaagccgcca tctgcgcatc aagtttattg
     1801 acgagcaggg ccgcctgttt gttgacgatg aacagcagca gccgcagccc gttcatgggc
     1861 tgaccagtag cgatgaacat ccgcaggcgt gctgcgaact gtgccgccag ccggtggcaa
     1921 aaaagccgga tacgctgact cacctgtcgg cggaaaaaat ggtcgccaaa agcgatccgc
     1981 gtctgggttt tcgcgcggtt ctcgacagca ccattgcgct ggcagtgtgg ctgcaaattg
     2041 aactggcgga accgtggcag ccgtggctgg cggatatccg ttcgcgtctt ggcaacatta
     2101 tgcgcgccga tgcgctgggg gaaccgctgg gctgccaggc gattgtcggg ctttctgacg
     2161 aagatctgca tcgactttct caccagccgc tgcgctatct cgaccacgat catctggtgc
     2221 cggaagccag ccacgggcgc gatgcggcgt tgctcaatct gctacgtacc aaagtgcggg
     2281 aaaccgaaac cgtagcggcg caggtgttta tcacccgcag ctttgaagta ttacgcccgg
     2341 acatcttgca ggcgctgaac cgcctttcga gcacggtcta cgtaatgatg atcctgagcg
     2401 taacgaagcc gccgctaacg gtgaagcaga tccaacaacg actgggggaa acgcaatgat
     2461 tattgaacgt tgtcgtgaac tggcgttgcg agcgcccgcc agagtggttt ttccggatgc
     2521 gttagatcaa cgtgtgctga aagctgcgca atatttacat caacaaggtc tggcaacgcc
     2581 cattctggtc gctaatccgt ttgaacttcg tcagtttgcg ctcagtcacg gcgtggcgat
     2641 ggacgggcta caggtgattg atccgcatgg caacctcgca atgcgggaag aatttgctca
     2701 tcgctggctg gcccgcgcgg gcgaaaaaac gccgccggat gcgctggaaa aacttaccga
     2761 tccgctgatg ttcgccgcag caatggtcag cgccggtaaa gcggatgtct gtatcgcggg
     2821 caacctctct tccacggcga atgtgctgcg tgccggatta cgtattatcg gcttgcagcc
     2881 aggctgtaaa acgctctcat ccattttcct gatgctgcca cagtacagcg gtccggcgtt
     2941 gggctttgcc gattgcagcg tggtgccaca gccgacggcg gcgcaactgg cggatatcgc
     3001 gcttgccagt gccgaaacct ggcgcgccct caccggagaa gagccgcgcg tggcgatgct
     3061 gtcgttttcc agtaacggta gcgcccgtca cccctgcgtt gccaacgtcc agcaggcgac
     3121 agaaatcgtc cgtgagcgcg caccaaagct ggtggttgat ggcgagttgc agtttgacgc
     3181 cgccttcgtg ccggaagtgg cggcgcaaaa agcgcctgcc agcccgctac agggcaaagc
     3241 caatgtgatg gtttttccgt cgctggaagc cggaaatatt ggctacaaaa tcgcacaacg
     3301 actcggcgga tatcgtgccg tcgggccatt gatacaagga cttgccgcgc cgatgcacga
     3361 tctctctcgt ggttgtagcg tgcaggaaat tatcgagctg gcgctggtgg cagctgtgcc
     3421 gcgtcagaca gaagtgaacc gcgaaagcag tttacaaaca ctggttgaat gaacggtccc
     3481 gttctggacc ctgataaaga ggaaagcaca atggaagcat taggaatgat cgaaacccgg
     3541 ggcctggttg cactgattga ggcttctgac gcgatggtta aagctgcgcg cgtcaagctg
     3601 gttggtgtta agcagattgg cggcggcctg tgtactgcca tggttcgtgg tgatgttgca
     3661 gcctgtaaag ccgcgaccga tgcaggtgcc gcagcggcac agcgtatcgg cgagctggta
     3721 tctgtacacg ttatcccgcg tccgcacggc gacctggaag aagtcttccc gatcggcctg
     3781 aaaggcgata gcagcaacct gtaatcaccc tgtgtgacgt cgattcgggc gtcacgctct
     3841 ctcctttttc attttacctt ctgcggaggg tagggggagc ttttacctga aatacgccac
     3901 ggaggcgggt atgaaactgg cagtcgtcac tggacaaatt gtttgtaccg tacgccatca
     3961 cggactggcg catgacaaat tgctgatggt ggaaatgatt gatccacaag gtaatcctga
     4021 cgggcaatgc gccgtcgcca tcgacaatat tggcgcggga accggggagt gggtgttact
     4081 ggtgagtggc agttccgccc gccaggcgca taaaagcgaa acgtcaccgg tcgatctgtg
     4141 cgtgattggc attgtcgatg aggtggtgtc tggcggtcag gtaattttcc acaaataagg
     4201 cagaacatca tgaatcaaca ggatattgaa caggtggtga aagcggtact gctgaaaatg
     4261 caaagcagtg acacgccgcc cgccgccgtt catgagatgg gcgttttcgc gtccctggat
     4321 gacgccgttg cggcagccaa agtcgcccag caagggttaa aaagcgtggc aatgcgccag
     4381 ttagccattg ctgccattcg tgaggcaggc gaaaaacacg ccagagattt agcggaactt
     4441 gccgtcagtg aaaccggcat gggccgcgtt gaagataaat ttgccaaaaa cgtcgctcag
     4501 gcgcgcggca cgccaggcgt tgagtgtctc tctccgcagg tgctgaccgg cgacaacggc
     4561 ctgactttaa ttgaaaacgc gccctggggc gtggtggctt cggtaactcc gtccactaac
     4621 ccggcggcaa ccgtaattaa taacgccatc agcctgattg ccgcgggcaa cagcgtcatt
     4681 tttgccccgc atccggcggc gaaaaaagtc tcccagcggg cgatcacgct gctcaaccag
     4741 gcgattgttg ccgcaggcgg gccggaaaac ttactggtta ctgtggcaaa tccggatatc
     4801 gaaaccgcgc agcgtttgtt caagtttccg ggcatcggcc ttctggtggt gaccggcggc
     4861 gaagcggtag tagaagcggc gcgtaaacac accaataaac gtctgattgc cgcaggcgct
     4921 ggcaacccgc cggtagtggt ggatgaaacc gccgacctcg cccgtgccgc tcagtccatc
     4981 gtcaaaggcg cttctttcga taacaacatc atttgtgccg acgaaaaggt actgattgtt
     5041 gttgatagcg tagccgatga actgatgcgt ctgatggaag gccagcacgc ggtgaaactg
     5101 accgcagaac aggcgcagca gctgcaaccg gtgttgctga aaaatatcga cgagcgcgga
     5161 aaaggcaccg tcagccgtga ctgggttggt cgcgatgcgg cgaaaatcgc ggcggcaatc
     5221 ggcctgaacg tgccgcaaga aacgcgtttg ctgtttgtgg aaaccactgc agaacatccg
     5281 tttgccgtga ccgaactgat gatgccggtg ctgcccgtcg tgcgcgtcgc caacgtggcg
     5341 gatgccattg cgctggcggt gaaactggaa ggcggttgcc accacacggc ggcaatgcac
     5401 tcgcgcaaca tcgaaaacat gaaccagatg gcgaatgcta ttgataccag cattttcgtt
     5461 aagaacggac cgcgcattgc cgggctgggg ctgggcgggg aaggctggac caccatgacc
     5521 atcaccacgc caaccggtga aggggtaact agcgtgcgta cgtttgtccg tctccgtcgc
     5581 tgtgtattag tcgatgcgtt tcgcattgtt taaggagtaa cagatggcgc acgacgaaca
     5641 atggctcacc ccacgtctgc aaacggcggc aacgctgtgt aaccagacgc ccgccgcgac
     5701 ggaatctccg ctgtggctgg gggtggatct gggcacctgc gatgtggtgt cgatggttgt
     5761 cgaccgcgac ggtcagtcgg tggcggtatg cctcgactgg gccgatgtgg tccgcgacgg
     5821 catcgtctgg gatttcttcg gcgctgtcac cattgttcgt cgccatctcg acacgctcga
     5881 gcagcaattc ggtcgccgtt ttagccatgc ggcgacttca tttcctccag gtacggaccc
     5941 gcgtatttcc atcaacgtgc tggagtctgc tggactggaa gtgagccatg tgctggatga
     6001 gccaaccgca gttgcggatc tgctgcaact ggacaacgcc ggtgtagtgg atatcggcgg
     6061 cggcactacc ggcattgcca tcgtgaaaaa gggcaaggtg acgtactcgg cggatgaagc
     6121 aaccggcgga catcacatct ctctgactct cgccggaaat cgccgtattt cgctggaaga
     6181 ggcggagcag tacaagcgcg gtcacggtga agagatttgg cctacggtga aaccggtgta
     6241 cgaaaaaatg gcggacattg tcgcccggca tattgaaggg cagggcatta ctgatttatg
     6301 gctggcgggc ggctcttgta tgcaaccggg cgtggcggaa ctgtttcgca aacaattccc
     6361 ggcgttacag gtgcatttac cgcagcacag tttgtttatg accccgctgg cgatcgccag
     6421 tagtgggcga gagaaagcgg aggggctata tgcaaaatga attgcagacc gcgctctttc
     6481 aggcgttcga taccctgaat ctgcaacggg taaaaacatt tagcgttcca ccggtgacgc
     6541 tttgcggtcc gggctcggtg agcagttgcg gacagcaagc gcaaacgcgt gggctgaaac
     6601 atctgttcgt gatggcagac agctttttgc atcaggcagg gatgaccgcc gggctgacgc
     6661 gcagcctggc tgttaaaggc atcgccatga cgctctggcc atgtccggtg ggcgaaccgt
     6721 gcatcaccga cgtgtgtgca gccgtggcgc agttgcgtga gtcaggctgt gatggggtga
     6781 tcgcatttgg cggcggctcg gtgctggatg cggcgaaagc cgtggcgttg ctggtgacga
     6841 acccggatag cacgctggca gagatgtcag aaaccagcgt tctgcaaccg cgcttgccgc
     6901 tgattgccat tccaacgacc gccggaaccg gctctgaaac caccaatgta acggtgatta
     6961 tcgacgcggt gagcgggcgc aagcaggtgt tagcccatgc ctcgctgatg ccggatgtgg
     7021 cgattctcga cgccgcgttg accgaaggtg tgccgtcgca tgtcacggcg atgaccggca
     7081 ttgatgcgtt aacccatgcc attgaagcat acagcgccct gaacgctaca ccgtttaccg
     7141 acagtctggc gattggtgcc attgcgatga ttggcaaatc gctgccgaaa gcggtgggct
     7201 acggtcacga ccttgccgcg cgcgagagca tgttgctggc ttcatgtatg gcgggaatgg
     7261 cgttttccag tgcgggtctt gggttgtgcc acgcgatggc gcatcagccg ggcgcggcgc
     7321 tgcatattcc acacggtctg gcgaacgcca tgttgctgcc aacggtgatg gaatttaacc
     7381 ggatggtttg tcgtgaacgc tttagtcaga ttggtcgggc actgcgaact aaaaaatccg
     7441 acgatcgtga cgctattaac gcggtaagtg agctgattgc ggaagttggg attggtaaac
     7501 gactgggcga tgttggtgcg acatctgcgc attacggcgc atgggcgcag gccgcgctgg
     7561 aagatatttg tctgcgcagt aacccgcgta ccgccagcct ggaacagatt gtcggactgt
     7621 acgcggcggc gcaataaatg ccggatgcga cgcttgccgc gtcttatcca gcctacggga
     7681 ttgcacatgt agggcggata aggcgttcac gccgcatccg ccagtggcgc ggtgcaattg
     7741 ccggatgcga tgcttgccgc gtcttatccg gcctacggga ttgcacatgt agggcggata
     7801 aggcgttcac gccgcatccg ccagtggcgc ggtgcaattg ccggatgcga cgcttgccgc
     7861 gtctgatccg gccttcggga ttgcacatgt agggcggata aggcgtttac gccgcatccg
     7921 ccaataaata acaactaaca gggagtaaag gcgatgggaa ttaacgaaat catcatgtac
     7981 atcatgatgt tctttatgct gatagctgcc gtagacagga tcctgtcgca gttcggcggt
     8041 tctgctcgtt tcctcggtaa gttcggtaaa agtatcgaag gatcaggcgg tcagttcgaa
     8101 gaaggcttta tggcaatggg cgctctgggt ctggcgatgg tcggtatgac cgcgctggca
     8161 ccggtactgg ctcacgtact cgggccggta attatcccgg tttacgaaat gctcggcgca
     8221 aacccgtcaa tgttcgccgg aacgctgctg gcgtgcgata tgggcggctt cttcctcgcc
     8281 aaagagctgg cgggcggcga cgtagcagcg tggctatact ctgggttaat tcttgggtcg
     8341 atgatggggc caacgattgt gttttccatt ccggtggcgc tcggcattat cgaaccttct
     8401 gaccgtcgtt atctggcgct cggcgtgctg gcgggcattg tgaccattcc gattggctgt
     8461 attgctggtg gtctggttgc tatgtactcc ggtgtgcaga tcaacggtca gccggtggaa
     8521 ttcactttcg ccctgatcct gatgaacatg atcccggtga tcattgttgc gattctggtg
     8581 gcgctggggc tgaaattcat cccggaaaaa atgatcaacg gcttccagat cttcgccaaa
     8641 ttcctcgttg cattgatcac cctcggtctt gctgccgcgg tagttaagtt tctgcttggc
     8701 tgggaactga tccccggtct ggaccctatc tttatggccc ctggcgataa acccggtgaa
     8761 gtgatgcgcg ccattgaagt tatcggttct atctcctgcg ttctgttagg ggcgtatcca
     8821 atggtgctgc tgctgacccg ctggtttgaa aaaccgctga tgagcgtcgg taaagtgctg
     8881 aatatgaaca acatcgcggc agccggcatg gtggcaacgc ttgccaacaa catcccgatg
     8941 ttcggcatga tgaagcagat ggatacccgc ggcaaagtca tcaactgcgc cttcgccgtt
     9001 tccgctgctt tcgccctggg cgaccactta ggcttcgccg ctgccaacat gaacgccatg
     9061 atcttcccga tgattgtcgg caagttgatc ggcggcgtaa cggcgattgg cgtggcgatg
     9121 atgctggtgc caaaagaaga cgcgaccacg gctaaaaccg aagcggaggc acaatcgtga
     9181 acactcgcca gctattgagc gtcggtatcg atatcggcac caccaccacc caggtgattt
     9241 tctcccacct ggagctggtt aaccgtgcgg cggtgtcgca ggtgccgcgc tacgaattca
     9301 ttaaacgcga aattagctgg caaagcccgg tgttctttac ccctgtcgat aaacagggtg
     9361 gtttaaaaga agcggaactg aaaaccttaa tcctcgagca atatcaggct gcgggcattg
     9421 cgccggaaag cgttgattct ggtgccatca tcatcaccgg tgaaagcgcg aaaacccgca
     9481 atgctcgtcc ggcggtgatg gtgctctccc agtcgctggg cgattttgtc gttgccagcg
     9541 ccgggccgca cctcgaatcc gtcattgccg gtcacggtgc cggggcacaa accctttctg
     9601 aacaacggct gtgtcgggta ctgaatattg acatcggcgg tggcactgcg aactacgccc
     9661 tgttcgatgc cggaaaaatc agcggcactg cctgcctcaa cgtcggtggt cgcctgctgg
     9721 aaaccgacag ccaggggcgc gtggtttacg ctcataaacc ggggcagatg attgtggatg
     9781 agtgcttcgg cgcaggcacc gacgcccgtt cgctgaccgg cgcgcagctg gtgcaggtta
     9841 cccggcggat ggcagaactg attgtcgaag tgattgacgg aacgctctcg ccgctcgcgc
     9901 aggcattgat gcaaaccggt ttgctgcccg caggtgttac gcccgaaatc attacgcttt
     9961 ctggcggcgt gggcgaatgt tatcgccacc agcccgccga cccgttctgt tttgccgata
    10021 ttggcccgct gctggcaacg gcgctgcatg accatccgcg cctgcgtgag atgaacgtac
    10081 agtttccggc gcaaaccgtg cgcgccacgg tgattggcgc gggcgcgcat accctttcgc
    10141 tctctggcag cacaatctgg cttgagggcg tacaactgcc gctgcgcaat ttgccggtgg
    10201 cgatcccgat tgatgaaacg gatctggtga gtgcctggca acaggcgctg cttcagctgg
    10261 atcttgatcc caaaactgac gcgtacgtgc tggcgcttcc cgcctcgctg cctgtgcgtt
    10321 acgccgcggt actgacggtc atcaacgcgc tggtcgattt cgtcgcgcgt tttccaaatc
    10381 cgcatcccct gctggtggtg gccgggcagg actttggtaa agctctgggc atgttgttgc
    10441 gcccacagct acaacaactc ccgttggcag tcattgacga agtgattgtc cgcgcggggg
    10501 actatatcga cattggtacg cctctttttg gcggatcggt tgtgccggtg acggtgaaat
    10561 cactcgcatt tccttcctga gggaacgact tatgaaacta aagaccacat tgttcggcaa
    10621 tgtatatcag tttaaggatg taaaagaggt gctggctaaa gccaacgaac tgcgttcggg
    10681 ggatgtgctg gcgggcgttg cagcggcaag ctcacaggag cgcgtggcgg caaagcaggt
    10741 gttgtcggaa atgaccgtag cggacatccg caataatccg gtgattgcct atgaagatga
    10801 ctgcgtgacg cggctgattc aggacgatgt taacgaaacg gcctacaacc agattaaaaa
    10861 caggagcatc agcgaactgc gtgagtatgt gctgagcgat gaaaccagcg tggacgacat
    10921 tgcctttacc cgcaaagggc tgacctcgga agtggtcgcg gcggtagcga agatttgctc
    10981 caacgcggac ctgatctacg gcgcgaagaa aatgccggta atcaaaaagg ccaataccac
    11041 catcggtatt ccgggcacct tcagcgcccg tttgcagcca aatgacaccc gtgacgacgt
    11101 gcaaagtatc gctgcacaaa tctacgaagg gctttccttc ggggtgggcg atgcggtgat
    11161 cggcgttaac ccggtaactg acgacgtgga aaacttaagc cgcgtgttgg acaccattta
    11221 cggcgtgatc gacaaattca acatcccaac tcagggctgc gtactggcgc acgtcaccac
    11281 ccagatcgaa gcgattcgtc gcggcgcgcc tggcggactg attttccaga gtatctgtgg
    11341 cagcgaaaaa gggctgaaag agtttggcgt ggaactggcg atgctcgacg aagcgcgcgc
    11401 agtgggtgct gagttcaacc gtatcgccgg ggaaaactgc ctctacttcg aaaccgggca
    11461 aggttcggcg ctgtctgctg gcgctaactt cggcgcagac taggtgacga tggaagcacg
    11521 taactatggg ctggcgcgtc attacgatcc gtttatcgtc aacaccgtgg tcggctttat
    11581 tgggccggag tatctctaca acgaccgcca gattatccgt gctggcttag aagatcactt
    11641 tatgggcaag ctgagcggca tctctatggg ctgtgactgc tgttacacca accacgctga
    11701 tgctgaccag aacctcaacg aaaacctgat gatcctgctc gccaccgcag gctgcaacta
    11761 catcatgggg atgccgctgg gcgatgacat catgctcaac tatcagacca ccgcattcca
    11821 cgacactgcc actgtgcgtc agttactcaa cctgcgcccg tcaccggagt ttgaacgctg
    11881 gctggaaagc atgggcatta tggcaaacgg tcgcctgacc aaacgggcgg gcgatccgtc
    11941 actgttcttc tgatgacgcg gagataacac atcatggatc aaaaacagat tgaagaaatt
    12001 gtacgcagcg tgatggcgtc aatgggacaa gcggcccccg cgccgtcaga agcaaagtgc
    12061 gccaccacta actgtgcggc accggtgacc tcggaaagct gcgcgctgga tttaggttct
    12121 gcggaagcaa aagcgtggat tggtgttgaa aacccgcatc gcgcagacgt attaacagaa
    12181 ctgcgccgca gcaccgtggc ccgcgtttgt accggtcgta ccggtccgcg tccacgtacg
    12241 caggcgctgc tgcgtttcct ggccgatcac tctcgttcga aagataccgt actgaaagaa
    12301 gtgccggaag agtgggtgaa agcgcagggc ttgctggaag tacgctccga aattagcgac
    12361 aaaaatctct acctgactcg cccggatatg ggccgccgcc tgtgtgcaga agctgttgaa
    12421 gcgctgaaag cacagtgtgt tgctaatccg gacgtacagg tcgttatttc tgatggcctg
    12481 tcaactgatg ccatcaccgt gaactacgaa gagatcctgc cgccgctgat ggcgggcctg
    12541 aaacaggccg ggctgaaagt cggcacgcca ttctttgttc gttatggtcg cgtgaagatt
    12601 gaagatcaga tcggtgagat cctcggcgcg aaagtggtga tcctgctggt gggggaacgt
    12661 ccggggttgg gtcagtcaga aagcctctcc tgttacgccg tttactcgcc gcgtatggcg
    12721 accaccgttg aggccgatcg cacctgcatc tctaacattc accagggcgg cacgccgccg
    12781 gttgaagccg cagccgtcat tgtggatttg gcgaaacgta tgctggagca gaaagcatcc
    12841 ggcatcaaca tgacccgata aggaggcatc atgccagctt tagatttgat tcgaccgtcg
    12901 gtaaccgcta tgcgggtgat tgcctccgtt aactctgaat ttgcgcgtga actaaaattg
    12961 ccgccgcata ttcgtagcct cgggctgatt tctgctgatt ccgatgacgt cacctacatt
    13021 gcggctgacg aagcgaccaa gcaggcgatg gtcgaagtgg tatatggccg ctcgctgtat
    13081 gcgggagcag cacacggacc gtcaccgact gccggtgaag tgctgattat gcttggtggg
    13141 ccaaacccgg cggaagtgcg tgctggtctg gatgcgatgg ttgcgcatat tgaaaatggc
    13201 gctgctttcc agtgggctaa tgacgcagaa aacaccgcat tcctggcaca tgtagtttcg
    13261 cgtaccggtt cttatctttc atcaaccgcc gggatcacgc ttggcgatcc gatggcgtat
    13321 ctggtggcac cgccgctgga agcgacctac ggcattgatg cagcgttgaa atctgccgac
    13381 gtacagctgg tgacctatgt cccgccaccg tctgaaacca actactcggc agcattttta
    13441 accggtagcc aggccgcgtg taaagcagcc tgtaacgcct ttaccgatgc agtgctggaa
    13501 atcgcgcgta atccaatcca gcgtgcgtaa cggaggttgc cgatgatcaa tgcactggga
    13561 ttgctggaag tggacggcat ggtcgccgcg atagatgcag cggatgccat gctcaaagca
    13621 gctaacgttc gtctgctcag tcacgaagtg cttgaccctg gtcgcttaac gctggtggtg
    13681 gaaggcgatc tggcggcgtg tcgtgcggcg ctggacgcag gttgtgctgc cgcgatgcgt
    13741 accgggcgtg tcatcagccg caaggagatc ggtcggccag acgatgacac ccagtggctg
    13801 gttactggct ttaaccgcca gccgaagcaa cccgtaaagg aacccgacgc gccagttatc
    13861 gtcgcggaat ctgctgacga gttgttggcg ctgttaacat cagtacgtca gggaatgacg
    13921 gcaggagaag tggctgccca ctttggctgg ccgctggaaa aagccagaaa tgcgctcgaa
    13981 cagctctttt ctgccgggac gttacgtaaa cgcagtagtc gttatcgtct caagccccat
    14041 taacctgtcg gaggtgccgg gtgtcctaca acacccggca ttaacatcat gaaaaagacc
    14101 cgtacagcca atttgcacca tctttatcat gaacccttac ccgaaaacct gaagctcacg
    14161 ccgaaggtcg aagtggataa tgttcatcaa cgacagacaa cggatgtcta tgaacatgct
    14221 ttgacgatta ccgcctggca gcagatttac gatcagctgc atccgggcaa gtttcatggt
    14281 gaatttacgg aaattctgct cgatgatatt caggtttttc gtgaatacac cggtctggcg
    14341 ctgcgtcagt cgtgcctggt ctggccgaac tcgttctggt ttggcattcc ggcgacgcgc
    14401 ggtgagcagg gatttatcgg ttcgcaatgt ctgggaagcg cagaaatagc gacgcgcccc
    14461 ggtggaaccg agtttgaatt aagtacgccg gacgattata cgatcctggg cgtagtgctg
    14521 tctgaagatg tcatcacccg tcaggctaac tttttgcaca atccggatcg ggtattacat
    14581 atgctgcgta gccagtcggc gctggaagtg aaagagcagc ataaagccgc gctgtggggc
    14641 tttgtccaac aggcgctggc gacgttttgc gagaacccgg aaaatctcca tcagcctgca
    14701 gtgcgaaaag tgctggggga taatttgcta atggcgatgg gggcgatgct ggaagacgcg
    14761 caaccaatgg tgacggcgga aagcatcagt catcagagtt accgtagatt actttcccgc
    14821 gcccgtgaat atgtgctgga aaatatgtct gagctggtga cggtgctgga cttgtgtaat
    14881 caactgcatg tcagtcgccg cacgctacaa aacgcgtttc acgctatttt aggcattggc
    14941 ccaaacgcgt ggctgaaacg cattcgcctg aacgccgtac gccgcgaact gataagcccg
    15001 tggtcgcaaa gcacaacggt aaaagacgcc gccatgcagt ggggattctg gcatctgggg
    15061 caatttgcca cggattacca gcagctgttt gccgagaagc cgtcgttgac gttgcatcag
    15121 cggatgcggg aatgggggtg agggagttac acccaatccc tgaccttaat aaactcactt
    15181 aacgccgctt ctgggctgcc atcttttggc tgataatcat actcccagcg taccagtggc
    15241 ggcattgaca tcaggatcga ctcggtgcgc ccgccagttt gcaggccaaa cagagtgccg
    15301 cgatcccaga ccagattgaa ctcgacataa cgaccgcgac ggtagagctg aaaattgcgc
    15361 tcgcgctcgc cgtaggccat cgctttacgt cgctctacaa ttggtaaata agcgtcggtg
    15421 tagcctttgc ctaccgcctg cataaaggca aaacagtggt cgaaatctgg cgtgttcaga
    15481 tcatcaaaga acagcccgcc aataccgcgc tgttcgttgc gatgtttgag gtagaagtaa
    15541 tcgtcgcacc actttttgta acggggataa acgtcttcac caaatggcag gcacaggtca
    15601 cgggcggtgc ggtgccagtg aatggcgtct tcttcaaaac catagaaagg ggttaaatcg
    15661 aagccgccgc caaaccacca gacgggctct gcacccggtt tttcggcaat aaaaaaacgc
    15721 acattcgcgt ggctggtggg aacatacggg ttatgcggat gcactaccag tgaaacgccc
    15781 atcgcctcga aactgcgccc ggcaagttcc gggcgatgag cggtggcgga agcaggcatc
    15841 gcctcgccgt ggacgtgaga aaagttgacg cctgcctgtt cgaaaacacc accattacgc
    15901 aacacccgac tacgcccgcc gccgccagct tcgcgctgcc aactatcttc gacaaattct
    15961 gcgccatcga cggcggtcag ctgctgacaa atcgtatcct gaaggttgag cagaaactgt
    16021 ttaacctggt gtgcgtcggg tttcataact tatcgctttt tcgaatgtgc tttctggttg
    16081 tcgaaccagt ggaaataact gatcacgcct tcagcaatcg ctgtggcgat tttctgacga
    16141 aacgccaccg tgcctaacag ccgctcttct tccgggttgg tgataaacga ggtttccacc
    16201 agcaccgaag gaaccgacgg cgatttcaac accacaaacg cggcttgttc ggtgttgcgg
    16261 ctgtgcagtt tatgcaccgg cttaatcttt ttcagaatat gcgagccaag cgtcaggctg
    16321 tttttaatag tatcggtttg caccagatca aacagcactt gctgcaatag gtgatccttg
    16381 tcagtcgcct ttttaccggc aacttcatcg gcgcggtttt cgcgttcaga caggtatttc
    16441 gccattgcgc tacttgcccc acggttagag agggcaaata ccgaagcacc ggcagctttc
    16501 gggttggtaa agccatcggc atgaattgac ataaacagat ctgcgccatg tttatgggcg
    16561 atttcaacgc gatcgtaaag tgggataaac gtatcgccag aacgcgttaa acgcgcatca
    16621 atcccatgat tacgcaaaat ggaacggacg tttttagcaa tcgccagcac cacatgtttt
    16681 tctttcgaac cgttgcgtcc gatcgctccg gtatcaattc cgccgtggcc tggatcgaga
    16741 acaacgacac gtttgccgcc agattttttg gctttcggct tgctgtgtcc gttgctggtt
    16801 tttaaaggtt cgtctttggc gatggcttgc gacattcctg acaacgtcag ggcagccaaa
    16861 ccggctttca gcacctggcg gcgcgaagtg agtgttttta gtggtttaaa agtgctcata
    16921 cggcctgagt tgtaataata aagttccaga tgttatatcg tatcgcgtaa tccgttacga
    16981 cggtttgatg ttgagaattg ttttactttt catttcaata gttgacaact ccccattatg
    17041 ccactttttc aaccgatttt cgtcagatat tggctaattc gaccgttcac gccataatca
    17101 gccttttaac ccgcaaaaag gtaacgaata ccatggagat acgcgtattt cgccaggaag
    17161 atttcgaaga ggtcatcacc ctttgggagc gttgcgactt gctgcgtccg tggaacgatc
    17221 cggaaatgga catcgagcgt aagatgaacc atgacgtcag tttgtttttg gtcgctgagg
    17281 taaacggtga agtggtcgga acggtgatgg gcggttatga cgggcatcgc gggtctgctt
    17341 attatcttgg cgtgcatcca gagtttcgtg ggcgtgggat tgccaatgcg ttgcttaatc
    17401 ggctggagaa aaagctgatt gctcgtggct gcccgaaaat tcagatcaat gtgccggaag
    17461 ataacgacat ggtgctcgga atgtatgaac gcctgggata tgaacacgcc gacgtgctga
    17521 gtctgggtaa gcgtttgatt gaagatgaag agtactgagt tccatcctgt ccattatgat
    17581 gcgcacggac gcctgcgttt acccttgctc ttctggcttg tgctattgct tcaggcgcga
    17641 acctgggtgc tgtttgtcat tgccggtgcg tcgcgcgagc agggtactgc gctgctaaat
    17701 ctgttttatc ccgatcacga taatttctgg ctggggctga ttccaggcat tccggcggtg
    17761 ctggcgtttt tgttgagcgg tcggcgggct acatttcctc gcacttggca tgtgctctat
    17821 tttctgttgc tgttggcgca ggtggtttta ctttgctggc aaccgtggct gtggctgaac
    17881 ggtgaatccg ttagcggtat cggtctggcg ctggtagtgg cggatattgt ggcgttaatc
    17941 tggctgctga ccaatcgacg tttacgcgct tgttttaatg aagaaaaaga ataacggcac
    18001 tttttggtga atttgcactc caagcaacgt tattgaataa ccaaaggaag tgacatgaaa
    18061 tcgctgcgtt taatgttatg cgctatgccg ttgatgctga ccggctgttc cacgatgtcg
    18121 tcagttaact ggtcggccgc taacccgtgg aactggtttg gatcgtccac caaagtgagc
    18181 gagcagggcg tgggtgaatt aacggcgtcc acaccactgc aagaacaagc cattgctgat
    18241 gcgcttgatg gcgattatcg cctgcgcagc ggaatgaaaa ccgcgaacgg caacgtggtg
    18301 cgcttttttg aagtgatgaa aggcgacaac gtggcgatgg tgattaacgg cgatcaaggc
    18361 acgatcagtc gcattgacgt gctggatagc gatattcctg ctgacaccgg tgttaaaatc
    18421 ggtacaccgt ttagcgacct ttacagcaaa gcatttggca attgccaaaa agctgatggt
    18481 gatgataatc gtgccgtcga atgtaaagcc gaaggcagtc aacatattag ctaccagttc
    18541 agcggggaat ggcgtggtcc tgaagggtta atgccttcgg acgataccct gaaaaactgg
    18601 aaagtcagta aaattatctg gcggcgttaa tttgcgtctg aacaaaccgc cgccgcgaaa
    18661 aaacacgtaa aatagcgctc aacaatgcca cggattgcgt ggcattctta ttttcaggag
    18721 gaacaatgtc tcaggttcag agtggcattt tgccagaaca ttgccgcgcg gcgatttgga
    18781 tcgaagccaa cgtgaaaggg gaagttgacg ccctgcgtgc ggccagtaaa acatttgccg
    18841 acaaactggc aacttttgaa gcgaaattcc cggacgcgca tcttggtgcg gtggttgcct
    18901 ttggtaacaa cacctggcgc gctctgagcg gcggcgttgg ggcagaagag ctgaaagatt
    18961 ttccgggcta cggtaaaggc cttgcgccga ccacccagtt cgatgtgttg atccacattc
    19021 tttctctgcg tcacgacgta aacttctctg tcgcccaggc ggcgatggaa gcctttggtg
    19081 actgcattga agtgaaagaa gagatccacg gcttccgttg ggtggaagag cgtgacctga
    19141 gcggctttgt tgacggtaca gaaaacccgg cgggtgaaga gacgcgtcgc gaagtggcgg
    19201 ttatcaaaga cggcgtggat gccggcggca gctatgtgtt tgtgcagcgt tgggagcaca
    19261 acctgaagca gctcaaccgg atgagcgttc acgatcagga gatgatgatc gggcgcacca
    19321 aagaggccaa cgaagagatt gacggcgacg aacgtccgga aacctctcac ctcacccgcg
    19381 ttgatctgaa agaagatggc aaagggctga agattgttcg ccagagcctg ccgtacggca
    19441 ccgccagtgg cactcacggt ctgtacttct gcgcctactg cgcgcgtctg cataacattg
    19501 agcagcaact gctgagcatg tttggcgata ccgatggtaa gcgtgatgcg atgttgcgtt
    19561 tcaccaaacc ggtaaccggc ggctattact tcgcgccgtc gctggacaag ttaatggcgt
    19621 tgtaagcgtt tctgattcga tgattaaagg ccagccagta acaaaactga ctggcctttt
    19681 tattactttt gcttcaacgc cgcatacacc tgatcaacta cccaaccata agttgcaatc
    19741 ggtaactgac cgctttcgaa catattggga tttttttgtg gatcggcaac tggcgaatgg
    19801 ggcttgttgc ttaacatcac aatcgccata tgattgaccg gatcgataac ggttactgtc
    19861 ccggtccagc cagtgtgacc gtaggtttgc gggcttgcca gcgtgccaaa cgtcggcgtc
    19921 atggtggcat taccattcac gcgccagccg aggccaaaag tggcatcttc cttagagctg
    19981 gtggtgaaca tcttcaccgt ttccgcactg aacagctgca catcgccata gcccccgccg
    20041 ttcagcatcg tttgcattaa caccgcaata tcgccggtat tggaaaacaa acctgcgtgc
    20101 ccggaaacgc cgcccatcga ataaaaggct ttttcatcgt gcacctgacc ccagagagtg
    20161 gaggtgcgga tattcgggaa atggatcacg ccatcgcggg tattgccgtt caattccgtg
    20221 gcggcaattt gttgcggttt aaagcctttc agcagcgggt taaataccgt atgtgtcagg
    20281 ccgagcgggc gataaatcga ctcttcaaca tagcggtcaa gcggctgacc ggtaaccgac
    20341 tcgacgataa atccaagcag catataatcg acatcgctgt agatatgttt gctgccgggc
    20401 tgatattcca gcggcgtgcg cttgatcatc tccagcgtct ggcctttatc ctgggaatat
    20461 aacgcgcccg cgaccgcttt attcgggtat tgcggatctg ccgggaaacc gccgctgtga
    20521 tgcagcaggt cagaaatccg cagggtgttt ttgcctttaa tggtgtcatt tggactatcg
    20581 gcaaaccccg gaatatattt cgcaatccga tcgtcaggat gcagtttgcc ttcgctcatc
    20641 agcttttgca gggcgaagtt cgtggcgtac attttggtgt ttgaggccag gtcatacagc
    20701 gtcccggtgg tggctttgac aggctgttcc atcaacacgc tgccatcgta ctttttcgcc
    20761 gctccccagg ctttgcgata cacaatctga ttatctttaa tgatcagcag gtttacgccg
    20821 ggataaccga catcaatttg ctggctaatc cagcgatcca tctggttaag ccgttcgacg
    20881 ttaaaccctg ctttctctgg cgagctttct gtcagaacag ggtattttgc ggcgctgacg
    20941 ctacaactaa cagccagcag agaaagatag agcattgtcc gtttcatgat atcagtccag
    21001 attgacgtta cggcagccaa agagcgtggt gaaaataaac ccgcaaaccc atgccaccag
    21061 aatcccgcca gcataaaccg ccatggccgg aaggatgcct tgtgcggaag tcatcagcgg
    21121 cagcgctacc agaccagacg gcccaaaggc gctgtttaag cccatcggca gaccccatca
    21181 ggctatcagg ccgataaaca aaccgcccgc cgcgccgcct aaacaggcgg taacaaacgg
    21241 cttcatgcgg ggcagggtga caccgtaaat cagcggttca ccaacgccca gcaggccggg
    21301 aataatcgcc ccgcgtacct gactgcgtag cgcactgtgc ggttgcgccc gccagtagag
    21361 tgccagcgcc gcgcccacct ggcccgcgcc tgccattgaa aggatgggaa ataagctgtt
    21421 gaatccctag ctgtccatta acgcgaggta aacaggaata aagccctgat gcacgccaaa
    21481 taccacggcg atcaggaaca gcccggctaa aaccgcacaa ccgaacggat tactgttcag
    21541 gtgcataaac agccatgaca taccttcgaa taaccagcca cccagcggca taatgatcag
    21601 gtacgcaagc gtagcggtga tcagcagggt gattaacgag gtcagcagca tgtcgagatc
    21661 gtccgacata aagcggcgca ccatgccttc aatgcgggcg caggcccacg cggcaatcag
    21721 cacgccgata atattgccgc gcggatcgat gggcagacca aagaaatcgt gaaaaccggc
    21781 gtagtcaccg gtggttgcag cggggttata accgagcaaa aagagtgcgg caataattgc
    21841 gccattgacg cccgtgccgc caaatgcctg ggcggcgtta tagcccacca gaatcaccag
    21901 gaaagtgaac aaacctttgc tgaacacctt cataaaattc agcgcgtcgg gtagcgtgcc
    21961 ctgagcatct gccggaacgt gcatcaccgt ggcaattaac gtcgctatcc ccagcagcag
    22021 accggcggca ataaaaccgg ggatcagcgg cgtgaagatg gtggcgaatt tggcgagaaa
    22081 ttgttgcacg ccggaggttt gtttggcttt taactgacgt ttgttctggg cggcgatttc
    22141 tgcggcatcc tgtaccggtg cctcgcccag cagctcgctc atcgcttttg cggcacgatg
    22201 cgctttacca ggcccaaaaa cgacctgcac ctggtcgctg gtcaaaatga cgcccttcac
    22261 gccttccagc gttttgatat tgggatcaac cagtgaactg tcatgtacac ccagacgcag
    22321 gtgcgtcata cagttaccac aactggcgat atttcccagt ccgccgacac gggtaagaat
    22381 ggtgttcaga agttcactgc tgatctcttt ggccatgggt tattccttgt ctaaaacctg
    22441 acgaataaag ccgccgtgtt gatccaggcg ttttttagct tctgcggcat cgagattttt
    22501 cagcaccatc acaatggccg ttttacagtt gcgctcgcag gcaattaacg ccgcttccgc
    22561 ttgttcggca ttacatccgg tggcgttttt aacgatattg acctggcgca catgcagttt
    22621 ttcgttggtg gcgaccacat cgaccatcag gttgccgaac actttgccgg atttaatcat
    22681 cagcccggtg gaaagcatat tgagcaccag tttctgcgct gtgcctgctt tcattcgcga
    22741 agaaccagtg accacttcgg cacctacaat cggtgtaatg gcaaactcgg cggtggtgga
    22801 aacggcactc cccggattac aggaaattcc cactgtgcgg caaccgagct ggcgggcgta
    22861 ttccagtccg gcaatcacat acggcgtacg accgctggcg gcaataccga ccaccacatc
    22921 ctgtgccgtt aaatttatat ttttcagatc gttaatgccg ccttctcggc tatcttccgt
    22981 gccttccacc gcgtgctgaa tggcatattc gccgccagca atcaaaccaa ccaccagacc
    23041 cggtttcacg ccgtaggtgg gcggacattc gctggcatcc agaatcccca gacgaccgga
    23101 tgttcccgca ccgagataaa tcagacgacc gccgccgctt acctgggcgt ggataacatc
    23161 gatcgccgcg gcgatatccg gcaggacgcg ctcaacggca agcggtacgg ttttatcttc
    23221 atcgttgata atccggcaca tttccagcgt cgatacgcgg tcaatttcag ccgaggcggt
    23281 gttcgagcct tcagtaatca tcttttcaag ttgcatcggt ggtgacctta ctatttacga
    23341 tcaaataatt gcagatgtat ggaatcatat atcccgaatt gacagactta aaggaatcat
    23401 tgagtctaca cttgcagagt gtgaagagcg tcataagaac ttaagcggaa tttttgctgc
    23461 cagcccggga gagtggaaga tgttgtacct gacgaaaata cgcaatgcgg aaagtgaatt
    23521 taccgagaac gagcaaaaaa ttgctgactt tctgcgggcc aacgtgagtg agctgaaatc
    23581 cgtctcttcg cgcaagatgg cgaaacagct cggcatcagt cagtcgagca ttgtgaaatt
    23641 tgcccagaag ctcggcgcgc aaggatttac tgaattgcgt atggcgttaa ttggcgaata
    23701 cagtgccagc cgggagaaaa cgaatgccac ggcgctgcac ctgcacagtt cgattaccag
    23761 tgatgattcg ctggaagtga ttgcccgcaa actgaatcgc gaaaaagagc tggcgctgga
    23821 gcaaacctgt gcgttgttcg actacgctcg gctgcaaaaa atcattgagg tcatcagtaa
    23881 agcaccgttt atccagataa cgggcctggg cgggtcggcg ctggtggggc gcgatctgtc
    23941 gttcaaactg atgaaaattg gctatcgcgt ggcctatgaa gccgacacac acgtacaagc
    24001 caccgtttcc caggcgttga aaaaaggcga tgtacaaatc gctatctctt acagcggcag
    24061 taaaaaagag attgtcttgt gcgcagaagc ggcacgaaag cagggggcaa cggtgattgc
    24121 cattacttct ctggctgact ctccgctgcg gcggctggcg catttcacgc tcgataccgt
    24181 ttccggtgaa accgaatggc gtagctcgtc gatgtctacc cgcacggcgc aaaattctgt
    24241 taccgattta ctgtttgttg gtcttgtgca actcaatgat gtggagtcat tgaaaatgat
    24301 tcagcgcagc agtgagctaa cacaacgcct gaaatgatgc ataaagcagc gactggattg
    24361 agattttcct gaattagtga gctgatccgc agcaatattt tgtttatcct gtattttcag
    24421 agggaatgga gtgtaacgct ctgtattaac aaggagagca ttaaaatggg taaactcacg
    24481 ggcaagacag cactgattac gggcgcattg cagggaattg gcgaaggaat tgccagaact
    24541 tttgcgcgtc atggcgcaaa cctaatcttg ctggatatct cccctgagat cgaaaagctg
    24601 gcggacgaac tgtgtggtcg tggtcatcgc tgtacggcgg ttgtcgccga tgtgcgtgac
    24661 ccggcgtcgg tagccgcagc tatcaaacgc gcgaaggaaa aagaagggcg cattgatatc
    24721 ctggtgaata acgcaggcgt ttgtcgtctg ggcagtttcc tcgatatgag cgatgacgat
    24781 cgcgatttcc atattgacat caatattaaa ggcgtatgga acgtcacgaa ggcggtgctg
    24841 ccggagatga ttgcccgcaa agatggtcgc attgtgatga tgtcttcagt cactggtgat
    24901 atggtggccg atcctggcga aacggcgtac gccttaacga aagcggcgat tgttggcctg
    24961 acaaaatcgc tggcggtgga gtacgcgcag tctggtattc gcgttaacgc catttgcccg
    25021 ggatacgtgc gcacaccaat ggcggaaagc attgcccgcc agtcgaaccc ggaagatcca
    25081 gagtcggtgc tgactgaaat ggcgaaagca atcccgctgc gtcgcctcgc cgatccgctg
    25141 gaagtcggcg aactggcggc cttcctcgca tcggatgaat ccagctattt aaccggtaca
    25201 cagaatgtga ttgatggcgg cagcacactg ccggagacgg ttagcgtcgg tatctgattc
    25261 acctctgttt cctccctgca tttgtgggga ggatttcgtt ttaaacccgg ttcttattcc
    25321 cttttcaact tccaaatcac caaacggtat ataaaaccgt tactcctttc acgtccgtta
    25381 taaatatgat ggctattaga aagtcattaa atttataagg gtgcgcaatg gccgttaact
    25441 tactgaaaaa gaactcactc gcgctggtcg cttctctgct gctggcgggc catgtacagg
    25501 caacggaact gctgaacagt tcttatgacg tctcccgcga gctgtttgcc gccctgaacc
    25561 cgccgtttga gcaacaatgg accaaagaaa acggcggcga caaactgaca ataaaacaat
    25621 ctcatgccgg gtcatcaaaa caggcgctgg caattttgca gggcttaaaa gcagacgttg
    25681 tcacttataa ccaggtgact gacgtacaaa tcctgcatga caaaggcaag ctgatcccgg
    25741 cggactggca aacgcgcctg ccaaataata gctcgccatt ctactccacc atgggcttcc
    25801 tggtgcgtaa gggcaacccg aagaatatcc acgactggaa cgacctggtg cgctccgatg
    25861 tgaagctgat tttcccgaac ccgaaaacgt cgggtaacgc gcgttacacc tatctggcgg
    25921 catggggcgc agcggataaa gctgacggtg gcgataaagc caaaaccgaa cagtttatga
    25981 cccagttcct gaaaaacgtt gaagtgttcg atactggcgg tcgtggcgcg accaccactt
    26041 ttgccgagcg cggcctgggc gatgtgctga tcagcttcga gtcggaagtg aacaacatcc
    26101 gtaaacagta tgaagcgcag ggttttgaag tggtgattcc gaaaaccaac attctggcgg
    26161 aattcccggt ggcgtgggtt gataaaaacg tgcaggccaa cggtacggaa aaagccgcca
    26221 aagcctacct taactggctc tacagcccgc aggcgcaaac catcatcacc gactattact
    26281 accgcgtgaa taacccggaa gtcatggata aactgaaaga caaattcccg cagaccgagc
    26341 tgttccgcgt ggaagacaaa tttggctcct ggccggaagt gatgaaaacc cacttcacca
    26401 gcggcggcga gttagacaag ctgttagcgg cggggcgtaa ttaatgtttg cagtttcatc
    26461 aagacgcgtg ctgccgggct ttaccttaag cctcggcacc agtctgctgt ttgtgtgcct
    26521 gattttgctg ctgccgctct ccgcgctggt gatgcagctg gctcagatga gctgggcgca
    26581 gtactgggag gtgatcacca acccgcaggt agtagcggcc tacaaagtaa cgctgctgtc
    26641 agcgtttgtg gcatcgattt ttaacggcgt ttttggcctg ctgatggcgt ggatcttaac
    26701 ccgctatcgc tttccgggcc gcacgctgct tgatgcgctg atggatttac cctttgcgct
    26761 gccaacggca gtcgccggtt taacgctagc ctcgctcttt tctgtaaacg gtttttacgg
    26821 tgaatggctg gcgaagtttg atatcaaagt cacctatacc tggctgggga ttgcggtggc
    26881 gatggccttt accagcattc cgtttgtggt acgtaccgtg cagccggtgc tggaagagtt
    26941 aggcccggaa tatgaagaag cggcagaaac gcttggcgca acgcgctggc agagtttctg
    27001 caaagtggtg ctgccggaac tttctccggc gctggtggcg ggcgtggcgc tgtcgtttac
    27061 ccgtagtctt ggtgaatttg gcgcggtgat ttttatcgcc ggaaatatcg cgtggaagac
    27121 ggaagtgacg tcgctgatga tttttgtgcg cttacaggag tttgattacc cggcagcgag
    27181 cgcgattgct tcggtgatcc tcgcggcatc tctgctgctg ctgttctcaa ttaacactct
    27241 gcaaagtcgc tttggtcggc gtgtggtagg tcattaatgg cggaagttac ccaattgaag
    27301 cgttatgacg cgcgcccgat taactggggc aaatggtttc tgattggcat cgggatgctg
    27361 gtatcggcgt tcatcctgct ggtgccgatg atttatatct tcgtacaggc gttcagcaaa
    27421 gggctgatgc cggttttaca gaatctggcc gatccggaca tgctgcacgc catctggctg
    27481 acggtgatga tcgcgctgat tgccgtaccg gtaaacctgg tgttcggcat tctgctggcc
    27541 tggctggtga cgcgctttaa cttccctgga cgccagttac tgctgacgct actggacatt
    27601 ccgtttgccg tatcgccggt ggttgccggt ctggtgtatc tgctgttcta cggctctaac
    27661 ggcccgctgg gcggctggct ggatgaacat aatctgcaaa ttatgttctc ctggcccgga
    27721 atggtgctgg tcaccatctt tgtgacgtgt ccgtttgtgg tgcgcgagct ggtgccggtg
    27781 atgttaagcc agggcagcca ggaagacgaa gcggcgattt tgcttggcgc gtctggctgg
    27841 cagatgtttc gccgcgtcac attgccgaac attcgctggg cgctgcttta tggcgtggtg
    27901 ttgaccaacg cccgcgcaat tggcgagttt ggcgcggtgt cggtggtttc cggctcgatt
    27961 cgcggcgaaa ccctgtcgct gccgttacag attgaattgc tggagcagga ctacaacacc
    28021 gtcggctcct ttaccgctgc ggcactgtta acgctgatgg cgattatcac cctgttttta
    28081 aaaagtatgt tgcagtggcg cctggagaat caggaaaaac gcgcacagca ggaggaacat
    28141 catgagcatt gagattgcca atattaagaa gtcgtttggt cgcacccagg tgctgaacga
    28201 tatctcactg gatattcctt caggtcagat ggtcgcgttg ctggggccgt ccggttccgg
    28261 gaaaaccacg ctgctgcgca ttatcgccgg gctggagcat caaaccagcg ggcatattcg
    28321 cttccacggc accgacgtca gccgcctgca cgcgcgcgat cgcaaggtcg gtttcgtgtt
    28381 ccagcattac gcgctgttcc gccatatgac ggtattcgac aatatcgctt ttggcctgac
    28441 ggtgctgccg cgtcgcgatc gcccgaatgc cgcagccatc aaagcgaaag tgacaaaatt
    28501 gctggagatg gtgcagcttg ctcatctggc ggatcgttat ccggcacagc tttccggcgg
    28561 ccagaaacag cgcgtggcgc tggcgcgcgc gctggctgta gaaccgcaaa ttctgctgct
    28621 tgatgaaccg tttggcgcgc tggatgcgca ggtgcgtaaa gagctgcgtc gctggctgcg
    28681 tcaactgcat gaagagctga aattcaccag cgtgttcgtg acccacgacc aggaagaagc
    28741 gacggaagta gctgatcgtg tagtggtgat gagccagggc aatattgaac aggctgatgc
    28801 gccggagcag gtatggcgcg aaccagcgac ccgttttgtg ctcgaattta tgggcgaagt
    28861 gaaccgcctg cagggaacca ttcgcggcgg gcagttccat gttggcgcac atcgctggcc
    28921 gctgggctac acacctgctt atcaggggcc ggtggatctc ttcctgcgcc cgtgggaagt
    28981 ggatatcagc cgccgtacca gtctcgattc gccgctgccg gtacaggtac tggaagccag
    29041 cccgaaaggt cactacaccc aattagtggt gcagccgctg gggtggtaca acgaaccgct
    29101 gacggtcgtg atgcatggcg acgatgcccc gcagcgtggc gagcgtttat tcgttggtct
    29161 gcaacatgcg cggctgtata acggcgacga gcgtatcgaa acccgcgatg aggaacttgc
    29221 tctcgcacaa agcgcctgat aggttgagtg aatgttaaac gcccggaggc gcttcccgcg
    29281 atccgggctt tttaatggca aggtttgtaa cctgtagacc tgataagacg cgcaagcgtc
    29341 gcatcaggca acaccacgta tggatagaga tcgtgagtac attagaacaa acaataggca
    29401 atacgcctct ggtgaagttg cagcgaatgg ggccggataa cggcagtgaa gtgtggttaa
    29461 aactggaagg caataacccg gcaggttcgg tgaaagatcg tgcggcactt tcgatgatcg
    29521 tcgaggcgga aaagcgcggg gaaattaaac cgggtgatgt cttaatcgaa gccaccagtg
    29581 gtaacaccgg cattgcgctg gcaatgattg ccgcgctgaa aggctatcgc atgaaattgc
    29641 tgatgcccga caacatgagc caggaacgcc gtgcggcgat gcgtgcttat ggtgcggaac
    29701 tgattcttgt caccaaagag cagggcatgg aaggtgcgcg cgatctggcg ctggagatgg
    29761 cgaatcgtgg cgaaggaaag ctgctcgatc agttcaataa tcccgataac ccttacgccc
    29821 attacaccac taccgggccg gaaatctggc agcaaaccgg cgggcgcatt acccattttg
    29881 tctccagcat gggaacaacc ggcactatca ccggcgtttc acgctttatg cgcgaacaat
    29941 ccaaaccagt aacgattgtt ggcctgcagc cggaagaagg cagcagtatt cccggcattc
    30001 gccgctggcc tgcggaatat ctgccgggga ttttcaacgc ttctctggtg gatgaggtgc
    30061 tggatattca tcagcgcgat gcagaaaaca ccatgcgcga actggcagtg cgggaaggaa
    30121 tattctgtgg cgtcagctcc ggcggcgcgg ttgccggagc actgcgggtg gcaaaagcta
    30181 accctggtgc ggtggtggta gcgatcatct gcgatcgtgg cgatcgctac ctttccaccg
    30241 gggtgtttgg ggaagagcat tttagtcagg gggcggggat ttaagattat tgcgcaggca
    30301 ccgttttatc cgtctgcgca attaaggcat gtaaaaaagg ctgtgcgttc tcatcgctct
    30361 taccggggat cttcacaata tacggcttcc cggtaatcga tgacgacgaa gcaaccttat
    30421 caataaactg ctcggcagtg tcaatgcggt tacgggtatt gccgagcttc agacgcagat
    30481 gagaaaccgc ttcatcgcag gtatgttcat caccgttgcg cacaaatatc aaatcctttt
    30541 tctgtgctaa tccctccagc atggcgttga tgcgggcttc ttcatgaaca gttaacttcg
    30601 cgtaagcggg gagtgtcatt agtagtgtaa tgaccagaca aatgattttc ttcattgaat
    30661 taccttatcc ttgttttgtg atagccgatt tgactgccag aatcaggtaa agtttcggtt
    30721 ggaatacgat gaggatcatc ggcaaggaga aaaaattaaa tgagtagttt gttgttgttt
    30781 aacgataaga gtagggcgct gcaggcggat atcgtcgccg tgcagtcgca ggtagtgtac
    30841 ggcagcgtag gcaacagcat tgcggtgcct gctataaaac agaacggctt gaatgtcttt
    30901 gccgtgccga cggtattgct gagcaatacg ccgcattatg acactttcta cggtggtgcg
    30961 attccggacg aatggtttag cggctatttg cgtgcgcttc aggagcgtga tgcgctgcgc
    31021 caacttcgtg ctgtaaccac gggctatatg ggaacggcat cgcaaatcaa aatccttgcc
    31081 gagtggctga ctgcgctacg caaagaccat cctgacctat tgatcatggt cgatccggtg
    31141 attggcgata ttgatagcgg aatttatgtc aaacctgacc ttcccgaggc gtatcgacaa
    31201 tatttactgc cgctggcgca aggaattacc cccaatatct ttgagctgga aatcctgacc
    31261 ggtaaaaatt gccgcgatct cgacagtgcc attgctgccg caaaaagtct gctttcagac
    31321 acattaaaat gggtggtgat taccagcgcc tccggtaatg aagaaaatca ggagatgcag
    31381 gtggtggtgg tcagtgccga cagcgtgaat gtcatttccc attcacgggt aaaaaccgac
    31441 ctgaaaggga ctggcgacct gttttgtgct cagcttatca gtggtttgct gaaagggaag
    31501 gcgttaaacg atgcggtgca ccgtgcgggg ttgcgcgtac tggaagtgat gcgctatacc
    31561 cagcagcatg agagcgatga attgattttg ccgccgctgg cggaagcata aaaaatggcg
    31621 ccgatgg
//
LOCUS       DAZPJR010000034        31227 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPJR010000034 DAZPJR010000000
VERSION     DAZPJR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31227
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.035"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..559)
                     /locus_tag="ACHVB7_001443"
     CDS             complement(<1..559)
                     /locus_tag="ACHVB7_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR1913467.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHVB7_001444"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHVB7_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR1913468.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHVB7_001445"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHVB7_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR1913469.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHVB7_001446"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHVB7_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR1913470.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="ACHVB7_001447"
     CDS             complement(4001..6322)
                     /locus_tag="ACHVB7_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR1913471.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHVB7_001448"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHVB7_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR1913472.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="ACHVB7_001449"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="ACHVB7_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR1913473.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="ACHVB7_001450"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="ACHVB7_001450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR1913474.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHVB7_001451"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHVB7_001451"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR1913475.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="ACHVB7_001452"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="ACHVB7_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHVB7_001453"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHVB7_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR1913476.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHVB7_001454"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHVB7_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR1913477.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHVB7_001455"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHVB7_001455"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR1913478.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHVB7_001456"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHVB7_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR1913479.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="ACHVB7_001457"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="ACHVB7_001457"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR1913480.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHVB7_001458"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHVB7_001458"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR1913481.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="ACHVB7_001459"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="ACHVB7_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR1913482.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="ACHVB7_001460"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="ACHVB7_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="ACHVB7_001461"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="ACHVB7_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR1913483.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="ACHVB7_001462"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="ACHVB7_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR1913484.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHVB7_001463"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHVB7_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR1913485.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="ACHVB7_001464"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="ACHVB7_001464"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR1913486.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="ACHVB7_001465"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="ACHVB7_001465"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR1913487.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="ACHVB7_001466"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="ACHVB7_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR1913488.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="ACHVB7_001467"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="ACHVB7_001467"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1913489.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="ACHVB7_001468"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="ACHVB7_001468"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR1913490.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="ACHVB7_001469"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="ACHVB7_001469"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR1913491.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="ACHVB7_001470"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="ACHVB7_001470"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR1913492.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
BASE COUNT         7309 a         7971 c         8449 g         7498 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggtagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgcca
//
LOCUS       DAZPJR010000035        30153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPJR010000035 DAZPJR010000000
VERSION     DAZPJR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30153
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.036"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHVB7_001471"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHVB7_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR1913493.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHVB7_001472"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHVB7_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR1913494.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHVB7_001473"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHVB7_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR1913495.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHVB7_001474"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHVB7_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR1913496.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHVB7_001475"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHVB7_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR1913497.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHVB7_001476"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHVB7_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR1913498.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHVB7_001477"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHVB7_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR1913499.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHVB7_001478"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHVB7_001478"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR1913500.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHVB7_001479"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHVB7_001479"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR1913501.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHVB7_001480"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHVB7_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR1913502.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHVB7_001481"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHVB7_001481"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR1913503.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHVB7_001482"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHVB7_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR1913504.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHVB7_001483"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHVB7_001483"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR1913505.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHVB7_001484"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHVB7_001484"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR1913506.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHVB7_001485"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHVB7_001485"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR1913507.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /gene="rsd"
                     /locus_tag="ACHVB7_001486"
     CDS             complement(18516..18992)
                     /gene="rsd"
                     /locus_tag="ACHVB7_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR1913508.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHVB7_001487"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHVB7_001487"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR1913509.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHVB7_001488"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHVB7_001488"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR1913510.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHVB7_001489"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHVB7_001489"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR1913511.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHVB7_001490"
     CDS             21688..22278
                     /locus_tag="ACHVB7_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR1913512.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHVB7_001491"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHVB7_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR1913513.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHVB7_001492"
     CDS             22750..23445
                     /locus_tag="ACHVB7_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR1913514.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHVB7_001493"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHVB7_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR1913515.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHVB7_001494"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHVB7_001494"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR1913516.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHVB7_001495"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHVB7_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR1913517.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHVB7_001496"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHVB7_001496"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR1913518.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHVB7_001497"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHVB7_001497"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR1913519.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7058 a         7864 c         8306 g         6925 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agggtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg acggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca ggcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagtcgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc tagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac tcaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccaa gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaaatcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaa
//
LOCUS       DAZPJR010000036        29548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPJR010000036 DAZPJR010000000
VERSION     DAZPJR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.037"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHVB7_001498"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHVB7_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR1913520.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHVB7_001499"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHVB7_001499"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR1913521.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHVB7_001500"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHVB7_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR1913522.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHVB7_001501"
     CDS             complement(3644..4030)
                     /locus_tag="ACHVB7_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR1913523.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHVB7_001502"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHVB7_001502"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR1913524.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHVB7_001503"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHVB7_001503"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR1913525.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHVB7_001504"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHVB7_001504"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR1913526.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHVB7_001505"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHVB7_001505"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR1913527.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHVB7_001506"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHVB7_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR1913528.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHVB7_001507"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHVB7_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR1913529.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHVB7_001508"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHVB7_001508"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR1913530.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHVB7_001509"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHVB7_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR1913531.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHVB7_001510"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHVB7_001510"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR1913532.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHVB7_001511"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHVB7_001511"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR1913533.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHVB7_001512"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHVB7_001512"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1913534.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHVB7_001513"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHVB7_001513"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR1913535.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHVB7_001514"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHVB7_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR1913536.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHVB7_001515"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHVB7_001515"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR1913537.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHVB7_001516"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHVB7_001516"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR1913538.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHVB7_001517"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHVB7_001517"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR1913539.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHVB7_001518"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHVB7_001518"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR1913540.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHVB7_001519"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHVB7_001519"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR1913541.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHVB7_001520"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHVB7_001520"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR1913542.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHVB7_001521"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHVB7_001521"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1913543.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHVB7_001522"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHVB7_001522"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR1913544.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7120 c         8160 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZPJR010000037        29393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPJR010000037 DAZPJR010000000
VERSION     DAZPJR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.038"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            55..756
                     /locus_tag="ACHVB7_001523"
     CDS             55..756
                     /locus_tag="ACHVB7_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1913545.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            781..3380
                     /locus_tag="ACHVB7_001524"
                     /pseudo
     CDS             781..3380
                     /locus_tag="ACHVB7_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3371..4441
                     /locus_tag="ACHVB7_001525"
     CDS             3371..4441
                     /locus_tag="ACHVB7_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1913546.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4453..4995
                     /locus_tag="ACHVB7_001526"
     CDS             4453..4995
                     /locus_tag="ACHVB7_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1913547.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5003..5518
                     /locus_tag="ACHVB7_001527"
     CDS             5003..5518
                     /locus_tag="ACHVB7_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1913548.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5484..6220
                     /locus_tag="ACHVB7_001528"
                     /pseudo
     CDS             5484..6220
                     /locus_tag="ACHVB7_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6331..7341
                     /gene="pyrD"
                     /locus_tag="ACHVB7_001529"
     CDS             6331..7341
                     /gene="pyrD"
                     /locus_tag="ACHVB7_001529"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR1913549.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7515..8057
                     /gene="zapC"
                     /locus_tag="ACHVB7_001530"
     CDS             7515..8057
                     /gene="zapC"
                     /locus_tag="ACHVB7_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR1913550.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8054..9163)
                     /gene="ycbX"
                     /locus_tag="ACHVB7_001531"
     CDS             complement(8054..9163)
                     /gene="ycbX"
                     /locus_tag="ACHVB7_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR1913551.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9407..11515
                     /gene="rlmKL"
                     /locus_tag="ACHVB7_001532"
     CDS             9407..11515
                     /gene="rlmKL"
                     /locus_tag="ACHVB7_001532"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR1913552.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11527..13434
                     /locus_tag="ACHVB7_001533"
     CDS             11527..13434
                     /locus_tag="ACHVB7_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1913553.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISHLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQMADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13564..14817
                     /gene="pqiA"
                     /locus_tag="ACHVB7_001534"
     CDS             13564..14817
                     /gene="pqiA"
                     /locus_tag="ACHVB7_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR1913554.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14822..16462
                     /gene="pqiB"
                     /locus_tag="ACHVB7_001535"
     CDS             14822..16462
                     /gene="pqiB"
                     /locus_tag="ACHVB7_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR1913555.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16459..17022
                     /gene="pqiC"
                     /locus_tag="ACHVB7_001536"
     CDS             16459..17022
                     /gene="pqiC"
                     /locus_tag="ACHVB7_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR1913556.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17278..17445
                     /gene="rmf"
                     /locus_tag="ACHVB7_001537"
     CDS             17278..17445
                     /gene="rmf"
                     /locus_tag="ACHVB7_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR1913557.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17515..18033)
                     /gene="fabA"
                     /locus_tag="ACHVB7_001538"
     CDS             complement(17515..18033)
                     /gene="fabA"
                     /locus_tag="ACHVB7_001538"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR1913558.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18102..19862)
                     /locus_tag="ACHVB7_001539"
     CDS             complement(18102..19862)
                     /locus_tag="ACHVB7_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1913559.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20048..20500
                     /gene="matP"
                     /locus_tag="ACHVB7_001540"
     CDS             20048..20500
                     /gene="matP"
                     /locus_tag="ACHVB7_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR1913560.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20576..21616)
                     /gene="ompA"
                     /locus_tag="ACHVB7_001541"
     CDS             complement(20576..21616)
                     /gene="ompA"
                     /locus_tag="ACHVB7_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR1913561.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21971..22480)
                     /gene="sulA"
                     /locus_tag="ACHVB7_001542"
     CDS             complement(21971..22480)
                     /gene="sulA"
                     /locus_tag="ACHVB7_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR1913562.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22699..23328
                     /gene="sxy"
                     /locus_tag="ACHVB7_001543"
     CDS             22699..23328
                     /gene="sxy"
                     /locus_tag="ACHVB7_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR1913563.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23291..25453)
                     /gene="yccS"
                     /locus_tag="ACHVB7_001544"
     CDS             complement(23291..25453)
                     /gene="yccS"
                     /locus_tag="ACHVB7_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR1913564.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25463..25909)
                     /locus_tag="ACHVB7_001545"
     CDS             complement(25463..25909)
                     /locus_tag="ACHVB7_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR1913565.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26032..28086
                     /gene="helD"
                     /locus_tag="ACHVB7_001546"
     CDS             26032..28086
                     /gene="helD"
                     /locus_tag="ACHVB7_001546"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR1913566.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISSQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28118..28576)
                     /gene="mgsA"
                     /locus_tag="ACHVB7_001547"
     CDS             complement(28118..28576)
                     /gene="mgsA"
                     /locus_tag="ACHVB7_001547"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR1913567.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28672..29334)
                     /locus_tag="ACHVB7_001548"
     CDS             complement(28672..29334)
                     /locus_tag="ACHVB7_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR1913568.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7552 a         6966 c         7741 g         7134 t
ORIGIN      
        1 gtacccctct agtgttctgg aggggatatt catatttttt aagagtgact atttatgaaa
       61 acttgcataa caaagggaat tgtgaccgta agtttaacgg caatattact cagctgctca
      121 tccgcatggg cggcaggtaa aggcgggatt ggacttgcag caacacgtct tgtttattca
      181 gaaggtgaag agcaaatttc actgggtgta cgtaacacca gtccggatgt tccttatctt
      241 attcagtcat gggtgatgac cccagataat aaaaaatcag cagattttat tattacacca
      301 ccgctatttg tgctgaatcc ggctaatgag aatctgttac gcattatgta cattggtgcg
      361 ccgttggcga aagacagaga aacccttttc ttcactagcg tacgggcagt cccttcaaca
      421 acgaagcggg aagagggaaa taccctgaag attgccacac aaagcgtcat caaacttttc
      481 tggcgaccaa aaggtttagc gtatccctta ggcgaggctc cggcgaaact gcgttgcact
      541 tcgtcagctg acatggttac ggtcagtaac ccaacacctt atttcattac cctgacagac
      601 ctgaaaatag ggggaaaggt agttaaaaat caaatgattt ccccctttga taaataccaa
      661 ttctctctgc caaagggggc caaaaatagc agcgtaacgt atcgaaccat caatgattac
      721 ggggcggaaa cgccacaact caactgtaaa tcgtaagccg tcttcagtta agagagcgag
      781 atgtatagaa ctcatcgaca acacagcctg ttaagctctg gtggagtgcc atcgtttatt
      841 ggtgggctgg tggtgtttgt gtcggcagcg ttcaatgcac aagctgaaac ctggttcgat
      901 cctgcttttt tcaaagatga tccctcaatg gtggccgatt tgtctcgttt cgaaaaagga
      961 caaaaaataa cgccaggggt ttatcgtgtc gatattgttc tgaatcagac aattgtagat
     1021 acgcgcaacg tcaattttgt tgagataacg ccagagaagg ggattgccgc ctgtttgacg
     1081 actgaaagcc tggatgcaat gggtgttaat actgatgcgt ttccggcttt taaacaactg
     1141 gacaaacaag cgtgtgtgcc attggcggag attattccgg atgccagcgt aacgtttaat
     1201 gtgaataaac tccgtctgga aatttcagta ccgcaaatcg ccatcaaaag taatgcccgt
     1261 ggttatgtcc cccctgaacg ttgggatgaa gggatcaacg cgctattact gggatattcg
     1321 tttagcgggg ctaacagtat tcatagcagc gcagacagtg attctggcga cagctatttt
     1381 ctgaatttaa acagtggcgt taatttaggc ccatggagat tgcgcaacaa ttcaacatgg
     1441 agtcgcagta gtggccaaac cgcagaatgg aagaatctca gcagctattt gcagcgggcg
     1501 gttattccac taaaaggcga actgaccgta ggtgatgatt atactgcagg cgattttttc
     1561 gatagtgtca gctttcgtgg tgtgcagctg gcgtcagatg acaacatgct gccagacagc
     1621 ctgaaagggt ttgcgcctgt ggtgcgtggt atcgccaaaa gcaatgccca gataacgatt
     1681 aagcaaaatg gttacaccat ttaccaaact tatgtatcgc ctggtgcttt tgaaattagt
     1741 gatctctatt ccacgtcgtc gagcggtgat ttgttagttg aaatcaaaga agcggacggc
     1801 agcgtcaata gctacagcgt accgttttcc agcgtgccat tactccagcg tcaggggcga
     1861 atcaaatacg cggtgacact ggcgaaatac agaaccaata gtaatgaaca gcaggagagc
     1921 aaatttgcct aggccacgtt gcagtggggc ggaccgtggg gaacgacatg gtatggtggt
     1981 ggacaatatg ctgaatatta ccgtgccgcc atgtttggtc tgggatttaa ccttggcgat
     2041 ttcggagcaa tttcgttcga tgcgacccag gcgaagagta cgctggcaga ccaaagcgaa
     2101 cataaaggtc agtcatatcg ttttctgtat gccaaaacgc tcaaccaatt gggcactaat
     2161 tttcaattga tgggctatcg ctattcgacg tcgggtttct acaccctttc cgacaccatg
     2221 tataaacata tggatggcta cgaatttaat gacggtgatg atgaagatac gccgatgtgg
     2281 tcgcgttatt acaatttgtt ttacaccaaa cgtggcaaac tgcaggtcaa tatctcccag
     2341 caattaggcg agtacggttc gttttattta agtggtagcc agcaaactta ctggcatacc
     2401 gatcaacagg atcggctatt acagtttggc tacaacacgc aaattaaaga tctctcgctg
     2461 gggggttcct ggaactacag taagtcccgt ggtcaacctg atgctgatca ggtgtttgca
     2521 ctaaattttt ccctgccgct caatctgttg ctccccagaa gtaatgatag ctataccagg
     2581 aaaaaaatta cgcctggatg acctctaaca ccagcatcga taacgaaggg cacattacac
     2641 aaaacctggg tttaacggag acactactcg atgacggtaa cctgagctac agcgtgcaac
     2701 agggatataa cagcgagggg aaaacggcta atggtagcgc cagcatggac tacaaagggg
     2761 cgtttgcaga tgcccgagtg ggctacaact acagcgataa cggcagtcaa caacaactga
     2821 actacgctct ttcaggcagt ttagttgccc attcacaggg cattaccctg gggcaatcgc
     2881 tgggggaaac taacgtgctg attgcagcac caggcgcaga gaatactcgt gtggcgaaca
     2941 gcaccgggct gaaaactgac tggcgcggat ataccgttgt cccctatgcc acttcttatc
     3001 gggaaaatcg aatcgcactt gatgcggcat cgttaaaacg taacgtggat cttgaaaatg
     3061 cagtagtcaa cgtggttccc accaaagggg cgttggttct ggcggagttc aatgcccatg
     3121 cgggagcaag ggtattaatg aaaacatcaa agcagggtat accgctgcgt tttggcgcga
     3181 tagcgacgct ggacggcgta cagactaata gcggcattat tgatgatgat ggctcgctct
     3241 atatgtccgg tttgcccgct cagggagcga taactgtacg ctggggcgaa gctcccgatc
     3301 aaatttgtca tatcagttac cagcttaccg aacaacaaat taactctgcg attacgcgaa
     3361 tggatgccat atgcagataa tctttggaga aaaatgcgtg ttattactac gactattttt
     3421 tgccgccgtc ttaatgctat ggtgcgctca aactgctgct tatagcgggc agtgtcatac
     3481 cactcagggg aatccgtata ttggcgtcaa ttttggcgtt aaaaccctgg aggaagaaga
     3541 aaatacggct ggagtagtta aagacaaatt ttatcagtgg aacgaatcga atgattatta
     3601 tgtttcctgt gattgcgata aagacaatgt cagaagtggc cgatgggcat tcgccgcgga
     3661 ttcaccgtta gtctatttag gcgacaactg gtacaaaatt aatgactatc ttgccgccaa
     3721 agttttattg caggttaaag gcagttctcc tactgcggtt cctttcgaaa acgtgggcac
     3781 aggggcagat acacgatggc atatttgcga tcccggcggt caacgtttag gtggccaggg
     3841 ggctagcggt aatagcggta gcttttccct gaaaatattg cagccgttcg ttggttcggt
     3901 cgtcattcct cctatggcgc tggcgcgatt atttgaatgc tacaacatac ccgcaggtga
     3961 ttcctgcacg actacaggta caccggtttt agtgtattac ctgtctggta cgatcaattc
     4021 acttggctca tgttccgtca atgccggaga aacaatcgag gtcgatctgg gcgacgcatt
     4081 tgcggctaac tttcgtgttg tagggcataa gcctcttggg gccagaacgg cagaacttgc
     4141 aattccagtc aggtgtaaca cgggaaacgc ggggttagtt aacgtcaacc tgagtctgac
     4201 ggcaaccaca gaccccagct atccccaggc gattaagacg tcacgtcctg gcgtgggcgt
     4261 ggtggtgacc gatagccaga acaacattat ttcccctgct ggtggaacat taccgctctc
     4321 tattcctgat gatgcagaca gtatcgcgcg aatgaatgtc tatccagtca gcacgacagg
     4381 tgtaccacca gaaaccgggc gatttgaagc cacggcaacg gtgagaataa attttgatta
     4441 acgcgtgaac gtatgaagaa agaaacgata tttcagtgcg ttatcttgtt ctttagcatt
     4501 cttaacatcc atgtcgggat ggctgggcct gaacaagtta gtatgcatat ttatgggaat
     4561 gtggtcgatc agggctgtga tgtcgccacc aaaagtgcat tacaaaatat tcatattggt
     4621 gattttaata tcagtgattt tcaggccgcg aataccgtaa gcactgctgc tgatttgaat
     4681 atcgatatca ccggttgtgc cgctagtatt actggcgcgg acgtcctttt tagcggcgag
     4741 gctgacaccc ttacgccgac actgctcaaa ctaactgaca caggcggaag cggtggtatg
     4801 gcaacgggga ttgccgtgca aattcttgat gcgcaaagtc agcaagaaat cccgctcaat
     4861 caggtccagc ctcttacgcc cttaaaagcc ggggataaca cactcaaata tcaacttcgt
     4921 tataagtcca caaaggcggg agcaacgggc ggtaatgcga cggcggttct ctattttgat
     4981 ctggtttacc agtgaaggag tgatgttgaa acggataatc tggattctgt tcttactggg
     5041 attaacgggg ggctgtgagc tatttgccca tgatggcacg gtcaacatta gcggatcgtt
     5101 tcgccgtaat acatgcgtgc tggcacagga tagcaagcaa atcaatgtgc agttaggcga
     5161 tgtctcgctg acacgttttt ttcatgggaa ttatggcccg gagaaatctt tcatcattaa
     5221 tttgcaggat tgcggaacag atgtcagcac ggttgacgtc accttttcag gaacccccga
     5281 cggagtgcag agcgaaatgc tgtccattga aagtggtacg gatgcagcca gcgggctggc
     5341 aattgcgatt ctggatgatg cgaagatatt gattccgctt aaccaggcga gtaaagacta
     5401 cagcctacat agcggtaagg tgccattaac tttttatgcg caattgcgac ctgtcaatag
     5461 cgatgtgcag tcgggtaaag tgaatgccag cgcgacattt gtacttcatt atgactaata
     5521 cgtggaacag attggcgctc ttgattttcg ccgttttatc gctgctggtg gcgggtgaat
     5581 tgcgggcagg agtggtggtc ggcggaacgc gatttatctt tccggcagac agagaatcga
     5641 tatctatttt actgactaat accagtcagg aatcctggct tattaacagt aaaatcaacc
     5701 gcccaacgcg ttgggccggg ggggaagcgt cgacagtgcc agcaccatta ctggccgctc
     5761 cgccacttat tctcctgaag cccggtacta caggcacgtt gcgcttgctg agaacggaaa
     5821 gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt aagcattgcc agcgtgccat
     5881 ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg ctcggtattt aaactgttct
     5941 ggcgacccga aggtttgccg ggcgacccgc tggaagctta tcaacaatta cgctggacac
     6001 ggaattccag ggtgtacaac tcactaaccc aacgccttac tacattaacc tgattcaggt
     6061 gagcgtaaat ggtaaagcgt taagtaatgc gggagttgtg ccgcctaaaa gccagcgtca
     6121 gacaagctgg tgtcaggcaa tcgcaccgtg tcatgtcgcc tggcgggcga taaatgatta
     6181 cggcgggttg agtgcaaaga aggagcaaaa tctgccctga aacaggttcg gaaaacgttt
     6241 gcgttttttt tgccgcaggt caattccctt ttggtccgaa ctcgcacata atacgccccc
     6301 ggtttgcaca ccgggaatcc aggagagttc atgtactacc ccttcgttcg taaagccctt
     6361 ttccagctcg atccagagcg cgctcatgag tttacttttc agcaattacg ccgtattaca
     6421 ggaacgccgt ttgaagcact ggtgcggcag aaagtgcctg cgaaacctgt taactgcatg
     6481 ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc ttgataaaga cggggagtgc
     6541 attgacgcgt taggcgcgat gggatttgga tcgatcgaga tcggtaccgt cacgccacgt
     6601 ccacagccag gtaatgacaa gccgcgtctc tttcgtctgg tagatgccga aggtttgatc
     6661 aaccgtatgg gctttaataa tcttggcgtt gataacctcg tagagaacgt aaaaaaggcc
     6721 cattatgacg gcgtcctggg tattaacatc ggcaaaaata aagatacgcc agtggagcag
     6781 ggcaaagatg actatctgat ttgtatggaa aaaatctatg cctatgcggg atatatcgcc
     6841 atcaatattt catcgccgaa taccccagga ttacgcacgc tgcaatatgg tgaagcgctg
     6901 gatgatctct taaccgcgat taaaaataag caaaatgatt tgcaagcgat gcaccataaa
     6961 tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg aagaagaatt gatccaggtt
     7021 gccgatagtt tagttcgcca taatattgat ggcgttattg caaccaatac cacactcgat
     7081 cgttctcttg ttcagggaat gaaaaattgc gatcaaaccg gtggcttaag tggtcgtccg
     7141 cttcagttaa aaagcaccga aattattcgc cgcttgtcac tggaattaaa cggtcgctta
     7201 ccgatcatcg gtgttggcgg catcgactcg gttatcgctg cgcgtgaaaa gattgctgcg
     7261 ggtgcctcac tggtgcaaat ttattctggt tttattttta aaggtccgcc gctgattaaa
     7321 gaaatcgtta cccatatcta attatttctt cgaattcctc tttataagat aatcagggct
     7381 ttattttcag ccctggttgt tttatattca tcgctgttgc ttatttagac attttgtact
     7441 tttattgttg aggttattaa gcgaagcgac aatggattgt gttgctgcgg ttttataggg
     7501 taggggagag gcagatgcga attaaaccag acgataactg gcgttggtat tacgatgaag
     7561 agcacgatcg aatgatgctc gatttagcca atggtatgct atttcgctca cgttttgcgc
     7621 gcaagatgtt gactccagat gccttttctc ccgcaggctt ttgcgttgac gacgccgcgc
     7681 tctatttctc ttttgaagaa aagtgccgcg attttaattt atctaaagaa caaaaagccg
     7741 aactggtgct gaatgcactg gtagcaattc gctatctcaa accgcaaatg ccgaaaagct
     7801 ggcattttgt ttcccatggt gaaatgtggg tgcccatgcc gggcgatgcg gcctgcgttt
     7861 ggttgagcga tactcacgag caggtcaatt tgctggtcgt tgaatctggc gaaaatgccg
     7921 cactatgcct gctggcacaa ccctgcgttg ttattgcggg tcgcgcgatg cagttgggcg
     7981 acgccattaa aatcatgaac gacaggctga aaccgcaggt taatgttgac agcttcagcc
     8041 tcgaacaggc agtctaacgc gccaacttaa gtgcagtctt cggtacacag ctacagcaaa
     8101 gaatggtgcc atcatcgccc attgctgatt ttttcagcgg catgacttcg ccttctaaaa
     8161 gctgaacacg gcaacttcca caaatgcccg cgcggcaaga ataagggata cgaattccct
     8221 gattttctaa ttgctccagc aacacctgtt ggttatttcc acgaaatgcc tgtccctgcc
     8281 agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt ggcggtatca tcagcggcag
     8341 ctgcgccgta aatttttgcc ggagccgttg ccagaatttc cacctcatcg ccaacgcgaa
     8401 tcacgccgct attacgggca attaaattct gaccaaaatc gacatcgccg ttatcctggg
     8461 cagtgcggaa agattgcaat gtttttaatg gttcgcctgc cggatgtttt tgcccttttt
     8521 ctgggctgac ggtggtgaaa atacagcggc tacaaggttt aaccacatca aacaccacat
     8581 caccaatgcg aatcactttc cagcgatctt cttcccaggc tgacgcgccg gaaaccacca
     8641 gattggggcg gaattgctcc atttttacac tggccggaca acgttgttgg agatcacgta
     8701 acgaggcttc gttagcaaga aggtaaggat agccatcagc aaatgacaga ggtacagtgt
     8761 tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca gcgtaattgc acttcgcggg
     8821 agaaaaatcc acttagccat ttgttgatcg cgtctggcgc aattcgcgcg gtaaaatgtg
     8881 tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc agcaaaacga acatatgcgc
     8941 tactgccatc tggtgcggtg agatgcaagc catcatgcac aggtgaagga gtaaaccgta
     9001 ccatctgggg aaactggcga gcggtaataa acgtaccgtc aggttccgtg atcataaaga
     9061 tgcgatcgaa ggccagacca ctgacatcag ccagagcatg tgtaagacca atgccgcgca
     9121 tcgatttaac aggatgaata aaaagccggg ttaatgtcgc cacggtgcgg tcctcaaatg
     9181 aaaataagcc ctcaacttta tgacatgacg cgcttattag ctataatgcg caacaatttt
     9241 cttagcgcct gtcccattag gctattttat ttgtcagttt ggccctgggt tgtgctcgaa
     9301 atcctcacgt acttgtgtac gctccggttt ctccgcgctg tccatgtcca gactgactgc
     9361 aacaattacg cctactgcgg taggctctta gagtaaaagt gacgatatga attctctgtt
     9421 tgccagtacg gcccgtgggc tggaagagct gttaaaaact gaactggaaa acctgggggc
     9481 cgttgaatgc caggtggttc agggtggggt ccatttcaag ggcgacacac ggcttgttta
     9541 ccagagcctg atgtggagcc gcctggcctc gcgtattatg ttgccgctgg gcgagtgtaa
     9601 ggtttacagc gatttagacc tctatctcgg tgttcaggcg atcaactgga cagagatgtt
     9661 taatcctggc gcgaccttcg ctgtccactt cagtggtttg aatgacacca tccgcaacag
     9721 tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat gctttcacgc ggaaaaatct
     9781 gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc gttaacgtct ggctgcataa
     9841 agaaaccgcc agtatcgctc ttgatctgag tggtgatggt ttacatctgc gtggctatcg
     9901 cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca gccgctattg tgatgcgaac
     9961 cggctggcag ccaggaacac cgctgctcga tccgatgtgt ggttccggta cgttgctgat
    10021 tgaagcggcg atgctagcga ccgatcgcgc accaggcttg caccgtgggc gttggggctt
    10081 tagcggctgg gtgcagcatg atgaagctat ctggcaggaa gtgaaagcgg aagcgcaaac
    10141 tcgtgcccgt aaaggcctgg ctgagtatag ctctcatttt tacggttcgg acagcgacgc
    10201 acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg gggattggtg aactgatcac
    10261 ctttgaggtg aaagatgtcg cgcaactgac caatccgctg ccgaaagggc cgtacggcac
    10321 agtgttgagc aacccgccat acggggaacg tctggacagc gaaccggcgc tgattgcgct
    10381 gcatagcctg ctgggtcgga tcatgaaaaa ccagtttggt ggctggaatc tctctttgtt
    10441 tagtgcctcg ccggatctgc taagctgctt gcagctgcgt gcagacaaac agtacaaggc
    10501 gaaaaacggc ccgctggact gcgtacagaa aaattaccac gttgccgaaa gcactccaga
    10561 cagcaaaccg gcgatggtag cggaagacta cgccaaccgc ctgcgtaaga acctcaaaaa
    10621 attcgagaag tgggctcgcc aggaaggaat tgaatgttac cgcctgtatg acgccgatct
    10681 accggaatat aacgttgccg ttgaccgtta tgccgactgg gtggtggtgc aggagtatgc
    10741 gccgccaaaa actattgatg ctcacaaagc gcgtcagcgt ctgttcgata ttatcgctgc
    10801 aaccatttcg gtactgggga ttgcgccaaa caaactggtg ctgaaaaccc gtgaacgcca
    10861 gaagggcaaa aaccagtacc agaaactggg cgagaagggc gagtttcttg aagttaccga
    10921 atataacgct cacttgtggg tgaacctgac ggattatctc gatactggtc tgttcctcga
    10981 tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa ggcaaagatt tcctcaacct
    11041 gttctcttat accggtagcg ccaccgtgca tgcgggatta ggcggcgcgc gcagcaccac
    11101 caccgtggat atgtcgcgta cttatctgga gtgggcagaa cgcaacctgc gtctgaatgg
    11161 cctgaccggg cgtgcgcatc gcctgattca ggccgattgc ctggcgtggc tgcgtgaggc
    11221 aaatgaacag tttgatctga tctttatcga tccgccaacc ttctctaact caaaacgaat
    11281 ggaagatgcg tttgatgttc agcgcgatca tatggcgctg atgaaagatt tgaaacgtct
    11341 gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa cgtggcttcc gtatggatct
    11401 cgacggcctg gcaaaactgg gactgaaagc acaagaaatt acgcaaaaaa cgctctccca
    11461 ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg attaccgcag cctgaaagga
    11521 atagtaatgt cattaatcag tatgcatggc gcatggctgt cgttcagcga cgcgccgctt
    11581 ctcgataacg cagaactgca tatcgaagat aacgaacgtg tttgtctggt gggtcgcaac
    11641 ggcgcgggca aatcgacgtt aatgaaaatc ctcaaccgtg aacaagggct ggatgacggt
    11701 cgcattattt acgagcaaga tttgattgta gctcgtctgc aacaggagcc gccgcgtaac
    11761 gttgagggta gcgtttatga tttcgttgcc gaaggcattg aagaacaagc ggaatatctg
    11821 aaacgctatc acgatatttc gcacctggtg atgaacgacc cgagcgagaa aaatctcaac
    11881 gaactggcga aggttcagga acagttggat caccacaacc tgtggcagct ggaaaaccgc
    11941 atcaacgaag tgctggcgca actggggtta gatcctaatg ttgcgctgtc gtcgctttcc
    12001 ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg tgagtaatcc gcgcgtgctg
    12061 ttgcttgatg aaccgacaaa ccacctggat attgaaacca tcgactggct ggaagggttc
    12121 cttaaaacct tcaatgggac gattattttc atctcccacg accgttcgtt tatccgcaat
    12181 atggcgacgc gcattgttga tctcgatcgc ggcaagctgg tgacctatcc agggaattac
    12241 gaccagtacc tgcttgaaaa agaagaagcc ctgcgcgtgg aagaactaca aaatgccgag
    12301 ttcgatcgca aactggcgca ggaagaggtg tggatccgcc aggggatcaa agcacgccgt
    12361 acccgtaatg aaggccgcgt acgcgccctg aaagcaatgc gtcgcgagcg tggtgaacgt
    12421 cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg ccagccgctc cggtaagatc
    12481 gttttcgaaa tggaagacgt ttgctaccag gttgacggta agcaactggt gaaagatttt
    12541 tcggcccagg ttctacgtgg cgacaaaatt gccctgattg gtccgaatgg gtgcggcaaa
    12601 accacgctgc tgaaactgat gctcgatcag cttcaagcgg acagcgggcg tattcacgtt
    12661 ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg cggaactgga tcccgataaa
    12721 acggtgatgg ataaccttgc cgaaggtaag caagaggtga tggttaacgg caagccacgc
    12781 cacgtattgg gctatttgca ggactttctg ttccatccga aacgggcgat gacgccggta
    12841 cgtgcgcttt ctggcggtga gcggaaccgc ttgctgctgg cgcgtttgtt cctcaaacca
    12901 agcaacttat tgattcttga cgaaccgacc aacgatcttg atgtcgaaac gctggaactg
    12961 ctggaagaac tgatcgacag ctatcagggc acggtattgc tggttagcca cgatcgtcag
    13021 tttgtcgata acaccgttac agaatgttgg atcttcgaag gcggcggtaa aattggtcgt
    13081 tatgtcggcg gttatcatga tgcccgtggt cagcaagagc agtatgtggc gctcaaacag
    13141 cctgcggtga aaaaaaccga agaagccgcc gcggcaaaag cagaaactgt aaaacgcagc
    13201 agtagcaaac taagctataa attgcagcgc gaactggagc agctaccgca attgctcgaa
    13261 gatctggagg cgaagctgga agccctacag acgcagatgg cggatgcttc cttcttcagt
    13321 cagccacatg agcagacaca aaaagtgctc gccgatatgg ctgctgcaga gcaggagctg
    13381 gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa aaaatggtgg ctgatcgcaa
    13441 aatagtcgat ttaaacagag acagctgaca acggtaaaat tgcctgatga actgggttta
    13501 tcaggcatga aaaccaacac tttgttagcc atctgtaaaa tagcgcatca ttaaggagta
    13561 ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt gctcgcagtg tgacatgctg
    13621 gtggcgttac cgcgccttga gcatggtcag aaagcggcat gtccccggtg tggcacaacg
    13681 ttaaccgtgg cgtgggatgc ccctcggcag cgtccgaccg cctatgcgtt ggctgcactg
    13741 ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata tgaacgttgc aggagttacc
    13801 agtgaaatta cattactgga aattcccggc gtgctttttt ctgaggacta cgccagcctc
    13861 ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt tttgtctgat aaccattctg
    13921 ttactggtga atcgcgcgga attaccggtc cgtttaaaag agcaactggc acgggtgctt
    13981 tttcaactca aaacctgggg aatggcggag attttcctcg ctggtgtgct ggtcagtttc
    14041 gttaaactga tggcttacgg cagcattggg gtaggcagca gctttctccc ctggtgttta
    14101 ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc gtcgctggtt atgggacgac
    14161 atcgccccga tgccagaact gcgccagccg ctaaaaccag gcgtcacggg gatacgtcag
    14221 gggctgcgtt cgtgctcctg ttgtacggca atccttcctg ctgatgaacc cgtgtgcccg
    14281 cgttgtagta ccaaagggta cgttcggcgt agaaacagcc agcagtggac actcgcgctg
    14341 cttgtaacgt ccatcatgct gtatcttccg gctaatattt tgcccatcat ggtgacggat
    14401 ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg tcattctgtt atggagcgaa
    14461 ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta ttatggtgcc aacgttaaag
    14521 atgatcgcca tcgcgtggct gtgttgggat gccaaagggc atggcaagcg cgacagtgaa
    14581 agaatgcatt tgatttatga agttgttgag tttgtaggcc gctggtcgat gattgacgtt
    14641 ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag gtttaatgag tatttatccg
    14701 gcaatgggtg cattaatgtt tgctttagtc gtcataatga caatgttttc tgctatgacg
    14761 tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag agcatgagga gtcctgacag
    14821 tatggaatct aataatgggg aagccaaaat ccagaaagtg aagaactggt ctcccgtgtg
    14881 gatatttcct atcgtcacgg cgctcattgg ggcctgggtt cttttttatc attacagcca
    14941 tcagggactg gaagtgaccc tgatcaccgc gaatgcggaa ggaattgaag gtggcaaaac
    15001 caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa agcgccacac tggctgatga
    15061 tttgacgcac gttgaaatca aagtgcggct gaattctggt atggaaaaat tgctgcataa
    15121 agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc gaagggatta gcggcctggg
    15181 aacgctgctg tctggagttt atatcgaact gcagccaggc gcgaaaggca gcaaaatgga
    15241 taaatacgat ttgctggact cgccaccgtt ggccccgcct gatgcgaaag gtatccgtgt
    15301 gattctcgat agcaaaaaag ccgggcagct ctcgccagga gatccggtgc tgttccgtgg
    15361 ctatcgggta ggttcggttg aaaccagcac cttcgatacg caaaaacgca atatcagtta
    15421 tcaactgttc atcaatgcac cttatgaccg actggtgacc agcaatgttc gcttctggaa
    15481 agatagtggc attgcggttg atctgacgtc agcggggatg cgtgtggaga tgggctcatt
    15541 gacaacgctg cttagtggcg gtgttagctt tgatgtgccg gaaggtctgg atttagggca
    15601 gccagtggca ccgaaaacag ctttcgtttt gtatgatgat cagaagagca ttcaggattc
    15661 gttgtacacc gatcacattg attatctgat gttctttaaa gattcggtac gcggtctgca
    15721 accgggagct ccggtagaat tccggggtat tcgcctgggt accgtaagca aagtgccatt
    15781 ctttgcgccg aatatgcgtc agacatttaa cgatgattac cgtattccgg tactgattcg
    15841 tatcgagcca gagcggctga aaatgcagct tggcgaaaat gcggatgttg ttgagcacct
    15901 tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa accggaaacc tggtcactgg
    15961 tgcactgtat gttgatcttg atttctatcc aaatacgcct gcaataaccg gtattcgtga
    16021 atttaatggt tatcagatta tcccgactgt tagcggcggc ctggcgcaaa tccagcaacg
    16081 actgatggaa gcgttggata agatcaacaa actgccattg aatccgatga ttgaacaggc
    16141 aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac ctgcaaacga cgctggatag
    16201 catgaacaag atcctcgcca gccagtcgat gcagcagttg ccgacggata tgcagtcaac
    16261 gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct ggctccgcag cctacaacaa
    16321 gatggtggcg gatatgcagc gccttgatca ggtgttgcga gaactgcaac cggtgctgaa
    16381 aacgctcaat gagaagagta acgcgctggt atttgaagcg aaggacaaaa aagatccaga
    16441 gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga ttgcagcact gtggctggcc
    16501 ggatgcagct ccggcgaaat taataaaaac tattaccagt tacctgtggt gcagagcggt
    16561 acacaaagta ccgccagcca gggcaatcgt ctgttatggg tagagcaggt cactgttcct
    16621 gactatctgg cggggaatgg tgtggtttat caaaccagtg atgtgaagta tgtgattgcc
    16681 aacaacaact tgtgggccag cccgttggat caacaattgc gcaacaccct ggttgccaac
    16741 ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc ctctgggaag cgcccaggac
    16801 acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg atggcaaggt cattgtcagt
    16861 ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac gtccgttccg tctggaagga
    16921 gtgcaaactc aggatggtta cgatgagatg gttaaagtac tggccagtgt ctggagtcag
    16981 gaagccgctt ctattgcgca agagataaag cgtctacctt aattataaag atttgtaaat
    17041 ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt ttctaacgtg cggaaaaagt
    17101 tgttcctctt tacatttttt gtacaaccga catacccgtg tagctcacaa atatgacagt
    17161 ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg tattgcttaa ctgtgattgc
    17221 acatttagta atcactgttt tcttttccac cagaaaccag tatgagggaa acgaggcatg
    17281 aagagacaaa aacgagatcg cctggaacgg gcacatcaac gtggttatca ggccggcatc
    17341 gccggacgct caaaagaaat gtgtccctat cagacgctga atcaaaggtc acaatggctg
    17401 ggaggctggc gagaagccat ggcggacagg gtagtaatgg cctgattctg tctctttaaa
    17461 aagaaacctc cgcattgcgg aggtttcgcc ttttgatact ctgtctgatt ataatcagaa
    17521 ggcagaagta tcctggaaca gaccgacttt caggtcgctg gcggtataga tcaaacgacc
    17581 atcaaccagc acttctccat ccgccaggcc cataatcaga cgacggttaa caatgcgttt
    17641 aaagtgaata cggtaggtca cttttttcgc tgtcggcagt acctgaccag tgaatttcac
    17701 ttcgccaacg cccagtgcgc ggcctttacc ttcgccgccc agccagccga ggtagaaccc
    17761 taccagctgc cacattgcgt ccaggcccag gcatcccggc ataaccggat cgccaataaa
    17821 gtggcatccg aagaaccaca gatccggatt gatatccagt tctgcttcaa catacccttt
    17881 gtcgaagtta ccacccgttt cggtcatttt gaccacacgg tccatcatca gcatattcgg
    17941 tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg cgaccagagg caagaaggtc
    18001 ttcttttgta taggattcgc gtttatctac catgttctct gtaagcctta ttttattgaa
    18061 gcacgcagga tagctaacac gtgtacgctg aacaagtccg atcagttcgg aataaaccag
    18121 ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt cctgttgcga tgcttgcgcg
    18181 atacgttcct ggatggtttg catcagcgtc gtttggcctt cgccatccca caccagattt
    18241 aataataacg gcagtgcgtc agtcacatcg tctactgccc agatggtgaa tttgccttct
    18301 tctaccgctt tcactagttc actgtgaaga cttaaatggc gaacgttagc tgtggggata
    18361 atgacacctt gtttcccggt taactcacgt tgctggcaaa tagcaaagaa gccttcgatt
    18421 ttctcattta aaccaccgac cggctgggcg cgaccgaact gatcgactga acctgtgata
    18481 gcgatactct gattcaccgg cacatcggcg agggcgctta tcagggcgca gagttcagcc
    18541 atcgaggcac tatctccatc aacttcactg tatgactgct caaatgtcag cgatgctgag
    18601 aaggggatct gttgctcaag ctgtagttcc gacatcagga acgcttgcat gatcatcatc
    18661 cctttcgcat ggatattgcc gccaagctcc gctttgcgtt cgatgtcggt gaattcacca
    18721 tcgccaatat gcacaacgca gctaatgcga gaaggttcgc caaaagcgcg tggatgaccc
    18781 ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc gttcgccttc ggtttcaatc
    18841 aggatttgct cctgaaggat ctcatcctgc atacgttcag cgaggaaacc ttcgcgccat
    18901 tcacgctgct gcagcattaa gtttagctgc tcgccggaga aggtgtcgcc atcacacagg
    18961 gaggcgacct ctttacactg gcggaggatc cactgcgggc taagcggaag tgtttcttgt
    19021 tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc aggcatccgc tcccggtgca
    19081 ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac accactgggt tactgactcc
    19141 gcatcgacaa tctgcagagt atcttcaaat tcgctataaa tagcctgctc tgaaagctct
    19201 ggctccatct cctggaaatc agccaatgat tcgcgttcgc ctaccagaat gactttcagc
    19261 ttcaatggca tcgaaggcac agagacgggg agagggcgcg actcatcaaa cgcaacccag
    19321 tcaaaacgct cgcggttaac gatatttttc agccgcatcc acagcagagg ttgcgccagc
    19381 agtgtacgca aagagatgat gagaataccg ccatttgcct gatgcaccag accaggctgc
    19441 agggtaatgt cgccattaaa ctggcgcagg cagccaaaga gttgctccgc ttctacccag
    19501 tcggcagcga caacttgcgt taaagtcgca aaattatcat ctgcactcac tgcgtgacgt
    19561 aagcggatgg agtggccgga aacctcatag tgaccgccca ccagttggcc tgcatcgctt
    19621 tgtagcgtac gcgcggcatt ggcaataaga ttcagatact cagactcttc cggggccttc
    19681 gccagcataa aagaggagga tgctcgcgta tgcagcaact gctccagcgc aaattgcagc
    19741 cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa tcaaatgtgg ctgagcaaat
    19801 atttcctgat agctatcggt atcaggaacc aggtcacgcc atgcaagttt cgtaatggtc
    19861 aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta taccgtaacc ggcactacag
    19921 cacacgtaga aagcaccgac aatactccag gcatgggcgt taaagctcac aggatggaga
    19981 ttcttttctt cactggccta aaaagctgat attctgtaaa gagttacacg gtaacattga
    20041 gatcgctatg aaatatcaac aacttgaaaa tcttgaaagc ggttggaaat ggaagtatct
    20101 ggtgaagaag catcgcgaag gggagttaat cacccgttac atagaagcca gtgccgccca
    20161 ggaagccgtc gatgtgttgc tctcgctgga aaatgaaccc gtgctggtaa atggctggat
    20221 tgacaagcat atgaatccgg aactggtcaa tcgcatgaag caaaccattc gggcaagacg
    20281 aaagcgccat tttaatgcgg aacaccagca tacgcgcaaa aaatcgatcg atctggaatt
    20341 tattgtctgg caacgtctgg ctggtcttgc gcagcgtcgc ggtaaaacgc tttctgaaac
    20401 gattgttcag ctgattgaag atgcggaaaa caaagagaaa tacgcgaata aaatgtcttc
    20461 tctgaagcag gatctgcagg cattgctggg taaggaataa ctgacgaaag tcagttcaat
    20521 ttactaaagg caaaaaaaac cccgcagcag cggggttttt ctaccagacg agaacttaag
    20581 cctgcggctg agttacaacg tctttgatac ctttaacttc gatctctacg cgacgatccg
    20641 gagccaggca gtcgatcagt gcagcacgct gtttcacgtt gtcacaggtg ttgccagtaa
    20701 ccgggttgga ttcgcccata ccacgtgcgg agatcttgtc tgccgggata cctttggaga
    20761 tcaggtaatc aacaacagac tgagcacggc gctcggacag accctggttg taagcgtcag
    20821 aaccgatgcg gtcggtgtaa cccagaacaa ctacggaacc gtctttcgga tccaggttgc
    20881 tcagctggct gtacagctga tccagagcag cctgaccttc cggtttcagg gttgctttgt
    20941 tgaagttgaa cagaacgtca gacttcagag tgaagtgctt ggtctgtact tccggtgccg
    21001 gagctggagc cggagcaact actggagctg cttcgccctg accgaaacgg taggaaaccc
    21061 ccaggctcag catgccgttg tccggacgag tgccgatggt gtgtgcgtca ccgatgttgt
    21121 tggtccactg gtattccaga cgggtagcga tttcaggagt gatcgcgtac tcaacaccgc
    21181 cagcgaagac cggagaaacg ccggtgtcgt ggtttttacc ataaacgttg gatttagtgt
    21241 ctgcacgcca taccatacca cccagacgag tgtagatgtc caggtcgtca gtgattgggt
    21301 aacccagttt agcggtcagt tgaacgccct gagctttgta tgcaccgttt tcaacgctgc
    21361 ctttgtacgg catacgacct aaccagtcgt aacccatttc aaagccaaca tacgggttaa
    21421 cctggtaacc accaaaagca ccagcgccca gttggttttc atgggtcggg ccattgttgt
    21481 tgatgaaacc agtgtcatgg tactgggacc agcccagttt agcaccagtg taccaggtgt
    21541 tatctttcgg agcggcctgc gctacggtag cgaaaccagc cagtgccact gcaatcgcga
    21601 tagctgtctt tttcattttt tgcgcctcgt tatcatccaa aatacgccat gaatatctcc
    21661 aacgagataa cacggttaaa tccttcaccg ggggatctgc tcaattaact ctaccgatat
    21721 cttcggctta tgccgagcac ccctggcgat gtaaagtcta caacgtagtt gaaaacttac
    21781 aagtgtgaac tccgtcaggc atatgaaaaa aaagtcttgt ataaggtatg tttaatcttt
    21841 tttgtcagcg acaatttaca gaagaaaatc gcggaaaccg cttcagacaa gcctccgcaa
    21901 ggaaaattag tcacgactga aagcattggc tgggcgacaa aaaaagttcc aggattaatc
    21961 ctaaatttac ttaatgatac aaattagagt gaatttttag cccggaaagt tgtctcgtgg
    22021 cgtgagagga tgcgcttacc ggacgcataa taaaccccat agcgttacct tcatttgccg
    22081 catcaacaag ttcagcatgc tcttcttcag tcaaatcatc tgccaaccaa ccgatcacca
    22141 cactgtaatt gcccgtgcgt aaagcgcgaa ccattgactc cacagtgtgg caaggggaga
    22201 gctggctaat ctgcattact ttcgttaagg gtagcccaga tgcctgaacc cattcccgac
    22261 tcagtttttg ttgcggtgtt aaccagagtt gccagcgcga ttgctgaccg agttgctgta
    22321 acaatggcaa cagtagaagt tgcgtcatca tgggctgatc ttcgcgatag acaacttcac
    22381 tgataagccc ggctgtagtg ttttccgtag agacacgcgc aattttactt gctgcggatg
    22441 agaacgacga agaacgatgt gcatagcctg aagtgtacat aatcaatcca gcccctatga
    22501 gttactgtat ggatgtacag tacatccagt gacaacaaag atcaacccta ttttcggaaa
    22561 gagcctcgca aattttgtcg ttggtgacgg gaaaacataa attaatcttg ccccttaaga
    22621 ataagttgcc tattttcgta gttaacggat ccgttaatgt gaatcattct tttatgttat
    22681 gattttaaaa ggaattttat gaaaagcctc tcctataagc ggatctataa atcacaagaa
    22741 tacctggcaa cgttgggcac aattgaatac cgatcattgt ttggcagtta cagcctgacc
    22801 gttgacgaca cggtgtttgc gatggtttct gatggtgagt tgtatcttcg ggcttgtgag
    22861 caaagtgcac agtactgtgt aaaacatccg cctgtctggc tgacatataa aaagtgtggc
    22921 cgatccgtta ccctcaatta ctatcgggtt gatgaaagtc tatggcgaaa tcaactgaag
    22981 ctggtgcgtc tgtcgaaata ttctctcgat gcagcgctga aagagaaaag cacgcgcaat
    23041 acccgggaaa gactgaaaga tttgcccaat atgtcttttc atctggaagc gattctcggg
    23101 gaggtgggga ttaaggatgt acgggcgtta cgtatacttg gggcaaaaat gtgttggttg
    23161 cgactgcggc agcaaaacag tctggtgaca gaaaagattc tgtttatgct tgaaggtgcc
    23221 attatcggca ttcatgaagc tgcgctcccg gtggcacgcc gccaggagct tgcagaatgg
    23281 gctgactctc ttacgccgaa acaggagttt cctgcggaac ttgagtaatc tggcgttgca
    23341 gacgaccaat ctcaggcagt aatgcaatca ataatccaac ttgttgtacg accagaggtt
    23401 ctttgctgtc tgcccgtggt tcaagttgct gcatccgctg tttcaggcta gctaatgcct
    23461 cattgacgcg ttcttcatca gcaggttgat gatgtaacgc gtcatcaaca tagcaaactg
    23521 cgtcatcaag aaacgccaga atttcaggat tagttaactg ctcccggtga gcaccgaggg
    23581 ctgagatata gctggtaaac gtatggttaa ggcacagcaa ccgaaacgcg gcttcgcgaa
    23641 tttgcggggt aacgttcggc tcgctggaca tatttgatac caccgacgcc agctcagcat
    23701 cacggttgtg tgcatcgcgg cgggcaatac gatacgccag acggttatca cgcccctgat
    23761 ggtattgctc cagtatggca tcgagatacc gacagttggc ctctgtggcg cgttcgagca
    23821 tgcgcggcag attgcgaaac tgccagtcag gccagatgta gctcactgcc gcccacgcaa
    23881 tggcacaacc aatcagcgta tcgattacgc gaggtaacgc tacttcaaaa ccttcaccca
    23941 gtaagttaaa acacagtagc accaaaagtg tgatgaacat cgttgcatga gcgtattgca
    24001 cgttacggaa ggcaaaaaag agcacgccgg taataaccag cagcaccagc tgcccttcca
    24061 gtgatggcac aaaccacagc acaggaatgc caatggcgat acctaccagc gtaccaataa
    24121 tccttaactt caggcggtgg cgcgtggcgt tatagtttgg ctggcagaca aacaaacttg
    24181 tcagcaagat ccaatacccg tgatgcattc cggttatctg aatgatggcg tagccgaagc
    24241 acaataccag cgacattctt accgcatgac ggaagagggc ggattccggc gtgaagtgac
    24301 ggctaagacg cagccagata tcactcaacc cgtgcgggct gtcatcagcg agctcatttt
    24361 cgtcattatt atggggtagt gcctgggcct gttctgattc aattgttgcc agttgggcat
    24421 caatggcgcg taaattgttc agcaaaaatc ccagtgtttt gagtaaatcg gcgggtgcgc
    24481 cgttatcgcg catccgctcc agcgcagcat caatatgcgt aaaagcgcgc tcaaaatgcg
    24541 gatcatgttg ataaggctga cgcaacaaaa tacagcgtga cagttgctgg cacgcctggc
    24601 cctgcatcga catcagccgc tgaaaacgga acagcacgtc gctgtggcga aaatgttcac
    24661 gcaatgtttg atactgaata tgagaagagc tggcacgctc gtgaatatcc tgtgcgacaa
    24721 agtaataatg cagcgtgcga cgcgttcccc gttgaccacg atcgccacgt aagcgggtca
    24781 gcagcgagag tttcgtctga ttcaatgtcg ccatcagcag accgttggcg agagccaaat
    24841 cgtacagcgg tgcctggctt tcatcttcaa tatcaggatc aaacatgcgc gacttgagct
    24901 caagataacg cgccagttgt tcatagcaac gcgccaggtt gtcctgcagc gggcggaccg
    24961 ggaacagcag atgaccaata agtgtcagga cgttgtacca gacggcaccg gccagcagat
    25021 acatcggctg ctgataccag tgctcataca gtgatgttcc caacatagtg taaatggcga
    25081 tcagcaatgc accgaaggca attgttgcat agcgttgacc cagaccgccg agcaaaatga
    25141 agccgctggt agagagcgtt aagccaatcg caaatagcca gggccaggga aacagcaatt
    25201 ctactgaggc cgaggcgata aaaaagcaga acagcgtaat gatgaggtta cgcaaacgtc
    25261 ccgccagtcg gtcatcgaga tcggtcagcg ccgctgccac catccccagc gttagcggaa
    25321 tcgtcagttt tacatcaccc agccaccacg gaaacgctgt ggttccacaa agcgcaataa
    25381 aaatacgcgc gtaatacagc caggcgctgt tccaggtata gcgtttgagc aaaggactta
    25441 gcataaaggc catattagtg gattattcaa aacgacgacg tgcattggct tcacgcgcag
    25501 cttgcgctgt ttctaccgat accacgcgac gaccaaccgg ccatagtgca atagcggcaa
    25561 ttttaaagtt cgcaatgccg acaggaatgc caatgattga aatacattgt gcgatgcccg
    25621 ttgcaatgtg catcaggcat aaccaccagc caaagaaaat cagccagaaa atattcaata
    25681 ccgtaccgcc agtattcagc agcacatttt tgccagccgg gttcagttca tcgacatgaa
    25741 tagcttcatt gccataaggc accagagata gtttagtgat ctcccagcag gatcgtgtca
    25801 gcggtaaggt aaaaatcagc acaatactga ccagagtcgc caacagccag cccagagtgg
    25861 tggcaaatcc gccaagcaca aagttcagaa tgttcaaaac ggtacgcata aaacctcgct
    25921 ttactgtggt tttcagtaat gggcggcaat tgtaacgttt ttttgggctg gagcacgttt
    25981 tctctgacgg ttacactgat aagaaataat ttcgtgtgga tctacagagt catggaactg
    26041 aaagcgacaa cgcttggaaa acgtctggca cagcaccctt acgatcgggc ggtgatcctc
    26101 aatgccggga ttaaagtctc cggcgatcgc cacgaatacc ttattccttt caatcaatta
    26161 ctggcgattc actgtaagcg cggtctggta tggggcgagc tggaatttgt actgccggac
    26221 gaaaaagtgg tgcgtctgca cggcaccgaa tggggcgaga cgcagcgttt ttaccatcat
    26281 cttgatgctc actggcggcg gtggagtggc gagatgagcg aaattgcgtc tggtgtttta
    26341 cgccagcaac tggatttgat tgccacgcgc actggagaaa ataaatggct gacgcgtgag
    26401 caaacctctg gcgttcagca acaaatccgc caggctttgt cggcgttgcc gttgccggtt
    26461 aaccgactgg aagaattcga taactgccgt gaggcgtggc gtaaatgtca ggcctggttg
    26521 aaagatattg aaagcgctcg gttgcagcat aaccaggcgt ataccgaagc catgcttacc
    26581 gagtatgcgg attttttccg ccaggtcgag tcttcaccgc tgaatccggc gcaggcccgg
    26641 gcagtcgtta atggcgagca ttctctgtta gtgctggcag gtgcaggaag cggaaaaacg
    26701 tcggtgctgg tggcccgtgc aggctggttg ctggcgcgtg gtgaagcgtc ccctgagcaa
    26761 attttattgc tggcgtttgg tcgcaaagcc gctgaagaga tggacgagcg gattcgcgaa
    26821 cggctacata ccgaagacat taccgcacgc acgtttcatg cgctggcgct gcatattatt
    26881 cagcagggca gcaaaaaagt tccgatagtc agcaaactgg aaaatgatac cgctgcccgt
    26941 catgaactct ttattgctga gtggcgcaag caatgcagcg aaaagaaagc gcaggcgaag
    27001 ggctggcggc aatggctgac ggaagaaatg cagtggtcag tgccagaagg taacttctgg
    27061 gatgatgaaa aattacagcg tcgccttgcc tctcgcctcg atcgctgggt aagtctgatg
    27121 cggatgcacg gtggtgcaca ggcagaaatg attgccagtg cacccgaaga gattcgcgat
    27181 ctgttcagta aacgtatcaa gttgatggcc ccgttattaa aagcctggaa aggtgcgctg
    27241 aaggcagaaa acgctgtcga tttttcgggc cttattcatc aggcgattgt gattctggag
    27301 aaaggtcgct ttatcagccc gtggaagcat attctggttg atgaatttca ggatatctcg
    27361 tcgcagcggg cagcgttgtt agcggcatta cgcaagcaaa acagtcagac gacgttgttc
    27421 gctgttggtg atgactggca ggcgatttac cgattcagcg gtgcgcaaat gtcgctcacc
    27481 accgctttcc atgaaaactt tggtgaaggc gaacgttgtg atttagacac gacttaccgt
    27541 tttaacagtc gtatcggtaa ggtggcaaac cggtttattc agcagaaccc aggccagctg
    27601 aaaaagccgc taaacagctt aaccaatgga gacaaaaaag ccgtcacgtt attggatgag
    27661 agtcaacttg acgctttgct ggataagctc tctggttatg ccaaaccgga agagcgcatt
    27721 ctgatcctgg cgcgttacca tcacatgagg cctgccagcc tggaaaaagc ggcaacacgc
    27781 tggccgaagt tgcaaatcga ctttatgacc attcatgcca gcaaagggca acaggcggat
    27841 tacgtcatca tcgttggctt gcaggaggga agtgatggtt ttccggctgc ggcgcgggag
    27901 tcgattatgg aagaggcgct actgccaccg gttgaggatt tcccggacgc tgaagaacgg
    27961 cggttaatgt acgtggcgct gacccgggca cgccatcggg tatgggcact gtttaacaaa
    28021 gagaatccct ctccctttgt ggaaatactg aaaaatctgg atgtgccggt ggcgagaaaa
    28081 ccgtaagaaa caggtggcgt ttgccacctg tgcaatatta cttcagacgg tccgcgagat
    28141 aacgctgata atcggggatt agaatatcga ccgcgtcgtt gaaatgcggc gactggatta
    28201 tgaagtctgc cgttgccacg ttggtggcga ccggaatgtt ccataccgtc gccagacgca
    28261 gcaaggcttt cacgtcagga tcgtgcggca cggcatttag tggatcccag aagaaaatca
    28321 atacatcaat tttcccttct gagatcaatg cgccaacctg ctggtcaccc cccattgggc
    28381 cactcaacat cgcgttgacg ttcatgccgg tcgcgcggga aattaagtta ccggtagtgc
    28441 ctgttgcata cagtacgtgt tgttccagta acggttgatg ccgttccacc cagctcatca
    28501 gcatttgttt gcagtgatcg tgtgccacca gcgcaatatg tttccgcgca ggtaaagtgc
    28561 gagtcgtcag ttccataatg tacatccgta gttaactttc ctacagatta ctgtaagcac
    28621 ttatcgctgc aagataaaga ccgaaaaagc ctgcgcacag gcacaaaaat ctcaggaaga
    28681 tggttgtttt tccgcccact gcaggaaagt atttcgcgtt tgtgggtcag ccagtttaaa
    28741 ccaatacttc agccgttgtt ctgtgagcac ctgagactgc ggtggaatag catccagctc
    28801 ggaaacaccc gaaagcagcg tactgtcatc tgccatcatc gtggcaaatt gcggtagtga
    28861 agcgcgtttc gcggatttgt tatagcgttc aacctcgacc tcataatcaa tcgtttttgc
    28921 cgttgaggta atggcgagaa tatccagctt taccggaatc ggcgtcgcat cgccatccaa
    28981 caattcaagg cgcggcgcgg catcaaaatg gttagcttcc cgctcattct ccaggcgagg
    29041 caggcgaaaa ttgacctggt tgatgagctg ggtattaaaa ctgaccacca gcggtgggga
    29101 gatatacagc cgttcttcac tattggaaag atgaatcgtc ttctcaacgc gaaacactaa
    29161 ctgatgcggt ccattatcca gttcaatgct gtccgcaccg cgcagcagag agctggaaac
    29221 cttcttgccg tcgagtacca gaagatcgac atcggttgaa agccgcagcg tggtggcaaa
    29281 aacggatacc ggcaaacata atgcaattaa ggtggtcacg atgccggttt tcataggtag
    29341 ctcctgtcaa aagaccgccc gaataataat gtgtcaaaat ttttctttaa tca
//
LOCUS       DAZPJR010000038        28797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPJR010000038 DAZPJR010000000
VERSION     DAZPJR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.039"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHVB7_001549"
     CDS             complement(<1..215)
                     /locus_tag="ACHVB7_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1913569.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHVB7_001550"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHVB7_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR1913570.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHVB7_001551"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHVB7_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR1913571.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHVB7_001552"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHVB7_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR1913572.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHVB7_001553"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHVB7_001553"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR1913573.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHVB7_001554"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHVB7_001554"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR1913574.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHVB7_001555"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHVB7_001555"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR1913575.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHVB7_001556"
     CDS             complement(4604..5326)
                     /locus_tag="ACHVB7_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1913576.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHVB7_001557"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHVB7_001557"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1913577.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHVB7_001558"
     CDS             6526..6744
                     /locus_tag="ACHVB7_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR1913578.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHVB7_001559"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHVB7_001559"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1913579.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHVB7_001560"
     CDS             complement(7478..7678)
                     /locus_tag="ACHVB7_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR1913580.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHVB7_001561"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHVB7_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR1913581.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHVB7_001562"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHVB7_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR1913582.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHVB7_001563"
     CDS             10175..12088
                     /locus_tag="ACHVB7_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1913583.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHVB7_001564"
     CDS             12088..13110
                     /locus_tag="ACHVB7_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1913584.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHVB7_001565"
     CDS             13104..13322
                     /locus_tag="ACHVB7_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR1913585.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHVB7_001566"
     CDS             13376..14245
                     /locus_tag="ACHVB7_001566"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR1913586.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHVB7_001567"
     CDS             complement(14300..14704)
                     /locus_tag="ACHVB7_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR1913587.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHVB7_001568"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHVB7_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR1913588.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="ACHVB7_001569"
     CDS             15689..17779
                     /locus_tag="ACHVB7_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR1913589.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHVB7_001570"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHVB7_001570"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR1913590.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHVB7_001571"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHVB7_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR1913591.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHVB7_001572"
     CDS             complement(19747..20349)
                     /locus_tag="ACHVB7_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR1913592.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHVB7_001573"
     CDS             complement(20339..20506)
                     /locus_tag="ACHVB7_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR1913593.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHVB7_001574"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHVB7_001574"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR1913594.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHVB7_001575"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHVB7_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR1913595.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHVB7_001576"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHVB7_001576"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR1913596.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHVB7_001577"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHVB7_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR1913597.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHVB7_001578"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHVB7_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR1913598.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHVB7_001579"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHVB7_001579"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR1913599.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="ACHVB7_001580"
     CDS             28334..28501
                     /locus_tag="ACHVB7_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR1913600.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6995 a         7710 c         7219 g         6873 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctat
//
LOCUS       DAZPJR010000039        28678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPJR010000039 DAZPJR010000000
VERSION     DAZPJR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.040"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1046)
                     /locus_tag="ACHVB7_001581"
     CDS             complement(<1..1046)
                     /locus_tag="ACHVB7_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1913601.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSILPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="ACHVB7_001582"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="ACHVB7_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="ACHVB7_001583"
     CDS             complement(2433..3281)
                     /locus_tag="ACHVB7_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR1913602.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="ACHVB7_001584"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="ACHVB7_001584"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR1913603.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4183..4686
                     /gene="tadA"
                     /locus_tag="ACHVB7_001585"
     CDS             4183..4686
                     /gene="tadA"
                     /locus_tag="ACHVB7_001585"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR1913604.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="ACHVB7_001586"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="ACHVB7_001586"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR1913605.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="ACHVB7_001587"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="ACHVB7_001587"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR1913606.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="ACHVB7_001588"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="ACHVB7_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR1913607.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="ACHVB7_001589"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="ACHVB7_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR1913608.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="ACHVB7_001590"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="ACHVB7_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR1913609.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="ACHVB7_001591"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="ACHVB7_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR1913610.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="ACHVB7_001592"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="ACHVB7_001592"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR1913611.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="ACHVB7_001593"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="ACHVB7_001593"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR1913612.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="ACHVB7_001594"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="ACHVB7_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="ACHVB7_001595"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="ACHVB7_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="ACHVB7_001596"
     CDS             22669..23652
                     /locus_tag="ACHVB7_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR1913613.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="ACHVB7_001597"
     CDS             23675..25186
                     /locus_tag="ACHVB7_001597"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR1913614.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="ACHVB7_001598"
     CDS             25211..26209
                     /locus_tag="ACHVB7_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1913615.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="ACHVB7_001599"
     CDS             26242..27336
                     /locus_tag="ACHVB7_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1913616.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="ACHVB7_001600"
     CDS             27348..28220
                     /locus_tag="ACHVB7_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1913617.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28678)
                     /locus_tag="ACHVB7_001601"
     CDS             complement(28268..>28678)
                     /locus_tag="ACHVB7_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1913618.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6818 a         7093 c         7790 g         6977 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg caagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtatggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagacg ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaaactct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatgt ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacg
//
LOCUS       DAZPJR010000040        28204 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPJR010000040 DAZPJR010000000
VERSION     DAZPJR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.041"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHVB7_001602"
     CDS             <1..468
                     /locus_tag="ACHVB7_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1913619.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHVB7_001603"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHVB7_001603"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR1913620.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHVB7_001604"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHVB7_001604"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR1913621.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHVB7_001605"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHVB7_001605"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR1913622.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHVB7_001606"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHVB7_001606"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR1913623.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHVB7_001607"
     CDS             complement(4084..5313)
                     /locus_tag="ACHVB7_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1913624.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHVB7_001608"
     CDS             complement(5377..5817)
                     /locus_tag="ACHVB7_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR1913625.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHVB7_001609"
     CDS             complement(5922..6692)
                     /locus_tag="ACHVB7_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1913626.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHVB7_001610"
     CDS             complement(6689..7615)
                     /locus_tag="ACHVB7_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1913627.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHVB7_001611"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHVB7_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR1913628.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHVB7_001612"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHVB7_001612"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR1913629.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHVB7_001613"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHVB7_001613"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR1913630.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHVB7_001614"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHVB7_001614"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR1913631.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHVB7_001615"
     CDS             13322..13669
                     /locus_tag="ACHVB7_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR1913632.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHVB7_001616"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHVB7_001616"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR1913633.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHVB7_001617"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHVB7_001617"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR1913634.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHVB7_001618"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHVB7_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR1913635.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHVB7_001619"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHVB7_001619"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR1913636.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKIEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="ACHVB7_001620"
     CDS             18978..20735
                     /locus_tag="ACHVB7_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR1913637.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHVB7_001621"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHVB7_001621"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR1913638.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHVB7_001622"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHVB7_001622"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR1913639.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHVB7_001623"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHVB7_001623"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR1913640.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHVB7_001624"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHVB7_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR1913641.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         6796 a         7676 c         6936 g         6796 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaatt
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgt
//
LOCUS       DAZPJR010000041        28041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPJR010000041 DAZPJR010000000
VERSION     DAZPJR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.042"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..133)
                     /locus_tag="ACHVB7_001625"
     CDS             complement(<1..133)
                     /locus_tag="ACHVB7_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1913642.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="ACHVB7_001626"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="ACHVB7_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            632..706
                     /locus_tag="ACHVB7_001627"
                     /pseudo
     CDS             632..706
                     /locus_tag="ACHVB7_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(703..2026)
                     /locus_tag="ACHVB7_001628"
                     /pseudo
     CDS             complement(703..2026)
                     /locus_tag="ACHVB7_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(2026..3642)
                     /locus_tag="ACHVB7_001629"
     CDS             complement(2026..3642)
                     /locus_tag="ACHVB7_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1913643.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3938..4654
                     /locus_tag="ACHVB7_001630"
     CDS             3938..4654
                     /locus_tag="ACHVB7_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1913644.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4651..6312)
                     /locus_tag="ACHVB7_001631"
     CDS             complement(4651..6312)
                     /locus_tag="ACHVB7_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR1913645.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="ACHVB7_001632"
     CDS             complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="ACHVB7_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR1913646.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="ACHVB7_001633"
     CDS             complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="ACHVB7_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR1913647.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7455..7527)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7767..8099
                     /locus_tag="ACHVB7_001634"
     CDS             7767..8099
                     /locus_tag="ACHVB7_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1913648.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8101..9315)
                     /locus_tag="ACHVB7_001635"
     CDS             complement(8101..9315)
                     /locus_tag="ACHVB7_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR1913649.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9416..10480
                     /gene="cbrA"
                     /locus_tag="ACHVB7_001636"
     CDS             9416..10480
                     /gene="cbrA"
                     /locus_tag="ACHVB7_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR1913650.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10477..11814)
                     /locus_tag="ACHVB7_001637"
     CDS             complement(10477..11814)
                     /locus_tag="ACHVB7_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1913651.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="ACHVB7_001638"
                     /pseudo
     CDS             complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="ACHVB7_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(13034..13651)
                     /gene="dgoA"
                     /locus_tag="ACHVB7_001639"
     CDS             complement(13034..13651)
                     /gene="dgoA"
                     /locus_tag="ACHVB7_001639"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR1913652.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13635..14513)
                     /gene="dgoK"
                     /locus_tag="ACHVB7_001640"
     CDS             complement(13635..14513)
                     /gene="dgoK"
                     /locus_tag="ACHVB7_001640"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR1913653.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="ACHVB7_001641"
     CDS             complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="ACHVB7_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR1913654.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15168..15800
                     /locus_tag="ACHVB7_001642"
                     /pseudo
     CDS             15168..15800
                     /locus_tag="ACHVB7_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341111.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15846..16658)
                     /gene="yidA"
                     /locus_tag="ACHVB7_001643"
     CDS             complement(15846..16658)
                     /gene="yidA"
                     /locus_tag="ACHVB7_001643"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR1913655.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16773..17171)
                     /locus_tag="ACHVB7_001644"
     CDS             complement(16773..17171)
                     /locus_tag="ACHVB7_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR1913656.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17411..19825)
                     /gene="gyrB"
                     /locus_tag="ACHVB7_001645"
     CDS             complement(17411..19825)
                     /gene="gyrB"
                     /locus_tag="ACHVB7_001645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR1913657.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19854..20927)
                     /gene="recF"
                     /locus_tag="ACHVB7_001646"
     CDS             complement(19854..20927)
                     /gene="recF"
                     /locus_tag="ACHVB7_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR1913658.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20927..22027)
                     /gene="dnaN"
                     /locus_tag="ACHVB7_001647"
     CDS             complement(20927..22027)
                     /gene="dnaN"
                     /locus_tag="ACHVB7_001647"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR1913659.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22032..23435)
                     /gene="dnaA"
                     /locus_tag="ACHVB7_001648"
     CDS             complement(22032..23435)
                     /gene="dnaA"
                     /locus_tag="ACHVB7_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR1913660.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23732..23812
                     /locus_tag="ACHVB7_001649"
     CDS             23732..23812
                     /locus_tag="ACHVB7_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913661.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24042..24182
                     /gene="rpmH"
                     /locus_tag="ACHVB7_001650"
     CDS             24042..24182
                     /gene="rpmH"
                     /locus_tag="ACHVB7_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR1913662.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24199..24558
                     /gene="rnpA"
                     /locus_tag="ACHVB7_001651"
     CDS             24199..24558
                     /gene="rnpA"
                     /locus_tag="ACHVB7_001651"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR1913663.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24522..24779
                     /gene="yidD"
                     /locus_tag="ACHVB7_001652"
     CDS             24522..24779
                     /gene="yidD"
                     /locus_tag="ACHVB7_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR1913664.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24782..26428
                     /gene="yidC"
                     /locus_tag="ACHVB7_001653"
     CDS             24782..26428
                     /gene="yidC"
                     /locus_tag="ACHVB7_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR1913665.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26534..27898
                     /gene="mnmE"
                     /locus_tag="ACHVB7_001654"
     CDS             26534..27898
                     /gene="mnmE"
                     /locus_tag="ACHVB7_001654"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR1913666.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6749 a         7582 c         7146 g         6564 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gtagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgactatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gtttaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggttact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtcc tggctatttt gccaatagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccgcgcctta tatccccgcc
      601 ctgaaggacg gggttttacg gcgcaccgga taaatttggt tatgcgccgt cagccgtgct
      661 aaagaatacg gaccagcacc caacggatgc cagtccacac aattaacgta actctggcca
      721 gtacgctttg ttggcctcca gcaactcatc cagaatagct ttggcgaccg aggcgctcgg
      781 taccgttttc gataacgtaa tcgcctgcca caggtgctga tatgaacgct gttcccaggc
      841 gtccacgacc agtttttcca ccgccacttg ctgactcatt aaccctttct gaaactgcgg
      901 gatatcgccg accaccagtg gttctggtcc gttgtgcccg accagacacg ggatctcgac
      961 catcgcttca tcatcaaagt tatggattgc cccgttgtta ggcacaatca gcaacatccg
     1021 ctcctgagtg ttgaaggcaa ttgccgccgc cagatcgacg atgtatgacg catgttcgtc
     1081 aatctccagc ttggccgccg ctgaatttcc tgccgctgta atggcgcggc aagcatcgaa
     1141 aacctgtttc tcgcgatgtt ccattcacct cattcgcgcg ggtatgttca gggttggaat
     1201 gctgaacaac ataatccggg aagagataat atttcagata ggtgttcggc aatgtatccg
     1261 ggtccagtgc ctggacatcc cgcgcttttg cataggtgtc gttccagctc gcttcagtat
     1321 gtgtatcttg ctgcggtgga acataaccgt gcttcgagac atattgccgc agctggggca
     1381 tcaggtcgtt gccctgcaaa tcttcaattg atgtccacca gccaaagtgg ttcaggccgt
     1441 agtagcgcac gcgcatctgt ttgcgatctt gcagcccaac aatttgcgcc atccggcttt
     1501 caataccgat tggcatgtca cagatgttga ggattttcgc attcgggcgc agacgacgcg
     1561 tggcttctgc gacaatggct gccgggttgg agtagttgag catccaggca tttggtgaat
     1621 atttttccat ataatccacc agttccagga cgccgccaat ggaacgcatg ccgtacgcta
     1681 ttccgccggg tccgcaagtt tcctgaccaa caacaccgtg gcgcagtggg attttttcat
     1741 ccagttcgcg catcgggtac ttgccgacgc ggatgtgcgc catgacaaaa tcaacgtcgc
     1801 tgaacgccac ttcaggatcg gtggtgtaac taaacgcaat gtccggcgct ttctctttaa
     1861 ggatgacttt acaggcttcg gcaatcactt cctgccgcgc gccatcgtta tcataaaatt
     1921 tcagtgcacg aagcgggaaa cgctcctgat tcgccaggag catcaacacg atgcctggcg
     1981 taaaggtgct tccaccgcct gcgacaacca ctgagaattt ggtcattata ctgcctccgt
     2041 aatggcaaca ttttctgctg attgatgaga attaattaag ctatcgagct gttcacgcag
     2101 ctgggataca tgcagaccaa tgatcacctg aatggcatcg ccactacgga agacgccgtg
     2161 cgctcccagc tttttaaaga cttcgtcatc cagcgtttgt gacatgtcat gcagtgcaat
     2221 gcgtagacgc gtcgcacaat tgttaatgct ggagatattg ccgaccccgc ccagggcttg
     2281 caggataccg gcagcctgat ccagctcttt ttttggctct gccgcggtgg tttggcctcg
     2341 cgaggctttg tattcggctt ttgagtagag tttcacttcc gcatcttcac gtcccggcgt
     2401 gcacatgttg aactgcagaa tcagggtgcg gaaaaccacg aagtacagca gggtaaagca
     2461 caacccaatg gcgatttggg tcagcatcat atccgcgtgg ttgctgaaca tcgggatcca
     2521 gttttgcggt aaaacctggt caatcagacc tccgcccatg ttgcccacca caccaaagag
     2581 atacatcacg gtcgacattg aggccgcaag cacagcgtgt accgcaaaca gcaacggtga
     2641 aatgaacagg aaggtaaatt ccagcggttc ggtaattccc accagcatgg cggttaaggt
     2701 tgcgggaatc agcaagcccg ccacttttac ccgattttcc ggtgcggcag tgaagtacat
     2761 cgctaaagaa atgcccacgg caccaaagat ttttgagtta ccgtgcaggg caaaaccgcc
     2821 ttccgggaac aacgatttca gcggctcggc gctcaaactg aactcttgca gatgctgcgc
     2881 ccagtacatc tgaatgccgc cttcaacagc tgccggaccc aagataaacg gtccgtagat
     2941 gaagtggtgt aaaccggttg ggatcagaat acgttcgagg aaggtgtaaa cccagacccc
     3001 aagcgcaccc gccgaacgca ggaacgcttg cagagattca atccccgttt gtacttttgg
     3061 ccagccgagc aatgtcagcc aggcacaggg gatcatcacc aggaaggcga taatcaccac
     3121 ataagacgtc ccctggaaaa tgccgagaaa aaccggcagt tttttatcga acagacggtt
     3181 atgcagcgcc gtcacaatgc cggaaatgat aattgcgccg ataatgctgg tatcgagggt
     3241 tttaatcccg gccatcattg tcagaccgct acctgccacc gcgtcctgag tgaaatcgac
     3301 gccgaagtag cttccccagg tcattcccat cgcgttgatg aaatagttcc aggtcaggaa
     3361 actcaccatc accgccagac aagcacgtcc ctgcgcttgc ttagcaaggc caatgggtaa
     3421 accgacagca aaaatcagcg gcatattacg gaataccgtc caaccgccct cttcaataat
     3481 gtgtacgatt tgcgcgaata aactgttcgg atcggtcagt gattccccga caaacatcgg
     3541 gttttgcagc aagatggcaa gacccaccac aatcccggcg aagggaaaca gcagcactgg
     3601 cgtaaacatc gcgccgccaa agcgttgaat ttgactgagc atttccacat cctcttttct
     3661 caattctgta agtaggaagc ctgattggtt atgcggcagg cctggagcaa ggataggaag
     3721 atgcgatcgg cagaacatga cgacccaatg ggattcgtga tcgctttcat gcttttcgtt
     3781 gggcggtaga gatctacgtt ttcgcgaaaa agtagacatc tgtgagcaca ggtacacagc
     3841 atcgaacaca tctttaaaaa aaagatgttt tttcaatcga ttaagcagaa cttgtgtgcg
     3901 cattacccgg gcttgcaggc aaaaaagaga tctagagatg atctacaaaa gcattgcgga
     3961 gcggttaaga attcgactta actccgcaga tttcacgcta aacagccttc ttccgggtga
     4021 aaaaaagctg gcggaagagt ttgcggtatc gcggatgacc atccgtaaag ccattgacct
     4081 gctggtagcg tgggggctgg tggtccgccg ccacggtagt ggcacttacc tggtgcgcaa
     4141 agatgtactg catcaaaccg ccagtctgac cgggctggtg gaggtgttaa aacggcaggg
     4201 aaaaactgtc accagccagg tgctgatttt tgaaatcatg cctgcgcctc cggccattgc
     4261 cagccagtta cggattcaaa tcaacgagca gatctacttc tcccgtcgcg ttcgttttgt
     4321 ggaagggaaa ccgctgatgc tggaagacag ctatatgccg gtaaaactgt tccgtaatct
     4381 ttcgctgcaa catctggaag ggtcgaagtt tgaatatatt gaacaagagt gcgggatttt
     4441 gattggcggt aattatgaaa gcctgacgcc ggtgctcgcc gatagactgc tggcgcggca
     4501 aatgaaggta gcggaacaca cgccactgct gcggatcacc tcgttgtcat atagcgagag
     4561 cggggagttt ttgaattatt cagtgatgtt cagaaatgcc agcgaatacc aggtggagta
     4621 ccatttacgg cgactccacc cggaaaagag ttaaccgata ctccagaaga gcaccgccag
     4681 taattggggg gtgataatgc gcaggaacat cactaacgga tagacagtgg cgtaagagag
     4741 cgccgccgca ccgctagttg gatgaagatt attagcaaac gccagcgccg gaggatcggt
     4801 catggaacct gccagcatcc cgcacatggt caggtaattc attttggcta acatccgcgc
     4861 cagaatgcca acagtaatca gcggaacggc ggtgatcagg gcaccataac caatccagct
     4921 tagcccttcg ccattgacca gggtattcac aaaatcccca ccagatttca gaccaacgac
     4981 cgagaggaac agcacgatcc ccagctcccg cagcgcgagg ttggcacttg gcggcataaa
     5041 ccagtacagc ttgccgatac tgccgatacg cccgaggatc aacgccataa tcagcagacc
     5101 gcccgccagc cccagtttca acgcggccgg gaatcccggc acaaagacgg gaatagaacc
     5161 taacaatacg ccaagcccga tgccaataaa cactggcagc atctgaacct gttgcagttt
     5221 ttgctgcgca ttccccagca cattggcaac ggcatcaatt gcggacggac gccccaccag
     5281 gttgaggata tcgccgaact gcaggctgat atcgccactg gcgaccagtt cgaccccggc
     5341 acggttcagg cgcgagataa caacgtcata gcgttcttta aagtgcaggt cgcgaatacg
     5401 ttttccgagc acgttttcat tggtgaccac cacacgctcg acgcgcaaat cagtgtcttt
     5461 cgtggacagc gaagtatcga cctcctgacc aatcaccagt tgcgcattat gtaaatccgc
     5521 tggctgaccc accagatgca gcaaatcgcc cagttggata atggtatctg gcgaaggaac
     5581 ttttagggtt tcttcgcgtt tcagacgcga gcagataatt ttgtcgccgt tgagaatcgg
     5641 tacatcttta atggctaaat catgcaggtt agggttctca acgcgaatat tgatagtctt
     5701 gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc tgagcttctg tctcgacatt
     5761 gacgcggaaa ataacccgca acatccacat ggtgaacaaa atcccgcaaa tgccgaatgg
     5821 atacgccatc gcgtaactca tccccatctg atcgaccatt tccattggtg tacccaggtc
     5881 gcgcaaaatc tgctgccctg cccccagcgc tggcgtattg gtaaccgcac cggagaaaat
     5941 ccccagcact accggcagtg gaatatcaaa cagtttatgc aggatggcgg taaccagacc
     6001 accgatgatg acgatcagaa cagcaaacag gttgaggcgt aatccggaga cgcgcaatga
     6061 ggcaaagaag cccggcccta cctgaatccc gatggtataa acgaacagga tcaggccaaa
     6121 ttcctgaata acatgcagca tatcgctact taatgtcatc cccgcctgag aaacaaaatg
     6181 gccgacgatg atcccaccaa acagcacgcc gccaatacct aatcctatgc cgcgaaattt
     6241 gacgttgccg ataaacaaac cgacgactgc caccaaagcc agaatactga ccgttaatgc
     6301 tatatcactc atcgtccatt tctcttgaga taacatttta attgctacgg attatggctg
     6361 aatcggtgaa gcagatttgc cttatagcgc acattattac gggaaagaac gtgccgaaat
     6421 atcttaaaca gtcgaaattt aacgcattgt tgaagaagaa agacaaaaaa agccccacca
     6481 gaatggcggg gcaaagagaa tagctagtta gctatttaac gcgggacgtt cgctgatagc
     6541 gatacgctgc gctgcgatgg gttcaggctc attacgaatt aaatcaatat gcagtaaacc
     6601 gtttacgaaa gttgcgccag agacttccat attttcagcc agcgtaaagc tcaggctaaa
     6661 tggctgattc ataagccctt gatgcagcca ttttttctct tcttttggct gctccggcgt
     6721 gccttttacg ctcaggcgcg taccttccag ttgaatctct aaatcttcct gacggaaacc
     6781 tgccagcgca agggtaatgc ggtagtggtt atcgtcgctt ttctcaatgt tgtacggcgg
     6841 gaagctctga ctttcaccgg cgttttgcag tgcgttggcc agtttgtcaa aaccgatcca
     6901 ttgacgcatc agtggggata aatcgaagtt acgcatagtc atttctcctt ctaagaagcg
     6961 agtaagtacc tgcaaatccg aagattcgca tatgctccct gacggcgagc atggagatgt
     7021 caggccgcgc caggcggcct taaggaatta gttgatttcg atacggcgcg gttttttcgc
     7081 ttccggaatc acgcgttcga gatcgatata cagcaaacca tttaccaggt tagcgccacg
     7141 aacatgaatg ttctcagcta actggaattt gcgttcaaag ttgcgttcag cgatgccctg
     7201 gtacagatag gtgcgctctt tttgttcgtc ggcgtgagca cctttcacca ccagcagatt
     7261 atcctgggcg gtaatttcca gttcgctctc agcaaaacca gccacagcga tagcaatgcg
     7321 gtaatggttt tcgtctacca gttcaacgtt atacggaggg tagccgccat tactctggct
     7381 ctggttgttt tctaagtggt taaacaaacg gtcaaatcca atagcagaac ggtaaagcgg
     7441 ggataaatca aagttacgca taatcaatag ctcctgaaat cagcgagaat gtaagacctt
     7501 ccacaatgga caggtcaggt agccagaaca cccatcaggc gcgtcctcat cgactacgat
     7561 gtaaaaatgg gtctggaaat gaacttttca agccttatca gcggactttt ttttgcagtt
     7621 tatggtctat tgcatagact gagggggcag cacgaaacgt taaagtgtga tagccgccac
     7681 agagcgacgt aatgatgatg ttattttcag caagggatcg ctataactca tcaggccaac
     7741 acccacgatc aacagatgaa tttattatga taagaaatgt gttgttagcg ttcatgatat
     7801 gcagcggaat ggcattactc ggaggatgct ccagcgtgat gtcccatacc ggagggaaag
     7861 aaggaacgta tcccggcacc cgcgccagcg ccaccatgat tggcgatgat gaaaccaact
     7921 ggggcactaa gtcgctggcg attctcgata tgccgtttac cgctgtgatg gacacgcttt
     7981 tattaccgtg ggatgtgttc cgcaaagaca gctcggtgcg ctcacgcgtt gaaaaaagcg
     8041 aagccaacgc gcaagccacc aatgcggtta ttccaccggc cagaatgcct gataactgat
     8101 ttagcgatca gtttcggtga tccacagctg gccaccttcc atccacgcca gccattgacc
     8161 atccggcgag aagaccacgg cgtccgcaga aggtggattg gcgtggtttt cggttaaata
     8221 ctcaacctcg ccgctttgcg catgggcaca agcaattcga ttatccagca caaagcccaa
     8281 ccattctcct gacggatgcc agttaaatgc agactgaata tccgttttgt tatgggttaa
     8341 ctggcgcggc tcgccgccct gtggcgagat aagccacagt tgcacaatgc cgttatcatc
     8401 acgcattaaa aacgcgattt gcgtaccctg cggattacag cgcacccagt ggcgcgggac
     8461 gttgactaac cccggataag cccgatggtg ggtaaaggtt aaacgtcgct gcacgacgcc
     8521 acgcggtggc gcgggcaggg tggtttccgt tccacttaac ggcgcatcac ctgccgcttt
     8581 ccagccagct tcatcttgcg gcaactcaac gataaacagc tccggcactt tctcaccctt
     8641 tggcgaaagt gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata
     8701 agcacgattg atttcatcac tgccaggctg cggcgtgggc gtggttttac tcaccagcac
     8761 acaccagtgg ctgccgctgt attcacgcgg atgctgcttt tgtacgttga ccggaccaaa
     8821 cggcgcagca acaccgacgt ttcgcaaatc cagcgccgga tcgagttcat gcattacatg
     8881 gtcgttatag gtaaagctca ccctttcacc gttcgggcta aagacatgca catggctgcc
     8941 gccgcgcagc gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac
     9001 cttgccgcct tcagcaatga ctccgcgccg atgatggaag tcgtaatgcc atgtttcatc
     9061 aggattttcc gggccgtgaa taaagacata tttctctgac ttcggatgaa cggtcaccac
     9121 gccgacgtgt gcgccctgtg acgcacagta gataacctcg acctcaccgg tatggatatt
     9181 cacacgctca atggtctcgc cggtaaacga cgccccagaa ggacgcacgt caaataccag
     9241 ccactggctg tcgggcgtcc aggtattggt attggtgagc aggtgattac ggggagcaaa
     9301 ggttatctgt ttcatggcgc ggtcctgata caaaacgggt ccagccatca tacttcacaa
     9361 ggacttcact ttcagacaat agtcttttgc catcgtgatg aatgtgagtg aaaagatgga
     9421 acatttcgac gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt
     9481 agccggcaaa atgcaggtga tcgcgctgga taaaaagcac cagcatggta ctgaaggttt
     9541 cagcaaacct tgtggcggtc tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg
     9601 gctgacgctt cctgtcgatg tgatcgctaa tccgcagatt ttcagcgtca aaaccgtcga
     9661 cgtcgccgca tcgctcacgc gtaactacca gcgaagctat atcaatatta atcgccatgc
     9721 tttcgacttg tggatgaaat cgctgatccc cgccagcgtt gaggtttatc acgacagcct
     9781 gtgtcgaaaa atctggcgtg aggatgataa atggcatgtc atttttcgtg cagacggctg
     9841 ggagcagcat attaccgccc gctatctggt cggtgccgat ggtgccaact cgatggtgcg
     9901 gcgacatctc tacccggatc atcaaatccg taaatatgtc gcaatccagc agtggttcgc
     9961 agagaaacat ccggtgccgt tctactcctg catctttgat aatgcgataa ctgactgtta
    10021 ttcatggagt atcagcaaag acggttattt tatctttggc ggtgcctatc caatgaaaga
    10081 cggtcagacg cgtttcacga ctctgaaaga gaaaatgagc gcctttcagt tccagtttgg
    10141 caaagcggtg aaaagcgaaa aatgcacggt gctgtttccc tcacgctggc aggattttgt
    10201 ctgcggtaag gacaacgcct ttctgattgg tgaagcggcg ggatttatca gcgccagctc
    10261 gctggagggg attagctatg cgctggatag cgcagacatt ctgcgttcgg tgttactgaa
    10321 gcagccagag aagctcaata ccgcttactg gcgcgccacc cgcaaactgc gtttaaaact
    10381 cttcggcaag atagtaaaaa gccgatgcct gaccgcaccg gctttaagaa agtggattat
    10441 gcgcagtggt gtggcacata ttccacagtt gaaggattag ccaacgcgct tcacatcgcc
    10501 caccagcagg atataagaga gcgcgccaat caacgcgacg gcggagatat aaaccagtgc
    10561 aggtgcgaaa ccgtaaccct gcgccaggta ccccaccacc agcggaacgg tgatgccgcc
    10621 cagaccaccg gcgaagttaa acacgccgcc ggttaaacca atcaggcgca tcggtgccag
    10681 agaagaaacc agcgaccagg taattgaagc aaaaccgtta ccgaagaatg ccagcgccat
    10741 caggcacata atcatcatcg gatcgttagt gtagttagcg cccataatgc aggtggagat
    10801 cagcaagccg cagataatcg gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac
    10861 cagcagatcc gctacccagc cagagagcag gacgccgaca aacgccgcga ggaatggcac
    10921 cgtggtcata aagcctgctt tcagcgccgt gattcctttt tcctgggtta aatagttcgg
    10981 gaaccaggtt aagaaaaacc acagtgtaga agccaccgca aattgcccaa gatagacgcc
    11041 gatcagttta cgatggaaca ccagtttcca gtctttggct gttaacggct gacgtgcctc
    11101 tttcttcact ggcgcatcac catccaccag accgccgcca tcacgaatgt aatccagttc
    11161 agctttgctg atgcctttgg tcaggcgcgg cggctgataa accttaaacc aaatcagcga
    11221 ccagataatg ccgataccac cagtcacaat gaacacccag tgccagctca acatctcctg
    11281 aatccagatc agcagcggag tcaaaaacgc cagaccgaca aactgaccag acgtataaaa
    11341 accaacggcg gaggcgcgtt catgttccgg gaaccagctg gtcaccatcc ggttattggt
    11401 cgggaaagcg ggcgcttcga aaataccggt tatcgcacgc agaccaatta atgacattaa
    11461 tcccgtggca aagccctgga ataaagtcgc cactgaccag ccaaatatcg caataaaata
    11521 agtcacgcga gaacctacgc gatctaaaaa ccaaccgccg gggatctggc atagcgtata
    11581 aagccaggcg aaggccgaaa atacatagcc catttccgct ttcgtaatgc cgaactcttc
    11641 ctgaatatgg gcggaagcca cggccaggtt agcgcggtca acataacaaa tgaccaccgt
    11701 aataaagatc atcaccagcg tcagataacg ccgacgcccc ggctttgctg cattaacggg
    11761 aatatccata gcgagctttc tccagatttt gggcatagcg aagccgctca ccatgccctg
    11821 taatttacag agggttatat ttttgtattg ctgttttagt gcccgatgag gggcttacgt
    11881 ggcaggaatt accactctgc tacgctgtta tcttcatgac gccagagtgg attacgccag
    11941 tccggggcat ttttactgaa ctcaataact ttagcttcgt cgatttccac gcctaagccc
    12001 ggtttcgtta gcggtttaaa gaagccgcca accatgctga agtcttcttt gtttttcaca
    12061 aagtcgagta actccgcgcc tttgttgtaa tgaattccca tactttgttc ctgaagtacc
    12121 gcgttatagg aaacaaagtc gatatgcaga caagccgcca gtgcaatcgg tccgagcgga
    12181 cagtgcggcg caagggtcac gtcataggct tctgccattc cggcaatttt gtagcattcg
    12241 gtaataccgc ctgcatggga gagatccggt tgtagaatcg aaataccgcc tgcctccagc
    12301 acgcgtttaa aatcgaaacg tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt
    12361 tgtgccgcca atttcgggta gtattcggct tgttccgcca gcaccggctc ttcaataaac
    12421 agcgggcgat acggctccag ttctttaatc agcactttcg ccattggcgc gctgacgcga
    12481 ccgtggaaat caagaccaaa ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg
    12541 gtgttgactg ccgcatctac cgcgcgggag ttatcaatta gccccagttc ttcacaaccg
    12601 ttcagtttga aggtatcgaa gccgatttcg cgcagggttt taataccgtc gatcacatcc
    12661 gccggacgat cgccgccgac ccaactgtag gctttaattt tgtcgcgaac caggccgccc
    12721 atcagttgcc agaccgacgc attcagcact ttacctttga tatcccataa cgcctggtcg
    12781 atcccggcaa tggcgctcat caggattgga ccgccacgat aaaatccggc gcgatacatc
    12841 acttgccata agtcattgat gcgctatgga tcctgaccaa tcaaatagtc acccagctcg
    12901 tgaactgccg cttccaccgt gcgggcgcgg ccttcaatca ctggctcgcc ccagccgacg
    12961 acgccttcat cggtttcaat tttcaggaac atccagcggg gaggtaaacg atacgtggta
    13021 attttggtga ttttcattgc actgcctctc gatacgcctt aacaaatgct gctgcctgct
    13081 gcgcggtgcg ttctacggac tgcccggcgc gatagagatc gctgcctaag cccgccccag
    13141 cacaacctgc gtctatccac tgcgccaggt tttctggcgt cacgccgcca acggcaaaga
    13201 ctgcgatgtc cgatggcaat accgctttta acgctttgat gtattgcgga ccaaaagccg
    13261 atgacggaaa tattttcagc gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg
    13321 ccgtcgcgca gccggggcag acggtcatgc cgtagcccac cgcacggcgg atcacttcac
    13381 tatggatatt gggcgtaacg atgagctgac agcccatcct ggcgagcgca tcgacctgtt
    13441 caggtttcag taccgtacct gcgccaatca acgccttgtc gccatacgca tcaacgatgg
    13501 cgggaatgct ttgctcccat tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg
    13561 cgtcaatcac cgcgccaaca tgcgccagcg cctcgtcggg cttaatgccg cgcaaaatgg
    13621 cgatcagcgg aagtttagtt tgccactgca tgagcgatgc tccttatacc agcctgaaat
    13681 gccgtgtcgc ccgccaccgc cgtcacgtcg caacccatcg cctgaaaggc ttgctggtag
    13741 cgcgcggtca acgatgttcc ggcgacaagg gtgatggcgt gttgatgggc cacatagtcg
    13801 cgcatactgg cgacctctgc gccaatcaac aaaccagaga gaaattcact gacctgttca
    13861 cggggaagtg ttcccagcac atgcgaggca cgaacttcaa aaagctgcgg caatatggcg
    13921 ggcgtattaa gaccacgctc aaggccagcc gtgaaggcat cggcagagtt ttcctgcggc
    13981 ggcaaacctg caccaatcaa tgagtgattt aacagtaaat gatgtaattc accggtcatc
    14041 acggtgcgaa aatcgttgat ttgctggcta tcggcctgca cccatttgca atgcgtaccg
    14101 ggcatgacat aaagagagga aggagccaga gctcgcgcgc cgatcaattg agtttcttcg
    14161 ccgcgcatca cattgtggtt atcatcatga gagacgcata atccgggaat aatccagata
    14221 ttgtcgccaa cagacgttaa ttgttcgcca atagacgaaa aatgggcagg aacagataaa
    14281 tacggagcaa ctttccagcc gacattgctg ccgaccattc ctgccattac cactggcgta
    14341 ttctcttcac gccagtcggt cgtgacttct gctaacaccg cagccggaga ttttccgttc
    14401 aggcgcgtga cgcctgcttc tgattgcctg ctctccaggc agtggtcgcc ctgataaagc
    14461 caggcgcgca gattggtcga tccccagtca attgcgatgt agcgagctgt catgtgattt
    14521 cctttaacct tcgtgtcgag ctggcgatca tggtaagcgc cgcctgctct gccgcatcgc
    14581 cgtcctgatg ccgtatcgca tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt
    14641 tggcctcatc gcccatccag gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa
    14701 tgctaagttg ctgtaacacc gggttatgca ccgactgcag caccgcctcg tggtagcgaa
    14761 tatccgcttc gttaaacgct tcgcggtcct gattgttggc aatcatctcg ttcagcgccg
    14821 attcaatctg cgccagatcg ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg
    14881 gttccaccag atttcgcact tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt
    14941 ccagcaccca ttgcagtacg tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa
    15001 acgcgccgcg ataacgtttc atttcaatca gccgtttcgc catcaacgaa cggaacacct
    15061 cacggatgat gttgcgcgag gttgcaaact cctcacagag ttccgcctca gccggaagcg
    15121 gcgagcctgg tacgtatttg ccgtgaacga tctgtttacc cagcgtaatg acgatgcgat
    15181 cggttttatt gagagtcatg gagagtcctt gtgctctgta tgttcttctc tactttaccc
    15241 cgatcgatgc ataatcagga aagtatcaat cgcagtcaga caccatcacg gggctatcgc
    15301 aggcaaagga tagtaatgga acaaaaggct atgtttttgt aggggaatcg ctggattacc
    15361 ttatcacaga tggtgccgat gacatcgtta agatgctcaa ggatccagct cttaaccggc
    15421 acaatattca ggttgctgat gacgcaagat ttgttttaaa tgcggggaaa aagaaattta
    15481 ccggcacaat atcactctat tacaactgga gcacagaaga agaaaaagca cgagtgacac
    15541 aatatcgttt tgcttgtggt gttcagcact gtacccggac agtagaaaac ctggaaggta
    15601 ctatccatga gaaaaataaa aacatggatt actcaaacgt aatggctttc taccatccat
    15661 ttaaagtgcg attttatgaa taccattcac ccagaggcat tccggatggt gtttccgcag
    15721 cattactgcc agtgactgtt acgctggaca tcattactgc accgctgcaa tttctggttg
    15781 tatatgcagt aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc
    15841 acagattaat tcagcacata cttctcaata gcaaacgcca cgccatcttc aaggttagat
    15901 ttggtgacaa agttcgccac ttctttcacc gaaggaatag cgttatccat cgccacaccg
    15961 acgcctgcat attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc catgatttct
    16021 tccggtttaa tacctaacac gtcggccagt gatttcacac ccgtaccttt gttaacgcgt
    16081 ttatcgagga tttcgaggaa gtacggcgca cttttcagca cggtatattt ctctttcact
    16141 tcctgcggaa tacgcgcgat agcctggtcg aggatggcgg gttcatcaat catcatcact
    16201 ttcaggaact gggtattggg gtccattttc tccgcttcgc agaacaccag cggaatagtg
    16261 gcaacgaaag attcatgcac tgtgtagtag ctgatatcgc ggttggcggt gtacagcgtg
    16321 gtgcggtcca gggcgtggaa atgagaaccg acttcgcgag agagtttttc caggaaacga
    16381 tagtcgtcat agctgagagc agtttgcgcc acggtgctac catcagctgc cttctgtact
    16441 agcgcgccgt tataagtaat gcagtagtcg cccggctgtt ccatatgcag ctctttcagg
    16501 tagttgtgca cacctgcata cgggcgaccc gtcgttagca cgacattcac gccacgggcg
    16561 cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg
    16621 gtgccatcca tatcgatagc aatgagtttg atagccatga gttccccagg tagattggtt
    16681 cctgacccat gctaacgcga ttccgctcaa aaatcagtac aacacccgag ggaaaagggg
    16741 gatgcaacgc gggttgcatg ctccccattt gcttagcgga agagtttccc tttcagcagt
    16801 tccatacctg cggaaagcag atcgttattg gcttgtggtg acacttcacc ttgcggtgag
    16861 agcgcatcaa taatcttcgg caattgctct gccagtaaac tggaagctgt actggtatcc
    16921 acgccaagtt tttgcccgag atcggacacc gcatttgtgc cgagcgccga ttccagttgc
    16981 tcgccactaa ccgattgatt gccctgttga ttactcagcc aggttgagag aatggcccct
    17041 aagccgccac tttgcagttt ttccagcagc acctgaatgc cgccctgctc ctcaacccaa
    17101 cttaaaatag cctgatattt ccccgcatcg cctttcagaa aggcaccgac aacttcatca
    17161 aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg acccaaaaag tatagatttg
    17221 cgggatgata attgcggatt gcagaaataa aaagggcgga gatgatctcc gcccttttct
    17281 tatagcttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat catgaaaatt
    17341 caatacattg caagattttc gtaggcctga taagcgtagc gcatcaggca cgctcgcatg
    17401 gttagcgcca ttaaatatcg atattcgccg ctttcagggc gttctcttca ataaacgcac
    17461 ggcgcggttc aacggcgtcg cccatcagcg tggtgaacaa ctggtcggca gcaatcgcat
    17521 ctttaacggt aacgcgcagc atacgacggc tttccgggtc catggtggtt tcccacagct
    17581 gttccgggtt catctcgccc agacctttat aacgctggat ggagaggccg cgacgggact
    17641 ctttcaccag ccagtccagc gcctgctcga agctggctac cggctgacga cgctcgccac
    17701 gttcgataaa cgcatcttct tccagcaagc cacgcagttt ctcacccagc gtgcagatac
    17761 gacgatattc gccaccggtg ataaactcgt gatccagcgg atagtcagta tccacaccgt
    17821 gggtacgcac gcgaacaatc ggttcaaaca ggttttgttc ggcattggtg tggacatcaa
    17881 acttccactg gctgccgtgc tgttctttgt cgttcagttc gctgaccagc gcgttcaccc
    17941 agcgggtaac ggtctgctca tcagagaggt cggcttccgt cagcgtcggc tgatagataa
    18001 gctctttcag cattgctttc ggataacgac gctccatgcg gttgatcatt ttctgcgtcg
    18061 cgttgtactc agacaccaat ttctctaacg cttcgccagc caatgccggt gcgctggcgt
    18121 tggtgtgcag cgttgcgcca tccagcgcga tagagatctg gtactgatcc atcgcttcgt
    18181 cgtctttaat gtactgttct tgcttgcctt tcttcacttt gtacagcggc ggctgagcga
    18241 tgtagacgtg accgcgttca acgatttccg gcatctgacg atagaaaaag gtcaacagca
    18301 gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat gatgatgatg ctgtgataac
    18361 gcagtttgtc cgggttgtac tcgtcacgac cgataccaca gccaagcgcg gtgataagcg
    18421 tcgccacttc ctgagaagag agcatcttat cgaagcgcgc tttctcaacg ttgaggattt
    18481 tacccttcag cggcagaatc gcctggttct tgcggttacg cccctgcttc gcagagccgc
    18541 ccgcggagtc cccttccacc aggtacagtt cggaaagcgc cggatcgcgt tcctggcagt
    18601 ctgccagttt gcccggcagg cccgctaagt cgagcgcacc tttacggcgg gtcatttcac
    18661 gcgcgcgacg cgccgcttca cgggcacggg cagcatcgat aattttgcca accacgattt
    18721 tcgcgtcggt tgggttttcc agcaggtatt ctgccagcag ttcgttcatc tgctgttcaa
    18781 ccgctgattt cacctcagaa gaaaccagtt tgtctttggt ctgggaggag aatttcgggt
    18841 ccggcacttt cacggaaacg accgcaatca ggccttcacg cgcatcgtca ccggtggcgc
    18901 taactttggc ttttttgctg tagccttctt tgtccatgta ggcgttcagg gtacgggtca
    18961 tcgccgcacg gaagcctgcc aggtgagtac cgccgtcacg ctgcggaatg ttgttggtaa
    19021 agcagtagat gttttcctgg aagccatcgt tccactgcaa cgccacttcg acgccaatac
    19081 cgtctttttc agtggagaag tagaagatat tcgggtggat cggcgttttg ttcttgttca
    19141 gatattcaac gaacgccttg atgccgcctt catagtggaa gtggtcttct ttgccgtcgc
    19201 gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa cgacaactca cgcagacgtt
    19261 tcgccagaat ttcatattcg aactcggtca cattggtgaa ggtttcgagg ctgggccaga
    19321 aacgcaccat ggtgccggtt ttttcagtct cgccggtaac cgccaacggt gcctgcggta
    19381 caccgtgttc gtagatctga cggtgaattt taccctcgcg ctggataacc agctccagtt
    19441 tttgcgacag ggcgtttact accgaaacac caacgccgtg cagaccgccg gacactttat
    19501 aggagttatc gtcaaattta ccgcctgcgt gcagaacggt catgatcact tccgccgccg
    19561 atacgccctc ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc tgtacagaga
    19621 cagagttatc ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg agcgcttcgt
    19681 cgatagcgtt atctaccacc tcgaatacca tgtggtgcag accggtgccg tcatccgtgt
    19741 cgccgatata catacccggg cgcttacgca ccgcatccag ccctttcagg actttgatac
    19801 tggaggagtc ataagaattc gacatcaacg tttctcgctc atttatactt gggttaatcc
    19861 gttattttac ccttttccac ggtaaacatc ttcgaatttt cgtccgacat gtctataacg
    19921 tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa gcggctggca
    19981 agcagcccgc gacgctcatc atcaagctca gaggcaaaat catctatcag gtagagacac
    20041 cgccgcccgc tttcacgggt gaggaactct ccttgcgcca gacgtaaggc gcacatcaac
    20101 agcttaagct gcccacgcga taaggtatct tccaccggcg caccgtcggc gcgaatgcgt
    20161 aagtccgctt tatgcgggcc atgcgcggtg taggttagct ggcgatcgcg ttcaaaatta
    20221 cgttccagca cctcagcata ttctgtctct ttctcccagc cgcgctggaa agagaaagtc
    20281 agagaaaact cagggagaaa ttgcttacaa gtatcggcca tatcagccgc gataccggcg
    20341 ctatactccg cgcgccaggt gctgatttgc tccgccagcg ggatcagctc tttatcccac
    20401 gggcgtagct gttcgtaacg tgtcacctgg cgcagcgccg cattgcgctg cttgagcaat
    20461 cgcttgagat tgctccaggc ggtgaaaaat ccgggttcgt tgtgaaagca tccccagtcg
    20521 aggaatgctc ttctgtattt ggggccgccg ttgagtaaag taaacccttc tggcgttatc
    20581 aactgcattg gcatcaggtg cgccagttcc gcgaccttat gcccgtctgt accgtcgatg
    20641 cggactttgc tgtcgccctg tttgtctttg gttaagccaa tcgctgtctc gcgctcttcg
    20701 ccctgtaatc gcccgtggag aacaaacgcc tcctgctcat ggcgaatgac gcgaccaatc
    20761 tgcaaactgc gaaacgcccg accatggccg agcgtataga tggcttccag cacgctggtt
    20821 ttgccactgc cgttggcacc taccagaaag ttaaagccgg gagataaagc gagatccgcg
    20881 gtttcaatgt tgcggaaatc gcggatcaac aagcgggtga gggacattac agtctcattg
    20941 gcatgacaac ataggcagcc gactgtgatg cggcatcttc aatctgcacg ctggaaaccg
    21001 aatcggtcag catcatgcgg acgttttcac atttcagcgc gttcagaaca tccagcacgt
    21061 agctgacgtt gaagccgatt tccatctccg caccgctata ggtaacgtcg aggatctctt
    21121 ccgcttcttc ctgttccggg ttgttggcgg tgattttcag ctggttttcg ctgacataaa
    21181 gacgtacgcc gcggaatttc tcgttagaga gaatcgccgc gcgagcaaac gcctgcttga
    21241 gcagatcgca gccagcttcc agatgtttgt ccgggttctt cggcagaacg cggcgataat
    21301 ccgggaagcg accatccacc agtttggagg tgaagataaa gtcgccaacg tgggcgcgaa
    21361 tgttgttgct gccaatctgt acgcgcagcg gattgtcgcc gccgtcgagc atacgcatca
    21421 gttcaatcac gcctttacgc ggcacgatca ccgaatggct tggcaaagat tgaccaattg
    21481 gcattgaaca gactgccaga cggtggccgt cggtcgccac ggtgcgcagt tcttcgcctt
    21541 cggtttcaaa cagcataccg tttaagtaat agcgaacgtc ctgatgcgcc atagaaaact
    21601 gggtcgcttc aatcagacgc ttcatcgtcg cctgcggcag ggtaaattcg acttcgctct
    21661 gccagtcatc gaggttcggg aaatccgccg ctggcagggt agacagcgaa aaacggctac
    21721 gcccggagcg caccagcatc cgttcacctt ccagctgtac ggcaatttcc gcgccttcag
    21781 gcagaccacg gcagatatca aagaatttgc gcgccggaac ggtcgtcgct cctggctcgt
    21841 gtggctgaac cagcgcaacg cgtgccacca tttccatctc gagatcagta ccggtcagcg
    21901 acaacgtacc gtcagcaacc tgtaacagca gattaccgag aatcggtagc gtaggacgac
    21961 cacctaacgg cccgctcacc tgttgtagcg gttttaataa atgctcacgt tctacggtaa
    22021 atttcatagg tttacgatga caatgttctg attaaatttg aaaaatcttc tttgatatcg
    22081 tggctctctt cacgcaactg ctcgatctta cggcaggcat gaagcaccgt cgtgtggtca
    22141 cgaccaccaa acgcatcgcc aatctccggc agactgtggt tagtcagctc tttcgccagc
    22201 gccatcgcca tctggcgcgg acgcgccacc gagcgggatc gacgcttgga aaggagatcc
    22261 gcgactttga tcttgtagta ctccgccacc gtcttctgaa tattgtcgat ggtgaccagt
    22321 ttttcctgca atgccagcaa gtcgcgcagt gcctcacgca cgaagtcgat ggtgatcgcc
    22381 cgtccggtaa agttggcgtt agcaataacg cggttcagcg ccccttccag ctcacgtacg
    22441 ttagatcgta gacgcttggc gataaagaac gccacttcgc ctggcaaacg aatgtcattt
    22501 tcgtcggcct ttttcatcag gatcgccacg cgggtttcca gctctggcgg ttcgatcgcc
    22561 acagtcagtc cccaaccgaa gcgggatttc aaacgatcct caacgccgtt gatctctttc
    22621 ggatagcgat ccgaggtgag aatgatctgt tgattacctt ccagcagggc gttgaaggtg
    22681 tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa actgaatatc gtcgatcagc
    22741 agtgcatcta cggaacggta gtagcgttta aactcttcga tcgcgttgtt ttgcagggct
    22801 ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa ccactttggc attcggcttg
    22861 cgcgccataa tgccgttacc caccgcatgc agcaggtgag ttttacccag acccgtgccg
    22921 ccataaagga acaatgggtt ataggcaccg ccagggttat ccgccacctg gcgagccgcc
    22981 gcgcgcgcca gttggttaga tttaccttca acgaagttat caaacgtgtg tttgacgttt
    23041 acgttagaac gataggtcgg ttctgccggg gccgggacgt tatcccaacc tgagcgcgta
    23101 gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc gacgttgctc
    23161 gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa acgcagctgt
    23221 ggggcatccg ctccgcagaa actggttagc agtccattga tattattaag gtacttgtcc
    23281 cgtacccaat cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt atcgctcagt
    23341 tccgcctgca atgggcgtat ccacatactg aattctgtgg ctggtaactc atcctgcaat
    23401 cgggcaagac actgctgcca aagcgaaagt gacacggcgg actccactcg aacaaaagtc
    23461 gataatgact aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag accctgcttg
    23521 agggtgacgc acgaaccgct gtctgcggtt atatgcccga tcaagatcct gcaaaacgat
    23581 cgggaccgcg gatcatagcc taaactgcgc aagagatctt ctgtttctca cagattttcc
    23641 cgatttatcc acaggacttt ccagacctcg ctaagtgtaa acgatcctgc cgcgaggcgg
    23701 gcacgattta cgccgcatat tggaaaattt aatgaccata gacaaaaatt ggcttaatcg
    23761 atctaataaa gatctaggac gatccttgcg ctttacccat cagcccgtat aatcctccac
    23821 ccggcgcgcc atgctggttt tcactggtgt gaggtcgtac attttccctg cgaaaaggtg
    23881 cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg agtgtacaat tattacaatc
    23941 cggcctcttt aatcacccat ggcttcggtg tccatcgttt catttttcgg cggatatcca
    24001 ataaagccat tgaatttatt caagtttagg tagaaatcgc catgaaacgc acttttcaac
    24061 cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct actaaaaatg
    24121 gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc gtttctaagt
    24181 aataaagcta acccctgagt ggttaagctc gcatttccca gggagttacg cttgttaact
    24241 cccagtcaat tcacattcgt cttccagcag ccacaacggg ctggcacgcc gcaaattacc
    24301 attctcggcc gcctgaattc gctggggcat ccccgtatcg gtcttacagt cgccaagaaa
    24361 aacgttcgac gcgcccatga acgcaatcgg attaaacgtc tgacgcgtga aagcttccgt
    24421 ctgcgccaac atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa aggggttgcc
    24481 gacctcgata accgtgctct ctcggaagcg ttggaaaaat tatggcgccg ccactgtcgc
    24541 ctggctcgcg ggtcctgata gccctcattc gggtctatca acgcctgatt agtccgctac
    24601 tcgggccgca ttgtcgtttc actccaacct gttcaagcta cggaattgag gcattgcgca
    24661 ggtttggagt gataaaaggc agttggttga cggtgaaacg cgtattaaaa tgccaccctt
    24721 tacaccctgg tggtgacgat cccgtcccgc ccggaccatt tgataccaga gaacactaac
    24781 gatggattcg caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt tcatgatctg
    24841 gcaagcctgg gagcaggata aaaacccgca acctcaggcc caacagacca cgcagacaac
    24901 gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc aggggaaact
    24961 gatctcggtt aagaccgacg tgcttgatct gaccatcaac acccgtggtg gtgatgttga
    25021 gcaagctctg ctgcctgctt acccgaaaga gctgaactct acccagccgt tccagctgct
    25081 ggaaacttca ccgcagttta tttatcaggc acagagcggt ctgaccggtc gtgatggccc
    25141 ggataacccg gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg cttatgtgct
    25201 ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg caggcaacac
    25261 gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca actacaacgt
    25321 gcagaacgct ggcgagaaac cgctggaaat ctccaccttt ggtcagttga agcaatccat
    25381 cactctgcca ccgcatctcg ataccggaag cagcaacttc gcactgcaca ccttccgcgg
    25441 cgcggcgtac tccacgcctg acgagaagta cgagaaatac aagttcgata ccattgccga
    25501 taacgaaaac ctgaacatct cttcgaaagg tggttgggtg gcaatgctgc aacagtattt
    25561 cgcgacggcg tggatcccgc ataacgacgg taccaacaac ttctataccg ctaatctggg
    25621 taacggcatc gccgctatcg gctataaatc tcagccggta ctggttcagc ctggtcagac
    25681 tggcgcgatg aacagcaccc tgtgggttgg cccggaaatc caggacaaaa tggcagctgt
    25741 tgctccgcac ctggatctga ccgttgatta cggttggttg tggttcatct ctcagccgct
    25801 gttcaaactg ctgaaatgga tccatagctt tgtgggtaac tggggcttct ccattatcat
    25861 catcaccttt atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt acacctccat
    25921 ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc tgggcgatga
    25981 caaacagcgt atcagccagg aaatgatggc gctgtacaaa gctgagaagg ttaacccgct
    26041 gggcggctgc ttcccgctgc tgatccagat gccaatcttc ctggcgttgt actacatgct
    26101 gatgggttcc gttgaactgc gtcaggcacc gtttgcactg tggatccacg acctgtcggc
    26161 acaggacccg tactacatcc tgccgatcct gatgggcgta acgatgttct tcattcagaa
    26221 gatgtcgccg accacagtga ccgacccgat gcagcagaag atcatgacct ttatgccggt
    26281 catcttcacc gtgttcttcc tgtggttccc gtcaggtctg gtgctgtact atatcgtcag
    26341 caacctggta accattattc agcagcagct gatttaccgt ggtctggaaa aacgtggcct
    26401 gcatagccgc gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat aagggcggtc
    26461 agttgaccgc cttttttctt ttcgtagggt ggataagcac cgcgcatccg ccacacaaag
    26521 caacaggaac atcatgagcg ataatgacac tatcgtagcc caggccacgc ctccgggacg
    26581 tggcggcgtt ggcatcctgc gcatctccgg cctaaaagcc cgtgaagttg ccgaaaccgt
    26641 gctgggtaaa ctgcctaagc cgcgctacgc cgattatctt ccgtttaaag acgccgacgg
    26701 cagcgtgctc gatcagggga ttgcgctatg gttccctggc ccgaactcgt tcaccggcga
    26761 agatgtgctg gaactgcagg gtcatggcgg tccggtgatc ctcgacctgc tgttaaaacg
    26821 cattctgacc attcccggcc tgcggattgc tcgccctggt gagttttccg aacgcgcgtt
    26881 tcttaacgat aaactcgact tagcccaggc cgaggcgatt gccgatctta tcgacgccag
    26941 ttcggaacag gcggcccgtt cggcacttaa ctcgctgcaa ggcgcattct ccgcacgggt
    27001 caaccatctg gtggaagccc tcacccactt gcgcatttac gtcgaagcgg caattgattt
    27061 ccctgatgaa gagatcgatt tcctctccga cggcaaaatt gaagcccagc ttaatgacgt
    27121 gattgccgat ctcgatgcag tgcgtgctga agcacgtcag ggtagtttgt tgcgcgaagg
    27181 gatgaaggtg gtgattgccg gacgtcctaa cgccggtaaa tcgagcctgt taaacgcgct
    27241 ggcgggccgt gaagcagcaa tcgtaactga tatcgccgga accacacgtg acgtgctgcg
    27301 tgagcatatc cacattgacg gaatgccgct gcatatcatc gataccgctg ggctacgtga
    27361 agccagtgac gaagtggaac gtattggtat cgagcgcgcg tggcaggaaa ttgaacaggc
    27421 cgaccgcgtg ctgtttatgg tcgatggcac cacaacagac gccgtggatc cggcagagat
    27481 ctggccggaa tttatcgccc gtctgccagc gaaactaccg atcaccgtgg tgcgcaataa
    27541 agccgatatc accggcgaaa cgctgggaat gagtgaagtg aacggtcacg cgttaattcg
    27601 tctctcggca aggacaggtg aaggcgtgga ggtgctgcgt aaccatctca aacagagcat
    27661 gggctttgac accaacatgg aaggcggctt cctggcgcgt cgtcgccacc tacaggcgct
    27721 ggaacaggca gcggagcatt tgcagcaagg taaagcgcaa ctgttaggtg cctgggcggg
    27781 tgaactgctg gcggaagagt tgcgcctggc acagcagaac ttaagcgaaa tcaccgggga
    27841 atttacctct gatgatttgc tggggcggat tttctccagc ttctgtattg gtaagtaacc
    27901 gcgcttacga agccgtattc tgactgtcag atgcggcttc gcttcattgt tagcactcct
    27961 gttattcctc aacccttttt ttaaacatta aaattcttac gtaatttata atctttaaaa
    28021 aaagcattta atattgctcc c
//
LOCUS       DAZPJR010000042        27867 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPJR010000042 DAZPJR010000000
VERSION     DAZPJR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27867
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.043"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            82..744
                     /gene="acrS"
                     /locus_tag="ACHVB7_001655"
     CDS             82..744
                     /gene="acrS"
                     /locus_tag="ACHVB7_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR1913667.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(747..926)
                     /locus_tag="ACHVB7_001656"
     CDS             complement(747..926)
                     /locus_tag="ACHVB7_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR1913668.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(1010..1894)
                     /gene="yhdJ"
                     /locus_tag="ACHVB7_001657"
     CDS             complement(1010..1894)
                     /gene="yhdJ"
                     /locus_tag="ACHVB7_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1913669.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1980..2276)
                     /gene="fis"
                     /locus_tag="ACHVB7_001658"
     CDS             complement(1980..2276)
                     /gene="fis"
                     /locus_tag="ACHVB7_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR1913670.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2302..3267)
                     /gene="dusB"
                     /locus_tag="ACHVB7_001659"
     CDS             complement(2302..3267)
                     /gene="dusB"
                     /locus_tag="ACHVB7_001659"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR1913671.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3596..4477)
                     /gene="prmA"
                     /locus_tag="ACHVB7_001660"
     CDS             complement(3596..4477)
                     /gene="prmA"
                     /locus_tag="ACHVB7_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR1913672.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4489..5940)
                     /gene="panF"
                     /locus_tag="ACHVB7_001661"
     CDS             complement(4489..5940)
                     /gene="panF"
                     /locus_tag="ACHVB7_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR1913673.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5930..6172)
                     /locus_tag="ACHVB7_001662"
     CDS             complement(5930..6172)
                     /locus_tag="ACHVB7_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR1913674.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6281..7630)
                     /gene="accC"
                     /locus_tag="ACHVB7_001663"
     CDS             complement(6281..7630)
                     /gene="accC"
                     /locus_tag="ACHVB7_001663"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR1913675.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7641..8111)
                     /gene="accB"
                     /locus_tag="ACHVB7_001664"
     CDS             complement(7641..8111)
                     /gene="accB"
                     /locus_tag="ACHVB7_001664"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR1913676.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9089..10063)
                     /locus_tag="ACHVB7_001665"
     CDS             complement(9089..10063)
                     /locus_tag="ACHVB7_001665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR1913677.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10215..12155
                     /gene="csrD"
                     /locus_tag="ACHVB7_001666"
     CDS             10215..12155
                     /gene="csrD"
                     /locus_tag="ACHVB7_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR1913678.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAAQLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12460..13503
                     /gene="mreB"
                     /locus_tag="ACHVB7_001667"
     CDS             12460..13503
                     /gene="mreB"
                     /locus_tag="ACHVB7_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR1913679.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13569..14672
                     /gene="mreC"
                     /locus_tag="ACHVB7_001668"
     CDS             13569..14672
                     /gene="mreC"
                     /locus_tag="ACHVB7_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR1913680.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14672..15160
                     /gene="mreD"
                     /locus_tag="ACHVB7_001669"
     CDS             14672..15160
                     /gene="mreD"
                     /locus_tag="ACHVB7_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR1913681.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15169..15762
                     /gene="yhdE"
                     /locus_tag="ACHVB7_001670"
     CDS             15169..15762
                     /gene="yhdE"
                     /locus_tag="ACHVB7_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR1913682.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15752..17221
                     /gene="rng"
                     /locus_tag="ACHVB7_001671"
     CDS             15752..17221
                     /gene="rng"
                     /locus_tag="ACHVB7_001671"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR1913683.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17289..21089
                     /gene="yhdP"
                     /locus_tag="ACHVB7_001672"
     CDS             17289..21089
                     /gene="yhdP"
                     /locus_tag="ACHVB7_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR1913684.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21245..22690
                     /gene="tldD"
                     /locus_tag="ACHVB7_001673"
     CDS             21245..22690
                     /gene="tldD"
                     /locus_tag="ACHVB7_001673"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR1913685.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22818..23683)
                     /locus_tag="ACHVB7_001674"
                     /pseudo
     CDS             complement(22818..23683)
                     /locus_tag="ACHVB7_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            23866..24069
                     /gene="aaeX"
                     /locus_tag="ACHVB7_001675"
     CDS             23866..24069
                     /gene="aaeX"
                     /locus_tag="ACHVB7_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR1913686.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24077..25009
                     /gene="aaeA"
                     /locus_tag="ACHVB7_001676"
     CDS             24077..25009
                     /gene="aaeA"
                     /locus_tag="ACHVB7_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR1913687.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25015..26982
                     /gene="aaeB"
                     /locus_tag="ACHVB7_001677"
     CDS             25015..26982
                     /gene="aaeB"
                     /locus_tag="ACHVB7_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR1913688.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27074..27346
                     /locus_tag="ACHVB7_001678"
     CDS             27074..27346
                     /locus_tag="ACHVB7_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR1913689.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27402..27665)
                     /gene="yhcN"
                     /locus_tag="ACHVB7_001679"
     CDS             complement(27402..27665)
                     /gene="yhcN"
                     /locus_tag="ACHVB7_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR1913690.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6716 a         6957 c         7379 g         6815 t
ORIGIN      
        1 taaatacaca ttagatgata gattaacctt cgctattttc tcaccctgtg tcgaatatat
       61 ttatttcctg aataattaat catggcaaaa agaaccaaag ccgaagctct gaagacccgg
      121 caagaactga ttgaaactgc catcgcccag tttgcgcaac atggcgtaag caagacgacg
      181 ttcaacgaca ttgccgatgc cgctaacgtt acgcgtggcg ctatctactg gcacttcgag
      241 aacaagaccc aactgtttaa tgagatgtgg ttgcaacagc cttcattgcg ggagttaatc
      301 caggaacact tgacggctgg attagagcat gacccgtttc aacaattgcg tgaaaaattg
      361 attgtcggct tgcaatatat tgccaaaatt ccccgccagc aggcgttgct gaaaatctta
      421 tatcacaaat gtgaatttaa tgatgggatg ctggccgagg gagtgatacg cgaaaagatg
      481 ggctttaatc cgcagactct ccgcgaagta ttgcaggcgt gtcagcaaca aggttgtgta
      541 gcaaataacc tcgatttaga tgttgtgatg attattattg atggtgcctt cagcggaatt
      601 gttcaaaact ggttaatgaa tatggcgggt tatgatcttt ataaacaagc acccgcgctg
      661 gtcgataacg tattaagaat gttcatgcca gatgaaaaca taacgaaatt aattcatcaa
      721 acaaatgaat taagtgtcat gtaaattcac attctgtcct gaaaattcag ttcgtcacag
      781 ttaaccagtt ttagtggata aactgagttc atattacggc atttgtctgt ttctgttgtc
      841 agcagttcaa tccactgcat cagtaaagta tcaaacagaa aaacgaagac agcgaaaacg
      901 acgagccacc aatacttgcg aatcattacc tgagcctgtg catgtttaac caagtctggc
      961 aggaaatata cacgaaatga ataatctgaa aaccaggctt acacttcctt tactttgcaa
     1021 tgagatcggg gtcaacttcg cttaaccggc ttcgttttga caggttgccg gtctttcttt
     1081 ttttcacttt cgccagttct tccgcggagt aatgcgacgc gacatccagc cgtcgaagcc
     1141 ccattttgat gtactcgctg ttgatctcaa taccaatgaa ttttcgtccg ctggcgatgg
     1201 ctacggcacc ggtagtaaag ctaccggcaa acgggtcgag aacgatatcg cctgggttgg
     1261 aagaggcgag aataatgcgt ttcagtaagg cttccggttt ttgcgtcggg tggttctcat
     1321 attcatccat caaataacgc acgcgcggaa aatcccagac gttacccggt actttttgat
     1381 gattgtatgg ctgtggagga tttttgcgat aatcgatcaa cgcgcgctgc gatccggttt
     1441 tggcttctac cagaatagca tcaccgttga atgtgtagtt ctttgcgtct ttcaccatca
     1501 tcaggatggg ttcgtacatg gagccgtagt gttttttcgc ctgtactcca gaactgtcat
     1561 atgaccagac gatgcgactt ttgatggtaa aaagcttgcg gcactggaga tcgataaagg
     1621 gcatgttttc cgtactgttc ataatgtaca tgctgccctg ctttttcaga acgcggtggc
     1681 actctacaat cacttcaaac agccagtcga taaacagatc ttctttccag gcttcgatca
     1741 gaccatcaaa atttttaccg atgttatacg gtgggtcggc aaagatcaga tcgacgcttt
     1801 cagtaggtag ctttttaagt tcggcaaggg catcaccgtg aataatggtc ttagcttcat
     1861 taccaaaccg ggtcggttca catcctgttc tcatggtcac tccctttgtg acacctataa
     1921 aaaaggcgct tccccatgcc gagtagcgcc tttttaatca agcatttagc taacctgaat
     1981 tagttcatgc cgtatttttt caatttttta cgcagcgtac cacggttgat gcccatcatc
     2041 agcgcagcac gggtctggtt accacgggtg tattgcatca ccatgtccaa caggggctgt
     2101 tctacttcag ccagtaccag ctcatagagg tcattcacat cctgaccatt cagttgagca
     2161 aaatagttct tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt tacctgatcc
     2221 tgagagttaa cggtagaaac ggtcagtacg tcagaattta cgcgttgttc gaacatagtt
     2281 ctgtcagctc tttatttctg tttacgcaaa attttcgaag tatgcctcca acgcctccag
     2341 ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat ttggagcgtg
     2401 ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg tacccttttg ccggaccata
     2461 aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg ccaaaggcag
     2521 cgggggcagc aactccccag tgtccagata atgctggatt tcccgaaaga tccagggtct
     2581 tccctgagct gcgcggccta tcatcagggc atccgcccct gtatagtcga gcacagctct
     2641 ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa ctttctgctt
     2701 aactgcccga atactgtcgt actcagcttc tccattgaac aaacaggcgc gtgtacggcc
     2761 atgaatggtc agagcctgaa tgccacagtc ttcagccagt tgggcaatct cttcgcagtt
     2821 acggtgttcc ggtgcccagc cggtgcgaat cttcagggta acaggaacgt ccactgcatt
     2881 gacgacctcg gtaaggatcg atttaacgac atccgggtac tgcaagaggg ctgaacctgc
     2941 gagcttgcga ttcacttttt tagccgggca acccatattg atatcaataa tctgggcacc
     3001 gctttccacg ttaatacgtg ctgcatctgc catttctttc ggatcgctac cagcaatttg
     3061 cacggtgcga ataccgggtt catcaatgtg caccatccgt aaacgagatt tgtcgctttc
     3121 ccaaacctgt gggttagaag acatcatctc ggatactgtc aatccggctc ccatctcgta
     3181 gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga tcaggcgatt
     3241 tctgagctga tattgtccga tgcgcatgag ttaagaaatg accatactgt gactgcaagg
     3301 cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca aactttgacc aatcctctga
     3361 gatggatcaa agaattgcat ttaaaatgag cgtggtgaga taattactca taaaaatcat
     3421 catattagaa aatagtgact aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt
     3481 ttctttatga atgaaaattt tggcacgcaa atttgcgtaa ataaccggca aatttacgtg
     3541 ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct acggcgatgc gaaggttaat
     3601 tcttacgacc ggtaatgcgg caccactctt ctttttccac gaccgggtcc agtgcgaagc
     3661 tgtcgacata agcttcacaa acgctctctg cctggcttgc cagaataccg gaaaggccca
     3721 gcaaaccgcc tgaaaccggc aggacgctga ttaacggtgc cagttcacgt aatgggcctg
     3781 caaggatgtt agcgaccacc acgtcggctt tcatttcttc tggctgatct ttcggtaagt
     3841 agagttccag acggtcagaa acgccattac gttcggcgtt gtcgcggcta gcctgaatcg
     3901 cctgcggatc gatatcaata ccaatggctt ttgctgcacc cagtttcagc gccgcgatag
     3961 ccagaatgcc ggaaccacag ccaaagtcga tgactatttt accggttaaa tcgaggctgt
     4021 cgagccactg caggcacaga gaggtagttg gatgggtacc cgtaccaaac gccagccctg
     4081 gatctaacat cacgttgacg gcgttttcgt ccggcacatc acgccagcta gggcagatcc
     4141 acagtcgttc accaaagcgc atcgggtgga aattatccat ccattcgcgc tcccagtctt
     4201 tatcttccag ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga tggttttcca
     4261 gaatcgccac cacgtcgttc atatcggttt cagcgtcgaa cagaccaatc acatcggtgt
     4321 cgccccacag gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg tgggtatcct
     4381 gaaaagtgat agaaacggca cccgcttcca tcagcgcatc gctaagatct tcagcgttcg
     4441 cgccggtggt gttcagtttc agttggatcc aaggcatggc aaaactcttt atttatcagt
     4501 agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg tttccgacca ggaaagccag
     4561 caaacttagt agtaacgagg gcacgatagg gtggaagccc aggtactgaa tattcagcgt
     4621 cgcgagtacg gcatacagca cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt
     4681 ggcgcgttcc cagtaaagac ccagcaccag cggccacagg aaaacggctt ccagcccacc
     4741 gaaggccagc aaattcagcc agatgatcat ttctggcggc ttccaggcgg caagcagcag
     4801 caacgcgccg agaactaacg taattaccgc cgacatccgc ttcagacgcg tctcgttttg
     4861 catttgatcc ggacggatat tcagatagag atctttaatg atcgtagcgg aactttgtag
     4921 caattgggcg ttaattgtcg acatgatcgc agccataggt gcagccagaa agatcccggc
     4981 agcaaacggt ggcagcactt ttaccattaa cgtcgggatc accagatccg gtacggtgag
     5041 atcggggatc actgcacgac ctaacgctcc ggctaggtgc ataccgaaca tcaggattgc
     5101 gactacaatc gtaccgatga tgatcccccg gtgtaccgct ttgctgtctt tataagagat
     5161 acagcgcacc gcagtatgcg gcaggccaat cacgccaaag cacaccagta cccagaacga
     5221 cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc
     5281 gatggtttgc aaggtctgta ctgcgttgct taagccgcca gcggcatgta ccacgccaat
     5341 aagcagcaca acggtgccaa tcagcatcac aagcccttgc atggtgtcgt tcagcacgct
     5401 ggcgcgaaag ccgccaaagg cggtatacaa cgcaatgctg ataccgaaaa tcagcagccc
     5461 ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc gcaccgccga taaactgcac
     5521 ggtcattgcc ccaacgaacg caaccagcaa actcaaactc gccagccaca ccagaagacg
     5581 actctggtag cgggcaaaca gcatgtcgtt cagcgtcact gcattgtagc ggcgcgcaag
     5641 aatcgcaaac ttcttgccga gaataccgag tgaaagccag actgcaggaa gctgaatcat
     5701 cgccagcaat acccagccca gcccgtattt ataagcagct cctggcccgc cgataaacga
     5761 actggcactg atataggtcg cggtgagcgt catcgccagc acaataccgc ccatagagcg
     5821 gctgccgagg aaatactcat taaggaaggt gccggtgctc cgtttacgca tcgcataaac
     5881 cgagataccg aacaccacca ccagataggc gaccagcggt agaattactt caagctgcat
     5941 cgtcatcctc cagtgggata tcgcgataga taaatttcac catcgcccag cacagtccaa
     6001 taaacagtag cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac
     6061 cgggggcaac gccaggtaag taagcggcta ctaaccaaac tgccagatac aaaagggtca
     6121 gccccagcgc ccagcgcgcc tctttatggg cctgaacaaa acgagtgtcc attttttgtc
     6181 cctgatgggt gaagaaagcg aggattgtac cttatggggg ttgtcgatcc ccagtaataa
     6241 aaaaggccgg aaaatccggc cttttggcgc ttttgcagtc ttatttttcc tgaagaccga
     6301 gttttttctc cagatagtgg atgttagtgc caccatgctg gaagttctcg tcattcatga
     6361 tgcggatctg cagatcaacg ttggttttga taccgtcgat gatcagctcc tgcagcgcat
     6421 tcttcatgcg ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc ttaccgatca
     6481 ttgagtcata gtacggcggt acggtgtagc ccgcgtagat atgagactcc caacgtacgc
     6541 caaaaccgcc aggtgcgtgg aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg
     6601 gatcttcggc gttgatacga cattccaccg catggccgcg aacgtgaact tcttcttgct
     6661 tgatcgacag cggttgaccg gcagcgatac ggagctgttc tttgatcagg tcaacgccag
     6721 tgatcatttc ggtaaccggg tgttctacct gaatacgggt gttcatttcg atgaaataga
     6781 actcgccgtt ttcgaacaga aactcgaaag tacctgcacc gcgatagccg atatcaacac
     6841 acgctttagc gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg cccggtgctg
     6901 gcgcttcttc gaccactttc tggtggcggc gctgcatgga gcagtcacgt tccgccagat
     6961 agatagcgtt gccccgaccg tcagccagta cctgaatctc gacgtggcga ggattttcca
     7021 ggtatttctc catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca
     7081 tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg cgaccaccgc
     7141 cgccgccgga ggctttgata atcaccggat aaccaatgcg tttagcaatg gcacggtttt
     7201 tatccatatc gtcgcccaac gggccgtcag aacccggtac gcaagggacg cccgcttttt
     7261 tcatcgccgc gattgcggat actttgtcgc ccatcaggcg aatggtttct gctttcgggc
     7321 cgatgaagat aaagccggag cgttcaacct gctcggcaaa gttggcgttc tcggagagga
     7381 agccgtaacc cggatggatt gctactgcgc cggtgatttc agcggcgctg atgattgccg
     7441 ggatgttcag ataacttttt actgacggag cagggccaat acagaccgtt tcatctgcca
     7501 gtaatacgtg ttttagatcg cgatccgcgc tggagtgcac agcgacagtc ttgatgccca
     7561 gttctttaca ggcacgaaga atacgcaatg caatctcgcc gcggttggca ataacaattt
     7621 tatccagcat gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt
     7681 tgtccacttt cgaccagaat tgctttcacg gtaccggatt tgtccgcttc gatctggttc
     7741 atcattttca tggcttcaac gatgcacagg gtatcgccca cgttgacttt ctgacccact
     7801 tcgatgaacg cttttgcgtc cgggcttggg gtgcggtaga aagtaccaac catcggggaa
     7861 cgtacgatgt gaccactgat ttccgctgct gctggcgctt ccatggaagg aacggtcgcc
     7921 ggagcggctg cgttagattg agctggctgc tgcatcattg gtgcagcgta agcttgttgc
     7981 atcacaggga aacttgcggc aggagctgca cggctaatgc gtactgactc ttcgccttca
     8041 gaaatttcca gttcggagat gcctgattct tcaaccagct cgatcagttt tttaatctta
     8101 cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac
     8161 cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata gcgataaatt ccagcaggcc
     8221 gggacgccgg tctattttgc cccagagtat caatgttaga cttgacggac attgtgcagc
     8281 gatctgctgc tatcctccgg caaaaaacaa aatatacctt cgttgcgcca atgtcacctt
     8341 ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc acattataac gatttcgtag
     8401 caattggcag ctaaatactg gtctcatcag ggaagataat caacagctaa catgtaaata
     8461 accttcaaca ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag cgccatcatc
     8521 gacggaactt cttataacgt aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg
     8581 gctgcaatga gataatcttc acagaccaca aataatgtag ggatgccagg atcgcgacaa
     8641 gatagacgac attacgcagc ggttgctggg gattacccaa ttttcactgc atcgtctgga
     8701 tagatgtgaa tgctaaagca cgcaaaatca accgactgac aattctcagc attaaatagg
     8761 ttcgagtggt taatttgtag tcaaataaaa tcagattatt caccccatgg taccagaaat
     8821 cagtaacagg ttaagcgtag tgtcgcccag gcaaagcacc ataaacctaa caggcgacgg
     8881 aggcgtatca ataatggctg tttagaggag tatcaatgcg catcaatttg tggtcagtcg
     8941 aaatcatgac tttccagttt ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga
     9001 aagctgtccg gctaaccgct tttcccgtta accgattgaa taatgatgtc aaaatgagtt
     9061 ccctctctta ttattccttg ctaaatggtt agttaacctt caccagcgtg cgaccctgga
     9121 tctggttatt aatgatggcc tcggcaaagt tcggtgcctc tgacagagat atctcttttg
     9181 ccgcctgggt atagaatgat tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc
     9241 ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa acggacatta cgcagaataa
     9301 atggcatgac cgtggttggc agagtaaaac cacccgccag accacaggcc gccacgcagc
     9361 cgccgtaatt catttgcgcc agcacttttg ccagcacttt gtcgccaaca gtgtcaattg
     9421 ccccagccca gacttgtttt tccagaggac gggattcggc aaactcatca cgagggagaa
     9481 tacggctggc acctaaactt ttcagatatt catgggtact ttcgcgaccg gaaacggcaa
     9541 cgacctgata acccaactta tgcagcagcg ccacggcggt actgccgacg ccaccactgg
     9601 cacccgtcac gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca
     9661 cacacagcat ggcggtaaaa ccggcagtac cgataatcat tgctttacgc gcgtccagcc
     9721 cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc agcccacccc
     9781 agtggttttc accaacgccc cagccagtga gtaacacctc ctgaccggca tgaaaacgcg
     9841 gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc ggaaaattac
     9901 ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt
     9961 gaacatcgac cgtgacatcg ccctccggca ggcgactttc gtccagagtc tgtactgatg
    10021 cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc
    10081 atgatggatt ggaaaataat ttctgaagat tatactcgct aatggaaaag caaagccgat
    10141 gaagcgcaat aaattgcgag ataaatctga tttgctagta tgcccgcttc ctcactatcg
    10201 gagttaacac aaggatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg
    10261 ggttaacaat ttttgtgact ttgctgggct gttcgctaag tttctacaac gcaattcagt
    10321 ataagtttag tcatcgcgtt caggcggtgg cgacggcgat tgatacccac cttgtgtcga
    10381 atgacttcag cacattaagg ccacaaatta ccgaattaat gatgtcggca gatatcgttc
    10441 gtgtagacct gctccatggt gataaacagg tttataccct ggccagaaat ggtagttatc
    10501 gtccggttgg ctccagcgat ctgtttcgcg aactgagtgt tccgttgata aagcatccgg
    10561 ggatgtcgct gcgtctggtt tatcaggatc cgatgggcaa ctatttccat tctttaatga
    10621 ctaccgcgcc gctcacgggg gcgattggct ttatcattgt tatgctcttc ctggcggtac
    10681 gctggttaca acggcaactt gccgggcaag aattgctgga aacccgggct actcgtatct
    10741 taaacggtga gcgtggctct aatgtgttgg gaaccatcta tgaatggccg cccagaacca
    10801 gcagtgcgct agatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc
    10861 ttgatacgct gatccgctct tatgccgccc aggacgtgaa aaccggcctc aataaccgac
    10921 tctttttcga taatcagtta gcaacgttac tggaagatca ggagaaagta ggtacccacg
    10981 ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt gagcgacact tgggggcaca
    11041 gccaggttga agaacagttc ttcactctga ctaatctgct gtcgacattt atgatgcgct
    11101 accctggcgc actgctggcg cgttaccacc gcagtgattt tgctgcgcag ttaccgcacc
    11161 ggacgttaaa agaggcagag agcatcgccg gtcagttaat caaagccgtc gataccttgc
    11221 cgaacaataa aatgctcgat cgcgacgata tgatccacat tggtatctgc gcctggcgta
    11281 gtggtcagga taccgagcag gtaatggaac atgcagagtc tgccacgcgt aatgcgggat
    11341 tgcagggcgg caatagctgg gctatttacg atgactcgtt gcctgaaaaa ggacgcggta
    11401 atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc
    11461 aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct
    11521 tcgatggtaa tgaagaggtt agctcggcgg agtatatgcc gatggtcttg cagtttggct
    11581 tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat tccactattg cgttactggc
    11641 cagaggaaaa tctggcgatt caggttaccg ttgagtcgct gattcgcccg cgttttcagc
    11701 gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc ataattattg
    11761 aacttgcaga ggccgatgta ggtcaacata tcagtcgttt acaacctgtt attcgtttag
    11821 tgaatgcttt aggggtacgg gtagccgtca accaggctgg tttgacgctg gtaagcacca
    11881 gttggatcaa agaacttaat gttgagttac tcaagctcca tccggggctg gtcagaaaca
    11941 ttgagaagcg aacggagaac cagctgctgg ttcaaagcct ggtggaagcc tgctccggga
    12001 ccagcaccca cgtttacgcc accggcgtgc gttcgcgaag cgagtggcag accctgattc
    12061 agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc ctcacagcca cttgatacta
    12121 acgtgaaaaa atattcacaa agatactcgg tttaacctgc cgtttaatcc gttttcacgt
    12181 agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc
    12241 acatatgcag atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat
    12301 taagcctttc tggactcagg cagagatttg taacaaagga aacgaactgc actaattttc
    12361 accgtagcag atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga
    12421 ttttcttttc cgccccagct ttcaggatta tcccttagta tgttgaaaaa atttcgtggc
    12481 atgttttcca atgacttgtc cattgacctg ggtactgcga ataccctcat ttatgtaaaa
    12541 ggacaaggca tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt
    12601 tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc agatgctggg ccgtacgccg
    12661 ggcaatattg ctgccattcg cccaatgaaa gacggcgtta tcgccgactt cttcgtgact
    12721 gaaaaaatgc tccagcactt catcaaacaa gtgcacagca acagctttat gcgtccaagc
    12781 ccgcgcgttc tggtttgtgt accggttggc gcgacccagg ttgaacgccg cgcaattcgt
    12841 gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc
    12901 gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt
    12961 ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg tggtttactc ctcttccgtg
    13021 cgcattggtg gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg taattacggt
    13081 tctctgatcg gtgaagccac cgcagaacgt atcaagcacg aaatcggttc ggcttacccg
    13141 ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc
    13201 ggttttaccc tgaactccaa cgaaatcctc gaagcactgc aggaaccgct gaccggtatt
    13261 gtgagcgcgg taatggttgc actggaacag tgcccgccgg aactggcttc cgacatctcc
    13321 gagcgcggca tggtgctcac cggtggtggc gcactgctgc gtaaccttga ccgtttgtta
    13381 atggaagaaa ccggcattcc agtagttgtt gctgaagacc cgctgacctg tgtggcgcgc
    13441 ggtggcggca aagcgctgga aatgatcgac atgcacggcg gcgacctgtt cagcgaagag
    13501 taatcggatg caggcagggg aaatgtctgt ttaccctgcc tggtctgata cgagaatacg
    13561 cataacttat gaagccaatt tttagccgtg gcccgtcgct acagattcgc cttattctgg
    13621 cggtgctggt ggcgctcggc attattattg ccgacagccg cctggggacg ttcagtcaaa
    13681 tccgtactta tatggatacc gccgtcagtc ctttctactt tgtttccaat gctcctcgtg
    13741 aattgctgga tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc
    13801 gggcgttacg tcaggaactg ttgctgaaaa acagtgaact gctgatgctt ggacaataca
    13861 aacaggagaa cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga
    13921 aaatggtgac tcaggttatc tccacggtta acgatcctta tagcgatcaa gttgttatcg
    13981 ataaaggtag cgttaatggc gtttatgaag gccagccggt catcagcgac aaaggtgttg
    14041 ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga
    14101 cccacgcgct gccaatccag gtgctgcgca acgatatccg cgtaattgca gccggtaacg
    14161 gttgtacgga tgatttgcag cttgagcatc tgccagcgaa tacggatatt cgtgttggtg
    14221 atgtgctggt gacctccggt ctgggcggtc gtttcccgga aggctatccg gtcgcggttg
    14281 tctcttccgt aaaactcgat acccagcgcg cttatactgt gattcaggcg cgtccgactg
    14341 caggactgca acgtttgcgt tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta
    14401 acccgatgac gccggaagag gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc
    14461 cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga
    14521 tcgctcagcc gactccgcag caaccgacga caggaaatgc agctactgcg cctgctgcgc
    14581 ccacacagcc tgctgctaat cgctctccac aaagggctac gccgccgcaa agtggtgctc
    14641 aaccgcctgc gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc agggacgttg
    14701 ggtaatctgg ctctctttcc tcattgcgct gttgctgcaa atcatgccct ggccggataa
    14761 cctgattgtt ttccggccaa actgggtgtt actcatcttg ttgtattgga tcctggcctt
    14821 gcctcatcgc gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg atctgatcag
    14881 cggctcgacg cttggcgtac gcgtattggc gatgagcatc attgcttacc tggtggcgct
    14941 gaaataccag cttttccgca acctcgcatt atggcagcag gcgctggtcg tcatgttgct
    15001 ttcgctggtg gtggatatta ttgttttctg ggcagagttt ttagtgatta acgtctcttt
    15061 cagaccggaa gtgttctgga gtagtgtagt caatggggtg ctctggccgt ggattttctt
    15121 gctgatgcgt aaagtccgtc agcagtttgc agtgcaataa aggtttctat gacttctctg
    15181 tatttagctt ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg tgtgaccttt
    15241 gaacgtattg ttacgggcat tgaggagcag cgtcagccgc aggagagcgc gcagcagtat
    15301 gttgtgcgtc tggcgcgcga gaaagcacag gcgggtgtcg cgcaaacggc gcaggatctc
    15361 ccggtgctgg gtgcggatac tatcgttatc ctgaatggag aagtgctgga gaaaccgcgc
    15421 gacgcagagc atgcggcgca gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg
    15481 acggcagtgg cgttggccga cagccagcac attctcgatt gcctggtggt caccgatgtg
    15541 actttcagaa cgttaacaga cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta
    15601 gataaagcag gtgcatacgg tattcagggg ctgggtggct gttttgtcag gaagataaat
    15661 ggcagctatc acgccgtagt cggcttacct ctggttgaaa cgtatgaatt attaagtaat
    15721 tttaacgcac tgcgtgagaa aagggataaa catgacggct gaattgttag taaacgtaac
    15781 gccttcggaa acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa ttcatattga
    15841 acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact
    15901 tccgggtatg caggcggctt ttgtagatat tgggctggat aaagccgcgt ttcttcatgc
    15961 atccgatatc atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt
    16021 gcgcgacatc tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga
    16081 tccgcttggc actaaaggtg cgcgcctgac caccgatatc acgctgcctt ctcgctatct
    16141 ggtctttatg ccaggggctt ctcacgttgg ggtttcccaa cgtattgaaa gcgaatcaga
    16201 acgtgaacgc ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg ggtttatcat
    16261 ccgtaccgca gcggaagggg ttggcgaggc tgaactggcc tccgatgccg cttatctgaa
    16321 acgcgtctgg accaaagtta tggagcgtaa aaagcgcccg cagacccgtt atcagctgta
    16381 cggcgaactg gcgctggctc agcgtgttct gcgtgatttc gccgacgccg aactggaccg
    16441 cattcgcgtt gactcacgcc tgacttacga agcattactt gagttcacct cggagtacat
    16501 tcccgagatg accagcaagc tggagcatta caccggacgc cagccgattt tcgatctctt
    16561 tgatgtcgaa aacgaaatcc agcgagcgct ggaacgcaaa gtagaactga aatccggtgg
    16621 ttatctcatt atcgaccaga ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt
    16681 tgtcggtcat cgcaatctgg acgacaccat tttcaatacc aatattgaag cgacgcaggc
    16741 tatcgctcgc cagttacggt tgcgtaatct gggcgggatt atcattattg atttcatcga
    16801 tatgaataat gaagatcacc gccgccgcgt gctgcactcg ctggagcagg cgttgagcaa
    16861 agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg agatgacgcg
    16921 taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg
    16981 tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg agattgttcg
    17041 tgtccaccat gcttacgact ccgaccgttt cctggtctat gcttctccgg cagtagccga
    17101 agccttgaaa ggcgaagagt cacactcgct ggcggaagtg gaaattttcg ttggcaaaca
    17161 ggttaaagta caaattgaac cgctctataa ccaggagcag tttgacgtcg taatgatgta
    17221 aacagatgct gggccgccat ccggcaaagg gtttttgagt cacattttta gcagacaagg
    17281 agtgacgggt gaggcgattg ccggggattt tactgcttac tggagccgcg ctcgttgtga
    17341 tcgctgccct gctggttagc ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc
    17401 cggaaatcct caacaaaata gaatccgcga ctggcatgcc ggtagaagcc agtcagctct
    17461 cagccagctg gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa
    17521 aagatggcgg cgaattttcg gttaaacgcg ttactctggc gctggatgtc tggcagagcc
    17581 tgttacatat gcgctggcag tttcgcgacc tcactttctg gcagctgcgc tttcgcacca
    17641 acactcctat caccagcggt ggtagcgatg acagtctgga agccagtcac atcagcgatc
    17701 tgtttcttcg tcaatttgac catttcgatc tccgcgacag tgaagtcagt ttcctgacgc
    17761 catccggtca gcgtgccgag ctggcgatcc cacaactcac ctggctgaac gatccacgtc
    17821 gacaccgtgc ggaaggcctg gtaagcctct ccagccttac cggacagcac ggcgtgatgc
    17881 aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag caatggtcgc gtctggctac
    17941 aggcggatga catcgacctg aagccgtggc tcggtaaatg gatgcaggac aatattgctc
    18001 tggaaacggc acagttctcc cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg
    18061 gcggtgacgt ctggctgaaa cagggcggtg ccagctggtt gggcgagaag caaacgcata
    18121 cgctgtcggt ggataatctg accgcgcata ttacgcgtga aaatccgggc tggcagttct
    18181 ctattcccga tacacggatc acgatggacg gcaaaccctg gccgagcgga gcattgacgc
    18241 tggcctggat accggaacag gacgttggcg gcaaagacaa taaacgcagt gacgaactcc
    18301 ggattcgcgc cagtaatctg gagctggcag gcctggaggg catacgcccg ctggccgcga
    18361 aactttcacc tgcactgggt gatgtttggc gctccacaca accgagcggc aagattaaca
    18421 ctctggcgct ggatatcccg cttcaggcgg cagacaagac ccgttttcag gcatcgtgga
    18481 gcgatctggc ctggaagcaa tggaaattat taccgggtgc ggaacacttc tccgggacgc
    18541 tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt
    18601 acgaaacggt attccgtgcg ccactggaaa tcgccgacgg ccaggcaact ataagctggc
    18661 tgaacaatga caaaggtttc cagctggatg ggcgtaatat tgacgttaaa gccaaagccg
    18721 tccatgcgcg cggcggtttt cgttacctgc aacctgctaa cgatgaaccc tggctgggta
    18781 ttctggctgg catcagtacc gatgatggtt cacaagcctg gcactatttc ccggaaaact
    18841 tgatgggtaa agacctggtt gattacttaa gtggcgcgat tcagggcggt gaagcggata
    18901 acgcgacgct ggtttatggt ggcaatccgc aactcttccc ctataaacac aacgaaggtc
    18961 agtttgaagt gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg gactggcctg
    19021 cattaactaa ccttgatatt gaactggact ttattaacga cggtttatgg atgaaaaccg
    19081 atggcgttaa tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc cctgactact
    19141 cgaaagaaaa actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt
    19201 actttgatga gacaccgctg aaagattctc tgggtgcgac cctgcaagaa ctccagctcg
    19261 acggcgatgt gaatgctcgc ttacatcttg atatcccgct gaacggcgag ctggtaaccg
    19321 cgaaaggtga agtgacgctg cgtaataaca gtctgtttat caaaccactc gacagcaccc
    19381 tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt gaaccactga
    19441 cagcaagctg gtttaatcag ccgttgaacg tggatttttc caccaaagaa ggggcaaaag
    19501 cctaccaggt tgcggtgaat ctcaacggta actggcaacc ggcgaaaacc ggcgttctgc
    19561 ctgaagcggt gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg
    19621 atctgcctta tcatgctggc gcgacctata acgtagagct gaacggcgat ctgaataatg
    19681 tgagtagtca cttaccttca ccgttagcca aacctgcggg tgaaccacta gcggtaaacg
    19741 ttaaggttga tggcaatctc aacagctttg aattaaccgg acaggctggt gcggataacc
    19801 atttcaatag ccgctggttg cttggtcaaa agctgacgct cgatcgtgct atttgggcgg
    19861 cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg cgttgaactc aatatgccgc
    19921 cgatgaatgg tgccgagtgg ctggccctgt tccagaaagg cgctgcggag agtgtcggtg
    19981 gtgcagcgag tttcccacaa tacataacgt tacgtacgcc tatgttgtca ctgggaaatc
    20041 agcaatggaa taacctgagt attgtttcgc aaccgacggc aaatggcacc ctggttgagg
    20101 cgcaagggcg tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg tggctggcga
    20161 atatcaaata tctttattac aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt
    20221 catcaccttt cccgacaacg gagcgcatta acttccgtgg ctggccggac gcacaaatac
    20281 gatgcacaga gtgctggttc tgggggcaaa aattcggtcg cattgacagt gatatcacca
    20341 tttctggcga tacgttaacg ctgaccaatg gactgattga tactggtttc tcgcggctta
    20401 ctgccgatgg tgaatgggtt aataatccgg ggaatgaacg tacctcgctg aaaggaaaac
    20461 tgcgcgggca gaaaattgat gccgccgcag aattttttgg tgtcacgacg cccatacgcc
    20521 agtcgtcatt taatgtggat tacgatttac actggcgcaa agcaccctgg cagccagatg
    20581 aagcgacgtt gaatggcatc attcatactc aactgggtaa aggcgaaatt accgaaatca
    20641 ataccggaca tgccgggcaa ttgctgcgct tattgagcgt agatgccctg atgcgtaagc
    20701 tgcgttttga tttcagagac acttttggcg aagggttcta ttttgactcc attcgcagca
    20761 ccgcgtggat taaagacggg gttatgcaca ccgacgacac gctggtggat ggcctggagg
    20821 cggatatcgc catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg
    20881 ttgtcgcacc agagatttct gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca
    20941 ttgttggcgc ggcagttttt gccgctagta aagtgctggg gccgctgtgg agcaaagtct
    21001 ccattttgcg ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc
    21061 gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc
    21121 atatgataat cgttgccaaa ggccaacgag ccagaacata accgtaggcc ggatagggcg
    21181 ttcatgccgc atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa
    21241 aacgatgagt ctgaacctgg taagtgaaca attgctagcg gcgaacagcc tgaaacatca
    21301 ggacttgttc gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta
    21361 ttttcagtcg agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc
    21421 ttacaacatc gatcagggcg ttggcgtgcg tgcaatcagc ggtgaaaaaa ccggatttgc
    21481 ttacgctgac caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat
    21541 cgtccgtgat agtggtgatg gcaaagtaca gacgctgggc gcggtagagc atagcccgtt
    21601 gtatacctcg gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg
    21661 tcgcgtcgat aaggttgctc gcgaagcgga caagcgcgta caggaagtga ctgccagcct
    21721 cagtggtgtc tatgaattaa ttctggttgc ggccaccgac ggcacgctag cggcggatgt
    21781 ccgtccgctg gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg
    21841 cggtgccagt ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg atcttgacgg
    21901 cgaagtccgt gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc
    21961 tgccgttgct gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt
    22021 gctgttgcat gaagcggtag gtcacggtct ggaaggcgac ttcaaccgcc gcggcacttc
    22081 agtatttagt gggcaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga
    22141 cggcacgatg gtcgatcgcc gtggttcggt ggcgattgat gacgaaggta cgccaggcca
    22201 gtacaacgtg ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc
    22261 gcgtttgatg gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc
    22321 catgccgcgt atgactaaca cctatatgct gccgggtaaa tcgaccccgc aggaaattat
    22381 tgaatccgtt gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatatcac
    22441 ctccggcaaa ttcgttttct ccacttcaga agcatatctg attgaaaacg gtaaagtaac
    22501 gaagccggtg aaaggcgcaa cgttgattgg ttccggtatc gaaaccatgc agcagatttc
    22561 gatggttggc aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca
    22621 aagtttgccg gttggcgtgg gccagccaac gctgaaagtt gataacctga ctgttggcgg
    22681 tactgcgtaa taatttcatt attttcagaa ggataattaa tctttctacg tgcacgaacg
    22741 gtcccctcgc tcctctgggg ttagggtgag gggaacccgt tggcacaggt ttgtagaacg
    22801 taacagtaca atatgaatta cttctctttc ccgcgcccgt gcatctcctg aaacaattta
    22861 ccgacctcaa caaaataatc cgtcagcgag ttgatcacga cctgtacctt cagcggcagc
    22921 ttatcttttt cggtatataa cgcataaacc gggcgtggat ctgactgata acgcggcagc
    22981 aggatctcca gctcaccgcg attgatctcg ttgatcaccc acatcagcgg cacgtaggcg
    23041 atcccggcac ccgccgtcag ccagcgcacc agcgtcatcg gatcattagt cacaaatctt
    23101 ccttgtggga tcaggcgagt cgagatccct tccggtgcga tcagttcaaa ttcattgtcg
    23161 ggccgcacgc tgtattcaag ccatgaatga ctactcaaat cggcgggttt ttccggtatg
    23221 ccgtattgtg tgagatagct tttcgcggcg cacaccacca tcggcatcgc gcccagacgg
    23281 cgggaaaaca ggctggaatt ccggtaacca gattgacgct caaacctggg tattctttca
    23341 gcatttttgc tgtcagcccg gcgagaacat tttgtgccat agttgaagaa cagccaatgc
    23401 gcagcgtccc gatgggggtg ttattgaagg catacagttg ctcatgaaca tcctgcactt
    23461 catgaagcat ccgacggcag ccctggtagt aaattctgcc ggcttcggtc aggccaatgc
    23521 tgcgtgtgct acggtttaac agctttacct gcaactcatc ttccagtttt gacaccgtct
    23581 gactgatgga cgaaacgctc atctgtagct gtctggcggc ggcggtaaaa gagccaaatt
    23641 caactacttt ggcaaacacc gacatgcgtt ttagtcgttc cattattcac tctgacttaa
    23701 aagtgattta gatcacataa tatagataac agcataacag ttacgctaat atattaaata
    23761 tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc ggcgacagat
    23821 gctgaaaata ataacgcctg ctctctcttt acaaccaagg tcaacatgag tctgtttccc
    23881 gttatcgtgg tgtttggact gtccttccca ccgatatttt ttgaattgct tttatcactg
    23941 gcgattttct ggctggtgcg ccgggtactt gtgccaacag gtatctacga ctttgtctgg
    24001 catccggcgt tgttcaacac cgcgctctat tgctgcttgt tttatttgat atcgcgactg
    24061 ttcgtttgag gttgaagtga aaacactaat aagaaaattc tcccgtacgg ccatcacggt
    24121 cgtattagtc attctagcct tcatcgcaat ttttaatgcc tgggtctatt acaccgaatc
    24181 cccctggacg cgtgacgcgc gctttagcgc tgacgtcgtt gcgatcgcgc cggacgtttc
    24241 tggactcatt acccaggtga atgttcatga taaccagctg gtgaaaaaag gacagatact
    24301 gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag gaagcgcaag ccgatgttgc
    24361 ttattatcag gtactggcac aggagaaacg ccaggaggcc ggacgtcgta accgtctcgg
    24421 tgtgcaggcg atgtctcgcg aagagatcga ccaggccaac aacgtactac aaacggttct
    24481 gcatcagtta gcgaaagcgc aggcgacccg cgatctggca aaactggatc ttgaacgcac
    24541 ggtgatccgc gcgccagcag atggctgggt gaccaacctc aacgtctata ccggtgagtt
    24601 tattactcga ggatcaacgg cggttgcgct ggtgaaacag aactccttct atgtactggc
    24661 ctatatggaa gaaactaagc tggaaggggg gcgtccgggg tatcgtgcag agatcacgcc
    24721 gcttggcagt aacaaagtgc tgaaagggac tgttgatagt gttgccgcag gggtcaccaa
    24781 cgccagcagc acgcgtgacg acaaagggat ggcgactata gactctaatc ttgaatgggt
    24841 gcgtcttgcg caacgtgttc cggttcgtat tcgtctcgac aaccagcaag agaacatctg
    24901 gcctgcgggc accactgcta cagtggtggt cactggcaaa caagatcgcg acgaaagcca
    24961 ggattcgttc ttccgtaaaa tggcccatcg cctgcgtgag tttggttaat cacgatgggt
    25021 attttctcca ttgctaacca acatattcgc tttgcggtaa aactggcgac cgccattgta
    25081 ctggcgctgt ttgttggctt tcacttccag ctggaaacgc cacgctgggc ggtactgaca
    25141 gcggcgattg ttgccgccgg tccggccttt gctgcgggag gtgaaccgta ttctggcgct
    25201 attcgctatc gtggcttttt gcgcatcatc ggcacattta ttggctgtat tgccggactg
    25261 gtgatcatca ttgcgatgat ccgcgcacca ttattgatga ttctggtgtg ctgtatctgg
    25321 gccggttttt gtacctggat atcctcgctg gtacgaatag aaaactcgta cgcgtggggg
    25381 ctggccggtt ataccgcgct gatcattgtg atcaccattc agccggaacc attgcttacg
    25441 ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg gtattgtgtg tgcgattatg
    25501 gcggatttgc tcttttctcc gcgatcgatc aaacaagaag tagatcgaga gctggaaagt
    25561 ttgctggtcg cgcaatatca attaatgcaa ctctgtatca agcatggcga tggtgaagtt
    25621 gtcgataaag cctggggcga cctggtgcga cgcaccacgg cactacaagg catgcgcagc
    25681 aacctgaata tggaatcttc ccgctgggcg cgggccaatc gacgtttaaa agcgatcaat
    25741 acgctatcgc tgacgctgat tacccaatcc tgcgaaactt atcttattca gaatacgcgc
    25801 ccggaattga tcactgatac tttccgcgaa ttttttgaca cgccggtaga aaccgcgcag
    25861 gacgtccaca agcagctcaa acgcctgcgg agagttatcg cctggaccgg ggaacgggaa
    25921 acgcctgtca ccatttatag ctgggtcgcg gcggcaacgc gttatcagct tctcaagcgc
    25981 ggcgttatca gtaacacaaa aatcaacgcc accgaagaag agatcctgca aggcgaaccg
    26041 gaagtaaaag tagagtcagc cgaacgtcat catgcaatgg ttaacttctg gcgaaccaca
    26101 ctttcctgca ttctgggcac gcttttctgg ctgtggacgg gctggacttc cggcagtggt
    26161 gcaatggtga tgattgcggt cgtgacgtca ctggcaatgc gtttgccgaa tccacgcatg
    26221 gtggcgatcg actttatcta tgggacgctg gccgcgctgc cgttagggct gctctacttt
    26281 ttggtgatta tccctaatac ccaacagagc atgttgctgc tgtgtattag cctggcagtg
    26341 ctgggattct tcctcggtat agaagtacag aaacggcgac tgggctcgat gggggcactg
    26401 gccagcacca taaatattat cgtgctggat aacccgatga ctttccattt cagtcagttt
    26461 ctcgacagcg cattagggca aatcgtcggc tgtgtgctcg cgttcaccgt tattttgctg
    26521 gtgcgggata aatcgcgcga caggactgga cgtgtactgc ttaatcagtt tgtttttgcc
    26581 gctgtttccg cgatgactac caatgtggca cgtcgtaaag agaaccacct cccggcactt
    26641 tatcagcagc tgtttttgct gatgaataag ttcccagggg atttgccgaa atttcgcctg
    26701 gcgctgacga tgattatcgc gcaccagcgc ctgcgtgatg cgccgatccc ggttaacgag
    26761 gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag accatgtgat atctgcccgt
    26821 agcgatgata aacgtcgtcg gtactttggc cagttgctgg aagaactgga aatctaccag
    26881 gaaaagctac gcatctggca agcgccaccg caggtgacgg aaccggttca tcggctggcg
    26941 gggatgctcc ataagtatca acatgcgttg accgatagtt aagtcaaaac cgacgccaaa
    27001 agcgtcggtt ttttcatggc tatacttagc gatgcacggc agaactcgcc gcgaaacgtg
    27061 acggtggcaa cagatgaata tttatacctt tgattttgat gagattgaga gtcaggagga
    27121 tttttatcgt gactttagcc aaacctttgg tctggctaaa gataaggtac gcgatctcga
    27181 ctcactatgg gatgtgttaa tgaacgatgt cctgccgcta ccacttgaga ttgaatttgt
    27241 tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta atattgctgt ttgatgaggc
    27301 agaggaagag ctggaagggc atttgcgttt taatgttcgt cattagcgca aaaaaaagcc
    27361 cccgaaccgg gggcaatatc gtcggacaag acgatgaggg tttatttgta cagttcagcc
    27421 gtagcgtgcc aggtgtcacc gctacgagct tcagtaatct ggtaggccgt tgcgcctttc
    27481 tcttccgctt ttttgttcag catttcacgc atatccattg gcgaagacgc cacaccactt
    27541 acggatacgg tcccgattgc ttcacgattt tgtgcttgtg cagtatcaat ggagtcggca
    27601 gcgaatgcac cgaaagagag aacagaaagt acgcttaatg cagctacagt ggttttgatt
    27661 ttcatgattt ttacctcgac ataatctttt agctgggcct ttgtttcgtg atccatatca
    27721 caaaatcaag catacactaa ttattgaact aattaatacc acactaactg tttcagttga
    27781 tactaagtgt taaaaatgtg aattttaata acaaaaaaga attaaaaaca cggttattta
    27841 ttgttaattc tagtattttc aattaga
//
LOCUS       DAZPJR010000043        27105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPJR010000043 DAZPJR010000000
VERSION     DAZPJR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.044"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..921
                     /locus_tag="ACHVB7_001680"
     CDS             <1..921
                     /locus_tag="ACHVB7_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1913691.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHVB7_001681"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHVB7_001681"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR1913692.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHVB7_001682"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHVB7_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR1913693.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="ACHVB7_001683"
     CDS             3882..4277
                     /locus_tag="ACHVB7_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR1913694.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="ACHVB7_001684"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="ACHVB7_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR1913695.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="dolP"
                     /locus_tag="ACHVB7_001685"
     CDS             4897..5472
                     /gene="dolP"
                     /locus_tag="ACHVB7_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR1913696.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="ACHVB7_001686"
     CDS             complement(5586..6626)
                     /locus_tag="ACHVB7_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1913697.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="ACHVB7_001687"
     CDS             complement(6699..7334)
                     /locus_tag="ACHVB7_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR1913698.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHVB7_001688"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHVB7_001688"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR1913699.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="ACHVB7_001689"
     CDS             complement(7960..8403)
                     /locus_tag="ACHVB7_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR1913700.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHVB7_001690"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHVB7_001690"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR1913701.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="ACHVB7_001691"
     CDS             complement(8743..9246)
                     /locus_tag="ACHVB7_001691"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1913702.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="ACHVB7_001692"
     CDS             complement(9240..9764)
                     /locus_tag="ACHVB7_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR1913703.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..10968
                     /locus_tag="ACHVB7_001693"
     CDS             9973..10968
                     /locus_tag="ACHVB7_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR1913704.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10977..11855
                     /locus_tag="ACHVB7_001694"
     CDS             10977..11855
                     /locus_tag="ACHVB7_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR1913705.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12061..13068
                     /locus_tag="ACHVB7_001695"
     CDS             12061..13068
                     /locus_tag="ACHVB7_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR1913706.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHVB7_001696"
     CDS             complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHVB7_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR1913707.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHVB7_001697"
     CDS             complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHVB7_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR1913708.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHVB7_001698"
     CDS             complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHVB7_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR1913709.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHVB7_001699"
     CDS             complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHVB7_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR1913710.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHVB7_001700"
     CDS             complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHVB7_001700"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR1913711.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHVB7_001701"
     CDS             complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHVB7_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR1913712.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHVB7_001702"
     CDS             complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHVB7_001702"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR1913713.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHVB7_001703"
     CDS             complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHVB7_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR1913714.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHVB7_001704"
     CDS             complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHVB7_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR1913715.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHVB7_001705"
     CDS             complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHVB7_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR1913716.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHVB7_001706"
     CDS             complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHVB7_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR1913717.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26826..26902)
                     /locus_tag="ACHVB7_001707"
     tRNA            complement(26826..26902)
                     /locus_tag="ACHVB7_001707"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26866..26868),aa:Met,seq:cat)
BASE COUNT         6421 a         7516 c         6811 g         6357 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    10201 tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    10261 ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    10321 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgatgttgcc
    10381 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggtcaca
    10441 cctgtcccgc tggaagtctt tgcttttggc agcctgtgca ttatgtcgga aggtcgttgc
    10501 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg ttctccggcc
    10561 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    10621 atcgaccgtt atcaggatgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat
    10681 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    10741 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    10801 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    10861 gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    10921 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    10981 aatattcctt agggccagtg ctgtggtact ggccaaaaga ggcgctggaa gaattttatc
    11041 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    11101 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagcggga
    11161 agcagatagt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    11221 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    11281 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    11341 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    11401 cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gttggggatt cgcaaccagt
    11461 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    11521 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    11581 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    11641 ccatgagcgg ctacgtttac aacctcggta acgagctgac atccatgcag ggattggttg
    11701 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    11761 ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa cagcgactgt aacggctact
    11821 ggcggcggct ggctgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    11881 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    11941 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    12001 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    12061 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    12121 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    12181 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    12241 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    12301 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    12361 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    12421 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    12481 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    12541 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    12601 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    12661 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    12721 atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt tctatttacc
    12781 tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccggacaact gccgccgccg
    12841 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    12901 agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg gcttgcagtc gatcctgcgc
    12961 gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca ggcgcgcctg
    13021 cattcgtttg aactggcgat ggatgtgaag gaagagttgt tgggatagtg tgtcttaacg
    13081 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    13141 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    13201 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa acaccagaat
    13261 cagcgtaatc atcggcttgc cgccccagac acggaatttc gggctgccaa agcgtttacg
    13321 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    13381 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    13441 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    13501 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    13561 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    13621 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    13681 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    13741 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac
    13801 gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcagca aatacggcgc
    13861 ataagatgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    13921 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agtacaatcg ccgtcatgcg
    13981 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    14041 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    14101 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    14161 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    14221 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    14281 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    14341 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    14401 tgacgtttgg gtggtggtta gtgttgccat gagagcttct ctccagtgaa aaatagtgcg
    14461 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    14521 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    14581 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    14641 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    14701 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    14761 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    14821 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    14881 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctac tgatgtcgcc
    14941 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    15001 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    15061 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    15121 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    15181 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    15241 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    15301 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    15361 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    15421 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    15481 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    15541 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    15601 caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    15661 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    15721 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    15781 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    15841 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    15901 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    15961 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    16021 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    16081 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    16141 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    16201 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    16261 ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc cgctccccgt
    16321 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    16381 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    16441 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    16501 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    16561 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    16621 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    16681 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    16741 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    16801 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    16861 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccaag
    16921 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    16981 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    17041 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    17101 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    17161 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    17221 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    17281 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    17341 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    17401 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    17461 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    17521 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    17581 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    17641 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    17701 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc tagaactttc
    17761 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    17821 atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    17881 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    17941 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    18001 gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc acggataaca
    18061 gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat ggtatggata
    18121 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    18181 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    18241 ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga acctgcaact
    18301 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    18361 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gattggcaca
    18421 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    18481 gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat atccggcatg
    18541 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    18601 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggta
    18661 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt
    18721 gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc
    18781 agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc
    18841 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    18901 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    18961 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    19021 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    19081 tgccagtccc aacgcggttt accggcttct ttcactagtt cattgatgtt ctgaataaca
    19141 acctgctgtt gttcatgacc gaacactact gcgcccagca tctgatcttc gctcagcagt
    19201 tcagcttcag attcaaccat cagtacagcg gcttcagtac cggcaacaac cagatccagt
    19261 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    19321 acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag tgctgcggaa
    19381 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    19441 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    19501 agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa gaagctaccc
    19561 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    19621 tgacctggtt tggctttttt ctgtccaaca acggtaacga ataccgcggt gtcatccatg
    19681 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    19741 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    19801 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    19861 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    19921 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    19981 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    20041 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    20101 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    20161 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    20221 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    20281 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    20341 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    20401 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    20461 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    20521 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    20581 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    20641 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    20701 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    20761 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    20821 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    20881 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    20941 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    21001 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    21061 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    21121 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    21181 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgcctgttgc
    21241 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    21301 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    21361 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    21421 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    21481 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    21541 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    21601 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    21661 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    21721 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    21781 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    21841 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc
    21901 aatccatgcc ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    21961 gatggtacgt tggatctcga tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    22021 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    22081 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    22141 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatagcacc
    22201 aaatttcggc gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    22261 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    22321 ggagtagtaa cgcagatcca ggctttccgc ttcaatcact ttacgtgcag aggcatcagc
    22381 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    22441 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    22501 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatgtt
    22561 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    22621 ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc
    22681 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc
    22741 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    22801 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    22861 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    22921 aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag
    22981 caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa
    23041 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    23101 acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg
    23161 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    23221 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    23281 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    23341 accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg aacgaatgta
    23401 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    23461 cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    23521 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    23581 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    23641 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    23701 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    23761 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    23821 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc
    23881 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    23941 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    24001 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gatgcgcttc
    24061 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    24121 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    24181 tttcgcttgt tcagcagctt cacgcttagc ttgttctgca gcctcacgtt cagctttttg
    24241 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    24301 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24361 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24421 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    24481 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    24541 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    24601 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    24661 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggcct tttcgtcggt
    24721 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    24781 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    24841 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    24901 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    24961 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    25021 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25081 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    25141 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25201 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25261 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    25321 gatatcgata cgctcgccac ccagttcagt agacaccgcc tgaacacgcg cgccacgcat
    25381 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25441 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    25501 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    25561 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    25621 gaagttttca cgcggcagca tatcttcacg caggatcacg gcttcagcgt tgttgcccag
    25681 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    25741 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    25801 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    25861 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    25921 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    25981 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    26041 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26101 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26161 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26221 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26281 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26341 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26401 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    26461 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    26521 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    26581 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    26641 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    26701 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    26761 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    26821 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    26881 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa
    26941 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    27001 gtgatccacg ccacattttg tcaacgttta ttgctaatca tgtgaatgaa tatccagttc
    27061 actttcattt gttgaatact tttgccttct cctgctcccc cttaa
//
LOCUS       DAZPJR010000044        26843 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPJR010000044 DAZPJR010000000
VERSION     DAZPJR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.045"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHVB7_001708"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHVB7_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR1913718.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHVB7_001709"
     CDS             567..650
                     /locus_tag="ACHVB7_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913719.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHVB7_001710"
     CDS             750..1571
                     /locus_tag="ACHVB7_001710"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR1913720.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHVB7_001711"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHVB7_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR1913721.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHVB7_001712"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHVB7_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR1913722.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHVB7_001713"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHVB7_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR1913723.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHVB7_001714"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHVB7_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR1913724.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHVB7_001715"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHVB7_001715"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR1913725.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHVB7_001716"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHVB7_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR1913726.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHVB7_001717"
     CDS             8347..9729
                     /locus_tag="ACHVB7_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1913727.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHVB7_001718"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHVB7_001718"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR1913728.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHVB7_001719"
     CDS             complement(10485..10820)
                     /locus_tag="ACHVB7_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR1913729.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHVB7_001720"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHVB7_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR1913730.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHVB7_001721"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHVB7_001721"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR1913731.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHVB7_001722"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHVB7_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR1913732.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHVB7_001723"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHVB7_001723"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR1913733.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="ACHVB7_001724"
     CDS             complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="ACHVB7_001724"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1913734.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17366..18541
                     /gene="manA"
                     /locus_tag="ACHVB7_001725"
     CDS             17366..18541
                     /gene="manA"
                     /locus_tag="ACHVB7_001725"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR1913735.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18642..20150
                     /locus_tag="ACHVB7_001726"
     CDS             18642..20150
                     /locus_tag="ACHVB7_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1913736.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="ACHVB7_001727"
     CDS             complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="ACHVB7_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR1913737.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="ACHVB7_001728"
     CDS             complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="ACHVB7_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR1913738.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGFCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="ACHVB7_001729"
     CDS             complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="ACHVB7_001729"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR1913739.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="ACHVB7_001730"
     CDS             complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="ACHVB7_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR1913740.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="ACHVB7_001731"
     CDS             complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="ACHVB7_001731"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR1913741.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6737 a         6950 c         6455 g         6701 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgat ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tagctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggccgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatga aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc ggctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgagtgc
    15541 actgtgtgag ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg
    15601 ccgggaaatc ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa
    15661 cacattccag gctcttaata cttccctgcg ccaatacagc ggccggacca ccgatactgc
    15721 caaggtagaa gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt
    15781 tcgccagcat gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc
    15841 gtccggcggt cgttgggcca agagaaccgg aggcataacc ttccggcgtt ttggccggac
    15901 ccgcgtagta aatcggatga tctttaatgt actgcggcag cccttcaccg ttatccatcc
    15961 gctctttcag tttggcgtga gcaatatcac gaccgacgat aatcgtgccg ttaagcgata
    16021 agcgtgtaga aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa
    16081 ggtcaacgcg caccgcttcg ccttctcccg ctttacgcag ctcttcaggg atatatttac
    16141 ctggattatg ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat
    16201 tacggtcagc agagcaggaa acacccatac caaccggaca ggatgcgccg tgacgtggca
    16261 ggcgaatcac gcgtatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccga
    16321 gattttgcgc ttcgatcagc aattcttttt ccagttccac atcgcggaat gcctgaccgt
    16381 gctcattccc ttccgttggc agttcgtcat agtatttagc ggaagccagt ttcactgttt
    16441 taaggttagt ttctgcagaa gttccaccaa ttacgaacgc aatatgatac ggaggacagg
    16501 ccgccgtacc cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtta
    16561 gtaacgcttt ggtttcctga tagagatacg tcttgtttgc cgaaccacca cctttggcga
    16621 tacagaggaa tttgtactcg tcgccatcaa cggcataaag atcgatctgc gctggcagat
    16681 tggtgccggt attcacctct ttatacatat ccagcggcgc gttttgcgag tagcgcaaat
    16741 tatcttcgat ataagtgtta tagacaccgc gcgccagcgc cgcttcatca cagccaccgg
    16801 tccatacacg ctgccctttt ttaccaacaa taatcgcagt gccggtatcc tgacaggttg
    16861 gcagaacacc tttcgccgcg atgtcggagt tgcgcaggaa ttgcagcgcc acatatttat
    16921 cattttcgct ggcttccggg tcacgcagaa tgtcggccac ctgttgttgg tgagccggac
    16981 gcagcataaa tgacgcatca tgaaacgcct gacgcgccaa cagagttaac gcttcgggtg
    17041 cgactttcaa aatctcctgc ccttcaaatt cagatacgct aacgtgttcg ctggttagca
    17101 ggtaatactc agtatcatct tttttgagtg gaaaaggagc ctgataatga aagggtttgt
    17161 ttgacattgt tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg
    17221 ccctgttaaa agcgagtaac aatatcctac acactttttt aacaaaaact gagactagta
    17281 cgactttttg cggctccagg ttacttcccg taggattctt gctttaatag tgggattaat
    17341 ttccacatta aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc
    17401 ctggggcagc aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc
    17461 gatggccgag ctgtggatgg gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc
    17521 cggagatatc gtttcactgc gtgatgtgat tgagagtgat aaatcgactc tgctcggaga
    17581 ggccgttgcc aaacgctttg gcgaactgcc tttcctgttc aaagtattat gcgcagcaca
    17641 gccgctctcc attcaggttc atccaaacaa acgcaattct gaaatcggtt ttgccaaaga
    17701 aaatgccgca ggtatcccga tggatgccgc cgagcgtaac tataaagatc ctaaccacaa
    17761 gccggagctg gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc gtgagttttc
    17821 cgagattgtc tccctactcc agccagtcgc aggtgcacat ccggcaattg ctcacttttt
    17881 acaacagcct aatgccgaac gtttaagcga actgttcgcc agcctgttga atatgcaggg
    17941 tgaagaaaaa tcccgcgcgc tggcgatttt aaaatcggcg ctcgacagtc agcagggtga
    18001 accgtggcaa acgattcgtt taatttctga attttacccg gaagacagtg gcctgttctc
    18061 cccgctgtta ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga
    18121 aacaccgcac gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt
    18181 gctgcgtgcg ggtctgacgc ctaagtacat tgatattccg gaactggttg ccaatgtgaa
    18241 attcgaagcc aaaccggcta accagttgtt gacccagccg gtgaaacaag gtgcagaact
    18301 ggacttcccg attccagtgg atgattttgc cttctcgctg catgacctta gtgataaaga
    18361 aaccaccatt agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt
    18421 gtggaaaggt tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa
    18481 cgaatcaccg gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca acaagctgta
    18541 agagcttact gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc
    18601 aggcggccag tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag
    18661 cggtaggcgt cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca
    18721 agaagattga aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga
    18781 cagctcctga atccaacctg gaagtgagtt atcaaaacta tcatcgcggc gtattcagca
    18841 gccagttgca actgttggtg aaacccattg ccgggaaaga aaatccgtgg attaaaagcg
    18901 gtcagagcgt catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta
    18961 aaaaactgaa cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag
    19021 taagcaaacc actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca
    19081 ttggttacag cggtgattcc agttccgata tttcgctcaa gccactgaat tacgagcaaa
    19141 aggatgaaaa agtcgccttt agcggcggcg agttccagtt aaatgctgac agagacggca
    19201 aagccatctc cctttccggg gaggcgcaaa gtggtcggat agacgcagtt aacgaataca
    19261 accagaaagt gcagttgacc tttaataatc tgaaaaccga cggttccagc acgctggcaa
    19321 gttttggtga gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg
    19381 aaggcaaaga actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca
    19441 atgacggtaa aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga
    19501 atcaggatct gggcagcggc aagctgactt taaaagtcgg ccagattgat ggtgaagcct
    19561 ggcatcagtt tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa
    19621 ttgccaacaa tcccgaactt tatcaggaga aagtgacgga agccttcttt agcgccctgc
    19681 cgctgatgtt gaaaggcgat ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc
    19741 agggtgaaag tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag
    19801 cgccgcaaac gctggcgcag gaagtagatc gttcggttaa atctctggat gcgaaactga
    19861 ccattccggt ggatatggca actgagttta tgactcaggt agcgaagctg gaaggttatc
    19921 aggaagatca agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc
    19981 agatgttccg tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatacta
    20041 acggtcagat aacgttaaac gggcagaaaa tgccactgga agatttcgtt ggtatgtttg
    20101 cgatgccagc tcttaacgtt ccggctgtac cggcaattcc gcagcagtaa ttcacggcca
    20161 aaacgccgga gaattttcac ttcggcgttt tcatctgaaa acaaaccaga gattgcctga
    20221 tgcgctacgc ttatcaggcc taccttttcc tgcaacactt tgaatttatg agtttttgta
    20281 ggctggataa ggcgtttaca ccgcatccgg cataaaaaac gcgcactttg tcaacaatct
    20341 gaaacgccgg aggtttttct ctccggcgtt atttctgact tcagcataaa gtcatactga
    20401 atactcattt tcacatccca gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt
    20461 tcccgccctt tatcggcagc aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc
    20521 cgatagttca taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat
    20581 tcatagccgc gagtcacaat ctgtgcaaat gtcagtgtaa attgcggcgt taaacgatag
    20641 ttgacgccca attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag
    20701 ggatagccaa tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca
    20761 ctgccaaata cgcttcccca cggcgcattc caggtcaatt gtccactaac caacgccgtt
    20821 tcattgggct ggctggtatt acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac
    20881 tcccctttca acgtataatt cgggtcgagc ggtatgcttt tctcgagaat actttgtatc
    20941 tgattggaaa catctttctg taccgatgcc cccagagaat agcgactggt atcatcatgc
    21001 caactggcgg ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta
    21061 tacacccctt catcacgaat accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc
    21121 cacattcccc acagatcaat atttggtgtt aaagcccaat aacctaatgc tccttcagaa
    21181 actgtcggta atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta
    21241 ccataactat aatccgtccc gaagcgacct gatttaagca ggctattttc actgggtttt
    21301 atttttactg cccccagggc aaagccaaaa cttttatcac catcaagata ccaccgggta
    21361 ctcatatcag cacgagcacc taatttataa acataatatg ccccgccttc aacgccaata
    21421 atattggaat aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata
    21481 tctcttcccg cgccagcact gggcttatcc gccctgcgca attcgttttt cagtctgatg
    21541 cgtagtcccg cctcatcgtc agccagttgc gccgcataag cagagataag gccagaggca
    21601 gccgtcaggc aaataacggc catggccact atttttctca tagttgcacc tttgatgttc
    21661 tgattatttt tattgattat taattagtga tatcgctgat taattgctgc tgcacttttt
    21721 tacgattatc aatttcaacc acgatttctt tgaatttttt atccgtaagc ggataaaacc
    21781 agataataac gaatgccagt agcataaatc cgcaaggtac taaggcaatt gatgtgcgga
    21841 tgcccataat aacttccggc gtttgcacct gattggcaat atatccgctt aaccccaaaa
    21901 taaaggcagg aattgaacct ccgattgcct gaccgcattt acgggtaaat gagaatagtg
    21961 aataggtgag cccttcaatt cgcacgccgg tcaggtattc accgtattct acggtatcag
    22021 cttccagtgc ccacatcact gtcatagtaa cgccctgacc aattgaagcg atggccaacg
    22081 caaccaacgc caccggcagt gaccagacgg aaacccagaa gaatagcaga taaccgcagg
    22141 ttcccagcaa agcgccaatc aggaaggtat tctttttacc gatcctcgcg accatccccg
    22201 gcacaagcgg tgccgatgcc acagtaccaa ccaggttttg caccagtacc agcacagtga
    22261 acagcccggt atcatttaac acatagcgca cgtagaacaa cgacgaggcg ctgaccgcaa
    22321 aggtcgaaat cagcacacac agcgcaccga tgcacaacat aaacagcggg cgattccgtt
    22381 tcagggtttg caaactgatc ttcaatgacg gctgcgcaac gatacgtacc acattctcac
    22441 gcgtcgattt gaagcagatg aagtaaagca ccattccggc aatcgccagc acaatcgtcc
    22501 agaaatggta taccgacacc atctcttccg ggctggagtt cttaatgctc ggccctatca
    22561 gaaatgccag gcagacaaag gtcaatgaag cggcaatccc acgagccgcg cccagacggg
    22621 cgcgggattg tggttgttgg gtcatcgcgg tagcaagtga accataagga atattcacca
    22681 ggctgtagca aaacccgagg cccatgtagg tcaaatatgc atacactact ttgcggctat
    22741 ggctccagtc ggtcggtacc cagaatacca gcacgctgaa gatcattaac ggcgcagtac
    22801 cgaagagtaa aaacgggcgg aattttcccc agcgggtatt cacactgtcc accactcgtc
    22861 cggcaaagac gtcggcgaag gcatcgaata cccgcaccag taacagcatg gtgcccgccg
    22921 cagcggcacc gacgccagcg acgtcggtgt agtaactcaa caggaagagc acccccattg
    22981 cgaaggcgaa gttattggcg acgtcaccga ggctgtagcc gacgatggtg cgccaggaga
    23041 gttgttgatt cattgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca
    23101 gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg tcgcgggtga agatcccctt
    23161 cttgttaccg ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac
    23221 ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc
    23281 acactgatac tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc
    23341 cacgccgtat tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc
    23401 tttttccagt accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata
    23461 acggttcagg cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa
    23521 cattacattg acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag
    23581 tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca
    23641 catcaccacg cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg
    23701 cgcttgctga gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc
    23761 cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac
    23821 cacgatgcca tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt
    23881 acggtaggag ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt
    23941 atcgaatcct ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa
    24001 cggtttgtgg ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg
    24061 aagcgggtag atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata
    24121 accttcaccc ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg
    24181 tccagttgca accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc
    24241 caccacctgc cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt
    24301 gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggatcccggc
    24361 atagttaaag aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac
    24421 cattcccggc gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac
    24481 acttttcccg gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg
    24541 atgctccatc acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa
    24601 acgcagcacg atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca
    24661 gacgttgccc gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg
    24721 cacagcaatt gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca
    24781 gttttcgcga tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg
    24841 ggtttctaca ggacgtaaca taagggactc ctcattaaga taaaaatact ggtcaacctt
    24901 taatctgatt agttaaccaa ttctgtgttg cacacagtta tctgtgagaa acccatggaa
    24961 gagaaatgga aataaaaaga actgacaccg cgatcaaaaa accaaaaact gcaataccca
    25021 cagcgtgata ttgatcgcat taatctttaa aacggcgttg acagaagcca taaaataagc
    25081 aaccgaacgt tcgttcgagc atattgggaa cgataaactc tcatgacata tggctacggt
    25141 gaatattttt gggggggaat tctccgatcc cggctagatt gtattttgta cagggaaatt
    25201 aaagtatgaa acgcgggcac agttatcacc agcccgcgaa atgagaggat caggatgcgg
    25261 ttaagatacc gccaatcatc gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat
    25321 cttccgcttc gatatcggcc agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca
    25381 ttgacgtgtt gatgtctggg cttatctcgc ctttttgctg ggcatcacgc atccgctggg
    25441 caacaaattc cgtgatcgtc atatgcttat ttttaagcat ggcggcgacc tgtgggttac
    25501 gcatcccttc cgccataatt tcgaccacca gcgcccgttg cccaaaggct tcatgggtga
    25561 gagagacaat agactcgacc atatagtcaa caaaatgaat ccctttaatc ggttcccgga
    25621 aacgagccag cgccctctcc tggtcctgta agataatcgc ctgaatcaag gcttctttgg
    25681 agatgaaatg gtgatagagc gtcccgggac taatggcgca agatttacag atggctttca
    25741 tcgaggcact gtgaaatcca ttttctgaaa aaatctctct ggcagcattg aggatccggg
    25801 tccgtgtcgg ttgtgcttca gtctgcatgt tatccatcat taaaccatta tagttagcgt
    25861 gaataactag tttaccgcgt tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa
    25921 ggcacactct attcgtatac ttttcactct gaaattggtt aaccaatagc cgtgatttgg
    25981 ctgccagtta tttagcagag acgttgatga gtttatcccg gtaaacaaca cgcacggttg
    26041 tttaccggtc cgttagtgta ttaattgagc tcctgtaccc caccaccgga gacggtgaga
    26101 atttgtccgc ttacccagct cgcagcaggc gagcaaagga acagcgctgc gttagcaata
    26161 tcttgcggtt ggcccagacg tctgatcggc gtgtgctgta acattttttg ttcaatttct
    26221 ggtgtaataa cggatttcag ggcatcggtt aatattgccc ccggcgcaat gccatttacc
    26281 cgaatatttt tttcacccag gtcaaacgcc atatttctga ccagatgact ggctgcagct
    26341 ttagatgatg cataggaagt catgtttata tttttatttt ctgccgccat agaagtgatg
    26401 gtcagaataa cgccaccgcc atttttttcc atttctggcg caacaagttg tgacagatgg
    26461 aaaaaagaaa acacattcag ttcataagca cggcgaaaat ccgccattgg catatcaaac
    26521 ggtttaggtc caccgccacc ggcgttgtta accagaatat caactttacc cagcttactg
    26581 atagcaaagt ctgccagtgc agagagttcc tgttcggaag taatatcaca acggcaggca
    26641 aatgcctgac cacccagttg ttgaatttcg tctacaacat ggttagctgc gtcggcgtta
    26701 atatcactga ccaccacaga tgcgccagct gtcgcgaatg taatggcgat ttctttacca
    26761 atacctgcac ccgcacctgt gatgatggcg catggcgcat ggcgcatggc gcatggcgca
    26821 tggcgcatgg cgcattttcc gtc
//
LOCUS       DAZPJR010000045        26756 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPJR010000045 DAZPJR010000000
VERSION     DAZPJR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.046"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            32..793
                     /gene="adiY"
                     /locus_tag="ACHVB7_001732"
     CDS             32..793
                     /gene="adiY"
                     /locus_tag="ACHVB7_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR1913742.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            930..2267
                     /gene="adiC"
                     /locus_tag="ACHVB7_001733"
     CDS             930..2267
                     /gene="adiC"
                     /locus_tag="ACHVB7_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR1913743.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2371..4014
                     /gene="eptA"
                     /locus_tag="ACHVB7_001734"
     CDS             2371..4014
                     /gene="eptA"
                     /locus_tag="ACHVB7_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR1913744.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            4011..4679
                     /gene="basR"
                     /locus_tag="ACHVB7_001735"
     CDS             4011..4679
                     /gene="basR"
                     /locus_tag="ACHVB7_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR1913745.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4680..5780
                     /gene="pmrB"
                     /locus_tag="ACHVB7_001736"
     CDS             4680..5780
                     /gene="pmrB"
                     /locus_tag="ACHVB7_001736"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR1913746.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5756..5845)
                     /gene="pmrR"
                     /locus_tag="ACHVB7_001737"
     CDS             complement(5756..5845)
                     /gene="pmrR"
                     /locus_tag="ACHVB7_001737"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR1913747.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5957..7459)
                     /gene="proP"
                     /locus_tag="ACHVB7_001738"
     CDS             complement(5957..7459)
                     /gene="proP"
                     /locus_tag="ACHVB7_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR1913748.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7723..8589)
                     /locus_tag="ACHVB7_001739"
     CDS             complement(7723..8589)
                     /locus_tag="ACHVB7_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR1913749.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(8586..10814)
                     /gene="crfC"
                     /locus_tag="ACHVB7_001740"
     CDS             complement(8586..10814)
                     /gene="crfC"
                     /locus_tag="ACHVB7_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR1913750.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            11227..11562
                     /locus_tag="ACHVB7_001741"
     CDS             11227..11562
                     /locus_tag="ACHVB7_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR1913751.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            11720..12163
                     /gene="yjdN"
                     /locus_tag="ACHVB7_001742"
                     /pseudo
     CDS             11720..12163
                     /gene="yjdN"
                     /locus_tag="ACHVB7_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            12296..13084
                     /gene="phnC"
                     /locus_tag="ACHVB7_001743"
     CDS             12296..13084
                     /gene="phnC"
                     /locus_tag="ACHVB7_001743"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR1913752.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            13109..14125
                     /gene="phnD"
                     /locus_tag="ACHVB7_001744"
     CDS             13109..14125
                     /gene="phnD"
                     /locus_tag="ACHVB7_001744"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1913753.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            14251..15030
                     /gene="phnE"
                     /locus_tag="ACHVB7_001745"
     CDS             14251..15030
                     /gene="phnE"
                     /locus_tag="ACHVB7_001745"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR1913754.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            15051..15776
                     /gene="phnF"
                     /locus_tag="ACHVB7_001746"
     CDS             15051..15776
                     /gene="phnF"
                     /locus_tag="ACHVB7_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR1913755.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            15777..16229
                     /gene="phnG"
                     /locus_tag="ACHVB7_001747"
     CDS             15777..16229
                     /gene="phnG"
                     /locus_tag="ACHVB7_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR1913756.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            16226..16810
                     /gene="phnH"
                     /locus_tag="ACHVB7_001748"
     CDS             16226..16810
                     /gene="phnH"
                     /locus_tag="ACHVB7_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR1913757.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            16810..17874
                     /gene="phnI"
                     /locus_tag="ACHVB7_001749"
     CDS             16810..17874
                     /gene="phnI"
                     /locus_tag="ACHVB7_001749"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR1913758.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            17867..18712
                     /gene="phnJ"
                     /locus_tag="ACHVB7_001750"
     CDS             17867..18712
                     /gene="phnJ"
                     /locus_tag="ACHVB7_001750"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR1913759.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            18709..19467
                     /gene="phnK"
                     /locus_tag="ACHVB7_001751"
     CDS             18709..19467
                     /gene="phnK"
                     /locus_tag="ACHVB7_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR1913760.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            19578..20258
                     /gene="phnL"
                     /locus_tag="ACHVB7_001752"
     CDS             19578..20258
                     /gene="phnL"
                     /locus_tag="ACHVB7_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR1913761.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            20255..21391
                     /gene="phnM"
                     /locus_tag="ACHVB7_001753"
     CDS             20255..21391
                     /gene="phnM"
                     /locus_tag="ACHVB7_001753"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR1913762.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            21391..21948
                     /gene="phnN"
                     /locus_tag="ACHVB7_001754"
     CDS             21391..21948
                     /gene="phnN"
                     /locus_tag="ACHVB7_001754"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR1913763.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            21935..22369
                     /gene="phnO"
                     /locus_tag="ACHVB7_001755"
     CDS             21935..22369
                     /gene="phnO"
                     /locus_tag="ACHVB7_001755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR1913764.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            22371..23129
                     /gene="phnP"
                     /locus_tag="ACHVB7_001756"
     CDS             22371..23129
                     /gene="phnP"
                     /locus_tag="ACHVB7_001756"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR1913765.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(23276..23605)
                     /gene="yjdP"
                     /locus_tag="ACHVB7_001757"
     CDS             complement(23276..23605)
                     /gene="yjdP"
                     /locus_tag="ACHVB7_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR1913766.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23674..23991)
                     /locus_tag="ACHVB7_001758"
     CDS             complement(23674..23991)
                     /locus_tag="ACHVB7_001758"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR1913767.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            24244..26229
                     /gene="yjcS"
                     /locus_tag="ACHVB7_001759"
     CDS             24244..26229
                     /gene="yjcS"
                     /locus_tag="ACHVB7_001759"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR1913768.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            26438..26713
                     /locus_tag="ACHVB7_001760"
     CDS             26438..26713
                     /locus_tag="ACHVB7_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR1913769.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         6356 a         7467 c         6991 g         5942 t
ORIGIN      
        1 tggtctttta ttcgcactca ggagtacatg tatgaggatt tgcagcgacc aaccttgtat
       61 tgttttattg actgaaaaag atgtctggat aagggtgaat gggaaagaac ctattagcct
      121 taaagctaac catatggcgt tattaaattg tgaaaataat attatcgacg tctcctctct
      181 taacaacact ttggttgctc atattagtca cgacatcatc aaagattacc tccggtttct
      241 gaataaagat ctctcgcaaa taccagtatg gcaacgtagc gctacgccca tactcaccct
      301 gccatgcctg acgccagacg tctttcgcgt tgccgcgcaa cacagcatga tgcccgcaga
      361 aactgagtca gaaaaggaac gaacacgcgc attattattc acggtgctat cccgttttct
      421 cgacagtaaa aaattccttt cattaatgat gtatatgtta cgtaattgtg taagtgacag
      481 cgtttatcaa attattgaaa gcgatattca caaagactgg aatcttagta tggtagccag
      541 ttgtttatgt cttagcccaa gtctgttaaa gaaaaagctg aaaagcgaaa acaccagtta
      601 tagccaaata atcaccacct gccgcatgcg ttatgccgta aatgaattaa tgatggacgg
      661 taaaaatatc tctcaggtat cacagtcctg cggctacaac agtacgtcgt actttatttc
      721 tgtctttaaa gacttctacg gtatgacgcc gctgcattat gttagtcagc acagagaacg
      781 aactgtcgcc tgatttttaa ccttaacgaa gagctatatt aataacggca tcagcgataa
      841 cccggtcgat aataattcaa ctatcgaatg caggcgtatg atatgacgta atttattgtc
      901 acgaagctcg ccttcgcagg agtttaatta tgtcttcgga tgctgatgct cacaaagtgg
      961 gcttaatccc cgtcaccctg atggtgtcgg ggaatattat ggggtcaggt gtttttctgt
     1021 tacctgcaaa cctggcctcc actggcggga ttgccattta tggatggttg gtgacgatta
     1081 tcggtgcgct ggggctctcg atggtatacg ccaaaatgtc gttcctcgat ccaagtcctg
     1141 gtggttctta cgcttacgcc cgccgctgct ttggcccgtt tctcggttat caaaccaacg
     1201 tcctctactg gctggcctgc tggatcggca atatcgctat ggtggtcatt ggcgtaggat
     1261 atttaagtta cttcttcccg attctgaaag atccattggt attaaccatc acctgcgtcg
     1321 tggtgctgtg gatcttcgtc ctgctgaaca ttgtcggtcc gaaaatgatc acccgtgtgc
     1381 aggccgttgc caccgtgctg gcgctgatcc ccatcgtcgg gattgccgta tttggctggt
     1441 tctggttccg tggtgaaacc tatatggcgg catggaacgt cagcggcctg ggcaccttcg
     1501 gtgcgattca aagtaccctt aacgttacgc tgtggtcgtt catcggtgtg gaaagtgcct
     1561 ccgttgccgc aggtgtggtg aaaaacccga aacgcaatgt ccctatcgcc accattggtg
     1621 gggtattgat tgccgccgtt tgctatgtgc tctccaccac cgcgattatg gggatgatcc
     1681 ctaatgccgc actgcgcgtt tctgcctcgc cattcggtga tgccgcacgg atggcgttgg
     1741 gtgacaccgc tggggccatt gtttccttct gcgcagctgc gggttgctta ggttcactgg
     1801 gcggctggac gttgctggcg ggtcaaacgg cgaaagccgc tgccgatgac ggactgttcc
     1861 caccgatttt tgcccgtgta aataaagcgg gtacgccagt ggcggggctg attatcgtcg
     1921 gtattttgat gaccatcttc cagctcagta gcatttcgcc aaacgcaacc aaagagttcg
     1981 gtctggtatc ttccgtgtcg gtcatcttta cactggtgcc atatctttac acctgtgcgg
     2041 cgttactgct gctcggccac ggtcactttg gtaaagcacg cccggcatat ctggctgtta
     2101 ctaccattgc cttcctctac tgcatctggg ccgtggtggg gtccggggcg aaagaggtta
     2161 tgtggtcatt tgtcaccctg atggtcatca ccgccatgta tgcgctgaat tacaaccggc
     2221 tacataaaaa cccgtatccc ttagatgcac caataagcaa agattaattc cccttaatcc
     2281 agcaaacata aaagccaacc ttaagaactt aaggttggct taattttgct ttgcgagcat
     2341 atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc gcctactaaa aagcccctct
     2401 ttgaatttac tcgcctggct attgttggcc gctttttata tctctatctg cctgaatatt
     2461 gcctttttta aacaggtgtt gcaggcgctg ccgctggatt cgctgcataa cgtactggtt
     2521 ttcttgtcga tgccggtcgt cgctttcagc gtgattaata ttgtcctgac actaagctct
     2581 ttcttatggc ttaatcgacc actggcctgc ctgtttattc tggttggcgc ggctgcacaa
     2641 tatttcataa tgacttacgg catcgtcatc gaccgctcga tgattgccaa tattattgat
     2701 accactccag cagaaagtta tgcgctgatg acaccgcaaa tgttattaac gctgggattc
     2761 agcggcgtgc ttgctgcgct gattgcctgc tggctaaaaa tcaaacctgc cacctcgcgt
     2821 ctgcgcagtg ttcttttccg tggagccaat attctggttt ctgtactgct gattttgctg
     2881 gtcgccgcac tgttttataa agactacgcc tcgttgttcc gcaataacaa agagctggtg
     2941 aaatccttaa gcccctctaa cagcattgtt gccagctggt catggtactc ccatcagcga
     3001 ctggcaaatc taccgctggt gcgaattggt gaagacgccc accgcaaccc gttaatgcag
     3061 aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg aaacctcgcg ggcggagaac
     3121 ttctccctca acggctaccc gcgtgaaact aacccgcggc tggcgaaaga taacgtggtc
     3181 tatttcccta ataccgcatc ttgcggcacg gcaacggcag tttcagtacc gtgcatgttc
     3241 tcggatatgc cgcgtgagca ttacaaagaa gagctggcac agcaccagga aggcgtgctg
     3301 gatatcattc agcaagcggg catcaacgtg ctgtggaatg acaacgatgg cggctgtaaa
     3361 ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc tgaatctgcc tggtcagtgc
     3421 atcaacggcg aatgctatga cgaagtgctg ttccacgggc tggaagagta catcaataac
     3481 ctgcagggtg atggcgtgat tgtcttacac accatcggca gccacggtcc gacctattac
     3541 aaccgctatc cgccgcagtt caggaaattt accccaacct gcgacaccaa tgagatccag
     3601 acctgtacca aagagcaact ggtgaacact tacgacaaca cgctggttta cgtcgactat
     3661 attgttgata aagcgattaa tctgctgaaa gaacatcagg ataaatttac caccagcctg
     3721 gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg
     3781 ccttatgcca tcgccccgga tagccaaaaa caggtgccga tgctgctgtg gctgtcggag
     3841 gattatcaaa aacggtatca ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa
     3901 cactattcac aagacaattt attctccacg ctattgggat taactggcgt tgagacgaag
     3961 tattaccagg ctgcggatga tgttctgcaa acttgcagga gagtgagtga atgaaaattc
     4021 tgattgttga agacgatacg ctgttattgc agggactgat tctggcggcg caaaccgaag
     4081 gctacacgtg cgatggcgta acaaccgcgc ggatggcgga acaaagcctt gaggccggtc
     4141 attacagcct ggtggtactg gatttagggt tacccgacga agatggactg cattttctcg
     4201 cccgtatccg gcagaaaaaa tacaccctgc cggtactgat cctcaccgct cgcgatacgc
     4261 tgaccgacaa aatcgccggg ctggatgtcg gtgccgatga ctatctggtg aagccttttg
     4321 cgctggaaga gttacacgcc cgtatccgcg ccctgctacg acgccataat aatcagggcg
     4381 aaagtgagct gattgttggc aatctgacgc tgaatatggg tcgccgtcag gtatggatgg
     4441 gcggtgaaga gttgattctg acgcccaaag aatatgctct gctgtcacgg ttaatgctca
     4501 aagcaggcag tccggtacat cgggaaattc tctacaacga catctataac tgggacaatg
     4561 aaccctcgac caacaccctg gaagtgcata tccacaatct gcgcgacaaa gtgggcaaag
     4621 cccgtatccg caccgtgcgc ggctttggct atatgctggt cgcgaatgag gaaaactaat
     4681 tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg ccaacggctg atattgacca
     4741 tcggggccat tttgttggtg tttgagctga tcagcatctt ctggctatgg catgaaagta
     4801 ccgagcagat tcagctgttt gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca
     4861 tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt ccccggcgtc tttatggtca
     4921 gcctgacgct atttatctgc tatcaggcgg tacgccgcat cacccgcccg ctggcggagc
     4981 tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac gcccattgcc attcacagcg
     5041 ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga tctggtcagt cgcctgacga
     5101 gcacgctgga taacgaaagg ttatttaccg ctgacgtcgc gcacgaactg cgaacgccac
     5161 tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac gcatcacatt gatgtagcac
     5221 cgttagtggc acggcttgat cagatgatgg agagcgtctc ccagctgctg caactggcgc
     5281 gtgccggaca gtcattttct tccggtaatt atcaacatgt aaaactgctg gaagatgtca
     5341 ttctcccctc gtatgacgaa ctcagtacca tgctcgacca gcgacagcaa accctgctat
     5401 tgccagagag cgccgccgac atcaccgttc agggtgatgc aaccctgctg cggatgttat
     5461 tgcgaaacct ggtagaaaac gcccatcgtt acagcccaca aggcagcaac attatgatta
     5521 agctgcaaga agatggcgga gcggtcatgg ccgttgaaga tgaaggaccg ggtattgatg
     5581 agagtaaatg cggggagttg agtaaagcgt ttgtacgtat ggacagccgt tatggcggga
     5641 ttggtctggg gttaagtatt gtcagccgca ttacacagtt gcatcacggg cagtttttcc
     5701 tgcaaaaccg gcaagagact tccggcacgc gggcctgggt acggctgaag aaagatcagt
     5761 acgtagcaaa ccagatataa agaaagctgc tgaccaccag cacactgaac acggtagtta
     5821 aactttcata aacacggttt ttcattgcac tctcctccgt taacctggag gagagtatgc
     5881 gcgtcagaga ttaagtgaac cttaagagtt caattggcct gataagacag cgtcacatca
     5941 ggccatccgt ttcagcttat tcatcaattc gcggatgttg ctgcaccagg cgggtacgtt
     6001 tcgcctgcaa atcggcaatc tcgtggtcaa tatcatcgat tttctgctcg atattatcgt
     6061 aatgctcgac gagaatttcc ttcgcttcct gtatatctga cgccgccggt gtcgcacctt
     6121 tcaacggacg atttgccgtc tctttcatgg ttacgccggt gattaaacca atcaccgcca
     6181 ctaccatcag gtaataggca ggcatcatca gattctgcga gctttcgacc agccaggccg
     6241 ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt aaatgccgcc gccagcgcgc
     6301 tgtaacggat atgcgtcggg aacatcgctg gcaaggtaga agccataacg cccgtaaagc
     6361 agttaaggat caccgccagc atcagtaacc cggcaaaaat caggccgatg acgttactgt
     6421 taatcagaat aaacgccggg atcgccaaca caaacagggc aacactacca agtagcacaa
     6481 acggacgacg gccaaaacgg tcactcagca agcccatcac cggctggaca aacagcatac
     6541 cgatcataat ggcgataata atcagcaccc cgtggtcttc ggagtaatgc aggttatgcg
     6601 acaaataact cggcatatag gtcagcaaca tgtagtaagt cacgttggtg gcaattacca
     6661 gaccaataca tgtcaacagg ctgcgccagt atttagtggc aatctcttta aacgagactt
     6721 tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag tttatcaaca tgctgctgga
     6781 acgccggagt ttcttccagc gcatggcgca ggtaaagccc gataatccct aacggcagag
     6841 caataaagaa cggaatacgc cagccccagt cgaggaagtt cgcttcgccg acaatggtcg
     6901 aaattaacac caccacgccc gcacccagca caaacccggc aatagaaccg aagtccagcc
     6961 agctgcccat aaagccacgt ttacggtcag gggagtattc cgcaacaaat atcgacgccc
     7021 cggtatattc accgccgacc gagaaacctt gtgccatctt acagatcaac agcagaatcg
     7081 gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc aatacagaac gtactgatcg
     7141 acataatcac aatagtgata gcgaggatct tctggcgacc atatttatcg cccaacatac
     7201 caaagaagag cccgccaagc ggtcgaatca gaaagggaac ggagaaagtg gcaagtgcag
     7261 caaccatctg cacgctgggg tcagcccccg ggaaaaaaac tttacctaat gcgtaagcaa
     7321 caaaaccata aacaccaaaa tcgaaccatt ccattgcatt acccagtgat gctgcggtaa
     7381 tggctttacg cagtttaccg tcatcaataa tggtgacatc acgaagggta atcggtttta
     7441 cttttttcct tttcagcata gctttcctcg cagagttggc ccatttccgc aaacgccaga
     7501 aaaatcagcg cgacggccgc aaaaggggag cgggaattgc aggatgcaat ctctgtaaca
     7561 agcgtagcgg gtttgcttac accctccggt taaggaatgt acaatcaaac cagttgacgc
     7621 ctgaattggg cagttaatga cctttttacc ctaccagtgt ttaaatttgt gatcaacttc
     7681 acacatggat ttcgtttagg actcattgat gtaactgatt atttacgcca aatttacaga
     7741 gaagatttcc tcgaccattg cccccaccaa acgctgcgct gaacagagtc gtggcatccc
     7801 cagcgcgact gtttgccagc gttgggtcac tgaccagcaa acgggctgtt tatctgccgt
     7861 ggaccacttt cccagccaac ttaaaaaatc agcatgctcc cctcccgaag aggcaaacca
     7921 ctgatggtaa tagtgacgcg tggtcggtgc cgcattgata ctctctgcca gatgttgtgt
     7981 cgctttctcg cgcaaatcac ccagtaacaa caccaccgct tgcgggcttc ccagacggca
     8041 ggcatcgcca aatgcacccc agcgcaactc ctccagaacg ataaaacagc gcccaggcaa
     8101 cgaccaggcg ttatacgccc cagcacgcca gcgggtaaaa atttgctcgc tctgatcacc
     8161 tgcctgcacc agtaggcctc gttgcgttaa cgccttttcc ttccagcgac aacgggctga
     8221 aaaccactca tacaaggcgg caatttgctg ggggagctcc gggaaagcag agtgctggcg
     8281 gtgatccatt ttttgcagca gcgccagatg ctgcgcgatc tgcgtcagcg ccagatgacc
     8341 gggatccagc atcgacgcca gtttttccac cattgtcagt cgcaggctaa gcggcgcatt
     8401 taacatgccg ccgttcgtcc atgtctgaag ctgcacacgc gaaagaagtt cctgcatcag
     8461 tcgttcacga aactgctgct gatgggcttg tggaaggcgg gcatcatcgc cctgcgccag
     8521 atccactaaa aagcggggat aaaccgactc cagcacgcga ccggggccgt ccagtaacgt
     8581 cttggtcaat atcgttctgc cgtgaaaagt gtttgaatat catcgcgtaa cagctgggcg
     8641 tcggtgtaaa tccagccgtg agtcatcaca gtctgctgca attgctgctg catcagcctg
     8701 accaccgatt cattttgttg acgcagagcc aggctttcgc gtaaacgcgt ctgtaattcc
     8761 gtcaaacatg aagcgaactc agcgcaaaaa gtattcatgc ctgccgtaac agattcatcg
     8821 acctgctctg ccagaacttt agccatttgt tggcaataaa gatcgacttc tgcgcttaaa
     8881 gctcgttgca acacactgta atcaaccgtc tctgtcgggg atttctcatt tccccgtccc
     8941 cagtcgggct gattcaacca gcgcgaaaaa gtctcacgca caacgcctaa acgcgtgctc
     9001 tgctcgtccg ttgcatcctg gcgcgaaatg acttcactga acagctggcg agtgttgacg
     9061 tggggaacta ctccgtgaaa aacaggaaaa tgaaccccag gacgaaaccc tgactcgctc
     9121 aattccattt tgacttgttg ctcaatgggc cgaataacat cggttaacac ccggcaaagg
     9181 gtggattcaa gctcggcaaa acgcagcgta aagtcgcgac tgatggtgtt ctgcgccgtc
     9241 tgtaacagtg tctcacagca ggtacgaatc tcgcttaacg gctccgaatc atcctgaaac
     9301 aaggcggcta actgcgcatt cagcgcatct tgctgttgac gcagaaagtg gttggcggag
     9361 gtcagggcca gctcgatttc atgtttaatc tcgccgctca cctgcgcctg attgagttgc
     9421 aatagctgca aactttcttc gacctgatgg atattttgcc gcaattgttc acaagcgacg
     9481 tttaacccgt gcgcacgaaa atccaggtat tcccgcgcct gctgcgcgta attcaacagt
     9541 ttatgcgcag cagatcgcag agcatacaac gaggcgttag cgtaagcggc atgaagcaac
     9601 gcctgaattg gctgggcgaa cagcgaatct tcccacaact gatcggcagc atgacgaata
     9661 tgttcgaggt ccgccagatc ggcatgacgc cagcgcctgc cgagcgcggc atgggcaaaa
     9721 tcttccaccc agcgttgttg ctctggcgct ggtaacttac cgttgttggc taactcatgg
     9781 cgcgctcgat ttgccaagta gccccacatc gacgacaccg gaaatatctg ctgtggcgta
     9841 atacagcctt tcatcagcgt cccggaaatc agtgcccgca cctggtcggc gtcgtcactg
     9901 ttacgatcct gttgatcgaa cttattgacc agcacataca gtggcaccga ttgccccacc
     9961 gccaaaatcg cctcacggac ctcttcatcg gagatcgatt tcagttgcgt ataatccagc
    10021 accgccagta ccgccgaggc gcgtgccagc tgctggttaa gcattttttg cagatgcggt
    10081 tgcccggctt catttggccc gggggtatcc agtaacgtca actgaccggg ataactctcc
    10141 agccccgcca gatggacaaa ctccacttca atcacgggaa tatgctcaat ggcggcgtaa
    10201 gcagaaaaag gaaaatcgac gtccagcgcc ttcgccagtc gcactaaatc attcaaactt
    10261 ttcagacaat gaaaaatagg ctgggcaccc agataatatt tttcgaaagc gacgccattt
    10321 tcgatccgct gcataagcgc acgcatatct ttatctattt ccagcacatc ggtcagatgc
    10381 ttaatatcgc aatcacgcag gcgctgttgt aattgttgaa ttaaacaatc gattggcgcg
    10441 acatgtgaaa aatgcagtac cggttccttt tgcccgggcg tatggcgaat aagcgtcggc
    10501 agcgcagtca ttgggcgatt acgattaggc agaacctccg taccaacaat ggcattaatg
    10561 gtggttgatt tccctgcttt catagtaccg acaatcgcca gcaccatttc cagtcgggaa
    10621 attttactca actcattatt cagcatcgcg tgacgttcgg cgatattagg ctgactccag
    10681 ggtaaagcca gttgtggcgc gtcgtctccg ggtacagaga gaggcatttt ttccagtaac
    10741 tgcaactgtt ggcgagaaag ctgtaacagg cgttcagcct cctgacttaa ctcatacagg
    10801 gtctgtgtgt acatagaaaa ttcttcctta aagcaaattt tgttactttt atttagccag
    10861 attgtttttg agttctgttt gagttctgtt tttggctttt ataattactg caagaaataa
    10921 ttttatattt agtgtgttgt tttttatcag aataaataac gtcttctgat gcgtttaaaa
    10981 cgtcagaaag ataaaaatat catgtgaata aaaaaaagaa caagtagagc attaacatta
    11041 tcttaaataa taaatagagg caaaaagatt attttctttt tgcgtttcct ttcaaatgaa
    11101 aacgatcgtc gtctaaaatc agcagtaccc ccgacaaact cagggatttt gtgtataatt
    11161 gcggcctttt tcggcaatct gccgtttttt ggcgcttttg ccctgctgac ttttgaggaa
    11221 atccacatgt cattaccaca ctgcccaaaa tgcaactccg aatacactta cgaagataac
    11281 ggcatgtaca tctgcccgga atgtgcctac gaatggaacg acgcagaacc tgcacaggaa
    11341 agcgacgagc tgatcgttaa agatgctaac ggcaatctgc tggctgacgg cgacagcgtt
    11401 accatcatta aagatctgaa ggtgaaaggt agctcttcga tgctgaaaat tggcaccaaa
    11461 gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg attgcaaaat cgacggtttt
    11521 ggtccgatga aactgaaatc tgagtttgtg aaaaagaact gattgtattg tgatcggtaa
    11581 gccggataag gcgctcgcgc cgcatccggc aacgatgccg actgcctgat gcgacgcttg
    11641 cgcgtcttat caggcagcct ctcatctgta taaatttcga actacactta attggcttct
    11701 cttaactgag gtcaccatca tgccgttaag tccctacctc tcttttgccg gtaactgtgc
    11761 cgacgcaatt gcttattatc aacgtacgtt gggcgctgaa ctgctctata aaatcagctt
    11821 cggcgaaatg ccaaaatcag cgcaggacag cgccgagaac tgcccttccg gaatgcaatt
    11881 tcccgatacc gccatcgccc acgccaatgt gcgcattgcc ggaagcgaca tcatgatgag
    11941 cgatgccatg ccgtcaggaa aagccagcta ctccggcttt acgctggtgc tcgattcgca
    12001 acaggtcgaa gaaggaaaac gctggtttga caatcttgcc gctaacggaa aaatcgaaat
    12061 ggcctggcag gaaactttct aggcgcatgg ctttggcaaa gtcaccgata aatttggcgt
    12121 accgtggatg attaatgtcg tcaaacaaca accaacgcaa taacccgccg ggaggcccgc
    12181 cctcccgcac tgtcatcgaa ttcccgttaa ctcttcatct gttagtcact tttaattaac
    12241 caaatcgtca caataatccg ccacgatgga gccacttttt tagggaggct gcatcatgca
    12301 aacgattatc cgtgtcgaga agctcgccaa aaccttcaat cagcatcagg cgctgcatgc
    12361 ggttgctctg aacattcatc acggtgaaat ggtggctctg cttgggccgt cgggttccgg
    12421 caaatccacc cttttacgtc acttaagcgg tttgattacc ggcgataaat ccgccggcag
    12481 ccatatcgag ctgctgggcc gcacagtcca gcgcgaaggc cgtctggcgc gcgatatccg
    12541 caaaagccgc gccaacaccg gctacatctt ccaacaattc aacctggtga accgcctgag
    12601 cgtactggag aacgtgctga ttggcgcgct cggcagcacg ccgttctggc gcacctgttt
    12661 tagctggttt acccgcgagc agaaacaacg cgcgttacag gcgctgaccc gcgttggcat
    12721 ggtgcatttt gcccatcaac gcgtttccac cctctccggc ggacagcagc agcgtgtggc
    12781 gattgcccgc gcgctgatgc agcaggcgaa ggtgattctg gccgatgaac ccatcgcctc
    12841 gctggacccg gaatccgccc gcatcgtgat ggacaccctg cgcgacatca atcagaacga
    12901 cggcatcacc gtggtcgtca cgctgcatca ggtggattac gccctgcgct actgcgaacg
    12961 catcgtcgcc ctgcgccagg ggcacgtttt ctacgacggc agcagccaac agtttgataa
    13021 cgaacgtttt gaccatctct accgcagcat taatcgcatc gaagagaacg cgaaagctgc
    13081 ctgacatccc catcattgag gaaaacgaat gaacgctaag ataattgcct cgctggcctt
    13141 caccagcatg ttcagcctca gcaccctgtt aagcccggca cacgccgaag agcaggaaaa
    13201 ggcgctgaat ttcggcatta tttcaacgga atcacagcaa aacctgaaac cgcaatggac
    13261 gccattctta caggatatgg agaagaagct gggcgtgaag gtgaacgcct tctttgcccc
    13321 agactacgca ggcattatcc agggaatgcg cttcaataaa gtggatatcg cctggtacgg
    13381 caacctgtcg gcaatggaag cggtggatcg cgccaacggc caggtcttcg cccagacggt
    13441 cgcggcggat ggatcgccag gttactggag cgtgttgatc gtcaacaaag atagtccgat
    13501 caacaacctg aacgatctgc tggcgaagcg gaaagatctc accttcggca atggcgatcc
    13561 taactccacc tctggcttcc tcgtccccgg ttactacgtc tttgccaaaa acaatatctc
    13621 cgccagcgac tttaagcgca ccgtcaacgc cgggcatgaa accaacgcgc tggccgtcgc
    13681 caacaagcag gtggatgttg ccaccaacaa caccgaaaac ctcgacaagc tgaaaacctc
    13741 cgcgccagag aagctgaaag aactgaaggt gatctggaag tcgccgctga tcccaggcga
    13801 tccgatcgtc tggcgcaaga atctctccga aaccaccaaa gacaagatct acgacttctt
    13861 tatgaactac ggaaaaacgc cggaagagaa agcggtgctg gaacgcctgg gctgggcacc
    13921 gtttcgcgcc tccagcgacc tgcaactggt gccgattcgc cagctcgcac tgtttaaaga
    13981 gattcagggc gtaaaaagca ataacggact gaatgagcag gacaagctgg cgaaaaccac
    14041 cgagattcag gcgcagctgg atgacctgga ccgcctgaac aacgcgttaa gcgcgatgag
    14101 ttcggtgagt aaagcggtgc agtaatgtgc tcgatcggtc ccctctccct tgagggagag
    14161 ggttagggtg agggggaaat gcgcacgctg atgccctcac cccggccctc tcccacaggg
    14221 agagggagaa aacattaaac ggagccaaac atgcaaacca tcaccatcgc cccacccaag
    14281 cgcagctggt tctcgcttct gagctgggcc attgttctcg ccgtgttggt cgtctcgtgg
    14341 cagggcgcgg aaatggcccc gcttacgctg atcaaagacg gcggcaacat ggcgaccttc
    14401 gccgccgact tcttcccgcc cgatttcagc cagtggcagg attacctcac cgaaatggcc
    14461 gtcacgctgc aaatcgccgt ctggggcacc gcgctggcag tggttctctc catccccttc
    14521 ggcctgatga gcgccgaaaa cctggtgccg tggtgggttt accagcccgt tcgccgcctg
    14581 atggacgcct gccgcgccat taacgaaatg gtcttcgcca tgctgttcgt ggtcgccgtc
    14641 ggtctcggac cgttcgctgg cgtgctggcg ctatttattc acaccaccgg cgtgctctcg
    14701 aaactgcttt ccgaagcggt ggaagcgatt gaacccggcc cggtggaagg cattcgcgcc
    14761 accggcgcca acaagctgga agagatcctt tacggcgtgc tgccacaggt aatgccgctg
    14821 ctgatctcct actccctcta tcgcttcgaa tccaacgtcc gctcggcgac cgtcgtcggc
    14881 atggtcggtg caggcgggat cggcgtcacc ctgtgggaag cgattcgcgg tttccagttc
    14941 caacaaacct gcgccctgat ggtgcttatc atcgtcacgg tcagcctgct ggatttcctc
    15001 tctcaacggt tgcgtaagca ctttatctga taagtgaggc attgatatct atgcacttgt
    15061 ctacacatcc gaccagctac ccaacacgct atcaggagat agccgcaaaa cttgagcagg
    15121 agcttcgtca acactatcgc tgcggcgact atcttcccgc cgagcagcaa ctggcggcgc
    15181 gctttgaggt aaaccgccac accctgcgcc gcgccatcga ccaactggtg gaaaaaggct
    15241 gggtacagcg ccgtcagggc atcggcgtgc tggtgctgat gcgcccgttc gattacccgc
    15301 tcaacgccca ggcgcgtttt agccagaacc tgctggacca gggcagccat cccaccagcg
    15361 aaaaactgct gtcggtattg cgccccgcgt ccggtcacgt tgctgacgcg ctggggatta
    15421 ccgagggtga gaacgttatc catttgcgca ccctgcgtcg cgtcaacggc gtcgcgctct
    15481 gtttaatcga ccactacttt gcggacctca ccctctggcc gacactgcaa cgtttcgaca
    15541 gcggttcgct gcacgatttt ctgcgcgagc aaaccggaat tgcgctgcgc cgcagccaga
    15601 cgcggatcag cgcccgccgc gcccaggcca aagagtgcca gcgccttgaa atcccgaata
    15661 tgtcgccgct gctgtgcgtg cgcaccctta accaccgtga cggtgaaagc agcccggcgg
    15721 agtactccgt cagcctgacg cgcgccgaca tgattgaatt cactatggag cactgaatgc
    15781 acgcagatac cgcgacccgc cagcactgga tgtccgtact ggcgcacagc caaccggctg
    15841 aactggcagc ccgcctgaac gcgctgaaca tcaccgccga ctatgaagtg atccgcactg
    15901 ccgaaactgg cctggtgcag attcaggcgc gaatgggcgg taccggcgaa cgcttttttg
    15961 ccggcgacgc cacgctgacc cgtgccgccg tacgcctgac tgacggcacc ctcggctaca
    16021 gctgggtgct ggggcgtgat aaacagcacg ccgaacgctg cgcgctgatt gatgcgctga
    16081 tgcagcaatc tcgccacttt caaaacttat cagaaaccct tattgccccg ctggacgctg
    16141 accgtatggc acgcattgcc gcacgccagg ccgaagtgaa cgccagccgg gtcgacttct
    16201 ttacgatggt tcgcggagac aacgcatgac cctggaaacc gcttttatgc ttcccgtgca
    16261 ggatgcccag cacagttttc gtcgcctgtt aaaggccatg agcgagccgg gcgtgattgt
    16321 cgccctgtat cagctcaaac gcggctggca gccgctgaat atcgccacca ccagcgtgct
    16381 gctgacgctg gccgataacg acacgccggt gtggcttgcc acgccattaa gtaacgatat
    16441 cgtcaatcag agcctgcgtt ttcataccaa cgcgccgctg gtcagccagc cggaacaggc
    16501 gaccttcgcg gtgacggatg aggcgatttc cagcgaacag ctcaacgccc tttccaccga
    16561 caccgccgtt gcgccggaag cgggcgcgac gctgatttta caggtcgcca gcctgagcgg
    16621 cggacgcatg ttgcgtctca ccggcgcggg cattgccgaa gaacgaatga tcgctccgca
    16681 gctgccagag tgcattctgc acgaactcac cgagcgcccg cacccgttcc cgctcggcat
    16741 cgacctaatc ctgacctgcg gcgagcgcct gctggctatc ccgcgaacca ctcatgtgga
    16801 ggtgtgctga tgtacgttgc cgtgaaaggg ggcgaaaagg cgatcgacgc cgcccacgcc
    16861 ctgcaagaga gccgacgccg gggcgatacc gatttgcccg aactgagcgt cgcccagatt
    16921 gaacagcagc ttaacctcgc ggtggatcgc gtgatgacag aaggcggcat tgccgaccgc
    16981 gaactggcgg cgctggcgct gaaacaggcc agcggcgata acgtcgaagc gattttcctg
    17041 ctacgcgcct accgcaccac gctgacgaaa ctggcggtaa gcgagccgct cgacaccacg
    17101 gagatgcgtc tcgaacggcg tatttcagcg gtttataaag atattcccgg cggccagctg
    17161 cttggcccaa cctacgacta cacccatcgc ctgctcgatt tcactctgct ggcaaacggc
    17221 gaagcgccga cgctgaccac cgccgacagc gaacaacagc cgtcgccgca cgttttcagc
    17281 ctgctggcgc gtcaggggct ggcgaagttt gaagaggata gcggagcaca gccggatgac
    17341 atcacccgca cgccgccggt ttacccctgt tcacgttctt cccgtttgca gcagttaatg
    17401 cgcggcgacg aaggctattt gctggcgctg gcttactcca cccagcgcgg ttacgggcgc
    17461 aaccacccgt tcgcgggcga gatccgcagt ggttacatcg acgtgtcgat tgtgccggaa
    17521 gagctgggat ttgcggtaaa cgtcggcgaa ctgctgatga ccgagtgtga aatggtcaac
    17581 ggttttatcg acccgccgga tgagccgccg cacttcacgc gcggctacgg gctggtattc
    17641 ggcatgagcg agcgcaaagc gatggcaatg gcgctggttg accgtgcgtt gcaagccccg
    17701 gaatacggcg agcacgcgac aggcccggcg caggatgaag agttcgtgct ggcgcatgcc
    17761 gacaacgtcg aagccgcagg ctttgtctcg cacctcaaac tcccgcacta cgtcgatttc
    17821 caggccgaac tggagctact caaacgtctg caacaggagc agaaccatgg ctaatctgag
    17881 cggctacaac tttgcctacc tcgacgagca gaccaaacgc atgatccgcc gcgccatctt
    17941 aaaagcggtg gcgatccccg gttatcaggt accgtttggc gggcgcgaga tgccgatgcc
    18001 gtacggctgg ggaactggtg gtatccagct caccgccagc gtgattggcg aaagcgacgt
    18061 gttgaaggtg attgaccagg gtgcggatga caccaccaac gccgtgtcga ttcgcaactt
    18121 ctttaagcgc gtgaccgggg taaacaccac tgaatgtacg gacgatgcga cgcttatcca
    18181 gacgcgtcac cgcatccccg aaacgccgct gaccgaagat cagatcatta tcttccaggt
    18241 gccaatcccg gaaccgctgc gctttatcga gccgcgcgaa acggagaccc gcactatgca
    18301 cgcgctggag gagtacggcg tgatgcaggt gaaactgtat gaagatatcg cccgcttcgg
    18361 tcatatcgcc accacctatg cttatccggt gaaggtgaac gggcgctacg tgatggaccc
    18421 gtcgccgatc ccgaaattcg ataacccgaa aatggacatg atgcccgccc tgcagctgtt
    18481 cggcgcaggg cgcgagaagc gcatctatgc ggtgccgccg tttacccgcg tggaaagtct
    18541 cgatttcgac gatcacccgt tcaccgttca gcagtgggat gagccatgcg ccatctgcgg
    18601 atcgacccac agctatcttg atgaagtggt gctggatgac gccggaaacc gcatgtttgt
    18661 ctgctccgat accgattatt gccgccaaca gagcgaggcg aaaagccaat gaatcaaccg
    18721 ttactttcgg tcaataacct gacccacctt tacgcgccgg gcaaaggctt tagcgatgtc
    18781 tcttttgatt tatggccggg ggaagtgctg ggcattgtcg gggaatccgg ctccgggaag
    18841 accacgctgc tgaagtcgat ctccgcgcgt ctgacgccgc agcaggggga gattcactat
    18901 gaaaaccgtt ccctctatgc aatgagcgag gccgaccgcc gtcgcctgct gcgcaccgaa
    18961 tggggcgtgg tgcatcagca tccgctcgac ggcctgcgcc gccaggtgtc ggcaggcggc
    19021 aatatcggcg agcggctgat ggcgaccggg gcgcgacatt acggcgatat tcgcgctacc
    19081 gcgcagaagt ggctggaaga ggtggagatt cccgccaacc gtatcgacga cctgccgacc
    19141 accttttccg gcggtatgca gcagcgtttg cagattgccc gcaacctggt gacgcatccg
    19201 aagctggtgt ttatggatga accgaccggc gggctggatg tgtcggtgca ggcccgcctg
    19261 ctcgacctgc tgcgcggact ggtggtggag ctgaacctcg cggtagtgat tgtcacccac
    19321 gatttaggcg tcgcccgcct gctggcggac cgtttgctgg tgatgaagca ggggcaagtg
    19381 gtggagagtg ggttaaccga ccgcgtgctc gacgacccgc atcatccgta tacacagctg
    19441 ctggtgtcat cggttttgca gaattgagcc ggtgccggat gcggcgtaaa cgccttatcc
    19501 ggcctacaaa tgcgctcccc gtaggtcgga taagacgcgt cagcgtcgca tccgacaccc
    19561 gaaccacgag gcgaaaaatg attaacgtac aaaacgtcag taaaaccttc atcctgcacc
    19621 agcaaaacgg cgtgcgcctg cccgtcctca accgcgcctc gctcatcgtc aacgcgggcg
    19681 aatgcgtggt gctccacggc cattccggca gcggaaaatc aaccctgcta cgctcgctgt
    19741 acgccaacta tctgcccgac gaagggcaaa tccagatcaa acacggtgac gaatgggtcg
    19801 atctggtcac cgcgccagcg cgcaaagtgg tggagatccg caaaaccacc gttggctggg
    19861 tgagccagtt tctgcgcgtc atcccgcgta tttcggcgct ggaagtggtg atgcagccgc
    19921 tactcgatac cggcgtaccg cgtgaagcct gcgccgctaa agccgcgcgt ctgctcaccc
    19981 gcctgaacgt gccggaacgc ctgtggcatc tggcgccatc gaccttctcc ggcggcgaac
    20041 agcagcgcgt caacatcgcc cgcggcttta tcgtcgacta ccccattctg ctgctcgacg
    20101 aacctaccgc ctcgctggac gccaaaaaca gcgccgccgt agtcgagctg attcacgaag
    20161 ccaaagcccg tggcgcggcc atcgtcggca tcttccacga cgaagccgta cgtaatgacg
    20221 ttgccgaccg cctgcaccca atggaagcct cttcatgatt atcaataacg tcaaattggt
    20281 gctggaaaac gaagtggtac acggttcact ggaggtgcag gacggcgaaa tccgcgcctt
    20341 tgccgaaagc cagagccgcc tgccggaggc gatggacggc gaaggcggct ggctgctgcc
    20401 ggggctgatt gagctgcata ccgataatct ggataaattc ttcaccccgc gcccgaaagt
    20461 tgactggcct gcccactcgg cgatgagcag ccacgacgcg ctgatggtgg cgagcggcat
    20521 caccaccgta ctggatgccg tggcaattgg cgacgtgcgc gacggcggcg atcggctggt
    20581 gaatctggag aagatgatca acgccatcga agagacgcag aaacgcggcg tcaaccgcgc
    20641 cgagcaccgt ctgcatctgc gctgcgaact gccgcatcac accacgctgc cgctgtttga
    20701 aaaactggtg cagcgcgagc cggtgacgct ggtgtcgctg atggaccact cgccgggcca
    20761 gcgccagttc gccaatcgcg agaagtatcg cgaatattat cagggcaaat actccctcac
    20821 tgatgcgcag atgcagcagt acgaagaaga gcaactggcg ctcgccgcac gctggtcgca
    20881 gccgaatcgc gaatccatcg ccgccctgtg ccgcgcgcga aaaattgcgc ttgccagcca
    20941 cgatgacgcc acccacgccc acgttgctga atctcaccag cttggcagcg tgatcgccga
    21001 atttcccacc acgttcgaag cggcggaagc ctcgcgcaag catggcatga acgtgctgat
    21061 gggcgcgccg aatattgtgc gcggcggctc gcactccggc aacgtggcgg ccagtgaact
    21121 ggcgcagctt ggcctgctgg atatcctctc ttccgactac taccccgcca gcctgctcga
    21181 tgcggcattt cgcgtcgccg atgacgagag caaccgcttt acgctgccgc aggcggtgaa
    21241 gctggtgact aaaaatccag cgcaggcgct taatctccag gatcgcgggg tgattggcga
    21301 gggcaaacgc gcggacttgg tgctggcgca tcgcaagggc aatcacattc atatcgacca
    21361 cgtctggcgt cagggtaaaa gggtgttctg atgatgggaa aactgatttg gttaatgggg
    21421 ccgtccggct ccgggaaaga tagcctgctg gcggaactcc gcctgcggga acaaactcag
    21481 ctactggtgg cgcatcgcta catcacgcgc gacgccagcg ccggaagcga aaaccatatc
    21541 gctctgagcg agcaggagtt ttttacccgc gcggggcaga acctactggc cttaagctgg
    21601 cacgctaacg gtctgtatta tggcgtcggc gtcgagatcg acctctggct gcacgccgga
    21661 ttcgacgtgc tggtcaacgg ctcacgcgcc catctgccgc aggcgcgggc gcgctatcaa
    21721 tcggcgctgc tgcccgtctg tttacaggtt tcgccggaga tcctccgcca gcgcctggaa
    21781 aaccgtggcc gtgaaaacgc cagtgaaatt aacgcccgcc tggcgcgcgc cgcccgctat
    21841 actccacagg attgccatac gctcaacaat gacggcagcc tgcgccagtc ggtcgacacg
    21901 ctgctgacgc tgattcatca gaaggagaaa caccatgcct gcttgtgagc ttcgcccggc
    21961 cacgcagtat gataccgacg cggtttacgc gctgatttgt gagctaaaac aggcggagtt
    22021 tgaccaccac gcatttcgcg tgggctttag cgccaatctg cgcgacccga acatgcgcta
    22081 tcatctggcg ctgctggatg gcgaggttgt cggcatgatc ggcctgcatt tgcagtttca
    22141 tctgcatcat gtcaactgga tcggcgaaat tcaggaactg gtagtgatgc cgcaggcgcg
    22201 cggcctgaac gtcggtagca agttgctggc gtgggcagaa gaagaagccc gccaggccgg
    22261 ggccgaaatg accgagcttt cgaccaacgt gaagcgccac gacgcgcacc gtttctatct
    22321 gcgagaaggc tacgagcaga gtcacttccg cttcaccaag gcgctgtaac atgagtctga
    22381 ccctcacgct caccggcacc ggcggcgcac agggcgttcc ggcatggggc tgcgagtgcg
    22441 cggcctgcga ccgagcgcgg cgctcgccgc agtatcgccg ccaaccgtgc agcggcgtag
    22501 tgaagtttaa cgaggcgatc accctgatcg acgccgggct gcacgatctc accgatcgct
    22561 ggtctcccgg atcgttccag cagtttttgc tgacgcatta tcatatggat cacgtccagg
    22621 ggctgtttcc gctgcgctgg ggcgtgggcg atccgatccc gatctacggc ccgccggatg
    22681 aacagggctg cgacgatctg tttaaacatc ccgggctgct tgatttcagc cacacggtgg
    22741 aaccgtttgt ggtgtttgat ttgcaggggt tgcaggtcac gccgctgccg cttaaccact
    22801 caaaactcac tttcggctat ttactggaaa cggcgcacag ccgggtggcg tggctgtctg
    22861 acaccgctgg cttgccggaa aaaacgctga aatttttacg caataatcag ccgcaggtaa
    22921 tggtgatgga ttgcagtcac ccgccgcgcg cggatgcacc gcgtaatcac tgtgatttaa
    22981 ataccgtgct tgcgctgaat caggttatcc gctcgccacg ggtgattctg acccatatca
    23041 gccaccagtt tgatgcgtgg ctgatggaaa acgcactacc gtcagggttt gaggtggggt
    23101 ttgatgggat ggagattggg gtggcgtgat gagagggaat gtgcgcgctg gccccctcac
    23161 cctaaccctc tccccagagg ggcgagggga ccgattgtgc tcaatattga atattgcgct
    23221 cgttttctcc ctctccccat tggggtgagg ggcgatgcct gctccatacc caacctcatc
    23281 gcccatactc atcttccatt ctccgctctt catcctccag ttgccgacgc tcctgatcaa
    23341 gctggcgctg gcgatcgtcc agctgcctgc ggcgatcttc aaactggcgg cggcggtcgt
    23401 catattgtct gcgccgatcg tcgctcactt cacgctgcca gccgtcgtcg cgcgaatctt
    23461 catagtctcg cccacggtca gggttataag cgtcattaat cgcctgctga atattaccaa
    23521 tagtatcgtc gataatatca gcctgggccg gaacggtgga cagcgtgagc agggtgaata
    23581 aaagaaatag cggaaagcgt ttcattagcc aacctcaaaa agaaactcta tccacattag
    23641 tcattactca tccatgcaag tagtggatga atctcaattt ctccgctgct ctattgccgt
    23701 aatcgcctcc acgcgttgtt gatgacgacc gccttcgtac tgtgcgccca gccacgcatc
    23761 cacaatcatt tttgccagtt cgaggccaac cactcgtgaa ccaaaagcca gtgcaacttc
    23821 actggcataa tgcggataat cagtgcgctc tgacgacctg gtgtctttat caatcacctc
    23881 actcattgac catgaataaa aggctggcac agcaacgcag aatcctgcgg tgccagcgac
    23941 atgaccgccc attgctccgg tacgtttttt aaaaagaaac accggttgca ttgataaatt
    24001 gtgctcagta tttgccgaag tacaatatat ataataaata tataaaacac ctcatgttat
    24061 ttacagagat aataaattcg cgtaattttc cctcttcagc acaaatgcct aataaaatgg
    24121 atcgttcagc tcacacttcc acttattaaa gtcgcaaatt cccaattatg ataaccccag
    24181 ctttaaatga cgtattttgc atggattaca tatttacgac ataaatgata aatggagatt
    24241 tacatgaata actctcggtt attccgtttg agcagaattg ttattgcgtt aactgccgcc
    24301 agcggcatga tggtaaatac cgctaacgcg aaagaggaag cgaaagccgc cactcaatat
    24361 acccaacagg ttaatcagaa ttacgccaaa tcattaccgt ttagcgatcg tcaggatttt
    24421 gacgacgctc agcgtggatt tatcgctccg ctgctggatg aaggtattct gcgcgatgct
    24481 aatggcaaac catattatcg cggggaagat tataaatttg atatcaatgc gcccgcgccc
    24541 gaaaccgtta accctagcct gtggcgtcag tcgcagttaa acggtatttc cggcctgttt
    24601 aaagtcaccg acagaatgta ccaggttcgc ggtcaggata tctccaacat cacctttatc
    24661 gaaggtgata ccgggattat tgtcatcgac ccgctggtga cgccaccgag cgcaaaagcc
    24721 gcccttgacc tttacttcca aaatcgcccg caaaaaccga ttgttgcggt tatttatacc
    24781 cacagccata ccgaccacta tggcggcgtg aaaggcatta tctctgaagc tgatgtgaaa
    24841 tccggtaaag tgcaggttat cgcccctgct ggctttatgg acgaagccat cagcgaaaac
    24901 gtactggcgg gtaatattat gagccgccgt gcactttact cctacggcct gctgctggcg
    24961 cataaccctc agggtaacat cggcaatggt cttggcgtaa cgctggcatc gggctacccg
    25021 agcatcatcg caccgaacaa aaccatcacc aaaaccggtg agaagatgat tatcgacggc
    25081 ctggagtttg acttcctgat gaccccaggt agcgaagcac cagccgaaat gcacttctat
    25141 attccggccc tgaaagcgct gtgtaccgcc gagaacgcca cgcataccct gcacaacttc
    25201 tacactctgc gcggcgcgaa aacccgcgac accagcaagt ggaccgagta tctgaacgaa
    25261 acgctggata tgtggggtaa cgacgcggaa gtcctgttta tgccgcacac ctggccggtc
    25321 tggggcaata agcatatcaa tgattatatt ggtaaatatc gcgatactat caagtacatt
    25381 cacgaccaga ccctgcacct ggcgaaccag ggctacacca tgaatgaaat cggcgacatg
    25441 attaaactgc cgcctgcact tgccaataac tgggccagcc gtggctatta cggttctgtc
    25501 agccacaacg cccgcgcggt gtataacttc tatcttggct attacgacgg taacccggct
    25561 aacctgcatc cgtatggtca ggtggagatg ggtaaacgtt acgtgcaggc gctgggcggt
    25621 tctgcccgtg tcatcaacct ggcgcaagaa gcgaacaagc aaggtgatta ccgctggtcg
    25681 gcagaactgc tgaaacaggt gattgccgcc aacccgggtg accaggtcgc gaagaatctg
    25741 caagcgaata actttgaaca gctgggctat caggccgagt ccgccacctg gcgcggtttc
    25801 tacctgaccg gcgcgaaaga gctgcgcgaa ggggtgcata agttcagcca cggcaccatc
    25861 ggttccccgg acaccattcg cgggatgtcg gtcgaaatgc tgttcgactt tatgtccgtt
    25921 cgcctcgata gcgcgaaagc cgcgggtaaa aatatcagcc tgaacttcaa tatgagcaat
    25981 ggcgataacc tcaacctgac gctgaacgat agcgtgctta actaccgtaa aacactgcaa
    26041 tcccaagctg acgcctcttt ctacatcagc cgtgaagatc tgcacgccgt gctgaccggg
    26101 caagccaaaa tggcggatct ggtaaaagcg aagaaagcca aaattattgg caatggcgcg
    26161 aaactggaag aaattatcgc ctgtctggat aatttcgatt tgtgggtgaa tatcgtaacc
    26221 ccaaattaat gtcactcgct tttgggggcg ctgaagcccc caaaagcatt tattttaaaa
    26281 atatatacag ccaattaata aataaaatat ataagttatg aatgcggtga ctgaataaag
    26341 ctatttttat tcatcgagaa aatagccata ctgacgcctt caatggatat tgatattcac
    26401 tacagatatt gttcgcagca gacaggatgt atttaatatg ccaacagttc tctctcgcat
    26461 ggcgatgcaa ctcaaaaaaa cagcctggat aattcccgtc ttcatggttt cgggatgctc
    26521 attatctccg gcaatcccgg tgatcggtgc ttattatccc agctggtttt tctgcgccat
    26581 tgccagcctt attttgatgc tcatcacgag gcgagttatt cagcgggcaa atatcaatct
    26641 ggcatttgtc ggaattattt ataccgccct ttttgctctc tacgccatgc tgttctggct
    26701 ggcatttttc taatctattt gagacgcgct catggaaagt acgccgaaaa aagctc
//
LOCUS       DAZPJR010000046        25647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPJR010000046 DAZPJR010000000
VERSION     DAZPJR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.047"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHVB7_001761"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHVB7_001761"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR1913770.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHVB7_001762"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHVB7_001762"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR1913771.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHVB7_001763"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHVB7_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR1913772.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHVB7_001764"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHVB7_001764"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR1913773.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="ACHVB7_001765"
     CDS             complement(5499..5696)
                     /locus_tag="ACHVB7_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR1913774.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHVB7_001766"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHVB7_001766"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR1913775.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHVB7_001767"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHVB7_001767"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR1913776.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHVB7_001768"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHVB7_001768"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR1913777.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHVB7_001769"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHVB7_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR1913778.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHVB7_001770"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHVB7_001770"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR1913779.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHVB7_001771"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHVB7_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR1913780.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHVB7_001772"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHVB7_001772"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR1913781.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHVB7_001773"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHVB7_001773"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR1913782.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHVB7_001774"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHVB7_001774"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR1913783.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="ACHVB7_001775"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="ACHVB7_001775"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR1913784.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="ACHVB7_001776"
     CDS             complement(17138..17191)
                     /locus_tag="ACHVB7_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913785.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="ACHVB7_001777"
     tRNA            complement(17425..17500)
                     /locus_tag="ACHVB7_001777"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
     gene            complement(17536..17611)
                     /locus_tag="ACHVB7_001778"
     tRNA            complement(17536..17611)
                     /locus_tag="ACHVB7_001778"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17576..17578),aa:Gly,seq:gcc)
     gene            complement(17769..17844)
                     /locus_tag="ACHVB7_001779"
     tRNA            complement(17769..17844)
                     /locus_tag="ACHVB7_001779"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17809..17811),aa:Gly,seq:gcc)
     gene            complement(18055..18600)
                     /gene="orn"
                     /locus_tag="ACHVB7_001780"
     CDS             complement(18055..18600)
                     /gene="orn"
                     /locus_tag="ACHVB7_001780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR1913786.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18695..19747
                     /gene="rsgA"
                     /locus_tag="ACHVB7_001781"
     CDS             18695..19747
                     /gene="rsgA"
                     /locus_tag="ACHVB7_001781"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR1913787.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19844..20812
                     /gene="asd"
                     /locus_tag="ACHVB7_001782"
     CDS             19844..20812
                     /gene="asd"
                     /locus_tag="ACHVB7_001782"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR1913788.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20834..24157
                     /gene="mscM"
                     /locus_tag="ACHVB7_001783"
     CDS             20834..24157
                     /gene="mscM"
                     /locus_tag="ACHVB7_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR1913789.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="ACHVB7_001784"
     CDS             complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="ACHVB7_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR1913790.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5934 a         7194 c         6499 g         6020 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac
    17521 agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17581 ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgaa ccgctactgc
    17641 cttggctgcc ttcactcacc ttagtcacat acttttgtaa gttcccaagg attcgttcag
    17701 ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt gcagcacttt
    17761 tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    17821 ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat tttcgcattt
    17881 ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac cccccaaaaa
    17941 agtttttttt gcgtttttat ctcaagtgct gaaattatca gcaagacgtt caatttagcg
    18001 tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa aattttacag
    18061 cttgataaaa tgctcgcggt agtaagccag ctccgccacc gattcacgga tatcatccat
    18121 tgcctgatgc gtcccctgct tggtaaaacc atccagaatt tccggcttcc agcggcgcgc
    18181 cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt ccagctccgg
    18241 catgtattta aacaggaaac gacggtcctg tccgatgctg ttaccgcaaa tcggcgattt
    18301 tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt cccgatcgcc
    18361 catcgtgctc gctttcacgc gctctaccag cccgctggcg gtatgggtgc gcacgttcca
    18421 gtcatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg gcccttctgc
    18481 cagaatattc agattggcat cggtcaccag cgtggcaatc tcaataatgc gatcgcgctc
    18541 gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat tggcactcat
    18601 gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta tcatagatgt
    18661 tttgcccatc aggggcgacc aggagtcagt acgattgagt aaaaataaac tctccaaagg
    18721 ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta aggagaagcc
    18781 cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca gccgctttgg
    18841 tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata ttcgccgtac
    18901 catccgttcg ctggtaaccg gcgaccgcgt agtctggcgt ccgggtaaac cggcggcgga
    18961 aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgtacctcgg tgttgacgcg
    19021 tccggatttc tacgacggcg taaaacctat tgccgccaac atcgaccaga ttgtcattgt
    19081 ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctag tggcctgcga
    19141 aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg acgacgaagg
    19201 catggcgttc gtcaacgagc agatggatat ctaccgcaat atcggttatc gcgtattgat
    19261 ggtttccagc catactcagg atgggctaaa accgctggaa gaggcgttga ccgggcgcat
    19321 cagcattttt gccgggcagt ctggcgtcgg caaatccagc ctgctgaacg cgttgctggg
    19381 gctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg gtcagcacac
    19441 cacgaccgcc gctcggctgt atcacttccc gcacggcggt gatgtgattg attcccctgg
    19501 tgtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg gctttgtcga
    19561 attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata ccgatccggg
    19621 ctgcgctatc cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt tcgaaaacta
    19681 tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt ctgatacgga
    19741 tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg atccggcatg
    19801 taggccgaat caggaacgac aaaacaatgg cctggaggct accttgttaa attcatttaa
    19861 actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg gttggggcgc
    19921 aagcaagcgg gcaggatggt tgacaaaact ggttatcgat ctgttcgtta aatactacaa
    19981 ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct ttaacgaatt
    20041 ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga atgtactggt
    20101 catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata aaatcctgca
    20161 agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc tgatggcgga
    20221 cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact accaccgcgt
    20281 acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg atctcttctc
    20341 cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg aacgcgtgat
    20401 ttgccttttc gataccgaat ttggcccaat ggcgcagatt ctggtcggag cgacgattgt
    20461 tggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag gtatcatcaa
    20521 gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga aaggccagga
    20581 aatgggtcgc tttaaactcg gttccaccgt tatcaacctg tttgcaccgg gtaaagtgaa
    20641 tctggttgag caactggaaa gcctgtctgt cacgaaaatt tgccagccgc tggcagtatc
    20701 taccgaaacc tttgttacgc cagacgctga acctgccccg cttcctgctg aagagatcga
    20761 agcagaacac gacgccagcc cattggttga cgacaaaaaa gaccaggtct aatccatcaa
    20821 aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc tcagttgggg
    20881 ggcgtacgcc gcgacggccc ccgatagcaa acaaatcact caggaactgg agcaggcaaa
    20941 agcggcgaaa cccgcacagc cggaagtcgt agaggcgctt cagtctgcct taaatgcgct
    21001 tgaggaacga aaaggttccc ttgagcgcat caaacaatat cagcaagtta tcgataatta
    21061 tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgaca agccgcgcag
    21121 cgtgtcgccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg tcagcagcca
    21181 gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg agattgccga
    21241 ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa atgagatcga
    21301 gcgccgcctg ggaacgctta ccggcaatac tccgctcaat caggcacaaa atttcgcgtt
    21361 gcagtctgac tctgcacgtc ttaaggcgct cgttgatgaa ctggagctgg cgcagctgtc
    21421 tgccaataac cgccaagaat tagcgcgctt acgctcagag cttgcggaaa aagagagcca
    21481 gcaactggat gcgtatttgc aggccttacg taatcagttg aatagccaac gtcagcttga
    21541 ggcggagcgg gcgctggaaa gtaccgaatt actggcagaa aacagtgccg atttgccgaa
    21601 agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcagcgctga atcaacaggc
    21661 gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga cgttacaggt
    21721 ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt ccaatctgct
    21781 cggtgaagcg ttgcgagcgc aggtggcacg gctaccagaa atgccaaaac cacaacagct
    21841 tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc tgcttaataa
    21901 acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg ccgagcaaaa
    21961 ccgtattctg gaagcacaac tgcgcactca gcgtgagttg ctgaactcat tgttgcaggg
    22021 tggcgacacg ctactattgg aactgaccaa gctgaaagtc tccaacgggc aactggagga
    22081 tgcgctgaaa gaggtgaacg aagcaacgca ccgctatctg ttctggacct ctgacgtgcg
    22141 tccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc tcatttcgct
    22201 ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca aagagacaat
    22261 tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc gccgctattt
    22321 cacccgtttt cttgaacgtt cggcggcgaa agttggcaaa gtgactcagg atcacttctg
    22381 gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgttac cggtgctgtg
    22441 gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg tcgcgattgg
    22501 cgatggtgta acggccaccg tgccgctgct gtgggtagtg atgatttgcg ccacctttgc
    22561 ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgcg tatctcgtgg
    22621 gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg cgctgatgat
    22681 gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggaaggcttt gctttatcct
    22741 catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga ttccgctgta
    22801 tctcaacaaa gaaggcagcg gcgacaacat taccaaccat atgctgtgga acatgatgat
    22861 tggcgcgccg ttggttgcca ttctggcgtc ggctgtgggt tatctggcaa cggcacaggc
    22921 gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg tggtttatca
    22981 cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc gggcgaagca
    23041 tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcgc atcatcacag
    23101 tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg ccatcagtgc
    23161 gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt ctgtcattgt
    23221 gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc tgtgggatgt
    23281 cacctccacg gtacagggcg tagaaagtct ggagccaatt accctcggtg cggtgctgat
    23341 tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg cgctgctgga
    23401 actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca tcaccaccat
    23461 caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga ttggtattga
    23521 gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt ttggtttgca
    23581 ggaaattttc gccaacttta tctctggtct gattatcctg ttcgaaaaac cgattcgcat
    23641 tggcgatacg gtgacaattc gcgatctcac cggtagcgtg acgaaaatta acacccgcgc
    23701 caccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg cgtttattac
    23761 cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtagtgt tgacgattcc
    23821 ggcccctgct gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg cagcgcgtcg
    23881 ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc tgcaacaagg
    23941 gattcagatt ttcgagctgc gtatttatgc cgctgagatg ggtcaccgta tgccgctacg
    24001 ccatgagatc caccagctga ttctggctgg cttccatgcc cacggtatcg atatgccatt
    24061 cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga gaacgctgac
    24121 gtctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc caaatgatgt
    24181 caacgtggct catttagcag cttaacatca tcttcagtta tcaccggccc tggagagtat
    24241 catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt tgcaggattt
    24301 catatgatca gtcgagagct aactcaggtt tattcgccat gcgactgcaa tagttctggt
    24361 aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac aatgtgctgt
    24421 cccagtctcc ggcttcaacc acaggctgaa taatggtgaa gacgttcgca aatgtcacca
    24481 ccagtacgac aaccaccatc gcaatcattg ccgacatacg cgttttgaaa atcacaaatg
    24541 gtctgtcgag atcctgacgt gctttaaaga atgggaaagc cagcgcgagg aacaggtaag
    24601 gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcg gatgcggtac
    24661 cgccaccaaa cgaaaccagc aggatgaaga cagtaaccaa cccgcactgc atccacatgg
    24721 cgatagacgg catccccatc gcattcaggc gcgtcatcgg ttccggccac aacgctttcg
    24781 gcgtcccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac gcaccggtat
    24841 aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc gacaatgacg
    24901 cttcaggtga caaatgcagt gcgttaccca gcgtcacccc gaggctcttc atcagcacat
    24961 aggtaatatt gccaaggtta acggaaccat tacttaatac ctgctgccag tttgtgctga
    25021 cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg gcaaaaacaa
    25081 tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca ccaaccgctt
    25141 caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc agaccggact
    25201 gataacccgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc ccaccattta
    25261 ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt actgcaatac
    25321 cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg acggtgacca
    25381 gaatcatcca tgctactgcc agcagaccaa ccacctgcgt aggctccagt ccggcaatac
    25441 gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc caaactttcg
    25501 cggaggtact caccatccag atgatataag aggaaaacca cataaacgta ccaatgaagg
    25561 caaaacgtgg tccgacacta ttattcatcc aggaatagat accaccttct tctttgcgat
    25621 aagcagctcc catttcagcc atcatta
//
LOCUS       DAZPJR010000047        25504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPJR010000047 DAZPJR010000000
VERSION     DAZPJR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.048"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..486
                     /gene="gspA"
                     /locus_tag="ACHVB7_001785"
     CDS             <1..486
                     /gene="gspA"
                     /locus_tag="ACHVB7_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR1913791.1"
                     /translation="ASLQTLVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQT
                     WTLTHKWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSA
                     EWRPLLVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKG
                     K"
     gene            488..907
                     /gene="gspB"
                     /locus_tag="ACHVB7_001786"
     CDS             488..907
                     /gene="gspB"
                     /locus_tag="ACHVB7_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFR1913792.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1145..1456
                     /gene="rpsJ"
                     /locus_tag="ACHVB7_001787"
     CDS             1145..1456
                     /gene="rpsJ"
                     /locus_tag="ACHVB7_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR1913793.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1489..2118
                     /gene="rplC"
                     /locus_tag="ACHVB7_001788"
     CDS             1489..2118
                     /gene="rplC"
                     /locus_tag="ACHVB7_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR1913794.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2129..2734
                     /gene="rplD"
                     /locus_tag="ACHVB7_001789"
     CDS             2129..2734
                     /gene="rplD"
                     /locus_tag="ACHVB7_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR1913795.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2731..3033
                     /gene="rplW"
                     /locus_tag="ACHVB7_001790"
     CDS             2731..3033
                     /gene="rplW"
                     /locus_tag="ACHVB7_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR1913796.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3051..3872
                     /gene="rplB"
                     /locus_tag="ACHVB7_001791"
     CDS             3051..3872
                     /gene="rplB"
                     /locus_tag="ACHVB7_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR1913797.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3889..4167
                     /gene="rpsS"
                     /locus_tag="ACHVB7_001792"
     CDS             3889..4167
                     /gene="rpsS"
                     /locus_tag="ACHVB7_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR1913798.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4182..4514
                     /gene="rplV"
                     /locus_tag="ACHVB7_001793"
     CDS             4182..4514
                     /gene="rplV"
                     /locus_tag="ACHVB7_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR1913799.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4532..5233
                     /gene="rpsC"
                     /locus_tag="ACHVB7_001794"
     CDS             4532..5233
                     /gene="rpsC"
                     /locus_tag="ACHVB7_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR1913800.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5246..5656
                     /gene="rplP"
                     /locus_tag="ACHVB7_001795"
     CDS             5246..5656
                     /gene="rplP"
                     /locus_tag="ACHVB7_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR1913801.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5656..5847
                     /gene="rpmC"
                     /locus_tag="ACHVB7_001796"
     CDS             5656..5847
                     /gene="rpmC"
                     /locus_tag="ACHVB7_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR1913802.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5847..6101
                     /gene="rpsQ"
                     /locus_tag="ACHVB7_001797"
     CDS             5847..6101
                     /gene="rpsQ"
                     /locus_tag="ACHVB7_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR1913803.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6266..6637
                     /gene="rplN"
                     /locus_tag="ACHVB7_001798"
     CDS             6266..6637
                     /gene="rplN"
                     /locus_tag="ACHVB7_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR1913804.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6648..6962
                     /gene="rplX"
                     /locus_tag="ACHVB7_001799"
     CDS             6648..6962
                     /gene="rplX"
                     /locus_tag="ACHVB7_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR1913805.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6977..7516
                     /gene="rplE"
                     /locus_tag="ACHVB7_001800"
     CDS             6977..7516
                     /gene="rplE"
                     /locus_tag="ACHVB7_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR1913806.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7531..7836
                     /gene="rpsN"
                     /locus_tag="ACHVB7_001801"
     CDS             7531..7836
                     /gene="rpsN"
                     /locus_tag="ACHVB7_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR1913807.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7870..8262
                     /gene="rpsH"
                     /locus_tag="ACHVB7_001802"
     CDS             7870..8262
                     /gene="rpsH"
                     /locus_tag="ACHVB7_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR1913808.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8275..8808
                     /gene="rplF"
                     /locus_tag="ACHVB7_001803"
     CDS             8275..8808
                     /gene="rplF"
                     /locus_tag="ACHVB7_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR1913809.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8818..9171
                     /gene="rplR"
                     /locus_tag="ACHVB7_001804"
     CDS             8818..9171
                     /gene="rplR"
                     /locus_tag="ACHVB7_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR1913810.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9186..9689
                     /gene="rpsE"
                     /locus_tag="ACHVB7_001805"
     CDS             9186..9689
                     /gene="rpsE"
                     /locus_tag="ACHVB7_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR1913811.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9693..9872
                     /gene="rpmD"
                     /locus_tag="ACHVB7_001806"
     CDS             9693..9872
                     /gene="rpmD"
                     /locus_tag="ACHVB7_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR1913812.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9876..10310
                     /gene="rplO"
                     /locus_tag="ACHVB7_001807"
     CDS             9876..10310
                     /gene="rplO"
                     /locus_tag="ACHVB7_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR1913813.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10318..11649
                     /gene="secY"
                     /locus_tag="ACHVB7_001808"
     CDS             10318..11649
                     /gene="secY"
                     /locus_tag="ACHVB7_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR1913814.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11681..11797
                     /gene="rpmJ"
                     /locus_tag="ACHVB7_001809"
     CDS             11681..11797
                     /gene="rpmJ"
                     /locus_tag="ACHVB7_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR1913815.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11944..12300
                     /gene="rpsM"
                     /locus_tag="ACHVB7_001810"
     CDS             11944..12300
                     /gene="rpsM"
                     /locus_tag="ACHVB7_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR1913816.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12317..12706
                     /gene="rpsK"
                     /locus_tag="ACHVB7_001811"
     CDS             12317..12706
                     /gene="rpsK"
                     /locus_tag="ACHVB7_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR1913817.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12740..13360
                     /gene="rpsD"
                     /locus_tag="ACHVB7_001812"
     CDS             12740..13360
                     /gene="rpsD"
                     /locus_tag="ACHVB7_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR1913818.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13386..14375
                     /gene="rpoA"
                     /locus_tag="ACHVB7_001813"
     CDS             13386..14375
                     /gene="rpoA"
                     /locus_tag="ACHVB7_001813"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR1913819.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14416..14799
                     /gene="rplQ"
                     /locus_tag="ACHVB7_001814"
     CDS             14416..14799
                     /gene="rplQ"
                     /locus_tag="ACHVB7_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR1913820.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14906..15274
                     /locus_tag="ACHVB7_001815"
     CDS             14906..15274
                     /locus_tag="ACHVB7_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR1913821.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15285..15710
                     /gene="zntR"
                     /locus_tag="ACHVB7_001816"
     CDS             15285..15710
                     /gene="zntR"
                     /locus_tag="ACHVB7_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR1913822.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15766..15984
                     /gene="arfA"
                     /locus_tag="ACHVB7_001817"
     CDS             15766..15984
                     /gene="arfA"
                     /locus_tag="ACHVB7_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR1913823.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15981..16391)
                     /gene="mscL"
                     /locus_tag="ACHVB7_001818"
     CDS             complement(15981..16391)
                     /gene="mscL"
                     /locus_tag="ACHVB7_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR1913824.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16521..17897)
                     /gene="trkA"
                     /locus_tag="ACHVB7_001819"
     CDS             complement(16521..17897)
                     /gene="trkA"
                     /locus_tag="ACHVB7_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR1913825.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17919..19208)
                     /gene="rsmB"
                     /locus_tag="ACHVB7_001820"
     CDS             complement(17919..19208)
                     /gene="rsmB"
                     /locus_tag="ACHVB7_001820"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR1913826.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19254..20201)
                     /gene="fmt"
                     /locus_tag="ACHVB7_001821"
     CDS             complement(19254..20201)
                     /gene="fmt"
                     /locus_tag="ACHVB7_001821"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR1913827.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20216..20725)
                     /gene="def"
                     /locus_tag="ACHVB7_001822"
     CDS             complement(20216..20725)
                     /gene="def"
                     /locus_tag="ACHVB7_001822"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR1913828.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20855..21979
                     /gene="dprA"
                     /locus_tag="ACHVB7_001823"
     CDS             20855..21979
                     /gene="dprA"
                     /locus_tag="ACHVB7_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR1913829.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21951..22424
                     /gene="smg"
                     /locus_tag="ACHVB7_001824"
     CDS             21951..22424
                     /gene="smg"
                     /locus_tag="ACHVB7_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR1913830.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22453..22995
                     /locus_tag="ACHVB7_001825"
     CDS             22453..22995
                     /locus_tag="ACHVB7_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR1913831.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23000..23572
                     /gene="tsaC"
                     /locus_tag="ACHVB7_001826"
     CDS             23000..23572
                     /gene="tsaC"
                     /locus_tag="ACHVB7_001826"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR1913832.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23577..24395
                     /gene="aroE"
                     /locus_tag="ACHVB7_001827"
     CDS             23577..24395
                     /gene="aroE"
                     /locus_tag="ACHVB7_001827"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR1913833.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24392..24649
                     /locus_tag="ACHVB7_001828"
     CDS             24392..24649
                     /locus_tag="ACHVB7_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR1913834.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24625..25179)
                     /locus_tag="ACHVB7_001829"
     CDS             complement(24625..25179)
                     /locus_tag="ACHVB7_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR1913835.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6492 a         6028 c         6683 g         6301 t
ORIGIN      
        1 gcttctttac aaacattggt agatcagaat ttgccctgga gcgcatcgct taaagtcggg
       61 gacaaaaaat tgcctgttgt ggtcgttcgc gtcggtgagg ctagcgttga tgtcctcgta
      121 ggtcagcaaa cgtggacgct gacacataaa tggtttgagt cggtatggac gggtgattat
      181 cttctgttgt ggaaaatgtc gccggaaggg gagagtacca ttacgcgtga tagcagcgag
      241 gaagagattc tctggctgga aacgatgtta aaccgtgcat tgcatatttc gacagaacct
      301 tcggcggagt ggcgtccctt gttggtagaa aaaataaagc aatttcaaaa aagccaccac
      361 ttgaaaactg acggtgttgt tggcttttca acgttagtac atctctggca ggtggcaggg
      421 gagagtgcct acttatatcg ggatgaagca aatatttccc cggaaacaac agtgaagggg
      481 aaatgacatg tttgaattct atatcgccgc gcgtgagcag aaagaaacag gtcaccctgg
      541 tatcttctcc cgacaaaaac acagcaccat aatatatgtc atttgtttat tgctgatttg
      601 cctgtggttt gcaggaatgg tgttggttgg tgggtatgcc aggcagctat gggcgctttg
      661 gatagtaaaa gcagaagtca ctgtagaggc tgaaacgccc gcttttaaac aatcaacaca
      721 acactatttc ttcaaaaaac agccactccc cgtcgtcgaa tccgtcgagg aggaagatga
      781 cccagacgtc gcggttgaga atgctccctc ttcttctgaa gacgaagaaa atactgtaaa
      841 agaaagcgac gagaaagccg gtttaagaga gcgtgtcaaa aatgcgctga acgaacttga
      901 gagataaccc gaaggctgtt tactttacta gcaatacgct tgcgttcggt ggttaagtat
      961 gtataatgcg cgggcttgtc gtagttgaca gcaggttcaa tctgaacctc agttagccga
     1021 atttggctac ctaacaatgc tcccaatcgg ggagctacgt aagaacggtt acactctccc
     1081 atcaatcgta atgggtctga ggagtaatca ttttcgttta taaaataatt ggagctctgg
     1141 tctcatgcag aaccaaagaa tccgtatccg cctgaaagcg tttgatcatc gtctgatcga
     1201 tcaagcaacc gcggaaatcg tcgagactgc caagcgcact ggtgcgcagg tccgtggtcc
     1261 gatcccgctg ccgacacgca aagagcgctt cactgttctg atctccccgc acgtcaacaa
     1321 agacgcgcgc gatcagtacg aaatccgtac tcacttgcgt ctggttgaca tcgttgagcc
     1381 aaccgagaaa accgttgatg ctctgatgcg tctggatctg gctgccggtg tagacgtgca
     1441 gatcagcctg ggttaatcag gtcattgagc gattgagagg ttgaaacaat gattggttta
     1501 gtcggtaaaa aagtgggtat gacccgtatc ttcacagaag acggcgtttc tatcccagta
     1561 accgtaatcg aagttgaagc aaaccgcgtt actcaggtta aagacctggc taacgatggc
     1621 taccgtgcta ttcaggtgac caccggtgct aaaaaagcta accgtgtgac caagcctgaa
     1681 gctggccact tcgctaaagc tggcgtagaa gctggccgtg gtctgtggga attccgcctg
     1741 gctgaaggcg aagagttcac tgtaggtcag agcattagcg ttgaactgtt tgctgaggtt
     1801 aaaaaagttg acgtaactgg cacctctaaa ggtaaaggtt tcgcaggtac cgttaagcgc
     1861 tggaacttcc gtacccagga cgctactcac ggtaactcct tgtctcaccg cgttccgggt
     1921 tctatcggtc agaaccagac tccgggcaaa gtgttcaaag gcaagaaaat ggcaggtcag
     1981 atgggtaacg aacgtgtaac cgttcagagc cttgacgtag tacgcgttga cgctgagcgc
     2041 aacctgctgc tggttaaagg tgctgtcccg ggtgcaaccg gtagcgacct gatcgttaaa
     2101 ccagctgtga aggcgtaagg agatagcaat ggaattagta ttgaaagacg cgcagagcgc
     2161 gctgactgtt tccgaaacta ccttcggtcg tgatttcaac gaagcgctgg ttcaccaggt
     2221 tgttgttgct tatgcagctg gtgctcgtca gggtactcgt gctcagaaga ctcgtgctga
     2281 agtaactggt tccggtaaaa aaccgtggcg ccagaaaggc accggccgtg cgcgttctgg
     2341 ttctatcaag agcccgatct ggcgttctgg tggcgtgacc tttgctgctc gtccgcagga
     2401 ccacagtcaa aaagttaaca agaagatgta ccgcggcgcg ctgaaaagca tcctgtccga
     2461 actggtacgt caggatcgtc tgatcgttgt cgagaagttc tctgtagaag cgccgaaaac
     2521 taagctgctg gcacagaaac tgaaagacat ggctctggaa gatgtgctga tcatcaccgg
     2581 tgagctggac gaaaacctgt tcctggctgc gcgcaacctg cacaaggttg acgtacgcga
     2641 tgcaactggt atcgacccgg ttagcctgat cgccttcgac aaagtcgtaa tgactgctga
     2701 tgctgttaag caagttgagg agatgctggc atgattcgtg aagaacgtct gctgaaggtg
     2761 ctgcgtgcac cgcacgtttc tgaaaaagcg tctactgcga tggaaaaatc caacaccatc
     2821 gtactcaaag ttgctaaaga cgcgaccaaa gcagaaatca aagctgctgt gcagaaactg
     2881 tttgaagtcg aagtcgaagt cgttaacacc ctggtagtta aagggaaagt taaacgtcac
     2941 ggacagcgta tcggtcgtcg tagcgactgg aaaaaagctt acgtcaccct gaaagaaggc
     3001 cagaatctgg acttcgttgg cggcgctgag taagtcggag gagtaataca atggcagttg
     3061 ttaaatgtaa accgacatct ccgggtcgtc gccacgtagt taaagtggtt aaccctgagc
     3121 tgcacaaggg caaacctttt gctccgttgc tggaaaaaaa cagcaaatcc ggtggtcgta
     3181 acaacaatgg ccgtatcacc actcgtcata tcggtggtgg ccacaagcag gcttaccgta
     3241 ttgttgactt caaacgcaac aaagacggta tcccggcagt tgttgaacgt cttgagtacg
     3301 atccgaaccg ttccgcgaac atcgcgctgg ttctgtacaa agacggtgaa cgccgttaca
     3361 tcctggcccc taaaggcctg aaagctggcg accagattca gtctggcgtt gatgctgcaa
     3421 tcaaaccagg taacaccctg ccgatgcgca acatcccggt tggttctact gttcataacg
     3481 tagaaatgaa accaggtaaa ggcggtcagc tggcacgttc cgctggtact tacgttcaga
     3541 tcgttgctcg tgatggtgct tatgtcaccc tgcgtctgcg ttctggtgaa atgcgtaaag
     3601 tagaagcaga ctgccgtgca actctgggcg aagttggcaa tgctgagcat atgctgcgcg
     3661 ttctgggtaa agcaggtgct gcacgctggc gtggtgttcg tccgaccgtt cgcggtaccg
     3721 cgatgaaccc ggtagaccac ccacatggtg gtggtgaagg tcgtaacttt ggtaagcacc
     3781 cggtaactcc gtggggcgtt cagaccaaag gtaagaagac ccgcagcaac aagcgtactg
     3841 ataaattcat cgtacgtcgc cgtagcaaat aattttagag gataagccat gccacgttct
     3901 ctcaagaaag gtccttttat tgacctgcac ttgctgaaga aggtagagaa agcggtggaa
     3961 agcggagaca agaagcccct gcgcacttgg tcccgtcgtt caacgatctt tcctaacatg
     4021 atcggtttga ccatcgctgt ccataatggt cgtcagcacg ttccggtatt tgtgaccgac
     4081 gaaatggtcg gtcacaaact gggtgaattc gcaccgactc gtacttatcg cggccacgct
     4141 gctgataaaa aagcgaagaa gaaataaggt aggaggaaga gatggaaact atcgctaaac
     4201 atcgccatgc tcgttcttct gctcagaagg ttcgccttgt tgctgacctg attcgcggta
     4261 agaaagtgtc gcaggctctg gatattttga cctacaccaa caagaaagcg gctgtactgg
     4321 tcaagaaagt tctggaatct gccattgcta acgctgaaca caacgatggc gctgacattg
     4381 acgatctgaa agttacgaaa attttcgtag acgaaggccc gagcatgaag cgcattatgc
     4441 cgcgtgcaaa aggtcgtgca gatcgcatcc tgaagcgcac cagccacatc actgtggttg
     4501 tgtccgatcg ctgagactct ggagactagc aatgggtcag aaagtacatc ctaatggtat
     4561 tcgcttgggt attgtaaaac catggaactc tacctggttt gcgaacacca aagaattcgc
     4621 tgacaacctg gacagcgatt ttaaagtacg tcagtacctg actaaggaac tggctaaagc
     4681 gtctgtatct cgtatcgtta tcgagcgtcc ggctaagagc atccgtgtaa ccattcacac
     4741 tgctcgcccg ggtatcgtta tcggtaaaaa aggtgaagac gtagaaaaac tgcgtaaggt
     4801 cgtagcggac atcgctggcg ttcctgcaca gatcaacatc gccgaagttc gtaagcctga
     4861 actggacgca aaactggttg ctgacagcat cacttctcag ctggaacgtc gcgttatgtt
     4921 ccgtcgtgct atgaagcgtg ctgtacagaa cgcaatgcgt ctgggcgcta aaggtattaa
     4981 agttgaagtt agcggccgtc tgggcggcgc ggaaatcgca cgtaccgaat ggtaccgcga
     5041 aggtcgcgta ccgctgcaca ctctgcgtgc tgacatcgac tacaacacct ctgaagcgca
     5101 caccacttac ggtgtaatcg gcgttaaagt gtggatcttc aaaggcgaga tcctgggtgg
     5161 tatggctgct gttgaacaac cggaaaaacc ggctgctcag cctaaaaagc agcagcgtaa
     5221 aggccgtaaa taaggagcgt cgctgatgtt acaaccaaag cgtacaaaat tccgtaaaat
     5281 gcacaaaggc cgtaaccgcg gtctggcgca gggtacggat gttagcttcg gcagcttcgg
     5341 tctgaaagct gttggccgtg gtcgtctgac tgcccgtcag atcgaagcag cacgtcgtgc
     5401 tatgacccgt gcagttaagc gtcaaggtaa gatctggatc cgtgtgttcc cggacaaacc
     5461 gatcactgaa aagccgctgg cagtgcgtat gggtaaaggt aaaggtaacg tggagtattg
     5521 ggttgccttg attcagccgg gtaaagtcct gtatgaaatg gacggtgttc cggaagagct
     5581 ggcccgtgaa gcattcaagc tggcagcagc gaaactgccg attaaaacca cctttgtaac
     5641 taagacggtg atgtaatgaa agcaaaagag ctgcgtgaga agagcgttga agagctgaac
     5701 accgagctgc tgaacctgct gcgtgagcag ttcaacctgc gtatgcaggc tgcaagtggc
     5761 cagctgcaac agtctcacct gttgaagcaa gtgcgtcgcg atgtcgcacg cgttaagact
     5821 ttactgaacg agaaggcggg tgcgtaatga ccgataaaat ccgtactctg caaggtcgcg
     5881 ttgttagcga caaaatggag aaatccattg ttgttgctat cgaacgtttt gtgaaacacc
     5941 cgatctacgg taaattcatc aagcgtacga ccaaactgca cgtacatgac gagaacaacg
     6001 aatgcggtat cggtgacgtg gttgaaatcc gcgaatgccg tccgctgtcc aagactaaat
     6061 cctggacgct ggttcgcgtt gtagagaaag cggttctgta atacagtaca ctctctcgat
     6121 acgaataaac ggctcagaaa tgagccgttt attttttcta cccatatcct tgaagcggtg
     6181 ttataatgcc gcgccctcga tatggggatt tttaacgacc tgattttcgg gtctcagtag
     6241 tagttgacat tagcggagca ctaaaatgat ccaagaacag actatgctga acgtcgccga
     6301 caactccggt gcacgtcgcg taatgtgtat caaggttctg ggtggctcgc accgtcgcta
     6361 cgcaggcgta ggcgacatca tcaagatcac catcaaagaa gcaattccgc gtggtaaggt
     6421 caaaaaaggt gatgtgctga aggcggtagt ggtgcgcacc aagaagggtg ttcgtcgccc
     6481 ggacggttct gtcattcgct tcgatggtaa tgcttgtgtt cttctgaaca acaacagcga
     6541 gcagcctatc ggtacgcgta tttttgggcc ggtaactcgt gagcttcgta gtgagaagtt
     6601 catgaaaatt atctctctgg caccagaagt actctaagga gcgaatcatg gcagcgaaaa
     6661 tccgtcgtga tgacgaagtt atcgtgttaa ccggtaaaga taaaggtaaa cgcggtaaag
     6721 ttaagaatgt cctgtcttcc ggcaaggtca ttgttgaagg tatcaacctg gttaagaaac
     6781 atcagaagcc ggttccggcc ctgaaccaac cgggtggcat cgttgaaaaa gaagccgcta
     6841 ttcaggtttc caacgtagca atcttcaatg cggcaaccgg caaggctgac cgtgtaggct
     6901 ttagattcga agacggcaaa aaagtccgtt tcttcaagtc taacagcgaa actatcaagt
     6961 aatttggagt agtacgatgg cgaaactgca tgattactac aaagacgaag tagttaaaaa
     7021 actcatgact gagtttaact acaattctgt catgcaagtc cctcgggtcg agaagatcac
     7081 cctgaacatg ggtgttggtg aagcgatcgc tgacaaaaaa ctgctggata acgcagcagc
     7141 agacctggca gcaatctccg gtcaaaaacc gctgatcacc aaagcacgca aatctgttgc
     7201 aggcttcaaa atccgtcagg gctatccgat cggctgtaaa gtaactctgc gtggcgaacg
     7261 catgtgggag ttctttgagc gcctgatcac tattgctgta cctcgtattc gtgacttccg
     7321 tggcctgtcc gctaagtctt tcgacggtcg tggtaactac agcatgggtg tccgtgagca
     7381 gatcatcttc ccagaaatcg actacgataa agtcgaccgc gttcgtggtt tggatattac
     7441 cattaccact actgcgaaat ctgacgaaga aggccgcgct ctgctggctg cctttgactt
     7501 cccgttccgc aagtaaggta gggttactaa atggctaagc aatcaatgaa agcacgcgaa
     7561 gtaaaacgcg tagctttagc tgataaatac ttcgcgaaac gcgctgaact gaaagcgatc
     7621 atctctgatg tgaacgcttc cgacgaagat cgttggaacg ctgttctcaa gctgcagact
     7681 ctgccgcgtg attccagccc gtctcgtcag cgtaaccgct gccgtcaaac aggtcgcccg
     7741 catggtttcc tgcggaagtt cgggttgagc cgtattaagg tccgtgaagc cgctatgcgc
     7801 ggtgaaatcc cgggtctgaa aaaggctagc tggtaattgt caccaattga atcacgggag
     7861 taaagacaga tgagcatgca agatccgatc gcggatatgc tgacccgtat ccgtaacggt
     7921 caggccgcga acaaagctgc ggtcaccatg ccttcctcca agctgaaagt ggcaatcgcc
     7981 aacgtgctga aggaagaagg ttttattgaa gattttaaag ttgaaggcga caccaagcct
     8041 gaactggaac ttactctgaa gtatttccag ggcaaagctg ttgtagaaag cattcagcgt
     8101 gtcagccgcc caggtctgcg catctataaa cgtaaagatg agctgccgaa agttatggcg
     8161 ggtctgggta tcgcagttgt ttctacctct aaaggtgtta tgactgatcg tgcagcgcgc
     8221 caggctggtc ttggtggcga aattatctgc tacgtagcct aatcggagga aaaaatgtct
     8281 cgtgttgcta aagcaccggt cgttgttcct gccggcgttg acgtaaaaat caacggtcag
     8341 gttattacga tcaaaggtaa aaacggcgag ctgactcgta ctctcaacga tgctgttgaa
     8401 gttaaacatg cagataatac cctgaccttc ggtccgcgtg atggttacgc agacggttgg
     8461 gcacaggctg gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt taccgaaggc
     8521 ttcactaaga agctgcagct ggttggtgta ggttaccgtg cagcggttaa aggcaatgtg
     8581 attaacctgt ctctgggttt ctctcatcct gttgaccatc agctgcctgc gggtatcact
     8641 gctgaatgtc cgactcagac tgaaatcgtg ctgaaaggcg ttgataagca ggtgatcggc
     8701 caggttgcag cggatctgcg cgcctaccgt cgtcctgagc cttataaagg caagggtgtt
     8761 cgttacgccg acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg taacactatg
     8821 gataagaaat ttgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct ccaggagctg
     8881 ggcgcaactc gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca ggtaattgca
     8941 ccgaacggtt ctgaagttct ggtagctgct tctactgtag aaaaagctat cgctgaacaa
     9001 ctgaagtaca ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt cgctgaacgc
     9061 gctctggaaa aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca atatcatggt
     9121 cgtgtccagg cactggcaga tgctgcccgt gaagctggcc ttcagttcta aggtagaggt
     9181 gtaagatggc tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg atcgcggtaa
     9241 accgcgtatc taaaaccgtt aaaggtggtc gtattttctc cttcacagct ctgactgtag
     9301 ttggcgatgg taacggtcgc gttggttttg gttacggtaa agcgcgtgaa gttccagcag
     9361 cgatccagaa agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg ctgaataacg
     9421 gcactctgca acaccctgtt aaaggtgttc acacgggttc tcgcgtattc atgcagccgg
     9481 cttccgaagg taccggtatc atcgccggtg gtgcaatgcg cgccgttctg gaagtcgctg
     9541 gggttcataa cgttctggct aaagcatatg gttccaccaa cccgatcaac gtggttcgtg
     9601 caactattga tggcctggaa aatatgaatt ctccagaaat ggtcgctgcc aagcgtggta
     9661 aatccgttga agaaattctg gggaaataaa ccatggcaaa gactattaaa attactcaaa
     9721 cccgcagtgc aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc ctgggtctgc
     9781 gtcgtattgg tcacaccgta gagcgcgagg atactcctgc tattcgcggt atgatcaacg
     9841 cggtttcctt catggttaaa gttgaggagt aagagatgcg tttaaatact ctgtctccgg
     9901 ccgaaggctc caaaaaggcg ggtaaacgcc tgggtcgtgg tatcggttct ggcctcggta
     9961 aaaccggtgg tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc gtacgtcgcg
    10021 gtttcgaggg tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc ttcacttctc
    10081 gtaaagcagc gattacagcc gaagttcgtc tgtctgacct ggctaaagta gaaggcggtg
    10141 ttgtagacct gaacacgctg aaagcggcta acattatcgg tatccagatc gagttcgcga
    10201 aagtgatcct ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg cgtgttacta
    10261 aaggcgctcg tgctgctatc gaagctgctg gcggtaaaat cgaggaataa gtagcagatg
    10321 gctaaacaac cgggattaga ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc
    10381 agactgctgt ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt tattccgatc
    10441 cctggtattg atgccgctgt acttgccaaa ctgcttgagc aacagcgagg caccatcatt
    10501 gagatgttta acatgttctc tggtggtgct ctcagccgtg cttctatctt tgctctgggg
    10561 atcatgccgt atatttcggc gtcgatcatt atccagctgc tgacggtggt tcacccaacg
    10621 ttggcggaaa ttaagaaaga aggggagtct ggtcgtcgta agatcagcca gtacacccgc
    10681 tacggtactc tggtgctggc aatattccag tcgatcggta ttgctactgg tctgccgaat
    10741 atgcctggta tgcaaggcct ggtgattaac ccgggctttg cattctactt caccgctgtt
    10801 gtaagtctgg tcacaggaac gatgttcctg atgtggttgg gcgaacagat tactgaacga
    10861 ggtatcggca acggtatttc aatcattatc ttcgtcggta ttgtcgcggg actcccgcca
    10921 gccattgccc atactatcga gcaagcgcgt caaggcgacc tgcacttcct cgtgttgctg
    10981 ttggttgcag tattagtatt tgcagtgacg ttctttgttg tatttgttga gcgtggtcaa
    11041 cgccgcattg tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgttta tgctgcacag
    11101 agcacacatt taccgctgaa agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc
    11161 agtattattc tgttcccggc gaccatcgcg tcatggttcg ggggcggtac tggttggaac
    11221 tggctgacaa caatttcgct gtatttgcag cctgggcaac cgctttatgt gttactctat
    11281 gcgtctgcaa tcatcttctt ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa
    11341 acagcagata acctgaagaa gtccggtgca tttgtaccag gaattcgtcc gggagagcaa
    11401 acggcgaagt atatcgataa agtaatgacc cgcctgaccc tggttggtgc gctgtatatt
    11461 acctttatct gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc
    11521 ggtgggacct cactgcttat cgttgttgtc gtgattatgg actttatggc tcaagtgcaa
    11581 actctgatga tgtccagtca gtatgagtct gcattgaaga aggcgaacct gaaaggctac
    11641 ggccgataat tggtcgcccg agaagttacg gagagtaaaa atgaaagttc gtgcttccgt
    11701 caagaaatta tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg
    11761 cagtgccgag ccgaagcata aacagcgcca aggctgattt tttcgcatat ttttcttgca
    11821 aagttgggtt gagctggcta gattagtcag ccaatctttt gtatgtctgt gcgtttccat
    11881 ttgagtatcc tgaaaacggg cttttcagca tggaacgtac atattaaata gtaggagtgc
    11941 atagtggccc gtatagcagg cattaacatt cctgatcata agcatgccgt aatcgcatta
    12001 acttcgattt atggcgtcgg caagacccgt tctaaagcca tcctggctgc agcgggtatc
    12061 gctgaagatg ttaagatcag tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa
    12121 gttgccaaat ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc
    12181 ctgatggatc ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt
    12241 cagcgtacca agaccaacgc acgtacccgt aagggtccgc gcaaaccgat caagaaataa
    12301 tcggggtgat tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca
    12361 agtctctgac ggcgtggctc atatccatgc ttctttcaac aacaccatcg tgactatcac
    12421 tgatcgtcag ggtaacgcgt tgggttgggc aacagccggt ggttccggtt tccgtggttc
    12481 tcgcaaatcc actccgtttg cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa
    12541 agaatacggc atcaagaatc tggaagttat ggttaaaggt ccgggtccag gccgcgaatc
    12601 tactattcgt gctctgaacg ccgcaggttt ccgcatcact aacattactg atgtgactcc
    12661 gatccctcat aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc tcgttttcca
    12721 ggtttgttgg agaaagaaaa tggcaagata tttgggtcct aagctcaagc tgagccgtcg
    12781 tgagggcacc gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat
    12841 tgaacaagct cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca
    12901 gttgcgtgaa aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa
    12961 ctactacaaa gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct
    13021 ggaaggtcgt ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc
    13081 acgtcagctg gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc
    13141 ttatcaggtt agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg
    13201 cgtgaaagcc gctctggagc tggctgagca gcgtgaaaag ccaacctggc tggaagttga
    13261 tgctggcaag atggaaggta cgtttaagcg taagccggag cgttctgatc tgtctgcgga
    13321 cattaacgaa cacctgatcg tcgagcttta ctccaagtaa agcttagtac caaagagagg
    13381 acacaatgca gggttctgtg acagagtttc taaaaccgcg cctggttgat atcgagcaag
    13441 tgagttcgac gcacgccaag gtgacccttg agcctttaga gcgtggcttt ggccatactc
    13501 tgggtaacgc actgcgccgt attctgctct catcgatgcc gggttgcgcg gtgaccgagg
    13561 ttgagattga tggtgtacta catgagtaca gcaccaaaga aggcgttcag gaagatatcc
    13621 tggaaatcct gctcaacctg aaagggctgg cggtgagagt tcagggcaaa gatgaagtta
    13681 ttcttacctt gaataaatct ggcattggcc ctgtgactgc agccgatatc acccacgacg
    13741 gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt
    13801 ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa
    13861 ttcattcgga agaagatgag cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc
    13921 ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt agaacagcgt accgacctgg
    13981 acaagctggt catcgaaatg gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc
    14041 gtgcggcaac cattctggct gaacaactgg aagctttcgt tgacttacgt gatgtacgtc
    14101 agcctgaagt gaaagaagag aaaccagagt tcgatccgat cctgctgcgc cctgttgacg
    14161 atctggaatt gactgtccgc tctgctaact gccttaaagc agaagctatc cactatatcg
    14221 gtgatctggt acagcgtacc gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat
    14281 ctcttactga gattaaagac gtgctggctt cccgtggact gtctctgggc atgcgcctgg
    14341 aaaactggcc accggcaagc atcgctgacg agtaaccgga tcacaggtta aggttttact
    14401 gagaaggata aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg caacagcagc
    14461 catcgccagg ctatgttccg caatatggca ggttcactgg ttcgtcatga aatcatcaag
    14521 acgactctgc ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat tactcttgcc
    14581 aagactgata gcgttgctaa tcgtcgtctg gcatttgccc gtactcgtga taacgagatc
    14641 gtggcaaaac tgtttaacga actgggcccg cgtttcgcga gccgtgccgg tggttacact
    14701 cgtattctga agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta catcgagctg
    14761 gttgatcgtt cagagaaagc agaagctgct gcagagtaat ctgaagcaat gtaaaaaaac
    14821 ccgccccggc gggttttttt atacccgtag tatccccact tatctacaat agctgtactc
    14881 tttttgttca tcccctggag tatttatgtg gttacttgac cagtgggcag agcgccatat
    14941 agcagaagcg caagcgaaag gtgagtttga taacctggca ggtagcggcg aaccattgat
    15001 actggatgat gattctcacg tgccaccgga attacgtgcg gggtatcgct tgctgaagaa
    15061 tgccggttgc ttaccgccag aacttgagca acggagagaa gcaattcagc ttctggatat
    15121 tctcaaaggt atccgtcacg atgatccgca atatcaagag gttagccgtc gattgtcatt
    15181 actggaattg aagctgcgac aagctggatt gagtaccgat tttttacgcg gcgattatgc
    15241 tgacaagttg ttggacaaaa tcaacgataa ctagtggagt atgtatgtat cgcattggtg
    15301 agctggcaaa aatggcggaa gtaacacctg acacgattcg ttattacgaa aaacagcaga
    15361 tgatggagca tgaagtgcgt actgaaggcg ggtttcgcct gtataccgaa agcgatctcc
    15421 agcgattgaa atttatccgc catgccagac aactaggttt cagtctggag tcgatccgcg
    15481 agttgctgtc gatccgcatc gatcctgaac accatacctg tcaggagtca aaaggcattg
    15541 tgcaggaaag attgcaggaa gtcgaagcac ggatagccga gttgcagagt atgcagcgtt
    15601 ccttgcaacg ccttaacgat gcctgttgtg ggaccgccca tagcagtgtt tattgctcga
    15661 ttcttgaagc tcttgaacaa ggggcgagtg gcgttaagag tggctgttga ttttttgcac
    15721 tgacaggatt acactcgcgc cgttaaataa ccaactggag tttttatgag ccgatatcag
    15781 catactaaag ggcagataaa ggataatgcg atagaagcat tactacatga tcccttattc
    15841 cgacagcgag tagagaaaaa taagaaaggg aaaggcagtt acatgcgaaa aggaaaacat
    15901 ggcaatcggg ggaactggga ggccagtggc aagaaagtaa atcacttttt taccactggt
    15961 cttctgcttt caggcgcttg ttaagagcgg ttattctgct ctttcagcaa atcacgaatt
    16021 tctgccagta atacttcttc tttagttggt gcaggtgcgg cagctggttc ttcttttttc
    16081 cgattcagtt tgttgattag cttaatcgcc ataaagatgg caaaggccac aatcagaaaa
    16141 tcaaagacgt tttgaatgaa gacaccgtaa tgcatcacaa cagcagggat atccccctgc
    16201 gcatcgcgta gcgtgacagc aaactgttta aagtcgatcc cgccaattaa taagcccaga
    16261 ggtggcatga tgatatcggc aaccagtgaa gatacaatct tcccgaatgc cgcaccgata
    16321 atgacaccca ccgccaaatc caccacgttc ccgcgcatcg caaattcgcg aaattcttta
    16381 ataatgctca tgttattctc cctatgaagc cgacaaccat aagtctaaca aatgttaagc
    16441 cattttcctg ccaggaatga atttaattaa tccttgataa tcaaagggaa ataataaggc
    16501 gtcattagac gccttattaa ttacaagaag aaagggcttg gctggaagag tctttcgacg
    16561 tcggtaataa actttttatc tgtgaggaac ataattacgt gatcgccttg ctcaatgcgc
    16621 agattgtcat tggcaatcat cacgtcgttt ccacgtacca ccgctccaat aatcgttcct
    16681 ggcggtagct tgatttcgtc aatgactctg ccgacaacgc gtgaggtgct ttcatcaccg
    16741 tgagcaacgg cttcaatagc ttctgctacg ccgcggcgca atgaggaaac accaacaata
    16801 tctgcttttc gcacatggct aagcaacgca gaaatagttg cttgttgtgg tgaaattgca
    16861 atatcgatca cgctcccctg aaccagatcc acataagcgc gacgctggat caataccatc
    16921 acctttttcg cacccatacg tttggcaagc atggcggaca tgatattggc ctcgtcatcg
    16981 ttggtgacgg caataaacag atcaacttga tcgatatgtt cttcggccag cagttcttga
    17041 tccgacgcat caccaaaaaa gacgatcgta ttctgcaact tttccgccag ttcggcagcg
    17101 cgctgctgat tacgttcgat gagtttaacg ctgtaatctt tttccagacg acgcgccagc
    17161 cctgcaccga tattaccgcc accaaccagc atgatccgct tatacggttt ttccagtcgc
    17221 tgtaattcac tcatcaccgc gcggatatgc tgtgaagcgg caataaagaa cacttcatca
    17281 ccagcttcaa caatggtcga accttgtgga cgaatggggc gatcgtggcg gaaaattgct
    17341 gccacacgag tatcgatatg tggcatatgt tcgcgcatgg tcgaaagtgc attaccaatc
    17401 agcgggccgc cataataggc tttaactaca gcgaggctga ctttaccctc agcgaagttc
    17461 accacctgca atgcgcctgg atactcaatc agtcggtaaa tattatcgat aaccaactgc
    17521 tctggtgcga tcagatgatc aatcggtaca gcatctgaat gaaatagctt atcggcatcg
    17581 cgtacgtagt ctggtgagcg gatgcgagcg atgcgattag gggtgttgaa aagtgagtag
    17641 gctacctggc aggcaaccat attggtttca tctgaactgg ttacagcaac cagcatatcg
    17701 gcgtcgtcgg cacctgcctc ccgcaatacg cgtggatgag agccatgccc ctgcacgact
    17761 cgcaggtcaa atttatcctg caaggtccgc agacgctcac cgttggtatc gacaacagta
    17821 atatcgttgt tctcgccaac caggttttcc gccagtgtgc cgccaacctg gccggcaccc
    17881 agaatgataa ttttcatcag tctcgacccg ttatctcatc actttttgat tagcttagcg
    17941 taaaagaagc cgtcgccctc ttcggcacca ggcagatttt gtttacccgg ttgctctggt
    18001 gttcctgttt cgcaaagttc ggcatcagcg gtacgttgca aaaaagcttt aatctgcagg
    18061 ctattctctt ccggtaacac cgaacaggtg gcatagacca gggttccacc ggattttaaa
    18121 tgcggccaaa tggcgtcgag aatttcagac tgcaattgcg cgagttccgg gatatcgcga
    18181 tcgcgacgta accatttaat atccggatgg cgacgaatca cccccgttgc tgaacaaggc
    18241 gcatctaata aaatgcgatc aaactgttgc tcgccacacc attgggaagg gtaacggcca
    18301 tcaccttgtt tcacggtcgc cttcatacca aggcgtttta aattgtcgtg aacgcgagag
    18361 aggcgctgtt cgtcaatatc aaccgcaaca acctgcgctt ctggtgccac ctcaaggata
    18421 tgcgttgttt taccgccggg ggcggcacaa agatccagaa tgtgttcacc gttttgtggc
    18481 gcaagccagg tcatgcaacc ttgtgctgat gcatcctgaa cggtaaccca tccgtcttca
    18541 aaaccaggta gcgcatgaac aggtgcaggt gtttccagcc gtacagcatc agggtaatcc
    18601 gcatgcggga aacctttcat tcctgcttca tccaacaatg caagccagct gtcgcgggaa
    18661 tgatgcgtac gattaacgcg cagccacatt ggcggacgct ggttattggc ttcgacgatg
    18721 gattgccact gctctggata cgctttttgc agacgcttca gcaaccagga aggatgcaga
    18781 taacgtgcat cactggtatt aaactcggct aataactctt cttgctgacg ctggaactgg
    18841 cgtaatacac cgttaatcaa ccctttaagt tgcggacgct taattgcgat agcgccttca
    18901 accgtttcag ccagcgcagc atgaggtgga atgcgggtat aaagcagttg atacaaacca
    18961 accataatca ggtaatgcac agtgcgctgt ttgccggtca tcggacgggc cattaactta
    19021 ttaatcagcc agtctaactg cgaaagcgta cgcagtacgc caaagcacaa ctcttgaaga
    19081 agtgctttgt ctttatcaga aactttttgc tggagcggtg gcagaatgtt gcttaatgat
    19141 tgcccttgct cgacgacttg ttcaacggcc tgggccgcca tgctacgtaa attacgttgt
    19201 tttttcataa gtataaaaat gcccggtaag accgggctta gaagagtgga ctatcaggcc
    19261 agacggttgc ccggaacaaa ccattcccga cgagaattca ggaggtcttg cgcgctcatc
    19321 gctttcttac ccgcaggttg taacgagagc aggttcagga tgccatcacc agtcgcaacc
    19381 tgaatgcctt gtttgttggc ttcaaggatc gttcctggtg cagcgttggt tgccgtatca
    19441 atgaccgacg ctttccagac tttaaccggt tgtccttcaa tttccagcca gctcattggc
    19501 catgggttga aagcgcgaat gcagcgttca agctgcgctg ccgaaagcga ccagtcaata
    19561 cgcgcttctt ctttactcaa cttctcggcg taagtgacaa gagtttcgtc ctgaacttct
    19621 ggtttcgccg tgccgtctgc tagttgtttc aacgtggtga ttagcccttg tgggccaagc
    19681 tctgccagct tgtcgtacag cgtaccactg gtatcttctg cagtaatcgg gcaggagagc
    19741 ttatagagca tatcaccggt gtctaaaccg acatccattt gcataatggt cacaccagtt
    19801 tctgcatcac ccgcccatag tgagcgttgg attggtgcag caccgcgcca gcgtggcagc
    19861 agtgaaccat gaacgttgat acagccaaga cgcggcatct ccagcactgc tttaggcaga
    19921 attaagccat aggcgacgac gaccataaca tcagcctgca gatcggcgac cagttgctgg
    19981 ctttcttgtg gacgcaggga aacaggttga aaaacgggca gacctttttc ctcagccaga
    20041 actttaaccg ggctgggcat cagtttttta ccgcgtcctg ccggtcggtc tggctgggtg
    20101 aacacgccaa cgacgttatg accagaagac aacagcgcgt cgagatgacg cgctgcaaag
    20161 tcaggtgtac ccgcaaaaat aatacgtagt gattctgaca cgttagttct tatccttaag
    20221 cccgggcttt cagacgatct agtttttcaa ctttctgacg aatacgttgt tgtttcagcg
    20281 gtgacagata atccataaac agtttgccga ccaggtgatc catctcatgc tgaatacaga
    20341 tggctaacag cccctctgct tccagttcaa atggtttacc gtcgcggtca agggcgcgaa
    20401 ttttaacttt ctctgcgcgc ggcactaaag cacgttgttc agggatcgac aggcaacctt
    20461 cttcaatgcc tgtttcgccg cttttttcta aaagctccgg attgattaac accagccgtt
    20521 cgtcacggtt ttccgaaaca tcaataacaa tgatacgttg atggatatca acctgggttg
    20581 ccgccaggcc aataccttct tctgcgtaca tcgtctcgaa catatcatcg acgatacgct
    20641 gtatttctgc attcacttct tctaccggtt tagcaacttt gcgaagccgc tcgtccggaa
    20701 tatgtaacac ttgcaaaact gacataaatc tccagagatg tgttcagggg ttagaaagat
    20761 tatttcttct attctagaca aatccccctc tgattgacag catcactgac caatcgcaaa
    20821 gattgctaag gctgcttatg gcagggagat aaggatggtc gatacagaaa tttggctgcg
    20881 tttaatgagt atcagcagct tgtacggcga tgatatggtc cgtatagctc actggctggc
    20941 aaaacagtcg caaattgatg cggttggatt gcagcaaaca gggcttacat tgcggcaggc
    21001 acaacgcttt ctttcatttc cgcgaaagag tatcgaaagc tcactttgtt ggttggagca
    21061 acccaaccat catttaatcc ctgcggacag cgaattttat cctcctcaac ttctggtgac
    21121 gacagattac cccggcgcac tgtttgttga aggagaactg cacgcgctgc attcatttca
    21181 gcttgccgta gtggggagtc gggcgcattc atggtatggc gagcgatggg gacggttatt
    21241 ttgcgaaact ctggcgacgc gtggagtgac aattacgagt ggactggcgc gtggaatcga
    21301 tggtgtggcg cataaagctg ccttacaggt aaatggcgtc agcattgctg tattggggaa
    21361 tggacttaat accattcatc cccgccgcca tgcccgactg gctgccagtc tgcttgaaca
    21421 tggcggagct ctcgtctcgg aatttcccct cgatgttcca ccccttgctt acaatttccc
    21481 acgaagaaat cgcattatca gtggtctaag taaaggtgta ctggtggtgg aagcggcttt
    21541 gcgcagtggt tcgctggtga cagcacgttg tgcgcttgag cagaggcgtg aagtttttgc
    21601 cttgccaggt ccaataggga atccgggaag cgaagggcct cactggttaa taaaacaagg
    21661 tgcgattctt gtgacggaac cggaagaaat tctggaaaac ttgcaatttg gattgcactg
    21721 gttgccagac gcccctgaaa attcatttta ttcaccagat cagcaagacg tggcattgcc
    21781 atttcctgag ctcctggcta acgtaggaga tgaggtaaca cctgttgacg tcgtcgctga
    21841 acgtgccggc caacctgtgc cagaggtagt tactcaacta ctcgaactgg agttagcagg
    21901 atggatcgca gctgtacccg gcggctatgt ccgattgagg agggcatgcc atgttcgacg
    21961 tactaatgta tttgtttgaa acctatattc acactgaagc tgagttgcgt gtggatcaag
    22021 acaaacttga acaggatctt accgacgcag gttttgagcg agaagatatc tacaatgccc
    22081 tgctatggct ggaaaaactt gctgattatc aggaagggtt ggcagaaccg atgcaactgg
    22141 cctcggatcc tctctccatg cgtatttata caccggaaga gtgtgaacga ctggatgcca
    22201 gctgccgtgg gtttctgctt ttccttgagc agattcaggt gctcaacctt gaaactcgtg
    22261 aaatggtgat agagcgagtg ctcgcgctgg ataacgcaga gttcgaactg gatgatctga
    22321 aatgggtgat cctgatggtg ttgttcaaca tcccggggtg cgaaaatgcg taccagcaaa
    22381 tggaagaatt actctttgaa gtgaatgaag gtatgctgca ttaatcattt ctttaattca
    22441 gcataagttg ttatggcaaa atcagcactg ttcacggtgc gtaataatga gtcctgccca
    22501 aagtgcgggg ctgaactggt tattcgatcc gggaaacacg gtccgtttct tggatgctca
    22561 cagtatccgg cgtgtgacta cgtccgtctt ctgaaatctt cagcggatgg acatattgtc
    22621 aaagttctgg aggggcaggt ttgccctgca tgtggcgcaa atctggtatt acgccaggga
    22681 cgctttggta tgtttattgg ttgcagtaac taccctgaat gcgaacatac cgaacttatc
    22741 gataaaccgg acgaaacagc aattacatgc ccccaatgtc ggacgggcca tctggtccag
    22801 cgccgctccc gttatggcaa aacatttcac tcctgtgatc gctacccgga gtgtcaattt
    22861 gccattaact tcaaaccaat agctggagaa tgccctgagt gtcattatcc gctactcatc
    22921 gaaaagaaaa ccgcgcaggg tgtaaaacac ttttgtgcca gtaaacaatg tggaaagccg
    22981 gtttcggcgg aataataacg tgaataataa cctgcaagga gacgctatcg cagctgcgat
    23041 agatgtcctc aatgaagaac gtgtcatcgc ctatccaacg gaagccgttt tcggtgttgg
    23101 gtgcgatcct gatagcgaaa cagcagtgat gagactgttg gagttaaaac agcgtccggt
    23161 tgataagggg ctgattttaa tcgcggcaaa ttacgagcag cttaaaccct atattgatga
    23221 caccatgctg actgatgcgc agcgtgaaac cattttttcc cgctggccag gtcctgtcac
    23281 ctttgtcttt cccgcgcctg cgacaacacc gcgctggttg acgggccgct ttgattcgct
    23341 tgctgtacga gtcaccgacc atccgttggt ggttgctttg tgccaggctt atggtaaacc
    23401 gctggtttct accagtgcca acttgagtgg attgccacct tgtcgaacag tagacgaagt
    23461 tcgcgcacaa tttggcgcgg cgttcccggt tgtgcctggt gaaacggggg ggcgtttaaa
    23521 tccttcagaa atccgcgatg ccctgacggg tgaactgttt cgacaggggt aacataatgg
    23581 aaacctatgc tgtttttggt aatccgatag cccacagcaa atcgccattc attcatcagc
    23641 aatttgctca gcaactgaat attgaacatc cctatgggcg cgtgttggca cccatcaatg
    23701 atttcatcaa cacactgaac gctttctttc gtgctggtgg taaaggtgcg aatgtgacgg
    23761 tgccttttaa agaagaggct tttgccagag cggatgagct tactgaacgg gcagcgttgg
    23821 ctggtgcagt taataccctc aagcggttag aagatggacg cctgctgggt gacaataccg
    23881 atggtgtagg cttgttaagc gatctggaac gtctgtcttt tatccgccct ggtttacgta
    23941 ttctgcttat cggcgctggt ggagcatctc gcggcgtact actgccactt ctttccctag
    24001 actgtgcggt gacaatcact aatcggacgg tatcccgcgc ggaagagttg gctaaattgt
    24061 ttgcgcacac tggcagtatt caggcgttgg gtatggacga actggaaggt catgagtttg
    24121 atctcattat taatgcaaca tccagtggca tcagtggtga tattccggcg atcccgtcat
    24181 cgctcattca tccaggcatt tattgctatg acatgttcta tcagaaagga aaaactcctt
    24241 ttctggcatg gtgtgagcag cgaggctcaa agcgtaatgc tgatggttta ggaatgctgg
    24301 tggcacaggc ggctcatgcc ttccttcttt ggcatggcgt tctgcctgat gtagaaccgg
    24361 tcatcaaact attgcaacag gaattgtcag cgtgaatcag gccatccagt ttccggatag
    24421 ggaagagtgg gacgagaata aaagatgtgt atgttttcct gctctcgtga atggtatgca
    24481 actgacatgt gcgatctctg gcgagagtct ggcgtatcgc tttactggag gtacgccaga
    24541 acagtggtta gcgagttttc gtcagcatcg ctgggacctg gaagaagaag cggaaaactt
    24601 aattcaggaa caaagtgaag atgatcaagg ttgggtctgg ttaccttgat ccagatattc
    24661 gtccttccat ttcacgtagt tatttgcgga atagcgtaac ccagccttct cttcatcact
    24721 taacgggcgg atctgtttga cgggactacc gagatacaga tatccgctct ccagccgttt
    24781 attttgtggg accaggctac ccgcaccaat catcacatca tcttctacta ttgcgccatc
    24841 aagtaaaatt gagcccatcc caaccaaaac tcgattgcca atggtgcagc cgtggagcat
    24901 caccttgtga ccaacagtga catcttcgcc aatggttaat gggttgccat ctgggttgta
    24961 cgaggattta tgagtgacat gcaacatact gccatcctgg atattggtgc gtgctccgat
    25021 ctgtacataa tgtacatctc cacgaatcac aacgagcggc cagatcccca catcatcagc
    25081 cagacgaacg tcaccaatca cgacgctgct atcgtcgatc attacgcgct ggccgatttg
    25141 tggaaaaaga tcgcggtatg ggcgtaaaac atcagacata ctcacctcaa caataaatga
    25201 tttcctaatg actttgggtg cattattagc cttgtgcaag tcttttagta tgcaaaaaag
    25261 caccgttttg tgtgcgaatg cagcaaaaag ggtgaaaaaa caacaaacag aaaaaaagat
    25321 caaaaaagta cttgtgcaaa aaattgggat ccctataatg cgcctccgtt gagacgacaa
    25381 cgtgaaacac ttcacaggat ggtcggaaca acgaagagaa aaaattctga aattcagggt
    25441 tgactctgaa agaggaaagc gtaatatacg ccacctcgcg acagtgagct gaaagccgcg
    25501 tcgc
//
LOCUS       DAZPJR010000048        25431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPJR010000048 DAZPJR010000000
VERSION     DAZPJR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.049"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            124..447
                     /gene="soxS"
                     /locus_tag="ACHVB7_001830"
     CDS             124..447
                     /gene="soxS"
                     /locus_tag="ACHVB7_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR1913836.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(450..2036)
                     /gene="pdeC"
                     /locus_tag="ACHVB7_001831"
     CDS             complement(450..2036)
                     /gene="pdeC"
                     /locus_tag="ACHVB7_001831"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR1913837.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2466..2746
                     /locus_tag="ACHVB7_001832"
                     /pseudo
     CDS             2466..2746
                     /locus_tag="ACHVB7_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2845..3381)
                     /gene="ssb1"
                     /locus_tag="ACHVB7_001833"
     CDS             complement(2845..3381)
                     /gene="ssb1"
                     /locus_tag="ACHVB7_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR1913838.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3635..6457
                     /gene="uvrA"
                     /locus_tag="ACHVB7_001834"
     CDS             3635..6457
                     /gene="uvrA"
                     /locus_tag="ACHVB7_001834"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR1913839.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6492..6848)
                     /locus_tag="ACHVB7_001835"
     CDS             complement(6492..6848)
                     /locus_tag="ACHVB7_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1913840.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6852..7268)
                     /locus_tag="ACHVB7_001836"
     CDS             complement(6852..7268)
                     /locus_tag="ACHVB7_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR1913841.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7379..8092)
                     /gene="aphA"
                     /locus_tag="ACHVB7_001837"
     CDS             complement(7379..8092)
                     /gene="aphA"
                     /locus_tag="ACHVB7_001837"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR1913842.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8494..8716
                     /locus_tag="ACHVB7_001838"
                     /pseudo
     CDS             8494..8716
                     /locus_tag="ACHVB7_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9220..10413)
                     /gene="tyrB"
                     /locus_tag="ACHVB7_001839"
     CDS             complement(9220..10413)
                     /gene="tyrB"
                     /locus_tag="ACHVB7_001839"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR1913843.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10666..11745)
                     /gene="alr"
                     /locus_tag="ACHVB7_001840"
     CDS             complement(10666..11745)
                     /gene="alr"
                     /locus_tag="ACHVB7_001840"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR1913844.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11798..13213)
                     /gene="dnaB"
                     /locus_tag="ACHVB7_001841"
     CDS             complement(11798..13213)
                     /gene="dnaB"
                     /locus_tag="ACHVB7_001841"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1913845.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13296..14279
                     /locus_tag="ACHVB7_001842"
     CDS             13296..14279
                     /locus_tag="ACHVB7_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR1913846.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14445..14687)
                     /gene="pspG"
                     /locus_tag="ACHVB7_001843"
     CDS             complement(14445..14687)
                     /gene="pspG"
                     /locus_tag="ACHVB7_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR1913847.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14821..15858)
                     /gene="dusA"
                     /locus_tag="ACHVB7_001844"
     CDS             complement(14821..15858)
                     /gene="dusA"
                     /locus_tag="ACHVB7_001844"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR1913848.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16221..16928)
                     /locus_tag="ACHVB7_001845"
     CDS             complement(16221..16928)
                     /locus_tag="ACHVB7_001845"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR1913849.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(16952..17206)
                     /gene="yjbL"
                     /locus_tag="ACHVB7_001846"
     CDS             complement(16952..17206)
                     /gene="yjbL"
                     /locus_tag="ACHVB7_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR1913850.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            17524..18039
                     /gene="zur"
                     /locus_tag="ACHVB7_001847"
     CDS             17524..18039
                     /gene="zur"
                     /locus_tag="ACHVB7_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR1913851.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18081..18290)
                     /locus_tag="ACHVB7_001848"
     CDS             complement(18081..18290)
                     /locus_tag="ACHVB7_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR1913852.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18406..19731)
                     /gene="dinF"
                     /locus_tag="ACHVB7_001849"
     CDS             complement(18406..19731)
                     /gene="dinF"
                     /locus_tag="ACHVB7_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR1913853.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19804..20412)
                     /gene="lexA"
                     /locus_tag="ACHVB7_001850"
     CDS             complement(19804..20412)
                     /gene="lexA"
                     /locus_tag="ACHVB7_001850"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR1913854.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20522..20890)
                     /gene="dgkA"
                     /locus_tag="ACHVB7_001851"
     CDS             complement(20522..20890)
                     /gene="dgkA"
                     /locus_tag="ACHVB7_001851"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR1913855.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21061..23484
                     /gene="plsB"
                     /locus_tag="ACHVB7_001852"
     CDS             21061..23484
                     /gene="plsB"
                     /locus_tag="ACHVB7_001852"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR1913856.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23639..24511)
                     /gene="ubiA"
                     /locus_tag="ACHVB7_001853"
     CDS             complement(23639..24511)
                     /gene="ubiA"
                     /locus_tag="ACHVB7_001853"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR1913857.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24524..25021)
                     /gene="ubiC"
                     /locus_tag="ACHVB7_001854"
     CDS             complement(24524..25021)
                     /gene="ubiC"
                     /locus_tag="ACHVB7_001854"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR1913858.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25244..>25431)
                     /locus_tag="ACHVB7_001855"
     CDS             complement(25244..>25431)
                     /locus_tag="ACHVB7_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913859.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
BASE COUNT         6578 a         6543 c         6090 g         6220 t
ORIGIN      
        1 gttagcagcg ctttaatgcg gggtaatttc ttttccataa atcgctttac ctcaagttaa
       61 cttgaggaat tatactcccc aacagatgaa ttaacgaact gaacactgaa aagaggcaga
      121 tttatgtccc atcagaaaat tattcaggat cttatcgcat ggattgacga gcatattgac
      181 cagccgctta acattgatgt agtcgcaaaa aaatcaggct attcaaagtg gtacttgcaa
      241 cgaatgttcc gcacggtgac gcatcagacg cttggcgatt acattcgcca acgtcgcctg
      301 ttactggccg ccgttgagtt gcgcaccacc gagcgtccga tttttgatat cgcaatggac
      361 ctgggttatg tctcgcagca gaccttctcc cgcgttttcc gtcggcagtt tgatcgcact
      421 cccagcgatt atcgccaccg cctgtagttt tattgcccgc gcgttaactc ccgcgcgggt
      481 aattgctcca tccattgcat aaacacctgc ggcggcatcg ccttcgcaaa aaaccatccc
      541 tggcaatagc gcacgccgcg tttgcgcagc cagttaacct gctcctcagt ttcgacgcct
      601 tcagcgatcg tttttaaccc caggctgtgc gccagctcga tgatgtgttc cgcaatcaaa
      661 tgactggttt tgtgggtggt cagcgtttca acaaacgatt tatcgatttt caaaatatcg
      721 acattcaatg atttaaggtt atgcaagtta gagtagccaa taccaaaatc atcaattgcc
      781 acttcgtaac cagcctggcg gaaagccaga ataatcggcg tcattttttc gacatcaaga
      841 aatgcatgct cagtcacttc aaatttaatt tgctgcggac gcaccgcgta ttgctctgtt
      901 ttctgattga ttcgcgctat caaccgtgac gtatgaaaat cggaggccga caggttaata
      961 gaaacatagc gatcggcatg tgttgccaag tacgcgccca gatcgcggaa gacattatca
     1021 ataacataat cagttatctg ttctatcatc ccctcctttt ctgccagcgg aataaactct
     1081 gccggattca ttatttgccc ctgctcacca agccaacgta acaacgcttc agcgccgata
     1141 catttttctg ttttgatatc gattattggc tggtaataaa gacaaagttg atgtttttcg
     1201 agggcgcgtt gcaatttacg tttgggagat aaatagtttt gtcgaatacg tagccagagt
     1261 aatagcagaa caagactccc caaaataccg gcgggcaacg caaaaataag atgattataa
     1321 aaatgggtta taagacgttg atatgaagtc gcaacaatgg ctgcaattgg gcgttttgtc
     1381 gaataaactg tcgcatataa atagccattt ctttgtacgg ttaaatcctt caaatgaatc
     1441 agcggagaaa acgttgctgc cgaggcctct ttgcttaacg agaaaaaggt tttcgtcacc
     1501 gtatcataca caccccattg caatgtcggg tcatcagaca tcacttcact ccagaagaga
     1561 gggttgataa ccgccacata atttccccgc tgcatatagg tcattttata gccagagaaa
     1621 aaaggcgtat cgcggtaata atagatagaa acgttaggtt cacgcttata atcggccggt
     1681 gcaatcgtat agccgtttac aggcgctatc agcgatgagc ataaaaaatg gttatcacgg
     1741 gcatagatca attcattaat ataaagatag ccacgaataa tattcaacat tcgcttttga
     1801 tgggctggag tgcaaacttg cccctgatag cgttcagctg catcgctcac taaatctgcc
     1861 tggcgaatga ccagctcgga tttatccagt gccagttgag caaaggttcg gagctgatta
     1921 ttcacttctg attttgccca aaggaaggca atccatagcg aaagaatgat gggaaagaga
     1981 actaaaaaga taatgcccgg caacgccagt aattggtgtc gtgcacgatg actcattgct
     2041 atcaatttcc tgcctgttta tacccggcca ttgcaaggtg aaaaccctgg gtaccgacgt
     2101 ctttagataa atcttacggt ataaatagca cttttgcctg atttgaaatt gatacctttc
     2161 taatcgtcac ctggatgaaa ataattcatt gatattcaaa attaatttcc tacaggaatg
     2221 cagaggcggc gggaagatat gcccttaatg ccactgaaaa accacataat ctgtacagca
     2281 ataaaagtca cggcctaata tggctaacaa acaatcaatt gtgatatagc tcacaaaatt
     2341 aatgaaacaa acagagtgtt tcatttttgt gttctgcaag ccccgcgata gccagagtgt
     2401 accacgcctc ccgtgaacaa cgccgcgctg tccagggcat cggctctttt cagaggaatt
     2461 gtttgatggc taccctcacc actggcgtgg ttcttcttcg ctggcagctt cttagtgccg
     2521 taatgatgtt tctggccagc acatcaacat ccgttttcgt cggtctgatt atgtcgggct
     2581 tgcggtgatc agcagcggtc tgggcgttgt ttccgcctgt tggttcgcaa tggggttgct
     2641 tggcatcaca atggcggaca tcaccgctat ctggcacaac atcgagtcgg tgatgataga
     2701 agagatgaat cagacaccgc cacaatggcc aatgattttg acctgatacg tagaagcctc
     2761 cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat
     2821 ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg
     2881 ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa
     2941 ctgattgcca ccctgcggct gctgaggctg cccccaaccg ccctgcggct gaccaccacc
     3001 gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc
     3061 gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt
     3121 acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc
     3181 cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc
     3241 gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc
     3301 accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt
     3361 gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta
     3421 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
     3481 aacaaacctg gccagacatt gttacacaac actccggata atgcattcca atactgtata
     3541 ttcattcagg tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
     3601 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
     3661 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
     3721 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
     3781 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     3841 aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     3901 gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg
     3961 tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc
     4021 gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat
     4081 gctgctcgcg ccaatcatta aagagcgcaa aggcgaacac accaaaacgc tggagaacct
     4141 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     4201 gccgaaactg gaactgcaaa agaaacacac tattgaagtg gtggttgatc gcttcaaggt
     4261 gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg
     4321 tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     4381 caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccgc gactgttttc
     4441 gtttaacaac ccggcaggtg cctgcccgac ctgtgacggc cttggcgtac agcaatattt
     4501 cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg
     4561 ctgggatcgc cgcaacttct attatttcca gatgctgaaa tcgctggcag atcactataa
     4621 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     4681 cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat
     4741 tcgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacgga
     4801 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg
     4861 cgaaggaacg cgtctgcgtc gggaagcgcg ccacgtgtat gtcgagaata cgccgctgcc
     4921 tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactcgc
     4981 aggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
     5041 cctcgttaac gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg
     5101 cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat
     5161 gtatgtactg gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg
     5221 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga
     5281 cgcaattcgc gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg
     5341 tgaagtggtc gcagaaggtc cgctggaagc gattatggcg gtgcctgaat cgttgaccgg
     5401 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc
     5461 agaaaaagtg ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac
     5521 gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
     5581 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
     5641 accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     5701 ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac
     5761 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     5821 acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat
     5881 caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa
     5941 acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct
     6001 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa
     6061 gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac
     6121 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     6181 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     6241 gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga
     6301 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     6361 cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
     6421 ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     6481 gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact
     6541 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg
     6601 agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc
     6661 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     6721 tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag
     6781 tcgttatgca cgctctgttc tgcgcctggt tttgccatgc aatattgtag caactccgaa
     6841 atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     6901 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac
     6961 ggcaatacaa gcgatgttcc cagcattgag gatttgatat gagaaggcat atcgtctgct
     7021 ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc
     7081 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     7141 tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga
     7201 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     7261 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     7321 ggataaaggt gtgattaaaa aaacagcatt cgggagagca tcacgctctc ccgctctgtc
     7381 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     7441 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg
     7501 taatatcgtt atcggaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg
     7561 attttgtatt ttgccctggt ttatcgcccg caaagattac cggattcatg ttggtggcag
     7621 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc gggctacgac
     7681 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     7741 cctcttttgg aatgctgaat tcatcccagc cattattcat tttttcccag aacacaggat
     7801 ttttcagata atcttcgctt tctggcgaga aggttttttt gccgcgccag aaccccggac
     7861 tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga
     7921 ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg
     7981 caacgttagt cccagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     8041 ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc
     8101 ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc
     8161 ataacgccag ctgccgggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga
     8221 tgattatatt taacccagta aatacgttcc tgtctttttt tgtataaaaa atgtttatta
     8281 ctcgatgtaa ggatacatta attttacgtt tattatgatg aatcctctgc aaatgagcga
     8341 aatttgaaag aacatttatc tgctgtacga tttaatgaac ttaatgatac agcattacaa
     8401 aaacaatcga agtttataaa gatgattcct gattgaccag aaccctatcc tacaatggtg
     8461 actaatttta ttactcccga tggagatgat gatatgaata tatcatatgt gaatagcaac
     8521 aaaacgacat ctttacctgt tgagctagat gcactaaata ataaagacat ctcttatgcc
     8581 aaagattttt cttatgctaa agatttcttt ttatacatag aaacacagtt aaaaattgca
     8641 aaagattttt gtagacctgg agaagaagta tcaagttcta ttgcaagtaa agtttttcac
     8701 gcatttatcg atttagttaa caaaatcagg ggtaagaaag actttatgta tatttgcacg
     8761 ctttgctgtt ttgccgagga ggttaaaggt gattattctc attacaggac ctttttattt
     8821 gatattggta atcaatataa ggttaaactt acacaaagcg gaaaaaaaga gttctcttta
     8881 acattagaat ttaacgatac tataattgaa tctcagaaag tcacaggcaa taaggcaaag
     8941 tatattcttg aggatataga aatattctat cgtaacaaac ccgatactta ttattaaatt
     9001 taataatggt tagatgtgca gaaattatta ctccacacga aaaaataggg aaaggaattc
     9061 cagataattg ggataaactt aacaataaaa gaactgacaa cgttttatta tctctcacgt
     9121 agaacaacgg catcaaaaaa atcatctaca aagaaatagt gaaagcgacg acagtttaat
     9181 ttcactgcag gctgggtaac tccagcctgc tttcctgcat tacatcaccg cagcaaacgc
     9241 ctttgccaca cgttgtacat ttgccgtatt taacccggcg acacacatgc gaccgctggc
     9301 gatgagatag acaccaaatt cttcacgtag tcggtcaacc tgagcggcac ttaaaccggt
     9361 ataactgaac atgccgcgct gattaagcag ataatcgaaa ttgcgttctg gcatctctgt
     9421 gcttaatacc ttcaccaatt cctgacgcat tgccagaatg cgagtacgca tctcttctac
     9481 ttccgccagc cagctggctt tcaatgcctc gtcattcagc actgcagcca ccacctgcgc
     9541 accaaaattc ggcgggctgg agtagttgcg gcgaactgtt gctttcaatt gccccagtac
     9601 gcggccagcg gcttcggcat cttcacacag aacagaaagt ccgccgacgc gctcgccgta
     9661 aagggagaaa attttcgaga acgaattgct caccagagcg ggtaatccag cgctggcaat
     9721 ggcgcgaata gcgtaggcat cctcttccat accggcacta aatccttgat aggcaatatc
     9781 gaggaatggg ataagctcgc gggctttgag aatttcaatc accgcatccc actgatcatt
     9841 agtgagatcg gcacccgttg ggttgtggca acatggatgc agcaacacaa tactgcgggc
     9901 aggtaatgtt ttcagcgtcg ccaacaggtc attaaagcgc acgctgttag tcgcttcgtc
     9961 ataccagggg taagtactca cttcgaatcc agccccggcg aatattgcta cgtggttttc
    10021 ccaggtagga tcgctgaccc agacgcctga ttccgggaag tagcgtttca ggaaatccgc
    10081 gcccactttc aatgcccctg agccgccaag ggtttgaatg gttgctacgc gctgttgttg
    10141 cagtaccgga tggtccgcac caaacagcag cggcgcaatg gcatggcgat agctgttaag
    10201 cccttccatc ggtaaataaa gcgaagcgcc atggggctgc gcattcaggc gcgcttccgc
    10261 ctccgccacg gctttcagtt gtggaataat tccgtcttcg ttgtagtaca gaccgatact
    10321 taaattcact ttgtcgctgc gagggtcttc tttaaaacgc tccataagcg taagaatcgg
    10381 gtcgccagcg taggcgtcaa ctttttgaaa cacgcgatgg ttctccaggt ttacgggcag
    10441 gtggttaaaa cacaataaac cggaagaagg cgaagatcga gtggatgttc aggagcgaac
    10501 ggcaattagc aacagagtga gactcatgac aaacgaacat ccgccagagg cacgaccttt
    10561 ataagagcat gaaagaacat caacttattg aatttttagg attttattgg ccggataagg
    10621 cattcacgcc gcatccggca cagacaatca aatattacag aacgattaat ccacgtattt
    10681 catcgcgacc cttgaagtca ggcgcgtaat aagttcgtaa gcgctcactt ttgtcatttc
    10741 agcgatacgt tctacgggca aaccttcgcc ccataaaatg accggatccc cggctttgtc
    10801 ctgcgcctgt ggacctaagt ctacgcagat catatccatc gccacgcgtc cgacaatcgg
    10861 tacttcgcga ccgttcacca gcactggcgt accggatgga gcggcgcgcg gataaccatc
    10921 gccatagccc atcgcgacta cgccaagacg ggtatcacgt tcgcttaccc aggttccacc
    10981 ataaccaaca ggctctccgg ctttatgctc acgcacggca atcaggctgg aggttagtga
    11041 catcactggc tgacagccaa aatcggcacc gatggagcga tcttccagcg gcgagacgcc
    11101 ataaagaatg atgcccgggc gcacccagtc aaaatgcgat tgtggccaca gcagaatgcc
    11161 acccgacgcg gcaatggaac gttgaccagg tttgccttcg caaaaggtat taaagatagc
    11221 gagttgtttc tcggttgcgc cacattttgg ttcatccgcg cgcgcaaaat ggctgacgat
    11281 attcaccggc tgacgaacgt ttttgcactg tgtcaggcga tgataaaacg cctcagcctg
    11341 ttccggcctt acgcccagac ggtgcatacc ggtatcgagt ttcatccaga cggtaaccgg
    11401 ctcgtccagg ctagcctctt ccagcgcagc cagctgttct tcgttatgca cggcggtatg
    11461 aaaatgttgc gcagaaatcg tcggcagatc tctggcatca aaaaagcctt cgagtaacag
    11521 tacaggtttg gtgattcccc ccgcacgcag tcgcagagct tcttcgagac gggctacgcc
    11581 aaaggcgtca gcatcgggga gcgttcgcgc ggtctcaaga agaccgtgac cataagcgtt
    11641 cgctttcacc accgcaacca ttttactggc aggggccagt tcacgaagac gttgcaggtt
    11701 gtgtcgcaga gcgcggcggt taatcacaac agttgccgct tgcatttgtg ttccttgata
    11761 agtgtttgct ttaattacct aattcataaa ataattatta ttcgtcgtcg tactgcggcc
    11821 ccgcatagtt gtcgaagcgc gaccattgac cgttaaaggt caggcgtacc gtcccgattg
    11881 ggccgttacg ttgtttaccg ataataattt ccgcgatgcc ttttaaatca ctgttttcgt
    11941 gatacacctc atcacgatag ataaacataa tcaagtccgc atcctgctcg atagagccag
    12001 attcacgcag gtcggagttg accgggcgtt tgtcggcacg ttgttccaga gaacggttca
    12061 actgggacag cgccaccacc ggcacgttca gttctttcgc cagtgctttc agcgagcgag
    12121 agatttctgc aatttccagc gtacggttat cggaaagcgc cggtacgcgc atcagttgca
    12181 ggtagtcgat catgataagc ccgatgccgc cgtgttcacg ggcaatacgg cgtgcgcggg
    12241 aacgcacttc cgttggcgtc aggccggagg agtcatcgat atagatattg cgtttttcga
    12301 gcaaaatacc catggtgccg gaaatgcgcg cccagtcttc atcatcgagc tgcccggtac
    12361 ggattttagt ctggtcaacg cgcgacagcg acgccagaga acgcatcata atctgttctg
    12421 aaggcatctc cagcgagaag ataagtactg gtttatcctg caacatcgcc gcgttttcga
    12481 cgaggttcat cgcaaatgtt gttttaccca tcgacggacg cgcggcgacg atgatcaaat
    12541 ccgacggctg caagccagcg gtttttttgt tgagatcgtc ataaccggtg tttactccgg
    12601 taacgccatc gtgtggctgc tgaaacaact gctcaatacg cgccacggtt gcgtcgagca
    12661 catcggcgat gttcttcggc ccttcgtctt tgtttgcacg actttcggca attttaaaga
    12721 cgcgggattc agcaaggtcc agcagatctt cgctggtacg cccctgcgga tcaaaaccgg
    12781 cttcagcaat ctcattcgca accgagatca tctcacggac aacggcacgt tcacgcacga
    12841 tgtcagcata agcactgatg ttcgccgcac ttggcgtatt ttttgacagc tctgccagat
    12901 aagcaaaacc accgacgcta tcgagttgcc cctggcgttc cagcgattcc gcaagggtaa
    12961 tcagatcgat aggactaccg ctttcctgca aacgcgccat ttcagtaaag atatggcggt
    13021 gtgggcgggt gtaaaaatcg tctgccacca cacgctcggc tacatcgtcc cagcgttcgt
    13081 tatccagcat taaaccgccc aacaccgact gctccgcttc gatcgagtgc ggaggcactt
    13141 tcagcccggc aacttgtgga tcgcgttcgc ggggttcagc ctgctgtttg ttgaagggtt
    13201 tatttcctgc catagtgaat ggagttaccg agataaagaa tgggtcgaaa ctttaccata
    13261 tgaagcagac cctgacgata cgttctggag gacacatggc aacacgaatt gaatttcaca
    13321 agcacggtgg cccggaagta cttcaagccg tagagttcac tcctgccgat ccggcagaga
    13381 atgaaatcca ggtcgaaaat aaagccatcg gcatcaattt tatcgacacg tatatccgca
    13441 gcggccttta cccgccgcca tcactaccca gcggattagg taccgaagcg gcaggcatcg
    13501 tgagtaaagt cggcagtggt gtaaagcata ttaaggcagg cgatcgtgta gtctatgcgc
    13561 agtcggcgtt aggcgcttac agctctgtgc ataacattaa tgcggataaa gcggcgattc
    13621 tgcctgcggc aatttctttt gagcaagctg cggcatcctt cctgaaaggc ttaacggttt
    13681 attatctgct gcgcaaaacc tatgaaatta aacccgatga acagttcctg ttccacgcag
    13741 cggctggcgg cgttggctta attgcctgcc agtgggcaaa agccctgggc gcgaaactta
    13801 tcggcaccgt aggaaccgcg caaaaagcgc agagcgcgct aaaagcgggc gcgtggcagg
    13861 ttattaacta tcgtgaagag gatctggtcg agcggttaaa agagatcacc ggcggtaaga
    13921 aagtgcgcgt ggtgtacgat tccgtgggca gagacacctg ggaacggtcg ctggattgcc
    13981 tgcaacgccg cggcttaatg gtcagttttg gcaactcatc aggtgcggtt accggtgtga
    14041 acttaggcat tctcaatcaa aaaggctcgt tgtatgtgac acgcccttcc ctgcaaggct
    14101 atatcaccac gcgggaggaa ttaaccgagg ccagtaatga actgttctct ttgattgcca
    14161 gcggtgtgat taaggtcgat gtcgccgagc agcagaaata tccgctgaag gatgcgcagc
    14221 gtgcgcatga gattctggaa agccgggcga cgcaaggttc cagcctgttg attccataaa
    14281 agaaataggg cttccacctg ggaagccctt tctttttata gttcggctgt atgtagggta
    14341 cagcacgatg aatctgttag aggcgcaata gtgacagatt tgattatcaa ttcctatttt
    14401 gttctaagga taaaacctta ggttgtgatc atccgcacaa tcccttagta acgccagcgg
    14461 tcataacgct gatatttcgg cacttttggt gctttaatcg ccttaataac ccacaccacc
    14521 gcaatcgcca gtagtaacca cggcagcaac ttaatcatca atgccagcat accgccgagg
    14581 aacataatgg ccgtcgccac aaccagcgcg gcgataatgc ccagcaacga aacgccggtg
    14641 accatcagca tgacaaaaaa gccaatcaca aaaagtagtt ccagcatgat gctctcccaa
    14701 atatgaaatc tcttgctggc attacaagaa tcatgccaaa aataatctat tgatttaaca
    14761 gcaaaacgcc ccgcgacggt gcgcagggcg tggtgaattt gactactttt tggtgaaaag
    14821 ttaacgctta tccgccacca gtttgagcgc gtgttccagc acattaatgt ctgcacccgc
    14881 tttatgggca ttttcactta aataacgccg ccactgccgc gcgccaggaa taccctggaa
    14941 caagcccaac atatgccggg taatatggcc gagatacgtc ccctggctga gttcacgctc
    15001 aatgtacgga tacatggcgc gcactaccgc caccggatcg gcatcggtat ccgaggaacc
    15061 aaagatctcc cggtctaccg ccgccagaat acccggattc tgatacgcct cgcgcccgac
    15121 catcacgcca tccatatgtt gcaggtgtgc tttggcctct tccagcgact tgataccacc
    15181 gttaatcgac attgtcagat gcggaaagtc acgcttcagt tgatacacac gcggataatc
    15241 gagcggcggg atttcacggt tttctttcgg gcttaacccc gaaagccagg ctttacgtgc
    15301 gtggatgata aacatctcac actcgccttt gccggaaacg gtgttgatga aatcgcagag
    15361 aaattcatag ctgtcctggt catcgatgcc aatacgcgtt ttcaccgtca ctggaatcga
    15421 caccacatcg cgcatcgctt tcacgcagtc ggcaaccagc tgcgcattac ccatcagaca
    15481 cgcaccaaac atgccgttct gcacccggtc agacgggcag ccgacattca ggttgatctc
    15541 atcatatcca cgcgcttctg ccagcttcgc acactgtgcc agcgccgccg gatcgctacc
    15601 gccgagttgc aacgctaccg gatgttcttc ttcactgtac gccaggtaat cacctttacc
    15661 gtgaataatc gcccctgtgg tcaccatttc ggtatacagc aacgtattgc gggaaagcag
    15721 acgcaagaaa tagcggcaat gtctgtccgt ccagtcgagc attggtgcaa cgctaaaccg
    15781 accactccag tgaacgtcag ttttttcagg cattgcgctg gtttggttga ttttttgcat
    15841 ttcagaatta ccgtgcattt tcaaatgtag agattatttt atcgatatat catggggtta
    15901 tgttattcag catcactatt caggaggctc aatagcgggg tactatacca taacaacagg
    15961 aagcgcctgt ctcattgcaa aagaaaattg agatcctctc aaggcatgaa gctctcacga
    16021 agtgatggaa ataatcttat tagccgttaa ctttgttaag gccaatgata aacaatccag
    16081 gttcgacgat aaataaaaaa tcacacatta aactctggtg atatatctcc ctgctaatag
    16141 cattgataga gaaaaaagaa cccaataaat attgggtcct tttatataat gccttccata
    16201 ctatcgaaga acttcacata ttatttctcc gatttaaccc cgaacaaatc ataaattaat
    16261 ttagaagtgt ctgtaagtat tttaatctct tcctttgagg ttgggtcaaa cgacttcgcg
    16321 aagctaatta attgtggtgc agcatctcgc attttgctta aaatatcagg atcgagcgtt
    16381 ccttcattca ccagatgtga catcttatcc agatagccat caaaattcat ttctccccca
    16441 tcagccagac gcttcatctc tcccaggtac ttcttcatat cacgttgaga taatttttca
    16501 aactgagctt tcaggtagct ctcattattt tcattaatat acactgtttc tgataaatct
    16561 gcgtaggcac tagaatatga aagtgaaatt aacagtgaag caaataattt taagctgttg
    16621 tcatcatgtt cctggcaggc attaacaaac gttaaaaaac cagaacctat tgcttgaaaa
    16681 tgtatattcg ctaaaggttc attatcctta gattcctcat aaaatagctg aaccgtggag
    16741 ggaaggccat tcttagtttc tacatcaaaa gtacattgtt caatgggagt cgcatcttta
    16801 ttatccccag gattacatat actattaatc gcgaaatgaa aaatagcccg gtcaacaact
    16861 gatgcaataa agtcacgatc gtttaaatcc tcatcagtgc aatcgtcaat atacttattg
    16921 acccacattt tgagatcatt tatttgctca attaaaaaca cattttttta cgtgcatgcc
    16981 ataaattatg cgtgattcgt tgaataaaca aatctatttt tgcaataatt atatcccgat
    17041 gacgcagcgc tgcataatca atagatttac catatatatt atttaattta cctttgattg
    17101 aacctctatt atctaattta tctttgattg catcttcatt atcgaattga tttgaattga
    17161 gtgttttatt aaagtatcca gttgcaccgg gtattatctt caacatgatt catctacctt
    17221 ttttgtcgta ccgtgtcctc cttataattc ttttgccatc tggtcactat caaaaatcgc
    17281 tcataatttc tgaactacag gtatgcgttt aaacccagtt aaataaatag cgagttaact
    17341 tgaagcaatt tatttaaaaa tgaattatta attattatcg tgttgataat ggtacgacaa
    17401 ggcaacataa caccttacct acaaaacaga aaatcaacat tacggcaaca ataagggttc
    17461 tcggtgtttt tcctggaaca tggtaaagta aggacattct taacccccac tttgaggtgc
    17521 ccgatggaaa agaccacaac gcaggagtta ttagcgcagg ctgaaaaaat ctgcgcgcag
    17581 cgtaatgtgc gcctgacccc acagcgcctg gaagtgttgc gcctgatgag tctgcaggat
    17641 ggcgctatca gcgcttatga tctgcttgat ttactgcgcg aagctgaacc gcaagccaag
    17701 ccgccaacgg tttatcgcgc gctggatttt ctgcttgagc aaggttttgt gcataaggtg
    17761 gaatccacca acagttatgt gctctgtcat ctgttcgacc agcccacgca tacgtcagcc
    17821 atgtttatat gcgatcgctg cggcgcggtg aaagaagagt gtgcagaagg cgtggaagac
    17881 attatgcata cgctggcggc aaaaatgggg tttgccctgc ggcataatgt gattgaagca
    17941 catgggctct gtgcggcatg cgtggaagtg gaagcgtgtc gtcatcctga acagtgccag
    18001 catgatcact ctgtgcaggt gaaaaagaaa ccgcgttaag agggcgtaca tccttgtaca
    18061 cgtcgggcag gagggattaa ttaccagcga tattcattgc gggtttccca atccacgacc
    18121 tctttttctg cctgatcttt ctgataacca taccgttcct ggattttacc gaccagttga
    18181 tcacgtttac cttcaatgat cgtcatatca tcatcggtca gtttgcccca ttgctctttc
    18241 actttacctt taaactgttt ccagttaccg ccggcttcat ctttattcat aatcaagacc
    18301 tcatcgttag gttgtgaatg agagtacgtt cacttttctt ctgaacgtga gattaagtgt
    18361 agtcagcaat ttggcttagg ataatttatt cagaattttt aaccgtcacg ttgcggcaaa
    18421 ccaggtaccg ttacgccagt gacgccgcca gatagccgcc agagaaagcc cgcgcaacgc
    18481 cagaaagacg gttagtgcca gccacaaacc atgattaccc agccacggca gcgtaaggag
    18541 cgtcagcgca aaacctgcgg cggccaccgc catactgtta cgcatttcgg cggcacgcgt
    18601 tgcgcctata aacatgccgt ccagcagata acaccagacg ccaaccaacg gcaaaatcac
    18661 ctgccagata agatagcggt cagccagctg ctgaatctgg gttaacgacg tcagcaacgc
    18721 aatgatgtgt tcccctgcca gcaaataaac caccgaaaat agtaacgcta cgatccccga
    18781 ctggcggcac gctgcccgcc agacatccag caactggcta ccgtcgcgcg caccgtatgc
    18841 ctgaccggaa tgtgcttcaa ccgcgtaggc aaaaccatcc agcgcatagg cggtaaaggt
    18901 gagtagcgtc atcagaaccg cgtttacagc gataatgtca ctccccagtc gcgcgccaag
    18961 tacggtgatc gcgccgaaac agagttgcaa caacagcgaa cgcagcatga tatcgcggtt
    19021 aagcgccagc aagcgacgga agtttcctcg ccaggcagtt ttcgacattt cgccggaaat
    19081 tccgcgtagt ttgaggattt tacgcaccat tagcagacca atcagcaatg ttgcatattc
    19141 cgcaataacc gtcgccagag ccgcgccctg cacgttcata tgcagcccca tcactagcca
    19201 gacatccagc acaatgttga ggatattgcc gaccactaac aaaattactg gcgcacgggc
    19261 atattgcacg ccgagtaacc aaccgagtaa taccagattc gccagcgacg ccggtgcgct
    19321 taaccagcgg atttcaagaa agcgccgcgc ctgttccagg actgcttcac taccgccaac
    19381 aatatgcaga gccagatcga taatcggtgt acgcagcagc gcaattaacg ccccagcccc
    19441 caacgccaac agcaacggtt gcaccagcgc acgggctaat gcctgaggat ttttggcacc
    19501 ataagcctgc gcagtcagcc cggtggtgct catgcgtaaa aacagcaaca gcataaagag
    19561 aaagctggtc gccgttgcac caaccgccac gccgcccaaa taaaccgggc tatcaagatg
    19621 accaattacc gccgtatcga ccagtcccag caacggaacg gtgatattgg agaaaatcat
    19681 gggtaaggcg agatgccaga gtgctttatc agatgaagtg aggaatgcca tgcagacaag
    19741 cctgatgaag agagatgaaa aacaaaccgc gacgcctggc ggcatcgcgg tctcagagat
    19801 atgttacagc cagtcgccgt tgcgaataac cccaaccgcc agcccttcaa tggtgaagct
    19861 ctgctgacga aggtcaacga caattggttt aaactcgcta ttttctggca acagttcgac
    19921 tttattaccc tgttttttca ggcgcttaac ggtaacttcg tcatcaatac gtgcgacaac
    19981 gacctgaccg ttacgtacat cctgagtttt atgcactgcc agcaagtcac catccataat
    20041 gccgatatct ttcatcgaca tcccgctgac gcgcagcagg aaatcagcat tcggcttgaa
    20101 caaggaagga tcgacctgat aatgaccttc aatatgctgc tgcgccagaa gcggttcacc
    20161 ggcagccaca cgacctacca gcggcaaccc ttcttcctct tcctgcaaca gacgaatccc
    20221 gcgtgatgcg ccggaaacaa tttcaataac gcctttgcgt gccagcgcct tcagatgttc
    20281 ttcagccgcg tttggggaac ggaaccccaa acgctgcgcg atttccgcac gcgtcggcgg
    20341 catacctgtc tggctgatgt gatcacggat gagatcaaac acctcttgtt gcctggccgt
    20401 taacgctttc attccgcccc ctgggtgtat atacagttat gctgtgagta tatacagcaa
    20461 aaggcgattt tggaaccata aactgcacaa taaaccagag atttatcgaa atctggaagg
    20521 gttatccaaa atgcgaccat aacaggatgc accaggttat cacggcgacg ataatcgcaa
    20581 tcagcaccgc ggcggatccc atatctttag cgcgtccgga aagctcatgg tattcagagc
    20641 caattcggtc aaccactgct tcgatggcgc tattgagaat ttccacaatc atcaccagca
    20701 tcacggagct gataagcagc acgcgggtaa ttgcgtccac atccagccag caggcgatga
    20761 ccaccgccaa caataccgct acgccttcct gacggaatgc cgcttcgttg atccatgcag
    20821 cgcgtaaacc tttccaggaa tagccagcag ctttgataat tcgggtgaat ccagtggtat
    20881 tattggccat tgaaagaacc tttttacatt atgagcgtca atatcagtgt accgttaaat
    20941 ccgatgattc gggtttgcgt aacgccgcgc gaaacatgag cggataccac agaatttccc
    21001 atgactttct gctatccttg ccgcgcattt gcattattaa ccagaggctt tacatcgttt
    21061 atgtccggct ggccacgaat ttactacaaa ttactgaatt taccattaag catcctggta
    21121 aaaagcaagt ctattccggc agatcctgcc ccggaactgg ggctggatac ctctcgtcca
    21181 attatgtacg ttttaccgta caactcgaaa gcagatttgc tgacgttacg cgcccagtgt
    21241 ctggctcatg acttgcctga cccgttagag ccgctagaaa tcgacggcac gctactgccg
    21301 cgctatgtgt tcattcacgg cgggccgcgt gtattcacct attacacgcc gaaagaagag
    21361 tctattaagc tgttccacga ctatctcgat ttgcaccgta gcaacccaaa tctggatgtg
    21421 cagatggtgc cagtatcggt gatgtttggt cgcgcgccgg ggcgtgaaaa aggcgaagta
    21481 aacccgccgc tgcgtatgct taacggcgta cagaaatttt tcgccgtact gtggctcggt
    21541 cgcgacagtt ttgtgcgttt ctcgccgtca gtttcgctgc gccgtatggc ggatgaacac
    21601 ggcacggata aaactatcgc acagaaactg gcgcgcgtgg cgcgtatgca ctttgcccgt
    21661 caacgtctgg ctgccgtagg cccacgtctg cctgctcgtc aggatctgtt taataagctg
    21721 ctcgcctccc gcgccattgc caaagcggta gaagatgaag cgcgcagcaa aaaaatctcc
    21781 catgaaaaag cgcagcagaa cgcgattgcg ctgatggaag agattgcggc gaatttctct
    21841 tacgagatga tccgcctgac tgaccgcatt ctgggcttca cctggaaccg actttatcag
    21901 ggcatcaacg tccataacgc cgagcgcgtt cgccagctgg cccacgacgg tcatgagctg
    21961 gtgtatgtgc cttgccaccg cagtcacatg gactacctgc tactttctta cgtgctgtac
    22021 caccaggggc tggtgccgcc gcatatcgcc gccgggatca acctgaactt ctggccggcc
    22081 gggccgattt tccgccgtct gggggcgttc tttattcgcc gtacttttaa aggcaataaa
    22141 ctttattcca ccgttttccg tgagtatctc ggcgaactgt tcagccgtgg ttattccgtc
    22201 gagtacttcg tggaaggcgg tcgttcccgc acggggcgtt tgctggatcc gaaaaccggt
    22261 acgctgtcga tgaccattca ggcgatgctg cgtggcggca cgcgtccgat tacgctgatt
    22321 ccgatctata tcggttatga gcacgtcatg gaagtgggta cttacgccaa agaactgcgc
    22381 ggcgcgacga aagagaaaga gagcctgccg cagatgctgc gcggtttaag caagctgcgt
    22441 aatctcggtc agggttacgt caacttcggt gaaccaatgc cgttgatgac ctaccttaac
    22501 cagcacgtac cagactggcg tgaatctatc gatcccatcg aagcggtgcg tccggcctgg
    22561 ttaacgccga cggtcaataa tattgctgcc gatctgatgg tacgcattaa caacgcaggc
    22621 gcggcaaacg ccatgaacct gtgttgtact gcgcttctgg catcacgtca gcgctcactc
    22681 acccgcgagc agttaaccga gcaactcaac tgctacctgg atctgatgcg caacgtacct
    22741 tactccacgg actctaccgt tccttcagcc agcgccagcg agcttatcga tcacgcgctg
    22801 caaatgaaca agtttgaagt cgagaaagac actatcggcg acatcatcat tctgccgcgc
    22861 gagcaagcgg tgctgatgac ctactatcgc aacaacattg cgcatatgtt ggtgctgcct
    22921 tcgctgatgg cggcaattgt cacccagcat cgccacatct cccgcgacgt attgatggag
    22981 cacgtcaatg tgctttaccc aatgctgaaa gcggagctgt tcctgcgctg ggatcgcgac
    23041 gagttgccgg acgttattga tgcgctggca aatgagatgc aacgtcaggg gctgattacc
    23101 ctgcaagatg atgagttgca tatcaacccg gcgcattctc gccccctaca gctgctggcc
    23161 gcaggcgcgc gcgaaacgct gcaacgttat gccatcacct tctggttgtt gagtgccaac
    23221 ccgtcgatca accgcggtac gctggagaaa gagagccgca ccgttgcgca acgtctctcc
    23281 gtgctgcacg gcatcaacgc gccggagttc ttcgacaagg cggtgttcag ttctctggtg
    23341 ctgacgctgc gtgatgaagg gtatatcagc gatagcggcg acgccgaacc ggcagaaacg
    23401 atgaaggttt atcagttgct ggcggagttg attacatcag acgtgcgttt gacgattgag
    23461 agtgcgacgc agggcgaagg gtaatcagag agaattgccg gatgcggcga aaacgcctta
    23521 tccggccttc cataatctgc aaattcaata aattgcgatg tgttgtgtag gcctgagaag
    23581 cgcagcgcat caggcaaccc agaagaaagc cggatgatca tccggctttt ttacatcatc
    23641 agaaatgcca gtaactcatt gccagtccta aaaacagtac cagaccaaca tagttattat
    23701 tcataaatgc tttaaagcag gcttcacgct cgcggttggc aatcagtttt tgttgataaa
    23761 caaacagcgc gccagccacc agaattgacc agtaatatcc ccagcctaag ccatttaact
    23821 caccgatgat cgccatcagt gccagtacgc caatctgcaa aataccaata atcaatttat
    23881 cgtattggcc gaacaggatt gcggtggatt taataccaat cttcacatca tcgtcacggt
    23941 caaccattgc atactgcgtg tcgtaagcca ccgcccagag aatattggcg aggaacatta
    24001 accagcaact caatggcacc gactcactca cagcggcaaa agccattgga atcgaccagc
    24061 caaacgccgc gcccagcacc acttgcggga gatgggtata ccgcttcata aacgggtaca
    24121 cccacgccaa cgccagagcg gcaatcgaca acagaatggt catcgtattc agcgtcagca
    24181 ccagtaaaaa tgaaatcaat accagcacta caaacagcgc gcgcgcctct ttctccgtta
    24241 ccgcgccgct gggaagcggt cggttcgccg tgcgcttaac atgaccatca aacttgcggt
    24301 cagcataatc attcaccaca catccggcag cgcgcatcag ccagacaccc gcgacaaaca
    24361 ccgccaggat ccagagctgg ggaacgcccg gtgtcgccac ccacaacgcc cataatgttg
    24421 gccagagcag cagtaacgcg ccaattggct tatccgtacg cattaagcga tgaaacgcca
    24481 gcagcttatt ctgcgtcaga ctccactcca tatttttttc ctcttagtac aacggtgacg
    24541 ccggtaaaaa cagttctgtt agcaacagcg gtttaccgct taatcgcagg cgggaacgtc
    24601 gcccccacag tccggcatca cggcctatct caataaagtc ccgggttaat gtcgatgatg
    24661 tgaacagata gcgtcctagc ggcgttttac ccaatttttg taacgccagc tccggcccgc
    24721 ttaacgttga cacaggaacg acggtacgac cggcaagcca cggttcacca tcggcacata
    24781 acaaaatttc acgtaaccag taacgagact ctttcggcag cagcggcagt tcttcgggga
    24841 tttcattctg ctcgacaaac ccttcgcgga tcatcgtcac gctcaccgtt tttccctgct
    24901 gttcaaaacg ttttgtcatg gaatcttcca gcaacagcca gtcgagcagt tgcgaatcca
    24961 gggcagggat ctctttaaaa tagcgcagcg cacgcagttg cgttaacgcg gggtgtgaca
    25021 tgccgaactc tccgttacat aacgtaaagg cattgtatcg cagaaaagtg tttacgggag
    25081 atgtaaagca aattaagtga tctaatgcgc aacaaaaacg caacaatcgc atgaagtggt
    25141 aaaacgctgt tgaaccttac gctttgccga tgcccgtgct ggcgagggca accgtagggg
    25201 catcaccgcc catgatgcca ggaacggtga tgttttcctt tacttaaata cctatattct
    25261 ttatacgagt agctcgccaa ctcgcagtaa aaattgggaa aagcgcatat ttaccatgag
    25321 atttggagct aaaaagtgtt tcctgagtaa gattaatagg caacatctta tgtctggatt
    25381 tatagataca cgtggaattc caattggtat cttttgtagg attcaacata t
//
LOCUS       DAZPJR010000049        25104 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPJR010000049 DAZPJR010000000
VERSION     DAZPJR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.050"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            194..382
                     /gene="bcsR"
                     /locus_tag="ACHVB7_001856"
     CDS             194..382
                     /gene="bcsR"
                     /locus_tag="ACHVB7_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR1913860.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="ACHVB7_001857"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="ACHVB7_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR1913861.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="ACHVB7_001858"
     CDS             1143..1307
                     /locus_tag="ACHVB7_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR1913862.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1314..3761
                     /gene="bcsA"
                     /locus_tag="ACHVB7_001859"
                     /pseudo
     CDS             1314..3761
                     /gene="bcsA"
                     /locus_tag="ACHVB7_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="ACHVB7_001860"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="ACHVB7_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR1913863.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="ACHVB7_001861"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="ACHVB7_001861"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR1913864.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="ACHVB7_001862"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="ACHVB7_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="ACHVB7_001863"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="ACHVB7_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR1913865.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /gene="dctA"
                     /locus_tag="ACHVB7_001864"
     CDS             12951..14237
                     /gene="dctA"
                     /locus_tag="ACHVB7_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR1913866.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="ACHVB7_001865"
     CDS             14457..15953
                     /locus_tag="ACHVB7_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1913867.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="ACHVB7_001866"
     CDS             complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="ACHVB7_001866"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR1913868.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="ACHVB7_001867"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="ACHVB7_001867"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR1913869.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="ACHVB7_001868"
     CDS             18047..20107
                     /locus_tag="ACHVB7_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1913870.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="ACHVB7_001869"
     CDS             complement(20289..21611)
                     /locus_tag="ACHVB7_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1913871.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="ACHVB7_001870"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="ACHVB7_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR1913872.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..23983)
                     /locus_tag="ACHVB7_001871"
     CDS             complement(23084..23983)
                     /locus_tag="ACHVB7_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1913873.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..>25104
                     /locus_tag="ACHVB7_001872"
     CDS             24503..>25104
                     /locus_tag="ACHVB7_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR1913874.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6991 g         5854 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctg
//
LOCUS       DAZPJR010000050        24734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPJR010000050 DAZPJR010000000
VERSION     DAZPJR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.051"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(84..3530)
                     /gene="mfd"
                     /locus_tag="ACHVB7_001873"
     CDS             complement(84..3530)
                     /gene="mfd"
                     /locus_tag="ACHVB7_001873"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR1913875.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(3658..4731)
                     /locus_tag="ACHVB7_001874"
     CDS             complement(3658..4731)
                     /locus_tag="ACHVB7_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1913876.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            4993..6192
                     /gene="lolC"
                     /locus_tag="ACHVB7_001875"
     CDS             4993..6192
                     /gene="lolC"
                     /locus_tag="ACHVB7_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR1913877.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            6185..6886
                     /gene="lolD"
                     /locus_tag="ACHVB7_001876"
     CDS             6185..6886
                     /gene="lolD"
                     /locus_tag="ACHVB7_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR1913878.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            6886..8130
                     /gene="lolE"
                     /locus_tag="ACHVB7_001877"
     CDS             6886..8130
                     /gene="lolE"
                     /locus_tag="ACHVB7_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR1913879.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            8159..9070
                     /gene="nagK"
                     /locus_tag="ACHVB7_001878"
     CDS             8159..9070
                     /gene="nagK"
                     /locus_tag="ACHVB7_001878"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR1913880.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            9086..9907
                     /gene="cobB"
                     /locus_tag="ACHVB7_001879"
     CDS             9086..9907
                     /gene="cobB"
                     /locus_tag="ACHVB7_001879"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR1913881.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(10063..11109)
                     /gene="potD"
                     /locus_tag="ACHVB7_001880"
     CDS             complement(10063..11109)
                     /gene="potD"
                     /locus_tag="ACHVB7_001880"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR1913882.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(11106..11900)
                     /gene="potC"
                     /locus_tag="ACHVB7_001881"
     CDS             complement(11106..11900)
                     /gene="potC"
                     /locus_tag="ACHVB7_001881"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR1913883.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(11897..12754)
                     /gene="potB"
                     /locus_tag="ACHVB7_001882"
     CDS             complement(11897..12754)
                     /gene="potB"
                     /locus_tag="ACHVB7_001882"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR1913884.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(12738..13874)
                     /gene="potA"
                     /locus_tag="ACHVB7_001883"
     CDS             complement(12738..13874)
                     /gene="potA"
                     /locus_tag="ACHVB7_001883"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR1913885.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            14124..15350
                     /gene="pepT"
                     /locus_tag="ACHVB7_001884"
     CDS             14124..15350
                     /gene="pepT"
                     /locus_tag="ACHVB7_001884"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR1913886.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(15399..16520)
                     /gene="roxA"
                     /locus_tag="ACHVB7_001885"
                     /gene_synonym="ycfD"
     CDS             complement(15399..16520)
                     /gene="roxA"
                     /locus_tag="ACHVB7_001885"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR1913887.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(16596..18056)
                     /gene="phoQ"
                     /locus_tag="ACHVB7_001886"
     CDS             complement(16596..18056)
                     /gene="phoQ"
                     /locus_tag="ACHVB7_001886"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR1913888.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(18056..18727)
                     /gene="phoP"
                     /locus_tag="ACHVB7_001887"
     CDS             complement(18056..18727)
                     /gene="phoP"
                     /locus_tag="ACHVB7_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR1913889.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(18895..20265)
                     /gene="purB"
                     /locus_tag="ACHVB7_001888"
     CDS             complement(18895..20265)
                     /gene="purB"
                     /locus_tag="ACHVB7_001888"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR1913890.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(20269..20910)
                     /gene="hflD"
                     /locus_tag="ACHVB7_001889"
     CDS             complement(20269..20910)
                     /gene="hflD"
                     /locus_tag="ACHVB7_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR1913891.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(20946..22052)
                     /gene="mnmA"
                     /locus_tag="ACHVB7_001890"
                     /gene_synonym="trmU"
     CDS             complement(20946..22052)
                     /gene="mnmA"
                     /locus_tag="ACHVB7_001890"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR1913892.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(22106..22567)
                     /gene="nudJ"
                     /locus_tag="ACHVB7_001891"
     CDS             complement(22106..22567)
                     /gene="nudJ"
                     /locus_tag="ACHVB7_001891"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR1913893.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(22577..23230)
                     /gene="rluE"
                     /locus_tag="ACHVB7_001892"
     CDS             complement(22577..23230)
                     /gene="rluE"
                     /locus_tag="ACHVB7_001892"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR1913894.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            23402..24652
                     /gene="icd"
                     /locus_tag="ACHVB7_001893"
     CDS             23402..24652
                     /gene="icd"
                     /locus_tag="ACHVB7_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR1913895.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
BASE COUNT         5984 a         6304 c         6463 g         5983 t
ORIGIN      
        1 gtgacagtgt cggatagtgc agggaagtgc aaagaatttg taaatgttgc agatgggggc
       61 gcagaaacgc ccccgattta ccattaagcg atcgcgttct cttccagttc acgcataaac
      121 tggcgtaccc attcgatacg cgttttccgc tcactcaaat cctgaataaa tttcaggcgc
      181 gtcggaccat caaggcggta atgctgcggc tgtttttgca gcaaaccaat caaccaggcc
      241 ggattaacgt gattcttctc ggcaaattcg atcaccccgc ctttctcatt accttccagc
      301 ttcctgatcc ccagtttctg cgcttgctgg cgcagacggg caatatccag cagggtacgc
      361 gccggatctg gcagcaggcc gaagcgatcg ataagctcga ctttgatctc ttccagttcg
      421 ttttccgttt tggcgctggc aatacgttta tagaacgaca aacgcgtatt cacgtcagga
      481 atgaaatcat ctggcaatag cgacggcatc cgcagctcga cttctgtttg ctggctggtg
      541 agatcttcca gcgacggctc gcgtccggct ttcagtgcat cgacggcgtt ttccagcaac
      601 tccatataca gcgagaaacc gatggtttcc attgagccgc tttgttcttc gccaagcagt
      661 tcacccgcgc cgcgaatctc cagatcgtgc gttgccagcg caaaacctgc cccgagatct
      721 tccagcgagg caatcgcttc aagacgtttt tgtgcatcgg tagtcatcgc ttttggatgc
      781 ggtgtcagca accatgcata tgcctgatga tgcgaacgtc cgacgcgacc gcgtaactgg
      841 tgcagctgcg ccagaccgaa gtgatccgcg cgttcaatga taatggtgtt ggctgtcggg
      901 atgtcgatcc cggtttcgat aatggttgta caaaccagca cgttgaaacg ttgatgatgg
      961 aaatcattca tcacccgttc cagttcgcgc tcgcgcatct gcccgtgacc gatggcgatc
     1021 cgcgcttctg gcaccagttc tgccagccgt tcggcagctt tctggatgtt ttccacatca
     1081 ttgtagagat aataaacctg ccccccgcgc aaaatttcac gcaggatcgc ctcccggacc
     1141 actaggctgt catactcacg gacaaaggtt ttaactgcca gacgacgggc gggcggcgtg
     1201 gcgataatcg acaggtcacg cattccgctc attgccatat tcagcgtgcg tgggatcggc
     1261 gttgcagtaa gcgtcagaat atccacgttc gcgcgcatcg ctttaatgcg ctctttatga
     1321 cgcaccccga agcggtgttc ttcatcgaca atcagcaggc ctaaatcttt aaacttgacg
     1381 tcactttgca gcagtttgtg cgtaccgatc agaatatcga ttttcccttc cgccacttcc
     1441 gcaaggattt gcgtctgctc tttggcgctg cggaaacggg agatcatttc gatacgtacc
     1501 ggccagttgg cgaaacggtc gcggaagttg tcgtaatgct gctgcgcgag aagggtggta
     1561 ggcaccaaca ccgccacctg cttgtggtta tctactgcca ggaaagcggc gcgcatcgcc
     1621 acttctgttt taccaaagcc aacatcgccg cacaccagac gatccattgc cagcggctga
     1681 cacatgtcgc taagtaccgc attaatggcc tgcgcctgat ccggcgtggt ttcaaacggg
     1741 aagctgtcgc agaacaactg atactgctca cgatcgtgtt taaacgcgaa gccctctttg
     1801 gcggcgcgtt gcgcgtagat atccagcaat tccgccgcca catcacgcac tttttccgcc
     1861 gctttctgcc gcgcgcgtga ccacgcatcg ccgccaagtt tatgcagcgg ggcgttttct
     1921 tccgcgccac ctgcgtaacg gctaatcaga tgcagtgacg acaccggaac atacagtttg
     1981 gcgtcgttgg cataggtgag catcaaatac tcgccagtaa tgccgcccgc ttcgagcgtg
     2041 gtcattccgg catagcgccc gacaccgtgc tccagatgga ccaccggctg accaatatgc
     2101 agctccgcaa ggttacggat cagtgtatcg gggttgatgg tgcggcgaga atcctgacga
     2161 cggcgggcaa cacgttcacc gagcagatcg ctttcgcaga tcagcgccag attacgcacc
     2221 ttatcgacaa aaccatgttc ggcagcgcca atcatcagat aacgcccacg gtcgctggct
     2281 tcatcaagac gcataatgcg ttgcggagca attttaattc gtgcgagcag ttcacccagc
     2341 gcttcacggc gaccttcact ttctaccgag aacaccaccg gaccgtcgaa agtctcgagg
     2401 aacttacgca gcgcatccag cggcgctttt tgttgtgcct gaacggccag gtctggcagt
     2461 ttctggaaac ctaaattggc attcgcggct tttgtcggta aatgttcagt ttttagctgc
     2521 acccggggcc agtttttcag ctctgagaag agctcgtcca cccgcagcca gagcgattgt
     2581 ggtggcaaca gcgggcgcat cggatcgacg ccgcgattct caaaacgcgc cagcgtgtca
     2641 gcctggaaac gttcggcact ggtttccaga tcgccagtat tcacgagcaa ggtattggca
     2701 gggaaataac tgaacagcgg cggcagtggt tcgctgaaga acaatggctg ccagtactcg
     2761 atcccggcag gtaatgtgcc tttactcact tgctggtaaa tatgttccgg atcgcgcttc
     2821 acttcgaagg tatcgcgcca ctggctgcgg aacagttcaa ttgccgcttt atcggtcgga
     2881 aattcgtgcg cgggcagcag attgatcgct tctacttcct ccagcgtgcg ctggctgtcg
     2941 acgtcaaaca cccgcaggct gtcgatttca tcatcaaaga aatcaagacg ataaggcagc
     3001 tcactcccca tcgggaagag atccagcaac gcgccgcgcg tggcgtattc gccgtgctcc
     3061 atcacctggt caacatggcg ataaccggcg ctgtccagtt gggttcgtaa tgcatctcgt
     3121 gacaggcgct gacctttttt catcaccagc gcatgaccgt ggagaaaact gtgtgggcaa
     3181 acgcgctgca taagcgtatt caccggaaca atcagtacgc cacgctgcat cgtcggtagc
     3241 tggtaaaggg tggaaaggcg cgaggagata atgtcctgat gaggcgaaaa actgtcgtag
     3301 ggaagagttt cccagtccgc cagattcatc accatctgat cggtgaactg gctgatttca
     3361 tcatgcaaac gcagagcatt ttgcatatct ggtgcaatga gtaccaccgg accggcgtga
     3421 cgttcggcaa tttccgctac cagcgttgca caggctgcgc cggttaactc gcccagcaga
     3481 cgctgctcac ccgctttgac gggcagtgta taacgatatt gttcaggcat aacggttgtc
     3541 agattctcgt taggatatgc ctcaacatat gggggcatat ctctgataag caatgctgtt
     3601 cattatccgt aatcgacatg gtttagcaaa ttgaatcgcc ccggctgggg cgatggttta
     3661 acgagagtaa ctgacggcag cgggcagtga aggcggggag aacaacagat cgctgaatgc
     3721 acgctgtgaa agttttctta ccagcaagcc cgcaacagta cagatgaaca aactgacaaa
     3781 gggataaacc agcaggagtg tcagttcgac ttgcggcgac cagcgtgctg cgttcatttg
     3841 cgcaagcaga gttaagctga atatttcaac cagaatgcga tgggtggtgt agatagcaat
     3901 ggtgttggag ccaatcacat tcagcaggct ggtggagcgc ataccgaatc gttgctcgta
     3961 ctgataaaac agcttcatga tcaccacaat cgataccagc gacaacagca gcgagatagt
     4021 aaacaaccag gcaccgaccg ccagaacggt aagcaaagaa gccatcagca gatggcggcg
     4081 caacggcacc tctttaacac aggtcattat tgttgcgcca aaccatgcgc caaggctgta
     4141 ataaggcaaa ttgcggatca cactgttcat tccccaccac ggcgtgggaa cgaaattaac
     4201 cgccacactc agcagtacaa acaaggcgaa tagtggcagc gccagacggc taaaaatttt
     4261 acataccacg aaatagacaa ttaacgcata cagataccac aagctggtgc tggcggtgat
     4321 catcccgtgc aggaactcac cggtggaatc ggcataagcg gcattggagg cattgcttaa
     4381 atcgcgctca ggtgccagcc actggttcag cgcacttaac gccagccact gcaccacgcc
     4441 ccaaagcgcc agcacccaga agatgttcca gatccgttta tcgagacaat ttccccacgg
     4501 tacgctgtcg atatagcggc gaatcaaata gccggatata aagaaaaaaa ccggcatacg
     4561 aaagggtgca aggtaaagat tgaaatagat ccagcattta ctcaggactt ccgataacgg
     4621 atgctgaaaa gtggtcatat gcggataaaa ggtaatgacc gagtgataaa tcaccaccag
     4681 acaaatacat aaccctttga tctggttaat ccatagctct ttttgtttca tcaggacaac
     4741 acgctctttt ttaatttaca tgcggttgat cctgcgggtt atgacccgag gtgtaatggg
     4801 tatctgtctg tattggatga tttttcagat taagatcagg cggcaagatt gatgataaaa
     4861 catggcaatt tagccgattg atttacgggg gcttttcaga ttagccctga cgatcactta
     4921 cagttcagac gtttacccat cttgctttcg cttatatact cgtgtctttg ctacagcaac
     4981 cagacggatt tcatgtacca acctgtcgct ctatttattg gcctgcgtta catgcgtggg
     5041 cgtgcagcgg atcgcttcgg tcgtttcgtc tcctggcttt ctaccatcgg cattaccctc
     5101 ggggtgatgg cgctggtcac agtattgtca gtgatgaacg gctttgagcg cgagctgcaa
     5161 aacaacatcc ttggcctgat gccacaggca attctctctt ctgagcatgg ctctcttaac
     5221 ccgcagcaac tcccggaaac ggcagtcaaa ctggacggcg ttaatcgcgt cgcacctatt
     5281 actaccggtg atgtggtact gcaaagcgcg cgcagcgtgg cggtcggggt gatgctcggt
     5341 atcgacccgg cgcaaaaaga tccacttacg ccgtatctgg tcaatgtgaa acaaactgac
     5401 ctcgagccgg ggaaatataa tgtcatcctc ggcgaacaac ttgcctcaca gctaggcgtt
     5461 aatcgcggtg atcaaatccg cgtgatggta ccatctgcca gccagttcac gccgatgggg
     5521 cgtattccaa gccagcgcct gttcaatgtg attggcaatt tcgccgccaa cagtgaagtc
     5581 gatggctatg aaatgctggt gaatattgag gatgcctcgc gtctgatgcg ttatccggca
     5641 ggcaatatta ccggctggcg tttgtggctg gatgagccgc tgaaagttga ctctttaagt
     5701 cagcaaaaac tgcctgaagg cagcaaatgg caggactggc gtgaccgtaa aggcgagctg
     5761 ttccaggccg tacgcatgga aaaaaatatg atgggcttgc tgctgagcct gattgtcgcc
     5821 gttgcggcgt ttaacattat tacctcacta gggctgatgg taatggagaa gcagggcgaa
     5881 gtcgcgatcc tgcaaacgca aggcttaact ccgcgacaaa tcatgatggt ctttatggtg
     5941 caaggggcca gcgccgggat tatcggtgcg atcctcggag cggcgcttgg cgcactgctt
     6001 gccagccagt taaataatct gatgccgata atcggcgtcc tgcttgatgg cgcggcgctg
     6061 ccggtcgcta tcgaaccttt acaggtcatt gttattgcgc tggtggcgat ggctatcgcg
     6121 ctgctgtcta cgctttaccc ttcatggcgc gctgccgcca ctcaacccgc tgaggcttta
     6181 cgttatgaat aagatcctgt tgcaatgcga caacctgtgc aaacgctatc aggaaggcag
     6241 tgtgcaaacc gatgtactgc acaatgtcag tttcagcgtc ggcgaaggtg aaatgatggc
     6301 gatcgtcggt agctctggtt ccggtaaaag taccttgctg cacctgctgg gcgggctgga
     6361 tacaccaacc tccggcgatg tgatctttaa cggtcaaccg atgagcaaac tgtcttcggc
     6421 ggcgaaagca gaactgcgca accagaagct gggctttatt tatcagtttc accacctgct
     6481 gccggatttt actgccctgg aaaacgtggc tatgccactg ctgattggca agaaaaagcc
     6541 cgctgaaatc aacagccgtg cgctggagat gttaaaagcg gtggggctgg agcatcgtgc
     6601 gaatcaccgc ccatctgaac tttctggcgg cgaacgccag cgtgtggcga ttgcccgtgc
     6661 gctggtcaat aatccgcgtc tggtacttgc ggatgagcct accggtaacc tcgatgcgcg
     6721 taacgccgac agcatcttcc agttgcttgg ggaattgaat cgcttgcagg gcaccgcctt
     6781 cctggtggtt actcacgacc tgcaactggc gaaacgtatg agccgccaac tggaaatgcg
     6841 tgatggacgt ctgacggcgg aactgagcct gatgggggcg gagtaatggc gatgccttta
     6901 tcgttattga ttggcctgcg ttttagtcgc ggacggcgac gcggcggcat ggtgtcgcta
     6961 atctctgtca tttctaccat tggcattgcc ctcggcgtgg cggtattgat cgtcggctta
     7021 agcgcgatga acggctttga acgcgaactg aataaccgca ttcttgctgt ggtaccgcac
     7081 ggtgaaattg aggcggtaaa tcagccatgg actaactggc aggaagcact ggataacgtg
     7141 caaaaagtgc cgggtattgc cgccgctgcg ccgtatatca atttcaccgg actggtagaa
     7201 agtggtacca atctgcgcgc aattcaggtg aagggcgtta acccgcaaca ggaacagcgt
     7261 ctgagcgcat taccctcgtt tgttcagggg gatgcctggc gcaattttaa agcgggcgaa
     7321 cagcaaatta tcatcggcaa aggcgtggcg gatgcgctga aagtgaagca gggcgattgg
     7381 gtgtcgatca tgattcccaa ctcgaatccc gagcataaac tgatgcagcc aaaacgtgtg
     7441 cgtttgcaca ttgccggtat tttgcagttg agtggtcaac tcgatcacag ttttgccatg
     7501 atcccgctgg cggatgccca acaatatctt gatatgggtt ccagcgtgtc aggtattgcc
     7561 cttaaaatga cggatgtttt caacgccaat aagctggtac gcgatgcggg tgaagtgacc
     7621 aacagctatg tttatattaa aagttggatt ggtacttacg gctatatgta tcgcgatatc
     7681 cagatgatcc gcgccattat gtatctggcg atggtactgg tgattggcgt ggcctgtttc
     7741 aacatcgtct ccaccttagt gatggcggtg aaagacaaga gtggcgatat cgcagtatta
     7801 agaacgctgg gggcgaaaga tggtttaatt cgcgccatct ttgtctggta tggattgctg
     7861 gcagggctgt tcggtagcct gtgtggtgtg attatcggcg tagttgtttc actgcaactt
     7921 accccgatta ttgagcggat tgaaaagctg atcggtcatc agttcctctc cagcgatatc
     7981 tattttattg acttcctgcc atcggaattg cactggctgg acgtcttcta cgtactggtc
     8041 acagcattgt tgctgagtct tttggcaagt tggtatccgg cgcggcgcgc cagtaatatt
     8101 gaccctgcgc gagtccttag cggccagtaa aggcagtaca ttaaaacaag gagcggcaat
     8161 gtattacggg tttgatattg gtggaacaaa aattgcgctt ggcgtgtttg atagcggtcg
     8221 gcagttgcag tgggaaaagc gggtgccgac accgcgtgac agctatgacg catttttaga
     8281 tgcagtatgc gagctggtag ccgaagctga ccaacgtttt ggctgtaaag gttctgttgg
     8341 catcggtatt ccgggaatgc cggaaacaga agatggtacg ctgtatgccg ccaatgtccc
     8401 cgctgccagc ggtaaaccgc tgcgtgccga cctgagcgca cgtcttgatc gcgatgtacg
     8461 ccttgataac gatgccaact gttttgccct ttcagaagcc tgggatgatg aatttacgca
     8521 atatccactg gtgatggggt taattctcgg caccggcgtt ggcggcgggc tgattttcaa
     8581 cggtaaaccg attaccggta aaagctacat taccggcgag tttggccata tgcgtctgcc
     8641 ggttgatgcg ttaaccatga tggggctgga tttcccgtta cgccgctgcg gctgtggtca
     8701 aattggttgt atcgaaaatt atctatcagg tcggggcttt gcgtggctct ggcaacacta
     8761 ttatcatcaa ccgttgcagg ctcccgaaat tattgcgctt tatgatcaag gcgatgagca
     8821 ggcaagggcg cacgttgagc gttatctgga tttattagcg gtttgtctgg gaaatatcct
     8881 gaccatcgtt gactctgacc tggtcgtcat tggtggtggc ttatcgaatt tcccggcaat
     8941 cacaacgcaa ctggcggaca ggctgcctcg tcatctctta cctgtagctc gtgttccgcg
     9001 cattgaacgc gcgcgccacg gtgatgcggg aggaatgcgt ggtgcggcct tcctacatct
     9061 aaccgattaa acaacagagg ttgctatgct gtcgcgtcgg ggtcatcggt taagtcgttt
     9121 tcgtaaaaat aaacgccgcc tgcgcgagcg tttgcgtcag cgtatttttt tcagagataa
     9181 agtggtgccg gaagcaatgg aaaaaccaag agtactcgta ctgacagggg caggaatttc
     9241 tgcggaatca ggtattcgta cctttcgcgc cgcagatggc ctgtgggaag aacatcgggt
     9301 tgaagatgtg gcaacgccgg aaggtttcga tcgcgatcct gaactggtgc aagcgtttta
     9361 taacgcccgt cgtcgacagc tgcagcagcc agaaattcag cctaacgccg cgcatcttgc
     9421 gctggctaaa ctgcaagacg ccctcggcga tcgctttttg ctggtaacgc agaatataga
     9481 caacctgcat gaacgcgcag gtaataccaa tgtcattcat atgcatgggg aattgctgaa
     9541 agttcgttgt tcgcaaagtg gtcaggttct cgactggacc ggagacgtta cgccagaaga
     9601 taaatgccat tgttgccagt tcccggcacc cttgcgcccg cacgtcgttt ggtttggcga
     9661 aatgccactc ggcatggatg aaatttatat ggcgttgtcg atggctgata ttttcattgc
     9721 cattggtact tccgggcatg tttatccggc ggctgggttt gttcacgaag cgaaactgca
     9781 tggcgcgcac accgtggaac tgaatctcga accaagtcag gtcggtaatg aatttgccga
     9841 gaaatattac ggcccggcaa gccaggtggt gcctgagttt gttgaaaagt tgctgaaggg
     9901 attataaagc aggaagcatt gcctgatgcg ctacgcttat caggcctaca aggggaccac
     9961 aataccgacg aaagagcggg ataaaacttt actgtcctga ctcattagcc acatccttgc
    10021 taactaaaaa acgggcggta ataccaccgc ccgtttgctg aattaacgtc ctgctttcag
    10081 cttctgataa tactcttcat aaatgctgct ggctgcgcca acgtcattct gccattcacc
    10141 atttttaatg gtttcagcat ccgggtagag cgttttatcg ttcgccactt ccgggcttaa
    10201 cagcttacgc gccgcaaggt ttggcgttgg ataaccgata gtttcggcaa cctgttttgc
    10261 cacatccggg cgcagcagga agttgatcaa tttcagcgcg ccttctttgt ttttggcatt
    10321 tgccgggatc gccaggctgt ccatccagaa aatgccgcct tctttcggcc acaccacgtc
    10381 aattggcgta cccgcctggc gtgcaacgaa agcagagccg ttccagatca tgccgaggtt
    10441 aacttcgcct tccatgtacg ggttagccgg gttatcggag ttaaacgctg cgacgtttgg
    10501 catcagtttt ttcagctcgt tatatgcagc ttcaatctct ttcggatcgg tggtgttacc
    10561 ggagtagccc agtttgcgca gcgccatctg gaacacttca cgggcatcgt cggtcagcag
    10621 caggctacct ttgtactctg gcttccacag atctgcccaa ctggtgacag atttaggatc
    10681 caccgcatca ccgttaacgc caatcgccgt cgcaccccag atatacggaa tggagtagtc
    10741 gttattcggg tcaaacggct tgttgagcat gtctggatcg agattgctga agttcgttaa
    10801 cttcgacttg tcgatcttct ggatcatccc ttctttacgc attttatcga cgtaataggt
    10861 tgaaggaacc accaggtcat aggcaccgtc tttgtacgtt ttcagcttgg cgtacatggt
    10921 ttcgttcgac tcgtaagtcg aatagatagc cttaataccg gtttctttgg tgaactgttc
    10981 aagcagtcct ggcggcacgt actcggtcca gttgtagaaa tacagcgtgt tgttgtcatc
    11041 ggcgtgagcg gcgctcatgc ccagtgccag agcacccgcc gcgagcaggt ggcgtgacca
    11101 ttttttcatt taacgtcccc tgtgttacct ttcgttttat cacgagcaat aagctggctg
    11161 gcaataacca tcaccagcga cagcaccagt aatatggttg ccagcgcgtt aacttccggc
    11221 gatacgccga ctttgaccat cgaataaatt tttaacggca gaatttcata acttggtccg
    11281 gtgacgaacg aagaaactac cacatcgtcc atcgacaggg taaagcttaa cacccagccc
    11341 gccgccaccg ctggcattgc cagtggcaga atgatttttc gcagaatggt aaattcactg
    11401 gcaccgagat ctttcgccgc ttccagcatc cgcacgtcaa aacctttcag gcgcgaatac
    11461 accgtcacca ccacaaacgg caggcagaag gtgatatgcg agaacagcag cgaccagaag
    11521 ccaagctgaa tacccagcag cataaacagc accagcagag aaatcgccat cacgatatct
    11581 ggtgacatca tcaccacaaa cagcattccg ctaacgaacg gcttaccacg aaagcgataa
    11641 cggtacagcg caactgccgt cagtgaaccg ataagcgtag caaatgtcgc cgaaaacacc
    11701 gccattgtta gtgaatgctg cgctgcctgt aacaggctgt cgttgttcat cagcaggcta
    11761 taccatttgg tggtaaaacc ctgccagttg atgccaaagc gcgagctgtt aaaggagttc
    11821 acaatcaaaa taatgattgg gatatacagg tacgcgtaga tagcggtcat aaaaccgccg
    11881 cgaagcagtc gaccgatcat tcgagttcca cctttttatt cagcagacga gaagcgcgcc
    11941 agtaaaccag caacatcagg cccattacga tagtcagcgt aatgctggtg gctgcaccaa
    12001 acggccagtc acgaatattg aggaactgga ccttgatgac gttaccgatc agcaggtttt
    12061 tcgcaccgcc catcaggtcg gatacataga acaggcccat cgctggcagc atcaccagca
    12121 gacatccggc aataattccc ggcatcgtca gtggaataat gatacggata aaagtctgta
    12181 acttgctggc accgagatcg cgcgccgcct caagcaacgg cttatccagt ttttcgatac
    12241 tggagtacag cggcatcacc ataaacggta gcagaatgta aaccagaccg ataatcaccg
    12301 cactgggcgt gaacatgata cgaattggtg tgtcgataac gcccagccag agcaaaaact
    12361 cgttgagata gcctttggtg ctgaggaaaa ttttcagccc gtagatgcga atcagtgagt
    12421 tggtccagaa gggaacaatc agcagaaaca gcagcagcgg acgcaccttg tgtggcagct
    12481 tcgccagaaa ccaggcaaac gggtagccca gcaccaggca ggcgagggtg gcgatcagcg
    12541 ccatattcag cgagtgcaat agcacttcaa aatagagcgg atcgagcaga cgcgtgtagt
    12601 tatccagcgt aaagaccatt ttgacgaaac tggcgtcgtc gcgggtcaaa aagctggtgc
    12661 caatgatcat caggttgggc agaaagacaa acaacacaag ccaaccgaca atagtgacaa
    12721 tcactacatt ctggaactta cttgtgttct tcatcagcca gtacgacctc ccagctttct
    12781 acccaattaa tggccatttt ttggtcgaga gagtggtcaa agtcagggtc gtcttcattg
    12841 aagaattcgc tgaccatcac catcttgcca ttttccagtt caacaaccga ctccagcgtc
    12901 atgcctttgt agttacgctc acgaacgtaa ccaatcagcc cttcagcgtg gttgtcgtcg
    12961 ttaatctctt caacacgtaa gtcttccggg cgcagcagaa catgcagttt ttgccccggt
    13021 tcaacggcga agttaacgta gatattacat tcgcggcctt caacgttggc gcgtacgcgc
    13081 tgctcgtcga gacgttcgat gacagtggcg ttaaacatat tgatttcgcc aatgaagccg
    13141 gcaacaaaca ggtttttcgg ctcttcgtaa atttcgcgcg gtgtaccatc ttgctcaata
    13201 cgaccctcgc gcatcaccac aatcctgtct gacatggtga gcgcttcttc ctgatcgtga
    13261 gtgacaaaga cgaaggtaat accaagctta cgctgtaacg ctttcagctc gttctgcatt
    13321 tgcttacgca gtttgtaatc cagcgctgag agcgactcat ccagcaacaa cagacgaggc
    13381 ttgttaacca ccgcgcgagc aatggcgacg cgttgctgtt gaccaccaga gagctgatgc
    13441 ggtttacgtt gagcgaaggt ttccagttgc accatccgca gggcttccat cacgcggggc
    13501 gtaatttcag cagcgggggt tttttgcatg cgcaacccaa aggccacatt ttcgaacacg
    13561 gtcatgtggg ggaaaagtgc gtagctttgg aaaacagtgt tcacatagcg gttttccgcc
    13621 ggaacgtggg tgatgtcctc gttatccagc atgatgcgtc cggaatcaac agtttccaga
    13681 cctgcaatca ggcgaagaac ggttgtttta ccgcagccag aagggccaag cagcgtgagg
    13741 aactcgccat tgttgatagt cagatccagc tggggaatga cctctttacc atcaaagcat
    13801 ttgcgaattc ccgccaattg caccagcggt gaaagcgaac tcggttgttt attcaatttt
    13861 ttactctgtc ccatgtaaac gcaacggatg gcttaccgat gcggggtttg tggttaacca
    13921 ccttggtgac tcttaatgag ggcggtaatt ctacggcaaa ccgcttgaat cgccaatctt
    13981 tgttgtgaat tactggctta gctttatatt cattaaggta atgctgataa atattcccgc
    14041 ttgcaggggt aaaagtgacc tgacgcaata tttgtctttt cttgcttctt aataatgttg
    14101 tcacaaaaag tgagggtgac tacatggata aactacttga gcgatttttg aactacgtat
    14161 ctctggatac ccaatcaaaa gcaggggtga gacaggttcc cagcacggaa ggccaatgga
    14221 agttattgca tctgctgaaa gagcagctcg aagaaatggg gcttatcaat gtgaccttaa
    14281 gtgagaaggg cactttgatg gcgacgttac cggctaacgt ccctggcgat atcccggcga
    14341 ttggctttat ttctcatgtg gatacctcac cggattgcag cggcaaaaat gttaatccgc
    14401 aaattgttga aaactatcgc ggtggcgata ttgcgctggg tatcggcgat gaagttttat
    14461 caccggttat gttcccggtg ctgcatcagc tactgggtca gacgttgatt accaccgatg
    14521 gtaaaacctt gttaggtgcc gatgacaaag caggtattgc agaaatcatg accgcgctgg
    14581 cggtattgca acagaaaaac attccgcatg gtgatattcg cgtcgccttt accccggatg
    14641 aagaagtggg caaaggggcg aaacattttg atgttgatgc cttcgatgcc cgctgggctt
    14701 acactgttga cggtggtggc gtaggcgaac tggagtttga aaacttcaac gccgcatcgg
    14761 tcaatatcaa aattgtcggt aacaatgttc atccgggcac ggcgaaagga gtgatggtaa
    14821 atgcgctgtc gctggcggca cgtattcatg cggaagttcc ggcggatgaa agcccggaaa
    14881 tgacagaagg ctatgaaggt ttctatcacc tggcgagcat gaaaggcacc gttgaacggg
    14941 ccgatatgca ctacatcatc cgtgatttcg accgtaaaca gtttgaagcg cgtaaacgta
    15001 aaatgatgga gatcgccaaa aaagtgggca aagggttaca tcctgattgc tacattgaac
    15061 tggtgattga agacagttac tacaatatgc gagagaaagt ggttgagcat ccgcatattc
    15121 tcgatatcgc ccagcaggcg atgcgtgact gcgatattga accggaactg aaaccgatcc
    15181 gcggtggtac cgacggcgcg cagttgtcgt ttatgggatt accgtgcccg aacttgttca
    15241 ctggcggtta caactatcat ggtaagcatg agtttgtgac tctggaaggt atggaaaaag
    15301 cggtgcaggt gatcgtccgt attgccgagt taacggcgca acggaagtaa gcgaaaaggg
    15361 atgcggcatg tgatgccgca tccggcttaa atccaaactt acccttcgaa gaaccaatac
    15421 ccgctattga ccagcgccgc gagcatcgcg aggaatgacg gatcttccag cgcatcgcca
    15481 aaattctccg cagtcagcgc aatgttgctg gcgagtgcat ccagtgccgg acggtgcggg
    15541 gaatcgatct tctcaccatt ggcatacacg tcgtcgccaa tgcgcaatac gcgcagacca
    15601 cccaggcgca ccagcacatc accttgtttc agcgcatcgt agatttcatc tggctgataa
    15661 ggcggttctg gcggcgcgat atccagttca tgacgtgact gggatataaa ctcgccaaac
    15721 cattgcttaa agtgttccgg ctggttgatc aattcgagca tcatctcacg cagtttatcc
    15781 atctcttgag gcagaacatc cgcaggatga gcgcgaggtg gaacatcagg atcgctgtag
    15841 tagttgccgc ccagttcacg ttgcagcaca taatcggcaa atccactaat cagttcccgc
    15901 gtatttggcg cgcgaaagcc cacggaatag ttcatcgcat tttccagcgc gtagccttca
    15961 tgcgggaatc ctggcggaat ataaagaata tcaccaggct ccagctcttc atcgatgatg
    16021 gcttcgaacg gatcgacctg taacagatcc ggatgtgggc agtgctgttt catttgcagc
    16081 ttttcgccca ctcgccagcg acgacgtccg gtaccctgaa tgataaacac gtcgtactga
    16141 tcgagatgcg ggccgacgcc gccgccaggt acagaaaaag aaatcatcag atcatcaata
    16201 cgccagtccg gtagttcacg gaacggtcgc atcagcgcgg cggtcggctc atgccagtgg
    16261 ttcactgcct gtaccagtaa tgaccagttg gtttcaccga gatgatcgta gctttcgaac
    16321 gggccgtggc tgacctgcca tttgccatcc tggtgactga ccagtcgact gtcaacttcg
    16381 ctttccatcg ccagacccgc caactcgtct ggagagatcg ggtcaataaa attattaaag
    16441 ccgcgtttta acaccaccgg gcgtttctgc cagtgacgtt caagaaaatc gggccagtta
    16501 agagtgagtt ggtattccat gttaagcatc cacaggctgg tatctgcaac cgattataac
    16561 ggatgcttaa cgtaatgcgt gaagtatgga catatttatt catctttcgg cgcagaatgc
    16621 tggcgaccaa aaatcacctc catccgcgca ccgcccagca tgctctctcc ggcgacgatt
    16681 ttaccctcat attgctcggt gatttcgcgg gctaccgcca gccctacacc ttgcccaggg
    16741 cgtaaagtat caacccgttg accacggtcg aaaatgacct ctcgcttgct taatggaata
    16801 ccggggccat catcctcgac cacaatatag agatgctcgt cggtttgcct tgcagaaatt
    16861 tcgacaaact cgaggcaata tttacaggca ttatccagca cgttgcccat cacctcgaca
    16921 aaatcgttct gctcaccgac aaagctgatc tctggcgaaa tatcgagaga gatattgacc
    16981 cctttgcgtt gatacacttt gttcagcgct gaggtgagat tgtccagcag tggggcgacc
    17041 ggatgcagct cgcggctgag caatgtcccg ccgcgcatac tggcacgatg caggtagtag
    17101 ccaatttgct gtgaaatgcg gctgatttgc tccagcatta ccggctcagc atcactgacg
    17161 ctcatctttt cactacgcag agaacgcagc gtactttgca gcaccgccag tggcgttttc
    17221 agactatggg tcaggtcggt gagcgtcgta cggtatttgt cgtaacgttc gcgttcactt
    17281 tttaacaatc ggttcaggtt tcgtaccaga ctggtcagtt ctcgcgttgt ggctggattg
    17341 agcaattcgc ggttatgttc ttccagttcg cggacttctt ttgccagggc ttcgatgggg
    17401 cgtaaactcc accaggcggc gacccacagc agcgggatca ctaacagcag attggctgag
    17461 agcacataga taaaccagct ccagaccata taggaacttt ttagctccac cggaatggta
    17521 tccaccacca caatggttaa ttttggcatc cgcgatgttg ccgggtagac gtttactgcc
    17581 accgagtggg tcatctccgc gtcgtcatca tcttcccgca cttcctgcaa ctgttgctgt
    17641 atcgaatgat ctccactcag caagaggctg gtatcgttaa catccgcttc aatttcatga
    17701 aaaccattcg atttcagcca gtcaggctgg atcatcttca tcagccaggg cacgtcacgt
    17761 tgcgcccata aaagctgccc gttctcatca taaattagcg tcatggtggg gctttgcttg
    17821 tcgatatttt cgggtaactc gacatgcaac ttattgtttt cccacttcgc aagggtatag
    17881 aacagattgc tctcgccacg taacagccga aacgtagttt tatcgaaact gacgctataa
    17941 ccgatcagcg cgaccattcc gtaggcaagc gaaagcacca gtactaccgc agccgttgcc
    18001 aacagaaaac gtacccgcag cgagagcggg aaaaaaagac gcagtaattt tttcatcagc
    18061 gcaattcgaa caaatagccc tggccgcgaa cggtagtaat cacttcttgc ggatattgtg
    18121 cctgaatttt tttgcgcaga cgtcccatca gtacatcaat ggtatggctt tcccgcagct
    18181 ccgcatccgg atagagttgg agcattaacg aatctttgct caccactttg ccattattgc
    18241 gtatcaacgt ttccataatg gtgtattcga acgcggtcag tttgatcact tcgtcattaa
    18301 tagataattc acggcgagag agatcaacct gaaacggtgg tagcgaaatg acctgtgaag
    18361 ccagaccgct attacgccgc attaatgcct gcattcgcgc catcacctct tcaatatgaa
    18421 atggtttagt cacgtaatca tcagcaccag cacttaatac ttcgactttg tcctgccagc
    18481 tttcacgggc ggttaatacc agaatcggca gtgaaacatc attgctacgc cagcggcgaa
    18541 tcagtgacag accgtcctcg tctggcaatc cgagatcgac aatcgcaata tccggtaaat
    18601 gttcattgag ataataatcg gcttctttgg catcttctgc atcatcgacc tgatgaccag
    18661 catcctgaat ctgaacttta aggtggtgac gtaacaacgc attgtcttca acaaccagta
    18721 cgcgcatttt tatttctccc tgtcttaatt attaaaatag tgtaacgcga ttatgtggtt
    18781 atgggggtaa acattaaata aaccagcggg gaggggaggt aaagtgaaaa aataaaaagc
    18841 ggataatctt aataagcagg ccggacagca tcgccatccg gcactgatac gagtttattt
    18901 cagctcatca accatggtga tggcgcgacc aatgtagttt gccggcgtca tcgctttcag
    18961 gcgggctttc tcttcttctg gcaacgccag accgtcgata aactgcttca tgccttcggc
    19021 gtcaacgcgc ttaccacgag tcagctcttt cagcttctcg tacggttttt cgatgccata
    19081 gcgacgcata actgtctgga ttggctcagc cagcacttcc cagttgtgat ccagctcatc
    19141 cagcagatgg tcacggttca cttccagttt gctcacgcct ttcagggtgg attgatacgc
    19201 aatcagcgca taaccgatac ccacgccgag gttacgcagc acggtggagt cggtcaggtc
    19261 acgctgccag cgggaaaccg gcagtttgct tgccagatgc tgtaataccg cgttggaaag
    19321 gcccagattc ccttcggagt tctcgaagtc gatcgggtta actttatgcg gcatggtgga
    19381 agaaccaatc tcaccagcaa tggttttctg tttgaagtgg ttaagggcga tataacccca
    19441 gacgtcacga tcaaagtcga tcagaatggt gttgaagcgt gcaacgcaat caaacagctc
    19501 ggcgatgtag tcgtgcggtt caatctgggt agtgtacggg ttccattgaa tacccagcga
    19561 ggtgacgaac tcttcgctga actgatgcca gtcaacttcc gggtaagcgg cgatgtgggc
    19621 gttatagtta ccgaccgcgc cgttgatttt gccgaggatc tccacctggt taagctggcg
    19681 gtactggcgc tccatacggt aggcgacgtt tgccatctct ttaccgatgg ttgacggcgt
    19741 ggctggctga ccgtgggtac gagacagcag cgggatatcg cggtactgaa cggcgagatc
    19801 tttaatgcca tcaatcagtt gacgccagta cggcaggatc acttcatcac gcgcggtttt
    19861 cagcattaat gcgtgggaga ggttattgat atcttccgaa gtacaggcaa agtggatgaa
    19921 ttcagaaacc gcgtgcagtt ccgggatctc cgccactttt tctttcagga aatactcaac
    19981 cgctttaacg tcgtggttag tggtacgctc gatggttttg atgcgtgcgg catcttcttc
    20041 gctgaaattg gcgacaattg catcaaggta accgattgcg tcggcagcaa aagcaggaac
    20101 ttccttgatc gctgcgtgcg cggccagttt ttgcagccaa cgtacttcaa cttgtacacg
    20161 gaatttcagc aaaccatatt cgctgaaaat cccgcgcagc gcgctgactt tatcgccgta
    20221 gcgtccatcg acaggggaaa cggcggtcag tgaggataat tccatagatc acaactccgg
    20281 ggttaaatga gcaagaattt gttttgcctg agtggtcagg cgattacgag aaaacatcag
    20341 ttgcagacgt ccgccgccga cctggtgcca gagcacggcg gcgcgaatgc ctgccagcag
    20401 ggttgcgcga actttcgcct gcacttgtgg gctttgcagt acagcagggg aaccggtgac
    20461 ctgaatgcgt gggccaagcg ggctgatcac atcaacatag atagcagcca tcgcgctcat
    20521 cagcgtttcg gactgtaaat cgaagtgttc gagctggcgt tgcaggccgt tgatccggtt
    20581 gcccagagtg tcgagcgcgc ctttcgctga ggagagtttg cgctcaagca ccatcaagct
    20641 gagtgtgtag cgggttaatt cggcgtttaa gccctggcga ctgctggcat tgagcacgcc
    20701 gagcagggtt tccagcccga cgcgcaggtt ggcttcgcta ccgccaaaaa ccgccagcgt
    20761 cgagctgggg ttcatatcaa taatactgtt gagtgagacg tgtagcgcat cggcatcaca
    20821 atgcccctgg tgagcgagtt gttgcaccag gcgtgccgac tgacaaatac cggccagggc
    20881 gagggtgatg tcatagtaat tctttgccac gttcactgct tccttgtttt aagtaaagat
    20941 aataatcaga ccggcagcgg cagacgctgc tcaataatac cgccaccgag gcacacttca
    21001 ccgttataga agacggcaga ctggcccggc gtcacggcgg caaccggttc atcgaaaatc
    21061 acttcaatgc gatcatcgtc cagcgccttg acggtgcaag ggatatcggt ctggcgatag
    21121 cgggttttta ccgtgcaacg catagtgccg gtgaatggtt cgcgatcgac ccagtgcaac
    21181 tgctgggcaa tcaacccgac agacatcagc cgcgggtgtt catggccctg agcgacaacc
    21241 agaatgttgt tttcgacgtc tttgtccacc acataccaag gttcttcggt gccttctttg
    21301 gtgccaccga tacccagacc tttacgctga ccgagagtgt gatacatcag cccctggtgc
    21361 tcaccaattt catcgccatc gacggtaatg attttgcccg gttgccccgg gagataacgg
    21421 cccaggaact cgcggaattt acgttcgcca atgaagcaaa tgccggtaga gtcttttttc
    21481 ttcgcggtga ccagaccaag atcttcagca atcttacgca cctgcggttt ttccagttcg
    21541 ccgaccggga acaggctttg cgcaatctgc tcatggctga gcgtataaag gaagtagctc
    21601 tggtctttat tgctgtccag accacgcagc aggcggctct tgccatcgac atcggcccga
    21661 cgtacgtaat gaccggtagc gatataatcg gcacctaaat cttcggcggc aaattcgagg
    21721 aaagctttaa atttgatctc tttgttgcac agaatatccg gattcggcgt gcgaccggct
    21781 ttatattcgg caaggaacag ttcgaagaca ttgtcccagt actcggcagc aaagttaacg
    21841 gtgtgcagtt caatgccgag cttgtcgcag acagcctggg catcagccag atccgctgcc
    21901 gctgtgcaat attcctcacc gtcgtcttct tcccagttct tcataaacag gccttcaacc
    21961 tgatatccct gttgttgcaa cagccaggca gaaacggagg aatcgacacc gccggacatg
    22021 ccgacgatta ctttttttgc ggtttcagac attggatcac tcacgacatt gaacttcaag
    22081 gcggcgtatt ctatcacgca gccttttaga tgacaccctt tgtaaaaggc cagttaaaat
    22141 cgccaatcat ctccagcgga tagcgttgcc cactttgata acaacggata ctttccgcca
    22201 ccagcggcga acgaagattt gacgccttta aaatttcttc ggcgctgacc caacgacagc
    22261 agtcgatatc gctgtcatga ggctgggtcg ggcatatttg ctcaagctca atggcaaaga
    22321 ggaaacgcaa aaacggcgtt ttatctggcg caatccactg atgcatacga ataaagtgtt
    22381 gcggttgcgc gctgatgccg gtttcttccc atagctcacg ggcggcagct tccactaagg
    22441 tttcatcggc ttccagatgc ccggcaggtt ggttccataa cgctttacca ttaatcgtct
    22501 cttcaacgac taaaaattta ccctctgcgt gcactacgca agcaacggta acgtgcggtt
    22561 taaacattgt gcatccttat tctgttgctt ctcgccattc gccattggca aggttatcca
    22621 aagtgtaatc acccatcgca taacgaatca gtcgcagcgt ggggaaacca acatgggcgg
    22681 tcatgcggcg cacctggcga ttacgtcctt catataaggt gatcttcagc cagctggtgg
    22741 gaatactttt gcgttcacga attggtggat tccgcggcca taaccacgct ggttcgtcaa
    22801 ccagctccgc gccggcgggc agggtagggc catcatttaa ggttacgcca ttgcgcaagg
    22861 cttcaagtgc gtcttgtgtg ggaatacctt ccacctgcac ataatagatt ttcccggtgc
    22921 gtttgcccgg ctgggttaaa cgcgcctgca acgcgccgtt attggtcagc accagtaacc
    22981 cttcggtatc gcggtcaaga cgacctgctg cataaacgcc ctgaaccggg atgaattctt
    23041 ttaatgtttt gcgtccggct tcatcggtga actgcggaag aacatcgtag ggtttattga
    23101 acaggatcac acgcgtgggc tggttttcag gtttacgcct agtagaacgt tgcgagctga
    23161 atcgcttaac ctggtgattt ctaaaagaag ttttttgcat ggtattttca gagattatga
    23221 attgccgcat tatagcctaa taacgcgcat ctttcatgac ggcaaacaat agggtagcat
    23281 tgacaagcca attacaaatc attaacaaaa aattgctcta aagcatccgt atcgcaggac
    23341 gcaaacgcat atgcaacgtg gtggcagacg agcaaaccag tagcgctcga aggagaggtg
    23401 aatggaaagt aaagtagttg ttccggcaca aggcaagaag atcaccctgc aaaacggcaa
    23461 actcaacgtt cctgaaaatc cgattatccc ttacattgaa ggtgatggaa tcggtgtaga
    23521 tgtaacccca gccatgctga aagtggtcga cgctgcagtc gagaaagcct ataaaggcga
    23581 gcgtaaaatc tcctggatgg aaatttacac cggtgaaaaa tccacacagg tttatggtca
    23641 ggatgtctgg ctgcctgctg aaacccttga tctgattcgt gaatatcgcg ttgccattaa
    23701 aggtccgctg accactcctg ttggtggcgg tattcgctct ctgaacgttg ccctgcgcca
    23761 ggaactggat ctctacatct gcctgcgtcc ggtacgttac tatcagggca ctccaagccc
    23821 ggttaaacac cctgaactga ccgatatggt tatcttccgt gaaaactcgg aagacattta
    23881 tgcgggtatc gaatggaaag cagactctgc cgacgccgag aaagtgatta aattcctgcg
    23941 tgaagagatg ggggtgaaga aaattcgctt cccggaacat tgtggtatcg gtattaagcc
    24001 gtgttcggaa gaaggcacca aacgtctggt tcgtgcagcg atcgaatacg caattgctaa
    24061 cgatcgtgac tctgtgactc tggtgcacaa aggcaacatc atgaagttca ccgaaggcgc
    24121 gtttaaagac tggggctacc agctggcgcg tgaagagttt ggcggtgaac tgatcgacgg
    24181 cggcccgtgg ctgaaagtta aaaacccgaa caccggcaaa gagatcgtca ttaaagacgt
    24241 gattgctgat gcattcctgc aacagatcct gctgcgtccg gctgaatatg atgttatcgc
    24301 ctgtatgaac ctgaacggtg actacatttc tgacgccctg gcagcgcagg ttggcggtat
    24361 cggtatcgcc cctggcgcaa acatcggtga cgaatgcgcc ctgtttgaag ccacccacgg
    24421 tactgcgccg aaatatgccg gtcaggacaa agtaaatcca ggctctatca tcctctccgc
    24481 tgagatgatg ctgcgccata tggggtggac tgaagcggct gatttaattg ttaaaggtat
    24541 ggaaggtgct atcaacgcga aaaccgtaac ttatgacttt gaacgtctga tggaaggcgc
    24601 taagctgctg aaatgttcag agtttggtga agcgatcatc gaaaacatgt aatctctcca
    24661 tgagttaaat attgaaacgg gcgtataaca cgcccgttgt tttatttatg tggatattat
    24721 taatagcata tcga
//
LOCUS       DAZPJR010000051        24717 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPJR010000051 DAZPJR010000000
VERSION     DAZPJR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.052"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..164)
                     /locus_tag="ACHVB7_001894"
     CDS             complement(<1..164)
                     /locus_tag="ACHVB7_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR1913896.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTV"
     gene            complement(222..455)
                     /locus_tag="ACHVB7_001895"
     CDS             complement(222..455)
                     /locus_tag="ACHVB7_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR1913897.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            844..1389
                     /locus_tag="ACHVB7_001896"
     CDS             844..1389
                     /locus_tag="ACHVB7_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR1913898.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1364..3289
                     /locus_tag="ACHVB7_001897"
     CDS             1364..3289
                     /locus_tag="ACHVB7_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1913899.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3286..3492
                     /locus_tag="ACHVB7_001898"
     CDS             3286..3492
                     /locus_tag="ACHVB7_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR1913900.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3489..5090
                     /locus_tag="ACHVB7_001899"
     CDS             3489..5090
                     /locus_tag="ACHVB7_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1913901.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5071..6390
                     /locus_tag="ACHVB7_001900"
     CDS             5071..6390
                     /locus_tag="ACHVB7_001900"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1913902.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6400..6732
                     /locus_tag="ACHVB7_001901"
     CDS             6400..6732
                     /locus_tag="ACHVB7_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR1913903.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            6788..7813
                     /locus_tag="ACHVB7_001902"
     CDS             6788..7813
                     /locus_tag="ACHVB7_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1913904.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7855..8253
                     /locus_tag="ACHVB7_001903"
     CDS             7855..8253
                     /locus_tag="ACHVB7_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1913905.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8265..8618
                     /locus_tag="ACHVB7_001904"
     CDS             8265..8618
                     /locus_tag="ACHVB7_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1913906.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8630..9208
                     /locus_tag="ACHVB7_001905"
     CDS             8630..9208
                     /locus_tag="ACHVB7_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1913907.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9205..9600
                     /locus_tag="ACHVB7_001906"
     CDS             9205..9600
                     /locus_tag="ACHVB7_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1913908.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            9608..10348
                     /locus_tag="ACHVB7_001907"
     CDS             9608..10348
                     /locus_tag="ACHVB7_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1913909.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10364..10786
                     /locus_tag="ACHVB7_001908"
     CDS             10364..10786
                     /locus_tag="ACHVB7_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1913910.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            10768..11202
                     /locus_tag="ACHVB7_001909"
     CDS             10768..11202
                     /locus_tag="ACHVB7_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1913911.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11195..13756
                     /locus_tag="ACHVB7_001910"
     CDS             11195..13756
                     /locus_tag="ACHVB7_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1913912.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            13753..14082
                     /locus_tag="ACHVB7_001911"
     CDS             13753..14082
                     /locus_tag="ACHVB7_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1913913.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14082..14780
                     /locus_tag="ACHVB7_001912"
     CDS             14082..14780
                     /locus_tag="ACHVB7_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1913914.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14786..15529
                     /locus_tag="ACHVB7_001913"
     CDS             14786..15529
                     /locus_tag="ACHVB7_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1913915.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15526..16098
                     /locus_tag="ACHVB7_001914"
     CDS             15526..16098
                     /locus_tag="ACHVB7_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1913916.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16159..19656
                     /locus_tag="ACHVB7_001915"
     CDS             16159..19656
                     /locus_tag="ACHVB7_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR1913917.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            19727..20243
                     /locus_tag="ACHVB7_001916"
                     /pseudo
     CDS             19727..20243
                     /locus_tag="ACHVB7_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20308..23391
                     /locus_tag="ACHVB7_001917"
     CDS             20308..23391
                     /locus_tag="ACHVB7_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1913918.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            23403..23972
                     /locus_tag="ACHVB7_001918"
     CDS             23403..23972
                     /locus_tag="ACHVB7_001918"
                     /inference="COORDINATES: protein motif:HMM:NF014467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1913919.1"
                     /translation="MSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTDIAPP
                     DIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSPEGEF
                     QKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKETLLL
                     EAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         5931 a         6115 c         7469 g         5202 t
ORIGIN      
        1 aaaaccgtat tatcgttttc aagaacaaga tgaatattct ctgtggctaa atagtaaatg
       61 taatgtgaga cattgtgacg ttttagttca gaataaaacc agtgatagtt taaattattt
      121 cgcactttat cgaatatttg tttaaaaatg gcaacctgag ccattgtagt accttccatg
      181 tgatatgagg gggcgtagtc tgcacgatta tctaaattgc ttcaatctgg tctaacctgt
      241 tttctgagca attcagtaat gtcactcttt tctttgtttg cttcaggcga aactcttttt
      301 tctgagcaca gtctccggcg gcaggcttca atgacccagg ctgagaaatt cccggaccct
      361 ttttgaacaa gagcgatgtt aatttgttca atcatttggt taggaaagcg gatgttgcgg
      421 gttgttgttc tgcgggttct gttcttcgtt gacatgaggt tgccctgtat tcagtgtcgc
      481 tgatttgtat tgtctgaagt tgtttttacg ttaagttgat gcagatcaat taatacgata
      541 cctgcgtcat aattgattat ttgacgtggt ttgatggcgt agatgcacgt tgtgacatgt
      601 agatgataat tattatcatt ttgcgggtcc tttccggcga tccgacaggt tacggggcgg
      661 aaggcgcgcg ggttttcgct atttatgaca attttccggt ttaaggcgtt tccgttcttc
      721 ttcgccgtaa cctaatgttt ttatttaaaa caccccctga aaagaaagga aacgacaggt
      781 gctgaaaacg ggctttttgg cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga
      841 acaatggaag tcaacaaaaa gcagctggct gacattttcg gtgcgagtat ccgtaccatt
      901 cagaactggc aggaacaggg aatgcccgtt ctgcgaggcg gtggcaaggg taatgaggtg
      961 ctttatgact ctgccgccgt tataagatgg tatgccgaaa gggatgctga aattgagaac
     1021 gaaaagctgc gccgggaagt tgaagaactg cggcaggcca gcgagacaga tctccagcca
     1081 gggactattg agtacgaacg ccatcgactt acgcgtgcgc aggccgacgc acaggagctg
     1141 aaaaatgcca gagactccgc tgaagtggtg gaaaccgcat tctgtacttt cgtgctgtcg
     1201 cggatcgcag gtgaaattgc cagtattctc gacgggatcc ccctgtcggt gcagcggcgt
     1261 tttccggaac tggaaaaccg acatgttgat ttcctgaaac gggatatcat caaagccatg
     1321 aacaaagcag ccgcgctgga tgaactgata ccggggttgc tgagtgaata tatcgaacag
     1381 tcaggttaac aggctgcggc attttgtccg cgccgggctt cgctcactgt tcaggccgga
     1441 gccacagacc gccgttgaat gggcggatgc taattactat ctcccgaaag aatccgcata
     1501 ccaggaaggg cgctgggaaa cactgccctt tcagcgggcc atcatgaatg cgatgggcag
     1561 cgactacatc cgtgaggtga atgtggtgaa gtctgcccgt gtcggttatt ccaaaatgct
     1621 gctgggtgtt tatgcctact ttatagagca taagcagcgc aacacactta tctggttgcc
     1681 gacggatggt gatgccgaga actttatgaa aacccacgtt gagccgacca tccgcgatat
     1741 tccgtcgctg ctggcgctgg ctccgtggta tggcaaaaag caccgggata acacgctcac
     1801 tatgaagcgt ttttccaatg gtcgtggctt ctggtgcctg ggcggtaaag cggcaaaaaa
     1861 ctaccgtgaa aagtcggtgg atgtggcggg ttatgatgaa cttgctgcct ttgatgagga
     1921 tattgaacag gaaggctctc cgacgttcct tggcgacaaa cgtattgaag gctcggtctg
     1981 gccaaagtcc atccgtggct ccacgcccaa agtgagaggc acctgccaga ttgagcgtgc
     2041 agccagtgaa tccccgcatt ttatgcgttt tcatgttgcc tgcccgcact gcggggagga
     2101 gcagtatctt aaatttggcg acaaagagac gccgtttggc ctcaaatgga cgccggatga
     2161 cccctccagc gtgttttatc tctgcgagca taatgcctgc gtcatccgcc agcaggagct
     2221 ggactttact gatgcccgtt atatctgcga aaagaccggg atctggaccc gtgatggcat
     2281 tctctggttt tcgtcatccg gtgaagagat tgagccacct gacagtgtga cctttcacat
     2341 ctggacagcg tacagcccgt tcaccacctg ggtgcagatt gtcaaagact ggatgaaaac
     2401 gaaaggggat acgggaaaac gtaaaacctt cgtaaacacc acgctcggtg agacgtggga
     2461 ggcgaaaatt ggcgaacgtc cggatgctga agtgatggca gagcggaaag agcattattc
     2521 agcgcccgtt cctgaccgtg tggcttacct gaccgccggt atcgactccc agcgggatcg
     2581 ctacgaaatg cgcgtatggg gatgggggcc gggtgaggaa agctggctga ttgatcggca
     2641 gattattatg ggccgccacg acgatgaaca gacgctgctg cgtgtggatg aggccatcaa
     2701 taaaacctat acccgccgga atggtgcaga aatgtcgata tcccgtatct gctgggatat
     2761 tggcgggatt gacccgacca ttgtgtatga acgctcgaaa aagcatgggc tgttccgggt
     2821 gatccccatt aaaggggcat ccgtctacgg taagccggtg gccagcatgc ctcgtaagcg
     2881 aaacaaaaac ggggtttacc ttaccgaaat cggtacggat accgcgaaag agcagattta
     2941 taaccgcttc acactgacgc cggaagggga tgaaccgctt cccggtgccg ttcacttccc
     3001 gaataacccg gatatttttg atctgaccga agcgcagcag ctgactgctg aagagcaggt
     3061 cgaaaaatgg gtggatggca ggaaaaaaat actgtgggac agcaaaaagc gacgcaatga
     3121 ggcgctcgac tgcttcgttt atgcgctggc ggcgctgcgc atcagtattt cccgctggca
     3181 gctggatctc agtgcactgc tggcgagcct gcaggaagag gatggtgcag caaccaacaa
     3241 gaaaacactg gcagattacg cccgtgcctt atccggagag gatgaatgac gcgacaggaa
     3301 gaacttgccg ctgcccgtgc ggcactgcat gacctgatga caggtaaacg ggtggcaacg
     3361 gtacagaaag acggacggcg agtggagttt acggccactt ccgtgtctga cctgaaaaaa
     3421 tacattgcgg agctggaggt gcagaccggc atgacacagc gacgcagggg acctgcagga
     3481 ttttatgtat gaaaacgccc accattccca cccttctggg accggacggc atgacatcgc
     3541 tgcgtgaata tgccggttat cacggcggtg gcagcggatt tggtgggcag ttgcgggcgt
     3601 ggaacccacc gggtgaaagt gtggatgcag ccctgctgcc caactttacc cgtggcaatg
     3661 cccgcgcaga cgatctggta cgcaataacg gctatgctgc caacgccatc cagctgcatc
     3721 aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg
     3781 gcatcgggga ggaagacgcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt
     3841 ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga
     3901 ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg
     3961 ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca
     4021 gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca
     4081 gcggtgcggc gctgggatat tacgtcagcg aggacggcta tcctggctgg atgccgcaga
     4141 aatggacatg gataccccgt gagttacccg gcgggcgcgc ctcgttcatt cacgtttttg
     4201 aacccgtgga ggacgggcag acccgcggtg caaatgtgtt ttacagcgta atggagcaga
     4261 tgaagatgct cgacacgctg cagaacacgc agctgcagag cgccattgtg aaggcgatgt
     4321 atgccgccac cattgaaagt gagctggata cgcagtcagc gatggatttt attctgggcg
     4381 cgaacagtaa ggagcagcgg gaaaggctga cgggctggat tggtgaaatt gccgcgtatt
     4441 acgccgcagc accggtccgt ctgggaggcg caaaagtgcc gcacctgatg ccgggggact
     4501 cactgaacct gcagacggct caggacacgg ataacggcta ctccgtgttt gagcagtcac
     4561 tgttgcggta tatcgctgcc gggctgggtg tctcgtatga gcaactttcc cggaattacg
     4621 cccagatgag ctactccacg gcacgggcca gtgcgaacga gtcgtgggcg tactttatgg
     4681 ggcggcgaaa attcgtcgca tcccgtcagg cgagccagat gtttctgtgc tggctggaag
     4741 aggccatcgt tcgccgcgtg gtgacgttac cttcaaaagc gcgcttcagc tttcaggaag
     4801 cccgcagtgc ctgggggaac tgcgactgga taggctccgg tcgtatggcc atcgatggtc
     4861 tgaaagaagt tcaggaagcg gtgatgctga tagaagccgg actgagcacc tacgagaaag
     4921 agtgcgcgaa acgcggtgac gactatcagg aaatttttgc ccagcaggtc cgtgaaacga
     4981 tggagcgccg cgcagctggt cttaaaccgc ccgcctgggc ggctgcggca tttgaatccg
     5041 ggctgcgaca atcaacagag gaggagaaga gtgacagcag agctgcgtaa tctcccgcat
     5101 attgccagca tggcttttaa tgagccgctg atgcttgaac ccgcctatgc gcgggttttc
     5161 ttttgtgcgc ttgcaggcca gcttgggatc agctgcctga cggatgcagt atccggcgac
     5221 agcctgactg ccggagaggc acccgcggcg ctggcgttat ccggtgatga tgacggacca
     5281 cgacaggccc gcagttatca ggtaatgaac ggcatcgccg tgctgccggt gtccggcacg
     5341 ctggtcagcc ggacgcgggc gctgcagccg tactcgggga tgaccggtta caacggcatt
     5401 atcgcccgtc tgcaacaggc tgccagcgac ccgatggtgg acggcattct gctcgatatg
     5461 gacacaccgg gcggaatggt ggcgggagca tttgactgcg ctgacatcat cgcccgtgtg
     5521 cgtgacataa agccggtatg ggcgctggcc aatgacatga actgcagcgc aggtcagctg
     5581 cttgccagtg ccgcctcccg gcgtctggtc acgcagaccg cccggacagg ctccatcggc
     5641 gtcatgatgg ctcacagtaa ttacggcgct gcactggaga aacagggcgt ggaaatcacg
     5701 ctgatttaca gcggcagcca taaggtggat ggcaacccct acagccatct accggatgat
     5761 gtccgggaaa cattgcagtc ccggatggat gcaacccgcc ggatgtttgc acagaaggtg
     5821 tcggcatata ccggcctgtc cgtgcaggct gtgctggata ccgaggctgc agtgtacagc
     5881 ggtcaggagg ccattgatgc cggactggct gatgaacttg ttaacagtac cgatgcgatc
     5941 accgtcatgc gtgatgcact ggatgcacgt aaatcccgtc tctcaggagg gcgaatgacc
     6001 aaagagactc aatcaacaac tgtttcagcc actgcttcgc aggctgacgt tactggcgtg
     6061 gtgcaagcga cggagggcga gaacgccagc gcggcgcagc cggacgtgaa cgcgcagatc
     6121 accgcagcgg ttgcggcaga aaacagtcgc attatgggga tcctcaactg tgaggaggct
     6181 cacggacgcg aagaacaggc acgcgtgctg gccgaaaccc ccggtatgac cgtggaaacg
     6241 gcccgccgta ttctggccgc agcaccacag agtgcacaga cgcgcagtga cactgcgctg
     6301 gatcgtctga tgcagggggc accggcaccg ctggctgcag gtaacccggc atctgatgcc
     6361 gttaacgatt tgctgaacac accagtgtaa gggatgttta tgacgagcaa agaaaccttt
     6421 acccattacc agccgctggg caatagtgac ccggctcata ccgcaaccgc gcccggcgga
     6481 ttgagtgcga aagcgcctgc aatgaccccg ctgatgctgg acacctccac ccgtaagctg
     6541 gttgcgtggg atggcaccac cgacggtgct gccgttggca ttcttgcggt tgctgctgac
     6601 cagaccagca ccacactgac gttctacaag tccggcacgt tccgttatga ggatgtgctc
     6661 tggccggagg ctgccagcga cgagacgaaa aaacggaccg tgtttgccgg aacggcaatc
     6721 agcatcgttt aactttaccc ttcatcacta aaggccgcct gtgtggcttt ttttacggga
     6781 tttttttatg tcgatgtaca caaccgccca gctgctggcg gcaaatgagc agaaatttaa
     6841 gtttgatccg ctgtttctgc gtctcttttt ccgtgagagc tatcccttca ctacggagaa
     6901 agtctatctc tcacaaattc cgggactggt aaacatggcg ctgtacgttt cgccgattgt
     6961 ttccggtgag gttatccgtt cccgtggcgg ctccacctct gaatttacgc cgggatatgt
     7021 caaacccaag catgaggtga atccgcagat gaccctgcgt cgcctgccgg atgaagatcc
     7081 gcagaatctg gcggacccgg cttaccgccg ccgtcgcatc atcatgcaga acatgcgtga
     7141 cgaagagctg gccattgctc aggtcgaaga gatgcaggca gtttctgccg tgctcaaggg
     7201 caaatacacc atgaccggtg aagccttcga tccggttgag gtggatatgg gccgcagtgc
     7261 ggcgaacaac atcacacagt ccggcgtcac ggagtggagc aagcgtgaca agtccacgta
     7321 tgacccgacc gacgatatcg aagcctacgc gctgaacgcc agcggtgtgg tgaatatcat
     7381 cgtgtttgac ccgaaaggct gggcgctgtt ccgttccttc aaagccgtca aggagaagct
     7441 ggatacccgt cgcggctctc attccgagct ggagacagcg gtaaaagacc tgggcaaagc
     7501 ggtgtcctat aagggaatgt atggcgatgt ggccatcgtc gtgtattccg gacagtacgt
     7561 ggaaaacggc gtcaaaaaga acttcctgcc ggacaacacg atggtgctgg ggaacactca
     7621 ggctcgcggt ctgcgcacct atggctgcat tcaggatgcg gacgcacagc gcgaaggcat
     7681 taacgcttct gcccgttacc cgaaaaactg ggtgaccacc ggcgatccgg cgcgtgagtt
     7741 caccatgatt cagtcagcac cgctgatgct gctggctgat cctgatgagt tcgtgtccgt
     7801 tcaactggcg taatcatggc ccttcggggc cattttctct ctgtggagga gttcatgacg
     7861 aaagatgaac tgattgcccg tcttcaggag ctgggtgagc aattgaaccg cgatgtcagt
     7921 ttgacgggaa cgaaagaaga actggcgctt cgtgtggcag agctggaaga agagcttgat
     7981 gacacggatg aaactgccgg tcaggacacc cctctcagcc gggaaaatgt gctgaccggg
     8041 catgaaaatg aggtggtatc agcgcagccg gataccgtga ttcaggatac ggctgaactg
     8101 gtcacggtcg tggcactggt gacgctgcat actgatgcac ttcacgccac gcgggataaa
     8161 cctgtggcat ttgtgctgcc gggaacggcg tttcgtgtct ctgccggtgt ggcagccgaa
     8221 atgacagagc gcggcctggc cagaatgcaa taacgggagg cgctgtggct gatttcgata
     8281 acctgttcga tgctgccatt gcccgcgccg atgaaacgat acgcgggtac atgggaacgt
     8341 cagccaccat gacatccggt gagcagtcag gtgcggtgat acgtggtgtt tttgatgacc
     8401 ctgaaaatat cagctatgcc ggacagggcg tgcgcgttga aggctccagc ccgtccctgt
     8461 ttgtccggac tgatgaggtg cggcagctgc ggcgtggaga cacgctgacc atcggtgagg
     8521 aaaatttctg ggtagatcgg gtttcgccgg atgatggcgg aagttgtcat ctctggcttg
     8581 gacggggcgt accgcctgcc gttaaccgtc gccgctgaaa gggggatgta tggccataaa
     8641 aggtcttgag caggccgttg aaaacctcag ccgtatcagc aaaacggcgg tgcccggtgc
     8701 cgccgcaatg gccattaacc gcgttgcttc atccgcgata tcgcagtctg cgtcacaggt
     8761 tgcccgtgag acaaaggtac gccggaaact ggtaaaggaa agggccaggc tgaaaagggc
     8821 cacggtcaaa aatccgcagg ccagaatcaa agttaaccgg ggggatttgc ccgtaatcaa
     8881 gctgggtaat gcgcgggttg tcctgtcccg ccgcaggcgt cataaaaagg ggcagcgttc
     8941 atccctgaaa ggtggcggca gcgtgcttgt ggtgggtaac cgtcgtattc ccggcgcgtt
     9001 tattcagcaa ctgaaaaacg gccggtggca tgttatgcag cgtgtggccg ggaaaaaccg
     9061 ttaccccatt gatgtggtga aaatcccgat ggcggtgccg ctgaccacgg cgtttaaaca
     9121 gaatattgaa cggatacggc gtgaacgtct tccgaaagag ctgggctatg cgctgcagca
     9181 tcaactgaga atggtaataa agcgatgaaa catactgaac tccgtgcagc cgtactggat
     9241 gcactggaga agcatgacac cggggcgacg ctttttgatg gtcgccccgc tgtttttgat
     9301 gaggaagatt ttccggcaat tgccgtttat ctcaccggcg ctgaatacac gggcgaagag
     9361 ctggacagcg atacctggca ggcggagctg catattgaag ttttcctgcc tgctcaggtg
     9421 ccggattcag agctggattc gtggatggag tcccggattt atccggtgat gagcgatatc
     9481 ccggcactgt cagatttgat caccagtatg gtggccagcg gctatgacta ccggcgcgac
     9541 gatgatgcgg gcctgtggag ttcagccgat ctgacttatg tcattatcta tgaaatgtga
     9601 ggacgctatg cctgtaccaa atcctacaat gccggtgaaa ggtgccggga ccaccctgtg
     9661 ggtttataag gggagcggtg acccttatgc gaacccgctt tcagacgttg actggtcgcg
     9721 tctggcaaaa gttaaagacc tgacgcccgg cgaactgacc gctgagtcct atgacgacag
     9781 ctatctcgat gatgaagatg cagactggac tgcgaccggg caggggcaga aatctgccgg
     9841 agataccagc ttcacgctgg cgtggatgcc cggagagcag gggcagcagg cgctgctggc
     9901 gtggtttaat gaaggtgata cccgtgccta taaaatccgc ttcccgaacg gcacggtcga
     9961 tgtgttccgt ggctgggtca gcagtatcgg taaggcggtg acggcgaagg aggtgattac
    10021 ccgcacggtg aaggtcacca atgtgggacg tccgtcgatg gcagaagatc gcagcacggt
    10081 gacggcgacg accggcatga cggtgacacc cgccagtgct tccgtagtga aagggcagag
    10141 caccacgctg accgtggcat tccagccgga tggcgcaacc gacaagagct tccgtgcggt
    10201 gtctgcggat aaaacaaaag ccaccgtgtc ggtcagtggt atgaccatca ccgtgaaagg
    10261 tgttgctgca ggcaaggtca acattccggt cgtatctggt aatggtgagt ttgctgcggt
    10321 tgcagaaatc aacgtcaccg ccagttaatc cggagagtca gcgatgttcc tgaaaaccga
    10381 atcatttgaa cataacggtg tgaccgtcac gctttctgaa ctgtcagccc tgcagcgtat
    10441 tgagcatctc gacctgatga aacggcaggc agaacaggcg gagtcagaca gcaaccggaa
    10501 gtttactgtg gaagacgcca tcagaaccgg cgcttttgtg gttgcgatgt ccctgtggca
    10561 taaccatccg aagaagacgc agatgccgtc catgaatgaa gccgttaaac agattgagca
    10621 ggaagtgctt accacctggc ccacagaggc aatttctcat gctgaaaacg tggtgtaccg
    10681 gctgtccggt atgtatgagt ttgtggtgaa tgatgctcct gaacaggcag aggacgccgg
    10741 gcctgcagag cctgtttctg cgggaaagtg ttcgacggtg agctgagttt tgccctgaaa
    10801 ctggcgcgtg agatggggcg acccgactgg cgcgccatgc ttgccgggat gtcatccacg
    10861 gagtatgccg actggcaccg cttttacagt acccattatt ttcatgatgt tctgctggat
    10921 atgcactttt ccgggctgac gtacaccgtg ctcagcctgt ttttcagcga tccggagatg
    10981 catccgctgg atttcagtct gctgaaccgg cgcgaggctg acgaagagcc tgaagatgat
    11041 gtgctgatgc agaaagcggc agggcttgcc ggaggtgtcc gctttggccc ggacgggaat
    11101 gaagttatcc ccgcttcccc ggatgtggcg gacatgacgg aggatgacgt aatgctgatg
    11161 acagtatcag aagggatcgc aggaggagtc cggtatggct gaaccggtag gcgatctggt
    11221 cgttgatttg agtctggatg cggccagatt tgacgagcag atggccagag tcaggcgtca
    11281 tttttccggt acggaaagtg atgcgaaaaa aacagcggca gtcgttgaac agtcgctgag
    11341 ccgacaggcg ctggctgcac agaaagcggg gatttccgtc gggcagtata aagccgccat
    11401 gcgtatgctg cctgcacagt tcaccgacgt ggccacgcag cttgcagggg ggcagaatcc
    11461 ctggctgatc ctgctgcaac agggtggtca ggttaaggac tccttcggcg ggatgatccc
    11521 catgttcagg gggcttgccg gcgcgatcac cctgccgatg gtgggggcca cctcgctggc
    11581 ggtggcgacc ggtgcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt
    11641 caacaaaacg ctggtccttt ccggtaatca ggcgggactg acggcagatc gtatgctggt
    11701 cctgtccaga gccgggcagg cggcagggct gacgtttaac cagaccagcg agtcactcag
    11761 cgcactggtt aaggcggggg taagcggtga ggctcagatt gcgtccatca gccagagtgt
    11821 ggcgcgtttc tcctatgcat ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa
    11881 gctgaccaca gacccgacgt cggggctgac ggcgatggca cgccagttcc ataacgtgac
    11941 ggcggagcag attgcgtatg ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt
    12001 gcaggcggcg aacgaggccg caacgaaagg gtttgatgac cagacccgcc gcctgaaaga
    12061 gaacatgggc acgctggaga cctgggcaga caggactgca cgtgcattca aatccatgtg
    12121 ggatgcggtg ctggatattg gtcgtcctga taccgcgcag gagatgctga ttaaggcaga
    12181 ggctgcgttt aagaaagcag acgacatctg gaatctgcgc aaggatgatt atttcgttaa
    12241 cgatgaagca cgggcgcgtt actgggatga tcgtgaaaaa aaacgcctgg agcgggatgc
    12301 agcccaaaag agggtagatc aacagcgtca acaggacaaa aatgcgcagc agcagagcga
    12361 taccgaagcg tcacggctga aatataccga agaggcgcag aaggcttacg aacggctgca
    12421 gacgccgctg gagaaatata ccgcccgtca ggaagaactg aacaaggcac tgaaagacgg
    12481 aaaaatcctg caggcagatt acaacacgct gatggcggcg gcgaaaaagg attatgaagc
    12541 gacgctgaaa aagccgaaac agtccggcgt gaaggtgtat gcgggcgatc gtcaggaaga
    12601 cagtgctcat gcggccctgc tgacgcttca ggcagaactc cggacgctgg agaagcatgc
    12661 cggagcgaat gagaaaatca gccagcagcg ccgggatttg tggaaggcag aaagtcagtt
    12721 cgcggtactg gaggaggcgg cacaacgtcg ccagctgtcc gcacaggaga aatccctgct
    12781 ggcgcataaa gacgagacgc tggagtacaa acgccagctg gctgcacttg gtgacaaggt
    12841 tacgtatcag gagcacctga atgcgctggc gcagcaggcg gataaattcg cacagcagca
    12901 acgggcaaaa cgggcagcca ttgatgcgaa aaaccggggg ctgactgacc ggcaggcagc
    12961 gcgggaagcc acggaacagc gcctgaagga acagtatggc gataatcctc tggcgctgaa
    13021 taacgtcatg tcagagcaga aaaagacctg ggcggctgaa gaccagcttc gcgggagctg
    13081 gatggcaggc ctgaagtccg gctggagtga gtgggaagag agcgccacgg acagtatgtc
    13141 acaggttaaa agtgcagcca cgcagacctt tgatggtatt gcacagaata tggcggcgat
    13201 gctgaccggc agtgaacaga actggcgcag cttcacccgc tccgtgctgt ccatgatgac
    13261 agaaattctg cttaagcagg caatggtggg gattgtcggg agtatcggca gcgccattgg
    13321 cggggctgtt ggtggcggcg catccgcgtc aggtggtaca gccattcagg ccgctgcggt
    13381 gaaattccat tttgcaaccg ggggatttac gggaaccggc ggcaaatatg agccagcggg
    13441 gattgttcac cgtggtgaat ttgtcttcac gaaggaggca accagccgga ttggcgtggg
    13501 gaatctctac cggctgatgc gcggctatgc ggaaggtggt tatgtgggcg gtgccggaag
    13561 tccggcgcag atgcggcggg ctgaaggcat taattttaat cagaacaatc acgtggtgat
    13621 tcagaacgac ggtacgaatg gtctgccagg tccacagatg atgaaggccg tgtatgacat
    13681 ggcccgcaag ggtgcccgtg atgaaattca gacacagatg cgtgatggtg gcctgttctc
    13741 cggaggtgga cgatgaaaac cttccgctgg aaagtgaaac ccggtatgga tgtggcttcg
    13801 gccccttctg taagaaaggt gcgctttggt gatggctatt cccagcgagc gcctgccggg
    13861 ctgaatgcca acctgaaaac gtacagcgtg acgctttctg tcccccgtga ggaggccatg
    13921 gtactggagt cgtttctgga agagcacggg ggctggaaag cctttctgtg gacgccgcct
    13981 tatgagtggc ggcagataaa ggtgacctgc gcaaaatggt cgtcgcgggt cagtatgctg
    14041 cgtgttgagt tcagcgcaga gtttgaacag gtggtgaact gatgcaggat atccggcagg
    14101 aaacactgaa tgaatgcacc cgtgcggagc agtcggccag cgtggtgctc tgggaaatcg
    14161 atctgacaga ggtcggtgga gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg
    14221 agccggtcac ctggcagggg cgacagtatc agccgtatcc cattcagggg agtggttttg
    14281 aactgaatgg caaaggcacc agtacgcgcc ccacgctgat ggtttctaac ctgtacggta
    14341 tggtcaccgg gatggcggaa gatctacaga gtctggtcgg cggaacggtg gtccggcgta
    14401 aggtttacgc ccgttttctg gatgcggtga acttcgtcaa cggaaacagt gacgccgatc
    14461 cggagcagga ggtgatcagc cgctggcgca ttgagcagtg cagcgaactg agcgcggtga
    14521 gtgcctcttt tgtactgtcc acgccgacgg aaacagatgg cgctgttttt ccgggacgta
    14581 tcatgctggc caacacctgc acctggacct atcgcggcga tgagtgcggt tatcacggtc
    14641 cggcggtcgc ggatgaatat gaccagccaa cgtccgacat cacgaaggat aaatgcagca
    14701 aatgcctgag tggctgtaag tttcgcaata acgtcggcaa ctttggcggc ttcctttcca
    14761 ttaacaaact ttcgcagtaa atcccatgac agagacagaa tcagcgattc tggcgcacgc
    14821 ccggcgatgt gctccagcgg agtcgtgcgg cttcgtggta agaacgccgg agggggaaag
    14881 atatttcccc tgcgtgaata tctccggtga gccggaggcg tatttccgta tgtcgccgga
    14941 agactggctg caggcagaga tgcagggtga gattgtggca ctggtccaca gccaccccgg
    15001 tggtctgccc tggctgagtg aggccgaccg gcggctgcag gtgcagagtg atttgccgtg
    15061 gtggctggtc tgccgggggg cgattcacaa gttccgctgt gtgccacatc ttaccgggcg
    15121 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt accatctggc
    15181 ggggattgag atgccggatt ttcatcgtga ggatgactgg tggcgtcacg gtcagaatct
    15241 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcag cggcgcagcc
    15301 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
    15361 tggtgacggc gagctgctgc accatattcc tgaacaactg agcaaacgag agaggtatac
    15421 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggcatggc acgcatctgc
    15481 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggtgcc
    15541 gaagccatcc gggcgatggc cacacagctc cctgcgtttc gtcagaagct gagcgacggc
    15601 tggtatcagg tgcgtattgc cgggcaggat gttagcacgt ccggattaac ggcacagtta
    15661 catgaggctc tgcctgacgg cgctgtgatt catattgttc ccagagtcgc cggggccaag
    15721 tcaggtggtg tattccagat tgtcctggga gcagccgcca ttgccggatc attctttacc
    15781 gccggagcca cccttgcagc atggggggct gccattggtg ccggtggtat gaccggcatt
    15841 ctgttttctc tcggtgccag tatggtgctc ggcggtgtgg ctcagatgct ggcaccgaaa
    15901 gccagaactc cccgcacaca gacaacggat aacggtaagc agaacaccta tttctcctca
    15961 ctggataaca tggttgccca gggcaatgtt atgcctgttc tgtatggtga aatgcgcgtg
    16021 gggtcacgtg tggtatctca ggagatcagc acggcagacg aaggggacgg tggtcaggtt
    16081 gtggtgattg gtcgctgatg caaaatgttt tatgtgaaat cgcctccggg cggttttatc
    16141 gtttatggag catgacgaat gggtaaaggc agcagtaagg ggcatacccc gcgcgaagcg
    16201 aaggacaacc tgaagtccac gcagctgctg agtgtgatcg atgccatcag cgaagggccg
    16261 gttgaaggtc cggtggatgg attaaaaagc gtgctgctga acagtacgcc ggtgctggac
    16321 agtgagggga ataccaacat ctccggcgtc acggtggtgt tccgggcagg tgagcaggag
    16381 cagtcaccgc cggagggatt tgaatcctcc ggctccgaga cggtgctggg tacggaagtg
    16441 aaatatgaca cgccgatcac ccggaccatc acgtcggcaa acattgaccg actgcgtttt
    16501 accttcggtg tacaggcact ggtggaaacc acctcaaagg gagacaggaa tccgtctgaa
    16561 gttcgcctgc tggttcagat acagcgtaac ggtggctggg tgacggaaaa agacatcacc
    16621 attaagggca aaaccacctc gcagtatctg gcctcggtgg tggtggataa cctgccgccg
    16681 cgcccgttta atatccggat gcgcagaatg acgccggaca gcaccacaga ccagctgcag
    16741 aacaaaacgc tctggtcgtc atacaccgaa atcatcgatg tgaaacagtg ctacccgaac
    16801 acggcactgg tcggcgtaca ggtggattcg gagcagttcg gcagccagca ggtgagccgt
    16861 aattatcatc ttcgcgggcg cattctgcag gtgccgtcga actataatcc gcagacgcgg
    16921 caatacagcg gtatctggga cggaacgtta aaaccggcat acagcaacaa catggcctgg
    16981 tgtctgtggg atatgctgac ccatccgcgc tatggcatgg ggaaacgtct tggtgcggca
    17041 gatgtggata aatgggcgct gtatgtcatc ggccagtact gtgaccagtc ggtgccggac
    17101 ggctttggca gcacggagcc gcgcatcacc tgtaatgcct ggctgaccac acagcgcaag
    17161 gcgtgggatg tgctcagtga tttctgctcg gcgatgcgct gtatgccggt atggaacggg
    17221 cagacgctga cgttcgtgca ggaccgacag tcggataagg tgtgggccta taaccgcagt
    17281 aatgtggtga tgccggatga tggcgcgccg ttccgctaca gtttcagcgc cctgaaagac
    17341 cgccataatg ccgttgaggt gaactggatt gacccggata acggctggga gacggcgaca
    17401 gagcttgtgg aggatacgca ggccattgtc cgttacggtc gtaacgtcac gaagatggat
    17461 gcctttggct gtaccagccg ggggcaggca caccgcgccg ggctgtggct gattaaaacg
    17521 gaactgctgg agacgcagac cgtggatttc agcgtgggtg ctgaagggct tcgtcatgta
    17581 ccgggcgatg tcattgaaat ctgcgatgat gactatgccg gtatccgcac cggcgggcgc
    17641 gtgctggcgg tgaacagcca gacccggacg ctgacgctcg accgtgaaat cacgctgcca
    17701 tcctccggca ccacgctgat aagcctggtt gacgggcagg ggaatccggt cagcgtggag
    17761 gtccagtccg tcaccgacgg cgtgaaggta aaagtgagcc gtgttcctga cggtgttgct
    17821 gaatacagcg tgtgggggct gaagctgccg acgctgcacc agcgattgtt ccgctgcgtg
    17881 agtatccgtg agaacgacga cggcacgtat gccatcaccg ccgtgcagca tgtgccggaa
    17941 aaagaagcca tcgtggataa cggggcgcac tttgacggcg accagagcgg cacggtgaat
    18001 ggtgtcacgc cgccagcggt gcagcacctg actgccgaag tcaccgcaga cagcggggaa
    18061 tatcaggtgc tggcgcgctg ggacacgccg aaggtggtga agggcgtgag cttcctgctc
    18121 cgtctgaccg taacagcgga tgacggcagt gagcggctgg tcagcacggc ccggacgacg
    18181 gaaaccactt accgcttcac acaactggcg ctggggaact acaggctgac agtccgggca
    18241 gtaaatgcgt gggggcagca gggcgatccg gcgtcggtat cgttccggat tgccgcaccg
    18301 gcagcgctgt cgcggattga gctgacgccg ggctattttc agataaccgc cacgccgcat
    18361 cttgcggttt atgatccgac ggtacagttt gagttctggt tctcggaaaa gcggattgcg
    18421 gatatcaggc aggttgaaac cacagcacgc tatcttggca cggcgctgta ctggatagcc
    18481 gccagtatca atatcaaacc gggccatgat tattactttt atatccgcag tgtgaacacc
    18541 gttggcaaat cggcattcgt ggaggctgtt ggtcagccga gtgatgatgc atccggttat
    18601 ctggattttt tcaaaggcga gatagggaaa acccatctgg ctcaggagct gtggacgcag
    18661 attgataacg gtcagcttgc gcctgacctg gctgaaatca ggacgtccat tacggatgtc
    18721 agcaatgaaa tcacgcagac cgtcaataag aaactggaag accagagtgc ggcaattcag
    18781 cagatacaga aggttcaggt tgatacaaat aataacctga acagcatgtg ggctgtgaag
    18841 ctgcagcaga tgcaggacgg acgcctttat atcgcgggta ttggtgccgg tattgagaac
    18901 acccctgacg gcatgcagag tcaggtgctg ctggcggcgg acaggattgc gatggttaat
    18961 cctgcgaatg gcaacacaaa accgatgttt gttggtcagg gcgatcagat attcatgaac
    19021 gacgtgttcc tgaaacgcct gacggccccc accattacca gcggtggaaa tccatcggca
    19081 ttttccctga ctccggacgg aaagctgacc gctaaaaatg cggatatcag tggcagtgtg
    19141 aatgcgaacg ccgggacgct caacaatgtc acggtaaatg aaaactgtac gattaagggc
    19201 atgctggagg cgactcaggt cagaggtgac ttcgttaaag ctgtatccaa atcatttccg
    19261 aaacaggctg gtacgtgggg taacacggaa acaccaaacg ggacggttac agtcaccata
    19321 agcgatgatc ataactttga ccgccagatt attattccgc ccattatttt taacggtata
    19381 gcgtatgacg atccggggag cggaaataac ccaggaggca cgcgatacac gggttatggt
    19441 tttgaagttc gcaaaaacgg cgtattaatc gcatccagag aaactaaagg tgccattccc
    19501 ggtagttaca gtgcagttat tgatatgcct agtggtggtg gtagcgtcac tctggagttt
    19561 aagattttcc agaaaggcaa tcagggggca ggcaatatca ccgactgtac ggtgattgtg
    19621 accaaaaaag cggcttccgg catcagtatt cgttgaaata tttataaccc caataaatgg
    19681 cgtcaggaat gacgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaagtttg
    19741 tgcagccatt ttgtccgcag ccatttgcct ggccgtatcc ggtgcgcctg catgggcgtc
    19801 tgaacatcag tccacgctga gcgcggggta tcttcatgcc cggaccaacg ttcccggcag
    19861 tgatgatctg aacgggatta acgtgaaata ccgttatgag tttacggata cgctggggct
    19921 ggtgacgtca ttcagctatg caggagacaa gaatcgccag ctgacccgtt acagcgatac
    19981 ccgctggcat gaagattccg tgcgtaaccg ctggttcagc gtgatggcgg ggccgtctgt
    20041 gcgcgtgaat gaatggttca gcgcgtatgc gatggcgggt gtggcttaca gccgtgtgtc
    20101 gactttttcc ggggattata tccgcgtaac tgacaataag gggaaaacgc acgatgtgct
    20161 gaccggaagt gatgacggtc gccacagcaa cacgtctctg gcgcactgac ggtttcatcg
    20221 tgggtgtcgg ttataaattc tgattagcca ggtaacacag tgttatgaca gcccgccggt
    20281 tcaggcgggc ttttttgtgg ggtgaatatg gcagtaaaga tttcaggtgt actgaaagac
    20341 ggcacaggaa aaccggtaca gaactgcaca atccagctga aagcaaaacg taacagcacc
    20401 acggtggtgg tgaacacgct ggcctcagaa aatccggatg aagccgggcg ttacagcatg
    20461 gacgttgagt acggtcagta cagcgttatt ctgttggtgg aaggattccc gccgtcacat
    20521 gccgggacca tcaccgtgta tgaagattcc cgacccggta cgctgaatga ttttctcggt
    20581 gccatgacgg aggatgatgc ccgtccggag gcactgcgcc gttttgagct gatggtggaa
    20641 gaggtggcgc gtaacgcgtc cgcggtggca cagaacacgg cagccgcgaa gaaatcagcc
    20701 agcgatgcca gcacatcagc ccgtgaggcg gcaacccatg cgactgatgc tgcagattca
    20761 gcacgtgcag ccagcacgtc agccggacag gccgcttcgt cggctcagtc agcgtcttcc
    20821 agcgcaggaa cggcatcaac aaaggccact gaagcctcaa aaagtgctgc cgctgcagag
    20881 tcctcaaaaa gcgcggcggc taccagtgcc ggtgcggcga aaacgttaga aacgaatgcg
    20941 gcagcgtcac aacaatcagc agccacttct gcatccaccg cgaccacgaa agcgtcagaa
    21001 gctgccacct cagcccggga tgcgtcggct tcaaaagagg cggcaaaatc atcagaaacg
    21061 aacgcatcat caagcgccag tagcgcagct tcctcggcga cggcggctgc aaattctgcg
    21121 aaggcggcca aaacgtccga gacgaacgcc aggtcttctg aaacggcagc gggacagagt
    21181 gcctcagctg cggcaggctc aaaaacagcg gctgcgtcgt ctgccagcgc cgcgtcaaca
    21241 agtgccgggc aggcctcagc cagtgccacc gccgccggaa aatcggcaga aagcgccgca
    21301 tcatccgctt caacagccac aacgaaggct ggcgaagcca ctgagcaggc cacggcagca
    21361 gcgaggtcag cttccgcagc gaagacatcc gaaacgaacg cgaaagcgtc ggaaacccgt
    21421 gcagaatcct caaaaacggc tgccgcatcg tccgccagtt cggcggcgtc atcggcatca
    21481 tcggcgtctg cttcaaaaga tgaggcgacc agacaggcgt cagcagcgaa gggcagcgcc
    21541 acgacggcat ccacgaaggc gacagaggct gctggtagtg cgacggcagc agctcagagc
    21601 aaaagtacgg cggaatctgc agcaacgcgc gctgagacag cggcaaaacg ggcagaggat
    21661 attgcatccg ccgtggcgct tgaggatgcg agcacgacga aaaaggggat agtacagctc
    21721 agtagtgcga ctaacagcac ttccgagtca ctggcggcaa caccaaaagc cgttaaggcc
    21781 gcgtatgacc tggctaacgg gaaatacacc gcacaggatg caacgacagc acagaaaggg
    21841 ataatccagc taagcagcgc gaccaacagc acgtctgaaa cgctggcggc aacgccaaag
    21901 gcagtaaaag cagccaatga caatgctgag aaacgtctgc agaaagatca gaacggtgcg
    21961 gatatccctg gcaaagacac ctttacgaaa aatattggtg cctgccgtgc cttcggtggg
    22021 tcagtaagca caacaacagg aaactggacg actgcacagt ttatcgagtg gctggattct
    22081 cagggagcat ttaaccatcc atactggatg tgcaagggtt cctggtctta tggcaataat
    22141 aaaatcatta ctgatactgg ctgcggtaat attcatctcg ccggagctgt cattgaagta
    22201 atggggataa agtcagcgat gacgatccgc attaccactc cgaccacatc cagcggtggc
    22261 ggaacaacca acgcccagtt tacctatatt aatcacggaa cagattattc acctggctgg
    22321 cgaagggact ataactccag aaataagcca acggcatcag agatcggggc gctaccgtca
    22381 ggtggaacag cagtatcatc agttaatctg gcttcaaaag gtcgggtagc cgcgctgaca
    22441 gacaatacgc agggggcagc aggtcttgag ttatacgagg tgtataacaa cggatatcca
    22501 acagcgtatg gaaatatcat tcacctgaaa gggatgacag ccgttggcga aggtgagtta
    22561 ctcatcggct ggagtggtac aagcggtgct catgctccgg catttattcg ttcacgacgg
    22621 gatacgaccg acgcaaactg gtcgccgtgg gcgcagcttt acacctcggc tcatcctcct
    22681 gcagagtttt atccagtcgg tgcaccaatc ccgtggccat cagataccgt tccgtctggt
    22741 tatgccctga tgcaggggca gacttttgac aaatctgctt acccgaaact tgcagccgct
    22801 tatccgtcag gcgtgatccc tgatatgcgt ggctggacga ttaagggcaa gcccgccagt
    22861 ggtcgtgccg tattgtctca ggaacaggat ggcattaaat cgcacaccca cagcgccagc
    22921 gcatccagta cggatttggg gacgaaaacc acatcgtcgt ttgattacgg cactaaatcc
    22981 acgaataaca ccggggcgca tacgcacagt ctgagcggct ctacaagttc agctggggcc
    23041 caccaacata gtcagactgg gcccagaacg aatagcggga gtcaacctac tggaatgttt
    23101 ccggcaggga gtactcaagt tagcgggact aatcaggttg gtatcagtgg ctcattaacc
    23161 agtggcacct cccaatgggt aggtaaatca tcttcagaag gaaaccatac acactcactg
    23221 tccggcacag cagcatctgc gggtgcacac gcgcatactg tcggtattgg tgctcatacg
    23281 cactctgttg cgattggttc acatggacac accatcaccg ttaacgctgc tggtaacgcg
    23341 gaaaacaccg tcaaaaacat cgcatttaac tatattgtga ggctcgcata atggcattca
    23401 gaatgagtga acaagcacgg accataaaaa tttataacct gctggccgga actaatgaat
    23461 ttattggtga aggtgacgca tatattccgc ctcatacagg tctgcctgca aacagtaccg
    23521 atattgcacc gccagatatt ccggctggtt tcgtggctgt tttcaacagt gatgagtcat
    23581 cgtggcatct cgttgaagat catcggggta aaacggttta tgacgtggct tccggcgacg
    23641 cgttatttat ttctgaactc ggctcattac cggaaaatgt cacctggtta tccccggaag
    23701 gggagtttca gaagtggaac ggcacagcct gggtgaagga tacggaagca gaaaaactgt
    23761 tccggatccg ggaggcggaa gaaacaaaaa acagcctgat gcaggtagcc agtgagcata
    23821 tagcgccgct tcaggatgct gtggatctgg agatcgcaac ggagaaagaa accttgttgc
    23881 tggaagcttg gaaaaagtat cgggtgttgc tgaaccgtgt tgatacatca actgcaccgg
    23941 atattgaatg gccagtagca cctatagggt aaattcgtaa tgattaccta aatacgttat
    24001 tcttttgtta aaaaagtgat tttgctatag gtagggagta tgttcttgga ttaagaataa
    24061 aaaatcgcca tgaaagagct tcatggcgag tacataaacg tacaaaatgt gatgaaagta
    24121 gagtggcgta agccactata ttaagatatg acaatagcga agaaaagtaa ataacatttt
    24181 atggacgttt aattgttaat tataaaaact agagtgcccg atattgttta tcttgcttta
    24241 aaataatgta ctcaaaccaa atttttgagc taaaagctca aataatataa atgcaataat
    24301 aatgtttaat aagacaatta gcttgctttg tattttcatt tcggaatact gttaagtaac
    24361 tttgatgacc ctaacaatag atcggaattt tcttagagtc aatggtcact gtttttgctt
    24421 gcaaagcaaa tgttttggtg gttataagtc gtcttaatat agtcacgcat gcaacttgtt
    24481 ccatgcggag attaaagatt ggaatataac gcgaagatgg aattaaatta catttgcaat
    24541 aattaattta ttattactca ataattaccg atattattat tgtaaattaa tcattagaat
    24601 gcaaaaagaa atgtaacggc aaaataagag gacgatagct gagaaaaaag cgtgctttcc
    24661 caatctgggg caaatcacgt ctgaatgaca cctgaaaaca ggtggtagcc tcaagaa
//
LOCUS       DAZPJR010000052        24564 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPJR010000052 DAZPJR010000000
VERSION     DAZPJR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.053"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHVB7_001919"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHVB7_001919"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR1913920.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHVB7_001920"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHVB7_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR1913921.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHVB7_001921"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHVB7_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR1913922.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHVB7_001922"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHVB7_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR1913923.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHVB7_001923"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHVB7_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR1913924.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHVB7_001924"
     CDS             5280..5738
                     /locus_tag="ACHVB7_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR1913925.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHVB7_001925"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHVB7_001925"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR1913926.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHVB7_001926"
     tRNA            6790..6874
                     /locus_tag="ACHVB7_001926"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="ACHVB7_001927"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="ACHVB7_001927"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHVB7_001928"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHVB7_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1913927.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHVB7_001929"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHVB7_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1913928.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHVB7_001930"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHVB7_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1913929.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="ACHVB7_001931"
     CDS             complement(10156..13899)
                     /locus_tag="ACHVB7_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR1913930.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHVB7_001932"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHVB7_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR1913931.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="ACHVB7_001933"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="ACHVB7_001933"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR1913932.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="ACHVB7_001934"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="ACHVB7_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR1913933.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHVB7_001935"
                     /gene_synonym="iatA"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHVB7_001935"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR1913934.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="ACHVB7_001936"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="ACHVB7_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR1913935.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHVB7_001937"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHVB7_001937"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR1913936.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHVB7_001938"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHVB7_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR1913937.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="ACHVB7_001939"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="ACHVB7_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR1913938.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="ACHVB7_001940"
     CDS             23294..23401
                     /locus_tag="ACHVB7_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1913939.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            23830..23937
                     /locus_tag="ACHVB7_001941"
     CDS             23830..23937
                     /locus_tag="ACHVB7_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1913940.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            24365..24472
                     /locus_tag="ACHVB7_001942"
     CDS             24365..24472
                     /locus_tag="ACHVB7_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1913941.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         6177 a         6399 c         5956 g         6032 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cgaatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcgacc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc aatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttgtctttat ctcttccaca agtaaatatt ggcaacccga
    23521 gctggaagca tcatccagag cactcatgaa acgactgtaa cagctgttga aaaaccggaa
    23581 tgcactcttc attgcaaaca tttcagggat gaagtgaaga tgaattgagt cgtagtatcc
    23641 acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca
    23701 ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac aacagaacgg
    23761 gggctaatct tgaaccatca acaattcaag aatagccgca aaatgttgtc attacaacaa
    23821 ggcggctata tgacgctcgc gaagtttgcc atgattttct ggcacgacct ggcagcaccg
    23881 atcctggcgg ggattattac cgcagcgatt gtcggctggt ggcgtaaccg gaagtaaggt
    23941 gaaaacgggc gtcaggctgc cgtgatggca atatgcgccc gaaaccggcc gcaggggtaa
    24001 cagcctgcgg ccatttttgt ctttatctcc tccacaagta aatattggca acccgagctg
    24061 gaagcatcat ccagagcact catgaaacga ctgtaacagc tgttgaaaaa ccggaatgca
    24121 ctcttcattg caaacatttc agggatgaag tgaagatgaa ttgagacgta gtatccacat
    24181 caagccggcg ttatccggtg aggcgcaatg ttgcgggggc tttatccctg gtggcattgg
    24241 ttgctggaaa gagaaaaccc ccgcacgttg agaggtttaa cctgacaaca gaacgggggc
    24301 taatcttgaa ccagaacaat tcaagaatag ccgcaaaatg ttgtcattac aacaaggcgg
    24361 ctatatgacg ctcgcgcagt ttgccatgat tttctggcac gacctggcag caccgatcct
    24421 ggcgggaatt attaccgcag cgattgtcgg ctggtggcgt aaccggaagt aaggtgaaaa
    24481 cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac cggccgcagg ggtaacagcc
    24541 tgcggccatt tttgtcttta tctc
//
LOCUS       DAZPJR010000053        23799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPJR010000053 DAZPJR010000000
VERSION     DAZPJR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.054"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(119..1402)
                     /gene="gltA"
                     /locus_tag="ACHVB7_001943"
     CDS             complement(119..1402)
                     /gene="gltA"
                     /locus_tag="ACHVB7_001943"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR1913942.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1608..1715)
                     /locus_tag="ACHVB7_001944"
     CDS             complement(1608..1715)
                     /locus_tag="ACHVB7_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913943.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2096..2500
                     /gene="sdhC"
                     /locus_tag="ACHVB7_001945"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2096..2500
                     /gene="sdhC"
                     /locus_tag="ACHVB7_001945"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1913944.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2494..2841
                     /gene="sdhD"
                     /locus_tag="ACHVB7_001946"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2494..2841
                     /gene="sdhD"
                     /locus_tag="ACHVB7_001946"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR1913945.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2841..4607
                     /gene="sdhA"
                     /locus_tag="ACHVB7_001947"
     CDS             2841..4607
                     /gene="sdhA"
                     /locus_tag="ACHVB7_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR1913946.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4623..5339
                     /gene="sdhB"
                     /locus_tag="ACHVB7_001948"
     CDS             4623..5339
                     /gene="sdhB"
                     /locus_tag="ACHVB7_001948"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR1913947.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5640..8441
                     /gene="sucA"
                     /locus_tag="ACHVB7_001949"
     CDS             5640..8441
                     /gene="sucA"
                     /locus_tag="ACHVB7_001949"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR1913948.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8456..9673
                     /gene="odhB"
                     /locus_tag="ACHVB7_001950"
     CDS             8456..9673
                     /gene="odhB"
                     /locus_tag="ACHVB7_001950"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR1913949.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9767..10933
                     /gene="sucC"
                     /locus_tag="ACHVB7_001951"
     CDS             9767..10933
                     /gene="sucC"
                     /locus_tag="ACHVB7_001951"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR1913950.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10933..11802
                     /gene="sucD"
                     /locus_tag="ACHVB7_001952"
     CDS             10933..11802
                     /gene="sucD"
                     /locus_tag="ACHVB7_001952"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR1913951.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11906..12628)
                     /gene="mngR"
                     /locus_tag="ACHVB7_001953"
     CDS             complement(11906..12628)
                     /gene="mngR"
                     /locus_tag="ACHVB7_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR1913952.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12797..14713
                     /gene="mngA"
                     /locus_tag="ACHVB7_001954"
     CDS             12797..14713
                     /gene="mngA"
                     /locus_tag="ACHVB7_001954"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR1913953.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14731..17364
                     /gene="mngB"
                     /locus_tag="ACHVB7_001955"
     CDS             14731..17364
                     /gene="mngB"
                     /locus_tag="ACHVB7_001955"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR1913954.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            17389..17616
                     /locus_tag="ACHVB7_001956"
     CDS             17389..17616
                     /locus_tag="ACHVB7_001956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1913955.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            18212..19780
                     /gene="cydA"
                     /locus_tag="ACHVB7_001957"
     CDS             18212..19780
                     /gene="cydA"
                     /locus_tag="ACHVB7_001957"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR1913956.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            19796..20935
                     /gene="cydB"
                     /locus_tag="ACHVB7_001958"
     CDS             19796..20935
                     /gene="cydB"
                     /locus_tag="ACHVB7_001958"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1913957.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            20950..21063
                     /gene="cydX"
                     /locus_tag="ACHVB7_001959"
     CDS             20950..21063
                     /gene="cydX"
                     /locus_tag="ACHVB7_001959"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR1913958.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            21063..21356
                     /gene="ybgE"
                     /locus_tag="ACHVB7_001960"
     CDS             21063..21356
                     /gene="ybgE"
                     /locus_tag="ACHVB7_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR1913959.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            21506..21910
                     /gene="ybgC"
                     /locus_tag="ACHVB7_001961"
     CDS             21506..21910
                     /gene="ybgC"
                     /locus_tag="ACHVB7_001961"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1913960.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            21907..22599
                     /gene="tolQ"
                     /locus_tag="ACHVB7_001962"
     CDS             21907..22599
                     /gene="tolQ"
                     /locus_tag="ACHVB7_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR1913961.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            22603..23031
                     /gene="tolR"
                     /locus_tag="ACHVB7_001963"
     CDS             22603..23031
                     /gene="tolR"
                     /locus_tag="ACHVB7_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR1913962.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            23096..>23799
                     /gene="tolA"
                     /locus_tag="ACHVB7_001964"
     CDS             23096..>23799
                     /gene="tolA"
                     /locus_tag="ACHVB7_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR1913963.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAAD"
BASE COUNT         5719 a         5819 c         6453 g         5808 t
ORIGIN      
        1 gaatatatga atgtccgata gtggatttta tttttacaat tttttgtgtt tgggcatata
       61 aaaattaacc cgccatttga acggcgggtt aaaatattta caacttagca atcaaccatt
      121 aacgcttgat atcgctttta aagtcgcgtt tttcatatcc tgtatacagc tgacgcggac
      181 gggcaatctt cataccgtca ctgtgcattt cgctccagtg ggcgatccag ccaacggtac
      241 gtgccattgc gaaaatgacg gtgaacatgg aagacggaat acccatcgct ttcaggatga
      301 taccagagta gaaatcgacg ttcgggtaca gtttcttctc gataaagtac gggtcgttca
      361 gcgcgatgtt ttccagctcc atagccactt ccagcaggtc atctttggtg cccagctctt
      421 tcagaacttc atggcaggtt tcacgcatta cggtggcgcg cgggtcgtaa tttttgtaca
      481 cgcggtgacc gaagcccatc aggcggaaag aatcattttt atctttcgca cgacgaacaa
      541 attccggaat gtgtttaacg gagctaattt cttccagcat tttcagcgcc gcttcgttag
      601 caccaccgtg cgcaggtccc cacagtgaag caatacctgc tgcgatacag gcaaacgggt
      661 tcgcacccga agagccagcg gtacgcacgg tggaggtaga ggcgttctgt tcatggtcag
      721 cgtgcaggat cagaatacgg tccatagcac gttccagaat cggattaact tcatacggtt
      781 cgcacggcgt ggagaacatc atattcagga agttaccggc ataggagaga tcgttgcgcg
      841 gataaacaaa tggctgacca atggaatact tgtaacacat cgcggccatg gtcggcattt
      901 tcgacagcag gcggaacgcg gcaatttcac ggtgacgagg attgttaaca tccagcgagt
      961 cgtgatagaa cgccgccagc gcgccggtaa taccacacat gactgccatt ggatgcgagt
     1021 cgcgacggaa agcatggaac agacgggtaa tctgctcgtg gatcatggta tgacgggtca
     1081 ccgtagtttt aaattcgtca tactgttcct gagtcggttt ttcaccattc agcaggatgt
     1141 aacaaacttc caggtagtta gaatcggtcg ccagctgatc gatcgggaaa ccgcggtgca
     1201 gcaaaatacc ttcatcacca tcaataaaag taattttaga ttcgcaggat gcggttgaag
     1261 tgaagcctgg gtcaaaggtg aacacacctt ttgaaccgag agtacggata tcaataacat
     1321 cttgacccag cgtgcctttc agcacatcca gttcaacagc tgtgtccccg ttgagggtga
     1381 gttttgcttt tgtatcagcc atttaaggtc tccttagcgc cttattgcgt aagactgccg
     1441 gaacttaaat ttgccttcgc acatcaacct ggctttaccc gtttgttatt tggctcgccg
     1501 ctctgtgaaa gaggggaaaa cctgggtaca gagctctggg cgcttgcagg taaaggatcc
     1561 attgatgacg aataaatggc gaatcaagta cttagcaatc cgaattatta aacttgtcta
     1621 ccactaataa ctgtcccgaa tgaattggtc aatacttcca cactgttaca taagttaatc
     1681 ttaggtgaaa taccgacttc ataactttta cgcattatat gcttttcctg gcaatgtttg
     1741 taacaacttt gttgaatgat tgtcaaatta gatgattaaa aattaaataa atgttgttat
     1801 cgtgacctgg atcactgttc aggataaaac ccgacaaact atatgtaggt taattgtaat
     1861 gattttgtga acagcctata ctgccgccag gtctccggaa caccctgcaa tcccgagcca
     1921 cccagcgttg taacgtgtcg ttttcgcatc tggaagcagt gttttgcatg acgcgcagtt
     1981 atagaaagga cgctgtctga cccgcaagca gaccggagga aggaaatccc gacgtctcca
     2041 ggtaacagaa agttaacctc tgtgcccgta gtccccaggg aataataaga acagcatgtg
     2101 ggcgttattc atgataagaa atgtgaaaaa acaaagacct gttaatctgg acctacagac
     2161 catccggttc cccatcacgg cgatagcgtc cattctccat cgcgtttccg gtgtgatcac
     2221 ctttgttgca gtgggcatcc tgctgtggct tctgggtacc agcctctctt cccctgaagg
     2281 tttcgagcaa gcttccgcga ttatgggcag cttcttcgtc aaatttatca tgtggggcat
     2341 ccttaccgct ctggcgtatc acgtcgtcgt aggtattcgc cacatgatga tggattttgg
     2401 ctatctggaa gaaacattcg aagcgggtaa acgctccgcc aaaatctcct ttgttattac
     2461 tgtcgtgctt tcacttctcg caggagtcct cgtatggtaa gcaacgcctc cgcattagga
     2521 cgcaatggcg tacatgattt catcctcgtt cgcgctaccg ctatcgtcct gacgctctac
     2581 atcatttata tggtcggttt tttcgccacc agtggcgaac tgacatatga agtctggatc
     2641 ggtttcttcg cctctgcgtt caccaaagtg ttcaccctgc tggcgctgtt ttctatcttg
     2701 attcatgcct ggatcggcat gtggcaggtg ttgaccgact acgttaaacc gctggcattg
     2761 cgcctgatgc tgcaactggt gattgtcgtt gcactggtgg tttacgtgat ttatggattc
     2821 gttgtggtgt ggggtgtgtg atgaaattgc cagtcagaga atttgatgca gttgtgattg
     2881 gtgccggtgg cgcaggtatg cgcgcggcgc tgcaaatttc ccagagcggc cagacctgtg
     2941 cgctgctctc taaagtcttc ccgacccgtt cccataccgt ttctgcgcaa ggcggcatta
     3001 ccgttgcgct gggtaatacc catgaagata actgggaatg gcatatgtac gacaccgtaa
     3061 aagggtcgga ctatatcggt gaccaggacg cgattgaata tatgtgtaaa accgggccgg
     3121 aagcgattct ggaactcgaa cacatgggcc tgccgttctc gcgtcttgat gatggtcgta
     3181 tctatcaacg tccgtttggc ggtcagtcga aaaacttcgg cggcgagcag gcggcacgca
     3241 ctgcggcggc ggctgaccgt accggtcacg cactgttgca cacgctttat cagcagaacc
     3301 tgaaaaacca caccaccatt ttctccgagt ggtatgcgct ggatctggtg aaaaaccagg
     3361 atggcgcggt ggtgggttgt accgcactgt gcatcgaaac cggtgaagtg gtttatttca
     3421 aagcccgcgc taccgtgctg gcgactggcg gggcagggcg tatttatcag tccaccacta
     3481 acgcccacat taacactggc gacggtgtcg gcatggctat ccgcgcaggc gtaccggtgc
     3541 aggatatgga aatgtggcaa ttccacccga ccggcattgc cggtgcgggc gtactggtca
     3601 ccgaaggttg ccgtggtgaa ggcggttatc tgctgaacaa acatggcgaa cgctttatgg
     3661 agcgttatgc gccgaacgcc aaagacctgg cgggccgtga tgtggtggcg cgttccatca
     3721 tgattgaaat ccgtgaaggt cgcggctgtg atggtccgtg ggggccacac gcgaaactga
     3781 aactcgatca cctgggtaaa gaagttctcg aatcccgtct gccgggtatc ctggagcttt
     3841 cccgtacctt tgctcacgtc gatccggtga aagagccgat tccggttatc ccaacctgtc
     3901 actacatgat gggcggtatt ccgaccaaag tgaccggtca ggcgctgacg gtgaacgaga
     3961 aaggcgaaga tgtggttgtt ccggggctgt ttgccgttgg tgaaatcgct tgtgtatcgg
     4021 tacacggcgc taaccgtctg ggcggcaact cgctgctgga cctggtggtc tttggtcgtg
     4081 cggcaggtct gcatctgcaa gagtctatcg ccgaacaggg cgcattgcgc gatgccagcg
     4141 agtctgatgt tgaagcatct ctggatcgtc tgcaccgctg gaacaataat cgtaacggtg
     4201 aagatccggt ggcgattcgt aaagcgctgc aagaatgtat gcagcataac ttctcggtct
     4261 tccgtgaagg tgatgcgatg gcgaaagggc ttgagcagtt gaaagtgatc cgcgagcgtc
     4321 tgaaaaatgc ccgtctggat gacacttcca gcgagttcaa cacccagcgc gttgagtgct
     4381 tggaactgga taacctgatg gaaacggcgt atgcaacggc tgtttctgcc aacttccgta
     4441 ccgaaagccg tggcgcacat agccgcttcg acttcccgga tcgtgatgat gaaaactggc
     4501 tgtgccactc cctgtatctg ccagagtcgg aatccatgac gcgccgaagc gtcaacatgg
     4561 aaccgaaact gcgcccggca ttcccgccga agattcgtac ttactaatgc ggagacagga
     4621 aaatgagact cgagttttca atttatcgct ataacccgga tgttgatgat gctccgcgta
     4681 tgcaggatta ccccctggaa gcggaagaag gtcgcgacat gatgctgctg gatgcgctta
     4741 tccagctgaa agagaaagat cccagcctgt cgttccgccg ctcctgccgt gaaggtgtgt
     4801 gcggttccga cggtctgaac atgaacggca agaatggtct ggcctgtatt accccgattt
     4861 cggcactcaa ccagccgggc aagaagattg tgattcgccc gctgccaggt ttaccggtga
     4921 tccgcgattt ggtggtagac atgggacaat tctatgcgca atatgagaaa attaagcctt
     4981 acctgttgaa taatggacaa aatccgccag ctcgcgagca tttacagatg ccagagcagc
     5041 gcgaaaaact cgacgggctg tatgaatgta ttctctgcgc atgttgttca acctcttgtc
     5101 cgtctttctg gtggaatccc gataagttta tcggcccggc aggcttgtta gcggcatatc
     5161 gtttcctgat cgatagccgt gataccgaga ctgacagccg cctcgacggt ttgagcgatg
     5221 cattcagcgt attccgctgt cacagcatca tgaactgcgt cagtgtatgt ccgaaggggc
     5281 tgaacccgac gcgcgccatc ggccatatta agtcgatgtt gttgcaacgt aatgcgtaaa
     5341 ccgtaggcct gataagacgc gcaagcgtcg catcaggcaa ccagtgccgg atgcggcgtg
     5401 aacgccttat ccggcctaca agtcattacc cgtaggcctg ataagcgcag cgcatcaggc
     5461 gtaacaaaga aatgcaggaa atctttaaaa actgcccctg acactaagac agtttttaaa
     5521 ggttccttcg cgagccacta catagacaag agctcgcaag tgaaccccgg cacgcacatc
     5581 actgtgcgtg gtagtatcca cggcgaagta agcataaaaa agatgcttaa gggatcacga
     5641 tgcagaacag cgctttgaaa gcctggttgg actcttctta cctctctggc gcaaaccaga
     5701 gctggataga acagctctat gaagacttct taaccgatcc tgactcggtt gacgctaact
     5761 ggcgttcgac gttccagcag ttacctggta cgggagtcaa accggatcaa ttccactctc
     5821 aaacgcgtga atatttccgc cgcctggcga aagacgcttc acgttactct tcaacgatct
     5881 ccgaccctga caccaatgtg aagcaggtta aagtcctgca gctcattaac gcataccgct
     5941 tccgtggtca ccagcatgcg aatctcgatc cgctgggact gtggcagcaa gataaagtgg
     6001 ccgatctgga tccgtctttc cacgatctga ccgaagcaga cttccaggag accttcaacg
     6061 tcggttcatt tgccagcggc aaagaaaaca tgaaactcgg cgagctgctg gaagccctca
     6121 agcaaaccta ctgtggcccg attggtgccg agtatatgca cattaccagc accgaagaaa
     6181 aacgctggat ccaacagcgt atcgagtctg gtcgcgcgac tttcaatagc gaagagaaaa
     6241 aacgcttctt aagcgaactg accgccgctg aaggccttga acgttacctc ggcgcaaaat
     6301 tccctggcgc aaaacgcttc tcgctggaag gcggtgacgc gttaatcccg atgcttaaag
     6361 agatgatccg ccacgctggc aacagcggca cccgcgaagt ggttcttggg atggcgcacc
     6421 gtggtcgtct gaacgtgctg gtgaacgtgc tgggtaaaaa accgcaagac ttgttcgacg
     6481 agtttgccgg taaacataaa gaacacctcg gcacgggtga cgtgaaatac cacatgggct
     6541 tctcgtctga cttccagacc gatggcggcc tggtgcacct ggcgctggcg tttaacccgt
     6601 ctcaccttga gattgtaagc ccggtagtta tcggttctgt tcgtgcccgt ctggacagac
     6661 ttgatgagcc gagcagcaac aaagtgctgc caatcaccat ccacggtgac gccgcagtga
     6721 ccgggcaggg cgtggttcag gaaactctga acatgtcgaa agcgcgtggt tatgaagttg
     6781 gcggtacggt acgtatcgtt atcaacaacc aggttggttt caccacctct aatccactgg
     6841 atgctcgttc tacaccgtac tgtactgata tcggtaagat ggttcaggca ccgattttcc
     6901 acgttaacgc ggatgatccg gaagccgttg cctttgtgac ccgtctggcg ctcgatttcc
     6961 gtaacacctt taaacgtgat gtcttcatcg acctggtgtg ctaccgccgt cacggccaca
     7021 acgaagccga cgagccgagc gcaacccagc cgctgatgta tcagaaaatc aaaaaacatc
     7081 cgacaccgcg caaaatctac gctgacaagc tggagcagga aaaagtggcg acgctggaag
     7141 atgccaccga gatggttaac ctgtaccgcg atgcgctgga tgctggcgat tgcgtagtgg
     7201 cagagtggcg tccgatgaac atgcactctt tcacctggtc gccgtacctc aaccacgaat
     7261 gggacgaaga gtacccgaac aaagttgaga tgaagcgcct gcaggagctg gcgaaacgca
     7321 tcagcacggt gccggaagca gttgaaatgc agtctcgcgt tgccaagatt tatggcgatc
     7381 gccaggcgat ggctgccggt gagaaactgt tcgactgggg cggtgcggaa aacctcgctt
     7441 acgccacgct ggttgacgaa ggcattccgg ttcgcctgtc gggtgaagac tccggtcgcg
     7501 gtaccttctt ccaccgccac gcggtgatcc acaaccagtc taacggttcc acttacacgc
     7561 cgttgcaaca catccataac gggcagggcg cgttccgcgt ctgggactcc gtactgtctg
     7621 aagaagcagt gctggcgttt gaatacggtt atgccaccgc agaaccacgc actctgacca
     7681 tctgggaagc gcagttcggt gacttcgcca acggtgcgca ggtggttatc gaccagttca
     7741 tctcttctgg cgaacagaaa tggggccgga tgtgtggtct ggtgatgttg ctgccgcacg
     7801 gttacgaagg gcaggggccg gagcactcct ccgcgcgtct ggaacgttat ctgcaacttt
     7861 gtgctgagca aaacatgcag gtttgcgttc cgtctacccc ggcacaggtt taccacatgc
     7921 tgcgtcgtca ggcgctgcgc gggatgcgtc gtccgctggt agtgatgtcg ccgaaatccc
     7981 tgctgcgtca tccgctggcg gtttccagcc tcgaagaact ggcgaacggc accttcctgc
     8041 cagccatcgg tgaaatcgac gagcttgatc cgaagggcgt gaagcgcgta gtgatgtgtt
     8101 ctggtaaggt ttattacgac ctgctggaac agcgtcgtaa gaacaatcaa cacgatgtcg
     8161 ccattgtgcg tatcgagcaa ctctacccgt tcccgcataa agcgatgcag gaagtgttgc
     8221 agcagtttgc tcacgtcaag gattttgtct ggtgccagga agagccgctc aaccagggcg
     8281 catggtactg cagccagcat catttccgtg aagtgattcc gtttggggct tctctgcgtt
     8341 atgcaggccg cacggcctcc gcctctccgg cggtagggta tatgtccgtt caccagaaac
     8401 agcaacaaga tctggttaat gacgcgctga acgtcgaata aataaaggat acacaatgag
     8461 tagcgtagat attctggtcc ctgacctgcc tgaatccgta gccgatgcca ccgtcgcaac
     8521 ctggcataaa aaacccggcg acgcagtcgt acgtgatgaa gtgctggtag aaatcgaaac
     8581 tgacaaagtg gtactggaag taccggcatc agcagacggc attctggatg cggttctgga
     8641 agatgaaggt acaacggtaa cgtctcgtca gatccttggt cgcctgcgtg aaggcaacag
     8701 cgccggtaaa gaaaccagcg ccaaatctga agagaaagcg tccactccgg cgcaacgcca
     8761 gcaggcgtct ctggaagagc aaaacaacga tgcgttaagc ccggcgatcc gtcgcctgct
     8821 ggctgaacac aatctcgacg ccagcgccat taaaggcacc ggtgtgggtg gtcgtctgac
     8881 tcgtgaagat gtggaaaaac atctggcgaa agccccggcg aaagagtctg ctccggcagc
     8941 ggctgctccg gcggcgcaac cggctctggc tgcacgtagt gaaaaacgtg tcccgatgac
     9001 tcgcctgcgt aagcgtgtgg cagagcgtct gctggaagcg aaaaactcca ccgccatgct
     9061 gaccacgttc aacgaagtca acatgaagcc gattatggat ctgcgtaagc agtacggtga
     9121 agcgtttgaa aaacgccacg gcatccgtct gggctttatg tccttctacg tgaaagcggt
     9181 ggttgaagcc ctgaaacgtt acccggaagt gaatgcttct atcgacggtg atgacgtggt
     9241 ttaccacaac tatttcgacg tcagcatggc ggtttccacg ccgcgcggcc tggtgacgcc
     9301 ggtactgcgt gatgtcgata ccctcggcat ggcagacatc gagaagaaaa tcaaagagct
     9361 ggcagttaaa ggccgtgacg gcaagctgac ggttgaagat ctgaccggtg gtaacttcac
     9421 catcaccaac ggtggtgtgt tcggttccct gatgtctacg ccgatcatca acccgccgca
     9481 gagcgcaatt ctgggtatgc acgctatcaa agatcgtccg atggcggtga atggtcaggt
     9541 tgagatcctg ccgatgatgt acctggcgct gtcctacgat caccgtctga tcgatggtcg
     9601 cgaatccgtg ggcttcctgg taacgatcaa agagttgctg gaagatccga cgcgtctgct
     9661 gctggacgtg tagtagttta agcttcacct gcactgtaga ccgacgtgga tagggtgtcg
     9721 gtctacggtt taaaagataa cgattactga aggatggaca gaacacatga acttacatga
     9781 atatcaggca aaacaacttt ttgcccgcta tggcttacca gcaccggtgg gttatgcctg
     9841 tactactccg cgcgaagcag aagaagccgc ttcaaaaatc ggtgccggcc cgtgggtagt
     9901 gaaatgtcag gttcacgctg gtggccgcgg taaagcgggc ggtgtgaaag ttgtaaacag
     9961 caaagaagac atccgtgctt ttgcagaaaa ctggctcggc aagcgtctgg taacgtatca
    10021 aacagatgcc aatggccaac cggttaacca gattctggtt gaagcagcga ccgatatcgc
    10081 taaagagctg tatctcggtg ccgttgtaga ccgtagttcc cgtcgtgtgg tctttatggc
    10141 ctccaccgaa ggcggcgtgg aaatcgaaaa agtggcggaa gaaactccgc acctgatcca
    10201 caaagttgcg cttgatccgc tgactggccc gatgccgtat cagggacgcg agctggcgtt
    10261 caaactgggt ctggaaggta aactggttca gcagttcacc aaaatcttca tgggcctggc
    10321 gaccattttc ctggagcgcg acctggcgtt gatcgaaatc aacccgctgg tcatcaccaa
    10381 acagggcgat ctgatttgcc tcgacggcaa actgggcgct gacggcaacg cactgttccg
    10441 ccagcctgat ctgcgcgaaa tgcgtgacca gtcgcaggaa gatccgcgtg aagcacaggc
    10501 tgcacagtgg gaactgaact acgttgcgct ggacggtaac atcggttgta tggttaacgg
    10561 cgcaggtctg gcgatgggta cgatggacat cgttaaactg cacggcggcg aaccggctaa
    10621 cttccttgac gttggcggcg gcgcaaccaa agaacgtgta accgaagcgt tcaaaatcat
    10681 cctctctgac gacaaagtga aagccgttct ggttaacatc ttcggcggta tcgttcgttg
    10741 cgacctgatc gctgacggta tcatcggcgc ggtagcagaa gtgggtgtta acgtaccggt
    10801 cgtggtacgt ctggaaggta acaacgccga actcggcgcg aagaaactgg ctgacagcgg
    10861 cctgaatatt attgcagcaa aaggtctgac ggatgcagct cagcaggttg ttgccgcagt
    10921 ggaggggaaa taatgtccat tttaattgat aaaaacacca aggttatctg ccagggcttt
    10981 actggtagcc aggggacttt ccactcagaa caggccattg catacggcac taaaatggtt
    11041 ggcggcgtaa ccccaggtaa aggcggtact acccacctcg gcctgccggt gttcaacacc
    11101 gtgcgtgaag ctgttgctgc tactggcgct accgcttctg ttatctacgt accagcaccg
    11161 ttctgcaaag actccattct ggaagctatt gatgcaggca tcaaactgat tatcaccatc
    11221 actgaaggca tcccgacgct ggatatgctg actgtgaaag tgaaactgga cgaagccggc
    11281 gttcgtatga tcggcccgaa ctgcccgggc gttatcactc cgggtgaatg caaaatcggt
    11341 atccagccgg gtcacattca caaaccgggt aaagtgggta tcgtttcccg ttccggtaca
    11401 ctgacctatg aagcggttaa acagaccacg gattacggtt tcggtcagtc gacctgtgtt
    11461 ggtatcggcg gtgacccgat cccaggctct aactttatcg acatcctcga aatgttcgaa
    11521 aaagatccgc agaccgaagc gatcgtgatg atcggtgaga tcggcggtag cgctgaagaa
    11581 gaagcggcag cgtacatcaa agagcacgtt accaagccag ttgtaggtta catcgctggt
    11641 gtgactgcgc cgaaaggcaa acgtatgggc cacgcgggtg cgatcattgc tggtgggaaa
    11701 gggactgcgg atgagaaatt cgctgctctg gaagccgcag gcgtgaaaac cgttcgcagc
    11761 ctggcggata tcggtgaagc actgaaaact gttctgaaat aaatatctgt aataagaaat
    11821 agccctcgcc gcttccctct acaggaatgg cgaagggctg tcggtttcga catggttggc
    11881 catcgtatga tggccttttt tgtgcttatc gcgatgattt tcgctgcgct atcagggtaa
    11941 atttatagtc atcggtatta aaagcgttgc ggctatattc aaacacccga ccatcaacta
    12001 aatatccacg cgatactttt tcaagaatcg gctttgtctg gctgatatta agcagacggc
    12061 tcatctcttc ggttggcatc agaggaatga tttcctgttc gctacgatcg ataaccatct
    12121 tcttcacttc ttcaataaag tgatatttcg aattttccat gacctgccag gtgagatccg
    12181 ggaacaacgc aagcggcatc caggtttctt ccagcgccat tggcttttgc ttgcgatagc
    12241 gcacgcgctt cacatgccac acacgatcct gcgcggtgat ttgtagctgt tgctgaagaa
    12301 aatcgtcagc cggaatcact tcaaatatca ggatttcact gtgtgtatcg acgtgacggt
    12361 ccgacagttt ttcatcaaaa ctggttaact gaaaaatatc gtaattgacc cgctcttctt
    12421 tgacgtaagt cccgctgccc tgaatgcttt cgaggatctg ctgctcgact agctggcgca
    12481 aagcctgacg caccgtaacc cggctgacgc caaactctgt ttgtagcgct gattcagtgg
    12541 gtaacgcatc gccaggttta agctcgccac gcgcaatttg ttcacgaatg cgatcggcaa
    12601 tctgccggta taagggcttg tgtcccattt ttagtatctc attaatatga atttagccat
    12661 tatgcccgat aaattcatcc tgtaaataat acaaatacaa tacaaataat ttcaatcaag
    12721 tgaaattgat cacatgatgg tattgtttta tcgggcacac tggcgcgact ataaaaacga
    12781 tcaagtgagg atcatgatga atctgacgac tctgacccac cgcgatgcgt tgtgtctgaa
    12841 tgcgcgcttt accagccgtg aagaggccat ccacgcgttg actcaacgtc ttgctgctct
    12901 ggggaaaatt tccagtactg agcaatttct ggaagaagtg tatcgccgtg aaagccttgg
    12961 cccgaccgcc ttaggtgaag ggttggctgt gccgcatggc aaaactgctg cggtaaaaga
    13021 agcggcgttt gcggtcgcca cactcagcga gccgcttcag tgggaaggcg ttgatggccc
    13081 ggaagcagtt gatttagtgg tgctgctggc gattcccccc aatgaagcgg gtactacgca
    13141 tatgcaactg ctgacagcgc tgaccacgcg ccttgcggat gatgagattc gggcgcgtat
    13201 acagtcggcg acgacgcctg atgagttgct ctcggcgctg gatgacaagg gaggcacgca
    13261 accttctgcc tctttttcca acgcgccaac tatcgtctgc gtaacggcct gtccggcggg
    13321 tattgctcac acctatatgg ctgcggaata tctggaaaaa gccggacgca aactcggcgt
    13381 aaatgtttac gttgaaaaac aaggcgctaa cggcattgaa gggcgtttaa cggcggatca
    13441 actcaatagt gcaaccgcct gtatttttgc ggctgaagtc gccatcaagg agagtgagcg
    13501 ttttaatggc attcccgcgc tttcagtgcc tgttgccgag ccgattcgcc atgcagaagc
    13561 gttgatccaa caagcgctta ccctcaagcg tagcgatgag acgcgtaccg tacagcaaga
    13621 tacgcaaccg gtgaaaagtg tcaaaacgga gctgaaacag gcactgttga gcggaatctc
    13681 ttttgccgta ccgttgattg tcgcgggggg cacggtgctg gcggtcgcgg tattactgtc
    13741 gcaaatcttc gggctacaag atctgtttaa tgaagaaaac tcctggctgt ggatgtaccg
    13801 caagctgggc ggcgggctgc tcggaatttt gatggtaccg gtgctcgcgg cctataccac
    13861 ctattctctg gcagataaac cggcgttagc gccaggcttt gcggctggac ttgccgccaa
    13921 catgatcggc tccgggtttc tcggcgcggt cgttggcgga ttgatagccg gttacttgat
    13981 gcgctgggtg aaaaatcact tgcgtcttag cagtaaattc aatggattcc tgacttttta
    14041 tctctacccg gtgctcggta cgttgggagc gggcagtctg atgctgtttg tggtgggaga
    14101 acctgtcgca tggatcaata actcgcttac cgcctggctg aacggtctgt caggaagtaa
    14161 cgcgctgttg ctgggtgcca ttctcggttt tatgtgttcc tttgaccttg gagggccagt
    14221 gaacaaagcc gcttatgcat tctgcctggg cgcaatggcg aacggcgttt acggcccgta
    14281 tgccattttc gcctccgtca aaatggtttc ggcatttacc gtaaccgctt ccacgatgct
    14341 cgcaccgcgc ctgtttaaag agtttgaaat tgagaccggg aaatccacct ggctgttagg
    14401 gctggcaggt attaccgaag gggcgatccc gatggcgatt gaagatccgc tgcgggttat
    14461 tggttcgttt gtgctgggct ctatggtaac gggcgctatt gtcggtgcga tgaatatcgg
    14521 cctttcgaca cccggtgccg gcattttctc gctcttttta cttcatgata atggcgcggg
    14581 cggtgttatg gcggcaattg gctggtttgg tgcggcattg gtgggggctg caatctcgac
    14641 tgcaattctc ctgatctggc ggcgtcacgc ggttaagcat ggcaactatc tgactgatgg
    14701 cgtaatgcca taaacaaaaa ggaaacgacg atgaaagcgg tatctcgcgt tcacatcacc
    14761 ccgcatatgc attgggatcg ggagtggtat ttcaccaccg aagagtcacg cattctgctg
    14821 gtcaataata tggaagagat cctgtgccga ctggaacagg acaacgaata caaatattac
    14881 gtactcgacg ggcaaacggc gatcctcgaa gattatttcg cggtgaaacc ggaaaacaaa
    14941 gaccgcgtga agaaacaggt agaagccggt aagttgatta tcggcccctg gtatacccag
    15001 accgatacca cgattgtttc tgcggaatcc atcgtccgta atctgatgta cggaatgcgt
    15061 gactgcctcg cgtttggcga gccgatgaaa ataggttatt taccggattc ctttggcatg
    15121 tccgggcaac tgccgcatat ttacaatgga tttggcatta cccgcactat gttctggcgc
    15181 ggatgttcgg agcgccacgg tactgataaa accgagtttt tgtggcaaag cagtgacggt
    15241 agcgaagtga cggcgcaggt gctgccgctg ggctacgcca tcggtaagta cttacctgcc
    15301 gacgaaaacg gattacgtaa acgcctcgac agttattttg acgtgctgga aaaagcgtct
    15361 gtaaccaaag agattttgct gccgaatggt catgaccaga tgccattgca gcaaaatatc
    15421 ttcgaagtga tggataagct acgtgagatc taccctcaac gtaagtttgt gatgagccgc
    15481 tttgaagagg tatttgagaa gatcgaagcg cagcgtgaaa gtctggccac cctgaaaggt
    15541 gaatttatcg atggcaaata tatgcgcgta catcgcacca tcggttccac gcgcatggat
    15601 atcaaaattg cccacgcacg tattgaaaat aagattgtta atctgctgga accgctggca
    15661 acgctggcct ggacgttggg ttttgaatac caccacggct tgctggagaa aatgtggaaa
    15721 gagatcttaa aaaatcatgc ccacgacagt atcggttgtt gttgcagtga caaagttcat
    15781 cgcgaaattg tcgcccgctt cgaactggct gaagacatgg cggataatct gctgcgtttc
    15841 tacatgcgca aaattgccga taacatgcca cagagtgatg ccgacaaact cgtgctgttt
    15901 aacctgatgc cctggccgcg ggaagaagtt attaacacca ctgtgcggct gcgcgccagc
    15961 cagtttaatc tacaagacga tcgcggtcag cctgtaccgt attttattcg ccatgcccgt
    16021 gagatcgatc ctggcctaat cgatcggcaa attgttcatt atggcaatta tgatcccttt
    16081 atggagtttg atattcagat caaccagatt gtcccttcta tgggctatcg cacgctttat
    16141 attgaagcga atcagcctgg caacgtgatt gcggcaaaaa gtgacgctga agggattctg
    16201 gaaaatgctt tctggcagat tgcgctcaat gaggatggtt ctctgcaact ggtagataaa
    16261 gacagcggtg tgcgctatga ccgggtattg caaattgaag aaagctctga tgatggtgat
    16321 gaatatgact attcacccgc aaaagaagag tgggtaatta ccgcagcgaa cgcgaaaccg
    16381 caatgcgata ttattcatga agcctggcag agcagggctg ttatccgcta tgacatggca
    16441 gtgccgctca atttgtcaga acgcagcgcc cggcaatcca ctggcagagt agggggggtg
    16501 ttggttgtca ctcttagtca taacagcagg cgtattgatg tggatatcaa ccttgataac
    16561 caggctgacg atcatcgcct tcgtgtcctg gtccctacac cttttaacac tgacagtgtt
    16621 ctggcagata cgcagtttgg ttcgctaacg cgccccgtga acgacagtgc aatgaacaac
    16681 tggcagcaag aaggctggaa agaagcgccg gttccggtat ggaatatgct caactatgtt
    16741 gccttacagg aagggcgtaa cggcatggct gtctttagcg aagggttacg tgaatttgaa
    16801 gtcatcggtg aagagaagaa aacctttgcc attacgttgc tgcgtggcgt gggcttactg
    16861 ggcaaagaag atctgctttt aaggcctggg cggccttcgg gaattaaaat gccagtcccg
    16921 gactcacaac tacgtggtct gctttcttgt cgcctaagtt tattgagtta taccggtacg
    16981 ccaaccgccg ctggtgtagc tcagcaggcg cgagcatggc tgactccagt acagtgttac
    17041 aacaaaatcc catgggatgc gatgaagctc aacaaagccg gattcaacgt gccggaaagt
    17101 tatagtttgt tgaaaatgcc cccagtggga tgcctgataa gcgcacttaa gaaagctgaa
    17161 gaccgacaag aagtgatttt acggctgttt aatccggctg aatcagcaac ctgtgatgcg
    17221 actgttgctt tcagtcgcga ggtgatttct tgctcagaaa cgatgatgga tgaacacatt
    17281 accaccgagg aaaatcaagg ttcaaatcta tcggggcctt ttttacccgg ccagtcacgg
    17341 acgttcagtt accggcttgc ctgaatagca atcaaaccga agccacatat gcgcggccag
    17401 attgttgaca aagggcgctt tgttcatgcc ggatacggca tgaacgcttt attcggtcta
    17461 caaaagcagg caaattcaat atattgcaga gatgatgtag acactggcaa gcgtagcgca
    17521 tcaggtaatt ttgcgtttat cttcactctc aagccacgta tatgtggctt tatttttaac
    17581 aaaataataa ccctgggtga gttaattata atataattac aagttaatta aatgttaata
    17641 tcggcggggt ggatttatgc ctttattagt aatcctgaaa ctctgcgtcg tattagccag
    17701 tgaccaaaaa aagaattaag gtcaaccgtg ctgtttttgc ttcgtctctt tttatcttta
    17761 aattgccaac cgaaactaat ttcagcctta taactcacac attttaaaca taaatgtcac
    17821 taaagttacc ttattgaaac atgattaaca taatttgtag gaattgatat ttatcaatgt
    17881 ataagtcttg gaaattggca tcaaaaagag ataaattgtt ctcgatcaaa ttggctgaaa
    17941 ggcggtaatt tagctataaa ttgatcaccg tcgaaaaatg caaatttgct tcaacaaaaa
    18001 cctgtttatt gtaaggattt tgcagcgtaa tatatacgtg ggatcaattt gagtttttat
    18061 taacatgttt gcaacctttc tttacgccgt ttttgtgtgc attcacatgg tatgatgaaa
    18121 gtgttcaaac aaatttctat tggggcatgc gtgtgaccct ttctaacggg gttcactctc
    18181 ggagtcttca tgcgatgagc aaggagtcat gatgttagat atagtcgaac tgtcgcgctt
    18241 acagtttgcc ttgaccgcga tgtaccactt cctttttgtg ccactgacgc tcggtatggc
    18301 gttcctgctg gccattatgg aaacggtcta cgtcctctcc ggcaaacaga tttataaaga
    18361 tatgaccaag ttctggggca agttgtttgg tatcaacttc gctctgggtg tggctaccgg
    18421 tctgaccatg gagttccagt tcgggactaa ctggtcttac tattcccact atgtagggga
    18481 tatcttcggt gcgccgctgg caatcgaagg tctgatggcc ttcttcctcg aatccacctt
    18541 tgtaggtctg ttcttcttcg gttgggatcg tctgggtaaa gttcagcata tgtgtgtcac
    18601 ctggctggtg gcgctcggtt caaacctgtc cgcactgtgg attctggttg cgaacggctg
    18661 gatgcaaaac ccaatcgcgt ccgatttcaa ctttgaaact atgcgtatgg agatggtgag
    18721 cttctccgag ctggtgctta acccggttgc tcaggtgaaa ttcgttcaca ctgtagcgtc
    18781 tggttatgtg actggcgcga tgttcatcct cggtatcagc gcatggtata tgctgaaagg
    18841 tcgtgacttc gccttcgcta aacgctcctt tgctatcgct gccagcttcg gtatggctgc
    18901 tgttctgtct gttattgttc tgggtgatga atccggctac gaaatgggcg acgtgcagaa
    18961 aaccaaactg gctgctattg aagccgagtg ggaaacgcaa cctgcgcctg ctgcctttac
    19021 tctgttcggc attcctgatc aggaagagga gacgaacaaa tttgcgattc agatccctta
    19081 cgcactgggc atcattgcaa cgcgttccgt ggataccccg gttatcggcc tgaaagagct
    19141 gatggtgcag catgaagaac gcattcgtaa cgggatgaag gcgtactctc tgctcgaaca
    19201 actgcgttct ggttctaccg accaggcggt tcgtgaccag ttcaatagca tgaagaaaga
    19261 cctcggttac ggtctgctgc tgaaacgcta tacgccaaac gtggctgatg cgactgaagc
    19321 gcagattcaa caggcaacca aagactccat cccgcgtgta gcgccgctgt actttgcgtt
    19381 ccgtatcatg gtggcgtgtg gcttcctgct tctggcaatc atcgcgctct ctttctggag
    19441 tgtcatccgc aaccgcattg gcgagaaaaa atggcttctg cgcgccgcgc tgtacggtat
    19501 tccgctgccg tggattgctg tagaagcggg ctggttcgtg gctgaatatg gccgccaacc
    19561 gtgggctatc ggtgaagtgc tgccgacagc tgtggcgaac tcgtcactga ccgcaggcga
    19621 tctcatcttc tcaatggtgc tgatttgcgg cctgtatacc ctgttcctgg tggcagaatt
    19681 gttcttaatg ttcaagtttg cacgcctcgg cccaagcagc ctgaaaaccg gtcgctatca
    19741 ctttgagcag tcttccacga ctactcagcc ggcacgctaa gacaggagtc gtcaaatgat
    19801 cgattatgaa gtattgcgtt ttatctggtg gctgctggtt ggcgttctgc tgattggttt
    19861 tgcagtcact gacggtttcg acatgggggt gggcatgctc acccgtttcc tcggtcgtaa
    19921 cgacaccgag cgtcgaatta tgattaactc cattgcacca cactgggacg gtaaccaggt
    19981 ttggctgatc accgcgggcg gcgcactctt tgctgcctgg ccgatggtct atgccgctgc
    20041 gttctccggc ttctatgtgg cgatgatcct cgtgctggcg tctttgttct tccgtccggt
    20101 cggttttgac taccgctcca agattgaaga aacccgctgg cgtaacatgt gggactgggg
    20161 catcttcatt ggtagtttcg ttccgccgct ggtaattggt gtagcgttcg gtaacctgtt
    20221 gcagggcgta ccgttcaacg ttgatgaata tctgcgtctg tactacaccg gtaacttctt
    20281 ccagttgctt aacccgttcg gcctgctggc aggcgtggtg agcgtaggga tgatcattac
    20341 tcagggcgca acctatctgc aaatgcgtac cgtgggcgaa ctgcacctgc gtacccgtgc
    20401 aacggctcag gtggctgcgc tggtgacact ggtctgtttc gcactggctg gcgtatgggt
    20461 gatgtacggt atcgatggtt atgtcgtgaa atcgacaatg gaccattacg cagcctctaa
    20521 cccactgaat aaagaagtgg ttcgtgaagc tggcgcatgg ctggttaact tcaacaacac
    20581 gccaattctg tgggctattc cggcactggg tgtggttctt ccgctgctga ccatcctgac
    20641 tgcacgtatg gataaagccg cgtgggcgtt tgtgttctcc tccctgacgc tggcctgcat
    20701 catcctgaca gccggtatcg caatgttccc gtttgtgatg ccgtccagca ccatgatgaa
    20761 cgcaagtctg acaatgtggg atgcaacttc cagccagctg acgcttaacg tcatgacctg
    20821 ggttgcggtg gttctggtac cgatcattct gctctacacc gcttggtgtt actggaaaat
    20881 gttcggtcgt atcaccaaag aagatattga acgtaacacc cactctctgt actaagtaag
    20941 gagctaaaaa tgtggtattt cgcatggatt ctgggaacgc ttcttgcctg ttcgtttggg
    21001 gtaatcaccg cgctggcgct tgaacacgtc gaatcaggca aagccggtca agaagacatc
    21061 tgatgagtaa gattatcgcg accttgtatg cggtaatgga caagcgcccc ctgcgggcgc
    21121 tttccttcgt gatggcgctt ctgttagcag gatgtatgtt ttgggaccca tctcgttttg
    21181 ccgcgaagac cagtgaactg gaaatctggc atggtttatt gctgatgtgg gccgtctgtg
    21241 ctggtgtgat tcacggcgtg ggctttcgtc cgcagaaggt tctttggcaa gggatttttt
    21301 gcccattgct tgccgatatt gttctcattg tcgggctgat tttcttcttc ttttaaatca
    21361 gaattttctt taaaaaatta tgggccgctc caggcccata aatttttacg ctcccttaac
    21421 ttgccctcat tcccaaacct caatcgcgcg cgtatagtag cagcgtttaa aagctctaac
    21481 ttttgttgca ttaccgggat gtaaagtgaa tacaacgctg tttcgatggc cggttcgcgt
    21541 ctactatgaa gataccgatg ccggtggtgt ggtgtaccac gccagttacg tcgcttttta
    21601 tgaaagagca cgcacagaga tgctgcgtca tcatcacttc agtcagcagg cgctgatggc
    21661 tgaacgcgtt gcctttgtgg tacgtaaaat gacggtggaa tattacgcac ctgcgcggct
    21721 cgacgatatg ctcgaaatac agactgaaat aacatcaatg cgtggcacct ctttggtttt
    21781 cacgcaacgt attgtcaacg ccgagaatac tttgctgaat gaagcagagg ttctggttgt
    21841 ttgcgttgac ccactcaaaa tgaagccttg tgcgcttccc aagtctattg tcgcggagtt
    21901 taagcagtga ctgacatgaa tatccttgat ttgttcctga aggctagcct tctggttaaa
    21961 cttatcatgt tgattttgat tggtttttca atcgcatcct gggccattat tatccagcgg
    22021 acccgtattc ttaacgcagc ggcgcgcgaa gccgaagcgt ttgaagataa attctggtct
    22081 ggaatcgaac tctctcgcct ctatcaagag agtcagggga aacgggataa tctgactggt
    22141 tcggaacaaa tattttacag cgggttcaaa gagtttgtgc gcctgcatcg tgccaatagc
    22201 catgcgccgg aagccgtagt ggaaggggcg tcgcgtgcta tgcgtatctc catgaaccgt
    22261 gaacttgaaa atctggaaac gcacattccg ttcctcggta cggttggctc catcagcccg
    22321 tatattggtc tgtttggtac ggtctggggg atcatgcacg cctttatcgc cctcggggcg
    22381 gtaaaacaag caacactgca aatggttgcg cccggtatcg cagaagcgtt gattgcgact
    22441 gcaattggtc tgttcgccgc aattccggcg gtaatggctt ataaccgcct caaccagcgc
    22501 gtaaacaaac tggaactgaa ttacgacaac tttatggaag agtttaccgc gattctgcac
    22561 cgccaggcgt ttaccgttag cgagagcaac aaggggtaag ccatggccag agcgcgtgga
    22621 cgaggtcgtc gcgatctcaa gtccgaaatc aacattgtac cgttgctgga cgtactgctg
    22681 gtgctgttgc tgatctttat ggcgacagcg cccatcatca cccagagcgt ggaggtcgat
    22741 ctgccagacg ctactgaatc acaggcggtg agcagtaacg ataatccgcc agtgattgtt
    22801 gaagtgtctg gtattggtca gtacaccgtg gtggttgaga aagatcgcct ggagcgttta
    22861 ccaccagagc aggtggtggc ggaagtgtcc agccgtttca aggccaaccc gaaaacggtc
    22921 tttctgatcg gtggcgcaaa agatgtgcct tacgatgaaa taattaaagc actgaacttg
    22981 ttacatagtg cgggtgtgaa atcggttggt ttaatgacgc agcctatcta aacatctgcg
    23041 tttcccttgc ttgaaagaga gcgggtaaca ggcgaacagt ttttggaaac cgagagtgtc
    23101 aaaggcaacc gaacaaaacg acaagctcaa gcgggcgata attatttcag cagtgctgca
    23161 tgtcatctta tttgcggcgc tgctctggag ttcgttcgat gagaatatag aagcttcagc
    23221 cggaggcggc ggtggttcgt ccatcgacgc tgtcatggtt gattcaggtg cggtagttga
    23281 gcagtacaaa cgcatgcaaa gccaggaatc aagcgcgaag cgttctgatg agcagcgcaa
    23341 gataaaggaa cagcaggctg ctgaagaact gcgtgagaaa caagcggctg aacaggaacg
    23401 cctgaagcaa cttgagaaag agcggttagc tgctcaggaa cagaaaaagc aggctgaaga
    23461 agccgcaaaa caggccgagt taaagcagaa gcaagcggaa gaggcggcag cgaaagcggc
    23521 ggcagatgct aaagcgaagg ccgaagcgga tgctaaagct gcggaagaag cagcgaagaa
    23581 agcggcggca gacgcgaaga aaaaagcaga agcagaagcc gccaaagccg cagccgaagc
    23641 gcagaaaaaa gccgaggcag ccgctgcggc actgaagaag aaagcggaag cggcagaagc
    23701 tgctgcagct gaagcaagaa agaaagcggc agcagagaaa gctgcagccg acaaaaaagc
    23761 agcagaaaaa gcggctgctg ataaggcagc agctgataa
//
LOCUS       DAZPJR010000054        23563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPJR010000054 DAZPJR010000000
VERSION     DAZPJR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.055"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(506..1303)
                     /locus_tag="ACHVB7_001965"
     CDS             complement(506..1303)
                     /locus_tag="ACHVB7_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1913964.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="ACHVB7_001966"
     CDS             complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="ACHVB7_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR1913965.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="ACHVB7_001967"
     CDS             complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="ACHVB7_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR1913966.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            2546..2674
                     /locus_tag="ACHVB7_001968"
                     /pseudo
     CDS             2546..2674
                     /locus_tag="ACHVB7_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="ACHVB7_001969"
     CDS             complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="ACHVB7_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR1913967.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3229..4887
                     /gene="fliF"
                     /locus_tag="ACHVB7_001970"
     CDS             3229..4887
                     /gene="fliF"
                     /locus_tag="ACHVB7_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR1913968.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4880..5875
                     /gene="fliG"
                     /locus_tag="ACHVB7_001971"
     CDS             4880..5875
                     /gene="fliG"
                     /locus_tag="ACHVB7_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR1913969.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5868..6554
                     /gene="fliH"
                     /locus_tag="ACHVB7_001972"
     CDS             5868..6554
                     /gene="fliH"
                     /locus_tag="ACHVB7_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR1913970.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6554..7927
                     /gene="fliI"
                     /locus_tag="ACHVB7_001973"
     CDS             6554..7927
                     /gene="fliI"
                     /locus_tag="ACHVB7_001973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR1913971.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7946..8389
                     /gene="fliJ"
                     /locus_tag="ACHVB7_001974"
     CDS             7946..8389
                     /gene="fliJ"
                     /locus_tag="ACHVB7_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR1913972.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8386..9513
                     /locus_tag="ACHVB7_001975"
     CDS             8386..9513
                     /locus_tag="ACHVB7_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR1913973.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9618..10082
                     /gene="fliL"
                     /locus_tag="ACHVB7_001976"
     CDS             9618..10082
                     /gene="fliL"
                     /locus_tag="ACHVB7_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR1913974.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            10087..11091
                     /gene="fliM"
                     /locus_tag="ACHVB7_001977"
     CDS             10087..11091
                     /gene="fliM"
                     /locus_tag="ACHVB7_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR1913975.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11088..11501
                     /gene="fliN"
                     /locus_tag="ACHVB7_001978"
     CDS             11088..11501
                     /gene="fliN"
                     /locus_tag="ACHVB7_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR1913976.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11504..11869
                     /gene="fliO"
                     /locus_tag="ACHVB7_001979"
     CDS             11504..11869
                     /gene="fliO"
                     /locus_tag="ACHVB7_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR1913977.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11869..12606
                     /gene="fliP"
                     /locus_tag="ACHVB7_001980"
     CDS             11869..12606
                     /gene="fliP"
                     /locus_tag="ACHVB7_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR1913978.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12616..12885
                     /gene="fliQ"
                     /locus_tag="ACHVB7_001981"
     CDS             12616..12885
                     /gene="fliQ"
                     /locus_tag="ACHVB7_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR1913979.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12894..13679
                     /gene="fliR"
                     /locus_tag="ACHVB7_001982"
     CDS             12894..13679
                     /gene="fliR"
                     /locus_tag="ACHVB7_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR1913980.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13964..14587
                     /gene="rcsA"
                     /locus_tag="ACHVB7_001983"
     CDS             13964..14587
                     /gene="rcsA"
                     /locus_tag="ACHVB7_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR1913981.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14631..14819)
                     /gene="dsrB"
                     /locus_tag="ACHVB7_001984"
     CDS             complement(14631..14819)
                     /gene="dsrB"
                     /locus_tag="ACHVB7_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR1913982.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14982..15209
                     /gene="yodD"
                     /locus_tag="ACHVB7_001985"
     CDS             14982..15209
                     /gene="yodD"
                     /locus_tag="ACHVB7_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR1913983.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15507..16322
                     /locus_tag="ACHVB7_001986"
     CDS             15507..16322
                     /locus_tag="ACHVB7_001986"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR1913984.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16319..18013)
                     /gene="dgcQ"
                     /locus_tag="ACHVB7_001987"
     CDS             complement(16319..18013)
                     /gene="dgcQ"
                     /locus_tag="ACHVB7_001987"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR1913985.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(18184..18366)
                     /locus_tag="ACHVB7_001988"
     CDS             complement(18184..18366)
                     /locus_tag="ACHVB7_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR1913986.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18445..19362)
                     /locus_tag="ACHVB7_001989"
     CDS             complement(18445..19362)
                     /locus_tag="ACHVB7_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR1913987.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19535..20456
                     /gene="yedA"
                     /locus_tag="ACHVB7_001990"
                     /pseudo
     CDS             19535..20456
                     /gene="yedA"
                     /locus_tag="ACHVB7_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(20445..20915)
                     /gene="vsr"
                     /locus_tag="ACHVB7_001991"
     CDS             complement(20445..20915)
                     /gene="vsr"
                     /locus_tag="ACHVB7_001991"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR1913988.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20896..22314)
                     /gene="dcm"
                     /locus_tag="ACHVB7_001992"
     CDS             complement(20896..22314)
                     /gene="dcm"
                     /locus_tag="ACHVB7_001992"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR1913989.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22381..23037)
                     /locus_tag="ACHVB7_001993"
     CDS             complement(22381..23037)
                     /locus_tag="ACHVB7_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR1913990.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(23077..23358)
                     /gene="drpB"
                     /locus_tag="ACHVB7_001994"
     CDS             complement(23077..23358)
                     /gene="drpB"
                     /locus_tag="ACHVB7_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR1913991.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5866 a         6111 c         5875 g         5711 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacctgcttc tgggtaattc gcaatataaa attcatatcc acagggaaat aatattatct
     1141 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
     1201 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
     1261 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
     1321 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
     1381 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
     1441 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
     1501 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
     1561 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
     1621 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
     1681 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
     1741 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
     1801 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
     1861 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
     1921 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
     1981 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
     2041 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     2101 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     2161 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     2221 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     2281 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     2341 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     2401 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     2461 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     2521 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     2581 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     2641 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     2701 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     2761 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     2821 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     2881 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     2941 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     3001 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     3061 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     3121 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     3181 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     3241 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     3301 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     3361 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     3421 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     3481 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     3541 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     3601 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     3661 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     3721 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     3781 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     3841 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     3901 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     3961 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     4021 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     4081 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     4141 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     4201 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     4261 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     4321 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     4381 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     4441 gaagatctga cccgcgaagc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     4501 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     4561 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     4621 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     4681 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     4741 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     4801 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     4861 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     4921 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     4981 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     5041 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     5101 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     5161 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     5221 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     5281 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     5341 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     5401 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     5461 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     5521 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     5581 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     5641 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     5701 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     5761 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     5821 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     5881 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     5941 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     6001 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     6061 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     6121 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     6181 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     6241 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     6301 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     6361 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     6421 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     6481 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     6541 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     6601 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     6661 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     6721 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     6781 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     6841 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     6901 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     6961 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     7021 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     7081 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     7141 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     7201 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     7261 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     7321 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     7381 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     7441 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     7501 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     7561 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     7621 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     7681 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     7741 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     7801 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     7861 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     7921 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     7981 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     8041 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     8101 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     8161 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     8221 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     8281 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     8341 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     8401 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     8461 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     8521 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     8581 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     8641 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     8701 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     8761 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     8821 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     8881 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     8941 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     9001 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     9061 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     9121 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     9181 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     9241 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     9301 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     9361 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     9421 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     9481 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     9541 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     9601 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     9661 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     9721 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     9781 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     9841 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     9901 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     9961 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
    10021 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
    10081 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
    10141 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
    10201 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
    10261 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
    10321 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
    10381 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
    10441 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
    10501 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
    10561 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
    10621 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
    10681 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
    10741 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
    10801 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
    10861 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
    10921 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
    10981 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
    11041 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
    11101 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    11161 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    11221 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    11281 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    11341 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    11401 taatctccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    11461 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    11521 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    11581 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    11641 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    11701 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    11761 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    11821 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    11881 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    11941 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    12001 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    12061 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    12121 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    12181 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    12241 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    12301 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    12361 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    12421 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    12481 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    12541 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    12601 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    12661 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    12721 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    12781 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    12841 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    12901 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    12961 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    13021 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    13081 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    13141 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    13201 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    13261 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    13321 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    13381 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    13441 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    13501 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    13561 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    13621 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    13681 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    13741 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    13801 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    13861 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    13921 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    13981 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    14041 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    14101 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    14161 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    14221 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aaattattga taagttctaa
    14281 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    14341 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    14401 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    14461 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    14521 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    14581 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    14641 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    14701 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    14761 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    14821 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    14881 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    14941 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    15001 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    15061 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    15121 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    15181 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    15241 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    15301 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    15361 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    15421 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    15481 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    15541 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    15601 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    15661 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    15721 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    15781 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    15841 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    15901 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    15961 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    16021 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    16081 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    16141 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    16201 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    16261 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    16321 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    16381 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    16441 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    16501 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    16561 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    16621 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    16681 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    16741 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    16801 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    16861 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    16921 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    16981 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    17041 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    17101 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    17161 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    17221 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    17281 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag
    17341 taaaccagcg taccgcacgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    17401 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    17461 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    17521 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    17581 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    17641 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    17701 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    17761 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    17821 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    17881 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    17941 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    18001 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    18061 gatagcgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    18121 attcattgca ggcattggca caccaggccg gaagtgatcc ggcctggtgt taatgatacc
    18181 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg tttcatgaaa
    18241 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    18301 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    18361 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    18421 tttgcataat agcctgtacg agtattaatg tgcctgaccg cgcattttcg ctacggcttt
    18481 cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg
    18541 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    18601 cggcgcaata ccatgtacca caataccgcc gccgacgagg aacatcgcca gcgtgccgac
    18661 aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttac ctaatgcctg
    18721 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    18781 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    18841 ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga tttcggcaga
    18901 caatataaaa tcggtacgaa tcgccccttt tattttgtcc ttttcaaact tcagcggatc
    18961 ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc ggatcttctt tatgtttacg
    19021 cgcctccagc atatgcagca ctttctctac tccttcaaag cagagaaacg cgccaccaat
    19081 catcaacaga ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    19141 cagaatcact ttattaatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    19201 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    19261 ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagta gtgtggcgat
    19321 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    19381 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    19441 ttttttttac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    19501 atgaaccacc tttttatttc caccgtgagg gattatgcgt ttccgccagt tgttaccgct
    19561 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    19621 tggcgtggaa agctggcctc cgttaatgat ggcaggcgtt cgattcctgg cagccggtat
    19681 tttattgatg gcatttttgc tactgcgcgg acacaagctc cccccgctac gtccgctgct
    19741 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    19801 cgagcatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt
    19861 taccctggtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta
    19921 tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc
    19981 cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg
    20041 gctcgcgcat taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag
    20101 gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg ctcccttccc
    20161 tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccatcaacg
    20221 cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta
    20281 acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg tcgaagattg
    20341 aatggctggc gctcggcgta attgtcttcg cggtggtact ggtcacgttg ggaaaatatc
    20401 tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc gagtaaatga
    20461 atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc
    20521 tcggtaagcg cttcatccgt cagcttctcg cgcccacgta atgcacactc ccagacaatc
    20581 agtacgcgcc agccgagttc ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt
    20641 ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa cagatagcag
    20701 tgatgatgat gccagaagca gccatgggta aatatcacgc agcgatattc atcaacgaca
    20761 aaatccgggc ttccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt
    20821 aacagactgg cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt
    20881 ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc
    20941 caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac
    21001 caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc
    21061 ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg
    21121 acgatgttgc tgattcagcg gatcgtcaaa atctttctca cccgtggcca tatcccagcc
    21181 gcgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac
    21241 gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg
    21301 ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc
    21361 gaccatcggg tccaacagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat
    21421 atcacgcaga gtaaaatcgg ctttcagatt aagatcgcga cgaaaaccca ccagcacgat
    21481 gcgttcacgg tgctgcggca gaaaatgttt accatcgatg attttcggat cgtccggccc
    21541 gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg
    21601 gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc
    21661 cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca
    21721 ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg
    21781 ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc
    21841 cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc ggatatcttc
    21901 attaaaatga tgcgtcgccg gatcgcaata atggttggct ttataagtgc gtaccgcatg
    21961 tttgttccat tcgctggtaa acacgcattg tccgccaatc gattcaaaac cgcgacggat
    22021 gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccga
    22081 tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc
    22141 cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc
    22201 attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc
    22261 ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt
    22321 aaccggccat ctgagatggc cgacaggtta acataattac agccatttag ggccacgcgc
    22381 ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat tctcccccgc
    22441 cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag
    22501 ctgcttgcct cttgcggttt gcatggtttg cggtagcttc agcaacttag tctggaaatg
    22561 atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcaccatc
    22621 aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc
    22681 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    22741 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt tctccgccgg
    22801 aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    22861 acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat aacaggcggt
    22921 taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg
    22981 ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg
    23041 cgctccgttg ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc
    23101 gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa
    23161 taaaaatccg accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa
    23221 tcccgacacc ggggcatcag ggatccggct catcacccag atgtaacgcg ccattgccca
    23281 gacgaaatag ccacaccatg tatagaaagc ccacaaagcc aatttgccgc cagggctgcg
    23341 agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt
    23401 cagacttagt actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa
    23461 gactcaatca ttttttgaac aaatagataa attttgcgca acggcaataa ttactggaaa
    23521 aacagcaata aagatttatt tatatccttg gtaatagttt tat
//
LOCUS       DAZPJR010000055        23237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPJR010000055 DAZPJR010000000
VERSION     DAZPJR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.056"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHVB7_001995"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHVB7_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR1913992.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="ACHVB7_001996"
     CDS             509..1138
                     /locus_tag="ACHVB7_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR1913993.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="ACHVB7_001997"
     CDS             1165..2826
                     /locus_tag="ACHVB7_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR1913994.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="ACHVB7_001998"
     CDS             3065..3124
                     /locus_tag="ACHVB7_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1913995.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3441..3500
                     /gene="ibsE"
                     /locus_tag="ACHVB7_001999"
     CDS             3441..3500
                     /gene="ibsE"
                     /locus_tag="ACHVB7_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR1913996.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="ACHVB7_002000"
     CDS             complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="ACHVB7_002000"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR1913997.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="ACHVB7_002001"
     CDS             complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="ACHVB7_002001"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR1913998.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="ACHVB7_002002"
     CDS             complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="ACHVB7_002002"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR1913999.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9507..10127
                     /locus_tag="ACHVB7_002003"
     CDS             9507..10127
                     /locus_tag="ACHVB7_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR1914000.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10191..11429
                     /gene="cca"
                     /locus_tag="ACHVB7_002004"
     CDS             10191..11429
                     /gene="cca"
                     /locus_tag="ACHVB7_002004"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR1914001.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="ACHVB7_002005"
     CDS             complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="ACHVB7_002005"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1914002.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12504..12872)
                     /gene="folB"
                     /locus_tag="ACHVB7_002006"
     CDS             complement(12504..12872)
                     /gene="folB"
                     /locus_tag="ACHVB7_002006"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR1914003.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12977..13594
                     /gene="plsY"
                     /locus_tag="ACHVB7_002007"
     CDS             12977..13594
                     /gene="plsY"
                     /locus_tag="ACHVB7_002007"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR1914004.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWSVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="ACHVB7_002008"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="ACHVB7_002008"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
     gene            14746..15657
                     /gene="ttdA"
                     /locus_tag="ACHVB7_002009"
     CDS             14746..15657
                     /gene="ttdA"
                     /locus_tag="ACHVB7_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR1914005.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15654..16259
                     /gene="ttdB"
                     /locus_tag="ACHVB7_002010"
     CDS             15654..16259
                     /gene="ttdB"
                     /locus_tag="ACHVB7_002010"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR1914006.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16308..17771
                     /gene="ttdT"
                     /locus_tag="ACHVB7_002011"
     CDS             16308..17771
                     /gene="ttdT"
                     /locus_tag="ACHVB7_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR1914007.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="ACHVB7_002012"
     CDS             complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="ACHVB7_002012"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR1914008.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19064..19279
                     /gene="rpsU"
                     /locus_tag="ACHVB7_002013"
     CDS             19064..19279
                     /gene="rpsU"
                     /locus_tag="ACHVB7_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR1914009.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19390..21135
                     /gene="dnaG"
                     /locus_tag="ACHVB7_002014"
     CDS             19390..21135
                     /gene="dnaG"
                     /locus_tag="ACHVB7_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1914010.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21330..23171
                     /gene="rpoD"
                     /locus_tag="ACHVB7_002015"
     CDS             21330..23171
                     /gene="rpoD"
                     /locus_tag="ACHVB7_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR1914011.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5652 a         6331 c         5907 g         5347 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct
     3361 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
     3421 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
     3481 taagtttccc gacttactaa caactgatca gagggggaag aaatcctccc ttacccttgt
     3541 tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta caaagcgaga
     3601 tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag
     3661 tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt
     3721 ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca
     3781 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
     3841 cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgggaatccc
     3901 ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg tcaccctgtt
     3961 tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag acaccgttgg
     4021 tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt
     4081 cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg
     4141 gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga
     4201 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
     4261 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
     4321 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
     4381 gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
     4441 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
     4501 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt
     4561 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
     4621 caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acgccttcga
     4681 aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg
     4741 tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
     4801 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
     4861 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
     4921 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggag ggaccgtacc
     4981 agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca aactctggca
     5041 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
     5101 tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa
     5161 gcaatccccc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
     5221 cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
     5281 cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg
     5341 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
     5401 agctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca ttttctcgcg
     5461 catttcccgc acttccgttt gcagagttct accttcacgc ggcagcgtca taatctcgcg
     5521 acgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactaccc gcgcacgcac
     5581 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgt
     5641 ggaggtaacc agcattcccg ccgccccgga cgggcgcagc cgcgcatcca cttcgtacaa
     5701 aatgccggaa gaggttcgcg tgctgaacag gtgcataatg cgttgcgcca gacgaagata
     5761 aaactgtcgt ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
     5821 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
     5881 gccgaccact gcaaaaccac gcccttcgcg atcgttgagg tgattcggct taccgtagcg
     5941 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatggctt ccgccagcca
     6001 ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg ctgcggcgat
     6061 gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct cttcgtcatc
     6121 ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg tcgccgtcgg
     6181 ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac gcgccagctg
     6241 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tggttaagcg ccgcagggaa
     6301 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggcggtaat
     6361 gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgcggcat
     6421 cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg
     6481 gaaatcggaa atcagcgtta gcacctgttt gcgatcatcc tcgctaagat gcgccagcac
     6541 tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag
     6601 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
     6661 attgttcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc
     6721 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
     6781 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
     6841 agcatcgttt tcagaaagca gatgcagcgc agcaatggcg ctgagcgttg gcagtaaaga
     6901 gcacgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
     6961 ttcaatttcg cgaataccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
     7021 cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
     7081 gtaacgacgg aaaacaaacg ggcgtagcat cgcacgcaac tcgttagcat agacgccttc
     7141 gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
     7201 ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg
     7261 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
     7321 aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc
     7381 ctgcgtacaa ccgtgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag
     7441 ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc
     7501 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
     7561 aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc
     7621 cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
     7681 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg cagcgtaatg
     7741 ctgccattca tcggcctgcg gcggttggct ttccagttcc gtcaaccact ctcgatgcgc
     7801 aattatgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcacttag
     7861 tggttcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgcctgt actgctgtaa
     7921 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
     7981 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgctcaatt tcaatgcgtt
     8041 gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggca tgcgcgacga
     8101 caggatcata gtaacccgcc agcaacagta ttgagtcaat gtcacgcgtc aggcgtggca
     8161 actggtcacg gtagagatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
     8221 ggtgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctgattttg ccctgcgctt
     8281 tggcatctaa aaatggctgc catgctttgc ttaccaacca ttcggtcaac gccagcttcg
     8341 ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcaatggtcg
     8401 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaataccac
     8461 cgaacagcat cagcgtatga cggaccaggc taatggctgc cagcacctgt tctttcgccg
     8521 catcgttacc gcgtacccac agttcttcat gatactgcca ttgcgctaac gccagctcga
     8581 gcgccgcttc cagcccctgt tctacatcgg cttttgccgc aacatgcaaa atggtggtcg
     8641 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
     8701 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
     8761 gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca aattcccccg
     8821 ctttcacctc cccctgatcg agagcgattt caattcggct gccatcgacc gccaccagcc
     8881 atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg agatcggcag
     8941 gcaattcacc gttcggccag acttccgagg gaaactgcgc caggtcgagc gtcggttcgc
     9001 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
     9061 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac
     9121 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
     9181 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
     9241 actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg
     9301 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
     9361 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
     9421 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
     9481 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
     9541 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
     9601 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg
     9661 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
     9721 acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
     9781 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
     9841 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
     9901 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
     9961 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    10021 aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    10081 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    10141 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    10201 atctggtcgg tggtgctatt cgggatgcat tgttagggct accggtcaaa gacagagatt
    10261 gggtggtagt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    10321 gcgattttcc tgtgtttcta catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg
    10381 aacggaaatc cggttccggt tacaccgatt ttacttgcta tgccgcaccg gatgtcacgc
    10441 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    10501 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    10561 tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc
    10621 gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga
    10681 cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    10741 ccctcaccac ccgcaatcca caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    10801 gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcccctgcc aggtggcatc
    10861 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    10921 cgcaggtcga tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc
    10981 cagaactttg gccgcgtcat catggtcacg gtccggcggg tgtgaaatta gtggaacaac
    11041 tctgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    11101 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aactatcgtc aaattgtttg
    11161 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    11221 ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc
    11281 gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta
    11341 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagttgga
    11401 aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca cgaaccatcc
    11461 cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa
    11521 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    11581 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    11641 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    11701 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg agatatcgcc ggttgtcagg
    11761 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    11821 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    11881 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    11941 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    12001 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    12061 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    12121 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    12181 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    12241 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    12301 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    12361 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    12421 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    12481 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    12541 attacaccaa cattcgccgc ccgcgccatt gcgcctggct tgctgagttt gatgcgcacc
    12601 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    12661 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    12721 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    12781 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    12841 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    12901 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    12961 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    13021 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    13081 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    13141 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    13201 attaggtgtc agccccttct ggttaggctt aattgccatc gccgcctgtc ttggacacat
    13261 ctggtccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    13321 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    13381 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    13441 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    13501 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    13561 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    13621 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    13681 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    13741 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    13801 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    13861 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    13921 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    13981 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    14041 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    14101 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    14161 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    14221 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttacgtga
    14281 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    14341 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    14401 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtctgccca agcgtcgccg
    14461 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    14521 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    14581 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    14641 aattgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    14701 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    14761 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    14821 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    14881 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    14941 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    15001 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    15061 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    15121 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    15181 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    15241 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    15301 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    15361 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    15421 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    15481 caagggctga ctggcaacag ttcagtgatg ggcgtacata tcgaatctgc cgcccgccat
    15541 ccgtcaacca tcggcgttgc tgtctctacc ggctgctggg cgcatcgtcg cggcacactg
    15601 ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    15661 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc gacgatgtga
    15721 tctacctgac cggtacgctg gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac
    15781 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    15841 tgcgcaaaaa cggcgataaa tgggagatgg tttccgttgg cccgacaacc agtatgcgta
    15901 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gttggcaaag
    15961 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    16021 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    16081 ggacagagct cggaatgccg gagtcgctgt gggtctgtcg ggtcaaagag ttcggcccgc
    16141 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    16201 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    16261 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    16321 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    16381 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtgatcg
    16441 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    16501 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
    16561 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    16621 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    16681 ttgcgctgat tctggtgaaa aagatgggac atcgcacgct gtttctcggc tatgcggtga
    16741 tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggga
    16801 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    16861 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    16921 gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg atgaaaagcg
    16981 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    17041 tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta ctgaagtctg
    17101 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    17161 gtgaaaaacg gatgctgggg ctgatagtag gcgcgctggt gctgtggatt ttcggcggtg
    17221 attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg cttctgcgca
    17281 ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    17341 cctcgcttat caccctcgct accggactca acaacaccgg ttttattagc tggtttggca
    17401 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    17461 tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc tccgccctcg
    17521 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    17581 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    17641 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    17701 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    17761 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    17821 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    17881 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaac
    17941 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    18001 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    18061 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    18121 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    18181 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    18241 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    18301 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    18361 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    18421 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    18481 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    18541 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    18601 acagcatcaa tgtcttttgc cgttaagcca gactccttta gcgccgcctg gatcaacggt
    18661 acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc atagtcagcg
    18721 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    18781 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    18841 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    18901 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaggccgcgg
    18961 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    19021 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    19081 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    19141 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    19201 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    19261 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    19321 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    19381 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact
    19441 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    19501 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    19561 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    19621 gacaagctcg agttcgttga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    19681 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat
    19741 cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg
    19801 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    19861 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    19921 aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac
    19981 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    20041 tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    20101 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    20161 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    20221 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    20281 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc
    20341 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    20401 atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    20461 tttgaagcgc ggatggagca ggcgatgccg ctttccgcat ttctgtttaa cagcctgatg
    20521 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    20581 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    20641 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    20701 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    20761 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    20821 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    20881 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    20941 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    21001 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    21061 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    21121 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    21181 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    21241 acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    21301 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    21361 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    21421 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    21481 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    21541 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    21601 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    21661 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    21721 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    21781 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    21841 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    21901 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    21961 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa
    22021 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga
    22081 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    22141 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    22201 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    22261 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    22321 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    22381 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    22441 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    22501 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    22561 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    22621 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    22681 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    22741 tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg
    22801 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    22861 tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc ctcgagctgc
    22921 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc
    22981 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc
    23041 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    23101 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc
    23161 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact agtccctctg
    23221 cacaaacgcc acctttt
//
LOCUS       DAZPJR010000056        22139 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPJR010000056 DAZPJR010000000
VERSION     DAZPJR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.057"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..524)
                     /locus_tag="ACHVB7_002016"
     CDS             complement(<1..524)
                     /locus_tag="ACHVB7_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1914012.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
     gene            820..1596
                     /gene="exuR"
                     /locus_tag="ACHVB7_002017"
     CDS             820..1596
                     /gene="exuR"
                     /locus_tag="ACHVB7_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR1914013.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1836..1919
                     /gene="yqiM"
                     /locus_tag="ACHVB7_002018"
     CDS             1836..1919
                     /gene="yqiM"
                     /locus_tag="ACHVB7_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR1914014.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1941..2603
                     /gene="yqjA"
                     /locus_tag="ACHVB7_002019"
     CDS             1941..2603
                     /gene="yqjA"
                     /locus_tag="ACHVB7_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR1914015.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2607..2990
                     /gene="mzrA"
                     /locus_tag="ACHVB7_002020"
     CDS             2607..2990
                     /gene="mzrA"
                     /locus_tag="ACHVB7_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR1914016.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3137..3505
                     /gene="yqjC"
                     /locus_tag="ACHVB7_002021"
     CDS             3137..3505
                     /gene="yqjC"
                     /locus_tag="ACHVB7_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR1914017.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3543..3848
                     /locus_tag="ACHVB7_002022"
     CDS             3543..3848
                     /locus_tag="ACHVB7_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR1914018.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3851..4255
                     /locus_tag="ACHVB7_002023"
     CDS             3851..4255
                     /locus_tag="ACHVB7_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1914019.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4245..4544
                     /locus_tag="ACHVB7_002024"
     CDS             4245..4544
                     /locus_tag="ACHVB7_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR1914020.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4730..5122
                     /locus_tag="ACHVB7_002025"
     CDS             4730..5122
                     /locus_tag="ACHVB7_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1914021.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5192..6177
                     /gene="yqjG"
                     /locus_tag="ACHVB7_002026"
                     /pseudo
     CDS             5192..6177
                     /gene="yqjG"
                     /locus_tag="ACHVB7_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            6470..6835
                     /locus_tag="ACHVB7_002027"
     CDS             6470..6835
                     /locus_tag="ACHVB7_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1914022.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7077..7433
                     /locus_tag="ACHVB7_002028"
     CDS             7077..7433
                     /locus_tag="ACHVB7_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1914023.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="ACHVB7_002029"
                     /pseudo
     CDS             complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="ACHVB7_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8485..9186
                     /locus_tag="ACHVB7_002030"
     CDS             8485..9186
                     /locus_tag="ACHVB7_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR1914024.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9209..9373
                     /gene="yhaL"
                     /locus_tag="ACHVB7_002031"
     CDS             9209..9373
                     /gene="yhaL"
                     /locus_tag="ACHVB7_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR1914025.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9507..10817)
                     /gene="cyuA"
                     /locus_tag="ACHVB7_002032"
     CDS             complement(9507..10817)
                     /gene="cyuA"
                     /locus_tag="ACHVB7_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR1914026.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10845..12176)
                     /locus_tag="ACHVB7_002033"
     CDS             complement(10845..12176)
                     /locus_tag="ACHVB7_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1914027.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="ACHVB7_002034"
     CDS             complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="ACHVB7_002034"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1914028.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13887..14276)
                     /locus_tag="ACHVB7_002035"
     CDS             complement(13887..14276)
                     /locus_tag="ACHVB7_002035"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR1914029.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="ACHVB7_002036"
     CDS             complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="ACHVB7_002036"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR1914030.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="ACHVB7_002037"
     CDS             complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="ACHVB7_002037"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR1914031.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="ACHVB7_002038"
     CDS             complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="ACHVB7_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR1914032.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="ACHVB7_002039"
     CDS             complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="ACHVB7_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR1914033.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="ACHVB7_002040"
     CDS             complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="ACHVB7_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR1914034.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21546..21764
                     /gene="tdcR"
                     /locus_tag="ACHVB7_002041"
     CDS             21546..21764
                     /gene="tdcR"
                     /locus_tag="ACHVB7_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR1914035.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            22020..>22139
                     /locus_tag="ACHVB7_002042"
     CDS             22020..>22139
                     /locus_tag="ACHVB7_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914036.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEI"
BASE COUNT         5805 a         5650 c         5169 g         5515 t
ORIGIN      
        1 cgttgattcc ctgcattaac taatttttgg gcagcattta cttctaactt agcaataggt
       61 tgctgaggtg gaacactaac aacaatcccc caaattaagt taactgttaa ctgcatagag
      121 agatcttttt tatctgtggc aacgtcatca atactgggaa ccgcttcaaa tttaacgcga
      181 taattttttt ctttttgctc tggttccatt gcaacaaagc gcacagtttt gctactaccg
      241 gctggtaaag cgaatttagg tggcataaca accagatgat ttgcatcacc ggatttaatc
      301 tcaacttcat tttcctgagg cgtcgatgga ttatcgatac ggaatactgt cgccttgatg
      361 tactgaactt cattactttt agatattacg cgaacgttgc cttcaccctg actattaatg
      421 gaaacagcca taggataaac agtcatattt gactgtgcca aaaaaggagc gaacacgaat
      481 aaaagcggcg taagccgtga taagagatta attttcgcca ccatataaat ttgatcttac
      541 aatattgttt ccaaaaagtt ggtgtattag agatgtagat ttattgtaag tcaatataaa
      601 aaagcatttt tagttaatat taaatttaaa ggctgggaat taacttactg aaatttagta
      661 gctttcacaa tgacgatact tattttttag gaatttataa atattcacaa tattaattca
      721 atacattaat tcaatcacca aatttatata tgaccaatgg catacccatg aataaagtgg
      781 tatgataact tcgtccgcat tccactccgg gagacgcata tggaaatcac tgaaccacgc
      841 cgtttgtatc aacaacttgc cgctgacctg aaagagcgca ttgaacaggg cgtctatctg
      901 gtgggtgata aactgcctgc cgaacgcttt attgccgatg aaaagaacgt cagccgaacg
      961 gtagttcgtg aagccatcat catgctggaa gttgaaggct atgtggaggt gcgtaaaggt
     1021 tcaggtattc atgtggtttc caaccagccg cgccatcagc aggcggctga caataatatg
     1081 gaattcgcca attacggtcc gtttgagttg cttcaggctc gccagctcat cgaaagtaat
     1141 attgccgagt tcgcggcaac tcaggtaacg aaacaagaca tcatgaaact gatggccatt
     1201 caggaacagg cgcgcggcga acaatgcttc cgtgattccg agtgggattt acagttccac
     1261 attcaggtag ccctggcgac gcagaactcc gccctggcgg ccatcgttga aaaaatgtgg
     1321 acccagcgta gccataaccc gtactggaaa aaactacacg aacacattga ttcccgtacc
     1381 gttgataact ggtgtgatga ccacgatcaa attctcaagg cgttaattcg caaagatccc
     1441 catgctgcta agctggcaat gtggcagcat ctggaaaaca ccaaaatcat gttatttaac
     1501 gaaaccagcg acgatttcga gttcaatgcc gaccgctatc tattcgccga aaacccggta
     1561 gtgcatctcg atacggccac cagtggcagt aaatgaaact cctgtccgac aggcgctgcg
     1621 acgcgcctgt tcgcttcgtc ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct
     1681 ctcgccaccc tcccctgcat tcagcaaaat cagactccac ggacatgtaa ttttgataac
     1741 gaacaacgtt gacctttgtt acaattagat tcaatttgaa tttatgtttt tgaatgcttt
     1801 cttatctcac gatttaacag ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa
     1861 cataccaaaa cagcaataac attgcggtag tgcatctttt aaaaccagcg tggcgttaac
     1921 cgattcacca ggaataatga atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc
     1981 aggattttga aaccctggcc aatccatcga tgattggcat gttgtatttt gtcttgtttg
     2041 taattttgtt ccttgaaaac ggcttgcttc cagcggcctt tttaccgggc gacagtttac
     2101 tggtattggt cggcgtgttg attgcgaaag gcgcgatggg ttacccgcaa acgattctgc
     2161 tgctgaccgt tgccgccagc ctcggctgct gggtcagcta tattcagggg cgatggctgg
     2221 gcaatacccg caccgtacaa aactggctat ctcatttacc cgcgcattat catcaacgcg
     2281 cacaccatct ttttcataaa cacggtttat cggcgctgtt aattggtcgc tttattgcgt
     2341 ttgtcagaac actgctgccg acgattgccg ggttatcagg gctgaataac gcgcgctttc
     2401 agtttttcaa ctggatgagc ggtctgctgt gggtattgat cctgacaact ctgggttaca
     2461 tgctcggcaa aacgccgata tttttaaagt acgaggacca gctgatgtca tgcctgatgc
     2521 tgctcccggt ggtgctgctg gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa
     2581 agaaatatgg aaatcggggg taagggatgc aaatacctcg catgtcgctt cgccagctag
     2641 cctggtccgg cactgtttta cttctggtcg gcacgctgtt gctggcctgg tccgcggttc
     2701 gccagcaaga gtctacgctg gcgattcgtg ccgttcatca aggcacaacg atgccagacg
     2761 gtttttcgat ctggcatcac cttgacgctc atggcattcc tttcaaaagt atcaccccca
     2821 aaaacgacac tctgttaatt acatttgact ccagcgacca gagcgccgcc gcaaaagcgg
     2881 tcctcgacag aacattgccc catggctaca tcattgcgca gcaggacaat aacagtcagg
     2941 ctatgcagtg gctgacccgg ttacgggata actctcatcg cttcggataa cttcctgtta
     3001 tccgaaacat atcactcact ttggtgattt caccgtaact gtctatgatt aatgaggcgg
     3061 tggttaacac taccgcttcg ttgtctggac aatcgttcct ttgtaatagg tccgaatcac
     3121 aatggaaggt tcaagaatga aataccgcat cgctttagct gtttctctct ttgctcttag
     3181 tgccggtagt tatgccacta ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat
     3241 cagctatgcc gaaaaacacc aaaaccagaa tcgtattgac ggtctgaata aagccctgag
     3301 tgaagtccgg gccaactgtt cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc
     3361 aaagcagaaa gatgaggtgg cggaacgcca gcaagattta gccgaggcga agcaaaaagg
     3421 cgatgccgat aagattgcca aacgcgaacg gaaactggca gaagcgcagg aagagctgaa
     3481 aaagctggaa gcgcgcgact actaactcac aatagtcact acttactcac ctggagaaaa
     3541 ctatgtcgaa agaacacact acggaacatc tgcgtgctga gttgaaatcc ctttccgata
     3601 cgctggaaga ggtgcttagc tcatctggcg agaagtcgaa agaagagttg agtaagattc
     3661 gtagcaaagc ggagcaggca ctgaaacaga gccgttatcg cctgggtgaa accggtgatg
     3721 ccatcgccaa acaaacccgt gtcgcggcgg cgcgtgctga tgagtatgtg cgtgaaaatc
     3781 cgtggaccgg cgtgggaatt ggcgctgcaa tcggtgtagt gctcggcgtt ctgctgtcgc
     3841 gtcgttaatt atggcggaca ctcatcacgc gcaagggccc ggtaaaagcg ttctgggcat
     3901 cgggcagcga attgtttcca tcatggttga aatggtggaa acacgtctgc ggctggcggt
     3961 ggtggagctg gaagaggaaa aagctaatct ctttcaactt ttactgatgc tgggcctgac
     4021 gatgcttttc gctgcatttg gtcttatgag cctgatggtg ctaattattt gggcggttga
     4081 tccgcaatat cgcctgaatg cgatgattgc caccaccgtg gtgttgctgc tactggcact
     4141 gattggcggt atctggacgt tacgtaaatc gcgtaaatcc accttgctgc gccatacacg
     4201 ccatgagctg gcaaacgatc ggcaactgct cgaggaggag tcccgtgagc agtaaagtcg
     4261 aacgtgaacg acgtaaggcg caactgctta gccagatcca gcaacaacgg ctggatcttt
     4321 ccgccagtcg tcgtgaatgg ctggaggcaa caggcgctta cgatcgtcgc tggaatatgc
     4381 tgctaagtct gcgctcctgg gcgctggttg gcagtagcgt gatggcgatc tggacgattc
     4441 gccatcctaa tatgctggtc cgctgggcca gacgcggttt tggcgtatgg agcgcctggc
     4501 gtctggttaa aacgaccctc aagcagcaac agcttcgcgg ttaacttccc tctggccgga
     4561 gcaattccgg ccttatccct caaatttttt gaagattttt gacagttttc cttgctaaca
     4621 atcatcattc accacgttta tgattctctc catcgacagc aacgacgcta ataccgcgcc
     4681 cttgcacaaa aaaacaatca gcagcctgag tggcgcagtg gagagtatga tgaaaaaatt
     4741 agaagatgtt ggtgtactgg tagcgcgcat tttaatgccg attctgttta ttaccgcagg
     4801 ctggggaaaa atcaccgctt actctggtac ccaacaatat atggaagcaa tgggtgtccc
     4861 gggttttatg ctgccgctgg tgattctgct tgagttcggt ggtggtctgg caatcctgtt
     4921 cggtttcctg actcgcacca cagccctgtt tactgcgggc tttacgctgc tgacggcatt
     4981 tttatttcac agcaactttg ctgaaggcgt caactcgctg atgttcatga aaaacctgac
     5041 aatttctggc ggattcctgc tgctggcaat taccggtccg ggcgcgtata gcatcgaccg
     5101 cctgctgaat aaaaagtggt aagcacgcgc tatacttaac gataaaaaac gaggaggaag
     5161 ctcctcgttt ttgctattgg aggagagaaa aatgggtcaa ctgattgacg gcgtctggca
     5221 tgacacctgg tacgatacca aatctaccgg cggtaaattt caacgttcag cttccgcatt
     5281 tcgtaactgg ctcactgccg atggcgcacc tggccccact ggcacaggcg gttttatcgc
     5341 agagaaagat cgttatcatc tctatatttc actcgcctgc ccgtgggcgc accgcacgct
     5401 gatcatgcgc aaactcaaag gactggaacc gtttatttcc gtttccgtag tgaacccgct
     5461 gatgctggaa aacggctgga cctttgatga cagttttccg ggagcaaccg gagatacgct
     5521 ctatcaacat gaatttctgt atcagcttta tctccacgcc gatccacact acagcggacg
     5581 agttactgtt cccgtactgt gggacaaaaa gaaccacacc atcgtcagca acgaatcaac
     5641 agaaatcatc cgcatgttca ataccgcgtt tgatgcgctg ggcgcgaaag cgggtgatta
     5701 ctacccacca gccctgcaaa cgaaaattga cgaacttaac ggctggattt atgacaccgt
     5761 taacaacggc gtgtataaag ctggttttgc caccagccag caagcctacg acgaggcggt
     5821 ggcgaaagtg tttgaatcgc tggcgcgact ggagcagatt ttaggccagc atcgttacct
     5881 gaccggcaac cagctaaccg aagccgatat tcgcctgtgg actacgctgg tgcgttttga
     5941 tccagtgtat gtgacccact tcaagtgtga taagcaccgc atcagcgatt acctgaatct
     6001 gtatggcttc ctgcgcgata tctaccagat gccggggatc gccgaaactg tcaatttcga
     6061 tcacatccgt aatcattact tccgcagcca taagaccatc aaccctacgg ggattatttc
     6121 aattggtccg tggcaggatc tcgatgaacc gcaggacgag atgttcgctt cggttaaaaa
     6181 aaggcacctt acgaagtgcc tctgataatt agctaagtgc atgttgttca tatcggatgc
     6241 ggcgtgaacg ccttatcaaa cctacaaaac atttatattc aatatgttgc aatgaccgcg
     6301 caggccagat aagcgtagcg catctggcga ttttgcgctt gtcatcagtc ttagcgcgcc
     6361 ttcacagcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     6421 cttaatttgc tgctttaaaa acaagtgatt gagcaacatt gaggcaaaaa tggactggta
     6481 tctgaaagta ataaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     6541 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     6601 aggctggcaa cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt
     6661 tttaccgtgg tgggcagttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     6721 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     6781 ggcgggtacg ccaggcgaaa accgctttgg acctgatcca aaactggaac cttaaaacaa
     6841 atctgctcgc cggatgtctg actcatccgg cttttatcat catgttgatg aatgaataat
     6901 ttactaatgt ttatttaaaa tatttcaatg tcgttatata aattacctat aaaaaataac
     6961 catgtattta tcttaaatat caaaaccttt ataacatccg aaaaagaaag atacgaatag
     7021 catttacaca atacccggaa tagactttcg tcacttttaa ttaaagggat gtttttatgc
     7081 agtggtattt atccgtatta aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag
     7141 agtactggat gtttactctg attaacgcca tcgtcggcgc tattatcaat gtcattcaat
     7201 tgattttagg tctggagctt ccatatctgt ctatgcttta tttgctggca accttccttc
     7261 ccgtccttgc gcttgctatc cgtcgcttac acgataccga ccgttcaggt gcctgggcct
     7321 tgctcttttt tgttccgttt attggctggc tcgtgcttct ggtttttttc tgcacagaag
     7381 gtacttctgg cagcaatcgt tacggaaacg atccgaagtt tggttcaaat taatcttaga
     7441 attggggcga tatttcgccc ctttttatta acaataataa atattatttt ccgctaaaaa
     7501 gtttgggaat ttcccgcaga caccaggatt tcgcttcccc catactgtca cgacgccagg
     7561 ccataataat atcgatctca ctggtcgatt ccgggctgac gacacgcaac cgcccttccg
     7621 caatatcttt ttcgaccatc ggatacggca tcgtcgccac gccaagcccc gccagtaatg
     7681 cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg ctgtttgtcc agcagttgta
     7741 cggtcaacac cgggcgctca cgagcggtat ccgctaccgc aattccacga tatttcacgc
     7801 gcgtcacttc agataacggt tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt
     7861 agacgttcat taacgtatag agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca
     7921 tatccggcgc gataacaata tccgcccgcc cctgctccag tcgttcctac gcccccgcca
     7981 gcacttcggt gatgattgcc agttgggtat tggcttttgc cgccagtttg tcgattaacg
     8041 ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat ggtgagatgc gtttcccaac
     8101 cgcgcgcgag ggcttccgca tcggtagtca gtttatctgc ggcttccagc aaaacgcgcc
     8161 ctcgctccag caacatcctc ccgacattgg tgaatttggt gcgatggccc gagcggtcaa
     8221 acagcaccac atccagctct tcttccagtt tttgcatggt gtagctaagt gcggaaggca
     8281 cgcgtcccag ctcatccgcc gccgccgcaa aactgccccg gcgatcgatc gcatccataa
     8341 cccgtagtgc ttccagcgtt aatgcccttt ctttggccat ttcgttctca ttcaggaaat
     8401 ttgaacatac cgggcagaat atctggctaa caatgtagcg tccagcccct taccataaaa
     8461 ggaagtaaag agaggtcaag aattatgatt actacccgaa ctgccaggca gtgtggacaa
     8521 gcagactacg gatggttgca ggcccggtat actttttcct ttggacacta cttcgacccg
     8581 aaattgttag gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc
     8641 gcctttcagc cgcgaaccta tcccaaagtc gatattttaa atgtgattct ggatggagaa
     8701 gcagagtatc gcgacagcga aggcaatcat gttcaggcca gcgccggtga ggcgttgctg
     8761 ctctctaccc agccgggtgt cagctacagc gaacacaatc tcagcaaaga caaaccgtta
     8821 acacgaatgc agctttggct ggacgcctgc ccgcagcgag agaatccgct gattcaaaaa
     8881 ctggcgctta atatgggcaa gcagcaatta atcgcctcgc cagagggtac gatgggaagc
     8941 ctgcaattac gccagcaagt gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg
     9001 aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg
     9061 cttacgcatc atgaagagaa agcggcgctg acctgcggtg atggggcgtt tattcgtgac
     9121 gaggctaaca ttacgctggt tgccgattcc ccactgcgcg ctttgctgat agatttgcct
     9181 gtctagttgt ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa agcgccagcc
     9241 agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga
     9301 gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag ccgaggatga
     9361 agctaccgcg taaagacagg ttgccggatg cggcgaaaac gccttatccg gcctacaaaa
     9421 tccaacaaca ttaacgaatt aaacaacccg taggccggat aaggtgcgcc agcatcgcat
     9481 ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat ctcgataatc
     9541 tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc aatcgactgc
     9601 tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc cagcgccatt
     9661 aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac
     9721 gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc catcgagata
     9781 gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat tgctgcggtc
     9841 gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat tgcgctcaaa
     9901 tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg ttctgctaca
     9961 accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt actcatagcc
    10021 ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat cacaatggat
    10081 gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt cgcgccaata
    10141 tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag cttcgcggaa
    10201 tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag gatctcagcc
    10261 agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc cacacacgcc
    10321 tgctgggtaa acaccacacc attgtgcgtc tcaatacgca caatgttggt atgcccgccg
    10381 acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga gaagaggatt
    10441 tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag tgctttggca
    10501 tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt ggcatttcca
    10561 cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc gggaacggtg
    10621 acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg ttcaaccgga
    10681 ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacat
    10741 cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca taacggattt
    10801 aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga aaatgccagg
    10861 aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga catccctttg
    10921 tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca ccctaccatg
    10981 ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag tacgatagcg
    11041 ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt ttcgttaatc
    11101 ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt tgcttcgcga
    11161 aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag aatgacgctg
    11221 accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat cgccagcgcg
    11281 gaaatattct gctcgtaggc ttttaccgct tcgtcatgcc ccatcgccag cgtgaacgac
    11341 acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat
    11401 gctttatgcc gcgccacttc aatagatttt tcccgcgagc gataagagat caccatcggg
    11461 cttaacgtct ggataaacaa aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt
    11521 ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc
    11581 atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc ggtcgaaatt
    11641 ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat cagtaccaga
    11701 ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg cagataggaa
    11761 gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa
    11821 tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat gacactcggg
    11881 taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg aaacagatac
    11941 attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc
    12001 tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag
    12061 ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca
    12121 cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcgat ttccataatt
    12181 tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa ggcgatatgc
    12241 ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg aggtcagaga
    12301 aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc tactaaatta
    12361 atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg
    12421 tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa tggccagtcc
    12481 tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc
    12541 gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccacgcggc
    12601 gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc
    12661 ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca
    12721 tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca ttgaacaggc
    12781 cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt tcattttata
    12841 cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct cgtttaccgg
    12901 acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt
    12961 cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat
    13021 gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag aaaccagctg
    13081 acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca cgccttcagt
    13141 gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa atcctgcgtc
    13201 aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag atatagacag
    13261 gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacgga
    13321 cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac
    13381 agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga tccgcattcc
    13441 gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc
    13501 aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta actctataaa
    13561 tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgataat
    13621 ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca gatcgaccac
    13681 aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc
    13741 ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt tgaaaatatc
    13801 gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac
    13861 cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc ttcaatttcc
    13921 agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg gtaggtcgcc
    13981 tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc attcagatcg
    14041 gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc aaccacgatc
    14101 gctttgacgt tttcgaggct taaacacgcc tgatcttgca catcagccgg gatctcaccg
    14161 gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa atcaacgccc
    14221 tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat
    14281 cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt gctgttcacg
    14341 ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt
    14401 ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg
    14461 ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt
    14521 ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga aggtgtacga
    14581 gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc
    14641 acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg
    14701 acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag tgatagtcag
    14761 aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt tcttcataaa
    14821 gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg
    14881 atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga ttggtttcac
    14941 gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg cgatgccgca
    15001 tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt cgtagctgta
    15061 cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg ccagccagtc
    15121 catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt ccatcagcgg
    15181 tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt taattgcgta
    15241 cagcagtgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct taccaatcac
    15301 cattgggctg acgcaacaag caattgcgta atcgtcgctg ttgaagtcag tacgcatcag
    15361 atcgtcgttt tcatactgca aggaagaggt gacgatcgac acctgcgcgg catatttttt
    15421 gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg gtgccggccc
    15481 catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc
    15541 cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga acagcgaatc
    15601 aaattccggc gtacgcaaga agcgcaccat acggatcttc ataatgaagt gatcgatcag
    15661 ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc
    15721 gaggaacgac gccgtgcgcc ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc
    15781 cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat
    15841 atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac gatgctctgc
    15901 cagctcctca cgcagacgga tggtggcttc cagatcttcg cctttttcca gacgggactg
    15961 gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat acagcgctac
    16021 gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac cggtcagcac
    16081 gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg
    16141 ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac ggccataggc
    16201 gtggaatgaa cttttaatca tattaatgcc accgaacgga tgcagcgcgc gctttaatgg
    16261 cgcatccgtt tgcagaccaa caattttttc cagcggctgg ttaatatatc ccgcatcatg
    16321 agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc
    16381 gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg tcgcttcggc
    16441 gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat cgcggacatt
    16501 aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt acagcttatc
    16561 gctggtatca atatctatct tcatgaaaaa taatctctct acaatacttc aactaaatta
    16621 tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag caatcatttt
    16681 ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg aaacaattcg
    16741 ctctccaaaa gagttagagc gattattcat ttctgtatca atcactacgc ctaatacagc
    16801 caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa
    16861 tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac gggcaattcg
    16921 gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc
    16981 cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact ctttattcac
    17041 tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg acatcgcacc
    17101 aaagtcgaca tcgccactgc gggttcccat catcaagcct tccagcggcg tcattcccat
    17161 tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg
    17221 cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgtg cgcgctggga
    17281 aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata
    17341 tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc
    17401 aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa
    17461 attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc
    17521 atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat
    17581 taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc
    17641 accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc
    17701 attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatc
    17761 gagcacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc
    17821 attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga
    17881 tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca
    17941 gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat gcgataatcg
    18001 gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg
    18061 tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac actttagttt
    18121 tgtcgccttt ataaccaaac ttcaggacca ggccattcag accttccagc gttcccagat
    18181 agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca
    18241 gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta gccagataag
    18301 aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac agcgtaaata
    18361 ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga
    18421 tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt
    18481 tagaaaccac gaaggaagag acgattggcg agaaattaaa ggagaaaacc atgatggaaa
    18541 tccccagcca gacggtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt
    18601 caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa atcagcacca
    18661 ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa
    18721 accagatgac gaaagccatc agcagcagca ggaacagcgc cacaaagccg cgattcagcg
    18781 gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat
    18841 aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag
    18901 ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa
    18961 gacatagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc aataccaaca
    19021 tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg
    19081 cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg
    19141 attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa
    19201 ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc
    19261 cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata
    19321 atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg
    19381 caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac
    19441 gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc
    19501 cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt
    19561 tttcagactg tacgccaata acacgaatgg tcgggttaat agatttaatt gccaccgcaa
    19621 taccagcaat taaaccgcca ccaccaattg gcacaatcac gttatcgaca tcatagagat
    19681 cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag
    19741 gtgggataaa aatacggcct tccatttcga caatttcgct cactttagcg atagtgtcgt
    19801 tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc gctactttgg
    19861 attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc gcgcaggaga
    19921 gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg cgtttttccg
    19981 catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa cccgtacgct
    20041 gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc
    20101 gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca ataatatcat
    20161 caatagcaat cggcagatcg tatgtaatat gcattataaa acctcttcgc ctgaattaaa
    20221 tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt atgtttaaat
    20281 aaaaacaaat aactaaccaa cttctattaa ttgccttcgt ctacacccat tataagatga
    20341 atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga
    20401 ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg
    20461 attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat cagcattgag
    20521 aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc
    20581 atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt gtggcaacac
    20641 ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact
    20701 ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat
    20761 ttctgcactt aatgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa
    20821 cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt
    20881 gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc
    20941 caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt
    21001 aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt
    21061 tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga ttttactgac
    21121 ggccggttga gttaacccta attcttttgc agccgagccg atagagccac ttctaatgac
    21181 ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta
    21241 cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg
    21301 accactcaca aattaacttt caatactttc cagggtatcg tcattacaat taaatacctt
    21361 taaatatcaa caagttaaag cataaaaatc agcataaacc ctgatttttg tcaaaaaaaa
    21421 tgacggggat aaccatattt tatggcgata acatcattcg ttatgtggtt aatacaaaaa
    21481 aggggctgag accatatttc aagcaattac cggataatta ccaggcaaaa tttgagttaa
    21541 atttaatgag caagttctca aattttatta taaataaacc attttcagcg ataaatactg
    21601 cggcacgtca cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt
    21661 ttaaaatttc gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct
    21721 cttctgacag gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccctcat
    21781 aggcgacgaa tagcattttg tgttgaggat cacaaaacga ataattgctg atcgccgcga
    21841 taaggtcaga caaagacaac aagggaaatt ttcacagagc ttttgatcgt cgtaggccac
    21901 agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgaaag
    21961 aaccctgcga ggaaaataag catctattat tgtttgtcat gacgacaagg caggcatcaa
    22021 tgaaaggctt cccaattgca catatttttc acccttcaat cccgccaatg cacgcagtgg
    22081 ttaacaatca caatagaaat attgattatt ggacggtaaa aagaaagttt gcagaaatt
//
LOCUS       DAZPJR010000057        22131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPJR010000057 DAZPJR010000000
VERSION     DAZPJR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.058"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..164)
                     /locus_tag="ACHVB7_002043"
     CDS             complement(<1..164)
                     /locus_tag="ACHVB7_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFR1914037.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGF"
     gene            complement(186..497)
                     /gene="traL"
                     /locus_tag="ACHVB7_002044"
     CDS             complement(186..497)
                     /gene="traL"
                     /locus_tag="ACHVB7_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR1914038.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(512..871)
                     /gene="traA"
                     /locus_tag="ACHVB7_002045"
     CDS             complement(512..871)
                     /gene="traA"
                     /locus_tag="ACHVB7_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR1914039.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(905..1132)
                     /gene="traY"
                     /locus_tag="ACHVB7_002046"
     CDS             complement(905..1132)
                     /gene="traY"
                     /locus_tag="ACHVB7_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFR1914040.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(1252..1899)
                     /locus_tag="ACHVB7_002047"
     CDS             complement(1252..1899)
                     /locus_tag="ACHVB7_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFR1914041.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFGCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(2091..2474)
                     /gene="traM"
                     /locus_tag="ACHVB7_002048"
     CDS             complement(2091..2474)
                     /gene="traM"
                     /locus_tag="ACHVB7_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR1914042.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            2795..3397
                     /locus_tag="ACHVB7_002049"
     CDS             2795..3397
                     /locus_tag="ACHVB7_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR1914043.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(3694..4515)
                     /locus_tag="ACHVB7_002050"
     CDS             complement(3694..4515)
                     /locus_tag="ACHVB7_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR1914044.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(4626..4922)
                     /locus_tag="ACHVB7_002051"
     CDS             complement(4626..4922)
                     /locus_tag="ACHVB7_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914045.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            5222..5518
                     /locus_tag="ACHVB7_002052"
                     /pseudo
     CDS             5222..5518
                     /locus_tag="ACHVB7_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5837..5962)
                     /locus_tag="ACHVB7_002053"
     CDS             complement(5837..5962)
                     /locus_tag="ACHVB7_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFR1914046.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(5904..6053)
                     /gene="mok"
                     /locus_tag="ACHVB7_002054"
                     /pseudo
     CDS             complement(5904..6053)
                     /gene="mok"
                     /locus_tag="ACHVB7_002054"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            complement(6232..6994)
                     /locus_tag="ACHVB7_002055"
                     /pseudo
     CDS             complement(6232..6994)
                     /locus_tag="ACHVB7_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(6991..7425)
                     /gene="psiB"
                     /locus_tag="ACHVB7_002056"
     CDS             complement(6991..7425)
                     /gene="psiB"
                     /locus_tag="ACHVB7_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR1914047.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(7480..9438)
                     /locus_tag="ACHVB7_002057"
     CDS             complement(7480..9438)
                     /locus_tag="ACHVB7_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR1914048.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(9502..9735)
                     /locus_tag="ACHVB7_002058"
     CDS             complement(9502..9735)
                     /locus_tag="ACHVB7_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR1914049.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(9791..10312)
                     /locus_tag="ACHVB7_002059"
     CDS             complement(9791..10312)
                     /locus_tag="ACHVB7_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR1914050.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            10611..11048
                     /locus_tag="ACHVB7_002060"
                     /pseudo
     CDS             10611..11048
                     /locus_tag="ACHVB7_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11134..11697)
                     /locus_tag="ACHVB7_002061"
     CDS             complement(11134..11697)
                     /locus_tag="ACHVB7_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR1914051.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(11744..13105)
                     /locus_tag="ACHVB7_002062"
     CDS             complement(11744..13105)
                     /locus_tag="ACHVB7_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFR1914052.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(13157..13387)
                     /locus_tag="ACHVB7_002063"
     CDS             complement(13157..13387)
                     /locus_tag="ACHVB7_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914053.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(13611..13730)
                     /locus_tag="ACHVB7_002064"
                     /pseudo
     CDS             complement(13611..13730)
                     /locus_tag="ACHVB7_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332787.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(14425..14616)
                     /locus_tag="ACHVB7_002065"
     CDS             complement(14425..14616)
                     /locus_tag="ACHVB7_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914054.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(14613..15035)
                     /locus_tag="ACHVB7_002066"
     CDS             complement(14613..15035)
                     /locus_tag="ACHVB7_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR1914055.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(15082..15507)
                     /locus_tag="ACHVB7_002067"
     CDS             complement(15082..15507)
                     /locus_tag="ACHVB7_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR1914056.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(15925..16701)
                     /locus_tag="ACHVB7_002068"
     CDS             complement(15925..16701)
                     /locus_tag="ACHVB7_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914057.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(16752..17186)
                     /locus_tag="ACHVB7_002069"
     CDS             complement(16752..17186)
                     /locus_tag="ACHVB7_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR1914058.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(17200..17421)
                     /locus_tag="ACHVB7_002070"
     CDS             complement(17200..17421)
                     /locus_tag="ACHVB7_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914059.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(17422..18105)
                     /locus_tag="ACHVB7_002071"
     CDS             complement(17422..18105)
                     /locus_tag="ACHVB7_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR1914060.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(18182..18475)
                     /locus_tag="ACHVB7_002072"
     CDS             complement(18182..18475)
                     /locus_tag="ACHVB7_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914061.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            18622..19584
                     /gene="parM"
                     /locus_tag="ACHVB7_002073"
                     /gene_synonym="stbA"
     CDS             18622..19584
                     /gene="parM"
                     /locus_tag="ACHVB7_002073"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR1914062.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            19587..19937
                     /locus_tag="ACHVB7_002074"
     CDS             19587..19937
                     /locus_tag="ACHVB7_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1914063.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(20060..20341)
                     /locus_tag="ACHVB7_002075"
     CDS             complement(20060..20341)
                     /locus_tag="ACHVB7_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914064.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(20469..20703)
                     /locus_tag="ACHVB7_002076"
                     /pseudo
     CDS             complement(20469..20703)
                     /locus_tag="ACHVB7_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(21639..>22131)
                     /locus_tag="ACHVB7_002077"
     CDS             complement(21639..>22131)
                     /locus_tag="ACHVB7_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914065.1"
                     /translation="GLFILSGENKIDEECFINIVQTAVAINNIGRESNGYILVGVSDT
                     KATADRVKTLYGVTPIECNGYYINGIDHEAVIQSKNIDNYFLFIKQKIESFNFNEALK
                     QQILKDISLCSYEGLHVLKIEIKSVGEVCHFENRYYIRQGTATHLITDGEAISSLHRL
                     YYS"
BASE COUNT         5434 a         6004 c         5327 g         5366 t
ORIGIN      
        1 ttaaatccca ttggggtcac ggcagtgcgt tgctcattct gaagacggta gttattcacc
       61 ccctgaatga ggttaacaga gaggctgaga acaataagca ctgacataaa tataaaggcg
      121 atggccatta cacgactggt acttaaacgg gcaccgtgtt ccatataaca atcctggtat
      181 cagttctatt taatccactg ccggaaacac gaatcgggaa cattatgaaa aataccgcgc
      241 agcagggctg ttggcatata ccagtaaatc aggtcacgta accaggaact gccccgccct
      301 tttttcagtt ttttaatccc gaaataaacc agaaccgctg caccaatacc gaacagatat
      361 ttcgatgttg tgatacccca gccaacacag attgctgcgg ggatcagttc atccagtggc
      421 aggccaaacc agcggctctg gttggtcagt gtttccggga aacgatattt cttaagttta
      481 ttctcgtctc ccgacatcgt tttatttcct gtcagaggcc aacgacgctc atacccacag
      541 caataaatac agagatgatg gcaaagccga ccaggaactt gacgtttttg gtcatcatgt
      601 acatgaccgc accgaccagc acctcagcca gaacaaccca tttaacgata ctggagtcct
      661 taccgaaggt cgccttaacc gtatcgttac ctttagccat caggtcgcct ttaccagcag
      721 ccatagctaa ctcagggaac aacatcatca gaacagcaac cgggatcact gcacgaacca
      781 ggcgaagcat attcagacga gtgaatttag aaaaaaacga tttattttca acgggcgcag
      841 aagcaccctg aacacttaaa acagtattca tatgattcct ctgattaata ttgacagatg
      901 aaaactaaag ttctttaaat gttgactcgt tctcttcgat cacctccctg aatatttctt
      961 cattataaaa atctggataa cgttgcaaat gatcctgcaa cctcaccaat gcttcgtttg
     1021 ttttcgttct tccactacgc tctctggctc caagtaataa tttattcgtt tcctcatcaa
     1081 gggtaagtaa aacttttcta cttggcgcag atcttaaagc tcctttaatc accctccctc
     1141 ccataaataa aagagattta gtcaaaatat tcacataaga cgcacacaaa aataaacaac
     1201 aaaatagtaa attacgttaa caaaaaccgg cacttttagt gccgggaatg cttacatttc
     1261 tttacagtga aggctgttaa tattactagc gaagtattga tatagataaa ataacacatc
     1321 agagattata gcatatcgca accactcatc cctgtctgag aaaccatagt gttttttaaa
     1381 attcataaat atttttttag attggtcaac agagatatcc attctctgag aagaatactc
     1441 atgactataa cccaaaagat acggtattat cttattaaag cgatatttgt caggttcaca
     1501 accaaactcc ttgcccttta taaaaaagcc actaactacg tatctttcaa agaatctcca
     1561 gacaaacaaa ttcaagttat tcacttccgt actttcaacc accacatccc atgatacatc
     1621 attcaatatt acaccattaa taatactcac tccattcgag gacgagaaag catcaacttc
     1681 agcagtatga aataaaattt gcgtatcaat atcaatacca gaaaaccata tttcagaatc
     1741 actattctga cactttatta agtgtaaaaa aacagagttt gagtctatga attttcctga
     1801 ctctgtgcgg acacagactg gatattttgc ggataaaata atttccggca ggcctgattg
     1861 acgtatgagt gctctttctc tagtatccat cgcacacata ggaacctcct caaaggattc
     1921 tatggacagt cgatgcaggg agggacaagc tccctgcatc ggcgattttc aagttcaaat
     1981 tttaacgtgg cattaaccac gtaattaata tactaattat tctgattatt gtcatcagtt
     2041 tgaaccatta aaaagtctga tatttgaaca aagtcaaaat ttatttccct ttattcatca
     2101 tcattttttg gaaagaacct ttccatctca gccgacacct tctctctgat atcttcaacc
     2161 atattggcat attcaaactt tggatttcct gagacatgag gactgagaga ctcaataccc
     2221 aaaattttcg ctactgatga ctgtgttttt acaacgcatt caagaagtag tttattaaac
     2281 tcggcctgat taaatgcaga ctctttgcgc tccatctgag cctcatatac acgaagaccc
     2341 agttcaagaa gcattgaagc cgttgctgaa aaactgacat ctttttctct cgccccctcc
     2401 tgtcgacgtt tttcaataat cgcgtttatt ttttcataag catcattgct gatatacaga
     2461 ttcaccttag ccattagata ttaccttttt taaacataaa acaaagttaa tttcgaaaca
     2521 ctagaattca tagtgcaaac agggacgcac cgctagcagc gcccctgacg gtatcctata
     2581 aaaaaacaca ccgcgccgct agcagcaccc ctaatataaa ataatgtttt ttataaaaat
     2641 agtcagtacc acccctacaa agcggtgtcg gcgcgttgct gtagctgcgt taacgacgct
     2701 gctttaaaaa aatcagattt aaacaatata aatccacaaa tacaactcaa tgatattaaa
     2761 gataaatcag caaaaacttg tttttgcgta gtgtgtggtg cttttggtgg tgagaaccac
     2821 caacctgttg agcctttttg tggagtgggt taaattattt acggataaag tcaccagagg
     2881 tggaaaaatg aaaaaatgga tgttagcaat ctgcctgatg tttataaatg ggatctgcga
     2941 agccgccgat tgctttgatc ttgcaggtcg ggattacaaa atagacccgg atttactgag
     3001 agcgatatca tggaaagaat cccgttaccg ggttaatgcc atcggtatta atccggtaac
     3061 gggatatggc agcggactga tgcaggtaga ttcccagcat tttaacgaac tggcccgcta
     3121 tggaattaag ccggaacatc tgacaacaga tccctgtatg aacatttata ccggtgctta
     3181 ttatctggca atagccttta aaaaatgggg cgtctcctgg gaggccgttg gtgcatacaa
     3241 tgccggattc aggaagaccg aacgccagaa ccagagacgt cttgcctacg catcagaggt
     3301 ttaccggatt tatacctgga taaagagcag taaaggcatc cgggttccga ccacgaagaa
     3361 atcactttcc caaattaaca gtgtgcagaa aaattaatat tgtatacatt gtcaaatacc
     3421 ggttatgccg cgacagcggc atggaaggcc acacacaaaa atatcatatg agtgataatg
     3481 ctgactggcc gaagccccgc aaagcgggat cccatcttat atatatccgg taactgcgca
     3541 gcctcctgcc agaccatttt tcgcgggcac aatatttaca gactaccgaa aagccagttg
     3601 atgaaattga ggcatatcag acgacacagc aatgccgcag gatgataata taagtaaagc
     3661 cccgaaaaat ttttcggggc tttacttata ttatcatgac ttccactccc tgaactgttc
     3721 ggcaatcatc cataatgctt tgttgattcg gatatcaccg tcaatgcctg taatggctct
     3781 tgttctggta tttttaccgg aggcacttcg ccccgataat ccacctttta tcatattctc
     3841 ctgaacccgc tgccaggttg tccagagatc gttctgttta tcctcccaac ggcggggagt
     3901 aattatttgt tctggcgtca ctggcgtttt attttcatct tcgtacctgg ccatcaatgc
     3961 agctctgcca aataaatgtt gttcgtcact gttaagatgt atctctttca ttgtctccat
     4021 attttcagtg actttatcaa agacaccgag cacttcatac gctccctcaa taacctgccc
     4081 gacaatatca cctttatgtg gaacgcggat ttcgccaaaa ttattcccac acaccaggcc
     4141 atttgtgcag acaaaacgaa aaattcctgg gatcatctga taactggatg aaccatcatg
     4201 tgaattaagc aggataattt caggaacttc tttcccgtta atatgccctt ccctgcgaag
     4261 acgtaacata tgtttactgt attcgcggcg tcccaaatca cgaacccgac tctgacaggc
     4321 aaagaatggc tggaaacctt catcacgtaa cttattgatg atattgatgg ttgggatata
     4381 tgtataacgc tcactccggg attcatgttt atcaccagaa aagacagatg gaacaaactg
     4441 cattaattcg tcatccgtta acgggcgctc acggcgaata gaattatatc gaccaaaacg
     4501 ggaagctaat cgcataacag taactccttt atgaaggcat aaaattaata aaagtttttt
     4561 cgcttaatat catcccccct cggttatcgt taacaaaaag acaacaattc cgaggggaag
     4621 gaccatcaaa tcaggtcagt tcgggactga ccaacctgcc gagtgaaaag cagtaacagc
     4681 gcgatcaact catcagcgtt ttttctgaca agaggagtat gattctccag gctatccagt
     4741 aagtcatgca gctcattatt gacctggtgg agcaccttaa agagtgcacc agaatcccgg
     4801 cagctctgat atgcaagatt tgcctgaaca gcacgattct gcatgatccc gatttgtgcg
     4861 attgcgtctt tctgctgatc gcctgttggc tgataagtgg tgttaaaaga aaatgcgctc
     4921 ataattcctc ctgtatctga tgttttcagc ccccatcatc tgttgagttt cgggagccgc
     4981 ggttttgacg ttttgcgggg acctgccttt tgttccctcc ggtattcacc atcacctgtt
     5041 tgagaacctt cgccgcaagg ggcatcactg ccacggcctg acgcacctgt cctgggcaga
     5101 acgcaaaatt ttttaccggg ttttgggtaa aaaattttgt gggctgttca tttaccctgg
     5161 acagtcagtg cggcaggttg tggctgtcta tgcccccggc gacaggggct taaacaggtg
     5221 aatggcaccg gacagaacaa cgggcaggga cccgccgcaa cgacgtgaag cgcggttcgc
     5281 gaaacggcgt tgctccttac atcccggcgt cttttgccgg gatgccggga gcgcaggcca
     5341 cggcgcgaca agggtggcgc gaccgttccg gtcgctgcaa acccgggttt ttgcgtcagg
     5401 gatggaagcc cgccagggcg gagacagctt cgctggctcc gttcacgaca gcccgccccg
     5461 ccagggggac gcccggaaaa ggcataagca ggagaacaac aaaacagaca ataatcaggg
     5521 cagcagtaat cagagtcaga gcagtaatca gagtcagagc aggaggaaaa agagaataat
     5581 caaagggaag gtgatgtgat ttctggtaag tcataaacag agaaggcggg ggaaaagaac
     5641 gggcgccgca ggcgcacgtt catccccgtt cccggaggga aacggccttc ctggcagtct
     5701 ggttgttcat gcccgtacaa tgccagcaag gagaaagggg ccaccggcga accagcagcc
     5761 cctttataaa ggcgcttcag tgcagtcaga ccagcatcag ccctgaaaag gcgggcctgc
     5821 gcccgcctcc aggttgctac ttaccggatt cgtaagccat gaaagccgcc acctccctgt
     5881 gtccgtctct gtaacgaatc tcgcacagcg attttcgtgt cagataagtg aatatcaaca
     5941 gtgtgagaca cacgatcaac acacaccaga caagggaact tcgtggtagt ttcatggcct
     6001 tcttctcctt gcgcaaagcg cggtaagagg ctatcctgat gtggactaga catagggatg
     6061 cctcgtggtg gttaatgaaa attaacttac tacggggcta tcttctttct gccacacaac
     6121 acggtaacaa accaccttca cgtcatgagg cagaaagcct caagcgccgg gcacatcata
     6181 gcccatatcc ctgcacgctg accacactca cttttcctga aaataacccg ctcattcagg
     6241 ctgtttacgg gtaatctgtg tgattgttgc cgcatcacgc cgcctcccgg agtttgttcg
     6301 gcaccatcca ccgctccatc tcacggacag cgtgaccggc ctctcttgct gcaagactgg
     6361 cttccgcaat aacctgccag aacggcatat cctgccactg ccagcgttcc attccggcca
     6421 gtgacggaaa acgctcaccc cactgccaga acagggtttc caggtcatgc tctgccaccc
     6481 ggtcactcca gcgtgcatac cagcttccga ctgtttccgg cgaatggaat gccagttcga
     6541 tttcagcctg agcgaccagc gcctggtacg cacgggcttt ctgctctgcc tccctcgcct
     6601 gctggcggct gtacttctgc atgaccagcg cggatttatg gcgactgcgt tccctgacac
     6661 gaaaccgcac cgacgggaac aagcgttctg ctgcatcgcc tggaagagga aggaggcaga
     6721 cctcgccctg tgtttcaatc agcacatcga tggcatacag ccactgaaga cggctgccgt
     6781 gcagttcttc tgcggtcagg gacggcatga aaaatcgcag gtcagacagc gatatcctgc
     6841 ggcttccgtt cagacagcgg aaaaacgccc ccgcatacgg atattccgcc agcgtgtttc
     6901 cctggtggcg gcgtttttct gtctccgcca ccgcccgcca tgcggcctgt tgctctgcgc
     6961 tgaggggaac cagtgcctgt gaacgtgcgc tcatgcctga cctccttccg ccatcaggat
     7021 atggatgatg tcactgacgc tgtacccctg cgcgtccagt gacgccgcca gtgccagtga
     7081 atggctgacg gcttccggtg caaaccgccg ctgtacctga accaccgcaa acggctcacc
     7141 accggcattc accagaacca gcacccagac ctgagagaca tcgcccggtg aacataacgc
     7201 caggtgaaaa cgttcgtggg caggcgtgaa acggacctgg acggggaaaa atccgccaaa
     7261 ctcgctgccg tactcgccgt tcatcgtcca gttgtccggc gcatccagtt cccgcatcac
     7321 ggcgtgtgtc agctcacgac gttcatcact tccggcagcc cggatatctt catattcctg
     7381 tgcgctcatg gtctgtaata cattcagggt cagttcagtt ttcatcacgg tatctccctt
     7441 gctgccgccc cgtctccggc ggggcggtgt ggttgttcat caggcggcat cagccaggtt
     7501 atcggtgcgt tcagtggtat ctgtctgtgg atgtggtgca cacatccagc caggcaccca
     7561 gcggttgtct ttcatatggt gctctgcatg ttcagccgca tcacctttct tcatcttctc
     7621 cgcgtcccgt gcggcaccgg acagccctgc ctcattcagg gctgcgataa tctgcggctt
     7681 tttcaggtgt ccgaagaagt ttgcttttgt cggctgccac cagtcgcgca tgtgaaagcc
     7741 gatggcgctt tccagcgtgt caagcggact gcgtgacgtg tgaccacact cacgggtctg
     7801 gacaccgtta aggctgcatg cggtgcagaa actgagcagg gataacagca cctcctgact
     7861 gagtgacagg aatgtcgtca tgtcccgtga ccatccctcc ggtaacaggg cagcaagacg
     7921 ggctttttct gccatcagcg ccatgaatgc ggcaccttcc ttccccgatg gcgcatcgct
     7981 ggtcagcgaa taatgtttac attccaggct gatttgtgct ggtttactgt acgctccgct
     8041 gccaaacaca ttcaggcaga gcgtccatgc cagcagtgtc agggatttgt ccggttgctg
     8101 catcagtgct gcctggactg ccagcgtgcg ttcggaagac attttcgtca gcagtggcag
     8161 actgatttcc tccactgacg ctttctcctg cacctgttcc gtacggtcag cgtcgtcagc
     8221 cacgtcatcc tcactgcgca actgcacacc acgctggaca cacacgttgc cataacgcca
     8281 ggacaccacc actccacctc cggcacgcat ctccggcgtc cacgccctga ccttcgccat
     8341 gcagcgcatc agcttcattt ctgcttccag caggtcagat tcctcacact ggttttccag
     8401 cgcgtcgtaa cgcgtcatca gttcgttcag acgttcttct tccgcctccg tcagcaccgc
     8461 ttccggctcc ggcagacagc ggtatgttcc ggcatcctca cggcactcac cgacaggctc
     8521 catgcgtccg gcgcaccatt cccagccttc ggcttcccga aggaactctg cgacagcctg
     8581 gagtttttcc agcagggcgg catcgagcgc cacgcagtcc acatagccac cttcgtcgtc
     8641 gctgaacaaa tcggtgcgca gttcgtctgg cgagaaggca tcagccccca cgaagcggaa
     8701 tttactgttc cccgccaccg ccacttcact ttcggtcacc agacgacgaa tggtctgtac
     8761 ttccggttta ccgccccatc ccgactgaca ggcggcttca aacacctgca cctgacgcgc
     8821 ggtgtcgttc tccagcgcca gcgcctgaca gtgttcggtg gtgatgcggt cttctgccag
     8881 cgcatcgagg atgacaggcg caaggtcagc cagtttcagc attcgctgaa cgtggcgggg
     8941 tgaatagccc agcaaatcac cgatttgtgc aggtgttttg ccttcctgcg ccattgcgcg
     9001 gaatccggca atctgttcgg cagggtgcat atcccgacgc tgaccgtttt cagtcattga
     9061 tgcagccgtc gccagctcct gcggaataac cttcacgcgt acaggccagt cagccggaag
     9121 gatgccacgc tctgccagca tgttgagtgc tgccagtcgg cgaccacctg cggcgacacc
     9181 gtggcggtca ccaggcaggg catgaacaac cagattctgc aacaggccaa cgcccttaat
     9241 ggactccgcc agttcgctga cggactctgc ggaatacggc accgtgcgca cattcagcgg
     9301 tgatttaatc agcgaggcca gcgacacgct cacttcctcc gtctgctcca gcagggcaga
     9361 caggactgtt tcctgcgttt ttgcaggttt acggctggat ttacgctccg tttttgtctt
     9421 agactctgta actgacatat atttctcctt tgaccgggat ggttgtatgt ctgccggacg
     9481 cgttcccgtc cggcacctct cttacttaag aatgccgaaa ttccggatga catggttcat
     9541 ccagtacccg gcaccgtcct caaaattcca ggtacgccag accaggccac tgttattgcg
     9601 gacaaccagg cgaaaatgcg ttccctgatc atcctcgata aagacattcc ggtactgtgc
     9661 ggcaacggct tcagcctgtt cgcgggtaaa ggagccgtca ggcagcccct gaaatattct
     9721 gaaatattcg ctcatcctca tatctccggt gatgccccgc actggacggg gcggttgtca
     9781 ctgtctttcg ttagaacgga atgtcgtcat cgaagtcata cgcgggttca gacggctgct
     9841 gaggctccgg ctgcgcggcc ttacgtccac ggccttttgt tttcgcgctc ccttttttcg
     9901 tcgtgtcagc actctgtggc tgaccattct gctgaggctg cggttgcgcc tgagcgttct
     9961 gctgtggtgc actccccagc atctgcatgg tgcccgtggt tttcacaagg atttcagtga
    10021 cgtaacgggt aataccttta tcttcccagc tacgggttcg gagttgcccc tcgatgtaga
    10081 cctgcgcgcc cttgcgcaga tattcacctg ccacttccgc gagcttgccg aacagcacca
    10141 cgcgatgcca ttctgtctgc tcccgcatct cccccgtctg tttgtcacgc cagctttctg
    10201 acgtggccac ctgcaggttt gccactgcgc ccccgttggg gatgtaacgg acttccggat
    10261 cttttcccag acgaccgaca agaatgacct tgttaatgcc acgaactgcc atgatgtgtc
    10321 tccttctgtt gatgttttca gcccccatca tctgttgagt ttcgggagcc gcggttttga
    10381 cgttttgcgg ggacctgctc tttgttccct ccggtattca ccatcacctg tttgagaacc
    10441 ttcgccgcat ggggcatcac tgccacggcc tgacgcacct gtcctgggca gaacgcaaaa
    10501 ttttttaccg ggttttgggt aaaaaatttt gtgggctgtt catttaccct ggacaggtgc
    10561 ggcaggttgt ggctgtctat gcccccggcg acaggggctt aaacaggtga atggcaccgg
    10621 acagaacaac gggcagggac ccgccgcaac gacgtgaagc gcggtttgcg aaacggcgtt
    10681 gctccttaca tcccggcgtc ttttgccggg atgccgggag cgcaggccac ggcgcgacaa
    10741 gggtggcgcg accgttccgg tcgctgcaaa cccgggtttt tgcgtcaggg atggaagccc
    10801 gccagggcgg agacagctcc gctggctccg ttcacgacag cccgccccgc cagggggacg
    10861 cccggaaaag gcataagcag gagaacaaca aaacagacag taatcagagc agcagaaaaa
    10921 agaagaacag tagcagtagc agtagcagta gcaggaacaa caacatcatc agtaacaggc
    10981 aaatgacagg tctgaaaaga cagaagccca cagcagaacg tccgcgtaat gaaaggggcg
    11041 gcgcgtaagc gcggcccctt tcagcgccac cggcgcgcgg tcttttcccg gcgcgggcag
    11101 acgggcattt atcctcccgc ctgcccggat tcatcaggca cgcagacgaa taatcatcgt
    11161 cggaacacgg gtataagcaa atgtgccgca cggcagcacc tcgcggacat cagaaaacgg
    11221 taacagcttc tcctgctggc gcggcccgtt cagacagacg gcaaccagaa cgccccccgg
    11281 acgcaacagg gaaaaagccc gcaggatatg ccggatatcc tgcccgtgac tgaacggcgg
    11341 attcataatg atccggctgt aatactgcac cggctgccat tccatgaagt cgccacactg
    11401 taccctgaca ccgttaaagt tttcccgcag ataccggacc agcccgctgt taatctccac
    11461 cgcatcacac atggcctccg gcgcggtatc ccgaatcgcc cgcagtatgg ccccggtgcc
    11521 ggcagagggt tccagaatgt gatcacggtt gctgatttcc gccagcgtca ccaggcgatc
    11581 acagaccggg gcaggagtca caaaaagctg agattcagcc cggatttcag caaccggcgc
    11641 ggcgttatcc agccttgcgc gcatcagccg gatacgttct cttaactgac tgtgcatgtc
    11701 attatctccg tcatcccccg gggatttccc ggggggatat accttatttc tgcgggattt
    11761 ccgtgatttt catgtcggtg atatacacat tcaccagacg gaaattattg tccattgtgc
    11821 ggcggtagcg gtatgccccg tggtcttctg cttcttccac actgcgcacg gccttacagt
    11881 cccggggcag ggcggcccac tgcgcctttg tcatttcccg gaaaccttcc cccggatagt
    11941 tgactaccgg cggacgcttc gcggcctgac tggcgacggc agcctcttct gccgatggtg
    12001 ctttgtagtc cgtgatgcga tcgggcgtca ctttcatcgt gccgctgtac ccgagaaaac
    12061 tgtaatttgg cgttgtgact gaactcaccg ccccgttgct tttgttcacg cggatgatag
    12121 tcagccactc gccccggctg aaaacctgtc cgcccggctc aaaatcctgt gtccgggtaa
    12181 ccacgccgcc gctttcgtcc agcatggcac gctcatagat caggcggttc tgatagtggt
    12241 taacccagcg ttgctgatgc tgaatctgcc gttcatgaca gcggatcgca atttcgcggg
    12301 cctgctccgt ggtgatgatg tcatcatcca gggccgacca taacgaccgg ctcccttcat
    12361 actggctttt ttctgccggg cgcggatacg tatccagcgg gaaacaggcg ctgatatggt
    12421 catgactgct gatgagtttt gccatattca gatccagcga ttcagcccgc cacatcgtca
    12481 gatatttctg cgactgtgcg atggtctttt cagccttgcg caaatccgct tcgatttttt
    12541 tgatacgacg ccagcgaacg tccgggcgtt ctttatactt cgcgtgaagc agtgccgacc
    12601 gcgcccgctc ctcccagtat tccgcacgtt caaagagcat cacggcacgt ttcatgccgt
    12661 tttcaatacg ctgcgcatca cgacgggcgc ggcgttcgct gtgatgcccc acaagaatgg
    12721 gctgaccggg cgggatcatc gcggccagtc tttccacttc atcaagtgcc tgtgcggatt
    12781 cactggcccg ctttccgctg tatccggtaa accgctccgc ccgtgcttcc tgacgttcag
    12841 cgagcgtgct gtcttcatcc tcaatctctc cggcaagtga gaggagcacg tcttcccggc
    12901 ccggtgtcca ggccggcgcg acaaacagcg cctgtttggg tgcccagcga aaaccggcat
    12961 catgcacttt tttatacgtc tccgggtcaa gacgtgacgc ggcatacagg cgcagtttgt
    13021 tgtcatccgg tgagtaggtc gcgcggtaca tctgcacagg gctggcggct tccggggcgg
    13081 tcatggtgat tgctgctaat gtcataaaat gttctccttc cgtctgttgc cgcccctcgc
    13141 ggggcggctc acctcatcag ttaaaagcgc gtttcagttc gagtgtgcgg agcacctgcc
    13201 cgaaagccgt gtttttcatg gcggggatgt gtacaccacg cccccggtga aaactccggg
    13261 ctatgtgtcg cccgaaggct ttcaacacgg ggtcaatttc cggcgtgggg atgtcttcgc
    13321 gtctgaaacg gggatgacgg cggggcttta cagccgctgc ggaaggtgaa acaatgctta
    13381 cagtcataaa tggattcctt atgacaaatt ggttaacaat gccgtcaaaa ttggtcgcac
    13441 aattcttacg gcgtggatta agcaggtgcc tgacctgctt ttttcatatc tgcatctgac
    13501 gctttccggt gttttcctcc ttctgttcct gcgttctcat tacctgtcag ccggatgact
    13561 gacagaacaa ataaatgcgt attgccggag cctgaaataa atcctgattt ttaaagaaca
    13621 tcacccgttg agtttcggga gccgcggttt tgacgtttcg cgggaacctg ctgctgtatt
    13681 cactgtggta tgaacttcac ccgtttgaga accttcgccg caaggggcat cactgccacg
    13741 gcctgacgca cctgtcctgg gcagaacgca aaatttttta ccgggttttg ggtaaaaaat
    13801 tttgtgggct gttcatttac cctggacagt cagtgcggca ggttgtggct gtatcagccc
    13861 ccggcgacag ggactcatac gggtgtgtgg cacccggcag gcaatgagca ggggcccgcc
    13921 gcaacgacgg agagcgcgtg cgcgaaacgg cgttgcccct tacatcccgg cggccttttg
    13981 ccgggatgcc gggagcgcag gccacggcgc gacagaggcg gcgcgaccgg aacggtcgct
    14041 gcaagccagg ggttttcacg catcggaccg tagcggcagc ccgaaaccgc gccagcgggt
    14101 tcggcggaga ctggcaggtg cgccagcagc tgcctgtcgg agtcgggcct gacgaccgga
    14161 tgccgccagg cagccgggag ccgaaggtgc gcccggcaga cagaacaaca ctgcagtagt
    14221 cagaatcaga gcagtagata aaggaacaac aacatcagga acagttccat aacataagaa
    14281 agaaacactc ctgagtcaga cacatcacag acgggaaggg taacgggcga cgcgccagcg
    14341 tggcccgctt cccttccggc aggcgtcagc ctgccagggg ttttcgcgcg ggctgacagg
    14401 cggcctccac cgcctgcccc tgtatcacct gtttatgctg ccctgaagga tgtagtgacc
    14461 gccgaggaat acagcctgct gattcgccac aaaatcgcag atttcatcaa caatctgcgc
    14521 agtggtgagc ggtttctgcc ccatttcacg acgccgcgca ttaatcacgg ctttgtaatt
    14581 gcgcagaatt tcgcgggttt ctgtgctgat attcatgaca tgtgctcccc gtccagcgcc
    14641 tgtaacacaa cgtccattgc ttcgcgttct tccctgacga atgcgtcacc gtcgcccccg
    14701 ttctcgctcc cgacggcata caggcgcttc atttcactgc ctgccagcgc catcactttt
    14761 tccagcatca ccgccggaac tgtcacctga cggctggcgc gcttttcttc catcagctca
    14821 cgaacaacgt cgtgaacaat gctgacatcc gcaccatgcg caaaggcatc gtccagtcgc
    14881 tgcatgacgg tttccagctc atcgtctgtc agactccagt cctgggctat gtgacgcaca
    14941 tccccacggg tcagcaccac ggcaaaaacg cattcactgt ccggcacgtc tgtgttcagc
    15001 acttcgcgga taatttcttt aacagtacag tacatgttct cgtctccttt acaggggcgg
    15061 cattgccgcc ccgtccgtta atcagtccag tgccgcgtaa atcgcgttgc attcggggtg
    15121 aaattcgatg tgacgccaca actgcgcatc gcgcatccgg taaagctggg tcagtcctgc
    15181 atccccgctg tcgtggtaca gccacaactg gcggttaatc acaagggaag tcagaatgat
    15241 gcctgcggca tcagcactca cggtacggtc aaaccagttt gcaccgttca ccatgtggaa
    15301 acggtcgcca tccggcatca tgtaaccgcc accgtccggc aggcggatga agtgccagaa
    15361 gcctccctga tattccggca tcattttttg cgccagcgcg tacacatacg cctcggcata
    15421 cagaaaatcg ttgccaaaca gatacggtag aaaggagaga cggtcgcact cttcaacgtt
    15481 cagagtgaca ggtttcgcat attgcatgat gttttcctcc gttgttcaca tattccgcga
    15541 caccgggcgg tgccgtgcct ccataaaagc cgggcgataa ggcggggatg cacagggcgc
    15601 aagggaagcc gcagcctgac gcacacggct ggcgggagtt ggagggtgag accgcgaaat
    15661 ctgagcgggt gaacgaccgg aaaatcacgg cagacgggaa ggagggaccg ggtgaccgca
    15721 gcggcgaagc gtacccttgc atccctgcca gcccggctaa gatggaggca cggcacagcc
    15781 cgcacacgac gctgaggcca cataactgaa aaaccacaag gccatgccct gcatggccgg
    15841 gtaaagcgga gccggcggcg acttcctttg tgctctgtcc ggggaccggc aggaaaaagg
    15901 cggggattta ccccgccaac gtcatcattt ccggattttt gccagggcat ccgccatgct
    15961 gcggatttcg gcccgggctt cccggaccac ctcccgcaga acaccacggt caaaccagcg
    16021 gcggggcggt tcatccggta tacgcagcac atcgcaggcc agcccactgg acaggatatg
    16081 ctcaatacaa tcgctgacca ggtacatttc caccgacgaa aaacgtgctc cccggttcac
    16141 gatggcatcg agcaccacgg gcaggttttc gtcatcttca tcgagccagc aggggtgatc
    16201 gctttccttc acaaccagac gaaagcggcg cgtctggcga gtcagcaacg tctgaccggt
    16261 ggtggtcagc gccgcccgga tacgccgcgc atcttctgcc acccggcaac tgacactgcg
    16321 taccgcatcg cgcaccgccg caagtacagc cctgatttcg tcttcggtca gccattctcc
    16381 ggcatacggg aaagacacct gcgttccgtc tgccggaagt accggcagcg actgccactg
    16441 actgccggac agtaccagtt ccgccccggt tccggtcagt ttctgaatgg cggaggccag
    16501 gagcggcggc agtacgccgc cgtgacggtt gataagtaac gtcgccagcc ggggcgcatc
    16561 cagcagaaca taaatgctga tccagcccgg atacttcggg ttacgctggt caacatccgt
    16621 ctcttccggc cagccctgcg ccagcagcag gttgcgggcg tgacgacaaa tcagtgcgtt
    16681 cagcgtgctg ttttcgttca tgattaagcg ttctccctgt atcgccgccc gcagggcagg
    16741 cggcgataac atcagtcgtt ttcgtgtgtg ttgtttttca gcagggtgcg ggcctgatta
    16801 accacctcct gacggcgggt gacgcattcc gggacggcga ggccatgatc ccgggcggcc
    16861 cattcacgtt tccacaatgc ctgttcagcg gtctgaatca gggacgccag cagttcagca
    16921 gggacggtca ccaggcggtt ttcgctctcg ttgcggataa tctccatcac cgcagcggaa
    16981 gaaataccgg actcaatccg ctggtcttcc gggatgtcct ccaggcgtgc caggacggtc
    17041 ctgatttcat gaccggtcag agaaatttcc ataccgtcag ccagtgcctg aatctcttcc
    17101 ggggaccaga taaccagcat cagcggcatg tctcccggat actttactgc cagctcccgg
    17161 catagcgttt cgcatgttcc gtacattctc gtctccttct caatctttaa agcacgcttc
    17221 cagttccaga atttcggtat atgcctccat caggaggcgg tttgcgcgaa acagcgtctg
    17281 acgcaccaga cgttccggca gggtgtcggc attaaagctg taccgctgct ccatctcatt
    17341 catggttgta cgcaggtcgc acatggcatt ggccacctcc gccacttcgg cgcgcagttt
    17401 ttcgtgtcct gcatagttca tttacgcagc ctccggcata aaccagtcat cattcgcggc
    17461 cccctgctgc atggcccgtt gcacggcggc aaggcgttgc tgcccggcac ggtgatactg
    17521 ctcaagcagc tcgataccga tataccggcg tccggactgg agggcggcga cgcaggttga
    17581 gccgctgcct gcaaacgggt ccagcacaat tgcgttcggg tgtgtgaagc tctcaatcag
    17641 cggttgcagg ctggtaacgg gcttttccgt cgggtgatgg cgattgcccg aatatttcca
    17701 gcccagcacg tccggcagcg ggttttgtgg cagacgtgga cggccttttg ccaggatgta
    17761 ggcacattcg tggcgatagc ccacatatgc ggccttcgat gtgtagtttt tggtgaagac
    17821 caggtgacca acaacgctga atcccgcatt tttccaggcg gccataaagc gatcgacgcg
    17881 gttccagcca tagaagctca ccattaacgc gtcttttttg agtacgcggt acatttcatt
    17941 acaggctggt tgcagccatt catcggtttt atcgccggcg atggtgcgcc cggaacggtc
    18001 acggaaaccg acgagatacg gcgggtcggt gaggatgaaa tcgacagcat tgcccggaaa
    18061 cgtggccata acacgcacgc agtcacccag gataaaacgg gacataacag cactccttct
    18121 cttacagagg gcaccgggag ggcacccctg tggtgcccgt tccccgctgc ctgtgggctg
    18181 ttcaccgcgt ccggtaacgg gtcaggccga ccgtggaata ccgcagccgt cgtctttttg
    18241 acggcgagga tatgccgcga aagcggcttg aagggcgttc atcagaaaag ggcgacgggc
    18301 tggcgtacac tgtcaccaac gccagccggg agcggtaaaa gcgcccgtcc cgtgcccggc
    18361 cggacgcggg acactgacca caggcagcgt tcctgccgcg ccgcgtcagc cggcgcagta
    18421 aaaagaggag cccggcgaca tcttttgtta cccgccaaac aaaacccaaa aacaacccat
    18481 acccaaccca ataaaacacc aaaacaagac aaataatcat tgattgatgg ttgaaatggg
    18541 gtaaacttga caaacaaacc cacttaaaac ccaaaacata cccaaacaca caccaaaaaa
    18601 acaccataag gagttttata aatgttggta ttcattgatg acggttcaac aaacatcaaa
    18661 ctacagtggc aggaaagcga cggaacaatt aaacagcaca ttagcccgaa cagcttcaaa
    18721 cgcgagtggg cagtctcttt tggtgataaa aaggtcttta actacacact gaacggcgaa
    18781 cagtattcat ttgatccaat cagcccggat gctgtagtca caaccaatat cgcatggcaa
    18841 tacagcgacg ttaatgtcgt tgcagtgcat cacgccttac tgaccagtgg tctgccggta
    18901 agcgaagtgg atattgtttg cacacttcct ctgacagagt attacgacag aaataaccaa
    18961 cccaatacgg aaaatattga gcgtaagaaa gcaaacttcc ggaaaaaaat tacattaaat
    19021 ggcggggata cattcacaat aaaagatgta aaagtcatgc ctgaatctat accggcaggt
    19081 tatgaagttc tacaagaact ggatgagtta gattctttat taattataga tctcgggggc
    19141 accacattag atatttctca ggtaatgggg aaattatcgg ggatcagtaa aatatacgga
    19201 gactcatctc ttggtgtctc tctggttaca tctgcagtaa aagatgccct ttctcttgcg
    19261 agaacaaaag gaagtagcta tcttgctgac gatataatca ttcacagaaa agataataac
    19321 tatctgaagc aacgaattaa tgatgagaac aaaatatcaa tagtcaccga agcaatgaat
    19381 gaagcacttc gtaaacttga gcaacgtgta ttaaatacgc tcaatgaatt ttctggttat
    19441 actcatgtta tggttatagg cggtggcgca gaattaatat gcgatgcagt aaaaaaacac
    19501 acacagattc gtgatgaacg ttttttcaaa accaataact ctcaatatga tttagttaac
    19561 ggtatgtatc tcataggtaa ttaatgatgg acaagcgcag aaccattgcc ttcaaactaa
    19621 atccagatgt aaatcaaaca gataaaattg tttgtgatac actggacagt atcccgcaag
    19681 gggaacgaag ccgccttaac cgggccgcac tgacggcagg tctggcctta tacagacaag
    19741 atccccggac ccctttcctt ttatgtgagc tgctgacgaa agaaaccaca ttttcagata
    19801 tcgtgaatat attgagatcg ctatttccaa aagagatggc cgattttaat tcttcaatag
    19861 tcactcaatc ctcttcacaa caagagcaaa aaagtgatga agagaccaaa aaaaatgcga
    19921 tgaagctaat aaattaattc aattattatt gagttccctt tatccactat caggctggat
    19981 aaagggaact caatcaagtt attttcttac cagtcattac ataatcgtta ttatgaaata
    20041 atcgtttgca ctgtctctgt tattcaggca atttcaataa aggcacttgc tcacgctctg
    20101 tcattttctg aaactcttca tgctgcattt cgatttttct tctaaaatca taacttgcat
    20161 acctgttctt tttatgtata tccagatcta tttttgtgac gagttcaaac cacaatgata
    20221 gcactcccca tgcagcgtta tgaaacattt ccgcaacaga tttagagtct gtattaaccg
    20281 cttcagtcat gtagtcgcta atacgttctg cagtgatttt cgttatttca tcgtaattca
    20341 ttttatcccc tacaagacta tttaatggaa tgaaaaatat ttggtctgat aacaatgctc
    20401 ctgaattaac acctgcaggc tatgttaaag ttgccctcac acacaatcta cctgttttct
    20461 cgtgtgtgtc acaggcgcaa tacataaata gtctggccat taagttcgta acggatttcg
    20521 tactcgccag caaaaatccg tctgacatcc ctggtttcaa actgaaacaa ctgttcatcc
    20581 catacgtgga tgagttaaca gaatgaccgg cgcctgtgtc agggactaca ccgttttggc
    20641 tgcagctggt ttactgacca acgccagaaa atcatataac tgcgtcaatg tcagcgtatc
    20701 cagcgcgact gtattatttt tctctgcaac cagaatattg cattaggtta ttaccaggtg
    20761 ttctaatgta caggtaaaga cgttctttat aatctgaaat ctctactttt aatccctcag
    20821 actttgcctg ctgaaagccc tcgtcaaggt gcgcttttat taggttaatg tctggatgtc
    20881 ttgtatcggg ggcaattctg tattggtgta acgatggggt ggatgttccc tgcaataaga
    20941 cctccagata gagattggca caaccattcc cattatttct aatagtttat tggtaatcac
    21001 aaactgtata tttagccatt atattgaatc ctgtgaagta aacggcgacg gctcaaaatt
    21061 ttatcaaacc taagataatt ctaccatcaa agttaataat ttgaatttat atgacggctc
    21121 tatggggggg taaaaatccc cgtgcagaga ggcgattttc agagttgaaa acgtcgtcgt
    21181 taagaacacc aaagtcagta ccaccaaccc ggaaagtagc ttcatgcacc gggacaacag
    21241 cagttttggt gtccgaaatc gtacaattct gtacatctac gcaaggaaat gtacggaagt
    21301 aagccaaatg gttatcggac ggtcggaaca tttcgtacag cctttacgtt cagtgagtta
    21361 gctggcctag atgtaaccac tcgttcaact caattctctt agcgtgggaa aatttccgtt
    21421 ttccaagcgg ccccctcaag cggacccctt atagtacaat aattttcgat atccaaactg
    21481 cccccaatta catctccagt ctctggcagc agagatcata tatttccatt gccgccgtag
    21541 ccacgatatt ctggctattc acaaaaacaa tttagcccca aagcgcagta aaaaagtaca
    21601 ctatgaaggt gtatgatacc ttcatagtgc ttaagtaatt atgagtaata taatctgtgt
    21661 aaactactaa ttgcttcacc gtcagtgatt aaatgcgtag ctgttccttg tcttatataa
    21721 tacctatttt caaaatgaca gacttctccg acacttttta tttcgatttt taaaacatgc
    21781 agtccttcat atgaacataa tgaaatatct tttagtattt gttgtttcaa agcctcatta
    21841 aaattaaatg attctatttt ttgttttatg aacagaaagt aattatctat gttttttgat
    21901 tgaattacag cttcatgatc aattccattg atataatagc cattgcactc aataggggta
    21961 acaccatata aagtcttaac tctgtctgcc gtagctttgg tatcggaaac accaactaaa
    22021 atataaccat tagattctct accgatgtta tttatggcga cagccgtttg gactatatta
    22081 atgaaacatt cttcgtctat tttattctcg cctgagagaa taaataatcc c
//
LOCUS       DAZPJR010000058        21641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPJR010000058 DAZPJR010000000
VERSION     DAZPJR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.059"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1125)
                     /gene="fsr"
                     /locus_tag="ACHVB7_002078"
     CDS             complement(<1..1125)
                     /gene="fsr"
                     /locus_tag="ACHVB7_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR1914066.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPRSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1343..2995
                     /gene="ushA"
                     /locus_tag="ACHVB7_002079"
     CDS             1343..2995
                     /gene="ushA"
                     /locus_tag="ACHVB7_002079"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR1914067.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3032..3511)
                     /gene="ybaK"
                     /locus_tag="ACHVB7_002080"
     CDS             complement(3032..3511)
                     /gene="ybaK"
                     /locus_tag="ACHVB7_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR1914068.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3715..4509)
                     /locus_tag="ACHVB7_002081"
     CDS             complement(3715..4509)
                     /locus_tag="ACHVB7_002081"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR1914069.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4647..4988
                     /locus_tag="ACHVB7_002082"
     CDS             4647..4988
                     /locus_tag="ACHVB7_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1914070.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5203..7707)
                     /gene="copA"
                     /locus_tag="ACHVB7_002083"
     CDS             complement(5203..7707)
                     /gene="copA"
                     /locus_tag="ACHVB7_002083"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR1914071.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7969..8901
                     /gene="glsA"
                     /locus_tag="ACHVB7_002084"
     CDS             7969..8901
                     /gene="glsA"
                     /locus_tag="ACHVB7_002084"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR1914072.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8904..10196
                     /locus_tag="ACHVB7_002085"
     CDS             8904..10196
                     /locus_tag="ACHVB7_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1914073.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10227..10324
                     /locus_tag="ACHVB7_002086"
                     /pseudo
     CDS             10227..10324
                     /locus_tag="ACHVB7_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10321..10728
                     /gene="cueR"
                     /locus_tag="ACHVB7_002087"
     CDS             10321..10728
                     /gene="cueR"
                     /locus_tag="ACHVB7_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR1914074.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10729..11187)
                     /locus_tag="ACHVB7_002088"
     CDS             complement(10729..11187)
                     /locus_tag="ACHVB7_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1914075.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11184..12101)
                     /locus_tag="ACHVB7_002089"
     CDS             complement(11184..12101)
                     /locus_tag="ACHVB7_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1914076.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12247..12924
                     /gene="fetA"
                     /locus_tag="ACHVB7_002090"
     CDS             12247..12924
                     /gene="fetA"
                     /locus_tag="ACHVB7_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR1914077.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12911..13690
                     /gene="fetB"
                     /locus_tag="ACHVB7_002091"
     CDS             12911..13690
                     /gene="fetB"
                     /locus_tag="ACHVB7_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR1914078.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13753..14607)
                     /gene="cnoX"
                     /locus_tag="ACHVB7_002092"
     CDS             complement(13753..14607)
                     /gene="cnoX"
                     /locus_tag="ACHVB7_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR1914079.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14668..15477)
                     /gene="ybbO"
                     /locus_tag="ACHVB7_002093"
     CDS             complement(14668..15477)
                     /gene="ybbO"
                     /locus_tag="ACHVB7_002093"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1914080.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15467..16090)
                     /gene="tesA"
                     /locus_tag="ACHVB7_002094"
     CDS             complement(15467..16090)
                     /gene="tesA"
                     /locus_tag="ACHVB7_002094"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR1914081.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16061..16747
                     /gene="ybbA"
                     /locus_tag="ACHVB7_002095"
     CDS             16061..16747
                     /gene="ybbA"
                     /locus_tag="ACHVB7_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR1914082.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16744..19158
                     /gene="ybbP"
                     /locus_tag="ACHVB7_002096"
     CDS             16744..19158
                     /gene="ybbP"
                     /locus_tag="ACHVB7_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR1914083.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19392..20486)
                     /gene="mnmH"
                     /locus_tag="ACHVB7_002097"
     CDS             complement(19392..20486)
                     /gene="mnmH"
                     /locus_tag="ACHVB7_002097"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR1914084.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20555..21481)
                     /gene="allS"
                     /locus_tag="ACHVB7_002098"
                     /pseudo
     CDS             complement(20555..21481)
                     /gene="allS"
                     /locus_tag="ACHVB7_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5798 g         5144 t
ORIGIN      
        1 atcggcgata agccccagaa ctgccgctcc cagacctccc atgccaaaag caaaaccgaa
       61 aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt agaccagaat
      121 ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc ccgtccagtg
      181 caggctggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat
      241 cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac ctgccgcaac
      301 ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc cgaatttttg
      361 catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt tcgagaaaat
      421 gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg gattgattat
      481 cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac ggctaatttg
      541 cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc
      601 ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa agttgccgcc
      661 gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac gggctacgcg
      721 agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgccg ccagcagaac
      781 tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa agcacatgcc
      841 aattggcaac gaccgcggca tcggatattt atcggtccag tagccgacca ctggttgcag
      901 tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa atgtcagaga
      961 aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca tgtcgttcag
     1021 cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct tggtcgttga
     1081 cgcagccgcg cccgccacag gctggggttg ttcactcatt gccataggaa agtcactttt
     1141 tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta acatacctgt
     1201 atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg gatgcagatt
     1261 tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt caacacgttg
     1321 aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt
     1381 aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata aaacctacaa
     1441 aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg aatatggcga
     1501 atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg ttgcggctga
     1561 aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc ccgagtctga
     1621 cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg acgcgatggc
     1681 gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg aaaagtgggc
     1741 caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc gcctgtttaa
     1801 accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc tgacaaccga
     1861 tgacacagca aaaattggta acccggaata cttcactgat atcgaatttc gtaagcccgc
     1921 cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag acattattat
     1981 cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg caccgggcga
     2041 tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc
     2101 gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg tgccgggtac
     2161 gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg agtggggcaa
     2221 atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg ttaactacca
     2281 gctgattccg gtgaacctga agaagaaagt gaccagggaa gacgggaaaa gcgagcgcgt
     2341 gctgtacacc cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca
     2401 gaacaaaggc aaagcgcagt tggaagtgaa aataggcgaa accaatggtc gtctggaagg
     2461 cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc tggcagccca
     2521 aatggatcgc actggtgccg actttgcggt gatgagtgga ggaggaattc gtgattctat
     2581 cgaagcaggc gatatcagct ataaaaacgt actgaaagtg cagccattcg gcaatgtggt
     2641 ggtgtatgcc gacatgatcg gtaaagaggt gattgattac ctgaccgccg tcgcacaaat
     2701 gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg cgaaagacgg
     2761 caaactcaac gacctgaaaa tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat
     2821 ggcgacatta aacttcaatg ccaccggcgg tgatggctat ccgcgccttg ataacaaacc
     2881 gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata tccagaaaag
     2941 ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc agtaatccga
     3001 aagtgccgga tatttgcatc cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt
     3061 tggcatcgag gatcttcgcc agatcgcctg ccgccagttc gatatccagt ccgcgcttgc
     3121 cgccagaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc gtcggcagac
     3181 gttttttctg ccccaatggg ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg
     3241 ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact tttttaagat
     3301 ccagttgacc tgcgaccggc gtaacggcca ctgccagatg tttcatatca ccgttcactg
     3361 ccaccagtag cgttttgtag acttgatccg gattcaaacc taattttttg acgacttcat
     3421 cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt
     3481 ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac
     3541 gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct aagagtattg
     3601 gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg acggcataat
     3661 aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat ttttttagcg
     3721 caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg cgaccacata
     3781 tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct gatgcatcag
     3841 cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat tttgcggagg
     3901 tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat gccagtgggt
     3961 cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca tggtaatctg
     4021 gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg cctgcaataa
     4081 ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag
     4141 aaccatcgcg atttgccaca gcggttgagt agaaaagagc gacggagaaa tgcccatctc
     4201 ctggctaatg tgctgcagat tttggagttg ctcctcgtta atgcgctctt ccagcgcctc
     4261 gcaagtaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct cgacgatcag
     4321 cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca tatcgtggct
     4381 acccatatga atactgccaa tcagatgaaa atggcgattg ccgggaaggg tgatatcgat
     4441 ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta cccggtacaa
     4501 cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca
     4561 aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt gcatcgctat
     4621 tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg acgacacccg
     4681 gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat gagttagcag
     4741 agttgctgca tgttcatcgt aatagcgtca gtgcactgat caataacaat cgtaaactca
     4801 ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat tttaggctaa
     4861 acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc caggaagaat
     4921 tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg
     4981 tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc acgccgcatc
     5041 cggcatccga gcaccattgc ctgatgcgac gcttacccgt cttatcaggc ctacaaacct
     5101 gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg
     5161 ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct tcggtttaaa
     5221 ccgcagcaac cggttggcgt tactcactac ggtaatcgac gagagcgcca ttgccgctcc
     5281 ggcaactacc gggttaagca gtgttcccgt aaacggccac aaaataccgg cggcgaccgg
     5341 aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt tgcgcagcgt
     5401 tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca tcagggtaat
     5461 cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga catccgcctg
     5521 ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac gtccttcact
     5581 ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa tcacttcatc
     5641 aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg tcaacatcac
     5701 cagacgatat cccgttttat gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg
     5761 atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca caggcgttgc
     5821 tccttgcgat gcctgagcag taatctccgc ttcgatagct ttggtaccaa cctgttgctc
     5881 atttaacagc gcctgattgc ccagcaataa cgcatgacct tcagcttcac cgctcacgcc
     5941 cagcccgcgc aatgtgcgga aaccgttgac ctgcggcagc tgcatatcac ctgctttatc
     6001 gaggatcgcc cgtgccagcg gatggctgga accttgctcc agtgccgccg ccagacgcaa
     6061 tgcctgcgct tcatcaacat cagcaaacgt tttcactgcg acaacctgcg gcttcccttc
     6121 ggtcagcgtc ccagttttat cgaacacgat ggtatcgagt gtactggcgc gttgcagcgc
     6181 gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg aaataatcga
     6241 catcggcgtc gccagcccca gcgcacacgg acaggcgata atcagtaccg tggtggcaat
     6301 caccagggta taaacaattt gcggtgccgg accaaagaag taccagattg ccgcactgac
     6361 cagcgcaatc accaccacca caggtacaaa tacggctgag attttatccg ccagctgacc
     6421 gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccgtg acagcgtggt
     6481 atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca ctgtcccggc
     6541 atggacgctt tcgccttcgc ctttttgctg tgggattggt tcgcccgtca gcatcgcttc
     6601 atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat cgccggtcgt
     6661 caggcgcagt aacatccctg gctgaacttc cgccagcggc acgctttttt caccatctgc
     6721 ggtgaccact cgcgccgtcg gtggggttaa atcgagtaat ttctccagcg ccttagaaga
     6781 acgctgacgt gcgcgcgctt ccagcatatg gccgagatta atcagaccga taatcatcgc
     6841 actggcttca tagtaaagat gccgcgcttc catcgggaac cactgcggcc acaggttgac
     6901 gctcatcgaa tagagccacg ccacacccgt tcccagcgcc accagcgtat ccatcgtcgc
     6961 tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgccgg cgaaaaccat
     7021 cactgccagg gttatcaggc cgataactaa ccacaagctg cggttgtcag cggtgaccat
     7081 catgttatcg ccgatcatcc cccagaccat taccgggata cccacagcca gtgcaacaat
     7141 tgcctgccag cggaagcgct tcatcgtagc tacggcggtt tcttgctggc gctcgcggcg
     7201 tttagcgtca tcttcaatcg cttccgcacc gtagcccgct ttttccaccg cctgcactaa
     7261 atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc
     7321 ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac agctggcgca
     7381 gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg ctgccggaag
     7441 cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca gcggtttagc
     7501 ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga tcagctgttc
     7561 tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgcct gctcaacatc
     7621 cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc
     7681 cagggtcagg tcgatagttt gtgacataaa acactccttt aagacagttt tgactggctg
     7741 tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac caggcgggta
     7801 aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca tttatttctt
     7861 tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga aaatcggcac
     7921 cggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat gttagatgca
     7981 aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact taacggcgga
     8041 caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc ggcagtggct
     8101 atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg ctttgcactg
     8161 gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg
     8221 gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt tatcgcctta
     8281 gagttgcatg gcggcaaacc gctgtcgcca ctggtaaatg ctggcgctat tgccaccacc
     8341 agcctgatta acgctgaaaa tgttgaacaa cgctggcagc gaattttaca tatccaacag
     8401 caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga acaaacaacc
     8461 aacttccata accgggccat tgcctggctg ctgtactccg ccggatatct ctattgtgat
     8521 gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa tactgttgaa
     8581 ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac gcataaacgc
     8641 gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga agggctgtat
     8701 ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc
     8761 ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc accgctggac
     8821 gaagaaggca acagtgttcg cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc
     8881 tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt aacaaacccc
     8941 tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg gggatcttcg
     9001 cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg
     9061 gcggtattgt ggcgatgttt tccggttatg cctatgcgcg tctgggggcg agctatccca
     9121 gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc ttttcgctag
     9181 cgctctcgtt gctgtacctg ttgacgctgg cggtgagcat cgccatggtt gcccgtgctt
     9241 ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac cttattttgc
     9301 tctacgcgtt ggggatcatt gcggtgatga cgctttttaa ctccttaagc aaccatgcgg
     9361 tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta
     9421 ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc cccagctccg
     9481 gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt ggcatgatgg
     9541 cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg tttctggtgg
     9601 cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt agcgatgtat
     9661 cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct tctccgctgc
     9721 tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg gcttcagcca
     9781 ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc gaacgcgaac
     9841 tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaac attattgtcg
     9901 tggtgttgat tatgctgatg acggcggcgc taaatttagg ctcactcgcc agtgttgcca
     9961 gcgccacctt tttgatttgc tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg
    10021 atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg
    10081 ttggctttat ctacagtctg tggtcccagg gtagccgtgc gttgatatgg attattggct
    10141 cactcttact cagccttatt gtggcaatgg tcatgaagcg caataaaacc gtataacatc
    10201 tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc actggcaaaa
    10261 aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg
    10321 atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat tcgcttctat
    10381 gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg cacctacacg
    10441 cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg ctttaacctg
    10501 gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag cgccgacgtc
    10561 aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga gctgcaatcc
    10621 atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag cgccgactgc
    10681 ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt aagacgagac
    10741 ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg tacctgcacc
    10801 gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc
    10861 gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt
    10921 taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag
    10981 ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc aacccagcgg
    11041 caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca acaaataacc
    11101 atttccgccc agcatctcgg ctgccagcag caaaccgccg agactgagcc agaaaatatg
    11161 tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct gtctttcacc
    11221 agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcact
    11281 actttgctgt tactggaggt accgatctgc tgtaacgctt cggtgtattt ctgcgctacg
    11341 aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat tttggtggcg
    11401 cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt
    11461 tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag gatttccgcc
    11521 tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc
    11581 gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg
    11641 actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg tgagttgatg
    11701 ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt acggatgtta
    11761 gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataagc cgcgcgcggc
    11821 gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt atctttcgag
    11881 ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt gcgaccaatg
    11941 cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt atagcgacca
    12001 aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg
    12061 accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac ctcctgttgt
    12121 accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag
    12181 agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga
    12241 acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgggt
    12301 gatgcgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt
    12361 accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc
    12421 ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa gccagaaatc
    12481 taccgccaac aagtctctta ctgcgcccag acaccgacgc tgtttggcga tacggtatac
    12541 gataatctga tctttccctg gcagatccgt aaccggcagc ctgacccagc catttttctc
    12601 gattttctcg aacgcttcgc cttgccggac agcattttga cgaaaaatat cgccgagcta
    12661 tctggtggtg aaaaacaacg catctcactg attcgtaacc tgcaatttat gccgaaggtt
    12721 ttattgctgg atgaaatcac cagtgcgctg gatgaaagta ataaacataa cgttaatgag
    12781 atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac acacgataaa
    12841 gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg agaaatgcag
    12901 gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg cattaatgct
    12961 ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatattct
    13021 ctggagcgtc ggacgagcga taattcagct gattattgtc ggctatgtgc tgaagtatat
    13081 tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc
    13141 ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat
    13201 tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag ggtcgattga
    13261 atttatcccg atgcaggtga tccctatcgc cgggatgatt gccggtaacg ccatggtagc
    13321 ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc aacagattca
    13381 ggagaaactg agtcttggtg cgacgccgaa gcaggcttca gcgatattga ttcgcgacag
    13441 tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt
    13501 accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata
    13561 tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca ttattgcctg
    13621 ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga cgcaattgaa
    13681 gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg
    13741 atcaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg acgccagtgc
    13801 atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc
    13861 ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt cattgcgccc
    13921 aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg gattctccgc
    13981 cacctgctgt tgcaactgtt gaatttccgg cgtatcagcc gcctgcttca gcagttcgat
    14041 ttgcgccacc atcccctggt agcgggtgtc ctgatcctgt aacggaatgg ttttcagcac
    14101 cgcttctgca tcttcagaac ggttcagcgc aatcagcgtt tctgccaaca gcaggccgat
    14161 ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg catcggtgaa
    14221 attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag
    14281 cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga agccatctac
    14341 cggttgcccg ttctggaaca gatacaccgt cggaatcgcg cgcagaccaa actgggcggc
    14401 aatcatctgc tccgcatcgc agtccagctt cgccagaata aattgcccgt tgtactgcgc
    14461 cgcaaggctt tccagaattg gggttaactg caaacagtgc tggctacgtt cagaccaaaa
    14521 atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt
    14581 aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata ttttctttga
    14641 catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca tcacgcgccc
    14701 cggcagcagg cgcttaagca ccattactgc ccaagtcacc agcgttaccg gatagcgcat
    14761 cttcggcttc tcgctaataa aagcatggcg tactttgtcc accaccgctt ccggtcccaa
    14821 cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg tctggttgac
    14881 gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt taattccgct
    14941 gtggcgtagc tccatgcgca gcgcgtctga ccatgcctcc agcgcatatt tactggccgc
    15001 gtaagcgcca cgacccggtg tggagattaa tcccatcacc gatgatgtca tcacaatacg
    15061 cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa
    15121 aaagttggcg gaaaactgct gttccatctg cgcgcggctg atggtggaaa gggggccata
    15181 catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca gggcgatcac
    15241 ttcgtcggct gcgcgatcaa cactttctgg cgaatccaga tcgatcaaca cgccggtaaa
    15301 tcccatgctg ttcatacgct caacatcatc cggtttccgg caacctgcca gcacatgaaa
    15361 accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac atccggtaat
    15421 taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat gagtcatgat
    15481 ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt
    15541 tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct tccataaaaa
    15601 agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg ctaaaggctt
    15661 cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat ggttcagcgt
    15721 tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa
    15781 aaccacgcaa accgtcattg ccgcccagtt caaccagcac ccaacgcggc tgatgctgtt
    15841 tcagcagagc cggaaggcgc gccagtcctt gttgcgaggt gtcgccgctg atgctggcat
    15901 taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag gccgcgctgg
    15961 cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg tccgctgcgg
    16021 cggcacggaa ggttaacagg gccaggaaca ggaagggcaa atgccagcgg aaaacattgt
    16081 tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct ccatcctcac
    16141 cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg agtcgggatc
    16201 gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca gtggcgaagt
    16261 gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc
    16321 gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta acgcgctgga
    16381 aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc
    16441 gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc cggcacagct
    16501 ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc gacctgatgt
    16561 gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata aaattgccga
    16621 cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga cccacgacct
    16681 gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt tgcaggagga
    16741 agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg
    16801 ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg
    16861 gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt
    16921 tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc
    16981 aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc
    17041 aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc ccctggcctg
    17101 aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct taacctgaaa
    17161 acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga agtgattcag
    17221 gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat gaatctggcg
    17281 gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg ttataaattc
    17341 ggcggcaacg agaaccagct cgacggctat gataaatggt tgttacctca gcttaaaccc
    17401 gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc
    17461 tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg
    17521 gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg
    17581 ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg
    17641 acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt gttgatggtg
    17701 ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc gtggctgtgg
    17761 gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata ccgcttgttg
    17821 ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg
    17881 aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg attaatgggt
    17941 ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt
    18001 gtgctgggct ggatgcagct gaatgtactt cgccgcatga cgctgaaatc gctacctctg
    18061 cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca gctttcggca
    18121 ttttcgctct cctttatgct gctggcgctg ttgctggtat tgcgtggcga tctgctcgac
    18181 cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa catcgccaca
    18241 gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc ggaatcattt
    18301 tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga aggtaatgaa
    18361 gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc cgatcataat
    18421 ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga agaggggctg
    18481 gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga tacccaggag
    18541 ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg gcctaatttc
    18601 tattttattt tcccggaagg ggcattagac gggcaaccgc agagctggct taccagtttc
    18661 cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc gaccattagc
    18721 ctgttagata ttggcgcgat tttaaaacag gtcggtcagg tgctggagca ggtaagtcgg
    18781 gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt gctggcacag
    18841 gtgcaggtgg gaatgcgtca gcgtcatcag gagctggtgg tgtggcgcac actcggtgcg
    18901 gggaaaaaac tgctgcgtac cacgttgtgg tgtgagttcg ccatgcttgg gtttgtttct
    18961 ggcctggtag ccgcaattgg tgcggaaacg gcgctggcag tgttgcaggc gaaagtgttt
    19021 gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag cggtgcactg
    19081 ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa ggcgctgttc
    19141 aggcagtttg cggggtgatg acaggtgtta gattgcctga tgcgctacgc ttatcaggcc
    19201 tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata aggcgtttac
    19261 gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa ctgtcatgtc
    19321 ggttttttat atctgtattc tggtgcaacc attctgttct cacgacaatc gccgtttcag
    19381 aataccgccc atcactgcgt cttaacccat tccgccactt ccgcccactc accgcgaaag
    19441 acaacttttt ccgctttttt ctcaagctga tagcgataca tcgggtcgta atattcttta
    19501 agtaacggca ccagccaggc cagatgaccg tcggtgctgt cggtggtgag ttgcgttgtc
    19561 agcgccgtat ccagctgggc agcgagttcg ttatagcgct gtaaccccag ccgacgctta
    19621 atagccgaaa gtccgtgatg caggtattcg caatattcct gccagccctg ttcgtcgccg
    19681 tacgcgtggg taaaatcatg atgcatacgc aagaaatact cttcgttcag gcgctcaaga
    19741 cggatctcaa acggatcttc taccaccgca atcgccgcct gagtcattcg ctcgcgcagg
    19801 cattccggca ggtgattcga accgatcatc cggctttcgt cttccagcac ccacaggcgc
    19861 aaatcctgac gggcgtcggt ttttagcatt tcggcagcca gcaggttttc aaaactcgcc
    19921 tggctaagtt gtggttgtaa ggtgcgacca aacgccgaac cgcgatgacg cgctaaccct
    19981 tccagatcaa caccgttcgg ctgttgctgc actaacagcg ttttaccgct gccggtacaa
    20041 ccgccaatca gcactatcgg tttttgtgcc agttcaatag tcgcctgaat cgcggcttgg
    20101 cgcagtgcct tataaccgcc ttccaccagc ggataatcaa tccccgctgc atacaaccag
    20161 ctttgcacaa tatgtgagcg ctgaccgcca cgggcgcagc agagaatacc ttgcggattt
    20221 tgcaggcacg ctgcccgcca ggcgtccatg cgctgctgac gaatttcacc cgccaccagt
    20281 ttatgcccca gcgccagcgc cgcgtctgag ccttgctgtt tataacaagt gccaacggcg
    20341 gcgcgttcat cgttattcat taacggcagg ttgatagcgg cgggcattgc gccttgctca
    20401 aactcgatag gggcgcgaac atcaataatg ggcgtatcag caatcagcag ggcacgatag
    20461 tcctgttccg tgtgtctctc ttgcatagtt aaaagtgaac ctcaaatcag cttgcgcgct
    20521 attttacgcg ccaacgcgca aggaaacttg atttttaact gcgtgggttg ccgaaaattt
    20581 ctaaaaatcc gctgatttcc ggcctgcgct gggtaaacaa ggtcacaata tcttctaccg
    20641 ctttgccgct gccaaatttg cgccatgcca gactcaatgg cgaaggaggg cgcatcgttg
    20701 ggattacccg gctgaccagt tgttgattat cgatcattga ctggcaaagt gattttggca
    20761 aaaaaccaat gccaacaccc gccagatggg cggcgatttt cgtttccata tcgggaacaa
    20821 taatctcttt ttgccccggc aatcgccagg cgacgcgttt ggttaaggtt cgggcgctgt
    20881 cttcaatatt gaccgccgga aagcgccgca actgcgcttc tgttagcggc tcttcaacgt
    20941 tcgccagcgg atgatccgcc gccatgacaa agcgccattg caccgatcct aagggatcaa
    21001 gactaaaggt atttgccagc gcctcagttc ccgtgacccc gatagccagc gaaaaacctt
    21061 cgtacaatag cgagtcccag acgcccatat agatttgtcg ggagatgtga aactgggtaa
    21121 aggggtaacg ctcattcagc cacgccagca actgggcgac ggcctggggg ttatagagca
    21181 ggttgttgat gacaatattc acctagcgtt ccacgccatc attcacctgt tgcagctcgc
    21241 tgggcatact ttccagccag ctcagccagt ctctggcctg ggaaagtaga tgctcgccag
    21301 ccgctgtcaa cgtcacgctg cgagtcgtgc ggaaaaacag cgccactccg gtattctctt
    21361 ccagaagttt aatgcgataa ctgatcgtcg ccgtggtttt acataatcgt tctgccgctt
    21421 ttgaaaaact tcctgtttca gcaaccgcaa tgaaagtccg caaggtttct ggatcgaaca
    21481 tcttcaggta tcccctttta aatccgcaag ttgcgtgatt ttcttatcct ctgatttatc
    21541 agtattttta catgataacc ctgttcaatt tgtggactaa atctagtttt ggaaaaatat
    21601 tccaactttt gtattgatgt tgttctctta aggttttaga t
//
LOCUS       DAZPJR010000059        21484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPJR010000059 DAZPJR010000000
VERSION     DAZPJR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21484
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.060"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(360..905)
                     /gene="hemG"
                     /locus_tag="ACHVB7_002099"
     CDS             complement(360..905)
                     /gene="hemG"
                     /locus_tag="ACHVB7_002099"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR1914085.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(917..2368)
                     /gene="trkH"
                     /locus_tag="ACHVB7_002100"
     CDS             complement(917..2368)
                     /gene="trkH"
                     /locus_tag="ACHVB7_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR1914086.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2407..3021)
                     /locus_tag="ACHVB7_002101"
     CDS             complement(2407..3021)
                     /locus_tag="ACHVB7_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR1914087.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(3021..4352)
                     /gene="pepQ"
                     /locus_tag="ACHVB7_002102"
     CDS             complement(3021..4352)
                     /gene="pepQ"
                     /locus_tag="ACHVB7_002102"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR1914088.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4542..6731
                     /gene="fadB"
                     /locus_tag="ACHVB7_002103"
     CDS             4542..6731
                     /gene="fadB"
                     /locus_tag="ACHVB7_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR1914089.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6741..7904
                     /gene="fadA"
                     /locus_tag="ACHVB7_002104"
     CDS             6741..7904
                     /gene="fadA"
                     /locus_tag="ACHVB7_002104"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR1914090.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8094..8795)
                     /gene="fre"
                     /locus_tag="ACHVB7_002105"
     CDS             complement(8094..8795)
                     /gene="fre"
                     /locus_tag="ACHVB7_002105"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR1914091.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8842..10335)
                     /gene="ubiD"
                     /locus_tag="ACHVB7_002106"
     CDS             complement(8842..10335)
                     /gene="ubiD"
                     /locus_tag="ACHVB7_002106"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR1914092.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10502..10990
                     /gene="rfaH"
                     /locus_tag="ACHVB7_002107"
     CDS             10502..10990
                     /gene="rfaH"
                     /locus_tag="ACHVB7_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR1914093.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10987..11769)
                     /gene="tatD"
                     /locus_tag="ACHVB7_002108"
     CDS             complement(10987..11769)
                     /gene="tatD"
                     /locus_tag="ACHVB7_002108"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR1914094.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11811..12587)
                     /gene="tatC"
                     /locus_tag="ACHVB7_002109"
     CDS             complement(11811..12587)
                     /gene="tatC"
                     /locus_tag="ACHVB7_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR1914095.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12590..13105)
                     /gene="tatB"
                     /locus_tag="ACHVB7_002110"
     CDS             complement(12590..13105)
                     /gene="tatB"
                     /locus_tag="ACHVB7_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR1914096.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13109..13378)
                     /gene="tatA"
                     /locus_tag="ACHVB7_002111"
     CDS             complement(13109..13378)
                     /gene="tatA"
                     /locus_tag="ACHVB7_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR1914097.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13457..15097)
                     /gene="ubiB"
                     /locus_tag="ACHVB7_002112"
     CDS             complement(13457..15097)
                     /gene="ubiB"
                     /locus_tag="ACHVB7_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR1914098.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15094..15699)
                     /gene="ubiJ"
                     /locus_tag="ACHVB7_002113"
     CDS             complement(15094..15699)
                     /gene="ubiJ"
                     /locus_tag="ACHVB7_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR1914099.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15713..16468)
                     /gene="ubiE"
                     /locus_tag="ACHVB7_002114"
     CDS             complement(15713..16468)
                     /gene="ubiE"
                     /locus_tag="ACHVB7_002114"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR1914100.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16563..17990)
                     /gene="rmuC"
                     /locus_tag="ACHVB7_002115"
     CDS             complement(16563..17990)
                     /gene="rmuC"
                     /locus_tag="ACHVB7_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR1914101.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18131..18892)
                     /gene="udp"
                     /locus_tag="ACHVB7_002116"
     CDS             complement(18131..18892)
                     /gene="udp"
                     /locus_tag="ACHVB7_002116"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR1914102.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19154..19966
                     /locus_tag="ACHVB7_002117"
     CDS             19154..19966
                     /locus_tag="ACHVB7_002117"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR1914103.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20360..21043
                     /gene="tssD"
                     /locus_tag="ACHVB7_002118"
                     /pseudo
     CDS             20360..21043
                     /gene="tssD"
                     /locus_tag="ACHVB7_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            21023..>21484
                     /locus_tag="ACHVB7_002119"
     CDS             21023..>21484
                     /locus_tag="ACHVB7_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914104.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT         5273 a         5744 c         5383 g         5084 t
ORIGIN      
        1 gaattaaact tcgtaatgaa ttacgtgtcc actcttgaga cttggtattc atttttcgtc
       61 ttgcgacgtt aagaatccgt atcttcgagt gcccacacag attgtctgat aaattgttaa
      121 agagcagtgc cgcttcgctt tttctcagcg gcgcggggtg tgcataatac gctttcccgc
      181 tacagagtca agcatttatt tttgctttct ctgtcggaat tctcaggaga accccgctga
      241 cccggcggcg tgtttgccgt tgttccgtgt cagtggtggc gcattatagg gagttattcc
      301 ggcctgacaa gaggaaattt aaaataattt tctgaccgcg cattttttat tctttatgct
      361 tatttcagcg tcggtttgtt ggttaactgg gcgatttctc gggcgaaatt cgccacctgc
      421 tcccaatcgg tatagacaac ttctttgcgc gtatccgttt caccgcctga catcttcata
      481 attagcttga tcataaaacg atcgtaccag cgataacgtg ggtaacgcag cgccccggca
      541 atgaccgcgc agcgatcggg acgccattgc gagttcatca gaaacttccg cgcgtagctg
      601 ttggtctgtg gagtacgctt ctccggtttg cgcgccacca ggtttacgga ataaaaggcg
      661 ctcggcatcg aattcagtcg cgtcgcatgt tttttgacaa attcctggaa cgcagagtgg
      721 tagtgaccat agcgaataga agcaccaatg accacacggt cataattttc ccattgtggt
      781 tcttcaatgc ggtgcacatt ggcgacatcc gtctggatcc ccagttcttt cagttccgaa
      841 gccaggtagg aggcaatctc gcgcgtttgt ccgtcccttg ttgagaaaag aattaatgtt
      901 ttcacgtatt actccatcat tcacgccaga aagtcggggt aaagagcacc agcaatgtaa
      961 agacctcgag acgaccaaac agcatgttgg caatcaggat ccatttagcc accgggttca
     1021 tactggtaaa gttatcagca accacgccaa gccctggccc caggttattc aatgtcgcaa
     1081 caactgaagc aaaggcagaa aagtcatcca cgcccgtggc gataatcgcc agcatactga
     1141 caataaacac caatgcatag gcggagaaaa atccccaaac ggcttcgagg atacgttccg
     1201 gcagtgcgcg attccccagc ttaatgctat agacggcgtt cggatgcacc agtcgtttca
     1261 gctcacggtt cccctgctta aacagcagca ggatgcggat cactttcagg ccaccgcccg
     1321 ttgacccggc gcaaccgccg ataaaagctg aacataaaag cagtaccggc aaaaagagcg
     1381 gccagcgggc aatgctgtca gtcgtaaacc cggcggttgt cgccatcgat accacctgga
     1441 aaaacgcctg gttaattgtc atcagcgccg aactgtagac attatgaaac cacagtacga
     1501 gtgtacaaat aaccaccagc gtaaactgta cgccgataaa catgcgaaat tccggatcgc
     1561 gccaataaac cttcagacta cgcccactta acagtgaaaa gtgcagaccg tagttacagc
     1621 cggagatcag caggaagata gcaatgatag tgttaatagt cgggctgtcg aaataaccga
     1681 tactggcatc atgtgtcgag aagccgccaa tagcgatagt cgcaaagcta tggccgatgg
     1741 catcaaaggc atccattccg gcaaaccaca acgccagcgc acaggcgacg gtcagcaaga
     1801 cataaatcaa ccacagggtt ttcgccgttt ccgcaattcg cgggcgcatt ttgttatctt
     1861 tcagcgggcc gggcatttct gcgcgataga gctgcatccc acccacgccg aggataggca
     1921 gtatcgcaac cgctaacacg atgatcccca tcccgccaaa ccattgcagc atctggcgat
     1981 aaaagaggat ggcatgaggg agcgaatcca gccccaccag cgtagtggcc ccggtggtgg
     2041 tcaggccaga gaaagattca aaaaacgcat cggtaatcgt gaggttcggg ctttccgaga
     2101 agataaaagg gagcgcaccg acactaccca gcacggtcca gaacagcacc actatcagaa
     2161 acccctcacg ggatttcagt tcgcccttct ctttacggtt cggccaccac agcatagaac
     2221 caatggcgag ggcgacaaaa aaggtctggg taaaagcgcg gcccgctcca tcccggtaga
     2281 tgagtgctac cagcccaggg ataatcatgg tccctgaaaa taagatgacc agtagtccaa
     2341 cgattcgggt aatggcgcga aaatgcatct ctgccgcttc cttagttctg caaaacgaag
     2401 tggggattat tcttcaatcg ctaacaattg caatgaacca cggctaaaat ccgccagctt
     2461 tgcggaaaat tcagccactt tcgccgccgg aagcgccacc cgcagcagaa cgaatgcctg
     2521 ataatcactg ttgataattt tgccgtcaca ctgccccagc aacgcttcaa tgccggttaa
     2581 ctgatgatat tcacattgca aagtatattc ggttaatggc gtcttgcgtt gggtcgttag
     2641 ctggcgcagc gcctgattca cgccgccgcc atacgctttc actaacccac cggtgccaag
     2701 caatatgccg ccgtagtagc gcactaccac agaggtaatt tccccgacgc cgctgcccat
     2761 tagctgggcg agcatcggtt tacctgccgt tcctgccggc tccccgtcgt cagagaaacc
     2821 cagctgttga gaatcatccg gcgcacccgc cacccacgcc acgcaatggt ggcgggcatc
     2881 ggggtgttct gcccgcaccg attcaacaaa cgctttcgcc gcctcaacgc catcggtatg
     2941 cgccaacatc gttatgaagc ggcttttttt gatctcttca acgaccgtga ccggtgccgc
     3001 aggaattaac cagctttcca tcacgccagt ttcagatccc gggtcatgtt ttccacgttg
     3061 ttttcgtgga tcaccacgtt gtcttcgata cgaatgccgc cgaacggttt cagtgcttca
     3121 attttctgcc agttgaagtg cttgctgaac tgcccttcac gccacggtgc cagtagcgat
     3181 tcaatgaagt agatacccgg ttcgatggtt aacaccatac ccggctggag aatacgggtg
     3241 cagcgcaggt acggatattt tgccggtgcc gcgaggtgtg taccgctatc atcctgcata
     3301 aaaccggcta cgtcatgcac ctgcaggccc agcggatgac cgataccatg cggcataaac
     3361 ggtccggtga gatcgttttc gaccatcgct tcttcactca tatcggtgat gatttgatgt
     3421 ttacgcagca gtttggcgat gcgctgatgg aactggatgt ggtaatccac atagctaacg
     3481 cctgctttca tggtggcgat cagcgccagt tgttcatcat ttacgtcttt caccagctgt
     3541 gcgtagtcgt tgtcgctttt tgccgaccag gtacgagtca ggtcagccgc atagccgtta
     3601 tattcggccc cggcatccag caggaagctg cgcatctctt ccggtgcctg atgatccagt
     3661 ttggtgtaat gcagcaccga agcgtgttcg ttaagcgcca caatgttgct gtaaggtacg
     3721 tcggtatcac gatgaccggt cgcggtcaga taggcgatgt tgatatcgaa ctcgctcatg
     3781 ccagaacgga acgcttcttc tgccgcacga tgaccgttga ccgccatttt ctgcgcttca
     3841 cgcatacagg ccagttcgta ctcggttttg aaggagcggt agtaatgcag gtagtcgatc
     3901 acccctttcg ggttgatgtt gctggcctca atacccagtt gcagcgcacg ttccggcacc
     3961 ggaccgatat aaccgatatt gccgcgcgca gcgggcagca ggctaccaat gccatcggct
     4021 ttcggcagtg cgatcacttc cacatcttca gtccagaagg aggtcggcag cggttcgacg
     4081 ttgtgccagt aatcaaccgg cagatagaac cacagtttcg gcttgttcac gccatccacc
     4141 agcaaccagc agtttggcac ctgagttacc ggcacccacg ctttgaattg cgggttcact
     4201 ttaaacggat agggatgatc gtcgagaaaa acgttgaaca gctcgccgga gtgaataagt
     4261 aatgcatcca gcttgaagcg cgccagcgca tcgcgagtcc gttcttgtaa ggtagctata
     4321 tgatttttat agagcgaggc cagtgattcc attttttacc cttctgtttt tttgacctta
     4381 agtctccgca tcttagcaca tcgttcatcc agagcgtgat ttctgccgag cgtgatcaga
     4441 tcggcatttc tttaatcttt tatttgcata tttttaacac aaaatacaca cttcgatgca
     4501 tctggtacga ccagatcacc ttgcggattc aggagactga catgctttac aaaggcgaca
     4561 ccctgtacct tgactggctg gaagatggca ttgccgaact ggtatttgat gccccaggtt
     4621 cagttaataa actcgacact gcgaccgtcg ccagcctcgg cgaggccatc ggcgtgctgg
     4681 aacagcaatc agatctaaaa gggctgctgc tgcgttcgaa caaagcagcc tttatcgtcg
     4741 gtgctgatat caccgaattt ttgtccctgt tcctcgttcc tgaagaacag ttaagtcagt
     4801 ggctgcactt tgccaatagc gtgtttaatc gcctggaaga tctgccggtg ccgaccattg
     4861 ctgccgtcaa cggctatgcg ctgggtggtg gctgcgaatg cgtgctggcg accgattatc
     4921 gtctggcgac gccggatctg cgcatcggtc tgccggaaac caaactgggc atcatgccgg
     4981 gctttggcgg ttctgtacgt atgccacgta tgctgggcgc tgacagtgcg ctggaaatca
     5041 ttgccgccgg taaagatgtc ggcgcggatc aggcgctgaa aatcggtctg gtggatgggg
     5101 tagtcaaagc agaaaaactg gttgaaggcg caaaggcggt tttacgccag gccattaacg
     5161 gcgacctcga ctggaaagcg aaacgccagc cgaagctgga accgctaaaa ctgagcaaga
     5221 ttgaagccac catgagcttc accatcgcta aaggaatggt cgcgcaaacg gcggggaaac
     5281 attatccggc ccccatcacc gcagtaaaaa ccattgaagc tgcggcccgt tttggtcgtg
     5341 aagaagcctt aaatctggaa aacaaaagtt ttgtcccgct ggcgcatacc aacgaagctc
     5401 gcgcactggt cggcattttc cttaacgatc aatatgtaaa aggcaaagcg aagaaactca
     5461 ccaaagacgt tgaaaccccg aaacaggctg ctgtgctggg cgcaggcatc atgggtggcg
     5521 gtatcgctta ccagtccgcg tggaaaggcg tgccggttgt catgaaagat atcaacgaca
     5581 agtcgttaac cctcggcatg accgaagccg cgaaactgct gaacaagcag cttgagcgcg
     5641 gcaagatcga tggtctgaaa ctggctggcg tgatctccac gatccaccca acgctcgact
     5701 acgccggatt tgaccgcgtg gatattgtgg tagaagcggt tgttgaaaac ccgaaagtga
     5761 aaaaagccgt actggcagaa accgaacaaa aagtacgcca ggataccgtg ctggcgtcta
     5821 acacttcaac cattcctatc agcgaactgg ccaacgcgct ggaacgcccg gaaaacttct
     5881 gcgggatgca cttctttaac ccggtccacc gaatgccgtt ggtagaaatt attcgcggcg
     5941 agaaaagctc cgacgaaacc atcgcgaaag ttgtcgcctg ggcgagcaag atgggcaaga
     6001 cgccgattgt ggttaacgac tgccccggct tctttgttaa ccgcgtgctg ttcccgtatt
     6061 tcgccggttt cagccagctg ctgcgcgacg gcgcggattt ccgcaagatc gacaaagtga
     6121 tggaaaaaca gtttggctgg ccgatgggcc cggcatatct gctggacgtt gtgggcattg
     6181 ataccgcgca tcacgctcag gctgtcatgg cagcaggctt cccgcagcgg atgcaaaaag
     6241 attaccgcga tgccatcgac gcgctgtttg atgccaaccg ctttggtcag aagaacggcc
     6301 tcggtttctg gcgttataaa gaagacagca aaggtaagcc gaagaaagaa gaagacgccg
     6361 ccgttgaaga cctgctggca gaagtgagcc agccgaagcg cgatttcagc gaagaagaga
     6421 ttatcgcccg catgatgatc ccgatggtca acgaagtggt gcgctgtctg gaagagggca
     6481 ttatcgccac tccggcggaa gcggatatgg cgctggtcta cggcctgggc ttccctccgt
     6541 tccacggcgg cgcgttccgc tggctggaca ccctcggtag cgcaaaatac ctcgatatgg
     6601 cacagcaata tcagcacctc ggcccgctgt atgaagtgcc ggaaggtctg cgtaataaag
     6661 cgcgtcataa cgaaccgtac tatcctccgg ttgagccagc ccgtccggtt ggcgacctga
     6721 aaacggctta aggagtcaca atggaacagg ttgtcattgt cgatgcaatt cgcaccccga
     6781 tgggccgttc gaagggcggt gcttttcgta acgtgcgtgc agaagatctc tccgctcatt
     6841 taatgcgtag cctgctggcg cgtaacccgg cgctggaagc ggcggccctc gacgatattt
     6901 actggggttg tgtgcagcag acgctggagc agggttttaa tatcgcccgt aacgcggcgc
     6961 tgctggcaga agtgccgcac tctgtcccgg cggttaccgt caatcgcttg tgtggttcat
     7021 ccatgcaggc actgcatgac gcagcacgaa tgatcatgac tggcgatgcg caggcaggtc
     7081 tggttggcgg cgtggagcat atgggccatg tgccgatgag tcacggcgtc gattttcacc
     7141 ccggcctgag ccgcaatgtc gccaaagcgg cgggcatgat gggcttaacg gcagaaatgc
     7201 tggcgcgtat gcacggtatc agccgtgaaa tgcaggatgc ctttgccgcg cggtcacacg
     7261 cccgcgcctg ggccgccacg cagtcgggcg catttaaaaa tgaaatcatc ccgaccggtg
     7321 gtcacgatgc cgacggcgtc ctgaagcagt ttaattacga cgaagtgatt cgcccggaaa
     7381 ccaccgtgga agccctcgcc acgctgcgtc cggcgtttga tccagtaagc ggtacggtaa
     7441 cggcgggtac atcttctgca ctttctgatg gtgccgcggc catgctggtg atgagtgaaa
     7501 gccgcgcccg tgaattaggt cttaagccgc gcgcccgtgt gcgttcgatg gcggtcgttg
     7561 gttgtgatcc gtcgattatg ggttacggcc cggttcctgc ctcgaagctg gcgctgaaaa
     7621 aagcggggct ttctgccagc gatatcggcg tgtttgagat gaacgaagcc tttgccgcgc
     7681 agatcctgcc gtgcattaaa gatctcgggc taatggagca gattgacgag aagatcaacc
     7741 tcaatggtgg tgcgatcgcg ctgggtcatc cgctgggttg ttccggtgcg cgtatcagca
     7801 ccacgttgct gaatctgatg gaacacaaag acgttcagtt tggtctggcg acgatgtgta
     7861 tcggtctggg tcagggtatt gcgacggtgt ttgagcgggt ttaaccactt gccggatgcg
     7921 gcgtgaacgc cttatccggt ctaccgatcc tgcaccaatg taggcctgat aagacgcgcc
     7981 agcgtcgcat caggcatcgt gcaccgattg ccggatgcga catagatcta aatgccactg
     8041 aataaagttt tagttgccgt tcttcccgcc tgtcaggggc gggtttttat atctcagata
     8101 aatgcaaacg catcgccaaa cagacgatct tcccgcgcat tacgctcact gcaaaacagg
     8161 tcacgagcaa ttttcgccat ctcaaaacgt ccggcaatat agatatcatg ctctgccagc
     8221 gtaccatgat cctgcaagac cgccgttaac acggtgccag tacgcccacg ccagcccgct
     8281 tccggttgtt caaccaccgg caccacttgc agaccaggat gcttcagcga aagcgcctca
     8341 agctcgcaga gatcatacag atgttgctct tcacgcccac cccagtaaat ggtgatatcg
     8401 cgattgggat tacgcgccaa cgccgtcaat aaaatcgagc gcgcatagga gaacccggtg
     8461 ccgcccgcaa tcaaaatcat cggacgctct tcatcatcgc gcagccacgc ttctccgtgg
     8521 ggaatatcga ccacgatttg atgatctttg aggatgcggt ccatgactgc tttcgcgtaa
     8581 aggttgattt cagaagcgcc aatatgcagc tcgataaacc ctttttcatc cggcgtcgaa
     8641 gccattgaaa acggacgttt gtcgcgctca tccatcacta ccatcaaata ctgaccagca
     8701 cgaaaagaaa aggccgcgtc tggtacgatt cggacacggt atacagtatc cgtgatagct
     8761 tctaccgagg tcactttaca gcttaaggtt gtcatgcgct ttctctgtcg gatcaaataa
     8821 atagggcaaa actaacgcgc atcaggcgct tttaccgttg ttaaaaatag ccagttcatc
     8881 ccagatggcg tcaatatgcg cgacgacatc aggatctttt ttgatgggac gtccccattc
     8941 acgctgggtt tcccccggcc atttattcgt ggcatccagc cccatttttg aacccagccc
     9001 ggagacaggc gaggcaaaat ccagataatc aataggcgta ttttctacca gaacagtatc
     9061 ccgcgccggg tccatacggg tggtaatcgc ccaaatcaca tcgttccagt cgcgtgcgtt
     9121 gacgtcatca tcgcaaacga tcacaaattt agtgtacata aactggcgta agaacgacca
     9181 gacgcccatc atgacgcgct tcgcgtgtcc ggcgtactgt tttttgattg tcactaccgc
     9241 cagacgataa gaacagcctt ccggtggcag gtaaaaatcg acaatttccg ggaactgttt
     9301 ttgcagaatc ggcacaaaca cttcgttcag tgcgacaccc agcaccgcgg gttcatccgg
     9361 cggacgacca gtataggtgg agtggtaaat cgcatcttca cgctgggtaa tatgcgtcac
     9421 ggtaaatacc gggaaactat cgacttcgtt atagtaaccg gtgtggtcgc catacggccc
     9481 ttccggcgca gtttcgcctt gttcgatata cccttccagc acaatctccg cactggcggg
     9541 cacttcaagg tcattggaga tacacttcac cacttcggtc ttggtaccgc gcagcaatcc
     9601 ggcaaacgca tactctgaaa gtgtatccgg aacgggggtg actgcaccga gaatcgtggc
     9661 gggatcggca cccagcgcca cagaaaccgg gaaacgttcg cccggatgcg ccgcacacca
     9721 ctcctgataa tccagcgcgc cgccgcgatg cgacagccag cgcataatca gtttgttttt
     9781 accaatcagc tgctggcgat aaatgcccag attctgccgc tctttatgcg ggccgcgcgt
     9841 tacggtcagc ccccaggtaa tcagcggcgc ggcatcttcc ggccagcagg tcataatggg
     9901 aatgcgattg agatcgacgt catcgccaga gacgattttt tgttggcagg gcgcaccacg
     9961 cagccgcttt gtcggcatgt tcaatacttg cttaaactgc ggcagtttat caaacaggtc
    10021 gcggaaacct tttggcggct cgggctcttt cagaaacgcc aataatttac caacttcacg
    10081 cagcgccgaa acatcttcct gccccatgcc catcgccacg cgctttggcg taccgaacag
    10141 gttgcacagc accggcattg agtagccttt agggttttcg aacaacagcg caggcccacc
    10201 ggcacgcaaa gtgcggtcag caatttcagt gatttccaga tgcggatcca ccgggagcgt
    10261 gatacgtttt agctcaccct gctgttcaag cagcgtcaag aagtcgcgta aatcgttata
    10321 tttcatggcg tccattgtag cctcttaatc tacgcccatt atacggcgtt catctttgcg
    10381 atgctgtaaa tttgttaaat tagcgtgaac tctgacggta taacgcaaac cggggaatat
    10441 aattaactta gcgtaaagct tttgctatcc ttgcgccccg attaaacgga taagagtcat
    10501 tatgcaatct tggtatttac tgtactgcaa gcgcgggcaa cttcaacgtg cccaggaaca
    10561 ccttgagaga caggctgtga attgcctggc accgatgatc accctggaaa aaatcgtgcg
    10621 tggaaaacgt actgcagtca gtgagccatt gtttcccaac tacctgtttg tcgaatttga
    10681 tccagaagtg attcatacca cgactatcaa cgcgacccgc ggtgtcagcc acttcgtgcg
    10741 ctttggcgcg tcgctagcga tagtcccatc ggcggtgatt catcagctat cggtatataa
    10801 accgaaagac attgtcgatc cggcaacccc ttatccggga gataaggtga ttattaccga
    10861 aggcgcgttc gaaggctttc aggccatttt caccgaaccc gatggtgagg ctcgctccat
    10921 gctattgctt aatcttatta ataaagagat taagcacagt gtgaagaaca ccgagttccg
    10981 caaactctaa aacgcaatcc caaaccgtgt tttgacatta gcatccgtgg tggcagccag
    11041 ccatgcggca tcttctccac gccagtgcgc aatacgttgc aaaatatggg gcagatgggc
    11101 tggctcgttg cgccgggatg atggctttgg cgtgagatcg cgagggagca gatacggcgc
    11161 atcagtttcg atcagtaatt tttccgccgg aatcaacggc aaaagttccc gcagctccag
    11221 tccgcgtcgt tcatcgtaaa cccaaccggt aatgccgata taaattccat gcgccacgca
    11281 cgcctgcatc tcttcgcgtg tgccggtaaa gcaatgaaga accgcaccag gcagtttatc
    11341 cagccacggc tccagcaatg tcataaaccg ctcgtgggca tcgcgacagt gcataaatac
    11401 cggcatgttt aaatctgcgg caatgcgtag ctgggcaaca aaagcgcgtt cctgctcttc
    11461 cggcgtcgaa aagttgcggt taaagtcgag accacattca ccaatcgcca ccacttctgg
    11521 ctgcgcggcc agctcaataa tcgcttcttc agtcgcagct tgccactggc tgctgtcgtg
    11581 aggatgtacg cccgccgttg accaacagga cgaatactga cgcgccagct tttgcgcctg
    11641 ctggctttca cgcaggttag tgccggtgat gagtagccca ttaactcccg cgtcaaaagc
    11701 gcgcgctaca acatcatcac ggtctttcgc aaattgcgaa ctggtcaaat taacgccgat
    11761 atcaaacatc ctgtactcca tatgacaacc gccctgacgg gcggttgaat ttattcttca
    11821 gttttttcgc tttctgcttc agcgtcgttt tcctcttccc gatttcgccc tttaccaacg
    11881 taaaagcgtg agaagaagac accgatttca aacagacagt acatcgggat cgccaatagc
    11941 gtttgcgaga agacatccgg cggcgtcagc aacatcccga caacgaatgc accaaccagc
    12001 acatacgggc gttttttgcg taagtcttct ggcgaggtaa tccccatcca gcacagcagc
    12061 acaattgcca ccggcacttc aaaggagaca ccaaacgcca taaacagcgc cataacgaag
    12121 cttaaatagc tggcgatgtc ggtggatacc tgcacccctt ccggcgcggt attggcaagg
    12181 aagccaaatg ccagcggaaa gaccacaaag taggcgaatg ccatgccgat ataaaacagc
    12241 agagagctgg aaaccagcag cggcaccacc aggcgacgtt catgcttata cagcgctggg
    12301 gcgataaatg cccacacctg atagagaatc accggcgctg acagaatcag cgacaccata
    12361 aaggtcagct tgatcggcgt aaagaacggc gaggccacgt cggtggcgat catcgttgaa
    12421 ccttgcggca actgcttgat caatggcgcg gataccaggt gatagatgtc attggcgaaa
    12481 tagaccagac acaggaatat cacgatcacc gcgataatgc agttcagcag acgcttacgc
    12541 agctcaatta gatgcgtgat aagcggttga gtatcttcta cagacatgtt tacggtttat
    12601 cactcgacga aggggaaggt gcagcggttt tcggttcagc gtccgcagca ggttttacca
    12661 ccggctctgg cgtggtttct ggcttctgtt ccggcgaact ggcctgcgtt tgtgcagcgg
    12721 caggcgttac gccctcatgc gcagcttcat tatctttcac caccgggtta tggatggtgt
    12781 gcgcttcatc gctcgccttt tcaggatcgt ttgcaacgta ggaacgcttc atcgactccg
    12841 cggcctggcg tagttcatcc atcgacgctt tcagttcggg cgtcaggtta gtgaggctcg
    12901 ccttttcaac ctttttcaga ctgtcctgaa actcctggag ttttaactcc tgggtcagtt
    12961 cgttctgcac cgttgtcgcc agtgaacgca acgcgcgaat ccagcccgct accgttttta
    13021 ccgccacagg cagtcgttgc ggccccagaa cgacgaggcc gatgatgaac accaatagca
    13081 gttcgctaaa accgatatca aacacggatt acacctgctc tttatcgtgg cgcttcgcgt
    13141 cttctgtttt agcctgttcc tgattcgtat ccgcctgctt atcggcgata gttttcgcag
    13201 taaaatcagc atcctgactg gttttatcct gctttggttc atcatcgctc attgcttttt
    13261 taaagccttt gatcgacgca ccaagatcgg aaccgatgga gccgagcttt ttggtgccaa
    13321 aaagcagtac aacgatgacg gcaataatca ataactgcca aatactgata ccacccatac
    13381 atgttcctct gtggtagatg atgattaaac aaggccgcat tatacgttac acgacccgcc
    13441 ttgagcgatg aaaaaatcag cgtgttttgc gccaaccgac aaaccaggcg atcagaccac
    13501 ctgccattaa ccagccgggc atcagccccc attcaggtcg gctgaccaac aagaatgtgc
    13561 cacttaatac taacgtagcg ccaattccga gaaaataacg cgattgtccc tgacgtacat
    13621 gatttgactg aagctcgcgg gcaatcttat caacactgtg ctgtaaatac ttgccctggc
    13681 gcaaactgtc gtaaaccaat tcaggcagtt ctggcatttt ttcgacccag aacggcgctt
    13741 tttctttaaa tgctctcacc agggcaggaa taccgacctg atctttaatc cacgactcca
    13801 ggaaaggctt cgctgttttc cataaatcga gctgcggata aagctggcgt cctacccctt
    13861 cgacatagag cagggttttc tggagtaaca ccagttgcgg ctgcacttcc atattgaagc
    13921 gacgcgccgt attaaacaga tttaacagta catgtccaaa cgaaatttcg gccagcggtt
    13981 tctcaaagat aggttcacag accgtacgaa tggcaaattc gaactcttca acgttggtat
    14041 ctggtggcac ccagccagaa tcgacgtgta gctctgccac tttgcgatag tcgcgattaa
    14101 agaaggcgat aaagttttct gccagatagc gtttatcttc tttgtttagc gagccaacaa
    14161 tcccgcaatc aatgccgata tatttcgggt tttccgggtg ttcatagctt acgaaaatgt
    14221 tgccagggtg catatcggca tggaaaaagc tgtcgcgaaa gacctgagtg aagaacacct
    14281 gcacgccacg ttccgccagc aatttcatgt tggtgccatt tttctccagc gtcgcaacat
    14341 cagacaccgg aatgccgtaa atgcgctcca tcaccatcat cccttcacta caatagtcag
    14401 ggtaaacttc cgggatgtag agcatcgggc tgtcttcaaa atttcgccga agctgaatgg
    14461 cgttggcaga ttcccgcagc aaattcagtt catcaatcaa ggtcttttcg tactcgcgca
    14521 ccacttcggt tgggcgcaga cggcgaccat ccggcagcaa acgcggcacc cagcgagcca
    14581 gacggtagat aagtttcaga tccgctttaa taaccggcaa aatatccggg cggatgactt
    14641 taatcaccac ctctttacca ttcgatttca atcgcgcggt atgaacctgg gcgatagaag
    14701 cagaagccag tggcttgatt tcaaaatcgt caaaccacgc ttctaccggc aagccgccca
    14761 ttgcagcttc aatctgctgc ttcgccagct tgccatcaaa cggagcaact ttgtcctgca
    14821 ataacgccag ctgatcggca atatgcggtg gaaaaagatc gcggcgggtt gataacattt
    14881 gcccgaactt gatccaaacc ggccccagtt cttgcagggc cagtcgtagt cgctcaccta
    14941 aaagtttgtc tttatgccga tttggcatcc agaataatga gtatcgccat agccgtagcg
    15001 gcagggtgat acgcattttg gggatcagtt catcaagtcc gtagcttaaa aaagtgcgaa
    15061 tgatgaaata taggcgccgt acttcacctg gcgtcattta gcctccagtt tttccagccg
    15121 tttggtcagg gcatcaacag cacgctcgac ggcagccgtc tcttccgcaa accaggccac
    15181 ttcaagcgga ccgggtgcca tacgccactc ttcagtaatg gcttccgcca cataacgttg
    15241 ctggcgctta atgccgtgat gcaggaactt tgcgcctccg cgcatggctt tgctgattcc
    15301 ttcagcggcg atatcaccgg tataaggggc cagcagttcc gcagggtcga actctgccag
    15361 atctgccagc gcaacgaagt tttgcaccac ctgaatatcg ccctgcactt ccagctcacc
    15421 actgcgaatc agtgcggtaa gctgctggcg atcgcgaagt ttcggcaaca cactggcgta
    15481 ggcgataacg gtgcagtcag catcgcctgc ccattcgccc agtacatcaa cctggcgttc
    15541 gctgaacacc agaatcaatg acgtcgaaaa gccttttacc tccacgcgca atactttacc
    15601 cagcagacgc gagcgggctg ttttcagcgc gggtgagcga tacaggaagg tgttgagcag
    15661 actttcaatt cctgccgtca ctaaaggttt aaaaggcatt tccggtctcc tgtcagaact
    15721 tataaccacg atgcagcgcc acaacccctg ccgtcagatt gtagtagtcg acactttcga
    15781 atccggcatc ctgcatcatg gctttcaggg tatcctgatc gggatgcata cggatggatt
    15841 ctgccagata acggtagctg tcggcgtcgt tcgcgaccag tgagccaata cgcggcagca
    15901 catggaagga gtatgcatca taggctttgc tcagcggctc gataattggc ttcgagaact
    15961 caagcaccag caggcggccg ccgggtttca gcacgcgata cattgaacgc agtgctttat
    16021 ctttgtcggt gacgttacgc agaccaaacg aaatggtgat gcaatcaaag gtgttatccg
    16081 ggaacggcag cgcctcagcg ttcgcctgaa catactcaac gttgccaatc acaccgatat
    16141 tacgcagctt ctcgcgtccc attttgagca tggattcatt gatatcagca aggaccactt
    16201 tgccagtttc tccgaccagg cgggagaatt tcgctgtcag gtcgccggtg ccaccagcca
    16261 gatccagcac ggtctgccca cggcgtacgc cgctgcaatc aatcgtgaat cgcttccaca
    16321 aacgatgaat accaaatgac atcaaatcat tcatgacatc gtattttgat gccacggaat
    16381 ggaaaacgtg ggcgaccata tccgcttttt gttccttcgc gacggtctga aaaccaaagt
    16441 gcgtcgtttc ttgtgactta tccaccatct caatgcctgc tcatcaaaaa attgttccag
    16501 aagtgtaaca gattgggcgt cgatgcccta gatttctacc cggcttaact acccctaatg
    16561 ggctagcgcg actgctgatt atattcatca tcgcgttgat aagcttcatc attcggctgc
    16621 tccggaaccg accgaagtcg atactcttca ttctggctca ccgcctgttc agccaaatcc
    16681 ggattaatct cgcgtttaat ttctactcct aaaccgcgaa acgcttctgc ctgcgccagc
    16741 acatttccgc gacctgaaga gagttttttc attgcctggc ggtagttatc ctgcgctttg
    16801 tcgagacttt gaccaatcgc ggacatgtca tcgatgaaca aacgcatctt gtcgtacagc
    16861 ttgctggcac gatcggcgat ttgctgggcg ttgcggcttt gatgctcata acgccacagg
    16921 ttggcaatag tacgcaacgc caccagcagc gtagtcgggc taaccagcat gatattgttt
    16981 ttcaacgctt cggtgatcag ctccggctgg cggtcaagcg ccagtaaaaa agcgggttca
    17041 acgggaataa acatcagcac gtaatccaga gttcgcagcc ccggcagctg ttgataatct
    17101 ttgcgtccca gcaaacggat atggttacgc accgacgcga tatgttcctg tagcgcgctt
    17161 tcgcgggtgt agtcgtcttc ggcgttaaaa tagcgttcat aggcgaccag cgtcattttg
    17221 gcgtcgatca ccacatcttt tccctgcggc agccgcacga tgacatccgg ctgcatccgc
    17281 gagcgggcgt cattttcgat gctgacctgg gtttcatatt catacccttc acgcagaccg
    17341 gaagcctcca gcacccgcgt caatactacc tcgccccagt tgccctgggt tttattgtcg
    17401 cctttcagcg cgcgcgtcag gttgatcgct tcctgggcca tttgcgcgtt gagttgctgg
    17461 agattgcgaa tttcgtgggt cagggtatgg cgttcttgtg cttctttacc gaagctgtcc
    17521 tgaacctgac ggcggaaacc gtccagttgt tcacgtagcg gcgacaacag gctgttcaga
    17581 ctctgacggt tttgctcatc aacccggcga ttgctgtgct caaaaatacg gttggcgagg
    17641 ttttcaaact gctcactgag gcgctgctcg ctgttaatca tctggcgaat tttatcgtca
    17701 gcatgttgct gtgcggcttc catccgcgtg gttacttcac gcagatcggc ctccagagag
    17761 gtgttaatac tttgcaggct gcgcacttcg ttattgagta actcgcactc tgcacgccag
    17821 tgctcgcttt gggtaatttg ttgttttgcc gcgcttaact ccgcgaccat ctcttcacgt
    17881 tcagctaatt gctcggcttt ttgctgcgca tgttgataac tggcaaacag ccagccaatt
    17941 gccacaccca ccaacgcaat aactgcgtaa accatgattg agaaatccac aacgcctcct
    18001 gacgcaacaa cttacccgca caaaatagaa ttgtgctgta caaacgtcca gttggaaagg
    18061 catttcctgc gaggcgctac gcaaaaatac aaaaggccga acgcgtcggc cttcagacag
    18121 gagaagagaa ttacagcaga cgacgcgccg cttccaccac gattttcacc gcttggcttt
    18181 cagtttgttt catcgtctca gcattcggga tctcttgctg ggtgcggtta acgataacac
    18241 ccgctaccat accggcacgc aggccctgac ttgcacacat ggtcagcagg gttgcagatt
    18301 ccatttcata gttcattatg cccatcgcct gccactcttc catagaacct ttaaagtgac
    18361 gaactacgcg accagagtaa gtatcgtaac gttcctgacc tgggtagaag gtatcagaag
    18421 aagctgtcac gccaacgtga gttgtcgcgc caatggattt cgcagcttca accagcgcag
    18481 tcgtacattc gaaatcagcg acagccggga attccagcgg tgcgaagtgc aggctcgcgc
    18541 catccagacg gacagacgcc gtggtaacca ggacatcacc cacattaata tgcggctgaa
    18601 tagcgcccgt tgtaccgata cgcaggaagg tgcgaatgcc cagctgtgcc agctcttcaa
    18661 cagcaataga ggtagacggg ccgccgatac cggtagagca gacgataaca ggtttaccat
    18721 ccagctctgc acgccaggta gtgaattcgc ggtgagatgc cagcttaacc ggcttatcca
    18781 tcagcgcggc gatcttttcc acacgatccg ggtcgccagg gacgatggca agcgtagccc
    18841 cttgtaaatc gtttttagtg aggccgagat gaaaaacatc agacttggac atatacaact
    18901 cctctgtgaa tcggtttagt cagaagaagc aaaaagacac tttaccgaag ggtttaacat
    18961 tttttcgtga tactcatcac catgacgcaa atgcgttgca taaatttacc caccaaaagt
    19021 gattcaaatc acataaatca ctcttcattc tgcaggactg cataaaagat gagccgattc
    19081 aggcaatgtg atttgttgca tgctgactat agttaacatt gcgcaacatt tttataaggg
    19141 tacggagaat accatggcaa caacacaaca atctggattt gcacctgctg catcgcctct
    19201 cgcttcgacc atcgttcaga ccctggacga cgcgattgtg gcgggcttca cctctatccc
    19261 ttcacaaggg gataacatgc ctgcttacca tgccagacca aagcaaagcg atggcccact
    19321 gccagtggtc attgtagtgc aggaaatttt tggcgtgcat gaacatatcc gcgacatttg
    19381 tcgccgtctg gcgctggagg ggtatctggc tatcgcacct gaactttact tccgcgaagg
    19441 cgatccgaat gattttgccg atatccccac gttgcttagc ggtctggtag caaaagtgcc
    19501 tgactcgcag gtgctggccg atctcgatca tgccgccagt tgggcttccc gcaacggcgg
    19561 cgatgttcat cgtttaatga tcaccggatt ctgctggggt ggacgtatca cctggctgta
    19621 tgccgcgcat aatccacagc taaaagctgc agtggcgtgg tacggcaaac tgacgggcga
    19681 caagtcgctg aattcaccga aacaacctgt tgatatcgca accgatctta acgcgccgat
    19741 tctcggctta tatggcggtc aggataacag cattccgcag gagagcgttg aaaccatgcg
    19801 ccaggcgctg cgggctgcta atgcgaaagc agagattatc gtgtacccgg atgccgggca
    19861 tgcattcaac gctgattatc gcccgagcta tcatgccgaa tctgcgaaag atggctggca
    19921 gcgtatgctg gaatggttta agcagtatgg agagaagcgg agttagtttc ctcttagtct
    19981 atatttgaga tggtgagtaa acgagtgcgt tttgctgcca tctctgtccc ccaaaaaata
    20041 atcagtatga attgattgga gattacgact ccgctatttg accttaagaa agtcggcaag
    20101 tcatagtcat aaatgagtct cttcaatgga agacacctta gagatttacc atcaactccc
    20161 attcacaaac aaacattaat tattatcaac acattcacat taattataaa tcatctcaga
    20221 ccttcctgat aaaaagatat taaagtgaaa cattttgttt ttcacatttt agttcatcac
    20281 ttttaggcgt aaataaaggt aaaaaaaaca acaatactat ttctctttca caaaagaatt
    20341 aacaaaaggt ttttcaagga tgagtgatat tatttacctg accataaccg gagaacaaca
    20401 aggatgtatt tcctctcgtt gtggaacgag cgcatcaata ggtaatcgct ggcagattgg
    20461 ccatgatgat gaaatcttcg cgttctcgct ctctaatagt attacgaata caggcaaagg
    20521 gtcataattg catggcctta gtttctgtaa attgattgac aaaagctccc cgttacttat
    20581 taacgctatt aacaataacg agcagttgtt catggagttt gacttctatc ggatcaatag
    20641 gtttggtcga tgggagaaat attacaacat tcaactccgc ggcgcgctct tatcagcaat
    20701 caatcacctg tttactgaga acaatctgga tacagaagcc atcactgtca gttattaata
    20761 tatactctcc aggcatctga ttgctaatac tgaatttagt tattgggcat ttcccgataa
    20821 ctacaaccgt ctgtttatac cacggccaaa aactaaagct ggtaatggtt tcaaaacact
    20881 taatagtaaa gcggttggca gactgcttgc tgctgtcggc atatacaatg ggaatatcga
    20941 aggattcagg gaaacagcag acaagttggg gggagatgca cctgctggtt atgatcaatt
    21001 tatggataac aaagggttac tcatcgctgg tgcttcaata gttgctggag cagttctggg
    21061 gagaatgaga ctccctgatt tggaatcact tgagcatttc ggagccagag gagcagtatc
    21121 aggaagacca tttaatcccg agcttgccgg tggtccaata gaaaacctga cgacagatgg
    21181 cgtaacaatc aacagggaag gtatcgctat tgttgaaaaa catatagcca gatttggtca
    21241 tgatcctgta aatgaagtga tgatcaatag actgaaagac attgagaaag gaaaaatccc
    21301 cccagaacaa gtagatctta acttctatac tcatgagtgt cgggaatatc agcgttactg
    21361 taatcttggt tgggaaacag gacaacctga tggtgacgca ggctatgcac tctggaatca
    21421 tactggattt gcccctatat ttccagacat ctgttatcac ttaacccatt acaagcccgc
    21481 tgcc
//
LOCUS       DAZPJR010000060        21005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPJR010000060 DAZPJR010000000
VERSION     DAZPJR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.061"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..172)
                     /locus_tag="ACHVB7_002120"
     CDS             complement(<1..172)
                     /locus_tag="ACHVB7_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR1914105.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            344..1564
                     /gene="coaBC"
                     /locus_tag="ACHVB7_002121"
     CDS             344..1564
                     /gene="coaBC"
                     /locus_tag="ACHVB7_002121"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR1914106.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1542..2000
                     /gene="dut"
                     /locus_tag="ACHVB7_002122"
     CDS             1542..2000
                     /gene="dut"
                     /locus_tag="ACHVB7_002122"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR1914107.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2107..2703
                     /gene="slmA"
                     /locus_tag="ACHVB7_002123"
     CDS             2107..2703
                     /gene="slmA"
                     /locus_tag="ACHVB7_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR1914108.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="ACHVB7_002124"
     CDS             complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="ACHVB7_002124"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR1914109.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3447..4163)
                     /gene="rph"
                     /locus_tag="ACHVB7_002125"
     CDS             complement(3447..4163)
                     /gene="rph"
                     /locus_tag="ACHVB7_002125"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR1914110.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4290..5153
                     /locus_tag="ACHVB7_002126"
     CDS             4290..5153
                     /locus_tag="ACHVB7_002126"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR1914111.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5375..6199
                     /gene="dinD"
                     /locus_tag="ACHVB7_002127"
     CDS             5375..6199
                     /gene="dinD"
                     /locus_tag="ACHVB7_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR1914112.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6491..7108
                     /locus_tag="ACHVB7_002128"
     CDS             6491..7108
                     /locus_tag="ACHVB7_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR1914113.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="ACHVB7_002129"
     CDS             complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="ACHVB7_002129"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR1914114.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9045..9668
                     /gene="gmk"
                     /locus_tag="ACHVB7_002130"
     CDS             9045..9668
                     /gene="gmk"
                     /locus_tag="ACHVB7_002130"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR1914115.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9723..9998
                     /gene="rpoZ"
                     /locus_tag="ACHVB7_002131"
     CDS             9723..9998
                     /gene="rpoZ"
                     /locus_tag="ACHVB7_002131"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR1914116.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10017..12125
                     /gene="spoT"
                     /locus_tag="ACHVB7_002132"
     CDS             10017..12125
                     /gene="spoT"
                     /locus_tag="ACHVB7_002132"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR1914117.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12132..12821
                     /gene="trmH"
                     /locus_tag="ACHVB7_002133"
     CDS             12132..12821
                     /gene="trmH"
                     /locus_tag="ACHVB7_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR1914118.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12827..14908
                     /gene="recG"
                     /locus_tag="ACHVB7_002134"
     CDS             12827..14908
                     /gene="recG"
                     /locus_tag="ACHVB7_002134"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR1914119.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="ACHVB7_002135"
     CDS             complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="ACHVB7_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR1914120.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16562..17953
                     /gene="xanP"
                     /locus_tag="ACHVB7_002136"
     CDS             16562..17953
                     /gene="xanP"
                     /locus_tag="ACHVB7_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR1914121.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTTLKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18074..19783
                     /locus_tag="ACHVB7_002137"
     CDS             18074..19783
                     /locus_tag="ACHVB7_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1914122.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="ACHVB7_002138"
     CDS             complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="ACHVB7_002138"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1914123.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5084 a         5713 c         5386 g         4822 t
ORIGIN      
        1 agccataaag agagccgaaa ttctccagca tctcttttgc cagggttaat acatctttac
       61 cgcgcgttcc ggtacgcaga aatagcgcca gcagctcgac atccgttaag gcgctaatac
      121 caaacttcag cattttttcg cgcggcatca acagctgtgc attgtttttc accttcacct
      181 cctttgtggt gctcgcatcc tgtcatagcc gtttcgttaa atcgacggcc agttttcaat
      241 cctggaaagc gcctcgcaaa gtgaatctca ggtgaaggca cgacaacaaa agtattgtga
      301 taaaatcgcc aacttctggt gtcaccctac aggaaaaatc atcatgagcc tggccggtaa
      361 aaaaatcgtt ctcggcgtta gcggcggtat tgctgcctat aaaacccctg aactggtgcg
      421 tcgtttgcgc gatcgcgggg ccgacgtccg cgtagccatg accgaagcgg caaaagcctt
      481 tatcacccca cttagcttgc aggcggtttc tggttatccc gtttccgaca gtctgctgga
      541 cccggcagcc gaagccgcta tgggccatat tgagctgggt aaatgggctg atttagtgat
      601 tctcgcccct gccacggcag atttgattgc ccgtgttgct gccggaatgg cgaatgacct
      661 ggtatcgacg atttgtctgg ctacacctgc gcctgtagcc gtgctccccg ccatgaacca
      721 gcagatgtac cgtgccgctg ccacgcagca taatttagag gtgcttgctt cccgtggttt
      781 gctcatctgg gggccagaca gtggcagtca ggcttgtggt gatatcggtc ctgggcgaat
      841 gctcgatccg ttaaccattg tggatatggc ggtagcgcat ttttcgcccg tcaacgacct
      901 gaaacatctg aacattatga ttaccgccgg cccgacgcgt gaaccgctcg atccggtgcg
      961 ttatatctct aatcacagct ccggcaagat gggttttgct atcgccgccg ccgctgcccg
     1021 tcgtggcgcg aacgtcacgc tggtatcagg cccggtttcg ctaccgacgc caccgtttgt
     1081 taaacgtgtt gatgtgatga ccgcgctgga aatggaagtc gccgtgaatg cttctgtaca
     1141 gcagcaaaat atttttatcg gctgcgccgc cgtggcggat tatcgcgcag ctaccgtggc
     1201 cccagagaaa atcaaaaagc aggccacgca gggtgatgaa ttaacaataa aaatggttaa
     1261 aaaccccgat atcgtcgcag gcgttgccgc actaaaagac catcgaccct acgtcgttgg
     1321 atttgccgcc gaaacaaata atgtggaaga atacgcccgg caaaaacgta tccgtaaaaa
     1381 ccttgatctg atctgcgcga acgatgtttc ccagccaact caaggattta acagcgacaa
     1441 caacgcatta caccttttct ggcaggacgg agataaagtc ttaccgcttg agcgcaaaga
     1501 gctccttggc caattattac tcgacgagat cgtgacccgt tatgatgaaa aaaatcgacg
     1561 ttaagattct ggacccgcgc gttgggaagg aatttccgct cccgacttat gccacctctg
     1621 gctctgccgg acttgacctg cgtgcctgtc tcgacgacgc cgtagaactg gctccgggtg
     1681 actctacgct ggttccgacc gggctggcga ttcatattgc cgatccttca ctggcggcaa
     1741 tgatgctgcc gcgctccgga ttgggacata agcacggtat cgtgcttggt aacctggtag
     1801 gattgatcga ttctgactat cagggccagt tgatgatttc cgtgtggaac cgtggtcagg
     1861 acagcttcac cattcaacct ggcgaacgca tcgcccagat gatttttgtt ccggtagtac
     1921 aggctgaatt taatctggtg gaagatttcg acgccaccga ccgcggtgaa ggcggctttg
     1981 gtcactctgg tcgtcagtaa cacatacgca tccgaataac gtcataacat agccgcaaac
     2041 atttcgtttg cggtcatagc gtgggtgccg cctggcaagt gcttattttc aggggtattt
     2101 tgtaacatgg cagaaaaaca aactgcgaaa aggaaccgtc gcgaggaaat acttcagtct
     2161 ctggcgctga tgctggaatc cagcgatgga agccaacgta tcacgacggc aaaactggcc
     2221 gcctctgtcg gcgtttccga agcggcactg tatcgccact tccccagtaa gacccgcatg
     2281 ttcgatagtc tgattgagtt tatcgaagat agcctgatta ctcgcatcaa cctgattctg
     2341 aaagatgaga aagacaccac agcgcgcctg cgtctgattg tgttgctgct tctcggtttt
     2401 ggtgagcgta atcctggcct gacccgcatc ctcactggtc atgcgctaat gtttgaacag
     2461 gatcgcctgc aagggcgcat caaccagctg ttcgagcgta ttgaagcgca gctgcgccag
     2521 gtattgcgtg aaaagagaat gcgtgagggt gaaggttaca ccaccgatga aaccctgctg
     2581 gcaagccaga tcctggcctt ctgtgaaggt atgctgtcac gttttgtccg cagcgaattt
     2641 aaataccgcc cgacggatga ttttgacgcc cgctggccgc taattgccgc gcagttgcag
     2701 taatatgatg ccggatgact tttcatccgg cgagtttctt taaacgccaa actcttcgcg
     2761 ataggcctta accgccgcca gatgttccgc catttccggc ttctcttcca ggtaagcaat
     2821 caggtctttc agggtgatga tagagatcac tttgcagttg taatcacgct caacttcctg
     2881 aatcgccgaa atctcgctgc gcccccgttc ctgacgatcg agcgaaatca acacgccagc
     2941 aagcgtcgcg ccattggcct gaataatttc catcgactca cgaatcgccg ttccggcggt
     3001 gatcacatca tctaccagca ttacgcgtcc ttgtaacgcg ctaccaacca gattgccgcc
     3061 ttcaccgtgg ttttttgcct ctttgcggtt aaagcagtac ggcaggtcca ggtcatgatg
     3121 ctccgccagc gccacggcgg ttgtggtggc aatcgggatc cctttgtaag cagggccaaa
     3181 cagcagatcg aactcaatgc cggaatccac caatgcttca gcgtaaaaac ggcctaacag
     3241 tgccaggtcg cgcccggtat taaacagccc ggcgttgaag aaataggggc ttttacgccc
     3301 ggatttcagc gtaaactcgc caaactttaa cacctgcttg ctaagcgcaa attcaataaa
     3361 ctggcgctga tatggtttca tgccttcgct cctcatctta cttttctaca gacaaaaaaa
     3421 aggcgactca tcagtcgcct taaaaatcag tttgccagcg ccgccttctg cgtcgctaca
     3481 atggattcga ttccccctcg ggccagagcc aacaaggtga gtaattcttc gtgggtgaac
     3541 ggttcgcctt ctgccgtccc ctgcacttca atgatgcgcc cgtcttcggt catcactacg
     3601 ttcatgtcgg tctctgcggc agagtcttca acgtattcca gatcgcaaac cgcttcgccg
     3661 ttcacaatcc cgacagaaac cgcggctacc atccctttca tcggattggt tttcagctta
     3721 ccgttttcca ccagcttctg tagcgcatct gccagcgcca cgcaggcacc cgtaatcgaa
     3781 gcggtacgag tgccaccatc agcctgaagc acgtcgcagt ccagcgtaat ggtgaactca
     3841 cccagcgctt tcaaatctac tgccgcgcga agcgcacggg cgatcagacg ctggatttcc
     3901 attgtgcgtc caccctgctt acctttcgcc gcttcacgag cgttacgggt gtgggtagaa
     3961 cgcggcagca taccgtactc tgcggtgatc cagccctggc cctgaccttt caggaagcgc
     4021 ggcacgcctt cttcaataga ggcggtacac aacactttgg tatcgccaaa ttcgaccagc
     4081 accgagcctt ctgcatgttt tgtatagtta cgagtcaggg taacgggacg cacctgatta
     4141 ttgctacggc ctgctggacg catattgaaa tctccggctt gaaacaaatg tggctgcgca
     4201 ttatacggac ttccggaggt tattcctatc ctgacaaggc atcgatggct ataatccttc
     4261 cacctctcct tttataaaca ggaacgtcta tgatccgcag tatgaccgcc tacgcccggc
     4321 gtgaaatcaa gggtgaatgg gggagcgcaa cctgggaaat gcgctcggta aaccagcgtt
     4381 atctggaaac ttactttcgt ctgccggagc agttccgtag ccttgaacct gtcgttcgcg
     4441 agcgtattcg ttctcgcctg acgcgcggta aagtggaatg taccctgcgc tatgagccag
     4501 atgttagcgc gcaaggtgag ctgatcctca acgaaaaact ggctaaacag ctggtaactg
     4561 ccgcgaactg ggtaaaaatg cagagtgacg aaggggaaat caacccggtt gatattctgc
     4621 gctggccggg cgtaatggca gcccaggagc aggatctcga cgcgattgcc gctgaaattc
     4681 tcgcggcgtt ggatggtacc ctggacgact ttatcgtcgc acgtgaaacc gaaggtcagg
     4741 cgctgaaagc gttgatcgag cagcgtctgg aaggcgtcac cgccgaagtg gtcaaagtcc
     4801 gcgcccatat gccggaaatc ctgcaatggc agcgtgagcg tctggtcgcg aagctggaag
     4861 atgctcaagt gcaactggaa aacaaccgcc tggagcagga gttggttctg ctggcacaac
     4921 gaattgatgt tgccgaagaa ctggaccgcc tggaagcgca tgtcaaagag acctacaaca
     4981 ttctgaagaa aaaagaagcc gttggtcgtc gtctggactt tatgatgcaa gagttcaacc
     5041 gcgagtcgaa cactcttgcg tcgaaatcta tcaatgccga agtgacgaat tctgccatcg
     5101 agctgaaagt gttgattgag cagatgcgcg agcagattca gaatattgag taactcgtaa
     5161 cactaattct tacctttcag ccttcacaaa tgccgcccac tcaaacagag cggcattttt
     5221 cttccccgca acattcaatt ctgttttgcg tacctgctcc agattttgcg atgttttttt
     5281 gcgcagcgca gtgagaacgt gagatactca caactgtata taaatacagt tatagactta
     5341 ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc aaccttttga
     5401 agaaatacgg cattatggca ctgaagggca agaattttgg tcagcgagag aattggcacc
     5461 gttactggat tatcgggact ggcgcaattt tcaaaaggta ctggcccgcg cgactcaagc
     5521 gtgcgaagcc agcaatcaag ctgcatctga ccatttcgtt gaaaccacca aaatggttgt
     5581 attaggttca ggggctcaac gagaacttga agatgttcat ctctctcgct atgcctgtta
     5641 cctggtagta caaaacggcg accctgcgaa accggttatt gcggcagggc aaacttattt
     5701 tgctatccag acccgacggc aggagctggc tgacgatgaa gctttcaggc aacttcgtga
     5761 agacgaaaag cgtctttttc tgagaaatga gttgaaagaa cataacaaac aattagttga
     5821 ggcagcgcag caagctgggg tagcgacagc tatcgatttc gccatatttc agaatcatgg
     5881 ttaccggggg ctgtatggtg gattagatca gaaagccatc catcagcgga aggggctgaa
     5941 aaagagtcag aagatcctgg atcatatggg ttcaacagaa ctggcggcta atctctttcg
     6001 agctacccaa acagaagaaa aactcaagcg ggatggcgta aattcaaaac agcaagctaa
     6061 taccacacac tttgacgtgg gtagcaaggt gaggcaaacc attcaggaac ttggcggaac
     6121 catgcctgag gagttgccga ccccgcaggt cagcatcaat cagttggaaa acagtgtaaa
     6181 aattacagag aagaaatagc agaaactaaa aactcttttt cttaattgag atacccgatg
     6241 cgtaagccaa agtccaggtg cagtgaacac aatggctaaa cattgcacct ttcttttcca
     6301 cccctcaatt ttaacctatt ttttcttatg cattttctca gacaagaagt cagaagaatg
     6361 catctacact acagaaaata gcgatttcac ataactacaa gttatcaatt tcctctcacc
     6421 ttaaaaaatc tcaatcgtga caatacgcac aaatcgctac cctgccagac agatttttag
     6481 ggagagaacc atgctgttac acattttgta tttggttggc attactgccg aagccatgac
     6541 gggggcgctg gcggccggac gacggcgcat ggatacattt ggcgtaatta ttattgcgac
     6601 cgccaccgca attggcggag ggtcagtgcg cgatattctg ctgggccact atccgctcgg
     6661 ttgggtcaaa cacccggaat atgtgattat cgtcgctacc gccgcagtac tcaccactat
     6721 cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg gtgctcgatg cgctcggact
     6781 ggtggtcttt tctatcatcg gcgcacaggt tgcactggat atggggcacg gcccaattat
     6841 tgccgttgtc gcggcggtaa ccaccggcgt gtttggcggc gttttgcgcg atatgttctg
     6901 taaacgcatc ccactggtat tccagaaaga gttgtatgcc ggtgtctcat ttgcctccgc
     6961 cgtgctgtac atcgcgctgc aacactatgt ttctaaccat gatgtggtga ttatctccac
     7021 cctggtattc ggcttttttg cccgcttact ggcgctacgt cttaagctgg gattaccggt
     7081 cttttactac agccacgaag gtcactaagg ttcaaaacct gtgatctgct gggcagccag
     7141 ccaactgccc agcttcttga tttgcgcatt ttccttccat tcaataacct gtctggcgcg
     7201 cccagatcca gtccccggca gccgctgcca gaactgctcc gtgctaaata aaagttgcga
     7261 ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt aacggtattc ccattgccat
     7321 cacccagcga gtaaaaggct gcttacgagc cagattaaac tgatgccata actgcgcact
     7381 tttacttttc gcgatccccg gcgtgttctg taattgctct ggcgttaata aaagccagga
     7441 aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc cagccggcct caccaatgcc
     7501 atccagccca agaacctgtt ttgaccccag ccagactaag cgtgaaatga actgttcctg
     7561 acaaacatca gaagcaaagt agcaggtcaa cgagttaaag cggttttctg gtggtgtcgg
     7621 ttttgtacgt tctgcaccgc gccagaccac atcatcaatg cgaggaatac cctgaccggc
     7681 aaggctgacg aggatctgat caccaggcgc aatatcccac tcctgccagc gcctgacgga
     7741 accaatattc acccgctgga cttttttatc atccagcatg acaggtgcga gtgacgcaac
     7801 caccgatatt ttaccgctct tacccaccgc aaactgaatc gccttcactt cggcaacctg
     7861 agctacaggt tgatatttcc aggccaccaa ccattctgcc tggcctggta gccaatggcg
     7921 ggattcgggt tccttagccg ctcgcacaac cacgccatcg gtgacgaagg gtaatttcgc
     7981 tttccaccac tcattgcgta cgcgcgcaat ttcatcagca tttttaaccg cacgggtata
     8041 cctctgcgtt agagtaaaac ctgcggtagc cagctccttt aaacgatcgg tcattaactg
     8101 cggtccatcc ggccatgccc agacaaaaac agccagtgag tttagcgtgt cgctattccc
     8161 ctggcgcatc atcaggccag caacttttgc gcgggcattt attcccccca tttgttgttg
     8221 gatatgcccc tcgcgctgga gaaatatttc cccctgaagc gtactgttgg ctaaaggccc
     8281 gctaacggtt tgcggcacag cggaaattaa gctaactttc tgcgtccagt cctcgccttt
     8341 caggccatta ccgcgactga ttgctttgtt cagtttcccg tcccgataaa ccagggttac
     8401 cgcaacgcca tcaacttttg gctgcaccca aagatcgcta cgttctcgca tccacagact
     8461 taatgcgttt ttatccacca ttttgcgtac acccgtatgg gcaaccggat gcataaccgc
     8521 accatttaaa ggcggcatca tcacgtcgcg gggctcgctc ccaaagcagc gttgccactg
     8581 cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc acctcacttt tcccttcctt
     8641 ccagtagtca tcgtcccact gttttatttg ctgttgcagg cgggaaattt cttcctgtgc
     8701 tctggctggc gaccaggccg gacagaccgc ccacacagat gattgccagc acaagatact
     8761 tattaatatc gccatccata ctttcatcat cacctccgct gtagatagtc aggcagatat
     8821 acaacgtgat gaaagcaaag ccgagtggca aaaacggagt ctgcgaggac gcttcctgaa
     8881 aatcgtcttt attgcaatga atgacaggca aatgcggaag cagctacgca aaacgcaaca
     8941 actttgcgca aaaagtgtga gcaagggcta cgtcacatgg ccgcgccgtg tataataagc
     9001 tcgtatgtag gctttatttc gctaatcaca tacgaaagat actcatggct caaggcacgc
     9061 tttatattgt ttctgccccc agtggtgcgg gtaaatccag cctgattcag gctttattaa
     9121 aaacccaacc gttgtatgac acccaggttt ctgtttcaca caccacacgc caaccgcgtc
     9181 ctggtgaagt ccacggtgaa cattatttct ttgttaatca tgatgaattt aaagaaatga
     9241 ttagcagaga tgcgttcctc gaacacgcag aagtttttgg taattactat ggcacttcgc
     9301 gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt ttttctcgat atcgactggc
     9361 agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg gagtatcttt attttaccgc
     9421 cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg tcaggacagc gaagaggtca
     9481 ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca ttacgccgaa tatgattatc
     9541 tgattgtgaa tgatgacttc gataccgcgt tgaccgattt gaagaccatt attcgcgccg
     9601 aacgtctgcg catgagccgc caaaagcagc gtcatgacgc tttaatcagc aaattgttgg
     9661 cagactgaac ctgatttcag tatcatgccc agtcatttct tcacctgtgg agctttttaa
     9721 gtatggcacg cgtaactgtt caggacgctg tagagaaaat tggtaaccgt tttgacctgg
     9781 tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac
     9841 cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca
     9901 acaaccagat cctcgacgtt cgcgaacgcc aggaacagca agagcaggaa gccgctgaat
     9961 tacaagccgt taccgctatt gctgaaggtc gtcgttaatc acaaagcggg tcgcccttgt
    10021 atctgtttga aagcctgaat caactgattc aaacctacct gccggaagac caaatcaagc
    10081 gtctgcggca ggcgtatctc gttgcacgtg atgctcacga ggggcaaaca cgttcaagcg
    10141 gtgaacccta catcacgcac ccggtagcgg ttgcctgcat tctggccgag atgaaactcg
    10201 actatgaaac gctgatggcg gcgctgctgc atgacgtgat tgaagataca cccgccacct
    10261 accaggatat ggaacagctt tttggtaaaa gcgtcgccga gctggtagag ggggtatcga
    10321 aacttgataa actcaagttc cgcgataaga aagaggcgca ggccgaaaac tttcgcaaga
    10381 tgattatggc gatggtgcag gatatccgcg tcatcctcat caaacttgcc gaccgtaccc
    10441 acaacatgcg cacgctgggc tcacttcgcc cggacaaacg tcgccgcatc gcccgtgaaa
    10501 ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat ccaccacatt aaaaccgaac
    10561 tcgaagagct gggttttgag gcgctgtatc ccaaccgtta tcgcgtaatc aaagaagtgg
    10621 tgaaagccgc gcgcggcaac cgtaaagaga tgatccagaa gattctttct gaaatcgaag
    10681 ggcgtttgca ggaagcggga ataccgtgcc gcgtcagtgg tcgcgaaaag catctttatt
    10741 cgatttactg caaaatggtg ctcaaagagc agcgttttca ctcaatcatg gacatctacg
    10801 ctttccgcgt gatcgtcaat gattccgaca cctgttatcg cgtgctcggg caaatgcata
    10861 gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat cgccattcca aaagcgaacg
    10921 gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg tgtgccggtt gaggtccaga
    10981 tccgtactga agatatggat cagatggcgg agatgggtgt tgccgcgcac tgggcttata
    11041 aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc ccagcgctgg atgcaaagcc
    11101 tgctggagct gcaacagagc gccggtagtt cgtttgaatt tatcgagagc gttaaatccg
    11161 atctcttccc ggatgagatt tacgttttca caccggaagg gcgaattgtc gagctgcctg
    11221 ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac cgatatcggt catgcctgcg
    11281 tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca gccgcttacc agcggtcaaa
    11341 ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc cgcgtggctg aactttgtcg
    11401 ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa aaacctcaag cgtgatgatt
    11461 ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg tggtagccgt aagctgaatg
    11521 aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat gaagctggca acgcttgacg
    11581 atctgctggc agaaatcgga cttggtaacg caatgagcgt ggtggtcgcg aaaaatctgc
    11641 aacatgggga cgcctccatt ccaccggcaa cccaaagcca cggacatctg cccattaaag
    11701 gtgccgatgg cgtgctgatc acctttgcga aatgctgccg ccctattcct ggcgacccga
    11761 ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca ccatgaatcc tgccgtaata
    11821 tccgtggcta ccagaaagag ccagagaagt ttatggctgt ggaatgggat aaagagacgg
    11881 cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa tcatcagggc gcgctggcaa
    11941 acctgacggc ggcaattaac accacgactt cgaatattca aagtttgaat acggaagaga
    12001 aagatggtcg cgtctacagc gcctttattc gtctgaccgc ccgtgaccgt gtgcatctgg
    12061 cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat taaagtcacc cgaaaccgaa
    12121 attaatgttt tatgaaccca acacgttatg cacgcatctg cgaaatgctc gccaggcggc
    12181 agcctgatct gaccgtctgc atggagcagg tccacaaacc tcataacgtt tctgcgatta
    12241 ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc cgtctggcct ggtagccgca
    12301 tgcgcaccat ggcttcggca gcggcgggta gtaacagctg ggtacaggtg aaaacacacc
    12361 gcaccattgg cgatgccgtc gctcatctga aaggccaggg tatgcagatt ctggcaaccc
    12421 atctttctga taacgctgtc gatttccgcg aaattgatta cactcgcccg acctgcattt
    12481 tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt ggccctggcg gatcaggaca
    12541 tcatcattcc gatgatcggc atggtgcagt cgctgaatgt ttccgttgcc tcagccctca
    12601 ttctttacga agcccagcgt cagcggcaaa atgcaggcat gtacctgcgt gaaaacagca
    12661 tgttgccgga agcagagcaa caacgcctgt tgtttgaagg cggctatccg gtgctggcga
    12721 aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa tcagcaaggc gagatcgaag
    12781 ctgatgccga ctggtgggct actatgcagg ctgcagggta agtgccatga aaggtcgcct
    12841 gttagatgct gtcccactca gttccctaac gggcgttggc gcagcactta gtaacaaact
    12901 ggcgaaaatc aacctgcata ccgtgcagga tctactctta caccttcccc tgcgctacga
    12961 agatcgcacc catctctacc ccatcggaga actactgccg ggcgtttatg ccacggtgga
    13021 aggcgaagtg ctgaactgca atatctcctt cggcggtcgg cggatgatga cctgccagat
    13081 cagcgacggt tccggcatcc tcaccatgcg ctttttcaat ttcagcgcgg caatgaaaaa
    13141 tagcctggcg acgggccgcc gtgtactggc ttatggcgaa gcaaagcgcg gtaaatatgg
    13201 tgcggagatg atccacccgg aataccgcgt gcagggcgat ctcagcacgc cagaattaca
    13261 ggaaacgctc acgccggttt atccaacaac ggaaggcgta aagcaggcca cgctgcgtaa
    13321 attaaccgac caggcgctgg atctgctcga cacctgcgcc attgaagaac tcctgccgcc
    13381 ggaactgtca caaggaatga tgacgctacc ggaagcgttg cgcactttgc accgcccgcc
    13441 accgacgcta cagcttagcg atctggaaac cgggcagcat ccggcgcaac gtcgtctgat
    13501 tctggaagaa ctgctggcgc acaacctcag catgttagcc ttacgtgccg gagcacagcg
    13561 ttttcatgcc cagccgctga gcgccaatga tgcgctgaaa aataaactcc tcgcagcctt
    13621 accgttcaag ccaacgggcg cacaggcacg cgtagtggcg gagatcgagc gcgatatggc
    13681 gctggatgtg ccgatgatgc gcctggtaca gggcgatgta ggttccggta aaacgctggt
    13741 cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa caggtggcat tgatggcacc
    13801 aaccgaatta ctcgccgaac agcacgccaa taacttccgc aactggtttg aaccgctcgg
    13861 tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa gcgcgactgt ctcagcagga
    13921 agccatcgcc agcggtcagg tgcagatgat tgttggtacg cacgccatct tccaggaaca
    13981 ggtgcagttt aacggtctgg cgctggtgat tatcgacgaa caacatcgct ttggcgtgca
    14041 tcagcgtctg gcgttatggg agaaaggcca gcagcaaggc ttccatccgc atcagttgat
    14101 catgaccgcc acgccgatcc cccgcacgct ggcaatgacg gcgtatgccg atctcgatac
    14161 ctcggtgata gatgaactgc cgccaggccg cacgccagtg actacggtcg ctattcctga
    14221 tactcgccgt accgacatca ttgaccgcgt gcgccacgcc tgcatgactg aaggtcgtca
    14281 ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta ctggaagcgc aggcggcgga
    14341 agctacctgg gaagagttga aactggcgct accagaattg aatgttggcc tggtacacgg
    14401 gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg tttaaacaag gtgagctaca
    14461 tctgctggtt gccactaccg ttattgaagt cggtgttgat gtgcctaacg ccagcctgat
    14521 gattatcgaa aaccctgagc gtctgggtct ggcgcagtta caccagctgc gcgggcgcgt
    14581 aggtcgtggc gcggtggctt ctcactgcgt gctactctac aaaacgccgc tttctaaaac
    14641 ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac ggttttgtga ttgcgcaaaa
    14701 agatctggag attcgcggcc ctggcgaatt gttaggcacg cgtcagacgg gtaatgctga
    14761 atttaaagtg gcggatttac tgcgcgatca ggcgatgatc ccggaagttc agcgcctggc
    14821 acgccatatt cacgaacgtt acccacaaca ggcaaaagcc ctgatagaac gctggatgcc
    14881 ggagacggaa cgttactcga atgcgtaaaa ggcggcagtg agaagaccgc catttcaggt
    14941 taccctacct tcctgccgga tgcgattcat caccctacaa attcaataaa ttatgaatca
    15001 atacgcaggc ttgataagcg aagcgtatca ggcatttgtc tttgttgcgg cggcacttct
    15061 accgccgctt catcggttaa ccggcaaaaa tcggcaacat caaatacaac ttaattacca
    15121 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    15181 gcgacgggcc aaagcgttca gtaatcgcct gcatgttggc gattgccgtt ggcgttgcac
    15241 cgaggccaaa accacagtga cctgcagcca gcactgccgc atcgtagttt ttgcccatca
    15301 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    15361 ccagcatcgg cagcgccagc gaagccagct cccacagttt cagccccatc aacgccatcg
    15421 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    15481 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    15541 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    15601 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    15661 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    15721 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    15781 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    15841 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    15901 acagccccat cagcggatca agccctaaca ggctagccat accaatgcca atggcatttt
    15961 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    16021 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    16081 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    16141 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    16201 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    16261 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    16321 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    16381 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    16441 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    16501 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    16561 gatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    16621 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    16681 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    16741 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    16801 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    16861 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtacca cgctgaaaac
    16921 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    16981 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    17041 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    17101 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    17161 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    17221 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    17281 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    17341 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    17401 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    17461 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    17521 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    17581 gatccagttg accggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    17641 cgtgctgggt ctgttcccgg cagtgagcgg ttttgtacaa cacattccag aaccggttct
    17701 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    17761 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    17821 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    17881 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    17941 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctcttta
    18001 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    18061 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    18121 agtggcgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    18181 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg ttcaccgttt
    18241 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gccagcccgc
    18301 gaccctggtg gccaaaagtg tcgacattgc gctaagtagt cggcaactga ccgaaccacg
    18361 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    18421 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    18481 cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    18541 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    18601 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    18661 gactaacctg ggtgccgaca tcgcgcgcgg gacattaacc ggaaacgcgc agcgtaacgc
    18721 cgacggcagc tggcaagtgg aaaacctgcg catggcggat atccgtctac aaagcgaaaa
    18781 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgcct
    18841 ggaagtgatc gatgctcgtt tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    18901 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caagaaggca aactgtcgat
    18961 gaacgctagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    19021 attttctccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    19081 cagaacgtca gggaactggc tgcgtgacgg gaaaacgttg atccttgatg atgcggcaat
    19141 tgccggactg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    19201 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    19261 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    19321 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    19381 cgcgacgttt aatcgtgttg atgtccgtcg tccgtcgctg gcgctaaccg ccaacagcag
    19441 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    19501 cagtgtttca caaacgccac aacgtcagac acatatcagc ctgaatggac gcggtgtgcc
    19561 ggtgaatatt ttgcaacagt ggggatggcc tgaattaccg ttgactggcg acggcaatat
    19621 tcagcttacc gccagtggcg atattcaggc caatgtcccg ttgaaaccta cggttagcgg
    19681 gcaattgcat gccgtgaacg ccgcaaagca gcaagtgact caaaccatga atgcgggcgt
    19741 cgtttccagc ggtgaagtta catcggcaga gctggtgcag taaacatcaa tctcgccggg
    19801 tacgactcag acgtacccgg cattcttcaa tagattacaa cgtaattgtc agcgcattcc
    19861 cttgaggcgt aaccaccagc ccctgctcac tttcagcctg cgaaccgcct tgcagaccat
    19921 ttactttcac aatattgcgc aagcacagtg tccagttcct cgcctcgccc gcaccagtca
    19981 cagtaatcgt gttaccagta cgtgttgctt ttagagtaaa gatcaccgaa ccgtcagcag
    20041 cgggcacttc acatacagct tcatgcccgt cttgcagatt aaagagatgg aatgccgtgc
    20101 cttcgtgcca cgcgtaatcg ggacgttgat cgttgttgcc cagcgccaat agggtgttat
    20161 cacgcacata aacgggcaga ctcaggaagc cgtgctgctg tttatgccag cgactaccgt
    20221 cgagctcatt gttgtgccac aggtgtgtcc agcgaccttc cggcaagtag aactgcacct
    20281 cgcccgcctc agtgaacacc ggagcaacca tcacgttgtc gcctagcatg tattgacggt
    20341 caaggtaatc acaagccgga tcgtccggga actccatcat catggcccgc atcatcggcg
    20401 taccccgcgc tttcgcacgc gcagcttcac gatacagata cggcatcatg cggcatttca
    20461 gttgcgtgaa gaagcgcacc acatcacagg actcatcatc gtaggcccac ggcacacgat
    20521 aagatttgct gccatgtaaa cgactatggc tggagagcaa accaaacgcg caccagcgtt
    20581 tgtaaacgtg tgccggcgcg gtattttcaa agccgccgat atcgtggctc cagaagccaa
    20641 aacctgaaag gccaatagac aaaccaccgc gcaggctttc cgccattgat tcgtagttag
    20701 cgtaacaatc gccaccccag tgtaccggga atttctgcgc accgacggag gccgagcggg
    20761 caaacaagac agcttcttcc tcaccaacgg tgtccttgag cacgttccac accagttcgt
    20821 tgtagatgta cgcataatgg ttatgcattt tctgcggatc ggaaccgtca aaccactgaa
    20881 catcagttgg gatacgttcg ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca
    20941 gacctttcag tttgtcggcg taccatttgc aggcatccgg attggtaaag tcataaatcg
    21001 ccaga
//
LOCUS       DAZPJR010000061        20711 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPJR010000061 DAZPJR010000000
VERSION     DAZPJR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.062"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHVB7_002139"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHVB7_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR1914124.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHVB7_002140"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHVB7_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR1914125.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHVB7_002141"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHVB7_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR1914126.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHVB7_002142"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHVB7_002142"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR1914127.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHVB7_002143"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHVB7_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR1914128.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHVB7_002144"
     CDS             complement(8214..9107)
                     /locus_tag="ACHVB7_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR1914129.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHVB7_002145"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHVB7_002145"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR1914130.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHVB7_002146"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHVB7_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR1914131.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHVB7_002147"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHVB7_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR1914132.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHVB7_002148"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHVB7_002148"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR1914133.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHVB7_002149"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHVB7_002149"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR1914134.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="ACHVB7_002150"
     CDS             complement(14275..16539)
                     /locus_tag="ACHVB7_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFR1914135.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHVB7_002151"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHVB7_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR1914136.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHVB7_002152"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHVB7_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR1914137.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHVB7_002153"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHVB7_002153"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR1914138.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHVB7_002154"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHVB7_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR1914139.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5732 c         5044 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacacc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAZPJR010000062        20572 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPJR010000062 DAZPJR010000000
VERSION     DAZPJR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.063"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1132
                     /gene="ypdA"
                     /locus_tag="ACHVB7_002155"
     CDS             <1..1132
                     /gene="ypdA"
                     /locus_tag="ACHVB7_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR1914140.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1147..1881
                     /gene="ypdB"
                     /locus_tag="ACHVB7_002156"
     CDS             1147..1881
                     /gene="ypdB"
                     /locus_tag="ACHVB7_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR1914141.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1894..2751
                     /locus_tag="ACHVB7_002157"
     CDS             1894..2751
                     /locus_tag="ACHVB7_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1914142.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="ACHVB7_002158"
                     /pseudo
     CDS             complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="ACHVB7_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="ACHVB7_002159"
     CDS             complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="ACHVB7_002159"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1914143.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="ACHVB7_002160"
                     /pseudo
     CDS             complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="ACHVB7_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7411..8658)
                     /locus_tag="ACHVB7_002161"
     CDS             complement(7411..8658)
                     /locus_tag="ACHVB7_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR1914144.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8680..9006)
                     /locus_tag="ACHVB7_002162"
     CDS             complement(8680..9006)
                     /locus_tag="ACHVB7_002162"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR1914145.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9226..10191)
                     /gene="glk"
                     /locus_tag="ACHVB7_002163"
     CDS             complement(9226..10191)
                     /gene="glk"
                     /locus_tag="ACHVB7_002163"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR1914146.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10395..11651
                     /locus_tag="ACHVB7_002164"
     CDS             10395..11651
                     /locus_tag="ACHVB7_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR1914147.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11766..12092
                     /gene="ypeC"
                     /locus_tag="ACHVB7_002165"
     CDS             11766..12092
                     /gene="ypeC"
                     /locus_tag="ACHVB7_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR1914148.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12139..13377)
                     /locus_tag="ACHVB7_002166"
     CDS             complement(12139..13377)
                     /locus_tag="ACHVB7_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR1914149.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13713..14915
                     /gene="nupC"
                     /locus_tag="ACHVB7_002167"
     CDS             13713..14915
                     /gene="nupC"
                     /locus_tag="ACHVB7_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR1914150.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(14965..17153)
                     /locus_tag="ACHVB7_002168"
                     /pseudo
     CDS             complement(14965..17153)
                     /locus_tag="ACHVB7_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(17362..17437)
                     /locus_tag="ACHVB7_002169"
     tRNA            complement(17362..17437)
                     /locus_tag="ACHVB7_002169"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17402..17404),aa:Ala,seq:ggc)
     gene            complement(17477..17552)
                     /locus_tag="ACHVB7_002170"
     tRNA            complement(17477..17552)
                     /locus_tag="ACHVB7_002170"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17517..17519),aa:Ala,seq:ggc)
     gene            17773..18132
                     /locus_tag="ACHVB7_002171"
     CDS             17773..18132
                     /locus_tag="ACHVB7_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1914151.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18134..18526
                     /locus_tag="ACHVB7_002172"
     CDS             18134..18526
                     /locus_tag="ACHVB7_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1914152.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18578..19993)
                     /gene="gltX"
                     /locus_tag="ACHVB7_002173"
     CDS             complement(18578..19993)
                     /gene="gltX"
                     /locus_tag="ACHVB7_002173"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR1914153.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20252..20327
                     /locus_tag="ACHVB7_002174"
     tRNA            20252..20327
                     /locus_tag="ACHVB7_002174"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20285..20287,aa:Val,seq:tac)
     gene            20372..20447
                     /locus_tag="ACHVB7_002175"
     tRNA            20372..20447
                     /locus_tag="ACHVB7_002175"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20405..20407,aa:Val,seq:tac)
     gene            20494..20569
                     /locus_tag="ACHVB7_002176"
     tRNA            20494..20569
                     /locus_tag="ACHVB7_002176"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20527..20529,aa:Val,seq:tac)
BASE COUNT         5111 a         5486 c         5115 g         4860 t
ORIGIN      
        1 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcaga ccaagctggc
       61 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
      121 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
      181 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
      241 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
      301 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
      361 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
      421 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
      481 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
      541 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
      601 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
      661 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
      721 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
      781 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
      841 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
      901 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
      961 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
     1021 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
     1081 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     1141 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     1201 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     1261 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     1321 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     1381 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     1441 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     1501 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     1561 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     1621 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     1681 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     1741 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     1801 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     1861 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     1921 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     1981 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     2041 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     2101 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     2161 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     2221 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     2281 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     2341 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
     2401 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
     2461 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     2521 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     2581 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     2641 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     2701 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     2761 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     2821 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     2881 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
     2941 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     3001 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     3061 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     3121 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     3181 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     3241 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     3301 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     3361 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     3421 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
     3481 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
     3541 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     3601 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
     3661 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
     3721 cataagatct gatcgaggct gttaaccatc gggatcatca actgggcgtt gccgaaactg
     3781 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
     3841 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     3901 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
     3961 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     4021 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
     4081 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     4141 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
     4201 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     4261 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
     4321 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
     4381 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
     4441 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     4501 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
     4561 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
     4621 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
     4681 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
     4741 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     4801 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     4861 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     4921 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     4981 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
     5041 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     5101 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
     5161 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     5221 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     5281 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     5341 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     5401 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
     5461 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
     5521 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
     5581 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
     5641 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
     5701 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
     5761 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
     5821 gctttcccca tcacccgctg gtgagggaga acctgaaaag ctgtatcaaa ggtgacgcga
     5881 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
     5941 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
     6001 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
     6061 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
     6121 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
     6181 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
     6241 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
     6301 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
     6361 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
     6421 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
     6481 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
     6541 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
     6601 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
     6661 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
     6721 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
     6781 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
     6841 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
     6901 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
     6961 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
     7021 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
     7081 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
     7141 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
     7201 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
     7261 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
     7321 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
     7381 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
     7441 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
     7501 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
     7561 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
     7621 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
     7681 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
     7741 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
     7801 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaagctgaa
     7861 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
     7921 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
     7981 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
     8041 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
     8101 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
     8161 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
     8221 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
     8281 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
     8341 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
     8401 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
     8461 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
     8521 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
     8581 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
     8641 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
     8701 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
     8761 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
     8821 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
     8881 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
     8941 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
     9001 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
     9061 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
     9121 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
     9181 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
     9241 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
     9301 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     9361 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
     9421 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
     9481 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9541 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
     9601 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
     9661 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     9721 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     9781 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
     9841 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     9901 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     9961 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10021 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10081 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10141 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10201 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10261 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    10321 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10381 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10441 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10501 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    10561 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    10621 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    10681 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    10741 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    10801 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    10861 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    10921 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    10981 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11041 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11101 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11161 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11221 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11281 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11341 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11401 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11461 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11521 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    11581 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    11641 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    11701 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    11761 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    11821 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    11881 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    11941 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12001 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12061 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12121 gatgcgacgc tcattcaact acaatcccag cgccgtcccc accaacaacc agatattcag
    12181 cgccacgacc agcactacaa tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag
    12241 atcgcccatc aacttgctgt cactggtgaa aatcagcagt ggaaccagcg ccagggcgat
    12301 accaaaactt aacagcacct gactcataac cagaatccgt gtcggatcta atcccatcag
    12361 aatgacaata aatgacggca acatggtgac tgtacgacgc acccacagcg ggatatggaa
    12421 gcgaatgaat ccctgcatca ccacctgccc cgccagtgtc cccaccaccg ttgaggacag
    12481 tccggcagca accagactta acccaaagac cgttgccgca gcatggctta acagcggttg
    12541 cagcgtcaga taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc
    12601 cgccgcagct gtagccatca tcgccagatt gacaaaaccg gcaatcgtca tggcgatagc
    12661 cacatcccat ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt
    12721 gagcgaagag tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc
    12781 caggaagacc gcctccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc
    12841 caggttaggc tgggagaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa
    12901 cccgccaatc actttctcca gtggtttttg cccgcgacgt tgcagcatta aaatcaggaa
    12961 agtcgcgatc cccgtcagca ccgcgccctg caacaacgag acaccaagaa tgagtttaaa
    13021 accgatcgcc gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg
    13081 aacccaatag aaccacacta cgggacgcgg atagtgatca cgaatctgct ccgccagatt
    13141 ttttccggtg gcaatcccta gtttggcaga gaggatctga atcagcatcg ccatcaggtt
    13201 ggcccaaacg acaacccaca gtagctgata gccgaagctg gcacccgcct gaatattggt
    13261 cgcaaagtta ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc
    13321 gagcctcatc ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt
    13381 gtgcctctaa aacatagcct ttgctatgtt tcatgctatg ataaacgaga atgattatca
    13441 aattcattta aatggattgt ggtgatttct ctgatagacc aggattatga ctacgaaaag
    13501 attgatgact tgaatgttat ggttatgttt aatgttagca catttacata actttcagct
    13561 tccatacaca acatagcaga aatgtatgac agatcactat ttttgaagcc tgtcacagga
    13621 cgtcattata gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg
    13681 gaaatttaac ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg
    13741 cacttgccgt tgttgcgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta
    13801 tccgttatgt tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact
    13861 ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctgctcagat
    13921 ttgccaacga agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct
    13981 tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc
    14041 acattcgcgt gttgccggtg attatccgcg caattggttt cctgctctcc aaagtcaacg
    14101 gtatgggcaa actggaatcc tttaacgccg tcagctccct gattctgggt cagtctgaaa
    14161 actttattgc ctataaagat atcctcggca aaatctcccg taatcgtatg tacaccatgg
    14221 ctgccacggc aatgtccacc gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg
    14281 aaccgaaata cgtcgttgct gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt
    14341 cgctgatcaa tccttaccgt gttgatgcca gtgaagaaaa catccagatg tccaacctgc
    14401 acgaaggtca gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg
    14461 ccattatcgt tgccgcgatg ctgatcggct ttatcgccct gatcgccgcg ctgaacgcac
    14521 tgtttgccac cgttactggc tggtttggct acagcatctc cttccagggc atcctgggct
    14581 acatcttcta tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg
    14641 gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga
    14701 aaattgcttc cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc ctggtttcct
    14761 tcgctaactt ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc
    14821 aaggtaacgt ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtgagtg
    14881 tgctgtctgc gtcaatcgca gcactggtgc tgtaaaacca tacataaaaa agccggggat
    14941 cattccccgg cttttttacg cctgttaatc agctaatggc tgcgggcgac caatcaaata
    15001 cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt gcgtctcgac
    15061 aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat
    15121 catcgcatcc agcgtattcg tgacaatatc tttcacaaag acgccatcaa ttttgatgat
    15181 atcagcctgc aaacgcttta accgttcgta gttggcatat ccggtgccaa agtcatcaat
    15241 cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt
    15301 agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga tgtgataacg
    15361 cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa
    15421 attgactgaa aagcgcggtc cttttttgtc gcaagggtgt gttgccagcc acttcaacaa
    15481 ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag
    15541 aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga tctcatcata
    15601 accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa
    15661 cgccgtgcga atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc
    15721 ctctctgcta tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct
    15781 taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc
    15841 accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt
    15901 aaccatatgc tggagtcgcc cttccgtttc cggcccgctc agcactaaca gcagttcact
    15961 acccggcaat tgatataact tttcgttttc ctgcatcaac ggcagcagcg tacggtaaat
    16021 tgatcggata cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt
    16081 atcaatgcgc aggcagcaaa actcttgccc gcctcttgct ccggcgcttg ttccaacgca
    16141 cggaagttgg gcaggagcgt taacggatcg gtcagcgcct gcaaatgcca gcggcgatta
    16201 agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct aaaggaaatc
    16261 agcaccgcaa gaataaatgc cagcgaatat tcggtttcca ccccttgcaa aaagttctgg
    16321 ttgtaattca gaaggcaaag cgttgaaaca gccctggtga gatttaaaaa cggatagcga
    16381 agcttaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaggta accggcaata
    16441 aagtcgtttt cataaggtgt gcacaacaga agtagaagca cgcttaacgc tgccagccag
    16501 cttaaggtaa atgcgcgttt ctctttgccc aacgacggcg cgatatccct gcgccacaat
    16561 atctgcgcaa agtggggact tacaatcatg cgagtgagat agtagaaaag catgttgtaa
    16621 ataagcacag cggtgaaaag gcttagcaaa tcaacgaccg tgaaaatggc atccgcatcg
    16681 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
    16741 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
    16801 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
    16861 aacacgacaa acgtctggct accgagcatg accgcagtct gtaaaaaaga gagatgaaag
    16921 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
    16981 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
    17041 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
    17101 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
    17161 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
    17221 caaacagggg gccagcctgt tgccgatttt tcatccaatg atgctttcac caggcggcac
    17281 taacccggac gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct
    17341 ttgcagcgag ggttggaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
    17401 tgccatgcaa gcgctctccc agctgagcta tagccccacg atgcgtttac gtaccaaatt
    17461 tgctgggtgc aaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca
    17521 tgcaagcgct ctcccagctg agctatagcc ccgtcacgta aagcttgtcg agttgacggg
    17581 cggcatcata tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat
    17641 cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag tagttattca
    17701 tgtcacggtt tcctgtaaag tggtgttata aaatgaacta ctaatagacc cacatacatt
    17761 cagggaattg ttatgttcaa ggagaggatg acaccagatg aacttgccag actgaccggc
    17821 tatagccgcc agaccattaa taaatgggta cgcaaggagg gctggacaac gtcaccaaaa
    17881 cctggcgtcc agggtggcaa agcccgactg gttcacgtca atgaacaggt tcgtgaatat
    17941 attcgcaatg ctgaacgtcc agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg
    18001 ccgcttgaag tattgctggt gacactggcg aaagagatga cgccagttga acaaaaacag
    18061 tttacatcct tgcttctgcg ggaagggatt atcggattgt tacaacgctt agggattcgc
    18121 gatagcaaat aatatgaaaa gattacgcaa taaaatgacc accgaagaac tggctgaatg
    18181 cctcggtgtg gctaaacaaa ctgttaaccg ttggatcaga gaaaaaggct ggaaaacgga
    18241 aaaatttccc ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg
    18301 cgagtttatt cagaacacgc cagccttcca taacacacca atgttgatgg aagctgaaga
    18361 gagaattgct gaatacgctc ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc
    18421 gatagataat atgacagaca ttgagcaggg aaaagtcgcg caatttttgt cacgtgaagg
    18481 aattcgcaat ttcctcgccc gtctcgatat tgacgaatca gcataaataa aaaacggcag
    18541 gatattatct cctgccgttt atctttttac acgctaatta ctgctgattt tcgcgttcag
    18601 caataaaatc cagcgctttg ttgatacgct cgatactgcg ggtcttacca attgcgtgaa
    18661 cggtaacatc cagtgctgga gactgccccg ccccggttac ggcgacacgc agcggcatac
    18721 caactttacc catacccact tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt
    18781 tttcagcggt ccagtcagta atcgcggcca gtttgtcacg aaccacttcc agcggctgac
    18841 gcgctaccgg acgcagatgt tttttcgccg catcggcatc gaactcagca aaatcttcgt
    18901 agaaataacg gcagctctgt gccatctctt tcagcgtctt gcagcgttcg cccagcagtt
    18961 tcaccagatc agccagctgc gggccgttac gggtatcgat attttcctgc tcaatgtgcc
    19021 actgtaagtg agtagcaaca tactccggcg gcagcgcgtt aatgtagtga tggttcagcc
    19081 acagcagctt gtcggtgttg aacgcactgg cagatttgct gacggcattc aaagtgaagt
    19141 atttgatcat ctcttcacga gtgaagattt cctgatcgcc gtgggaccag cccagacgca
    19201 ccagatagtt cagcagtgct tctggcaaat aaccgtcatc acgatactgc attacgctga
    19261 ctgccccgtg acgtttggac agttttttac cgtcatcgcc attgatcata gaaacgtgcg
    19321 cgtaaaccgg caccggcgct ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt
    19381 tgatatggtc ttcgccacgg ataacgtggg tgatttccat atcccagtca tcgacaacca
    19441 cacagaagtt ataggttggg gaaccatcgg tacggcggat aataagatcg tccagttcct
    19501 ggttgctgaa ctcgatcgga ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg
    19561 ggttagcaaa acgtacaaca cacggttcat catcagcatg atgctcatgg ctgtggcggc
    19621 agcgaccgtc ataacgcggc ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca
    19681 ggcgctcttt agagcaatag catttataag cagtgccctc ttccagcatc tgatcgatca
    19741 ccgcgttgta gcgatcaaaa cgtttggtct ggtagtacgg accttcatcc cactccaggc
    19801 tcagccagtt catgccatcc ataatggctt cgatagcttc cggcgtggaa cgctcaagat
    19861 cggtgtcttc aatacgcagc acgaactcac cgccgtggtt acgtgcaaaa agccaggagt
    19921 aaagagcagt acgcgcgccg ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag
    19981 ttttgatttt catgaaatgg ccttacgttt agaaagatgc cgacaaccgg caaatcctgg
    20041 aaaaattgag tgggcgatat tctatcactc ccgcctgatt cctcaatgta gttcgggttt
    20101 ctaccttacg cattattgtt ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca
    20161 tcttgcttaa atttacgacg aacgaacaat ttctttagaa aatgcgttga ctcattttga
    20221 actctcccta taatgcggct ccacacagcg ggggtgatta gctcagctgg gagagcacct
    20281 cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccaact actttatgta
    20341 gtctccgccg tgtagcaaga aattgagaag tgggtgatta gctcagctgg gagagcacct
    20401 cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccaatt tctcgccagc
    20461 taaatttctt gtaaaaatgt gaagtaccga agtgggtgat tagctcagct gggagagcac
    20521 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac tt
//
LOCUS       DAZPJR010000063        20542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPJR010000063 DAZPJR010000000
VERSION     DAZPJR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.064"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="ACHVB7_002177"
     CDS             complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="ACHVB7_002177"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR1914154.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="ACHVB7_002178"
     CDS             complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="ACHVB7_002178"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR1914155.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="ACHVB7_002179"
     CDS             complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="ACHVB7_002179"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1914156.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4042..4800
                     /gene="loiP"
                     /locus_tag="ACHVB7_002180"
                     /gene_synonym="yggG"
     CDS             4042..4800
                     /gene="loiP"
                     /locus_tag="ACHVB7_002180"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR1914157.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4943..5863)
                     /gene="speB"
                     /locus_tag="ACHVB7_002181"
     CDS             complement(4943..5863)
                     /gene="speB"
                     /locus_tag="ACHVB7_002181"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR1914158.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6001..7977)
                     /gene="speA"
                     /locus_tag="ACHVB7_002182"
     CDS             complement(6001..7977)
                     /gene="speA"
                     /locus_tag="ACHVB7_002182"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR1914159.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="ACHVB7_002183"
     CDS             complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="ACHVB7_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR1914160.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8211..8354)
                     /gene="yqgG"
                     /locus_tag="ACHVB7_002184"
     CDS             complement(8211..8354)
                     /gene="yqgG"
                     /locus_tag="ACHVB7_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR1914161.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8465..8716)
                     /gene="yqgD"
                     /locus_tag="ACHVB7_002185"
     CDS             complement(8465..8716)
                     /gene="yqgD"
                     /locus_tag="ACHVB7_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR1914162.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8772..9926
                     /gene="metK"
                     /locus_tag="ACHVB7_002186"
     CDS             8772..9926
                     /gene="metK"
                     /locus_tag="ACHVB7_002186"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR1914163.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10362..11756
                     /gene="galP"
                     /locus_tag="ACHVB7_002187"
     CDS             10362..11756
                     /gene="galP"
                     /locus_tag="ACHVB7_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR1914164.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11833..12330
                     /locus_tag="ACHVB7_002188"
     CDS             11833..12330
                     /locus_tag="ACHVB7_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR1914165.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12425..13132
                     /gene="endA"
                     /locus_tag="ACHVB7_002189"
     CDS             12425..13132
                     /gene="endA"
                     /locus_tag="ACHVB7_002189"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR1914166.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13212..13943
                     /gene="rsmE"
                     /locus_tag="ACHVB7_002190"
     CDS             13212..13943
                     /gene="rsmE"
                     /locus_tag="ACHVB7_002190"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR1914167.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13956..14906
                     /gene="gshB"
                     /locus_tag="ACHVB7_002191"
     CDS             13956..14906
                     /gene="gshB"
                     /locus_tag="ACHVB7_002191"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR1914168.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15015..15578
                     /locus_tag="ACHVB7_002192"
     CDS             15015..15578
                     /locus_tag="ACHVB7_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR1914169.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15578..15994
                     /gene="ruvX"
                     /locus_tag="ACHVB7_002193"
     CDS             15578..15994
                     /gene="ruvX"
                     /locus_tag="ACHVB7_002193"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR1914170.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16169..17149)
                     /locus_tag="ACHVB7_002194"
     CDS             complement(16169..17149)
                     /locus_tag="ACHVB7_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR1914171.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17167..17871
                     /gene="yggS"
                     /locus_tag="ACHVB7_002195"
     CDS             17167..17871
                     /gene="yggS"
                     /locus_tag="ACHVB7_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR1914172.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17889..18455
                     /gene="yggT"
                     /locus_tag="ACHVB7_002196"
     CDS             17889..18455
                     /gene="yggT"
                     /locus_tag="ACHVB7_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR1914173.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18452..18742
                     /gene="yggU"
                     /locus_tag="ACHVB7_002197"
     CDS             18452..18742
                     /gene="yggU"
                     /locus_tag="ACHVB7_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR1914174.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIVALIN"
     gene            18750..19343
                     /locus_tag="ACHVB7_002198"
     CDS             18750..19343
                     /locus_tag="ACHVB7_002198"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR1914175.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19336..20472
                     /gene="hemW"
                     /locus_tag="ACHVB7_002199"
     CDS             19336..20472
                     /gene="hemW"
                     /locus_tag="ACHVB7_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR1914176.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         4997 a         5428 c         5288 g         4829 t
ORIGIN      
        1 tagcaaagga caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt
       61 tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac
      121 cgtccagtaa gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg
      181 tcagcggctt catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa
      241 taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca
      301 gcagcagccc taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt
      361 taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga
      421 ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta
      481 ccagcgcctc aatacactct ttgacgagag tattggcaat taacaccgcc gggccgataa
      541 cttcaaaacc cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa
      601 ccatctcaaa accggcaggt atgcgttttt ccagtgcctt atcaacatat tttatgacca
      661 gcccaccgag cggccccata atcattgagc caaggaacat cgggatctct gcgccgacga
      721 tcacaccaat agtacctatt ccgcccatga ccgcgccgcg tttaccgccg accagatgac
      781 caccggtaga accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg
      841 cgaaatgttc gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg
      901 caataaaagc accaatattg gggatgacca ttgccgtcaa aaagccccca aaagcctgga
      961 cctttgcacg agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc
     1021 cgcggctgat tatgtccgct aattgttttt ctgatgctgc tgttaagagt tgttcgagta
     1081 tttcttcttc gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat
     1141 cggcagcaga aaggccgatg agtaatttta ttggttcgtc attccccgga aaataaacac
     1201 cttgttcaag taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat
     1261 gaggcattgc cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt
     1321 taattgcctg aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga
     1381 aatcgatagc ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa
     1441 aatagtcact aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta
     1501 aaaataaaat actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg gtgttttgaa
     1561 aacagtgaat tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa
     1621 tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc
     1681 tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt
     1741 cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg
     1801 acgttgtcga cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg
     1861 aaggtggtca taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca
     1921 atacccgctt ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa
     1981 gccgcatcct gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg
     2041 ccttcggcag tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg
     2101 atgaagatca gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg
     2161 tttgccagtt gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc
     2221 gcggtcggac cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg
     2281 tcacacggac gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc
     2341 ggctggtgag tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc
     2401 tgacgctgtt tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc
     2461 aggaaggtgg aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg
     2521 gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt
     2581 gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg
     2641 aattccggca acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg
     2701 attttcgccg gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca
     2761 gacggcattt cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc
     2821 gcgtaagccg caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca
     2881 tcccactgcg catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt
     2941 tcgcgggtca gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg
     3001 gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca
     3061 gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg
     3121 tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag
     3181 ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc
     3241 agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg
     3301 tcgcccatga aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc
     3361 gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc
     3421 ggaccggtgg tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta
     3481 gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc
     3541 agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca
     3601 gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg
     3661 tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg
     3721 ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg
     3781 atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa
     3841 tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc
     3901 agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt
     3961 ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg
     4021 accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca
     4081 acggtactga ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa
     4141 gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt
     4201 caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg
     4261 acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg
     4321 tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc
     4381 gggctgatgg atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg
     4441 cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg
     4501 cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt
     4561 gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat
     4621 gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc
     4681 tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg
     4741 gcatccgccg aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa
     4801 agcctggtgg tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg
     4861 cacatttgtt accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta
     4921 cgggtggcat cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct
     4981 ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca
     5041 cttccactac gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag
     5101 cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat
     5161 ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt
     5221 gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc
     5281 cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca
     5341 gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat
     5401 aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag
     5461 cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg
     5521 cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa
     5581 aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc
     5641 acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac
     5701 cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc
     5761 agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat
     5821 tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac
     5881 aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg
     5941 tcacaataaa ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac
     6001 ttactcatct tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg
     6061 ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag
     6121 cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga
     6181 cagttctact tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc
     6241 gaacaggttg tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag
     6301 catcggcgga ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc
     6361 atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg
     6421 acgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc
     6481 ccatgcgtcc ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg
     6541 ttcctgcagt tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg
     6601 cacttcatgg cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa
     6661 ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca
     6721 ttcacgcaaa gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct
     6781 ttgcagcgcg cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc
     6841 cacgccgatg atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc
     6901 ggtgattacc gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat
     6961 gatgttgttg gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt
     7021 gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag
     7081 cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat
     7141 attcgccatc tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc
     7201 ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt
     7261 ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc
     7321 cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat
     7381 cttctcaatg accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat
     7441 atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc
     7501 cagtactgcc atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg
     7561 aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta
     7621 atcgccgtta tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa
     7681 acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc
     7741 acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca
     7801 cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt
     7861 gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg
     7921 tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc
     7981 gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc
     8041 gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca
     8101 accggtttct ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg
     8161 cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg
     8221 taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa
     8281 gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc
     8341 agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag
     8401 ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt
     8461 ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa
     8521 tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac
     8581 acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt
     8641 taatccatcc ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg
     8701 taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg
     8761 tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg
     8821 acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga
     8881 aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa
     8941 tcaccaccag cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg
     9001 gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg
     9061 gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg
     9121 cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag
     9181 cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca
     9241 ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca
     9301 aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga
     9361 aatcgctgca agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc
     9421 tgacttctgc caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga
     9481 tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac
     9541 gtcacggtgg cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct
     9601 acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa
     9661 ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg
     9721 gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc
     9781 gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag
     9841 catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc
     9901 tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc
     9961 gctggccttt ttcttttacc tcaccgcggt ggataggcat tcacgccgca tccggcaaaa
    10021 agctgcccgc acaataacat cattcttcct caacacgttt tatcgcaaat tatcatcact
    10081 actgaaaccg attacaccaa taacaccagc caaatatttg taatattttc atattattat
    10141 tcagttttca cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt
    10201 taatctatga tgatataact caattatttt catgcactta aaccataact aagataaatg
    10261 ttagtgtaag cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct
    10321 atcttaattc acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac
    10381 aggggcggtc aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat
    10441 tactctttgg cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat
    10501 tccagattac ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag
    10561 tcggtgcggt gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga
    10621 tcggcgcaat tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca aacgttgaag
    10681 tactgattct ttcccgcgtt ctactggggc tggcggtggg tgtggcctct tataccgcac
    10741 cgctgtacct ctctgaaatt gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc
    10801 agttgatgat cactatcggg atcctcggtg cttatctttc tgataccgcc ttcagctaca
    10861 ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg
    10921 gtgtcttctt cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg
    10981 ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg
    11041 aaatccgtga aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaacagca
    11101 acttccgccg cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga
    11161 tgaacgtcat catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca
    11221 cagagcaaat gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg
    11281 caatcggcct tgttgaccgc tggggacgaa aaccaacgct aacgctgggc ttcctggtga
    11341 tggctgctgg catgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg
    11401 cgcagtattt cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg
    11461 gtccgctgat ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca
    11521 tcacctgctc cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga
    11581 ccatgctcaa cacgctgggt aacgccaaca ccttctgggt gtatgcggct ctgaacgtac
    11641 tgtttatcct gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata
    11701 ttgaacgtaa cctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc
    11761 ctcaggcttc ctcccgtcgc ggaggaagcc gcctcttgca gtcatctttt cttcgctcta
    11821 tcctctgccg ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgc
    11881 ggcctgcggg aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca
    11941 aaactctctt acacccagcg cggaacctcc gccggaacag cctggctgga aagctatgaa
    12001 atccgcctca atcccgtttt gctgttggaa aacagtgaag cttttattga agaagtggta
    12061 ccgcacgaac tggcacattt gctggtatgg aaacatttcg gccgcgtagc gccacatggc
    12121 aaagagtgga agtggatgat ggaaagcgtg ctgggtgttc ccgcccgtcg tacgcatcag
    12181 ttcgaactgc aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat
    12241 cagcttaccg tacgccgcca taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt
    12301 cactgcggtg aacagctggt tgcgaaataa ccatctgaac tatcaggaac tttcctgatc
    12361 tggctgattg cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta
    12421 agtgatgtac cgttatttgt ctattgctgc ggtggtactg agcgcagcat tttccggccc
    12481 ggcgttggcc gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca
    12541 cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg
    12601 cgttgttgat ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg
    12661 cgtagagtgg gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc agtgctggca
    12721 ggacggtgga cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg aaagcgatat
    12781 gtataacctg cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag
    12841 ccagtggaat ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga
    12901 aaaagctgcc gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat
    12961 gcgcgaccaa tacaacctga cactctctcg ccagcaaacg cagctgttca acgcatggaa
    13021 caagatgtat ccggttaccg actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca
    13081 aggcaatcat aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact
    13141 agcgggattc tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg
    13201 gatttttaac tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc
    13261 acatcgcgct ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc atggggccgg
    13321 ggcaggcgtt gcaattgttt gacggtagca accaggtctt tgacgccgaa attaccagcg
    13381 ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc
    13441 cgctgcatat tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc
    13501 agaaatcgat cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg
    13561 ttaaactgga tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg
    13621 ctgcctgtga gcagtgtggt cgtaaccggg tgccggaaat ccgtccagcg atggatctgg
    13681 aagcctggtg tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta
    13741 acagcatcaa tacgttgccg ttaccggttg aacgcgtccg cctgctgatt ggcccggaag
    13801 gcggtttatc ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt
    13861 tgggacctcg cgttttgcgt acagagacaa ctgcgctcac cgccattacc gcgctacaag
    13921 tacgatttgg cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg
    13981 accccatcgc aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac
    14041 agcgtcgtgg ttacgaactt cactatatgg agatgggcga tctgtatctg atcaatggcg
    14101 aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa ctacgaagag tggttttcgt
    14161 tcgtcggtga acaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc
    14221 cgccgtttga taccgagttt atctacgcga cctatattct ggaacgtgcc gaagagaaag
    14281 ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg
    14341 cctggttctc tgacttaacg ccagaaacgc tggttacgcg caataaagcg cagctaaaag
    14401 cgttctggga gaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt
    14461 cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg
    14521 agaatggcac tcgctactgc atggcacaaa attacctgcc agccattaaa gatggcgaca
    14581 aacgcgtgct ggtggtggat ggcgagccgg taccgtactg cctggcgcgt attccgcagg
    14641 ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg
    14701 aaagtgactg gaaaatcgcc cgtcagatcg ggccgacgct gaaagaaaaa gggctgattt
    14761 ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct
    14821 gtattcgtga gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca
    14881 tcgaagcacg tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct
    14941 gagtgacagc gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa
    15001 cagaacctct gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg
    15061 atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg
    15121 ggatcatcgt caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga
    15181 agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg
    15241 gtccgctggc tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca
    15301 gcattcgcat ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg
    15361 gcaccgataa acaaccgtct gacgtattgg tggctctagg ttatgcctcc tgggaaaaag
    15421 gtcaactgga acaagaaatt ctcgataacg cgtggctaac ggccccggcg gatctgaata
    15481 ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg
    15541 atattctcac catgcctggt gtggcagggc acgcctgatg agtggaacct tactcgcctt
    15601 cgacttcggc accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcactgctcg
    15661 ccctttgcct gcaattaaag cacaggacgg tacgccggac tggaacctca tcgaacgttt
    15721 actgaaagag tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac
    15781 cgagcaacca ttgactgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg
    15841 tgttgaagta aagctccatg acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt
    15901 tgaacagggc ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat
    15961 tattctcgaa agctatttcg agcagggata ttaaggcgat ttaaaagcct ggcggatagt
    16021 cgttaccgcc atcttgccgg atgcggcgtg aacgcctatc cggcctacat gtggcagcga
    16081 ttgtaggtct gataagacgc gcaaacgtct catcagacgt ttattgcctt tgtaggcccg
    16141 ataagcttgc acatcgggca tggcaacgtc acaaacgccc ttcccccact cgctgctgaa
    16201 aactctgctg aaacgttatc atccccacct gctgcccggt ttgaataaca tgcggtaact
    16261 ggtgggtttt cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa
    16321 acagcgccac gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta
    16381 aactacctgc cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca
    16441 gtcgctcaac tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg
    16501 tttctgccgc cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa
    16561 tcacatcagg atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca
    16621 aaccaatttc ccgcttctgg atcaaacatc gctggctggc atagatatat tcaacaggat
    16681 cttccagcgt cagaatatgc gcatcggcat gttgattgag atagccaacc atcgccgcca
    16741 gcgtggtaga tttgctgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcactct
    16801 tgagtaattc cggcaatacc gttggtgcgc caagctgttc gagctgcggg cagtgcgaag
    16861 gtaacagccg taacgccagc gaaatgccat gccgttgcgc aaatgcgctg ccacgcaatc
    16921 gctggttttc cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc
    16981 gctgatcgtc atccagccac tcccgcagta gctcttcgac gtccggcgcc tcaaacggcg
    17041 cagcttccat tagcccgcga atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat
    17101 ccgagacgtt atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct
    17161 cggaaaatga acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct
    17221 gcaacgcgtt gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa
    17281 cctgcgagcg ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac
    17341 gttcaggaag gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa
    17401 tggcatttta ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac
    17461 tggtgtcata ccatcgaccg tttgcgcatt gctacccgcc tcaacgatca gcgcccggca
    17521 gaacttcccc ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc
    17581 gggattcaac tggcagaact ggacgagctg gcagcggcgg tcgctgaact accgcgttta
    17641 cgtctgcgcg ggctgatggc aatccctgcg cctgagtcag aatatgtaag gcagtttgaa
    17701 gttgcacgcc aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac
    17761 acgctctctc tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg
    17821 gttcgtatcg gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa
    17881 ggaacgccat gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg
    17941 tgctgttatt acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac
    18001 agtttgtagt gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa
    18061 tgggccccat tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca
    18121 tcgtgctgtt taaagtggtg accttcctgc caatcatctg gattgctggt ttactgattc
    18181 tgctgaaaac catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct
    18241 gggtaagcca gggtcgtagt ccgattgaat acgtgctgat tcagctggcc gatccgctgc
    18301 tgcgcccgat tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc
    18361 tcgttctgct gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa
    18421 acatgctgct gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg
    18481 tctggtttta cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca
    18541 tggcgacgaa gttaaagtcg ccattaccgc gccgccggtt gacggccagg gcaacagtca
    18601 tctggtgaag tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa
    18661 aggcgaactt ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga
    18721 aatcgtggcg ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg
    18781 ccggtaaagt gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc
    18841 aaacagacct cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga
    18901 ttctgaaagc gcgctatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg
    18961 gtctggcggt agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt tattccggtg
    19021 aagacgcgac cgatcaaaag aatctgcaaa aactgttgga aacactgaaa gacgtaccgg
    19081 acgaccaacg tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc
    19141 cgactccgct ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa ccagcgggca
    19201 ctggtggctt tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg
    19261 aactgacccg cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg aaactgctgc
    19321 tggacgcttt acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg
    19381 tgcgtgcaga aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg
    19441 cacgacgatt atgttcagca tctgctttgc gatctggaca atgatgtggc ttacgctcag
    19501 ggccgtgaag taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg
    19561 gcgatgcaaa cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa
    19621 attactatgg aagcgaaccc tggaacggta gaagccgatc gctttgtcga ttatcagcgt
    19681 gctggtgtga accgcatctc tattggtgtg cagagtttta gcgaagaaaa gctgaaacga
    19741 cttgggcgta ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg
    19801 gggctgcgta gctttaacct cgatttgatg catggactac cggatcaatc actggaagag
    19861 gcgcttggcg atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa
    19921 ctgaccatcg aacccaatac gctgtttggt tcgcgaccac cggtgctgcc ggacgatgac
    19981 gcgctgtggg atattttcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa
    20041 tatgaaactt ccgcttacgc caaacccggt tatcagtgcc agcacaatct caactactgg
    20101 cgatttggtg actacatcgg tattggctgc ggcgcgcacg gcaaagtcac cttcccggat
    20161 gggcgcattc tgcgtaccac taaaacgcgt catcctcgtg gttttatgca aggaaggtat
    20221 ctggaaagcc agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc
    20281 ttccgtctgc tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa
    20341 gatgtgattc gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg
    20401 gattactggc agataaccga gcacggtaaa ttattcttaa actcacttct tgaattattc
    20461 ctcgctgagt aatcattaat gcgcctgcgg gcgcattaat cacttctttc ccccccgtta
    20521 attaacttcg gcaaaatata tt
//
LOCUS       DAZPJR010000064        20400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPJR010000064 DAZPJR010000000
VERSION     DAZPJR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.065"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHVB7_002200"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHVB7_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR1914177.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="ACHVB7_002201"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="ACHVB7_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHVB7_002202"
     CDS             complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHVB7_002202"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR1914178.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHVB7_002203"
     CDS             4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHVB7_002203"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR1914179.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHVB7_002204"
     CDS             6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHVB7_002204"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR1914180.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHVB7_002205"
     CDS             6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHVB7_002205"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR1914181.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHVB7_002206"
     CDS             7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHVB7_002206"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR1914182.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8330..10048
                     /locus_tag="ACHVB7_002207"
     CDS             8330..10048
                     /locus_tag="ACHVB7_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR1914183.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSHSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHVB7_002208"
     CDS             10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHVB7_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR1914184.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHVB7_002209"
     CDS             10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHVB7_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR1914185.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11359..12672
                     /gene="gltP"
                     /locus_tag="ACHVB7_002210"
     CDS             11359..12672
                     /gene="gltP"
                     /locus_tag="ACHVB7_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR1914186.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHVB7_002211"
     CDS             complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHVB7_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR1914187.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHVB7_002212"
     CDS             complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHVB7_002212"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15262..15264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR1914188.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHVB7_002213"
     CDS             complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHVB7_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR1914189.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHVB7_002214"
     CDS             complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHVB7_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR1914190.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19393..>20400)
                     /gene="mdtN"
                     /locus_tag="ACHVB7_002215"
     CDS             complement(19393..>20400)
                     /gene="mdtN"
                     /locus_tag="ACHVB7_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR1914191.1"
                     /translation="PRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVV
                     PEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVD
                     AQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADL
                     NAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSV
                     GQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSI
                     GYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEP
                     Q"
BASE COUNT         4555 a         5435 c         5601 g         4809 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2221 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc
     2281 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac
     2341 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2401 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2461 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2521 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg
     2581 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2641 cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2701 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2761 atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag
     2821 cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc
     2881 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     2941 cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc
     3001 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3061 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg
     3121 gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3181 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3241 ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggccagcg ggccgtacag
     3301 caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg
     3361 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3421 atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc
     3481 ttcggcgttc atcttttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca
     3541 ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac
     3601 atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg
     3661 aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3721 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc
     3781 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3841 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3901 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     3961 gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4021 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt
     4081 tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt
     4141 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4201 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4261 cttgtagggt gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4321 tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa
     4381 tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcattg
     4441 ctgttgaaaa agaggaagat actaactaac tctaaagtgg tattttacat gcacttacaa
     4501 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca
     4561 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gtaacaatgg
     4621 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4681 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
     4741 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
     4801 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
     4861 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
     4921 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
     4981 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
     5041 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
     5101 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
     5161 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
     5221 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
     5281 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
     5341 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
     5401 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
     5461 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
     5521 gaaggcaaac tctataccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
     5581 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
     5641 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
     5701 gcgaaagcag cgctggatgc aggcgcgacg gaagctgaaa tgaagccaat tcaggacgat
     5761 atccgtcatg cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac
     5821 gcaccggaag aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc
     5881 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
     5941 atctcaacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
     6001 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac
     6061 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
     6121 atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga
     6181 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta
     6241 cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta
     6301 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
     6361 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
     6421 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg
     6481 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
     6541 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
     6601 ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt
     6661 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
     6721 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
     6781 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
     6841 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
     6901 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
     6961 ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga
     7021 tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat
     7081 cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
     7141 cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgtg ttgaggcttg
     7201 cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact
     7261 gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
     7321 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
     7381 gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
     7441 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
     7501 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
     7561 atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
     7621 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg
     7681 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
     7741 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
     7801 acgctgatgc tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg
     7861 ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc
     7921 atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg
     7981 cattccacgg aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc
     8041 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg
     8101 ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
     8161 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
     8221 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt
     8281 ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt
     8341 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
     8401 gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat
     8461 cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
     8521 tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agccatagcc tgctgtcgtg
     8581 ggaattcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct
     8641 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
     8701 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt
     8761 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
     8821 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
     8881 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc
     8941 ggccagcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg
     9001 gatcatcctc ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg
     9061 ggatccggtg gaaaatgcct ctttattacc ctggctttct gccactgcgc tgctgcacag
     9121 tttatccctg acacgccagc gggggatttt ttgccactgg tcgctgttgc tggcgatagt
     9181 tactctgatg ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
     9241 tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
     9301 tagccttgcg tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
     9361 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
     9421 gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggtctgc tgggctgggg
     9481 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
     9541 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgcgcagc ttccggcgct
     9601 ggtcgctcat gcgggcgtgc tgttatttgc cgcagggatc gtggtttcca gcgtcagccg
     9661 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
     9721 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
     9781 ggcgctgttt gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga
     9841 agcacgccgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
     9901 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag
     9961 cggtgtgcgc tggatctggg gtggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    10021 atggcggggg aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct
    10081 gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa caacagcaac
    10141 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg
    10201 aatccaatgc gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg
    10261 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    10321 ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat
    10381 tgctgatggc actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc
    10441 ggtgaaaatg ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct
    10501 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    10561 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg
    10621 cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa
    10681 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc
    10741 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    10801 ccagacttgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    10861 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    10921 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    10981 taatatggca aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    11041 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    11101 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    11161 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    11221 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    11281 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    11341 attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt
    11401 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    11461 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    11521 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    11581 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    11641 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    11701 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    11761 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    11821 atggcgaaag gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt
    11881 tcttccctgc ccgcgacgca tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa
    11941 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    12001 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    12061 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    12121 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    12181 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    12241 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    12301 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    12361 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    12421 gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt
    12481 ccgctggaag gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact
    12541 gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    12601 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact
    12661 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtga atgcctgatg
    12721 cgacgcttgc cgcgtcttat caggcctacc ttaaccgttg gttaattttt caaactcttc
    12781 acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg ccttttgctt
    12841 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    12901 ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    12961 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    13021 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatagcct ttggataatc
    13081 cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac cttctttact
    13141 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    13201 attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc tgtcggcggc
    13261 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    13321 aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc tgccaggctc
    13381 ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    13441 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    13501 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    13561 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    13621 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    13681 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    13741 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    13801 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    13861 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    13921 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    13981 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    14041 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
    14101 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
    14161 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
    14221 ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg gttcaactgc
    14281 tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    14341 cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    14401 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct ccgcgtcagt
    14461 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg ctgcgcggtg
    14521 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    14581 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    14641 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    14701 cgcatgcggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    14761 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    14821 ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    14881 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    14941 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggc ccatcgcatt
    15001 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    15061 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    15121 gtgattcgcc acgattgggt gggaatcagc cgggttgtac ccgaaaacga acactaaatc
    15181 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    15241 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    15301 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    15361 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggt
    15421 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    15481 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    15541 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    15601 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    15661 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    15721 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
    15781 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    15841 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    15901 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    15961 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcagtggcc
    16021 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg ggtgaagcgc
    16081 gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg cagacgcgtg
    16141 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
    16201 atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    16261 ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa taaggtatcc
    16321 aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta gaacagcgcc
    16381 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
    16441 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gactcgtggt
    16501 aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa tgcacgcaga
    16561 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
    16621 atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc cactttactc
    16681 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
    16741 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    16801 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc aaccgctgag
    16861 cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt ggcttccgta
    16921 tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct taaaaagccg
    16981 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    17041 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    17101 gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctgcgggtc attgagttgt
    17161 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    17221 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tgcgtaccaa ggcacagccg
    17281 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    17341 atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg gctgtccagt
    17401 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    17461 tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    17521 ccagaccggc agcgtatttt tccagggcgt cggcaaactg acctgctctg tccggctgta
    17581 gtgcgttatc cagcgcctgc gccgaattcc aggtggcatc ccaggcgtga aggatatcgc
    17641 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    17701 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    17761 gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    17821 gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    17881 cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca atgatgatac
    17941 ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg ttttcgagcg
    18001 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    18061 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    18121 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    18181 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagagacga
    18241 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg taaaaggtgt
    18301 aacagatcaa acaggcgagc aatgttttta ccgcgtagcg tatatagtct ggattggtaa
    18361 aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta ttcgggttca
    18421 tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc cgtgccgcca
    18481 ccgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca gcaacggcat
    18541 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    18601 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    18661 cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg agacaaaaga
    18721 cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    18781 ggctgtctgt caggtgacta atggcatcat caagccgatc attaagcgcc tgatgcattt
    18841 gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    18901 agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga tagtcgagca
    18961 tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag accagtccca
    19021 gccgatgggt gcgcatcaaa aacatgcggc ccatcaggat cggcccggcg atgatcaatc
    19081 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    19141 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    19201 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    19261 gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg ccgggtcgcc
    19321 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac catcggtaat ggcagggagt
    19381 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    19441 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    19501 gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    19561 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cactcatcag
    19621 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    19681 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    19741 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    19801 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    19861 cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    19921 aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    19981 aaaaccctct ttcagtaatg gctcggtacg gcgtaatgta tctgtggcct gtttcgcggc
    20041 ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt gcgcatcaac
    20101 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    20161 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttgactgc
    20221 ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca ccacatcaat
    20281 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    20341 gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt tactgcgagg
//
LOCUS       DAZPJR010000065        20299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPJR010000065 DAZPJR010000000
VERSION     DAZPJR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.066"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..70
                     /locus_tag="ACHVB7_002216"
     CDS             <1..70
                     /locus_tag="ACHVB7_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFR1914192.1"
                     /translation="ILKTTDYLRIAQPELLNFRRTA"
     gene            complement(71..175)
                     /gene="yoaI"
                     /locus_tag="ACHVB7_002217"
     CDS             complement(71..175)
                     /gene="yoaI"
                     /locus_tag="ACHVB7_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR1914193.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            345..791
                     /locus_tag="ACHVB7_002218"
     CDS             345..791
                     /locus_tag="ACHVB7_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR1914194.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(748..1569)
                     /gene="nimR"
                     /locus_tag="ACHVB7_002219"
     CDS             complement(748..1569)
                     /gene="nimR"
                     /locus_tag="ACHVB7_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR1914195.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            1666..2847
                     /gene="nimT"
                     /locus_tag="ACHVB7_002220"
     CDS             1666..2847
                     /gene="nimT"
                     /locus_tag="ACHVB7_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR1914196.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            2902..3249
                     /locus_tag="ACHVB7_002221"
     CDS             2902..3249
                     /locus_tag="ACHVB7_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR1914197.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3271..3525)
                     /gene="yoaF"
                     /locus_tag="ACHVB7_002222"
     CDS             complement(3271..3525)
                     /gene="yoaF"
                     /locus_tag="ACHVB7_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR1914198.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            3708..4733
                     /gene="dgcP"
                     /locus_tag="ACHVB7_002223"
     CDS             3708..4733
                     /gene="dgcP"
                     /locus_tag="ACHVB7_002223"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR1914199.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            4766..4864
                     /locus_tag="ACHVB7_002224"
     CDS             4766..4864
                     /locus_tag="ACHVB7_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR1914200.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            4867..4941
                     /gene="yoaJ"
                     /locus_tag="ACHVB7_002225"
     CDS             4867..4941
                     /gene="yoaJ"
                     /locus_tag="ACHVB7_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR1914201.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(5000..5248)
                     /locus_tag="ACHVB7_002226"
     CDS             complement(5000..5248)
                     /locus_tag="ACHVB7_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1914202.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(5396..5578)
                     /locus_tag="ACHVB7_002227"
     CDS             complement(5396..5578)
                     /locus_tag="ACHVB7_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR1914203.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(5582..5941)
                     /gene="yeaR"
                     /locus_tag="ACHVB7_002228"
     CDS             complement(5582..5941)
                     /gene="yeaR"
                     /locus_tag="ACHVB7_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR1914204.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(6114..6752)
                     /gene="leuE"
                     /locus_tag="ACHVB7_002229"
     CDS             complement(6114..6752)
                     /gene="leuE"
                     /locus_tag="ACHVB7_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR1914205.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(6879..7802)
                     /gene="dmlR"
                     /locus_tag="ACHVB7_002230"
     CDS             complement(6879..7802)
                     /gene="dmlR"
                     /locus_tag="ACHVB7_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR1914206.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            7905..8990
                     /gene="dmlA"
                     /locus_tag="ACHVB7_002231"
     CDS             7905..8990
                     /gene="dmlA"
                     /locus_tag="ACHVB7_002231"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR1914207.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVAIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            9241..10851
                     /gene="yeaV"
                     /locus_tag="ACHVB7_002232"
     CDS             9241..10851
                     /gene="yeaV"
                     /locus_tag="ACHVB7_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR1914208.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            10883..12007
                     /gene="yeaW"
                     /locus_tag="ACHVB7_002233"
     CDS             10883..12007
                     /gene="yeaW"
                     /locus_tag="ACHVB7_002233"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR1914209.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            12063..13028
                     /gene="yeaX"
                     /locus_tag="ACHVB7_002234"
     CDS             12063..13028
                     /gene="yeaX"
                     /locus_tag="ACHVB7_002234"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR1914210.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(13082..14197)
                     /gene="rnd"
                     /locus_tag="ACHVB7_002235"
     CDS             complement(13082..14197)
                     /gene="rnd"
                     /locus_tag="ACHVB7_002235"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR1914211.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(14279..15964)
                     /gene="fadD"
                     /locus_tag="ACHVB7_002236"
     CDS             complement(14279..15964)
                     /gene="fadD"
                     /locus_tag="ACHVB7_002236"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR1914212.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(16169..16750)
                     /gene="yeaY"
                     /locus_tag="ACHVB7_002237"
     CDS             complement(16169..16750)
                     /gene="yeaY"
                     /locus_tag="ACHVB7_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR1914213.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(16790..17485)
                     /gene="tsaB"
                     /locus_tag="ACHVB7_002238"
     CDS             complement(16790..17485)
                     /gene="tsaB"
                     /locus_tag="ACHVB7_002238"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR1914214.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(17543..19453)
                     /locus_tag="ACHVB7_002239"
     CDS             complement(17543..19453)
                     /locus_tag="ACHVB7_002239"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR1914215.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            19585..19929
                     /locus_tag="ACHVB7_002240"
     CDS             19585..19929
                     /locus_tag="ACHVB7_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1914216.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4934 a         5248 c         4949 g         5168 t
ORIGIN      
        1 tattttgaaa accacggatt atttgcgcat tgcgcaacca gaactgctca atttccgccg
       61 cactgcgtaa ctagcctgtc cgttcaataa gcagaacgga caaaaccagc acaacagcaa
      121 tggcaaaaaa cgatgacgtg ataatcagtg tctcgacgaa catttgatcg ttcatagcat
      181 gcccccggga gatagctgtt ttcacgttat tgttagcgtg cacaaatggc agtttgatga
      241 caattcgcct gattttttat gcaaaaaacg taaagatttt ctacttcctt cctgcagcaa
      301 acgtaaagta agcaggctta ttattttttg gcaaggaaac cacgatgttt gatgtcactt
      361 tgctgatcct gctcgggtta gctgcgctgg gctttatcag tcataacacc actgtcgccg
      421 tttcaattct ggtgttgatc attgtccgcg tcacaccgtt aagcaccttt ttcccgtgga
      481 ttgaaaaaca ggggctaagt atcgggataa tcattctgac gattggcgtt atggccccca
      541 tcgccagcgg gacgctaccg ccctcaacct tgattcactc gtttctgaac tggaaatcgc
      601 tggtggcaat tgcagtaggc gtgattgtct cctggctggg tgggcgcggc gtgaccttaa
      661 tgggcagcca gccgcaactg gtcgccgggc tgctggtagg cactgtttta ggcgtggcgc
      721 tgtttcgcgg cgtaccggtc ggcccgctta ttgccgcggg tctggtttcg ctgattgtgg
      781 ggaaacagta gttaatcccg caatatatcg gcctggcgtt tgccccaggc ctttcttaaa
      841 catagtaata aaggccgttg ttgaatcata ccccagcgta tgagcaactt tctgtacagt
      901 atcgcctttc actaatccct gaagtgccat aatcagctgt aactgctgac gccactggcg
      961 aaaactcaac cccgtctctt ttactatcag tcgcgcaaga ttgcgttcac tcatcgcaaa
     1021 aaagccagcc cattgcccta atgaccccca ctcgacgggc cctttcgcca tcatctccac
     1081 catcgtgcgg attttaggat gagaagaaac gggcaggtgc agctgttgtt gtggctgctg
     1141 agggagttca tcaaacagga cctgaattaa tctgcgggtc atcggctcag cacgctgagt
     1201 cgtcgtcctg tttgccaggg ttaaaatcaa ttcccggcat aagggcgata ttttcaatgt
     1261 gcaacatgtt gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc
     1321 gttagctgtc acttgattgc tatgttccac cccgcccggt atccaaaccg catattgcgg
     1381 cggcaccatc cataaagcat tttccaccgt acaggtaatt gcaccatgta atgctagtat
     1441 cagctgtcct ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat
     1501 acaaaatgcg accgcggctt catggtgtcg gtcgggttca tagccattga gattcaacct
     1561 gtgcatcatt ttgtccgaat ttagcgataa tttgtcattt tagcttgatt caacataaca
     1621 ataaaaacgg taaggtacag cctcgtttgt aacaatgaga agcatatgac ctgttcaact
     1681 tcattaagcg gcaaaaacag gattgtcctt atcgctggca ttctgatgat tgccacaaca
     1741 ttacgcgtca cctttatcgg cgcagcaccg ttactggata cgattcgttc cgcttactcg
     1801 ctgacgacag cgcaaaccgg cttattgacc accctgccat tattggcctt tgcgctaatc
     1861 tcacctttgg ccaccccggt agcgcgacgt tttggtatgg aacgtagcct gtttgccgcg
     1921 ttacttttga tctgtgctgg tatcgcaatt cgctctctcc cttcgcctta cttattattt
     1981 ggcggtacag cggtcattgg cggtgggatt gcattaggca atgtcttact gccaggatta
     2041 attaaacgcg atttccctca ttccgtcgcc agacttaccg gcgcatattc cctgacaatg
     2101 ggagctgcag cggcactggg atcggctatg atagtgccgc tggctttgaa cggttttggc
     2161 tggcaaggcg cgttgctcat gctgatgtgt tttcctctgc tggctctttt tttatggctg
     2221 ccacagtggc gaagccaaca acatgcaaat ttgagtacct cgcgcgcctt acatactcgg
     2281 ggtatctggc gttcgccgct tgcctggcag gtaacattgt ttcttgggat caactcactg
     2341 atctattacg tgattattgg ctggcttccg gcgatcctca tcagtcacgg ctatagcgaa
     2401 gcacaggcgg gttcactgca tggtttgctg caactagcca cagcagctcc cggtttgctg
     2461 atccctcttt tcttacatca tgtgaaagat cagcgtggta ttgcagcgtt cgttgccttg
     2521 atgtgcgcag tgggcgctgt tgggctctgc tttatgccag cgcacgcgat cacctggact
     2581 ctgcttttcg gttttggttc cggcgcaaca atgatactgg ggttgacgtt cattggtctt
     2641 cgggcaagtt ctgcgcatca ggcggcggca ctctcgggga tggcacaatc cgtcgggtat
     2701 ttgctggcag cctgtgggcc gccgctaatg ggtaaaatac acgatgctaa cggtgactgg
     2761 tccgtaccac tgatgggtgt tgcaatactt tcactactga tggcgatttt cggactttgc
     2821 gccgggagag acaaagaaat tcgctaatat ccggtgctat agtgacgtga caaatcatgc
     2881 gtgaaaggga gaacaaacac gatgaatatt cagtgcaaac gcgtttatga tccggctgaa
     2941 cagagcgatg gttatcgcgt actggtcgac cgcctctggc cgcgcggtat caaaaaaacc
     3001 gatttagccc ttgatgagtg ggataaagaa atcacgccgt caacggaact gcgcaaagcc
     3061 tttcacggcg aagtcgtcga ttttgcaacc tttcgcgagc aatatcttgc agaactggcg
     3121 caacacgagc aagaaggaaa gcggctggcg gacatcgcca aaaaacagcc gctgaccctg
     3181 ctctacgcag caaaaaacac cacgcagaac catgcgctgg tgctggccga ctggctacgt
     3241 agcttgtgat tttagtacag catccggcgg ttatttttca ccagccggat ggtcccgtcg
     3301 ccacaatgcc cattcatcaa tcgtttcacc gcccggtaat ttgcaattgt tgctgacccc
     3361 ttgcgctgtc gacactggaa tgagcgtccc gcccttctgc tggcaataga ccgacgccgg
     3421 atttgccata ccaatctgcg gcggtttagg tacttctggc tgagaagagg ttgaacaacc
     3481 agccaggacc agcaagcaag gcaaagcaaa actgataatt ttcatttatt gatctcactc
     3541 atttatctac gcttagagta tcgcggtatc gttttgtttt gcagcactat ttttattaca
     3601 ttcactcaaa acatattacg tcttgtttca tctttgttgg tgatgtttta tcatgcctgc
     3661 aaagattaaa taatcagcat ttacccgccg tatcctggag ttgtttcgtg tcagatcaga
     3721 ttatcgcccg cgtctcgcaa tcccttgcca aagaacagtc actggaaagt ctggtccgac
     3781 agcttctgga gatgctggaa atggtcactg atatggaatc aacctacctg accaaagtgg
     3841 atgtcgaagc gcgcctgcag catattatgt ttgcccgtaa cagccagaaa atgcacatcc
     3901 cggagaattt taccgtctcg tgggattact cgttatgcaa acgcgccatt gatgaaaact
     3961 gctttttcag cgatgaagtc cctgaccgtt ggggcgactg tattgcggca cgcaatcttg
     4021 gcatctccac atttctgagc acaccaattc acttaccgga tggatcattc tatggcacgc
     4081 tttgcgccgc cagcagtgag aagcgccagt ggagtgaacg cgcggaacag gttttacagt
     4141 tattcgccgg actgattgca caatatattc aaaaagaggc actggttgaa cagttgcgcg
     4201 aagccaatgc tgcgctgatt gcgcaatcgt ataccgactc gttaaccggg ttgccgaatc
     4261 cgcgggcgat ttttgaaaat ctgacgacgc tgttttctct cgcccggcat cttaaccata
     4321 agataatgat cgcgtttatc gatctggata acttcaaatt aatcaatgat cgttttggtc
     4381 ataatagtgg cgatctgttt ctcattcagg ttggcgagcg ccttaatacg ctccagcaaa
     4441 atggcgaagt tattggtcgt ctcggcggtg atgagttttt ggttgtttca ctaaacaacg
     4501 agaatgcgga tatttcgtcg ctgcgagaac gcattcagca gcaaatacgt ggagaatatc
     4561 acttaggtga tgttgatttg tattatcccg gtgccagtct tggcatagta gaagtcgatc
     4621 ctgaaacaac cgatgcagac agtgccctgc atgctgccga tattgcgatg tatcaggaga
     4681 aaaaacacaa acagaaaaca ccttttgtcg cgcattcagc gccacattgc tgaggcgtat
     4741 tcacatcctt ttgattggtg ataacatgcg aatcggtatt atttttccgg ttgtaatctt
     4801 tattacagcg gtcgtatttt tagcatggtt ttttattggc ggctatgctg ccccgggggc
     4861 ataaagatga aaaaaacaac gattattatg atgggtgtgg cgattattgt cgtactcggc
     4921 actgagctgg gatggtggta acgtcacctc taaaaaatag caaaggctgc ctgtgtgcag
     4981 cctttgtgca atttaagcgt taacttttaa tcttcctgta gataaatagc acgacaatcg
     5041 caccaataac ggcaaccacg aagctgccaa aattgaagcc atcgacttta ccaaagccaa
     5101 acagcgtgct gatccatccg ccgactacgg caccgactat ccccagcagg atagtcataa
     5161 agaatccacc accatcttta cctggcatga tccacttcgc cagaataccg gcaataagcc
     5221 caaaaataat ccatgacaga atgcccattg tttcctcact tatctgtttt gcattagcgg
     5281 gttagtcgct gataaaaagc atagcacaac atcgggaggg caagatttgt gacgagcatc
     5341 acggaggatt tttttgcgat ggcgcagaaa ttgcgccatc aacgatcagt gataattacc
     5401 aaccacaaac atcatgttcg ttttccgtgt cataagaacg cacggtattc accagatctt
     5461 ttatcacttc agccgccact tctggcacca gcaaagtcat cggcgtctct gtttcataat
     5521 cgacagaaac gccattactg ttattggtga cggtcacggt atacgttgct ttgcccatga
     5581 ttcatttccc gttatgaatg actttccgtt gttgcgcacc ttccatcagg acttcaggag
     5641 ccacgaagaa gtcaatgttg aaataagtat cgtcagtcat ggcttcaatg ttgtgccact
     5701 tttctggagg gaacacctca aactgccccg cttcgataag gatcacctga tcaggctctg
     5761 cactgtgttc atcagcgtag ccgagatatt tgaccgcccc atgcataacg gaaaggcgtg
     5821 ggtaaacccc cgggcgcgtt cctttatcaa gatgacgttc gaatattccg gcaggcgcag
     5881 tttgtttatt ccagaaaggc gttgagcgcg tatgaatata attctgtggg atttgcagca
     5941 tccttttccc tccttcggtg aatgcgctga aaacggttta ttccagccgt ttcagggtac
     6001 gcctgataat ttgcatttta aataccattt attggttact ttttagcacc atatcagaga
     6061 aaaatcaggg aggattacaa gtgggaagcc cgcgacaacg cgggctgaat gtatcaggat
     6121 tgcagcgtcg ccagtcgggc ggcgaaaccc acgaacatca aaccaatcag tgagttgcca
     6181 actttagcca gtttcttttt ggtacgtatg tactgcgtga caaaagcacc agaaataatc
     6241 aggaagctca aatagcagaa actcaccagt tccagcgtcg tcgccagaat aaagaatgaa
     6301 attcccgtat gtggggcatt aacatcgata aactgtacga aaaacgacac atagaacaaa
     6361 atggctttcg gattagtcag gctcaaaatt aacgcgcgtt taaaaatggc cccgtattgg
     6421 ggctcatcgg atttggcctc gttattttta cccttcaggg tcgcgtagag aattttactc
     6481 cccagataga gcaaataaaa cgcaccaaga taacgtacaa tgttgaataa tatcggggtg
     6541 gtcttaatta atgtcgccac tccagcccat gccagaaaca tcaatactgc atcgccaata
     6601 aatacaccgc aggccgcaag ataaccgcct ttcataccgc tactgacgct atttttgagt
     6661 acaaacaggg tatttggccc tggcaccaac acaataaaaa tggccccaac cagataggtc
     6721 cagtaattca gaaccccgta ttcagcgaac acattaacct ctttaattat ctttcgatca
     6781 tgcgcgatta aaggtgaata tgctaaccaa tctgtagcgg cttagaaagg agaaaatcag
     6841 gttttaaccc gatatcaacc cgataattga ataattaatc aggcatgctc cagtgaaaaa
     6901 ttcgggtagt gctcggcaaa atactggcgt aaaaattcta tcgttatccg cactttcgct
     6961 gacgtcgcca gccttgaaac ataaacggcc cagacgttcg ctggctggta atattccggt
     7021 agcacttgca ctaaatgacc actggcaatg ttttcgctaa catcccacca ggagcgcaga
     7081 gcaatcccct gcccatcgag acaccactga tggacaattt ctccatgatt agacgacagt
     7141 gggccggtga ctttaatcgc atggtggcct tctttattgc gtaattgcca gacaccaaaa
     7201 ggatgatcgc gctctttaat gaccaggcac ggcaatgcgg aaagatcggt taaatgtttc
     7261 ggcgcgccat gttgcgcaat aaattccggc gacgcgcaga gaatgcggta attagtcgcc
     7321 agtttgcggg cgattaaatt aggggcgatg tcatcgccaa tgcgaatatc gagatcaaca
     7381 ccttcattga ccaaatcgac caaccggtct tccacatcaa aacgtaattc cagttgcgga
     7441 tacgccttag ccagcgctga taacgccgga gccaccacct gtcgtccaaa accaaagctg
     7501 ctgataatac gcagcatccc ctgcggcacc tgacgcacgt cagaaagttc gtccatcatc
     7561 tgaccgacat cctgcaaaat ccgctgcgcc cattcataaa tccgctctcc ttcttcggta
     7621 atggtgacgc ggcgggtggt gcggtgtaac aacaccacgt ttagcgtttg ctccagcaag
     7681 gcgatgcgct tgctgacgaa cgccggtgaa acgcccagtt cttccgccac ggcggcaaaa
     7741 ccagcccggc gagccaccag cataaagacg cgcaaatcgt tcagcagcgg taaattattc
     7801 atgattcgtg ttttatgttt caccagttac ggggattaat tccttttcag tcaattatag
     7861 gatggtgatg ttgtcaattt tgatggtcag gaagtgagaa cccaatgatg aaaacgatgc
     7921 gtattgctgc gatcccggga gatgggattg gcaaagaagt ccttcctgaa gggattcgcg
     7981 tattacaggc tgccgctgag cgctggggct tcgacttgag ttttgagcaa atggagtggg
     8041 cgagctgcga gtattacagc catcacggta aaatgatgcc ggacgactgg catgagcaac
     8101 ttagccgttt cgacgccatc tattttggtg ccgtcggctg gccggatacc gttccggacc
     8161 atatttcgtt gtggggttcg ctgctgaaat ttcgtcgtga attcgaccag tacgtcaacc
     8221 tgcgcccggt tcgtctcttt cctggcgttc cctgcccgct ggcgggaaaa cagcctggcg
     8281 acatcgattt ttacgtggtc agggaaaaca ccgaaggcga atattcctcg ctcggcggta
     8341 gagtgaatga aggtacagag catgaagtcg ccattcagga atcggtattt acgcgtcgtg
     8401 gtgtcgatcg cattttgcgt tatgccttcg aacttgcgca aagccgccca cgtaagacgc
     8461 taacttctgc cactaaatca aacggtttag ccatcagcat gccgtactgg gatgagcgag
     8521 tggaagcaat ggccgagaat tacccggaga tccgctggga caagcagcat attgatattc
     8581 tctgcgcgcg ttttgtgatg cagccggaac gcttcgatgt ggtggtggcg tccaatttgt
     8641 ttggcgatat cctttccgat cttggcccgg cctgcaccgg caccattggc attgccccat
     8701 ccgccaacct gaatccggaa cgcactttcc cgtcgctctt cgagcctgtc cacggttccg
     8761 cgccggatat ctacgggaaa aatattgcta accctatcgc cacaatttgg gccggtgcga
     8821 tgatgctcga ttttctcggc aatggcgatg agcgtttcca gcaagcgcat aacggtattc
     8881 tggcagcgat tgaagaagtg attgctcacg ggccgaaaac accggatatg aaaggcagtg
     8941 ccaccacgcc acaggttgcc gacgcgattt gcaaaattat tttgcgttaa ggtcaaacca
     9001 gtttatttga accgcgtcac tgacgcggtt tttttattcg ttctttgcag ttaataacct
     9061 gcgtcatttc accttttatt gtttccgttt cgtgttttat ggctttccgt attcttaatt
     9121 gtttaattta tgtaacatgc aaataatcaa tttgttaaac acaacataca actcattacc
     9181 ctgcaaataa ttacaactgg caaaagccag agacgggctt ttatgcccag gagatgactc
     9241 atgatgagca atgttaagaa aaaagatgtg ccgctgataa gcatcagcct ggtggccatt
     9301 cttttcatcg cagctgcatt aagccttttc ccacaacaat cggccgacgc ggccaacaca
     9361 atatacactt ttgttacgcg tacgttaggt tccgccgtac aggtattggt tttgctggca
     9421 atgggactgg tgatttattt agccaccagt aaatacggca atattcgtct tggcgaagga
     9481 aaaccggaat acagcacgct ctcctggctg tttatgttta tttgtgccgg tttaggttct
     9541 tctacgcttt attggggggg tgctgaatgg gcctattatt atcaaacgcc tggattaaat
     9601 atcgcaccac gttcacaaca ggcactcgaa tttagcgtcc cttactcctt cttccactgg
     9661 ggcatcagca cctgggcaac ttatacgctg gcctcattaa tcatggctta tcactttcat
     9721 gtgcgtaaaa acaaaggtct gagtctttcc ggcattattg ccgcgattac cggcgttcgc
     9781 ccgcaaggcc catggggaaa actggttgat ttgatgtttc tgatcgccac tgtcggcgca
     9841 ctgactattt cccttgttgt caccgcagca acctttactc gtggactttc cgcgctgacc
     9901 ggtttacccg ataacttcac cgtgcaggca tttgtgatcc tgctttccgg cggcattttt
     9961 tgcctaagct cgtggattgg tatcaacaac ggtttgcaac gtctgagcaa aatggttggc
    10021 tggggcgcgt tcctgctgcc attactggtg ctgattgtcg gtccaaccga atttattacc
    10081 aacagcatca tcaatgccat cggcctgacc acgcaaaact tcctgcaaat gagcttattc
    10141 accgatccgc ttggcgatgg ttcatttacc cgcaactgga ccgttttcta ctggctgtgg
    10201 tggatctcat acccccctgg cgtagcaatg tttgtcaccc gcgtttcccg cggtcgtaag
    10261 attaaagaag ttatctgggg actgatcctc ggcagcaccg tcggttgctg gttcttcttc
    10321 ggcgtaatgg aaagctatgc cattcatcag tttatcaatg gcgtaatcaa cgtcccacag
    10381 gtgctggaaa cactgggcgg cgaaacagct gtacagcaag ttctgatgtc gttgccagcc
    10441 ggtaaattgt tcctcgccgc atacctgggc gtgatgatta ttttccttgc ctcgcatatg
    10501 gatgcagtgg cctacaccat ggcggcgacc agtacgcgta atctccagga aggtgacgat
    10561 cctgaccgtg ggctgcgtct tttctggtgc gtggtgatca ctctgatccc gctttccatc
    10621 ttgtttaccg gtgcttcgct ggaaacgatg aaaaccaccg tcgtgctcac agcccttccc
    10681 ttcctcgtca ttttactggt gaaagtcggc ggatttattc gctggctgaa acaggattat
    10741 gccgacattc cggctcatca agttgaacat tatctcccgc agacaccggt tgaagccctg
    10801 gaaaaaacac cagtgctccc tgcgggaacc gtattcaaag gcgacaactg agcgccatca
    10861 tcctaacgat aaaggtatcc ctatgagcaa tctgagccct gactttgtac tacccgaaaa
    10921 tttttgcgct aacccgcaag aggcgtggac cattcctgcc cgtttttata ccgatcagaa
    10981 cgcgtttgaa cacgaaaaag agaacgtctt cgccaaaagc tggatttgcg tcgctcacag
    11041 cagcgaactg gcgaatgcca atgattatgt gacgcgtgag atcattggcg aaagcatcgt
    11101 gctggtacgc ggtcgtgata aggttttgcg cgcgttctat aacgtgtgtc cgcaccgtgg
    11161 tcatcagttg ttgagcggtg aaggaaaagc aaaaaatgtg attacctgcc catatcacgc
    11221 atgggcattc aaactcgatg gcaatctggc ccatgcacgt aactgcgaaa acgtcgccaa
    11281 tttcgatagc gacaaagcgc aactggttcc ggtgcgtctg gaggaatatg ccggattcgt
    11341 cttcatcaac atggacccca acgccaccag cgtagaagat cagttacccg gtctgggcgc
    11401 gaaagtgctg gaagcctgcc cggaagtcca cgatctgaaa ctggcggccc gctttaccac
    11461 ccgcacgcct gccaactgga agaacattgt cgataactat ctcgagtgct atcactgtgg
    11521 tccggcgcat ccaggtttct ccgactccgt acaggttgat cgctactggc acaccatgca
    11581 cggtaactgg acgctgcaat acggtttcgc caaaccgtcc gaacagtcgt ttaaatttga
    11641 agagggtacg gatgcggcat tccacggttt ctggctgtgg ccgtgcacga tgctgaacgt
    11701 caccccgatc aaagggatga tgacggtcat ttatgaattc ccggtggatt ctgaaactac
    11761 cctgcaaaac tacgatattt acttcaccaa tgaagagtta accgacgagc aaaaatcgct
    11821 gattgagtgg tatcgcgatg tgttccgtcc ggaagattta cgtctggttg aaagcgtaca
    11881 gaaagggctg aaatcgcgtg gctatcgtgg tcaggggcgc atcatggccg acagtagcgg
    11941 tagtggcatt tccgaacatg gtatcgccca tttccataat ctgctggcgc aggtgtttaa
    12001 ggactaatga catcggcggc ggtattttcc gccgctgggc tgatttttga tggagtacag
    12061 caatgtcaga ctatcaaatg tttgaagtac aggtgagtca ggtggaaccg cttaccgaac
    12121 aggtgaaacg cttcacgctg gtggcaaccg atggcaaacc gctgcctgcg ttcaccggag
    12181 gaagtcacat cattgtgcag atgagcgatg gtgataacca gtacagcaat gcgtattcac
    12241 tactgagttc gccgcatgac acctcttgtt atcagattgc cgttcggctg gaggaaaact
    12301 cgcgcggcgg ttcccgcttt ttgcatcagc aggtaaaagt gggcgatcgg ttaacgattt
    12361 caacgcctaa taacctgttt gcgctaattc cctcagccag aaagcatctg tttatcgcgg
    12421 gcggtattgg tatcacccct ttcctgtcgc acatggcaga gttgcaacac agcgacgtcg
    12481 actggcagct acattactgc tcgcgaaatc cagaaagttg cgcatttcgt gatgagctag
    12541 tccagcatcc gcaggctgag aaagtccatt tgcatcattc gtcaaccgga acacgactgg
    12601 aattagcgcg attattggcg gatatcgaac ctggcacaca cgtttatacc tgtggccccg
    12661 aggcgctaaa tgaagcggta agaagtgaag ctgcgcgtct ggacatcgcc gccgatacgc
    12721 tgcactttga gcaatttgct atcgaagaca aaaccggcga tgcctttacc ctggtgcttg
    12781 cccgttccgg aaaagagttt gtggtgccgg aagagatgac tattttgcag gttattgaaa
    12841 ataataaagc cgcgaaagtg gaatgtttat gtcgtgaagg ggtatgcgga acctgcgaaa
    12901 cagcaatact ggaaggtgaa gctgaccatc gggatcagta ttttagcgat gaagagcgtg
    12961 ccagccagca aagtatgttg atctgttgtt cgcgtgcgaa gggtaaacgc ctggtgttgg
    13021 atttgtagtt tgccaaagcc ggatgtggcg ctgagcgcgc ccagtccggc ttcggaagat
    13081 tttactgcgg atattcctgc aataaattgt gtaatgcttc cgccatcagc tcaccacgcc
    13141 agccggaaat cagctccggc aaattgttct gcggtttcag tttccagtgc cagttcagca
    13201 gttggttgat ttgccgacgc gatgccagca attcggcgct gatcttatgc gtttcgctca
    13261 cgtcagtaat cagcgactta atcgctttaa acgctttacg ataacccggc atgtccatca
    13321 ggttaagcat cggctgcggt aaggcctctt ccggcaatgt ctgcgctttt tccaccagcg
    13381 ccagcagcgt tttaccgtga aagcggattt cgctaccgga taaacccagg ctgtccagtt
    13441 cgcctaaact tcccggcata taacgcgcga ccgaccacaa atgctcttca cgcacgacaa
    13501 agttcaccgc cagatcacgc tcacgagcct tgcgcagtcg ccagtcggct aacagttgca
    13561 gacaggccag ttggcgtgtg cgtaattgcc aggcattggt gatatcacgc caggcatctt
    13621 ccggcgcaac gacttcctga cgacgcattt gcatcaggcg gcattcatcc agcgccgcag
    13681 gtagccagcc ggaggcctcc gtttctacca taagcttggc ggtgatcggt aacagatacc
    13741 agacatccgc cgctgcgtat tcacactgac gttcggtcag cggtctggcc agccagtcgg
    13801 tgcgcgattc actcttgtcc agcgtaacgc cggaatactc ttccaccatg gaagcgaaac
    13861 cccatgacat cgggcgtccg cagaaggcag caaggatttg cgtgtcaatc aggggttgtg
    13921 gtaattcgcc aaagacattg aggaacactt ccagatcttc actgcctgca tggagaaatt
    13981 ttgtgatgga cggatcgcgc aggatcgctt tcagcggtga ccagtcggtg atcccgagag
    14041 gatcaattag cgccagatgc tcaccatcga aaagttgaat caaccccagc tgcgggtaat
    14101 aagtgcgcgt acgaacaaat tcagtatcct gggctatcgc cggaaagaca cggacggctt
    14161 cacacaaaga agccagcgca tcgtccgtgg taatcatttg gtaattcaaa ttgtattctc
    14221 tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc tgacgactga cttaacgctc
    14281 aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc aaaatttttc
    14341 cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact ttgtagcccg
    14401 tgagctgacg gcagcaaaag gtcaccagtg actcttcggt aagcgatgga tcttttttca
    14461 ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca gccgcgactt
    14521 cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag acgttaaaac
    14581 cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaaccct tcttcatcca
    14641 tcaccgcgat gtcgccggtg tgtaaccagc catttttgat gatctcatcg gtagcatcgg
    14701 gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca cccggttgac
    14761 ccggtggtac ttcattatcg tcgtcatcca ccagtttggc ttccgtcgac ggcaccggca
    14821 aaccgatgct accactatga taatcaatat catatgggtt aacgctgacc agcggcgcac
    14881 actcggtaag accatagcct tccagcagat actgtcctgt cagtttcacc caacgctctg
    14941 ccaccacttg ctgcactggc atcccaccgc ctgcggaaag atgcagactg gagaaatcca
    15001 gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg ccagtgatag
    15061 cggtaaacgg atatttcgct aactctttta ccaaccctgg aatatcgcgc gggttagtga
    15121 taagcaggtt ctgcccaccc agttcgataa acagcaggca gttaatggtc agggcaaaaa
    15181 tgtgatacag cggcagtgcc gttactacca gttctttacc tggatgcagc aacggcccat
    15241 aggtggcgtt gacctgttcc aggttcgcca gcatattgcg gtgagtcagc atcgcgcctt
    15301 tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc ggcaccagtt
    15361 cgggtttgac gtactgcatc cggtagccgt tatgcagtgc gctacgaaat gaaatggcat
    15421 ctggcagatg gtatttcggc accaaacgct tgatgtattt aacaacgaaa ttgactaccg
    15481 tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc tgaacggcgg
    15541 ttttatcaac cactttttcc agtgtgtgag caaagttaga cacgataaca atcgccgatg
    15601 cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg ttaacgttta
    15661 cgacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc aataaattag
    15721 gcatcatcaa cgcaacgcga tcgcctttct tcagccccaa cccttgttgc aaataagcgg
    15781 caaacgcgcg actgcgttct tccagcttgc ggaaggtcat tacctccccc atattcacaa
    15841 acgcaggctg atcggcgtag cggacgaccg actgctcaaa catatctatc agagattgat
    15901 aacggtcagg gttgatctcc gtcggaacat ccgcgggata acggttaagc caaaccttct
    15961 tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaagt gataaacatg
    16021 ttgttaacat aacattaact catcttacca gcttgataat tagacaacga aaaggttgcg
    16081 aagcgcgtca ctatttattt ttatagttgc cgtatgaacg cagaaacagc ggaccagccg
    16141 ctgtttcttt ttctttaaaa acaacttgtt actcggttac aattgtttca actcttgcag
    16201 ggccaggatt ataccaaccc catccgccgt aaccataggg ccagccgcgt ccaccataaa
    16261 accatggatc aattggctgc ggcggcataa tcacctgttg agtgatatgc cagcgtttgt
    16321 aacctgttac ctgcataacc ataaatttat atggtgcatt atttactttg ccatccactg
    16381 tgccagtgat tggcccgaca accgtcacca gttgtccacg aaaatcgacc ggatcaagaa
    16441 aaccgttcac atcggcataa atgcgcccgc gtgaaggttc tcccaaggtt ggtcttgctc
    16501 cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg ttttgtacca
    16561 caaccacttt gccgccaaag cgcgcctcct gaccaacata taaatctggc gcattcatca
    16621 cgcgtaccag atcttgctgt ggtgttggac tgctgccttt aatggcgtcc ggcacagtga
    16681 cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata acattctttt
    16741 gaaccgccat gatgcgactc ctttttttct cagggcatac tcttaagatt cattctttgc
    16801 ccggaagttt cttccatgcg acgttgttac gtaaataaac cggttcggca tgttccaccg
    16861 ccaccgtttt accctcggca aacatctgac acgcaatcgg cagcatatct tccgccgctg
    16921 gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc tttacggaga tccggccagg
    16981 cttgccagcc cgtccctacc gttacccatt cgccggaaag ctgttgcatt cgttcatgaa
    17041 cgagttcggg tttgagtacg gcttcggttt cttcaccgcg ccagataccg ttttcatcac
    17101 gctgatattc ggcccagtaa acttcgccca ttcgcgcgtc aatggctgcc agcacgcggg
    17161 ttgcgccgtt tttgcgccac gccccctgcg ccatcgtcat tagcgtagag acaccaatca
    17221 tcggtaactc cgcgccaagc gccagccctt gcgcaatacc aataccaatg cgcacgccag
    17281 taaagctacc ggggccgcgc ccgtaagcca gagcgtttat gtcagtcagg gaagttccgc
    17341 tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca cgagggcaaa
    17401 gctcaaaatg agcgttgaca ttaccgtcgt tccacagagc gacagagcag acctctgtcg
    17461 cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa aaggcgcgca
    17521 tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa cgaaccgcac
    17581 gggcaatgtc acgggtgcgt ggcgcgggcg gcagactggc gagaaacgtc gcgccgtaag
    17641 gacgcatcac cagccgattg tcacaaatta ccagcacgcc gcgatcgtcg gcgtcgcgaa
    17701 tcagtcgccc tactccctgc ttgagagtaa tgaccgcatc cggcagctgc acttcatcga
    17761 acgggtcgcc accgcgcaaa cgacaatctt ccatgcgcgc ttttaacagt agatcatccg
    17821 gcgaggtaaa cggcaatttg tcgataatta ccaatgacaa tgtatcgcca cgcacgtcca
    17881 ccccttccca gaaactactg gttgccacaa gaagcgcgtt accggcgctg acaaattgct
    17941 gcaacagttg ccctttgctg gtttccccct gcaacaaaac gggaagcgtc atggtagcgc
    18001 ggaactgctc ggccagatcg cgcatcatgg catgcgaggt acaaagcata aaacaacgac
    18061 cgttgttagc ttcgatgatc ggtcgcagca ttgccgccag ttgccgtgca caacccggtt
    18121 gattggtttg cggcaggttg cgcggcacac agagtaacgc ctggcggctg taatcaaacg
    18181 ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg aaatgatgca
    18241 gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccgggc ttttgcgcca
    18301 ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta aaatggcgcg
    18361 aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc cgcttcagcc
    18421 gtgtgcgata caacgtggcg cgctcaaatg ccgcatccag caaggcggaa cgccccagtg
    18481 acagtttcgc cacgtcataa caaagttcca gggtgtcatc gagcagtaaa aatgcccgct
    18541 gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctggc tcaccgagtt
    18601 gcagacgaaa atcctgtgca ctctgggcaa gacgatcagc gcacttttgt aactgctggg
    18661 tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg agcagttgtc
    18721 gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg tgggcttcgt
    18781 cgaagatcat gacgtccgct tcagggatca actcgccaaa tccactctct ttaaccacca
    18841 tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc gcttttttac
    18901 gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga cagttgtcgt
    18961 tggtgctggt gaccagcggc cacgcctgtg aatcttccgc cacgctgacg caggtgctga
    19021 tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg cttaagattt
    19081 gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg cagaggtagt
    19141 ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt gagactgttg
    19201 gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag ataatgactt
    19261 tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta ccggttcctg
    19321 cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc gccatctgtc
    19381 gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg tctggtgcaa
    19441 aatcgtccgt cacactaccc cctgttgatt tgaacaggga ttatgtcagg atgagggcgc
    19501 tttcgccagt tgaagtggtg acggcaacct cacattgtgg cagtctttgc agcacgaaaa
    19561 tggaagtata atgaggacaa aatgatgact atcgttcgta tcgatgctga agcccgctgg
    19621 tctgatgtag taatccacaa caacacgctc tactacactg gtgtaccgga aaacctcgac
    19681 gccgatgcct ttgagcaaac cgccaacacg ctggcacaga ttgacgccgt gctggaaaaa
    19741 cagggcagca ataaatcgag cattctggat gccaccattt tcctggccga taaaaacgac
    19801 ttcgcggcga tgaataaagc gtgggatgcc tgggttgtcg cgggtcatgc gccagtgcgc
    19861 tgcacggtac aagcgggttt gatgaacccg aagtataaag ttgaaattaa gattgtggct
    19921 gcggtgtaag ctttatcgaa gcaaaataag tcagacgata atttatcgat aatactggtc
    19981 ggttttacat aaatcgacca gagaataaga tattactgtt gatgcttctg ctaattcatt
    20041 atttatattt ataatttcaa ttttatctat aaaactaact atataaataa acataaccat
    20101 gccgttgtgt tcgccctttg attagcaact ctggttttct ttcttagctg aacaatctgg
    20161 ttatagcgtg cggactgacc gttaattttc tccttcttac tggataacac accatgagct
    20221 gctaccttta aaacacctga atgtcaacag gttaactcgt gccatatcgt tataatccct
    20281 ttgatattag atgcaaatt
//
LOCUS       DAZPJR010000066        20147 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPJR010000066 DAZPJR010000000
VERSION     DAZPJR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.067"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="ACHVB7_002241"
     CDS             complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="ACHVB7_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR1914217.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
     gene            complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="ACHVB7_002242"
     CDS             complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="ACHVB7_002242"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR1914218.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            2790..3560
                     /gene="yqeC"
                     /locus_tag="ACHVB7_002243"
     CDS             2790..3560
                     /gene="yqeC"
                     /locus_tag="ACHVB7_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR1914219.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            3608..5233
                     /gene="yqeB"
                     /locus_tag="ACHVB7_002244"
     CDS             3608..5233
                     /gene="yqeB"
                     /locus_tag="ACHVB7_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR1914220.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="ACHVB7_002245"
     CDS             complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="ACHVB7_002245"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1914221.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFVRSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="ACHVB7_002246"
                     /pseudo
     CDS             complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="ACHVB7_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            complement(7691..8902)
                     /locus_tag="ACHVB7_002247"
     CDS             complement(7691..8902)
                     /locus_tag="ACHVB7_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR1914222.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="ACHVB7_002248"
     CDS             complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="ACHVB7_002248"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR1914223.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="ACHVB7_002249"
     CDS             complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="ACHVB7_002249"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR1914224.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            11880..13658
                     /locus_tag="ACHVB7_002250"
     CDS             11880..13658
                     /locus_tag="ACHVB7_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR1914225.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="ACHVB7_002251"
     CDS             complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="ACHVB7_002251"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR1914226.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="ACHVB7_002252"
     CDS             complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="ACHVB7_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR1914227.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="ACHVB7_002253"
     CDS             complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="ACHVB7_002253"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR1914228.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            17775..18530
                     /gene="actS"
                     /locus_tag="ACHVB7_002254"
     CDS             17775..18530
                     /gene="actS"
                     /locus_tag="ACHVB7_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR1914229.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            18609..18678
                     /locus_tag="ACHVB7_002255"
     tRNA            18609..18678
                     /locus_tag="ACHVB7_002255"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18641..18643,aa:Gly,seq:ccc)
     gene            18883..>20147
                     /locus_tag="ACHVB7_002256"
     CDS             18883..>20147
                     /locus_tag="ACHVB7_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR1914230.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT         4980 a         4765 c         5179 g         5223 t
ORIGIN      
        1 aactgaggaa tgatattttt caccacgccg ccagcattgg cttcgcgttc aaacagcgta
       61 accggatggc ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca
      121 atcacggcaa ccggatggcg tgaaccagaa ccggcgggtt tgtgccagcg ttgcttatat
      181 tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg
      241 taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggca
      301 ggtagggcgt tgcgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc
      361 agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg
      421 tagcaatcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt
      481 ttctgggtgt attccatggt taacgcgtct gctgccagtc tgttcagtcg ttcgacgtca
      541 acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc
      601 agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat acctgtatca
      661 aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg
      721 tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct
      781 gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta
      841 gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtaccatcag gcgctccagc
      901 atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg
      961 taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggtcggg
     1021 ttaagtttga caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg
     1081 atttcatgcg gaggacagcc gtgcatggtg gagaggggga cgccctgcac catactggtg
     1141 gggatgcgag cgggtaaggc ttgcaagttt tcgcgttttt cctgcaatcc gtggcgggct
     1201 aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc gaatttcgga
     1261 tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct
     1321 tcgaggttgt aaccgatgct catattaaag atgaacgatt taccggaatc agaaggctgt
     1381 aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta
     1441 agcaaggtaa attcggtaga ccattcggtg ttaaagcatt cgtcttcggc atcaatacag
     1501 ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg
     1561 ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg
     1621 ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag
     1681 taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg
     1741 cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta
     1801 gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga
     1861 gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggtggct
     1921 cgaaacttaa aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact
     1981 cctgaaaatt attattgtca gcccatttat cactttttct cttttctcaa aatcagtgat
     2041 tccttatcat tatgataaag agtgctggag agattaaata tttgaacgca atcgtttaac
     2101 tacattttct aaattttagt ataccttttt tgcgctgtaa taaagtcgtc cggagtatca
     2161 atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc cataaggagt
     2221 gcctgacgca tattggtgac attgggtcgc tggattgcct gcatcaggca tggttttgac
     2281 actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc atcatttcgt
     2341 aacgaccaga tttttctaaa aatatcgatg gtgagggttg gcatgtcacc gtgggtgaga
     2401 aaacaatgtt ctgtttgcac tgcgggtact gcggccttga cggatgttag taaaccctgc
     2461 gcataatctg ggttgtgaat aatagtgata ttgctctggt tcgcataacg ttcgtgcagt
     2521 tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa cgcatttttg
     2581 atacttgtat caagaattgt tccctgttcc caggggaaca tcattttcca ttgccccatt
     2641 cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt ggttccatta
     2701 tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta agttggggat
     2761 aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca tcggcattag
     2821 tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt gctgggggga
     2881 aaaccagctt gcttttttgg ctggcagaac tgctccaggc tagcggcagg cgtgtgttaa
     2941 tcacgacgac gacacatatg tttatgccaa cgtctcactg gcccgtggtt ttctgtcgtg
     3001 atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt tttcacagct
     3061 ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat gcactggtgc
     3121 aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga atgccgttaa
     3181 aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga
     3241 tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt
     3301 ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat ctcgttgcgc
     3361 tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt
     3421 tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc
     3481 tgcaacaaca cgacgtagag gcaatctggc tgggcgatat acaagaacat cctgcaatcg
     3541 cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga tatccattga
     3601 ggttggtatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc
     3661 gatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac aaatgttagt
     3721 gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc ggaaggtgat
     3781 tgaagagtca cttcaggcat tgcaggaacg taagccgcga ttattccatg gacgtatggc
     3841 tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag
     3901 cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca accgggcgat
     3961 agcccagagt gcggcgctat taggatttga tattgccgtt gccgatattt atcgcgaaag
     4021 cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt cgtttggtgc
     4081 ggcagtggaa gaactggata ttcgccctga taattttgtc ctgattgcca cgaataatca
     4141 ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg ggttgctggc
     4201 aagtcgccgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga
     4261 acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga
     4321 gatcgccatc agcgtgctgg cagaaatatt atgggtgaaa aataacgcgc cgggtgggct
     4381 gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg gtgcgggaga
     4441 tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga tcatgctgga
     4501 agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg
     4561 cgaaatgacg gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg aagcgatgaa
     4621 acttaccgaa cgcggattca tccctgtgat ggtagatccc gcctgttcat tgcttgatga
     4681 actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg gaacgcgggc
     4741 agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgcaggga aggattgtca
     4801 tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact ctggttgtgc
     4861 gcaggagaat accgatgttc ctggcaatat tatggggcat accacccgac gggtgatccg
     4921 tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg
     4981 cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg ggatggtgcg
     5041 tggcttgttg aacgacggcc tggcagtggt tggtggtttt aaaattggtg atatcgatcc
     5101 tcgtggtgaa accgctgatt tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg
     5161 cgtacttgag gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct
     5221 ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctta aaattactga
     5281 caaatcaccg ttccggtttt gccttcaatc ccttctttcg ctttgctcaa tacggtaatc
     5341 agtgcttcac gacctgcgcg agagcgaaca aacgaggcgg cagcttccac tttaggcagc
     5401 atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcact taatgacagg
     5461 cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc taccgcagtc
     5521 agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc ccagtcttta
     5581 tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg gataccacca
     5641 ccgccaacgg cgatcaccac ctggcctgca tctaccagag ctttaaccgt ttctttttca
     5701 atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc agcatcttct
     5761 ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg
     5821 atcggcttgg tcgggttgag gaatgccgga tcgttagcgt cgacttcgac ctgagtaacc
     5881 agcgttgcta caggtttatt aatgccccga gaaagcagtt cttctcttaa cgcgttttgt
     5941 agatcgtaac caatataacc ctggcttaag gcaacacaga cagacatcgg cagcattggc
     6001 gagtgtgctt cagtttttgc ggcagcttca aaggcttggt taatcatgcc cacctgcggc
     6061 ccgttaccat gagtgacgat aacttcatgt ccttgggcaa ttaaatcaac aattgcctga
     6121 gaagtaattt ttaccgcttt catttgtccg gccaggtcat cgcccagcgc attcccgccc
     6181 agggcgagaa caattttctt actcattttt cctcactcat cattcttgat tgtgtataga
     6241 tccctgacag tggttagagc acgggaggga caaacggttt gcgtcgcagg aaacgacctc
     6301 gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc
     6361 tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga
     6421 gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg tcgccatcgg
     6481 aaccgggcgc taataatcct ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc
     6541 tggttaattc aacaaagcgt tccggtgtta tacgtcccgt catcacgcca ctggaaaaca
     6601 gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg aaatcgcctt
     6661 tagaaatttg caggcgttga gccatcgaga aggtgcagtg atcggtcgcc accacgtcaa
     6721 tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg
     6781 ggctaagaat gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc aacaggagat
     6841 attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga cgcagataat
     6901 ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg
     6961 ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga ctcaaggcat
     7021 gatagcgcgg cgcggttaac cctgcggcga taaattccgc ccgcttgctg gcgatagccg
     7081 catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcaagcgg cgtaatgcct
     7141 gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta aaactgctca
     7201 gtccttcctc gaccatcatc gggatttcgt cgaggattgc gtgattaatg tgctggatca
     7261 caccgtgaaa gctgtaatcg atgaccgctt tatgggcggc ataaccacga taaacctcca
     7321 gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata atggttgttg
     7381 taccgccaca cgcagctgcg cgggtaccgg taaaaaatca tcacaactgc gcgcgatgcc
     7441 gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt aacagccagt
     7501 ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact ggcgaacaat
     7561 cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga cagtgccgtt
     7621 tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga ggggaataag
     7681 tgaagagtaa ttactctgtt gccagccagc ttaacgggat tgcagcgtac atcgcggcac
     7741 aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca ggttctttac
     7801 ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg ccgttagttg
     7861 agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag gcattcacca
     7921 gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag cattcggttg
     7981 ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca accgcgttag
     8041 ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag gtttcgcccc
     8101 aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa cggcttgggg
     8161 atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg tcataaccca
     8221 gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa atggcgttgt
     8281 caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata cgaatttcca
     8341 tacgaccgcg ctgaccacgg tatatctggc agtcggttgg ttcggtactg accacaaatt
     8401 ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg cagtcttctt
     8461 cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg tctttaatga
     8521 ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta ccgcgaccac
     8581 cgattagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt ttgatgttgc
     8641 caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg tggccgatat
     8701 aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact ttttccatct
     8761 cttctttaat acgatgtact acgcgttttt cgtcgcagct ttcactggga atagcaacca
     8821 tgtcgcgcag gaagcgagtc atgtccgcct ggtaatcttt tgctttttca agaatcagtt
     8881 tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg ttttgcaggg
     8941 agtagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag acaacttcgc
     9001 ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcactacg gcatctttgt
     9061 ccagcgccag tttttccatc aggctttgat gttgcgggtg ataatgaacc gctgcgagaa
     9121 cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg ccgtacggat
     9181 tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg aattgggtgg
     9241 cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc aggcccgcca
     9301 tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg gagcgataaa
     9361 tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg ctgtagacgt
     9421 cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc agcagaacgt
     9481 gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc agggttgcgt
     9541 agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg tcctgtacca
     9601 cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg tcatagttca
     9661 tatccgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt tcctgagcag
     9721 aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc cacgccacac
     9781 cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg atggcatttt
     9841 tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac aataattgag
     9901 cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt tttgattcgt
     9961 ctttgacgag aattttcttc acaccaaaaa ggtttgcgag atcgtccagc gcacaaagcg
    10021 gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg gcctggcttt
    10081 gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata tcaatcttca
    10141 atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc gaccagcaga
    10201 tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc tcgagcagtg
    10261 cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa ggcttccagc
    10321 tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc acttcacctt
    10381 ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg gcttcgccat
    10441 cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc gcgttctgtg
    10501 ccagacactg tttttccagt gcttttaagc cttcgtggtc gttcgcacgc agcaattcag
    10561 tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg tctgcgtctt
    10621 tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct ttagcattgt
    10681 ttttcgccac ttcaaccaca tccgggatca ggtcgtagcc ttccggatgg gccaaggtga
    10741 catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag agcggtttgc
    10801 catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg ttttccagtg
    10861 aaccaaagtg ttcacgtaac cacgcgaggt cagccattga ctgagtcggg tggtcaatat
    10921 cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg taaccgtcat
    10981 caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac atatcgtcgc
    11041 gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc acggtttcgc
    11101 cgtgagcgat ttgtgatttg ccttcgtcga gatcttgttg tgcaaggccg agcaggttaa
    11161 gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg gaaataccta
    11221 atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt aatgctgcgg
    11281 caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac aaaaaatctt
    11341 tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta acagttttca
    11401 tcatacagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt gacttgccaa
    11461 agtgattggt gttttcattg ctatacctgg gactaacaat aagtgtgcca gaattatccg
    11521 cgcggctgca aaagactaat ttgacggata atgtgtgggg ttgttcacaa attgatgcca
    11581 gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt tatttatttt
    11641 ctgctaagta tcagaaataa gataaagggg caggctatct ctcttcagtt tgataaactc
    11701 accgccgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat tgcattagaa
    11761 aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat atcatcatga
    11821 tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa ggtaaagcta
    11881 tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt cagcgttttg
    11941 ccagaatgct tgccagcgtt ttgcagtttg aggttgagat cgttgatgaa aacttgtgtc
    12001 gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc ggcaactcac
    12061 gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag tctcgcttcg
    12121 atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca tttctgggta
    12181 cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc gttacccacg
    12241 agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt cgccatatat
    12301 ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac atcagtaaaa
    12361 tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat gatgaaagtc
    12421 gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa aataatatta
    12481 ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact catggacata
    12541 tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa ttgcataaca
    12601 ttcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt ttttctgtag
    12661 caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt gttatgcggc
    12721 aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg gtggttggtg
    12781 aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc ggaagacgga
    12841 ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg gaaagcgaac
    12901 tgttcggtta tgttaaaggc gcatttactg gtgcttctgc caacggtaaa acagggttga
    12961 ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg ccattaatgt
    13021 tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt ggtgccagta
    13081 gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg gcccagttca
    13141 ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc ccgataactc
    13201 tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac tttttacatc
    13261 tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc gtcgaaatat
    13321 tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg gaatatctgg
    13381 ttaacgtggt tccttcaggt gaagttatcg acagcacgct attgccgcca aatctgctga
    13441 ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac ctgtcactcg
    13501 atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc gaggcgcttt
    13561 cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt gctacgctct
    13621 atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc cggcctgaat
    13681 tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt tacaatcatc
    13741 tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc cgtaatggtt
    13801 aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag gccaggcgta
    13861 caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg agaaagtttt
    13921 ccgcctttcg cttcaccttc cagcgtgcgg atctcctttc cttcagccca ggcggcaagg
    13981 tataagcaac tgtctattgc tgtgccgtcg accaacaccg tacaggcacc acattcaccc
    14041 acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc cgagagcggc
    14101 gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc gatggtaatt
    14161 gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg ctaatcactt
    14221 ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc catgaagaac
    14281 gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc aggtttaatg
    14341 gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac
    14401 caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc
    14461 caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt
    14521 gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc tcaagagaca
    14581 ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg cgcggggagt
    14641 ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg ctggtggcac
    14701 cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca gcaatggacg
    14761 tggccgcagc acataacgcc gggagatgac gttgagttat aggagcttct attagctggg
    14821 taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta attccccgca
    14881 gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga tggtggagct
    14941 gaatcagtac gtcagtgcca tcggcgagca gtttggcctg cgggttgtca gccagcaggt
    15001 tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat
    15061 cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt cagcggcagt
    15121 gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc ggcaacagga
    15181 attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc attgatttcg
    15241 acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta atccagcaga
    15301 ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa tagcgcccag
    15361 ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg attaagaata
    15421 tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag cgcaatatcg
    15481 accgtcagat caacaaaggt acagccaaac gccgatgggt tagtggtggt tttgatggag
    15541 ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat cgccaaatct
    15601 tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc ttttatcagg
    15661 gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat tttctctttt
    15721 aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg tgaggcaaat
    15781 gcgccgggag cgaacggcgt aacgtcggta tcttgagttg aaataacgcg aacgtcgctg
    15841 accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc accctgaccg
    15901 atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag aaggcgcgcg
    15961 cctgctattt ctacgccgac aggccaggtg ttagaggtat agctaaaaca ggcgacgcca
    16021 acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt ttcccattca
    16081 aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat acgtttgccc
    16141 gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca
    16201 atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac gacttgtggc
    16261 gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt ctttgaactg
    16321 taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcctccccgc agaagcgatg
    16381 gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact ataacctttc
    16441 aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg gcgggtacgg
    16501 gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc gccaagcttg
    16561 ctggtcagga atgctgccat tggctcttcc agtacatcct gtttattacc aaaaccgcca
    16621 ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc
    16681 acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg cgagtcatcc
    16741 tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac gggggtctga
    16801 tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt acccgtcgac
    16861 atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc ttctgccagc
    16921 gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac caattgcgcc
    16981 gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc accatgatga
    17041 cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc
    17101 caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaggt aaaaatcgcc
    17161 agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata accatgtgcg
    17221 atagggctac gtacatattt cgcataacac atgcccgcca taacataatc gtcagtatat
    17281 cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc acccgtagcg
    17341 gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc ttatggcaaa
    17401 gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta ggggaggtag
    17461 gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct gaataggggc
    17521 agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattggg attattacgc
    17581 atcaggatgg gaaatagggt aaaaagtcag gaagttcgat acctctacag cgaatatttt
    17641 gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac cacgtttttc
    17701 cagaagcaag agattgggta tcaagaggct ggctgctatg ataaggcgtc ttgtttttta
    17761 agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt atcgtgatgt
    17821 tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc gatacaggaa
    17881 cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt atttcgcgca
    17941 ccacgggaac cagcgtaaaa gaactggcgc gactgaacgg catttccccc ccttacacca
    18001 ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt acacgtaaat
    18061 caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg gtaccgaaat
    18121 catcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg aaagttatca
    18181 tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct ccacggggta
    18241 cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct
    18301 acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac gcccataatg
    18361 acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg
    18421 ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt gcaacggcaa
    18481 ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga tggcgaatta
    18541 atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa tgccttacgc
    18601 atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct atacgagggt
    18661 tcgattccct tcgccgcttt tgctgtatct gattttctat attatcttaa ttcaatcgct
    18721 tcagactttt cgcaatcgcc gtaatatttc cccattttgt tgtaaataaa aagcgtttac
    18781 gcaatgcaat ccttttgtaa caacccaggt aaaacacctt attttcaagt tgttaaataa
    18841 gtggttattt acgcttttaa caaacgattg atggaatatt ttatgaaagg gaaatctgca
    18901 ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac aggggcagaa
    18961 gtgacgagtg aatctgtatt taacattctt aattccacgg gggcagcaac agataaaagt
    19021 tatttatcgt taaatccaga taagtaccct aattatcgtc ttctgattca ctcagccaaa
    19081 ttaaaaaatg aaataaagag ccactatacc aaagacgaaa ttcaagggtt gttaacatta
    19141 acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacatt tattcttgct
    19201 tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt ctggctgagg
    19261 gatagtttat ggggctatat ggcattagta tcagatcagg gaaatagtgt agccgcaaaa
    19321 aaggttctgc tcaccctgtg ggattatatg tctacgctcg accagattaa gcggatgcag
    19381 gacgtcatta gcaaccccaa acgactggat ggagttcccg ggcaaatgaa tgctgtgcat
    19441 atccgatttg acagcaactc ccccgtgatg gcagatgtgc aggaagaagg caagccgcag
    19501 ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc ttgatctcct tatacaagca
    19561 atcgacactg gtacaattaa cgctgaagat tggcaaaaag gtgaccgatt aaaatcggtt
    19621 gcgctgctga ttgcttatct ggataaagcc aatttctatg ttatggaaga ttccggagca
    19681 tgggaagaag atgcccgatt aaacacatct tccgttgcac tggtgacctc tggactggaa
    19741 cgtttgtcga acttactctc gaagaaagac tctgtttttg tctctgattt actacgcgaa
    19801 gctaaagcca atgaactgga tgaacctctt tcaacaacga ggttgaacca cctcatagat
    19861 aaaggttatg aaagaataac gttacagctt gatttagggg gagaatcccc aggttatctg
    19921 gaaaaagata aacattacag agaagccgat gcggctttac ttaatgttat ttatcctgca
    19981 aatcttgcaa agataaacac cagaagaaaa gagcaggttc taaaaatagt taaaaaacta
    20041 gcaggtccgt atggtataaa aagatacgag aaggataatt atcagtcggc aaatttttgg
    20101 ttcaatgata ttaaaaccga taccgatcaa aattcacatg cgaaaag
//
LOCUS       DAZPJR010000067        20039 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPJR010000067 DAZPJR010000000
VERSION     DAZPJR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20039
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.068"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            77..211
                     /locus_tag="ACHVB7_002257"
     CDS             77..211
                     /locus_tag="ACHVB7_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1914231.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            190..294
                     /gene="speFL"
                     /locus_tag="ACHVB7_002258"
     CDS             190..294
                     /gene="speFL"
                     /locus_tag="ACHVB7_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR1914232.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            605..2803
                     /gene="speF"
                     /locus_tag="ACHVB7_002259"
     CDS             605..2803
                     /gene="speF"
                     /locus_tag="ACHVB7_002259"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR1914233.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2800..4119
                     /gene="potE"
                     /locus_tag="ACHVB7_002260"
     CDS             2800..4119
                     /gene="potE"
                     /locus_tag="ACHVB7_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR1914234.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4175..5815)
                     /gene="pgm"
                     /locus_tag="ACHVB7_002261"
     CDS             complement(4175..5815)
                     /gene="pgm"
                     /locus_tag="ACHVB7_002261"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR1914235.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5841..6386)
                     /gene="seqA"
                     /locus_tag="ACHVB7_002262"
     CDS             complement(5841..6386)
                     /gene="seqA"
                     /locus_tag="ACHVB7_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR1914236.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6571..7335
                     /gene="ybfF"
                     /locus_tag="ACHVB7_002263"
     CDS             6571..7335
                     /gene="ybfF"
                     /locus_tag="ACHVB7_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1914237.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7475..7768
                     /gene="ybfE"
                     /locus_tag="ACHVB7_002264"
     CDS             7475..7768
                     /gene="ybfE"
                     /locus_tag="ACHVB7_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR1914238.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7908..8438
                     /gene="fldA"
                     /locus_tag="ACHVB7_002265"
     CDS             7908..8438
                     /gene="fldA"
                     /locus_tag="ACHVB7_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR1914239.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8648..8734
                     /gene="uof"
                     /locus_tag="ACHVB7_002266"
     CDS             8648..8734
                     /gene="uof"
                     /locus_tag="ACHVB7_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR1914240.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8727..9173
                     /gene="fur"
                     /locus_tag="ACHVB7_002267"
     CDS             8727..9173
                     /gene="fur"
                     /locus_tag="ACHVB7_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR1914241.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9258..9584)
                     /gene="chiQ"
                     /locus_tag="ACHVB7_002268"
     CDS             complement(9258..9584)
                     /gene="chiQ"
                     /locus_tag="ACHVB7_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR1914242.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9634..11040)
                     /gene="chiP"
                     /locus_tag="ACHVB7_002269"
                     /pseudo
     CDS             complement(9634..11040)
                     /gene="chiP"
                     /locus_tag="ACHVB7_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(11618..13282)
                     /gene="glnS"
                     /locus_tag="ACHVB7_002270"
     CDS             complement(11618..13282)
                     /gene="glnS"
                     /locus_tag="ACHVB7_002270"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR1914243.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13542..15488)
                     /gene="nagE"
                     /locus_tag="ACHVB7_002271"
     CDS             complement(13542..15488)
                     /gene="nagE"
                     /locus_tag="ACHVB7_002271"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR1914244.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            15821..16621
                     /gene="nagB"
                     /locus_tag="ACHVB7_002272"
     CDS             15821..16621
                     /gene="nagB"
                     /locus_tag="ACHVB7_002272"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1914245.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16681..17829
                     /gene="nagA"
                     /locus_tag="ACHVB7_002273"
     CDS             16681..17829
                     /gene="nagA"
                     /locus_tag="ACHVB7_002273"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1914246.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            17838..19058
                     /gene="nagC"
                     /locus_tag="ACHVB7_002274"
                     /gene_synonym="nagR"
     CDS             17838..19058
                     /gene="nagC"
                     /locus_tag="ACHVB7_002274"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR1914247.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            19106..19858
                     /gene="nagD"
                     /locus_tag="ACHVB7_002275"
     CDS             19106..19858
                     /gene="nagD"
                     /locus_tag="ACHVB7_002275"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR1914248.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(19909..20039)
                     /locus_tag="ACHVB7_002276"
                     /pseudo
     CDS             complement(19909..20039)
                     /locus_tag="ACHVB7_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4911 a         5064 c         5032 g         5032 t
ORIGIN      
        1 ttaacgcaaa tataaaacac gaaattacaa catttaagta acaaaacagc atctaatatg
       61 gcgatattca taattaatga tttattatct ggggtatgat cgttttttgt tgatcttctt
      121 cacagattat agccatttca tggatagaat aactctacct tcaactgaca cagcaagagg
      181 taaaggtaaa tggaaaataa taaccgctta atgcctcata taaggcggac aacccatatc
      241 atgaagtttg cccatcgtaa tagttttgac tttcatttct ttaatgcccg ctagtcttct
      301 gactaaaggg caccccaacg tacaggtctc cctgacttta agcattacag gttaatacct
      361 gtattcctcg gtgctcatat actgctaacc cttttaaact ctaaataatt cgagtcgcag
      421 cacttgcaac ttgaggtatg acgagtatag ccagttaccg ggctggtctg ggttattgca
      481 tctgcaaaaa gcaaactact gatttattta tcagcggtgg agctttgctt tttttccggc
      541 gtgatcgatt tctcctttga gaaattgagg acctgctatt acctgaaata aagagatgaa
      601 taaaatgtca gaattaaaaa ttgccgttag ccgttcttgc ccggattgtt tttccactca
      661 tcgtgcatgc gtgaatattg acgaaagtaa ttatattgac gttgccgcca ttattttatc
      721 agtcaatgat gttgaacgtg gaaaactcga tgaaatagac gctactggct atggcattcc
      781 tgtttttatt gcaacggaaa atgaagaacg tgtaccagca gaatatctgc cacgtatttc
      841 tggtgtcttc gagcattgcg aatcacggaa agagttttat ggacgtcagt tagaaaccgc
      901 tgctagccat tatgaaacgc aactgcgccc gcccttcttc cgcgcactgg tcgattatgt
      961 caatcagggc aatagcgctt ttgactgccc gggtcatcag ggaggtgagt ttttccgtcg
     1021 tcatccggca gggaatcagt tcgtggagta cttcggagaa acgctgttcc gttccgactt
     1081 atgcaacgca gacgtagcaa tgggcgatct gctgattcac gaaggcgcgc catgcattgc
     1141 acagcaacat gcggcaaaag tgtttaatgc cgataaaaca tacttcgttc taaatggcac
     1201 ttcatcttct aacaaagtgg ttttaaacgc cctgctaaca ccgggtgatc tggtgctgtt
     1261 tgatcgcaat aaccacaaat ctaaccacca cggagcgttg ctacaggctg gtgcaacacc
     1321 ggtttatctg gaaacggcac gtaacccgta tggctttatc ggtggcattg atgcgcactg
     1381 ttttgaagaa agttacctgc gtgagctgat cgcggaagtc gcaccgcagc gggcaaaaga
     1441 ggctcgtcct ttccgcctcg ctgtgattca gttaggcacc tacgacggta cgatttataa
     1501 cgcccgccag gtggtggata aaattggtca tctgtgtgac tacattctgt ttgactccgc
     1561 atgggttggc tatgagcaat ttatcccgat gatggctgac tgttcgccgc tgttgctgga
     1621 tcttaatgag aacgatccgg gtattctggt tacgcaatcc gtacataaac agcaggctgg
     1681 tttttctcaa acttcacaaa tccataaaaa agacagccac atcaaagggc aacagcgtta
     1741 tgtaccgcac aaacgcatga acaacgcctt tatgatgcac gcctccacca gcccgttcta
     1801 tccgctgttt gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa
     1861 tatgtggatg gattgtgtgg taaatggcat taatgcccgc aaactgatcc tcgataactg
     1921 tcagcatatt cgtccgttcg tacctgaact ggtggatggt aaaccctggc agtcgtatga
     1981 aacagcgcaa attgcggttg atctgcgctt cttccagttt gtaccagggg aacactggca
     2041 ttcttttgaa ggctatgcag agaatcaata ctttgtcgat ccttgcaaac tgttgctgac
     2101 aaccccaggt attgatgcac gtaacggcga atatgaagcg ttcggtgtac ccgcgacgat
     2161 tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc ttaactccat
     2221 cctcttcctg ctgactccgg cagaagatat ggccaaactt cagcaacttg ttgccctgct
     2281 ggtacgcttc gaaaaactgc ttgagtccga cgcgccatta gcagaagtgc tgccttccat
     2341 ctacaaacag catgaagagc gctacgccgg ttataccctg cgtcagttgt gccaggaaat
     2401 gcatgatttg tatgcccgcc acaacgtgaa acaactgcaa aaagagatgt tccgcaaagc
     2461 gcacttccca cacgtcagca tgaatccgca agaagccaac tacgcctatt tacgcggtga
     2521 agtggagctg gttcgtctgc cggatgccga aggtcgtatc gctgccgaag gggcgcttcc
     2581 ttatcctccg ggtgtgctgt gtgttgttcc gggtgaaatc tggggtggtg ctgttttgcg
     2641 ttacttcagc gctctggaag aagggatcaa cctgctgcca ggttttgcac cggagctgca
     2701 gggtgtctat atcgaagaac atgatggtcg taagcaagtt tggtgctatg tcatcaagcc
     2761 tcgtgatgcg caaagcaccc tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaac
     2821 aaaatgggcg tcgttcagtt aaccatactg acgatggtca acatgatggg ctccggtatc
     2881 atcatgctgc cgacaaagct tgccgaagtt gggacaattt caattatctc ctggctggtg
     2941 acagccgtag gctcaatggc actggcatgg gcattcgcaa aatgcggtat gttcagccgt
     3001 aaatcaggtg gtatgggcgg ttatgccgag tatgctttcg gtaaatccgg taactttatg
     3061 gcgaactata cctacggcgt ctcactgctg attgctaacg tcgcgattgc tatttcggcg
     3121 gttggttacg gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg
     3181 accatcgggg tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg
     3241 caaatcagta gcattaccgt gtggggggtc attattccgg tggtaggcct gtgcattatc
     3301 ggctggttct ggtttagccc gacgctttac gttgattcct ggaatccgca tcatgcaccg
     3361 ttcttcagtg cagtaggttc ttccatcgct atgacgctgt gggcttttct cggtctggag
     3421 tctgcgtgtg cgaatactga tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta
     3481 ctcggcggta cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt gattgcaggg
     3541 attgtgccta atatggagct ggcaaattca acggcaccat ttggcctggc cttcgcacag
     3601 atgttcacac cagaagtggg taaagtcatt atggcgctga tggtgatgtc ctgctgcggt
     3661 tcgctgcttg gctggcagtt caccattgcc caggtgttta agtcttcatc tgatgaaggc
     3721 tacttcccta aaattttctc ccgtgtaacc aaagtggatg caccggtgca gggaatgttg
     3781 accattgtga ttatccagag cgggttggcg ctgatgacga ttagcccgtc gctaaacagt
     3841 cagttcaacg tgctggttaa cctggccgtg gtgaccaata tcattccgta tattctgtca
     3901 atggcggcat tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt
     3961 gcaaactttg ttgcttttgt tggcgcgatg tatagctttt atgcgctgta ctcatccggg
     4021 gaagaagcca tgctgtacgg ttccatcgtg accttccttg gttggacact gtatggtctg
     4081 gtatcaccac gctttgaact gaaaaataaa cacggttaaa aaagcaaagt aaaaaagggc
     4141 gatcttgcga ccgccctttt tttattaaat gtgtttacgc gtttttcaga acttcgctaa
     4201 caatctcaac cgcttctttc tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc
     4261 agtagatctt atatgcgtct tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag
     4321 tcatcacttt cagaccgccg atagaagcac cattgcccgg tgccgccgtc aggcgcgcgg
     4381 tgatcgggtc gcctgccagg gtgctggcgc tcaccatttc cggagacagc ttagacagcg
     4441 ccgctttttg tgcggaagtc gcagctgcct gcaaacggtt gtagctcggc gcaccaaagc
     4501 gttttgccag ttcgttgtag tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg
     4561 ccgccagcag acacatgatg atgccgtctt tgtcggtgga ccacggcgtg ccgtcgaaac
     4621 gcaggaagga agcccctgcg ctctcttcgc cgccaaagcc gaagctgccg tcgaacagac
     4681 catcaacaaa ccatttgaaa cctaccggga cttctaccag cttgcggccc aagtcattga
     4741 ccacacggtc gatcatcgca gaagaaacca gcgttttacc gacagcaaca tctttgcccc
     4801 actgcggacg atgctggaac aggtagttga tcgccaccgc caggtagtgg ttcggattca
     4861 tcaaacctgc cggagtgacg ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca
     4921 gatcgaactt atcacgcaat gccagcaggc ccgccatcgc acactcggag gagcagtcca
     4981 tacggatcgc gccgtcttta tcgaggtgca taaagcggaa ggtttgatcg acctgatcgt
     5041 taacgatggt caggttgagg ttgtaatact cgccaattcg cttccagtat tcgataccgg
     5101 aaccacccag cggatcaacg cccagcgtca ggcccgtttt ctgaatcgcc gccatatcaa
     5161 cgatatcggc cagaccttcc acaaactgct gcaccagatc ctgctctttc acatgaccgg
     5221 atgccatcgc ttcgtcgagg gagatacgct tcacgccttt caggccatcg gccatcagtg
     5281 cgttggcacg atcttccacc actttagtga cgttggtatc agccgggcca ccatttggcg
     5341 gattgtactt gataccacca tcttccggcg ggttatggga gggtgtaatc acgataccgt
     5401 ctgccagcgg gccacctttt ttattatgaa ccaggatggc attggaaacg gcaggcgtcg
     5461 gagtgaagcc attgttttcc tgcacaatga catcaacgcc gttcgctgcc agcacttcca
     5521 gtacggaaat gaacgcaggc tcggacaggg cgtgagtatc tttacccaca tagcaagggc
     5581 cagtgatgcc gtttttcgca cgttcttcag caattgcctg agcgatcgcc agaatgtgcg
     5641 gctcgttaaa gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg aatttcaccg
     5701 cgtgctccgc attccctgct tctggtttca gtacataata ttgcgccgtc agttgggcga
     5761 cgttaatcaa atcactctgt tgtgcaggtt ggcctgcacg attgtggatt gccattgctt
     5821 tgtcctttgt ctgcaacgtt ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg
     5881 aattgcatcg actgcatgat gtgttcgatc atgctgcatt tacggccggt gttggtgttg
     5941 gtgatcaccc aatacggcgt gcctggcaca tgtttcggct tggtctgatt accatttttc
     6001 agcagcgttt gttcatctgc cgcaaagtaa acgcgtgtac gaccgtgcaa cgattccgtt
     6061 gcttcggcaa acgcctgggc gtcaagagaa tatagtgtag acaacagcag cataaagcga
     6121 ttgaccgctc gcttttgctc tgcgtattcg tcagaaagca gaagttcacg cattgcgcga
     6181 accttgtctt taatcgtttt gaccggcttc gcttcgacga tagcaggtga cgcaacgcga
     6241 acctctttcg tcaccggagc agcaggctgt gatgcggcgg aaaatttcaa catacgccgt
     6301 aaaatgtcgg atgcgctctc gccgatatgc ttagtgtggc tggcaatata gctgtagagt
     6361 tcatcatcaa cttcaatcgt tttcatctta atccagtaca gtgtcttagc tgaatacaag
     6421 tcgccaagag tatatgaata aatcccggca gcggatagcg tcaagcctgg cttgcagaaa
     6481 aactcggaat aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca taatacccta
     6541 acccgacaac gcgaaaaaaa gaactttgcc atgaaattga atatccgcgc gcaaactgca
     6601 caaaaccagc acaataattc tcccatcgtt cttgtccatg gtctgtttgg tagcctcgac
     6661 aaccttggcg tactggctcg cgatctggta aacgatcaca atatcatcca ggttgatatg
     6721 cgtaaccacg gtctttcacc gagagatccg gtaatgaatt acccggcgat ggcgcaggat
     6781 cttgttgata ctctggatgc acagcagatc gacaaagcaa catttatcgg tcactccatg
     6841 ggcggtaaag cggtaatggc acttacggca ctggcccgcg atcgcatcga taaactggtg
     6901 gcgatcgata tcgcaccggt cgactatcac gtacgccgtc atgatgagat tttcgcggct
     6961 atcaacgcgg tcagtgaatc ggacgcacaa actcgccagc aagcagcagc aataatgcac
     7021 cagcatctta atgaagaagg ggtgattcag tttctgttga aatcttttgt tgacggggag
     7081 tggcgcttta acgtgccggt attgtgggat cagtatccgc atattgtggg ttgggagaaa
     7141 atcccggcat gggatcaccc tgccctgttt atccctggcg gcaattctcc gtatgttagc
     7201 gagcagtacc gtgatgattt actggcacaa tttccacagg cacgggcgca tgtgattgca
     7261 ggcgcgggtc actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc
     7321 tatctcaatg attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg
     7381 ggcgttggcg tgactcatcg cgctgatgta ttatggcgcg ctatccatcc gggcagaggc
     7441 ctggctgatt gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac
     7501 gacattagat ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta atccgctttc
     7561 gcgcgatgaa cagctgcgta ttaataaacg caaccagcta aaacgcgaca aagtacgtgg
     7621 ccttaagcgt gtcgaactga agctgaacgc ggaagctgtc gaggcgctga acgagctggc
     7681 ggagtcgcgc aatatgagcc gtagcgaact gatcgaagag atgttaatgc agcaactggc
     7741 ggcgctgcgt agtcagggca tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc
     7801 ctgctcgttt gctgattccg caagactgcc tgttctgcta tgattgcctt tatccgtggg
     7861 caattttcca cccccatttc aataagtttc aagaggttat ttcactcatg gctatcactg
     7921 gcatcttttt cggcagcgac accggtaata ccgaaaatat cgcaaaaatg attcaaaaac
     7981 agcttggtaa agacgttgcc gatgtccatg acattgcaaa aagcagcaaa gaagatctgg
     8041 aagcttatga cattctgctg ctgggcatcc caacctggta ttatggcgaa gcgcagtgtg
     8101 actgggatga cttcttcccg actctcgaag agattgattt caacggcaaa ctggttgcgc
     8161 tgtttggttg tggtgaccag gaagattacg ccgaatattt ctgcgacgca ttgggcacca
     8221 tccgcgacat cattgaaccg cgcggtgcaa cgatcgttgg tcactggcca actgcgggct
     8281 atcatttcga agcatcaaaa ggtctggctg atgacgacca ctttgtcggt ctggctatcg
     8341 acgaagaccg tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt
     8401 ctgaagagtt gcatctcgac gaaattctca atgcctgatg tgatgcggcg tagactcatg
     8461 tctacgccgt attaatagat aatgccaatc aaaataattg ctacaaattt gtaacttttg
     8521 ctgttgtacc tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa atgtaagctg
     8581 tgccacgttt ttattaacaa tatttgccag ggacttgtgg ttttcattta ggcgtggcaa
     8641 ttctataatg atacgcatta tctcaagagc aaattctgtc acttcttcta atgaagtgaa
     8701 ccgcttagta acaggacaga ttccgcatga ctgataacaa taccgcccta aagaaagctg
     8761 gcctgaaagt aacgcttcct cgtttaaaaa tcctagaagt tcttcaggag ccggacaacc
     8821 atcacgtcag tgcggaagat ttatacaaac gtctgatcga tatgggtgaa gaaattggtc
     8881 tggctacggt atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca
     8941 attttgaagg cggtaaatcc gtatttgaac tgacacagca acatcaccac gatcacctga
     9001 tctgcctcga ctgcggcaag gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc
     9061 gtgaaattgc cgcaaaacat ggcattcgcc tgactaacca cagtctctat ctttacggtc
     9121 actgtgccga aggcgattgc cgcgaagatg aacatgcgca cgaaggcaaa taagccagcc
     9181 tgaatgagaa aaagccaacc tgcgggttgg cttttttatg caagggaaat aagaaaagcg
     9241 agagttacag ctctcactta tttgttatta ctgcgaattt cctgccaaac tttatcacaa
     9301 tcggctttca ccgcctgatc atttccggtt tgcgttgcac gcagatactg ctgataatcc
     9361 agtacgcgga cactttcctg gtcggcaaaa tgctgatgtt gtttctcttt cttcagcacg
     9421 tttaacacgc tctggcaggc ttcaattttt tctggcgaac cttgtgcggt gttgatacag
     9481 gcgctgtatg cctctttcag acgactgtct tcctgtggcg cggttgattg cgcacaagct
     9541 accagccccg atgccattat ggcgatgaga atcagttttt tcatagcaaa ttcctcaaac
     9601 gtgcggcagg cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg gtgcgattac
     9661 cataaatttc acgtcacgct catcctggaa gatgttgccg taaccaccgc cccagcttgg
     9721 gatgtcggag tggttgtcgt attcggtgaa gtgcagtttg aacatcgtgc ctttggcgcg
     9781 accgtcctga atggtgtaga ccgcatccag gctgtatgca gactcttcaa tagtccggtt
     9841 tttgtcgtag tacgcatccg gattgctctg ctaggtcgca ggtttagcat cccatgcgta
     9901 aacgtaggaa gcgccgatgg cgaagcctgg aagattccag tttttcaggt catacatcgc
     9961 accgaagaag accgcttttt cgccgttagc gttgaagtcg gaacggttat cccaccagat
    10021 atccaggcgg ccgttggagg aagcgtaggt tggagtcata cgttgcagga agtatccctg
    10081 ctgaccgtca gccttaaccc aggtgccttc gaggcgcaaa tccactacgt cagccgcccg
    10141 gtaaccaaag gtcaacgctt gcagccaggc ggtgccgtca taaaggtcgt tgacgctgcg
    10201 atcgtcaact ttatcgcgcg taccatagaa ctggtagctg gtggttaacg ggctaccggc
    10261 gatatcaaat ttgtagctgg ctttggcaaa atattgatcg atataccctt ccgcctgacc
    10321 aaatgccgct tccagtacga agttattttt gaagtcgtat ttcgccccaa gggagtgcag
    10381 ataatcaact ttggtggttt tatcgttctg ataaaactca tccatttcca gatgccacgg
    10441 cgctttgtat tcgttggtcc acatgtagga gaaactcaac gcaccagcat cgccgtaatc
    10501 aaaattcgcc ccggcttccg ccccctgata agtacctggc ataaagctcc agtgcggcgc
    10561 taacagcgtt tgaccagttg gctgaatgta acctgccctc gcccaaaccg gaccgtattt
    10621 aaatttggcc gcagctttat acaggcttat accgcttttg tcgccggacc agtcttcgtc
    10681 ataggcttta ttactttttg aaaacgcgat ttcgttcggg tgggagctgt cgccgttttc
    10741 cgccatttca atcgccgtaa acgcagcaat atcaaggccg aacatatcag cagcataacc
    10801 ggactgaaaa tcgaggttgg cattccaggt ggagtgagaa aggttggttt tgtatttgtc
    10861 gccgtcggta acatctttac ggtcgcgttc acgctgccag taatatatac cgccggttaa
    10921 ggttgaatcg tcgatgaatc cttcggcgcg agcctccggc attgccataa agcccgacat
    10981 tgctgtaaca ccggcgatag ccagcgccag cgtactacgt ttgccactaa acgtacgcat
    11041 gggttaatcc tctttgacgt ataaattgct gcaccaaagg tgaacagcga aaaaataaaa
    11101 attaaaaaag tagcgttgag aaaagtctcg ctgactacag gaacagacta tttttcgcga
    11161 cgcgaattat caatctcttt ctgtaaccag aatgtaagaa catgagcaac cgcacatatt
    11221 ttgatgattt ttgttacaag gcttaagttt gcggttgaaa ggcttgcaga agaataaaag
    11281 cgtgaaattc cagcaagatg aaagcggtta cattttattt aattatgttt tgttatttta
    11341 aatttatttt atggataaat tgcgaagcgg aattaattcg cgaagaaaag cagaaaaaac
    11401 gccgctgaac gcggcgtttt ggattgtttg catatttctt gccggatgcg gcgtgaacgc
    11461 cttaaccggc ctacaaaagc acgcaaactc aatatattgc agagatcatg taggcctgat
    11521 aagcgtagtg catcaggcaa tttagcgttt gaaactgttt gtctgttcct tgccggatgc
    11581 gacgtaaacg ccccatccgg catagcgaaa cttaaaatta ctcgcctact ttcgcccagg
    11641 tatcacgcag cccaacggtg cggttaaata ccggtttttc cgccgtagaa tggcggctat
    11701 cgaggcagaa gtaaccttca cgctcaaact ggaatgcttt acccgcaacc gcatctttca
    11761 gcgacggttc agcaaagccc tgtttgatca ccagcgattc cgggttaatc accgacagga
    11821 aatcatccgc agcaccaggg ttcggcacgc tgaacagacg gtcgtacaga cggatttcaa
    11881 ccggcagcgc atgtgccgcg ctcacccagt gaataacacc tttgacttta cgaccatctg
    11941 ccggatcttt gcttaaggtg tcggcgtcat aagtacagaa gatggtggtg atattgcctt
    12001 cggcatcttt ctccacgcgt tctgccttaa tgacgtaagc attacgcaga cgcacttctt
    12061 tacccagcac cagacgtttg tactgcttgt tagcttcttc gcggaaatcg gcgcgatcga
    12121 tccaaatctc accgctaaac ggcacctggc ggctacccat ttccggtttg ttcggatggt
    12181 tcggcatggt aaccatttcg ccttcgccct gatagttttc gataaccagt ttcaccggat
    12241 cgataaccgc cattgcgcgc ggggcgtttt cgttgagatc ttcacggatg caggattcca
    12301 gcgacgccat ctcaatggtg ttgtcctgct tggtcacgcc gatgcgtttg cagaactcac
    12361 gaatagaagc agcagtgtaa ccacgacgac gcagaccgga aatggtcggc atacgcgggt
    12421 catcccagcc ttcaacgtgc ttgtcggtca ccagcaggtt cagcttacgc ttggacatca
    12481 cagtgtattc cagattcagg cgcgagaatt cgtactggcg cgggtgaaca ggaatggtga
    12541 tgttgtccag cacccagtcg tacaggcgac ggttgtcctg gaactcaagc gtacacagag
    12601 agtgcgtaat accttccagg gcatcgctga tgcagtgggt gaagtcgtac atcgggtaga
    12661 tgcaccactt gttgccagtc tggtggtgtt cagcaaattt aatgcggtac agcaccggat
    12721 cgcgcatcac gataaacggt gaagccatgt cgattttcgc acgcaggcag gctttacctt
    12781 cttcaaaacc accggcacgc attttttcga acagcgccag gttctcttca acgctgcggt
    12841 cgcggtacgg gctgttttta cccggttgcg tcagggtgcc gcggtattcg cggatctgtt
    12901 ccggcgtcag ttcatcaacg tacgccaggc ctttattgat cagttcgatc gcataggcgt
    12961 ggagctgatc aaaataatcg gaggagtaac ggacgttacc agaccagtga aaacctaacc
    13021 actctacgtc gtttttgatc gactcaacat actcgatatc ttcttttacc gggttagtgt
    13081 cgtcgaaacg caggttgcac tggcctttat agtcctgggc gatcccgaag ttcaggcaga
    13141 tagatttcgc atggccaata tgcagatagc cattcggctc cggcgggaaa cgggtgtgta
    13201 ctgtggtgtg cttaccactg gccagatctt catcgatgat ctgacggata aagttagtcg
    13261 ggcgggcttc tgcctcactc atcgtggatt cctcaaagcg taaacaacgt ataacggcgt
    13321 atgatcttat aagcgggaca ggctgacaac tgttagttag cgaaaatcga aaatctcact
    13381 gagattatgg ggatgattga tgaaaaaaaa aagcggcaga gaagatctcc accgcttttg
    13441 ccagtcccgc gtcattgttg gatgcgcgtc ttatccaacc aggactgagc ttttgtaggc
    13501 ctgataagac gcgtcaagcg tcgcatcagg cataaagcag attacttttt gatttcatac
    13561 agcggtgttt gacccgccac aacattgccc tgagctttaa tgatcaagcc actgaaatcg
    13621 tcgatattgc tgcaaaccac cgggctaatc atcgaacggg cgttagcgtt caggtaatcc
    13681 agatccattt ccagaatcgg ttgccctgcg ctcacctgcg cgccctcttc caccagacgt
    13741 ttaaagcctt taccttccag cgctacggtg tcgataccca tgtggacgac aatctccgcg
    13801 cctttttcgg tttctaggca gaacgcgtgg ttggtgttga agattttcac gattgtccct
    13861 gcggctggtg atacgacgat tttatctgtc ggtttcaccg ccacaccgtc acccaccgct
    13921 ttgctggcga atgcttcgtc agaaacctga tccagtgcca cgacatcacc ggtaatcggc
    13981 gataccagct ccgcgataga taccgcgttt ggtacagcct gcggttttgc tacaggagcg
    14041 gcagttgccg gagttgcttc agctgacgca gctgctaccg ggccacgggc aacgactttc
    14101 ttcatcgcat cgccaatgga ttctgctttc gcgccaacaa tcacctgaat ggtctgtttg
    14161 ttcagtttta ctacaccaga agcacccaga cgtttacaca tcgcatcgtt gacgcgagct
    14221 gagtcagcca cggtaaggcg cagacgggta atacaggcgt caatcgcttt caggttgtca
    14281 gtaccgccaa ccgcagcaat atagttggtt gccagttgag tcagaccttc ttcagtattg
    14341 ctgttagcct cttcagttac gatctcgtct tctttatctt cacgacccgg cgttttcagg
    14401 ttgaacatgc ggataaccaa actgaacacc acgaagtaga tagcgaagaa gacaacaccc
    14461 atcaccagca gcatccagac gttctggctg gcggccggca ggttatacat caacgcgtag
    14521 tcgatagccc ccgcagagaa agagaagcca gcatggatac ccagcagcgt tgccacaaac
    14581 aggctgatac cggtcagcag tgcgtgcagg aggtacagca gcggagcaag gaacatgaac
    14641 aggaattcca gcggctcagt cacaccggtc aggaacgcag taacagcaac agaaagcagc
    14701 atcccgccaa ccatcggacg acgctctttc ggtgctgcga agtacatcgc cagcgccgca
    14761 cccggcagac cgaacatcat gatcgggaag aagccggaca tgaacatccc cgcggtgccg
    14821 tcaccagcgt agaaacggtt gatgtcgccg tggaaaaccg tacccgccgc gttggtgaat
    14881 tcaccaatct ggaaccaggc gatggtgttc agcacctgat gcagaccggt tgggatcagc
    14941 agacggttga tgaaaccaaa gataccggaa cccagcgcgc ctgcagaaac gatccactcg
    15001 ccgcctgcat ggatagcgtg ctgtactggc ggccagacgt aaccaaaaat ggccgccagc
    15061 accaggcaga aaaagccggt ggcaatcggc acaaagcgtt tgccgccgaa gaagctcagg
    15121 aagtccggca gtttaatatc ggaccaacgg ttataggctg cgccaccaac cagaccggta
    15181 atgatacccg ccagtacacc catgttaatt tctgggttga tggtcaccat cgctttggtt
    15241 aacacaaagt aacctaccgc acccgccagc gccgccgcac ctgcgctgtc tttcgaccag
    15301 ctggatgcca caccgatggc gaagattaat gcgaggttat caaaaatcgc accgcccgcc
    15361 tgggcaataa acgcaacgtt aagtaaatct ggctgaccga atcgcagcaa cagtgccgcc
    15421 accggcagca ccgcgatagg gagctgtaac gccctaccga gtcgctggaa aaaacctaaa
    15481 atattcatct tattccccct acgagaaccc tatttggctc gtttaaagcc gtatttttat
    15541 tttgctgcaa attgtactgc cgatgttctg taatcagatt gttagatcat ctgctacaga
    15601 gtgtgtgaaa atttaattcg tatcgcaaat taaacgcgtg ccttttgtga gttttgtcac
    15661 caaatatcgt tatcatcact cccttttact ggctaaagca gaaaacttat tttatcattc
    15721 aaaaaatcag gtcggattga cgcctgtctg cgcaaatcca ggttacgctt aaagatgcct
    15781 aatccgccaa cggcttacat tttacttatt gaggtgaata atgagactga tccccctaac
    15841 taccgctgaa caggtcggca aatgggctgc tcgccatatc gtcaatcgta tcaatgcgtt
    15901 caaaccgact gccgatcgtc cgtttgttct gggcctgccg actggcggca cgccgatgac
    15961 cacctataaa gcgttagtcg aaatgcataa agcaggccag gtcagcttta agcacgttgt
    16021 caccttcaac atggacgaat atgtcggtct gccgaaagag catccggaaa gctactacag
    16081 ctttatgcac cgtaatttct tcgatcacgt tgatattcca gcagaaaaca tcaaccttct
    16141 caacggcaac gccccggata tcgacgccga atgccgccag tatgaagaaa aaatccgttc
    16201 ttacggaaaa attcatctgt ttatgggcgg tgtaggtaac gacggtcata ttgcatttaa
    16261 cgaaccggcg tcttctctgg cttctcgtac tcgtatcaaa accctgactc atgacactcg
    16321 cgtcgcgaac tctcgtttct ttgataacga tgtcaatcag gtgccaaaat atgccctgac
    16381 tgtcggtgtt ggtacattgc tggatgccga agaagtgatg attctggtgc tgggtagcca
    16441 gaaagcactg gcactgcagg ctgcagttga aggttgcgtg aaccacatgt ggaccatcag
    16501 ctgtctgcaa ctgcatccaa aagcgatcat ggtgtgcgat gaaccttcca ccatggagct
    16561 gaaagttaag actttaagat atttcaatga attagaagca gaaaatatca aaggtctgta
    16621 attgttatcc ctgccctggc tccttgctca gggcaatatt ttttaaaatc ggggggcaga
    16681 atgtatgcat taacccaggg ccggatcttt accggccacg aatttcttga tgaccacgcg
    16741 gttgttatcg ctgatggcct gattaaaagc gtctgtccgg tagcggaact gccgccagag
    16801 atcgaacaac gttcactgaa cggggccatt ctctcccccg gttttatcga tgtgcagtta
    16861 aacggctgcg gcggcgtaca gtttaacgac accgctgaag cggtcagcgt ggaaacgctg
    16921 gaaatcatgc agaaagccaa tgagaaatca ggctgtacta actatctgcc gacgcttatc
    16981 accaccagcg atgagctgat gaaacagggc gtgcgcgtta tgcgcgagta cctggcaaga
    17041 catccgaatc aggcgttagg tctgcatctg gaaggtccgt ggctgaatct ggtaaaaaaa
    17101 ggcacccata atccgaattt tgtgcgtaag cctgatgccg cgctggtcga tttcctgtgt
    17161 gaaaacgccg acgtcattac caaagtgacc ctggcaccgg aaatggttcc tgcggaagtc
    17221 atcagcaaac tggcaaatgc cgggattgtg gtttctgccg gtcactccaa cgcgacgttg
    17281 aaagaagcga aagccggttt ccgcgcgggg attacctttg ccacccatct gtacaacgcg
    17341 atgccgtata ttaccggtcg tgaaccgggc ctggcgggcg cgatcctcga cgaagctgac
    17401 atttattgcg gtattatcgc tgatggcctg catgttgatt acgccaacat tcgtaacgct
    17461 aaacgcctta aaggcgacaa actgtgtctg gttaccgacg ccaccgcgcc agcaggtgcc
    17521 aacattgaac agttcatttt tgcgggtaaa acaatatact accgtaacgg actttgtgtg
    17581 gatgagaacg gtacgttaag cggttcatcc ttaaccatga ttgaaggcgt gcgtaatctg
    17641 gtcgaacatt gcggtatcgc actggatgaa gtgctgcgta tggcgacgct ctatccggcg
    17701 cgtgcgattg gcgttgagaa acgtctcggc acactcgccg caggtaaagt agccaacctg
    17761 acctcattca cacctgattt taaaatcacc aagaccatcg ttaacggtaa cgaggtcgta
    17821 actcaataag agaaagtatg acaccaggcg gacaagctca gataggtaat gttgatctcg
    17881 taaaacagct taacagcgcg gcggtttatc gcctgattga ccagtacggg ccaatctcgc
    17941 ggattcagat tgccgagcaa agccagcttg cccccgccag cgtaaccaaa attacgcgtc
    18001 agcttatcga acgcgggctg atcaaagaag ttgatcagca ggcctccacc gggggccgcc
    18061 gcgctatctc catcgtcacc gaaacccgca atttccacgc aatcggcgta cggcttggtc
    18121 gtcacgacgc caccatcacc ctgtttgatc tcagcagcaa agtgctggca gaagaacatt
    18181 acccgctgcc ggaacgtacc cagcaaacgc tggaacatgc cctgttgaat gccattgctc
    18241 agtttattga tagctaccag cgcaaactgc gcgagctgat cgcgatttcg gtgatcctgc
    18301 cagggcttgt tgacccggac agcggcaaaa ttcattacat gccgcatatt caggtagaaa
    18361 actgggggct ggtagaagct ctggaagaac gttttaaagt gacctgtttc gttggtcacg
    18421 atatccgtag tctggcgctg gcggagcact acttcggtgc aagtcaggat tgcgaagact
    18481 ccattctggt gcgtgtccat cgcggaaccg gggccgggat tatctctaac gggcgcattt
    18541 ttattggccg caacggtaac gtcggtgaaa ttggccatat tcaggtcgaa ccgctgggtg
    18601 aacgctgcca ctgcggcaac tttggctgcc tggaaactat cgccgccaac gctgccattg
    18661 aacaacgggt gttgaatctg ttaaagcagg gctaccagag ccgcgtgccg ctggacgact
    18721 gcaccatcaa aactatctgc aaagccgcga acaaaggcga tagtctggcg tcggaagtaa
    18781 ttgagtatgt cggtcgtcat ctgggtaaaa ccatcgccat tgctatcaac ttatttaatc
    18841 cgcaaaaaat tgttattgcc ggtgaaatca ccgaagccga taaagtgctg ctccctgcta
    18901 ttgaaagctg cattaatacc caggcgctga aggcgtttcg tactaatctg ccggtggtac
    18961 gttctgagct ggatcaccgc tcggcaatcg gcgcttttgc gctggtaaaa cgcgccatgc
    19021 tcaacggtat tttgctccag catttgctgg aaaattaatg tgcttttata gtggcgctta
    19081 ttgttgtcaa tattctgggt agtccatgac cattaaaaat gtaatttgcg atatcgacgg
    19141 cgtgctgatg cacgataacg tcgccgtacc gggtgcagcg gaatttttgc acgggattat
    19201 ggataaaggc ctgccgctgg tgttgctgac caactatcct tcgcagaccg ggcaagatct
    19261 ggcgaaccgc tttgccactg ccggtgtcga tgtgccggat agcgtgtttt atacctctgc
    19321 gatggcgacc gccgatttcc tgcgtcgcca ggaaggcaag aaagcatatg tggtgggcga
    19381 aggcgcactg attcatgaac tgtacaaagc cggtttcact attaccgatg tgaaccctga
    19441 tttcgtgatt gttggcgaaa cgcgttccta caactgggac atgatgcata aagcggccta
    19501 tttcgtcgct aacggtgcac gctttatcgc caccaacccg gacacccacg ggcgcggttt
    19561 ttatcccgct tgtggcgcgt tgtgtgcagg gattgagaaa atctccgggc gcaaaccgtt
    19621 ctatgttggt aagcccagcc cgtggatcat ccgcgcagca ttaaacaaaa tgcaggcgca
    19681 ttcggaagaa acggtgattg tcggcgataa cttgcgcacc gacattctgg cgggcttcca
    19741 ggcaggtctg gaaacgattc tggtgctttc tggtgtttca tcgctcgacg atatcgacag
    19801 tatgccgttc cgcccaagct ggatttaccc gtcagtcgct gaaatcgacg ttatctgaat
    19861 accacccacg tctgcggacg tggttggctc tgatgacaaa cgccaaactg cctgatgcgc
    19921 tacgcttatc aggcctacgc agctcctgca atatattgaa ttttcatgct tttgtaggcc
    19981 ggataaggcg ttcacgccgc atccggcatg aacaaatcgc actttatcag caatttggc
//
LOCUS       DAZPJR010000068        19946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPJR010000068 DAZPJR010000000
VERSION     DAZPJR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.069"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            232..1326
                     /gene="gcvT"
                     /locus_tag="ACHVB7_002277"
     CDS             232..1326
                     /gene="gcvT"
                     /locus_tag="ACHVB7_002277"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR1914249.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            1350..1739
                     /gene="gcvH"
                     /locus_tag="ACHVB7_002278"
     CDS             1350..1739
                     /gene="gcvH"
                     /locus_tag="ACHVB7_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR1914250.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1858..4731
                     /gene="gcvP"
                     /locus_tag="ACHVB7_002279"
     CDS             1858..4731
                     /gene="gcvP"
                     /locus_tag="ACHVB7_002279"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR1914251.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            4997..5740
                     /locus_tag="ACHVB7_002280"
     CDS             4997..5740
                     /locus_tag="ACHVB7_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1914252.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5797..7230)
                     /gene="bglA"
                     /locus_tag="ACHVB7_002281"
     CDS             complement(5797..7230)
                     /gene="bglA"
                     /locus_tag="ACHVB7_002281"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR1914253.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            7275..7586
                     /gene="yqfB"
                     /locus_tag="ACHVB7_002282"
     CDS             7275..7586
                     /gene="yqfB"
                     /locus_tag="ACHVB7_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR1914254.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7750..8409
                     /locus_tag="ACHVB7_002283"
     CDS             7750..8409
                     /locus_tag="ACHVB7_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR1914255.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8605..9585)
                     /gene="ygfZ"
                     /locus_tag="ACHVB7_002284"
     CDS             complement(8605..9585)
                     /gene="ygfZ"
                     /locus_tag="ACHVB7_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR1914256.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9828..10094
                     /gene="sdhE"
                     /locus_tag="ACHVB7_002285"
     CDS             9828..10094
                     /gene="sdhE"
                     /locus_tag="ACHVB7_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR1914257.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10075..10482
                     /locus_tag="ACHVB7_002286"
     CDS             10075..10482
                     /locus_tag="ACHVB7_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR1914258.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10522..11043)
                     /gene="fldB"
                     /locus_tag="ACHVB7_002287"
     CDS             complement(10522..11043)
                     /gene="fldB"
                     /locus_tag="ACHVB7_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR1914259.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11155..12051
                     /gene="xerD"
                     /locus_tag="ACHVB7_002288"
     CDS             11155..12051
                     /gene="xerD"
                     /locus_tag="ACHVB7_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR1914260.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12076..12786
                     /gene="dsbC"
                     /locus_tag="ACHVB7_002289"
     CDS             12076..12786
                     /gene="dsbC"
                     /locus_tag="ACHVB7_002289"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR1914261.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12792..14525
                     /gene="recJ"
                     /locus_tag="ACHVB7_002290"
     CDS             12792..14525
                     /gene="recJ"
                     /locus_tag="ACHVB7_002290"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR1914262.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14616..15714
                     /gene="prfB"
                     /locus_tag="ACHVB7_002291"
     CDS             join(14616..14690,14692..15714)
                     /gene="prfB"
                     /locus_tag="ACHVB7_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR1914263.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15724..17241
                     /gene="lysS"
                     /locus_tag="ACHVB7_002292"
     CDS             15724..17241
                     /gene="lysS"
                     /locus_tag="ACHVB7_002292"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1914264.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17284..17832)
                     /gene="idi"
                     /locus_tag="ACHVB7_002293"
     CDS             complement(17284..17832)
                     /gene="idi"
                     /locus_tag="ACHVB7_002293"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR1914265.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17887..17958
                     /locus_tag="ACHVB7_002294"
     CDS             17887..17958
                     /locus_tag="ACHVB7_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914266.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17955..18080)
                     /gene="yqfG"
                     /locus_tag="ACHVB7_002295"
     CDS             complement(17955..18080)
                     /gene="yqfG"
                     /locus_tag="ACHVB7_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR1914267.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18082..19530)
                     /gene="uacT"
                     /locus_tag="ACHVB7_002296"
     CDS             complement(18082..19530)
                     /gene="uacT"
                     /locus_tag="ACHVB7_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR1914268.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         4897 a         5011 c         5348 g         4690 t
ORIGIN      
        1 atagaaactg gtgtgttttt tggtttttta ttctgtcgcg attttggcgt tttttaacca
       61 taagctaatg tgatgatcaa ttttacctta tggttaacag tctgtttcgg tggtaagttc
      121 aggcaaaaga gaacgattgc gttggggacc gggagtggct ccgatgctgg gtttcgtggt
      181 gataatttca ccatgaaaag ttgtcagccc cgcttattca atgaggacaa gatggcacaa
      241 cagactcctt tgtacgaaca acacacgctt tgcggcgctc gcatggtgga tttccacggc
      301 tggatgatgc cgctgcatta cggttcgcaa atcgacgaac atcatgcggt acgtaccgat
      361 gccggaatgt ttgatgtgtc acatatgacc atcgtcgatc tccgcggcag ccgcacccgg
      421 gagtttctgc gttatctgct ggcgaacgat gtggcgaagc tcaccaaaag cggcaaagcc
      481 ctttactcgg ggatgttgaa tgcctctggc ggtgtgatag atgacctcat cgtctactac
      541 tttactgaag atttcttccg cctcgttgtt aactccgcca cccgcgaaaa agacctctcc
      601 tggattaccc aacacgctga acctttcggc atcgaaatta ccgttcgtga tgacctttcc
      661 atgattgccg tgcaagggcc gaatgcgcag gcaaaagctg ccacactgtt taatgacgcc
      721 cagcgtcagg cggtggaagg gatgaaaccg ttctttggcg tgcaggcggg cgatctgttt
      781 attgccacca ctggttatac cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa
      841 gcggccgatt tctggcgtgc gctggtggaa gcgggtgtta agccatgtgg cttgggcgcg
      901 cgtgacacgc tgcgtctgga agcgggcatg aatctttatg gtcaggagat ggacgaaact
      961 atttctcctt tagccgccaa catgggctgg actatcgcct gggaaccggc agatcgtgac
     1021 tttatcggtc gtgaagccct ggaagtgcag cgtgagcatg gtacagaaaa actggttggt
     1081 ctggtgatga ccgaaaaagg cgtgctgcgt aatgaactgc cggtacgctt taccgatgcg
     1141 cagggcaacc agcatgaagg cattatcacc agcggtactt tctccccgac gctgggttac
     1201 agcattgcgc tggcgcgcgt gctggaaggt attggcgaaa cggcgattgt gcaaattcgc
     1261 aaccgtgaaa tgtcggttaa agtgacaaaa cctgtttttg tgcgtaacgg caaagccgtc
     1321 gcgtgattta cttttttgga gattgattga tgagcaacgt accagcagaa ctgaaataca
     1381 gcaaagaaca cgaatggctg cgtaaagaag ccgacggcac ttacaccgtt ggtattaccg
     1441 aacatgctca ggagctgtta ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa
     1501 cggttagcgc gggcgatgac tgcgcggttg ccgaatcagt aaaagcggcg tcagacattt
     1561 atgcgccagt aagcggtgaa atcgtggcgg taaacgacgc actgagcgat tccccggaac
     1621 tggtgaacag cgaaccgtat gcaggtggct ggatctttaa aatcaaagcc agcgatgaaa
     1681 gcgaactgga atcactgctg gatgcgaccg catacgaagc attgttagaa gacgagtaac
     1741 ggctttattc ctcttctgcg ggagaggatc agggtgagga aaatttatgc ctcaccctca
     1801 ctctcttcgt aaggagagag gttcacaatt cactgcacgt ttcaggaacc atcgctcatg
     1861 acacagacgt taagccagct tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg
     1921 gacgccgcgc aacagcaaga aatgctgaat gccgttggcg cacaatcgtt aaacgcgctg
     1981 accggccaga ttgtaccgaa agatattcag cttgcgaccc cgccgcaggt tggcgcaccg
     2041 gcgaccgaat acgccgcact ggcagaactc aaggctattg ccagtcgcaa taaacgcttc
     2101 acgtcttaca tcggcatggg ttacaccgcc gtgcagctac cgccggttat cctgcgtaac
     2161 atgctggaaa atccgggctg gtataccgcg tacactccgt atcaacctga agtctcccag
     2221 ggccgccttg aagcactgct caacttccag caggtaacgc tggatttgac tggactggat
     2281 atggcctctg cttctcttct ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg
     2341 aaacgcgtca gcaaactgaa aaatgccaac cgcttcttcg tggcttccga tgtgcatccg
     2401 caaacgctgg atgtggtccg tactcgtgcc gaaacctttg gttttgaagt gattgtcgat
     2461 gacgcgcaaa aagtgctcga ccatcaggac gtcttcggcg tgctgttaca gcaggtaggc
     2521 actaccggtg aaattcacga ctacactgcg cttattagcg aactgaaatc acgcaaaatt
     2581 gtggtcagcg ttgccgccga tattatggcg ctggtgctgt taactgcgcc gggtaaacag
     2641 ggcgcggata ttgtttttgg ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc
     2701 ccacacgcgg cattctttgc ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt
     2761 atcggtgtat cgaaagatgc agctggcaat accgcgctgc gcatggcgat gcagactcgc
     2821 gagcaacata ttcgtcgtga gaaagcgaac tccaacattt gtacttccca ggtactgctg
     2881 gcaaacatcg ccagcctgta tgccgtttat cacggcccgg ttggcctgaa acgtatcgct
     2941 aaccgcattc accgtctgac cgatattctg gcggcgggcc tgcaacaaaa aggtctgaaa
     3001 ctgcgccatg cgcactattt cgacaccttg tgtgtggaag tggccgacaa agcgggcgta
     3061 ctgacgcgtg ccgaagcggc tgaaatcaac ctgcgtagcg atatcctgaa cgccgtgggg
     3121 atcacccttg atgaaaccac cacgcgtgaa aacgtaatgc agcttttcag cgtgctgctg
     3181 ggcgataacc acgggctgga gatcgacacg ctggacaaag acgtggctca cgacagccgc
     3241 tctatccagc ctgcgatgct gcgcgacgac gaaatcctca cccatccggt gtttaatcgc
     3301 taccacagcg aaaccgaaat gatgcgctat atgcactcgc tggagcgtaa agatctggcg
     3361 ctgaatcagg cgatgatccc gctgggttcc tgcaccatga aactgaacgc cgccgccgag
     3421 atgatcccaa tcacctggcc ggaatttgcc gaactgcacc cgttttgccc gccggaacag
     3481 gctgaaggtt atcagcagat gattgcgcag ctggctgact ggctggtgaa actgaccggt
     3541 tacgacgccg tttgtatgca gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg
     3601 gcgattcgtc attatcatga aagccgcaac gaagggcatc gcgatatctg cctgatcccg
     3661 gcttctgcgc acggaactaa ccccgcttct gcacatatgg caggaatgca ggtggtggtt
     3721 gtggcgtgtg ataaaaacgg caacatcgat ctgactgatc tgcgcgcgaa agcggaacag
     3781 gcgggcgata acctctcctg tatcatggtg acttatcctt ctacccacgg cgtgtatgaa
     3841 gaaacgatcc gtgaagtgtg tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat
     3901 ggcgcgaaca tgaacgccca ggttggcatc acctcgccgg gctttattgg cgcggacgtt
     3961 tcgcacctca acctgcataa aactttctgc attccgcacg gcggtggtgg tccgggtatg
     4021 ggaccgatcg gtgtgaaagc gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa
     4081 atcgaaggca tgttaacccg tcagggcgca gtttctgcgg caccgttcgg tagcgcctct
     4141 atcctgccaa tcagctggat gtacatccgc atgatgggcg cagaagggct gaaaaaagca
     4201 agccaggtgg caatcctcaa cgccaactat attgccagcc gcctgcagga tgccttcccg
     4261 gtgctgtata ccggtcgcga cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg
     4321 ctgaaagaag aaaccggcat cagcgagctg gatattgcca agcgcctgat cgactacggt
     4381 ttccacgcgc cgacgatgtc gttcccggtg gcgggtacgc tgatggttga accgactgaa
     4441 tctgaaagca aagtggaact ggatcgcttt atcgacgcga tgctggctat ccgcgcagaa
     4501 attgaccagg tgaaagccgc tgtctggccg ctggaagata acccgctggt gaacgcgccg
     4561 cacattcaga gcgaactggt cgccgagtgg gcgcatccgt acagccgtga agttgcggta
     4621 ttcccggcag gcgtggcaga caaatactgg ccgacggtga aacgtctgga tgatgtttac
     4681 ggcgaccgta acctgttctg ctcctgcgta ccgattagcg aataccagta attcactgat
     4741 tcgactattt tctaaaggcg cttcggcgcc tttttagtca gatgacaaag tacaaaagtg
     4801 ctcagacagt cccctcgccc ctttggggag agggttaggg tgaggggaac aggccggcac
     4861 tggcgcgaat atttaccctc accccggccc tctccctgaa agggcgaggg gaaaagcgtg
     4921 ccaacattga agattgagcc agtttgttag caatctcaaa gatacgtcaa cgaattaatt
     4981 tttctcggaa aaacaaatgg ctatagcact tgtgaccgat ggcagtcgcg gcatcgggcg
     5041 ggcaactgca ttactgttgg cgaaagaaga gtatacggtg gcggttaatt atcaacaaaa
     5101 cctccatgcg gcgcaggaag tggtgaactt aatcacgcaa gccggtggca aggcattcgt
     5161 gctccaggcg gatatcagcg acgaaaatca ggtcatcgca atgtttacag caatcgatca
     5221 gcacgatgaa ccgctagcag cgctggtcaa taacgccggg atcttgttta cccagtgcac
     5281 cgttgaaaac cttaccgcag agcgaatcaa ccgagtactt tccaccaaca tgacgggata
     5341 ttttctctgc tgccgcgagg cggtaaaacg catggcgctt aaaaatggtg gcagtggcgg
     5401 cgctatcgtc aatgtctttt cggtggcctc acggttgggt tcgccagggg aatatgttga
     5461 ttacgcggca tcgaaagggg cgattgatac gttaaccacc ggactatcgc tggaagtcgc
     5521 cgcgcagggg gtccgcgtta actgcgtgcg gccagggttt atttttaccg aaatgcacgc
     5581 cagcggcggc gagcctggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg
     5641 tggacaggca gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta
     5701 cgtcacggga agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc
     5761 ggatgcaaca agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggt
     5821 aatcacctct ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc
     5881 gtcgtcatgt ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtggtgaa
     5941 cgatacgcag tcgatgcagc cccacggtgt gtagcccatc agatccacgc catcgtaagt
     6001 caccgctttt ttcatctctt cgatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt
     6061 gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag
     6121 cggcctctga taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat
     6181 ctgccagccc cagtcagacg ctttaacata cgggttcggt acgctgcctt cgaaaccaga
     6241 gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc
     6301 aagataatcg caggtgcctt cgcgcagtac atcgagatcg ccgtcttcca ttttgatgtt
     6361 aaatccgcga cgctcccact cgttcaacac ataggacggg taatagccgc gtagctgcac
     6421 atcggtaaag acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt
     6481 acagcagtag ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt
     6541 aatgcgacgc gcagctttca ccgccagggc gctggcgaca aactggtgat gcagcacctg
     6601 atacatcgtc tcttccgggt tctcatgctc ggtataaacc acgccggagc agcagtaacc
     6661 gaacagcggt gcacgccagt tacgctggtt gttaatttcg ttgaaggtca tccagtattt
     6721 gactttatgc ttatagcgtt caaacaccac ttccgcgaaa cggacaaaga aatcaaccac
     6781 tttacggttg gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga
     6841 gagggtgatc accggttcga tgttgtattt cagcagttca tcgaacatat catcgtagaa
     6901 cttcagccct tcttcgtttg gctgagcttc atcgcctttt ggaaaaatgc gcgtccaggc
     6961 tatggaagtc cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta
     7021 gtgaccataa aaatcaacgg cttcatggtt tggatagtat ttgcctggca agacttcttt
     7081 ggtgatttcg cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa tgctcggccc
     7141 tttgccgcct ttgttccagc cgccttcgac ctgatgagcg gcaactgcgc cgccccataa
     7201 gaaatctttc tgtaaggtga gttttttcac tatcataggc tccagatgag tcaattctta
     7261 caggaaatag taccatgcag ccaaacgaca tcactttttt tcaacgtttc caggatgaca
     7321 ttctggctgg gcgtaaaacc atcaccatcc gcgacgagtc tgaatcgcac ttcaaaacgg
     7381 gtgatgtgct tcgtgtcggg cgttttgaag atgacggtta tttttgcacg attgaagtca
     7441 ccgcaacctc aaccgtaacg ctggatacgc tgacggaaaa acatgcagag caggaaaata
     7501 tgaccctgac tgaactgata aaggtcattg ccgacatcta tcccggtcag acacagtttt
     7561 atgtgattga atttaaatgt ctttaaattg aactcacgcc tgttagttga aatttcgcat
     7621 ttaatttcat gatttttagg agattagtct caaatcagaa tttattttag ctaacaggtg
     7681 ttcactggaa ctattctcag ttacgctgga gaggtataca tgtgcgtgta cgtttctccg
     7741 gagtaagtta tggttcagaa gcccctcatt aagcagggat attcgctggc agaggaaatt
     7801 gccaacagcg tcagtcacgg cattgggttg gtgtttggta tcgttgggct ggtgttgcta
     7861 ctggttcagg cggtggatct caatgccagc gccacagcga taaccagtta cagcctctat
     7921 ggcggcagta tgatcctgct gttcctcgct tcgacgctct atcacgccat tcctcatcaa
     7981 cgggcaaaaa tgtggctgaa gaaatttgac cattgcgcta tttacctgtt gattgccgga
     8041 acctacacgc cgtttttgct ggtggggctg gattctccgt tagcgcgcgg gttgatgatt
     8101 gttatctgga gcctggcatt gctgggtatt ctgtttaaac tgaccatcgc gcaccgattc
     8161 aaaattttat ctctggtgac ctatctggcg atgggctggc tgtcgctggt ggtaatttat
     8221 gaaatggcag ttaagctcgc ggcgggcagc gttaccttat tggcggtagg cggcgtggtt
     8281 tattcgctcg gagtgatttt ctatgtctgc aaacgcattc cctacaatca tgccatctgg
     8341 cacggcttcg tgctcggcgg cagtgtgtgc cactttctgg cgatctattt gtatattggg
     8401 caggcgtaat gggtatgcct ttgtaggcct gaaaagacgc gtcagcgtcg catcaggcat
     8461 cggagcacaa ctgccggatt cggcgtgaac gccttatccg atctacgggc ctcgtccctg
     8521 ttgtgataaa ggcatcaaat cccagacatg ataagacgcg tcagcgtcgc atcaggcgat
     8581 aaatgcctga tgcgataaag agatttactc ttcgagcgaa tacggcagcg gctcaatatg
     8641 caatgtattc gcatcgtcgc gtacgcggaa gatgctatcc ggttccatat cgttattcat
     8701 gacgacctgt accacgacct gaccatcttc cagttttacc gcagccagca ccgtaccggt
     8761 acgacgccag ttctcgccca ttttcagctc taagtcttca ccagcttccg gcagtcggct
     8821 ggcgctacct gccagcaacc agagcgcacg tttattggca ccacggaatt ttgctcgcgc
     8881 caccatctct tgtccggtat aacagccttt cttaaagctg ataccgccca gcgcctggag
     8941 attggtcgcc tgtgggataa actgcccgct gttggcggca tcaatcaccg ggaaacccgc
     9001 ttcaatgttt aatgccagcc actgttggct attgttcagt tccgcttcac cgcgcagttt
     9061 atcggtcagc atattagcag tagcttcatc ggttacgatc aggaaacgtt ctgccgggta
     9121 ttcaaaccat agcaaagtgg tcgcgccttc tttaactacc tgtttttctt tcgaaggcag
     9181 ttcactaaag atatttgcca gcgcggcgcg cgcctgaaaa ccggcaacac caagcagcac
     9241 atgctcgtcg tctggcgcga tggtcacttt agagaacacc gcatattttt tcagttcagt
     9301 cagctgcggt tcacgcacgc tgcgccgttc aatccacgca aagccatcgc cgtcgcggaa
     9361 cagacgtaaa ttgctccaca ttttgccttt ggcgtcgcaa tgggcggcga gcaggtgctg
     9421 atcttctgcc atctggctga catctgctgt cacctgaccc tgcatatatt tttcgctgtc
     9481 cgcgccagta atggtggcaa gcgcccagtc atcaagcgtc atcagcgtca gcggtaaacg
     9541 ggcagaagcc gtaggctgac ggggaggaaa aggtgtaaaa gccataatag agtcctgagt
     9601 tgcttaacgg cgtattaatg actaatggta aaagagctat gaggcaatgc aagtgctttc
     9661 actacgcgat ctgtgccttc tgcgaggcga cttccaaaaa aatgaagcca ttgaattgtt
     9721 gtgaattaat ttcgtgatcg cgatggtgtt tactgatatt cccgtaaaag aaggcgaaaa
     9781 acacgttaca atagcggggt actggatttt cgtgagacag gaagaagatg gacattaaca
     9841 acaaagcccg cattcattgg gcatgccgcc gtggtatgcg cgaactcgat atttcaatca
     9901 tgccgttttt cgaacatgag tacgacagct taagcgatga cgaaaaacgc atctttattc
     9961 gtctgctgga atgtgacgat ccggacctgt ttaactggct gatgaatcac ggtaaaccgg
    10021 ccgatgcaga actggaaatg atggtccgac tcatccagac acggaaccgg gaacgtggtc
    10081 ctgtggcaat ctgatttgcg cgtctcctgg cgcgcacagt ggctttcctt gctgattcat
    10141 gggctggttg ccgctgttat tttactcatg ccctggccac tcagttacac cccgttatgg
    10201 atggtgttac tttcgctggt ggtgtttgat tgcgttcgca gccagcggcg tattaatgct
    10261 cgccaggggg aaattcgctt gttgatggac gggcgtttgc gttggcaagg gcaggagtgg
    10321 agcatcgtca aagcaccgtg gatgattaag agcggcatga tgctgcgttt acgttctgat
    10381 ggcggtaaac ggcaacattt atggctggca gccgacagca tggacgaagc cgaatggcgg
    10441 gatttacggc ggattttgtt gcaacaagag acgcaaagat aaagatgacg gcaaagcata
    10501 tgcgctaagc cgtcagtgag atcaggcgta atgctctgcc atttcgttga ggatttgctc
    10561 gcaccagctc tgaatacgct cgtcgctaag gtcatactgg ttagtttcat ccagcgccag
    10621 acccacgaac agttgcccgt cagcaatcac cggtttcggg ctggtaaatt catatccttc
    10681 cgttggccag tagccgacga acttcacgcc tttggtcgag agtttgtcat gcagcatacc
    10741 gagcgcatcg aggaaccact cgccgtatcc cagttgatcg ccaagcccat acagcgcaac
    10801 aattttacct tcaaggttca ggtcgtcgag ctgatcccag acggcttccc agtcttcctg
    10861 gatttcacca aaatcccagg tcgggatacc cagaatgagc acatcgtact gctccattaa
    10921 tttcggggag tcgtccttga ggttatgtaa ggtcaccagt tctgggccga taatatcgcg
    10981 gattttttct gccgccattt cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt
    11041 cataacgtaa gcttctcgat tctgctgtgg cttttgcagc tagtgtatca gaatcgcttt
    11101 agtcacggca caataaggca taatggatca aatgagtgac cataaggggc gcaagtgaaa
    11161 caggatctgg cacgcatcga gcagtttctt gatgctctgt ggctggaaaa aaatctggct
    11221 gaaaatacgt tgaacgctta ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac
    11281 cgcgggttga cgctggcgac ggcgcaaagt gacgatttgc aggcattact ggcagaacgg
    11341 ctggagggcg ggtataaagc caccagctca gcgcgtttgc tgagtgcagt gcgccgattg
    11401 ttccagtatc tttatcgcga aaagtttcgt gaagacgatc ccagtgcgca tctcgcttca
    11461 ccgaaattgc cccagcgttt gccaaaagat ttaagcgaag cgcaggtcga acgtttatta
    11521 caggcgccat taattgatca gccactggag ctacgcgata aagccatgct tgaagtgttg
    11581 tatgctaccg ggctgcgtgt ctctgaactg gtcgggctga caatgagtga tatcagcctg
    11641 cgtcagggcg tagtgcgggt cattggtaaa ggcaacaaag agcgcctggt accgttaggt
    11701 gaagaggcgg tttactggct ggaaacctat ctggaacatg ggcgtccgtg gctgctgaat
    11761 ggtgtgtcaa ttgacgtgtt gtttcccagc cagcgtgcgc agcagatgac gcgacagacc
    11821 ttctggcacc gcattaaaca ttatgccgtg ctggcgggta tcgacagcga aaagctgtca
    11881 ccgcatgtgt tgcgtcacgc ttttgccact catttattaa atcatggtgc ggatttacgc
    11941 gtggtgcaga tgctactggg ccacagcgat ctttccacca cgcaaattta tacgcatgtc
    12001 gctaccgagc gtctgcggca acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa
    12061 agaacgggaa gatttatgaa gaaaggtttt atgttgttta ctttgttagc ggcgttttca
    12121 ggttttgctc aggctgatga cgcggcaatt caacaaacgt tagccaaaat gggcatcaaa
    12181 agcagcgata ttcagcccgc gcccgtagct ggcatgaaga cagttctgac taacagcggc
    12241 gtgttgtaca tcaccgatga tggtaaacat atcattcagg ggccaatgta tgacgttagc
    12301 ggcacggctc cggtcaatgt caccaataag atgctgttaa agcagttgaa tgcgcttgaa
    12361 aaagagatga tcgtttataa agcgctgcag gaaaaacacg tcatcaccgt gtttactgat
    12421 attacctgtg gttactgcca caaactgcat gagcaaatgg cagactataa cgcgctgggg
    12481 atcaccgtgc gttatcttgc tttcccgcgc caggggctgg acagcgatgc agagaaagaa
    12541 atgaaagcta tctggtgtgc gaaagataaa aacaaagcgt ttgatgatgt gatggcaggt
    12601 aaaagcgtcg caccagccag ctgcgacgtg gatattgccg accattacgc acttggcgtc
    12661 cagcttggcg ttagcggtac tccggcagtt gtgctgagca atggcacact tgttccgggt
    12721 taccagccgc cgaaagagat gaaagaattc ctcgacgaac accaaaaaat gaccagcggt
    12781 aaataattcg cgtgaaacaa cagatacaac ttcgtcgccg tgcagtcgat gaaacggcag
    12841 acttgcccgc tgaattgcct cccttgctgc gccgtttata cgccagccgg ggcgtgcgca
    12901 gtgcgcaaga actggaacgc agtgttaaag ggatgctgcc ctggcagcaa ctgagcggcg
    12961 tcgaaaaggc cgttgagatc ctttacaacg cctttcgcga aggaacgcgg attattgtgg
    13021 tcggtgattt cgacgccgac ggtgccacca gcacggctct aagcgtgctg gcgatgcgct
    13081 cgcttggttg cagcaatatc gactacctgg ttccaaaccg tttcgaagac ggttacggct
    13141 taagcccgga agtggtcgat caggcccatg cccgtggtgc gcagttaatt gtcacggtgg
    13201 ataacggtat ttcctcccat gcgggggttg aacacgctcg ctcgttgggc attccggtta
    13261 ttgttaccga tcaccatttg ccgggcgaaa cattacccgc ggcggaagcg atcattaacc
    13321 ctaacttgcg cgactgtaat ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt
    13381 atctgatgct ggcgctgcgc acctttttgc gcgatcaggg ctggtttgat gagcgcggca
    13441 tcgcaattcc taacctggca gaactgctgg atctggtagc gctggggaca gtggcggacg
    13501 tcgtaccgct ggacgctaat aatcgcattc tgacctggca ggggatgagt cgcatccgtg
    13561 ccggaaagtg ccgtccgggg attaaagcgc tgcttgaagt ggcaaaccgt gatgcacaaa
    13621 aactcgccgc cagcgattta ggttttgcgc tggggccacg tctcaatgct gccggacgac
    13681 tggacgatat gtccgtcggt gtggcgctct tgctgtgcga caacatcggc gaagcgcgcg
    13741 tgctggcaaa tgaactcgat gcgctaaacc agacgcgaaa agagatcgaa caaggaatgc
    13801 aggttgaagc cctgaccctg tgcgagaaac tggagcgcag ccgtgacacg ctacccggcg
    13861 ggctggcaat gtatcacccc gaatggcatc agggggttgt cggtattctg gcttcccgta
    13921 ttaaagagcg ttttcaccgt ccggttatcg cctttgcgcc cgcgggtgat ggtacgctga
    13981 aaggttctgg tcgctccatt caggggctgc atatgcgtga tgcgctggag cgattagaca
    14041 cactctaccc tggcatgatg ctgaagtttg gcggtcatgc gatggcggcg ggtttgtcgc
    14101 tggaagcgga taaattcgaa ctctttcaac aacggtttgg cgaactggtt actgagtggc
    14161 tggacccttc gctattgcaa ggcgaagtgg tgtcagacgg tccgttaagc ccggccgaaa
    14221 tgaccatgga agtggcgcag ctgctgcgcg atgctggccc gtgggggcag atgttcccgg
    14281 agccgctgtt tgacggtcat ttccggctgc tgcaacagcg gctggtgggc gaacgtcatt
    14341 tgaaagtcat ggtcgaaccg gtcggcggcg gtccgctgct ggatggtatt gcttttaatg
    14401 tcgataccgc cctctggccg gataacggcg tgcgcgaagt gcaactggct tacaagctcg
    14461 atatcaacga gtttcgcggc aaccgcagcc tgcaaattat catcgacaat atctggccaa
    14521 tttagcgtca tcttctctat aaaaaagagc gtggattggg tacaatcccg ctcttatcac
    14581 cgcattttga ctagctcaat aaaagaaatc agaccatgtt tgaaattaat ccggtaaata
    14641 atcgcattca ggacctcacg gaacgctccg acgttcttag ggggtatctt tgactacgac
    14701 gccaagaaag agcgtctgga agaagtaaac gccgagctgg aacagccgga tgtctggaat
    14761 gaacccgaac gcgcacaggc gctgggtaaa gagcgttcct ccctcgaagc cgttgtcgac
    14821 accctcgacc aaatgaaaca ggggctggaa gatgtttctg gtctgctgga actggctgta
    14881 gaagctgacg acgaagaaac ctttaacgaa gccgttgctg aactcgacgc cctggaagaa
    14941 aaactggcgc agcttgagtt ccgccgtatg ttctctggcg aatatgacag cgccgactgc
    15001 tacctcgata ttcaggcggg gtctggcggt acggaagcac aggactgggc gagcatgctt
    15061 gagcgtatgt atctgcgctg ggcagaatcg cgtggtttca aaactgaaat catcgaagag
    15121 tcggaaggtg aagtggcggg tattaaatcc gtgacgatca aaatctccgg cgattacgct
    15181 tacggctggc tgcgtacaga aactggcgtt caccgcctgg tgcgtaagag cccgtttgac
    15241 tccggcggtc gtcgccacac gtcgttcagc tctgcgtttg tttatccgga agttgatgat
    15301 gatattgata tcgaaatcaa cccggcggat ctgcgcattg acgtttatcg cgcgtccggc
    15361 gcgggcggtc agcacgttaa ccgtaccgaa tctgcggtgc gtattaccca catcccgacc
    15421 gggatcgtga cccagtgcca gaacgaccgt tcccagcaca agaacaaaga tcaggccatg
    15481 aagcagatga aagcgaagct ttatgaactg gagatgcaga agaaaaatgc cgagaaacag
    15541 gcgatggaag ataacaaatc tgacatcggc tggggcagcc agattcgttc ttatgtcctt
    15601 gatgactccc gcattaaaga tctgcgtacc ggggtagaaa ctcgcaacac gcaggccgtg
    15661 ctggacggca gcctggatca atttatcgaa gcaagtttga aagcagggtt atgaggaacc
    15721 aacatgtctg aacaacacgc acagggcgct gacgcggtag tcgatcttaa caatgaactg
    15781 aaaacgcgtc gtgagaagct ggcgaacctg cgcgagcagg ggattgcctt cccgaacgat
    15841 ttccgtcgcg atcatacctc tgaccaattg cacgcagaat tcgacggtaa agagaacgaa
    15901 gaactggaag cgctgaacat cgaagtcgcc gttgctggcc gcatgatgac ccgccgtatt
    15961 atgggtaaag cgtctttcgt taccctgcag gatgtgggtg gccgcattca gctgtacgtt
    16021 gcccgtgacg atctgccgga aggcgtttac aacgagcagt tcaaaaaatg ggacctcggc
    16081 gacatcctcg gcgcgaaagg taaactgttc aaaaccaaaa ccggcgaact gtctatccac
    16141 tgtaccgagc tgcgtctgct gaccaaagca ctgcgtccgc tgccggataa attccacggc
    16201 ttgcaggatc aggaagcgcg ctatcgtcag cgttatctgg atctcatctc taacgatgaa
    16261 tcccgtaaca cctttaaagt acgctcgcag atcctctctg gtattcgcca gttcatggtg
    16321 aaccgcggct ttatggaagt tgaaacgccg atgatgcagg tgatcccagg cggtgccgct
    16381 gcgcgtccgt ttatcaccca ccataacgcg ctggatctcg acatgtacct gcgtatcgcg
    16441 ccggaactgt acctcaagcg tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac
    16501 cgtaacttcc gtaacgaagg tatttccgta cgtcataacc cagagttcac catgatggaa
    16561 ctctacatgg cttacgcaga ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt
    16621 actctggcac aggatattct cggtaagacg gaagtgacct acggcgacgt gacgctggac
    16681 ttcggtaaac cgttcgaaaa actgaccatg cgtgaagcga tcaagaaata tcgcccggaa
    16741 accgacatgg cggatctgga taacttcgac tctgcgaaag cgattgctga atctatcggt
    16801 atccacgttg agaagagctg gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg
    16861 gcagaagcac atctgattca gccgaccttc attactgaat atccggcaga agtttctccg
    16921 ctggcgcgtc gtaacgacat taacccggaa atcacagacc gctttgagtt cttcatcggt
    16981 ggtcgtgaaa tcggtaacgg ctttagcgag ctgaatgacg cggaagatca ggcgcaacgc
    17041 ttcctggatc aggttgccgc gaaagacgca ggtgacgacg aagcgatgtt ctacgatgaa
    17101 gattacgtta ccgcactgga acatggctta ccgccgacag caggtctggg gattggcatc
    17161 gaccgtatgg taatgctgtt caccaacagc cataccatcc gcgacgttat tctgttcccg
    17221 gcgatgcgtc cggtaaaata agcattacgt tatgctcaca accccggcaa atgtcggggt
    17281 tttttaattg tgctgcgcga aagcagacaa caattttctt gcttcactat tggctgcctg
    17341 catcaccatc cacggactga acgcccacgg tgtggcatca ataccgtgta atacatctgc
    17401 taaatcacac cattgataat ccatcacttc atcatcgttg atctgtaacg cactggtggt
    17461 gcgtgcggca aataccggac acacttcatt ttccacaatg ccattcggat cggtggcgcg
    17521 atagcgaaag tcaggataga tagattcagg aggcgtaatt tccacgccaa gctcataacg
    17581 gcaacggcgg atcaccgcgt cttcgttgct ttctcccagt tgtgggtgcc cacaaaccga
    17641 gttagtccac acgccaggcc acgctttttt gctcagtgcg cggcgggtaa ctaataattg
    17701 tcctttggca ttaaacagcc aactggagaa cgcgagatgt aagcgggtgt ctgccgtgtg
    17761 tgcggcatac ttttccagcg tacccgtggg aactccctgt gcattcaata aaatgacgtg
    17821 ttccgtttgc ataatttctc acatgtaatt ctgatcactc atcattatag ataattgaag
    17881 agtgaaatga ttaaccaagt gagcgtttat cgacaaccgc ccgttttgag cggatgccga
    17941 caggtaaaaa ccatttaaaa aagtagcatc atatatttga agcgactttc aatggccatt
    18001 gcaacacagt cagaaatgac aggaatagag agagtaaaaa gcaacagcag actgaatata
    18061 acgcgcatta aaaaattcat attattctcc atgctcattt ttcttcagct tgcgcatcag
    18121 aagccacata cgtactgtcc tgacttttaa atctttatca gagacggatt ccttcaccag
    18181 gtcagcatga tgctggtagc cattaaagaa gacattcagc acaacggcgc taagggttgc
    18241 gagcataatg ccgctatgca gcaacggttg cagtacggcc ggtaacttag aaaagaaatc
    18301 gtgagagagc gtcggagtca tgccaacgcc gagactgatc gccacaatat agaggttgta
    18361 acggttggtg gtgtagttac agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc
    18421 gaacatcacc agcccagcac cgcccagcac aaattgcgga atggaggcga ccagcaccgc
    18481 catttttggc accatgccaa acaggattaa tataattccc gaagaaatac acacccagcg
    18541 gctatgaacg cgcgtcacgc taaccaggcc aacgttttgt gaaaatgacg tgtggaggaa
    18601 gctgttaaac gttccgccta tcattgtccc tacgccatcg acacgcagcc cgcgaataat
    18661 atcgtgtgaa gagagtttac gaccgactat ttcacccagt gccaggaaca tccccatcga
    18721 ctcgataaac acgatgatta acacggcagt catggtcaga atggaaacgg gatcgaaaat
    18781 cggcatacca aatgacatcg gcgtgacaat cgcaaaccat gaagcatcat gtagcccgga
    18841 taaattgact tcattcatca tccacgaaag taaaaagcca aatacaatcc ccagtaatac
    18901 ggcgacgttg gacataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac
    18961 agcaaaggag atacctaaat aaacgggatt accatattgc ggattccctt tacctccggc
    19021 ggcccagtca atacccacct gaatgatgct aagcccaata gaagtaataa ccacaccggt
    19081 aaccagtggc gggaataaag gcatcaagcg accaataaat ggcgctaata atgtggtgat
    19141 aaaacccgcg gcgatagtgg caccaaatat ccccagcagg ccgatatccg ggttcatccc
    19201 aatggctatc attggtgtta cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat
    19261 ccccataaag cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag
    19321 atccgagcta atgagcatcg caatagcatc ttttgagagg cccagtcggt caccaatcat
    19381 aagaggaaca gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat
    19441 cagctttcct ggtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga
    19501 tgagggaagt tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagagtaa
    19561 aaagcaaccg tttacgttaa tgaaactgtc taaacacatg ctcgtggagt gccagttttt
    19621 gcgaggaagg ggagttagag acaggaattg cggaggcaat cacataatcg tgcttaaacc
    19681 acgaaaatga caagagaacc tgacaatgga attatctgta attttctcat attgatattt
    19741 cattaatcat tctggcatgt cgcactctcg catttaatcg tttttatctg gatagcgctc
    19801 ttttgatcgg caatgttgaa ataatgaaag cggcatagat tgaggggcaa taatttgaac
    19861 tcagtcgcaa aattataatt aaaacggtca tgctacctaa tatatgcccg tttattgcat
    19921 cgcgaaattt gagaagacat aactca
//
LOCUS       DAZPJR010000069        19408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPJR010000069 DAZPJR010000000
VERSION     DAZPJR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.070"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHVB7_002297"
     CDS             <1..245
                     /locus_tag="ACHVB7_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR1914269.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHVB7_002298"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHVB7_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR1914270.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHVB7_002299"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHVB7_002299"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR1914271.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHVB7_002300"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHVB7_002300"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR1914272.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHVB7_002301"
     CDS             3423..4061
                     /locus_tag="ACHVB7_002301"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR1914273.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHVB7_002302"
     CDS             4066..4842
                     /locus_tag="ACHVB7_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR1914274.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHVB7_002303"
     CDS             4931..6295
                     /locus_tag="ACHVB7_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR1914275.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALCASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHVB7_002304"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHVB7_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR1914276.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHVB7_002305"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHVB7_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR1914277.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHVB7_002306"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHVB7_002306"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR1914278.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHVB7_002307"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHVB7_002307"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR1914279.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHVB7_002308"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHVB7_002308"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR1914280.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHVB7_002309"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHVB7_002309"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR1914281.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHVB7_002310"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHVB7_002310"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR1914282.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHVB7_002311"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHVB7_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR1914283.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHVB7_002312"
     CDS             complement(12844..13167)
                     /locus_tag="ACHVB7_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR1914284.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHVB7_002313"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHVB7_002313"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR1914285.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHVB7_002314"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHVB7_002314"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR1914286.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHVB7_002315"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHVB7_002315"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR1914287.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHVB7_002316"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHVB7_002316"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR1914288.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2e"
                     /locus_tag="ACHVB7_002317"
     CDS             complement(17716..18009)
                     /gene="cas2e"
                     /locus_tag="ACHVB7_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR1914289.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="ACHVB7_002318"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="ACHVB7_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(18926..>19408)
                     /gene="cas6e"
                     /locus_tag="ACHVB7_002319"
     CDS             complement(18926..>19408)
                     /gene="cas6e"
                     /locus_tag="ACHVB7_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR1914290.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4592 a         5230 c         4848 g         4738 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgtgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagcg
//
LOCUS       DAZPJR010000070        18843 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPJR010000070 DAZPJR010000000
VERSION     DAZPJR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.071"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /locus_tag="ACHVB7_002320"
     CDS             complement(<1..101)
                     /locus_tag="ACHVB7_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914291.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHVB7_002321"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHVB7_002321"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1914292.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHVB7_002322"
     CDS             complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHVB7_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR1914293.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHVB7_002323"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHVB7_002323"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR1914294.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHVB7_002324"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHVB7_002324"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR1914295.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHVB7_002325"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHVB7_002325"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR1914296.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHVB7_002326"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHVB7_002326"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1914297.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHVB7_002327"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHVB7_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR1914298.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="ACHVB7_002328"
     CDS             9871..10449
                     /locus_tag="ACHVB7_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1914299.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="ACHVB7_002329"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="ACHVB7_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR1914300.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12429..13376
                     /gene="cyoA"
                     /locus_tag="ACHVB7_002330"
     CDS             12429..13376
                     /gene="cyoA"
                     /locus_tag="ACHVB7_002330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR1914301.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13398..15389
                     /gene="cyoB"
                     /locus_tag="ACHVB7_002331"
     CDS             13398..15389
                     /gene="cyoB"
                     /locus_tag="ACHVB7_002331"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR1914302.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15379..15993
                     /locus_tag="ACHVB7_002332"
     CDS             15379..15993
                     /locus_tag="ACHVB7_002332"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR1914303.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15993..16322
                     /locus_tag="ACHVB7_002333"
     CDS             15993..16322
                     /locus_tag="ACHVB7_002333"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR1914304.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16334..17224
                     /gene="cyoE"
                     /locus_tag="ACHVB7_002334"
     CDS             16334..17224
                     /gene="cyoE"
                     /locus_tag="ACHVB7_002334"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR1914305.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17409..17720
                     /locus_tag="ACHVB7_002335"
     CDS             17409..17720
                     /locus_tag="ACHVB7_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1914306.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17704..18246
                     /locus_tag="ACHVB7_002336"
     CDS             17704..18246
                     /locus_tag="ACHVB7_002336"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1914307.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18302..>18843)
                     /locus_tag="ACHVB7_002337"
     CDS             complement(18302..>18843)
                     /locus_tag="ACHVB7_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR1914308.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4217 a         4953 c         4580 g         5093 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cagcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagtt cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 cttgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctgaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcac tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
    12001 cgccgtaatt attacggcta aataaacatc agtatcgctt aataatattt gttcttttgt
    12061 gcggcttagc gtttggaaat tattgacgca atttatgaat tctatttttt cttacacgat
    12121 tcagctaatg agtctttatt ttccccccac ccaattgtca ccataatgat aattatttgt
    12181 taaacaattg ttttattcaa gattggttat accaattgcc cgtccaaact ccttctgcac
    12241 tcccctttcg ttataacgcc cttttgcaac agcttcttaa aatcaacttg atatgttttg
    12301 caacatatgt aacctgacag ccaaatccaa gtaacaggaa tttaatcatg tttacagtaa
    12361 tgtaacctcc ccgtaaaatg cccacacact ttaaacgcca ccagatcccg tggaattgag
    12421 gtcgttaaat gagactcagg aaatacaata aaagtttggg atggttgtca ttatttgcag
    12481 gcactgtatt gctcagtggc tgtaattctg cgctgttaga tcccaaagga cagattggtc
    12541 tggagcaacg ttcactgata ctgacggcat ttggcctgat gttgattgtc gttattcccg
    12601 caatcttgat ggctgttggt ttcgcctgga agtatcgtgc gagcaataaa gatgctaagt
    12661 acagcccgaa ctggtcacac tccaataaag tggaagctgt ggtctggacg gtacctatct
    12721 taatcatcat cttccttgcg gtactgacct ggaaaaccac tcacgctctt gagcctagca
    12781 agccgctggc acacgacgag aagcccatta ccatcgaagt ggtttccatg gactggaaat
    12841 ggttcttcat ctacccggaa cagggcattg caaccgtgaa tgaaatcgct ttcccggcga
    12901 acactccggt gtacttcaaa gtgacctcca actccgtgat gaactccttc ttcattccgc
    12961 gtctgggtag ccagatttat gccatggccg gtatgcagac tcgcctgcat ctgatcgcca
    13021 acgaacccgg tacttatgac ggtatctccg ccagctacag cgggccgggc ttctcaggca
    13081 tgaagttcaa agctattgca acaccggatc gcgccgaatt cgaccaatgg gtcgcaaaag
    13141 cgaaacagtc gccgaacacc atgtctgaca tggctgcgtt cgaaaaactg gccgcgccta
    13201 gcgaatacaa ccaggtggaa tatttctcca acgtgaaacc agacttgttt gccgatgtga
    13261 ttaacaagtt tatggctcac ggtaagagca tggacatgac ccagccagaa ggtgagcaca
    13321 gtgcacacga aggtatggaa ggcatggaca tgagccacgc ggaatccgcc cattaaaggg
    13381 gttgaggaag aataaagatg ttcggaaaat tatcacttga tgcagtcccg ttccatgaac
    13441 ctatcgtcat ggttacgatc gctggcatta ttttgggagg tctggcgctc gttggcctga
    13501 tcacttactt cggtaagtgg acctacctgt ggaaagagtg gctgacctcc gtcgaccata
    13561 aacgcctcgg tatcatgtat atcatcgtgg cgattgtgat gttgctgcgt ggttttgctg
    13621 acgccattat gatgcgtagc cagcaggctc ttgcctcggc gggcgaagcg ggcttcctgc
    13681 cacctcacca ctacgatcag atcttcaccg cgcacggcgt gattatgatc ttcttcgtgg
    13741 cgatgccttt cgttatcggt ctgatgaacc tggtggttcc gctgcagatc ggcgcgcgtg
    13801 acgttgcgtt cccgttcctc aacaacttaa gcttctggtt tactgttgtt ggtgtgattc
    13861 tggttaacgt ttctctcggc gtgggcgaat ttgcgcagac cggctggctg gcctatccac
    13921 cgctatcggg aatagagtac agtccgggag tcggtgtcga ttactggata tggagtctcc
    13981 agctatccgg tataggtacg acgcttaccg gtatcaactt cttcgttacc attctgaaga
    14041 tgcgcgcacc gggcatgacc atgttcaaga tgccagtatt tacctgggca tcactgtgcg
    14101 cgaacgtact gattattgct tccttcccaa ttctgacggt taccgtcgcg ttgttgaccc
    14161 tggatcgcta tctgggcacc catttcttta ccaacgatat gggtggcaac atgatgatgt
    14221 acatcaacct gatttgggcc tggggccacc cggaagttta catcctgatc ctgcctgttt
    14281 tcggtgtgtt ctccgaaatt gcggcaacct tctcgcgtaa acgtctgttt ggttatacct
    14341 cgctggtatg ggcaaccgtc tgtatcaccg tgctgtcgtt catcgtttgg ctgcaccact
    14401 tctttacgat gggtgcgggc gcgaacgtaa acgccttctt tggtatcacc acaatgatta
    14461 tcgccatccc gaccggggtg aagatcttca actggctgtt caccatgtat cagggccgca
    14521 tcgtgttcca ttctgcgatg ctgtggacca tcggttttat cgtcactttc tcggtgggcg
    14581 ggatgactgg cgtgctgctg gcagtaccgg gcgcggactt cgttctgcat aacagcctgt
    14641 tcctgattgc gcacttccat aacgttatca tcggcggcgt ggtcttcggc tgcttcgcag
    14701 ggatgaccta ctggtggcct aaagcgttcg gtttcaaact gaatgaaacc tggggtaaac
    14761 gcgcgttctg gttctggatc atcggcttct tcgttgcctt tatgccgctg tatgcgctgg
    14821 gcttcatggg catgacccgt cgtttgagcc agcagattga cccgcagttc cacaccatgc
    14881 tgatgattgc agctagcggt gcagtactga ttgcgctggg tattctctgc ctcgttattc
    14941 agatgtacgt ttctattcgc gaccgcgacc agaaccgtga cctgactggc gacccgtggg
    15001 gtggccgtac gctggagtgg gcaacctctt ccccgcctcc gttctataac tttgccgttg
    15061 tgccgcacgt tcacgagcgt gatgcattct gggaaatgaa agagaaaggc gaagcgtata
    15121 aaaagcctga ccactatgaa gaaattcata tgccgaaaaa cagcggtgca ggtatcgtca
    15181 ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg gcatatctgg tggctggcga
    15241 ttgttggctt cgcaggcatg atcatcacct ggatcgtgaa aagcttcgac gaggacgtgg
    15301 attactacgt gccggtggca gaaatcgaaa aactggaaaa ccagcatttc gatgagatta
    15361 ctaaggcagg gctgaaaaat ggcaactgat actttgacgc acgcgactgc ccacgcgcac
    15421 gaacacgggc accacgatgc aggcggaacc aaaatcttcg gattttggat ctacctgatg
    15481 agcgactgca ttctgttctc aatcttgttt gctacctatg ccgttctggt gaacggcacc
    15541 gcaggcggcc cgacaggtaa ggacattttc gaactgccgt tcgttctggt tgaaactttc
    15601 ttgctgttgt tcagctccat cacctacggc atggcggcta tcgccatgta caaaaacaac
    15661 aaaagccagg ttatctcctg gctggcgttg acctggttgt ttggtgccgg atttatcggg
    15721 atggaaatct acgaattcca tcacctgatt gttaacggca tgggtccgga tcgcagcggc
    15781 ttcctgtcag cgttctttgc gctggtcggc acgcacggtc tgcacgtcac ttccggtctt
    15841 atctggatgg cggtgctgat ggtacaaatc gctcgtcgcg gcctgaccag cactaaccgt
    15901 acccgcatca tgtgcctgag cctgttctgg cacttcctgg atgtggtttg gatctgtgtg
    15961 ttcactgttg tttatctgat gggggcgatg taatgagtca ttctaccgat cacagcggcg
    16021 cgtcccatgg cagcgtaaaa acctacatga caggctttat cctgtcgatc attctgacgg
    16081 tgattccgtt ctggatggtg atgacaggag ctgcctctcc ggccgtaatt ctgggaacaa
    16141 tcctggcaat ggcagtggta caggttctgg tgcatctggt gtgcttcctg cacatgaata
    16201 ccaaatcaga tgaaggctgg aacatgacgg cgtttgtctt caccgtgcta atcatcgcta
    16261 tcctggttgt aggctccatc tggattatgt ggaacctcaa ctacaacatg atgatgcact
    16321 aagagcggcg gttatgatgt ttaagcaata cctgcaagta acgaaaccag gcatcatctt
    16381 tggcaacctg atctcggtga ttgggggatt cctgctggcc tcaaagggca gcattgatta
    16441 tcccctgttt atctacacgc tggttggggt gtcactggtt gtggcgtcgg gttgtgtgtt
    16501 taacaactac atcgacaggg atatcgacag aaagatggaa aggacgaaga atcgggtgct
    16561 ggttaaaggc ctgatctctc ctgctgtctc gctggtgtac gccaccttgc taggctttgc
    16621 tggctttatg ctgctgtggt ttggcgcgaa tccgctggcc tgctggctgg gggtgatggg
    16681 ctttgtggtt tatgtcggcg tttatagcct gtacatgaaa cgccactcgg tctacggcac
    16741 attgattggt tcgctctccg gcgctgcgcc gccggtgatt ggctactgtg cagtaaccgg
    16801 cgagttcgat agcggcgcag cgatcctgct ggctatcttc agcctgtggc agatgcctca
    16861 ctcctatgcc atcgccattt tccgctttaa ggattatcag gcggcaaaca ttccggtatt
    16921 gccagtggta aaaggcattt cggtggcgaa gaatcacatc acgctgtata tcatcgcctt
    16981 tgccgttgcc acgctgatgc tctctcttgg cggttacgct gggtataaat atctggtggt
    17041 cgccgcggcg gtgagcgtct ggtggttagg tatggccctg cgcggttata aagttgctga
    17101 tgacaggatc tgggcgcgca agctgttcgg cttctctatc atcgccatca cagccctctc
    17161 ggtgatgatg tccgttgatt ttatggtgcc ggactcgcat acgctgctgg ctgctgtttg
    17221 gtaacacaac ctctctatta aaaaggtgct acggcacctt ttttattgtc tgagatcaga
    17281 gatacggcta ataaccgtat gtcttgacag agtgagcaga tgaacataaa ttaatcatac
    17341 ggttgatagc cgtattatgt atgagatcga ctccctacga tttcattcag ttaatcaaag
    17401 aggtcataat gcctggaaaa acagccactc tacccgatgt cgataaaacg ctgaaaaatg
    17461 cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa acttcgtgaa gctgcgcaag
    17521 ccgttggggt agatttaagc gcttttattt tgagcgctgc tatggaacgc gccgaaagtg
    17581 tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag ctgggaactg atgaaccaac
    17641 tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc gttaatgaaa aggaaaaaca
    17701 gcgatggtcg acaagcatga agagattact ctgcccatag tcctctcctg taattatcag
    17761 tctgatatta cttatcctgg gcaaaaacag tttgattgcg gtaaccctgt tatcgataaa
    17821 tttgtacgcg catcgctaaa gaaaagtgtg cgtaatagcg actgtgcggc taaagcactt
    17881 attgacagac aaagtggtga actgatcggc atctgtactt ttacggcata ttcgctggaa
    17941 aaacaacgcg tttctggcgt ccttcagggt tcacaacctt cagaaattgg tgttgtcaga
    18001 ttagtcatgt tgggggtagc acggaagtat caaaagcggg gctttggtca ggacctacta
    18061 tgtgattttt ttgaacatgt aaaaataatt caccaggcat taccaattaa aggggtttat
    18121 cttgatgctg accctgccgc cattaatttt tatgctcgtc tcggctttgt tcagctttca
    18181 gcgacaccaa atgcttttgg tgctgtacct atgtttttgg cgattcagca tattctcgcg
    18241 gcttagatta ttatcgccac ccgccagggc aagttttgcc ttagccgggg gctgagcagc
    18301 gttaccactt ttgttcaatt gcccccgctt tcagcaactc cacatccttg ccgttaatgg
    18361 ttgccgttgc ggcaacgatt gtggtcctta cagttgcaca agattttcgc tcaaattccg
    18421 gtttataaag aataagagac gaattactgg taaatgaacc aaataggtaa tcagtaaagc
    18481 taatattcag ccggtttaaa ccgaaaggcg cgaagccaac cgtttggtta atctccttac
    18541 cggatgggcg acgaatgctg aatgtcacgc gaagattggc cacttcgcct aatttattgg
    18601 tggtggcgaa gaggctatcc atgcaggaaa aacctttatc taaaaaatta tctcgcatcc
    18661 cagaacgggt aaccgtaatc acgggtaacg tatcgtcaac cgtttttctg tcaatcatgt
    18721 cttttcgaca agtagccaga aagtcttccg ggccaccata aatgtggaca cctttatcaa
    18781 gatattcctt cgctttctta caatctacct caacatactg accattggcg tacataatgc
    18841 cca
//
LOCUS       DAZPJR010000071        18523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPJR010000071 DAZPJR010000000
VERSION     DAZPJR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.072"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHVB7_002338"
     CDS             <1..1710
                     /locus_tag="ACHVB7_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR1914309.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHVB7_002339"
     CDS             1711..3360
                     /locus_tag="ACHVB7_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR1914310.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHVB7_002340"
     CDS             3365..4141
                     /locus_tag="ACHVB7_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR1914311.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHVB7_002341"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHVB7_002341"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR1914312.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHVB7_002342"
     CDS             complement(5430..6752)
                     /locus_tag="ACHVB7_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR1914313.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHVB7_002343"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHVB7_002343"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR1914314.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHVB7_002344"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHVB7_002344"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR1914315.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHVB7_002345"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHVB7_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR1914316.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="ACHVB7_002346"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="ACHVB7_002346"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR1914317.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="ACHVB7_002347"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="ACHVB7_002347"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR1914318.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="ACHVB7_002348"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="ACHVB7_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR1914319.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="ACHVB7_002349"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="ACHVB7_002349"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR1914320.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4535 c         4867 g         4595 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAZPJR010000072        18513 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPJR010000072 DAZPJR010000000
VERSION     DAZPJR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.073"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="ACHVB7_002350"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="ACHVB7_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR1914321.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="ACHVB7_002351"
     CDS             735..1040
                     /locus_tag="ACHVB7_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914322.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHVB7_002352"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHVB7_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR1914323.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="ACHVB7_002353"
     CDS             1788..2534
                     /locus_tag="ACHVB7_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1914324.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="ACHVB7_002354"
     CDS             2534..4630
                     /locus_tag="ACHVB7_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1914325.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="ACHVB7_002355"
     CDS             4676..5815
                     /locus_tag="ACHVB7_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR1914326.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="ACHVB7_002356"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="ACHVB7_002356"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR1914327.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="ACHVB7_002357"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="ACHVB7_002357"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR1914328.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHVB7_002358"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHVB7_002358"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR1914329.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHVB7_002359"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHVB7_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR1914330.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHVB7_002360"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHVB7_002360"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1914331.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHVB7_002361"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHVB7_002361"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR1914332.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="ACHVB7_002362"
     CDS             complement(12873..13730)
                     /locus_tag="ACHVB7_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR1914333.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHVB7_002363"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHVB7_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR1914334.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHVB7_002364"
     CDS             complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHVB7_002364"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR1914335.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHVB7_002365"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHVB7_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHVB7_002366"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHVB7_002366"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR1914336.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHVB7_002367"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHVB7_002367"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR1914337.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4544 a         4912 c         4782 g         4275 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataata
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc tgttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctgccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaattc cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gctgccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggtg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaa
//
LOCUS       DAZPJR010000073        18214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPJR010000073 DAZPJR010000000
VERSION     DAZPJR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.074"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHVB7_002368"
     CDS             15..1091
                     /locus_tag="ACHVB7_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1914338.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHVB7_002369"
     CDS             complement(1484..1825)
                     /locus_tag="ACHVB7_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR1914339.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHVB7_002370"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHVB7_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR1914340.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHVB7_002371"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHVB7_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR1914341.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHVB7_002372"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHVB7_002372"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR1914342.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHVB7_002373"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHVB7_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR1914343.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHVB7_002374"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHVB7_002374"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR1914344.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHVB7_002375"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHVB7_002375"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR1914345.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHVB7_002376"
     CDS             complement(7167..7826)
                     /locus_tag="ACHVB7_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR1914346.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHVB7_002377"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHVB7_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR1914347.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHVB7_002378"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHVB7_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR1914348.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHVB7_002379"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHVB7_002379"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR1914349.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHVB7_002380"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHVB7_002380"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR1914350.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHVB7_002381"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHVB7_002381"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR1914351.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHVB7_002382"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHVB7_002382"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR1914352.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHVB7_002383"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHVB7_002383"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR1914353.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHVB7_002384"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHVB7_002384"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR1914354.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="ACHVB7_002385"
     CDS             complement(17270..18112)
                     /locus_tag="ACHVB7_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR1914355.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4671 a         4575 c         4392 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaat
//
LOCUS       DAZPJR010000074        18072 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPJR010000074 DAZPJR010000000
VERSION     DAZPJR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.075"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            345..1023
                     /locus_tag="ACHVB7_002386"
                     /pseudo
     CDS             345..1023
                     /locus_tag="ACHVB7_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1067..5917
                     /locus_tag="ACHVB7_002387"
     CDS             1067..5917
                     /locus_tag="ACHVB7_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR1914356.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6285..7940
                     /gene="lldP"
                     /locus_tag="ACHVB7_002388"
     CDS             6285..7940
                     /gene="lldP"
                     /locus_tag="ACHVB7_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR1914357.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7940..8716
                     /gene="lldR"
                     /locus_tag="ACHVB7_002389"
     CDS             7940..8716
                     /gene="lldR"
                     /locus_tag="ACHVB7_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR1914358.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8713..9903
                     /gene="lldD"
                     /locus_tag="ACHVB7_002390"
     CDS             8713..9903
                     /gene="lldD"
                     /locus_tag="ACHVB7_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR1914359.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10099..10572
                     /gene="trmL"
                     /locus_tag="ACHVB7_002391"
     CDS             10099..10572
                     /gene="trmL"
                     /locus_tag="ACHVB7_002391"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR1914360.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10625..11446)
                     /gene="cysE"
                     /locus_tag="ACHVB7_002392"
     CDS             complement(10625..11446)
                     /gene="cysE"
                     /locus_tag="ACHVB7_002392"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR1914361.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11526..12545)
                     /gene="gpsA"
                     /locus_tag="ACHVB7_002393"
     CDS             complement(11526..12545)
                     /gene="gpsA"
                     /locus_tag="ACHVB7_002393"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR1914362.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12545..13012)
                     /gene="secB"
                     /locus_tag="ACHVB7_002394"
     CDS             complement(12545..13012)
                     /gene="secB"
                     /locus_tag="ACHVB7_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR1914363.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(13075..13326)
                     /gene="grxC"
                     /locus_tag="ACHVB7_002395"
     CDS             complement(13075..13326)
                     /gene="grxC"
                     /locus_tag="ACHVB7_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR1914364.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13468..13899)
                     /locus_tag="ACHVB7_002396"
     CDS             complement(13468..13899)
                     /locus_tag="ACHVB7_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR1914365.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14144..15688
                     /gene="gpmM"
                     /locus_tag="ACHVB7_002397"
                     /gene_synonym="pgmI"
     CDS             14144..15688
                     /gene="gpmM"
                     /locus_tag="ACHVB7_002397"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR1914366.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15722..16981
                     /gene="envC"
                     /locus_tag="ACHVB7_002398"
     CDS             15722..16981
                     /gene="envC"
                     /locus_tag="ACHVB7_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR1914367.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16985..17944
                     /locus_tag="ACHVB7_002399"
     CDS             16985..17944
                     /locus_tag="ACHVB7_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR1914368.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17931..18072)
                     /locus_tag="ACHVB7_002400"
                     /pseudo
     CDS             complement(17931..18072)
                     /locus_tag="ACHVB7_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4554 a         4657 c         4691 g         4170 t
ORIGIN      
        1 catttattaa ttaacaagca gttacgtctc tttttttcgg atttaattgt gaaattttaa
       61 tcatcatatt catacaataa attatctgta aattttttcc cagacatggt ttctttcata
      121 tttgacattt attaataatc atcattagaa tccacgaaaa taatatattc atacaaacac
      181 agacatcttt tgaaacgaat gatacatttt tatgatgaat gcttaaaaca atatttaatc
      241 gacacacatc actgtcatta atgtaaagca acatagatca ccaataatac aatggacaac
      301 tctaccggca ttgatcgctg gtcggaggaa ataaggattt ttacatgaac ttgaagaaga
      361 tttttttcag tgctgtcact gtttcagttc tctgcgcatt aacgggatgt gactacattg
      421 aagaaggcaa gccagaaagt agtttattga aacaacaaga agaacattaa aattgatcta
      481 cttgaaaaac aacaagcaca gctaaagagt caactggaaa ctattcaaaa acaacaaact
      541 ggcataataa gcagtacaaa gactttaact cacgtaatta aatccgttaa agatcaacaa
      601 aacactttta tttttaccga gtttaaccca gcaaaaacga agtatttcat cctgaacaat
      661 ggttcggtgg ctttagcggg tcgagtgtta tccattgacg ccacagagaa tggcagtgtt
      721 attcatattt cactggtcaa cttattaagt acacctatct caaatattgg ttttaatgcg
      781 acatggggtg gtgaaaaacc tgtcgatgcc aaagagtttg cccggtggca acaattactt
      841 ttcaacacat caatgaaatc cacattgaaa ttattaccag gtcaatggca agacattaat
      901 ttgaccctga agggtgtatc gcccaataac ctgggatatc tgaagttagc catcaacatg
      961 gaaaatattc agttcgacaa tcttccctct gctgaaaatc ggcagaaaag aagcaaaaaa
     1021 taaatgcagt tagtacgctt ataacaagga atagcgagca tcattaatga acaaaatatt
     1081 taaagttatc tggaaccctg cgacagggaa ttatactgtt accagcgaaa cggcaaaaag
     1141 ccgtggcaag aaatctgggc gcagtaagct gttaatttct gcgctggttg cgggtggaat
     1201 gttgtcgtcg tttggggcat tggcgaatgc cgggaatgac aacggccagg gtgttgatta
     1261 cggtagtgga tcagctggcg acagctgggt tgctataggc aaaggggcga aagcaaatac
     1321 ttttatgaac accagtggtt ccagtactgc tgtgggttat gacgctatag ctgaaggcca
     1381 atatagctct gccatcgggt caaaaaccca tgcgattggt ggtgcatcaa tggcctttgg
     1441 ggttagtgca atatcagaag gcgatagaag tatagcactg ggtgcctctt cgtattcatt
     1501 gggccaatac tcaatggccc tcggccgtta ttcaaaagca ttgggtaaat tgtctattgc
     1561 tatgggggac tcttccaaag cggaaggagc aaacgccatt gccctgggaa atgccactaa
     1621 agctactgag attatgagta ttgctcttgg cgacaccgcc aatgcgtcaa aagcgtattc
     1681 aatggcgctg ggagcaagta gcgtcgcatc tgaagaaaac gcaattgccc tggggcgtag
     1741 cagtgtagct agcggtactg acagcctcgc atttggcaga caatcacttg ccagcgcagc
     1801 gaacgctatt gcgataggtg ctgagaccga agccgctgaa aatgcaactg ctattggcaa
     1861 taatgcgaag gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa
     1921 agtcaatact atcgcattag gaaatggcag ccaggctctg gcagataatg caatcgccat
     1981 aggccagggc aacaaagctg atggcgtgga tgccatcgct ctgggtaatg gtagccagtc
     2041 gagaggctta aacaccattg ccttaggcac agccagtaat gcaactggtg ataagagtct
     2101 tgcgcttggt agtaatagca gtgccaacgg tattaactct gtcgcgctgg gcgcagattc
     2161 cattgcggat ttagacaata ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt
     2221 taatgtgaaa aatggcgcga tcaagtctga cagttacgat gccattaatg gttcacagct
     2281 ttatgccatt agcgactcgg tagcaaaaag gcttggagga ggggctgcag tagatgttga
     2341 tgacggtact gttacagcac caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg
     2401 ggctgcgctc gctgtacttg atgaaaacac cctgcaatgg gaccaaacca aaggcaaata
     2461 cagcgctgct catggtacta gtagcccaac tgccagcgta atcaccgatg ttgcggatgg
     2521 cacgatttca gcctccagta aggatgcggt taacggttcc caactgaaag ctaccaatga
     2581 cgatgtcgaa gccaacaccg ccaatatcgc tactaatacc agcaacattg ccacgaatac
     2641 agcaagtatt gccaccaata ccaccaatat caccaacctg acggattccg ttggtgacct
     2701 tcaggctgat gccctgctct ggaacgaaac taaaaaggca ttcagtgcag ctcacggcca
     2761 ggataccacc agcaaaatca ccaacgttaa agatgccgac ctgacggctg acagcactga
     2821 tgctgttaac ggctctcagc tgaaaaccac caacgatgct gtggcgacga ataccaccaa
     2881 tatcgccaat aacacttcca atattgccac taacaccacc aacatctcta atctgactga
     2941 gacggtgact aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtattcac
     3001 ggcagctcat ggcaccgaga ccaccagcaa aatcaccaac gttaaagatg gcgacctgac
     3061 gactggcagc accgatgccg ttaacggctc tcagctgaaa accaccaacg atgccgtggc
     3121 gacgaatacc accaatatcg ccactaacac caccaacatc tctaatctga ctgagacggt
     3181 gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtct tcactgcagc
     3241 tcatggcaac aataccgcca gcaaaatcac caatatcctg gacggcacag tcactgcaac
     3301 cagttccgat gccattaacg gtagccagct ttatgactta agcagcaata tcgccaccta
     3361 cttcggcggc aatgcttctg tgaatactga cggtgtgttt accggtccaa cctacaaaat
     3421 cggtgaaaca aattattata acgtcggcga tgcactggct gcgattaact cctcatttag
     3481 cacgtctctc ggcgatgctc tgctttggga tgccaccgca ggtaaattca gtgccaaaca
     3541 cggtactaat ggtgacgcaa gcgtgatcac tgatgtcgca gatggtgaaa tttcagactc
     3601 cagttctgac gcagtaaacg gctcacaact ccacggcgtg agcagttatg ttgttgatgc
     3661 gctggggggt ggtgccgaag tcaatgcaga cggcaccatc actgcgccga cgtacaccat
     3721 tgctaatgct gattacgata atgtcggtga tgccctgaat gctatcgata ccactcttga
     3781 cgacgctctg ctctgggatg cggacgccgg tgaaaatggt gcatttagcg ccgctcacgg
     3841 aaaagataaa actgccagtg taatcactaa cgtcgctaac ggtgcaatct ctgctgccag
     3901 cagcgacgcg attaacggct cacaactcta taccaccaat aagtacatcg ctgatgcgct
     3961 gggtggtgac gcagaagtca acgctgacgg caccatcacc gcaccgactt acaccattgc
     4021 gaacgccgag tacaacaacg tcggtgacgc cctggatgcg cttgatgata acgccctgct
     4081 gtgggatgag actgccaatg gcggtgctgg agcctacaat gccagccatg acggtaaagc
     4141 cagcatcatc actaatgtcg ctaatggcag tattagtgag gacagtaccg atgcagtgaa
     4201 cggttctcag ttgaatgcga cgaatatgat gattgagcag aacacccaaa ttatcaatca
     4261 gctcgctggt aacaccgacg caacctatat cgaagaaaat ggcgctggta tcaactacgt
     4321 tcgtactaac gacaacgatt tagcctttaa cgatgcaagc gcctctggtg tcggcgctac
     4381 agctgtaggt tataacgctg tcgcgtctgg tgccagcagc gtagccattg gtcagaacag
     4441 cagcagcacc gttgataccg gtattgcgct gggtagcagc tccgtttcca gccgtgtgat
     4501 tgccaaaggt tctcgtgaca ctagcgtaac ggaaaatggc gtggtgattg gttatgacac
     4561 cactgacggc gaactgctag gtgcattgtc aattggtgat gacggtaaat accgccaaat
     4621 catcaacgta gctgatggtt cagaagccca tgacgccgtt acggttcgcc agttgcagaa
     4681 cgctattgga gcggtcgcca ctacgccaac caagtacttt cacgccaact caacggcaga
     4741 agactcactg gccgttggtg aagactcgct ggcaatgggt gcgaaaactg tcgttaatgg
     4801 taatgcaggt attggtatcg gtttgaacac tctggttcta gctgatgcga tcaacggcat
     4861 tgctatcggc agtaacgcac gcgcaaacca tgcaaacagt atcgcaatgg gtaacggttc
     4921 tcagaccacc cgtggtgcac agactggcta caccgcctac aacatggacg caccgcagaa
     4981 ctctgtaggt gagttttctg tcggtagtga agacggtcaa cgtcagatca caaacgtcgc
     5041 agctggttca gcggataccg atgcggttaa cgtgggtcag ttgaaagtca ctgatgagcg
     5101 cgtagcgcaa aatacccaga gcattactaa cctgaacaat caggtcacta atctggatac
     5161 tcgcgttact aatatcgaaa acggtattgg cgacattgtc accaccggta gcaccaagta
     5221 cttcaagacc aacaccgatg gcgtagatgc caacgcccag ggtaaagata gcgttgctat
     5281 tggttctggt tccattgctg ccgctgacaa cagcgtcgca ctgggtaccg gttccgttgc
     5341 agaggaagaa aatacaatct ctgtaggttc ttctactaac caacgccgta tcaccaacgt
     5401 agctgccggt gttaatgcca ccgatgcggt taacgtgtcg cagctgaaat cttctgaagc
     5461 aggcggcgtg cgctacgaca ccaaagctga tggttctatc gactatagca atatcaccct
     5521 cggtggtggc aacggcagta cgactcgtat cagcaacgtc tccgctggcg tcaacaacaa
     5581 cgacgcggtg aactacgcgc agttgaagca aagcgtgcag gaaacgaagc aatacaccga
     5641 tcagcggatg gttgagatgg ataacaaact gtctaaaact gaaagcaagt tgagcggtgg
     5701 tatcgcttct gcaatggcaa tgaccggtct gccgcaggct tatacaccgg gtgccagcat
     5761 ggcttctatt ggtggcggta cttacaacgg tgaatcggca gttgctttag gtgtatcgat
     5821 ggtgagcgcc aatggtcgtt gggtctacaa attacaaggt agtaccaata gccagggtga
     5881 atactccgcc gcactcggtg ccggtattca gtggtaatga tccattaagt tagtgtgact
     5941 aagggcaaac gccaaattgc ctgatgcgtt acgcgcatca ggcaagataa ggcgccccgg
     6001 gtcaacaatc taagggcaat tctctgatga ggattgccct tttctttacc agacatctcc
     6061 ccccacaaga attggcccta ccaattcttc gcttatctga cctctggttc acaattcccc
     6121 aattaaaact cacatcaata ttgccaatac ataacattta gttaaccatt cattgtcatt
     6181 atccctacac aacacaattg gcagtgccac ttttacacaa cgtgtgacaa ggagatgagc
     6241 aacagactca ttacacgatg tgcgtggact ccaggagacc tgcaatgaat ctctggcaac
     6301 aaaactacga tcccgccggg aatatctggc tttccagtct gatagcatcg cttcccatcc
     6361 tgtttttctt ctttgcgctg attaagctca aactgaaagg atacgtcgcc gcctcatgga
     6421 cggtagcaat cgcccttgcc gtggctttgc tgttctataa aatgccggtc gctaacgcgc
     6481 tggcctcggt ggtttatggc ttcttctatg gtttgtggcc catcgcgtgg atcattattg
     6541 cagcagtgtt cgtctataag atctcggtga aaaccgggca gtttgacatc attcgctcgt
     6601 ctattctttc gataacccct gaccagcgtc tgcaaatgct gatcgtcggt ttctgtttcg
     6661 gcgctttcct tgaaggagcc gcaggctttg gcgcaccggt agcaattacc gccgcattgc
     6721 tggtcggcct gggttttaaa ccgctgtacg ccgccggact gtgcctgatt gttaacaccg
     6781 cgccagtggc atttggcgcg atgggcattc caatcctggt tgccggacaa gtaacaggca
     6841 tcgacagctt tgagattggc cagatggtgg ggcggcagct accgtttatg accattatcg
     6901 tgctgttctg gatcatggcg attatggacg gctggcgcgg tatcaaggag acgtggcctg
     6961 cggtcgtggt tgcgggcggc tcgtttgcca tcgctcagta ccttagctct aacttcattg
     7021 ggccggagct gccggacatt atctcttcgc tggtatcact gctctgcctg acgctgttcc
     7081 tcaaacgctg gcagccagtg cgcgtattcc gttttggtga tttgggggcg tcaaagattg
     7141 atatgacgct ggcccacacc ggttacactg cgggtcaggt gttacgtgcc tggacaccgt
     7201 tcctgttcct gacagctacc gtaacactgt ggagcatccc gccgtttaaa gccctgttcg
     7261 catcgggtgg tgcgctgtat gagtgggtga tcaacattcc ggtgccgtac ctcgataaac
     7321 tggttgcccg tatgccacca gtggtcagcg aggctacagc ctatgccgcc gtgtttaagt
     7381 ttgactggtt ctctgccacc ggcaccgcca ttctgtttgc tgcactgctc tcgattgtct
     7441 ggctgaagat gaaaccgtct gacgctatca gcaccttcgg cagcacgctg aaagaactgg
     7501 ctctacccat ctactccatc ggcatggtgc tggcgttcgc ctttatctcg aactattccg
     7561 gactgtcatc aacactggca ctggcactgg cgcacaccgg tcatgcattc accttcttct
     7621 cgccgttcct cggctggctg ggggtattcc tgaccgggtc ggatacctca tctaacgccc
     7681 tgttcgccgc gctgcaagcc accgcagcac aacaaattgg cgtctctgat ctgttgctgg
     7741 ttgccgccaa taccaccggt ggcgtcaccg gtaagatgat ctccccgcaa tctatcgcta
     7801 tcgcctgtgc ggcggtaggc ctggtgggca aagagtctga tttgttccgc tttactgtca
     7861 aacacagcct gatcttcacc tgtatggtgg gcgtgatcac cacgcttcag gcttatgtct
     7921 taacgtggat gattccttaa tgattgtttt acccagacgc ctgtcagacg aggttgccga
     7981 tcgtgtgcgg gcgctgattg atgaaaaaaa cctggaagcg ggcatgaagt tgcccgctga
     8041 gcgccaactg gcgatgcaac tcggcgtatc acgtaattca ctgcgcgagg cgctggcaaa
     8101 actggtgagc gaaggcgtgc tgctcagtcg acgcggcggc gggacgttta ttcgctggcg
     8161 tcatgacaca tggtcggagc aaaacatcgt ccagccgcta aaaacgctga tggccgatga
     8221 tccggattac agtttcgata ttctggaagc ccgctacgcc attgaagcca gcaccgcatg
     8281 gcatgcggca atgcgcgcca cacctggcga caaagaaaag attcagcttt gctttgaagc
     8341 aacgctaagt gaagacccgg atatcgcctc acaagcggac gttcgttttc atctggcgat
     8401 tgccgaagcc tcacataaca tcgtgctgct gcaaaccatg cgcggtttct tcgatgtcct
     8461 gcaatcctca gtgaagcata gccgtcagcg gatgtatctg gtgccaccgg ttttttcaca
     8521 actgaccgaa caacatcagg ctgtcattga cgccattttt gctggtgatg ccgacggggc
     8581 gcgtaaagca atgatggcgc accttagttt tgttcacacc accatgaaac gattcgatga
     8641 agatcaggct cgccacgcac ggattacccg cctgcccggt gagcataatg agcattcgag
     8701 ggagaaaaac gcatgattat ttccgcagcc agcgattatc gcgccgcagc gcaacgcatt
     8761 ctgccgccgt tcctgttcca ctatatggat ggtggtgcat attctgaata cacgctgcgc
     8821 cgcaacgtgg aagatttgtc agaagtggca ctgcgccagc gtattctgaa aaacatgtcc
     8881 gacttaagcc tggaaacgac gctgtttaat gagaaattgt cgatgccggt agcactggca
     8941 ccggtgggtt tgtgtggcat gtatgcgcgt cgtggcgaag ttcaggcagc caaagcggcg
     9001 gacgcgcatg gcattccgtt tactctctcg acggtttccg tttgcccgat tgaagaagtc
     9061 gcgccagcca tcaagcgccc aatgtggttc cagctttatg tactgcgcga tcgcggcttt
     9121 atgcgtaacg cgctggagcg agcaaaagca gcgggttgtt cgacgctggt tttcaccgtg
     9181 gatatgccga caccgggcgc acgctaccgt gatgcgcatt caggtatgag cggcccaaat
     9241 gcggcaatgc gccgctactt gcaggcggtg acgcatccgc aatgggcgtg ggatgtgggc
     9301 ctgaacggtc gtccacatga tttaggtaat atctcagctt atctcggcaa accgaccgga
     9361 ctggaagatt acatcggctg gctggggaat aacttcgatc cgtccatctc atggaaagac
     9421 cttgagtgga tccgcgattt ctgggatggc ccgatggtga tcaaagggat cctcgatccg
     9481 gaagatgcgc gcgatgcagt acgttttggt gctgatggaa ttgtggtttc taaccacggt
     9541 ggccgccagc tggacggtgt actctcttcc gctcgtgcgt tgcccgctat tgcagatgcg
     9601 gtgaaaggtg atatcgccat tctggcggat agcggaattc gtaacgggct tgatgtcgtg
     9661 cgtatgattg cgctcggtgc cgacaccgta ctgctgggtc gtgctttcct gtatgcgctg
     9721 gcaacagcgg gccaggcggg tgtagctaac ctgctaaatc tgatcgaaaa agagatgaaa
     9781 gtggcgatga cgctgactgg cgcgaaatcg atcagtgaaa ttacgcaaga ttcgctggtg
     9841 caggggctgg gtaaagagtt gcctacggca ctggctccga tggcgaaagg gaatgcggca
     9901 tagtcgtttg cgccccctca ccctaaccct ctccctcagg gagaggggac cgttcggcgc
     9961 tgtatgtact ccctcactct gaaacgacac cgcactcttt ttttctccct cgcccctccg
    10021 gggagggccg gggtgagggg aaaaggccgc acggttcacc aattttctgc tatcctgccc
    10081 ccgcactaag ggggcagtat gctaaacatc gtactttacg aaccagaaat tccgccaaat
    10141 actggcaaca tcatccgtct ttgcgctaat accggctttc gtctgcatat catcgaaccg
    10201 atgggatttg cctgggacga taagcgcctg cgccgcgcgg ggctggacta tcacgagttt
    10261 accgccgtta cgcgtcatca tgactatcgc gcgttcctcg aagcagaaaa tccccagcgc
    10321 ctgtttgccc tcaccacgaa aggtacgcct gctcacagcg ccgtaagcta tcaggatggc
    10381 gattatctga tgttcggccc ggaaacacgc ggcctgccag cgagcattct tgatgccctg
    10441 cccgctgaac aaaaaattcg cattccgatg gtgccggaca gccgcagcat gaatctgtcc
    10501 aatgcggtgt cggtagtggt gtatgaagcc tggcggcagc tgggatatcc gggagcgttg
    10561 ttgagagatt agttactgta tgccggatag atgattacat cgcatccggc acgatcacag
    10621 gacattagat cccatcccca tactcaaatg tatggttaat accgttgaaa tgctggtcca
    10681 tatccattga tggcttatcg ctgtctggtt taccgacaat acgagccgga acgccagcgg
    10741 cggttgtatg cggcggcacc ggttgcagca ccacggaacc tgcgccaatc ttcgcgccgc
    10801 gaccaacttc aatattgccg aggattttcg cgcccgcgcc aatcatcaca ccttcacgaa
    10861 ttttcgggtg acggtcacca ccagatttac ccgtaccgcc aagcgtcaca gattgcagaa
    10921 tcgatacgtc gttttcaatt accgccgttt cacccacaac gatgcccgtg gcgtggtcga
    10981 gcataatgcc acggccaatt tttgctgccg ggtgaatatc gacctggaac gtcacagaaa
    11041 cctggttttg cagaaagatt gccaatgcgc gacgcccctg attccacaac cagtgaccga
    11101 tgcgataggc ctgcaaggca tgaaaaccct tcaggtataa caacggggtt gagtatttat
    11161 cgactgccgg gtcgcgggta cgcaccgcct gaatatcaca ggccgcagag gcgatcattt
    11221 ccgggtcagc ggcgtaggct tcttccacca cttcacggat agcaatagca ggcataattg
    11281 gcgatgacag cttgttcgcc agcatgtagc tcagtgcact accaaggttt tcgtgcttga
    11341 gtagcgtcgc gtggtaaaaa ctggccagca ttggctcaca gtccgccagc gttctggctt
    11401 cggctttaat attgttccag acaatttcca gttcttcaca cgacattgct tactccacac
    11461 gatgagataa tgaccggccc gttctgcgcg ggccgggtca tagcggtaac aaaggttccc
    11521 tggggttagt ggctgctgcg ctcgtccttg cgtgcacgac ctaataacgt caatgctgcc
    11581 tcgcgcgcgt tttttccgca atataatact tgataaattt cctcggttat tggcatttct
    11641 acgccgaagc gatgcgccag ttcgcggact tctttcgtat tgcggtagcc ttccaccacc
    11701 tgaccaatct tctcctgcgc gctttgtaca tccatgccct gaccgagcat catgccaaaa
    11761 cggcggttac gcgactggtt gtcggtacag gtaagcacca gatcgcccag ccccgccatg
    11821 cccataaagg tggcaggatc ggcacccagc gccgcgccaa gacgcgacat ttcagccagc
    11881 ccacgggtga tcagtgccgt acgcgcattc gcaccaaaac cgataccgtc ggacatcccc
    11941 gcgccaatgg caatgacgtt tttcaccgca ccgccaagct gcacaccgat gaaatccggg
    12001 ttgctgtaaa cgcggaaact tttgccacag tgcagcaatt gttggagatc atcggcaaag
    12061 gtctggtcgg tagaggccaa cgaaatagcc gtcggtaaac ctgccgccag ttctttcgca
    12121 aacgttgggc cagagataac cgccagcgga atttgatcgc ctaaggcctc acgcgccacg
    12181 tcctgtaaca gacgtccggt ttccgcttcc agccctttgg tcgcccacac cagacgcgca
    12241 tcaggacgca tcagcggttt aatctggcgc agcacttcac caaagacatg gctgggtacg
    12301 acgacgagaa tattacggct ggctgccagc gcagtggcga gatcgctttc aagatggagc
    12361 gtatcgggaa aaggcacatc ggggagaaac gcggcgttac agcggtcgcg ttcaagcgtt
    12421 gcgatatgtt cagggtcatg gccccagagg acaacctcgt ggccatttct tgccagggta
    12481 atggcaagag cggtgccgta cgagccggca ccgatcacag tcattgaagc attacgttgg
    12541 ttcatcaggc atcctgatgt tcttcagtac cttcgccagc ctgctgctgc aaatagttca
    12601 tgaacagcgc atcgaagtta atcggcgcaa ggttcagttg cgggaatgta ccgcgggata
    12661 ccacgctggt gatgcactca cgagcatacg ggaacagaat gttcgggcag tatgcgccca
    12721 ggcaatgtgc catctgggtg ccttcgatac ccgcgatgga gaaaataccg ccctgctgaa
    12781 cttcacacag gaacgcggtt tcttcgccca gggaggccgt tacggtaaca cgcagtacca
    12841 cttcgtatac gtcatctgcc agttgggaag atgccgtatc cagatcaagt ttaacttctg
    12901 gttgccaatc tttctggaaa acgtgcggcg cgttcggcgc ttcgaaagag atatccttgg
    12961 tataaatacg ttggatctgg aaagtcattt cagtgttgtt ttgttctgac atgtgtagaa
    13021 aacccttaag tgttgtcctt aaatactgcg caatgtaatg ccaacgttca cacgttattt
    13081 cagcagggga tccagtccac cacgtgcatc caatgcatac aagtcatcac agccgccaat
    13141 gtgctgtgcg tcaataaaaa tctggggaac cgtggtgcga ccgctgcgtt tgatcatctc
    13201 ttcacgcttg gcggcattgc catcgatcgg caactcctga aaactcacgc ccttgctgct
    13261 cagcagtgct tttgcacgat ggcaatacgg gcaggtttct ttggtataga tttcaacatt
    13321 ggccataatt tagctcctgt ttttttaccc tgaaatttca tgttgcaggg cggctgcaaa
    13381 atggagtgtc cccagaagct tataacaata agtcgctggg ggcctaattg cagctaacgg
    13441 cctgcatcat gaaagacgac aggtaaatta tttgccgcgc accagaggca agttttcgcc
    13501 agcccagcca gcaacgccct ctttcagcac gaatacttgc gcaaaaccag ctttcgtcag
    13561 cgcgtttgca ggctcctggc actgcatgcc agaaccgtct accacgataa ccggtttgtc
    13621 tttgtgcttc tcaagctcac caacattgtt ggctttgatt tcgctcggca acagattgat
    13681 agaacctgcg atatggcctt tacggaagtc atcacgctga cgtaaatcca caaccacagc
    13741 gtcttctttg ttgatcagac gcgtagcttc accacgagta atcaccttca ctttcgaggt
    13801 caggctctta aacgtagtca caagaaccgc caccagtaac gcgatccagg cgatactcag
    13861 tatgggatga cggccaacaa attgcataat ttcttgcatg gggggtaaca actcccgacg
    13921 tagtgattaa aaaaaccagg aaaggagtat acctgcgcgg tgcggcaaat acagccagcg
    13981 cgttaactgg aatgcaattt tgcggggcac gacgaaaaaa aagcgcaaat ctggcatcgg
    14041 cttgccgcga gcggtcgtat attttgatct tcagaggcta ttttatcgat tcagctgtag
    14101 taaaattacg caaattttga ctcttgagta tgaggttgtc gcaatgtcgg tttctaaaaa
    14161 acctatggta ctggtgattc tggatggata tggctatcgt gaagaacagc aggataacgc
    14221 catttttagt gctaaaaccc cggtaatgga tgcactgtgg gccaatcgtc cgcataccct
    14281 gatcgacgct tccggtctgg aagtcggtct gcctgaccgt cagatgggta actccgaagt
    14341 aggccacgtt aacctgggtg ccggccgcat cgtgtatcag gacctgactc gtctggacgt
    14401 tgaaatcaaa gatcgcgctt tctttgctaa tccagtgctg actggcgcgg tagataaagc
    14461 gaaaaacgca ggtaaagcag tacacattat gggtctgctc tccgcaggcg gcgtacacag
    14521 ccacgaagat cacatcatgg cgatggtaga actggcagct gaacgcggcg cagaaaaaat
    14581 ctacctgcac gcattccttg acggtcgcga caccccgccg cgcagtgctg aatcctcgct
    14641 gaaaaaattt gaagagaaat ttgctgcgct gggtaaaggc cgcgtagcgt ccatcattgg
    14701 tcgttactac gcgatggacc gcgataaccg ttgggatcgc gttgaaaaag cctatgacct
    14761 gctgactctg gcgcagggtg agtttcaggc cgatactgcc gttgctggtt tgcaggcagc
    14821 ttatgctcgc gacgaaaacg acgagttcgt gaaagcgacc gttattcgtg cagaaggtca
    14881 gccagatgcg gcaatggaag acggcgatgc gctgattttc atgaacttcc gtgctgaccg
    14941 cgcgcgtgaa atcactcgtg cattcgtgaa cgctgatttc gatggcttcg cgcgtaagaa
    15001 agtggttaac gtcgatttcg tgatgctgac cgaatacgcc gctgacatca aaactgcggt
    15061 tgcttaccca cccgcttccc tggttaacac cttcggcgag tggatggcga aaaacgacaa
    15121 aactcagttg cgtatttccg aaaccgaaaa atatgcccac gttactttct tcttcaacgg
    15181 tggcgtagaa gagtcgttca aaggcgaaga tcgcattctg atcaactcac cgaaagtggc
    15241 tacctacgat ctgcaaccgg aaatgagctc cgcagagctg accgaaaaac tggttgcggc
    15301 catcaagagc ggcaaatacg acaccattat ctgtaactat ccgaacggcg acatggtagg
    15361 tcacaccggg gtgatggaag cggcggttaa agcggttgaa gcactggatc actgcgtgga
    15421 agaagtcgcg aaagcggttg agtccgtggg tggacaactg ctgatcaccg ctgaccacgg
    15481 taacgctgaa cagatgcgcg atccggcaac gggtcaggca cacacggcac acaccaacct
    15541 gccagttccg ctgatttacg ttggtgataa gaacgtgaaa gcggttgcag gcggcaaact
    15601 ttctgacatc gcgccgacca tgttgtcgct gatgggtatg gaaatcccgc aagagatgac
    15661 tggtaagccg ctgttcatcg tggaataatc cctccccatg aggggaaagg cgattaatac
    15721 catgacacgg gccgtgaaac cgcgcaggtt tgcaatcagg cccatcatct acgccagcgt
    15781 tcttagcgct ggcgtattgt tgtgcgcctt ttccgcccac gcggatgagc gtgaccaact
    15841 caaatctatt caggccgata tcgccacaaa agagcgcgcg gtacgccaaa agcaacaaca
    15901 acgcgcaagc ctgctcgcac aattgaaaaa gcaggaagaa gcgatctctg aagccacccg
    15961 taagctgcgc gaaacgcaaa acacgctcaa tcaactcaat aaacagattg atgagatgaa
    16021 cgcgtcgatt gccaaactgg agcagcaaaa agccgcccag gagcgcagcc tcgccgcaca
    16081 actggatgcc gcattccgtc agggcgagca taccggtatt cagctaattc tcagcggtga
    16141 agaaagccag cgtggacagc gtttacaggc ttatttcggc tatctcaacc aggcgcgaca
    16201 agaaaccatt gcccagttga agcaaacgcg tgaagaagtc gccatgcagc gtgctgaact
    16261 ggaagagaaa caaagcgagc aacaaacgct gttatatgaa cagcgcgccc aacaggcgaa
    16321 actgactcag gctctgaacg agcgtaaaaa gacgctggcg gggctggaat cttccatcca
    16381 gcaaggtcag cagcagctaa gtgaactgcg tgccaacgaa tcccgtctgc gtaacagtat
    16441 tgcccgtgcg gaagccgcgg cgaaagcgcg tgctgaacga gaagcgcgtg aggctcaggc
    16501 agttcgcgac aggcagaaag aagcgacgcg caaaggcacc acctacaaac cgaccgaaag
    16561 cgaaaaatcg ctgatgtccc gaactggtgg cctgggtgcg ccgcgtggtc aggcattctg
    16621 gccagttcgt ggccctacgc tgcatcgcta tggcgagcag ctacagggtg aattacgctg
    16681 gaaaggaatg gttatcggtg cctctgaagg tactgaagtt aaagcaattg ccgacggtcg
    16741 ggtgattctg gctgactggc tgcaaggcta cggtctggtg gtggtggttg agcacggtaa
    16801 aggcgatatg agtctttacg gctataatca aagcgcgctg gtgagcgttg gttcgcaggt
    16861 tcgtgcgggc cagccaattg cactggtggg cagcagtggc ggtcagggca gaccttcact
    16921 ctatttcgaa attcgccgcc agggtcaggc cgtcaatcca cagccgtggt tgggaagata
    16981 agttttgttt ccatttcgtc gtaacgttct tgcatttgcc gctctgttgg cgctctcctc
    17041 ccctgtaatt gctggcaaac ttgccatcgt cattgatgat tttgggtatc gcccgaacaa
    17101 cgaaaaccag gtgctggcga tgccttccgc tatctccgtc gctgtattac ccgattcacc
    17161 gcacgccaga gagatggcga ccaaagcgca taacagcggt cacgaagtgt tgattcatct
    17221 cccgatggca ccgttgagta aacagccgct ggagaaaaat acgctgcgcc cggagatgag
    17281 cagcgacgaa attgagcgca ttattcgtag tgcggtcaat aacgtgccct atgccgtggg
    17341 gatcaataac cacatgggca gcaagatgac ctctaacctg tttggtatgc agaaagtgat
    17401 gcaggcgctg gagcgttata atctttactt cctcgacagc gtaaccattg gtaataccca
    17461 ggcgatgcgc gccgcgcaag gcactggcgt gaaggtgatc aaacggaagg tgttcctcga
    17521 cgattcacag aacgaagcgg acatccgtgt gcaattcaat cgcgcaattg acctagcgcg
    17581 tcgcaacgga tcaaccattg ccattgggca tcctcaccct tcaacggtac gcgtgttgca
    17641 acagatggtt tataacctgc cgccagacat tacgctggtg aaagccagca gcttgctgaa
    17701 tgaaccgcag gttgatacgt ctacaccgcc gaaaaacact gtgcccgaca caccgcgtaa
    17761 tccgttccgt ggcgtgaagc tgtgcaaacc gaagaaaccg atagaacctg tttatgctaa
    17821 tcgcttcttt gaagtattaa gcgaaagcat cagccagagc acgctgatcg tttacttcca
    17881 gcatcagtgg caaggctggg gcaaacagcc tgaagcggcg aagcttaacg ctagcgtaaa
    17941 ttaaaggcgc tacgcgtaat gcgatggtgc gaccgcgttt tgtcgcgcca ttgccataag
    18001 cggaaagact acagtaacgc ctgataaccc acctttacgc tgcgcacatt ggtaatcagg
    18061 cgcttataca ta
//
LOCUS       DAZPJR010000075        17959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPJR010000075 DAZPJR010000000
VERSION     DAZPJR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.076"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(229..1203)
                     /gene="cysB"
                     /locus_tag="ACHVB7_002401"
     CDS             complement(229..1203)
                     /gene="cysB"
                     /locus_tag="ACHVB7_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR1914369.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1413..4010)
                     /gene="topA"
                     /locus_tag="ACHVB7_002402"
     CDS             complement(1413..4010)
                     /gene="topA"
                     /locus_tag="ACHVB7_002402"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR1914370.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4390..4641
                     /locus_tag="ACHVB7_002403"
     CDS             4390..4641
                     /locus_tag="ACHVB7_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR1914371.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="ACHVB7_002404"
     CDS             complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="ACHVB7_002404"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR1914372.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5946..6704
                     /locus_tag="ACHVB7_002405"
     CDS             5946..6704
                     /locus_tag="ACHVB7_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR1914373.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6701..7291
                     /gene="cobO"
                     /locus_tag="ACHVB7_002406"
     CDS             6701..7291
                     /gene="cobO"
                     /locus_tag="ACHVB7_002406"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR1914374.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="ACHVB7_002407"
     CDS             complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="ACHVB7_002407"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR1914375.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8416..9530)
                     /locus_tag="ACHVB7_002408"
                     /pseudo
     CDS             complement(8416..9530)
                     /locus_tag="ACHVB7_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9558..10178)
                     /locus_tag="ACHVB7_002409"
     CDS             complement(9558..10178)
                     /locus_tag="ACHVB7_002409"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR1914376.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10175..11056)
                     /gene="rnm"
                     /locus_tag="ACHVB7_002410"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(10175..11056)
                     /gene="rnm"
                     /locus_tag="ACHVB7_002410"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR1914377.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11194..11238
                     /gene="trpL"
                     /locus_tag="ACHVB7_002411"
     CDS             11194..11238
                     /gene="trpL"
                     /locus_tag="ACHVB7_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR1914378.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11215..11308
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11330..12892
                     /gene="trpE"
                     /locus_tag="ACHVB7_002412"
     CDS             11330..12892
                     /gene="trpE"
                     /locus_tag="ACHVB7_002412"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR1914379.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12892..14487
                     /gene="trpD"
                     /locus_tag="ACHVB7_002413"
     CDS             12892..14487
                     /gene="trpD"
                     /locus_tag="ACHVB7_002413"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR1914380.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14488..15849
                     /gene="trpCF"
                     /locus_tag="ACHVB7_002414"
     CDS             14488..15849
                     /gene="trpCF"
                     /locus_tag="ACHVB7_002414"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR1914381.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15861..17054
                     /gene="trpB"
                     /locus_tag="ACHVB7_002415"
     CDS             15861..17054
                     /gene="trpB"
                     /locus_tag="ACHVB7_002415"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR1914382.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17054..17860
                     /gene="trpA"
                     /locus_tag="ACHVB7_002416"
     CDS             17054..17860
                     /gene="trpA"
                     /locus_tag="ACHVB7_002416"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR1914383.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4315 a         4750 c         4488 g         4406 t
ORIGIN      
        1 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
       61 tttgaaaact ggagtttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
      121 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
      181 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
      241 cagttttata tctttaaaca tgacctcaat ttcttcatta gagcgcaatg cgacagccgc
      301 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
      361 gcgcaagaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
      421 agcatcaaca cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
      481 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
      541 gacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
      601 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
      661 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
      721 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagtga aatcagcatt
      781 gcctttagag acggcatcag caatttgtgt cggcgagccc tggtgcatat gcaaagaaac
      841 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
      901 atgcgtggtg gcgatataca gtgagccttt atccggccag gtgtgctctc cggcaaccga
      961 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgccctgc
     1021 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
     1081 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
     1141 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
     1201 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
     1261 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
     1321 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
     1381 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
     1441 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
     1501 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
     1561 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
     1621 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaaaacaccc
     1681 gcagcgccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
     1741 ggcaccggat cttctttcgg tggtgccact tcgccgttac gtaaaatctt acgtgtgttt
     1801 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
     1861 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
     1921 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
     1981 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
     2041 cgcagcgcgt tggtttcagc gtcttcgcct tccagtacgt tcagcacttc gttttccggc
     2101 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcataacc agaacagcca
     2161 aggaataccc cggtgctcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
     2221 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
     2281 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
     2341 tcgtgatttg ccaccaggtc gaggctgttt tccatctgtg cggtgaagtc gtagttcatt
     2401 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgccaatttt ttccgcatag
     2461 aaacgacgat tttctactcg cacgtagcca cgatcctgaa tggtcgaaat gatcgacgca
     2521 taggtagacg gacgaccgat accgcgtttt tccagctctt taaccagcga tgcttcactg
     2581 aaacgggctg gcggcttggt aaagtgctgg gctggtgtaa gttcaacgag cgtcagagca
     2641 tcgcctttat taactgctgg taagatgcga tcttcatcgc ctttacgcaa cgcaggcatc
     2701 acttttgtcc agccatcaaa acgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
     2761 gcaccaatgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggctacgaac
     2821 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
     2881 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
     2941 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
     3001 ccacgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
     3061 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaatcca
     3121 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
     3181 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
     3241 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg atgagtcacc
     3301 tgtaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc ccagaactct
     3361 tccggcacga acgctttaat ttcacgctca cgctcgacca ccaggcgaac cgccaccgac
     3421 tgcacacgac cggcagacag accacgagcg atctttttcc atagcagcgg cgaaaccata
     3481 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
     3541 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
     3601 actcggctat agcgcgcatc atcaccccca atcacttccc gcaggtgcca tgcaatggct
     3661 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
     3721 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
     3781 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
     3841 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
     3901 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag atacttgttg
     3961 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
     4021 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
     4081 taccgatatg gactcgttct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
     4141 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
     4201 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
     4261 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
     4321 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
     4381 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
     4441 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
     4501 cagcgtaatg gcgtgctggt atttatcggc gactactttt tagatgaaca aggattgcca
     4561 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
     4621 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
     4681 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
     4741 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
     4801 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
     4861 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
     4921 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
     4981 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
     5041 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
     5101 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
     5161 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
     5221 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
     5281 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
     5341 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
     5401 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
     5461 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
     5521 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
     5581 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
     5641 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt cgccaaaaac
     5701 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
     5761 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
     5821 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
     5881 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
     5941 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
     6001 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
     6061 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
     6121 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
     6181 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
     6241 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
     6301 acgtcatgca ggtcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
     6361 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttggg cgtcagggac
     6421 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
     6481 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
     6541 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
     6601 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
     6661 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
     6721 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
     6781 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
     6841 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
     6901 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
     6961 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
     7021 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
     7081 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
     7141 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
     7201 tcttgaactg gcggatacgg taagtgagct gcgccccatc aaacatgcgt ttgatgccgg
     7261 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
     7321 catactttga ttaaccgtta ttacgcccgc cagaacgacg accgccgctc acctgactgt
     7381 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
     7441 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
     7501 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
     7561 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
     7621 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
     7681 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
     7741 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
     7801 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
     7861 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
     7921 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
     7981 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
     8041 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
     8101 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
     8161 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
     8221 tcgccttccc aggcgtctga tatgcttttg tgcaacagtg agtcagaact tacatctgac
     8281 tcactgatag taaagtctgt ttgaactctt caggccttaa gtaaacgact cgacgtttta
     8341 actggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     8401 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc caccagcacc
     8461 accaccggaa taacttcgcc ccaacaaact ggttttggcc cccagactca ggtgcatctg
     8521 taatgaaaac aaaacgccac tacgggcaat ttccggggcg ctaacagccg ctgtgagtga
     8581 tggggtgata cgtgctaccc attctcttcc aaggtttgct gagactgccc aacctagcaa
     8641 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgc ccactgtgtg ttgctgcctc
     8701 tttacgcgca agatagttaa cgatagcttc accgtgggca taacgttgct gcccagtttg
     8761 tgtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggaaaacagc
     8821 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatgtagag
     8881 agaatagaag gccacgcctg aacaaatcag tggtaaacaa ttcttccgtc ctcaaggatg
     8941 cggccatttc gatatgtgtc gccctcttct ccagtaaaga gatcgatttc gctaaatgga
     9001 ttacccgcga tatcagggaa cttgagtgaa aatcgggtct tatagatttc aaacggccag
     9061 tggttaccag tcacgttcca gattaacaga tctgaatctc cctcacgcag gaaagcattt
     9121 ttaacctgat aatgaatttg ataatgatag tcgccttgag gcaagaaacg ttgtttatcg
     9181 cccacgacaa tactcattaa cccgatatgc cagtcatgat gccagggttc aggaatacca
     9241 tcacgggtga cgccaacaat atgatagttc agcagtgcag cgtccccacc aggctgcatc
     9301 cagagtcgtg gtaaatcacg ataaaatccg tgtttaatct gctgatttcg cgcctgaaaa
     9361 tgaatatttt ctttgatgtc gagcacccca tccggttgca gagtggcttc aacatcaaag
     9421 agcgaaatcc cttcttcata agaagggatc tccgtgtttt ctgcataact tagagatgat
     9481 acaaatagcc ctgctatcaa cagcaaaatg cagcgaaact tccctgccat agtcttccat
     9541 ccgtggaacg caatttgtta taagaaaggc ttcacatcac ctacgccttc acgcaccacg
     9601 accggcgtat catcggtaag atcaataacc gttgtcggtt tctgcccgag atagccgccg
     9661 tgaataatca aatctacctg tttttccaga cggtctttaa tttcttcggg atcggattcg
     9721 gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc cagtgcttca
     9781 agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt gcgtttttcc
     9841 tgcaacagac ggcgtggcac ttccttcgtc cccttcagga tgaaggtata gttgcccggc
     9901 gtgttgtttt tcattaaacg aaacgcaacg ttatcaacaa atgaataggt cgagagttca
     9961 gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg aatacgacaa
    10021 atacgctcca tcgcgttttt atcttcaatt ttacagccga gcgcatagcc ggaatcagtt
    10081 ggataaacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat cagacgttgc
    10141 tgtgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct gtggtgttct
    10201 gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag agtttacgac
    10261 ctagttcaat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc cataaatgat
    10321 gctgacgcgc aagggccgcc agctgggtac gttcattggg cgattgctgg cactgcgcga
    10381 cttccatcgc gtcaccgtgg tgttcggcaa aatgcgcaac caatcttttc agccatttag
    10441 cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa tgatgaatga
    10501 catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt ttcccgcgcg
    10561 ccagatattt tttaaagaca tccgccattg aactggcttt gccgcacccc acaaggaaac
    10621 gcgcaaaatg accgcgcgtc accgcaccgc cctgcgccag tcgtcgcgcg ccttccagtg
    10681 cccctggtat ttgcgctttt tcaagtcttt cggcaatcag ttgcgcccgc tgattgcgcc
    10741 gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca atattcagcc
    10801 cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg ggaataagat
    10861 tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cacgattgca gctgtgttgt
    10921 catggtcggt gatcgccagg gtaccgacgc gcatctcgac tgcacggtgc accaatactt
    10981 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcgt
    11041 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
    11101 acagttctgg caaatattct gaaatcagct gttgacaatt aatcatcgaa ctagttaact
    11161 agtgcgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt actgaaagct
    11221 tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa tcagataccc
    11281 agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa tgcaaacaca
    11341 aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaacc cgactgcgct
    11401 ttttcaccag ttgtgtggga atcgtccggc aacgctgctg ctggaatccg cagatatcga
    11461 cagcaaagat gatttaaaaa gcctgctact ggtagacagt gcgctgcgca ttacagcatt
    11521 aggtgacact gtcacaattc aggcactttc cggcaacggc gaagccctgc tggcactact
    11581 ggataacgcc ctgcctgcgg gtgtggaaaa tgaacaatca ccaaactgcc gtgtgctgcg
    11641 cttcccccct gtcagtccac tgctggatga agacgcccgc ttatgctccc tttcggtttt
    11701 tgacgctttc cgtttattgc agaatctgtt gaatgtaccg aaggaagaac gagaagcaat
    11761 gttcttcggc ggcctgttct cttatgacct tgtggcggga tttgaagatt taccgcaact
    11821 gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaacgc tgatggtgat
    11881 tgaccatcag aaaaaaaaca cccgcattca ggccagcctg tttgctccga atgaagaaga
    11941 aaaacaacgt ctcactgctc gcctgaacga tcttcgccag cagctgaccg aagccgcgcc
    12001 gccgctgccg gtggtttccg tgccgcatat gcgttgtgaa tgtaatcaga gcgatgaaga
    12061 gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gctggagaaa ttttccaggt
    12121 ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct attacgtgct
    12181 gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt tcaccctgtt
    12241 tggcgcgtcg ccggaaagtt cgctcaagta tgacgccacc agccgccaga ttgagatcta
    12301 cccgattgcc ggaacacgtc cacgcggtcg tcgtgccgat ggctcgctgg acagagacct
    12361 cgacagccgc atcgaactgg aaatgcgtac cgatcataaa gagctttctg aacatctgat
    12421 gctggtggat ctcgcccgta atgatctggc acgcatttgc acccccggca gcagctacgt
    12481 cgccgatctt accaaagttg accgttactc ttacgtgatg cacctggtct cccgcgtggt
    12541 cggtgagctg cgccacgatc tcgacgccct gcacgcttac cgcgcctgta tgaatatggg
    12601 gacgttaagc ggtgcgccga aagtacgcgc catgcagtta attgccgagg cggaaggtcg
    12661 tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcatggcg atctcgacac
    12721 ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gccaccgtgc aagccggtgc
    12781 tggcgtagtc cttgattcta ttccgcagtc ggaagccgac gaaacccgta ataaagcccg
    12841 cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagactttct gatggctgac
    12901 attctgctgc tcgataatat cgactctttt acgtacaacc tggcagatca gttgcgcagc
    12961 aatgggcata atgtggtgat ttaccgcaac catattccgg cgcagacctt aattgaacgc
    13021 ctggcgacga tgagcaatcc gatactgatg ctttctcctg gccccggtgt gccgagcgaa
    13081 gccggttgta tgccggaact cctcacccgc ttgcgtggca agctgcccat tattggcatt
    13141 tgcctcggac atcaggcaat tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa
    13201 attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt tgccggatta
    13261 acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaacat tccggccggt
    13321 ttaaccatca acgcccattt taatggcatg gtgatggcag tacgtcacga tgcggatcgc
    13381 gtttgtggat tccagttcca tccggaatcc attctcacca cccagggcgc tcgcctgctg
    13441 gaacaaacgc tggcctgggc gcagcagaaa ctagagccag ccaacacgct gcaaccgatt
    13501 ctggaaaaac tgtatcaggc gcagacgctt agccaacaag aaagccacca gctgttttca
    13561 gcggtggtgc gtggcgagct gaagccggaa caactggcgg cggcgctggt gagcatgaaa
    13621 attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa ccgcgctact ggaaaacgca
    13681 gcgccgttcc cgcgcccaga ttatctgttt gctgatatcg tcggtactgg cggtgacggc
    13741 agcaacagta tcaatatttc taccgccagt gcgtttgtcg ccgctgcctg tgggctgaaa
    13801 gtggcgaaac acggcaaccg tagcgtctcc agtaaatctg gttcgtccga tctgctggcg
    13861 gcgttcggta ttaatcttga tatgaacgcc gataaatcgc gccaggcgct ggatgagtta
    13921 ggtgtttgtt tcctctttgc gccgaagtat cacaccggat tccgccacgc gatgccggtt
    13981 cgccagcaac tgaaaacccg caccctgttc aatgtgctgg ggccattgat taacccggcg
    14041 catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc gattgccgaa
    14101 accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg gatggatgaa
    14161 gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcgaaat taagagctat
    14221 caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact ggcaggcgga
    14281 acaccggaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg cgacgccgcc
    14341 catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg ccatgaagat
    14401 ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc ttacgacaga
    14461 gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca
    14521 gacaaggcga tttgggtaga aacccgcaaa cagcagcaac cgctggccag ttttcagaat
    14581 gaggttcagc cgagcacgcg acatttttat gatgcgctac agggtgcacg cacggcgttt
    14641 attctggagt gcaagaaagc gtcgccgtca aaaggcgtga tccgtgatga tttcgatccg
    14701 gcacgcattg cctccattta taaacattac gcttcggcaa tttcggtgct gactgatgag
    14761 aaatattttc aggggagctt tgatttcctc cccatcgtca gccaaatcgc cccgcagccg
    14821 attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg ctattaccag
    14881 gccgatgcct gcttattaat gctttcagta ctggatgacg accaatatcg ccagcttgca
    14941 gccgtcgctc acagtctgga gatgggggtg ctgaccgaag tcagtaatga agaggaactg
    15001 gagcgcgcca ttgcattggg ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat
    15061 ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca caacgtgacg
    15121 gtaatcagcg aatccggcat caatacttac gctcaggtgc gcgagttaag ccacttcgct
    15181 aacggttttc tgattggttc ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc
    15241 cgggtgttgc tgggtgagaa taaagtatgt ggtctgacgc gtgggcaaga tgctaaagca
    15301 gcttatgacg cgggcgcgat ttacggtggg ttgattttgg ttgcgacatc accgcgttgc
    15361 gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg
    15421 ttccgcaatc acgatattgc cgatgtggtg gacaaagcta aggtgttatc gctggcggca
    15481 gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga agctctgcca
    15541 gcacacgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc ccgcgagctt
    15601 cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca acgtttcgac
    15661 tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg cttaggcgca
    15721 gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa ttctgctgta
    15781 gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc
    15841 gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt gagtttggcg
    15901 gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca
    15961 gcgcgcaaaa agatcctgaa tttcaggccc agttcaacga cctactgaaa aactatgccg
    16021 ggcgtccaac cgcactgacc aaatgccaga acattacggc tggcacgaac accacgctgt
    16081 atctgaagcg cgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctcggtc
    16141 aggctttact ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc
    16201 agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata
    16261 tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg
    16321 cggaagtgat cccggtgcat agcggttccg cgacgctgaa agatgcctgt aacgaggcgc
    16381 tgcgcgactg gtccggtagt tacgaaaccg cgcactatat gctgggcacc gcagctggcc
    16441 cgcatcctta tccgaccatt gtgcgtgagt ttcagcggat gattggcgaa gaaaccaaag
    16501 cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg
    16561 gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc ggcctgattg
    16621 gtgtggagcc aggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg
    16681 gtcgcgtggg tatctatttc ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga
    16741 ttgaagaatc ttactccatc tccgccggat tggatttccc gtctgtcggc ccacaacacg
    16801 cgtatcttaa cagcactgga cgcgctgatt acgtgtctat taccgatgat gaagcccttg
    16861 aagccttcaa gacgctgtgc ctacacgaag ggatcatccc ggcgctggaa tcctcccacg
    16921 ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga aaaagagcag ctactggtgg
    16981 ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac
    17041 gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga
    17101 aggcgcattc gttcctttcg tcacgctcgg ggatccgggc attgagcagt cattgaaaat
    17161 tatcgatacg ctaattgaag ccggtgctga cgcgctggag ttaggcatcc ccttctccga
    17221 cccactggca gatggcccga cgattcaaaa cgccactctg cgcgcctttg cggcaggtgt
    17281 gactccggca caatgttttg aaatgctggc actgattcgc cagaaacacc cgaccattcc
    17341 cattggcctg ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg agttttatgc
    17401 ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccaa ttgaagagtc
    17461 cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca cctatcttca tctgcccgcc
    17521 aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt acacctattt
    17581 gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc tcaatcatct
    17641 ggttgcgaag ctgaaagagt acaacgctgc acctccattg caggggtttg gtatttccac
    17701 cccggatcag gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt ctggttcagc
    17761 cattgttaaa atcatcgagc aacatattaa tgagccagag aaaatgctgg cggcactgaa
    17821 agtttttgta caaccgatga aagtggcgac gcgcagttaa tccccctgcc gccagttccg
    17881 ctggcggcat tttaactttc tttaacgaag ccggaaaaat cctaaattca tttaatattt
    17941 atctttttac cgtttcgct
//
LOCUS       DAZPJR010000076        17852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPJR010000076 DAZPJR010000000
VERSION     DAZPJR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.077"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHVB7_002417"
     CDS             complement(<1..304)
                     /locus_tag="ACHVB7_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR1914384.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(514..1032)
                     /locus_tag="ACHVB7_002418"
     CDS             complement(514..1032)
                     /locus_tag="ACHVB7_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR1914385.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1729..2229
                     /gene="tssB"
                     /locus_tag="ACHVB7_002419"
     CDS             1729..2229
                     /gene="tssB"
                     /locus_tag="ACHVB7_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR1914386.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2264..2488
                     /locus_tag="ACHVB7_002420"
     CDS             2264..2488
                     /locus_tag="ACHVB7_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914387.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2539..4014
                     /gene="tssC"
                     /locus_tag="ACHVB7_002421"
                     /pseudo
     CDS             2539..4014
                     /gene="tssC"
                     /locus_tag="ACHVB7_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            4021..4434
                     /gene="tssE"
                     /locus_tag="ACHVB7_002422"
     CDS             4021..4434
                     /gene="tssE"
                     /locus_tag="ACHVB7_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR1914388.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4438..6288
                     /gene="tssF"
                     /locus_tag="ACHVB7_002423"
     CDS             4438..6288
                     /gene="tssF"
                     /locus_tag="ACHVB7_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR1914389.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6252..7334
                     /gene="tssG"
                     /locus_tag="ACHVB7_002424"
     CDS             6252..7334
                     /gene="tssG"
                     /locus_tag="ACHVB7_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR1914390.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7359..8639
                     /gene="tagH"
                     /locus_tag="ACHVB7_002425"
     CDS             7359..8639
                     /gene="tagH"
                     /locus_tag="ACHVB7_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR1914391.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8636..9160
                     /gene="tssJ"
                     /locus_tag="ACHVB7_002426"
     CDS             8636..9160
                     /gene="tssJ"
                     /locus_tag="ACHVB7_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR1914392.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9163..10492
                     /gene="tssK"
                     /locus_tag="ACHVB7_002427"
                     /pseudo
     CDS             9163..10492
                     /gene="tssK"
                     /locus_tag="ACHVB7_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10497..11258
                     /gene="icmH"
                     /locus_tag="ACHVB7_002428"
     CDS             10497..11258
                     /gene="icmH"
                     /locus_tag="ACHVB7_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR1914393.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11267..14050
                     /gene="tssH"
                     /locus_tag="ACHVB7_002429"
                     /gene_synonym="clpV"
                     /pseudo
     CDS             11267..14050
                     /gene="tssH"
                     /locus_tag="ACHVB7_002429"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            14047..14790
                     /gene="vasI"
                     /locus_tag="ACHVB7_002430"
     CDS             14047..14790
                     /gene="vasI"
                     /locus_tag="ACHVB7_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR1914394.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14795..16207
                     /gene="tssA"
                     /locus_tag="ACHVB7_002431"
     CDS             14795..16207
                     /gene="tssA"
                     /locus_tag="ACHVB7_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR1914395.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16226..>17852
                     /gene="tssM"
                     /locus_tag="ACHVB7_002432"
     CDS             16226..>17852
                     /gene="tssM"
                     /locus_tag="ACHVB7_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFR1914396.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPY"
BASE COUNT         4155 a         4894 c         4877 g         3926 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 tcgtcaggta ggccattttg tccagtacct gcggaaattc aagggagaga aactgctggc
      181 tgaccagaga gagctcgagg gaaaagagcg aagagagtga ctggttcaga tgaaaggaga
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
      301 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
      361 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca tccgccatct
      421 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
      481 tccggcctga tagccggatg ctgttggctt aacttatgct tccagtggtg cgcgccagtc
      541 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
      601 aacctcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
      661 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
      721 ctccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
      781 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
      841 agcacgctga cccgcaggct gaccggactg aggagtggtc gggacagtaa cgttatgcag
      901 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
      961 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     1021 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     1081 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     1141 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     1201 aaatctctta ttcagcaata acttaataat atatatctgg ataaccatac ccaactgttc
     1261 aaagtccgta ttgcctccat aaaatggcca aagctgaatc atgaataaat attgtattta
     1321 tcctttctga attatcattt tgataatttt tgagatattc cttaaaaata cccacttaat
     1381 caatcacaaa acaaatatat aaacaagcaa tcattagatt agcttatttt taatgaaatt
     1441 attatatact tcaataagtt acaaaaaaac tctatattta aattattgcc ataatacacc
     1501 tgaagcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
     1561 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
     1621 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
     1681 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
     1741 tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat cgtatgtccc aaaaacagat
     1801 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
     1861 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
     1921 ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc cacgctgaag
     1981 ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact ggatgatttt
     2041 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
     2101 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
     2161 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg tctggtgagc
     2221 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
     2281 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
     2341 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
     2401 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     2461 gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc ggtttcagaa acacagctaa
     2521 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     2581 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     2641 ggttcggaag gttatgacgt tgccctccag ggtgtgacag cctttattgc cagcatcctg
     2701 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     2761 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     2821 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     2881 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     2941 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     3001 ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     3061 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     3121 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     3181 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     3241 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     3301 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     3361 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     3421 taccgctgat gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     3481 atgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     3541 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     3601 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     3661 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     3721 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     3781 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     3841 aacccgcctg ctgacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     3901 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     3961 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     4021 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     4081 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     4141 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     4201 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     4261 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     4321 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     4381 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     4441 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     4501 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc agatccggat
     4561 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     4621 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     4681 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     4741 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     4801 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     4861 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     4921 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     4981 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     5041 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     5101 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgct gccctggccg
     5161 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     5221 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     5281 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     5341 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     5401 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     5461 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     5521 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     5581 catcagatag aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     5641 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     5701 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     5761 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg aaaaaatgcg
     5821 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     5881 ggcagcctgc actggcccct actctccgcc atgaacctga attatctgtc attactggat
     5941 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     6001 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     6061 gatcgcctgt ttacgggtat tcccgtccgg gggctggctt ccacgctgta tctgcacccg
     6121 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     6181 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     6241 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     6301 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     6361 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     6421 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     6481 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     6541 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     6601 tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     6661 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     6721 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     6781 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     6841 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     6901 gctttgattt gtcagaagtg acactgcaaa actggcaacg tcgcaaggtg gatattgaac
     6961 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     7021 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     7081 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     7141 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     7201 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     7261 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     7321 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     7381 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     7441 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     7501 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     7561 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     7621 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     7681 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     7741 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     7801 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     7861 tccaggctct tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     7921 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     7981 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtttctgcgg
     8041 cagaaatggc acaacgccac gttgcggtca cccccttact gcgcggtctt ggcggctcgc
     8101 ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     8161 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     8221 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     8281 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     8341 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     8401 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     8461 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     8521 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     8581 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     8641 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     8701 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     8761 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     8821 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     8881 agccgactat gaccagatag ccagcacgcc gctaccggat gtgctgggta aaaactatat
     8941 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     9001 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     9061 atggaaacaa atcgagacgg tggaaggaat cggccaccat taccgcctgc tggtgcatgt
     9121 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     9181 aaggtgatgt ggcaggaagg gatgctgatg cgtccacacc atttccagca gcagcagcgt
     9241 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     9301 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     9361 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     9421 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     9481 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     9541 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgctg
     9601 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     9661 ccgctttgcc gtatcagcga tcgccgccga tggctccctg acgttggatg acgattttat
     9721 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     9781 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     9841 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     9901 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     9961 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
    10021 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
    10081 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
    10141 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
    10201 cgtggcggca aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
    10261 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atccccggta ttccattgcg
    10321 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
    10381 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
    10441 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
    10501 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
    10561 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
    10621 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
    10681 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
    10741 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
    10801 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
    10861 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
    10921 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
    10981 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
    11041 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
    11101 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
    11161 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
    11221 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
    11281 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
    11341 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
    11401 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    11461 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    11521 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctagctgc tggcgtcggc
    11581 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    11641 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    11701 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    11761 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    11821 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    11881 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccag tggtggtggg
    11941 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    12001 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    12061 ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    12121 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    12181 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    12241 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    12301 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    12361 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    12421 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    12481 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    12541 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    12601 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    12661 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    12721 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    12781 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    12841 agagtcagag tcagagtcag agtcagagca ggataatacc ggtgccgaac cggctgatga
    12901 ggccgacaga gaacaaccgg aagagaccgc tgaaacagtt tccccggtac agcggctggc
    12961 ccagctcact gccgaactgg acgccctgca taacgaccgg ttgctggtct ccccgcacgt
    13021 cgataaaaaa cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc tcaaccgcct
    13081 gtcgcagaat gaaatgtcgg tcatcaccga cctgacgaaa tggctgggtg acaccatcaa
    13141 aggccaggac ctggcgattg ccagcctgca taagcatcta ctgaccgcac gcgccgacct
    13201 gcgtcgtccg ggacgcccgc tcggtgcgtt cctgctggct ggccccagcg gtgtgggtaa
    13261 aaccgaaacc gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac
    13321 catcaatatg tccgaattcc aggagaaaca caccgtctcg cggctgattg gttcgcctcc
    13381 gggctacgtt ggctacggtg aaggcggcgt actgaccgaa gcgattcgcc agaaacccta
    13441 ctcggtagta ctgctcgatg aagtggaaaa agcgcacccg gatgtgctca acctgttcta
    13501 ccaggcgttc gataagggcg aaatggcaga cggtgaaggc cgcctgattg actgcaaaaa
    13561 tatcgtcttc ttcctgacgt ccaacctcgg ctaccaggta atagtcgagc atgccgatga
    13621 cccggaaacc atgcaggaag tacagtatcc ggtgctggcc gacttcttca aacctgccct
    13681 gctggcgcgt atggaagtgg tgccgtatct gccgctgtcg aaagagacgc tcgccaccat
    13741 tatcgccgga aaactggccc gtctggataa cgtgctgcgc agtcgctttg gtgtagaagt
    13801 ggtcattgaa ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg
    13861 cgcgaggatg ctggaatcgg tcatcgatgg cgacatgcta ccgccgctct cgctgctgct
    13921 gttgcagaaa atggcggcta acacggcgat tgcccggatt cggttgtcgg cagtggacgg
    13981 cgcatttacg gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac
    14041 ggttttatga gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg
    14101 acgggcttac ccgccactgc cgcgcccgca gaagcatcgt caatggccgg tgtggcggtc
    14161 gccgtcgcca ccacgacccc gccggatgcg acagccacgt tacaggccat gcagtcctgt
    14221 cggcgggagt ccgcggcgct ggagcggctt gactgctatg accgcctgct ggcaccgctg
    14281 tccccgtcag ggtttgacgg ggcgctggtc aaagccggtt ttgtgggcga ggcctggacc
    14341 cgcgccacag agcaggaaaa acgccgcgag ggcaacacca cagaactgct ggtgacgcag
    14401 gtgccggggg agcgcccgac ggtggtgata accaccccgg ctatcggtca tgttccgcca
    14461 cgcccggtgc tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg
    14521 caccctctgg atgtccatga tatcgccgtc actctgaatg ccgacaaccg ggcattgcgc
    14581 agccactggt ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gttatccggg
    14641 attgatgaaa ttaagcaact gttcggcgca aaaacgctga cggtggatac cggcgcagac
    14701 aatgccgccg gaaagctgac ctttaacatt gacgggctgg caagggcgat tgctcccctg
    14761 cgcgatgcct gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa
    14821 tgtctggata tcgcacctgc tggaaaacct gccggaagag aaactggcat cggcgctgaa
    14881 agatgacaac ccgaactggg agtatatcga cggcgaaatc gtcaagctgg gatcgctggc
    14941 ccacagtcaa ctggatatcc cggaactcca gcgccggggg ctggtcattc tggcttcgga
    15001 gagtaaggat tttcgtctgc tggcgcatct gctaagaacc ctgcaacacg ccggtgatcc
    15061 tcatctcgca ttgcgcctgc tggcgctgta tgtggagcat tactgggcgg tggctgcgcc
    15121 gcagaatatg gcgcataaaa aacgctttgc cagccaggtc attaaacgct ttgaaacagg
    15181 tattgaagtt ttttcacaga acgccgccac agcgcagcgc gatgcgctgt caggggaact
    15241 ggcaaaactg gcgcaatgct ggcagtcaca taacgccccg gaactggcac aggctactga
    15301 tgatcttttt gccctgtacc agcgtgcgtt taatcgtgcg gcaccggctc ctgtccccac
    15361 tccggcggcc tccggtagtt caccacagac caccgccacg tctgaaagcg gcgtgacgca
    15421 acccagtgct ccggctcccc aaatcgtcat cgacagtcac gacgacaaag cctggcgcga
    15481 cacgctgtta aaagtggcgg ccattttatg tgaacgccag ccggattcac cgcagggcta
    15541 ccgtctgcgc cgccatgcgc tttggcagaa catcaccagt acgccccagg cggaaagcga
    15601 tagccgtact ccactggctg cggtctccgc cgatatggtg gcagattacc atgctcagct
    15661 cggtagtgcc gatatggcgc tgtggcagca ggtggagaaa agcgtactgc tggctcctta
    15721 ctggctggat ggtcactgcc tttctgcaca gacggcgctg cgtctgggtt acaaacaggt
    15781 ggcagacgcc atccacgatg aggtcatccg cttccttgag cgtctgcccc agcttaccgg
    15841 gctgctgttt aatgaccaca caccgtttat cagtgagcag acgaaacaat ggctggcagc
    15901 ttcgcccgac gccaaagttg caccggttgc gcaaatcggt gaggaatcga aggcagccag
    15961 agcctgtttt gctgagcagg gtctggaggc ggcgctgcga tatctggaca tgctacccga
    16021 aggcgatccc cgcgatcagt ttcaccgcca gtaccttgcc gcacagttga cggaagaggc
    16081 ggggctggta cagcttgcgc agcagcagta ccggatgttg ttcaggatgg gattgcagat
    16141 gatggtggca gactgggagc cgtccctgct tgagcagcta gaacaaaaat tcacggcaga
    16201 acaataaaag agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg
    16261 actcaaatca gccctgcgac cggcgatgcc ccggtttaaa gtctctgctt tctggctgct
    16321 gatactggcg tggatttttc tgctggtgtg gatctggtgg aaaggcccga tgtggacgct
    16381 gtatgaagaa cagtggctca aaccactggc gaaccgctgg ctggcaacgg cggcgtgggg
    16441 gattattgcc ctggtgtggc ttaccgtccg ggtgatgaag cgcctgcaac agcttgaaaa
    16501 aatgcaaaag caacagcgcg aggaagccgt tgatccgctc agcgtggaac tgaacgccca
    16561 gcagcgttat cttgaccgct ggctgctgcg cctgcaacgc catctcgaca accgccgttt
    16621 cctgtggcag ttgccgtggt atatggtcat cggcccggcg ggcagtggca aaacgacact
    16681 gctgcgcgag gggtttccgt ccgacattat ttatgccccg gagggcgcac gcggcgcaga
    16741 acaacgcctg tacctcacgc cccatgtcgg taaacaggcg gtgatctttg atatcgacgg
    16801 cacactctgc gctcccgctg atgcggatat cctgcatcgc cgtctgtggg aacatgccct
    16861 cggctggctg aaagaaaagc gcgcgcgcca gccgctgaac gggattattc tgacactcga
    16921 tttacccgat ctgctgaccg cagacaaacg ccgccgcgag catctgttac agacgctgcg
    16981 cagccgctta caggatatac gccagcatct tcactgccag ttaccggttt acgtggtact
    17041 gacccggctt gatttattac agggttttgc cgccctgttc cagtcactca acagacagga
    17101 tcgcgatgcg attctgggcg tcacgttcac ccgccgtgcc catgaaaatg atgactggcg
    17161 aacagagttg aatgctttct ggcagacatg ggtggatcga atgaatctgg cgttgccgga
    17221 tctgatggtc gctcagactc acacccgcgc gtctttattc agtttttccc gccagatgca
    17281 gggaagccgt gaaccgctgg tgtcactgct tgagggtctg cttgatggcg aaaatatgaa
    17341 cgtgatgctg cgtggtgtct atctcacctc ttcgcttcag cgtggacaga tggatgatat
    17401 attcacccag tctgccgccc gccagtaccg gctgggcaat aacccactgg cgtcctggcc
    17461 cctggtggac accgcgcctt atttcacccg cagcctgttc ccgcaggcat tactcgcaga
    17521 gcctaatctg gcaacagaga gccgcgcctg gctgatacgt tcccgtcgcc gcctgacggt
    17581 tttctctgcc acaggcggcg tggcagcact gctgctcatc accggctggc atcactatta
    17641 caacggtaac tatcagtccg gcatcaccgt gcttaagcag gccaaagcct ttatggacgt
    17701 gccgcctccg cagggggaag atgactttgg caacctgcaa ctgccgctcc tgaacccggt
    17761 acgcgatgcc acactggcct atggcgactg gggcgaccgc agccgtctgg ccgatatggg
    17821 actgtaccag ggacgacgta tcgggcctta tg
//
LOCUS       DAZPJR010000077        17489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPJR010000077 DAZPJR010000000
VERSION     DAZPJR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.078"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..139
                     /locus_tag="ACHVB7_002433"
     CDS             <1..139
                     /locus_tag="ACHVB7_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFR1914397.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
     gene            97..774
                     /gene="bglJ"
                     /locus_tag="ACHVB7_002434"
     CDS             97..774
                     /gene="bglJ"
                     /locus_tag="ACHVB7_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR1914398.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(812..1600)
                     /gene="fhuF"
                     /locus_tag="ACHVB7_002435"
     CDS             complement(812..1600)
                     /gene="fhuF"
                     /locus_tag="ACHVB7_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR1914399.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            1741..1977
                     /locus_tag="ACHVB7_002436"
     CDS             1741..1977
                     /locus_tag="ACHVB7_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR1914400.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2016..2102)
                     /locus_tag="ACHVB7_002437"
     tRNA            complement(2016..2102)
                     /locus_tag="ACHVB7_002437"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2066..2068),aa:Leu,seq:cag)
     gene            complement(2370..3401)
                     /gene="rsmC"
                     /locus_tag="ACHVB7_002438"
     CDS             complement(2370..3401)
                     /gene="rsmC"
                     /locus_tag="ACHVB7_002438"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR1914401.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            3504..3917
                     /gene="holD"
                     /locus_tag="ACHVB7_002439"
     CDS             3504..3917
                     /gene="holD"
                     /locus_tag="ACHVB7_002439"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR1914402.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            3886..4332
                     /gene="rimI"
                     /locus_tag="ACHVB7_002440"
     CDS             3886..4332
                     /gene="rimI"
                     /locus_tag="ACHVB7_002440"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1914403.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4347..5024
                     /gene="yjjG"
                     /locus_tag="ACHVB7_002441"
     CDS             4347..5024
                     /gene="yjjG"
                     /locus_tag="ACHVB7_002441"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR1914404.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5115..6704
                     /gene="prfC"
                     /locus_tag="ACHVB7_002442"
     CDS             5115..6704
                     /gene="prfC"
                     /locus_tag="ACHVB7_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR1914405.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7097..7702
                     /gene="osmY"
                     /locus_tag="ACHVB7_002443"
     CDS             7097..7702
                     /gene="osmY"
                     /locus_tag="ACHVB7_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR1914406.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            7829..7990
                     /locus_tag="ACHVB7_002444"
     CDS             7829..7990
                     /locus_tag="ACHVB7_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR1914407.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8112..9185
                     /locus_tag="ACHVB7_002445"
     CDS             8112..9185
                     /locus_tag="ACHVB7_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR1914408.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9182..9964
                     /locus_tag="ACHVB7_002446"
     CDS             9182..9964
                     /locus_tag="ACHVB7_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1914409.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(10480..11343)
                     /locus_tag="ACHVB7_002447"
     CDS             complement(10480..11343)
                     /locus_tag="ACHVB7_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR1914410.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(11315..12865)
                     /locus_tag="ACHVB7_002448"
     CDS             complement(11315..12865)
                     /locus_tag="ACHVB7_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR1914411.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13123..13902
                     /gene="deoC"
                     /locus_tag="ACHVB7_002449"
     CDS             13123..13902
                     /gene="deoC"
                     /locus_tag="ACHVB7_002449"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR1914412.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14029..15351
                     /gene="deoA"
                     /locus_tag="ACHVB7_002450"
     CDS             14029..15351
                     /gene="deoA"
                     /locus_tag="ACHVB7_002450"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR1914413.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15403..16626
                     /gene="deoB"
                     /locus_tag="ACHVB7_002451"
     CDS             15403..16626
                     /gene="deoB"
                     /locus_tag="ACHVB7_002451"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1914414.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            16706..17425
                     /gene="deoD"
                     /locus_tag="ACHVB7_002452"
     CDS             16706..17425
                     /gene="deoD"
                     /locus_tag="ACHVB7_002452"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR1914415.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4165 a         4491 c         4741 g         4092 t
ORIGIN      
        1 ggcaattcac agcaacgctg aaatgtatgc atggataaat agcgcgcagg gtgcaagaga
       61 acttaacttg ccttctgttt atggagatgc cgcagaatgg aacacagccg aattaagaag
      121 agaaatgtcg cactcataga aaaatgcgtc atgagcagta tcggtattga gagtttattc
      181 agaaagtttg cgggtaaccc ttataagctc catacctata ccagtcagga gtcatttcag
      241 gatgccatgt cgcggatctc atttgcggcg gtcatttttt ctttttctgc catgagaagt
      301 gagcgcagag agggattatc ttgcctgact gaactggcga ttaagtttcc gcgtacccgg
      361 cgtttagtta ttgcggatga tgatattgaa gctcggctga ttggttcatt gtcgccatca
      421 ccgctggacg gtgtattaag taaagcgtca acgctggaga tttttcatca ggaactcttt
      481 ttgtcattaa atggtgtacg tcaggcgacc gaccgactga acaatcagtg gtacattaac
      541 caaagccgga cgctaagccc gacggagaga gaaatattgc gctttatgtc gcgtggctac
      601 tcaatgacac aaattgccga gcagcttaaa cgcaatatca aaacgatccg tgcacataaa
      661 tttaatgtga tgtcgaaact gggcgtcagt tctgatgcag ggttgttgga ggccgcagat
      721 attctgttat gtatgcggca ttgcgaagca agtaatgtgt tgcatcccta ttaatccgca
      781 tgatgccggg tttacttccc cggcagtgct ttcatttcag cgtacaatcg ccacattgct
      841 gcacatccgg taagcgataa cgctggcagc aggttcggcg caccagcagg ccatcgcgca
      901 gcacgacggt acgccagagt ggattatctt caccgttcgt gagcgttttc tcaaaaaaga
      961 gggcatggcg cagcgattca accgtcgcct cgccgagcag ttgcttcatc tcagtgagat
     1021 accagttgat caaataaccg gtattactcc agataagttt gccgttgatc tctccggtcg
     1081 cttctagtgc ctgcacaacc ggaaccagcg cctggctgat taacgtttcc attcgctgct
     1141 gcggcgaatg tggtgttgcg tttttatctt cacacacatc gacccagaaa caggcgacgc
     1201 gtccggtttc gtgaaactca gcatggaaat gttccggcga cacatctaat gccttttcct
     1261 gcgtcagtag cgccagcatt aatggtggca ccatcaggcc gatatacaat tgtgcccata
     1321 gtgagatcag cggtttgttc tcgcggatca tcgttggttg gttgcgatag atatgatcgg
     1381 aatagaccgc gagtagagaa cttagcgcat tcggtgatga ccattgcgcc agcgtcatgg
     1441 cgttaagcgg agcgggttca tccaggcgga taaactcgag caaatgttca cgatgtttgg
     1501 cgatcgtcgc acgtacggct tgtgcaagcc cagcatcctg cggctggaga tgcgttcgcc
     1561 agatgatatc ttcatagagc ggtgcggaac gataggccat aatcgggata gtaatctaaa
     1621 tgataatgat tgctaatcct agcgataggt ttacccgata gcaagggatt tctctggctt
     1681 gcaaatgata aaaattatca tatgatattg gttatcatta tcaatgaaag agatgaaacc
     1741 atgttgcaac gtacgctggg cagtggctgg ggagtgttgc tgccgggatt gctgattgca
     1801 gggctgatgt atgcggattt atcgccggat cagtggcgga ttgtcattct gatgggatta
     1861 gtattgacgc cggtgatgct gtatcacaaa cagttacggc attacgtttt gctaccatcg
     1921 tgcctggcgc ttattgctgc catcatgctg atgataatga atttgaatca gggatgaaaa
     1981 atcaaggaag aaacaagaaa ggaagtaaag ataattggtg cgaggggggg gacttgaacc
     2041 cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc
     2101 gcataccatc aattcttaaa aagaattgct accacggagg cgcattctag tggttttcag
     2161 cttttcgtca atagttaatt atcgccggag gtgtaattgc tggaaaaatg tccatcagga
     2221 aactagcgtg caggtttggt aggcatgcgg gggcagatgc cagatgcgac gctggcgcgt
     2281 cttatctggc ctacgaaggg ctaacgtgca ggttttgtag gtcggataag gcgttcacgc
     2341 cgcatccgac acggtattcg gcgagataat taacctttct tcgcctggcg ggtcataatg
     2401 gcgcgataca ccttgaaacg cccggtttgc gcaatcactt catggaagcc aaatgtctca
     2461 tccagcacgt ccgggtaagg caggaaggcg ttcgctacaa ttcgcagctc gccgccgcta
     2521 ttaagatgac gcaccgcgcc gcgaatcagc gtttgcgccg catccaggct ggtttgcatc
     2581 ccatcgtgga acggcgggtt ggagatgatc atatcaaaac gacctttcac ctcggaaaag
     2641 acgttgctgg caaagacttc accttcaaca cagttggccg caagtgttgc gcggctggct
     2701 tctaccgctg gcgcagagac atcgcacaag gtgagacgaa ttttcggcga atggcgcgca
     2761 aaggcaactg aaagcacccc cgcgccacag ccgacatcca gcactttacc tttcgtgtgc
     2821 ggagttaacg tcgagagcag caactggcta ccgacatcca gaccgtcgcg gctaaacacg
     2881 ccaggcagcg ttttgaccgt caggccatcg acgctgtatt cgccccagaa tttatcggca
     2941 tcaaataccg gctgtttttc cagacgacca aaatagaggc cacagcgacg agcgctgtcg
     3001 actttattca acggcgcata atctgccagc atctgctcgg cgctgcgcac gccgctgcgg
     3061 ttctcgccaa cgacaaaaat atctgtcccc actggcagca gagaaagtaa attcatcaac
     3121 tggaactggg cttccggttt gttcttcggc cagtagtaaa tcagcatatc gcaatctgcg
     3181 acgtcatcca ccgtggcgac cagactgaaa cgggcgttat cccccatctg gcggcttaat
     3241 acctgccagt ggtggaattg ctgggtatga gcacggctgg ccgcggtatc taaacgcgcg
     3301 ggcaggtcat cctgtaagtc tccggcaaac agaatacggc tttgttcgaa atcatcactg
     3361 tgacgcagca agacttcact tgccggggta aatgcagaca tggaatgctc ctcaattgat
     3421 actggcggcg attatagcca tatgttggcg cggtatcgac gaatttgcta tatttgcgcc
     3481 cctgacaaca ggagcgattc gctatgacat cccgacgaga ctggcagtta cagcaactgg
     3541 gcattaccca gtggtcgctg cgtcgccctg gcgcgttgca gggggagatt gccattgcga
     3601 tcccggcaca cgtccgtctg gtgatggtgg caaacgatct tcccgccctg accgatcctt
     3661 tagtgagcga tgttctgcgc gcattaaccg tcagccccga tcaagtgctg caactgacgc
     3721 cagaaaaaat cgcgatgctg ccgcaaggca gtcgctgcaa cagctggcgg ttgggtactg
     3781 acgaaccgct atcactggaa ggcgctcagg tggcatcacc ggcgctcacc gaattacggg
     3841 caaacccaac ggcacgcgcc gcgttatggc aacagatttg cacatatgaa cacgatttct
     3901 tccctcgaaa cgactgattt accagcggct taccacattg aacaacgcgc ccacgccttt
     3961 ccgtggagtg aaaaaacctt tgccagcaac cagggcgagc gttatctcaa ctttcagtta
     4021 acgcaaaacg gcaaaatggc ggcgtttgcg attacgcaag tggtgctgga tgaagctaca
     4081 ttgttcaata ttgcggtcga tcctgactat cagcgtcagg gattgggaag ggtgctgctg
     4141 gaacatctga tcgacgaact ggaaaaacgc ggcgtggcga cactatggct ggaagtccgt
     4201 gcctcaaatg ctgccgccat tgccctgtac gaaagtttag gctttaacga ggcgacgatt
     4261 cgccgcaatt actaccccac cacggacggt cgcgaagacg ccatcatcat ggcgttgcca
     4321 atcagtatgt aagacaaggt ggaataatga agtgggactg gattttcttt gatgccgatg
     4381 aaacgctgtt tacctttgac tcattcaccg gcctgcagcg gatgtttctt gattacagcg
     4441 tcacctttac cgctgaagat tttcaggact atcaggccgt taacaagcca ctgtgggtgg
     4501 attatcaaaa cggcgcgatc acttcattac agcttcagca cgggcgtttt gagagctggg
     4561 ccgaacggct gaaagttgaa gcaggcttgc ttaacgatgc ctttattaat gcgatggcgg
     4621 aaatctgcac gccgctgccg ggcgcggttt ctctgcttaa cgccattcgt ggcaacgcca
     4681 aaatcggcat catcaccaac ggctttagcg ccttgcagca agtgcgtctg gaacgcacgg
     4741 gcctgcgtga ttacttcgat ttgctggtga tttccgaaga agttggcgtt gccaaaccga
     4801 ataagaaaat tttcgattat gcgctggaac aggcgggcaa tcctgaccgt tcacgcgtgc
     4861 tgatggttgg cgacactgcc gagtccgata ttctcggtgg catcaacgcc gggcttgcga
     4921 cctgctggct gaatgcgcac aatcgcgagc aaccagaagg catcgcgccc acctggaccg
     4981 tttcttcgtt gcacgaactg gagcagctcc tgtgtaaaca ctgattgcct cccccccgtt
     5041 gatgggtaaa atagccgcaa tttttcgttt tcaacaagcg cggcgcgatg ccgcttactc
     5101 aagaagaaag aattatgacg ttgtctcctt atttgcaaga ggtggcgaag cgccgcactt
     5161 ttgccattat ttctcacccg gatgccggta agactaccat caccgagaag gtgctgctgt
     5221 tcggacaggc cattcagacc gccggtacag taaaaggccg tggttccaac cagcacgcta
     5281 agtcggactg gatggagatg gaaaagcagc gtgggatctc cattactacg tctgtgatgc
     5341 agtttccgta tcacgattgc ctggttaacc tgctcgacac cccggggcac gaagacttct
     5401 cggaagatac ctatcgtacc ctgacggcgg tggactgctg cctgatggtt atcgacgctg
     5461 caaaaggtgt tgaagatcgt acccgtaagc tgatggaagt tacccgtctg cgcgacacgc
     5521 cgatcctcac ctttatgaac aaacttgacc gtgatatccg cgacccgatg gaactgctcg
     5581 atgaagttga gaacgagctg aaaatcggct gcgcaccgat cacctggccg attggctgcg
     5641 gcaagctgtt taaaggcgtt taccaccttt ataaagacga aacctatctc tatcagagcg
     5701 gtaaaggcca cacgattcag gaagtccgta ttgttaaagg gctgaataac ccggatctcg
     5761 acgctgcggt tggtgaagat ctggcacagc agctacgtga cgaactggaa ctggtgaaag
     5821 gcgcgtctaa cgagttcgac aaagaattgt tccttgcggg cgaaatcact ccggtattct
     5881 tcggtactgc gctgggtaac ttcggcgttg atcatatgct ggatggcctg gtggagtggg
     5941 ctcctgcgcc gatgccgcgt cagactgata cccgtaccgt agaggcgagc gaagacaaat
     6001 ttaccggctt cgtatttaaa attcaggcca acatggaccc gaaacaccgc gaccgcgtgg
     6061 cgtttatgcg cgtagtatcc ggtaaatatg aaaaaggcat gaagctgcgc caggtgcgta
     6121 ctgcgaaaga tgtggttatc tccgacgcgc tgacctttat ggcgggcgac cgttcgcacg
     6181 ttgaagaagc gtatccgggc gatatcctcg gcctgcacaa ccacggcact attcagatcg
     6241 gcgacacctt tacccagggt gagatgatga agttcaccgg tattccgaac ttcgcaccag
     6301 aactgttccg tcgtatccgc ctgaaagatc cgctgaagca aaaacagctg ctcaaagggc
     6361 tggtacagct ttccgaagag ggcgcggtgc aggtgttccg tccgatctcc aacaacgatt
     6421 tgatcgttgg tgctgttggt gtgctgcagt ttgatgtggt ggtatcgcgc ctgaagagcg
     6481 aatacagcgt tgaagcagta tatgaatcag tcaacgttgc cactgcccgc tgggtagaat
     6541 gtgcggacgc gaagaaattc gaagagttca agcgtaagaa cgaaagccaa ctggcgcttg
     6601 atggcggcga taacctcgct tacatcgcta ccagcatggt caacctgcgc ctggcgcagg
     6661 aacgttatcc cgacgttcag ttccaccaga cccgcgagca ttaattcttg cctccagggc
     6721 gcggtagccg ctgcgccctg tcaatttccc ttccttatta gccgcttacg gaatgttctt
     6781 aaaacattca cttttgctta tgttttcgct gatatcccga gcggtttcaa aattgtgatc
     6841 tatatttaac aaagtaatga catttctgac ggcgttaaat accgttcaat gcgtagatat
     6901 cagtatctaa agccgtcgat tgtcattcta ccgatattaa taactgattc agaggctgta
     6961 atggtcgtta ttcatcaccc atcgcttttg tgatggcgac cattgacttc tgtagagggt
     7021 gaagtctctc cctattcagc aatgcaacct cgtgttgcca ggctcaaatt acgagcaaac
     7081 atacaggaat aaatcgatga ctatgacaag actgaagatt tcgaaaactc tgctggctgt
     7141 aatgttgacc tctgccgtcg cgaccggctc tgcctacgcg gaaaacaacg cacagactac
     7201 caatgaaagc gcagggcaaa aagtcgatag ctctatgaat aaagtcggta atttcatgga
     7261 tgacagcgcc atcaccgcga aagtgaaggc ggcgctggtg gatcatgaca acatcaagag
     7321 caccgatatc tctgtcaaaa ccgatcaaaa agtcgtgacc ctgagcggtt tcgttgaaag
     7381 ccaggctcag gccgaagagg cagtgaaagt ggcgaaaggc gttgaagggg tgacctctgt
     7441 cagcgacaaa ctgcacgttc gcgacgctaa agaaggctct gtgaagggct acgcgggtga
     7501 caccgccacc accagtgaaa tcaaagccaa actgctggcg gacgatatcg tcccttcccg
     7561 tcatgtgaaa gttgaaacca ccgacggcgt ggttcagctc tccggtaccg tcgattccca
     7621 ggcacagagc gaccgtgctg aaagtatcgc caaagcggta gatggtgtga aaagcgttaa
     7681 aaatgatctg aaaactaagt aattcgtcgt aattcgtcct cccgaacttt gtcgggaggc
     7741 gcgatgtgca ccacactaaa aatatcgcat tgagtgattt tcacgctcat atcaagcggt
     7801 gacattaact atggtaaagg agacgcttat gtttcgttgg ggcatcatat ttctggttat
     7861 cgcgttaatc gccgccgcac ttgggtttgg tggtctggcc ggtaccgctg caggcgcagc
     7921 taaaattgtc tttgtcgtcg ggattattct gttcctggtg agtttgttca tgggccgaaa
     7981 acgaccctag atttcagacc attaagaaaa ttttatccaa agccagtcca gcggactggc
     8041 ttttgcggtt ttagcgaata ataaattgcg ttactttgtc attctttcac aacaaaaaca
     8101 ggaaggcaga ggtggggcag cgaatacctg taacgcttgg taatattgcg ccgttgtcgc
     8161 taaggccgtt ccagcctgga cgaatagctc tggtgtgcga aggcggcgga cagcgtggaa
     8221 ttttcacggc tggcgtgctg gatgagttta tgcgcgcgca gtttaatcct ttcgatcttt
     8281 atctcggcac atctgccggg gcgcagaacc tctcggcgtt tatctgcaat cagcccggtt
     8341 acgcgcgcaa agtcatcatg cgctatacca caaaacgcga atttttcgat ccattgcgct
     8401 ttgtccgtgg aggaaatctt atcgatctcg actggctggt ggaggccacc gcaagccaga
     8461 tgccgttgca aatggatacc gccgcgcggt tgtttgacag cggcaaatcg ttttatatgt
     8521 gcgcctgtcg tcaggatgac tacgcgccta attacttttt accaaccaaa caaaactggc
     8581 tggatgtgat tcgcgcctcc agtgcgatac ctggctttta tcgtagcgga gtgtcgctgg
     8641 aaggcattaa ctacctggat ggcgggatca gtgatgcgat tccggttaaa gaggcggcaa
     8701 ggcagggcgc taaaacgttg gtcgtcattc gcactgtgcc gtcacaaatg tactacacgc
     8761 cgcagtggtt caaacgcatg gaacgctggc tgggtgacag tagcctgcag ccgctggtca
     8821 atctggtgca gcatcatgaa accagctatc gtgacattca gcaatttatt gagaaaccac
     8881 cgggcaagct gcggatattc gaaatttatc cgccgaagcc attacatagt atcgcgcttg
     8941 gcagtcggat tccggcgctg cgtgaagact ataaacttgg gcgtttatgc ggtcgttatt
     9001 tcctcgccac ggttggcaag ctattaactg aaaaagcgcc gcttacccgc catctggtgc
     9061 cagtggtgac gccggaatcg atcgttattc cgcctgcgcc agtcgccaac gatacgctgg
     9121 ttgccgaagt gagcgatgct ccgcaggcga acgacccgac atttaacaat gaggatctgg
     9181 cttgatttgc cgttttatcg atacccactg ccattttgat ttcccgccgt ttagtggcga
     9241 tgaagaggcc agcctgcaac gcgcggcaca agcgggcgta ggcaagatca ttgttccggc
     9301 gacagaggcg gaaaattttg cccgcgtgca ggcgttagcg gaaaaatatc aaccgctgta
     9361 tgccgcattg gggttgcatc cggggatgtt ggaaaaacat agcgatgtgt ctcttgatca
     9421 gctacagcag gcgctggaaa ggcgtccggc gaaggtggtg gcggtggggg agatcggtct
     9481 ggatctcttc ggcgacgatc cgcaatttga gaggcagcag tggttactcg acgaacagct
     9541 gaaactggcg aaacgctacg atctgccggt gatccttcat tcccggcgta tacacgacaa
     9601 actggcgatg catcttaaac gtcacgattt accgtgcact ggcgtggttc acggtttttc
     9661 cggcagcctg caacaggccg agcggtttgt acagctgggc tacaaaatcg gcgtaggcgg
     9721 tactatcacc tatccacgcg ccagtaaaac ccgcgatgtc atcgcaaaat taccgctggc
     9781 atcgttgttg ctggaaaccg acgcgccgga tatgccgctc aacggctttc aggggcagcc
     9841 taaccgaccg gaacaggctg tgcgggtgtt tgacgtgctt tgcgagttgc gcccggaacc
     9901 agaagatgag attgccgaag tgttgcttaa taatacgtat gcgttgttta gcgttagtgg
     9961 gtagcctacc cttcgtaggc agcatccggc agtcggcgca taatgcctga tgcgacgctt
    10021 aacgcgtctt atcatgccta caggtttgtg ccgaaccgta ggccggataa ggcgttcacg
    10081 ccgcatccgg cagttggcgc acaatgcctg atgcgacgct tgacgcgtct tatcatgcct
    10141 actggtttgt gccgaaccgt aggccggata aggcgttcac gccgcatccg gcagttggcg
    10201 cacaatgcct gatgcgacgc ttgacgcgtc ttatcatgcc tacaagtctg tgccgaaccg
    10261 taggccggat aaggcgttca cgccgcatcc ggcagttggc gcacaatgcc tgatgcgacg
    10321 ctgacgcgtc ttatcatgcc tacaagtctg tgccgaaccg taggccggat aaggcgttca
    10381 cgccgcatcc ggcagtcggc gcataatgcc tgatgcgacg cttgtcgcgt cttatcatgc
    10441 ctacaagtct gtgccgaacc gtaggccgta tccggcatgt cacaaataga gcgccggaaa
    10501 tatcaaccgg ctcaccccgc gcacctttaa cgcatcagcc aacggctcaa cgtcttccgg
    10561 cgtggcgctc gcccagcttt gcgcctcgcc atacacgccg tgggcatgaa acgcattcag
    10621 gcgaaccgga acatcgccaa gtctcttgat aaacgtcgcc agctcatcga tgtgatgcaa
    10681 ataatccacc tggtctggaa ttaccagcaa acgcagttcc gccagcttgc cgcgctctgc
    10741 aagcaaacag atgctgcgct taatctgctg attatcgcgt ccggttaagt gttgatgaca
    10801 ttcgctcccc cacgctttga gatcgagcat tgcgccgtca cacaccggga gtaatttttc
    10861 ccagccggtt tcgctcaaca tgccgttact gtccaccagg caggtgaggt ggcgcagttg
    10921 cggatcgttt ttgatagcag taaacagcgc cactacaaac ggcagttggg tcgtggcttc
    10981 gccgccgctt accgttatcc cttcaataaa cagcaccgct ttgcggacat ggctaagcac
    11041 ttcgtccacg ctcatggatt gcgccattgg cgtggcatgt tgcggacacc tcttcaggca
    11101 ggtatcacac tgctcgcaaa ccacagcgtt ccacaccact ttgccgtcaa caatctgcaa
    11161 cgcctgatgc ggacactgtg gcacgcactc cccacagtca ttgcaacgtc ccatcgtcca
    11221 cggattgtga cagtttttgc agcgcagatt gcagccctgc aaaaacagag ccagacgact
    11281 gcctggcccg tcaacgcagg agaaggggat aatcttactg actaaagcgc atctgctgtt
    11341 catggcttat cacgcgcggc tggcgttcca gaatacgagt gttgcgtgcg gcttcttcgc
    11401 ccagccaggt ggtgttggtg cgtgaacctt cggcgcgata tttttctaaa tccgacaaac
    11461 gcaccatata accggtaacg cgaaccagat cgttaccgct gacattggca gtaaattcac
    11521 gcattccggc tttaaaggca ccgaggcaaa gctgtaccag cgcctgcgga ttacgtttga
    11581 tggtttcgtc gagcgtcaga atgtcgctga tgccggaata ataataagca tgatgcgggg
    11641 cgacagtttg cagatgggtg atcggatctg gctcatcacc atacggcaaa cgcgcgcccg
    11701 gcgtggtgcc gatatcggaa ctgctccccg actgtgcgtg taacatggcg cgtttttgcc
    11761 agccatattt cacgggggta ttggcgacaa actccgccag ttgcgcgctg atgcgataac
    11821 ccacttcatt tgcggcggct tctttaccgt agcgcgcggc aatcccttct ttcttacaca
    11881 gcaagttaac cgcttccgcc agcccataca tgccaaacat tggcacaaaa cgttcagggt
    11941 taatcagccc ttctttcacc aggaagctat tctcaaagaa gtgtgattgt tgatagagga
    12001 attcacaccg cgcatcgatg atggcgatct gctgctggca gtagtgcggt agagtgcgcg
    12061 taaagaagtc atccagcgat tcgctgcgct cggcaatggc tttcaggtta aggcgtacca
    12121 gcgtgctgcc accacccgcc agcggcagtg agttgtaaca gctcacaatc ccgtagcccc
    12181 cttttgtgaa aattttatca tgcaccggac cgttggcgat gtgcggtttg ctacattcac
    12241 agatgttctt cgccacttcc agcagcaggt catcaggggt gatttcagga tcgtagataa
    12301 aggtcaggtt cggtgaaacc tgcttcaact ctgcgtctgc acgtaagatc gcgcgggtaa
    12361 tgggcgaatc agacgggccg atattggcgt gcataaaggc gtctggcagg gttctgtcga
    12421 ggtaacgcca gaaacgtttt attcgaacat cgatctcgtc ttgtgttaga attctaacat
    12481 atggttgcaa caacgcatcc agttgcccca ggtagaccgg catcgatgtg accgacggta
    12541 cgtggtggta aagaatggtc agcagagaga gtgcgtcatc aagatctttc gcgccttcca
    12601 gctccagcca ttcggaaccg ttcgccagaa aacgggcgta atcgggtaag acatagcgcg
    12661 gtttgtacgg cgcatgacct tcaaacatat cgcagattac accctcatcc agcgcgcggc
    12721 gggcttcggc aggaagctgc ggataaggca gattgttttc tgcttccagt gccagaaaat
    12781 ggcgcttctg ctccgggcta agcactgggc tggtgacaat ttgctggcaa cgttgttgca
    12841 gtgcattttc atgagaagtg ggcatcttct tttcctttta tgccgaaggt gatgcgccat
    12901 tgtaagaagt ttcgtgatgt tcactttgat cctgatgcgt ttgccaccac tgacgcattc
    12961 atttgaaagt gaattatttg aaccagatcg cattacagtg atgcaaactt gtaagtagat
    13021 ttccttaatt gtgatgtata tcgaagtgtg ttgcggagta gatgttagaa tactaacaaa
    13081 ctcgcaaggt gaattttatt ggcgacaagc caggagaatg aaatgactga tctgaaagca
    13141 agcagcctgc gtgcactgaa attgatggac ctgaccaccc tgaatgacga cgacaccgac
    13201 gagaaagtaa ttgctctgtg tcatcaggcc aaaaccccgg tcggcaatac cgccgctatc
    13261 tgtatctatc ctcgctttat cccgattgct cgcaaaacgc tgaaagagca gggcaccccg
    13321 gaaatccgaa tcgctacggt aaccaacttc ccacacggta acgatgacat cgaaatcgcg
    13381 ctggcagaaa cccgtgcggc aatcgcctac ggtgctgatg aagttgacgt tgtgttcccg
    13441 taccgcgcgc tgatggcggg taacgagcag gttggttttg acctggtgaa agcctgtaaa
    13501 gaggcttgcg cggcagcgaa tgtactgctg aaagtgatca tcgaaaccgg cgaactgaaa
    13561 gacgaagcgc tgatccgtaa agcgtctgaa atctccatca aagcgggtgc ggacttcatc
    13621 aaaacctcta ccggtaaagt ggctgtgaac gcgacgccgg aaagcgcgcg catcatgatg
    13681 gaagtgatcc gtgatatggg cgtagaaaaa accgttggtt tcaaaccagc gggcggcgtg
    13741 cgtactgcgg aagatgcgca gaaatatctc gccatcgcag atgaactgtt cggtgctgac
    13801 tgggcagatg cgcgtcacta ccgctttggt gcttccagcc tgctggcaag cctgttgaaa
    13861 gcgctgggcc acggtgacgg taagagcgcc agcagctact aagtaagatg ctttacgcct
    13921 gatgcgctgc gcttatcagg cctacgagac gtatctaccc gtaggccgga taaggcgtag
    13981 acgcatccgg caaaagccgc ctcatactct tttcctcggg aggttacctt gtttctcgca
    14041 caagaaatta ttcgtaaaaa acgtgatggt catgcactga gcgatgaaga aattcgtttc
    14101 tttatcaacg gcattcgcga caacactatc tccgaagggc agattgccgc cctcgcgatg
    14161 accattttct tccacgatat gacaatgcct gagcgtgtct cgctgaccat ggcgatgcga
    14221 gattcaggaa ccgttctcga ctggaaaagc ctgcatctga atggcccgat tgttgataaa
    14281 cattccactg gtggcgtcgg cgatgtgact tcgctgatgt tggggccgat ggtcgcagcc
    14341 tgcggcggct atattccgat gatctctggt cgcggcctcg gtcatactgg cggtacgctc
    14401 gacaaactgg aatccatccc tggcttcgac attttcccgg atgacaaccg tttccgcgaa
    14461 attattaaag acgtcggcgt ggcgattatc ggtcagacca gctcactggc tccggcggat
    14521 aaacgtttct acgcgacccg tgatattacc gcaaccgtgg actccatccc gctgatcacc
    14581 gcctctattc tggcgaagaa acttgcggaa ggtctggacg cgctggtgat ggacgtgaaa
    14641 gtgggtagcg gcgcgtttat gccgacctac gaactctctg aagcccttgc cgaagcgatt
    14701 gttggcgtgg ctaacggcgc tggcgtgcgc accaccgcgc tgctcaccga catgaatcag
    14761 gtactggcct ccagtgcagg taacgcggtt gaagttcgtg aagcggtgca gttcctgacg
    14821 ggtgagtatc gtaacccgcg tctgtttgat gtcacgatgg cgctgtgcgt ggagatgctt
    14881 atctccggca aactggcgaa agatgacgcc gaagcgcgcg cgaaattgca agcagtgctg
    14941 gacaacggta aagcggcaga agtctttggt cgtatggtag cggcacaaaa aggcccaacc
    15001 gacttcgttg agaactacgc gaagtatctg ccgacagcga tgctgacgaa agcagtctat
    15061 gctgataccg aaggttttgt cagtgaaatg gatacccgcg cgctggggat ggcagtggtt
    15121 gcaatgggcg gcggtcgtcg tcaggcatct gacaccattg attacagcgt cggctttact
    15181 gatatggcgc gtttgggcga ccaggtagac ggtcagcgtc cgctggcggt tatccacgcg
    15241 aaagacgaaa acagctggca ggaagcggcg aaagcggtga aagcggcaat taaacttgcc
    15301 gataaagcac cggaaagcac accaactgtc tatcgccgta tcagcgaata acggtatact
    15361 gatctgatca tttaaatttg aagcactgag tacggagaac atatgaaacg tgcatttatt
    15421 atggtgctgg actcattcgg catcggcgct acagaagatg cagaacgctt tggtgacgtc
    15481 ggggctgaca ccctgggtca tatcgcagaa gcttgtgcca aaggcgaagc tgataacggt
    15541 cgtaaaggtc cgctcaatct gccaaatctg acccgtctgg ggctggcgaa agcacacgaa
    15601 ggttctaccg gtttcattcc ggcgggaatg gacggcaacg ctgaagttat cggcgcgtac
    15661 gcatgggcgc acgaaatgtc atccggtaaa gataccccgt ctggtcactg ggaaatagcc
    15721 ggtgtcccgg ttctatttga gtggggatat ttctccgatc acgaaaacag cttcccgcaa
    15781 gagctgctgg ataaactggt tgaacgcgct aatctgccgg gttacctcgg taactgccac
    15841 tcttccggta cggtcattct ggatcagctg ggcgaagagc acatgaaaac cggcaagccg
    15901 attttctata cctccgctga ctctgtgttc cagattgcct gccacgaaga aacctttggc
    15961 ctggataaac tctacgaact gtgcgaaatc gcccgtgaag agctgaccaa cggcggctac
    16021 aacatcggtc gtgttatcgc tcgtccgttt atcggtgaca aagccggtaa cttccagcgt
    16081 accggtaacc gtcacgacct ggctgttgag ccgccagcac cgaccgtgct gcagaaactg
    16141 gttgatgaaa aacacggtca ggtggtttct gtcggtaaaa ttgcagacat ctacgccaac
    16201 tgcggtatca ccaagaaagt gaaagcaact ggcctggacg cgctgtttga cgccaccatc
    16261 aaagagatga aagaagcggg tgataacacc atcgtcttca ccaacttcgt tgacttcgac
    16321 tcttcctggg gccaccgtcg cgacgtcgcc ggttatgctg tgggtctgga gctgttcgac
    16381 cgccgtctgc cggagctgat gtctctgctg cgcgatgacg acatcctgat cctcactgct
    16441 gaccacggtt gtgatccgac ctggaccggt actgaccaca cgcgtgaaca cattccggta
    16501 ctggtttacg gcccgaaagt aaaaccgggc tcactgggtc accgtgaaac cttcgcggat
    16561 atcggccaga ctctggcaaa atattttggt acttctgata tggaatatgg caaagccatg
    16621 ttctgataat gggtgcggct tgatgccctc accccggccc tctccctcag ggagagggtg
    16681 aaaacatgtt gataaggata aaacaatggc taccccacac attaatgcag aaatgggcga
    16741 tttcgctgac gtagttttga tgccaggcga cccgctgcgt gcgaagtata ttgctgaaac
    16801 tttccttgaa gatgcccgtg aagtgaacaa cgttcgcggt atgctgggct tcaccggtac
    16861 ttacaaaggc cgcaaaattt ccgtaatggg tcacggtatg ggtatcccgt cctgctccat
    16921 ctacaccaaa gaactgatca ccgatttcgg cgtgaagaaa attatccgcg tgggttcctg
    16981 tggcgcagtt ctgccgcacg taaaactgcg cgacgtcgtt atcggtatgg gtgcctgcac
    17041 cgattccaaa gttaaccgca tccgttttaa agatcatgac tttgccgcta tcgctgactt
    17101 tgacatggtg cgtaacgcgg tagacgcggc taaagcactg ggcattgatg ctcgcgttgg
    17161 taacctgttc tccgctgacc tgttctactc tccggacggc gaaatgttcg acgtgatgga
    17221 aaaatacggc atcctcggcg tggaaatgga agcggctggt atctacggcg tcgctgcaga
    17281 atttggcgcg aaagccctga ccatctgcac tgtgtctgac cacatccgca ctcacgagca
    17341 gaccactgcc gctgagcgtc agaccacctt caacgacatg atcaaaatcg cactggaatc
    17401 cgttctgctg ggcgataaag agtaattgtg tttcgctgaa aggcgattgt cgtgttaagc
    17461 cggagtgggg gactgctccg gctttttag
//
LOCUS       DAZPJR010000078        17448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPJR010000078 DAZPJR010000000
VERSION     DAZPJR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.079"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..502)
                     /locus_tag="ACHVB7_002453"
     CDS             complement(<1..502)
                     /locus_tag="ACHVB7_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1914416.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="ACHVB7_002454"
     tRNA            complement(688..772)
                     /locus_tag="ACHVB7_002454"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="ACHVB7_002455"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="ACHVB7_002455"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR1914417.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="ACHVB7_002456"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="ACHVB7_002456"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1914418.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="ACHVB7_002457"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="ACHVB7_002457"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR1914419.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="ACHVB7_002458"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="ACHVB7_002458"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR1914420.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="ACHVB7_002459"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="ACHVB7_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR1914421.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="ACHVB7_002460"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="ACHVB7_002460"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR1914422.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="ACHVB7_002461"
     CDS             7117..8619
                     /locus_tag="ACHVB7_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR1914423.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="ACHVB7_002462"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="ACHVB7_002462"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1914424.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="ACHVB7_002463"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="ACHVB7_002463"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1914425.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10983..11033)
                     /gene="ytgA"
                     /locus_tag="ACHVB7_002464"
     CDS             complement(10983..11033)
                     /gene="ytgA"
                     /locus_tag="ACHVB7_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR1914426.1"
                     /translation="MSIIILATISTTQENK"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="ACHVB7_002465"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="ACHVB7_002465"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR1914427.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="ACHVB7_002466"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="ACHVB7_002466"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR1914428.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="ACHVB7_002467"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="ACHVB7_002467"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR1914429.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="ACHVB7_002468"
     CDS             complement(16186..17382)
                     /locus_tag="ACHVB7_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR1914430.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDATYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4337 c         4453 g         4175 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagcatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAZPJR010000079        17306 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPJR010000079 DAZPJR010000000
VERSION     DAZPJR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.080"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHVB7_002469"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHVB7_002469"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR1914431.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHVB7_002470"
     CDS             complement(1672..2667)
                     /locus_tag="ACHVB7_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1914432.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHVB7_002471"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHVB7_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHVB7_002472"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHVB7_002472"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR1914433.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHVB7_002473"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHVB7_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR1914434.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHVB7_002474"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHVB7_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR1914435.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHVB7_002475"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHVB7_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR1914436.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHVB7_002476"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHVB7_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR1914437.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHVB7_002477"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHVB7_002477"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR1914438.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHVB7_002478"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHVB7_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR1914439.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHVB7_002479"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHVB7_002479"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR1914440.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHVB7_002480"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHVB7_002480"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR1914441.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         4984 a         3997 c         3725 g         4600 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattt tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caagagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataaggctc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgt
//
LOCUS       DAZPJR010000080        17270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPJR010000080 DAZPJR010000000
VERSION     DAZPJR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17270
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.081"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..318)
                     /locus_tag="ACHVB7_002481"
     CDS             complement(<1..318)
                     /locus_tag="ACHVB7_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914442.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTF"
     gene            718..1701
                     /locus_tag="ACHVB7_002482"
                     /pseudo
     CDS             718..1701
                     /locus_tag="ACHVB7_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(2017..3219)
                     /locus_tag="ACHVB7_002483"
     CDS             complement(2017..3219)
                     /locus_tag="ACHVB7_002483"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR1914443.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3451..4950
                     /gene="pitA"
                     /locus_tag="ACHVB7_002484"
     CDS             3451..4950
                     /gene="pitA"
                     /locus_tag="ACHVB7_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR1914444.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(5194..5529)
                     /gene="uspB"
                     /locus_tag="ACHVB7_002485"
     CDS             complement(5194..5529)
                     /gene="uspB"
                     /locus_tag="ACHVB7_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR1914445.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5920..6354
                     /gene="uspA"
                     /locus_tag="ACHVB7_002486"
     CDS             5920..6354
                     /gene="uspA"
                     /locus_tag="ACHVB7_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR1914446.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(6453..6506)
                     /locus_tag="ACHVB7_002487"
     CDS             complement(6453..6506)
                     /locus_tag="ACHVB7_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914447.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6672..8141
                     /gene="dtpB"
                     /locus_tag="ACHVB7_002488"
     CDS             6672..8141
                     /gene="dtpB"
                     /locus_tag="ACHVB7_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR1914448.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(8190..8942)
                     /gene="rsmJ"
                     /locus_tag="ACHVB7_002489"
     CDS             complement(8190..8942)
                     /gene="rsmJ"
                     /locus_tag="ACHVB7_002489"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR1914449.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8950..10992)
                     /gene="prlC"
                     /locus_tag="ACHVB7_002490"
     CDS             complement(8950..10992)
                     /gene="prlC"
                     /locus_tag="ACHVB7_002490"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR1914450.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            11195..12037
                     /gene="rlmJ"
                     /locus_tag="ACHVB7_002491"
     CDS             11195..12037
                     /gene="rlmJ"
                     /locus_tag="ACHVB7_002491"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR1914451.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            12109..13461
                     /gene="gorA"
                     /locus_tag="ACHVB7_002492"
     CDS             12109..13461
                     /gene="gorA"
                     /locus_tag="ACHVB7_002492"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR1914452.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13515..13598)
                     /gene="dinQ"
                     /locus_tag="ACHVB7_002493"
     CDS             complement(13515..13598)
                     /gene="dinQ"
                     /locus_tag="ACHVB7_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1914453.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13626..13799
                     /locus_tag="ACHVB7_002494"
     CDS             13626..13799
                     /locus_tag="ACHVB7_002494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914454.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            14338..14691
                     /gene="arsR"
                     /locus_tag="ACHVB7_002495"
     CDS             14338..14691
                     /gene="arsR"
                     /locus_tag="ACHVB7_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR1914455.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14745..16034
                     /gene="arsB"
                     /locus_tag="ACHVB7_002496"
     CDS             14745..16034
                     /gene="arsB"
                     /locus_tag="ACHVB7_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR1914456.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            16047..16472
                     /gene="arsC"
                     /locus_tag="ACHVB7_002497"
     CDS             16047..16472
                     /gene="arsC"
                     /locus_tag="ACHVB7_002497"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR1914457.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16606..16902)
                     /locus_tag="ACHVB7_002498"
     CDS             complement(16606..16902)
                     /locus_tag="ACHVB7_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1914458.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            17009..>17270
                     /locus_tag="ACHVB7_002499"
     CDS             17009..>17270
                     /locus_tag="ACHVB7_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1914459.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT         4026 a         4441 c         4337 g         4466 t
ORIGIN      
        1 aaacgtgtta gtagtattat cggcgaaact attatattct tgaatatcta tagatggctc
       61 actattgtaa aaccatattt tctccatctg aagactatgt tcatttaccc tccagtgacc
      121 atgttgcagg aaatagatga tattcaggcc taaatcgtta tctatttgcg aaaagtcatt
      181 cagcatatta acttcaactg tgtttgatcg atatttttta tcaaccttgc catggtgttg
      241 atgactgtta actttatctg aatgcttatc ttgatggttt gaagagaagt gcttaaatat
      301 attgtgaaat actttcataa ataacctgta atatttttac ttgttagtaa caaaataaac
      361 aatacatgtc tgaaagacat atttacgatg atacaaagca ataatactta tatgttcgat
      421 gattttaagc accgagacta tccggtcttt tactaaataa tggaaaacaa tccatccctg
      481 gaagataatt gtaaaaaaaa caacagaata agaattaatc ttaggataaa tatttattta
      541 tcatggcctt ttaaccggta aaaaatggca ctcggcacag taaaccccgc tatagttatg
      601 ctatgcattt gtctttaatg atatctcgtc gttatattaa caggctgaaa ttatcattaa
      661 tgcacatttc attattagca gggatacctg aggagtttaa agtgaacata tatatcgggt
      721 ggctttgtgc tggaaagttc agggcaaagc gagtatttcg tcgcgggtca tgtgctgatt
      781 tctctggcgg ccatatgcct ggcattattc actaccgcat ttattatcat ttcgcagctc
      841 acgcgcggcg ttaatacgtt ttacaataca ttgttcccca ttattggcta tgcggggtca
      901 attatcacca tgatatgggg ttgggcactg ttagcaggca atgatgtgat ggcagacgag
      961 tttgtcgccg gccatgttat tttcggcgtt ggtatgattg ccgcctgtgt atcgacggtg
     1021 gcagcgtcat ccggtcactt tctgctcatt cccaaaaatg cagcggggag caagagcgat
     1081 ggaacgccgg tacaggctta ttcttcatta atcggtaatt gcctcattgc cgttcccgtt
     1141 ttactcaccc tgctcggttt catttggtct attacgctgt tacgtagtgc tgacataact
     1201 ccgcattatg tcgcgggtca cgtattgctt gggttaaccg caatctgtgc ctgtctaatt
     1261 ggccttgttg ccacaattgt ccatcaaaca cgtaatacgt tttcaactaa agaacactgg
     1321 ctgtggtgtt attgggttat ttttctcggc tcaatcacgg tactgcaggg gatatacgtc
     1381 ttagtcagtt ccgatgcaag cgcccgactg gctcccggca ttattcttat ttgcctcgga
     1441 atgatctgtt acagcatatt ctcaaaagtc tggctactgg cactggtctg gagacgtacc
     1501 tgttcgttag ccaacagaat accgatgata cccgtcttca cctgcctgtt ttgccttttc
     1561 ctggcatcat ttcttgcgga aatggcgcag accgacatgg gatattttat tccttcgcga
     1621 gttctggtcg gtttgggagc ggtatgcttt acgttgttct caatcgtttc aatattagaa
     1681 gcgggttctg ctaaaaaata attgcaacgt accggataaa accagcgttg accatttgcg
     1741 taacgctggt ttttcttagg catcatgaaa taacgcagca ttaatgcata gtggttaagg
     1801 ataaaaaaac aacaaagtta ctgtcttttt cagcctgttc atattttata aagatctggc
     1861 cactgggtga gttttcaatc acctttcctt ccaccttata ttaagcctgg agggtttcag
     1921 ttcacgggct cattagaaaa taatcacaag atagccacta tcaaaattag ctcattttta
     1981 atgcggctct aataattttc atctttaggt aacaggtcag gacgacttcg ctgcaatcaa
     2041 atcctgcgca caagcccacg cactcgacca cgcccactgg aagttatagc cccccagcca
     2101 gccggtaacg tccatcactt cgccgatgaa gtacagccca ggcactttgc gcgcttccat
     2161 cgtccgtgaa gagagttcgt tggtgtccac gccgccgagc gtcacttcgg cagtgcgata
     2221 gccttcagtg ccgttgggtt gtacacgcca gtcggtcaat gtgctaatca gtgcctgttg
     2281 gtcacgcacg ttgagctgtt ttagcgaaac attcgggatt tgcccgagtt gctgtaagcg
     2341 ttcaaccaac cgcttcggta gatgaaccgc cagtgtgttt ttcaggctct gattcggatg
     2401 tgcgttacgc tgctcattca ggaaggtttc gagatccaca tccggtagca gattgatact
     2461 gacaaattca cccggttgcc agtagctgga aatctgcaac accgccggtc cagacaagcc
     2521 gcgatgggtg aagagtaagt tctcacggaa aacgatgccg ttttcagcgg taatcacgga
     2581 aggcaccgcc acgcccgcca gcacctgtaa ctcttcgagc aacggtttat gcagagtgaa
     2641 tggcaccaga cccgcacggg tcggcagcac gttgaggcca aattgttcgg caatcttata
     2701 accaaacggc gacgcgccca gccccggcat tgacagccca ccggtcgcca tgaccagctt
     2761 ttcgcaaccg acagtcatgc cgttcagatc aagcgtgaag cctgtttcat cctccgccac
     2821 actcagcact tcgctacgca atctgaaggt cacattgccc ttctcgcact catccaccag
     2881 catgtcgaca atctgctgcg cggagtcatc gcagaagagt tgccctaacg ttttctcgtg
     2941 ccatgcgatg ccgtgtttat tgaccagatc aatgaaatcc cactgggtaa aacgtgcgag
     3001 tgcagactta caaaaatgcg gattctggct cagataagcg cctggttcga cataaaggtt
     3061 ggtaaagttg cagcgcccac cgccagacat aaggattttg cgccctggtt ttttaccatt
     3121 atcgatcagc agaacccggc gtcctgcctg acctgccagc gcagaacaga acatacccgc
     3181 cgcaccagcg cctataataa tggcatcaaa cctttccacg ttgcgctcct cttagaaaaa
     3241 acgggcgtga attgtaaaga ttcctcagtg gtcgcaccag catcaatatt actaaaagga
     3301 agtatttgcc tgaattatat aagataatta ttttttgagt gaaatccata caggaggcaa
     3361 atcaaaaaaa gtctatattt cactttgccc gcgccgcgaa agtcactgat aatgcgccgc
     3421 gttcatgtcc tcaaaatggc gtaacgtcct atgctacatt tgtttgctgg cctggatttg
     3481 cataccgggc tgttattatt gcttgcactg gcttttgtgc tgttctacga agccatcaat
     3541 ggtttccatg acacagccaa cgccgtggca accgttatct atacccgcgc gatgcgttct
     3601 cagctcgccg tggttatggc ggcggtgttc aactttttgg gtgttttgct gggtggtctg
     3661 agtgttgcct atgccattgt gcatatgctg ccgacggatc tgctgcttaa tatgggatcg
     3721 tctcatggcc ttgccatggt gttctctatg ttgctggcgg cgattatctg gaacctgggt
     3781 acctggtact ttggtttacc tgcatccagc tctcatacgc tgattggcgc gatcatcggg
     3841 attggtttaa ccaatgcgtt gatgaccggg acgtcagtgg tggatgcact caatatcccg
     3901 aaagtattaa gtattttcgg ttctctgatc gtttccccta ttgtcggcct ggtgtttgct
     3961 ggcggtctga ttttcttgct gcgtcgctac tggagcggca ccaagaaacg cgcccgtatc
     4021 cacctgaccc cagcggagcg tgaaaagaaa gacggcaaga aaaagccgcc gttctggaca
     4081 cgtatcgcgc tgatcctttc cgctatcggc gtggcgtttt cgcacggcgc gaacgatggt
     4141 cagaaaggca ttggtctggt tatgttggta ttgattggcg tcgcgccagc aggcttcgtg
     4201 gtgaacatga atgccactgg ctacgaaatc acccgtaccc gtgatgccat caacaacgtc
     4261 gaagcttact ttgagcagca tcctgcgctg ctcaaacagg ctaccggtgc tgatcagtta
     4321 gtaccggctc cggaagctgg cgcaacgcaa cctgcggagt tccactgcca tccgtcgaat
     4381 accattaacg cgctcaaccg cctgaaaggt atgttgacca ccgatgtgga aagctacgac
     4441 aagctgtcgc ttgatcaacg tagccagatg cgccgcatta tgctgtgcgt ttctgacact
     4501 atcgacaaag tggtgaagat gcctggcgtg agtgctgacg atcagcgcct gttgaagaaa
     4561 ctgaagtccg acatgcttag caccatcgag tatgcaccgg tgtggatcat catggcggtc
     4621 gcgctggcgt taggtatcgg tacgatgatt ggctggcgcc gtgtggcaac gactatcggt
     4681 gagaaaatcg gtaagaaagg catgacctac gctcagggga tgtctgccca gatgacggcg
     4741 gcagtgtcta tcggcctggc gagttatacc gggatgccgg tttccactac tcacgtactc
     4801 tcctcttctg tcgcggggac gatggtggta gatggcggcg gcttacagcg taaaaccgta
     4861 accagtattc tgatggcctg ggtgtttacc cttccggctg cggtactgct ttccggcggg
     4921 ctgtactggc tctccttgca gttcctgtaa tcgtacgcac caaaacgagc gggtcagctg
     4981 gcccgcttca gattgtgaca tagtgcgctt tgttcatgcc ggatgcggcg tgaacgcctt
     5041 atccggccta caaaaacatg caaattcaat agattgcaga gattatgtag gcctgataag
     5101 cgtagcgcat caggcaattt tgcgtttgta atcagtctcg agcgggtcag tttactggcc
     5161 cgcttttttt atgcgttatt attgctgaag tgcttaatgc caaatcatca atgcaatcag
     5221 gctgaccacc accagaccac acaatgcgct ggtcagaata aactgccgac gcacccgctc
     5281 acagcggcga ataaattcat catcgtgatg atcgcgataa cgttgggcat agatatacca
     5341 aacgagacgc acctgtttgt tgggttggcc atgtgaggta aaaaagcccc ctccatcaac
     5401 atattgatag agcaatggat cgcagttacg cagtaccact aacaacgcgc gtagtgatga
     5461 gaaatagcgc gccatgttaa caatgcaaac gacacataaa gcccaaaata atgcgacggt
     5521 gcttatcata cctcctcccc ggcgacctgc ccgcggagtt ccaccccggg gctaccgctc
     5581 ccgatacgct gccaatcagt taacaccagg tcctggagaa accgcttttg tggtgaccaa
     5641 catccgagcg gctctataga tagtgtagga gatcaggttg ttttttttcc agaaggttaa
     5701 ccactatcaa tatattcatg tcgaaaattt gtttatctaa cgggtaagca aggcggattg
     5761 acggatcatc cgggtcgcta taaggtaagg atggtcttaa cactgaatct ttacggctgg
     5821 gttagccccg cgcacgtagt tcgcaggacg cgggtgacgt aacggcacaa gaaacgctag
     5881 ctggccagtc atcgacaact ttatggaagg agtaacacta tggcttataa acacattctc
     5941 atcgcggtcg atctctcccc ggaaagcaaa gttctggtag agaaagcagt ctctatggct
     6001 cgcccctaca atgcgaaagt ttctctgatc cacgtagatg taaactactc tgacctatac
     6061 accgggctta ttgatgtgaa tctgggtgat atgcagaaac gcatctctga agagacacat
     6121 catgcgctga ccgagctttc cactaatgca ggctacccaa tcactgaaac cctgagcggc
     6181 agcggcgacc tgggccaggt tctggtcgat gcaatcaaga aatacgatat ggatctggtg
     6241 gtttgtggtc accaccagga cttctggagc aaactaatgt cttccgcacg tcagctgatc
     6301 aacaccgttc acgttgatat gctgattgtt ccgctgcgcg acgaagaaga ataatcatcc
     6361 ccccacgacg tgttcctgaa cgcccgcata tgcgggcgtt ttgctttttg gcgcgccttg
     6421 ttacctgatg cagcgtaaac cccttatctg gcctacgatc tgcgtacgca atcaaaatct
     6481 cctgccaata caacatttaa cactatcata ttttccatca ttagtgtgat cagctggtta
     6541 ttttctgttg taatagtgta ttaatctatt caccgcatca atattaagaa tctctgacag
     6601 atgtaaactt tttcgcgcgt tatcccttac gcgttcatac ttttcaggat ggtattggaa
     6661 ggttaataaa tatgaataca acaacaccca tggggatgct gcagcaacct cgcccatttt
     6721 tcatgatctt ttttgtcgag ttatgggagc gattcggcta ctacggcgtg cagggcgtac
     6781 tggcggtttt cttcgttaaa cagcttggat tctcgcaaga gcaggctttt gtcacttttg
     6841 gtgcttttgc tgcgctggtc tatggcctca tttccattgg cggctatgtc ggcgaccacc
     6901 tgctggggac caaacgcacc attgttctcg gagcacttgt gctggcgatt ggctacttca
     6961 tgaccggcat gtcgctactt aagcctgacc tgattttcat cgccctgggg actatcgctg
     7021 tcggtaacgg cctgtttaaa gctaacccag ccagcttgct ttcgaagtgc tatccgccga
     7081 aagatccgcg gcttgatggc gcattcaccc tgttctatat gtcgatcaat atcggctcgt
     7141 tgatagcgtt atcgctggcc cctgtgatcg ctgatagata cggttattca gtcacctaca
     7201 acctgtgcgg ggcggggtta attatcgcat tactggttta catcgcctgt cgtggaatgg
     7261 tgaaagacat tggttctgaa cccgacttca agccgatgag cttcagcaaa ctgttgtacg
     7321 tattacttgg cagcgtggtg atgatcttcg tatgtgcatg gctgatgcac aacgtagaag
     7381 tcgccaatct ggtgctgatt gttctctcca tcgtcgtcac catcatcttc tttcgtcagg
     7441 cattcaagct ggataaaacc gggcgcaata aaatgtttgt cgcctttgtc ctgatgctcg
     7501 aagcggtggt gttttacatt ctctacgccc agatgccaac gtcgctgaac ttctttgcca
     7561 tcaacaacgt gcatcatgaa attctcggct tttccataaa cccggtcagc ttccaggcgc
     7621 ttaacccgtt ctgggtggta ctcgccagcc caatactggc aggcatttac acgcatctgg
     7681 gtaacaaagg caaagacctc tcgatgccga tgaaatttac tctcggcatg tttatgtgct
     7741 cactgggctt tttgacggcg gcagctgcgg gaatgtggtt tgcggatgca caagggctga
     7801 catcgccatg gtttatcgtg ctggtgtatt tattccagag cctgggtgaa ttgtttatta
     7861 gcgcccttgg cctggcgatg attgctgccc tggtgccgca gcatttgatg ggctttattc
     7921 tcgggatgtg gttcctgacg caggctgccg cattcttgct gggcggctat gtggcaacat
     7981 ttaccgcagt gccggacaac attaccgatc cgcttgagac gttacccgtc tataccaacg
     8041 tgtttggtaa gattggcctg gttacgctgg gcgttgcagt agtgatgctg ttgatggtgc
     8101 cgtggctgaa acgcatgatt gcgacgccgg aaagccatta attattcttg cagaaagcag
     8161 ggtagcgtta tcgctaccct gctttacttt tacacgggcg tacctgcata gatatcaaag
     8221 cgatgccctt tagtcacaac cgcgtttggc gtggcgacat tcgctagcgg tggcgcgtag
     8281 tccgggcgct ttactaccac gcgtttggtc gccagcaagc gtgcaggctc cagcaatcca
     8341 tcggcatcaa gatccggccc caccagcgac tgaaagacac gcatctcttt tttcaccagc
     8401 gcgcttttct gcttatgcgg gaacatcggg tcgagataga ccacctgcgg gcgtggggta
     8461 atatcggtta gcgccgtcag gctggaggcg tgaattaact gcaaacgctc ctgcaaccag
     8521 ccgccgattt ccgcatccgc ataaccacgc gccaggccgt cgtcgagcag cgcggcaaca
     8581 actggattac gctccagcat ccgcacgcgg caaccgactg aagccagcac aaaggcatcg
     8641 cgccccagtc ctgcagtggc atccaccaca tccggcaaat aatcgccttt aatgcccacc
     8701 gctttcgcca ccgcctcacc gcgaccaccg ccgaatttgc gtcggtgcgc catcgctccg
     8761 ccaacaaaat caacaaagat gccgccaagt tttggctcat cacgcttgcg caattccaga
     8821 tgttccggcg ttaacaccag cgccatcagg ttgtcttcat cgtgctccag cccccagcgg
     8881 gccgccagaa cagataaggc gccgtctccg gcgcctgttt catcaattaa gcagattttc
     8941 actgaatgat cagcccttaa tgccgtaatg ctccagcatc gcatccagct gcggttcacg
     9001 accacggaag cgtttgaaca gatccatcgg ctcttctgaa ccgccacggc tcagaatgtt
     9061 gtcgaggaac gactgcccgg tttcacggtt gaaaatgccc tcttcctcaa agcgcgagaa
     9121 agcatctgcc gccagtacgt cagcccacag gtagctgtag taacctgccg cataaccacc
     9181 ggcgaaaata tggctgaaag cgtgcgggaa acggccccag gacggagatg gcaccacggc
     9241 aaccagtttc ttgatttctg ccagagtttc gaggattttt gccccctgat ccgggcggaa
     9301 ctcggcatga aggcggaaat caaacaggcc gaactccagc tgacgcagaa taaacagcgc
     9361 cgcctggtag ttcttcgccg ccagcatttt atccagcaac tctttcggca gcggttcgcc
     9421 ggtttcatag tgaccagaga taaacgccag cgcctccggc tcccagcacc agttttccat
     9481 aaactgactc ggcagttcga ccgcatccca cggcacaccg ctgataccgg aaacaccagc
     9541 ggtttcgatg cgggtcagca tatggtgcag gccgtgaccg aactcgtgga acagggtgat
     9601 cacttcgtcg tgagtgaaca gcgccggttt accatttacc gggcggttga agttacaagt
     9661 caaatacgcg accggttttt gcagagaacc atcagcttta cgcatctggc ctacgcagtc
     9721 atccatccac gccccgccgc gcttgttttc acgggcatac agatcgaggt agaagctgcc
     9781 gcgcagttcg ttattttcgt catacagttc gaagaaacgt acatccggat gccagacatc
     9841 aacctcttta cgctctttag cggtgatgcc gtaaatacgt ttcaccactt caaacaggcc
     9901 gttaaccgat ttgttttccg ggaagtacgg acgcagctgc tcgtcgctga tgctgtagag
     9961 gtgctgtttc tgtttttcgc tgtagtaagc gatatcccac ggctgcaact catcgacacc
    10021 aaattcggct ttagcgaagg cacgcagttg ctccagctct ttttcgcctt gcggacgcgc
    10081 gcgttttgcc agatcggtta agaaatccag cacctgctgc gggttttctg ccattttagt
    10141 ggcaagggat ttaaaggcgt agttttcaaa gcccagcagt tgcgccagtt cgtgacgcag
    10201 cgcgaggatc tcttccatca ccttgctgtt atcccactta ccggcgttcg ggccttgatc
    10261 ggaggcgcgg gtgctgtaag cgcgatacat ctcttcgcgc agagcctggt tgtcgcagta
    10321 ggtcattacc ggcaggtagc ttgggatatc cagcgtcagc aggtagcctt ccagctcttt
    10381 cgcttcggcc tgggcttttg ccgcagccag cgcgctttct ggcatccccg ccagccccgc
    10441 ttcgtcggta acgagtttgg tccagcccat cgtcgcatcg aggacgttgt tgctgtactg
    10501 gttgcccagt tcagaaagac gggtagcaat ttcgccgtaa cgctgctgtt tctctttcgg
    10561 cagacctatg ccagagagtt caaaatcacg cagggcgtta tcaaccgctt tcttctgcgc
    10621 cgtgttcagc gtggcgtaat gatcgccatc gcgcaggtcg cggtacgctt tatacagccc
    10681 ttcatgttgc cctacccagg tgctgtattc cgacagcagc ggcagggttt gctcgtaggc
    10741 ttcacgcagt tccgggctat ttttcaccga gttcaggtgg ctgaccgggg agaagatacg
    10801 ccccaacaca tcgtccactt ccgccaacgg ctggcagaga ttttcccagg tgtacggtgc
    10861 cccttgcgct actacgcgcg ccacattttc gcggcaatcg ttcagcgcct gagtcacggc
    10921 tggaacgaca tgttccggga gaattttaga aaacggaggc aattcaaagg gagtcagtaa
    10981 cggattcgtc attcgcgcag tcctggttaa agaggttaag gaagcgctca acaggcgctt
    11041 tacataatgt gtgtagcatg gggttaagtg tagtgaattt caatgagaaa cgttacgctt
    11101 tcgcggcggc ggcctctttt cggtatactg tcctgatacg cttttgtgcg ccccgaatac
    11161 gggccgattt ttacttaccg gaacaccttt acccatgctc agttatcgcc acagctttca
    11221 cgctggcaac cacgccgacg tccttaaaca taccgttcag agcctgatca tcgagtcgct
    11281 gaaagagaaa gataaaccgt ttctctatct cgatacccac gcaggggccg ggcgttatca
    11341 gttaggcagc gaacatgccg agcgtaccgg cgaatatctc gaaggtatcg cccgtatctg
    11401 gcagcaggac gatttgcccg cagaactgga ggcgtacatc aatgtggtaa aacacttcaa
    11461 ccgtagcggt cagttgcgtt actaccccgg gtcgccgttg attgctcgcc agctactgcg
    11521 cgaacaagac agcctgcaac tgaccgaact gcacccgagc gattacccgt tgctgcgttc
    11581 tgaatttcag aaagacagcc gtgcgcgcgt cgaaaaagcc gacggtttcc agcagcttaa
    11641 ggccaaactt ccgccggttt cccgccgtgg tttaatcctt atcgacccgc cgtatgaaat
    11701 gaaaaccgac tatcaggcgg tggtcagcgg gatagcagaa ggttacaaac gtttcgccac
    11761 tggtacttac gcactgtggt atccggtggt gctgcgtcag caaattaagc gcatgatcca
    11821 cgatctggaa gcgaccggta ttcgcaaaat tctgcaaatt gaactggcgg tactgccaga
    11881 cagcgatcgc cgtggcatga ccgcttccgg catgattgtg attaacccgc cgtggaaact
    11941 ggaacagcag atgaataacg tgctgccgtg gctgcacagc aaactggttc cggcaggcac
    12001 cgggcacgcc accgtaagct ggatcgtgcc ggagtaattg cagccattgc tggcacctat
    12061 tacgtctcgc gctacaatcg cggtaatcaa cgataaggac actctgtcat gactaaacac
    12121 tatgattaca tcgccatcgg cggcggcagc ggcggtatcg cctccatcaa ccgcgcggct
    12181 atgtacggcc agaaatgcgc actgattgaa gccaaagagc tgggcggcac ctgcgtgaac
    12241 gtcggctgcg tgccgaaaaa agtgatgtgg cacgcggcgc aaatccgtga agcgatccat
    12301 atgtacggcc ctgattatgg tttcgatacc accatcaaca agttcaactg ggaaacgttg
    12361 atcgccagcc gtaccgccta tatcgaccgt attcatactt cctatgaaaa cgtgctcggt
    12421 aaaaataacg ttgatgtaat caaaggcttt gcccgcttcg ttgatgccaa aacgctggag
    12481 gtaaacggcg aaaccatcac ggccgatcat attctgatcg ccacaggcgg tcgtccgagc
    12541 cacccggata ttccgggcgt ggaatacggt attgattctg atggcttctt cgcccttcct
    12601 gctttgccag agcgcgtggc ggttgttggc gcgggttaca tcgccgttga gctggcgggc
    12661 gtgattaacg gcctcggcgc gaaaacgcat ctgtttgtgc gtaaacatgc gccgctgcgc
    12721 agcttcgacc cgatgatttc cgaaacgctg gtcgaagtga tgaacgccga aggcccgcag
    12781 ctgcacacca acgccatccc gaaagcggta gtgaaaaatg ccgatggtag cctgacgctg
    12841 gagctggaag atggtcgcag tgaaacggtg gattgcctga tttgggcgat tggtcgcgag
    12901 cctgccaatg acaacatcaa cctagaagcc gctggcgtta aaactaacga aaaaggctat
    12961 atcgtcgtcg ataaatatca aaacaccaat attgaaggta tttacgcggt gggcgataac
    13021 acgggtgcag tggaactgac accggtagca gttgcagcgg gtcgccgtct ctctgaacgc
    13081 ctgtttaata acaagccgga tgagcatctg gattacagca acattccgac cgtggtcttc
    13141 agccatccgc cgattggtac tgttggttta acggaaccgc aggcgcgcga gcagtacggc
    13201 gacgatcagg tgaaagtgta taaatcctct ttcaccgcga tgtataccgc cgtcaccact
    13261 caccgccagc cgtgccgcat gaagctggtg tgcgttgggc cagaagagaa gattgtcggt
    13321 attcacggca ttggctttgg tatggacgaa atgttgcagg gcttcgcggt ggcgctgaag
    13381 atgggcgcaa ccaaaaaaga cttcgacaat accgtcgcca ttcacccaac ggcggcagaa
    13441 gagttcgtga ccatgcgcta aatgttaaag ggctaagagt agtgtgctct tagcccttaa
    13501 ttacgtttcc gctatcagtt cagaagctga agcagaaagc ggatcagttc cagcagcgca
    13561 attaacgctc caagaacgat gattgcttta tcaatcaccc gttttctcca tgcgatggag
    13621 tgagaatgca tccgcttact catccactgc ctgtcacggc gcatgtctca ttgttagatg
    13681 acgactatct cactccggcc agagcatcag ttaacggcac cacccgtact tctgaccagg
    13741 actttgaaag cgtttatgcg cattaccaga gtgaaaatgc ctcagagcta actggataat
    13801 catacagtac attcaggtta taaaaccagc atgtccttgc aatagtttca gtatggtatt
    13861 agcattgatg cgttagatga tgactatctc actccagcca gagccaccaa ctcagggctg
    13921 aagagtaaaa aaaccgacgc aaagtcggtt tttttacatc cggattcgga caaggcttaa
    13981 tatgacgatg acccagtgaa agtatataaa tcgtcattgc gatatatacc gaagtgcccc
    14041 ctccgccagc tgaagaaatc gctaattctt gcaatgttag ccactggcta atagtattga
    14101 accgttagat gtgggcattc tcactccagc cagagccacc aactcaggct gaagagtaaa
    14161 aaaccgacgc aaagtcggtt tttttacgtc ctgattcaaa ccgcctttca aatgaatagc
    14221 caactcaaaa ttcacaccta ttaccttcct ctgcacttac acattcgtta agtcatatat
    14281 gtttttgact tatccgcttc gaagagagac actacctgcc acaatcagga gcgcaatatg
    14341 tcatttctgt tacccatcca attgttcaaa attcttgctg atgaaacccg tctgggcatc
    14401 gttttactgc tcagcgagct gggagagtta tgcgtctgcg atctttgtac tgccctcgat
    14461 cagtcgcagc ccaagatctc ccgccacctg gcattgcttc gtgaaagcgg gctattgctg
    14521 gaccgcaagc aaggtaagtg ggttcattac cgcttatcac cgcatattcc agcatgggcg
    14581 gcgaaaatta ttgatgaggc ctggcgatgt gaacaggaaa aggttcaggc gattgtccgc
    14641 aacctggctc gacaaaactg ttccggggac agtaagaaca tttgcagtta aaaatttagc
    14701 taaacacata tgaattttca gatgtgtttt atccgggagg cattatgtta ctggcaggcg
    14761 ctatctttgt cctgaccatc gtattggtta tctggcagcc gaaaggttta ggcatcggct
    14821 ggagtgcaac gctcggcgca gtactggcgt tagttacggg cgtggttcat ccgggtgata
    14881 ttccggtggt gtggaatatc gtctggaacg cgacggctgc gtttatcgcc gtcattatca
    14941 tcagcctgct gctggatgag tccggctttt ttgaatgggc ggcgctgcac gtctcacgct
    15001 ggggtaatgg tcgtggtcgc ttgctgttta cctggattgt cctgctcggt gctgccgttg
    15061 ccgccctgtt tgccaatgat ggcgcggcgc ttattttgac gccgattgtc atcgccatgc
    15121 tgctggcttt aggattcagt aaaggcacga cgctggcgtt cgtgatggcg gccggattca
    15181 ttgccgatac cgccagcctg ccgcttattg tctccaacct ggtgaatatc gtttccgctg
    15241 atttctttgg cctcggcttt cgcgaatacg cctcggtgat ggtgccggtg gatatcgccg
    15301 cgattgttgc cacgctggtg atgctacatc tctattttcg caaagatatt ccgcagaact
    15361 acgatatggc gctgctgaaa tctcccgcag aagcgatcaa agatcctgct acgttcaaaa
    15421 ctggctgggt tgttttactg cttctgctgg tgggattttt cgtcctggaa ccgctcggca
    15481 ttccggtgag cgccattgca gctgtgggcg cgctgatatt atttgtcgtc gctaaacgcg
    15541 gtcatgcgat taatacgggt aaagtcctgc gcggtgcccc ctggcagatt gtcatcttct
    15601 cgctcggcat gtatctggtg gtttatggcc tgcgcaatgc cggattaacg gaatatcttt
    15661 ctggcgtact caacgtgctg gcggataacg gcctgtgggc cgcgacgctc ggcaccggat
    15721 tcctcaccgc cttcctctct tctattatga acaatatgcc gacggtactg gttggcgcgt
    15781 tgtccattga tggcagcacg gcatctggcg ttatcaaaga agcgatggtt tatgccaatg
    15841 tgattggctg cgatttggga ccgaaaatta ccccaattgg tagcctggct acgctactct
    15901 ggctgcacgt actttcgcag aagaatatga ctatcagctg gggatattac ttccgtacag
    15961 ggattatcat gaccctgcct gtgctgtttg tgacgctggc tgcgctggcg ctacgtctct
    16021 ctttcacttt gtaatgagat actgatatga gcaacattac catttatcac aacccggcct
    16081 gcggcacgtc gcgtaatacg ctggagatga tccgcaacag cggcacagaa ccgaccatta
    16141 tccattatct ggaaactccg ccaacgcgcg atgaactggt caaactcatt gccgatatgg
    16201 ggatttccgt acgcgcgctg ctgcgtaaaa acgtcgaacc gtatgaggag ctgggccttg
    16261 cggaagataa atttactgac gatcggttaa tcgactttat gcttcagcac ccgattctga
    16321 ttaatcgccc gattgtggtg acgccgctgg gaactcgcct gtgccgccct tcagaagtgg
    16381 tgctggaaat tctgccagat gcgcaaaaag gcgcattctc caaggaagat ggcgagaaag
    16441 tggttgatga agcgggtaag cgcctgaaat aaagcggcga tatccactgc cgatagttgg
    16501 taaagtgcgc tttgtttatg tcggatgcga cgctaacgcg tcttatccga cctacaaaac
    16561 cgtgcaaatt caatatatgg catgaaatat gtagagcgta gcgcatcagg caattttgct
    16621 atttacgcag cactcctcgc gtaagaattc aattatcgct tccagcattg cgtattgcga
    16681 aacacacatc agcgtccgac tctcacggtt ttgtttgacc agtcctaccg ataccagtgc
    16741 tgatatatgg tggctgagtg tggagccagg tatcccaagc tgcttttgca actcaccgac
    16801 cggcaaccct tgttccccgg ctttgacaag atgtttaaat atgaacaaac gggtcgggtg
    16861 acctagctct ttcagcgctt tagcgacttc ttccaactgc atatgacacc cctctgtctg
    16921 gttgatattt cgataatacc agaaatatct tgatctgaat ttcatttcga ttattctgga
    16981 aatatagatt tatccaatga ggttacgtat gagtagttgg cttgcgatgc tgcaagatgc
    17041 cgcagagatg tttgtgtttc tcgccgtcga gctttcgttg ctgtttatag tgattagtgc
    17101 cggtgtcagc ctgataagac aaaaggtgcc agaccataaa atccagcaga tgatgggggc
    17161 cagaaaaggg agaggctatc tcctggctgc tctgttggga gccgttaccc cgttctgtag
    17221 ttgctcgaca atacccatgt tacgtggatt gttgtcagcg aaagccgggt
//
LOCUS       DAZPJR010000081        16875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPJR010000081 DAZPJR010000000
VERSION     DAZPJR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.082"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHVB7_002500"
     CDS             92..1132
                     /locus_tag="ACHVB7_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR1914460.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHVB7_002501"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHVB7_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR1914461.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHVB7_002502"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHVB7_002502"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR1914462.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHVB7_002503"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHVB7_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR1914463.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHVB7_002504"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHVB7_002504"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR1914464.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHVB7_002505"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHVB7_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR1914465.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="ACHVB7_002506"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="ACHVB7_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /gene="araE"
                     /locus_tag="ACHVB7_002507"
     CDS             complement(9217..10635)
                     /gene="araE"
                     /locus_tag="ACHVB7_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR1914466.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="ACHVB7_002508"
     CDS             complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="ACHVB7_002508"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR1914467.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="ACHVB7_002509"
     CDS             complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="ACHVB7_002509"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR1914468.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12899..14080)
                     /locus_tag="ACHVB7_002510"
     CDS             complement(12899..14080)
                     /locus_tag="ACHVB7_002510"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR1914469.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14335..15564
                     /locus_tag="ACHVB7_002511"
     CDS             14335..15564
                     /locus_tag="ACHVB7_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1914470.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16024..16656
                     /locus_tag="ACHVB7_002512"
     CDS             16024..16656
                     /locus_tag="ACHVB7_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1914471.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         4456 a         4426 c         3898 g         4095 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggatatt ccaattttca aattaagttg aattattgag attattatta
    10801 accacctaat tttacagcag ataaaattca taaagttcat taattgataa ttgatatgga
    10861 ttatttcata accatgatat gaattatgat gacctacagg tataaaaaac cctgccatga
    10921 ggcagggtca taaaagtaag aggaatgaat taacgagcca gccaaccgcc atctacggca
    10981 atggtataac cattcacata atctgaagcg ctggaggcaa ggaacactat cggccccatc
    11041 aggtcactcg gcagtcccca acgaccagct ggaatgcggt cgagaatttc cgcgctacgt
    11101 tgttcatctg cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg agctatcgca
    11161 ttaacattaa tgttgtgttt agcccattcg ttcgccatca atcgcgtcac gcccatcacg
    11221 ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac
    11281 gtgatattga taatcttgcc gccattgcgt tgcgcgataa agtgtttcgc cgctgcctga
    11341 gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg
    11401 aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga
    11461 ccaaactcag ctaccgcgcg atccagcagt gctggaatgc catcaatctt tcgcagatcg
    11521 gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt
    11581 tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc
    11641 tggcccagtc cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca
    11701 cttaaaatca taacaatacc ttattcgttt atgccgacaa ctagcgcaaa tctttaacgg
    11761 ccactatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat catcaaagac
    11821 ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg
    11881 gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc
    11941 ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata
    12001 aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt tacctggagc
    12061 cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga caaaatattt
    12121 gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc
    12181 cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt
    12241 tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata
    12301 cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg taccggcacc
    12361 gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt
    12421 accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat
    12481 tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa
    12541 aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt ggatgctctg
    12601 tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga
    12661 aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta
    12721 aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg
    12781 aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc
    12841 atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct
    12901 actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca
    12961 gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac
    13021 cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct
    13081 catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct
    13141 cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat
    13201 acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc
    13261 gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag
    13321 ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg
    13381 aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat
    13441 caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc
    13501 gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca
    13561 actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat
    13621 cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa
    13681 gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc
    13741 caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg
    13801 ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta ttaggaagac
    13861 cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac
    13921 caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca
    13981 cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga
    14041 taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa
    14101 cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt
    14161 ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg
    14221 caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata cttaacctgg
    14281 tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca gataatgtct
    14341 aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac agccgttggt
    14401 gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt
    14461 actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc
    14521 ctctcatcaa aaacatcagc atgtgaaggg ataacgggcg cggtaacaca ctactatggc
    14581 aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg
    14641 atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt
    14701 gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt
    14761 ctgatgggac gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc
    14821 tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacgacc
    14881 caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt
    14941 ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat
    15001 ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc
    15061 ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc gtatattgaa
    15121 gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca
    15181 gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg
    15241 ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac actacagcag
    15301 gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt ggtatcgctg
    15361 attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta tgcgatcagc
    15421 ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta tctgatcccg
    15481 gcgcttaaac cctggcgctc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc
    15541 gtatcggtaa tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt
    15601 gaaccattcc atttttcata atattcattt gttattattc cgccctatca cttctctcgt
    15661 catatcctag aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga
    15721 ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg
    15781 cgatatagat attctcatac catgagatag ttatctggac cacgttcgac catgcaagat
    15841 taaatcagtg aaacataatc atatttaata acgagaaatg catttttaaa tgtcttaatc
    15901 agaattatag caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaagat
    15961 gtattgacat catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt
    16021 aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc
    16081 caaataatgc cagaagagtc accgcgtttg cagtattgcg caaactcgtg tttttgtatg
    16141 tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg aaggtatctt aacaaaaaac
    16201 cacaatgcga gattaagcgg atatattttt gtggattttt cagtaagttt tttacgtcta
    16261 ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat agttttagtc
    16321 agcgaccgca atatgcaatc gttagctaac tactggcgaa aacacaacac cgcaatatct
    16381 gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag acaactattt
    16441 attggtcgtt atttatcatt taccagaggt aacacattaa ctcagatgga attcaccata
    16501 atggggtata tggtttctgg ttataatcca tatcagattg ctgaagtgct ggatatggat
    16561 atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg taaaataaac
    16621 gaattattta ttcgttcaca ttcggtccaa cattgatact caacgaccag ccagaatcat
    16681 actctgttca tacgggcatc tattgaaatg cccgtatttt ataaataagc agtaacccca
    16741 agagtaaaca acatagtatt taaaaccatc acacgaaaaa ttcagaagca ttacgaaata
    16801 tgaatttaca aacaatagtg gcataaatgt taaccatgtt aatttacgta aagttttacg
    16861 ttgcaacatt aaagc
//
LOCUS       DAZPJR010000082        16593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPJR010000082 DAZPJR010000000
VERSION     DAZPJR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.083"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..586)
                     /gene="araH"
                     /locus_tag="ACHVB7_002513"
     CDS             complement(<1..586)
                     /gene="araH"
                     /locus_tag="ACHVB7_002513"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR1914472.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTT"
     gene            complement(601..2115)
                     /gene="araG"
                     /locus_tag="ACHVB7_002514"
     CDS             complement(601..2115)
                     /gene="araG"
                     /locus_tag="ACHVB7_002514"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR1914473.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2185..3174)
                     /gene="araF"
                     /locus_tag="ACHVB7_002515"
     CDS             complement(2185..3174)
                     /gene="araF"
                     /locus_tag="ACHVB7_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR1914474.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            3971..4474
                     /locus_tag="ACHVB7_002516"
     CDS             3971..4474
                     /locus_tag="ACHVB7_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR1914475.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4553..4804)
                     /gene="yecJ"
                     /locus_tag="ACHVB7_002517"
     CDS             complement(4553..4804)
                     /gene="yecJ"
                     /locus_tag="ACHVB7_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR1914476.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(4919..5005)
                     /gene="azuC"
                     /locus_tag="ACHVB7_002518"
     CDS             complement(4919..5005)
                     /gene="azuC"
                     /locus_tag="ACHVB7_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR1914477.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5268..5591
                     /locus_tag="ACHVB7_002519"
     CDS             5268..5591
                     /locus_tag="ACHVB7_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR1914478.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5762..6259
                     /gene="ftnA"
                     /locus_tag="ACHVB7_002520"
     CDS             5762..6259
                     /gene="ftnA"
                     /locus_tag="ACHVB7_002520"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR1914479.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6297..6536)
                     /locus_tag="ACHVB7_002521"
     CDS             complement(6297..6536)
                     /locus_tag="ACHVB7_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR1914480.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            6727..7938
                     /gene="tyrP"
                     /locus_tag="ACHVB7_002522"
     CDS             6727..7938
                     /gene="tyrP"
                     /locus_tag="ACHVB7_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR1914481.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(8000..8665)
                     /gene="yecA"
                     /locus_tag="ACHVB7_002523"
     CDS             complement(8000..8665)
                     /gene="yecA"
                     /locus_tag="ACHVB7_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR1914482.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(8861..8947)
                     /locus_tag="ACHVB7_002524"
     tRNA            complement(8861..8947)
                     /locus_tag="ACHVB7_002524"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8911..8913),aa:Leu,seq:taa)
     gene            complement(8960..9033)
                     /locus_tag="ACHVB7_002525"
     tRNA            complement(8960..9033)
                     /locus_tag="ACHVB7_002525"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8999..9001),aa:Cys,seq:gca)
     gene            complement(9088..9163)
                     /locus_tag="ACHVB7_002526"
     tRNA            complement(9088..9163)
                     /locus_tag="ACHVB7_002526"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9128..9130),aa:Gly,seq:gcc)
     gene            complement(9315..9863)
                     /gene="pgsA"
                     /locus_tag="ACHVB7_002527"
     CDS             complement(9315..9863)
                     /gene="pgsA"
                     /locus_tag="ACHVB7_002527"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR1914483.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(9920..11752)
                     /gene="uvrC"
                     /locus_tag="ACHVB7_002528"
     CDS             complement(9920..11752)
                     /gene="uvrC"
                     /locus_tag="ACHVB7_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR1914484.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11749..12405)
                     /gene="uvrY"
                     /locus_tag="ACHVB7_002529"
     CDS             complement(11749..12405)
                     /gene="uvrY"
                     /locus_tag="ACHVB7_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR1914485.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            12701..12877
                     /gene="yecU"
                     /locus_tag="ACHVB7_002530"
     CDS             12701..12877
                     /gene="yecU"
                     /locus_tag="ACHVB7_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR1914486.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            12864..13088
                     /gene="yecF"
                     /locus_tag="ACHVB7_002531"
     CDS             12864..13088
                     /gene="yecF"
                     /locus_tag="ACHVB7_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR1914487.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(13155..13877)
                     /gene="sdiA"
                     /locus_tag="ACHVB7_002532"
     CDS             complement(13155..13877)
                     /gene="sdiA"
                     /locus_tag="ACHVB7_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR1914488.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(14107..14859)
                     /gene="tcyN"
                     /locus_tag="ACHVB7_002533"
     CDS             complement(14107..14859)
                     /gene="tcyN"
                     /locus_tag="ACHVB7_002533"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR1914489.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(14856..15524)
                     /gene="tcyL"
                     /locus_tag="ACHVB7_002534"
     CDS             complement(14856..15524)
                     /gene="tcyL"
                     /locus_tag="ACHVB7_002534"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR1914490.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(15539..16525)
                     /gene="dcyD"
                     /locus_tag="ACHVB7_002535"
     CDS             complement(15539..16525)
                     /gene="dcyD"
                     /locus_tag="ACHVB7_002535"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR1914491.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         4189 a         4277 c         3843 g         4284 t
ORIGIN      
        1 aggtggtttt attcagcaac aaaccaaaga taatcagaca cgcgacggtg agccagattg
       61 gcgcaggcag accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac
      121 cgaccgcttt accgtctgaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca
      181 gtgttgtgat cagagcattt attttcagtt tggcgataac aaagccattg accaggccac
      241 agagaatgcc aagcagcaac cccgctgcca cgccaatcca caggctttca gtcaggttga
      301 taaccaccgc cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt
      361 caccggaagc aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca
      421 ggcccaaccc tttcatatta atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga
      481 taaagagcac cgcaaacacc accagcatgc cgtactgatc ccagatacgc ccgaagctga
      541 atgacgactt aggtgcgcca gaacccgatg tagaaacaga agacatcata ctctccttac
      601 tcaggcaact gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc
      661 ctgctcgtgt aacaattcac cggcgatttc accttcccgc atcaccacaa tccggtcggc
      721 aacgccgagg acttcaggta agtcgctgga ggcaaacagc accgccacac cctgcgccgc
      781 cagcgcataa attacgttat atatttcgtg cttagcacca acatcaatgc tgcgcgtagg
      841 ttcatccagc aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg
      901 ctgatttccg cctgagagat tcatgatcag ttgctcagcg cccggcgttt tgatgttgag
      961 cgaacgaatg tgctgatcgg cattgttttc ttcccaaccg ttgttgatta cacaaccgcc
     1021 gagcacatgt ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt gcacgggaat
     1081 aatgccttcc gctttgcgat cttccgggca gagcatcatg cctgcggcaa tggcgtggct
     1141 cggtttacga atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt
     1201 cccgccaaac aagcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc
     1261 aacaatttca ccactgcgaa ccgccagact tattggcgta cgcacgcctg gtgctttcac
     1321 agcatcaaga cgcaggcgct cctcgccata actacgaggt tgccagccgt agatatcgcc
     1381 aatgtcgcgc ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt
     1441 aaaggttttg acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat
     1501 ttcttccata cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat
     1561 aacgcggaaa agattgtcga tttcgcgggc agaaagggag ctggttggct catcaaaggc
     1621 gataatttta gcgttacgcg ccagcgcctt ggcgatttca accatctgcc actgaccaat
     1681 ggagagatat ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa
     1741 acccgcctca taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc
     1801 gagatagatg ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat
     1861 cgccacaccc gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat
     1921 cactacagaa cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt
     1981 tcctgcgcca ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat
     2041 atccgtcagc gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg
     2101 ggtagactgt tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat
     2161 gcagagggga atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc
     2221 tttaaagttg tcacgcgtga tcagcaccac gtcggtaact tcggtaaatt ttggcggttc
     2281 aacgtctttt gctacccagt tgtaaagcat ttcgctggat ttatagccat gtacgtccgg
     2341 gcttggcagc agggaaccgt agaagccggt tgcctgtgct ttagacagtt cgctcaacgc
     2401 atccacaccg ttaatgccaa tgccgatgat atcggcagct ttaaagccct gaccttccgt
     2461 cgcgcgtacg ccgcccagca cggtgctgtc gttcataccg acgatcagcc aatgtttaac
     2521 ttccggatgt tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga
     2581 tttggtaggt acctgataaa tttgtttttc cgggaatccg gccgctttca gcgcatccat
     2641 agaacccgtt gtacggcgac gtgccgtatc cagttcgttg gcggtaatcg ccatcaccgc
     2701 gctttctttg acatcccagc cacgtttctg catctcttta tacagttcct gaccctgacg
     2761 ttcgccaatt ttagtcgccg ccatcatcac cagcggaacg gtatccattg gcttaccttt
     2821 ggcgttaaca aactggtcat ccacggcaat gactttcata tcgtagccac gcgctttcgc
     2881 gacgatggca gagccgagtt tggggtccgg agtacaaata acgaaacctt ttgcgccact
     2941 ggcagccagg ctgtcgatcg cgttcaatgt tttttcgcca tccggcacgg caatcttaat
     3001 aacctcaaac cctaaatcct tcccggcttt atcggcaaac ttccattcgg tctggaacca
     3061 cggctcttcc ggttgcttca ccagaaaacc gagcttcagg ttctccgcca tagcggattg
     3121 tgacataacg gctgccagac caatggctgc cagggcttta gtaaatttgt gcatggttct
     3181 ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat gactttcgtt aaattaatcg
     3241 gaaaacaaag cattaccttt tatctaaaag ataagtgacc gtgttgacat agttttagcg
     3301 agaaattaat tctccatagg agagcaatat cacatcgcag aattacagtg agaacgtgca
     3361 taaatttagc gggaaaagac ataagggaaa gccaatttgt cagacaaatt gtcgaatgca
     3421 cagcagatta atccataaga ttagcctgga aatccttgtt gtctttggta cccatgcggg
     3481 atgtcttctt tttaaccagt caataggccg cattacctgg cgttgagttt ttgaaatggt
     3541 gtaataaccg caactcaaag atgtggaaaa tgcacgtcat tcatttcgcc attaattatc
     3601 actgtgctca ttaattaaca gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa
     3661 cgttttataa aatcatcact tcatcatgaa ttcaaattca ttgattaata tcaacaagat
     3721 acaaaaagca ctatcattaa aattcattgc agttacattg atttcatcaa tgaaatgtaa
     3781 aaatatataa acttgatgat ttaagcattt tcttataccc gttcagacgt tattcttatt
     3841 tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt
     3901 ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc tggatataag
     3961 gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga
     4021 gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa
     4081 cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat
     4141 gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttcccgg
     4201 tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg
     4261 ttctagtacg ttggcacagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt
     4321 caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggtctgttgc tgcaaaccat
     4381 tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca
     4441 tgttctgaat gtcgtgtcac accagctgca ttgatcatca tcggcgctaa tgcattgcgc
     4501 cgatgaaggt tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg
     4561 attcaccctg tggggtaaac ttgagttcaa taagcgcgat ggctttttgg attgcccgca
     4621 tggtgaccgg gtctgcggcg gcgggatggt tagtaaagtc gatattcttc agctgactgg
     4681 acattttttc acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga
     4741 taaccaactg gcaagcaacc acatcagaaa ccagttcctg atcggcattc agcggctggg
     4801 acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag
     4861 cgatagtcac atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt
     4921 atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac
     4981 ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat
     5041 tgtctttgcg cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc
     5101 acataaatac ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg
     5161 acacctacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc
     5221 gctgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga
     5281 ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccaa gcccatgtga
     5341 gcgaagttga cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc
     5401 atgcccatac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag
     5461 gctataccca cgcggtcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt
     5521 ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg
     5581 tctttctcta aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa
     5641 tgcgttttat tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat
     5701 aataaaataa caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat
     5761 catgctgaaa ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc
     5821 ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc
     5881 cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta
     5941 cctgactgat accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata
     6001 ttcctcactt gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa
     6061 aattaacgaa ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct
     6121 gcagtggtat gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa
     6181 attaagcctg gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac
     6241 cctcgacaca caaaactaat gctctccggc ggcaggttta ctgccgccgt ttcaaatcag
     6301 tgacggcaaa tcttactctg atcggtcgaa aaaccgtctt tcgaaggtat aaatttgcct
     6361 tttgctgcca gaaacgctac cagctctccc gctgtcatcc cttctgccga gcaggtgtga
     6421 aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt ccagactggc atgcgtatat
     6481 tgctcgcctg attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt
     6541 cctcctcaaa aaaagcctag cgtagcgatt gccgcttatg aagactttgc gccagcgcag
     6601 gactgaatgc tttttattgt acatttatat ttacactata tgtaacgtcg gtttgacgaa
     6661 gcagccgtta tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa
     6721 gaaagcgtga aaaacagaac cctgggaagt gtttttatcg tggcgggaac cacaattggc
     6781 gcaggcatgc tggcaatgcc gctggctgcg gccggtgttg gttttagcgt tacgttaatc
     6841 ttgttgattg ggctttgggc gttgatgtgc tacacggcgc tattactgct ggaggtgtac
     6901 cagcatgttc cggcagatac cggtctgggc acgctggcaa aacgctatct gggacgctac
     6961 ggtcaatggc tgacgggctt cagtatgatg ttcttaatgt atgctctgac tgcggcatac
     7021 atcagcggtg tcggtgaatt gttggcctcc agcatcagcg actggacagg tatttcaatg
     7081 tcggcaaccg ctggcgtgct gttgttcact tttgttgccg gtggcgtggt ttgtgttgga
     7141 acatcgctgg tcgatttatt taaccgtttt ctgttcagcg ccaaaattat ttttctggtg
     7201 gtgatgctgg tattgctgct accgcatatt cacaaagtga atcttttaac cctgccgttg
     7261 caacaggggc tggctctgtc agcaatcccg gtgattttta cctcatttgg ttttcacggt
     7321 agcgtgccga gtattgtcag ctatatggat ggcaacgttc gtaagctacg ctgggtgttt
     7381 ataaccggta gtgcgatccc cctggtggca tatattttct ggcaggtggc aacgcttggc
     7441 agcattgatt caacaacctt tatgggattg ctggctaatc atgctggatt aaacgggctg
     7501 ttacaggcgt tacgcgaaat ggtggcctct ccgcatgttg agctggcagt gcatttattt
     7561 gctgatttag ccctcgccac gtcatttctc ggcgttgcgt taggcttatt tgattatctg
     7621 gctgatttat ttcagcgttc aaataccgtt ggtggacggt tgcaaactgg tgcaattacc
     7681 tttctgccgc cgttggcgtt tgcactgttt tatccacgag gatttgtgat ggcgctgggt
     7741 tacgccggtg tggcgctggc ggtactggca ttgattatcc cttcgctgtt gacctggcaa
     7801 agcagaaagc acaatcctca ggcgggttac cgggtcaaag gtggtcgtcc ggcgctggtg
     7861 gtggtgtttc tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc ggcagggttg
     7921 ttaccagaag tggggtgatc agatagcctc aaattcctta ttgggtgcca gaattaacgc
     7981 tgacacccaa tttggcctct taatgcaggc agcactgctt aaatttctta ccactaccgc
     8041 acgggcaagg atcgttacgc cccggtttct cttctgcttt gatcggttgc tgaacagctt
     8101 tttcctgcgg atgcgccatc cagtacgcat gtagatcaag cgccgccagt cgaatggcat
     8161 ctacgctctc ttcaaacgct tctggcgaca tcttttctac ccgctcgaag ttttcctcag
     8221 taccgtgcag cgcaatcgcc tccagcgctg gttttaacga atcgggcaac gttgaccagt
     8281 cagaaagtgc cacgccccgc atatagccaa agcaccactc ctcaacaatc gtcagctcgc
     8341 tgccatcaac ttctcgcaag ccgaataacg gctcaaactg ctccgggaat tcgttcagac
     8401 gctctgcggt atcggccata tgttgaaaag ccaggttcat aaagcgcgtc atctctttct
     8461 ctgacgccca gcgcggcaca tagtcagccc caccccacac ggcaaccagc cactgttccg
     8521 gttcaatctc ttgcggagaa ctcaacaccg ccgtcaataa accgtccagc tccgccacat
     8581 caaggatggc gtggttagtg ttgtatttgg tcagaatatc gtccagccat tccaactcac
     8641 tttcgtttaa cggtcccgtt ttcatacgct tttccttgtg gatctcaact cgccagcacc
     8701 tatcttacat gccggtccgt atcagagata ctttttgagt ggctttgccg gtgattaaaa
     8761 attaaggagg gtgtaacgac aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac
     8821 gacactgctt attgctttga ttttattctt atctttccca tggtacccgg agcgggactt
     8881 gaacccgcac agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc gattccacca
     8941 tccgggctcg ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta gacggatttg
     9001 caatccgcta cataaccgct ttgttaacgc gccaaattct tcaggccttt cagccagaca
     9061 tccgcttgac gccgatgtct tttaaactgg agcgggaaac gagactcgaa ctcgcgaccc
     9121 cgaccttggc aaggtcgtgc tctaccaact gagctattcc cgcattcatc aagcaatcag
     9181 ttaatcactt gattttatta tcgtctggca atcagtgccg ccgttcgatg cgttgcattc
     9241 tacttacctg gcgcgatgag tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa
     9301 ttacgccgaa acgatcactg atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac
     9361 attgaccaca gagtcagtac cgcagccaca aagaaaagtg caataccggc gtactcaacc
     9421 caaatgttcg gacgccacag cagccatgcc aacgccacca tctgggcagt ggttttcact
     9481 ttcccaatcc aggagacagc cacgctactg cgtttaccca actccgccat ccattcgcgt
     9541 agcgcagaaa taataatttc acgggcgatc atcgttgctg ccggtaaggt cacccaccag
     9601 ctgtggtaat gctcggttac cagcaccatg gcgatagcca cgagaacttt atctgctaca
     9661 gggtcaagga aagcaccaaa ccgggtactc tggttccagc ggcgtgccag aaaaccatcg
     9721 aaccagtcag tcaccgccgc gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg
     9781 acaggcagat aaaagaccaa tacaaagaat gggataagga tgacacggaa cagtgtaagc
     9841 aacgtaggga tattaaattg cataatgacg ggtaactatc tgttgtcagt aagattaccc
     9901 ctatgttgct acagagacat caatgtttca acgaccagaa gatcttttct gccagacctt
     9961 gcgaaatacc cggcactttt gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac
    10021 cgcccatata tttcaacaac atttgccgac gttttggccc gacgccttca atggtttcca
    10081 gggaactggt atttttgacc ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcat
    10141 gtgattcatc gcgaatatgc tggataacat gcagcgcggg agaatctggc ggcaaactga
    10201 atccctcacc ttccggctca aagaacagcg tttccagccc agccttacga tctgctcctt
    10261 tggcaacgcc aagtagcagc ggatgatttt tatcccatga gacatccagt tcggcgaaga
    10321 catttttcgc ctgcgcaagc tggcctttgc cgccgtcgat aaggatcaca tccgggatct
    10381 tactgtcgtc aatggcttta ccataacgcc gacgcagcac ctgattcatc gccgcataat
    10441 catcgcccgg cgtgatgcct gtaatgttat agcgccgata ctccgcacgc agcgggccgt
    10501 tagcatcaaa caccacacag gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa
    10561 agcactccat ccgcttcact tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct
    10621 ggtgaacggt agattgctgc gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg
    10681 cgagtttcag ataacgcgcc ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc
    10741 ccgccagttc tgaaagggaa tcggcgagca gcgttttatc gctaagatta aaatcgagca
    10801 ggatctcacc cggtaaggtg cgcatctggc tgccttgtaa atagaactgg cctacgaagg
    10861 tttctaccac ctcgctcagt tccgtaccgc caggcacttt cgggaaatag ctgcggctgc
    10921 cgagcacttt gccctgacga atgaacaata cgtggacaca agccatgccc gcatcgaacg
    10981 ccacaccaat aacgtcgagg tcgtcgccgg tattggaaac gaattgtttt tcggtgacgc
    11041 gtcgcaccgc ctgaatttgg tcgcgaatac gtgcagcttc ttcaaactcc agattctggc
    11101 tggcagtttc catacgacta atgagttgcg taagcacctg atcatctttg ccagacaaaa
    11161 acaggcgcac atactcaacc tgctgagcgt attcttcttc actcaccagt ccttcaacgc
    11221 acggtcccag acagcgccct atctgatatt gcagacacgg acgcgagcga ttgcgataaa
    11281 cactattttc gcactggcga atggggaaaa tcttttgcag tagcgccagt gtttcacgta
    11341 cggcatagcc attcgggaac gggccgaaat attcaccttt ggcatgcttc gcaccacgat
    11401 gcatcgccag acgcgggtgg gtatcgccac tcaggaagat aaaaggataa gatttatcat
    11461 cgcgtagcaa aacgttgtaa cgcggctgat agagtttgat gtagttgtgt tccagcaaca
    11521 gcgcttcggt ttctgtatgc gtaaccgtta catcaatttg ctggatctgg gcgaccagcg
    11581 cttcggtttt gcgcgaagcg aggttgctgc ggaaatagct ggaaagccgt tttttcaggt
    11641 ctttcgcttt gccgacatag ataaccgtac caccagcatc gtacatgcga taaacgcctg
    11701 gctggcaggt tacggttttt aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga
    11761 taatgtctcc gcattacaca gaccatggcg aattgccagg tgagtcagct caacatcgcc
    11821 atgaatgttt agtttactga acatacgata gcggtagctg ttcaccgttt tcggactgag
    11881 attgagctgt tctgagatct cattgacctt ctggcccttg gtgatcatca gcataatctg
    11941 caattcacgt tcagacaaac tggcaaatgg gctttctgtt ttttctggtt cgatctggct
    12001 taacgccatt tgttgagcga tatcagaagc aatgtaacgc tgccctgaat agacagaacg
    12061 aatcgcactc acgacttcct gcggagccgc gcctttgctg aggtagcccg cagcaccggc
    12121 ctgcatgact ttcgctggta aagggttttc tgtatggacg gtaagcatga tgattttgac
    12181 atcagctgtg gaacgcgcga ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact
    12241 catgtccatt agcaccacgt caacggcatt tgtccggcac cacttaacgg cgtcttcacc
    12301 gcacgatgcc tcaccgacga cttttatacc ctttatatct tccagaatgc gtcgtatccc
    12361 tgcgcgcacc agttcgtggt catcaacaag tagaacgttg atcaaaggaa tatctccaga
    12421 aatagggata acgctactga tagttagtca ttcgtatatt agcggttttt attgcaactt
    12481 tgaaacgtta aaaatggtgg gtattcgatt tttctctcgt ttttgtaaat tcgattgtcc
    12541 acagttatgg tcacgcccgt caccaacggc ttccgggatc gtttcacagt aagcccggcg
    12601 atgaaatgtg acaaaaattg actttattca gcaaaaatga aaatcagccc gctgaatatg
    12661 taacattaat taacccgaac aatacgggca aaaaacattc atgataaaaa tatttattgg
    12721 tcattatatt aacgcattta atagcattgt tgacgtcacg ctcaaaaaac aaccactgct
    12781 atttttagca aagcttatgg tatactccgc cgccttaaca tttttcaccg caaattttca
    12841 ttgcaacatg acgaggaaaa taaatgagta cgcctgattt ttctactgcc gagaataatc
    12901 aagaactggc aaatgaagtc tcctgcctga aagcgatgct gacgctgatg ctacaggcga
    12961 tgggacaagc tgacgcgggc cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg
    13021 aagacgaaac ccaggctgca gtattttcca aaacggttaa gcaaattaaa caagcctacc
    13081 gtcagtaata taaaaccggc tgagagcgtg cctttcagcc gatttttgca tctggcacgc
    13141 agaaagaaaa gcgatcaaat taagccagta gcggccgcgt aacaggccac ctgggtctta
    13201 tttggtgcat tgattttttt ctgcatgttt ttctgatgga aattgaccgt attctcagag
    13261 attgacaaaa tcatcgctat ctctgctgat gtcttccctt ccgccgtcca tttcagaatt
    13321 tctttttcgc gcttgctgaa attcatctct ggcgtcatca ctatttcatc atttaaacgc
    13381 atcagagcca tcagactttc gcgcaccagt aactgcattt ttaattgcag ttcatcacta
    13441 agaatgggta tttcgcgcgt gctgcaacgg gaaaaggaca aaaagcccag cgcccgattt
    13501 ggcagcatta aatactgagt gacaccgcgg cgtaaaccat gtgcgtgcgc ggcttcccat
    13561 aatggctgtg cttcgctgaa taagtcatca ttccacatta aatggccctg actaaagttt
    13621 tcagggttca gcacaggatc aattgcgaga aaattttttg cctgataata actaacccac
    13681 gactcagggt aattggtgta gaaagccact ttaggtcgcg tgaatggtac cgggtggcga
    13741 acacataacg aatagtaatc gtactccagc tgctgagcct gaagctctat ttcatggtag
    13801 acctcttctg cggcctccat cctctgaaaa cgcaacagca tcgtgcgacg ccagctgaaa
    13861 aaatccgtat cctgcatagt aaaccgcaac gcccctgaga gtgagtatca tttataatga
    13921 taattcaaac tatcacataa ataagattta tatataattt atattattca ggcaatgaat
    13981 tacttttgca agccatcgca ttctcttatg ttattaatga gttatgctga tttgttaagc
    14041 agttttatca cgcttgaaat ggcgtcccgc cccgacaggt gaatcgtcgg ggctgatttt
    14101 ttcttattat tgcagtagaa acttctcgag gaactggcgg gtgcgaggct gctggggatc
    14161 agcaaataac gcttttgctg ccccctgctc gactatccgc ccctggtcca taaaaatcgc
    14221 ccggtccgca acatcccggg caaagctcat ttcgtgcgtc acaatcacca tcgtgcgctt
    14281 ttcctgcgcc agctggcgga ttgtgttcag gacttcgccc accagctctg gatccagcgc
    14341 tgacgttggc tcgtcaaaca aaatcacctc aggacgcatt gccagcgcac gcgcaatcgc
    14401 tacacgctgc tgttgaccgc cagacaaacg acgtggatag ctggtttctt taccggccag
    14461 cccaactttc gccagcagct cgcgagcgcg cgccgtggcc tcttctttcg gttcgccttt
    14521 gacgatcacc ggcccttcaa taatgttttc cagcaccgta cgatgcggaa acaaattaaa
    14581 gttctggaag acaaacccga cgtgctgacg caactggcgg atcagcgatt tttgctgact
    14641 taatgaacgt gcagtatcaa tagtgatatc gccaacggtg atcgttcccg cttccggttg
    14701 ttccagcaga tttatgctgc gtagcaacgt ggttttgccg gaaccactcg gaccgataat
    14761 tgccaccact tcgccaggct ttacctcgag gtcgataccg tgcagcaccg tctgaccgtg
    14821 aaattttttc accaggttct taacttcaat ggcactcatt ttggttctct ctcctggcga
    14881 ttaagttgat tctcaaaatg gttctgcaac gtcgataata ctgtcgccat gatccagtag
    14941 atgagcgaag ccgccaggta catggtgaac acctccagcg tgcgcgaggt aatcaactgc
    15001 gcctgacgga acagctccgg cacctggatt gtcgcggcca gcgacgtatc ctttaccagg
    15061 ctgataaagc tgttcgacag cggcggcaac gccacccgcg ccgcctgtgg caaaatcgcg
    15121 cgacgcatag tctgccatgg cgtcatacca atactggcag ctgcttccca ctgaccttta
    15181 tcaatagaag aaatggcggc gcgcagtgtt tcggcggcat aggcggcagt gttcagcgac
    15241 agaccaatca ttgctgacgg aatgggatct aattcgatac caaactgcgg caggccgtaa
    15301 tagatcataa acagttgtgc gatgagcggt gtaccacgaa aaatagagat ataaaaacgc
    15361 gccagccagc gcacgggcca gattggcgac aggcgcatca gcgcgagaat aaacccgagc
    15421 agtaagccaa aaaacatgcc gccgatactg agttgcagcg tatacccagc ccctttcagc
    15481 aggaacggca aggaatcaat aaccagttgt atactttctt gcatgagcgt tcccggagct
    15541 aaacgtgggg atgataggcg aacagcgcag gcgcaccgcc ggtatgaata aacagaatcg
    15601 gcccttcatc tttgaagcgt ttctgactga tacagccaat cagccccgcc atcgcttttc
    15661 cggtatacac aggatcaagc agaatgcctt ccagccgcgc cagcaatttc actgcttcca
    15721 tgccttcgtc gttcggcacg ccgtagccag gtgcaaaata gtcatcccag agtataattt
    15781 ccgctgatgc ggtcagctcc agttctttcg caatcgcctg ttgtaggtta accactttcg
    15841 gcaactgatc ggcaacggaa cgcgacacgg tcacgccaat cagttcgctt tcaggcatca
    15901 ggtgttccag cccaacagcc agtccggcgt gagttccggc actgcccgat gcgactacca
    15961 ccgacgaaat attaaccgcc ccttcacact gttgcgcgat ttccagcgca ctctccacat
    16021 aacctagcgc gccaagagca ttagaaccgc caaccggaat gacatacggg cgaaagcctt
    16081 gtgcttcgac tcgcgtcgcc agctcttcca gttgggcatt gggatcggtc agtgcttcgc
    16141 acatttcaat ctgggtattg aacagatcca gcaacaagcg attgccgttg gttaaatagt
    16201 tttctgcggt tgtgccaata ggattttcca gcagcgccac acagtgcaga ccgagtttcg
    16261 ccgcaactgc ggcagtctgc cgcacatggt tagactggat caccccggca gtaatcagcg
    16321 tatcggcacc ttcgcgcaga gcatctgccg cgagaaattc tagcttacgt aatttattgc
    16381 cgcccattgc catgggggtg acgtcatccc gtttgatgaa aatttcccgt cctagataat
    16441 cagaaaagcg cggcagatat tcgagcggcg ttggcgcgcc gataaactcc aaccgtggaa
    16501 aacgggttaa attatgcagt ggcataacag cctccgatgt atgttgttgt gattttctta
    16561 ttatgcacgc tgaaaacgcg taaataaaaa agg
//
LOCUS       DAZPJR010000083        16565 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPJR010000083 DAZPJR010000000
VERSION     DAZPJR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.084"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(133..285)
                     /locus_tag="ACHVB7_002536"
     CDS             complement(133..285)
                     /locus_tag="ACHVB7_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1914492.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(550..1284)
                     /gene="cysH"
                     /locus_tag="ACHVB7_002537"
     CDS             complement(550..1284)
                     /gene="cysH"
                     /locus_tag="ACHVB7_002537"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1914493.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1359..3071)
                     /gene="cysI"
                     /locus_tag="ACHVB7_002538"
     CDS             complement(1359..3071)
                     /gene="cysI"
                     /locus_tag="ACHVB7_002538"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR1914494.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3071..4870)
                     /gene="cysJ"
                     /locus_tag="ACHVB7_002539"
     CDS             complement(3071..4870)
                     /gene="cysJ"
                     /locus_tag="ACHVB7_002539"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR1914495.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5189..5551
                     /gene="queD"
                     /locus_tag="ACHVB7_002540"
     CDS             5189..5551
                     /gene="queD"
                     /locus_tag="ACHVB7_002540"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR1914496.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5629..6900
                     /locus_tag="ACHVB7_002541"
     CDS             5629..6900
                     /locus_tag="ACHVB7_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1914497.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6891..7151
                     /locus_tag="ACHVB7_002542"
     CDS             6891..7151
                     /locus_tag="ACHVB7_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1914498.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7168..7743
                     /locus_tag="ACHVB7_002543"
     CDS             7168..7743
                     /locus_tag="ACHVB7_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR1914499.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7891..8751)
                     /locus_tag="ACHVB7_002544"
     CDS             complement(7891..8751)
                     /locus_tag="ACHVB7_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1914500.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8748..9527)
                     /locus_tag="ACHVB7_002545"
     CDS             complement(8748..9527)
                     /locus_tag="ACHVB7_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR1914501.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9505..10914)
                     /locus_tag="ACHVB7_002546"
     CDS             complement(9505..10914)
                     /locus_tag="ACHVB7_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1914502.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10936..12390)
                     /locus_tag="ACHVB7_002547"
     CDS             complement(10936..12390)
                     /locus_tag="ACHVB7_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR1914503.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12460..13276)
                     /locus_tag="ACHVB7_002548"
                     /pseudo
     CDS             complement(12460..13276)
                     /locus_tag="ACHVB7_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13594..14871
                     /locus_tag="ACHVB7_002549"
     CDS             13594..14871
                     /locus_tag="ACHVB7_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR1914504.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            14898..16383
                     /locus_tag="ACHVB7_002550"
                     /pseudo
     CDS             14898..16383
                     /locus_tag="ACHVB7_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3940 a         4359 c         4166 g         4100 t
ORIGIN      
        1 taattttcat ccgtaatata tatgaataat aaacataggc ataatattca cataaataat
       61 ataaattaaa aaagggtgca ggagaccaaa ccatcacaat tcagtacgcg gggttttacc
      121 cccgcgtggt ggctacttct tcgattcgta agcgagaact gccttaaact ccataccacg
      181 ttctctgata ctcaactcac acaacgagtc gcctaccatc agcgtgaatc ccagtactgt
      241 aaaacacagc actatgattg ccacaagggc atattttgtc agcatgtctt gcctccctgc
      301 aaagaagaga ctaacattcg aattgtttgg gttcgaacgc tggcctcagg ttgatagaaa
      361 tatcgcctgg ggcttttgtc catctggaac ctcgcgaatg cttaacgcca gacagcctca
      421 agcacccgac gccattctat acctgataat tcttccagcg gcgttcacgt ttaaaatcag
      481 gaatactgct cgcaaaataa aaacgccccc atcatttctg acagaggcgt ttaatttatc
      541 cggcaatatt tacccttcgt gcaatccaca ttcccttttt aagccaaaga aacgtgtttc
      601 ttcttccgac atgccaggtt cccatttacg ggttgtatgg gtatcaccga ccgataaata
      661 tccttcatcc cataatgggt gatatttcag gccatgtttt tgcaggtact gataaatagt
      721 tcggttatcc cagtcgataa tcggcagcac tttaaatacg ccacgctgaa ttgccagcac
      781 cggtaaattg gcgcgactgc cggattgttc acggcgcagg ccagcaaacc aggtttgcgc
      841 gttaagttct ttcagagccc ggttcatcgg ttcgactttg ttgatgtcat tgtacttttc
      901 aatgccttca acgccctgct cccacagttt tccgtagcgt gcttcctgcc aggctgcgct
      961 ttcggtagca cggtacactt tcaggttgag cttgagtttg tccgttaact cgtcaataaa
     1021 gcggtaggtt tccgggaaca agtaacccgt atcggtgagg atcaccggaa tatccgggcg
     1081 aatttgattc accagatgca ggctcaccgc cgcctgaatg ccaaagctgg aagaaagtac
     1141 atattcaccg ggcagattat ccagcgccca ggctacgcgg ccttcagcgt ccagtttttc
     1201 cagttcggcg ttagtttccg ccagcgccag aatacgatct accttcggca gttcgttcag
     1261 ggcgtttaga tcgagtttgg acatagattc ctcactgttt gccttgcctg atgcgacgct
     1321 cacgcgttct tatcaggcct acaagaccgg gctgatggtt aatcccacaa atcgcgcgcc
     1381 ggatcgagca ccgggcgaat gatgcccgca cgcaccgtaa agtcgccgaa gccttcaccc
     1441 gcttcgcgct ctttcgccca gcgccctatc agttcatcaa gcgacgccag gatttccggc
     1501 tcggtgatgt tttctttata catccgtggg atacgtgtcc caatgcggtt gccgccaaga
     1561 tgcaggttgt agcgacccgg cgctttaccc accaggccca cttccgccag catcgcgcga
     1621 ccacaaccgt tcgggcagcc tgttacacgc atcacgatat gctcatcgct gacaccatgt
     1681 ttcgccatta aattatcgat gttgtcgata aaagacggca ggaaacgctc tgcttccgcc
     1741 atcgccagcg ggcaagtcgg gaatgacacg caggccatcg agttttcacg ctgcggcgtg
     1801 acggcattca ttaacccgct ctctttggcg atcttctcga tcttcgcttt ctcgctttcc
     1861 ggtacaccgg tgatgatcag attctggttc gccgtaatgc ggaaatcgcc tttgtggatc
     1921 ttcgcgatct ccagcaggcc ggttttcagc ggacgccccg gataatcaag gatgcgacca
     1981 ttttcgataa acagcgtcag gtgccagtta tcatcaatgc ccttaaccca gccaatacga
     2041 tcgcctcgtc cggtgaactc atatggacgg atcggttcaa atttgatccc cgcgcgacgc
     2101 tccacttccg ctttaaacgt ctcaaccccc acgcgctcca gcgtgtattt ggttttggca
     2161 tttttacgat cggttcggtt accccagtca cgctgagttg tcacgacggc ttcggccacc
     2221 gccagcgtat gctccagcgg cagatagcca aactcgctcg ccgtgcgggc gtaggttttc
     2281 ttgttgccgt gttcgataga aagcccaccg cccaccagca ggttgaagcc taccagcttg
     2341 ccgttttcgg cgattgccac gaagttcatg tcgttggcat gcagatcgat atcgttctgc
     2401 ggcgggatca ctaccgtggt tttgaactta cgcggcaggt aggtctgacc aaggatcggt
     2461 tcttcatcag tagtggcgac tttttcctgg tcgagccaga tctccgcata cgcgcgggta
     2521 cgaggcaaca gatgctcaga aatcttcttc gcccactcgt acgcttccgc gtgcagctgc
     2581 gactcgtaag ggttcgaggt gcagagtacg ttacggttca tgtcgttagc tgtcgccagc
     2641 gcatcaagac cgaccgagtg cagcatctgg tgcaccggtt tgacgttctt tttcagaatg
     2701 ccgtggaact gaaacgtctg gcggttggtc aggcgaatgc tgccatagat ggtgttttca
     2761 ccggcaaatt tgtcgatcgc ctgccactgt ttagtggtaa tcaccccacc cggcagacga
     2821 cagcgaagca gcatcgcgtg gcgcggctcc agcttctgtt cagcacgttc ggcacggatg
     2881 tcgcggtcat cctgctgata catgccgtgg aagcggatca gcaggaagtt gtcgccctta
     2941 aagccgccgg tcagaccgtc gtttaaatct tccgcaatgg tgccgcgcag gtagttgctt
     3001 tcaagcttca tgcgctcggc gtctgtcagt tttccttcga ccactaaagg ccctggatgt
     3061 ttttcgctca ttagtagaca tctcgctgat aacggcgctc tacgcgcagc tcacttaaaa
     3121 attcatccgc cgcttcggtg tccatgccac caaattcggc aatcacttcc agaagtgcct
     3181 gctcaacgtc tttcgccatg cgattagcgt cgccgcagac ataaatgtgg gcaccgtcat
     3241 tgatccagcg ccacagctcc gcgccctgtt cgcgcagttt gtcttgtaca taaacttttt
     3301 ctttttggtc gcgcgaccag gcaagatcga tacgcgtcag cacgccctct ttgacgtagc
     3361 gctgccactc cacctggtag aggaaatctt ccgtaaagtg cgggttgcca aagaacagcc
     3421 agtttttacc cggcgcttcg tcggcggcgc gttgctgcat aaaggcacgg aacggcgcga
     3481 tgccggtacc ggggccaatc ataatcactg gcgtttgcgg gttagcgggc aggcggaaat
     3541 tgtcgttatg ttcgataaat acgcggactt cgccctcttc ttccacgcga tccgcgagga
     3601 agctggaggc accaccggca cgggcgcggc cttccacgtc gtaacgcacc acaccaacgg
     3661 tgacgtgtac ttcgttctcg acttccgcct gcgaggaggc gatggaatac aggcgcgggg
     3721 tcagcgggcg cagcaggtta atcagcgctt cggcatccag ttgcgccgga gagaaacgca
     3781 ccatgtcgac aatcggcgtg gtcgcggcgt aatgctgtaa cttcgcttta tcgcccacca
     3841 gcggcagcag cgtttcgctg cgggtaagcg tggcatagtt ctcaacaatg ttggcggtgt
     3901 tgacggtcag ttcgaagtgc cactgtagcg cttcgttcag aggcaacgtt ttgccctcga
     3961 cggtgacagg ttcatcgcct ttcagccaca gcagttcgac aagttctttc accagtgccg
     4021 ggtcgttctg ataccagaca cccagcgcgt cacccggctg gtaacgcagg cctgaatcgc
     4081 ctaagtcaat ttcgatatga cgaacgtctt tttcagagtt acgcccggta attttctggt
     4141 taaccgaaag gctcgccacc agcggcgcgt ctttgctgta cgggctggtg tggatttcat
     4201 ttaccgcgcc agtagcgacg gattgcgaag gtgccgcgac aggcgcacgc gatttaagcg
     4261 catcaaccac gcgggcgcgc cactcgctgg cagcagcctg gtattcaaca tcggcatcga
     4321 cacggtcgag caggcgttca ccacccagtt ccgccagctt gctgtcgaaa tctttcccgg
     4381 actggcagaa aaattcataa gagctatcgc cgaggctaaa cacggcaaac gcggtgtttt
     4441 ccagctttgg cgcttttttg gagaacagga acttatgcag cgcgacggct tcttccggcg
     4501 gttccccttc cccttgcgtt gacgtcacta cgatgagcag tttttcgctg gcgatttgtt
     4561 tgaatttata gtcgcccgcg ttcaccagct taacgttcag ttttgctgct aataaatcat
     4621 cgcgtaatgc ttcagcaacc cggcgcgcat tgccggtttg cgaggcggag ataatagtta
     4681 tacccggcat ttctgcggct ggcgctggcg tcgctgcaag cgcagcaggc tgctgattga
     4741 gtacgcccca gaaatagcca gaaacccagg caagctgggt aggcgttaaa tcggtcgtgg
     4801 ccgcctgaag gcgtgccagt tgctccgggt tcaacggaag caacgcggaa ggtgggacct
     4861 gtgtcgtcat gcgtcgttat gttccagtaa gcaaagctgt ttctgcgccc tgtcagcgcc
     4921 cataaaacag aagagaagat aaggttaacg gggtgaacgg tgtggattaa agacgggata
     4981 gcgataacta ataaccaaat cgactaacct gttttagcaa tagtctttaa caacaaaata
     5041 gattaaccaa cccaatgaaa aacaaatgaa tttagccaat cattaagata aatcagcgat
     5101 tttgcgcaac aaagtcgttt tagataatgc gaaaaaacag cctttccggt actctacggc
     5161 ggttttatcg tcctgtagag aaattatgat gtccaccacg ttatttaaag atttcacctt
     5221 cgaagccgct caccgcttac cacacgtccc ggaagggcat aaatgtggtc gcctgcacgg
     5281 gcattccttt atggtgcgac tggaaattac cggggaagtc gatccgcata cgggctggat
     5341 tatcgatttc gctgaactga aagcggcgtt taagccaact tatgagcgcc tcgatcacca
     5401 ttatctcaat gatattccag gtctggaaaa cccaaccagc gaagttttag caaaatggat
     5461 ttgggatcag gttaaacccg ttgtgccgct gttaagtgcg gtgatggtaa aagaaacctg
     5521 caccgcaggg tgtatctatc gcggcgaatg ataagagtgt gtcggcggtc aatttccctt
     5581 aagtaacgct atgttagggt gttgtgttct ggatatctgg ggcatgacat ggaagacgac
     5641 tgcgacatta ttattattgg tgccggtatt gcaggcaccg cttgcgcgtt acgctgcgcg
     5701 cgagcgggtt tatccgtttt gttactggaa cgcgctgaaa tccccggcag caaaaatctt
     5761 tccggcgggc ggttatatac ccatgcactc gcggaactcc tcccgcaatt tcatctgacc
     5821 gcgcctcttg aacgacgcat cactcacgaa agcctttccc tgttaacgcc cgatggcgta
     5881 acgacgtttt ccagcttaca gcccggcggt gaatcctgga gtgtattacg tgcacggttc
     5941 gatccgtggc tggttgccga agccgaaaaa gaaggtgtcg aatgcatccc cggagcgacg
     6001 gtggatgcac tgtatgaaga aaacggcaga gtctgtggcg ttatttgtgg tgacgatatt
     6061 ctccgcgccc gttatgtggt gctggcagaa ggtgccaaca gcgtcctggc tgaacgtcac
     6121 gggttagtga ctcgtcctgc tggcgaagcg atggcgttgg ggatcaaaga agtgctgtcg
     6181 ctggaaacat ccgctattga agaacgtttt catctggaga ataacgaagg cgcagcgttg
     6241 ctgttcagcg gcggaatctg tgatgactta cccggcggcg catttcttta tactaatcaa
     6301 caaacgctct cgttagggat tgtttgcccg ctctcttccc ttacgcaaag tcgtgttccg
     6361 gcaagcgagc tgctgactcg ctttaaagcg catccggcag tgcgcccgct tatcaaaaac
     6421 acggaatcac tggagtatgg tgcgcatctg gagccagaag gtggcttgca cagtatgccg
     6481 gtgcaatacg ccggtaacgg ctggctgctg gtgggcgatg cgttgcgcag ttgcgtcaat
     6541 accggaattt ccgtgcgcgg catggatatg gcgctgactg gcgcacaggc ggcggcacaa
     6601 acgctaataa gcgcctgcca gcaccgcgag ccgcaaaatc tgtttgcgct ttatcatcac
     6661 aacgtagagc gcagcctgct atgggatgtt ctacaacgtt atcagcatgt tccggcgctt
     6721 ttgcaacgcc ctggctggta tcgggcgtgg cctgcgttaa tgcaggatat ttcccgcgat
     6781 ttatgggatc agggtgataa acctgttcca ccgctgcgcc agttattctg gcgtcattta
     6841 cgtcgtcatg gcctgtggca tctggcgggc gatgttatca ggagtctgcg atgtctgtag
     6901 cccataatct ctggcgcgtt gctgatgcgc cgcacattgt tccggctgac tccgttgagc
     6961 gtcagatggt acaacggttg attaacgcct gtccggcagg tcttttttcg ctcacaccgg
     7021 aaggtgactt acgcgttgat tatcacggct gcctggagtg tggcacctgc cgtttgctgt
     7081 gcgacgaatc cacactacaa cagtggcgct atccgccttc cggattcggc atcacctacc
     7141 gctttggata aataataagg ataatttatg cccctcttac acctactccg ccagaatccg
     7201 gtgattgctg ccgttaaaga taatgccagc ctgcaactgg ctatcgattc tgaatgccag
     7261 tttatttccg tgttgtacgg caatatctgc accatcagta atatcgttaa aaagattaag
     7321 aacgccggga aatatgcttt tattcatgtt gatttgctgg aaggcgcgtc aaataaagaa
     7381 gtcgttattc agtttttgaa actggtgacc gaggcggacg gcattatcag taccaaagcc
     7441 tcaatgttga aagcggcaag agcggaaggt tttttctgta ttcatcgcct gtttattgtt
     7501 gattcaattt cgtttcacaa cattgataag caagttgcgc aatcgaatcc ggattgtatt
     7561 gagatcctgc caggcggtat gcccaaagtg ctgggctggg tgacagagaa aatccgccaa
     7621 ccgctgattg ccggtgggct ggtgtgcgat gaagaagatg cgcgtaatgc gattaacgcg
     7681 ggtgtcgtgg cgctttccac cacgaatacc ggggtctgga cgttagcgaa aaaattactt
     7741 tgacgggata atcgtaacca attgaatttg gtttgatttg taggccgcac gccacatccg
     7801 acattcagcg cctgatgcga cgcttgacgc gtcttatcag gcctacaagt ccccaggcca
     7861 gaaccgtagg caggatcatc cggcaacacg ttattggcaa tctgcgtgaa tgtttgtcac
     7921 cagtgcctcc agcaccactt tccagtcatc aaccacgcca acatctgcct gtgaaaatac
     7981 cgcagcgctg gcgtcatgat taatcgccac cacaaacttg ctattgcgta cgcccgccat
     8041 taacgctgct gcgccagaag cgccaacaac aatacacacc tcaggtgcca gcagatgtcc
     8101 ggaaatacca atcacttttt cagcatcaac gccaccgttc atcactcgcg cccgactgta
     8161 gcccacttcg gctcccagct tttctgccag catagcgatt tcctggttat ctgcctctcc
     8221 gccctgcccc accaccagca cccgtctggc ctcagctaat gggtcgcgag taacattttt
     8281 cagatcctct gtgctaacca gccagtccgg aagcgcaccc gggacaatgt tcagttgttg
     8341 cattccagag ggcaacgtgg cgtttttcgc tgctcccgcc tgacgcgcca gggaaaggca
     8401 tagcgggcgt ttttctgttt gcagcgtcgc cgttagcgca tttccccagt gggatttgcg
     8461 cacgcttacg gttggaatat ctaacgaagt cacctggcag atgcttgcac catgtaaacg
     8521 ccaggccagc cgggtcgaca gttcatcgcc aaacgttccc ggcggaaaga gaacaacgtc
     8581 cgcaggcgtt cgctgccact gttccaccaa cgcatccagc acctgttccg ccactacagg
     8641 ctgaggttca atctgccagt gcgccagggt acaaccgctg aaatcctgcg ccgccagcca
     8701 gcttgctatt gcggcattct cctgatttat tgtcacaatt gcgatattca tggctgcatc
     8761 ctttggcgta aatagtcctg ccatagcttt tgagcttttt ctgctaccgt ttgcccatca
     8821 atcagcgtcg ctccgcgtcg ttgctctgct cttgccagtt gcagacactg catcgctggc
     8881 atttcggcag caacagtttt acggataatt tctgctttcc ccgcagccat tcgctggcgc
     8941 attcccggta ctggcaaggc cacttcaccg cactgacgca cggcaatcac cgcaggcaag
     9001 cgaacccggc aacaacgcaa cccatgttca gtacgctgtt cgagggtgat aaacagcgcg
     9061 tcgagagtga aacgctccac ctgggtgaag cagggccagc ccagcatttc cgccagcaaa
     9121 aatggcgttt gcccattctg cccttcgctg ctttggcagc cggtaattat cagatccagc
     9181 ggattctgat gctgccattc ggcaatatgg cgagcgacaa attccggcgc aaagcgcaga
     9241 tctgccgccg tctccagcaa tacagcttcc tcaaacccaa gagccatgag atagcgcagc
     9301 cagtgcaatg cccgttcatc ccccatgctc aacgcggtta aagacatcgg cgtaccgttt
     9361 tttctctgcg ccaacagcag cgcggcagcg gcctgttcat cagcaccgag taaacttcgc
     9421 agtagcgaaa tatccggtcc gcttttaccc tgagccgccg cctgccactc tttttccgcc
     9481 agcattccgg catccggttc ggctttaaac gctaacagaa tgttcattcg cacctcctac
     9541 atttcccgcc gccaccagcg ggagggcttt agtttctggt gcccatagcc aggtcacaac
     9601 aaaatcaacc aacaacactg tcgccaggag caataaggtg acttgcattc cccactgcgc
     9661 cagcacccac ggcagcaggc cagtacttac cgccgcgcca agtcgactca tggcggtggc
     9721 aaaaccgacg cccagcgagc gaatgtcagt aggaaaactt tccgcaggca aaatgcccac
     9781 cagattactg actgccgaaa tggtggtgct gaagagaaca aaaagcagca gcgttaatga
     9841 actgccggaa ggcaaacagg ccatcactac cagcgttgcc gccagcagca aaaaacttcc
     9901 cagcaaaaat ttgcgatgtg ccagcaggtg cgtcagaact aatcccagca gcgcgcccac
     9961 aattaacaac gcattaagca tcaggctggc agtcagcgca tcttccagac caatagtctg
    10021 ggcgatagtt ggcagccagg tataaatcac aaaccatggg attacgaggc agacaaagaa
    10081 gacgctgtta aacgccgtgc gccgccagta acgcgaagag aacaaggttt tgatgtgttt
    10141 atgggtcgcc gttaccactt catcgcccag taaaacatgg ggaccaaaat agcgatgcac
    10201 gatagcgtga gcttctgcaa aacgcccctg gcgcagtagc cagcgtggtg attctggcgt
    10261 tccccagcgt aataacgtaa tcaacaacgc gggcagagct gccgatgcca gtagccagcg
    10321 ccaggcctcc gggttttcgg aaataaagtg atgtccggca atacttgcca gcacatagcc
    10381 tacggtccac accacgctga atgcgcccag caaaataccg cgatggcggc gcggggaaaa
    10441 ttcagccagc aaggtgtgac ctactgaata atcgcctccc agaccaatgc caatcaaaat
    10501 gcgcagtcca ataagatgct ctggcgtggt ggcaaaaaat tgtaaaaacg aagcaagcgt
    10561 aatcagcaaa aagctgaagg tgaagatttt ttgccgacca atatggtcgg agatccaccc
    10621 aagaaccagg ctgccaagga acaaaccaag gagcgccgag ccgccgatca ttcccgccat
    10681 aaacggcgtc agttgcatgg cgggcgtaag ctgaataatg gcgtaaccaa tgacgccgag
    10741 aacataaccg tcggtcaggt gtgcgccgaa agtgagcgca gcaatgcggc agtgaaaacg
    10801 gttaagcggt aaatcatcca ttcgcaccgg tgaagtgttc atctttaccc cttcattgtc
    10861 cggtaataag aacggcttct cgcctgagaa gccgaaaagg caacgccccg tcatcgcgga
    10921 acgctgcctg acacattatt tttcaatcgg atagatagta cccgtattca taatgccatt
    10981 aggatcgaac tgctttttca gcccttccag caacgcccac gcgctgccgt gttccagttt
    11041 gctccagtga acgcgatgtt taccgatacc gtggtggtgc accatcgaac cgccgaggcg
    11101 aatggtttct tcacagatga tcttgttgag cggattgtgg tacttgtcga tttcctcttc
    11161 cggcttacag tcaacgacgt tgtaatcgta gacgaagtac atgttggtgc cgttctgata
    11221 gctatgagag gaatgaccgc ccagcatggt gatgtcgtcg gcgtgcggga actcagtacg
    11281 aatacggtta ataacgcttt cgtagatttc gtggatgcag ctccagcagc cggacacttc
    11341 agtggtaaag cccatgttgc cggttttgag gatctgcaca cgttcggcag ccactttatc
    11401 cggtccccag ttcaggttgt taaaccaggt ttcgatcagc ttgctgtcca cgcgctggca
    11461 ttgcgggtag cgggcaacga tttccgcaat cccttcgccc gtcgccttcg caatgcgagg
    11521 gttaccttca gccataaaga tcagcacgca ttttccgtct gcaaaatggg tgaagtgttg
    11581 ggtgccatct tcagcgtcat acaaacgagc gatcgacgga cgataccctt ccaccatgac
    11641 ttcacgcagg atgttgaagt cggttttcat gtcttccagg atatagccgt agaagaggtt
    11701 gttttccggg gtgaatttaa agattttcac tgttacttca gtgatatagc aaagtgcacc
    11761 ttcgttaccg atgatgatgt ggcgaatgtc cgggccagcc gcgcggcgtg gcacgttttt
    11821 aatgcgtgtg acggtgccat cagccaatac tgcttccaga ccaacgacca tatcttcgat
    11881 tgcgccgtag agtgtggaga actgcccgat actgcgggtt gctaccaagc cgcccatctg
    11941 cgccagcggc tttgactgcg gagaatgccc cgtggtgtaa cctttttcac gcaacgcatt
    12001 ttccagcacc tccagcggaa ccccacattg cgccgtcgcc tgcatattct caatatcaat
    12061 attaatgatt tgattcatgg cggagccgtc aagcaccacc gagttttcca caacggtttc
    12121 cagcccacct tcagtggcgg aagcgccggt acgcggcaca ccgttaattt tgtgcgcatt
    12181 cataaaattc agcacacggg atacttgctc tgtggaaccg agttttacga ccgctgccgg
    12241 aatcggcaaa gtataaatgc catgaatatc cggaaattta cgaaaacggt caatactgtt
    12301 cttctttaat actgtttcat cggtaattac gcgatctgca ccaacaattt cctttagttg
    12361 gtcgacaatc gctgcgcgag ataaagacat agtaattcct tcctgataat aaaaatggaa
    12421 taatcattaa taagagaaaa cagaaaccac cgttatggat tagcgcacta aataaccacc
    12481 attgaccact aataaatgcc cattgacata atttgaagcc ggacttgcga ggaatacagc
    12541 tgcgcccatt aaatcctgag tatcgcccca acggtttgcc ggaatatgat caagaacgcg
    12601 ctgattggtt tctggattac tgcgtgtcgc cagcgtaata tctgttgcat aatagccagg
    12661 agcaatacca tttacctgaa tattatattg acctagttca tcacaataag ctttggtgaa
    12721 tccggcaaga gcatgtttag tggcagaata tgcaggtgac cattgtccac ctaagtaaga
    12781 gaacaatgaa cagatattaa taattttgcc gcttttctgc gggatcataa tttttgcagc
    12841 ttcatagctt aactcgaatg cggcggtcag gttcacatca atcatcggat cccagtcggc
    12901 acgaccgaag tccagcacct tattcagctt acaaataccg gcattgttta ccagaatatc
    12961 aactgtaccg aaacgctcac agcaagcagc gataatcttc tgcggcgcgc cttctgcggt
    13021 gatatccacc tgcatgaagt ccacctcaac accctgtttt tcaatcattt cctttgtttc
    13081 gccgttatct ttcaatcatt tcctttgttt cgccgttatc tttgacgaaa ctaggaataa
    13141 agatatttgc gccagctttg gccaacgcca tggcaaatgc ctggcctaaa ccgctattcc
    13201 caccggtaac aattgcggtt ttacctttca gtgagaaaaa atccattgag aacgcattga
    13261 gagattcgat tgacatagtt agctccaatt tccgtaaggc aaaaaaaaga aaggtaactt
    13321 cccccgcgag agggaagtta cctttctcat tatctctggt aactggagat aatttttagc
    13381 ataacggcat aactgctaat gtgatcgtaa tcacagtgtg atattcgtat tgtcaaatag
    13441 ttttaaaaag cagaagcgtg attactctca cacaacacct ctgctttcca tgttatttct
    13501 tattgcaatt actagagacc tgagaaaagt aatccacctg aaagggcggc ttacttttct
    13561 cttttttttg tctattacaa atgggaatac gatatgcaac acaactcata tcgccgttgg
    13621 ataaccctcg cgataattag ttttagcggc ggcgttagtt ttgacctggc ttatttacgt
    13681 tatatttttc aaattcccat ggcgaaattt atgggattca gcaataccga gataggttta
    13741 ataatgagta cctttggtat tgcggctatt attctttatg cccccagcgg cgttattgcc
    13801 gataaatttt cacaccgcaa aatgattact tccgcgatga tcattaccgg attactgggt
    13861 ctgttaatgg caacgtatcc accgctgtgg gtaatgctct gtattcaggt cgcctttgcg
    13921 ataacgacga ttttaatgct gtggtcggtg tcgattaaag ccgcctcgtt gctgggcgat
    13981 catagtgagc aagggaaaat tatgggctgg atggaagggc tgcgcggcgt cggtgtaatg
    14041 tcgctggcgg tgtttaccat gtgggtcttt tctcgctttg caccggatga cagcgccagc
    14101 ctgaaaacag tcattatcat ctacagtgtg gtttacatct tgttggggat tctgtgctgg
    14161 ttttttgtta gcgataacaa caacctgcgc agtgccaata acgaagaaaa acagtcattc
    14221 cagcttagcg acatccttgc cgttttgcgt atcagcacca cctggtattg cagcatggtg
    14281 atttttggtg tcttcacaat ctacgccatt ctgagttact ccaccaacta tctgaccgaa
    14341 atgtacggca tgtcgctggt ggcagcgagc tacatgggga ttgtgatcaa caaaatcttc
    14401 cgcgcgctgt gcggcccact tggaggcatt atcaccacct acagtaaagt gaaatcccct
    14461 acccgcgtga ttcaaatcct ttccatcatc ggtttgctgg cgttgacagc cctgctcgtc
    14521 acgaactcta acccccaatc ggtcgcgatg gggattggcc tgattttact gctgggattc
    14581 acctgttacg cctcacgcgg gctgtactgg gcctgccccg gcgaagcgag aacaccgtct
    14641 tacattatgg gcaccacggt aggtatttgt tcggtgattg gattcctgcc ggatgtcttc
    14701 gtttacccga ttatcggcca ctggcaagac accctgcccg ctgcagaagc ctaccgcaat
    14761 atgtggctga tgggcatggc ggcgctcggc atggtgattg tctttacctt tttgctgttc
    14821 caaaaaattc gtactgctga tagcgccccc gcaatagcta gcagcaagta agccaggaaa
    14881 attacaggag aaaaatcatg tcgaaaaaat acatcatagg gattgatggc ggaagtcaga
    14941 gcacaaaagt ggtgatgtac gatctggaag gtaatgtggt ttgcgaaggt aaaggcttat
    15001 tacagccgat gcacacgcct gatgccgata ctgcagaaca tcctgacgac gatttatggg
    15061 catcattatg ttttgcaggt cacgatttga tgagccagtt tgccgggaat aaagaagata
    15121 ttgttggcat cggcctgggc tccatccgtt gctgccgtgc gttattgaaa gccgacggca
    15181 cgccagctgc gccgttgatt agctggcagg atgcacgcgt tacacgccct tacgaacata
    15241 cgaatcctga cgtggcgtat gtaacctctt tttcgggtta tctgacgcat cgcttaaccg
    15301 gcgaatttaa agataatatc gccaactatt ttggtcagtg gccggtggat tataagacct
    15361 gggcatggag cgaagatgcc gcagtcatgg agaagtttaa tatcccccgt catatgctgt
    15421 ttgatgtgca aatgccaggc actgttcttg ggcatatcac acctcaagcc gcgctggcga
    15481 cgcatttccc ggcaggactg ccggttgttt gtaccaccag tgataaaccg gtagaagctc
    15541 tgggagccgg attactggat gatgaaactg cggtaatttc tcttggcact tacatcgcat
    15601 tgatgatgaa cggcaaagcc ttgccgaaag atccggtggc gtactggccg attatgtctt
    15661 ctattccgca aacattgctg tatgaaggtt acggtattcg caaaggcatg tggacggtga
    15721 gctggctgcg cgacatgtta ggcgagtcgt taattcagga tgccagggcg caggatcttt
    15781 caccggaaga tttactcaac aagaaagcct ctagtgtgcc acctggctgt aatgggctga
    15841 tgacggtgct ggactggctg accaatccgt gggaaccgta caaacgcggg attatgatcg
    15901 gctttgattc cagcatggat tacgcatgga tatatcgttc gatattggaa agcgtggcgc
    15961 tgacgctgaa gaacaattac gacaatatgt gtaatgaaat gaatcacttt gcgaaacatg
    16021 taatcattac tggcggcggt tcgaacagga tctgtttatg cagatttttg ccgacgtgtt
    16081 caaccttccg gcacgccgta acgccattaa cggttgtgca agcctggggg cagcgattaa
    16141 tacaggggta ggtctggggc tatacccgga ttacgcaacg gctgtcgata aaatggttcg
    16201 cgtgaaagat atctttatac cgattgagag caatgccaaa cgctacgacg cgatgaataa
    16261 aggcattttc aaagacctaa ccaaacatac tgatgtgatc ctgaaaaaat cgtatgaagt
    16321 gatgcatggg gaattgggga attggggaat gcggattcga tccagagctg gtcgaatgcg
    16381 tgaataaggt aggccgggcg gaaaactgcc cggcctgaac atacctgaat ggttatcccc
    16441 gctggcgcgg ggaacataag ttcgacaact taaaaaatga taacaagtcc ggtttatccc
    16501 cgctgatgca gggaacataa taaaaacttt aactagttcg gtttatccct gtttttacaa
    16561 ggaat
//
LOCUS       DAZPJR010000084        16555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPJR010000084 DAZPJR010000000
VERSION     DAZPJR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.085"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHVB7_002551"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHVB7_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR1914505.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="ACHVB7_002552"
     CDS             complement(1899..2636)
                     /locus_tag="ACHVB7_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914506.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHVB7_002553"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHVB7_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR1914507.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3584..4093)
                     /gene="traS"
                     /locus_tag="ACHVB7_002554"
     CDS             complement(3584..4093)
                     /gene="traS"
                     /locus_tag="ACHVB7_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFR1914508.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(4090..6915)
                     /gene="traG"
                     /locus_tag="ACHVB7_002555"
     CDS             complement(4090..6915)
                     /gene="traG"
                     /locus_tag="ACHVB7_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR1914509.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(6912..8285)
                     /gene="traH"
                     /locus_tag="ACHVB7_002556"
     CDS             complement(6912..8285)
                     /gene="traH"
                     /locus_tag="ACHVB7_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR1914510.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(8272..8664)
                     /gene="trbF"
                     /locus_tag="ACHVB7_002557"
     CDS             complement(8272..8664)
                     /gene="trbF"
                     /locus_tag="ACHVB7_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR1914511.1"
                     /translation="MKEKINDTEPGIKQIEREIERGCDNAKKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="ACHVB7_002558"
     CDS             complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="ACHVB7_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR1914512.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="ACHVB7_002559"
     CDS             complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="ACHVB7_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR1914513.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="ACHVB7_002560"
     CDS             complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="ACHVB7_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR1914514.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9819..10154
                     /locus_tag="ACHVB7_002561"
     CDS             9819..10154
                     /locus_tag="ACHVB7_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR1914515.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="ACHVB7_002562"
     CDS             complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="ACHVB7_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR1914516.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10490..11233)
                     /gene="traF"
                     /locus_tag="ACHVB7_002563"
     CDS             complement(10490..11233)
                     /gene="traF"
                     /locus_tag="ACHVB7_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR1914517.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11226..11483)
                     /locus_tag="ACHVB7_002564"
     CDS             complement(11226..11483)
                     /locus_tag="ACHVB7_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR1914518.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11510..13414)
                     /gene="traN"
                     /locus_tag="ACHVB7_002565"
     CDS             complement(11510..13414)
                     /gene="traN"
                     /locus_tag="ACHVB7_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR1914519.1"
                     /translation="MKEKINTFFRSLNFNYSVKPCNLCAIVFFLSFSVFADSNSDYRA
                     GSDFAHQIKGQGSSSIQGFKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEF
                     TNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPV
                     TGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLT
                     GSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGTCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQ
                     IADQLKQAGQ"
     gene            complement(13650..14030)
                     /locus_tag="ACHVB7_002566"
     CDS             complement(13650..14030)
                     /locus_tag="ACHVB7_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914520.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="ACHVB7_002567"
     CDS             complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="ACHVB7_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR1914521.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(14674..14979)
                     /locus_tag="ACHVB7_002568"
     CDS             complement(14674..14979)
                     /locus_tag="ACHVB7_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914522.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(15009..16001)
                     /gene="traU"
                     /locus_tag="ACHVB7_002569"
     CDS             complement(15009..16001)
                     /gene="traU"
                     /locus_tag="ACHVB7_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR1914523.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15998..>16555)
                     /gene="traW"
                     /locus_tag="ACHVB7_002570"
     CDS             complement(15998..>16555)
                     /gene="traW"
                     /locus_tag="ACHVB7_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR1914524.1"
                     /translation="DLWPVKEPDMLTVIMQRLTALEQSGEMGRKMDAFKERVIRNSLR
                     PPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEGRVFARQGEVMNPLQYVPFNQTLYFI
                     NGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKSLDSRVYFDQNGVLCQRLGIDQV
                     PARVSAVPGDRFLKVEFIPAEEGRK"
BASE COUNT         4156 a         4443 c         3779 g         4177 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gtgagagttt
      181 tactgccgga tacggtccgg gcagagtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catctcctta ccggtcgata ccccgtcacg
      301 cacggggtca gcaccgtagg aatactgcag actggctttc aggtgctctt tctcaccaat
      361 ttcaccggca gcaaactctg caatctgatg gctcggggag cggaaaaagg cacgggtatt
      421 cagcacatca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagcctcc gaacttacgg gcttccggca ggatctccac
      541 caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac
      661 cggcttcagg gaggcatgag tgtcggcatt tgacgagata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg tatggtgaag ggttcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttcg attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcagatacg ccgcttccgc aaaaatggta cgtcctgatc cctgccagaa
     1021 cgggtcctct ttcgttccca tcgggatcag cgtgttcgcg acattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccaggc ggcacagcgg gcatccagcg gattcaggat
     1141 cttatcgatg gagggatcat agtaactttt aacaaactca cacgaacggt cataaatcac
     1201 caccatgtcg ccacgtttac gggcatagtt ggcaagacgt cgaataacct ctgacttacc
     1261 cgtcgacact gttccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 atcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     1501 tgtagccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttgcaggc
     2581 ttctcagtaa attgattatt ctcaccaata tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagct ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac cggatgtgcc
     3001 ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccagca gcaaggccca caccgagtgt ggcaccggca gaatttgagt tatagccggt
     3181 aataccggca cctaacgctg caccaacagc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctgcatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggtttaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcatttttt ttgttttcat ccagaatatc cttttaacta acagactctt
     3601 ttttgaacag acctattact gagactattg cagagagatt ataacgattt atatctatag
     3661 tataaataaa ggaaataatt acaaacagag ttattgataa aaacccaaat aagaacgtat
     3721 gagcaaagga aactattgaa aaaacaatca caaacgacaa gatataagtt ttgttcttat
     3781 catacataaa agatataact ttcgactcat ctctgaattt tttagggatg gcaagataca
     3841 acatcattcc attagtaatg gcaacaaaaa ggataaaacc cattacgcca ctaaaacata
     3901 tatcaaatcc agcatctcgg atatcaactt tatctgccag caaacgaaga aatgtcggcc
     3961 ataataaaac ccataaaaac acatacaccc ctggttttat gacttgtaat aatggcggaa
     4021 tattaactcc ctctacagag agtacatcct ttagtttctg tgtctccgtt tttatatcat
     4081 agtgagtgat cataatggcc ccttcagtct atccttttta tcatcaacca tttttttcac
     4141 agcctcttct gatggctcag gtattatctt ttgactttct cgctcttcat tatatttatt
     4201 attctgagat aatgcctctg ttttatgatg attttgcagt tcagaatact gccctcttac
     4261 aatattttcc tcgccacgga tactgttctg agtatctccg atattgccac gatattctgt
     4321 gaccatatta tcaacctgat gttttacatc attacggata ttactgtcct gcgttctttg
     4381 ctcaataatg gcctgatgtc cctgatggtc agcaataata tcctgactgc caccacccga
     4441 aggtacgctc tccattcctt taccaatatc gccacgggat tcccgccagg cattatcaac
     4501 accaggctgt acctgttcct gcacaaaaaa ccaggccatc gcccgacgac gttctgcaat
     4561 ctccggcgaa ctggtatttg tcagaatagc ttctgcatcc tgcggtgcgc gtttcatcac
     4621 atactgtgca aactgttgcg acaggtcttc actcatctgc ccgctcatgc tttcagtacg
     4681 ggaggccatt tccgcatatt catggctgcg ggtcatattc gtcgtgtact ggtcgtaact
     4741 ctgtttcgct gagttcaggg ctgcagacaa ctggtccacc cgggagtcag cattattgtc
     4801 agtatggctg ccggactcac tgaccttgcg actggtaaag taatcgctgg cttctttaaa
     4861 gtccttactg gctttagcgt caatatcatg gcgagcatca tgggtagctc tgctgccact
     4921 acttgcagaa tgtgaatcat catccgttcg atcaagactt cccttaaacc cgcctctgac
     4981 accaacccca ccaccaacac caagaattcc aggtttaatc ccccattcag catgagcatc
     5041 tgcatatccg ccatacgaga acttagaacc tgttgatgcc agttcctgag tcgcctgctc
     5101 attactgata ttatgcgcct tcgcatagct ttccactgca ctgcgcatac ggctggccat
     5161 catggagtcc tgtgcactca tggtgctgtc agcgccaccg gtgacagaat cactgctccc
     5221 ccggttagaa ccaaactgac tgagcgtgtt ccaggcactg gcaatactgc tgctgaaacc
     5281 atgcagggca ctttctgctc tgttcgacgc ctcccgggcc atttcctgtt gcgccgctgc
     5341 aatctgacgc gttgcattga taccgaccgg taaacgggac atcgctccgc ttgcatccat
     5401 caccatatta ccgtcacggg tctgtgtggc agttgcgcca ctgccggtct ggtacatcat
     5461 ctgaccaaac gacgtggtgc tgttggtgct ccagctgaag ccgttcacgt tttccgtctg
     5521 catgttgccg taggagtaat taccgtcaac cacactgcct gctgcactgg ccgtcggact
     5581 gatagaggaa gaggcgaagt ggctgtacac gctggaaaaa cctgcgccaa ggccttttac
     5641 catcatccat gacaacgggg ggatcatggc tgaaagatac ccggcagtag aggccaggtc
     5701 agagtatttc agctgtatct gagagagttc agacagcacg accggcgcac cattcatctt
     5761 cgcatagaat gtcatggcac tgttcagaat ggcatacaac aacggccagg tctgcagcca
     5821 catcagggca aacacatagc ctttcaggac ggacagtgtc agtttgttga agacagctgc
     5881 cagtaccaga agcgggaaaa taccaatggc aattcccgtc aggatagtct gtaccattgg
     5941 caggtttcgc attgtcacat gaccgataga gacatgcgcc agacgttgct tctccatcga
     6001 tgacgtggtg gccagattca ccaggctggc ggtgtcacca ttacgggcag cattactggt
     6061 gataccctct ttcagggcat ttattgtgac gttctggcgc ataatctggc tggcagactg
     6121 actggagccg tagaaataac tgtaactgtc actgaccagt tgtctgaata acaggtccgg
     6181 gtccggtctg ccgccaaata tctgctgcac ataataatgc caggtcttgc ctcccgtctt
     6241 tgtatcgaga ttcagcctgt ctttcagcgt gaccgacgca tccttacagg ttatgaaatt
     6301 attattgttg tcataaacgc cccgtagcgg gctgggtcgg gaaaaaatca gcgtgtaggg
     6361 gtcagcggag gccatcagat cttccagcgt gtatttatga ttcaggtaaa tatcacccag
     6421 cacgcagttc tggacgtagt cctggaacag attgatgatt tccggattcc gggacaggaa
     6481 atcggtgctt ttcacaatca gattcgcccc gaacagcatg cctgtcttgc tgtaggtgac
     6541 gctgtccggt tgggtgaata tcatctcgta gctgaccacc atcgcatgcc cgatacgggt
     6601 cgtaagagaa agtggcatcg ccagaccgac cggaacatta tccacccgat ggactttgac
     6661 caggtcactg ttatcaataa tctgcaccga tgtgcggaca ttaaccagca ggctgataag
     6721 cacaaacacg gccacccagc ccagcaaatc catcacgtta tgccgctgta cccacataaa
     6781 agccacagca acaacagaca gggtgagcgc gattttttca attgaatccc aggtccgggt
     6841 actcataaat gcagcaatgg cgttcaggtt attccgcaac cactcaccac cggcaatcac
     6901 ataaacttca ttcacagtga attccctccg aagtgatagt tgttctggta acggctgagc
     6961 atacgggcgg aaagctgctg acgcatgtag ttcatctgac gttcgacaac cagcagcgca
     7021 tcctgctgga cctgtacctg actctggaaa atggcaatct gttgctgtgc ctgattcaga
     7081 ttctccagaa cattgtccat cacagcctgc ggatagttcc cggtggcaat catggcccgg
     7141 gcctgctgaa gcagttcctg aatgtactgg agcatgatgt cgtagccgat atagtccgtc
     7201 agctggtaaa cgacgctgtt ggacacaccg agcatctgcg ggtcaatcag atatttaaaa
     7261 acgggaatgg tggtgctgga aataaacccc tgttcccggg cgtcaagcgg agtgtcactc
     7321 actgctttat tctgaatact ggtgagcagt tttgtaatct gtgacttcag ggctttatcc
     7381 cgggcgatgg tcacatttgc atccgccacc accttcagac acttgtccga atcgttgcag
     7441 tggtaaattt ttgccgttcc cccctccata atcgcccgga ggatatcgcg gtcagtggta
     7501 cgcgccggca ggggggtaat ctcgctgtct tccccaaaaa tcagcgtacc ggtgagcgtc
     7561 atgataaatt ctttcaactc tttgttgccg tcaaacattc tgtttttttt cagcgcctcc
     7621 catatgatat tctggttttt cagcacccgc tccttgtcct tgtcgctggc tttgtcacga
     7681 acagatgagg actgccctcc gaccgtgcac ccctgacgag atgcggccca gtcggcgaag
     7741 atattgcttt cactgccaat gtcctgacaa actttctgct gagattcctg cgtctgaggg
     7801 aacagccccc ccacaagccc ctgtgccacc tgacaggaac tgaggttcat actgttaatg
     7861 tcacttgcca ttttctgcag gaagtctttt gcggttttta tttccggcac cgttgtctgc
     7921 agggcaaggt caaaaaagta accggcagca ttgctcataa tctgcttaac aaaacgctgc
     7981 aactgatcgc cattaataaa actgaacgag ccgagatagg cgtcgatgcc gccacatccg
     8041 gcattgatat ccggcagcgt catggaaatc agctggatat ttttgacctg ggtgcgggca
     8101 tacagggagc caccataggc atacccggcg gcctgcccct gccagacgcc cggctgtgtg
     8161 gtattggagg cgaagcccag cttgttaaag aactgattca tatcgctgtt cacatccgct
     8221 gacgctgcgg gtgtgaccgt cagtaaagcg gcacaaagaa ccagaagagg tttaatgcga
     8281 agcattattg ctgccctccc ttaatgtgtt ctcccggcgc atacggcgtt tcagcaggaa
     8341 tatccggaca cccccaaata ttatttcgca caacggcgac agagaagccg cagcaagaaa
     8401 accagctgca agcgcatata acataatcgg aatgacatac atcagggtgt tccacatata
     8461 acgaaaatta tttaattccg ggtcagcttt ccacgaatca ataccggcac tgaaaaaatc
     8521 atgcataacg ttccttacaa tcaatccggc agcaaagaaa accacaaaaa gccagaagta
     8581 tttttttgca ttatcacaac cacgttcaat ctccctttca atttgtttga ttcccggttc
     8641 tgtatcattt attttttctt tcatcataag ttccctctga tatttctgat gattcatgca
     8701 gacaggtcat gcggctgtaa tatatttcag acaccgaaat ccatcccaca attgccccca
     8761 caaaaaacac aacagaaaca gaaaaggtaa gacacagcga aacgacaatt tccacagggc
     8821 tggcgggata aaaaagcccc tgacggataa aggggatatt caggctgacg attattcccg
     8881 tcgcgatgcc ggatattccg gcaatgagta acaccacctg cttatttcgc acctttttcc
     8941 tctccgtata tctgcagaac ggtatccatc cgcgccataa aactggcggc atccgttgcc
     9001 ccctgcaaaa gcggtaatgc ctcaagtgtg ttgacgttga ccaggaaggt ggtcggtgtg
     9061 gccaccggga tattcgggaa gaaggtctgc atcacgtccg gtggcaccgg taatgcttcc
     9121 ggaaaggcag tatccccctg accatccagg gtgtagggaa aaacggaaaa gccgtactgc
     9181 tgtgccagct gcttcagtac cgggtcaaac tggtgacagt aagggcaatg cccctgcata
     9241 aacagcacca ccttccagtc ggccaggtta acctgcctgc cgttgctgag gcggaaccag
     9301 cggggagccg gcttttctgt tctggctgat gtgtctgctg ccggttgtgc aggctgcacg
     9361 gccattccct gcggattcca gagtcgttca atttcatccc gggtggaggc ctgcacacct
     9421 gtggccatca tcaacaaaat gaccggcagt aatttagtga gagacatgtc cgccctcctg
     9481 ctgcccttcc atcatggcct gttgcctggc ttccagtgcc tcacgctctt ccaggctgac
     9541 acgtgcaatc catgcatcca gcacctggta tgcgccgaac agcacaagga tggccgcaat
     9601 caactccgcc agaaaaaaag ccatccaggg tttgctgtaa acaagacggg cgacgatatg
     9661 aaaccagaca cccagggaaa atacccacat tcccgtaata tcaagccggg gtaaagagaa
     9721 tctgcgttta cttatcatgt taaactccgg ttattattta catgagagta ttcatgtctg
     9781 taatattcgc gtttgtgtga tattaatcag aggtacaaat gaaaaagtta tcttttaaag
     9841 aaaaactgga aatagtgaga aacatcataa gagagtcatt atcaggtaat gcggcttttg
     9901 ctttactggt gtattcatta ttgcactccc tccccattaa aacctttgct gatttatttg
     9961 tgacgatact gttatttata gcgattatcg ctgttgtgat atggctattt ctaatcatct
    10021 atatttattt ttgtgagtta ttcagaagtc actggatagc agtctggttt atcctctggt
    10081 ctgtggtaat caacctcact attggatttg gcatttatga caaattagca ccagtcattt
    10141 cactaatgaa ataataaaaa accacatcac tccagaccat atggctgaca acataataca
    10201 aacaaacatc cttctgaaac tgtcaaactt cgtgataacc ggtatggtac tgactgccat
    10261 tgaaacaacc ggtacggcac actgattcat aaccgcacca caaagaaaca agaaattatc
    10321 tggcagcata catacactta tgacatttac tacaaagaag acaaacgagc tcagcagata
    10381 aactccttta ttatttggtc ctgtaaagaa ataaccaaca acccccacga taaacaggct
    10441 ggcacctgta aaaaaacaca aatcccctgt atgcatatat aacctttggt taaaaattcg
    10501 gtttaaaatc ttcagaaacg ttcaggaact gttttgccag gtcgtcctgt gaaataaagc
    10561 cgtatgataa cgggcgaaca ctgccctgtt tcgggtcaac cagcatcatg gccgggaaat
    10621 atttcacacc gaggcgctgc gcctgcccct ggtcagtccg ggaatccggc aacagcggat
    10681 taatcacgcc atccacggaa acgggaataa cactcagacc atacgtatcc cggaagccat
    10741 taatgacctg cgccagttgc ccgtcgatgg ggtcctgccc ccggtaaaaa aacatgatgc
    10801 cgtagtgttc agccagtttc gcaatggcct gtcgctgctg cgcctggtct gccgccagct
    10861 ggttccgtac cgtgccgttg taatggctgt actgcaggtt atagtccagt tcgggatgtg
    10921 ccagcatggc ctttttagca ctcatggtga aaagcccggc ctgctgagtc cagtaattct
    10981 gcagccggaa atatttaaca aaattatcca cgccgggata cagaatggct tcgtacagcg
    11041 cccgctttgt tgccgtctgc agcgcggcca gtttctgcat aatatccggc tcctgacggg
    11101 gggctgcagg tacaggttta ttttcttttt ccttcggatt tattttctcg ttataccact
    11161 gccagcctgc atctttccct gacgccggaa aaataaagca gcagagtaac agtggcagta
    11221 atgctttatt catcttcatt cctttcatca ttttttcttt catcatcttt ctgcaggcca
    11281 gtcagggcag aaaaacaaaa atacagaata aacaggagca caccggagta aaggataacg
    11341 aaagcagcag caagtatccc gtctgccgta gtttctttta ccgtatccca cgaccagata
    11401 attaccggag aaatgacaat cgctgtaatc gaaagccgga taagaaaatc aacaagacga
    11461 ttagaagtga atataacttt catcatttcc cccggttatt ttccatacct tactgtcctg
    11521 cctgtttcag ctggtcagca atctgctctt tcactttctg cgtcagaacg ccactgtccg
    11581 ggattttctg gttattcatc aggtcttcgt agaagttagt gaaatccagt ctgtcgaact
    11641 gaattttctg cagctcatca accgtaatcc cccgacagtc aggatgcttc gcactgccaa
    11701 acccgatacg caactgcccg ttacgcccct gctgctggac tatctgcgcc agcttcgaat
    11761 caaactggca gtagctgcgc tttttctgca gacagacacc cagcactttc cgggagcaga
    11821 actcgcccac actcaccgtc agtttgttgt cctttgcctt tcccagcgcc ttttcttcac
    11881 tgttgcactt cgccagcccc acatcctgac cccagccgcc atccttacag cagttactga
    11941 agccggctgc ggccttacgg cagaatttcg cctcacccgt gaaagccctg acatccacac
    12001 cattcagggc ggcaacatcc ttacctgcgg cggcaagcgc agccagctgt gatactgctt
    12061 caccaaaatc actgtttttt ccgctctgtg ccttatcgca ttcaccgtca aggcagaaga
    12121 catcaccacc acagaccatc acttttcctg atgttctgga ctcgcaggag tacgtggcgt
    12181 actcatgcag acaggttccc tcatcggaat aaaacgcgca ctgacgtgag gccagcgtac
    12241 aggccggatt atcgacatat ttctgacagg tgccgttatc tgcggactga gtgacatatt
    12301 tatcccggta tgcccagcat gcctgtgtga tgttccaggg tttaccttcc atcactccgg
    12361 ttttcgtacc acccggctct gaacactcag tgcctgtcag cacgccatcc gctttattaa
    12421 acggacaact ctccacccac tccacccggg gaacccattc tttatcacgg accttcatcc
    12481 tgagtttcag cgtaaaggtg gaagcaccac tgacaagcga ttcatagact ttcctgtcac
    12541 cattcccgtg cgggaggcaa ttaccgttag cagtacagcc actaccggtc agaacctgcc
    12601 cctgtgtcac ctgcaaacct gtcgccccgg acagggggta agtatccgac tgcccgtttg
    12661 ccaggccgac attaaaggtt gtattcatga aggtataacg ggagttcagg aagaaaaaag
    12721 cggcgtatac agacaatgat gcactttcca ctgtaccggt taccggagcc gtgacactga
    12781 acaccagttt cagcccgttc atactgaacc ggaactgtga gtgcggtatc gtcacttcac
    12841 gatattcatc cgtgtatttc cagtccccgg tgatactggc agtccgtgtg cagtactcct
    12901 ccaccatcgt gtcccgctca caggtatagt ttgtgaattc actgcggttt atctcctgcg
    12961 cactgcactg ctgtccggta tttcccacaa tgctgtcagc cctgttcacc acatccctgc
    13021 cggtctgaat gaacggtgca tccggtgaca gaatgtcttt cggcttgttc atgaaggact
    13081 ctgtgatggt ttttccggtt tcaccggtcg cccattccgt ggtgccgtca tttttcaggc
    13141 cgccatcccc gccggcagtc acgccaccgt aatattttgt ctcgtccgga ttcgcgttat
    13201 agccggggat actctcctgt ggcttaaagc cctgaatact gctgcttccc tgccctttga
    13261 tctggtgagc aaaatcagaa ccggcccggt aatcactgtt gctgtcagca aacacagaaa
    13321 agctcagaaa aaaaacaatt gcacaaagat tgcacggctt tacggaataa ttaaaattca
    13381 gagatctgaa aaaggtattt atcttttctt tcataacctg aaccatactg gagaatgaag
    13441 tgatgtcatc tcaacttcag aactgtgttg attgtggtaa agcattaagt cgaacagcca
    13501 caacgtgcgg gaattgcagc tcaacggacc cttttggaaa aaaacggcgc agcgatcgct
    13561 tttaccagtg gtttgtaggg ctgtttatta cggctattat tattgctggc ggattatggt
    13621 acatggggct tcttgacgta ccgggattac tactcagata acgacttcag taacgatatc
    13681 agtgttctga aatcggcaca taacgagtga cgacaatacc ggttatacca gtaatgaggg
    13741 gtgtcgggct gaagcctgaa acagagctga aacttactgt taaaagcccc cttcaccgac
    13801 catccccatg ccagacgtgg tattgctgat tcagcatcct gccgcagtcg ctgctcaaga
    13861 agggcagcca tttttaccgg acaggcaaag gcaatataag gaggcagctt tgtaacaggg
    13921 aaaccatgtc cctgacagga agcatatgtg cggaagccgt gaatgttcat cgcggccacc
    13981 agggcacgga tctccggatc aatctgataa tacccggcag gtaaagtcat ttttgtttat
    14041 ctcccgggtt attcagcaac gcccgagcca cctgccggca gtcccccgtg gcggccactt
    14101 tctccagcgc ctggccgacc cgcaggttac ctcggataat gtcgtatccc tgactgcaga
    14161 acaccaccag tgccggtaca ctgcgaatgc cgtactgtga aaacagcgtc gggtcgatct
    14221 gaacaccatc agtcgcgccg tctttcacca gggacagcac ggcttcagcg gtggtcttca
    14281 ggtcattatt caccatgccc cgcagtgtgg cgggtatacc gtagtgccgg gtttcaccca
    14341 gcatccgttt cagcccctct tcgggaatgg aaaacgacac aaaatacagg gcaccctgcc
    14401 ggggtttccc gtcctgcgaa gcctgctgtt tacgaaccag ctcatccaga aaatggttat
    14461 ctgaacgctg aagggggttt tccagcacct gtttctccgc ccaggctttc agctgatggt
    14521 caggtttctc tcgcagttgt ctgcttaaat tttcctgctg cttcaggaac tggcggtttt
    14581 caggtgtgtt cacattttct gaagcatgaa cagcaagatg catccctgct gtcagagcaa
    14641 aaagtgcaaa tattttccgg ttcatgatat ttctcacttt ctgctattta caggcatgaa
    14701 ctcatcagaa aaaagataat tgagaaaaac agccataaac gcacatgtaa atgtcacctt
    14761 cagatataat ctgacaagtg tcacttgttc tgatatatct gccttcttcc attcatgaat
    14821 aatttcagcg agaaaactgt caggtgcaaa ataaaataat ataacgggca ccattacaag
    14881 accaaaagga aataatttta atgttctgct tatcagcctg agcagcacag cacttatttt
    14941 cctgaggtgc tgaatatatt tactgtccat tgttgccatt ttcatttcct cttattcatt
    15001 aaaatgattt acaggaatac acagttacgt ttacgccaca tcaggtagcc gaagtttttc
    15061 tttgtgttgg gcggattttt tcccgtctcc cagcgcatca cactgcgccc gaacggatgg
    15121 cactgcccgc tgtccggata catattcacc atctgataac gccagcgttc tttgggcagg
    15181 attggggacg gatattcatt acagacggcg ttatttttcc cgatggtttc cataatcatg
    15241 ccctgacggt gtagtttgaa cgccatgcgt tcactgacca gcagggagga ctgcaacgga
    15301 ctggactcat tactcaccca gccattgaac gggtacatac ttccctgcga accggcacac
    15361 cagaacagaa catcgagagg catattaaag gcgctggcaa tggcatctgc tgcacaggcc
    15421 ccctgtgcta tcggattggc aaagatgaca gcttccggat tgagaatggt ggtcaggctg
    15481 ctgtccgtcc aggtggggtc aatttcagaa agataagcga tatccaggtc accaccttcc
    15541 agacagccca gcgatgtgat gatgttcagc cagtacgtca gcgggtattt gtaccagtga
    15601 acgtgataga acgccccgtt cacctgcttc tcatcctttt tcgccgttcc ctgtgccgtt
    15661 ttaccaaaag ccggcaggct gaagcccagg ttaaccatgc atcccggtga ccgggtgacg
    15721 tccgtcagcg ccatcggctc ccagtaacca atggccagcc cgatacgcct gaacagcggc
    15781 ggcggtgccg gacaaatctg aatgggcatc gacgggttcg ccgtgtcggg gaccttaccc
    15841 tgactgactt tgacactgcc cagcgagagc gggaaaatac agctccagca gatatccgtg
    15901 atcgggttta caaaacgccc ctcacaggca gaatccgcag acgctgcagg gacatgaccg
    15961 gcgagaagga ataacatcag cagccacagg cttcgcttca ttttctgccc tcctctgccg
    16021 gaataaattc caccttcagg aagcgatcgc cgggaacggc gctcacccgt gccgggacct
    16081 gatcaatgcc gaggcgctgg cagagcacac cgttctggtc aaagtaaaca cggctgtcca
    16141 gagacttctg catctccggg atactgccct gcacgaggat aattttgctc tccagtgtgg
    16201 gcggcgtctg gcgtttcatc caggccacct gcgccggatc atcgccattg atgaaataca
    16261 gcgtctggtt aaaaggcaca tactgcagcg gattcatcac ttcaccctga cgggcaaaca
    16321 cccgcccttc gttatcccgg atatccgcag ccagtctgac ggacgggtca aacaaccggc
    16381 tgccgtattt ctccgtgcgt ccgataccgg gcacggcagg gggccgcaga ctgttgcgga
    16441 tcacccgctc cttaaaggca tccattttcc ggcccatctc accggactgc tccagtgccg
    16501 tcaggcgctg catgatcacc gtcagcatgt ccggctcctt taccggccac agatc
//
LOCUS       DAZPJR010000085        16395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPJR010000085 DAZPJR010000000
VERSION     DAZPJR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.086"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..578
                     /locus_tag="ACHVB7_002571"
     CDS             <1..578
                     /locus_tag="ACHVB7_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR1914525.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="ACHVB7_002572"
     CDS             581..1744
                     /locus_tag="ACHVB7_002572"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1914526.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="ACHVB7_002573"
     CDS             1828..3453
                     /locus_tag="ACHVB7_002573"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1914527.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHVB7_002574"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHVB7_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR1914528.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHVB7_002575"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHVB7_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR1914529.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHVB7_002576"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHVB7_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1914530.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHVB7_002577"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHVB7_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR1914531.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHVB7_002578"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHVB7_002578"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR1914532.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHVB7_002579"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHVB7_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHVB7_002580"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHVB7_002580"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR1914533.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9726
                     /gene="ulaC"
                     /locus_tag="ACHVB7_002581"
     CDS             9262..9726
                     /gene="ulaC"
                     /locus_tag="ACHVB7_002581"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR1914534.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9740..10390
                     /gene="ulaD"
                     /locus_tag="ACHVB7_002582"
     CDS             9740..10390
                     /gene="ulaD"
                     /locus_tag="ACHVB7_002582"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR1914535.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10400..11254
                     /gene="ulaE"
                     /locus_tag="ACHVB7_002583"
     CDS             10400..11254
                     /gene="ulaE"
                     /locus_tag="ACHVB7_002583"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR1914536.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11254..11940
                     /gene="ulaF"
                     /locus_tag="ACHVB7_002584"
     CDS             11254..11940
                     /gene="ulaF"
                     /locus_tag="ACHVB7_002584"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR1914537.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12037..12588
                     /locus_tag="ACHVB7_002585"
     CDS             12037..12588
                     /locus_tag="ACHVB7_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR1914538.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12663..12978)
                     /locus_tag="ACHVB7_002586"
                     /pseudo
     CDS             complement(12663..12978)
                     /locus_tag="ACHVB7_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13305..13700
                     /gene="rpsF"
                     /locus_tag="ACHVB7_002587"
     CDS             13305..13700
                     /gene="rpsF"
                     /locus_tag="ACHVB7_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR1914539.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13707..14021
                     /gene="priB"
                     /locus_tag="ACHVB7_002588"
     CDS             13707..14021
                     /gene="priB"
                     /locus_tag="ACHVB7_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR1914540.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14026..14253
                     /gene="rpsR"
                     /locus_tag="ACHVB7_002589"
     CDS             14026..14253
                     /gene="rpsR"
                     /locus_tag="ACHVB7_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR1914541.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14295..14744
                     /gene="rplI"
                     /locus_tag="ACHVB7_002590"
     CDS             14295..14744
                     /gene="rplI"
                     /locus_tag="ACHVB7_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR1914542.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14856..15827)
                     /locus_tag="ACHVB7_002591"
     CDS             complement(14856..15827)
                     /locus_tag="ACHVB7_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR1914543.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15880..16296)
                     /locus_tag="ACHVB7_002592"
                     /pseudo
     CDS             complement(15880..16296)
                     /locus_tag="ACHVB7_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(16313..>16395)
                     /locus_tag="ACHVB7_002593"
     CDS             complement(16313..>16395)
                     /locus_tag="ACHVB7_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1914544.1"
                     /translation="KSLSFSKSVELHDKVIGYYLNIKHYQ"
BASE COUNT         3852 a         4004 c         4353 g         4186 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tcctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgagcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9421 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9481 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9541 gaagataacg acccggtgga tatcctcatc accatggcgg cggtcgatgc caacacgcac
     9601 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
     9661 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
     9721 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
     9781 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
     9841 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
     9901 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
     9961 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10021 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10081 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10141 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10201 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10261 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10321 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10381 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10441 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10501 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10561 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10621 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    10681 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    10741 ccagctggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc gtcgtcgttt
    10801 ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtga cgctggcgat
    10861 ggagatcatg gattatccgt tgatgaactc catcagcaag gcgctgggtt acgcgcacta
    10921 tctcaacaac ccgtggttcc agctctaccc ggatatcggc aacctgtcgg cgtgggacaa
    10981 cgatgtgcag atggagttgc aggccggaat cgggcatatc gtcgcggtgc atgtgaaaga
    11041 caccaaaccg ggcgtcttca aaaacgtgcc atttggcgaa ggtgtggtgg atttcgaacg
    11101 ttgcttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg agatgtggag
    11161 cgaaacggcg gaagacccgg cggcagaagt ggtgaaagcg cgtgattggg tgaaagcgcg
    11221 catggccaaa gcaggcacgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt
    11281 gaagccaaca tggatctgcc gcgctatggg ctggtgacct ttacctgggg caacgtcagc
    11341 gctatcgacc gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc
    11401 atgaaagcag acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat
    11461 cgcccgtctt ccgacaccgc gacgtatctc gaactctacc gtcgttaccc gtcgcttggc
    11521 ggcattgtcc atacccactc cactcatgct actgcatggg cgcaggcggg gctggcgatc
    11581 ccggcgttag gcactacgca cgccgactac ttctttggcg acatcccgtg tacgcgcgga
    11641 ttaagcgaag aagaggtgca gggcgagtat gaactgaaca ccggcaaagt gattatcgaa
    11701 acgctgggtg acgccgagcc gctgcatacg ccggggattg tggtgtatca gcacggtccg
    11761 ttcgcctggg ggaaagatgc ccacgacgcg gtgcataacg cggtggtgat ggaagaagtg
    11821 gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac tgaatcacat cgacagcttc
    11881 ctcatgaata aacacttcat gcgtaagcac ggtcctaacg cctattacgg gcagaagtaa
    11941 gcagtgcgcc ccggaattat tacctcctgc gggatgtaat tggtctgatt ccggggccgc
    12001 ttcagttaaa aaatcgtata aacaacaggt gtttttatgg ctatcaatgg attgtttgtc
    12061 agaacggatc cgcaacgtcg ccgctatggc gttgcgttac ttgtgggtgt actttcagga
    12121 attatttcag catttgttaa atggggagcg gaagtcccct tgccaccgcg cacattttcc
    12181 ggtggtcgcg atgaatttaa cccgccgtat atctttctac gggattattt aggtatcgac
    12241 ccaacccaaa ctgtgtatac attctcggaa catattatca acccggtgat ggtgacgcat
    12301 atcatcttct cactggtttt tgcgataggg tattgtattg tcgcggaaat tttcccgaaa
    12361 gtgaaattat ggcaggggat attagcaggg cttattgtga cagtggcagt ccacggtata
    12421 ttttgcccgg cgctgaattt aacgccgccg ctcactcaat tgccttttga cgaatatgcc
    12481 tctgaaatcc tggggcatat tttctggttc tggataatag aaattatgcg ccgcgactta
    12541 cgtaatcgca ttactcatga gccagatccg gaagtcgcaa tccggtgaat aaccgcataa
    12601 aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg
    12661 tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg ttcccgcatc
    12721 agaattacgt ggtagtacgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt
    12781 tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa
    12841 attatgattg atataaatca cgcctaagct ttgcacatca tccatgtttc tggcctggtg
    12901 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
    12961 taaaactcga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg
    13021 atgatcttct gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg
    13081 cagttgttca aaaaaacacc atgatgaagt gtgatgaact ccaaatcagc ggtttagagg
    13141 ttaattgcga aaggggagat ttatttcggc tctgcccttg agtttagcgg ggcatacaag
    13201 tactataacg cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg
    13261 gctgacccag acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa
    13321 tcgtttttat ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca
    13381 ctgctgccat cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc
    13441 agctggctta cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag
    13501 ctccgcagga agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc
    13561 gcagcatggt tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga
    13621 aagacgagcg ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag
    13681 ctggggattc tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt
    13741 gcaggactcc ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg
    13801 agcatcgttc tgtgcaggag gaagccggtt ttcaccggca ggcgtggtgc caaatgcccg
    13861 ttattgttag cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca
    13921 taaccgttca ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt
    13981 tgcatgccga gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc
    14041 cgtcgtcgca agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat
    14101 atcgctacgc tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc
    14161 ggtacccgtg caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg
    14221 tccctgctgc cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt
    14281 gagaggataa ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg
    14341 gtgatcaggt aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag
    14401 ctgttccagc taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag
    14461 ctaaactggc tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg
    14521 aaactgttac catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta
    14581 ctcgcgacat cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc
    14641 gtctgccgaa cggcgttctg cgtaccactg gcgagcacga agtgagcttc caggttcaca
    14701 gcgaagtatt cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag
    14761 acgtaaaaac gccgaccagt ggtcggcgtt ttgcttttgt agcgagcact tgcagatatc
    14821 gataagggcc acaataagca gccctacgaa gataattact tagctgcctg aatatcagcc
    14881 agcattgggg caagggctgg gttgtcctta gcgaagtttg cgtacattgg ctgtactttc
    14941 tcgataaacg gagctttatc tacctggtag actttcacgc ccattttatc cagttttgca
    15001 aattcttgtt tctcttcctc gctccacaag tctttcatgt agctcattga ttctttagct
    15061 gcttttaata taactgcctg attttccggg ctaagtttat caaacgcagc attgctcatc
    15121 agaaccacat cgggaaccat agtgtgttcg tcaaggctga aggattttgc cacttcaccg
    15181 tgacgcatgg tggtcagcgc catcacgctg ttttctgcca tatcgaccac accctgctgt
    15241 aaggctgtat acaactcgcc gtaatccagt ggcgtcgggt taccgcccat gactttgatc
    15301 atttcaactg cgccagggct tggctggaca cggactttca tgcctttcag atcgtctggt
    15361 ttcagtactg gtttattgcc atagaaactg cgggcgcctc catcataaaa agtcagcccg
    15421 aaataacctt tgcttttcgt tgagtcgagg atttttctcc cgatatcgcc ctgcatgacc
    15481 tgataataat gatcacgatc gcggaacaaa taaggcaggc taaataattt ataggaattt
    15541 tcaaatgctt ccatttctgc cgcattggtt tttaccagtg gaatagcgcc agaacgaatc
    15601 agctccattg tttctcgttg agtacccaat gttccatttg gataaatacg aatagtaata
    15661 tcaccgttag agtactcttt gttctttttc gcaaagaact ccatcgcttt atgaacagga
    15721 tgagacttat cctgattatg actcagtttt aatgtcactg cccaggactg gcttgcggat
    15781 aacataagaa cagcaccgat aatgccagcg gatactttta ttttcataga tgttcctttt
    15841 ctaataaata tggcggcagg tgccgccagt gaatgcgcat cagtagccca gtgcttttgg
    15901 caatgccata ctgatttgcg gaatataggt caccattgcc agcaccaata ccatcacggc
    15961 atagaatggc agcataggtt tgataatttt attaatcggt aatttagata cactacaacc
    16021 gacaaataac acactgccta caggcggagt acaaataccg atacacatgt tcagtaccat
    16081 gacaatacca aagtgaatcg ggtccatgcc cagttcggtg acaacaggta aaaatatcgg
    16141 cgtaaaaatc agtaccgccg gtgtcatatc cataaaagtg ccgataatca acaggataat
    16201 attgataata agcaggatta ttattgggtt gtcagaaata gtgttaagca aatcgagaat
    16261 caggaaggga acgtcagcgt ttgacattgc ccatgacggt aatgactcca acttattgat
    16321 agtgttttat gttcagataa tacccgatga ctttgtcatg cagctccacc gattttgaga
    16381 acgacagcga cttcc
//
LOCUS       DAZPJR010000086        16392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPJR010000086 DAZPJR010000000
VERSION     DAZPJR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.087"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="ACHVB7_002594"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="ACHVB7_002594"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR1914545.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="ACHVB7_002595"
     CDS             complement(2362..2802)
                     /locus_tag="ACHVB7_002595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1914546.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHVB7_002596"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHVB7_002596"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR1914547.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="ACHVB7_002597"
     CDS             3520..4218
                     /locus_tag="ACHVB7_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR1914548.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="ACHVB7_002598"
     CDS             4447..5655
                     /locus_tag="ACHVB7_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1914549.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="ACHVB7_002599"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="ACHVB7_002599"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR1914550.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="ACHVB7_002600"
     tRNA            7762..7838
                     /locus_tag="ACHVB7_002600"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
     gene            7873..8006
                     /locus_tag="ACHVB7_002601"
     ncRNA           7873..8006
                     /ncRNA_class="other"
                     /locus_tag="ACHVB7_002601"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8751..10358
                     /gene="dppA"
                     /locus_tag="ACHVB7_002602"
     CDS             8751..10358
                     /gene="dppA"
                     /locus_tag="ACHVB7_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR1914551.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10666..11685
                     /gene="dppB"
                     /locus_tag="ACHVB7_002603"
     CDS             10666..11685
                     /gene="dppB"
                     /locus_tag="ACHVB7_002603"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR1914552.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11695..12597
                     /gene="dppC"
                     /locus_tag="ACHVB7_002604"
     CDS             11695..12597
                     /gene="dppC"
                     /locus_tag="ACHVB7_002604"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR1914553.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12608..13591
                     /gene="dppD"
                     /locus_tag="ACHVB7_002605"
     CDS             12608..13591
                     /gene="dppD"
                     /locus_tag="ACHVB7_002605"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR1914554.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13588..14592
                     /gene="dppF"
                     /locus_tag="ACHVB7_002606"
     CDS             13588..14592
                     /gene="dppF"
                     /locus_tag="ACHVB7_002606"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR1914555.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14622..15893)
                     /locus_tag="ACHVB7_002607"
     CDS             complement(14622..15893)
                     /locus_tag="ACHVB7_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1914556.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3899 a         4087 c         4407 g         3999 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcaacg
     7861 agcaggcttt tttgcatttg tagctcatga ggatgagaac ctccgggggc agggaggttc
     7921 gactcgagcg aagcgagaga acgttgcgcc agcaacggcc cgcagggcga gccacgaagt
     7981 ggcgagtaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggcttttt
     8041 tgcatttgta gctcatgagg atgagaacct ccgggggcag ggaggttcga ctcgacgtag
     8101 gcctgataag acgcgccagc gtcgcatcag gcaacggcac cgcatttatt gtcggatgcg
     8161 gcgcgaccgc cttattcgac caacacacca cacattttcc tgccaacttt gcgccccata
     8221 tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa
     8281 atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc
     8341 atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcatta aatgcataaa
     8401 acttaatcac caaatgttgc taaatattaa gctgattgtt ctggttatgt tggagtagca
     8461 caaatttctc tgctgcaaat agctgtttga agttttttta atctttgttt gtggattctc
     8521 accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt gacagattgt
     8581 aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta attagtacgg
     8641 catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc aggcagcgac
     8701 aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata atgcgtattt
     8761 ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg accgtcgcag
     8821 caagtgttca ggctaaaact ctggtttatt gctcagaagg atctccggaa gggtttaacc
     8881 cgcagctgtt tacctccggc accacctatg acgcctcttc cgtaccgctt tataaccgcc
     8941 tggttgaatt taaaatcggc accactgaag tgatcccggg cctcgctgaa aagtgggaag
     9001 tcagcgaaga cggtaaaacc tataccttcc atctgcgtaa aggtgtgaag tggcacgaca
     9061 ataaagaatt caaaccgacg cgtgaactga acgccgatga cgtggtgttc tcgttcgacc
     9121 gtcagaaaaa cgcgcaaaac ccgtaccata aagtctctgg cggcagctac gaatacttcg
     9181 aaggcatggg cttgccggag ctgatcagtg aagtgaaaaa ggtggacgac aacacagttc
     9241 agttcgtact gactcgcccg gaagcgccgt tcctcgctga tctggcaatg gacttcgcct
     9301 ctattctgtc aaaagaatat gctgatgcga tgatgaaagc cggtacaccg gaaaaactgg
     9361 acctcaaccc aatcggaacc ggtccgttcc agttacagca gtaccaaaaa gattcccgta
     9421 tccgctacaa agcgtttgat ggctactggg gcaccaaacc gcagatcgat acgctggttt
     9481 tctctattac ccctgatgct tccgtgcgtt acgcgaaatt gcagaagaat gaatgccagg
     9541 tgatgccgta cccgaacccg gcagacatcg ctcgcatgaa gcaggataaa tccatcaacc
     9601 tgatggaaat gccggggctg aacgtcggct acctctccta taacgtgcag aaaaaaccgc
     9661 tggatgacgt gaaagttcgc caggcactga cctacgcggt gaacaaagac gcgatcatca
     9721 aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct gatcccgcca accatgtggg
     9781 gctataacga cgacgttcag gattacacct acgatcctga aaaagcgaaa gccttgctga
     9841 aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg gtacaacgtc
     9901 cgtataaccc gaacgctcgc cgcatggcgg agatgattca ggcagactgg gcgaaagtgg
     9961 gcgtgcaggc caaaatcgtc acctacgaat ggggtgagta cctcaagcgt gcgaaagatg
    10021 gcgaacatca gacggtaatg atgggctgga ctggcgataa cggggacccg gataacttct
    10081 tcgccaccct gttcagctgc gccgcctctg aacaaggttc taactactca aaatggtgct
    10141 acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccat aacaaacgcg
    10201 ttgaactgta caagcaagcg caggtggtga tgcacgatca gactccggca ctgatcatcg
    10261 ctcactccac cgtgtttgaa ccggtacgca aagaagtcaa aggctatgtg gttgatccat
    10321 taggcaaaca tcacttcgaa aacgtctcta tcgaataatt aaaagccata caagactgat
    10381 ggcaaaggca aaactgcctg atgtgctccg cttatcaggc ctacgaaaat tctgcaatgt
    10441 attgaatttg cacgattttg taggccggat aaggcgttaa cgccgcatcc ggcataaaca
    10501 aagcgcactt tgtcaacaat ctgtataccc ggtggcgatg tgcctctgcg tgcagcgcca
    10561 tccggcagca atacttcatt ccctgtccga taagggcggg aattgatttg tgagcaatac
    10621 agacacgcag ttccaggctg cgggtcacta tagagaatcc gggttatgtt gcagtttatt
    10681 ctccgacgtt tgggactcgt catccccacg tttatcggta ttacccttct cacatttgcc
    10741 tttgtccaca tgatcccggg cgatccggtg atgatcatgg caggcgaacg cgggatctcc
    10801 cctgagcgtc acgcgcagct gctggctgaa ctcggcttag ataaaccgat gtggcagcag
    10861 tatctccatt acatttgggg cgtaatgcac ggcgatctag gcatttcaat gaaaagccga
    10921 attccagtat gggaagagtt cgtgccgcgc tttcaggcca cgctggaact tggcgtctgc
    10981 gcgatgattt ttgctacggc agtcggtatt ccggttggtg tgctggctgc ggttaaacgc
    11041 ggttccattt tcgatcacac tgcagttggc ctggcgctga ccggctactc gatgccgatc
    11101 ttctggtggg gcatgatgct gatcatgctg gtttcggtgc actggaacct gacgcccgtc
    11161 tccggtcgcg tgagcgatat ggtgttcctc gatgactcca atccgttaac cggttttatg
    11221 ctgatcgaca ccgccatctg gggtgaagat ggcaacttta tcgacgccgt tgcccatatg
    11281 atcttaccgg ctattgtgct gggtactatc ccgctggcgg ttatcgttcg tatgacgcgc
    11341 tcttcgatgc tggaagtgct gggtgaagat tacatccgca ccgcgcgcgc caaagggttg
    11401 acccgcatgc gggtgattat cgtccatgcg ctgcgtaacg cgatgctgcc ggtagtgacc
    11461 gttatcggcc tgcaggtggg aacattgctg gcgggggcga ttctgaccga aaccatcttc
    11521 tcgtggcccg gtctgggacg ctggttgatt gacgcactgc aacgccgcga ctatccggta
    11581 gtgcagggcg gcgtattgct ggtggcgacg atgattatcc tcgtcaacct gctggtcgat
    11641 ctgctgtacg gcgtggtgaa cccgcgtatt cgtcataaga agtaagggga catcatgtca
    11701 caggttactg aaaataaagt gattagcgca ccggtgccga tgaccccgtt acaggagttc
    11761 tggcactatt ttaaacgcaa caaaggcgcg gttgtcgggc tggtttacat cgtcatcgtg
    11821 ctgtttatca cgatctttgc caactggatt gcgccttata acccggcgga tcagttccgc
    11881 gatgcgctgc tcgccccgcc agcctggcag gaaggcggca gcatggcgca cttgctgggc
    11941 accgatgacg taggccgtga tgtgctgtcg cggctgatgt acggcgcgcg cttgtcgctg
    12001 ctggttggct gtctggtggt ggtgttatcg ctgattatgg gcgttattct cggcctgatc
    12061 gccggttact tcggcggcct ggtcgataac atcatcatgc gcgtggtcga tatcatgctg
    12121 gcgctgccaa gtctgctgtt ggcactggtg ctggtggcga ttttcggccc gtcgattggt
    12181 aacgccgcgc tggcactgac cttcgttgcc ttgccgcact atgtgcgctt aacccgcgcc
    12241 gccgtgctgg tggaggtgaa tcgcgattac gttaccgcgt ctcgcgtggc aggtgccggg
    12301 gcgatgcgtc agatgtttat taacatcttc ccgaactgcc tggcgccgct gattgttcag
    12361 gcgtcgctcg gtttctctaa cgccattctc gatatggccg cccttggctt cctcggcatg
    12421 ggagcgcagc cgccaacacc agagtggggc accatgctct ccgacgtgtt gcagttcgcg
    12481 caaagcgcct ggtgggtcgt gaccttcccg ggtctggcga tcctgctgac ggtgctggca
    12541 tttaacctga tgggtgacgg tctgcgtgac gcgctcgatc ccaaactgaa gcagtaagag
    12601 gttcgagatg gcgttattaa atgtagataa attatcggtg catttcggcg acgaaagcgc
    12661 accgttccgc gccgtagacc gcatcagcta cagcgtaaaa cagggcgaag tggtcgggat
    12721 tgtgggtgag tctggctccg gtaagtcggt cagttcactg gcgattatgg ggctgattga
    12781 ttatccgggc cgcgtaatgg cggaaaagct ggtgtttaac ggtcaggatt tgcagcgtat
    12841 atcagaaaaa gagcgccgca acctggtggg tgccgaagtg gcgatgatct tccaggaccc
    12901 gatgaccagc cttaacccgt gctacaccgt gggtttccag atcatggaag cgattaaggt
    12961 gcatcagggc ggtaataaga gcactcgccg tcagcgggcg attgacctgc tgaatcaggt
    13021 cggtattccc gatccggcat cgcgtctgga tgtttacccg catcagcttt ccggcggcat
    13081 gagccagcgc gtgatgatcg ccatggcgat tgcctgtcgg ccaaaactgc tgattgccga
    13141 tgagccgacc actgcgctgg acgtgaccat tcaggcgcaa atcatcgaac tgttgctgga
    13201 gctacagcag aaagagaaca tggcgctggt gttaattacc catgatctgg cgctggtggc
    13261 ggaagctgcg cataaaatca ttgtgatgta tgcaggccag gtggtggaaa cgggcgatgc
    13321 gcacgccatc ttccatgcgc cgcgtcaccc gtatactcag gcattgctgc gtgcgctgcc
    13381 agaatttgct caggacaaag aacgtctggc gtcgttgcca ggtgtcgttc ccggcaagta
    13441 cgaccgcccg aacggctgcc tgcttaaccc gcgctgcccc tatgccactg acagatgtcg
    13501 cgctgaagaa ccggcgctga atatgctcgc tgacgggcgc cagtccaaat gccattaccc
    13561 acttgatgat gccgggaggc cgacactatg agtacgcaag aggccaccct gcaacaaccg
    13621 ctgttgcagg ctatcgacct gaaaaaacat tatccggtga agaaaggcat gttcgcgccg
    13681 gaacgtctgg ttaaagcgct ggatggcgtt tcgtttaacc ttgaacgtgg caaaacgctg
    13741 gcagtagtgg gcgaatctgg ctgcggtaaa tcgaccctcg gtcggttgct gacgatgatt
    13801 gaaacgccca ccggtggcga gctgtattac caggggcagg atctgcttaa gcacgatccg
    13861 caggcgcaga agctgcgtcg gcagaaaatc cagatcgtct tccagaaccc ttacggttcg
    13921 ctgaatccgc gtaaaaaagt cgggcaaatt cttgaagagc cgctgctgat caacaccagc
    13981 ttaagcaaag aacagcgtcg ggaaaaagcc ctgtcgatga tggcgaaagt cggcctgaaa
    14041 accgagcact atgaccgcta tccgcatatg ttctccggcg gtcagcgtca gcgtatcgcc
    14101 atcgcccgtg gtctgatgct cgacccggat gtggtgattg ccgatgaacc ggtttccgcg
    14161 ctggatgttt cagtgcgcgc gcaggtgctg aatctgatga tggatttgca gcaggagttg
    14221 gggctgtctt atgtctttat ctcccacgac ctgtcggtgg tggagcacat tgctgatgaa
    14281 gtgatggtga tgtacctggg ccgctgcgtg gagaagggaa cgaaagacca aatcttcaat
    14341 aacccgcgcc atccgtacac tcaggcgcta ctttccgcga cgccgcgcct gaacccggac
    14401 gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa gcccactgaa tccaccgccg
    14461 ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc cctgcaccca gttgcagccg
    14521 cagctaaaag actacggcgg tcaactggta gcttgttttg ctgttgatca ggatgaaaat
    14581 ccgcagcgtt aactcacaaa accggggcca tgcgctccgg tttactcaat cagcttcaac
    14641 agtggcgaca agcatagcaa aatgccgtac agcagaatca gccaggcttt aaatccgcga
    14701 agtttttcca gttgtgcgac tttatagatc aggaaaaacg gaatgaggca cgacacaata
    14761 ccatataacg ggcttcccaa ctgaaagaac accagcaccg atacacgaaa cgaaacccaa
    14821 atcgtcaacg taatgacgat aaaagcgcag atcgccagag tcagcacgcg tgagttaatt
    14881 ttcttggtat caataattcg gcttaacaga ttgagaataa tgcctttaat ggcctcgtgg
    14941 aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa agatattaag caccgtagag
    15001 gtgatatgaa tgatatgccc agggatcacc tgcgcggcca gcgccagtgc tgagatattt
    15061 tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga aggtaaacga aaaggcaaaa
    15121 aacaggatca ccgcgatgag cgtgatataa ctaattcggt gggtacgcag cgcgagccgg
    15181 gttgccagta ctttatccgc ttcccgttta cgataggcaa tattcattgg gttaagtacc
    15241 tgaataaata ctgcagaaaa gaagcaaaat ggaatggtaa gcaagacatc gcggaaaaag
    15301 acggaggctt gcgggaaggc ggttatattg gcgaaattcc agtgcgggat catcgcaaaa
    15361 ccgaacacga caataatccc tactttgacc accaccattg gcccggaaat cttaaacagt
    15421 aatcgttcac caccagacgc aatcgctacc agtacggcga aaatagcgac tttataaagt
    15481 agagattgtg aaagatcggc atcggttaaa ccgaaggttt tcaggtacga ggcgctgtcg
    15541 aaaaccacgg agagagagta gataaaaatc ccgtggataa tcatcaaaaa gtagataacc
    15601 ccgaggaaaa ttccccagtt cttccccagg taatgactga taatatcggt gtagtcatta
    15661 caggaatcgc tttcagaaag ggtttttaaa taaatgtcct gcactaccca ggtggcagga
    15721 taagcaatga tcgctgcggt aataaatacc caaattccct tcaagccaat ttgtactggc
    15781 atcagcacgg ttccggcacc aatcgccatg cctatgcata ataaaaccca gccgaagtcg
    15841 tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg tgttgtgctg cataaagtcc
    15901 tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc agtttctctt gcgcaacagg
    15961 tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac agattcactg gggaggtcgc
    16021 gaggaggcgc aattctacaa aattgcgcat ttcactacca gtctggagtc agcccggata
    16081 tgaactgcaa ctattgcaaa taaagaaatt aagctggaag gcatcgcgca atgaggaaga
    16141 ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc ggcaagccga aagggttccg
    16201 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
    16261 cccccgcacg ttgcaggtat gtacctgaca acataacggg ggctaatctt gaacctagac
    16321 cactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
    16381 gagctgggca tg
//
LOCUS       DAZPJR010000087        16272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPJR010000087 DAZPJR010000000
VERSION     DAZPJR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.088"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(103..1305)
                     /gene="ubiI"
                     /locus_tag="ACHVB7_002608"
                     /gene_synonym="visC"
     CDS             complement(103..1305)
                     /gene="ubiI"
                     /locus_tag="ACHVB7_002608"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR1914557.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1328..2506)
                     /gene="ubiH"
                     /locus_tag="ACHVB7_002609"
                     /gene_synonym="visB"
     CDS             complement(1328..2506)
                     /gene="ubiH"
                     /locus_tag="ACHVB7_002609"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR1914558.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2503..3828)
                     /gene="pepP"
                     /locus_tag="ACHVB7_002610"
     CDS             complement(2503..3828)
                     /gene="pepP"
                     /locus_tag="ACHVB7_002610"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR1914559.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3854..4432)
                     /gene="ygfB"
                     /locus_tag="ACHVB7_002611"
     CDS             complement(3854..4432)
                     /gene="ygfB"
                     /locus_tag="ACHVB7_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR1914560.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4600..4929
                     /gene="zapA"
                     /locus_tag="ACHVB7_002612"
     CDS             4600..4929
                     /gene="zapA"
                     /locus_tag="ACHVB7_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR1914561.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4971..5154
                     /gene="ssrS"
                     /locus_tag="ACHVB7_002613"
     ncRNA           4971..5154
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHVB7_002613"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5175..5777
                     /locus_tag="ACHVB7_002614"
     CDS             5175..5777
                     /locus_tag="ACHVB7_002614"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR1914562.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5878..5937)
                     /locus_tag="ACHVB7_002615"
     CDS             complement(5878..5937)
                     /locus_tag="ACHVB7_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1914563.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6269..6328)
                     /gene="ibsC"
                     /locus_tag="ACHVB7_002616"
     CDS             complement(6269..6328)
                     /gene="ibsC"
                     /locus_tag="ACHVB7_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR1914564.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6557..7789)
                     /gene="serA"
                     /locus_tag="ACHVB7_002617"
     CDS             complement(6557..7789)
                     /gene="serA"
                     /locus_tag="ACHVB7_002617"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR1914565.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8045..8704)
                     /gene="rpiA"
                     /locus_tag="ACHVB7_002618"
     CDS             complement(8045..8704)
                     /gene="rpiA"
                     /locus_tag="ACHVB7_002618"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR1914566.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            9134..10027
                     /gene="argP"
                     /locus_tag="ACHVB7_002619"
     CDS             9134..10027
                     /gene="argP"
                     /locus_tag="ACHVB7_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR1914567.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10230..12374
                     /gene="scpA"
                     /locus_tag="ACHVB7_002620"
     CDS             10230..12374
                     /gene="scpA"
                     /locus_tag="ACHVB7_002620"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR1914568.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12367..13362
                     /gene="meaB"
                     /locus_tag="ACHVB7_002621"
     CDS             12367..13362
                     /gene="meaB"
                     /locus_tag="ACHVB7_002621"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR1914569.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13373..14158
                     /gene="scpB"
                     /locus_tag="ACHVB7_002622"
     CDS             13373..14158
                     /gene="scpB"
                     /locus_tag="ACHVB7_002622"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR1914570.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14182..15660
                     /gene="scpC"
                     /locus_tag="ACHVB7_002623"
     CDS             14182..15660
                     /gene="scpC"
                     /locus_tag="ACHVB7_002623"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR1914571.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15657..>16272)
                     /locus_tag="ACHVB7_002624"
     CDS             complement(15657..>16272)
                     /locus_tag="ACHVB7_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1914572.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         3951 a         4383 c         4144 g         3794 t
ORIGIN      
        1 cggaggtgaa attagtttat ttcaaatgag gaaaatctcc cggcgaaaaa accgggagat
       61 gaaagtgtga tgggtatcaa ataaacagca gaagagaaat ttttaacgca gccattcagg
      121 caaatcgttt aatcccattg cctggcggat aagttgtggt ttaacgccag gaagcgtgtc
      181 ggccagtttc aaaccaatat cacgcagcag ttttttcgcc ggattggtac cggaaaacag
      241 atcgcggaat ccctgcatac cagccagcat cagcgccgca ctgtgcttgc ggctacgctc
      301 ataccggcgc aggtaaatgt actgcccgat gtctttcccc tgacgatgca accgtttcag
      361 ttcagcaatc agctctgcag catccataaa gccaagattt actccctgcc ccgccagcgg
      421 gtgaatggta tgcgcggcgt cgcccaccag caccagacgg tgcgcggcaa actggcgcgc
      481 ataacgcccc gtcagtggga acacctgacg cgcgctctca accttgcata agcccaggcg
      541 attatcaaaa gcgatattta acgcgcgatt aaattcatct tcacttgcct gctgcatccg
      601 ctgcgcttcc tctggcgaca gtgaccagac aattgagcaa agatgcggat cgctaagcgg
      661 taaaaaggcc agaatgcctt cgccatggaa aacctgccgc gccaccgcat catgcggttc
      721 ttccgtgcga atggtcgcta ccagcgcgtg atgctgataa tcccagaaag tcagcggaat
      781 atctgctttg ttgcgcaacc aggaattagc accgtccgcg ccaatcacca gacgcgccgt
      841 taacatgctg ccatctttca gcgtcaggaa agtttcattt tctccccagg cgacctgctg
      901 taattctgcg ggggccaaca gagtgatatc tgacgactgc tgcgctttgt tccacagcgc
      961 gtagtgaatc actgagtttt caacgatatg cccaagatgg ctatagccca tgctttgatc
     1021 gtcaaacgaa atgtgaccaa aactgtcttt gtcccacact tccataccgt gataacaact
     1081 ggccctacga gagagaatgt cctgccagac gccaagacgg gtgagtaatt tttcgctggc
     1141 ggcattgata gccgaaacgc gcagttgtgg tggtgcatcc gccgccagag gttccggtac
     1201 gcgctgctcc agtacggcaa cgcgtaagcc gctcccctgt aagccgcagg caaccgccag
     1261 ccccaccatg ccgccaccaa caatggctac atcaacactt tgcattgttt attccttaaa
     1321 accgccttca acgcgccacc caaccgaggg tgcgctgcgc cagcacatcg cgtgccgggg
     1381 tgaataattc catcgtcatc agtccgatgt tgcgcccgac aaccagcggt gcccaacggt
     1441 tggcaaaaag atgtaccagg ctgtccgtga cgccgatggt tgcttcgcga tcgctctgtc
     1501 gacgctgctg atagcggcac aatatgccgt aatcaccgat gtcttctccg cgctcatgcg
     1561 cctgagtcag ggtttccgca aggctcatga catctcgcat accgaggtta aacccttgcc
     1621 cggcaatcgg gtgcagagtt tgcgccgcat tgcccaccag cacggtacga tgggtaatag
     1681 ctctggcggc gcgggttaac gccagcggat aagcactgcg tttaccggcg tgggtaattt
     1741 ttccaagtcg ccagccaaag gccgactgga gttcacggca aaacttctcg tcgctccacg
     1801 acagcacctc ttcgcgccgt tccagtggat gacaccagac cagcgaacag cgtccgtcag
     1861 acatcggcaa catcgccagc gggccatgtt gcgtgaagcg ttcaaaagcg cggccttcgt
     1921 gcgcaacgga agtcgcaacg ttggcaatca cggccagttg ttcgtaaggc tcctgctgcc
     1981 agtcaacgcc gcacgcggtg gctaacgctg aatgggtgcc atcagctgct accagcacgc
     2041 ggcccgtcag cgtctcgcca ctctccagcg tcacttcaac gtgactctga gtacgggcaa
     2101 cgttagccac gcgatcaggg caatgcagcg ttacgccagg tgctttacgc agcaatgcaa
     2161 acagccgttg cccgacattg tgcaattcga caacctgtcc cagcgccgcc agttggtaat
     2221 cttctgcggc gagggtgaca aatccagcgt gaccacgatc gctgacatgc acggtagtga
     2281 tggcggttgc gtaatccgcc agagattgcc agacgccgat gcgcgccagt tgctgacagg
     2341 tccccgcagc cagcgcaata gctcgtccat caaagcccgg atgagcatgt gactctggcg
     2401 cggtcgcttc aatcaaatgt accggcagcg ccccgtgact taaccgggaa atagccagcg
     2461 ccagcgtcgc gcccgccatg ccgccaccga cgatgattac gctcattgct ttctcgcagc
     2521 tgccatcaac gcttcgattt cttccggctt tttcaccacg ctggcggtga ggttttcgtt
     2581 gccggtttcg gtaatcacaa tgtcgtcttc aatacgaatg ccgataccgc gatattgttc
     2641 tggcacatcc gcatccggcg cgatatacag accaggctct acggtcagta ccatccccgg
     2701 ttccagaatg cgcgagcgat cctgaccata aacgccaacg tcatggacat ccagtcctaa
     2761 ccagtggcta aggccatgca taaagaaagg acgatgggcg ttctgagcga tcagttcatc
     2821 aacatcacct ttcaggatgc cgagttttac caggccgcta accatgatgc gcaccacttc
     2881 accagtgact tccagaatgg aagttcccgg acgatacagg cgcaggctgg tttcgagaga
     2941 ctccagcaca atgtcgtaga tttcacgctg ggcctgggtg aatttgccgt tgaccgggaa
     3001 ggtgcgggta atatcgcccg cgtaaccttt gtattcacaa cccgcgtcaa tcaacaccag
     3061 gtcaccgtcg cgcagttcac tctcgttttc ggtgtagtgc agaatgcagc cgttttcacc
     3121 gctgccgaca atggtgttat aggacggata gcgcgcaccg tggcggttaa attcgtggtg
     3181 aatttcgcct tccagatggt actcgaacat tcccggacgg catttttcca tcgcccgtgt
     3241 atgtgccatg gcggtgattt ctcccgcgcg gcggagtacg gcaatctctt ctggcgattt
     3301 gaacaggcgc atttcatgaa caacaggacg ccagtcgatc atcgttgccg gtgcggtgag
     3361 attttgccgc gaacctttac gcagtttttc cagcgcactg ttcatgattt catcagcata
     3421 tgcatattcg ccctgggcat ggtaaaccac atccaggccg ttaagtagtt gataaagttg
     3481 ctgattgatt tcgctgaatg ccagtgcgcg gtcaacgccc agtttctctg gcgcggcatc
     3541 ctggcctaag cgacggccaa accagatctc cgccgtcagg tcgcgaacgc ggttaaacag
     3601 aacgctgtgg ttatgagtgt catcgctttt aatcagcacc agcaccgctt ccaattcgtt
     3661 aaagccggtg aagtaccaga agtcactgtt ctgacgatag gggtattcgc tgtcggcgct
     3721 acgtgttact tctggtgcag caaaaatcag cgcggcgctg ccgggttgca tttgctccac
     3781 cagggcctga cggcgacgct gaaactcttg ccgggatatc tcactcataa cactctcctt
     3841 acgttttttg tttttagtgt agagtcggtt tttgtacttc tggcgcggtc ggttgcggat
     3901 gagtaaaggt gtcgtggcat aacagcgcgg caacacgaac gtattcgatg atctcttcaa
     3961 gcgacatttc aagctcttcc tgatcttcgt cttcgtcgta acccagttgc gcaatgttac
     4021 gcagatcgtc gatagcttca ccggtttcgc cggtcacttt atccagcttc ggttgcgtaa
     4081 cgccaagacc aagcaggaag tgattgaccc aacctgccaa tgcatcagcc cgatcgaaaa
     4141 cgctgacatc atcgccatca ggcagataaa gctgaaaaag gaagccgtca tcctgcaggg
     4201 catcgctggt ggcagagtgc attttacgca gtgcctgtgc cagctcatga ccgaaagcca
     4261 tgccttcgtt cgtcaggtcg tgaagtagcg gcagccatga gctgtcatcg ttaccgccac
     4321 atatcatccc actgattaaa ccatgcatct cggcaggggt cagccccgtc ccttgttggt
     4381 tcagatactg gttcatttcg ttgtaaccag gcatttcgtt ctgtatagac ataagcattc
     4441 gtcgtcaaag ggaggaatat tcatgatatg ctaccacttt gggccctggt ggaccagaaa
     4501 agggcttgtc tcttctcatc agggtagcta tagtgtcgcc ccttcgcaga ccatgggtct
     4561 aaagacgaag gcagcgcagt caatcagcag gaaggtggca tgtctgcaca acccgtcgat
     4621 atccaaattt ttggccgttc actgcgtgtg aactgcccgc ctgaccaaag ggatgcgttg
     4681 aatcaggcag cggacgatct gaaccaacgg ttgcaagatc tgaaagaacg cactagagtc
     4741 acaaatactg aacagttggt cttcattgcc gcattgaata tcagctatga gttagcgcaa
     4801 gaaaaagcga agactcgtga ctacgcggca agtatggaac agcgtattcg gatgctgcag
     4861 cagaccatag aacaagcgtt acttgaacaa ggtcgcatca ccgaaaaaac taaccaaaac
     4921 tttgaatgac acttttcggt ttactgtggt agagtaaccg tgaagacaaa atttctctga
     4981 gatgttcgca agcgggccag tcccctgagc cgatatttca taccacaaga atgtggcgct
     5041 ccgcggttgg tgagcatgct cggtccgtcc gagaagcctt aaaactgcga cgacacattc
     5101 accttgaacc aagggttcaa gggttacagc ctgcggcggc atctcggaga ttcccttctt
     5161 atctggcacc agccatgacg caactaccag aactcccact gacattatcc cgacaagaaa
     5221 tccgcaaaat gattcggcaa cgtcgtcgtg cgttaacgcc ggaacaacag caggaaatgg
     5281 gtcaacaagc cgctacccgg atgatgactt atcccccggt ggtgatggcg catacggtcg
     5341 ctgtattcct ctcttttgat ggtgaactcg acacccagcc gctaatagaa caactctggc
     5401 gcgccggtaa gcgcgtatat cttccagttt tgcatccctt tagtgccggt aatttgctgt
     5461 tcctgaatta ccatccgcaa agcgaactgg tgatgaacag gttgaagatc catgagccaa
     5521 aactggatgt gcgtgacgtg ctaccccttt cccgattaga cgtgctgatc acaccgctgg
     5581 tcgcctttga tgagtacggt cagcgcctgg gaatgggcgg tggtttttat gatcggacct
     5641 tacaaaactg gcagcactat aaaacgcaac cggtgggtta tgcgcatgat tgtcagttgg
     5701 tggaaaaact ccccgttgaa gagtgggata tccctcttcc tgcggtggtt acaccgtcga
     5761 aagtctggga gtggtaaggg cgatacacct gcatcgccct gattgacatc gttgattctt
     5821 taacctaatt tagtgagtaa gggtaaggga ggattgctcc tcccctgtga ctgactgtta
     5881 ataagctggg aaacttatga gtaacagtac aatcagtatg atggcaagtc gcatcataac
     5941 ccttctcctt caagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgccca
     6001 gctatcgtaa gctctcttta aataaccaca acgcattgat ctacctttaa atcttttctt
     6061 cgagcgtgct cgaaaaaccc atgcaaaaca gagataattt ttgggaaaaa ggatctatta
     6121 cggcagtaaa cgtgggcgat gaagtggcta tcaaaaagtt cggttcaggg cgatgcaccc
     6181 cgcatcgccc tgattgacat cgttgattct ttgacctaat ttagtgagta agggtaagag
     6241 aggatgactc ctcccctgag actgactgtt aataagctcc gaaacttatc agtaacagta
     6301 caatcagtat gatgacaagt cgcatcataa cccttctcct ttaagccctc gcttcggtga
     6361 gggctttacc gttacagccc catgctgtcc cgccatcgta aatccccatt aaataaacac
     6421 aacgcattga tctgagtttg atttattttc tggagcagac tcgcaaagta gaatgcgcaa
     6481 cgcggcaacg gtgtggagaa gggataaaaa aacgggcaag tcagtgacct gcccgttaat
     6541 tttcagagaa ggggaattag tacagcagac gggcgcgaat ggtacccgga atagctttca
     6601 ttgcctgcag cgctttttcg gcaacgtctt cgtcagcttc aatatcaata actacataac
     6661 ccatctgggc ggaagtttgt agatattgcg cggcgatgtt gacgccctgc tcggcgaaga
     6721 ttttgttcag cgcagttagc acgcccggac ggttttcgtg gatgtgcatc agacgacgac
     6781 caccatgcag tggcagcgag acttccggga agttcaccgc agagagcgtt gagccattgt
     6841 cagaatactt gatcaattta cccgcaactt ccaggccgat attctcctgc gcttcctgag
     6901 tcgaaccgcc aatgtgtggc gtcagaagga cgttgtcgaa ttcacacagt ggagaggtaa
     6961 atggatcgct attggtcgcc ggttccgtcg ggaatacgtc gattgccgcc cccgccagat
     7021 gtttgctcgc cagcgcatca cacagcgccg gaatatccac cacagtaccg cgcgaggcat
     7081 taatcagcag cgagccgggc ttcattagcg aaatctcttt cgcgcccatc atatttttgg
     7141 tggacggatt ctctggtaca tgcagactca ccacatcgct catattcagc aggtcagaaa
     7201 gatgctgtac ctgagtggcg ttgcccagcg gcagtttgtt ttcaatatca taaaagtaaa
     7261 catacattcc cagcgattca gccagaatgc ccaattgcgt accaatatga ccgtagccga
     7321 tgatacccag ctttttgccg cgcgcttcaa aagaacccgc cgccagtttg ttccacacgc
     7381 cacggtgcgc tttagcattg gcttccggca cgccgcgcaa tagcagcagc agttcgccaa
     7441 tcaccagctc cgcaacagag cgcgtatttg agaacggtgc gttaaatacc gggatcccgc
     7501 gctttgccgc cgcatccaga tcaacctggt ttgttccgat acagaaacag ccaatagcga
     7561 ccagtttttc tgcggcgttg atcacgtctt cagtcagatg ggtacgggat cgcaggccga
     7621 tgaagtgggc atcgcggatg gattctttta attgttcatc atccagcgcg cctttgtgaa
     7681 attcgatgtt ggtgtaacca gctgcacgaa ggctttccag cgccttttgg tgcacgcctt
     7741 ctaccagcag aaacttaatc ttgtctttct ccagcgatac ctttgccatt tacccaatcc
     7801 tgtcttttga aatgttgtgt gcggatttgc atccgccttt caacatatca aaaaataata
     7861 ttgcggcaat atgaacgttt gcgtcgcgat gttgaggaaa tatcacgcaa aggtatttct
     7921 tggttgaata tgctggttta gaggatttat ttagagcaat cgacaatagc caagtaaaaa
     7981 cgtgacacat gtcaccaaat ttaatgaaga gaattttttt aacgggggag gttcccccgt
     8041 cagatcattt cacaatggtt ttgacaccgt caggtgtgcc aatcagcgca acgtccgcgc
     8101 cacggttagc aaacaagcca acagtcacca cgccaggaat cgcatttatg gcgttttcca
     8161 tcgctatcgg gtcaaggatt tccatgccgt ggacgtcgag gatcacgttg ccgttatcgg
     8221 tcaccacgcc ctgacggtat tccggacgac cgcccagttt caccagctgg cgcgccactg
     8281 cactacgtgc catcgggata acttctactg gcagcgggaa tttgcccaga atatcaacct
     8341 gcttggaagc gtcagcaata cagataaatt tttccgcaac cgaagcaatg attttttcac
     8401 gggtcagcgc cgcgccgccg cctttgatca tttgcatgtg gccgttgatt tcatctgcgc
     8461 catcaacgta gatgccaagg ctgtcgactt cgttgagatc aaaaacgtga atgccgaggc
     8521 ttttcagttt ttcagtggaa gcatctgaac tggaaacggc cccttcaatc tggcctttca
     8581 ttgtaccgag cgcgtcaata aagtgtgcgg cggtggaacc tgtacctaca ccaacaatgg
     8641 tgccgggctg aacatactga agtgccgccc atcctactgc ttttttcaat tcatcctgcg
     8701 tcatgatcgt ttcgcctgtg gtatgaaatt tcacacgcat tatatacaaa aaaagcgatt
     8761 cagaccccgt tggcaagccg cgtggttaac tcatccataa aatatcgcgc aatggcgggc
     8821 atcccctttc gccccgcaaa taaagcatac aacggtctgg gtatgccgct ccacggtgcg
     8881 aacaggcgca ccagttcccc ccgttcgcaa gcccctgttt acaggcaaat tgaggcaata
     8941 acgccacccc attcaacaca accagggcac gaactcgctg agcgaaatgc attagcgcaa
     9001 atccctgatc ttttaagctg cgcgtttaac cacggataca aatgttaccc gccgacggct
     9061 tcggtatatg caacctgaca caaaattgtg tcatagtgca ggaaaaagca tttaccagga
     9121 gcagacaaca gcaatgaaac gcccggacta cagaacatta caggcactgg atgcggtgat
     9181 acgtgaacga ggatttgagc gcgcggcaca aaagctgtgc attacacaat cagccgtctc
     9241 acagcgcatt aagcaactgg aaaatatgtt cgggcagccg ctgttggtgc gtaccgtacc
     9301 gccgcgcccg acggaacaag ggcaaaaact gctggcactg ctgcgccagg tggagttgct
     9361 ggaagaagag tggctgggcg atgaacaaac cggttcgact ccgctgctgc tttcactggc
     9421 ggtcaacgcc gacagtctgg cgacgtggtt gcttccggca ctggctcctg tgttggctga
     9481 ttcgcctatc cgcctcaact tgcaggtaga agatgaaacc cgtactcagg aacgtctgcg
     9541 ccgcggcgaa gtggtcggcg cggtgagtat tcaacatcag gcgctgccga gttgtcttgt
     9601 cgataaactt ggtgcgctcg actatctgtt cgtcagctca aaaccctttg ccgaaaaata
     9661 tttccctaac ggcgtaacgc gttcggcatt actgaaagca cctgtggtcg cctttgacca
     9721 tcttgacgat atgcaccagg cctttttgca gcaaaacttc gatctgcctc caggcagcgt
     9781 gccctgccat atcgttaatt cttcagaagc gttcgtgcaa cttgctcgcc agggcaccac
     9841 ctgctgtatg atcccgcacc tgcaaatcga gaaagaacta gccagcggtg aactgattga
     9901 cttaacgcca gggctatttc aacgccggat gctctactgg caccgctttg ctcctgaaag
     9961 ccgcatgatg cgtaaagtca ctgatgcgtt gctcgattat ggtcacaaag tccttcgtca
    10021 ggattaatcc atcaaataat gcctgatagc acatatcagg cgttatcctc acttcttttt
    10081 gtattccttg aatcacatca caaaatagac aaatctcagg cggcaaaaaa cgacgtctga
    10141 atgcattttt ttgctggcga caaacccacg taaaaagctc accgtaagcg caaataccct
    10201 cattttgatt gcgttttacg gagcaaataa tgtctaacga gcaggagtgg caacagcttg
    10261 ccaacaagga attgagccgt cgggagaaaa ctgtcgactc gctggttcag caaaccgcgg
    10321 aagggatcgc catcaagcag ctgtataccg aagccgatct cgataatctg gaggtgacag
    10381 gtacccttcc tggtttgccg ccctacgttc gtggcccgcg tgccactatg tataccgccc
    10441 aaccgtggac catccgtcag tatgctggtt tttcaacagc aaaagagtcc aacgcttttt
    10501 atcgccgtaa cctggccgcc gggcaaaaag gtctttccgt tgcgtttgac cttgccaccc
    10561 accgtggcta cgactccgat aacccacgcg tggcgggtga cgtcggcaaa gcgggcgtcg
    10621 ctatcgacac cgtggaagat atgaaagtcc tgttcgacca gatcccgctg gataaaatgt
    10681 cggtttcgat gaccatgaat ggcgcagtgc taccagtact ggcgttttat atcgtcgccg
    10741 cagaagagca aggtgttaca cctgataaac tgaccggcac cattcaaaac gatattctca
    10801 aagagtacct ctgccgcaac acctatattt acccaccaaa accgtcaatg cgcattatcg
    10861 ccgacatcat cgcctggtgt tccggcaaca tgccgcgatt taataccatc agtatcagcg
    10921 gttaccacat gggggaagcg ggtgccaact gcgtgcagca ggtagcattt acgctcgccg
    10981 atgggattga gtacatcaaa gcagcaatct ctgccggact gaaaattgat gacttcgctc
    11041 ctcgcctgtc gttcttcttc ggtatcggca tggatctgtt tatgaacgtc gccatgttgc
    11101 gtgcggcacg ttatttatgg agcgaagcgg tcagtggatt tggcgcacag gatccgaaat
    11161 cactggcgct gcgtacccac tgccagacct caggctggag cctgactgaa caggatccgt
    11221 ataacaacgt tatccgcacc accattgaag cactggctgc gacgctgggc ggtactcagt
    11281 cactgcatac caacgccttt gacgaagcgc ttggtttgcc taccgatttc tcagcacgca
    11341 ttgcccgcaa cacccagatc atcatccagg aagaatcaga actctgccgc accgtcgatc
    11401 cactggccgg atcctattac attgagtcgc tgaccgatca aatcgtcaaa caagccagag
    11461 ctattatcca acagatcgac gaagccggtg gcatggcgaa agcgatcgaa gcaggtctgc
    11521 caaaacgaat gatcgaagag gcctcagcgc gcgaacagtc gctgatcgac caaggcaagc
    11581 gtgtcatcgt tggtgtcaac aagtacaaac tggatcacga agacgaaacc gatgtacttg
    11641 agatcgacaa cgtgatggtg cgtaacgagc aaattgcttc gctggaacgc attcgcgcca
    11701 cccgtgatga tgccgccgta accgccgcgt tgaacgccct gactcacgcc gcacagcata
    11761 acgaaaacct gctggctgcc gctgttaatg ccgctcgcgt tcgcgccacc ctgggtgaaa
    11821 tttccgatgc gctggaagtc gctttcgacc gttatctggt gccaagccag tgtgttaccg
    11881 gcgtgattgc gcaaagctat catcagtctg agaaatcggc ctccgagttc gatgccattg
    11941 ttgcgcaaac ggagcagttc cttgccgaca atggtcgtcg cccgcgcatt ctgatcgcta
    12001 agatgggcca ggatggacac gatcgcggcg cgaaagtgat cgccagcgcc tattccgatc
    12061 tcggtttcga cgtagattta agcccgatgt tctctacacc tgaagagatc gcccgcctgg
    12121 ccgtagaaaa cgacgttcac gtagtgggcg catcctcact ggctgccgat cataaaacgc
    12181 tgatcccgga actggtcgaa gcgctgaaaa aatggggacg cgaagatatc tgcgtggtcg
    12241 cgggtggcgt cattccgccg caggattacg ccttcctgca agagcgcggc gtggcggcga
    12301 tttatggtcc aggtacacct atgctcgaca gtgtgcgcga cgtactgaat ctgataagcc
    12361 agcatcatga ttaatgaagc cacgctggca gaaagtattc gccgcttacg tcagggtgag
    12421 cgtgccacgc tcgcccaggc catgacgctg gtggaaagcc gtcacccacg tcatcaggcg
    12481 ctaagtacac agctgcttga cgccattatg ccgttctgtg gtaacgccct gcgactgggc
    12541 gttaccggca cacccggcgc ggggaaaagt acctttcttg aagcctttgg catgttgttg
    12601 attcgtgagg gattaaaggt tgctgttatt gcggtcgatc ccagcagccc ggtcactggc
    12661 ggtagcattc tcggggataa aacccgcatg aatgacctgg cgcgtgccga ggcggcgttt
    12721 attcgcccgg taccctcctc cggtcatctg ggcggtgcca gtcagcgagc acgggaatta
    12781 atgctgttat gcgaagcggc gggttatgac gtagtgattg tcgaaacggt tggcgtcggg
    12841 cagtcggaaa cggaagtcgc ccgcatggtg gactgtttta tctcgttgca aattgccggt
    12901 ggcggcgatg atctgcaggg cattaaaaaa gggctgatgg aagtggctga tctgctcgtt
    12961 atcaacaaag acgatggcga taaccatacc aatgtcgcca ttgcccggca tatgtacgag
    13021 agtgccctgc atattctgcg acgtaaatac gacgaatggc agccacgggt tctgacttgt
    13081 agcgcactgg aaaaacgtgg aatcgatgag atctggcacg ccatcatcga cttcaaaacc
    13141 gcgctaactg ccagtggtcg tttacaacaa gtgcggcaac aacaatcggt ggaatggctg
    13201 cgtaagcaga ccgaagaaga agtactgaat cacctgttcg cgaatgaaga tttcgatcgc
    13261 tattaccgcc agacgctttt agcggtcaaa aacaatacgc tctcaccgcg caccggcctg
    13321 cggcagctca gtgaatttat ccagacgcaa tattttgatt aaaggaattt ttatgtctta
    13381 tcagtatgtg aacgttgcca ctatcaacaa agtggcggtc attgagttta actatggacg
    13441 aaaacttaat gccttaagta aagtctttat tgatgatctt atgcaggcgt taagcgatct
    13501 caaccggccg gaaattcgct gtatcatttt gcgggcgcca agtggatcca aagtcttctc
    13561 cgcaggtcac gatattcacg aactgccgtc tggcggtcgc gatccgctct cctatgatga
    13621 tccattgcgt caaatcaccc gcatgatcca aaaattcccg aaaccgatca tttcgatggt
    13681 ggaaggtagt gtttggggtg gcgcatttga aatgatcatg agttccgatc tgatcatcgc
    13741 cgccagtacc tcaaccttct caatgatgcc tgtaaacctc ggcgtcccgt ataacctggt
    13801 cggcattcac aacctgaccc gcgacgcggg cttccacatt gtcaaagagc tgatttttac
    13861 cgcttcgcca atcaccgccc agcgcgcgtt ggctgtcggc atcctcaacc atgttgtgga
    13921 agtggaagaa ctggaagatt tcaccttaca aatggcgcac cacatctctg agaaagcgcc
    13981 gttagccatt gctgttatca aagaagagct gcgtgtactg ggcgaagcgc acaccatgaa
    14041 ctccgatgaa tttgaacgta ttcaggggat gcgccgcgcg gtttatgaca gcgaagatta
    14101 ccaggaaggg atgaacgctt tcctcgaaaa acgtaaacct aatttcgttg gtcattaatc
    14161 cctgcgaacg aaggagtaaa aatggaaact cagtggacaa ggatgaccgc cgatgaagcg
    14221 gcagaaatta tccagcataa cgacatggtg gcatttagcg gctttacccc agcgggttcg
    14281 ccgaaagcct taccgaccgc gattgcccgc agagctaacg aacagcatga ggccaaaaag
    14341 ccgtatcaaa ttcgcctgct gacgggggcg tcaatcagcg ccgccgctga cgatgtactt
    14401 tctgacgccg atgctgtttc ctggcgtgcg ccatatcaaa catcgtccgg tttacgtaaa
    14461 aagatcaatc agggcgcggt gagtttcgtt gacctgcatt tgagcgaagt ggcgcaaatg
    14521 gtcaattacg gtttcttcgg cgacattgat gttgccgtca tcgaagcatc ggcactggca
    14581 ccggatggtc gagtctggtt aaccagcggg atcggtaatg cgccgacatg gctgctgcgg
    14641 gcgaagaaag tgatcattga acttaatcac tatcacgatc cgcgcgttgc agaactggcg
    14701 gatattgtga ttcctggcgc accaccgcgg cgcaatagcg tgtcgatctt ccatgcaatg
    14761 gatcgcgtcg gtacccgcta tgtgcaaatc gatccgaaaa agattgtcgc cgtcgtggaa
    14821 accaacttgc ccgacgccgg taatatgctg gataagcaaa atcccatgtg ccagcagatt
    14881 gccgataacg tggtcacgtt cttattgcag gaaatggcgc atgggcgtat tccgccggaa
    14941 tttctgccgc tgcaaagtgg cgtgggcaat atcaataatg cggtaatggc gcgtctgggg
    15001 gaaaacccgg aaattcctcc gttcatgatg tattcggaag tgctacagga atcggtggtg
    15061 catttactgg aaaccggcaa aatcagcggg gccagcgcct ccagcctgac aatctcggcc
    15121 gattccctgc gcaagattta cgacaatatg gattactttg ccagccgcat tgtgttgcgt
    15181 ccgcaggaga tttccaataa cccggaaatc atccgtcgtc tgggcgtcat cgctctgaac
    15241 gtcggcctgg agtttgatat ttacgggcat gcaaattcaa cgcacgtagc cggggtcgat
    15301 ctgatgaacg gcatcggcgg cagcggtgat tttgaacgca acgcgtatct gtcgatcttt
    15361 atggctccgt cgattgctaa agaaggcaag atctcaaccg tcgtgccaat gtgcagccat
    15421 gttgatcaca gcgaacacag cgtcaaagtg atcatcaccg aacaagggat cgccgatctg
    15481 cgcggtcttt ccccgcttca acgcgcccgc actatcattg ataattgtgc acatcctatg
    15541 tatcgggatt atctgcatcg ctatctggaa aatgcgcccg gcggacatat tcaccacgat
    15601 cttagccacg tcttcgactt acaccgtaat ttaattgcaa ccggctcgat gctgggttaa
    15661 tggtttgtcc cgtcacgatc ttcagcaata tgctgaagca tcgtggcggt ataacgatga
    15721 tttctgattg aataaagata ttcctgcata tacatcggcc tgtgcggcgc atcaaaatat
    15781 ttcagcgttg ccggattcac cagtcgccag gcaaaatgcg ggatcaccgt cagaaaatgt
    15841 ccgcgctcca cggcgctgat tttggccata aaactgtagg gacgataaat aatggtggga
    15901 ttgatcccac aaggtcgcat attcgcatca atcattgctt cgaagttagc ccggttttga
    15961 aagcgcattt gtagccaggg cagctcctga agcagctctt gctcttgacg ctcttcataa
    16021 cggcgagaaa cgagaaagcc caatcgcagc ggtggtaatt cactgatagt caggttttcc
    16081 agttcctgaa ctcgtgcaga tacatgctgt ggggagataa taaaatctaa ctgacggtca
    16141 aaaagattat cgataacgcc gttttcgctg aactcaactg gctgggcggt cacgccttca
    16201 tacttatctc cgagactaat caactgatcg aaaataattg tcggataagt attatctatg
    16261 ccaatcacaa tt
//
LOCUS       DAZPJR010000088        15961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPJR010000088 DAZPJR010000000
VERSION     DAZPJR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.089"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHVB7_002625"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHVB7_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR1914573.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHVB7_002626"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHVB7_002626"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR1914574.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHVB7_002627"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHVB7_002627"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR1914575.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHVB7_002628"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHVB7_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHVB7_002629"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHVB7_002629"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR1914576.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHVB7_002630"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHVB7_002630"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR1914577.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="ACHVB7_002631"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="ACHVB7_002631"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR1914578.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="ACHVB7_002632"
     CDS             8229..8939
                     /locus_tag="ACHVB7_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1914579.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHVB7_002633"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHVB7_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR1914580.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="ACHVB7_002634"
     CDS             9636..10343
                     /locus_tag="ACHVB7_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1914581.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="ACHVB7_002635"
     CDS             10343..10891
                     /locus_tag="ACHVB7_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR1914582.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="ACHVB7_002636"
     CDS             10901..12067
                     /locus_tag="ACHVB7_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1914583.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="ACHVB7_002637"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="ACHVB7_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13576..14229
                     /locus_tag="ACHVB7_002638"
     CDS             13576..14229
                     /locus_tag="ACHVB7_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1914584.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHVB7_002639"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHVB7_002639"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR1914585.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="ACHVB7_002640"
     CDS             complement(15612..>15961)
                     /locus_tag="ACHVB7_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1914586.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3700 a         4345 c         4162 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAZPJR010000089        15926 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPJR010000089 DAZPJR010000000
VERSION     DAZPJR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.090"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..168
                     /locus_tag="ACHVB7_002641"
     CDS             <1..168
                     /locus_tag="ACHVB7_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1914587.1"
                     /translation="NRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFG
                     MTPGEYRRKFR"
     gene            complement(340..669)
                     /locus_tag="ACHVB7_002642"
     CDS             complement(340..669)
                     /locus_tag="ACHVB7_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR1914588.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(674..859)
                     /locus_tag="ACHVB7_002643"
     CDS             complement(674..859)
                     /locus_tag="ACHVB7_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR1914589.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(856..3495)
                     /locus_tag="ACHVB7_002644"
     CDS             complement(856..3495)
                     /locus_tag="ACHVB7_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR1914590.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3703..4692
                     /gene="ldhA"
                     /locus_tag="ACHVB7_002645"
     CDS             3703..4692
                     /gene="ldhA"
                     /locus_tag="ACHVB7_002645"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1914591.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4803..5225
                     /gene="hslJ"
                     /locus_tag="ACHVB7_002646"
     CDS             4803..5225
                     /gene="hslJ"
                     /locus_tag="ACHVB7_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR1914592.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5222..5488)
                     /locus_tag="ACHVB7_002647"
     CDS             complement(5222..5488)
                     /locus_tag="ACHVB7_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR1914593.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5762..9286
                     /gene="nifJ"
                     /locus_tag="ACHVB7_002648"
     CDS             5762..9286
                     /gene="nifJ"
                     /locus_tag="ACHVB7_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR1914594.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9652..10785
                     /gene="ompN"
                     /locus_tag="ACHVB7_002649"
     CDS             9652..10785
                     /gene="ompN"
                     /locus_tag="ACHVB7_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR1914595.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10926..11360
                     /gene="uspF"
                     /locus_tag="ACHVB7_002650"
     CDS             10926..11360
                     /gene="uspF"
                     /locus_tag="ACHVB7_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR1914596.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11946..12860
                     /gene="sitA"
                     /locus_tag="ACHVB7_002651"
     CDS             11946..12860
                     /gene="sitA"
                     /locus_tag="ACHVB7_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR1914597.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12860..13687
                     /gene="sitB"
                     /locus_tag="ACHVB7_002652"
     CDS             12860..13687
                     /gene="sitB"
                     /locus_tag="ACHVB7_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR1914598.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13684..14541
                     /gene="sitC"
                     /locus_tag="ACHVB7_002653"
     CDS             13684..14541
                     /gene="sitC"
                     /locus_tag="ACHVB7_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR1914599.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14538..15395
                     /gene="sitD"
                     /locus_tag="ACHVB7_002654"
     CDS             14538..15395
                     /gene="sitD"
                     /locus_tag="ACHVB7_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR1914600.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15596..>15926)
                     /locus_tag="ACHVB7_002655"
     CDS             complement(15596..>15926)
                     /locus_tag="ACHVB7_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1914601.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
BASE COUNT         3984 a         4022 c         3829 g         4091 t
ORIGIN      
        1 aaccgtcgtc tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg
       61 gcaggcatcg gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag
      121 caacgctttg ggatgacgcc aggcgagtat cgacgtaaat tccgttaact aattcgcgtt
      181 gtgtgtactg caacgccaat tagttaaaac agatatttga ctatgcattt ttcagggcaa
      241 aaggcaattt gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc
      301 tttctcctgt agcgttatcc cgatattgcg gttttgctat taaaattttc gcacccattt
      361 accgtttatc ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc
      421 gagtttcgcg atatcatcta ccgacacatt attttgcttt gccagttgtt ggtaactcgc
      481 tttacgttcg gcattaatgt cggttactaa tttctctgtt tcagcatccg ttttcagcgc
      541 aaccagataa ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt
      601 caatgccatt acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt
      661 cttcatcatc gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt
      721 ctgccttgat gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct
      781 tgggcgcagc gacttcaata cgcggcgtac agccaaccag cataaaagat gacgtcaacg
      841 cagccagtaa aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc
      901 cggcagacgt gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg
      961 ccacagcgta aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc
     1021 atcaacgcga ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa
     1081 gttaattttt gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc
     1141 cgcagtcata ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat
     1201 tggcccgcta tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa
     1261 cggcaatgca ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac
     1321 cagttcgctg gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg
     1381 taattgtttc atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa
     1441 gggttcttct tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt
     1501 caccagatta atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca
     1561 ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg
     1621 catccaggca ctaccgcctt tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg
     1681 cgcagcagaa aatgcaacct gagcatacag ttccccatcg cgtaagttca tcttccagtc
     1741 gggtggcacc agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg
     1801 cagacgaata ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa
     1861 atcgccttta aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga
     1921 tggcggtaac acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc
     1981 gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg
     2041 cggcttttct accgtcatcg taccgtattg taactggtcg aaaccggtgg agaggtcggt
     2101 cagcgtaatc gtgctgtcat gccattcacc ttttcctgcg acatcccagg tggcatttat
     2161 cggtgtaaaa ctgccttttc cccagtagcg ccactgccag cggcccgcgt cagggagaaa
     2221 atcattcgcc agcccatcca tatgcagcac gaaatcgccc agttcatatt catgcgcctg
     2281 caaaatggcc tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg
     2341 ccagcggatt tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc
     2401 gcctggctca aaggccagcg ttgggtcagt cagacttcca cttagctgcg cgggtaagcg
     2461 ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct
     2521 gttatccatg cttaattttc ctgggccaaa atttagcacc gcgttgcctt tacccgcttg
     2581 cccctgggtc agcacactca gtcgtccgct gatcagagcg ttctcaagcc ctgcctgcca
     2641 gttgtcgact ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccaactcca
     2701 gcgaccatcg cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg
     2761 atcgccgtta tcccgtgcca gcacaatcaa ttgcccgcta ttttcctgcc agtccagctc
     2821 tgcatccacc agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac
     2881 aggaagtcca tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg
     2941 attttcaaac gcgatgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc
     3001 ttgaaattta actttttcgc cctgataacg cagttgctga atatcagagg ttaatgcgag
     3061 agagagtttt ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca
     3121 ggtgttaggc agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc
     3181 cgtctgcggc aatttcgcca gacaagcaga atcaagttct accatgccga cgttcaataa
     3241 ccagcggctg ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac
     3301 cagataacgg agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa
     3361 tgcaatacgt gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag
     3421 cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt
     3481 atatttaccc agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac
     3541 gggtattgtg gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcattgttgt
     3601 tgatgaattt ttcaatatcg ccatagcttt caattatatt tgaaattttg taaaatattt
     3661 ttagtagctt aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat
     3721 agcacaaaac agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag
     3781 ctggaatttt ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa
     3841 gcggtatgta ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag
     3901 cacggcgtta aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg
     3961 gcaaaagaac tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct
     4021 gaacacgcca tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt
     4081 acccgtgacg ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg
     4141 gcaggcgtta tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt
     4201 ggtatgcgtc tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg
     4261 gagtatgtcg atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg
     4321 ctgacaccgg aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc
     4381 gtgatgatcg tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg
     4441 ctgaaaaatc agaaaattgg gtcgttgggt atggacgtct atgagaacga acgcgatcta
     4501 ttctttgaag ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc
     4561 tgccacaacg tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt
     4621 atttctcaga ctacgctgca aaacttaagc aatctggaaa aaggcgaaac ctgcccgaac
     4681 gaactggttt aatcttgccg ctcccctgca ttccagggga gcttattcag ataatcccca
     4741 aagacctttc atcttctatt cttaaaatag ccctgagtca gaaactgtaa ttgagaacca
     4801 caatgaagaa agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta
     4861 atgacaaaat tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg
     4921 taaacggtaa gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa
     4981 tgatgatttc cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg
     5041 aactgacagc caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg
     5101 aactcgataa caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga
     5161 accagttaac gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga
     5221 attaatagct gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg
     5281 gtaatgcaca catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc
     5341 cgatcattgc acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta
     5401 cgtgcgccgc agtcgcctgc tgaacaggtt cactactaca cgccgacaac aataaagcgg
     5461 cacaccctac ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac
     5521 accgccataa ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc
     5581 cgcattttta cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac
     5641 gctgatgtgg gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaagg
     5701 attactactg ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca
     5761 tatgattact attgatggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt
     5821 tatcgccatc taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc
     5881 cggaaacggc ttgaagaacg tttggggaga cacaccatgc gtggttgaaa tgcagtcgga
     5941 agcgggtgct atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt
     6001 tacgtcatcg cagggtttgc tgctgatgat cccgacactg tacaaactgg caggcgaact
     6061 cacaccgttt gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt
     6121 tggcgatcat tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc
     6181 aaacgtccag gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg
     6241 cgtgccattt attcatttct ttgatggttt ccgcacgtcc cacgaaatca acaaaattgt
     6301 cccgctggcc gatgacacga ttcttgatct catgccgcag gccgaaattg atgctcatcg
     6361 cgcccgggcg ctcaacccgg aacatccggt gatccgtggt acgtccgcca atcctgacac
     6421 ttatttccag tctcgcgaag ccaccaaccc ctggtacaac gcagtctatg accatgttga
     6481 acaggcgatg aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta
     6541 cgggcatccg caagctgaac gggtgattat cctgatgggc tcagccattg gcacctgtga
     6601 agaagtggtt gatgaattgc taacccgtgg cgaaaaagtc ggcgtgctga aagttcgcct
     6661 gtaccgcccc ttctcagcca aacacttgct acaaactcta ccgggatccg tacgcagcgt
     6721 ggcggtactg gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt
     6781 gatgaccgca ctggcagaag cctttaataa tggcaagcgc gaaactctgc cccgtgtcat
     6841 tggtgggcgc tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt
     6901 tgccgagctc aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt
     6961 aaccaatctg tccctgccgt tgccggaaaa cacccagcca aactcggcga aacttgaagc
     7021 cttgttttat ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat
     7081 tatcggtaat tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaagc
     7141 gggcggcctg acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct
     7201 catttcccag gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat
     7261 ggctgagcgt ttaaaacctg gcggcatttt cctgctcaac acgccgtaca gcgcagatga
     7321 agtgtggtcg cgcttgccgc aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt
     7381 ctatgtgatt aacgcggcga aaatcgcccg caaatgtggc ctggcggccc gtattaatac
     7441 cgtcatgcag atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc
     7501 agaattgcag ggtgcgattg ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg
     7561 caactggcag gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt
     7621 aaatccgcac agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa
     7681 aaccgtaacc gcagcgatgc tcgcagggct tggtgatgcc ctccccgttt cggcgctgcc
     7741 gccagacggc acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga
     7801 gatccccatc tggaaagagg aactctgtac ccaatgtaac cactgcgtcg cagcttgccc
     7861 acactcagct attcgcgcaa aagtggtacc acccgaagcg atggaaaacg cccctgccag
     7921 cctgcattcg ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt
     7981 ggctccggaa gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg
     8041 tcagaatcca gagattaaag ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga
     8101 gaaaatcaat tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg
     8161 tattgatatt cgtacatcgc agctgattac accgctgttt gaatattccg gtgcttgctc
     8221 cggttgtggc gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt
     8281 gatcgctaac gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta
     8341 taccaccgat gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc
     8401 cgaatttggc cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct
     8461 gctggatcaa tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc
     8521 tacgccagag gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc
     8581 cgaagcacat gaactgctac gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat
     8641 tggtggtgat ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt
     8701 gacggaaaac gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca
     8761 ggcgtcgaaa gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa
     8821 agcgcgtaaa gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat
     8881 ttctctcggc gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc
     8941 gggaccatcg ctgatcattg cttatagccc gtgtgaagag catggttacg atctggcact
     9001 cagccatgac cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga
     9061 tcctcgtcgt gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga
     9121 agcactggaa gaaacgttac ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc
     9181 agaagtggca gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt
     9241 cctggcacaa atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag
     9301 gattacccaa ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg
     9361 gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa taaaggcata
     9421 taacccgcgc agaataacgt atagaaaatt aaaagtataa tttttatttt gtatgtatta
     9481 tcttcgatac attcaccaga cttattatca tttcagaaga attatttgat taaggtttta
     9541 cttaaggcgt aacaaatgat atttatcggc taactgaact tctcctttag gatgttttca
     9601 ctccccggaa tggggataaa aggcaaataa aataactaaa ggatttattc aatgaaaagc
     9661 aaagtactgg cacttttaat tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa
     9721 gtttataata aagacggcaa caaattagat ctgtatggca aagttgatgg cctgcattat
     9781 ttttctgata attcagcgaa agatggcgat cagagctatg cgcgtctggg ttttaaaggc
     9841 gaaacccaaa ttaacgatca actcactggc tacggtcaat gggaatacaa tattcaggca
     9901 aacaacactg aatcttcaaa aaaccagtca tggacccgtc tggcatttgc cgggctgaaa
     9961 tttgcagatt acggttcttt cgattacgga cgtaattatg gcgtaatgta cgacatcgaa
    10021 ggctggaccg atatgctgcc tgaatttggc ggtgactctt ataccaatgc agacaacttt
    10081 atgactggtc gtgccaacgg cgtcgcgact tatcgtaata ctgatttctt cggtctggta
    10141 aatggtctga acttcgcggt gcagtatcaa ggtaacaacg aaggtgccag taatggtcag
    10201 gaaggcacca acaacggacg tgatgttcgc catgaaaatg gtgacggctg gggtctttcc
    10261 acaacatatg atttaggcat gggctttagc gctggtgcgg catacacctc atctgaccgc
    10321 accaatgacc aggttaacca tactgcggcg ggtggtgata aagcagacgc gtggactgct
    10381 gggctaaaat acgatgctaa caatatttac ctggcaacca tgtattcaga aacgcgtaat
    10441 atgaccccgt ttggcgacag cgattatgct gttgcaaaca aaacccagaa ctttgaagtc
    10501 actgcacagt accagtttga ttttggtctg cgtccggcag tctctttcct gatgtctaaa
    10561 ggccgtgacc tgcacgctgc cgatggtgca gacaacccgg caggtgttga tgataaagat
    10621 cttgttaaat acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacttat
    10681 gttgactata aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc
    10741 tctaccgatg atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct
    10801 gttatggcgg gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact
    10861 ccgtcattac acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag
    10921 gttttatgaa cagaacgatt cttgtcccta tcgatatttc cgattcagaa ttaactcaac
    10981 gcgtgattag ccacgttgag gcagaggcaa agattgacga tgcagaggtt catttcctga
    11041 cggtaatacc ttcactgcct tactatgcct ctctgggttt agcgtattcc gcagaattac
    11101 cggcaatgga tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat
    11161 ttaaactgcc aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca
    11221 ttctggaatt ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg
    11281 atatcaccac ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct
    11341 ccgtgctggt tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt
    11401 tcgcaaatgt gctgacattt tcccctctaa tccgtaccat acacgccaca gttttatatc
    11461 agatttctta tgctgggcgt tccggcatga cgcatactct tctgatgcca tataacgaat
    11521 tgagtcgctt ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga
    11581 acaagtaagg gcaaaaatca caactatctg acccataagt attttattta tttttcaata
    11641 tgttaaacat tatcagcgac aacaataagc tacgataacc cactcttttt ttgccccttt
    11701 tttgcccctt ctacagcgtt ttgccccatt tttgccatca acaattttcc tcataaagtc
    11761 atttcaaagc ctaatagctg ctctactttt ttcctcaata tcatgtcgcc agcaggaaac
    11821 aacctaatcc ctgttttagg cctcaaagct gttaatgata atcattttca ttttgtatgg
    11881 gttatgctat aaaacatagc cgttgctatg tttgttctaa ttttgctcac tataggtact
    11941 aaattatgca ctcaataaaa aaagtaacca tgctcttggg gtgcctcgcc ctcacctgct
    12001 cgatcgcatt tcaggcaagt gcaactgaaa aattcaaggt cattacaaca tttaccatca
    12061 tcgcggatat ggccaaaaac gtggctggag atgctgcaga agtctcatcc ataaccaagc
    12121 ctggtgcaga aattcatgag tatcagccta cccctggcga tattaaacgt gcgcaggggg
    12181 cacaactgat tctcgccaat ggtatgaatc tggaattgtg gttccaacgc ttttaccagc
    12241 atctcaatgg ggttccagaa gtaattgtct cttcgggtgt gacgccagta gggatcaccg
    12301 aaggacccta tgagggcaaa cctaatcccc atgcatggat gtcgccagat aatgctctga
    12361 tttacgtcga taatattcgt gatgcgttga taaaatacga tccggcaaat gcacaaacct
    12421 accaacgtaa tgccgatact tataaagcca agattaccca aacccttgcc cccctgcgta
    12481 agcagattac ggaactccct gagaatcagc gatggatggt caccagtgaa ggggcttttt
    12541 cttatctcgc acgcgatttg gggctaaaag agctttatct gtggccgatt aatgccgatc
    12601 aacaaggaac accgcagcag gtacgtaagg ttgttgatat agttaagaaa aatcatatcc
    12661 cggcagtctt tagcgagagt acgatttccg ataaaccagc gcgtcaggtt gcgcgtgaaa
    12721 ccggcgcaca ctacggcggt gtgctctatg tcgattccct gagcacagaa aacggcccgg
    12781 tacctacgta tatcgacctt cttaaggtta ctaccagtac gctggtacag ggaattaaag
    12841 ccgggaaaag ggagaaataa tgatgcaatc tgcaggcatt gtcgttaatg atgttactgt
    12901 cacctggcgt aacgggcaca cagcactgcg cgatgcatcc ttcacagtac caagtggatc
    12961 tattgccgct ctggttgggg taaacggttc cggaaaatcg acgttgttta aagcgattat
    13021 gggatttgtg cgtctgacca gcggaaagat atctgttctg ggtattccca cgcgacaggc
    13081 gctacagaaa aacctggttg cctacgtccc tcagtcagaa gaggttgact ggtcatttcc
    13141 tgtactggtg gaagatgtgg tgatgatggg ccgctatggg catatgggta tgttgcgtat
    13201 agccaaaaag agggatcgtc agattgtcac cgatgcgctg gaacgtgtcg atatggtgga
    13261 ttttcgccat cgtcaaatcg gcgagctttc cggtggacaa aagaaacgcg tctttctggc
    13321 gagagcgatt gcacagcagg gtgatgtgat cctgctggat gaacctttta ccggcgttga
    13381 tgtgaaaacc gaagcgaaaa tcatcagcct gttgagggag ttacgcgcgg aaggcaaaac
    13441 gatgcttgtc tcaacgcaca atctcggctc agtcacgaca ttttgcgatt atacggtcat
    13501 ggtcaaaggc accgtcctgg caagcgggcc gacagacacc acttttacag ccgagaacct
    13561 ggagctggct tttagcggcg tactgcgcca tgtcacactt aacgactcgg aagaaagcat
    13621 tattactgac gatgaacgcc cttttgtggc acatcgaccg tcagcggtgc agagggaagc
    13681 aagatgaacg tgctactgga acccttcagc tatgaatata tgctcaatgc gatgtgggtc
    13741 tcggcaatgg tcggcggtct ctgcgctttc ctgtcatgct atctgatgct aaaaggctgg
    13801 tcgctgattg gcgatgcgct gtcgcactct attgtcccgg gtgttgcggg ggcatatatg
    13861 ctggggcttc cgttttctct gggagccttc ttttccggcg gactggcagc gggcagcatg
    13921 ctgtttctta accaacgcac ccgcttaaaa gaagatgcca tcatcggcct gatcttctcg
    13981 tccttttttg gcctggggct gtttatggtg tcattgaacc cgacatccgt gaacattcag
    14041 accatcgtcc tgggcaatat tctggcaatc gatccggcgg atatcctgca actcactatc
    14101 attggcattc tctcgatcat cgtgcttttt ttcaaatgga aagatcttat ggtgaccttt
    14161 ttcgacgaga atcacgcccg tgctattgga ttacatccgg gcagattaaa aatcctcttc
    14221 ttcaccctgc tgtcggtttc gaccgttgcg gcgctgcaaa cggttggtgc ctttctggtg
    14281 atctgtctgg tggtgactcc cggtgcaaca gcatggttgt taaccgatcg ttttccccgc
    14341 ctgctcataa ttgctgtaac tattggcagt gtaaccagtt tcctcggcgc gtgggtcagt
    14401 tactttctgg acggcgcgac aggtggaatc atcgtggtgg cgcagacact gctgtttctt
    14461 ttagcatttg tatttgcgcc cacccatgga cttttggcaa atcgtcggcg tgcgcataaa
    14521 gcgctggagg atcgctcatg atggcattat tactggagcc actgcagttc acctttatga
    14581 gtcacgcact gctgatttca ctggtagttt ccataccctg tgcgctgctg tcggtctttc
    14641 tggtcctgaa aggttgggca ctgatgggtg acgcgatgag tcatgcggtt tttcctggca
    14701 tcgtgcttac ctggattctg gggttacctc tggctacagg ggcattcgtc gccggagtat
    14761 tctgcgcggt ggcgacggga tacctgaaag acaacagccg aatcaagcaa gatacagtta
    14821 tggggattgt tttttctggc atgtttgccg caggtctgat cttgtatatt gcaatcaaac
    14881 cagacgtaca tcttgaccat attcttttcg gcgacatgct cgggataacc atcggcgata
    14941 taatccagac ggtgattatt gccgggctgg ttacacttgt tattagcgca aaatggcggg
    15001 attttttgct gttcagcttt gattatcaac aggcgcaggt aagcggtttg catacgagat
    15061 ggctgcatta tgggctgctg tgcatggttt ccctgaccat tgtggcgacg ctgaaagcag
    15121 taggtatcat tctctctatt tctctgctca ttgcgcctgg cgccattgca gtactcctga
    15181 cacaacgctt tcatattgcg ctcctactgg cgactggcat atcggtaata gtgtcaatga
    15241 ctggtgtctg gctgtccttt tttatcgaca gcgcaccagc tccgacgatt gtcgtcttat
    15301 tcgcggtcgt gttcatcatg acgtttaccg ttaccagcat caacgcacgc acaaagggaa
    15361 acgcccatac acaggatctg ttatcaccca attagcacaa catccgagga cagcatcgtc
    15421 cgccagccta cagaagtccg gaaagccatg gaaggtcgtt gggaggaggt acgaattcta
    15481 tcatgcaaaa aatacgtaaa atcgataacg cctagaaatc attcaatact cgcactatcg
    15541 aaagtttacc agccaaccgc ggcacgttcc tgcatacgac gtgccgcagc ttctgttatt
    15601 ccacgataaa attcctaacc gaattcaaat ccttcagact attcagctct tccttcagct
    15661 cccgctgacg gcgataaatc tcgtcattgc gctcaacctg tgcctgagcc attgctgccg
    15721 ccagttcttc cagttccggc atcgactgtt tcacctgctg attatcggca tcaccccacg
    15781 tcatcacgtc ccgtacaatg tcggatttcg cagccattac caccggataa aggcggccca
    15841 gtgagttggg gccagcattc cagatacgac cgttccattc aaacgtgaac ggcttcgcct
    15901 cctgttctgt gcgccatgct tcaatt
//
LOCUS       DAZPJR010000090        15582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPJR010000090 DAZPJR010000000
VERSION     DAZPJR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.091"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..2065
                     /locus_tag="ACHVB7_002656"
     CDS             <1..2065
                     /locus_tag="ACHVB7_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR1914602.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2081..3115
                     /locus_tag="ACHVB7_002657"
     CDS             2081..3115
                     /locus_tag="ACHVB7_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR1914603.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="ACHVB7_002658"
     CDS             complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="ACHVB7_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR1914604.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="ACHVB7_002659"
     CDS             complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="ACHVB7_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR1914605.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4699..4971
                     /gene="rcnR"
                     /locus_tag="ACHVB7_002660"
                     /pseudo
     CDS             4699..4971
                     /gene="rcnR"
                     /locus_tag="ACHVB7_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5063..5159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5194..5982
                     /gene="thiM"
                     /locus_tag="ACHVB7_002661"
     CDS             5194..5982
                     /gene="thiM"
                     /locus_tag="ACHVB7_002661"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR1914606.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5979..6779
                     /gene="thiD"
                     /locus_tag="ACHVB7_002662"
     CDS             5979..6779
                     /gene="thiD"
                     /locus_tag="ACHVB7_002662"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR1914607.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6844..7662
                     /locus_tag="ACHVB7_002663"
     CDS             6844..7662
                     /locus_tag="ACHVB7_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR1914608.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7714..8460
                     /locus_tag="ACHVB7_002664"
     CDS             7714..8460
                     /locus_tag="ACHVB7_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1914609.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8434..9399)
                     /locus_tag="ACHVB7_002665"
     CDS             complement(8434..9399)
                     /locus_tag="ACHVB7_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR1914610.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9396..10400)
                     /locus_tag="ACHVB7_002666"
     CDS             complement(9396..10400)
                     /locus_tag="ACHVB7_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR1914611.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10397..11674)
                     /locus_tag="ACHVB7_002667"
     CDS             complement(10397..11674)
                     /locus_tag="ACHVB7_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR1914612.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11931..12983
                     /gene="fbaB"
                     /locus_tag="ACHVB7_002668"
     CDS             11931..12983
                     /gene="fbaB"
                     /locus_tag="ACHVB7_002668"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR1914613.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13302..14156
                     /gene="gatY"
                     /locus_tag="ACHVB7_002669"
     CDS             13302..14156
                     /gene="gatY"
                     /locus_tag="ACHVB7_002669"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR1914614.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14185..15442
                     /gene="gatZ"
                     /locus_tag="ACHVB7_002670"
                     /pseudo
     CDS             14185..15442
                     /gene="gatZ"
                     /locus_tag="ACHVB7_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15452..>15582
                     /locus_tag="ACHVB7_002671"
     CDS             15452..>15582
                     /locus_tag="ACHVB7_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR1914615.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3728 c         3987 g         3779 t
ORIGIN      
        1 tggctatgtt ccaccggaaa actgggagcg gggtattaat gcgttttata cctcttatta
       61 tgtgagtcag tattacagtg actataaagc gtcgggtaat agtaagagta catatgtacg
      121 ttttaacagc gggttaaatt tactggggtg gcaactgcat tctgatgcca gtttcagtaa
      181 aacaaataac aatccagggg tgtggaaaag caataccctg tatctggaac gtggatttgc
      241 ccaacttctc ggcacgcttc gcgtgggtga tatgtacaca tcaagcgata tttttgattc
      301 tgttcgcttc agaggtgtgc ggttgtttcg tgatatgcag atgttgccta actcgaaaca
      361 aaattttacg ccacgggtgc aggggattgc tcagagtaac gcgctggtaa ctattgaaca
      421 gaatggtttt gtggtttatc agaaagaggt tcctcctggc ccgtttgcga ttacagattt
      481 gcagttggcc ggtggtggag cagatcttga tgtcagcgtg aaagaggcgg acggttcggt
      541 aaccacctat ctggtgcctt atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa
      601 atatgatttt gcggcgggtc gtagccatat tgaaggggcg agcaaacata gtgattttgt
      661 ccaggcgggt tatcagtatg gttttaataa cttattgacg ttgtatggtg gctcgatggt
      721 tgcgaataat tattacgcgt tcactttggg gactggctgg aatacacgca ttggtgccat
      781 ttccgtcgat gccacgaagt cgcatagtaa acaagacaac ggcgatgtgt ttgacgggca
      841 aagttatcaa attgcctaca acaaatttgt gagccaaacg tcgacgcgtt ttggtctggc
      901 ggcctggcgt tattcgtcgc gtgattaccg gacatttaac gatcacgttt gggcaaacaa
      961 taaagataat tatcgccgtg atgaaaacga tgtctatgac attgccgatt attaccagaa
     1021 tgattttggt cgcaaaaata gcttttccgc caatatgagc cagtcattgc cagaaggttg
     1081 ggggtctgtg tcattaagta cgttatggcg agattactgg gggcgtagcg gcagtagtaa
     1141 ggattatcag ttgagttatt ccaacaacct gcgacggata agctataccc tcgcggcaag
     1201 ccaggcttat gacgagaatc atcatgaaga gaaacgtttt aatattttta tatcgattcc
     1261 ctttgattgg ggtgatgacg tttcgacgcc tcgtcggcaa atatatatgt ctaactcaac
     1321 gacgtttgat gatcaggggt ttgcctcaaa taatacggga ttatcaggaa cagtagggag
     1381 tcgggatcag ttcaattatg gtgtcaacct gagtcatcaa catcagggaa atgaaacgac
     1441 agctggagcg aatttaacct ggaacgcgcc ggtggcaaca gtgaatggca gttatagtca
     1501 gtcgagtact tatcgacagg ctggagccag tgtttcaggg ggcattgtcg cctggtcggg
     1561 tggcgttaat ctggcgaacc gtctttccga aacgtttgct gtgatgaatg cgccaggaat
     1621 taaagatgct tatgtcaatg ggcaaaaata tcgcacaaca aaccgtaatg gagtggtggt
     1681 atacgacgga atgacacctt atcgggaaaa tcacctgatg ctggatgtgt cgcaaagcga
     1741 tagcgaagca gaattacgtg gcaaccggaa aattgccgca ccttatcgcg gcgcggttgt
     1801 actggttaat tttgataccg atcagcgcaa gccctggttt ataaaagcgt taagagcgga
     1861 tgggcaacca ttaacgtttg gttatgaagt caatgatatc cacggtcata atattggcgt
     1921 tgtcggccag ggaagccagt tatttattcg caccaatgaa ataccgccat cggttaatgt
     1981 agcaattgat aagcaacaag gactttcatg cacaatcacc ttcggtaaag agattgatga
     2041 aagtagaaat tatatttgcc agtaatagtc aggtggttct atggaaattc gcataatgct
     2101 atttatatta atgatgatgg ttatgcctgt gagctatgcg gcatgttata gtgagttatc
     2161 tgttcagcac aatttggttg ttcaggggga ttttgcactt actcaaacac aaatagcgac
     2221 atatgagcat aattttaatg attcgtcatg cgtgagtaca aatactatca cccctatgag
     2281 ttcgtcggat attattgttg ggctttataa cgataccata aaattaaatt tacattttga
     2341 atggaccaat aaaaacaaca tcacgttgtc aaataatcag accagtttca ccagtggtta
     2401 ttcagttacg gtgacacctg cggccagtaa tgcaaaagtg aatgtttctg cggggggcgg
     2461 cggttcagtg atgattaatg gtgttgcgac attatccagt gcttcatcat cgacacgcgg
     2521 gagtgccgca gtacaatttc tactgtgttt attaggtggc aagtcatggg atgcatgtgt
     2581 aaatagctac agaaatgcat tggcacaaaa tgcaggtgtc tattccttta atctgacatt
     2641 gtcatacaac ccgataacca caacctgcaa accggacgat ttattaatta ctttagacag
     2701 tattcccgtt tcacaattac cagccacagg taacaaagca acaataaata gtaaacaagg
     2761 ggatattatt ctgcgttgta aaaatttatt aggtcaacaa aatcaaacat cacggaaaat
     2821 gcaggtgtat ttatcaagtt ctgatttgtt agccggcagt aacacgatcc tgaaaggtgc
     2881 agaaaataat ggagtcggat ttatgcttga aagcaatggt tctccagtca catttttaaa
     2941 tatcactgat aacagcaaag gatacacaaa tttaaaagaa attgcggcaa agtcaaaact
     3001 tacagataca acggtttcaa ttccgataac tgccagttac tacgtctacg atacaaacaa
     3061 agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg actaataaca
     3121 agcaagacga gcaagtggct aataataaaa aataacaagg tgtctgcatt tccccctcat
     3181 gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt
     3241 taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg
     3301 catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca tcggtgcggt
     3361 acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg aaactactta
     3421 actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg
     3481 cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg gtcataaact
     3541 ctccactttg gggatctgta atatcagtcg catatctttc ctgccataca atataactga
     3601 caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgccc
     3661 taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga atcatcctgg
     3721 gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca tgaagccatg
     3781 tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg gggcgcgttt
     3841 agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga ttgctgcgcc
     3901 aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga ccagtgttgc
     3961 gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg ctgccgggca
     4021 ggggataagg ccaccggtta agccaaatag caaaatttgc cagttggtga cctctctacc
     4081 atcaaagcgt cgtttaatgt cattggcatg ggctcgtgca tgggcatcct gatactcgcc
     4141 atgttcgtga tggtgatgat gtccatggtc gtggtgatct tcgtgatcgt gatgatgatg
     4201 ctcataatca tactcgtgca tattctccag ccagttgcgt tcgccgcgcc aggtacgcca
     4261 gaacatccag aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc
     4321 tgctgattga gcagtaaagc gcttgctgat caccatcccg ccaaaggcaa ttaaccagac
     4381 cactgcggta tgcgaaatag ttgctgccag tccgagcatc accgcttgtt taatggtgcc
     4441 tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg
     4501 aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag
     4561 agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct gattctactc
     4621 cccccagtac ctgatactac cccccagtag attaatagtg ctatgatatt ttcatattct
     4681 tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact gaaagcgcgt
     4741 gccagtaaga ttcagggcta ggtcgtggcg ctcaagaaaa tgctcgacga gccgcatgaa
     4801 tgcgctgcag ttttacaaca gattactgct atccgtggcg cggtaaacgg tctgatgcgg
     4861 gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga gctaaaacgt
     4921 gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata atttattaac
     4981 gcgattgtaa aactgccgtt tttcctcgtt tacaacgcgt gcgctggaca ttaccatcct
     5041 cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag
     5101 aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg accaccaggt
     5161 cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct gggttcagcg
     5221 caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca ctgcatgacc
     5281 aatgatgtgg tgcaaacctt taccgctaat accttgctgg cgctcggtgc atcgccagcg
     5341 atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc cttgttgatt
     5401 aacgttggca cactgacgca gccacgcgct caggcgatgc gtgctgccgt tgagcaagca
     5461 aaaagctctc aaacaccctg gacgcttgat ccagtagcgg ttggtgcgct cgattatcgc
     5521 cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg taatgcttcg
     5581 gaaatcatgg cattagctgg cattgctaat ggtggacggg gagtggatac cactgacgcc
     5641 gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg
     5701 gtcactggcg aggtggatta tgttaccgat ggccatcgcg ccgttggcat tcacggtggc
     5761 gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc
     5821 tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa
     5881 caagccggag agcgcgcagt cgccagaagc gaggggctag gcagttttgt tccacatttc
     5941 cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt aacgctctga
     6001 cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt aaaaccttct
     6061 cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa aatacccgtg
     6121 gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc gattcggtgt
     6181 tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc gatattgttg
     6241 aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc gacaccgtta
     6301 tgctggcaaa aagcggcgac ccactgcttt caccttcggc agttgccacg ctacgcagtc
     6361 gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagcagcc gccttgctcg
     6421 acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg ctgttggcga
     6481 tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa agcccggact
     6541 ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg accaaaaaca
     6601 cccacggtac tggttgtaca ctctctgcgg cgttggctgc actacgcccg cgccatacaa
     6661 actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta gcccaggccg
     6721 acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc tggtggtgag
     6781 caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc agggatgaaa
     6841 ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg cgctcttggg
     6901 ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta ttcgacagca
     6961 gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc tttcgctatt
     7021 cgtattcctg ccagttacac cattcacgga atagatgttt cgcgctggca ggagcggatc
     7081 gactggcagc gtgtggcaaa aatgcgcgat aacggtattc gcttacaatt tgcctttatt
     7141 aaggcgacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg gcaactaagc
     7201 cgcgaaaatg gcttgctgcg cggggcatat cattatttct ccccgtcggt agcagcaccg
     7261 gttcaggtgc gattatttct gcaaacggta gatttctcac aaggcgattt ccctgcggta
     7321 ctggacgtag aagagcgagg aaaattatcg gcaaaagaat tacgcaagag ggtaagtcag
     7381 tggctaaaaa tggtcgaaaa aagtacgggg aaaaagccga ttatttactc aggagccgtt
     7441 ttttatcaca cgaatctggc gggctatttc aatgaatatc cgtggtgggt ggctcactac
     7501 tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca ttccgaccgt
     7561 ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg cacggtggag
     7621 gagttgcagg gattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata
     7681 ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt
     7741 gcccaactga acgaacgtat tttagcggcg gataacacgc cgctttatat taagtttgcc
     7801 gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt
     7861 gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag
     7921 gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac
     7981 atcttcgaat actcgttgaa agaagcgcga ggtttttctc agcaggtggt gttgcgcggg
     8041 aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga aatgtcccga agaagtcgcg
     8101 cagcagctgg cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat tcgctatgtc
     8161 gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt
     8221 gatgccatcg ggatttcact ttatgactat ttccgcagcc agcacattta cccacaacgt
     8281 acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta
     8341 gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct
     8401 attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag
     8461 ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc
     8521 attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc
     8581 acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat
     8641 gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa
     8701 cggcgcggca aatttctcct gccattgctc gccaagtgtt gttatctctg cggataaagc
     8761 aaaacgttcg gcggcaatct cagcctcttg acgattgagc gacactaaag gtcgacaggc
     8821 catgatccgc gccagtaatg catccgggat atcgccaata cgtgggccaa aatcgataaa
     8881 cggcgtcacg tcttgcagct cttccagcca ttccactaac aattcgccgc agggcgaggc
     8941 cagttgataa ccggaaaaat agagcaggct gccaggcgca acggttaatc gcgccagcca
     9001 ctggcgattc cactgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg
     9061 ctcaaccagc gccagacacc agccgttatc accttcggcg ttatcgatca ggctgattaa
     9121 gccctctttt gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa
     9181 ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc
     9241 gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata
     9301 agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt
     9361 taattcaggc agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga
     9421 tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagttcc
     9481 gcctttaacg caggatcgat agcgttaacg ccatgcaacg cgccgcaaat tgccgtcgcc
     9541 atagcaccga tggtgtctgt gtcgccgcca aggttagcgc acaggacggc gcagcgattc
     9601 gggtcggttt gtgctagttc aaccagcgca atggcgcacg gaacggactc aatagtgctg
     9661 gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcattg
     9721 cgcacaattt tcagcgcaat ctccagccgc gccgccagtg aggcgctgaa cgtagtgatg
     9781 cgtttttgct gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc tgaccagctt
     9841 tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga
     9901 tcggatttat gggtcgggct ggacgccagc gctacatcat caataaagga atcaacatca
     9961 cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggtcacgccg
    10021 ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag
    10081 gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc gagaatatta
    10141 cgcccaatca gatccggatc gatcttgcct tcacgttcca gtaacgcatc cgccagacac
    10201 agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc cgcgttattc
    10261 tcctttggtc caggaagaaa acggtcaatc cagccaaagt gtgctttaac gcggctgcgt
    10321 ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata aagagcaccg
    10381 agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg cgatcatcga
    10441 ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac agcacggcga
    10501 taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa gtcagtccgt
    10561 ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga cgatagccga
    10621 gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg gtacgcatat
    10681 gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa tcgtaactta
    10741 cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaatattca tccgtactac
    10801 cgtaaataaa gaagccatag cggatcgcag cggtgaccag accaagcaat aatacctttt
    10861 tgataccaaa gcgtttagtg aaaaacggca atgccagcat aaagaagatt tcagagaact
    10921 ggccgagcgt catccagccg gtggcgtttt tcatgccaac ttcggtcaga taaccgttgg
    10981 caaagatgta atagaacgcc agtggcatcg caaacaggaa tgaacagaag aaaaagacga
    11041 ggaagttttt atcgcgcagc aggatcagcg catccaggcc gagcatgact ttaatatcca
    11101 ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa cgcaaacaca ccgagcagag
    11161 cagaacttcc ggcggtaatc agcagcggga tgttagtcgg tgagatatcg gcatacccca
    11221 gtatttgcgg caagaaacca catgccagac cggaggcaat ccagccgata gtgcccatca
    11281 cacgaatgcg cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg atgctgttag
    11341 tcagcgcaat ggtcggcata taggttagag agtaagccag cagtaacgga aagaacccgg
    11401 caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac atcaataccg
    11461 ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga attggcgaca
    11521 ggatcgccgc aatggcggta caggcatacg accagccaat ttctccggca ctaaaaccgc
    11581 ttttacttaa ccacaaccac aatggcacaa accacgcgcc ccagataaac cattcaacaa
    11641 acatcatgaa cgacagcttt gctgttgttt tcatctttta attccttcat gacaggtaat
    11701 gtaaggtacg cgatgacaat accacttaac aatacctttt aaataccttt gcggtgaaaa
    11761 cttgaccgcg ctaacatttt ttctgatgaa tcgagccaac agaaaacgct gaaaaaacat
    11821 ccaaaagatg gaaaaactcg cccgtaacga ccaggattat gattgcccgc aaaaggcggg
    11881 ccaggacaat ccatagccga tatccaatcg gaatttacgg gagcatagta atgacagata
    11941 ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt atgacaattc
    12001 cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg attgacaata
    12061 atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg cgtctggctg
    12121 gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct gccggagctt
    12181 catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg gcgatcgaag
    12241 cgggctgtaa ctgtgtggcg tcaacttacg gcgtgttggc gtcggtatcg cggcgctatg
    12301 cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt tacccgaata
    12361 cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc gcggtggcgg
    12421 ttggtacgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa gaaatttctg
    12481 cggcctttga acgcgcgcac gagctgggca tggtgaccgt gctgtgggcc tatttgcgaa
    12541 actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg accggtcagg
    12601 caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg gcggaaaata
    12661 acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttat agcaaattga
    12721 ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat atgggccggg
    12781 ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagt gatgcagtgc
    12841 gtactgcggt tatcaacaaa cgcgctggcg gaatggggct gattcttgga cgtaaagcgt
    12901 tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac gtttatctcg
    12961 atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc gtaggacgga
    13021 taaggcgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag cgtcttatca
    13081 tgcctacata gcactgccac gtatgtcgga aaaggcgttt acaccgcatc cggcataaaa
    13141 acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc
    13201 gaacagtttt catattgtcg ttttgtgatc gttatctcga catttaaaaa caaatcattt
    13261 cattatattt tgaaatcgaa aacaagcaac aggatatgaa aatgtacgtg gtatcgacaa
    13321 agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc aatattcaca
    13381 atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca
    13441 tcgccggaac gcctggcaca ttcacttatg ctggtacaga aaatctgttg gcgctggtca
    13501 gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat cacacgaaat
    13561 ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc
    13621 atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt tgccatcgct
    13681 ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat gatgtgcaag
    13741 tcaatgaagc cgatgttttt tataccaacc ccgctcaggc gcgtgaattt gccgaggcaa
    13801 ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat gccagcgcac
    13861 cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc
    13921 tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa ctggggatat
    13981 gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta aaaaattacc
    14041 tgaccgagca ccctgaagcg accgatcccc gggattattt gcagtcggct aaatccgcaa
    14101 tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg gcataacgca
    14161 cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa agctggtgaa
    14221 catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc ggcgctggca
    14281 tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa
    14341 tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt tacgattgcc
    14401 gataaagtcg gatttgcacg cgaacgcatt attctcggcg gcgatcatct ggggccaaac
    14461 tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca
    14521 tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg cgcgggggat
    14581 cccataccgt tagcaccaga aacggttgcg gaacgagctg ctttgctttg ctgcggaaag
    14641 tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg aagttccggt
    14701 tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg aagatgccgc
    14761 caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag aggcgttaac
    14821 acgcgtgatt gccatcgtgg tgcagccggg cgtggaattt gatcacagca atattatcca
    14881 ttatcagccg caggaagcgc agccgctggc gcaatggata gaaaacaccc gaatggttta
    14941 tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag tccgcgatca
    15001 ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg cgatatttgc
    15061 actggcacaa attgagcagg aacttatcgc ccctgaaaat cgcagcggtt gcctggcggt
    15121 aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc gtacgggttt
    15181 taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt attactggcc
    15241 gcatagtcgg attaaaaata gcgtcgaaac gatgatggtg aatcttgaag gcgtggacat
    15301 cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc agtccgggga
    15361 attatcagca ataccgcatc agctgattat ggataaaatt tatgatgttt tgcgcgccta
    15421 tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc gtagcggaat
    15481 ttcttttgtc gatcgtagcg aagttttaac ccatatcggt aatgagatgc tcgccaaagg
    15541 tgtggttcat gatacctggc cacaggcatt aattgccaga ga
//
LOCUS       DAZPJR010000091        15535 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPJR010000091 DAZPJR010000000
VERSION     DAZPJR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15535
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.092"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1137
                     /locus_tag="ACHVB7_002672"
     CDS             <1..1137
                     /locus_tag="ACHVB7_002672"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1914616.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGG
                     FLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYL
                     GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYE
                     FICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMR
                     IMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1293..2828
                     /gene="gadC"
                     /locus_tag="ACHVB7_002673"
     CDS             1293..2828
                     /gene="gadC"
                     /locus_tag="ACHVB7_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR1914617.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2959..4278
                     /locus_tag="ACHVB7_002674"
     CDS             2959..4278
                     /locus_tag="ACHVB7_002674"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR1914618.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4650..6032
                     /gene="dosC"
                     /locus_tag="ACHVB7_002675"
     CDS             4650..6032
                     /gene="dosC"
                     /locus_tag="ACHVB7_002675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR1914619.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            6057..8456
                     /gene="dosP"
                     /locus_tag="ACHVB7_002676"
                     /gene_synonym="pdeO"
     CDS             6057..8456
                     /gene="dosP"
                     /locus_tag="ACHVB7_002676"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR1914620.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8715..9296
                     /gene="ddpX"
                     /locus_tag="ACHVB7_002677"
     CDS             8715..9296
                     /gene="ddpX"
                     /locus_tag="ACHVB7_002677"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR1914621.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            9310..10860
                     /locus_tag="ACHVB7_002678"
     CDS             9310..10860
                     /locus_tag="ACHVB7_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1914622.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10862..11884
                     /locus_tag="ACHVB7_002679"
     CDS             10862..11884
                     /locus_tag="ACHVB7_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1914623.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11881..12777
                     /gene="ddpC"
                     /locus_tag="ACHVB7_002680"
     CDS             11881..12777
                     /gene="ddpC"
                     /locus_tag="ACHVB7_002680"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR1914624.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12774..13760
                     /locus_tag="ACHVB7_002681"
     CDS             12774..13760
                     /locus_tag="ACHVB7_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1914625.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            13789..14679
                     /locus_tag="ACHVB7_002682"
     CDS             13789..14679
                     /locus_tag="ACHVB7_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR1914626.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(14735..15166)
                     /gene="osmC"
                     /locus_tag="ACHVB7_002683"
     CDS             complement(14735..15166)
                     /gene="osmC"
                     /locus_tag="ACHVB7_002683"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR1914627.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3816 a         3875 c         3952 g         3892 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgc gacgaaaata ccatcggcgt ggtgccgact
      361 ttcggcgtga cctacaccgg taactatgag ttcccgcaac cgctgcacga tgcactggat
      421 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
      481 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      541 tcgatcagtg cttcaggcca caaattcggt ctggctccgc tgggctgcgg ctgggttatc
      601 tggcgtgacg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggtggt
      661 cagattggta cttttgccat caacttctcc cgcccggcgg gtcaggtgat tgcacagtac
      721 tatgaattcc tgcgccttgg tcgtgaaggc tataccaaag tacagaatgc ttcctaccag
      781 gtcgctgcct atctggcgga tgagatcgcc aaactggggc cgtatgagtt catctgtgcc
      841 ggtcgcccgg acgaaggcat cccggcggtt tgcttcaaac tgaaagaggg tgaagatccg
      901 ggatacaccc tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc
      961 ttcactctcg gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc
     1021 ttcgaaatgg actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc
     1081 agcgatcacc cgaaactgca gggtattgcc cagcagaaca gctttaaaca tacctgataa
     1141 cgtttaacgg taacggtgtc ccgaaacgaa cccgtttcgg gataatttcc aaagtctgtt
     1201 cactggcatt agcaacggaa aatattgttc tgaatacgct tcagaacaaa acaggtgcgg
     1261 ttccgacagg aataccgttt tagggggata atatggctac atcagtacag acaggtaaag
     1321 ctaagcagct cacattactc ggcttttttg ccataacggc atcgatggta atggctgttt
     1381 atgaataccc taccttcgca acatcgggct tttcattagt cttcttcctg ctattaggcg
     1441 ggattttatg gtttattccc gtgggacttt gtgctgcgga aatggccacc gtcgacggct
     1501 gggaagaagg tggtgtcttc gcctgggtat caaatactct ggggccgaga tggggatttg
     1561 cagcgatctc atttggctat ctgcaaatcg ccattggttt tattccgatg ctctatttcg
     1621 tgttaggggc actctcctac atcctgaaat ggccagcgct gaatgaagac cccattacca
     1681 aaactattgc agcactcatc attctttggg cgctggcatt aacgcagttt ggtggcacga
     1741 aatacacggc acgaattgct aaagttggct tcttcgccgg tatcctgtta cctgcattta
     1801 ttttgatcgc attagcggct atttatctgc actccggtgc ccccgttgct atcgaaatgg
     1861 attcaaagac cttcttccct gacttctcta aagtgggcac cctggttgta tttgttgcct
     1921 tcattttgag ttatatgggc gtagaagcat ccgcaaccca cgtcaatgaa atgagcaacc
     1981 cagggcgcga ctatccgttg gctatgttac tgctgatggt agcggcaatc tgcttaagct
     2041 ctgttggtgg tttgtctatt gcgatggtca ttccgggtaa tgaaatcaac ctctccgcag
     2101 gggtaatgca aacctttacc gttctgatgt cccatgtggc accggaaatt gagtggacgg
     2161 ttcgcgtgat ctccgcactg ctgttgctgg gtgttctggc ggaaatcgcc tcctggattg
     2221 ttggtccttc tcgcgggatg tatgtcacag cgcagaaaaa cctgctgcca gcggcattcg
     2281 ctaaaatgaa caaaaatggc gtaccggtaa cgctggtcat ttcacagctg gtgattactt
     2341 ctatcgcgtt gatcatcctc accaataccg gtggcggtaa caacatgtcc ttcctgatcg
     2401 cactggcgct gacggtggtg atttatctgt gtgcttattt catgctgttt attggctaca
     2461 ttgtgttggt tcttaaacat cctgacttaa aacgcacatt taatatccct ggtggtaaag
     2521 gggtgaaact ggtcgtggct attgtcggtc tgctgacttc aattatggcg tttattgttt
     2581 ccttcctgcc gccggataac atccagggtg actctaccga tatgtatgtt gaattactgg
     2641 tcgttagttt cctggtggta cttgccctgc cctttattct ctatgctgtt catgatcgta
     2701 aaggcaaagc gaataccggc gtcactctgg agccaatcaa cagtcagaac gcaccaaaag
     2761 gtcacttctt cctgcacccg cgtgcacgtt caccacacta tattgtgatg aatgacaaga
     2821 aacactaacc atgtctgaat ggttataaga caagggagcg ataattcatc gctccctttt
     2881 tcgtgctcgc tgtcgtattg acttaaccgg gataagtacg agaatgagcg cacatctgtt
     2941 taccggaaac cagcatatat ggatatctgc tcccgaaacg agaaattagc gattagaaga
     3001 ccagcgatac tggttgcact ggcactttta ctgtgtagtt gtaaaagcac gcctccagag
     3061 tccatggtga caccaccagc aggttcaaag ccaccagcca cgacgcaaca atcgtcacaa
     3121 ccgatgcgtg gcatctggct ggccacggtg tctcgcctcg actggccacc ggtttcctcg
     3181 gttaacatta gtaaccccac cagccgggcc cgtgtacaac aacaggcgat gatcgacaaa
     3241 ctggatcatc tgcaacgtct cggtataaac acggtctttt tccaggttaa gccggacggt
     3301 accgccctgt ggccatcgaa aattttgcca tggtccgatc ttatgaccgg taagattggt
     3361 gaaaatccgg gttacgatcc gctgcaattt atgctcgacg aagcccacaa gcgtgggatg
     3421 aaagtacacg cctggtttaa cccctatcgc gtatcggtta atacgaagcc cggtactatc
     3481 agggaactga atagcactct gtctcaacaa ccggcgagcg tctatgtgca acaccgtgac
     3541 tggatcagaa cgtctggcga tcgctttgtc ctcgacccgg gcatccctga ggttcaggac
     3601 tggatcacat caatagtcgc agaagtggtt tcccgctatc cggtagatgg cgtgcagttt
     3661 gacgactatt tctatactga gtcaccgggt tcacggctaa atgataacga aacgtaccgc
     3721 aaatacggag gcgcatttgc gtcaaaagca gactggcggc gcaacaatac tcagcagtta
     3781 attgcaaaag tatcgcacac cattaaaagc attaagccgg gagtcgaatt tggcgttagc
     3841 ccggcaggcg tgtggcgtaa ccgatcacac gatccgctcg gttccgatac ccgaggcgcg
     3901 gcagcctatg acgaatccta cgctgacacc cgtcgatggg tggaacaagg attgctggat
     3961 tacattgctc cccaaattta ctggccgttc tcacggagtg ccgcgcgtta tgacgtgttg
     4021 gcaaaatggt gggcggatgt cgttaaaccg actaggaccc gcctgtatat cggtatcgcc
     4081 ttctataaag tgggtgaacc ttcaaagata gagccagact ggatgattaa cggcggcgta
     4141 ccggaactga aaaagcagct cgatcttaac gatgcggtac cagaaattag cggcaccatc
     4201 ttgttccgtg aggactatct gaataaaccg cagactcaac aagcggtcag ctatctgcaa
     4261 agtcgttggg gcagttaaac tcccatgctt actttgtaac aagccggtgc tttacccacc
     4321 ggcttgttac accttgtgaa atatcccact agtaatcatg cttacataag tcaaattaac
     4381 cacgcaatta accacactta gttaaacagc tataaatccc taataaaaat gtatgaatat
     4441 gaaaaaccat acgatattcg ctgtcaccga aaaataatta cgatgggcta tactcttctg
     4501 aagaacagta gcgtagattg aaactgtcct aagtcgctta tttcttttca gtatatcttc
     4561 atatttcagg agaatatggt gcagttagtc aatctcgcac gttgcgtttt gattccgtct
     4621 gatttcccag ccttataagg gtgtgaatta tggagatgta ttttaaaaga atgaaagatg
     4681 agtggaccgg acttgtcgaa caggcagatc cactcattcg tgctaaagcc gcggaaattg
     4741 ccgttgcgca tgctcattat ctgagtatcg agttttatcg aattgtccgc atcgacccgc
     4801 atgccgaaga attcttgagt aatgaacaag ttgagcggca gttgaaaagt gcgatggaac
     4861 gctggattat taacgtgctt tctgcccagg ttgacgatgt cgaaaggcta atacaaatcc
     4921 agcataccgt cgcggaagtg catgcccgca taggaattcc ggtagaaatt gtcgagatgg
     4981 ggtttcgggt gctgaaaaaa atcctctatc cggtaatctt ctcttcggat tattccgccg
     5041 cagaaaaact tcaggtctat catttctcga ttaacagtat tgatatcgcg atggaagtga
     5101 tgacccgcgc gtttaccttt agtgacagta gtgcctcaaa ggaagatgaa aattatcgta
     5161 tcttctcgtt actggaaaac gccgaagaag aaaaagaacg gcaaatagcc tcaatacttt
     5221 catgggaaat agatattatc tataaaatcc tgctggattc tgatttaggc agtagtttgc
     5281 cattaagcca ggctgatttt ggcctgtggt ttaaccataa aggtcgacat tattttagtg
     5341 gcattgccga agtaggccat atctcccgtc tcattcagga tttcgacggt attttcaatc
     5401 aaaccatgcg taacaccagg aatttgaata acagaagtct gcgggtgaaa tttttattac
     5461 agataagaaa taccgtatcg cagattatta ccttgctgcg tgaattgttt gaagaagtat
     5521 cgcgccacga agtcggtatg gatgtactaa cgaaattact taaccgccgt ttcctgccga
     5581 ctatcttcaa acgcgaaatt gcccatgcca accggaccgg tacaccgctg tcagtgctga
     5641 ttattgacgt tgataaattc aaagagatca acgatacgtg gggccataac actggcgatg
     5701 aaattctgcg taaagtctct caggcctttt atgacaacgt ccacagtagt gattatgttt
     5761 ttcgctacgg gggcgatgaa tttatcattg ttttgactga agcttctgaa aacgaaacgt
     5821 tacgtaccgc agaacgtatt cgcagtcggg tggagaaaac caaactgaaa gccgcaaacg
     5881 gcgaggatat tgccctctca ctttccatcg gtgccgccat gtttaatggt catcctgact
     5941 atgagcgcct cattcaaata gccgatgaag ctctgtatat cgccaaaaga cgaggtagaa
     6001 accgtgttga actctggaaa gccagtcttt agatgcgcca ggatgcagag gtaatcatga
     6061 agctaaccga tgcggaaaat gccgccgatg gcattttttt ccccgccctt gaacaaaata
     6121 tgatgggcgc ggtgttaatt aacgaaaatg atgaagtgat gtttttcaac cccgccgcag
     6181 agaagctctg ggggtacaaa cgtgaagaag tcattggcaa taacattgat atgctgattc
     6241 cacgggattt gcgtcctgcg catcctgaat acattcgtca taaccgtgaa ggtggtaaag
     6301 cgcgcgtcga ggggatgagt cgggagctgc agctggagaa aaaagacggc agtaaaatct
     6361 ggacccgttt tgcgctatcg aaagtgagcg ccgaggggaa agtttattac ctggcgctgg
     6421 tacgggatgc cagcgtagaa atggcgcaaa aagaacagac ccgacaattg attattgccg
     6481 ttgaccatct cgaccgaccg gtgattgtcc tcgatccgga acgccatatt gtgcagtgca
     6541 atcgcgcatt taccgaaatg tttggttact gcattagcga agccagcggt atgcagcccg
     6601 atacactcct gaacattcct gaattccctg ccgataaccg cattcgttta caacagttgc
     6661 tatggaaaac cgcccgcgat caggacgaat ttctgctgtt gacacgcacc ggtgaaaaaa
     6721 tctggattaa agcctctatc agcccggttt atgacgtgct cgcgcatctg cagaacctgg
     6781 taatgacctt ctcggatatc accgaagaac ggcagatccg ccagcttgaa ggcaatattc
     6841 tcgccgccat gtgcagcagc ccgccatttc atgaaatggg ggaaatcatt tgtcgtaaca
     6901 tcgaatctgt tctcaacgaa tcgcatgttt cgctgttcgc actgcgcaac gggatgccga
     6961 tacactgggc gtcatcttcg cacggtgcag aagttcaaaa tgcgcaaagc tggtcagcga
     7021 ccattcgtca gcgtgatggt gcgcctgcgg ggatcctgca aattaaaacc tcgtcaggag
     7081 cagaaaccag cgcctttatc gaacgcgtgg cagatatcag ccagcatatg gccgcgctgg
     7141 cgctggaaca ggaaaaaagc cgtcagcata ttgaacaact catccaattt gatccgatga
     7201 ccggtctgcc aaatcgcaat aacctgcaca attacctcga tgaccttgtc gacaaagccg
     7261 tctctccggt ggtgtatctc atcggtgttg accatattca ggatgtgatt gatagccttg
     7321 gctatgcgtg ggccgatcag gcattgctgg aagtggtcaa tcgctttcgt gaaaaactca
     7381 aaccggatca gtatctctgt cgtatcgaag gtacgcagtt tgtcctcgtg agcctggaaa
     7441 acgacgtcag taacattacc caaatcgccg atgagctacg gaatgtggtc agcaagccga
     7501 taatgattga cgataaacca ttcccgctaa ccttgagtat tggcatcagc tacgacgtgg
     7561 gtaaaaaccg cgattacctg ctctccactg ctcacaatgc aatggattat attcgcaaga
     7621 atggcggtaa cggctggcag ttcttcagcc cggcgatgaa cgaaatggta aaagagcgtt
     7681 tggttttagg cgcagcgctg aaagaagcga ttagcaataa ccaactgaaa ctggtttacc
     7741 agccgcaaat cttcgcagaa acgggtgaac tgtacggcat cgaagccctt gctcgctggc
     7801 acgatcccca gcatggtcat gtaccccctt cacggtttat tcctctcgca gaagagattg
     7861 gtgaaatcga aaatattggg cgctgggtca tcgcggaagc ttgccgtcag ttagcagaat
     7921 ggcgtagcca gaatattcat atcccggcgt tatccgtgaa cttgtcggcg ctgcactttc
     7981 gcagtaatca gctgcctaat caggtgtctg atgcaatgca agcctggggt attgacggcc
     8041 accagctgac ggtagaaatc acggaaagca tgatgatgga acacgatacc gaaatcttta
     8101 agcgcattca gatcctgcgc gatatgggcg tgggcttatc ggtagatgat tttggcacgg
     8161 gcttttccgg attatcccgc ttagtcagtc ttccggtaac ggaaatcaaa attgacaaaa
     8221 gttttgtcga tcgttgtctg acggaaaaac gcatccttgc cttacttgaa gccattacca
     8281 gcattgggca aagcctcaat ttaaccgtcg tggcggaagg cgtcgaaacc aaagagcaat
     8341 ttgagatgct acgcaagatc cactgtcgcg ttattcaggg atatttcttt tcccgccccc
     8401 tacccgccga ggaaattcca ggctggatga gcagcgtgtt accgctgaaa atctgacaaa
     8461 ttcctctcgc ccgcactcgc gggttttcat ttaacgtgac gctgtcactt aaatactgtg
     8521 atttcagcta cgattccggg agattccttc cttaacttcc cctcttgcac caaaactgca
     8581 ctacgctgca caaatgttga tcaaaagtta aaaaatataa ctttaagcca gcattctggc
     8641 gcttatcccg gcatggcatg agatctgcat aagcggaaag cgcagcaatt tttgtcttaa
     8701 tacaggaacc gtttatgtcg gataccaccg aactggttga tttagccgtg atcttccctg
     8761 atctggagat cgaattgaaa tacgcctgcg ctgataacat cacaggtaaa gctatttacc
     8821 agcaagcgcg ttgtctgtta cacaaggatg cgattaccgc gctggcgaaa agtatcagta
     8881 tcgcccagct gtcaggctta caactggtga tttacgatgc gtatcgccca caacaagcac
     8941 aggcgatgtt gtggcaagcc tgcccagacc cgcaatatgt tgttgatgtg acggtcggtt
     9001 ctaatcacag ccgtggcacg gcgatcgacc tgacgcttcg tgatgagcac gggaacatcc
     9061 tggatatggg cgcagggttc gatgaaatgc acgagcgctc ccatgcctat cacccttccg
     9121 tcccgcccgc cgctcagcgc aatcgcctgt tgctgaatgc gataatgact ggtggcggtt
     9181 ttgtcggtat ctccagcgaa tggtggcact tcgaattacc ccaggcagcg agttaccctc
     9241 tgctcgctga tcaattctct tgttttatat cccctggcac acagcacgtc agttaatcag
     9301 gaaactgtca tgaagagatc gatatcgttt cgtcccacat tgctcgcgct cgtccttgcc
     9361 acaactttcc cggttgcgca cgccgccgta ccgaaagata tgctggtgat tggtaaggcc
     9421 gccgatccac aaaccctcga cccggcggta acaatagata ataacgactg gacagtgacc
     9481 tacccgtctt atcagcggct ggttcagtac aaaacggacg gtgataaagg ctcaaccgac
     9541 gttgaaggcg atctggcaag tagctggaaa gcgtctgacg atcaaaaaga gtggacgttc
     9601 accctgaagg acaatgctaa atttgccgat ggcacacctg tcactgccga agcagtaaaa
     9661 ctctcttttg agcggttact aaaaatcggc caggggccag cagaagcatt tcccaaagat
     9721 ttaaagattg atactcccga cgaacatacg gtgaagttta cccttagcca accattcgca
     9781 ccgttcctct acacgctagc gaatgacggt gcatccatta tcaatccggc ggtcttaaag
     9841 gaacatgcag cggatgatgc tcgcggcttc ctcgcgcaaa ataccgccgg ttccggacca
     9901 tttatgctga aaagcgggca aaaaggtcag caattagttc tggtgccaaa tccgcattac
     9961 cccggcaata aaccgaactt taagcgagta tcggtaaaaa ttatcggtga aagtgcctcc
    10021 cgtcgcctgc agctctcccg tggcgacatt gacattgccg atgcgctgcc ggtggatcaa
    10081 ctcaacgccc tgaagcagga aaataaagtc aatgtggcag agtatccgtc actgcgcgtt
    10141 acctatctgt atctgaataa cagcaaagcg cctcttaatc aggcggatct gcgtcgggcc
    10201 atttcctggt ctaccgatta tcagggcatg gttaacggca ttctgagtgg taacggaaaa
    10261 cagatgcgcg gcccgattcc ggaaggcatg tggggctacg atgcgacggc aatgcaatac
    10321 aaccatgacg aaacgaaagc caaagccgaa tgggataaag tgacgagcaa acccaccagc
    10381 ctgacgtttc tctactccga taacgatccg aactgggagc ctattgctct ggcgacacaa
    10441 tccagtctca acaagctggg catcaatgtg aagctggaaa agctggcgaa cgccaccatg
    10501 cgcgacagag tgggtaaagg tgattacgac attgcgattg gcaactggag tccggatttt
    10561 gccgacccgt atatgtttat gaattactgg tttgagtcag acaaaaaagg tctgccgggt
    10621 aaccgctcgt tctatgaaaa cagtgaggtc gataagttac tgcgcaatgc gcttgcgacc
    10681 accgaccaga cgcagcgtac ccgggactac cagcaggcac agaaaatcgt cattgatgac
    10741 gctgcttatg tgtacctgtt ccagaaaaac taccaactgg cgatgaacaa agaggtgaaa
    10801 ggctttgtgt tcaatcccat gctggaacag gtcttcaata tcaataccat gagtaaataa
    10861 cgtgaccttc tggagtattt tacgccaacg ctgctggggg ctggtgctcg tggtggcggg
    10921 cgtctgcgtg attacgttta ttatctcgca tctgatccct ggcgatccgg cgcggttact
    10981 ggcgggtgac cgcgccagcg atgctatcgt ggaaaatatt cgccagcaac tgggactgga
    11041 ccagccactg tatgtacagt tttaccgcta cttcagcgac ctgtttcatg gtgacctggg
    11101 aacatccatt cgtaccgggc gtccggtgct ggaagagttg cgtatatttt tcccggcgac
    11161 gctggaactg gctttttgtg ccctgctgct ggcactcctg attggcatcc cgctgggcat
    11221 actctctgca gtctggcgaa atcgctggct ggatcatctg gtgcgaataa tggccattac
    11281 cggaatctcc acacctgcgt tctggcttgg actgggcgtc attgtgctgt tttatggtca
    11341 tctgcaaatt cttcccggcg gcggaaggct tgatgactgg ctggatccac caacgcacgt
    11401 taccggcttt tatctgctcg atgcgctgct tgaaggcaac ggtgaagtct tcttcaatgc
    11461 gttgcaacat ctcatcttac cggcattaac gctggcgttc gttcacctgg gaattgtcgc
    11521 tcgccagatc cgctcagcga tgctggaaca attgagtgaa gactacattc gtaccgcccg
    11581 ggccagcggc ttgcccggct ggtatatcgt tttatgttat gcgctaccca atgcgttgat
    11641 cccatcgatt accgtattgg gtctggcgct gggcgatttg ttgtatggcg cagtgctcac
    11701 cgaaaccgtt tttgcctggc ccggaatggg tgcatgggta gtaacatcaa tacaggcgct
    11761 cgacttcccg gcagtgatgg gctttgccgt cgtggtttca tttgcttatg tgctggtcaa
    11821 cctggtggtg gatttgctct atttgtggat tgatccgcgt atcggacgtg gaggtggtga
    11881 atgatgctaa gcgaggaaac gtccgccgta cgcccacaaa aacaaacgcg atttaacggt
    11941 gcaaaactgg tatggatgct gaaaggcagt ccgctcaccg tgaccggcgc agtcatcatt
    12001 gtattaatgc tattgatgat gattttttca ccgtggcttg cgacgcatga tcccaacgcc
    12061 attgatttaa ccgcccgcct tttgccgcct tctgcggcgc actggtttgg caccgatgaa
    12121 gtgggacgcg atctgttcag ccgcgtactg gtcggcagtc agcaatcaat tctcgccgga
    12181 ttagtcgtgg ttgccattgc gggtatgatt ggttcgctac tcggatgtct atccggtgtg
    12241 cttggcggac gcgcagacgc catcatcatg cgcatcatgg acattatgct gtcgattcct
    12301 tcgttggtac tgacaatggc actggctgcc gctctcgggc cgagtttatt taacgccatg
    12361 ctggcgattg ctattgtgcg aattcccttt tatgtgcgcc tggcgcgggg gcaagcatta
    12421 gttgtacgcc agtataccta tgttcaggcg gcgaaaacct ttggtgcgtc gcgttggcat
    12481 ctgatcagct ggcatatttt acgtaactcc ctgccgccgc tgatcgtgca ggcatcgctg
    12541 gatatcggta gcgcgatttt aatggccgcc acgttgggat ttattggcct gggtgctcaa
    12601 caaccgagtg ctgaatgggg cgcgatggta gctaatggtc gcaactatgt actcgatcag
    12661 tggtggtatt gcgcatttcc gggggcagcg attttgctta ccgccgtcgg gtttaatctc
    12721 tttggtgatg gtattcgcga tctgcttgac ccgaaagcag gaggaaagca gtcatgaccc
    12781 aacccgttct ggacattcaa cagctgcatt tgagtttccc cggttttaac ggttatgttc
    12841 acgtgctcaa caatgtgtcc ttgcagatta accgcggtga aattgtcggt ctggtgggag
    12901 aatccggctc aggtaaatca gtcaccgcaa tgctgattat gcgtctgcta ccgacgggca
    12961 gttattgcgt acatcggggg cagatttcac tgctgggaga cgatgttctt aacgctcggg
    13021 agaaacagct tcgtcagtgg cgcggcgcac gagtggcgat gatctttcag gaaccgatga
    13081 ccgccctcaa tccgacacgt cgaataggtc ttcagatgat ggacgtgatc cgccatcatc
    13141 aaccaataag tcgtcgggaa gccagagcta aagcgattac cctgctggaa gagatgcaaa
    13201 tcccggatgc cgtggaagtt atgtcgcgct atccgtttga gctttcaggt ggtatgcgcc
    13261 agcgggtaat gattgcgctg gcattctcct gcgagccgca attgattatt gccgacgaac
    13321 cgactacggc gctggacgtc acggtacagt tgcaggtact gcgtttgctt aaacataaag
    13381 ctcgcgccag tggaactgcg gtactgttca tcagccatga tatggccgtg gtgtcgcaac
    13441 tgtgcgatag cgtttacgtg atgtatgccg gaagcgtaat tgaaagcggc gtgacggcag
    13501 acgttatcca tcatccccgg catccgtata ccattggttt gctgcaatgc gcaccagaac
    13561 atggagtacc acgccagcca ttacccgcca ttccagggac ggtaccaaac ctcacccatt
    13621 tgcctgacgg ctgcgctttt cgcgatcgtt gctatgcggc aggtgcacag tgtgaaaacg
    13681 tcccggcgct gacagcgtgt ggtgacaaca accagcgctg cgcctgttgg tatccgcagc
    13741 aggaggtcat aagtatctga cacgttatta acgttacgcg acgtccatat caatttcccg
    13801 gcccgtaaaa gctggcttgg taaaactacg gaacatgttc atgcgattaa tggtattgat
    13861 ttacagatcc gccgtggtga aaccttaggg atcgtcggtg agtcaggctg cggcaaaagc
    13921 accctcgcac agcttttaat gggtatgctg caaccgagcc acgggcaata catccgttca
    13981 ggatcacaac gcattatgca gatggtgttt caggacccgc tctcttcgct taatccgcgc
    14041 ttatcggtgt ggcgcatcat cacagaaccg ctctggatag ctaagcgtag tagtgaacaa
    14101 cagcggcgag cgttggcaaa ggagttggct gtgcaggtgg gtattcgtcc ggagtatctc
    14161 gaccgcctgc ctcatgcgtt ctccggcggg cagcggcaac gcatcgccat tgccagagca
    14221 ctctcttcgc agcctgacgt gattgtgctt gatgagccaa cctcggcgct ggatatctcc
    14281 gtgcaggcgc agatcctcaa tttactggta acgctacagg aaaatcacgg gctgacttat
    14341 gtgctgattt cacacaatgt ctcggtgata cgtcatatga gcgatcgggt ggcggtgatg
    14401 tatctcgggc agattgtaga actgggcgac gcgcagcagg tgctgacggc acctgcacat
    14461 ccatacaccc gattattgct ggattccctc cccgccattg ataaaccgct ggaggaagaa
    14521 tgggcattat gtaaaacgga tctgccggga aaccgcacgt tgccacaagg ctgttttttc
    14581 tacgaacgtt gccctctggc aacccacgga tgtgaagtcc ggcaatcatt agcaataagg
    14641 gaggacggac gtgagctccg gtgctggcgg gcgctgtaga gatgctaaca acgcatcagg
    14701 cattcacgcg cctgacgcgt catccggcaa tgctttacga tttcaactgg taatccagcg
    14761 taatttccgc tttcagtacc tgagaaaccg ggcatcctgc ttttgctttc tggatgatgc
    14821 cgtcaaaggt agaggcatca atacccggca ccgcaacttc actcttcagt gcgattttcg
    14881 taatcgcaaa atcggcatcc actttatcca gcgacacatc ggcggtggta tcaatcgatg
    14941 ttggcgtgaa tcccgcttcc cccagcatta atgaaagcgc cattgagaaa catgcggcat
    15001 gcgctgcgcc aatcagttct tcagggttgg ttcctttttc gccttcaaaa cgcgtgttaa
    15061 atccatacgg ctgttggttc agcacgccac tctgggtgga tactgttccc ttcccgcgtt
    15121 tgatatcgcc ttcccagtgt gcctgacctt tcttatggat tgtcattgtt gctctcctgt
    15181 gggcttaaaa aggaaattat agactacgtg agaaacagca cgaggataag caggatattc
    15241 cgaataaaat acggcgaaaa tattacttct gcacttaaat taaattccca caatagataa
    15301 ccgggaatat atatctcaag agatattgca tcaatatcaa tcaactcacc acaataataa
    15361 tacatatcaa tatcatggag ttaaaatata aaaatcatca accaggacta atcttaacaa
    15421 cgaagcggca aatatttgcc atgctgaatg tgctttataa atctgcgatc cgtagcagac
    15481 accataaata cacagacacg gagaatcact atgtttactt attatcaggc agaaa
//
LOCUS       DAZPJR010000092        15445 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPJR010000092 DAZPJR010000000
VERSION     DAZPJR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.093"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(98..1210)
                     /gene="ydiK"
                     /locus_tag="ACHVB7_002684"
     CDS             complement(98..1210)
                     /gene="ydiK"
                     /locus_tag="ACHVB7_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR1914628.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1599..4655
                     /locus_tag="ACHVB7_002685"
     CDS             1599..4655
                     /locus_tag="ACHVB7_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR1914629.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4652..5062
                     /gene="menI"
                     /locus_tag="ACHVB7_002686"
     CDS             4652..5062
                     /gene="menI"
                     /locus_tag="ACHVB7_002686"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR1914630.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5162..5350
                     /locus_tag="ACHVB7_002687"
     CDS             5162..5350
                     /locus_tag="ACHVB7_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR1914631.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5898..6266
                     /gene="sufA"
                     /locus_tag="ACHVB7_002688"
     CDS             5898..6266
                     /gene="sufA"
                     /locus_tag="ACHVB7_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR1914632.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6275..7762
                     /gene="sufB"
                     /locus_tag="ACHVB7_002689"
     CDS             6275..7762
                     /gene="sufB"
                     /locus_tag="ACHVB7_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR1914633.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASELGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7772..8518
                     /gene="sufC"
                     /locus_tag="ACHVB7_002690"
     CDS             7772..8518
                     /gene="sufC"
                     /locus_tag="ACHVB7_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR1914634.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8493..9764
                     /gene="sufD"
                     /locus_tag="ACHVB7_002691"
     CDS             8493..9764
                     /gene="sufD"
                     /locus_tag="ACHVB7_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR1914635.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9761..10981
                     /gene="sufS"
                     /locus_tag="ACHVB7_002692"
     CDS             9761..10981
                     /gene="sufS"
                     /locus_tag="ACHVB7_002692"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR1914636.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10994..11410
                     /gene="sufE"
                     /locus_tag="ACHVB7_002693"
     CDS             10994..11410
                     /gene="sufE"
                     /locus_tag="ACHVB7_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR1914637.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11559..12563
                     /gene="ldtE"
                     /locus_tag="ACHVB7_002694"
     CDS             11559..12563
                     /gene="ldtE"
                     /locus_tag="ACHVB7_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR1914638.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12626..12862)
                     /gene="lpp"
                     /locus_tag="ACHVB7_002695"
     CDS             complement(12626..12862)
                     /gene="lpp"
                     /locus_tag="ACHVB7_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR1914639.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13172..14584)
                     /gene="pykF"
                     /locus_tag="ACHVB7_002696"
     CDS             complement(13172..14584)
                     /gene="pykF"
                     /locus_tag="ACHVB7_002696"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR1914640.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(14592..14786)
                     /locus_tag="ACHVB7_002697"
     CDS             complement(14592..14786)
                     /locus_tag="ACHVB7_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914641.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            15141..15350
                     /gene="fumD"
                     /locus_tag="ACHVB7_002698"
     CDS             15141..15350
                     /gene="fumD"
                     /locus_tag="ACHVB7_002698"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR1914642.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3902 a         3736 c         4089 g         3718 t
ORIGIN      
        1 cagctgaatg gcggtaagca ggaaaatatg gggaagagca agcaaatatc aattagcggt
       61 gattaacaat caggcggcag agccgcctga tgagaaatta cttattcggt ttctcgattt
      121 cgccaagttc ttcgagaatt tcttccggtt ggtccgtcgg cggcgggact tcttccaccc
      181 acgcggcaaa cagacgccag gaaacggcta acagaaccgg accaataaac agaccgatca
      241 tcccgaaagc aatcaaacca ccaataacgc cagagaggat caggatcagc ggtaaatcgg
      301 cacccatgcg aattaacatt gggcggatga cgttatccag cgtgccaacc acaccgctcc
      361 acactaacaa taccgttccc caggtggtat cgccggtcca gtagagccag ataatcgccg
      421 gaatcagtac cggcaacggg ccaagctgga caaggcagga gaggatcatt aacaccgtta
      481 gcaacgttgc ataaggtacg ccggatacgg cgaggccgat actgccaagc actgcctgta
      541 ctaacgccgt caccaccaca cccagcgcca ccgcgcggat agcctgtgcc gccagcagca
      601 cggcggcatc tccgcgaacg cctgccagac gggttgcaaa atggcgaatg ccttgtgcca
      661 cctgttcacc gcgccagtac agcagggcac tgaagagaag catcagcgca caatgcacca
      721 taaagcgccc gatatgcgcc gcctgcccaa cgaaccaggt ggtggtggtg ccaatataag
      781 ggcggacttt cgccatgatc gccgtacccc ccatatccag caagttgtgc cagcctgcat
      841 acagcttcgc gccaatcacc ggaatggtat taagccacgc cagatcgggt aacgtcatgt
      901 cgccgctgga aatggcttta attagcgggc cgctgccgtc gacgatactg ttaaccagca
      961 aagcaatagg gatgataaac accatcacta ataacagcgt catcaccaga acggcgagag
     1021 agcggcggcc aaacatgatc ttttgcaaac gtaacaatac cggccaggtg gcgataacca
     1081 ccgtaccggc ccatgcaaag ccgagaataa agggttgaac aatccacaga catgccacaa
     1141 tcatgatggc taaaaacagc accgaaagca gaatttgtgc gacatccctg ggctgacgaa
     1201 catttaccat aactactttt cacctttttt gtgcgtcaaa tcgtcgacgc gacgtgaaca
     1261 cgcgaaactc acctgcataa tgatgagcaa tttcagcggt ttttaacagg ccgattctgc
     1321 ccttaattct gatgggcgtg ttagaaaaaa atgtggtaca acaactcagg caacacgcaa
     1381 acgatttact cgtcgtattt caagttgcat gcgctgcggc tacgttcgtt caccccagtc
     1441 acttactatt gtaagctcct ggagttcact cgcttgccgc cttcctgcaa ctcgaaatcc
     1501 atagagtaaa taaacatcac ctttcagtgc gctattgcag ccgcctgaaa gaatagtgta
     1561 tcgacctaca tcacagaccg caggaaaggg tcaatataat gattccacag atttcccagg
     1621 cacccggcgt cgttcaactg gtgcttaatt ttttgcaaga gctggagcaa caaggtttta
     1681 ccggcgatac ggcgacaagt tatgccgatc gtctgacaat gtcgaccgac aacagtattt
     1741 accaacttct ccccgatgcg gtggtatttc cgcgttcaac cgcagatgtg gcgctgatcg
     1801 cccgtcttgc cgcgcaggaa cgctattcat cgctgatctt taccccccgc ggcggcggca
     1861 ccggcactaa cggtcaggcg ctcaaccagg ggattattgt tgatatgtcc cgccatatga
     1921 accgcatcat cgaaattaac cctgaagagg gctgggtgcg cgttgaggcc ggggtgataa
     1981 aagatcaact caatcagtac ctgaaaccgt tcggctactt ttttgcacca gaactttcga
     2041 ccagcaaccg ggcaacgctc ggcgggatga tcaataccga tgcatccggt cagggatcgc
     2101 tggtctatgg caaaacgtca gatcacgtac ttggcgtacg cgcggtgttg ttggggggcg
     2161 atattctcga tacgcaacct ttacccgtcg aactggcgga aacgctgggt aaaaccaata
     2221 ccacaatcgg gcgaatttat aacacggttt atcaacgttg ccgtctgcaa cgccagttaa
     2281 tcatcgacaa cttccccaaa cttaaccgct ttcttaccgg ttacgatctg cgtcatgtct
     2341 ttaacgatga gatgaccgag ttcgacctga cgcgcattct gacgggttca gaagggacgc
     2401 tggcctttat taccgaagcg cggctggata ttacgccctt gcctaaagtg cgccgtctgg
     2461 tgaacgtcaa atatgactct tttgactccg cgctgcgtaa cgcgccgttt atggttgagg
     2521 cgcgggcgct ttcggtagag acggtggact caaaagtgct gaatctggcg cgggaagata
     2581 ttgtctggca ttccgtcagc gagttgatta ccgatgtgcc tgacaaagag atgctcgggc
     2641 tgaacattgt ggaatttgct ggtgatgatg aggcgctgat tgacgagcga gttaatgcac
     2701 tctgtgtgcg gcttgatgag ctgattgcca gccaacaggc aggtgtgatt ggctggcagg
     2761 tgtgccgcga gctggcgggg gttgaacgta tctatgcaat gcgcaaaaaa gccgttggtc
     2821 tgcttggcaa tgccaaaggt gccgctaagc caattccgtt tgctgaggat acctgcgtac
     2881 cgccggaaca tctggccgat tatattgctg aatttcgcgc gctgctcgac agccacggct
     2941 taagctacgg tatgttcggt cacgtcgacg caggtgtttt gcacgtccgt ccggcactgg
     3001 atatgtgcga tccgcaacaa gagattttga tgaagcaaat ctctgatgac gtggtggcgc
     3061 tgactgcgaa atacggtggt ttgttgtggg gcgagcacgg caaaggtttt cgcgctgaat
     3121 acagcccggc gtttttcggt gaggaacttt ttgcagaact gcgcaaagtg aaagcggcat
     3181 ttgacccgca taaccgactc aacccaggga agatttgccc gccagaaggt ctcgatgcgc
     3241 cgatgatgaa agtggacgcg gtgaagcgcg gtacattcga tcggcagatc cccattgcgg
     3301 tacgccagca gtggcgcggt gcgatggagt gtaacggcaa cggtttatgc ttcaactttg
     3361 atgcccgtag tccgatgtgt ccgtcgatga agatcaccca gaaccggatt cattcaccga
     3421 aagggcgcgc aacgctggtg cgtgaatggc tgcgtttgtt ggcggatcgc ggcgttgatc
     3481 cactcaaact ggaacaagaa ctgcctgaat ccggcgtcag tttacggacg ttaattgccc
     3541 gcacgcgcaa tagctggcat gcgaataaag gcgaatatga cttctcacac gaagtcaaag
     3601 aggcgatgtc gggctgtctg gcctgtaaag cgtgttcgac ccagtgcccc atcaaaattg
     3661 atgtgccgga gtttcgctct cgtttcctgc agctctatca cacccgttat ttacgcccgc
     3721 tgcgcgacca cctcgtcgct acggtcgaga gctacgcacc gctgatggca cgcgcgccga
     3781 aaacctttaa cttcttcatt aaccagccgc tggtgcgcaa actctcggaa aaacatatcg
     3841 gcatggttga tttgccactg ctgtcggtcc cctcgctaca acaacaaatg gtggggcatc
     3901 gctcggcaaa catgacgctg gaacagcttg aagctctcaa tgcagagcag aaagcgcgca
     3961 cggtgttggt ggtgcaggac ccctttacca gctattacga tgcgcaagtg gtggcggatt
     4021 ttgtccgtct ggttgaaaaa ttaggtttcc agcctgtgtt actgccattt tcgccaaatg
     4081 gcaaagccca gcatatcaaa ggctttctta atcgttttgc gaagacggcg aaaaagacgg
     4141 cggatttcct caaccgtatg gctaagcttg ggatgccaat ggtgggcgtc gatccggcgc
     4201 tggtactttg ttatcgcgat gaatataaac tggcgctggg cgaggaacgt ggcgagttta
     4261 acgtcttact tgcgaatgaa tggctggcaa gcgcacttga gtcacatcca gtggctacag
     4321 tcagcggtga atcatggtat ttctttggtc actgtaccga ggttaccgct ttgccgggcg
     4381 cgccagcaca atgggccgcg atatttgccc gttttggcgc gaaactggaa aatgtcagcg
     4441 tgggttgctg cggcatggca gggacttacg gacatgaagc gaaaaaccat gaaaattcgc
     4501 tcgggatcta tgagttatcc tggcatcagg ctatgcagcg actgccgcgt aaccgctgtc
     4561 tggcgaccgg atattcctgc cgtagccagg taaaacgggt tgaaggcacg ggggtacgcc
     4621 atcctgtgca ggctttactg gagattatta aatgatatgg aaacggaaaa tcaccctgga
     4681 agcactgaat gctatgggtg aaggaaacat ggtggggttc ctggatattc gctttgaaca
     4741 tattggtgat gacacccttg aagcgacaat gccagtagac tcgcggacaa agcagccttt
     4801 cgggttgctg catggaggag catccgtggt actggccgaa agtatcggtt ccgttgccgg
     4861 ttatttatgt accgaaggtg agcaaaaagt ggttggtctg gaaatcaatg ctaatcacgt
     4921 ccgctcggca cgagaagggc gggtgcgcgg catatgtaaa ccgttgcatc tcggttcgcg
     4981 tcaccaggtc tggcagattg aaatcttcga tgagaaaggg cgtttgtgct gttcgtcacg
     5041 attgacgacc gccattttgt gacgttgtca ccagaaaagt gtgacgcata ttgcttttgg
     5101 tcattatctg cacagtaaac ggtggtatac tgtgcacgtc ttgtctaaaa gcgaggatgc
     5161 tatgtctact caacttgatc ccacccagct ggccattgaa tttttacgcc gtgaccagag
     5221 caatctttca cctgcccaat atctcaaacg tctgaaacaa ctagaacttg aatttgctga
     5281 cctgcttact ctctcttcta ctgaattaaa agaagaaatc tactttgcct ggcgtctggg
     5341 cgtgcattga tcctgactta atgccgcaag ttcgccaaat cgacctggat taacctccgt
     5401 ccaggttcga tgaccttctt aatactgctt atctaccgcc tgatattgtt caaaaataat
     5461 actaatcgca gtttgtgtta aaacggcggg ttagctttat gagattattc ttatcgcccc
     5521 ttcaagagct aagccactga gagtgccgga gataagcgcc ggatggggta gaaaccctta
     5581 agcctgtgtc gcacagactt aagggttttc ttatttctga acgctgcact tttttaccta
     5641 tctcttaaat agtcattttc aatacaactt tttctttttc atcacagcct taatgctcct
     5701 tagataataa ccattatcta acaatgagat acctaattct tagcgtgcct gttaacccac
     5761 aaatcagggt ctatgcttat taaaagcagg caaaaagcat gttttacggt aaagcccctg
     5821 cgtttgctgg gttgaactga taatcattat cactagcatg ctgttatacg ctgaaagcga
     5881 tgaagtgagg taaatcgatg gacatgcatt caggaacctt taacccacaa gatttcgcct
     5941 ggcaaggctt aacgctgaca cccgcagcgg cgatacacat ccgtgagctg gtggcaaagc
     6001 agccgggtat ggtcggcgtg cgcttaggcg tgaagcaaac gggctgcgcg ggctttggct
     6061 atgtgctcga cagtgttagc gagccggaca aagacgatct gctgtttgaa cacgacggcg
     6121 cgaagctgtt tgtcccgctg caagcgatgc cgtttattga tggcacggaa gtcgatttcg
     6181 ttcgtgaagg acttaatcag atattcaaat ttcacaaccc taaagcccag aatgaatgtg
     6241 gctgtggcga aagctttggg gtataggcgg tactatgtct cgtaatactg aagcaactga
     6301 cgatgtcaaa acctggaccg gcggcccgct gaattataaa gaaggattct tcacccagtt
     6361 agccaccgat gagctggcaa aggggataaa cgaagaggtg gtgcgcgcaa tttcggcgaa
     6421 gcgtaatgag ccggagtgga tgctggagtt tcgtctaaac gcctatcgcg catggctgga
     6481 gatggaagaa ccgcactggt tgaaagcgca ctacgacaag ctgaattatc aggattacag
     6541 ttactactca gcaccatcgt gcggtaattg tgacgacact tgcgcgtctg aacttggcgc
     6601 ggtgcagcaa actggcgcga acgccttttt aagtaaagag gtggaggcgg cgtttgagca
     6661 gttgggcgtt cccgtgcggg aaggcaaaga ggtggcggtg gatgccattt tcgactctgt
     6721 ttcggttgcc actacttatc gcgaaaaact ggcggagcag ggaattattt tctgttcctt
     6781 tggtgaggcg atccacgatc atccggaact ggtgcgtaaa tatctcggca ctgtggtgcc
     6841 gggaaatgac aacttctttg ccgcgcttaa tgcggcggta gcctctgacg gtacgtttat
     6901 ttatgtgcct aaaggcgtgc gctgcccgat ggaactttcc acctattttc gcattaacgc
     6961 ggaaaaaacc gggcagtttg agcgcaccat tctggtggcc gacgaagaca gctacgtcag
     7021 ctacattgaa ggctgttccg ctccggtgcg tgacagctat cagttacacg cggcggtggt
     7081 ggaagtcatc atccataaaa acgccgaggt gaaatattcc acggtacaaa actggttccc
     7141 tggcgataac aacaccggcg gtattctcaa tttcgtcacc aagcgtgctt tgtgcgaagg
     7201 cgaaaacagc aaaatgtcat ggacgcaatc agaaaccggg tcagcgatta cgtggaaata
     7261 tcccagctgc attttgcgcg gcgataactc cattggtgag ttttactcag tggcgctgac
     7321 cagcggtcat cagcaagcgg ataccggcac caagatgatc cacatcggta aaaacaccaa
     7381 atcgaccatt atctcgaaag ggatctctgc cggacatagt cagaacagtt atcgcggctt
     7441 agtgaaaatc atgccgacgg caaccaatgc gcgcaatttc actcagtgcg actcaatgct
     7501 gattggcgct aattgtgggg cgcatacctt cccgtatgtc gaatgtcgca ataacagcgc
     7561 acaactggag cacgaggcaa cgacatcacg tattggtgaa gatcaactgt tttactgcct
     7621 gcaacgcggg attagcgaag aagacgccat ctcgatgatt gttaacggtt tctgcaaaga
     7681 cgtgttctca gaactgccgc tggaatttgc cgttgaagca caaaaactcc tcgccatcag
     7741 tcttgaacac agcgtcggat aaggaataaa catgttaagt attaaagatt tacacgtcag
     7801 cgtggaagat aaagctatcc tgcgtggatt aagcctcgac gttcgtcccg gcgaagttca
     7861 cgccattatg gggccaaacg gttcgggcaa aagtacctta tcggcaacgc ttgccgggcg
     7921 agaagattat gaagtgacgg gcggcacggt tgagttcaaa ggcaaagatt tgcttgcgct
     7981 gtcgccggaa gatcgtgcag gcgaaggcat ctttatggcc ttccagtatc cggtggagat
     8041 tcccggcgtc agtaaccagt ttttcctgca aacggcactt aatgcggtgc gcagctatcg
     8101 cggccaggaa acgctcgacc gctttgattt tcaggatttg atggaagaga aaatcgctct
     8161 cctgaagatg ccggaagatt tattaacccg ttcggtaaac gttggttttt ccggcggcga
     8221 gaaaaagcgc aacgatattt tgcaaatggc ggtgctggaa ccggagttat gcattcttga
     8281 tgaatcggac tccgggctgg atattgacgc attaaaagtg gttgccgatg gcgtgaactc
     8341 gctgcgtgac ggcaagcgct cattcatcat tgttacgcac taccaacgca ttctcgacta
     8401 catcaagcct gattacgtcc atgtgctata tcagggacga attgtgaaat ccggcgattt
     8461 cacgttggtc aaacaactgg aggagcaggg ttatggctgg cttaccgaac agcagtaatg
     8521 cgctgcaaca gtggcatcac ttgtttgaag ctgaagggac aaaacgctca ccgcaagcac
     8581 agcagcattt acaacaattg ctgcgtaccg gactgccgac acgtaaacat gaaaactgga
     8641 aatatacgcc gctggaaggg ctgaccaata gtcagtttgt cagcattgcg ggagatatat
     8701 ccccacagca gcgtgatgcc ttagcgttaa cgttagacgc cgtgcggctg gtgtttgtcg
     8761 atggacgtta cgtgcccgcg ctgagcgatg caattgaagg cagcggatat gaagtgagca
     8821 ttaacgacga ccgtcagggg ttacccgacg ctattcaggc ggaagtgttt ctgcatttga
     8881 cggaaagcct ggcacaaagc gtgacgcata tcgccgtgaa gcgcggtcaa cggccggcaa
     8941 agccattgct gttaatgcat atcacccagg gcgtggcagg tgaagaggtg aacactgccc
     9001 attaccggca tcatctggat ctggcggaag gtgccgaagc aacggtgatc gaacattttg
     9061 tcagccttaa tgatgctcgt cattttaccg gggcacggtt cactatcaat gtcgcagcga
     9121 acgcccactt gcagcatatc aagctggcgt ttgaaaaccc gctcagtcac cactttgctc
     9181 ataacgattt gttgctggct gaggatgcca ccgcatttag ccatagtttc ctgctgggtg
     9241 gcgcagtgtt acgacacaac accagtacgc aactcaatgg cgaaaacagc acgctgcgga
     9301 tcaatagcct ggcgatgccg gtgaaaaacg aggtgtgtga tacccgtacc tggctggaac
     9361 acaataaagg tttttgtaac agccgacagt tgcacaaaac tatcgtcagc gacaaaggcc
     9421 gcgcggtatt taacggtttg atcaacgtcg cgcagcacgc catcaaaacg gatggtcaga
     9481 tgaccaacaa taatctgctg atgggcaaaa tggcggaagt ggatacaaaa ccgcagctgg
     9541 aaatctatgc agatgatgtg aaatgcagcc acggcgcgac ggtggggcgt attgatgatg
     9601 aacagatgtt ctatctgcgc tcgcgcggga tcaatcagca ggatgcccag cagatgatca
     9661 tttacgcctt tgctgccgaa ctgacggaag cactgcgtga tgaggggctt aaacagcagg
     9721 tgctggcccg aatcggtcaa cggctgccag gaggtgcaag atgacttttt ccgtcgacaa
     9781 agtgcgggcc gactttccgg tgctttctcg tgaggtgaac ggtttgccgc tggcttatct
     9841 cgatagcgcc gccagtgcgc agaaaccaag ccaggtgatt gacgccgagg ctgagtttta
     9901 tcgtcacggc tacgcggcgg tgcatcgcgg tattcatacc ttaagcgccc aggcgaccga
     9961 gaaaatggag aacgtgcgca agcgggcatc gctgtttatt aatgcccgtt cggcggaaga
    10021 gctggtgttc gtccgcggca cgacggaagg gatcaatctg gtcgccaata gttggggcaa
    10081 cagcaacgtg cgggcgggcg ataacatcat catcagtcag atggagcacc acgctaacat
    10141 tgttccctgg cagatgcttt gcgcacgcgt tggcgcagag ctgcgtgtga tccctctcaa
    10201 ccccgacggt acgctgcaac tggagacgct gcctacgctg tttgatgaga aaactcgcct
    10261 gctggcaatt actcatgtct ccaacgtgct gggaacagaa aatccgctgg cggaaataat
    10321 cacgcttgcg caccagcatg gcgcaaaagt gctggtggat ggcgctcagg cggtgatgca
    10381 tcatccggtg gatgttcagg cgctggattg cgacttttac gtgttctccg ggcataaact
    10441 gtatggcccc accggaattg gcattcttta tgtgaaagaa gccttgttgc aggagatgcc
    10501 gccgtgggaa gggggcggtt ctatgatcgc caccgtcagc ctgagtgaag gcactacctg
    10561 gaccaaagca ccatggcggt ttgaagccgg tacacccaat accgggggca ttattggtct
    10621 tggcgcggcg ctggagtatg tttcggcgct ggggcttaat aacatagccg agtatgaaca
    10681 gaatctgatg cattatgcgc tatcacagct ggaatctgta ccggatctca ctctctatgg
    10741 cccacaaaac aggcttggcg ttattgcttt taatctcggt aaacaccacg cctatgatgt
    10801 tggcagtttt ctcgataatt acggcattgc tgtgcgtacc ggacatcact gcgctatgcc
    10861 attaatggcc tattacaacg tccctgcgat gtgtcgggcg tcgctggcca tgtataacac
    10921 ccatgaagaa gtggatcgtc tggtgaccgg cctgcaacgt attcaccgtt tgctgggata
    10981 acagggaggc actatggctt tattgccgga taaagaaaaa ttgctgcgta attttttacg
    11041 ctgcgccaac tgggaagaga aatatctcta cattattgag ctgggccagc gtctgactga
    11101 attacgcgac gaagaccgaa gcccacaaaa tagcattcag ggctgccaga gtcaggtgtg
    11161 gattgtcatg cgccagaatg cgcacggaat tattgaattg cagggcgaca gcgatgcggc
    11221 gattgtgaaa gggcttattg cggtagtctt tattctctac gatcagatga cgccgcagga
    11281 tattgtcagt ttcgatgtgc gtccgtggtt tgaaaaaatg gcactcaccc aacatctcac
    11341 cccatctcgt tcacaaggtc tggaagcgat gattcgcgca attcgcgcca aagccgctgc
    11401 acttagctaa actaaaggga cagctttcat ccggttggct cacattgccg gatggtcaat
    11461 gtttacactg acctgcacag gagtgttttg gtttcaggtg aacataagcg ccatccgacg
    11521 tttcagcgtg agtctccggc aaatacagga ggtttacaat gaaacgcgcg tctttgctta
    11581 cactcacgct tatcggcgct tttagcgcca tccaggctgc ctgggcggtt gattatccgc
    11641 taccaccaac cggaagccga ctggttgggc aaaatcaaac gtatacggtg caagaagggg
    11701 ataaaaacct tcaggctatt gcccgacgtt ttgatactgc ggcaatgttg atccttgaag
    11761 ccaataacac tatcgtcccg gtgccaaaac ctggtacgac gataactatt ccttcacaac
    11821 tgttattacc tgatgcaccg cgtcagggga ttatcgttaa ccttgcagag ctgcgccttt
    11881 attattatcc gccgggagaa aatattgtgc aggtttatcc aataggtatt ggattgcagg
    11941 ggctggaaac gccggtgatg gaaacgcgtg ttgggcagaa aatccctaac ccaacctgga
    12001 cgcctacggc aggcattcgt cagcgttcgc tggagcgtgg cattaaatta ccgccagtcg
    12061 ttcctgccgg accaaataac ccgctaggac gttacgcact gcgcctcgcg catggtaatg
    12121 gcgaatacct cattcatggc accagtgcgc cggacagcgt tggtttgcgc gtcagttcag
    12181 ggtgtattcg catgaatgca ccggatatta aagccttgtt ctccagcgtg cggacgggaa
    12241 cgccggtgaa agtgatcaac gaaccggtga aatattccgt ggaacctaac gggatgcgtt
    12301 atgttgaagt acatcgacca ctatcagcag aagaacagca aaacgttcag acaatgccat
    12361 acacactgcc agcaggcttt acgcaattta aagacaataa ggctgtagat cagaagttag
    12421 tcgataaagc gttgtatcgt cgggcagggt atccggtttc ggtgagcagt ggagcaactc
    12481 ccgcagccag caatgcgcct tcagtagagt cagcgcagaa tggtgaacca gagcaaggga
    12541 atatgttacg cgcgacgcag tagtggtaaa tagcagacaa aaaaatggcg cacaatgtgc
    12601 gccatttttc acttcacagg tactattact tgcggtattt agtagccatg ttgtccagac
    12661 gctggttagc acgagctgcg tcatctttag cagcctgaac gtcggaacgc attgcgttca
    12721 cgtcgttgct cagctggtca actttagcgt tcagagtctg aacgtcagaa gacagctgat
    12781 cgattttagc gttgctggag caacctgcca gcagagtaga acccaggatt accgcgccca
    12841 gtaccagttt agtagctttc attattaata ccctctagat tgagttaatc tccatgtagc
    12901 gttacaagta ttacacaaag ttttttatgt tgagaatatt tttttgatgg gaaggcactt
    12961 atttttgatc gttcgctcaa agaagcatcg aacgctgggt tttagataaa aaaactgaga
    13021 gaaaacagga tgcttccatc ggattcatct tagataaagt gagattatat agtgaaatcc
    13081 gatttgattt tttattgctt ctggttatcg attaaataaa aaagcgccca tcagggcgct
    13141 tcgatataca aattaattca caaaagcaat attacaggac gtgaacagat gcggtgttag
    13201 tagtgccgct cggtaccagt gcaccagaaa ccataactac gacgtcacct ttgtgtgcca
    13261 gaccgctctg cagagccagt tctttaccca gacggtagaa atcatcagta gaagtgatct
    13321 ctttaaccag ctgcggcaca acgcctttgc tcagtaccaa ctgatgagcc gttttttcgt
    13381 tggtggtcag tgccagtatg gtggcatccg ggaagtattt acgtactgcg cgagcagatt
    13441 taccgccctg ggtagcaacc acgatcagcg gagcatccag tttttcagca gtttcaacgg
    13501 caccacggca taccgcttcg gtaatgcgca gtttacggtt gtcattgttg aactcgagac
    13561 ggctgttcat cacgcggtcg gtacgttcgc agatggtcgc catgatagaa accgcttcca
    13621 gcgggtattt accttttgcg gattcaccag acagcatcac tgcgtcagta ccgtcgagga
    13681 tggcgtttgc aacgtcaccg gcttctgcgc gagtcgggcg tgggtttttg atcatggaat
    13741 ccagcatctg ggtcgcagtg ataacgactt tacgtgcacg gatacatttt tcgatcatca
    13801 tcttctgggc gaagataact tcttctaccg gaatttctac acccaggtcg ccacgcgcaa
    13861 ccatgatgcc gtcagaggct tcgaggattt cgtcgaagtt gttgaggcct tcctggtttt
    13921 cgattttgga gatgatgtgg atgttttcgc cgccgtgcgc tttcaggtgc tcacggattt
    13981 cgataacgtc agaacgctta cgaataaagg aagcagcaac aaagtctacg ccttgttcgc
    14041 aaccaaagat caggtcctgt ttgtcttttt cagccagtgc tggcagggca atggaaacgc
    14101 caggcaggtt cacacctttg ttttcgccca ggtcgccgtt gttcagcact ttacagataa
    14161 ctttgttacc ttcaatggca gtaacttcca taccgatcag accatcgtca accagtacgg
    14221 tgttgccaac agacaggtca gtagtgaaac cttcatacgt taccgcaacc atttcgctgt
    14281 tgccgataac agatttatca gtggtgaaag taaaggtctg acccgctttc agagaaacgt
    14341 cgttaccgcc ttccagtttc atggtgcgga tttccggacc tttggtatca agcaggatag
    14401 cggcggtttt accagttttg ctcatcacgt tgcgcagatt ctgaatgcgc tgaccgtgtt
    14461 ctgcatagtc accatgagag aagttcagac gcataacgtt catgccagcg tccagcattt
    14521 tagctaacat ctcttcagat tcggttttcg gtccgatggt gcaaacaatt ttggtctttt
    14581 tcatgacagt cttagtcttt aagttgagaa ggatgggaga aacttgcttt ctgggcgcac
    14641 tgccggaaag cgaatccggt attacgaaag tggtggtgcc ttgctctaag gataggtgac
    14701 atcgaaaaag cgtgcagagg aatgtgtgct tgtgtctcag cccaacgtga gtgcctgttt
    14761 ttgagtcgtt gagttctaca gtccaaagtg ctgaaaccat tcaagagtca attggcgcgc
    14821 attatacgct gaaattaatc caaaaggaaa acaaaaacag cgtttcaaaa aatatacaac
    14881 ttgttagaca ataaattgtt atcaaagatt taacgttcca gggaaaaggt tacgcaaaaa
    14941 cttcgctgtt tgcgcaacgt ctggtgccga agttgtcagt gtgaggataa gtgaaagttc
    15001 atccggtgta taaatatatc atcgctcaat tgctacagtt aaaataattt atcgcgttat
    15061 ttgtctgatt ttgtttgatt tttgtttaat tggttagatt gatatatgct gatagttggc
    15121 atgataaata ggtataaaga atgggaaatc ggacaaaaga ggatgaactg taccgagaaa
    15181 tgtgcagagt tgtcggtaaa gttgtgctgg aaatgcgcga tctggggcag gaaccaaagc
    15241 atattgttat cgcaggcgta ttacgtactg cattagccaa taaacgcatt cagcgcagtg
    15301 agctggaaaa gcaggcgatg gaaaccgtca ttaacgcact ggtaaaatga ccattcagga
    15361 tgaggcggta agcttcatcc tgatattctt gcttcagttg cggtccagtt aatcattcta
    15421 aataacaact gctgaaatta tacga
//
LOCUS       DAZPJR010000093        15407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPJR010000093 DAZPJR010000000
VERSION     DAZPJR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.094"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..454
                     /locus_tag="ACHVB7_002699"
     CDS             <1..454
                     /locus_tag="ACHVB7_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1914643.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            670..759
                     /locus_tag="ACHVB7_002700"
                     /pseudo
     CDS             670..759
                     /locus_tag="ACHVB7_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHVB7_002701"
     CDS             complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHVB7_002701"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR1914644.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHVB7_002702"
     CDS             complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHVB7_002702"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR1914645.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHVB7_002703"
     CDS             complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHVB7_002703"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1914646.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHVB7_002704"
     CDS             complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHVB7_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR1914647.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3727..4515
                     /gene="modE"
                     /locus_tag="ACHVB7_002705"
     CDS             3727..4515
                     /gene="modE"
                     /locus_tag="ACHVB7_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR1914648.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4583..6055
                     /gene="modF"
                     /locus_tag="ACHVB7_002706"
     CDS             4583..6055
                     /gene="modF"
                     /locus_tag="ACHVB7_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR1914649.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6316..7332
                     /gene="galE"
                     /locus_tag="ACHVB7_002707"
     CDS             6316..7332
                     /gene="galE"
                     /locus_tag="ACHVB7_002707"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR1914650.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7342..8388
                     /gene="galT"
                     /locus_tag="ACHVB7_002708"
     CDS             7342..8388
                     /gene="galT"
                     /locus_tag="ACHVB7_002708"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR1914651.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8392..9540
                     /gene="galK"
                     /locus_tag="ACHVB7_002709"
     CDS             8392..9540
                     /gene="galK"
                     /locus_tag="ACHVB7_002709"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR1914652.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9534..10574
                     /gene="galM"
                     /locus_tag="ACHVB7_002710"
     CDS             9534..10574
                     /gene="galM"
                     /locus_tag="ACHVB7_002710"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR1914653.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHVB7_002711"
     CDS             10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHVB7_002711"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR1914654.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHVB7_002712"
     CDS             complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHVB7_002712"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR1914655.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13056..13436
                     /locus_tag="ACHVB7_002713"
     CDS             13056..13436
                     /locus_tag="ACHVB7_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR1914656.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13550..14491
                     /gene="zitB"
                     /locus_tag="ACHVB7_002714"
     CDS             13550..14491
                     /gene="zitB"
                     /locus_tag="ACHVB7_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR1914657.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHVB7_002715"
     CDS             complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHVB7_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR1914658.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15245..>15407)
                     /locus_tag="ACHVB7_002716"
     CDS             complement(15245..>15407)
                     /locus_tag="ACHVB7_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1914659.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4044 c         3851 g         3596 t
ORIGIN      
        1 cggcctttgc atggatcttg cactggtaac aggacagcgc gaaggagatc tggcggctat
       61 gagatgggag gatataagag atggcaggct atacgtggaa caacaaaaaa caggggcaaa
      121 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      181 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      241 gacaatcgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      301 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      361 cggtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      421 tgacggacga gggagggaat ggaaagacat ttaaaattaa tttcgaatga tttcgaaaca
      481 ccacttgtaa ttaattgatt ataaatgatt aattcctaat gggcatttgc ttcatgaatg
      541 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      601 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      661 aaattcttaa atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
      721 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
      781 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
      841 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
      901 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
      961 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1021 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1081 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
     1141 cagccacgga ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac
     1201 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     1261 gctgacatac aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag
     1321 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     1381 tgctgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     1441 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     1501 gaccatactt ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacag
     1561 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc agataccttt
     1621 ttcggcatca tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag
     1681 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     1741 gatgccattg gcgggcagtg tttcattaat ggtcaggcaa tggttgccca acgtctggga
     1801 aaaattcagc tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     1861 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     1921 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     1981 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2041 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     2101 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     2161 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     2221 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     2281 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     2341 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     2401 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     2461 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     2521 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     2581 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     2641 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     2701 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     2761 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     2821 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     2881 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     2941 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3001 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3061 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     3121 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     3181 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     3241 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     3301 aacgttatat aggtgacaat gtaacggcta gctcagaatt ttcccagcag ttattgctaa
     3361 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     3421 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     3481 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     3541 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     3601 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     3661 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     3721 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     3781 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     3841 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     3901 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     3961 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4021 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4081 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     4141 gatcatgatg acattcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     4201 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     4261 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     4321 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     4381 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     4441 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     4501 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     4561 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     4621 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     4681 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     4741 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     4801 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     4861 tggcgaagat gacaccggac gcactacggc tgaaatcatt caggatgaag taaaggatgc
     4921 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     4981 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
     5041 gcctgacttg ttgattcttg atgagtcgtt cgatggcctg gatgttgcct cacgtcagca
     5101 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     5161 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     5221 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
     5281 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     5341 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     5401 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     5461 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     5521 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     5581 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     5641 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     5701 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     5761 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
     5821 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     5881 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     5941 gcaattgtta tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6001 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6061 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     6121 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     6181 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     6241 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     6301 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
     6361 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     6421 agtaagcgca gcgtactgcc tgttatcgag cgcttaggcg gcaaacatcc aacgtttgtt
     6481 gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac
     6541 accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa
     6601 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
     6661 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     6721 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
     6781 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
     6841 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     6901 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     6961 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7021 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7081 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
     7141 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
     7201 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     7261 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     7321 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     7381 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     7441 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     7501 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac
     7561 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     7621 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     7681 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     7741 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     7801 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     7861 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     7921 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     7981 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8041 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     8101 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     8161 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     8221 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     8281 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     8341 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     8401 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     8461 caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
     8521 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     8581 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     8641 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     8701 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     8761 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     8821 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     8881 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     8941 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
     9001 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9061 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
     9121 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
     9181 cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
     9241 caaggcgatc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
     9301 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agcagtgatt
     9361 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
     9421 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
     9481 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
     9541 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
     9601 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
     9661 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
     9721 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
     9781 gccgttatac ctttgacggt gaaacggtga cgctttcgcc aagtcagggc gttaaccagc
     9841 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
     9901 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
     9961 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10021 cagttgataa atcttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10081 agcagtctga cgtgcgcaat cacaagttgc agattctggc gggcgaatat ctgccggttg
    10141 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacctct tttgatttcc
    10201 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    10261 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    10321 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccc gctcttcaat
    10381 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact
    10441 ggcaaggact ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    10501 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    10561 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc
    10621 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    10681 attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa
    10741 ttattatcat aaaaagataa tttgaggagt aagtatatgg ctgtaactaa gctggttctg
    10801 gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg
    10861 gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa
    10921 ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg
    10981 aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac
    11041 gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaaccgccga aaagtatggc
    11101 gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga ctccgccgga actgactaaa
    11161 gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagagctg
    11221 ccgctgactg aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact
    11281 attctaccgc gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg taactcttta
    11341 cgtgcgctgg tgaaatatct tgataacatg agtgaagaag agattcttga gctgaatatc
    11401 ccgaccggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat
    11461 tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgctaa ccagggtaaa
    11521 gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag tcggcttttt
    11581 tgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg
    11641 tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc
    11701 gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca gccaatgcag
    11761 gcatcggtga tgctcttacc gtaggccagc ggctccccgc tatcgaggct ctgattgcct
    11821 tccaccagat ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc
    11881 tggcaaacgt cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg
    11941 ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca
    12001 gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatca
    12061 ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tagttacgga caggaagcag
    12121 tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc gtctgtgcca
    12181 tttttaaaac ctaccggaca agaaagacca gacgccagtt cgcggtgcac ctgcgattcc
    12241 gtggtacgtg caccaattgc gccccagctc atcaggtcag cgagatactg aggagtgatc
    12301 atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta
    12361 cgggctatac gcagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc
    12421 aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc
    12481 agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc tttagccgcg
    12541 acaggatcat gaattgagca cgggccaatc accaccaaca ggcgatcatc attacctttc
    12601 aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagttacg
    12661 gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa
    12721 tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa
    12781 tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat
    12841 taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca
    12901 atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata
    12961 aactggctga aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag
    13021 gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat
    13081 tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag
    13141 cgcaaactaa taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg
    13201 cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggcg
    13261 gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaag
    13321 agcacaagaa caccatgtgc aaagatggtc gatgtccgga tatcaataaa aaggtacaaa
    13381 tcggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct
    13441 gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag
    13501 cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc
    13561 acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc
    13621 tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc
    13681 tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc
    13741 cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct
    13801 aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt
    13861 gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat
    13921 gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca
    13981 cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ttgcatgtgc tcggggacct
    14041 gttgggttcg gttggggcta ttattgccgc gttgattatt atctggaccg gctggacacc
    14101 tgctgacccc attctctcga tagtggtgtc gcttctggtt ctgcgtagcg cgtggcgact
    14161 gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga
    14221 actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt
    14281 atggatggta ggcgagaagc cggtgataac gctgcatgtg caggtgatcc cgccacacga
    14341 tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca
    14401 cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg
    14461 ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg
    14521 tgcgctgttg atccacatcc gactgccgtt gagcgcaata aaggtcagga tgatgtactc
    14581 cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat
    14641 cacccacaac agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga
    14701 taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac
    14761 aacctgtaat cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac
    14821 cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag
    14881 cgccttcggc aatggtaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg
    14941 cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct
    15001 ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa
    15061 gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt
    15121 gcctaccgct tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag
    15181 gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta
    15241 ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc
    15301 tctctcgcag cctttcatca agaacctcgt gattgcttcc ttcctgttct aatgcctctg
    15361 cgatggactg aaggccattc atggccatcc acggacaatg cgcgcag
//
LOCUS       DAZPJR010000094        15380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPJR010000094 DAZPJR010000000
VERSION     DAZPJR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.095"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHVB7_002717"
     CDS             <1..1015
                     /locus_tag="ACHVB7_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1914660.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHVB7_002718"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHVB7_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR1914661.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHVB7_002719"
     CDS             1797..1904
                     /locus_tag="ACHVB7_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR1914662.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHVB7_002720"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHVB7_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR1914663.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHVB7_002721"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHVB7_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR1914664.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="ACHVB7_002722"
                     /pseudo
     CDS             complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="ACHVB7_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="ACHVB7_002723"
     CDS             complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="ACHVB7_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR1914665.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="ACHVB7_002724"
     CDS             complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="ACHVB7_002724"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR1914666.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8365..10080
                     /gene="dld"
                     /locus_tag="ACHVB7_002725"
     CDS             8365..10080
                     /gene="dld"
                     /locus_tag="ACHVB7_002725"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1914667.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="ACHVB7_002726"
     CDS             complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="ACHVB7_002726"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR1914668.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMLVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11225..11812)
                     /locus_tag="ACHVB7_002727"
     CDS             complement(11225..11812)
                     /locus_tag="ACHVB7_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR1914669.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11982..12560
                     /locus_tag="ACHVB7_002728"
     CDS             11982..12560
                     /locus_tag="ACHVB7_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1914670.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12690..13451)
                     /locus_tag="ACHVB7_002729"
     CDS             complement(12690..13451)
                     /locus_tag="ACHVB7_002729"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1914671.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="ACHVB7_002730"
     CDS             complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="ACHVB7_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR1914672.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15164..15247
                     /gene="yohP"
                     /locus_tag="ACHVB7_002731"
     CDS             15164..15247
                     /gene="yohP"
                     /locus_tag="ACHVB7_002731"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR1914673.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3753 a         4188 c         3853 g         3586 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgcagtg
     3781 gtaactgaac atgcaggaat cgctgcgccc cgctcatgcc catcgctctg gcgctctcca
     3841 gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg
     3901 catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg ccgagcgtcc
     3961 ccagccacgg aaaggccgta accagcgcgg caagcggcgc aatcaacagg ccaaagagcg
     4021 ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc tgccgagcag
     4081 tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac
     4141 caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt
     4201 attctttcat tagtgagaga tcgttaagcg tgccggagta cagcaaccac agcggaataa
     4261 tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt cggcggatgg
     4321 catcactaca ggccagcaat gccagcgccg cggccagcca gaaaccactg ccgaggctgg
     4381 tacgcgccag cgcactgcca ttttgcgcca gttgggtcgc cgcctttcct gctccccaca
     4441 ccagcaatac gaagacgaat tgcgccagaa tgagtgcaaa aatgctgcct tttttagcgg
     4501 gaataaaaca ggctgtcagc caggcgcaac caacgcccac cagcatccag atcgtttgcg
     4561 gccacagttg ccagagatga cgcccttcac ccgagactaa acgattaggc gcgtaactga
     4621 taaacggcag cgccgctgcg attgccgtca gcaacagcag cagcgccaga acaggattaa
     4681 tacgtagata agtcacggga aattacttcg tccacccttt ttgtttcagg tagtcggcag
     4741 ccactttttt ggcatccagt ccttccactg caatgctggc attcagttgc tgcaatgttt
     4801 ttgcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga tactctttca
     4861 gcaccgactc acgcaccact ggtgcaggcg cgtagatagg ttgcacgcct ttcggatcgc
     4921 ttaaggtttg cagccccagc gccgcgaccg gaccgtcagt gccgtaagcc attgcggcat
     4981 taacgccaga agtttgctgg gcagcggctt taatcgttac cgccgtgtcg ccgccagcca
     5041 gtgacagtaa ctgatcctga tcgagtttaa agtcgtaggc tttttcaaac gcgggtaacg
     5101 catcggcgcg ttcgataaac tctgccgagg ctgccagttt gaaggtgccg ccctctttca
     5161 gataacgact caggtcggca agcgaagtga gtttgttttt ctctgccaca tcctgacgca
     5221 cggcgatggt ccaggtgtta ttcgctggtg cgggcgtcag ccagattaac ttgttttgct
     5281 ctgcatcgag ttttttgact ttctcgtaac cttgctgcgc gtttttccat gccgcatcgt
     5341 tttcatcttt aaagaagaaa gcgccattac cggtatattc cggatagata tccagttcac
     5401 cggaagtaat cgccccgcgc accacaggag tcgttccgag ttgcacttta ttcaccgtcg
     5461 gtacgccgtg gctttccagc acctgcaaga tgatgttgcc gagtagcgcg ccttcggtat
     5521 cgatttttga accgacttta acgggggaag ccgcctgtag cggcaggctc acggctgcca
     5581 acataaccag tgaacctgcc cagacctttg agagtggcat gatgctttcc tcattctttt
     5641 actgttgttt tcagcgaatt aagagaaaag catagttgat aatgacagag ttagcctgag
     5701 cggaaggatt cagttgcaga atcagataaa tacttaaagg gcatattttg cccggcgttt
     5761 cttgccggat gcggcgtaaa cgccttatcc ggcctacaaa ggtcgaaaat tcagcaagtt
     5821 aagatatgaa catcaggcaa agggcgcaaa cgtcgcgccc ttactaaagc attacagcaa
     5881 ctcaaactcg cctttcttaa cgcgtgcgga atcagtgccg ataaagacat tgaacttgcc
     5941 aggttcggcg tcatatttca tctgttgatt ccagaacttc agcgcctcga tatcaatcgg
     6001 gaagctgaca gtctgagttt cgcccggttt cagggtgatt ttctcaaagc ctttcagctg
     6061 cttcactgga cggctcatgg aagccgtcac atcctgcaag tacatctgta ctaccgtggc
     6121 cccttcgcgt ttaccggtgt tcgtcacctg cacgctggcg gtcactttgc cgtcacgctt
     6181 catggtcggc gcagaaagtt tcacatcaga gacggtgaaa gtggtatagc tcagaccata
     6241 gccgaacgga taaagcgccc cgttagcttc atcaaaataa cgcgaagtgt atttgttcgg
     6301 cttgtcggca ttatacggac gaccggtatt cagatggctg tagtacaccg ggatctgccc
     6361 gacagaacgc gggaaggaca ttggcagctt gccggacggg ttgtaatcgc caaacaatac
     6421 atcggcaatt gcattaccgc cttcagtccc cgcaaaccag gtttccagaa tcgcatcagc
     6481 ctgctgatct tctttcacca gcgccagcgg acgcccgttc atcagcacca gcaccagcgg
     6541 tttaccggtg gctttcagcg ccgtaatcaa gtcacgttgg ctttgcggaa tagtgatatc
     6601 ggtacggctg gaggcctcgt gcgccatccc ctgtgcttca ccgaccacag ccaccaccac
     6661 atcagattgt ttcgccgtct gcaccgcttc atcaatcatc tcttgcggcg agcgcggatc
     6721 gactttgacc gcttcttcat actgattcag gaaatcgata atgcctttgt cactggtaac
     6781 gttcgcccct ttggcataca gcactttacc gttttcaccg acggcatttt taatcccggt
     6841 cagtacggtc acggattgat cggcaacacc ggctgcggac cagctgccca tcacgtcacg
     6901 tttactgtcc gccagtggcc caaccaccgc aatggtggcc gattttttca gcggtaacgt
     6961 ttcgagacgg tttttcagca acaccaggct ttcgcgcgcc acttcacgcg cttctttacg
     7021 gtgcaggcgg ctttcggcat tggtatccac cgggtcagac tctttcggcc ccaaatggct
     7081 gtatgggtcg ttaaacaatc ccatatcata tttaacgttc agtacatggc gggcagcatc
     7141 gtccagctct tccatcgtca ctttgccgga tttgatcaac ccaggcagat acttcgagta
     7201 atactcgtcg ctcatactca tgttgattcc ggatttcagc gccacgcgca ccgcatcttc
     7261 cgggtctgcc gccgtgccat gtttaatcag ctctttgatt gcaccgtgat cggaaacggt
     7321 gatgccttta aagccccact ggtcgcgcag aacatctttc agcagccagg aatcggaggt
     7381 ggctggcgtg ccgttcagcg agttcagcgc caccatcacc gcgccgctgc ctgcgtccag
     7441 ccccgctttg tacggcggca tataatcatt aaacaggcgc tgcggactca tatcgacggt
     7501 gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat
     7561 caccgagtag cgatctgccg ggcttttacc ctgcatcgct tccaccatgg ttttacccat
     7621 tgttgaggtg agatacgtat cttcgccaaa accttcggaa gcacgtcccc agcgcggatc
     7681 gcgcgagaca tcgaccatcg gtgcccaggt catattcagg ccatcatctg ccgcttcata
     7741 agcagagaca cgtccgaccg ttttcactgc atcgaggtta aaagacgagg ccagaccgag
     7801 gctaatcggg aacaccgtgc gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat
     7861 tttcaggcgg cttaattcca tcacctgatc ctgcatggcg cggatatcct gacgggttac
     7921 ggtgttgaaa atcgccccaa cctgaccgtc tttgatcatc tcgcggatcg cctctttcgg
     7981 gttatccgga ccgacgctga ttaagcgcag ctgaccaatt ttctcatcaa ctgtcatttt
     8041 cttaagcagt tcggtgacga acgcatcccg cgcttcgggc gttaatggat ggttgccgaa
     8101 taaatcatcc gccagtgctg gctgcagggc cagactcacc gcgattccta ctgaacatag
     8161 ccatttcata tggatttttc ttcctctgtt gcccgacgag gcagcgaaaa tggtgcaaaa
     8221 accgtagttt gccataagca tgatggagag agaaaaagaa tgctcagttt attgtctgaa
     8281 ttttcaaaat attcactcgc tgaattgtca tacaaggcgc tattctagtt tgtgatattt
     8341 tttcgccacc acaaggagtg gaaaatgtct tccatgacaa caactgataa taaagccttt
     8401 ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg
     8461 gcccgctatc gcaagggctt ccgttctggt cagggcgacg cgctggctgt cgttttccct
     8521 ggctcactac tggaattgtg gcgggtgctg aaagcctgtg tcaccgccga caaaattatt
     8581 ctgatgcagg ctgccaatac aggcctgacc gaaggatcga cgccaaacgg taacgactat
     8641 gatcgcgata tcgtgatcat cagcaccctg cgtctcgaca agctgcacgt tcttggcaag
     8701 ggcgaacagg tgctggccta tccgggcacc acgctctatt cgctggaaaa agccctcaaa
     8761 ccgctgggac gcgaaccgca ctcagtgatt ggatcatcgt gtataggcgc atcggtcatc
     8821 ggcggtattt gtaacaactc cggcggctcg ctggtgcaac gtggcccggc gtataccgaa
     8881 atgtcgttat tcgcgcgtat aaatgaagac ggcaaactga cgctggtgaa ccatctgggg
     8941 attgatctgg gcgaaacgcc ggagcagatc cttagcaagc tggatgatga tcgcatcaaa
     9001 gatgacgatg tgcgtcacga tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt
     9061 cgtgatattg aagccgacac gcccgcacgt tataacgccg atcctgatcg gttatttgaa
     9121 tcttctggtt gcgccgggaa gctggcggtc tttgctgtac gtctagatac cttcgaagcg
     9181 gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc cggaagtgct gaccgaaatc
     9241 cgccgtcata ttctggctaa cttcgaaaat ctgccggttg ccggggaata tatacaccgg
     9301 gatatctacg atattgcgga aaaatacggc aaagacacct tcctgatgat tgataagtta
     9361 ggcaccgaca agatgccgtt cttctttaat ctcaagggac gcaccgatgc gatgctggag
     9421 aaagtgaaat tcttccgtcc gcattttact gaccgtgcga tgcaaaaatt cggtcacctg
     9481 ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg ataaatacga gcatcatctg
     9541 ctgttaaaaa tggcgggcga tggcgtgggc gaagccaaat cgtggctggt ggattatttc
     9601 aaacaggccg aaggcgattt ctttgtctgt acgccggagg aaggcagcaa agcgttttta
     9661 caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc
     9721 gaagacattc tggcgttgga tatcgctctg cggcgtaacg acaccgagtg gtatgagcat
     9781 ttaccgccgg agatcgacag ccagctggtg cacaagctct attacggcca ttttatgtgc
     9841 tatgtcttcc atcaggatta catagtgaaa aaaggcgtgg atgtgcatgc gttgaaagaa
     9901 cagatgctgg aactgctaca gcagcgtggc gcgcaatacc ctgccgagca taacgtcggt
     9961 catttgtata aagcaccgga gacgttgcag aagttttatc gcgagaacga tccgaccaac
    10021 agtatgaatc cggggatcgg taaaaccagt aagcggaaaa actggcagga agtggagtaa
    10081 aaattacgga tggcatagta tcgccatccg tcattcactt aatcgttctg tgccgtctgc
    10141 cccgccgccg ccatttgggc ggctttttgt tttttatagc tcaacgctgc tgctggaaca
    10201 agcatcactt taccagtttc aatccaggta cgcaggcggc tggcatcggc aaaatgggta
    10261 tatttgccaa acgcatccat cactaccagc gctaccggtt tattattgat aaccgtacgc
    10321 atcaccagac aatggcccgc cgcattggta aaaccggttt tggttaactg aatattccag
    10381 ttatcgcgat acaccagatg gttagtattg cggaacggca gcgtatacgt cgggttagag
    10441 aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata ttgtttgctg
    10501 gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga caatccggta
    10561 ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt cattgcctta
    10621 ataaaggctt tgtaaccacc gggataatgg tgtgcaaggc ttgccgccgc gcggttttct
    10681 gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctatt cagtcgtacg
    10741 cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag tttttcatcc
    10801 agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat agacgcaatc
    10861 ggacgcacca gatccgggtg gttcgagtag atcactttgt tggtattcag atcaacaatc
    10921 atcgcgctac cggaggcaat ttcaggttgt gaagcggtgg tagcggctgc cgttttcgca
    10981 acggcctgcg gtgccaaagg cacagccagc atcagggcca ggctaaataa agaaactcga
    11041 aatttcggca tgatgagcat tcagatagtg gttcacgcgc acaggttgcg caccgccgga
    11101 gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag
    11161 gagaatcgtg aggaaatgct gcattgctga catttacgcc agcaatgcaa cgtcaaagaa
    11221 ctttctagaa caaacgataa ccgtagcccc atagtataac ggttagggcg agcagcactt
    11281 ccagtaccag cacgccaatc gccagcgtcg aactagagaa gctaaggcct tcctctttgt
    11341 tgatattcag gaagctcgga ataccaaggt aaagcaggta gccggtgtaa aacagcgcca
    11401 ccgtgccgac cagcgcacac aaccagacca gtggataaag cgccaccaga ccgcttaaaa
    11461 acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg
    11521 gataattacg cgccatccac cagatgaccc gccccattac cgcaacccca gccagcataa
    11581 cgccataaaa caggacagcc agcgccagtc cggtaaacca ggataacttc aggatagtgc
    11641 catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg
    11701 ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa
    11761 tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc ataccgcccc
    11821 cttgttgtga gttcatgaac ctgacagttc aagtataagt cagcttgtga ttattttttg
    11881 ttcgctcatg taatttgcac cgtctgatgt acgtcctttt tcagcgtgaa tgatttccag
    11941 ggtgtatgct tacaggcaac caaagggaga cagactggcc tatggatctc aatacactca
    12001 tctcacaata tggttatgcc gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga
    12061 ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccgctg gtggtacttt
    12121 ctgtggcgct tggcggcatg attggcgacc aggtgcttta tctgtgcggg cggcggtttg
    12181 gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg gcgcagaaac
    12241 ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg
    12301 tgattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga
    12361 atattctcgg cgcatttgcc tgggcgttga tttttaccac tattggttac gctggtggtc
    12421 aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg gtctggttga
    12481 ttctggtcgt ggttctggtg gtgggcgtgc gctggtggct gaaacggcgc gggaagaaaa
    12541 agccggatca tcaggcgtaa aaccattgcc ctgggtaagg cgttcgcgcc gcatccgaca
    12601 tccggtgcac catgcctgat gcgacgcttg tgcgtcttat catgcctacc aaaattttac
    12661 ccattgaaat aaccacgcac ctcactgcgc tattctgggt tgaactgcgg attcgccaac
    12721 ataaagccgc catccactat caacgactgc ccggtggtgt aatttgcgcc ctctgagcac
    12781 agccacgcta ccaggctggc aatctcatgc gttgcgccaa aacgccgcag aggaatcgaa
    12841 ggctccgcgt cgggcttcac gtcgctgtca tccatgccat tcattggcgt ggcgatcgcc
    12901 ccaggcgcga ctgcgttcac caaaatctta tgcctgacca gctccagcgc catcgttttg
    12961 gttaacccgc cgagcgcatg tttagcggct gtgtaagcgc tggcatccgg cagcggcgta
    13021 tgttcatgta ccgacgtaat gttgatgata cgaccgccct gcccttgttt caccatctgg
    13081 cgggccgcaa tttgtgagca taaaaatgca ccatcgacat caacggtaaa aatcttgcgc
    13141 cactcatcga aagccatatc aagaaacggc gctttggtca ttgcgcccgc attattcacc
    13201 agcacatcaa tacgccccag ccgttgaatg agtttctcca gcgcctgcgc accttctggc
    13261 agtttaccga gctccagctg cacgatctcc gcacgtacgc cgtggctaac tacctcacgc
    13321 gcggtatctt ttgccccttc ttcatctgag tgccaggtaa tgccaatatc aaacccctgc
    13381 tgcgccagta ataacgcgca ctctttgccg atccccgaat cggaggcggt aataatcgca
    13441 acctgtgcca tcaagttctc cacttaacgc tgaataaacg ttaagtatag aaggcgcata
    13501 tcatcagcgt ttgtaacccc cgcccaacgc accagtgagt tgaatagaag catccagcca
    13561 ctgcccttgc aataacaggc cattggcccg ctcacgcagc gcggggattc tggcttcgct
    13621 gacgcgggaa ccaacaatga tgcccgcgtt aaagcgcgcc tgcgcaagac ctaccacacg
    13681 caaggcatcg cgctcaattt gcgcctgatg ctggtttttc tccgcaagtg tctgaacctg
    13741 actggctgcc cgcgccacgt cattcaccgc ttcaaccacc gctttgttgt agctggcgat
    13801 agacaagttg ctttcggctt ttgcgatatt gagattggcg ttaagacgac cactatcgaa
    13861 aatgggtagc gtcaggcctg ccgtaacgcc catttgctgc gcggaatgac ggaacagatc
    13921 gcttaagtgc aacgcatcct gttgcaggaa ggccatcagg ttgatgtcag gataaaatgc
    13981 cgcttttgcc gcatcaatgg tgcttagcga tgactcaacg taccagtgcg ccgcctgcaa
    14041 atctgcccgc cgggccagta aggagtaccc cagttcatca ggaagctggc ttgccacttt
    14101 cggcaacgcg accggtttaa gcttcaatga ctttgtctgg ttatttgtaa gtgcgcttaa
    14161 ccgtgcctca ataattttca ttttccccgc gacatcgttg agctgctgcc gggttttgct
    14221 ggcattaata tcggtttcca caccttcaac tgaagaagta atcccgttct gatatagctg
    14281 gcgatcggtc gcgataatgg tgttctgctc tttttctatt tgctgcaaga ccgtgtttaa
    14341 cgccgcctgg gtttgccact cccagtacag gcgggctacg ctgccagcca gcaattggcg
    14401 ggtttgctcg cgttccgccg cccgtgcttt aaccgtaccc aggcgggcag taacctccgc
    14461 ccgattcttt ccccagatat cgagatgcca gcccgccgtt aagccaaaag taccgttggt
    14521 gtaccacggg ccggtcgtac ctgcggccgg atcgttcaga gcaaacggcc ccattaagcc
    14581 ttctgccgac attttttgcc gctccatatc cgccgaaaag tcgatctgcg gaccatcctg
    14641 agtggcaact gccttcgcct gggcttcagc tagctgaatg cgctgttcag ccacctgcat
    14701 atccggtgcg ttctgtagtg cattgttaat taatgaagtg agctgattat cgtgatactc
    14761 cagccaccat tggctgtctg gccaaccatt tttcagcgcc gtgggtaatg cggtgtcaac
    14821 ttgtgcagcg ggcgtttgct ggcttaacgc ctggcgggtt tcatgcatag gcgcacaccc
    14881 ggccagcatc agtaacagcg gaaaacaggc gatggctgga taaaaggaat cacgattcat
    14941 gggggaataa tcaggtaaga aacggtgcgc ggagattacc gtgtgttgcg atatattttt
    15001 tagtttcgcg tggcaataca tcagtagcaa taaaacgaca tacccagaaa aatatagact
    15061 aagtaaatga tatcttccga tttatcttaa tcgtttatgg ataacggcaa agggcttcgt
    15121 tttttcctat acttattcag cactcacaaa taaaggaacg ccaatgaaaa ttatactctg
    15181 ggctgtattg attattttcc tgattgggct actggtggtg actggcgtat ttaagatgat
    15241 attttaaaat taattaatgt catcaggtcc gaaaataacg agaatatttc agtctctcat
    15301 cctgttgcgc tcctgtcatg tgcattgctt catataatca ctggcgcaag gagcgcgcag
    15361 ggggcggcca atcgccgccg
//
LOCUS       DAZPJR010000095        15379 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPJR010000095 DAZPJR010000000
VERSION     DAZPJR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.096"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            100..2634
                     /gene="mrcB"
                     /locus_tag="ACHVB7_002732"
     CDS             100..2634
                     /gene="mrcB"
                     /locus_tag="ACHVB7_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR1914674.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2854..5043
                     /gene="fhuA"
                     /locus_tag="ACHVB7_002733"
     CDS             2854..5043
                     /gene="fhuA"
                     /locus_tag="ACHVB7_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR1914675.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            5094..5891
                     /gene="fhuC"
                     /locus_tag="ACHVB7_002734"
     CDS             5094..5891
                     /gene="fhuC"
                     /locus_tag="ACHVB7_002734"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR1914676.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5891..6781
                     /gene="fhuD"
                     /locus_tag="ACHVB7_002735"
     CDS             5891..6781
                     /gene="fhuD"
                     /locus_tag="ACHVB7_002735"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR1914677.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6778..8760
                     /gene="fhuB"
                     /locus_tag="ACHVB7_002736"
     CDS             6778..8760
                     /gene="fhuB"
                     /locus_tag="ACHVB7_002736"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR1914678.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8918..10198)
                     /gene="hemL"
                     /locus_tag="ACHVB7_002737"
     CDS             complement(8918..10198)
                     /gene="hemL"
                     /locus_tag="ACHVB7_002737"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR1914679.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10364..10411
                     /gene="yadX"
                     /locus_tag="ACHVB7_002738"
     CDS             10364..10411
                     /gene="yadX"
                     /locus_tag="ACHVB7_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR1914680.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10423..11844
                     /gene="clcA"
                     /locus_tag="ACHVB7_002739"
     CDS             10423..11844
                     /gene="clcA"
                     /locus_tag="ACHVB7_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR1914681.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11868..11933
                     /gene="yadW"
                     /locus_tag="ACHVB7_002740"
     CDS             11868..11933
                     /gene="yadW"
                     /locus_tag="ACHVB7_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR1914682.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11926..12270
                     /gene="erpA"
                     /locus_tag="ACHVB7_002741"
     CDS             11926..12270
                     /gene="erpA"
                     /locus_tag="ACHVB7_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR1914683.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12317..12940)
                     /locus_tag="ACHVB7_002742"
     CDS             complement(12317..12940)
                     /locus_tag="ACHVB7_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR1914684.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12978..13778)
                     /gene="btuF"
                     /locus_tag="ACHVB7_002743"
     CDS             complement(12978..13778)
                     /gene="btuF"
                     /locus_tag="ACHVB7_002743"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR1914685.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13771..14469)
                     /gene="mtnN"
                     /locus_tag="ACHVB7_002744"
     CDS             complement(13771..14469)
                     /gene="mtnN"
                     /locus_tag="ACHVB7_002744"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR1914686.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14553..>15379
                     /gene="dgt"
                     /locus_tag="ACHVB7_002745"
     CDS             14553..>15379
                     /gene="dgt"
                     /locus_tag="ACHVB7_002745"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1914687.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT         3477 a         3867 c         4292 g         3743 t
ORIGIN      
        1 ggaccgatcg agcacaaatt ttgagagata tcttcttctg tcttgtaaca gaagaacaga
       61 aaatcgggct tttgcgcctg aatattgcgg agaaaaagca tggccgggaa tgaccgcgag
      121 ccaattggac gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag ccgtcgtcgt
      181 tacgaagatg acgatgatta cgacgattat gatgactatg aggatgaaga accgatgccg
      241 cgcaaaggta agggcaaagg caaagggcgt aagcctcgtg gcaaacgcgg ctggctatgg
      301 ctgctgttaa aactggctat cgtttttgcc gtgctgatcg ccatttacgg cgtttatctc
      361 gatcaaaaaa ttcgtagccg tattgatggc aaggtctggc aactgcccgc ggcagtttat
      421 ggccgaatgg tcaaccttga accagatatg accatcagca agaacgaaat ggtgaagctg
      481 ctggaggcaa cacagtatcg tcaggtgtcg aaaatgaccc gtcctggcga atttaccgtg
      541 caggccaaca gcattgagat gatccgccgt ccgtttgatt tcccggacag caaagaaggg
      601 caggtgcgtg cgcgtctgac ctttgatggc gatcatctgg cgacgatcgt caatatggag
      661 aacaaccgtc agttcggttt cttccgtctt gatccgcgcc tgatcaccat gatctcttcg
      721 ccaaacggtg agcagcgtct gtttgtgccg cgcagtggtt tcccggactt gctggtggat
      781 actttgctgg cgacagaaga ccgtcatttc tacgagcatg atgggatcag tctctactcc
      841 atcggacgtg cggtgctggc aaacctgacc gccggacgca cggtgcaggg agcgagtacg
      901 ctgacgcaac agctggtgaa aaacctgttc ctctccagcg agcgttctta ctggcgtaaa
      961 gcgaacgaag cttacatggc gctgatcatg gacgcgcgtt acagcaaaga ccgtattctt
     1021 gagctgtata tgaacgaggt gtatctcggt cagagcggcg acaacgaaat ccgcggcttc
     1081 ccgcttgcga gcttgtatta ctttggtcgc ccggtagaag agctcagcct cgaccagcag
     1141 gcgctgttag tcggtatggt gaaaggggcg tccatctaca acccgtggcg taacccgaaa
     1201 ctggcgctgg agcgacgtaa tctggtgctg cgtttgctac aacaacagca gattatcgat
     1261 ggtgaactgt atgaaatgct cagcgcgcgt cctctgggcg tacagccgcg cggtggggta
     1321 atctctccac agccagcctt tatgcaactg gtgcgtcagg agctgcaggc gaaattaggc
     1381 gataaggtaa aagatctctc cggcgtgaag atcttcacta cctttgactc ggtggcccag
     1441 gacgcggcag aaaaagccgc cgtggaaggc attccggcac tgaagaaaca gcgtaagttg
     1501 agcgatcttg aaactgcaat tgtggtcgtc gaccgcttta gtggtgaagt tcgtgcgatg
     1561 gtgggaggtt ctgagccgca gtttgcgggc tacaaccgtg cgatgcaggc gcgtcgttcg
     1621 attggttccc ttgcaaaacc agcgacttat ctgacggctt taagccagcc gaaaatctat
     1681 cgcctgaaca cgtggattgc cgatgcgcca atcgcgctgc gtcagccgaa tggccaggtc
     1741 tggtcaccgc agaacgatga ccgtcgttat agcgaaagcg gcagagtgat gctggtggat
     1801 gcgttgaccc gttcgatgaa cgtgccgacg gtaaatctgg ggatggcgct ggggctgcct
     1861 gcagttacgg agacctggat caaactgggc gtgccgaaag atcagctgca tccggttccg
     1921 gcgatgctgc tgggggcgtt gaacttaacg ccaatcgaag tggcgcaggc attccagacc
     1981 atcgccagcg gcggtaaccg tgcaccgctt tctgcactgc gttcggtaat cgcggaagat
     2041 ggcaaggtgc tgtatcagag cttcccgcag gcagaacgcg ctgttccggc gcaggcggcg
     2101 tatctgacac tatggaccat gcagcaggtg gtacaacgtg gtacgggtcg tcaacttggg
     2161 gcgaaatacc cgaacctgca tctggcaggg aaaacaggga ctaccaacaa taacgtagat
     2221 acctggtttg cgggcattga cggcagcacg gtgaccatca cctgggtagg ccgtgataac
     2281 aaccagccga ccaaactgta tggtgccagc ggggcaatgt ctatttatca gcgttatctg
     2341 gctaaccaga cgccaacgcc gctgaatctt gttccgccag aagatattgc agatatgggc
     2401 gtggactacg acggcaactt tgtttgcagc ggtggcatgc gtgtcttgcc ggtctggacc
     2461 agcgatccgc aatcgctgtg ccagcagagc gagatgcagc agcagccgtc aggcaatccg
     2521 tttgatcagt cttctcagcc gcagcaacag ccgcaacagc aacctgctca gcaagagcag
     2581 aaagacagcg acggtgtagc cggttggatc aaggatatgt ttggtagtaa ttaacatcta
     2641 agcgtgaaat accggatggc gaattgccat ccggtaaaat aacatcccac ctaaagtatt
     2701 aaccctttct tttcatctgg ttgtttatta acccttcagg aacgctcaga ttgcgtaccg
     2761 cttgcgaacc cgccagcgtt tcgaatatta tcttatcttt ataataatca ttctcgttta
     2821 cgttatcatt cactttacat cagagatata ccaatggcgc gttccaaaac tgctcagcca
     2881 aaacactcac tgcgtaaaat cgcagttgta gtagccacag cggttagcgg catgtctgtt
     2941 tatgcacagg cagcggttga accgaaagaa gacactatca ccgttaccgc tgcacctgcg
     3001 ccgcaagaaa gcgcatgggg gcctgctgca actattgcgg cgaagcactc tgctactgcg
     3061 actaaaacgg atacaccaat tgaaaaaacg ccgcagtcta tttcggttgt gacaaatgaa
     3121 gagatgcaga tgcgtcaatt tcagtctgta aaagaagcat taggttatac acctggcgtt
     3181 acggttagca gtcgtggtgc ttctaataca tatgattttg ttatcattcg tggtttctca
     3241 tctgttggtc tgaatcaaaa taattacctt gatggactaa aacttcaggg caacttttat
     3301 aacgatgctg tgattgatcc ttacatgctc gagagggttg aactgatgcg tgggccgacg
     3361 tctgttctct atggaaaaag taatcctggt gggattattt ctatggtgag taagcgtccg
     3421 accactgaac ccctgaaaga aattcagttt aaaatgggga cggataatct gtttcagacc
     3481 ggattcgatt tcagtgatgc actggatgat aacggtgagt tctcttaccg tttgacgggc
     3541 ctcgctcgct caacaaacga acagcagaaa aactctgaat ctcagcgtta taccattgct
     3601 ccatcattct catggcgtcc agacgacaaa accaatttta ctttcctgtc ctatttccag
     3661 aatgaaccag aaacgggtta ttacggttgg ttgccgaaag aggggaccgt tgagccattg
     3721 cctaatggta agcgtctacc gactgacttc aatgaaggtg catcgaataa tacatactcc
     3781 cgtaaccaga aaatggtggg atatagtttt gaacatggtt ttaatgacac cttcaccgtg
     3841 cgtcagaatc tgcgtttcag tgaaatgaaa acctcacaga aaagtgttta tggcacaggg
     3901 attgctggtg atggtcatac cctaaaccgc gggacagtgg tggataatga gcgtctacaa
     3961 aactttagcg ttgataccca acttgaaagt aagtttgcta caggtgacgt cgagcatact
     4021 ttgctgacag gggtagactt catgcggatg cgtaatgata tcaatgccag ctttggatcc
     4081 gcaccatcca tcgatcttta taacaaatat caccctgaat actttgcatt tggtagcgca
     4141 gagccatacc agatgaatga aagcaaacaa acaggtattt atgttcagga tcaggcggaa
     4201 tggaataaat gggtattcac tctgggggga cgctacgatt ggtctaagca agcgaccact
     4261 gttcgtgaaa actcttatac gccgactgaa ggttatattg agcgcaatga tcatcagttc
     4321 acctggcgcg gtggtgtaaa ttacttattc gataatggta tttcacctta ctttagctat
     4381 agccagtcct ttgaaccgag tgctttcgat ctgtggagca acccgcgcat ttcctataag
     4441 ccatcgaaag gtgaacagta tgaagctggc gtaaaatatg ttccgaatga tatgccggtc
     4501 gttgttacgg gcgcagtcta tcaattgacg aaaacaaata acctgacagc agacccaaca
     4561 aacccattag cgcaagtccc agcaggtgag attcgcgctc gtggtgtgga acttgaagca
     4621 aaagcggcgt taaatgccaa tattaacttg acggcttctt atacctacac cgatgcggaa
     4681 tacaccaaag acaccaatct caaaggtaaa actccagaac aagtaccgga gcatatggca
     4741 tctctctggg gggattatac cttcaatgaa gggccgcttt ctggtttaac attgggaaca
     4801 ggtggtcgtt ttattggttc cagctatggt gatccggcaa acacttttaa agtgggtagc
     4861 acagctgtaa tggatgctgt tgtaaaatat gatctggcac gctttggtat ggcgggatcc
     4921 agccttgctg tgaacgtcaa caatttgctc gatcgtgagt atgttgccag ttgcttccag
     4981 acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac cttccgtttc
     5041 taatttctct tttgggacac ggatttccgt gcccatttca caagttggct gttatgcagg
     5101 aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg
     5161 ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga
     5221 ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt catcagcctc
     5281 cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt
     5341 ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc
     5401 gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggtgcgg
     5461 cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc
     5521 ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg
     5581 cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc ggcgctggat atcgcccacc
     5641 aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg acggtcattg
     5701 ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgca
     5761 gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa
     5821 tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt
     5881 ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc
     5941 gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg accccaatcg
     6001 tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta
     6061 cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc
     6121 agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg aaatgaaacc
     6181 gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg cacgtattgc
     6241 gccgggtcgc ggatttaact tcagtgacgg caaacagccg ttggcgatgg cgcgtaaatc
     6301 gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc atttagcgca
     6361 atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt
     6421 actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt
     6481 ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg
     6541 gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg
     6601 ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc
     6661 catgccgttt gtccgcgccg gacgctttca gcgcgtacct gcagtctggt tttatggtgc
     6721 gacactctcg gcaatgcact ttgtgcgcgt tctggataac gccatcggag gtaaagcgtg
     6781 agtaaacgaa ttgcgctttt tccggcgtta ttgctggcgc tgttagtgat tgtcgctacg
     6841 gcgctcacct ggatgaactt ctcgcaggcg ctgccgcgta gccagtgggc gcaggctgcc
     6901 tggtcgccag acatcgatgt catcgagcag atgatttttc actacagctt gttgccgcgt
     6961 ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct gtttcagcaa
     7021 gtgctgcgta acccgctggc ggagccgacg acgcttggcg ttgctacagg cgcgcaactg
     7081 ggaattaccg tcaccacact ttgggcgatc ccaggtgcta tggcgagcca gtttgccgca
     7141 ctggtgggcg cttgtgtcgt tggcttaatc gtctttggcg tcgcgtgggg gaaacggctt
     7201 tcgccggtta cgcttattct cgcggggctg gtggtgagcc tttattgcgg ggcaattaat
     7261 cagctactgg ttatcttcca tcacgaccag ctgcaaagca tgttcctgtg gagcaccgga
     7321 acgctgacgc aaaccgactg gggcggcgtt gagcgtttat ggccgcagtt gctgggcggc
     7381 gtgatgttga cgttattgct acttcgcccg ttaaccctga tggggcttga tgatggcgtg
     7441 gcgcgcaatc tcgggctggc cttgtcgctc gcgcgtctgg cggcgctgtc gctggcgata
     7501 gtcatcagtg cgctgctggt gaatgcggtg gggattatcg gctttatcgg tttgttcgcg
     7561 ccgctgctgg cgaaaatgct gggggcgcgg cgtttgttgc cacggttgat gctggcgtcg
     7621 ctgattgggg cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg
     7681 tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg
     7741 ctgttgccgc gtttacgcag cattagcgcg ccggatatga aggtcaacga tcgtgtcgcg
     7801 gctgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgct gttgatggct
     7861 gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg
     7921 ttgctcgagg atttgatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc
     7981 gtcatgctgg cggtggcggg ctgtattatt cagcggctga ccggaaaccc gatggcaagc
     8041 ccggaagtgc tggggattag ctctggcgcg gcgtttggcg tggtgttaat gctgtttctg
     8101 gtgccgggta atgcctttgg ctggctgtta cccgcgggca gtatcggggc ggcggtgacg
     8161 ctgttgatca ttatgatcgc cgccggccgc ggtggatttt ccccacaccg tatgttactg
     8221 gcggggatgg cgttaagtac cgcattcacc atgcttttga tgatgttgca ggcgagcggt
     8281 gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa cgcgaccgat
     8341 gcgcaggtct ggcgtaccgg aattgtgatg gtgattttgc tggcgattac cccgctgtac
     8401 cgccgctggc tgaccatttt accgctgggt ggtgataccg cacgagccgt aggaatggcg
     8461 ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg
     8521 atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg
     8581 ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg tttactgctg
     8641 gtattcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg
     8701 ctgtcgacct ttatcggcgc gccatatttt atctatttgc tgagaaagca gagccgttaa
     8761 tttttccata taacggccct gtacgcctgg acggataaag cgtttacgcg acaacggatg
     8821 cccgatgcga cgctggcgcg tcttatcggg cctacaaacg gccccgaatc gtaggtcgga
     8881 taaggcgttc acgccgcatc cgacaaacca tgctggatca caacttcgca aacacccgac
     8941 gtgcagcatc gatggtgtta ttgatatctt ccatgctgtg cgccacggac ataaagcccg
     9001 cttcaaacgc tgacggtgcc aggtaaacac cttcgtccag catcatatgg aagaaacgct
     9061 taaagcgttc cacgtcacag gccatcacat cctgatagca cgtcacggac tcggcgtcgg
     9121 taaagaaaat accgaacatg ccgccaacgt ggttaacgac cagcggaatt ccggcttctt
     9181 ctgccgcttc cagcagacct tctgccagac gtgttgtcag ctcatccaac gtttcgtgaa
     9241 cgcccggttg cgcgacttca ttcagacagg cgaaaccggc tgccatcgca attgggttac
     9301 cggaaagcgt acccgcctga tagaccggac ccgtcggggc cagcgcatcc attacatcac
     9361 gacgaccacc gaatgcgcct accggcattc caccgccgat gattttgccc aggcaggtga
     9421 gatccggttc cacaccgtaa taatcctgtg cgccagccag tgccacgcgg aagccggtca
     9481 ttacttcatc gatgatcagc aatgcgccaa attcgtcgca cagcgcacgc agacctggca
     9541 ggaagtctgg cagcggtgga acacagttca tattgcctgc caccggctcg atgataatac
     9601 aggcaatctc ttgcgggtat tgctcaaacg cggcgcgtac agaagccaga tcgttataag
     9661 tacaggttaa ggtatgtttg gcgaaatctg ccggaacgcc cggcgagttt ggctggccta
     9721 acgtgagtgc gccagaaccg gctttcacca gcaggcagtc agcgtgaccg tggtaacacc
     9781 cttcaaattt aatgattttg tcgcgaccgg taaaaccacg ggccagacgg atggcgctca
     9841 tcgtcgcctc ggtgccggag ttcaccatgc gcaccatatc catggtcggg accaattcgg
     9901 tcaccagttg cgccattttc acttccattt cggttggtgc accaaagctt aaaccacgct
     9961 cggcggcttc aatcacggca ttgcggattg ccggatggtt atggcccagc accatcggcc
    10021 cccaggaacc gacataatcg atataggctt tgccatcaac atcgtacaga taagcgccgt
    10081 ccgctttttc gataaacagt ggagtgccgc ccacgccagt aaaggcgcga acaggggagt
    10141 tcacaccgcc agggatcagc tcgcgcgctg cgctgtaaag attttcagac ttactcatgg
    10201 agggttcctg attcgtagaa aaagtgaatg gctgctattc tatgttattc ataacaagtt
    10261 aaatactcgt caaacatcag gctgcttgta ctggtgcaat cctgatttct tagagtataa
    10321 aagttttgtg catttgaaac attacgcttt gcaaaggatt ttcatggaac gtgcgagtaa
    10381 aatgccgtca tcttatttgt atgaccaata agtgatcatt ggatgaaaac tgatactccc
    10441 tctttagaaa caccgcaggc cgcgcgcctg cgacgcagac aactgattcg ccaacttctt
    10501 gagcgcgata aaaccccgtt agccattttg tttatggcgg cagtcgtcgg cacgcttgtt
    10561 gggctggcag cggttgcttt tgacaaaggt gtcgcctggt tgcagaacca acgtatgggg
    10621 gcgctggtac atactgctga taattatccg cttctgttaa ccgtcgcttt tctctgttcg
    10681 gcggtgctgg cgatgtttgg ctactttttg gtgcgcaaat acgcgccgga agcaggtggt
    10741 tcggggatcc cggaaattga aggggcgctg gaagatcaac gtcccgttcg ctggtggcgt
    10801 gtattgccgg tgaagttctt tggcgggctg gggacactcg gcggaggcat ggtgttgggg
    10861 cgcgaagggc caaccgtgca gatcggcggt aacattggcc gtatggtgct tgatattttc
    10921 cgcctgaaag gtgacgaagc tcgccatacg ctgctggcaa ccggtgctgc tgcggggctg
    10981 gctgcggcct ttaacgcgcc gctggcgggt attttgttta ttatcgaaga gatgcgtccg
    11041 cagtttcgct atacgttaat ttcgataaaa gcggtattta ttggcgtcat tatgtcgacc
    11101 attatgtacc ggatttttaa tcatgaagtt gcgttgattg acgtcggtaa actttctgat
    11161 gcgccgctta atacgctgtg gctttatctg atcctcggta ttatttttgg cattttcggc
    11221 cctattttta ataaatgggt gctggggatg caggatttgc tgcaccgtgt gcacggcggc
    11281 aatattacca aatgggtact gatgggcggt gcgattggcg gtctgtgtgg attgctgggg
    11341 tttgtggcac cagcaacgtc gggcggcggt tttaacctga ttcctatcgc taccgcgggg
    11401 aatttcagca tgggaatgct ggtgtttatc ttcgtcgctc gggtcattac caccttactc
    11461 tgcttctctt ccggcgcgcc gggcggtatt tttgccccga tgctggcgct gggtactgtg
    11521 ctgggaaccg ctttcggaat ggttgccgtt gagctgtttc cgcaatatca ccttgaggcg
    11581 gggacgtttg ctattgccgg aatgggggca ttactggcgg catctattcg cgcgccgtta
    11641 acggggatca ttctggttct ggagatgacc gataactacc agctcatttt gccaatgatt
    11701 attaccggtc ttggcgcaac actattagcg caatttaccg gcgggaaacc gctatactcg
    11761 gcgattcttg cgcgcacgct ggcaaaacag gaagctgagc aactggcgcg aagcaaggcc
    11821 gcatcagcca gcgagaatac ttgaacgaaa taccagggta ttagataatg gcgattatta
    11881 ttgggttaga atttgcccaa ttgccgatgt cgtttggagc aaaatatgag tgatgacgta
    11941 gcactgccgc tggagtttac cgacgcagca gccaacaaag ttaaaagcct gatcgctgac
    12001 gaagataacc cgaatctgaa attacgcgtg tatatcaccg gtggcggttg cagcggcttc
    12061 cagtatggtt tcacctttga tgatcaggtg aacgaaggcg atatgaccat cgaaaaacag
    12121 ggcgttggct tggtggttga tccgatgagc ctgcaatatc tggtcggcgg ttccgttgac
    12181 tataccgaag gactggaagg ttctcgtttc atcgtgacca acccgaacgc gaaaagcacc
    12241 tgcggttgcg gttcttcctt tagcatctaa tctgttgttc atgtgccgga taaactatcc
    12301 ggcgcatctt ctctcttcaa cgcccattct catccagcgc aaacgtcggt agcttaagat
    12361 gccaacgaat agccgccagc cgaatcaata gcgtcacgac catgcccatc atactggctg
    12421 tttccagtgg tacggaaaat gtgtaataag ccgtagcgtg gacaataccg ccgataatac
    12481 aggcagttgc gtagatttct gtacgtaaaa tcatggggat ctcgcgggcc agtacatcac
    12541 gaatgatccc gccgccaacg ccagtaatga cgaccataca aaccgcgatt aacggaccga
    12601 cttccgcatt aaaggcttta ttcacgccaa tgccgacaaa caccgccaga ccaacggcgt
    12661 ccagcaccgg caacatccat tttggtaagc gtcttggctg gcgcaccagc acgatggtca
    12721 gcatgctggt taccattgca acgaccagat cggtgggatc tttcacccaa aataccgggc
    12781 cgtgatccag cgccatgtcg cgaattgtcc cgccgcctac agcggtaacc acgcccagta
    12841 ccagaacacc aaaagggtcc atacgcaatt ttccggctaa caaaacgccg gagatggcaa
    12901 ataccgctgt gccgactata tccagccaat agacgagcat tgttcaatcc ccactgagca
    12961 cctaaaaagg tcaggcgcta atctacctgt gaaagcgcat tacagagctg ttgtgcagcg
    13021 aggataatac gtgggcttgc acgttcaaac cagtcactcg tgagaggaat aacgggaatt
    13081 ttgagctgtt caccccagta ttgtttgatt ttaggaattt ggtccggtcc gcctgtaatg
    13141 acaatcgcct gtggcgagcg tgctaacacc tgttcgcggc taacttgcgg ccagggaacc
    13201 cggctgtctt taaagatgtt ttctccgcca caaacttcga gtacctggtt ttgaatcgac
    13261 tcttttccac tggtaaatgg cggattaatg ccgaattgca gaaaaacacg ttttttaggt
    13321 ttatcagcat attgcgcttt caattgcgcg tactgatcca gcagggattg cgcggcttgt
    13381 tcggccttat ccggttgcgg actccagggg gccaattgac gtaacgcatt ggcaatttgt
    13441 tcaatgcttg tcgcatcgac ccacatcact tttattccca gcgaagccag ctggtcaacc
    13501 tgccgctcgg cattacctcc acgccaggca atcaccagat cgggtttcag cgcgacaatg
    13561 cgttccagat tcatcccctg ccaggtggaa acctgctcaa tcttttgcgc ttgtggagga
    13621 tagtcggaat agctgctgac cccaaccggc gtgatcccgg cggcaaaggc aagttcagtg
    13681 ttggcgggag aaagcgtgat gacgcgcggc gcggcgttga gccacagtgg cgcaagaaaa
    13741 gacagggcga ccagcgccct gaacagtgac ttagccatgt gccagtttct gcaccagtga
    13801 ctcaaccatc aggctggact gtttagcggc aacagccagg aactcatcga agctaagatg
    13861 agactgttga tcggccacgt cggagatggc gcgtacgaca acaaacggga cgttgaaatt
    13921 gtggcagaca tgggcgattg ccgtcgcttc catctctaca gcaatggcct gtgggaagtt
    13981 gtggcggatt ttcgccagac caacagaacc gttgatgaaa gcgtcgccgc taacaatcag
    14041 gccacgtaca gcgttaagat tcagttcggc aatgcaggcc tcagcggcag cgatcagttt
    14101 atcgtcagct ttaaagcctg ccggacagcc tggtaactga ccgtattcat aaccaaatgc
    14161 cgtgacatcc gcgtcgtgat aacgcgcttc gtccgagaca acgatatcgc ccactttcag
    14221 cgttggtgcc aggccaccgg cagaaccggt gttaataatc acatctggct tgcagtgttc
    14281 caacagcaga gtggcaccca gcgccgcagc gactttaccg atgcccgatt tcagaagcgc
    14341 aacctcggtt ccattcagtt ggccggtata gatttcgcaa ccgccgagac tgatagtttg
    14401 acggttttcg attttgtcac gcagcagcgt aacttcttct tccattgcac caatgatgcc
    14461 gattttcata gatttactcg cgataagccc gatttgaaag catagtttac catgcgctta
    14521 cggggaagcg tatttctcac gcgggagagg acatggcaca gattgatttc cgaaaaaaaa
    14581 taaactggca tcgtcgttac cgttcaccgc agggcgttaa aaccgaacat gagatcctgc
    14641 ggatcttcga gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt cgtctgcaac
    14701 aaaagaccca ggtttttcca ctggagcgca atgccgccgt gcgcacgcgt cttacccact
    14761 cgatggaagt ccagcaggtg ggacgctaca tcgccaaaga aattttaagc cgtctgaaag
    14821 agcttaaatt gctggaagca tacggcctgg atgaactgac cggccccttt gaaagcattg
    14881 ttgagatgtc atgcctgatg cacgatatcg gcaatccgcc gtttggtcat tttggcgaag
    14941 cagcgataaa tgactggttt cgccaacgtt tgcacccgga agatgccgaa agccagcctc
    15001 tgactgacga tcgctgcagc gtggcggcac tacgtttacg ggacggggaa gaacagctta
    15061 acgagctgcg gcgcaagatt cgtcaggact tatgtcattt tgaggggaat gcacaaggca
    15121 ttcgtctggt gcatacattg atgcggatga atctcacttg ggcacaggtt ggcggtattt
    15181 taaaatatac ccgtccggcg tggtggcgtg gcgaaacgcc tgagacacat cactatttaa
    15241 tgaaaaagcc gggttattat ctttctgaag aagcctatat tgcccggttg cgtaaagaac
    15301 ttaatttggc gctttacagt cgttttccat taacgtggat tatggaagct gccgacgaca
    15361 tctcctattg tgtggcaga
//
LOCUS       DAZPJR010000096        15337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPJR010000096 DAZPJR010000000
VERSION     DAZPJR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.097"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            99..851
                     /locus_tag="ACHVB7_002746"
                     /pseudo
     CDS             99..851
                     /locus_tag="ACHVB7_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
     gene            complement(892..1824)
                     /locus_tag="ACHVB7_002747"
     CDS             complement(892..1824)
                     /locus_tag="ACHVB7_002747"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1914688.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2188..4974
                     /gene="polA"
                     /locus_tag="ACHVB7_002748"
     CDS             2188..4974
                     /gene="polA"
                     /locus_tag="ACHVB7_002748"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR1914689.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5356..5988)
                     /gene="yihA"
                     /locus_tag="ACHVB7_002749"
     CDS             complement(5356..5988)
                     /gene="yihA"
                     /locus_tag="ACHVB7_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR1914690.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6285..6451
                     /locus_tag="ACHVB7_002750"
                     /pseudo
     CDS             6285..6451
                     /locus_tag="ACHVB7_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6570..7079
                     /gene="yihI"
                     /locus_tag="ACHVB7_002751"
     CDS             6570..7079
                     /gene="yihI"
                     /locus_tag="ACHVB7_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR1914691.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7268..8641
                     /gene="hemN"
                     /locus_tag="ACHVB7_002752"
     CDS             7268..8641
                     /gene="hemN"
                     /locus_tag="ACHVB7_002752"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR1914692.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8831..8941)
                     /locus_tag="ACHVB7_002753"
     CDS             complement(8831..8941)
                     /locus_tag="ACHVB7_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR1914693.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="ACHVB7_002754"
     CDS             complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="ACHVB7_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR1914694.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="ACHVB7_002755"
     CDS             complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="ACHVB7_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR1914695.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="ACHVB7_002756"
     CDS             complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="ACHVB7_002756"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR1914696.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13479..15302
                     /gene="typA"
                     /locus_tag="ACHVB7_002757"
     CDS             13479..15302
                     /gene="typA"
                     /locus_tag="ACHVB7_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR1914697.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         4004 a         3829 c         3937 g         3567 t
ORIGIN      
        1 cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca cctcaaaaac
       61 accatcatac actaaatcag taagttggca gcatcaccag cgagtcgaaa tttaatcaga
      121 ttaacattat caatcacggg gaagacgttg cccaaatcga tgctttcgtt gcaaaaacca
      181 tgctggatcg cgttaaagac aaagattata tcaatgtcaa tctgacctac gaacttgata
      241 agttaacaaa agggaatcag caactcggta gtggtgagtg gtcattgatt gctgaatcta
      301 ttgatccctc agcagtgcgc caatttatca tccagtataa cattgctatg cagaagcagc
      361 ttgctgtaca ccctgagtta gcaaacgatg aagttgctct gcaagaagtg aatgctgcat
      421 tgttcaaaga gtatttaccg ttattacaaa aaagtgagcc gaccattaaa caaccggtaa
      481 gatggaagaa cgcactcggc gaactaaatg ccaatctgga tatcagtatt gccgacccag
      541 ccaaatcttc atcatccaca aacaaagata tcaaattgct caattttgat gtgaagttac
      601 cgcttaatgt cgtcacagaa accgcaaaac agcttaattt atctgaagga atggatgcgg
      661 aaaaagcgca aaagcaggct gataaacaaa tcagcgggat gatgacattg ggtcagatgt
      721 ttcagttaat cacgattgac aacaataccg cctcgctgca gctgcgttat acaccgggta
      781 aagttgtgtt taacggacag gagatgagcg aagaagaatt tatgtctcgt gccggacgtt
      841 ttgttcatta aaatactcac tcgcctctgc tcattcagag gcgagtaaaa cttacttatt
      901 ctgacgctgt gatgacatga gagacgtcag aagccggtct ttttcctgcc acaatgagtt
      961 cagccattgc tgaaaatggc gcttaaagct tttgtcattg atgtagtcgc catgtaactc
     1021 atcggcaata ggctgtaaat ccacatgtac gacaatccgc gtcagtttac cgcttaacat
     1081 atcgaagaac ggctgacggt tattgtccgg ataacacagc gtaacattaa gcagtttatc
     1141 gaattgttta cccagtacat ttagcgccat cgcaatgcct gccgcctttg gtggcaacaa
     1201 gttttgaaaa gtggagtggg tttgctgatg tttttcttgc gtgaaacggg agccttcaac
     1261 gaaattgaca atggtggtgg gatgcaggcg aaacttttca caagaacgcc gagtggtttc
     1321 aacatcttta ccacgtcgct ccggatgacg taataaataa gcgcgggaat aacgcttcat
     1381 aaacggcata tccagcgccc agcacgccag gccaagaaat ggcacccagg ccagctgttg
     1441 cttgaggaaa tatttattca tcggaatgtg cttacgaaac agcacgcaca gtacgacaat
     1501 atctgcccaa ctacggtggt tacaaataag cagataccag ttcttcttac ttaacccttc
     1561 cagcccgtga acttcccatt gcaggtgtgg gttcagatgc agtaataccg ccagaccttc
     1621 acaccagcaa tacatcataa aatcacaaaa acgcgaaacc tttcgccaaa tgactggcac
     1681 aggtagcaat agttttacaa tcccggcaat gatgatcggg acggaacaaa aaatagtgac
     1741 caaaatggtc aatacgatac tcagcaaaag ggttatcgca gcgagtattc tcgtcataat
     1801 gaatttattc aaaaggttag ccatagacag tgcgctaaag aagcgcaagg cgcagatttt
     1861 agcagaaaac gacccaaata acggatgatc cttaaggaga aaaataatcc atatctatcc
     1921 acattagaaa aaatcccatt atctcaatta ttagggatgg ctttattttt aactgcatga
     1981 aaaacaaaga caaacatcat gctgtaaaaa gcatgataat aaattaaaag cgatgtaaat
     2041 aatttatgca caaagttatc cacatgacga tttgcgagcg atccagaaga tctacaaaag
     2101 attttcacga aaagcggtga aaaactcatg ttttcatcct gtctgtggca tcctttaccc
     2161 ataatctgat aaacaggcac ggacattatg gttcagatcc cccaaaatcc acttatcctt
     2221 gtagatggtt catcttatct ttatcgcgca tatcacgcgt ttcccccgct gactaacagc
     2281 gcaggcgagc cgaccggtgc gatgtatggt gtcctcaaca tgctgcgcag tctgatcatg
     2341 caatataaac cgacgcatgc agcggtggtc tttgacgcca agggaaaaac ctttcgtgat
     2401 gaactgtttg aacattacaa atcacatcgc ccgccaatgc cggacgatct gcgagcacaa
     2461 atcgaacccc tgcacgcgat ggttaaagcg atgggactgc cgctgctggc ggtttctggc
     2521 gtagaagcgg acgacgttat cggtactctg gcgcgcgaag ccgaaaaagc cgggcgtccg
     2581 gtgctgatca gcactggcga taaagatatg gcgcagctgg tgacgccaaa tattacgctt
     2641 atcaacacca tgacgaatac catcctcgga ccggaagagg tggtgaataa gtacggcgtg
     2701 ccgccagaac tgatcatcga tttcctggcg ctgatgggtg actcctccga taacattcct
     2761 ggcgtaccgg gcgtcggtga aaaaaccgcg caggcattgc tgcaaggtct tggcgggctg
     2821 gatacgctgt atgccgagcc agaaaaaatt gctgggttga gcttccgtgg cgcgaaaaca
     2881 atggcagcga agctcgagca aaacaaagaa gttgcttatc tctcatacca gctggcgacg
     2941 attaaaaccg acgttgaact ggagctgacc tgtgaacaac tggaagtgca gcaaccggca
     3001 gcggaagagt tgttggggct gttcaaaaag tatgagttca aacgctggac tgctgatgtc
     3061 gaagcgggca aatggttaca ggccaaaggg gcaaaaccag ccgcgaagcc gcaggaaacc
     3121 agtgttgcag acgaagcgcc agaagtgacg gcaacggtga tttcttatga caactacgtc
     3181 accatccttg atgaagaaac actgaaagcg tggattgcga agctggaaaa agcgccggta
     3241 tttgcatttg ataccgaaac cgacagcctt gataacatct ctgctaacct ggtcgggctt
     3301 tcttttgcta tcgagccagg cgtagcggca tatattccgg ttgctcatga ttatcttgat
     3361 gcgcccgatc aaatctctcg cgagcgtgca ctcgagttgc taaaaccgct gctggaagat
     3421 gaaaaggcgc tgaaggtcgg gcaaaacctg aaatacgatc gcggtattct ggcgaattac
     3481 ggcattgagc tgcgtgggat tgcgtttgat accatgctgg agtcctacat tctcaatagc
     3541 gttgccgggc gtcacgatat ggacagcctc gcggaacgtt ggttgaagca caaaactatc
     3601 acttttgaag agattgcggg taaaggcaaa aatcaactga cctttaacca gattgccctc
     3661 gaagaggccg gacgttacgc cgccgaagat gcggatgtca ccttgcagtt gcatctgaaa
     3721 atgtggccag atctgcaaaa acacaaaggg ccgttgaacg tcttcgagaa tatcgaaatg
     3781 ccgctggtgc cggtgctttc acgcattgaa cgtaacggtg tgaagatcga tccgaaagtg
     3841 ctgcacaatc attctgaaga gctcaccctt cgtctggctg agctggaaaa gaaagcgcat
     3901 gaaattgcag gtgaggaatt taacctttct tccaccaagc agttacaaac cattctgttt
     3961 gaaaaacagg gcattaaacc gctgaagaaa acgccgggtg gcgcgccgtc aacgtcggaa
     4021 gaggtactgg aagaactggc gctggactat ccgttgccaa aagtgattct ggagtatcgt
     4081 ggtctggcga agctgaaatc gacctacacc gacaagctgc cgctgatgat caacccgaaa
     4141 accgggcgtg tgcatacctc ttatcaccag gcagtaactg caacgggacg tttatcgtca
     4201 accgatccta acctgcaaaa cattccggtg cgtaacgaag aaggtcgtcg tatccgccag
     4261 gcgtttattg cgccagagga ttatgtgatt gtctcagcgg actactcgca gattgaactg
     4321 cgcattatgg cgcatctttc gcgtgacaaa ggcttgctga ccgcattcgc ggaaggaaaa
     4381 gatatccacc gggcaacggc ggcagaagtg tttggtttgc cactggaaac cgtcaccagc
     4441 gagcaacgcc gtagcgcgaa agcgatcaac tttggtctga tttatggcat gagtgctttc
     4501 ggtctggcgc ggcaattgaa cattccacgt aaagaagcgc agaagtacat ggacctttac
     4561 ttcgaacgct accctggcgt gctggagtat atggaacgca cccgtgctca ggcgaaagag
     4621 cagggctacg ttgaaacgct ggacggacgc cgtctgtatc tgccggatat caaatccagc
     4681 aatggtgctc gtcgtgcagc ggctgaacgt gcagccatta acgcgccaat gcagggaacc
     4741 gccgccgaca ttatcaaacg ggcgatgatt gccgttgatg cgtggttaca ggctgagcaa
     4801 ccgcgtgtac gtatgatcat gcaggtacac gatgaactgg tatttgaagt tcataaagat
     4861 gatgttgatg ccgtcgcgaa gcagattcat caactgatgg aaaactgtac ccgtctggat
     4921 gtgccgttgc tggtggaagt ggggagtggc gaaaactggg atcaggcgca ctaagattcg
     4981 cctgaacatg cccttttttc gtaagtaagc aacataagct gtcacgtttt gtgatggcta
     5041 ttagaaattc ctatgcaaca actgaaaaaa aattacaaaa agtgctttct gaactgaaca
     5101 aaaaagagta aagttagtcg cgtagggtac agaggtaaga tgttctatct ttcagacctt
     5161 ttacttcacg taatcggatt tggctgaata ttttagccgc cccagtcagt aatgactggg
     5221 gcgtttttta ttgggcgaaa gaaaagatcc gtaatgcctg atgcgctatg tttatcaggc
     5281 caacggtaga attgtaatct attgaattta cgggccggat acgccacatc cggcacaagc
     5341 attaaggcaa gaaaattatt cgccgtcctg cgtttcttct acaggctgca tctcgctaaa
     5401 ccaggtatcc agtttctgcc gcagcttgtc cacgccttgt ttcttcaacg aagaaaacgt
     5461 ttcaacctgc acatcaccgt taaacgccag tacagcttca cgcaccatat tcaattgcgc
     5521 tttacgtgcg ccgcttgcca gtttgtccgc tttggtcagc agcaccagaa cggcgatatt
     5581 gctgtctacc gcccactcaa tcatctgctg atccaaatct ttcagcggat ggcgaatatc
     5641 cattagcacc accagacctt gcaggctctg acgtttttcg aggtattcgc cgagcgcacg
     5701 ctgccatttg cgcttcatct cttccgggac ttccgcataa ccgtacccag gcaagtcaac
     5761 cagacgcttg ccgtcagcca cttcaaacag gttgataagc tgggtgcgcc ctggggtttt
     5821 tgaggtacga gccaggcttt tctggttagt cagcgtgttc agcgcgctgg atttacctgc
     5881 gttggaacgg cctgcaaaag ccacttcaat tccggtatcg gaaggtaggt ggcgaatatc
     5941 aggcgcactc atcacaaaat gcgtctgttg ataattcaaa ttagtcaaag cggtcgtctc
     6001 cgtcagtcaa agctttgtgg cgattatacc tgaaccataa taaaaggcgg atttttcggc
     6061 gtgagcgttg taagtaaaag ccatacgctt tgtgagacat tgccgatagt ctttatgcga
     6121 aatagcagag aaaattctgc gatgcatgtc aaataagcta tataaatcag tgaattgctt
     6181 ttatataaaa ctctgaaaat gtgcaaatac tgatggcggt tgattgtttg tttaaagcaa
     6241 aggcgtaaag tagcacccat agagcgagga cgctaacagg aacaatgact caggatgagg
     6301 gtcaggagcg ccaggaggcg aagacagagg attgtcagga agacaaacgt ccggagacgt
     6361 aattaaacgg aaatggaatc aacacggatt gttccggcta aaggaaaaac agggtgtgtt
     6421 ggcggcctgc aaggattgta agacccgtta agggttatga gtcaggaaaa aaggcgacag
     6481 agtaatctgt cgcctttttt ctttgcttgc tttctgttag attccgccgc aaatctatac
     6541 tgaataaaac ggctaaaaga cgaaccatta tgaaaccatc atcttcaaac tcacgcagca
     6601 aaggtcatgc aaaagcgcgt cgaaaaacac gcgaggagct ggatcaggaa gctcgtgatc
     6661 gcaagcgtca gaaaaaacgt cgtggtcatg cgccgggcag ccgcgcagcg ggcggaaaca
     6721 ccacgtcagg cagcaaaggc cagaacgcac caaaagatcc acgtattggc agtaaaaccc
     6781 ctattccatt gggcgtggct gaaaaagtca ccaaacagca caaaccgaag agtgagaaac
     6841 ctatgctttc accgcaggcg gagttggaat tactggaaac ggatgagcgt ctggatgcgc
     6901 tgctggaacg tctggaagca ggcgaaaccc tgagcgccga agagcaatcc tgggtggatg
     6961 ctaaactgga tcgtattgat gagttgatgc agaaactcgg cctctcttat gacgatgacg
     7021 aagaagagga agaagacgag aagcaagaag acatgatgcg tctgctgcgg ggtaactaac
     7081 ggattgccac cgtgggcctt cccgttctgc tgataaccct tccggttata tgttatctgg
     7141 tgtggttatt cgttaaacta cagcggttgt cgcggcgaca aaagtggctg cgcaaccggc
     7201 tgatgacccg aaacgggcat ccgccggtac gccgtagccg ccagagacgc catcggaagg
     7261 agtgagcatg tctgtacagc aaatcgactg ggatctggcc ctgatccaga aatataacta
     7321 ttccgggcca cgatacacct cgtacccgac cgcgctggag ttttcagaag acttcggcga
     7381 acaggcgttt ttacaagccg tggcgcgcta tcctgagcgt ccattatctc tctacgtaca
     7441 tatcccgttc tgccataagc tttgttactt ctgcggttgc aataagattg ttactcgcca
     7501 gcagcacaag gccgatcagt atctggacgc gctggagcaa gaaatcgtcc atcgtgcacc
     7561 gctgttcgcc gggcgtcacg tcagccaatt gcactggggc ggcggaacgc cgacgtatct
     7621 gaataaagcg caaatcagcc gcctgatgaa gctgctgcgc gaaaacttcc agttcaatgc
     7681 cgatgcggag atttcgatcg aagtcgatcc gcgggaaatc gaactggatg tactcgatca
     7741 tttacgcgcc gaaggcttta atcgcctgag catgggcgtg caggacttca acaaagaagt
     7801 gcaacgtctg gttaaccgcg agcaggatga agagttcatc tttgcactgc ttaaccatgc
     7861 gcgtgagatt ggttttacct ccaccaacat cgacctgatt tacggcctgc cgaaacagac
     7921 gccggagagt ttcgccttta ccctgaaacg tgtggcggag ctgaaccccg atcgtctgag
     7981 tgtctttaac tacgcgcatc tgccgaccat ttttgctgct cagcgcaaaa tcaaagatgc
     8041 tgacctgccg agtccgcagc aaaaactcga tatcctgcag gaaaccatcg ccttcctgac
     8101 gcaatcgggc tatcagttta tcggtatgga tcactttgcc cgtccggatg acgagctggc
     8161 ggtggcccag cgtgaaggcg tgctgcatcg taacttccag ggctacacca ctcagggcga
     8221 taccgatctg ctggggatgg gcgtttccgc catcagcatg attggcgact gctacgcgca
     8281 gaaccagaaa gagttgaagc agtactatca gcaagtggat gaacaaggca atgctctgtg
     8341 gcgtggtatt gcgctaacgc gtgatgactg tattcgccgc gatgtgatta agtcgctcat
     8401 ctgcaacttc cgtctggatt acgcccctat tgagaaacag tgggatttgc acttcgctga
     8461 ttactttgcg gaagatctca agctgctcgc cccgttagca aaagatgggc tggtggatgt
     8521 ggatgagaag ggaatacagg tgacggcgaa aggtcgcttg ctgatccgca acatttgcat
     8581 gtgctttgat acctatctgc gccagaaagc gcggatgcag cagttctcac gggtgattta
     8641 aatagtgaat ggcgcttcgt ttacaaagta gaaaacgaaa gcgccatcaa tgtgctgaga
     8701 acaagattgc ctgatgcgct acgcttatca ggcctacaaa acctgttgaa aatattgctt
     8761 ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaagagca cttcatcagc
     8821 aatctgaaac tcaactaaac agcccaatca ttgcggcaca cagcacggca gcaacggcag
     8881 tttgcggtgt gtggctgtca actgcgccac tcgataccag attaattatt gattccagca
     8941 taatgactct ccccgttttc cgggcaagat catactgaac ttatcggaac agtaaagcgt
     9001 aaaataccag caatttgcgc tcaataatca atctttacac acaagctgtg aatcactcca
     9061 tccccagctc ttttaactta cgcgtcaggg tgttgcggcc ccagccgagt agccgcgccg
     9121 cttcctgttt atgcccctgc gtatgtcgta acgcggtcgt cagtaacgtc cgctccagct
     9181 ctggctgcgc ttcggaaagc agattttgat gaccggaacg cagcgctctg tctgcccact
     9241 gcgcaagaag cgttgcccag ctgtccggtt gcatttgcga agtactctcc gcaaccgttg
     9301 attcaaacag ttcgccgggc aaatcctgaa tcaacacttc ctgcccggcg gccatcaccg
     9361 ttagccagcg gcaggtgttt tccagctggc gcacgttgcc tggccacgcc agacgcgtca
     9421 gagcagcttc ggtttccgga tgcagtaact tcgcttctac gcccagttcg cgcgcggcaa
     9481 cctgtaaaaa atggcgcgcc agacggggaa tatcttcccg acgttcgcgc agcggcggca
     9541 gatgaacgcg gataacgttc aggcggtgga acagatcctc acggaactta ccttcctgca
     9601 ctcgctgttc gagattctgg tgagtggcag cgataatacg cacatccact ttcaccggcg
     9661 catagccgcc aacgcggtaa aactgaccgt ctgccagcac gcgcagcaaa cgcgtctgca
     9721 catccagcgg catatcacca atttcgtcga ggaataatgt accgccatcg gcctgttcaa
     9781 aacgcccctg acgaatggta ttcgcgccag taaacgcgcc tttctcgtgg ccaaacagtt
     9841 ctgattcgat caaatctttt gggatagctg ccatattcag cgcgataaac ggcgctttgg
     9901 cgcgcggact gtggcgatgc agggcatgag cgaccagttc tttaccggtg ccggattcgc
     9961 cgttaatcag cacgctaata gaagaacgcg aaagccgacc gataatacgg aacacgtcct
    10021 gcatggctgg cgcttcgccg atgatatcgg tcgttgggcc gttaagctga atattacgcg
    10081 gctgctgctg ttcctggtaa tgactgatag cgcgctcaac cagcgccact gcttcgtcga
    10141 tatcaaacgg tttgggcaga taatcaaacg ccccttgttg ataggcgctg acggcagcat
    10201 ccagatcgga atgtgcggtc ataatgatga ccggaagcat tggatggcgc tgtttaatct
    10261 gcttgagcag cgccagcccg tccattcccg gcatacggat atctgaaagc agcacatccg
    10321 gcgttttgct cgccagcgcc tccagtactt ccgcgccgtt ctcaaatgtc gtacaggtta
    10381 aacccgctcc agcgagcgca cgttcaagca cccaacggat ggaactatcg tcatcgacta
    10441 cccagactat ccctcgttgc ataaacgtca cctttatttc ctgataggca ggtaaaccga
    10501 gaactcggta tgacctggcc aactggtaaa ttcaattttg cctgaatgct gatcaatcaa
    10561 attacgggcg atggataagc caagcccggt gccaccttcg cggccgctga ccatcgggta
    10621 aaacagcgta tcctgcaaat gaggcggaat gcccggcccg ttatcttcca catcaatccg
    10681 cgccgccagc cggtagcgct cgccgtgtaa ggtcagttga aacgcggtgc gggtacgcag
    10741 aatgatttca ccgccttccg gccccagcgc ctgtagcgca ttgcgcacaa tattcagcaa
    10801 gacctgttca atttgatccg ggtcgtgcgc cagttccggc aggctggggt cgtaatcacg
    10861 aatcaaccgc acgttgtccg gcagttccat cgacaccagc gtcaccacgc gttcagctac
    10921 tttgtgaata ctttcggtaa cgcgcgtacc gggcagctgc ggccccaaca gacggtcgac
    10981 cagatttcgc agccggtccg cctgttcgat aatcactttg gtatattcga gtagtgatgg
    11041 gtcaggtaac gctttgctga gcagctgcgc cgcgccacgt aaacctccaa gcggattttt
    11101 aatctcatgt gccaggccgc gcactaaatc acgggcagca acctgctggg cgtgctgtag
    11161 ctgttcctga cttaagcggc gctggttatc catcggagcc atctccagca ggatcatgcc
    11221 gtccggcata cgctgggccg tcacagaaag gatatgcgag cgcccgtcga tgaccagcgt
    11281 cacttcgtta tcggtaaaac cttgccccgc ctccagactt tcttgcatca gctcgatatt
    11341 taatgagaag tagctcaaca gttccggtaa cggtgtacca aacaatttgc gggagctttg
    11401 ggcgagcagt tgttgcgcgg cggggttggc gtaatggatc gccagattgt catcaattaa
    11461 caaaatactg ttaatcagcg agttgaggat ctgcccagca tcgggctgcg tgcctgttgc
    11521 cataaagcag tctcctgaac aggttgcacc attttagtgc attatagctt tttacggata
    11581 aaaagcgcga agcatcagag aattgacgga gaaaaaagcc catgcagaga tgggctgaaa
    11641 gtttccacgg caactaaatc ccggcgttgt tgcgccgggt agtacaactt caactattag
    11701 acgctgtagt acagctcaaa ctctaccgga tgcggagtca tacgcacgcg gtcatcttct
    11761 tcgcgacgta gagcgatgta cgcatcaatt gcttcgtcag tgaacacgcc accggctttc
    11821 aggaactcgc ggtccagatc cagttcgttc agtgcttctt ccagagagcc tgcaacctgt
    11881 gggatctctt tcgcttcttc tggcggcagg tcatacaggt ttttgtccat ggcttcgccc
    11941 ggatggatct tgttcttgat accatcaaga ccggccatca gcagggcagc aaagcacagg
    12001 tacgggttag ctgccggatc cgggaaacgt acttcgatac gacgtgcttt cggagaagaa
    12061 accaccggaa tacggataga cgcagaacgg ttacgcgcag agtaagccag cattaccggt
    12121 gcttcatagc ccgggaccag acgcttataa gagttggtgg tcgggtttgc cagggcgtta
    12181 atcgctttag cgtgtttgat tacgccgcca atgtagtaca gcgcctgctc agacagacct
    12241 gcgtatttgt cgcctgcgaa caggttaacg ccgtttttag acagagacat gtggcagtgc
    12301 ataccggagc cgttatcacc gaacatcggt tttggcataa aggtcgcggt tttaccgaag
    12361 cggtgcgcta cgttgtgcac aacatatttg tagatctgaa tttcgtcagc ttttttggtc
    12421 atagtattga agcgggtagc cacttcgttc tgaccagcag tcgctacttc gtggtgatgg
    12481 gcttcaacca ccagacccat ctgttccatc accagacaca tttcagaacg aatatcctga
    12541 gccgagtcta ccggtggaac cgggaagtaa ccgcctttca ctgccggacg gtgacctttg
    12601 ttaccacctt cgtattgggt ggaggagttc catgcgcctt cgatatcgtc gatagcaacg
    12661 tgggaaccgg agatagatga tccgaaacgg atgtcatcga acaggaagaa ttcaggttct
    12721 ggcccgaaca gtacggtgtc ggcaatgcca gtggaacgca ggtaatcttc ggcgcgcttc
    12781 gcaatggagc gcgggtcacg gtcatagcct tgcagggtgc caggttcaag gatgtcgcaa
    12841 cggataatca gggtggagtc ggcgaagaac gggtcaatca ctgcggtgga tgcgtctggc
    12901 atcagcacca tgtcggactc gttaatgcct ttccagccgc caatcgagga gccgtcaaac
    12961 attttgcctt cttcgaagaa ttcagcattc acctgatgag cagggatagt gacgtgctgt
    13021 tctttacctt tagtatcggt gaagcgcaaa tcaacaaact tcacttcgtg ctcgttcagc
    13081 atcgtcagta cgtgttcagc ggacatactt taactctcct ggattggtca tggtcgtcgt
    13141 ggtaacgaaa tctgcaatta ttttggccgt gtcgccgtaa aaaagataaa gcgaaatctg
    13201 tgccaacttt taaattgccc ctaaaaggcg ttatcatgcg caccatcgtg caaaagggct
    13261 gcaccacgat gtgaatgttg caccaatata gtgcttcaat ggaaacatta agcaccatgt
    13321 tggtgcaatg acctttggat aacccttttt atgctccgtg aaagcgatca caaagggact
    13381 ctgcaatact tgtttgcgga ggatgtttgt gatcctgttt tgtagtgcga ttaatccgtg
    13441 tacaataacg cgctatttct aatgcctgag gcaaagttgt gatcgaaaaa ttgcgtaata
    13501 tcgccatcat cgcgcacgta gaccatggta aaaccaccct ggtagacaag ctgctccaac
    13561 aatccggtac gttcgactct cgtgccgaaa cccaagagcg cgtgatggac tccaacgatt
    13621 tggagaaaga gcgtgggatt accatcctcg cgaaaaacac cgctatcaaa tggaatgatt
    13681 accgtatcaa catcgttgat accccggggc acgccgactt cggtggtgaa gttgaacgtg
    13741 taatgtccat ggtagactca gtgctgctgg tggttgacgc atttgacggc ccgatgccgc
    13801 aaacgcgctt cgtaaccaaa aaagcgtttg cttacggcct gaagccgatt gttgttatca
    13861 acaaagttga ccgccctggc gcgcgtcctg attgggttgt ggatcaggta ttcgatctgt
    13921 tcgttaacct cgacgcgacc gacgagcagc tggacttccc gatcgtttac gcttctgcgc
    13981 tgaacggtat cgcgggtctg gaccacgaag atatggcgga agacatgacc ccgctgtacc
    14041 aggcgattgt tgaccacgtt cctgcgccgg acgttgacct tgacggtccg ttccagatgc
    14101 agatttctca gctcgattac aacagctatg ttggcgttat cggcattggc cgcatcaagc
    14161 gcggtaaagt gaagccgaac cagcaggtca ctatcatcga tagcgaaggc aaaacccgca
    14221 acgcgaaagt cggtaaagtg ctgggccacc tcggtctgga acgtatcgaa accgatctgg
    14281 cggaagctgg cgatatcgtg gcgatcacgg gccttggcga actgaacatt tctgacaccg
    14341 tttgcgacac gcaaaacgtt gaagcgctgc cggcactctc cgttgatgag ccgaccgttt
    14401 ctatgttctt ctgcgttaac acctcgccgt tctgcggtaa agaaggtaag ttcgtaacgt
    14461 ctcgtcagat cctggatcgt ctgaacaaag aactggtaca caacgttgcg ctgcgcgtag
    14521 aagaaaccga agacgccgat gcgttccgcg tttctggtcg tggcgaactg cacctgtctg
    14581 ttctgatcga aaacatgcgt cgtgaaggtt tcgaactggc ggtatcccgt ccaaaagtaa
    14641 tcttccgtga aatcgacggt cgtaaacaag agccgtatga aaacgtgact ctggacgttg
    14701 aagaacagca tcagggttct gtaatgcagg cgctgggcga acgtaaaggc gacctgaaaa
    14761 acatgaatcc agacggtaaa ggccgcgtac gtctcgacta cgtgatccca agccgtggtc
    14821 tgattggctt ccgttctgag ttcatgacca tgacttccgg tactggtctg ctgtactcca
    14881 ccttcagcca ctacgacgac gtacgtccgg gtgaagtggg tcagcgtcag aacggcgtac
    14941 tgatctctaa cggtcagggt aaagcggtcg cgttcgcact gttcggtctg caggaccgcg
    15001 gtaagctgtt cctcggtcac ggtgcagaag tttacgaagg tcagattatc ggtattcaca
    15061 gccgctctaa cgacctgact gtaaactgcc tgaccggtaa gaaactgacc aacatgcgtg
    15121 cttccggtac tgacgaagct gttgttctgg ttccgcctat ccgcatgact ctggaacaag
    15181 ctctggagtt catcgatgat gacgaactgg tagaagtgac tccgacctct atccgtattc
    15241 gtaaacgtca cctgacggaa aacgatcgtc gtcgcgccaa ccgcgcaccg aaagacgatt
    15301 aatttcgttt tttagttata aaaaacctgc catcgct
//
LOCUS       DAZPJR010000097        15084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPJR010000097 DAZPJR010000000
VERSION     DAZPJR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.098"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHVB7_002758"
     CDS             <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHVB7_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1914698.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="ACHVB7_002759"
     CDS             complement(984..1337)
                     /locus_tag="ACHVB7_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1914699.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHVB7_002760"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHVB7_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1914700.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="ACHVB7_002761"
     CDS             complement(3165..3458)
                     /locus_tag="ACHVB7_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR1914701.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHVB7_002762"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHVB7_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR1914702.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /locus_tag="ACHVB7_002763"
     CDS             complement(5012..5482)
                     /locus_tag="ACHVB7_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR1914703.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="ACHVB7_002764"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="ACHVB7_002764"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR1914704.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHVB7_002765"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHVB7_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR1914705.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="ACHVB7_002766"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="ACHVB7_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR1914706.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHVB7_002767"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHVB7_002767"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR1914707.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="ACHVB7_002768"
     CDS             10838..11413
                     /locus_tag="ACHVB7_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1914708.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="ACHVB7_002769"
     tRNA            11520..11595
                     /locus_tag="ACHVB7_002769"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            11838..11952
                     /locus_tag="ACHVB7_002770"
                     /pseudo
     CDS             11838..11952
                     /locus_tag="ACHVB7_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            12212..12511
                     /locus_tag="ACHVB7_002771"
     CDS             12212..12511
                     /locus_tag="ACHVB7_002771"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR1914709.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHVB7_002772"
     CDS             complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHVB7_002772"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR1914710.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            12694..13749
                     /gene="cadC"
                     /locus_tag="ACHVB7_002773"
     CDS             12694..13749
                     /gene="cadC"
                     /locus_tag="ACHVB7_002773"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR1914711.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="ACHVB7_002774"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="ACHVB7_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR1914712.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAZPJR010000098        14965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPJR010000098 DAZPJR010000000
VERSION     DAZPJR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.099"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHVB7_002775"
     CDS             <1..196
                     /locus_tag="ACHVB7_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914713.1"
                     /translation="KAILTDGLFALAQKKMPDATLRVLYSHICQIQQRIRFPQLAHFH
                     TCPPYQKFILKLLNNHFPAN"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="ACHVB7_002776"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="ACHVB7_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR1914714.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="ACHVB7_002777"
     CDS             complement(1608..1931)
                     /locus_tag="ACHVB7_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR1914715.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHVB7_002778"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHVB7_002778"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR1914716.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHVB7_002779"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHVB7_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR1914717.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHVB7_002780"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHVB7_002780"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR1914718.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHVB7_002781"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHVB7_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR1914719.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="ACHVB7_002782"
     CDS             7531..8568
                     /locus_tag="ACHVB7_002782"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1914720.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHVB7_002783"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHVB7_002783"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR1914721.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="ACHVB7_002784"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="ACHVB7_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR1914722.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHVB7_002785"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHVB7_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR1914723.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="ACHVB7_002786"
     CDS             10739..11620
                     /locus_tag="ACHVB7_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1914724.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHVB7_002787"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHVB7_002787"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR1914725.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="ACHVB7_002788"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="ACHVB7_002788"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR1914726.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="ACHVB7_002789"
     CDS             complement(14017..>14965)
                     /locus_tag="ACHVB7_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR1914727.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3785 c         3921 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgccc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAZPJR010000099        14726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPJR010000099 DAZPJR010000000
VERSION     DAZPJR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.100"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1191)
                     /locus_tag="ACHVB7_002790"
     CDS             complement(<1..1191)
                     /locus_tag="ACHVB7_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1914728.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDV"
     gene            1840..2355
                     /locus_tag="ACHVB7_002791"
     CDS             1840..2355
                     /locus_tag="ACHVB7_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1914729.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            2573..3301
                     /gene="artP"
                     /locus_tag="ACHVB7_002792"
     CDS             2573..3301
                     /gene="artP"
                     /locus_tag="ACHVB7_002792"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR1914730.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            3319..4050
                     /gene="artI"
                     /locus_tag="ACHVB7_002793"
     CDS             3319..4050
                     /gene="artI"
                     /locus_tag="ACHVB7_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR1914731.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            4057..4773
                     /gene="artQ"
                     /locus_tag="ACHVB7_002794"
     CDS             4057..4773
                     /gene="artQ"
                     /locus_tag="ACHVB7_002794"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR1914732.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            4773..5441
                     /gene="artM"
                     /locus_tag="ACHVB7_002795"
     CDS             4773..5441
                     /gene="artM"
                     /locus_tag="ACHVB7_002795"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR1914733.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            5733..6464
                     /gene="artJ"
                     /locus_tag="ACHVB7_002796"
     CDS             5733..6464
                     /gene="artJ"
                     /locus_tag="ACHVB7_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR1914734.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(6639..7766)
                     /gene="rlmC"
                     /locus_tag="ACHVB7_002797"
     CDS             complement(6639..7766)
                     /gene="rlmC"
                     /locus_tag="ACHVB7_002797"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR1914735.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(7807..8295)
                     /locus_tag="ACHVB7_002798"
     CDS             complement(7807..8295)
                     /locus_tag="ACHVB7_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR1914736.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(8355..9200)
                     /gene="potI"
                     /locus_tag="ACHVB7_002799"
     CDS             complement(8355..9200)
                     /gene="potI"
                     /locus_tag="ACHVB7_002799"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR1914737.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(9197..10150)
                     /gene="potH"
                     /locus_tag="ACHVB7_002800"
     CDS             complement(9197..10150)
                     /gene="potH"
                     /locus_tag="ACHVB7_002800"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR1914738.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(10160..11293)
                     /gene="potG"
                     /locus_tag="ACHVB7_002801"
     CDS             complement(10160..11293)
                     /gene="potG"
                     /locus_tag="ACHVB7_002801"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR1914739.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(11388..12500)
                     /gene="potF"
                     /locus_tag="ACHVB7_002802"
     CDS             complement(11388..12500)
                     /gene="potF"
                     /locus_tag="ACHVB7_002802"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR1914740.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(12851..13327)
                     /locus_tag="ACHVB7_002803"
     CDS             complement(12851..13327)
                     /locus_tag="ACHVB7_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR1914741.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(13415..14317)
                     /gene="rimK"
                     /locus_tag="ACHVB7_002804"
     CDS             complement(13415..14317)
                     /gene="rimK"
                     /locus_tag="ACHVB7_002804"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR1914742.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(14378..>14726)
                     /gene="nfsA"
                     /locus_tag="ACHVB7_002805"
     CDS             complement(14378..>14726)
                     /gene="nfsA"
                     /locus_tag="ACHVB7_002805"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR1914743.1"
                     /translation="GVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPR
                     LPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPF
                     ILDYLHKQGWATR"
BASE COUNT         3836 a         3811 c         3473 g         3606 t
ORIGIN      
        1 aacatcacct aaactgccgt tttcaaaaca acattttttc aatatgcttt cattcacaac
       61 agtgtgtgat aagtagagat tacttatgtt ttgctttatt aatgccacgt cattaaaatg
      121 actatattta atgaaaaaac catcattgag tagtgtttta agattgacta aattacttgc
      181 atcttcacgt accatcgtgg caactggaac aggcagagac atgaaaactt cttcattcac
      241 catcaagtga atcatgcatt gcgttccctt tgtcatatcc acacatacgc tacaaatatc
      301 gtggtaatgc gaagagcgca tttcaggaaa cagattaaac acggtatctt gaaaggcact
      361 aaatcgtgtt aataaatcct gataatgaac aggttttgtt tcaaaattat catcagcaaa
      421 tccaagcact attgtatgct ctacatctaa cgcagtttca aatggctctt cttctgtaaa
      481 atcattattt aaaacctcac ctgacgtatt atcactgtca taaaaagtgt tcatcaatgc
      541 gatattgttt gttacagcgt ctttttcgat attctcacta attgcatatc gcctgctata
      601 atcataaaaa tgaataaacg cgccctttat aaaggatata tattgttctt tgttaaagtc
      661 aatacggctg tataattttt caaaaatcac attcgcaatt ttactaccaa tggtatgcct
      721 gtataataat gggtgcgatt caatcgtaaa atgcttgtct aattgatctt gtaatttatt
      781 ctcatagtcc agagggaaaa gaaactgttg tctttcaagt ttcccattcg atacattacg
      841 tattccaata gagattcttt cattacccgc ctttatttca acttcgttat cttctgattt
      901 acccagatat tttttaacat cagtctcgaa agcattacta aaaaaagtat taaattggta
      961 attcgctgat ggcatatttt ccgcgagcaa cacatcattt tttatgagat cactgtcagc
     1021 atttattgct aatatcagat gcaagtagat ggtatcccat gtagacaact cacccttacg
     1081 actggaattt aatatcgcga aaaattccct cagtgaatcc aggaaattat cactttgatt
     1141 tagttgtgtg cctggaaatg atgatgcttg atcagatata ttgtttatca tattaaaaat
     1201 cacccaagaa aaacgataat ccaccgaact cctcgtgctt tataattata aaagcacaac
     1261 gccttttata ttcatattaa ttaacgagat actgaaacaa cttaatttat tcttcgctaa
     1321 aattcattag cttaaattct ttagccacaa ttgtagcata aataatatta tcgagagtca
     1381 aagctgggac tatcaaattt cgctcatttt caaaaataca acgtaatttt ggcaatcatc
     1441 ttaatcgcag gatctcttat cgcatgtgaa aaattttgta atgattagac aataaccgcg
     1501 ttcatttatc acgcgttata aatgtgaaac tggcttcttg cagatgctct taacctctac
     1561 aggaggattt ataccaccat ttcagaacta tcatatttac cttaaatgat catttcagcc
     1621 atataacacg ctcaacctca caaatggttt acactgtcta ttgtttgggt aatttaagta
     1681 agtcagctac gagcaacctt gagtggatag ccagcaatgg actcaaaccc aatcaggtgt
     1741 tcagattgtt tcagaattgt attaaaaaca atgtattaaa taatctgata gcgtttggtc
     1801 gtagtgtgac gcagacagca agctaataag gaaatgatta tgcgctaccg ttcactatct
     1861 ctacttattc catgtgcact gctgctgagt gcctgtacca ccgtcacgcc agcttataaa
     1921 gataacggtc cccgcaccgg ttcctgtgtc cagggggggc ctgacagcgt ggctcaacaa
     1981 ttctatgact accgcattca acatcgcagc aatgacatta ccgccctgcg cccctatttg
     2041 agtgacaaac tggcaaccct tcttagcgat gccagccgcg ataatagtca tcgtgaattg
     2101 ttaagcagcg atccgttctc cagtcgaacc accttgccag atagcgccca cgttgccagt
     2161 gcatcgacta tccctaatcg tgatgcccgt aacattccat tgcgcgtaga tctcaaacag
     2221 ggtgaccagg gttggcagga tgaagtgtta atgattcagg aaggccagtg ttgggtcatc
     2281 gacgatgtgc gctacttagg cggcagcgtt catgccacgg cgggtacgct ccgtcagtcc
     2341 atcgagaatc gctaacgcct tgatcaacca ataaaccccg taaattgtcc ggcatttcgt
     2401 gaggaatgcc gacatttatg ctcgccgacc accgcccccg ttattttgtg ctatgtttat
     2461 tgaataatgc gctttgcttt taacttttaa agcagaaata ttgcataatt attctgtcaa
     2521 aggtactatc ttcgacctca atagctatca gactgccagt atacgagtgt caatgagtat
     2581 tcaattaaac ggcattaatt gcttctacgg cgcgcatcag gcgctgttcg atatcacgct
     2641 ggattgccca cagggcgaaa cgctggtgtt acttggcccc agcggcgcgg gtaaaagctc
     2701 gctgctgcgt gtactcaatc tgcttgagat gccacgctcc ggtacgctca acattgcagg
     2761 caaccatttc gatttcacca aaacaccctc tgacaaagcg attcgcgatt tgcgtcgtaa
     2821 cgttggcatg gtgtttcagc aatacaacct gtggccgcat ctgaccgtgc agcaaaacct
     2881 gattgaagcg ccctgccgtg tactggggtt gagtaaagat caggcgctgg cccgtgcaga
     2941 aaaactgctg gaacgtctgc gtctcaaacc ttatagcgat cgttacccgc tgcatctttc
     3001 tggtggtcag cagcagcgtg ttgctattgc ccgtgcgttg atgatggaac cgcaggtact
     3061 gctgttcgat gaaccgaccg ccgcactgga cccggaaatt acggcacaaa tcgtcagcat
     3121 cattcgtgag ctggcagaaa cgaatattac ccaggtgatc gtcacccacg aagttgaagt
     3181 ggcgcgtaaa accgccagcc gagtggtgta tatggaaaat ggtcatatcg tagaacaagg
     3241 cgacgcgagc tgctttaccg agccgcaaac cgaagcattt aaaaactatc tctctcacta
     3301 agattcggga taacaacaat gaaaaaagtt ctgattgccg cgttaattgc aggttttagt
     3361 ctttccgcca cagctgccga aaccattcgt tttgctaccg aagcatccta tcctccgttt
     3421 gaatcgattg atgcaaacaa ccagatcgtt ggttttgacg tcgacctggc acaagcgctg
     3481 tgtaaagaga ttgatgcaac ctgcactttc tctaaccagg cgtttgacag cctgatccca
     3541 agcctgaaat tccgtcgcgt agaagccgtg atggcgggca tggatatcac tccggagcgt
     3601 gaaaagcagg tgctgtttac caccccgtac tatgacaact ctgccctgtt tgtgggtcag
     3661 caaggcaaat acaccagtgt tgatcagctg aaaggcaaaa aagtcggcgt acagaacggg
     3721 acgacacacc agaaattcat tatggataag cacccggaaa tcactaccgt tccgtatgac
     3781 agctaccaga acgcaaaact ggatctgcaa aacgggcgta tcgacggcgt cttcggtgac
     3841 accgcagtgg tcactgagtg gctgaaagat aacccgaaac tggcggcggt gggcgacaaa
     3901 gtgaccgata aagattactt cggcactggc ctcggcatcg cggtacgtca gggcaacact
     3961 gagctgcagc agaaactcaa cactgcgctg gaaaaagtga agaaagatgg cacttacgaa
     4021 accatctaca acaaatggtt ccagaagtaa ttcctgatga atgaattttt tcctttagca
     4081 agcgccgccg ggatgaccgt cggccttgcc gtttgtgcat tgattgtcgg gctggcgctg
     4141 gcgatgttct ttgcggtatg ggagtcggca aaatggcgtc ctgtcgcgtg ggcaggttca
     4201 gcgctggtaa ccattctgcg tggcctgcca gaaattctgg tggtgctgtt tatctatttt
     4261 ggctcctcgc agctgctgct gacgctttcg gatggcttca ctatcaatct tgggttcgtg
     4321 cagatcccag tgcagatgga cattgagaac ttcgacgtta gcccgttcct ttgtggtgtc
     4381 atcgctctgt cactgctgta tgccgcctat gcctcgcaaa cgcttcgggg cgcgttgaaa
     4441 gcggtgccgg tgggtcagtg ggagtctggt caggcgctgg ggctgtcgaa atcggctatc
     4501 tttttccgtc tggtgatgcc gcagatgtgg cgtcatgcgc tgcctggcct cggtaaccag
     4561 tggctggtgc tgctgaaaga taccgcgctg gtcagtttga ttagtgtgaa tgatttaatg
     4621 ctgcaaacaa aaagcatcgc tactcgtacc caggaaccat ttacctggta cattgtggcg
     4681 gcggcgattt acctggtgat caccctgctc agtcagtaca ttctcaaacg cattgacctg
     4741 cgcgcgacac gttttgagcg gaggcccagc taatgtttga gtatttaccc gaactgatga
     4801 aagggctaca caccagcctg acgctaaccg ttgcctcgct gattgtggca ctgattctgg
     4861 cattgatttt taccatcatc ctgacgctga aaacgccggt gctggtgtgg ctggtgcggg
     4921 gttatatcac gctgtttacc ggtacgccgc tgctggtgca gatcttcctg atttattacg
     4981 ggccgggcca gtttccgacg ttgcaggagt atccggcact gtggcatttg ttgtcagaac
     5041 cgtggttatg tgcgctgatt gcgttatcgc tgaacagtgc ggcgtatacc acgcagctgt
     5101 tttacggtgc aattcgtgcg atcccagaag gtcagtggca gtcctgtagc gccctgggaa
     5161 tgagcaaaaa agatacgctg gcgctcctgc tgccgtatgc ctttaaacgc tcgctctctt
     5221 cttattccaa cgaagtggtg ctggtgttca aaagtacctc tctggcttac accattacgc
     5281 tgatggaagt gatgggatac agccagttgt tgtacggacg cacctacgat gtaatggtgt
     5341 tcggtgcggc agggattatt tacctggtcg tcaacggcct gctgacgctg atgatgcgtc
     5401 tgatcgagcg caaagcactg gcattcgaac ggcggaatta atgtagctgc gtacattcgc
     5461 cgggggggtt tgtaggtctg ataagacgcg ccagcgtcgc atcagacatc agcacggtgc
     5521 ctgtgccgga tgcggcgtgg acgccttatc cggcctacat agtgcataaa ttcttataat
     5581 gaagacggac aactcactaa gttatccgtc tttttttatt tcatttaaat tatttaatca
     5641 tatttattgc atataaattc acttgatggc attgttatcc catgccgcag acacggccaa
     5701 aaatcataag attgacagac gggagttcca tcatgaaaaa gttagttctt gccgctttac
     5761 ttgcttcctt tactttcggt gcttctgccg cagagaaaat caattttggc gtttcagcca
     5821 cctatccacc ctttgaatct ataggtgcta ataatgagat tgtcggcttt gatatcgatc
     5881 tggcaaaagc cttgtgcaaa caaatgcagg cagaatgtac ttttactaat cacgcgttcg
     5941 acagcctgat cccgtcactg aaattcagaa aatatgactc cgtaatctcc ggtatggata
     6001 tcacgcccga gcgtagcaag caggtatcgt ttaccacgcc ctactatgaa aactcagccg
     6061 tcgtgattgc caaaaaagat acctacaaaa cgtttgccga tctgaaaggc aaacgtattg
     6121 ggatggaaaa cggcaccacg caccagaaat atattcagga tcagcacccc gaagtgaaaa
     6181 ctgtctccta tgacagttat cagaatgcct ttatcgatct gaaaaatggt cgtattgatg
     6241 gggtatttgg tgacacagcg gtggtaaacg aatggctgaa aaccaatccg caactgggcg
     6301 ttgctactga aaaagtgacc gatccgcaat acttcggcac cggtctgggc atcgctgtac
     6361 gtccggataa caaagctctg ctggaaaaac tgaataacgc gctggcagca attaaagctg
     6421 acggtacata tcagaaaatc aatgaccagt ggttcccgca gtaattgaga ctgatgacaa
     6481 aagtaagatt gcctgatgcg ctgcgcttat ctggcccaca tcaactctgc aatgtattga
     6541 tttagcatga ttttgtaggc cggataaggc gtacacgccg catccggcat gaacaaagcg
     6601 cacctgatca gcaatctgaa gccgcacctg cggctttttt attgcttcac cagcagcgtc
     6661 atcacttcat agtgcgcggt gtgcgggaac atatcaaaaa gctgtacccg ttcgatgcgg
     6721 taacctggca gttcgcggat atctttcgcc atggtttggg cgttacagct ggagtagata
     6781 ataaaacgcg gtgccatcgt tgagagataa tcacacagcg gtttaccaat gccgcggcgc
     6841 ggcgggttaa ccagcaccag ctccggcaca tccccctgag cggtggcaaa ctgagtggag
     6901 tccagcgcct gaaattgcaa acgcgttaag cccagttcag cggctgactg ctttgcacag
     6961 gcaatggcct ctgatgcaat ttcgatcccg gttaactgca tgtcaggcgt cgcgcagtgt
     7021 aaaccaaagc cacccacacc gcagaacaga tcccacatat gtttaactgg cagctgccgt
     7081 acccagtcgc gcgcggtagc gtacagctgg ctggcgaccg ccggattagt ctggaagaaa
     7141 ctttgcggac ggatccacag cggtacgtca ttaaaacgct ctgccagcgc ctgttgttca
     7201 gtcaggtaga tctccgtctc cccttccata atcgccatat gtaccggctg aatattgacg
     7261 gtaataactt tcagttgcgg tagttgttcc tgtaaccacg gcagcgcctt acgcagttgt
     7321 gccagtttgg tttcagaacg cagtacaaag cgcagcatca tgccgccatc gctctggctt
     7381 tcagtcagca gaatgtattt cagttcgcca cgcttacgcg ccacgttgta gggcgttaac
     7441 cccgcacggg cgataaacgg ttttagcgcc gcaaaaacgg gcgcaaaaga ggcaggataa
     7501 agcgggcagt cacaaaggtc ttccggcgtg ccatcgcgat gcagcatacc gagcagtggt
     7561 ttttcaacgc taccactcac caccattttg gctttattac gaaacccttg ttccgggcct
     7621 gacaccggcg cgcaccattc ctcaaccgga aaatcggcga gcagattttt aagatcggcg
     7681 gttttagcgg agagttgctc tggaatcggc tgcgttatcc actgacagga acgacagcga
     7741 cccgcgtcgt aaagtgcgca ctgcatacat tgaccttcac atcatcaggg gcgacgatta
     7801 tacacattat tgcaactgga agaaccgccg actggtcgag ggaacgaaca gcagcatcag
     7861 gatgagcata tccggcagct tctgcagcat caggctatgg aagatttcac gttttgattc
     7921 accgggaatg ctgaacagct ccggataacc gtaccccagc gaagccgccc acaagtaact
     7981 tgcggcggtg atttgtgtca gcagatatag ccagcgcgcc cagcgacgcc ctttcaccag
     8041 tgaaaacgca caccagatct caatgaaaac cagcaccaga ctgcttaaaa agaccagcgt
     8101 taaactccag gtttgtacgc tgcgatgaat gaactcgcca ataccgcgca cgcccagcgt
     8161 attgaaaatc atcagcacgt cgaggccacg gatcataata atggcgagcg ccgccacctg
     8221 caccagcgca ggcacattca ggcgagcatg agatgaagat gttttcttaa aaaatcccaa
     8281 cgtttcgtct tccatgaaaa caatgccgcg gcatgcgcgg cattatgtag ccaggttggc
     8341 aaattttagt gtcttcagcc atgtcttgca cgctggatat cgcgtatccg ctgtttttct
     8401 gcgcgagcca tcagatacca ggcgataaat ccgacaattc cgaccgcacc gagaattaac
     8461 gttgcaaggg cgttgatttc cggattcact cccatccgca cgctggaaaa gaccagcatc
     8521 ggtaacgtgg tggctcccgg cccggaaaca aagctggcga tcaccagatc atcaagcgac
     8581 aaagtaaaag ccagtaacca gccagaaatg atcgcgggca tgatcatcgg tagcgtaatg
     8641 acgaaaaaca ctttcagcgg cgtcgcaccg agatccatcg ctgcttcttc tatcgagcga
     8701 tccagttccc gcaatcttga agaaataacg accgccacat aagccgtaca aaaagtgaca
     8761 tgcgccagcc agatggtgag cataccgcgg tccgcaggcc agccaatggc atgagcaagc
     8821 gcgacgaata ataacaacag cgacaagccc gtgatgccat ctggcatcac cagcggcgcg
     8881 gtgatcataa aggcaaaacc atttgatccg cgaaacctgc caaaacgcac cagcaccacc
     8941 gccgcaatag tcccgaggat cgccgccgcc gttgccgcac aggcagcaat tgtcaggctt
     9001 aaaccaaccg cactcatcat cgcgtcatcg cgcaataact caccatacca gcgcgttgac
     9061 cagccggccc acaccgtcac cagcttcgag ctgttaaacg aatagataac cagcatcagc
     9121 attggcgcgt agagaaaggt aaagcccagc agcaaaatca caatccgcca gggcgaacga
     9181 actaccggta aattattcat ccgtgttctc ccacgctttt ttgctggtgt ttgtggaacc
     9241 acattatcgg cacaattagc agcagcaaca tgatgatcgc taccgccgag gccaccggcc
     9301 agtcgcggtt attgaaaaac tcttgccata acacgcgccc gatcatgatg ctgtccgggc
     9361 caccgagcag ttccgggatc acaaactcgc ccacagccgg gataaacacc agcatcgatc
     9421 cggcaataat cccaccttta gtcagcggca cgatcacagt aaagaacgtt ttcagcggtc
     9481 gtgcaccgag atccagcgct gcttccacca gcgaataatc aatacgaatc aacgcggtat
     9541 aaatcggcag taccataaac ggcacgtaag cgtaaacaat gccgatataa acggccagat
     9601 tggtatgcag aatggtcagc ggttgatcga taacccccag ccacagcaga aaattattca
     9661 gcacaccgtt gttttttaat attcccatcc aggcataaac gcggatcaga aacgaggtcc
     9721 acgacggcag gatcaccagt agtaataaaa tattacgggt cgaaggctta ctgtgcgcca
     9781 ccgcccacgc cagcggatag ccgatcagta aacagcaaaa tgtcgaaatc gccgccacct
     9841 gtaacgactg gagataagca tcaaaataga gcggatcgtc ggtcagttgc agaaaattac
     9901 cgagattaag agtgatggaa agttgcccgt cagcccactc catcagttcg gtataaggtg
     9961 gaatagcgcg cgccatctct cccaggctta ttttaaagac aatcagaaat ggcagcagaa
    10021 acagcaagat caaccagata tatagcaacg caatgaccag tttgcgccca tgcttcattt
    10081 gcagctgcga caaccacagc ttaaatccgc ccggcggttt cgactgggca gcaggttcaa
    10141 gtgtactcat ctttgctcct taaaccgtca gcaccacaca gctgtccact tcccagcaca
    10201 aacgcacttc gtcgccccag gtcggtaacc ctttacgatg acgatgggcg ttttgtagct
    10261 gggcgctgat catctgccca cttttcagtc gaacgtgata caccgaaaga tcgccgagat
    10321 aggcaatgtg tatcacctcc cccaccgcga agttacaacc attggcgggc ggctcttcgc
    10381 aaagcatgat tttttccggg cgcagcgcta cgtgtaccgg cacgttatcg accactgaag
    10441 catccgcatc gactttcagt ggatgcacca gccccggcga atcaagcacc aggccatctt
    10501 cctgacgctc tttgagtaca ccttcaaaga cattaaccga accgataaat tcagcgctgt
    10561 agcgggtagt tggatgctcg tagatctctt ccggctcgcc aatctggaca aatttcccgc
    10621 gattcataat ggcgatacgc cccgccatgg tcatcgcctc ttcctgatcg tgggtgacca
    10681 tcacacaagt cacaccgacg cgctccagaa tatccaccac ttcaagctgc atccggtcac
    10741 gcagcttttt atccagcgcg cccatcggct catcgagcag taatagtttc gggcgtttgg
    10801 caaggcttcg ggccagggcc acacgctgtc gctgaccacc ggaaagctga tgcggtttgc
    10861 gtttggcgaa ctcctgcatg tgcaccaggc cgagcatctc atggacccga ctggcaattt
    10921 ccgctttcgg tagtttgtcc tgtttcaggc caaaagcgat gttctgttcc acggtcatat
    10981 gcggaaacag cgcgtaagac tgaaacatca tattgatggg gcgcaggtaa ggcggcacct
    11041 gtgacaaatc gacgccatcg agcattatct gcccggcaga aggttgttca aaacctgcca
    11101 gcatacgcag cagcgtggac ttgccacagc cggatgcgcc cagcagcgcg aagatttcac
    11161 ctttgtagat ggtcaggctg acatcatcga ccgcatgctg accatcgtag gatttggtga
    11221 ggttgcggat ttctaatagc ggcgtcagcg ccttacgggt tttcgcctgc gggcgaggga
    11281 tagcgtcatt cacggctgct ctccggcatt gaatgtgtgc aagcgcacca tgatggtgcg
    11341 aattgccggc tggtgtggtg cgcccgcccg gcatctaagg ctgcggatta ttttccgctc
    11401 ttcactttgg tccacgcgcg ggtgcgcaca cggtcgattt tcggatcctg tactttcaga
    11461 gtgaatagct tcgcacgaac gtccgccgga ggataaatac ccgggttctc acggacttcc
    11521 gcactcacca gcggcgtggc tgctttattg gcgttggcat agaacacatg gtcggaaata
    11581 tgcgctacta catccgggcg cagcaggtaa ttcaggaact gataggcttc gtctttgttt
    11641 ttggcatccg caggcatggc gaatacatca aagaacgcca tcgccccttc ttttggaatc
    11701 gagaacgaga cattcactcc attcttcgct tccttcgcgc ggtttgacgc ctgccagaca
    11761 tcacctgccc agccgatagc gacgcaaata tcgccgtttg ccaggtcgtt aatgtattga
    11821 gatgaatgga aataacgaat gttcgggcgc agctttaaca gcagatcggt tgccggtccg
    11881 gtgtaatcat ccgctttagt gctgttggga tctttgccga gataattcaa cacggtagca
    11941 aaaacttctt ctggcgcatc caggaaagag acaccgcagc ttttcagttt ttccagattt
    12001 tcaggtttga ggatcaagtc ccagctatcg acgggcgcgt tttcgcccag cacagcttta
    12061 actttatcaa cgttatagcc aatcccggtc gtcgcccaca tatagggcat agcaaattta
    12121 ttgtcgggat cgtgtttggc gaccagcttc agcagctccg ggtcgagatt cttccactcc
    12181 ggcaatttgc ttttgtccag cggctggaaa actcccgcag tcaactggcg ttcaagaaag
    12241 ctggcagatg gaaccaccaa gtcaaagccg gtgctacccg ccattaattt gccttccagt
    12301 acttcgttag agtcgaaaac atcgtagacg actttaatac cggtttcttt ttcaaaattg
    12361 gccaccgtgt ccggggcgat ataatcagac cagttataaa tgtggagtgt tttttgttca
    12421 gccgcgagcg tgccgacaga gacggccatc agagcacccg cagccagacc cgaaagccat
    12481 tttttattta aggcggtcat atctctttcc ttctgaaagt tcgttaacaa acgaacaaaa
    12541 gttgtcacac taaaagatat gcaataaacg tgcatatttt ttatcggcgg ggtgtaagaa
    12601 aagagaactc tctcccggcc agcactgctc gctatataaa agcatcgcaa gaataacttt
    12661 agccagggtt tgagactata gctcggatta aaaaacggta tttaacaaat ttttatgcgt
    12721 atttgctcta tgtgataaag ccaaatcgac acaagagaag tgaaataatc tttaagaaaa
    12781 ggttaaaaac agcagataat tttgcagcgc gcagccactg caccagcgac tgcgtgctcg
    12841 aaaggagtta tcagtggagg aaatagtttt cggtaacgtt gttttgccct tcatcgtcag
    12901 tcggtaacag cagttgatgg gcattagctt caagaatgac catcgaaatc tgctcttcgc
    12961 tctgacgcac gaaccatgca aactgctcat aggttacgcc ctgcataacg gataaagact
    13021 ggcaaaccac cagctttggc agattatcat cctgcatatc aagaaatgct ttcacggtca
    13081 gcgaactggc attgatggca gataaatccg ccgccagcgg caatacggct gaaggtcgga
    13141 cttccgccat ggcagaaaac aggatcgtgt tatcgatcag atcgattttg gcatcaaaga
    13201 caccgtcgaa attctgcata tggggcagat gcagagcctg acagttatca cattcaaaaa
    13261 aactcatccc caggtcatcg agccattgac gcagcgtatc cagaccagga acgaccagcg
    13321 atgtcataat tttgtacaac ctctttcgat aaaaagaccg gcacagctta cgcaaaaagc
    13381 gcaggcaaaa ccatgatcag taatgtgatt gcgactaacc acccgttttc aggcaatatt
    13441 ctgtcgtagc gtggcgttcg atccagcgga tcattttacc cgcgatgtcg ataccagtgg
    13501 ttttttctat tccttccagc cccggcgacg cattcacttc catcaccaac ggcccgcgat
    13561 tagcacgcag aatatccaca ccagcaacgt ccagcgccat cgttcgcgcg gcttttatcg
    13621 cgatttcacg ctcctgtggt gtgatactgg cgacgcttgc cgcgccgcca cgatgcaaat
    13681 tggaacgaaa atcgccctct ttcgcccgcc gttcaatcgc agcgaccact tcatcgccaa
    13741 caaccagaca gcggatatcg cacccttgcg cctctttgat atattcctgc accagaatat
    13801 gcgcgttcag accgcggaaa gcgtcaatca cgctttccgc cgcctgacgc gtctccgcca
    13861 gcacgacgcc aattccctgc gtgccttcaa ccaacttgac caccagcggc gcaccaccga
    13921 ccatgtcgat taaatcgctg gtatcatccg gcgaatgcgc aatgcccgtg acaggcaggt
    13981 cgatgccctg acgcgccagc agttgcatgg aacgcaattt gtcacgcgcc cgggcaatgg
    14041 cgaccgactc attgagcgga tagctcccca gcatctcgaa ctggcgcagt gccgccgtcc
    14101 cataaaaggt aatggcggtg ccaatacgcg ggatcactgc gtcaaaatgg ggtaacttgc
    14161 ggcctttgta gtgaatagaa gacgccgcag gatttatgtt catgtagcaa gaaagcggat
    14221 caagaatttc aaccaggtga ccgcgctgta tagcggcttc acgcagccgc ttacacgaat
    14281 agagcgttcc atcccgggac aatatggcaa ttttcaccct gcacctctct gtcagacctg
    14341 gtcaaaagcc cgcgtatcat acatcgacgt ggcggtttta gcgcgtcgcc caaccctgtt
    14401 tgtgcaaata atccagaata aatgggcggc tttctttaat gattgttcgg cggatatgat
    14461 cgctccaggt atcccggcga ttattgctgc cacgggtgag gtaatattcc gccagttgct
    14521 cgtcatactg cgccagtgcg tctttatcca gcggttgata gctgttttca tgcaccaaaa
    14581 tggaggccgg taaacgcggc ttgagatccg gattatccgc aggccagcca aggcacagcc
    14641 caaacagcgg cagaacatgc tgcggtaatt tcagcagttt cgtcaccgct tcaatattat
    14701 tgcgcaggcc gccgatatat accccg
//
LOCUS       DAZPJR010000100        14369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPJR010000100 DAZPJR010000000
VERSION     DAZPJR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.101"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..696)
                     /locus_tag="ACHVB7_002806"
     CDS             complement(<1..696)
                     /locus_tag="ACHVB7_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1914744.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="ACHVB7_002807"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="ACHVB7_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR1914745.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHVB7_002808"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHVB7_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR1914746.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="ACHVB7_002809"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="ACHVB7_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR1914747.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="ACHVB7_002810"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="ACHVB7_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR1914748.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="ACHVB7_002811"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="ACHVB7_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR1914749.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="ACHVB7_002812"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="ACHVB7_002812"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR1914750.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="ACHVB7_002813"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="ACHVB7_002813"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR1914751.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="ACHVB7_002814"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="ACHVB7_002814"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR1914752.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="ACHVB7_002815"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="ACHVB7_002815"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR1914753.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="ACHVB7_002816"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="ACHVB7_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR1914754.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHVB7_002817"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHVB7_002817"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR1914755.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /gene="acpS"
                     /locus_tag="ACHVB7_002818"
     CDS             10119..10499
                     /gene="acpS"
                     /locus_tag="ACHVB7_002818"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR1914756.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="ACHVB7_002819"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="ACHVB7_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR1914757.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHVB7_002820"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHVB7_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR1914758.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="ACHVB7_002821"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="ACHVB7_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..14032)
                     /locus_tag="ACHVB7_002822"
     CDS             complement(12845..14032)
                     /locus_tag="ACHVB7_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1914759.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14032..>14369)
                     /locus_tag="ACHVB7_002823"
     CDS             complement(14032..>14369)
                     /locus_tag="ACHVB7_002823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914760.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3621 a         3606 c         3708 g         3434 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gggggcatta tccatcaccc
    13321 gggatacgtt gacattccag ccacggcggt aatgcgtgtc caggaagcga ttccattccc
    13381 gtttactgct tccttctgct gccagcgcat ccggcatcac caggtcaggg tatttccgtg
    13441 acagcaaccg tgttatccgg tagcgccaca tgctcatcac cttacgggcg taaaaatgaa
    13501 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
    13561 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
    13621 ctgcctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgtcagta
    13681 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
    13741 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
    13801 ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt gtatggcaac
    13861 agtccggtga tgaacagcac cactgtgtat accccatcag tgtggtcccg cacgccatga
    13921 ttttggtcac cgactcaatc accaccggac gtactgccct ttccggctgc ttctccagcc
    13981 agttaagcca gtggtttccc tgctgaaaga tatcggcaaa acggggaagc atcagaaggg
    14041 cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca taccgccggc
    14101 gctgataccg gaaagaatgg tcgcaaattc ccgctccgtg cagcgggcga tttccggata
    14161 cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttcccg catccacaaa
    14221 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    14281 aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga catagcgggg
    14341 aagtttgctt ctttgagagg cgggtttac
//
LOCUS       DAZPJR010000101        14306 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPJR010000101 DAZPJR010000000
VERSION     DAZPJR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.102"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="ACHVB7_002824"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="ACHVB7_002824"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR1914761.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="ACHVB7_002825"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="ACHVB7_002825"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR1914762.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="ACHVB7_002826"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="ACHVB7_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR1914763.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="ACHVB7_002827"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="ACHVB7_002827"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR1914764.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="ACHVB7_002828"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="ACHVB7_002828"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR1914765.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="ACHVB7_002829"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="ACHVB7_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1914766.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="ACHVB7_002830"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="ACHVB7_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR1914767.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="ACHVB7_002831"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="ACHVB7_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR1914768.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="ACHVB7_002832"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="ACHVB7_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR1914769.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="ACHVB7_002833"
     CDS             complement(13987..>14306)
                     /locus_tag="ACHVB7_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1914770.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAZPJR010000102        14107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPJR010000102 DAZPJR010000000
VERSION     DAZPJR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.103"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(13..1371)
                     /gene="hprS"
                     /locus_tag="ACHVB7_002834"
     CDS             complement(13..1371)
                     /gene="hprS"
                     /locus_tag="ACHVB7_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR1914771.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="ACHVB7_002835"
     CDS             complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="ACHVB7_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR1914772.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2175..2588
                     /gene="hiuH"
                     /locus_tag="ACHVB7_002836"
     CDS             2175..2588
                     /gene="hiuH"
                     /locus_tag="ACHVB7_002836"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR1914773.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2697..3701
                     /gene="msrP"
                     /locus_tag="ACHVB7_002837"
     CDS             2697..3701
                     /gene="msrP"
                     /locus_tag="ACHVB7_002837"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR1914774.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3702..4337
                     /gene="msrQ"
                     /locus_tag="ACHVB7_002838"
     CDS             3702..4337
                     /gene="msrQ"
                     /locus_tag="ACHVB7_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR1914775.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4594..5244
                     /gene="zinT"
                     /locus_tag="ACHVB7_002839"
     CDS             4594..5244
                     /gene="zinT"
                     /locus_tag="ACHVB7_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR1914776.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5587..6117
                     /locus_tag="ACHVB7_002840"
                     /pseudo
     CDS             5587..6117
                     /locus_tag="ACHVB7_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6687..6776)
                     /locus_tag="ACHVB7_002841"
     tRNA            complement(6687..6776)
                     /locus_tag="ACHVB7_002841"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6740..6742),aa:Ser,seq:cga)
     gene            6870..7667
                     /gene="mtfA"
                     /locus_tag="ACHVB7_002842"
     CDS             6870..7667
                     /gene="mtfA"
                     /locus_tag="ACHVB7_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR1914777.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7768..7843
                     /locus_tag="ACHVB7_002843"
     tRNA            7768..7843
                     /locus_tag="ACHVB7_002843"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7801..7803,aa:Asn,seq:gtt)
     gene            8157..14107
                     /gene="yeeJ"
                     /locus_tag="ACHVB7_002844"
                     /pseudo
     CDS             8157..14107
                     /gene="yeeJ"
                     /locus_tag="ACHVB7_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3990 a         3353 c         3171 g         3593 t
ORIGIN      
        1 tgaagcccta tgttaatttc tttgcggtaa cataatccgg aacacattat gcttattgag
       61 atagtgatac gtagcacttc ccccatgtaa ttcggcaatc gctttgacta aagaaagacc
      121 aagtccctga cctacggaat gacgcgaatt atctccccgc caaaatctac ggaagagttt
      181 ttcaggctca tgaattttcg ttccaggact ggcgacatca atattaagat agccgttggt
      241 atcaagaaaa ctggttatat gaatacgcga tttttctggt gaatatctaa tggcattaac
      301 aataagattc gataacattc gttgcagtaa aattttatcc gcaaagattt gctgattgca
      361 ctcgacctta aagcaaatct ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt
      421 ttcgacttcc ttattgagag aaagcgagtc cagtttcacc aaaacattgt ttttatctgc
      481 ccgggcaaga aacagtatgt tctctgttaa ccgcgaaata ttttccagct cttcaatgtt
      541 accggcaatt gttttttgat attcagcgat acttctggtt tgactgagcg taacctgatt
      601 ctgacccagt aatgcattaa tgggcgttct aagttcatga gcgagatcgt cagcaaattg
      661 acttagacgc tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag
      721 aggttttagt tctctcggta atgcgctaac ctcaacaggc tcccggctat cgttataatt
      781 cagcgcttcc gttacaccac tcaacttttt gatctctcgt aatcccgttc tgattaacag
      841 cggacttaat actgagcaaa gtacaatggc gacaatgcaa attataatgc tattaatttt
      901 atactgttca agcatgttat gtctggctga agccaattta gccacagtaa ccgttaatga
      961 tggcgaaact tcatcaatat taattcgtaa agcatcaatc tcagtatcat taataatgct
     1021 tctgtaaatg ccagctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc
     1081 actgacattt gtccggttaa caattttatt gatgccatca ccatgaatga tcaagatatc
     1141 ctgactaaca tccatcatcc ggttaaagta tacaggtaac gtatctggat ttaccccatc
     1201 aattaacaac tgcttgatct gtgctgtccg gttaatgagt gttgtatcat cgcgccattt
     1261 caactcactt gccaggccat tatagagagt ccagacaatt ccagcgccag caacagacag
     1321 cagcaatata aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt
     1381 accgctacga atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga
     1441 tcatcaactt ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca
     1501 aagttgattc cccaaatttc actcgcaata accgttctgg gtataatttc gccagctctg
     1561 gaggccagta gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgttc
     1621 ctgctcacac tttgacttac agagtccatt cttaagccac tgatttctaa tgttgaattc
     1681 aaagcgtgat gttgccttaa ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa
     1741 ggttttacca gataatcatt tgctccactg tccagccctc tgactctgtc atcgacagaa
     1801 tcccttgcag taaggcaaat aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc
     1861 tgccagccat ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc
     1921 agcgcaagat aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg
     1981 gaaagcccct gcgttaccca ctcctgggtc ctttgattat cttcaataag taaaatcttc
     2041 atgaaaataa tatgccataa atcatatgtt atagaaacag cctagttcat tacaaaattg
     2101 taatgctgct gtaaggttac cctggccgct ttttcgctat cctcaaaact cattcacatg
     2161 acaaggatat aaacatgtta aagcgttatt tagtactctc cgtagtaacg gcagcatttt
     2221 cattaccttc tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc
     2281 agcaaaccgg aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggacaacg
     2341 gctggttaca acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc
     2401 ccgagcaaac tgcaactacg ggcgattacc gtgtcgtatt taaaaccggg gactatttca
     2461 agaaacaaaa tcttgaaagt ttcttccctg agatccccgt tgaatttcat attaataaag
     2521 tgaacgagca ttatcatgtg cctttattac ttagccaata tgggtattca acctatcgtg
     2581 gcagttaatg cataaatatg taaaagcaga tctctgctac tgttagggag gacactccct
     2641 gacagattaa cagtaaacgg ctcttgctgg ctaacgacaa aaaagtgtga tggcttatga
     2701 aaaagaatca atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc
     2761 gtcgacaggt gttaaaagca ctgggcatca gcgcagctgc actttctttg cctcacgctg
     2821 cgcatgccga tctgcttagc tggtttaaag ggaacgatcg cccgcccgcc cccgccggaa
     2881 aaccgctgga gttcagcaag cctaccgcct ggcaaaataa tctgccactg acgccagtag
     2941 ataaagtctc cggttataac aacttctatg aattcgggct ggataaagcc gatcccgccg
     3001 ctaatgctgg tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa
     3061 aaccattgac cctcgatcac gatgatttaa cccgtcgctt cccgctggaa gagcgtattt
     3121 atcgtatgcg ctgcgtggag gcatggtcga tggtggtgcc gtggattggt tttccgctgc
     3181 acaaattgct ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa
     3241 tttatgcacc ggaacaaatg ccaggccagc aggaccgctt tatcggcggc gggctgaaat
     3301 atccttatgt cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg
     3361 tgggtgttta tggcaaggcg ttaccgccac aaaatggcgc gccggtacga ctgattgtgc
     3421 cgtggaaata tggctttaaa gggattaaat cgatagtcag tattaagctg acccgcgagc
     3481 gtccgccaac cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta
     3541 atccgcatgt tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg
     3601 gcatcctcga tgttcagcgc cagccaacgc tactgtttaa tggttacgcc gaccaggtgg
     3661 catcgctgta tcgtggcctg gatttgcggg agaatttctg aatgcgtctg acagcaaaac
     3721 aggtgacatg gctgaaagtt agcctgcatc ttgccggatt gttgccgttt ctctggctgg
     3781 tctgggcgat caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta
     3841 ccggtcgcac tgcactgaaa tttttgctgg cgaccttgtt aatcacacct ctggcacgct
     3901 acgcaaaaca gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct
     3961 gggcgacact gcatttaacc agttacgcat tgctggagct gggcgtgaat aatctggcgt
     4021 tattaggcaa ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta
     4081 ttttgcttgc tttagcgttt acttcaaccc aggcgatgca gcgaaaactc ggcaagcact
     4141 ggcaacagtt gcataatttc gtctatcttg tcgcgatcct ggccccaata cattatctgt
     4201 ggtcagtgaa gattatttca ccgcagccgc tcatctacgc tgggctggct gtactgcttt
     4261 tagccttacg gtataagaag ttgcgttccc tttttaagcg gttacgcaag caggttcaca
     4321 acaaattatc tgtataactt catattgccg acaaagtacg cgttatccct gccggatgtg
     4381 gcgcgagcgc cctgtccggc ctgcgcagat cacatcaggt tatgtttcgt ttgtcatcaa
     4441 cctcaaatta tcaacataat tcattttatt tcaataagat agataagtac acctgagaaa
     4501 actatgctct catctcctaa gagttgttgc attttgccat atgttacaat ataacattac
     4561 acatcatata caatgactct ggaggaaact gttttggcaa ttcgtcttca caaactggct
     4621 gttgctttag gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcactcacac
     4681 ggcaaaccct taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgatgccaat
     4741 gtacaaaacc gaacgctcag tgactgggat ggagtctggc aatccgttta tcctttactg
     4801 caaagtggca aacttgaccc cgtctttcag aagaaagcgg atgcagataa aattaaaaca
     4861 tttgctgaaa ttaaagatta ttatcacaaa ggttatgcaa cagatatcga gatgattggc
     4921 attgaggacg gcattgttga attccataga aataatgaaa caacatcctg taaatatgat
     4981 tacgatggat acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc
     5041 gaatgtaaag atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt
     5101 gcaccacgaa aatcatctca ttttcacatt tttatgggta atgattcaca gcaattttta
     5161 ttaaatgaaa tggaaaactg gccaacgtat tatccatatc agttgagtag cgaagaagtg
     5221 gtcgaggaaa tgatgtctca ttgagtattc tcatgataac gcctcgatgc cgctttagta
     5281 agtcatcata actgccactg gtccatccac aaacgccact gaacgcaagc tagctacaga
     5341 cacgctcatc actatgacgt gtctgtatat taataagcta acccgcattg agttaaccaa
     5401 taacggattc catacacaat acggccaaca gagaaagata cctgtgctca cgccattgct
     5461 tatattggct ggttacaatg tgcactatca attttttaaa taacaaattc ttaaaaccac
     5521 atctgtataa ggaattttta aggttcgtgg gatagcttga ctgtaaaaat tacaggagct
     5581 acaaaaatga accgattctc aaaaactcaa atttatttac attggataac gctgcttttc
     5641 gttgcaataa cctatgccgc gatggaactc cgtggctggt ttcctaaagg tagtagtact
     5701 tatctgctga tgcgagaaac acattacaat gcgggtatat tcgtttgggt gttaatgttt
     5761 tcacgcctga ttataaaaca ccgttatagt gatccttcta ttgtaccacc gccacctgcc
     5821 tggcaaatga aagcggcttc gctaatgcac atcatgcttt atataacctt ccttgcactt
     5881 cctctgctgg ggattgcttt gatggcttac agtggaaaat cgtggagttt ccttggtttc
     5941 aatgtgtctc cctttgttac cccaaacagc gaaattaaag cactgataaa aaatattcac
     6001 gaaacctagg caaatatagg ttacttttta atcgcagctc acgctggcgc agcactcttt
     6061 catcactaca ttcagaaaga taatactctg ttacgaatga tgcatcgccg caaataagtc
     6121 gatcccctga caaggaaatg tgaaccatgt acatcaacgc gtcataaatg tttacgcaag
     6181 tgcacaatgc atcctttcta ctatcaacaa ggaggagcag aaactgtgta aatcgtacaa
     6241 tggtaagact tatcaaaaat acaggttaat tttggtattt aaaccttttt tacttgttag
     6301 tgtgatacaa aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact
     6361 ttgtcccgcg gctttaatct gacggccgcg ttcctttttt gactcaatta ttgacccctt
     6421 ctctacgcaa cttcagttcc caccaccaac tttgcggcag tttcatagga tgaatgtcta
     6481 aaagaataat ggtgagcgat aagaaaacga ctgaataact gcacattttc gctcgaaagc
     6541 ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt
     6601 gacgatctaa cccttcaaga atattctaca attgttctgt ttaggaaaag caaggcggga
     6661 agtcaggaga taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga
     6721 gttgccccta ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc
     6781 agatggttgc catgatgcca ggaaatttgg cattttaaca gtccctgttc gtgcaatttt
     6841 gttcaagtga cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat
     6901 cagcacatca aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgggtc
     6961 tgacggaaca ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc
     7021 ggcttgttcc tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc
     7081 tattttgcct acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct
     7141 taatttatcc tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc
     7201 ataaccaacg tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact
     7261 ggttggatat acaagattct tttgatgcat ctggttttaa cctgattatt catgaagtcg
     7321 ctcataagct ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc
     7381 gtgaggttgc tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa
     7441 tcgaattggt tggcgagaat gcggcgagca ttgatgctta tgcagccagc gatcctgctg
     7501 aatgttttgc cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt
     7561 tcccttcatt gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc
     7621 atcacgctaa tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc
     7681 agattaatta accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct
     7741 aagtaatatg tgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact
     7801 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct
     7861 tttttagcgc aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt
     7921 taacacctta agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac
     7981 cattttcccc tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca
     8041 cccaaaactg cctaacgaaa agttattaat tttcaatcgt attgctatca gtatttacat
     8101 tttttcgctg tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg
     8161 ctacgaagaa gagaagtgga gaagaaataa atgaccgaca aatattatgc gggatgggaa
     8221 ttaaactacg ccgcttaact gcgggtatct gtctgataac tcaacttgcg ttccctatgg
     8281 ctgcggcagc acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg
     8341 ccattgcaaa tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg
     8401 ttgccgaacg tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt
     8461 ttgctcgagg ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta
     8521 gtgaaaaaaa attaaccccg ccgccgggta atagcagtga caacctcgag caacagatag
     8581 ccagtacttc acagcaaatc gggtctctgc tcgccgaaga tatgaacagc gagcaagcgg
     8641 caaatatggc gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa
     8701 gccgcttcgg taccgcaaga atcacgctgg gcgtggatga agattttagc ctgaagaact
     8761 cccagttcga ttttctccat ccgtggtatg aaacgcctga taatctcttt ttcagtcagc
     8821 atactctcca tcgtactgac gagcgtacgc agattaacaa cggcttaggt tggcgtcatt
     8881 tcactcccac atggatgtcg ggcatcaact tctttttcga ccacgatctt agccgttacc
     8941 actcccgcgt cggcattggc gcggagtact ggcgcgacta tctaaaatta agcagtaacg
     9001 gctatttgcg actgaccaac tggcgcagcg cacctgaact ggacaacgat tataaagcac
     9061 gcccggccaa tggctgggat gtacgcgcag aaagctggct acccgcctgg ccgcaccttg
     9121 gcggtaaact ggtctatgaa cagtattatg gcgattaagt ggccctgttc gataaagacg
     9181 atcggcaaag taatcctcat gccataaccg ctggacttaa ctataccccc ttcccgctga
     9241 tgaccttcag cgcggagcaa cgccagggta aacagggcga aaatgacacc cgttttgccg
     9301 tcgattttac ctggcaacct ggcagcgcaa tgcagaaaca gcttgacccg aatgaagtcg
     9361 ctgcacggcg tagccttgca ggcagccgtt atgatctggt ggatcgcaac aacaatatcg
     9421 ttctggaata tcgcaaaaaa gaactggttc gcctaaccct gacagacccc gtgacaggga
     9481 agtcaggaga agtgaaatca ctggtttcgt cgctacaaac caaatatgcc ctgaaaggct
     9541 ataacgtcga agccaccgca ctggaagctg ccggtggcaa agtggtcaca acgggtaaag
     9601 atattctagt taccctgccg gcttaccggt tcaccagtac gccagaaacc gataacacct
     9661 ggccgattga agtcaccgcc gaagatgtca aaggcaattt gtcgaatcgt gaacagagca
     9721 tggtggtcgt tcaggcacct acgctaagcc agaaagattc ctcggtatcg ttaagtaccc
     9781 aaacattgaa cgcggattcc cattcaaccg ccacactgac ttttattgcg catgatgcag
     9841 caggtaatcc tgttgtcggg ctggtgctct cgacgcgtca cgaaggtgtt caggacatca
     9901 ccctttctga ctggaaagat aatggtgacg gaagctatac ccagatcctg accacaggag
     9961 cgatgtctgg cacgctgacg ctgatgccac agctgaatgg tgtggatgcg gctaaagccc
    10021 ccgccgtggt gaatatcatt tctgtttcgt catcccgaac tcactcgtca attaagattg
    10081 ataaggaccg ttatctctcc ggcaatccta tcgaggtgac ggtagaactg agagatgaaa
    10141 atgacaaacc tgttaaggaa caaaaacagc aactgaataa cgcagtcagc atcgacaacg
    10201 tgaaaccagg agtcactaca gactggaaag aaaccgcaga tggcgtctat aaggcgacct
    10261 ataccgccta taccaaaggc agtggactta ctgcgaagct attggaatga agatttgcat
    10321 accgctggat ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc
    10381 agcaataatg gtgtactcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct
    10441 gatgaaggaa gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg
    10501 gcaacttcct tcaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactttt
    10561 gatctgaaaa gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg
    10621 aaacaaacgt taatcgtcag ttttgtcggc gactcgagta ccgcgcaggt tgatctgcag
    10681 aagtcgaaaa atgaagtggt tgctgacggc aatgacagcg ccacaatgac cgcgaccgtc
    10741 cgggatgcaa aaggcaacct gctcaatgac gtcatggtca ctttcaatgt taattcagca
    10801 gaggcgaaac tgagccaaac cgaagtgaat agccacgacg ggatcgccac agctacgctg
    10861 accagtttga aaaatggtga ttatagggtt acggcctctg tgagctctgg ttcccaggct
    10921 aatcaacagg tgaattttat cggtgatcaa agtactgctg ccctgaccct cagtgtgcct
    10981 tcaggtgata tcaccgtcac caacacagct ccgcaacata tgactgcaac cttgcaggat
    11041 aaaaatggca acccactaaa agataaagaa atcaccttct ctgtgccaaa cgacgtcgca
    11101 agtaagttct cgattagcaa cggaggaaaa ggcatgacgg atagtaacgg ggttgcaatc
    11161 gcctccctga ccggcacgtt agcgggcacg catatgatca tggctcgtct ggctaacagc
    11221 aatgtcagcg atgcacagcc aatgacgttt gtggcggata aagacagagc ggttgtcgtt
    11281 ttgcaaacat cgaaagcgga aatcattggg aatggcgtgg atgagacaac tctgacagca
    11341 acagtgaaag atccgtcgaa tcatccggtg gcggggataa cggtaaactt caccatgcca
    11401 caggacgttg cggcaaactt tacccttgaa aataacggta ttgccatcac tcaggccaat
    11461 ggggaagcgc atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg
    11521 ctgggtaata acaataccag tgattcgcag ccggtaacat ttgtggcgga caaagcctcg
    11581 gctcaggttg tcctgcagat atcaaaagat gagatcacag gtaatggcgt cgatagcgca
    11641 acgctaactg caacggttaa agatcagttc gacaatgagg tgaataatct tccggtaaca
    11701 ttcagctcag cctcttcagg actcaccctg accccgggag taagtaatac caatgagtct
    11761 ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg agcagacggt cactgcatca
    11821 ctggctaata atggtgccag cgacaacaaa actgtgcatt ttattggcga cacagcggcg
    11881 gcaaaaatta tcgagttgac gcctgtccca gacagcataa tcgccggtac cccgcagaac
    11941 agctccggca gcgccatcac cgccacagtc gttgataata atggctttcc ggtgaaaggt
    12001 gtgactgtga acttcaccag cagaacaaac tctgccgaaa tgacgaatgg cggccaagcc
    12061 gtaacgaacg aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa
    12121 tcaggagcga gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc
    12181 acatcaatta atgtcaacgc tgatgcgtct acggcacatc tcacctggct acaggcactt
    12241 tttgatacag tctccgcagg cgagacaacc agtctgtata ttgaggtgaa ggataattac
    12301 ggcaacggtg tcccccagca tcaggtgacc ctcagcgttt caccaagtga aggcgtgacc
    12361 cccagtaata acgctatata tactaccaac cacgacggca atttttacgc aagctttacc
    12421 gctacaaaag ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatgcaa
    12481 caaacagtga cctatgtgcc gaacgtcacg aatgccgaaa tcacgctggc aacctcgaag
    12541 gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca
    12601 gagggcaatg cgatagccaa cactgaagta acatttactc tgccggaaga tgttaaggcg
    12661 aacttcacgc tgagcgatgg cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc
    12721 acgctgaaag gtacaaaagc aggcgctcat actgttacag catcgataac tggcggtaag
    12781 agtgagcagt tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt taatcttaac
    12841 gttaccgagg acaattttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg
    12901 actgatggaa acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat
    12961 gtgagcgcaa gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag
    13021 gctgaagtta cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac
    13081 aattatggtg tcagtgatac gaaacaggtg actttgattg ccgatgctgg taccgcaaca
    13141 ctagcctcct taacctctgt atactcattc gtcgtcagca cgaccgaagg cgcgaccatg
    13201 actgcaagcg tcactgacgc taacggcaac ccggtagaag gcataaaagt taatttccgc
    13261 ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcactgaa
    13321 attcttgtga caagcaccga agtcggactg aaaacagttt cagcctctct ggcagataaa
    13381 cctactgaag tcatatcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt
    13441 accagtctgg agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct
    13501 cacgtcaacg accagtttgg caacccgatt cttaatgaat ctgtaacatt cagtgcagag
    13561 ccaccagagc acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc
    13621 gaggtctcca tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat
    13681 ggagcctcac ttgagaaaca actggaggct attgatgaaa aactgacact ctcggcgtcc
    13741 agcccgctta tcggtgtcaa ttccccaaca ggtgcaactc tgacggcaac gctaacttct
    13801 gcaaatggca ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg
    13861 acgttaagtg gcggaaaagt gagaactaac tcttctggtc aggctccagt cgttctgacc
    13921 agcaataaag tcggtacata tacggtgact gcatcgttcc ataacggcgt aacaatacag
    13981 acacagacaa ccgtgaaagt cactggcaac tcaagcaccg cccatgttac tagctttatc
    14041 gctgatccat cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt
    14101 gaggatg
//
LOCUS       DAZPJR010000103        14091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPJR010000103 DAZPJR010000000
VERSION     DAZPJR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.104"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHVB7_002845"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHVB7_002845"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1914778.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHVB7_002846"
     CDS             2273..2662
                     /locus_tag="ACHVB7_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1914779.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHVB7_002847"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHVB7_002847"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR1914780.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHVB7_002848"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHVB7_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR1914781.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHVB7_002849"
     CDS             complement(4321..4521)
                     /locus_tag="ACHVB7_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR1914782.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHVB7_002850"
     CDS             4687..5232
                     /locus_tag="ACHVB7_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR1914783.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHVB7_002851"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHVB7_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHVB7_002852"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHVB7_002852"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR1914784.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHVB7_002853"
     CDS             complement(6529..7353)
                     /locus_tag="ACHVB7_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR1914785.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHVB7_002854"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHVB7_002854"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR1914786.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHVB7_002855"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHVB7_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR1914787.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHVB7_002856"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHVB7_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR1914788.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHVB7_002857"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHVB7_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR1914789.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHVB7_002858"
     CDS             complement(11316..11969)
                     /locus_tag="ACHVB7_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR1914790.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHVB7_002859"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHVB7_002859"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR1914791.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHVB7_002860"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHVB7_002860"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR1914792.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3433 a         3562 c         3737 g         3359 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg a
//
LOCUS       DAZPJR010000104        13906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPJR010000104 DAZPJR010000000
VERSION     DAZPJR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.105"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            244..1497
                     /gene="kch"
                     /locus_tag="ACHVB7_002861"
     CDS             244..1497
                     /gene="kch"
                     /locus_tag="ACHVB7_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR1914793.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1552..1725)
                     /locus_tag="ACHVB7_002862"
     CDS             complement(1552..1725)
                     /locus_tag="ACHVB7_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR1914794.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1868..3328
                     /gene="cls"
                     /locus_tag="ACHVB7_002863"
     CDS             1868..3328
                     /gene="cls"
                     /locus_tag="ACHVB7_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR1914795.1"
                     /translation="MTTVYTLVSWLSILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3363..3692
                     /locus_tag="ACHVB7_002864"
     CDS             3363..3692
                     /locus_tag="ACHVB7_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR1914796.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3745..4749)
                     /gene="oppF"
                     /locus_tag="ACHVB7_002865"
     CDS             complement(3745..4749)
                     /gene="oppF"
                     /locus_tag="ACHVB7_002865"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR1914797.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4746..5759)
                     /gene="oppD"
                     /locus_tag="ACHVB7_002866"
     CDS             complement(4746..5759)
                     /gene="oppD"
                     /locus_tag="ACHVB7_002866"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR1914798.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5771..6679)
                     /gene="oppC"
                     /locus_tag="ACHVB7_002867"
     CDS             complement(5771..6679)
                     /gene="oppC"
                     /locus_tag="ACHVB7_002867"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR1914799.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6694..7614)
                     /gene="oppB"
                     /locus_tag="ACHVB7_002868"
     CDS             complement(6694..7614)
                     /gene="oppB"
                     /locus_tag="ACHVB7_002868"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR1914800.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7700..9331)
                     /gene="oppA"
                     /locus_tag="ACHVB7_002869"
     CDS             complement(7700..9331)
                     /gene="oppA"
                     /locus_tag="ACHVB7_002869"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR1914801.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10069..10716)
                     /gene="ychE"
                     /locus_tag="ACHVB7_002870"
     CDS             complement(10069..10716)
                     /gene="ychE"
                     /locus_tag="ACHVB7_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR1914802.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11194..13869
                     /gene="adhE"
                     /locus_tag="ACHVB7_002871"
                     /gene_synonym="adhC"
     CDS             11194..13869
                     /gene="adhE"
                     /locus_tag="ACHVB7_002871"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR1914803.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3438 c         3229 g         3581 t
ORIGIN      
        1 tccttatagc cggtcagtgc attcactacc acaagcagat aataacttta tctgtatttc
       61 tttatatgat gtgagcatat tcaggaaaaa tgacctttcc tgaggtacag ttgctcgctc
      121 aaatcctttg attactgcgg ataaagtcat ctactattgt ttaacacatt cacgcgctgc
      181 atgtatgttc agaaaatgtg cacgctaaga taattaaaca aaattgtggt tacagaggta
      241 tcagtgagtc actgggctac attcaaacaa acagcaacaa atttatgggt gacccttcgg
      301 cacgacattc tcgcgctggc cgtcttttta aatggattgc ttatttttaa aacaatctat
      361 ggtatgtcgg tcaatttgct tgatattttc catatcaaag cattttcaga gttggatctc
      421 tccttgctgg caaacgcccc actatttatg ctcggcgtct ttcttgtcct gaactccatt
      481 ggcttactgt tccgggcaaa gctcgcatgg gcaatcagta tcattttgtt gttgatagcg
      541 ctaatttaca ccctgcattt ttatccctgg ctgaaattta gtattggatt ttgcattttt
      601 acgctggtgt ttttgctgat actgcgcaaa gacttctccc acagtagcgc cgcagccggg
      661 acaatttttg catttattag tttcacgaca ttactgtttt actcaaccta cggtgcgctt
      721 tatttaagcg aaggttttaa tccgcgaata gaaagtttga tgaccgcgtt ctatttttcg
      781 atagaaacca tgtcaaccgt cggctatggc gatattgtcc ctgtttctga atcagcacga
      841 ttgttcacta tttcggtcat tatttccggc attaccgttt ttgccacatc tatgacctca
      901 atttttggtc cgcttatccg cgggggattc aacaaacttg taaaaggaaa caatcataca
      961 atgcatcgta aagatcattt tattgtttgc ggacattcga ttctcgccat caatacgatc
     1021 ctgcaactga atcaacgcgg acaaaacgta acggttatca gcaacttgcc tgaagatgat
     1081 atcaagcaac ttgagcaacg cttaggcgat aacgctgatg ttatccccgg tgacagtaat
     1141 gacagttcag tattaaagaa agcgggaatc gatcgttgcc gggccattct ggcgctgagt
     1201 gataacgatg cagataacgc gtttgttgta ctctcggcaa aagatatgag cagtgatgtc
     1261 aaaacagttc tcgccgtcag tgatagcaaa aacctgaata agattaagat ggtacatccg
     1321 gatatcattc tctcaccgca actgtttggc agcgaaattc tggcgcgagt tttaaatggt
     1381 gaagagatta ataatgatat gctcgtttca atgttgttga actccggtca tggcattttc
     1441 agcgataacg atgaacaaga aacgaaagct gacagtaaag aatcagtgca aaaatagagt
     1501 ttaaagacga aagagtaaac ttattgggca ccgcaatggt gcccagtata tttagatatt
     1561 gtagatcgca aacaataacg agttacgctg tttgtttaga atgcacttcc tgatgctgtg
     1621 gatacgcata tttcggcgcg attgcccctc aagccaacgc gatttacgtc ggctagcctg
     1681 acgctgcatg cgccagcgcc ccacttccgt tctactccgc ttcatgttaa ctactctatg
     1741 caataacaga atggtcatta tacccccacc cccgacgctg accagtgctt ttcccgcgtt
     1801 tttatttgcc agatgaatcc atatgcgtaa actcataaca atgcgctttc aaaaggattt
     1861 ctaatctatg acaaccgttt atacgttggt gagttggttg tccattctgg gatactggtt
     1921 gctcattgca ggcgtaactt tacgcattct aatgaaacga cgcgcagttc cctccgcgat
     1981 ggcctggctg ttgattattt acattctgcc gttagtcgga attattgcct atcttgccgt
     2041 tggcgagctc catttaggca aacgccgcgc tgagcgcgcc agagcgatgt ggccttccac
     2101 cgcaaaatgg cttaacgacc ttaaagcctg taagcatatc ttcgccgaag aaaatagcag
     2161 tgtcgctgcg ccattattca agctttgcga gcgtcgtcag gggatcgctg gggtcaaagg
     2221 gaatcagcta caactgatga ccgagtcaga tgatgtgatg caggcgttaa tccgcgacat
     2281 ccagctcgcg cgccataata ttgagatggt gttttatatc tggcagcccg gcggcatggc
     2341 ggaccaggtg gctgaatcat taatggcggc tgcacggcgt ggtattcatt gccgattgat
     2401 gctcgactcc gccgggagtg tggctttttt ccgcagcccg tggcccgagc taatgcgtaa
     2461 tgccggtatt gaagtggtcg aagccttaaa ggtcaatctg atgcgtgtgt ttttacgccg
     2521 tatggacctg cgccaacatc gcaagatgat catgatcgat aattacatcg cgtacaccgg
     2581 cagcatgaat atggtcgatc ctcgctactt caaacaagat gcgggcgtag ggcaatggat
     2641 tgatctgatg gcgcgtatgg aaggccccat cgccaccgcg atggggatta tttattcctg
     2701 cgactgggag attgaaaccg gaaaacgtat tctgccgcca ccaccagatg tcaatattat
     2761 gccgtttgaa caggccagcg gtcacaccat tcacacaatt gcttctggcc ccggctttcc
     2821 ggaagatctc attcaccagg cattattgac tgcggcttat tcggcgcgtg aatatttgat
     2881 catgacaacg ccctactttg tgccaagcga tgatttactt catgcgattt gcacggcggc
     2941 gcagcgcggg gtggatgtca gtattatcct tccgcgaaaa aatgactcga tgctggtcgg
     3001 ctgggccagt cgcgcattct ttacggaact gcttgctgct ggggttaaaa tttatcagtt
     3061 tgaaggcggg ttactgcata ccaagagcgt gctggtcgat ggcgaactaa gtctggttgg
     3121 caccgttaac cttgatatgc gtagtctgtg gctgaatttc gagattaccc tggcaatcga
     3181 cgataaaggt tttggtgcag accttgccgc cgttcaggac gattatattt cgcgttcacg
     3241 tctgctcgat gcccgtttat ggctaaaacg tccattatgg caacgtgtcg ccgagcgact
     3301 gttttacttc ttcagcccgt tgctgtaaaa cgtcgccaac agaggttaaa caggtagtga
     3361 ctatggatat ggatctaaac aatcgcctga ctgaagatga aacgcttgag caggcctacg
     3421 atatttttct cgaactggct gccgacaatc tcgacccagc tgatgttctg ctgttcaatc
     3481 ttcagtttga agagcgcggc ggcgcggagt tattcgatcc ggcggaagac tggcaggaac
     3541 atgttgattt cgatttgaac ccggactttt ttgccgaagt ggtgattggt ctggcggaca
     3601 gtgaggatgg tgaaatcaac gatattttcg cccgcattct gttatgccgc gaaaaagatc
     3661 acaaactttg ccatatcatc tggcgggaat aaataatcgc tttcggcgtg aaaaagggct
     3721 gacaactgtc agcccttatt gtgtttaaag cggatcgact ttcaggcaag aaacggcgtg
     3781 tctgaaactc ccttccagca caggacgtgt tttggcgcac tccggaccgg caatcgggca
     3841 acaggtacgg aaaacacaac cggaaggcgg gttgatcggc gacggtaatt ccccttccag
     3901 taactggatg gttttgttct tctccagatc cggatcaggt atggggactg ccgacatcaa
     3961 tgccctggtg taaggatgta gtggattgtg gtagacctca tcataggtcc ccagttctac
     4021 cgcatggccg agatacatca ccaacacacg atcggaaatg tgttttacca cggccaggtc
     4081 atgagcgata aaaattaatg acaatcccat ctcacgttgc agctgctgga gcaggttgac
     4141 cacctgcgcc tgaattgaca cgtccagcgc cgaaatcggc tcatcgcaga taatcagctt
     4201 cggttcaaga ataagagcac gagcaatccc gatacgctgg cactgcccac cggagaactc
     4261 atgcggatag cggttaatca ggttaggcaa taacccgact ttcagcatca tcgccttcac
     4321 gcgctcgcga acttcctggc gtgacatttt cggatgataa gtacgcagtg gttcagcgat
     4381 gatctcgccg atggtcatac gcgggtttag cgatgccaac ggatcctgga aaatcatctg
     4441 aatatcactg cgaacggcac gccattcatc gggcttcatg cccagcaact ctttacctaa
     4501 ccaggcaaca tgaccgccgg tcgctttaac caggccaatg atggcgcgag caaaggtgga
     4561 cttaccgcat cccgattccc ctaccacacc taatgtttcc ccttcataca ggcgaagcgt
     4621 gacaccatcg acggctttga gagttttcgg cggttgccag aaccactgtt tgccatcttt
     4681 gatttcaaag tgcactttca gatcggcgat ttcgaggagg acttttcttc cttcggttac
     4741 agcattcata acagttcctc caccggttta aagcaagcac gcagacggcc aggcgtaaac
     4801 tcttccagcg gcggtgcgct actacaaatt tccatcgcat gcggacaacg tggctggaac
     4861 gggcaacctt tcggtaatcg cagcaggttt ggcggattac cagggatggt caacattgtt
     4921 tcaccttccg catcgagacg cggcaccgcg ttgagcaaac cgatagaata aggatgaacg
     4981 ggttgataaa agacatcgcg cgcgttgcca tattccatcg tgcgcccggc gtacattacc
     5041 agcactttgt cgcagatccc cgccaccacg ccaagatcgt gggtaatcat aatgatggcg
     5101 gtattaaatt cccgcttcag ttcatttaat agcgtcatga tctgcgcctg tacggtgacg
     5161 tccagcgcag tagtgggttc atccgcaatc agcagcttag gtcgacatag caatgccatc
     5221 gcaatcataa ctcgctgacg catgccacca gaaaattcgt gcgggtacat tttcatgcgt
     5281 ttacgtgctt ccggcatttt taccgcatcg agcatccgca ccgactcttc aaacgcctca
     5341 gctttgctca tgttcttatg cagcatcagc acttccatca actgctcacc gacgcgcata
     5401 tagggattca acgaagtcat tgggtcctga aaaatcattg agatttgttc agcgcgcagt
     5461 ttattgagtt cacgctctgg caaattgagg atttcacgcc cattgaaggt tgccgatccg
     5521 ccaatacgtc cgttggcagc caacaggccc atcaacgcaa atgcagtttg cgatttaccc
     5581 gaaccagact cacctacaat gcccagcgtt tctccggcac gtagagaaaa attcaaatca
     5641 ttgaccgccg tgacatcgcc gtccggggta ctaaaggtga cacgcaaatc tttcacgttc
     5701 agcagtacgt cagcctgttg ttgtgcgagc ggcacagttg cagtttcaat tacgctcatg
     5761 gctgcactcc ttaacgatct ttcgggtcga gagcatcacg caagccatcg ccgataaagt
     5821 tgaaacaaaa cagcgtcacc acgaggaatc ccgctgggaa caacaataac catggagaga
     5881 cttccatcga gttcgcgcca tcactcagca atgcacccca gctgcttaac ggctcttgcg
     5941 tacccaaccc caggaagcta aggaaagatt caaagaggat catgctgggc accagaagtg
     6001 atgcgtagac caccaccaca ccgagtacat tcggcacaat gtggcgaata acaatgcccg
     6061 atgtcgatac accgccaact tgtgccgcct caataaactc tttgcgcttc agactcaggg
     6121 tttgtccacg cacaatacga gccatatcca gccaggaaac catgccaatc gccacgaaaa
     6181 tcagcaggat gttttgaccg aaaaaggtca caagcaaaat gacgaagaac atgaatggga
     6241 aggagttgag gatttccagc agacgcatca ttaccgaatc cactttaccg cccagataac
     6301 cggaaagcga accgtaaagt gtccccacga ccactgccac cagtgccgca gcaacaccga
     6361 ccatgagtga gatacgcccg ccaatcgcaa cgcgcacaag caggtcgcga ccggatgagt
     6421 cagtaccaaa gtagtgaccg gactccatat ccggggcgct ggacatcatc gcccagtcag
     6481 tatcgtcata ggcaaactgc gaaagcatcg gtgccaggat tacaaataac gcgatcagca
     6541 ccagcactat cagactggca accgccgcac ggttatgcat aaaacgtcga cgtgcgtcct
     6601 gccacaagct gcgcccttcg acctccagct tttcactgaa attttccagc gtctcgctgt
     6661 ttttcttact taacatcatc gcgagctcca gtatcagtaa cggattttcg ggtcgataac
     6721 cgcatatagc acatcgacaa tggcattaaa caaaatggtt aaagcaccaa ccaggatggt
     6781 caggcttaac actaaggaat agtcacggtt caatgcgcca ttaacgaaca gttgtccaat
     6841 ccccggcaaa ccataaatgg tttcgataac catagaaccg gtaataatgc cgacaaatgc
     6901 ggggcccata taagagagca cgggtaacag agcaggtttt aatgcgtggc gtaaaatgat
     6961 ccgccgcata ggtaaccctt tcgcccgggc agtacgaata aagttggagt gtaatacttc
     7021 aatcatagag cctcgggtaa tacgcgcaat actggcgata taagcgagtg acaacgccac
     7081 catcggcaat atcatgaatt taagcgcccc accattccag ccaccgcccg gcagccaatg
     7141 caaaatgatc gcaaatatca tgactaataa tggcgcaacc acaaaactgg ggataacaac
     7201 cccggtcatt gccagcccca tcacggtata gtcccatttg gtgttttgtt ttaatgcgac
     7261 aataacgcca gcactaacac ccagtattac cgcaaggaaa aatgcggcgg ctcccagttt
     7321 ggcagaaacg ggaaaactgg atgcaaccag gtcattgacc gaataatctt tatatttaaa
     7381 cgatggaccg aaatcaccgt gcgccagttg tttcaggtag ctgaaatact gtgtcatgat
     7441 cggatcatta agatgatatt tcgcttcgat attggccatc acttccggcg gtaaagtacg
     7501 ttcgccggta aaagggcttc ccggcgcgag gcgcatcata aagaacgaaa tagtaataag
     7561 aataaatagc gtcggaatcg cttccagaca gcgacgtaga ataaatttta acattgccct
     7621 accttctggc ctgtgccttc tgtaatgcga taaaaatcag acaccgtgga gcaggacact
     7681 cctgccccac gtattgccat tagtgcttca caatgtacat attccgggtg taggtattat
     7741 ccagcggatc tttgccggta tagccaccaa cccacggttt caccagacgc gcattcacgt
     7801 agtaataaac aggaacaatg gccgaatcct tatccagctg ttgttctgct ttagtgtaca
     7861 gagctgtgcg ctgcgcctcg tcagtcactt tcagcgtttc cgccataatg ctgtcaaagg
     7921 ccgggctctt ataatgcgcg gtattcatcg agctgttcga cagcatggtg ttcaggaagg
     7981 aagttggttc gttgtagtct gcacaccagc ctgcacgggc cacatcaaaa gtgccctggt
     8041 gacgggtgtc gaggaacgtt ttccactcct ggttaaccag tttgacgttt acaccaatgt
     8101 ttttcttcca caatgaagag gcagcaatcg ccagcttttt atgcagatca gaggtgttat
     8161 acagcaggtt gatggtcaac ggtttgtctg cggtataacc cgcttcagcc agcagttttt
     8221 tcgcttcttc gttacgtttt tcctggctcc agccaaacca ttctggctga gtcaattttg
     8281 cgccatcagt atacggtgga gtgtaaccat aggcgggcat gtcgccctgc gctttcactt
     8341 tattaacaat gatatcgcgg tccataccta gtttcagtgc ggtacgcaca cgcacatcgt
     8401 tgaatggcgg tttctggttg ttaatttcgt aatagtaagt gcacaggtat ggatcaacgt
     8461 gaacttcgtc cgggatctct tttttcagct tctggaacaa ttcgatcggc atgttgttat
     8521 acgtcatgtc gatttcacca ctacggtagc ggttgacatc ggtaacttca gaagcaatag
     8581 gcaaataggt tacctggtta ataacggttt tcgcgttgtt ccagtaggtc gggctgcgtt
     8641 caagaacgat tcgttcgttt acgacccaat cttttaaggt ataggcaccg ttggtgacga
     8701 tattaccagg ctgggtccat ttttcgccga atttctcgat agcggctttt ggcaccggtg
     8761 aagttgatgg gtgaacaagt aatttataga agtacggaac gggttcactt aaggtgactt
     8821 ctaatgtgtg atcatcaata gctttcacgc cgagatcggt aatcggtttt ttcccttcaa
     8881 gaatttcatc aataccggcg atatgcccat attgcagata actggcatac ggagaagcag
     8941 tgttcggatc aacagaacgt tgccagctat acacaaagtc ttgtgctgtg actggcgtgc
     9001 tatcagacca tttcgcatct ttacgcaaat ggaaggtcca gactttcgcg tctttattat
     9061 cccaggattc agcgacgcca ggtgctggat gaccgtcaag atcgctgacc agtaagcctt
     9121 caaacaggtc tcggctgata ttagactccg gaacaccttc aattttgtgc ggatctaatg
     9181 actgaacttc tgaaccattg ttacgtacca gtgtttgttt ttccgccagt gtgacgcctg
     9241 cgggtacatc agctgccagc gcgacattcc ctgccattag cgcagccaga acgccagctg
     9301 ctactaaact tctcttggtg atgttggtca ttgttttttg gactccctca ttataattac
     9361 tggtattaac cagcatgtgt aatcccggat tgggaccctg cacaggctcg gagactttct
     9421 gctgtcaggt tactttatcg ttacgtatta ctatcaccga ctttatttat tctggtgatt
     9481 cagacgatca ccttatgtcg attcgtactg tgtctcccct gtcgagacac attattcttt
     9541 cgcgacgctc tttaattgtt aattattctc attaccaata aagaatcgtc tggcggttgc
     9601 ccggaaagta ccaaatgcgg ttcttactcg ccaatacatt ttgcaaaatt gttaagcaat
     9661 tctcttttat tgaccgtatc agaatctata gcttgcatcc atgccttaac aaacatcttt
     9721 caattcaatc aaatagagat tggctaagca tgtctatctt ttctgattac agttccagta
     9781 gtgaaatgca caataattta acaattgatt attatttagc actttcctct accaaaggca
     9841 gtggaattac taatatcatt tcaattattc ttcagcaagc ccaggattat gatgtggcga
     9901 aaataacatg atggtgagga gaagcagtta aggaaatcct tataactaac tgtaatttga
     9961 gaagaatatt tttacctatt ccttttgctt ttgataactg cgcgggaaca aagtaaaaat
    10021 aatgacgaag taaacggagc atggcagctc cgtttcattt gaaaggaatt aattaagcag
    10081 gccggggaaa atccccttaa taccagtaac gataaattca atccccaatg ccatcagcaa
    10141 tagccccata atacgcgtaa tcacgttgat gccggtctgg cgtaaaaccc gtaccagcca
    10201 cggtgccatg cggaacaatc cccagcaaca taaagcgaac aatgcaatag ccacaaagaa
    10261 accaaacaga tagctaatgc tgtgataacg cgtaccccag acgatggtag aactgatcgc
    10321 ccctggcccc gccatcaacg gcaacgccaa tggcaccaca ccaatgcttt cacgtaccgc
    10381 ggtttctgat ttttcttgct tgttctgttt atcctcgcca agcttgccgc tgatcatcga
    10441 catcgctatc gtcaccacca ggatgccccc tgcgatacgg aacgaatcaa ttgatatacc
    10501 aaaaagttgc agaatcgtgt cgccgagaaa aagcgagatc cacaagataa tggccacaga
    10561 caggttggct gtaaggttag ttttgtttcg cgctgccgct gtctgataac tggtcatgct
    10621 gataaagacg ggaataatcc ctaccgggtt gaccagcgca aataacccga tgaaaaattt
    10681 gaagtaaacg ggaaaatcaa aaaaggtctg aatcacggtt agctccgaag caaaagccgg
    10741 ataatgttag ccataaatta aggttgaaaa gacgcgctga caatacgcct tttgacagca
    10801 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
    10861 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
    10921 aaaagcgatg ctgaaaggtg tcagcttagc aaaaatttga tttggatcac gtaatcagta
    10981 cccagaagtg agtaatcttg cttacgccac ctagaagtga cgcattagag ataataactc
    11041 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
    11101 tcataggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
    11161 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
    11221 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
    11281 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
    11341 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
    11401 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
    11461 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
    11521 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagtctgaag
    11581 acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgccac caacaaagcg
    11641 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
    11701 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
    11761 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
    11821 atcggtgtag gcgcgggcaa cactccagtt gttatcgatg aaactgctga tatcaaacgt
    11881 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
    11941 cagtctgttg ttgttgttga ctctgtttat gacgctgtac gtgaacgttt tgcaacccac
    12001 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaac
    12061 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
    12121 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
    12181 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
    12241 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
    12301 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
    12361 aaaacggctc gtatcctgat taacacccca gcgtctcagg gtggtatcgg tgacctgtat
    12421 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggggg taactccatc
    12481 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
    12541 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
    12601 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
    12661 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
    12721 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
    12781 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
    12841 gacgccgcga agattatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
    12901 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
    12961 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
    13021 gttgtaactg acgacgctac tggtcagaaa tatccgctgg cagactatgc gctgactccg
    13081 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
    13141 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
    13201 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
    13261 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
    13321 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
    13381 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
    13441 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
    13501 caggctcgcc gtcgttatgc tgaaattgcc gaccacttgg gtctgagcgc accgggcgac
    13561 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
    13621 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
    13681 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
    13741 ctgatctccg agctgaaaca gatcctgctg gatacctact acggtcgtga ttatgtagaa
    13801 ggtgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
    13861 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttct
//
LOCUS       DAZPJR010000105        13807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPJR010000105 DAZPJR010000000
VERSION     DAZPJR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.106"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHVB7_002872"
     CDS             complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHVB7_002872"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR1914804.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHVB7_002873"
     CDS             1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHVB7_002873"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR1914805.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHVB7_002874"
     CDS             1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHVB7_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR1914806.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHVB7_002875"
     CDS             complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHVB7_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR1914807.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4201..5439
                     /locus_tag="ACHVB7_002876"
                     /pseudo
     CDS             4201..5439
                     /locus_tag="ACHVB7_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHVB7_002877"
     CDS             complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHVB7_002877"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR1914808.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHVB7_002878"
     CDS             complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHVB7_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR1914809.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHVB7_002879"
     CDS             complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHVB7_002879"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR1914810.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHVB7_002880"
     CDS             complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHVB7_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR1914811.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHVB7_002881"
     CDS             complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHVB7_002881"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR1914812.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHVB7_002882"
     CDS             complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHVB7_002882"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR1914813.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHVB7_002883"
                     /pseudo
     CDS             complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHVB7_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHVB7_002884"
     CDS             complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHVB7_002884"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR1914814.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHVB7_002885"
     CDS             complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHVB7_002885"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR1914815.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHVB7_002886"
     CDS             complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHVB7_002886"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR1914816.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3390 a         3497 c         3673 g         3247 t
ORIGIN      
        1 atcaataagt tggagtcatt acccccataa tgatattaag cgttttctga tcgggcaacc
       61 ccacggcctg ttgcagcgta ttttccttac tcatgtaata gatagccggc gtgacatttg
      121 cccccagatc gtccatcagt ttctcattgt cacttaacac tttcatttgc tctgtactta
      181 cgtttgcagg cacgtttagc ttaagcttgc caccagaggc ttcatattgt tgccaggttt
      241 ttgcgggatc tttggaggca agaattgccg ctgctgtcgc cgggctttct ggcttgataa
      301 ccccaaccaa caatgttctt aattgcactt tgccagaatc tacccacggg cgcgcctgct
      361 gccagaactg tttacaatat gggcagaacg gatcggcgaa gacgtagaca atgaccggcg
      421 catctttttt accgtcgagg atccagtggg attgttccat ccgttgccac atttcgcgtc
      481 cggctggtgc gtaaatttct ttttcgataa gtgtgttact caggttttca cctttctcgt
      541 tgtacatgta accagagata gcgtgcttac catcgggagt caggtagatg gtgacgccca
      601 tatcctgata ctttccgaga taacctttca ttcctccggg ggcatcgaac gttttgatga
      661 ttgtaatgcc ctgtttttca atcgctttta ctggggaagg aagttcctct gcgaaggcga
      721 ttgcaggaag cagagccagt aaaagtatct tttttaacat taatttgtcc ttttcagtca
      781 gtgcaaaagt cgagtaaaag gcataaccta tcactgtcat aggtaagagc ttagatcagg
      841 tgattgccct ttgtttatga gggtgttgta atccatgtcg ttgttgcatt tgtaagggca
      901 acacctcagc ctgcaggcag gcgctgaaga taccaaaggg tagttcagat tacacggtca
      961 cctggaaagg gggccatttt actttttatc gccgctggcg gtgcaaagtt cacaaagttg
     1021 tcttacgaag gttgtaaggt aaaacttatc gatttgataa tggaaacgca ttagccgaat
     1081 cggcaaaaat tggttacctt acatctcatc gaaaacacgg aggaagtata gatgtccttg
     1141 attaacacca aaattaaacc ttttaaaaac caggcattca aaaacggcga attcatcgaa
     1201 atcaccgaaa aagataccga aggccgctgg agcgtcttct tcttctaccc ggctgacttt
     1261 actttcgtat gcccgaccga actgggtgac gttgctgacc actacgaaga actgcagaaa
     1321 ctgggcgtag acgtatacgc agtatctacc gatactcact tcacccacaa agcatggcac
     1381 agcagctctg aaaccatcgc taaaatcaaa tatgcgatga tcggcgaccc gactggcgcc
     1441 ctgacccgta acttcgacaa catgcgtgaa gatgaaggtc tggctgaccg tgcgaccttc
     1501 gttgttgacc cgcagggtat catccaggca atcgaagtta ccgctgaagg cattggccgt
     1561 gacgcgtctg atctgctgcg taaaatcaaa gcagctcagt acgtagcttc tcacccaggt
     1621 gaagtttgcc cggctaaatg gaaagaaggt gaagcaactc tggctccgtc tctggacctg
     1681 gttggtaaaa tctaaatttc cttcgtcttt cgcgctacca gctccggcgc gaaatacttc
     1741 ggaaattcac ctaattcttc gggtgctgcg gcacccgatt ttcttccccg caccatgatg
     1801 caagctgcat ccaggtagcc gtagaggccg cttgcatgat gatgtttaaa gcccaggaga
     1861 taaacatgct cgacacaaat atgaaaactc aactcaaggc ttaccttgag aaattgacca
     1921 agcctgttga gttaattgcc acgctggatg acagcgctaa atcggcagaa atcaaggaac
     1981 tgttggctga aatcgcagaa ctgtcagaca aagtcacctt taaagaagat aacagcttgc
     2041 cggtgcgtaa gccgtctttc ctgatcacca acccaggttc caaccagggg ccacgttttg
     2101 caggctcccc gctgggccac gagttcacct cgctggtact ggcgttgctg tggaccggtg
     2161 gtcatccgtc gaaagaagcg cagtctctgc tggagcagat tcgccatatt gacggtgatt
     2221 ttgaattcga aacctattac tcgctctctt gccataactg cccggacgtg gtgcaggcgc
     2281 tgaacctgat gagcgtactg aacccgcgca tcaagcacac tgcaattgac ggcggcacct
     2341 tccagaacga aatcaccgat cgcaacgtga tgggcgttcc ggcagtgttc gtaaacggga
     2401 aagagtttgg tcagggccgc atgacgttga ctgaaatcgt tgccaaaatt gatacgggcg
     2461 cggaaaaacg tgcggcagaa gagctgaaca agcgtgatgc ttatgacgta ttaatcgtcg
     2521 gttccggccc ggcgggtgca gcggcagcaa tttactccgc acgtaaaggc atccgtaccg
     2581 gtctgatggg cgaacgtttt ggtggtcaga tcctcgatac cgttgatatc gaaaactaca
     2641 tttctgtacc gaagactgaa gggcagaagc tggcaggcgc actgaaagtt cacgttgatg
     2701 aatacgacgt tgatgtgatc gacagccaga gcgccagcaa actgatccca gcagcagttg
     2761 aaggtggtct gcatcagatt gaaacagctt ctggcgcggt actgaaagca cgcagcatta
     2821 tcgtggcgac cggtgcaaaa tggcgcaaca tgaacgttcc gggcgaagat cagtatcgca
     2881 ccaaaggcgt gacctactgc ccgcactgcg acggcccgct gtttaaaggt aaacgcgtag
     2941 cggttatcgg cggcggtaac tccggcgtgg aagcggcaat tgacctggcg ggtatcgttg
     3001 agcacgtaac gctgctggaa tttgcgccag aaatgaaagc cgaccaggtt ctgcaggaca
     3061 aactgcgcag cctgaaaaac gtcgacatta ttctgaatgc gcaaaccacg gaagtgaaag
     3121 gcgacggcag caaagtcgtt ggtctggaat atcgagatcg tgtcagcggc gatattcaca
     3181 acatcgaact ggccggtatt ttcgtgcaga ttggtctgct gccgaacacc aactggctcg
     3241 aaggcgcagt cgaacgtaac cgcatgggtg agattatcat tgatgcgaaa tgcgaaacca
     3301 acgtcaaagg cgtattcgca gcgggtgact gtacgacggt tccgtacaag cagatcatca
     3361 tcgccactgg cgaaggtgcc aaagcctctt tgagtgcttt tgactacctg attcgcacca
     3421 aaactgcata agaagaagta agattcacct gcaattgctt agccgccggg gtcaaacctg
     3481 gcggcttttt tatggcatta aaaagccctg ccgggatgtt ccaggcaggg cggcataaat
     3541 acttttatct gttagcgaat gaccagtaca ggcagattgg cgtggcggat cacgctcgag
     3601 gcgttagaac ctaacagatg ggtcgaaatc gatgggttgc gagaaccgat aactacaaca
     3661 tcagccccca gttcttctgc caactcattg acttcatccc gcacgctacc aaaacggaca
     3721 tgttgtttaa tgcgggaagg atcgatggtg aagtggctga ccatcgtttg cagacgttct
     3781 tctgcttcat gttgcagatg ctcttcaaaa cgacgcacat cagcggcaaa acggtgcagg
     3841 ctcaggctgg ctgacccggg tagtacgtga agtagatgaa taactccgtc atcctgggcg
     3901 aggaattcag cgtggcgaac agctttgtcg ctcaattcca tttcaaatac atcaactggc
     3961 ataatgattg tcttatacat aaccctttct ccctgttaat catgaacaaa tcattcgcca
     4021 tgattataat atttatccct gatatttgtc tggttctttt tccttacgaa cggtttctgt
     4081 gattaatata ttctcactga acaccaggaa ttctcccaaa acctgcggta ccgcccgttt
     4141 tcccgctgtg atagctaccc ttaaagactg actctttttt gaactgtctc tggaggttgc
     4201 atgaaagcat tgacttatca cggcccacat cacgttcagg tagaaaatgt tcccgatccg
     4261 ggcattgaac aggctgatga tattattctg cgtattacgg caacggcgat ctgtggctct
     4321 gacctccatc tttatcgagg caaaataccc taggttaaac atggcgatat tttgggtcat
     4381 gaatttatgg gggaagtcgt tgaaaccgga aaggacgtaa aaaatttgca aaaaggcgac
     4441 cgggtggtaa ttccgtttgt cattgcttgt ggcgactgtt ttttctgtcg aatgcagcaa
     4501 tatgccgcct gcgaaaatac caatgcgggt aaaggcgctg cgctcaataa aaaacagata
     4561 ccagctcccg cggcattgtt tggttatagt cacctgtatg gtggcgttcc tggtggacag
     4621 gcggaatatg tccgcgtccc taaagggaat gtggggccgt ttaaagtacc gcctttgctt
     4681 tcagatgata aagccctttt cctttctgat attctgccaa cggcatggca ggcagcaaaa
     4741 aatgcgcaga tccaacaagg ttcaagcgtt gcagtctatg gtgccggtcc agtgggattg
     4801 ttgacaatcg cctgtgcacg gttgctcggt gcggaacaga tttttgttgt tgatcatcat
     4861 ccctaccgct tgcgtttcgc cgctgaccgc tacggcgcga tcccgattaa tttcgatgaa
     4921 gatagcgatc cggcacagtc aattattgaa caaacggcag gtcaccgggg cgtggatgca
     4981 gtaatagacg ccgtcggttt tgaagcgaaa ggcagcacca cggaaacggt gctgactaac
     5041 ctgaaactgg agggcagcag cggtaaagcg ttgcgccagt gtattgcggc ggtcaggcgt
     5101 ggcggcattg ttagcgtacc gggcgtctac gctggattta ttcacggttt cctgtttggc
     5161 gacgcctttg ataaagggtt gacgtttaaa atgggacaga cccacgttca cgcatggctg
     5221 ggtgaattat taccgttaat tgagaaagga ttactgaaac cagaagaaat tgttacccac
     5281 tatatgccgt ttgaagaggc cgcccgggga tatgagattt tcgaaaaacg tgaagaggag
     5341 tgccgtaagg tgattctggt acccggtgca caaagcgcag aggcggcgca gaaggcggtt
     5401 tcaggtctgg tgaatgcgat gccgggggga acaatatgat cgtcaggagt ggttttcgag
     5461 gtaaaggaca gccatgacga taatcgccgc cataatcaga aatcctatca ggatgtaaaa
     5521 tgcttctgcc atggttattc ccacaaacga aacggaataa ttttgcagca aaagtgaaca
     5581 gtgagaacca ggaaaaattg ctgattttgc gtaaagagga tgcgagtgca tcctctgggc
     5641 aaagcgagtt atcgcttgtg cagatgggat taaagcaggt agtcgccagc agcttctggc
     5701 tggtattcga gttccagcac ttcaaggtgg gttgcaacgc cgcccggaag ttcccagtga
     5761 atggaatcgc caacgcgcag ccccagcagt gcggcaccta ccggagccat aacggaaagc
     5821 tgagtattgc tatcggtcat ttttgccgga tacaccagcg tgcgcacacg cacttcgcca
     5881 tcgctaagat tgcggaattt aacccggctg ttcattgtca ccacgtcgtg tggcatctct
     5941 tctggcgaac acatttgggc gcgatccaac tctgcgttta acgcgtcggc gattggcaaa
     6001 ccagcatagg cgggttgctc cagcagaata tcgatgcgtt cggcatccag gtcgttaatg
     6061 atgatagttg gtctggacat ttttactcca tgtcgtcggt actgcgagtg tcgcagataa
     6121 acatacccaa aagaaaaccc tcaccgtcag gcggcgaggg tttaactcac atgatgatac
     6181 tgactgttgc tcactctttg aagtgatttg cgtcacattc agggaattcc tcaatgcagc
     6241 gcattatgta taattcttaa tcgtcacggt ttatggaaga cgaattagcg tgttttgtaa
     6301 atcagatgat taataaccca ctttatcaat cacaaagttt ttgccacagt tgcctgggtg
     6361 tggttgtggc aagaatgagt ttgcagaaag cggagcgttg atggcgtcag ctttgatcga
     6421 gatctgaatt tcagtcaaat acgcagggtt accctggcac gttagcttaa ctgctttcac
     6481 gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc tcactgtttt
     6541 accgtaatta tccgcaagga attttccggc ttcgctctct ttaatttctt gattcaggcg
     6601 taccatcgta ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt gtttggcata
     6661 ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc atgacttcac ttagtttagc
     6721 ggccgtttcc agtgataacc cggtttccgg cgatgaacac atccggctgg cgtgcgcttc
     6781 tggtagattc gggattgggc gagtagcgca accgaagcgc atccagcggc gttcatcaac
     6841 accacgggca gcaaccgatt taggcaatcc tggccacaga ccatgtacgg tcagaaaatc
     6901 agctttgttg gtcgtttcgg tttgtaggcg acattcatct cgttcgttac gatttcgatc
     6961 gtgttgactc tggcaaaatc cggtttgcca ggagagggcc aggacatagc gatcaaaatc
     7021 gccatactgt tttgcctgca acgctacggc gttggcagaa gagaagggaa gcagagaaac
     7081 cgcgagcaac gcggcgttac gccagaatgc tttcataatg ggtgtggaac tcatacatac
     7141 actgaatact atctattaaa tcataaaaaa gcccgccagg tgggcttacc tggcgggcgt
     7201 gatgatttat tcagcgtttg gcgaacgtat tagttccaca tggcgagaat cggccagcca
     7261 accaacagca acatagagat gtaaatcacc ccgaagattg cgccaagacg ccagtaatct
     7321 tttgatttca catagccaca gccgtaaata atcaccccag gaccggttgc atacggcgtc
     7381 agacagccca tgataccgat agacagcacc agcaggatac acagttgttc cattggtacg
     7441 cccggaatac ctttaccgac ggccagaata accgacagca tggttgcggt gtgcgcagac
     7501 aggctggcaa acaggtagtg tgcaaagtag aacaccagaa ccagtacaat caccgttgcg
     7561 tttggtgaga atccttccag gtgcgtactc atggtaccgg cgaaccagtc aataaagcca
     7621 gaacgagtca ggccgttagc catcacaacc agagttgcca ggttgaccag cgtgttccat
     7681 gcgctgttat agcgggtaat atctttccaa ggcacaacgt gcagggccag cattagcgaa
     7741 actgccagca gaccaactgc agtagcatta atgacttcac tgccaaaaac ccacaaacct
     7801 aagctgagca atacaaggcc aatcagtgtc cactctctgc gtgtcagcgc acccatggtt
     7861 ttcagttcat cacccgccca ggttgccact tcttcactgt gtgtgatttc cggtttgtac
     7921 agcacgtagg aaagccacgg cgcaatgata agcaagataa ccccaaccgg caggaagcag
     7981 aggaaccact gcaaccagct aatctggata ccggcaattt tgctgacgaa ctccagaccc
     8041 agcacgtttg gtgccgcacc ggtgacaaac atggacgaac tcagactggt actaatgacc
     8101 atcatccaca tcaaatagcc gccaatacga cgcgcggacg gatcgttggg gaatgattta
     8161 aacagcggcg gcaggttttt aatgaccgga aaaaccgtac ccccggtacg cgcggtgttg
     8221 gacggtgtaa acggtgccag cagaatgtcg ataatgacaa tcgcataacc caacgtcagc
     8281 gtgcgtttgc ccatgaattt caccaggaaa agggcaatgc gacgacctaa cccggaaact
     8341 tcatacccta atgcaaaaat aaatgcgcca aataccagcc ataccgtggt gctggaaaaa
     8401 ccagccagac cccatttcag cgcctgtttt tgcgcattaa acgctgggtc agctaattct
     8461 ttggcatcaa agagcaggta attactgcca ataacgcaaa tagtaaccgc aataaaactg
     8521 atcgctgttg ccggaattgg ctcgaggatc atgccgacaa tcattgccac aaacacagcg
     8581 aagtaatgcc atgcctgtgg tggcataccg tcggggacag ggataagaaa catgacaccc
     8641 atcaccacca gtggggccaa tagtttccat atattatctt ttgctaaaga catacgggtt
     8701 ctccgaaaat taatatttcc aaatttatca agtgcttaaa taattaaatc tgtgctaaaa
     8761 accaggtaag gatcagcagg tcggcactgc cgcctggact gagatttcgt tcgatacact
     8821 ccctgtcgaa ctgccggaga taatcgagat cggcgggggt tcgaatgccc cctttttgca
     8881 ataatgtttg cgcctcgcgc tgtagccagc gcaggccccc ctcgccaccg cgcgatgcaa
     8941 cgttggtatc gccgttgatc gccatcagta ggagcaaggt atcgagcaat gccagttcag
     9001 gatctaaccc ctgatccagc agagtgaggt aatgcggcaa ggcgtgattg atcaccagtg
     9061 gataacccgc ttcggcttca ccgcgtgcgc cggtaaggcc aagctgctgg tacaaccgtt
     9121 gacctgccgt cagttgtgaa ttattggtac gcagttctcg atcggtcagg ccacggcaga
     9181 aacttgccgc cgtagaacaa acggttgttg gcgttaccgg ttggttgagt tgaagcaaac
     9241 ggccaattgc cgcacatagt agccctaaag aaaaaatgct gcctttatgc gtgtttacgc
     9301 ccgcagtggc gcggaacata tcaccttcgc aagccatacc aattgggcgt aatccgtgga
     9361 gtaccgcttc tggtgctatt tccgcactac aggcaccaaa ttcaatgaaa cggggtagcc
     9421 agccctgaat cgccagcgcg ctgcggtgga aatcttccag cgccatatct ttatgtgcac
     9481 cgcagttaat ccgatccacg aggcctggtt tcggtgacag attgacttca gtcagcatgg
     9541 cgcgccagcc cagcagggcg tactcatcga tcaatgacgt cgcaagcttt gtggttttag
     9601 ttgacgttgc aggcatcgac atcgttcagc agtgcctcca tgcggttgag taaatcggtc
     9661 agttgatggg tttttccacg cgcgcagacg gctgcgcttt gctcgcacaa caggcagcgg
     9721 cgaggcggca gtgaatagtc gcggcgggag agaatttcgc cttcgggcgt caggacatcg
     9781 atatcccata accgcccgag aggatgacta tgttcaagct caatggtggc gagcttgagg
     9841 tcgcgagccg gggcggcaat gctcaacatg ccctccggcc cgctggcgga aaccagtgca
     9901 gcctgctcct gaatttgcca gccctgtttt gcggctaagg cacgcaaggc tgtcacgcca
     9961 tgattaaaaa ttcggcgtgt gacctcgctg tctttaatcg gcccaggcgc aaccacggta
    10021 aaggagacca gtggaacagg atggcgcttg agccagacgt gttgccgtgc ttgcctttca
    10081 tcccggctga cgagcagctc gggaattgac accgcatggt ggctggcgag ttcaggaagc
    10141 aggtgcatgg cttattcctt cacctgatgc acaacatcga tcaccgagcc atcgcggtaa
    10201 cgcacaacgg caacgacgcg gtcggtgaat tcaatcggct gtggttcacc ggtcagcaga
    10261 cgcgcgcgtt cgcgcagcca ctcaatggaa accactttaa tgcccgcttc ctgcagacgt
    10321 tctgccagtt ccggacgtgc cgggttaact gcgataccgt ggtctgtgac cagaatatcg
    10381 acactggagc ctggggtgat gcaggtcagc acgttatcca ccagagtcgg aatacgaccg
    10441 cgtaccagcg gcgcgacgat gatggaaagc gcagaggcaa tcgcggtatc cagtgaccac
    10501 cggaagcacc acgcagtacg ccgtcagagc cggtcagcac gttaacgttg aactgggtgt
    10561 caatttccag cgcgctcagt accaccacgt cgagacgatc aaccgatgcg cctttcgaac
    10621 cccagttagc gtactggttg gcgctgattt cgatgtgatt ggggttacgg gccagcgatt
    10681 gcgcagcatg gctgtcaaag ctctgcacat ccagcagttt gcggatcaga cctttttcgt
    10741 gcagatcaac catcgtcgcg gtaataccgc cgagggcgaa gtcggcgcga atatcgcggc
    10801 tacgcatttt gtcttccagg aaacgggtta ccgccagcga tgcgccgccg gtgccggttt
    10861 gcatggagaa accttctttg aagtagccag agttgacaat cacatccgca gcgctacggg
    10921 caataagcag ttcgcgcggg ttagtggtca tacgggtcgc gccagcgccg atttttgcag
    10981 catcgccaac gcggtcaact ttgacgatca aatcaacctg atcttgctca atgcttgccg
    11041 gattatgcgg ataaggcagc agttcttcgg taagcatcac aacctgtttt gcgttgtcgg
    11101 catcaaccat tgcatagccg agggagccgc agcaggcttt accggtgtaa ccgttggcat
    11161 taccgaattc atcacaggac gggacgccga ggaaagccac gtcgatattc agttcgccgc
    11221 tctgtaccag atgcacacgt ccgccgtgag agtggatttg caccggttcc gcaagcagac
    11281 cacgggaaat ttcttctgcc agtggaccgc gcaggccgga ggtataaatg cgggtaacca
    11341 cgccctggcg aatgtgttct accagtggcg catggcaatc actcagggag ctggacgcca
    11401 gggtcaggtt tttaaagccc atcttagcga tgacgtccat caccatattg acggtcaggt
    11461 caccgccacg gaaagcgtga tggaaggaaa ccgtcatgcc gtcctgtaaa ccagagcgac
    11521 gaatcgcttc ttcctggttg gcgcacagtt tgcgatcgcg cgctttttca gcctggtagg
    11581 tttgctttgg tgagttctgg aaagcggcaa gatcgcattc agcgcgacga ttccaggccg
    11641 ctacccgttc ttgtcgttga gattgttcaa ttttctgcgt cattttgatt gccttattct
    11701 tcgcggatgc cggaaagttc tgcacgggag agcaccagac gggcacgatc gataaccgga
    11761 ccgtccacca tcttgccgtt cagggaaacc acgccgaggc cttcgcgagc ggcggcttca
    11821 gcggcttcta cgacgcggcg ggcgtgatcc acttctttct gggtcggtgc gtagaggttg
    11881 tgcaacagat caatctgacg cgggttgatc agcgatttgc cgtcaaagcc cagctgtttg
    11941 atgtgggcgg cttcttgcag gaatccggct tcgttgttag cgtcggaata gacggtatcg
    12001 aacgcctgaa tacccgcaga gcgcgcggcc tgcaaaatgg aacagcgtgc gaacagcagt
    12061 tcagttcctt ccggggagcg ttctgtacgc aggttgcgca catagtcttc tgcaccgagg
    12121 gcgataccga tcaaacgctc ggaagcgtga gcgatttcca ctgcgcgggt aatccccagc
    12181 ggagattcaa tcgccgccag caggccggtg ctgccgggtt cacgaccaca ggctttttcg
    12241 atacgcagga tctctttttc gatatccaga acatcctgag cggtatcggt tttcggcaga
    12301 cgcacaacgt ccgcaccacc gcgaacgacg gcttccaggt cgttgacacc ccattcggaa
    12361 tccagcgcgt tgacacgcac aatggtttca atatcgcgat acagcggatg ttgcagcgcg
    12421 tggtaaacca tgcggcgggc ggtgtctttt tcacgcaatg ctacggagtc ttcgaggtca
    12481 aacatcaggg catcagccgg gtagatgaag gagttgctga ccatcgcggc attggcacca
    12541 ggcacaaaca acatgctgcg gcgggtgcga gttttacgtt gttgcagcga agcggaaatc
    12601 attggcaatc ctcccatggc agagccggga taccgctggc gcgtgccagc agggcttcca
    12661 gtcgtgcacg taaaatgcag tccagtgcgc ctttgtcatc gacattcagc tgtacgccgc
    12721 gcacgttgta gcgggcgaga acgtccagaa tggtggtgcg aattgcatcg ccaaactgtt
    12781 tctcaacgct gctattgatt tgcaggtcga tatcctgcgt atcgagtggg gcgatgcgta
    12841 tcatcacatc cccagactca agggtgcctg caacggcggg ctggtttatt ttcatttttc
    12901 acctgtttct catgcggggg tcttttgacg agctgccgcg tcctggcggg agtgctcaag
    12961 caggttctgc aaataatgca gcgtgactgc agggaccagc ggcgcgatag ccgtgagatc
    13021 gtttttcgcc agcagttgac gtacccggga agcggatatc ggcatctcct ggtaacgcag
    13081 ccgctcaatt tcaaccagtt cgatgggcgg tgcggagata gtcggcgttt ccagccagta
    13141 gcgcatatcc tggttgtact gggcggtaac gcgacaaaag ggttcagtac cgacaaagcg
    13201 gtgagttaca cccagcgcgg gagcgaggta ctgacggaaa atcttcagat caatttcggt
    13261 gtaacaatgg ttaatgacgc tctgttcttt aatgaagtag caagggaacg tagcgcggga
    13321 gatgatgtat tcggagccgc gatgcacagt caggcgtgga atatcggcgg tgccttttaa
    13381 caccagatcc aaccggtctt cataggggaa gcgtgaagaa tcttctttga ctaaaaacag
    13441 atgcaaccag tcgcactgtg ccgcagcttg ttgaatcaga taacggtgac cattcgtgaa
    13501 gggattggcg ttcatcacaa tgcagccaat cttgttccct ggatgacgaa attttttcag
    13561 cgattcggca tagcgtttca gccgcgtggc gctgttttcc atcagcacca tcacgccggg
    13621 tacgctggtc agcgtggaaa aaccgcactg gcggaacagc gcctcgtatt cggttttggt
    13681 ataaataaac agatgcgtgc tgtgccgctc ataggcgagg tttatcaatt cagtggctaa
    13741 tgtcagcgcc agtccttcac cgcgaacgga ttcactgata gcaacgcatt taataatatt
    13801 tccggca
//
LOCUS       DAZPJR010000106        13794 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPJR010000106 DAZPJR010000000
VERSION     DAZPJR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.107"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..147)
                     /locus_tag="ACHVB7_002887"
     CDS             complement(<1..147)
                     /locus_tag="ACHVB7_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR1914817.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="ACHVB7_002888"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="ACHVB7_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="ACHVB7_002889"
                     /pseudo
     CDS             476..1021
                     /locus_tag="ACHVB7_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="ACHVB7_002890"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="ACHVB7_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1914818.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="ACHVB7_002891"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="ACHVB7_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR1914819.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="ACHVB7_002892"
     CDS             3028..3201
                     /locus_tag="ACHVB7_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914820.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            3227..3295
                     /gene="yncP"
                     /locus_tag="ACHVB7_002893"
     CDS             3227..3295
                     /gene="yncP"
                     /locus_tag="ACHVB7_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR1914821.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="ACHVB7_002894"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="ACHVB7_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR1914822.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="ACHVB7_002895"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="ACHVB7_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR1914823.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="ACHVB7_002896"
     CDS             complement(5499..6422)
                     /locus_tag="ACHVB7_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1914824.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="ACHVB7_002897"
     CDS             6639..7982
                     /locus_tag="ACHVB7_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1914825.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="ACHVB7_002898"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="ACHVB7_002898"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR1914826.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="ACHVB7_002899"
     CDS             10002..10226
                     /locus_tag="ACHVB7_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR1914827.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="ACHVB7_002900"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="ACHVB7_002900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR1914828.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /gene="ydcK"
                     /locus_tag="ACHVB7_002901"
     CDS             complement(10820..11800)
                     /gene="ydcK"
                     /locus_tag="ACHVB7_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR1914829.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="ACHVB7_002902"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="ACHVB7_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR1914830.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="ACHVB7_002903"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="ACHVB7_002903"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR1914831.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3197 c         3483 g         3574 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gcgcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catc
//
LOCUS       DAZPJR010000107        13760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPJR010000107 DAZPJR010000000
VERSION     DAZPJR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.108"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..80)
                     /locus_tag="ACHVB7_002904"
     CDS             complement(<1..80)
                     /locus_tag="ACHVB7_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914832.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGT"
     gene            255..2021
                     /gene="adeD"
                     /locus_tag="ACHVB7_002905"
     CDS             255..2021
                     /gene="adeD"
                     /locus_tag="ACHVB7_002905"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR1914833.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="ACHVB7_002906"
     CDS             complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="ACHVB7_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR1914834.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="ACHVB7_002907"
     CDS             complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="ACHVB7_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR1914835.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="ACHVB7_002908"
     CDS             complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="ACHVB7_002908"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR1914836.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="ACHVB7_002909"
     CDS             complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="ACHVB7_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR1914837.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="ACHVB7_002910"
     CDS             complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="ACHVB7_002910"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1914838.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="ACHVB7_002911"
     CDS             complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="ACHVB7_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR1914839.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="ACHVB7_002912"
     CDS             complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="ACHVB7_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR1914840.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9842..9931
                     /gene="tisB"
                     /locus_tag="ACHVB7_002913"
     CDS             9842..9931
                     /gene="tisB"
                     /locus_tag="ACHVB7_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR1914841.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(10055..10129)
                     /gene="ysdE"
                     /locus_tag="ACHVB7_002914"
     CDS             complement(10055..10129)
                     /gene="ysdE"
                     /locus_tag="ACHVB7_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR1914842.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10211..11395
                     /gene="emrD"
                     /locus_tag="ACHVB7_002915"
     CDS             10211..11395
                     /gene="emrD"
                     /locus_tag="ACHVB7_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR1914843.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11403..11900)
                     /locus_tag="ACHVB7_002916"
     CDS             complement(11403..11900)
                     /locus_tag="ACHVB7_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR1914844.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11897..12259)
                     /locus_tag="ACHVB7_002917"
     CDS             complement(11897..12259)
                     /locus_tag="ACHVB7_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR1914845.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12249..12596)
                     /locus_tag="ACHVB7_002918"
     CDS             complement(12249..12596)
                     /locus_tag="ACHVB7_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR1914846.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12705..13154
                     /gene="yidI"
                     /locus_tag="ACHVB7_002919"
     CDS             12705..13154
                     /gene="yidI"
                     /locus_tag="ACHVB7_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR1914847.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13201..13359)
                     /locus_tag="ACHVB7_002920"
                     /pseudo
     CDS             complement(13201..13359)
                     /locus_tag="ACHVB7_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13403..>13760)
                     /locus_tag="ACHVB7_002921"
     CDS             complement(13403..>13760)
                     /locus_tag="ACHVB7_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1914848.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3267 a         3816 c         3571 g         3106 t
ORIGIN      
        1 gtcccatgct gaggtagttt aaataatcgc gaaagcgtcc ctgattcatt actcacgtaa
       61 tcggtattgt cattattcat ttttttgtcc attggaggag atttaatccc ttgcttttgt
      121 aaaacaaatg acaacaacgg ttcagtgata attatcacat ttcaattgca cattaatgga
      181 tattctttaa taatctcgcg acgtttcttt atgataaata ataatcaaat tgataaaatc
      241 aaaatgagaa aaatatgaat aattctatta accataaatt tcatcacatt agccgggctg
      301 aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata
      361 atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag
      421 gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg cagcggattg
      481 atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca
      541 gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg acgaccgtta
      601 tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc
      661 gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc gtacccgccc
      721 tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc gcctggcggg
      781 accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc
      841 agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac ggtcactgcc
      901 cgggtttggg gggtaaagaa cttaacgcct atattactgc gggtattgaa aactgccacg
      961 aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg ttgatgatcc
     1021 gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac gaatttaaca
     1081 gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaaggac
     1141 acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat
     1201 atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg
     1261 cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc
     1321 aggtactggt gaaaggcgag ccgattgatg cgcaaacctt acaggcggaa gagtcggcga
     1381 gactggcaca atccgctccg ccatatggca acaccattgc ccgccagcca gtttccgcca
     1441 gcgactttgc cctgcaattt acgcccggaa aacgctatcg ggtcattgac gtcatccata
     1501 acgaattgat tacgcactcc cactccagcg tctacagcga aaatggtttt gatcgcgatg
     1561 atgtgtgctt tattgccgta cttgagcgtt acgggcaacg gctggctccg gcttgtggtt
     1621 tgcttggcgg ctttggactg aatgaaggtg cgctggctgc gacggtcagc catgacagcc
     1681 ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga
     1741 ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca ggtccaaagt catctgccgt
     1801 tgcccattgc cggcctgatg agcaccgaca cggcgcagtc gctggcggaa caaattgacg
     1861 ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg
     1921 cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg ctatttgatg
     1981 tcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaagc ccggcgtcat
     2041 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
     2101 ggcggatttt gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg
     2161 cggcgatatc aagtgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
     2221 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
     2281 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
     2341 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
     2401 agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
     2461 cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc agagagccag ccccacagca
     2521 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
     2581 agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca atacgtacca
     2641 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
     2701 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
     2761 tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg gagtcgctgc
     2821 cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag ataaacatgc
     2881 cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacgcct gctgcgcctg
     2941 caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt ttacgacgcg
     3001 gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
     3061 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
     3121 tgaagcccag catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt
     3181 ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg atggagaaac
     3241 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct
     3301 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
     3361 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
     3421 gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc
     3481 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca
     3541 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
     3601 ttcgcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
     3661 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg
     3721 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
     3781 tgccgcctct ttgtgggaac actctgccgc cgccataccg attaacatct gtgggccaaa
     3841 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
     3901 cagccacagg gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
     3961 tcggttgccg ttaaacaatt tgtccgagcc ccaacccgct accagcgcac cgataaatcc
     4021 acccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc
     4081 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
     4141 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt
     4201 acgcgtcagc cctgcccctt cttgttgttg agctatctcc agcgcgtcgt gtcgccattc
     4261 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat
     4321 tcccacgacg atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgtag
     4381 tgcagccgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
     4441 taatgcccac caaccaccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
     4501 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagtacgg caaacgccca
     4561 tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcgataa gccctatccc
     4621 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
     4681 ataggtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
     4741 agcaaggatt tctggtacgg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
     4801 acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc
     4861 atatttatca gccattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa
     4921 accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
     4981 gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
     5041 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc
     5101 atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt
     5161 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc
     5221 atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat
     5281 cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc
     5341 caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gttgcccgct
     5401 ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
     5461 agcagtgatg gtctggccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct
     5521 ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag
     5581 cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact
     5641 ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat
     5701 cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc
     5761 cagcgcgata atcggcagca ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa
     5821 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc
     5881 gcgccagttg attggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc
     5941 gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag
     6001 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
     6061 cgccgccgtt aacgccgccc cctgtaacag caaggtgcgc cagtcacgct gatggcgata
     6121 gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa
     6181 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cacccagcaa
     6241 tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
     6301 taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc acaggcaaaa
     6361 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
     6421 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacgcccagt
     6481 ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc cagttcggcg
     6541 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcacgtttg
     6601 gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
     6661 ccacccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggaa
     6721 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact gtcgtgaacg
     6781 gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag accggagata
     6841 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
     6901 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
     6961 tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
     7021 ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
     7081 ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga tcggactgat
     7141 tacgctgcac tttcacgaca tcttccagct tatcgatttg gcttatcatc tgctccagac
     7201 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
     7261 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
     7321 cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gtcatgagtt gtgttttgca
     7381 tggcttattc ccccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
     7441 tttcttcggc atcaatgcgc acatggatca gcgccgggct agggcgattg atgatttcct
     7501 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
     7561 cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg ccttgctcgt
     7621 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
     7681 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
     7741 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
     7801 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
     7861 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
     7921 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
     7981 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
     8041 ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg
     8101 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
     8161 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
     8221 gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta ctgcgcacgc
     8281 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg cccagcgcca
     8341 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgcgcaggcg
     8401 cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta atcattgtag
     8461 ctacgtcacg aatgctttct tcgctaaagg cgggtgcagc ggctttttct gccaccgcgg
     8521 gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct atccacaccg
     8581 ggccagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt
     8641 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcga
     8701 cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca
     8761 gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc
     8821 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa
     8881 agcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg
     8941 catcgtaaac aggcaggata gaaccgcccg gaatgcctgt cacaatctta atgccctgct
     9001 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc aatgttgtgc
     9061 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
     9121 gaaaaacccc gcccggtttg cgccgacggg gttttggaat cgtgtgttgt tccagtccct
     9181 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag
     9241 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
     9301 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
     9361 ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
     9421 gagcgaggtt tcgtcagtcg cccgcggctg gtaaccgcaa agcacactgt attatgtcaa
     9481 cacagaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
     9541 tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaatattgc tgtttattta
     9601 tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
     9661 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
     9721 cacactgtgt tatgtcaaca agcacaaagt ttctccttga gataccgcgt gcacaacagc
     9781 tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg
     9841 tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact
     9901 gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc
     9961 gggtaaccgc tcccctttta tatttagcgt gcgggttggt gtcggatgcg atgctgacgt
    10021 atcttatccg ccctaccatc tctcccggca acatttattg ccgcttttgt ttacatattc
    10081 tgccgctaaa caactcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
    10141 attcctctcc cgcatttatg ggaatgcgta gtgacttact ctaattattt ttataaaagc
    10201 atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg tattactcgt
    10261 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
    10321 tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
    10381 tgtctcacag ctgttttatg gcccgatttc cgaccgtgtg ggtcgccgac cggtgatcct
    10441 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct ccagtttgac
    10501 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
    10561 gcgtactttg ccgcgagatt tatatgaacg gacacagttg cgccatgcta acagcctgtt
    10621 aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc tgctggatac
    10681 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg gtgtgacctt
    10741 cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct
    10801 taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc tgatgctgat
    10861 tggcggtctg gccgggattg ccgcctttga agcctgctcc ggcgtgctga tgggcgcggt
    10921 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cagcagcgtt
    10981 ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tccacgttaa tgtggcagtc
    11041 ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
    11101 gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
    11161 tcctctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca cggctggcgc
    11221 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
    11281 gccgcaaacc ggtcagggca gcctagggtt gttgatgacc ttaatgggat tgttgatcgt
    11341 gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcccg tttaagcgca
    11401 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
    11461 atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
    11521 ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
    11581 tcacagcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc
    11641 tcaatcgaga tacgactgat atctgcccgc acccaacact cgaacagggc aattaaaaat
    11701 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
    11761 agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg
    11821 gcaggttgca gagcatcctt gtactctaca accagtttat gcctgtcctc ttcagcatca
    11881 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
    11941 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
    12001 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
    12061 agtgccagag gatcagcgcc acgatggcga gaatgccaat cgaaatccag aataacatgc
    12121 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
    12181 tacgaaacca cgccagtgat gtgcgctccg gctgcagtcc tgggtcggca atgcgtctgg
    12241 ctttgcggct atccggcata caacaccagt cccataacga tcaccgccac gaccatcaat
    12301 attaagctaa tgattaataa gctgttggtg tagggcaggt cctctttcag acgcatcgct
    12361 ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga aaacagacac
    12421 aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc aagttgatca
    12481 agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag aaaagtacgc
    12541 tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt
    12601 gctcctttgc aatttcacta actctctacc tttagcggtt ttttttaaga atttcgtccg
    12661 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagcgc
    12721 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12781 tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc atcgtggcgg
    12841 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12901 ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc ggtatcgcac
    12961 tggccattgc tgcgctactt tgcgcatttg ttggcggtat gcgtaaggaa aatcgctggg
    13021 gcattcgcgg cgcgctggtt tttggcggcg gaacgctggc tttccatacc ttgctttttg
    13081 gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc ctgaccggcg
    13141 gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    13201 tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaggc cggtgtcata
    13261 gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc ccatccagcg
    13321 cggccgtgca tctttacgcc acggacgcag gctccattgt tgacatcctc cacgccctga
    13381 aggacgtgga cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13441 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13501 aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13561 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacacg caacgctggc
    13621 ttgtgtacgc tggcggaaca gcaagcacct gaccaccacg ccagagctgc ttgtacgcaa
    13681 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
    13741 cccggacatt gcgccccggc
//
LOCUS       DAZPJR010000108        13731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPJR010000108 DAZPJR010000000
VERSION     DAZPJR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.109"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..114)
                     /gene="yojO"
                     /locus_tag="ACHVB7_002922"
     CDS             complement(<1..114)
                     /gene="yojO"
                     /locus_tag="ACHVB7_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR1914849.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIA"
     gene            223..717
                     /gene="napF"
                     /locus_tag="ACHVB7_002923"
     CDS             223..717
                     /gene="napF"
                     /locus_tag="ACHVB7_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR1914850.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            707..970
                     /gene="napD"
                     /locus_tag="ACHVB7_002924"
     CDS             707..970
                     /gene="napD"
                     /locus_tag="ACHVB7_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR1914851.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            967..3453
                     /gene="napA"
                     /locus_tag="ACHVB7_002925"
     CDS             967..3453
                     /gene="napA"
                     /locus_tag="ACHVB7_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR1914852.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3460..4155
                     /gene="napG"
                     /locus_tag="ACHVB7_002926"
     CDS             3460..4155
                     /gene="napG"
                     /locus_tag="ACHVB7_002926"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR1914853.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4142..5005
                     /gene="napH"
                     /locus_tag="ACHVB7_002927"
     CDS             4142..5005
                     /gene="napH"
                     /locus_tag="ACHVB7_002927"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR1914854.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5002..5451
                     /gene="napB"
                     /locus_tag="ACHVB7_002928"
     CDS             5002..5451
                     /gene="napB"
                     /locus_tag="ACHVB7_002928"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR1914855.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5461..6063
                     /gene="napC"
                     /locus_tag="ACHVB7_002929"
     CDS             5461..6063
                     /gene="napC"
                     /locus_tag="ACHVB7_002929"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR1914856.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6076..6699
                     /gene="ccmA"
                     /locus_tag="ACHVB7_002930"
     CDS             6076..6699
                     /gene="ccmA"
                     /locus_tag="ACHVB7_002930"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR1914857.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6696..7358
                     /gene="ccmB"
                     /locus_tag="ACHVB7_002931"
     CDS             6696..7358
                     /gene="ccmB"
                     /locus_tag="ACHVB7_002931"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR1914858.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7400..8137
                     /gene="ccmC"
                     /locus_tag="ACHVB7_002932"
     CDS             7400..8137
                     /gene="ccmC"
                     /locus_tag="ACHVB7_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR1914859.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8134..8343
                     /gene="ccmD"
                     /locus_tag="ACHVB7_002933"
     CDS             8134..8343
                     /gene="ccmD"
                     /locus_tag="ACHVB7_002933"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR1914860.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8340..8819
                     /gene="ccmE"
                     /locus_tag="ACHVB7_002934"
     CDS             8340..8819
                     /gene="ccmE"
                     /locus_tag="ACHVB7_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR1914861.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8816..10759
                     /gene="ccmF"
                     /locus_tag="ACHVB7_002935"
     CDS             8816..10759
                     /gene="ccmF"
                     /locus_tag="ACHVB7_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR1914862.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10756..11313
                     /gene="dsbE"
                     /locus_tag="ACHVB7_002936"
     CDS             10756..11313
                     /gene="dsbE"
                     /locus_tag="ACHVB7_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR1914863.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11310..12362
                     /gene="ccmH"
                     /locus_tag="ACHVB7_002937"
     CDS             11310..12362
                     /gene="ccmH"
                     /locus_tag="ACHVB7_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR1914864.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12397..13044)
                     /gene="narP"
                     /locus_tag="ACHVB7_002938"
     CDS             complement(12397..13044)
                     /gene="narP"
                     /locus_tag="ACHVB7_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR1914865.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13364..>13731
                     /locus_tag="ACHVB7_002939"
     CDS             13364..>13731
                     /locus_tag="ACHVB7_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1914866.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         2935 a         3458 c         4033 g         3305 t
ORIGIN      
        1 cgctatataa atatgtttat aaccatttga aatgtgagca aaagcccgtt tttgccacgc
       61 ttcccgccac tcttttgatc ctgctacagg ttttaccccg atcggggtat gcatctttga
      121 cacatccttt aatatcttag cggctataaa aatggcttat taattatgcg gtttatttgg
      181 tcgctctcaa ttttcagagc gcgttaatga tggaaggtca atgtgaagat tgatgcatcc
      241 cgtcggggca tactcactgg tcgctggcgc aaagccagta acggtatccg tccgccctgg
      301 tccggtgatg aatctcattt tctgacccat tgcacccgtt gcgacgcctg tattaatgct
      361 tgtgaaaaca acattctgca acgcggcgcg ggcggctatc cgagcgttaa tttcaaaaat
      421 aacgagtgca gcttctgtta cgcctgcgcg caggcttgcc ccgaatcgct attttctccg
      481 cgccacacca gggcctggga tttgcagttc accattgggg acgcgtgcct ggcgtatcag
      541 tcagttgaat gccgccgctg tcaggatagc tgtgagccaa tggcaattat ctttcgcccg
      601 acgctctccg ggatctacca gccgcaactt aatagccaac tttgcaacgg atgtggagca
      661 tgtgcagcca gctgtccggt atcagccata actgcggagt atctccatgc acactaactg
      721 gcaagtttgc agcctggtcg tgcaggccaa aagcgaacga atttcagaca tcagcaccca
      781 actgaacgcc tttcccggct gtgaagttgc tgtcagcgac gcgccgagcg gtcagttgat
      841 tgtggtggtg gaagcagaag acagcgaaac gctgatccaa accattgagt cagtacgcaa
      901 cgtagagggc gtgctggcgg tgtcgctggt ttatcaccag caggaagagc aaggtgagga
      961 aacaccatga aactcagtcg tcgtagcttt atgaaagcta acgccgttgc ggccgctgcg
     1021 gcggctgccg gtctcagcgt gccgggcgtt gcccgcgccg ttgttggtca gcaggaagcc
     1081 attaaatggg ataaagcgcc gtgccgtttc tgcggtactg gttgcggcgt tctggtcgga
     1141 acgcagcagg ggcgtgtggt ggcctgtcag ggcgacccgg acgcaccggt taaccgtggc
     1201 ctgaactgca ttaagggcta tttcctgccc aagatcatgt acggtaaaga ccgtttgacg
     1261 cagccgctgc tgcgtatgaa aaacggtaaa tatgacaaag aaggcgaatt taccccaatc
     1321 acctgggatc aggccttcga tgtgatggaa gagaaattca aaaccgccct gaaagaaaaa
     1381 gggccggaat cgatcggtat gttcggttct ggtcagtgga ctatctggga aggttatgcc
     1441 gcgtccaagc tgtttaaagc gggcttccgt tcgaacaaca tcgacccgaa cgcgcgtcac
     1501 tgtatggcgt cggcagtagt tggctttatg cgtacctttg gtatggatga gccgatgggc
     1561 tgctatgacg acatcgagca ggctgacgcg tttgtgctgt ggggcgcaaa catggcggag
     1621 atgcacccga tcctctggtc gcgcatcact aaccgtcgtc tctctaatca gaacgtcacc
     1681 gtggcggtgc tttctaccta ccagcatcgt agcttcgagc tggcggataa cggcatcatc
     1741 tttacgccac agtctgacct ggtgatcctg aactacatcg ccaactatat cattcaaaac
     1801 aatgcgataa atcaggactt cttcagcaaa cacgttaacc tgcgcaaagg ggcgacggac
     1861 atcggctacg gtttacgtcc gacccatccg ctggaaaaag cagcgaagaa tccaggttct
     1921 gacgcctccg aaccgatgag ctttgaagat tacaaagcct tcgttgccga gtatacgctg
     1981 gaaaaaaccg ctgaaatgac cggcgtgccg aaagaccagt tagaacaact ggcgcagctg
     2041 tatgccgatc cgaacaagaa agtcatctcc tactggacga tgggcttcaa ccagcatact
     2101 cgtggcgtgt gggctaacaa cctggtctac aacctgcacc tgctgaccgg caaaatttcc
     2161 cagccgggtt gcggtccgtt ctccctgacc gggcaacctt ctgcgtgtgg tactgcgcgt
     2221 gaagtgggca cctttgctca ccgtctgcct gcggacatgg tggtgaccaa cgagaaacac
     2281 cgtgatatct gcgagaagaa gtggaatatc ccgagcggca ccattccggc gaaaatcggt
     2341 ctgcacgcgg tggcacaaga ccgtgcgctg aaagacggca aactgaatgt ttactggacc
     2401 atgtgtacca acaacatgca ggccgggccg aacatcaatg aagagcgtat gccgggctgg
     2461 cgcgatccgc gcaacttcat catcgtctcc gatccgtatc cgacagtcag tgcgctggcc
     2521 gccgacttga tcctgccgac cgcaatgtgg gtagagaaag agggtgctta cggtaacgcc
     2581 gaacgccgta ctcagttctg gcgtcagcag gtacaggcac cgggcgaagc gaaatcggat
     2641 ctctggcagt tagtccagtt ctcccgccgc ttcaaaactg aagaagtgtg gccggaagag
     2701 ctgctagcga agaaaccgga actgcgtggc aaaacgctgt acgaagtgct ttatgccaca
     2761 ccggaagtga gcaaattccc ggtatccgaa ctggcggaag atcagctgaa cgatgaatcc
     2821 cgcgagctgg gcttctatct gcaaaaaggg ctgttcgaag agtacgcatg gtttggtcgc
     2881 ggtcacggtc acgatctcgc gccgttcgat gactaccaca aagcgcgcgg tctgcgctgg
     2941 ccggtggtga acggtaaaga aacgcagtgg cgttacagcg aaggtaacga cccgtacgtg
     3001 aaagcgggcg aaggctataa gttctacggt aaaccggatg gtaaagcggt gatcttcgcg
     3061 ctgccgttcg aaccggcggc ggaagcaccg gatgaagagt acgacctgtg gctctctacc
     3121 ggacgcgttc tggagcactg gcacactggc agtatgactc gccgtgtgcc ggaactgcac
     3181 cgcgccttcc cggaagcggt cttgtttatt cacccgctgg atgcgaaagc gcgcgatctg
     3241 cgccgtggcg acaaagtgaa agtggtttct cgccgtggcg aagtgatctc gattgttgaa
     3301 acgcgcggtc gtaaccgtcc gccacagggc ctggtgtaca tgccgttctt cgacgccgca
     3361 cagctggtta acaaactaac gctggacgcg accgatccgc tctcgaaaga gacggacttc
     3421 aagaagtgcg cagtcaaact ggagaaggtg taagccatta tgtcccggtc agcgaaacct
     3481 caaaatggtc gccgccgctt tctgcgcgat gttgttcgca cagcaggcgg gctggctgcc
     3541 gtgggtgtgg cgctggggtt acaacagcaa accgcacgcg catctggcgt gcggttgcgc
     3601 ccgcccggag ccataaacga gaacgccttt gccagtgcct gtgtgcgttg cggtcagtgt
     3661 gttcaggctt gcccttacga caccttaaaa ctggcgacgc tggcttctgg cctgtcggcg
     3721 ggcacgccat attttgtcgc acgggatatt ccttgcgaaa tgtgtgagga cattccgtgc
     3781 gccaaagtgt gcccgagtgg tgcgctggat cgtgagatcg aatcgatcga cgacgcgcgg
     3841 atggggctgg cggtgctggt ggaccaggaa aactgtctca actttcaggg gctgcgctgc
     3901 gatgtttgtt atcgcgaatg cccgaaaatt gatgaggcca tcaccctgga gctggagcgc
     3961 aacacgcgta ccggtaagca cgcccgcttt ctgccgacgg ttcacagcga cgcctgtacc
     4021 ggctgcggta agtgcgaaaa agtgtgcgtg ctggaacaac cggcaatcaa ggtgttaccg
     4081 ctgtcactgg cgaaagggga gttaggtcac cattaccgct tcggctggct ggaggggaac
     4141 aatggcaaat cgtaaacgtg acgccgggcg cgaggcgctg gtgaaaaaag gctggtggcg
     4201 cagtcaccgt tggctggtgt tacgtcgcct ttgtcagttc ttcgtgctgg ggatgttttt
     4261 gagcggtccg tggtttggtg tgtggatctt acacggcaac tacagctgta gcctgttatt
     4321 cgacaccgtt ccgctgaccg atccgctgat gacgctgcaa agtcttgcca gtggtcatct
     4381 gcctgccacg gtggcgttga ccggtgcggt gataatcact gtgctctacg ccctggcagg
     4441 aaagcgatta ttttgcagct gggtctgccc gctgaacccg attaccgact tagcgaactg
     4501 gttacgcagg cgctttgacc tcaatcagtc tgcgacgatc cctcgccaca ttcgctacgt
     4561 gctgttggtg gtcattctgc tgggatcagc cttaaccggc acgctcatct gggaatggat
     4621 caaccccgtt tccctgatgg ggcgtagcct ggtaatgggc ttcggcagcg gcgcgctgct
     4681 tattctcgcg ctgtttttat ttgatctact ggtcgttgaa cacggctggt gcgggcacat
     4741 ttgtccggtt ggcgcgctgt atggcgtgct gggtagcaaa ggtgtgatta ccgttgcggc
     4801 cagcgatcgg cagaaatgta accgctgtat ggattgtttt catgtttgcc cggaaccgca
     4861 tgtgctacgt gccccggtgc tggatgaaca aagcccggtg caggtcacca gccgcgattg
     4921 catgacgtgc ggtcgctgcg tggatgtctg ttctgaggat gtatttacaa taactacacg
     4981 atggagttcg ggagcgaaat catgaaaagc catgacctga agaaagcgct gtgtcaatgg
     5041 acggcgatgc tggccctggt ggtaagcggc gcggtttggg cggctaatgg cgtcgatttt
     5101 agccagtcgc cggaagtatc cgggacgcag gaaggggcca ttcggatgcc aaaagagcag
     5161 gatcggatgc cgctgaacta tgtgaatcag ccgccaatga tcccgcatag cgttgaaggt
     5221 tatcaggtaa cgaccaatac caaccgctgc ttgcagtgcc acggtgtcga aagctatcgc
     5281 accactggcg cgccgcgtat cagtcctact cactttatgg acagcgacgg caaagtgggc
     5341 gcggaagtgg ctccgcgtcg ttatttctgt ctgcaatgtc acgtaccgca ggccgatacc
     5401 gcgccaatcg tggggaatac ctttacccca tcaaaaggtt acgggaaata agaggttatt
     5461 atgggaaatt ctgaccgtaa gcctggtctg attaagcgcc tgtggaaatg gtggcgtacc
     5521 cccagccgtc tggcgctggg aacgctgctg ttgatcggtt ttgtcggcgg catcgtcttc
     5581 tggggcggct ttaacaccgg aatggaaaaa gccaataccg aagagttctg cattagctgc
     5641 cacgaaatgc gtaacacggt gtatcaggaa tacatggatt ccgtgcacta caacaaccgt
     5701 agcggcgtcc gtgcgacctg tccggattgt cacgttccgc acgagtttgt gccgaagatg
     5761 atacgcaagc tcaaagcaag taaagagctg tatggtaaaa tttttggcgt tattgacacg
     5821 ccgcagaaat ttgaagctca tcgtctgaca atggcacaga atgagtggcg gcgcatgaag
     5881 gacaataact cgcaggagtg ccgtaactgt cacaacttcg agtatatgga tacaaccgcc
     5941 cagaaatcgg ttgccgcgaa gatgcatgac caggcggtga aagatgggca aacctgtatt
     6001 gattgccata aagggatagc gcacaagctg cccgatatgc gtgaagtcga gccaggtttt
     6061 taacagggtt attgcgtggg tatgcttgaa gccagagagt tactttgtga gcgggatgaa
     6121 cgaaccttat ttagtggctt gtcatttacg ctgaacgcag gagagtgggt acaaatcacc
     6181 ggtagcaacg gcgcggggaa gacaacgctt ctccgtttgc tgacggggtt gtctcgccct
     6241 gacgcaggcg aggttctctg gcaagggcag cccttgcatc aggtacgcga cagctaccat
     6301 caaaacctgt tatggatagg ccatcagccg gggatcaaaa cccggctgac ggcgttagaa
     6361 aatctgcact tttatcatcg cgatggcgat accgcacaat gtctggaagc cctggcgcag
     6421 gccgggcttg ccggattcga cgatattccg gtaaatcaac tttctgccgg gcaacaacgc
     6481 cgcgtcgctt tagcgcgtct gtggctgacc cgtgccacgt tatggatcct cgacgagcct
     6541 tttaccgcga ttgacgttaa cggcgtcgat cgtctgaccc agcgtatggc gcagcatacg
     6601 gagcaggggg ggattgtgat tctgactacc caccagccgc tcaacgttgc tgaaagtaaa
     6661 attcgccgca tttcactgac gcaaacgagg gccgcatgat gttctggcgc attttccgtc
     6721 ttgagctgcg tgtagcgttt cgccatagcg ccgaaatcgc caacccgctg tggttcttcc
     6781 tgattgtaat tacccttttt ccgctcagta tcggtccgga gccgcaactg ctggcgcgta
     6841 ttgcaccggg cattatctgg gttgctgcgc tgctttcatc cttgctggcg ctggaacgac
     6901 tgttccgtga cgatttgcag gacggcagtc ttgaacaatt gatgttgttg ccgttaccct
     6961 tgcccgccgt tgtgctggcg aaggtgatgg cgcactggat ggtaaccggt ctgccgttac
     7021 tcatcctttc gccactggta gcaatgctac tgggaatgga tgtttatggc tggcaagtga
     7081 tggcgctgac gctgctgctg ggaacgccta cgcttggctt tctcggtgca ccgggtgtgg
     7141 cgttgacagt gggacttaag cgcggtggtg tgctgctcag catactggtg ttaccgctga
     7201 ctatcccatt actcatcttt gccaccgccg cgatggacgc ggcttccatg catttgcccg
     7261 ttgacgggta tctggcaatt ttaggcgcgt tgctggcagg caccgcgaca ttaagtcctt
     7321 ttgcgacggc ggcagcgtta cgaatcagca ttcaataacg ctggattact gatttttgtt
     7381 cgagtctggt atcgaaacta tgtggaaaac actgcatcaa ctggcgatcc caccacggct
     7441 gtatcaaatc tgtggctggt ttataccgtg gctggcaatt gccagtgtgg tcgtgcttac
     7501 cgtcggctgg atctggggat tcggctttgc tccggctgat tatcagcagg gaaatagcta
     7561 ccgcattatc tacctgcatg tgcctgcggc gatctggtcg atgggcattt atgcatcaat
     7621 ggcagtggca gcgtttattg gccttgtctg gcagatgaaa atggccaacc tggcggtggc
     7681 ggcgatggcc cccattggtg ccgtgtttac ctttattgcc ctggttaccg gctctgcatg
     7741 gggaaaaccg atgtggggca cctggtgggt atgggatgca cgtctgactt ctgaactggt
     7801 gctgctgttt ttgtatgtgg gtgtgattgc cctgtggcac gccttcgacg accgccgtct
     7861 ggcgggccgt gcggcaggta tcctggtgct gattggcgtg gtgaatctgc cgattattca
     7921 ttactccgtg gagtggtgga acaccctgca tcagggatca acgcggatgc agcaaagtat
     7981 cgatccggcg atgcgttcgc cgctgcgctg gtcgattttt ggcttcctgc tcctgtctgc
     8041 cacgctgacg ctgatgcgga tgcgtaattt gattttgctg atggaaaaac gccgtccgtg
     8101 ggtgagtgaa ctgatactga aaagaggccg taaatgaccc ctgcatttgc ttcctggaat
     8161 gaatttttcg caatgggcgg ttacgccttt tttgtctggc tggcggtggt gatgaccgtt
     8221 attccgctgg tggttttggt cgtgcactcg gtgatgcaac atcgcgcaat tctgcgtggt
     8281 gtggcgcaac agcgggcgcg tgaggcgcgt ttacgtgctg cgcaacagca ggaggctgca
     8341 tgaatattcg ccgtaaaaac cgcttgtgga ttgcctgtgc cgtgttggca gggctggcgc
     8401 tgactatcgg tctggtgcta tatgcgctgc gctcgaatat cgatctcttt tatacgccgg
     8461 gggaaattct ctacggcaag cgtgaaactc agcaaatgcc ggaagtcggt cagcgtttgc
     8521 gcgttggcgg gatggtgatg ctgggtagtg tgcagcgcga tcccaattcg ctgaaagtga
     8581 ccttcactat ttacgatgct gaaggctcag tggatgtctc ttacgaaggc attttgccgg
     8641 atctgttccg tgaagggcag ggcgttgtgg tgcagggcga gctggaaaaa ggcaatcata
     8701 tcctcgcgaa agaagtgctg gcgaaacacg acgaaaacta cacgccgcca gaagttgaga
     8761 aagcgatgga agctaaccac cgtcgcccgg cgagtgttta taaggaccca gcatcatgat
     8821 gccggaaatt ggaaacgggc tgctgtgtct ggcgttaggg attgcgctgc tgctgtccgt
     8881 gtatccgcta tggggcgtgg cgcgcggaga tgcgcgcatg atcgcgtctt cccgcttgtt
     8941 tgcctggctg ctgtttatgt ctgtggctgg cgcatttctg gtgctggtca atgctttcgt
     9001 ggtcaacgac tttaccgtca cctatgttgc cagctactcc aatacccagc ttccggtgtg
     9061 gtatcgcgtg gcggctacct ggggcgcgca tgaaggctcg ctactgctgt gggtgctgct
     9121 gatgagcggc tggacctttg cggtggcgat ttttagtcag cgtattccgc tggatattgt
     9181 ggcccgcgta ctggcgataa tggggatggt cagcgtcggc tttttgctgt tcattctctt
     9241 tacctctaac ccattctcac gcacgctgcc gaacttcccg attgaagggc gcgatcttaa
     9301 cccgctgtta caggacccgg ggctgatctt ccatccgcct ctgctttata tggggtacgt
     9361 gggtttctcg gtggcgtttg cttttgccat tgcttctttg ctgagcgggc gtctggacag
     9421 cacttatgcg cgttttactc gcccgtggac gctggcggcg tggattttcc tgacgctcgg
     9481 cattgtgctc ggttccgcat gggcctatta cgaactcggc tgggggggct ggtggttctg
     9541 ggatccggta gaaaacgcct cgtttatgcc gtggctggtg gggactgcgc tgatgcactc
     9601 actggcggtc actgaacaac gcgccagctt caaagcgtgg acattactgc tggcaatcag
     9661 tgccttctcg ttgtgtctgt tggggacttt cctcgtgcgt tccggcgtgc tggtatcggt
     9721 acacgcgttt gcgtctgatc cggcgcgcgg tatgtttatc ctcgccttta tggtgctggt
     9781 gattggaggt tcgctgctgc tgtttgccgc gcgtggacac aaagttcgct cacgcgtaaa
     9841 caatgcgctg tggtcgcggg aatctctgct gttagcgaac aacgttttgc tggtcgccgc
     9901 gatgctggtg gtgttgctgg ggacgctgct gccgctggtg cacaagcaac tgggactggg
     9961 cagtatttcg attggcgaac cgttcttcaa caccatgttt acctggctga tggtgccgtt
    10021 tgcgctactg ctgggtgtcg gtcctctggt gcgctggggg cgcgatcgcc cacgtaaaat
    10081 ccgcaattta ttgattatcg ccttcatctc aacgctggtg ctgtctctgc tgttgccgtg
    10141 gctgtttgag agcaaagttg tggcgatgac ggtgctcggc ctggcaatgg cctgctggat
    10201 tgcggtgctg gcaattgcgg aagctgcgct gcgtatttca cgcggcacga aaaccacctt
    10261 cagttattgg gggatggtgg ctgctcacct ggggctggca gtgacaattg ttggcattgc
    10321 ttttagccag aactatagcg ttgagcgtga tgtgcgcatg aagtccggcg atagcatcga
    10381 tattcatgaa tatcgcttca ccttccgtga tgtcaaagag gtgactggcc cgaactggcg
    10441 tggcggtgtg gcgactatcg gcgtaacgcg agagggtaaa ccggaaacag tgctgtatgc
    10501 ggaaaaacgt tattacaaca ctgccgggtc gatgatgacc gaagcggcga ttgacggtgg
    10561 catcacgcgt gacctgtacg ccgcgctcgg tgaagagctg gaaaacggcg cgtgggctgt
    10621 gcgtctttac tacaaaccat ttgttcgctg gatttgggcg ggcgggctga tgatggcgtt
    10681 gggcggactg ctgtgtctgt ttgatcctcg ctatcgtaag cgcgtgagtc cgcaaaaaac
    10741 tgcgccggag gctgtatgaa gcgcaaagta ttgttaattc cgttgattat cttcctggcg
    10801 attgccgcgg cgctgctgtg gcagctggcg cgtaatgccg aaggggatga tccgaccaat
    10861 ctggaatcgg cgctcattgg caagccagtg ccgaagtttc gcctcgaatc actggacaat
    10921 ccggggcagt tttatcaggc ggatgtgctg actcagggca aaccagtgct gcttaacgtc
    10981 tgggcgacct ggtgtccgac ctgccgtgcg gaacatcaat atctgaatca gctttctgcg
    11041 cagggcatcc gcgtagtcgg catgaactat aaagacgatc gccagaaggc aatcagctgg
    11101 ctgaaagagc tgggcaatcc ttacgcgcta agcctgtttg atggcgatgg catgttaggg
    11161 ctggatctcg gtgtctatgg cgcgccagaa acgttcctta ttgacggcaa cggcatcatt
    11221 cgctatcgcc atgcgggcga tctcaatcct cgcgtctggg aagaagagat caagccgctg
    11281 tgggagaaat acagtaagga ggccgcacaa tgaggttttt attgggcatg ctgatgctga
    11341 tgatctccgg ctcagcgctg gcgaccatag acgtgttgca gtttaaagat gaagcacagg
    11401 aacaacagtt ccgtcagctc actgaagaac tgcgctgccc gaaatgccag aacaacagca
    11461 ttgccgattc caactcgatg attgccaccg acctgcgcca gaaagtgtat gaactgatgc
    11521 aggaaggtaa aagtaaaaaa gagattgtcg attatatggt ggcgcgttac ggcaacttcg
    11581 tcacttacga tccgccgtta acgccgctga ccgtgctgct gtgggtgctg ccagtagtag
    11641 ctattggcat tggcggttgg gtcatatacg cccgctcgcg gcgtcgggta cgcgtggtgc
    11701 cggaagcgtt tcctgaacaa agcgtgccgg aaggtaagcg tgccggatat gtcgcttatt
    11761 tgccgggcat tgtggtggcg ttaattgtgg ctggcgtcag ctactaccag accggaaatt
    11821 atcaacaggt gaaaatctgg cagcaggcca cggcacaggc tccggcgttg ctggacaggg
    11881 cgctggatcc gaaagctgat ccgctcaacg aagaagagat gtcgcgcctt gcgctgggga
    11941 tgcgtactca actgcaaaaa aatccgggag atatagacgg ctggattatg ttgggccgcg
    12001 ttggcatggc gctgggtaac gccagtatcg ccaccgatgc atacgctact gcgtatcgcc
    12061 tcgatccgaa aaacagtgat gccgcactgg gttatgctga agcgttgaca cgttcatctg
    12121 atcccaacga caaccgcctc ggcggcgaac tgctacgtca gctggtgaga acggaccata
    12181 gcaatatccg tgtgctaagc atgtatgcgt ttaatgcctt tgagcagcag cgatttggcg
    12241 aagccgttgc cgcgtgggag atgatgttga aactcttacc tgccaacgat actcgccgtg
    12301 cggtgattga acgtagtatc gcgcaggcga tgcaacattt gtcgccgcag gagagtaaat
    12361 aaaaagaggc ccgatggtga tgccatcggg ctatttttat tgtgccccgc gttgttgcag
    12421 gaacagaatg gtggccgcca cgcgtgagcg gacattgagt ttacgcagca gatttcgaat
    12481 atgaactttt actgtctgct cggaaatatt caacacagag gcaatctgtt tatttgacag
    12541 cccctgtgcc agctcgtgca gaacatccag ctcgcgctcc gtcagcacgc tgaagggatc
    12601 ttcttccgcg ccaaacattt cacgttcacg taagtactga ttgacgcgtt cgctaaagac
    12661 tttgctgcct ttcgctccgg cacgaatcgc ttccagcaac acttccgggt cgctgtcttt
    12721 cagcagataa ccgtctgcgc ctgcgtctat cagcgcaaag acatcgctgg aggcatcgga
    12781 tacggtcagg ataataattt gcgcggtaac gccatccctg cgcaaggcat tgagggtatc
    12841 caggccactc atacctttca tattgagatc cagcaagatc acgtcgatat ccagtctatt
    12901 cgccagatcg atcgcgctcg cgccgtcgcc cgcttcggcg accacttcaa agccaggatc
    12961 aagctccagt aactgacgaa caccgcgtcg cataagtgga tgatcatcca caatcatcac
    13021 ctgaaaaggc gttgcttcag gcatagtagt ctcctgaggt tttattagaa tcattatgtt
    13081 tgagcgtagc gttggtaacg ctaccaggcg cgtgaatgtt accccatttt atgtatggac
    13141 atgtagtgaa aactacaacc ttgccgttcg ctttggcatc attttcatta taaagacgga
    13201 attttgcatc agcttctcat caagaaaaac aactaaaaaa tcaatcacaa tacttgtgca
    13261 tttctttaaa cagtttttaa aaactgactc cgggtatgga gctatgggta ttttctgtac
    13321 ccaatgcttt taacagcaat taatttcata ggatgaaagc tcaatgcatc aatctggttc
    13381 tgtttctctt tgtcgttccg caatatctgt tctggtggct acagcgttat attcacccat
    13441 agcattggca tcaacagttg agtatggtga gacagttgat ggtgttggcc tggaaaaaga
    13501 tatccagctg gtttatggga ccgccaataa tacgaaaatc aatcctggcg gagaacagca
    13561 tataaaagaa tttggtgtaa gtagtaatac tgaaattaac ggcgggtatc agtacattga
    13621 aatgaatggc actgcagaat actcagtatt aaatgatggt tatcaaattg ttcaaatggg
    13681 tggcgcggta aaccagacta cgctcaataa tggagtgcta caggtttatg g
//
LOCUS       DAZPJR010000109        13714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPJR010000109 DAZPJR010000000
VERSION     DAZPJR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.110"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(345..608)
                     /gene="rpsT"
                     /locus_tag="ACHVB7_002940"
     CDS             complement(345..608)
                     /gene="rpsT"
                     /locus_tag="ACHVB7_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR1914867.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            710..928
                     /locus_tag="ACHVB7_002941"
     CDS             710..928
                     /locus_tag="ACHVB7_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR1914868.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            936..1877
                     /gene="ribF"
                     /locus_tag="ACHVB7_002942"
     CDS             936..1877
                     /gene="ribF"
                     /locus_tag="ACHVB7_002942"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR1914869.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQKEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1920..4736
                     /gene="ileS"
                     /locus_tag="ACHVB7_002943"
     CDS             1920..4736
                     /gene="ileS"
                     /locus_tag="ACHVB7_002943"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR1914870.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4736..5230
                     /gene="lspA"
                     /locus_tag="ACHVB7_002944"
     CDS             4736..5230
                     /gene="lspA"
                     /locus_tag="ACHVB7_002944"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR1914871.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5355..5804
                     /gene="fkpB"
                     /locus_tag="ACHVB7_002945"
     CDS             5355..5804
                     /gene="fkpB"
                     /locus_tag="ACHVB7_002945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1914872.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5806..6756
                     /gene="ispH"
                     /locus_tag="ACHVB7_002946"
                     /gene_synonym="lytB"
     CDS             5806..6756
                     /gene="ispH"
                     /locus_tag="ACHVB7_002946"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR1914873.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6822..7736
                     /gene="rihC"
                     /locus_tag="ACHVB7_002947"
     CDS             6822..7736
                     /gene="rihC"
                     /locus_tag="ACHVB7_002947"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR1914874.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7903..8724
                     /gene="dapB"
                     /locus_tag="ACHVB7_002948"
     CDS             7903..8724
                     /gene="dapB"
                     /locus_tag="ACHVB7_002948"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR1914875.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            9180..10328
                     /gene="carA"
                     /locus_tag="ACHVB7_002949"
     CDS             9180..10328
                     /gene="carA"
                     /locus_tag="ACHVB7_002949"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR1914876.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            10346..13567
                     /gene="carB"
                     /locus_tag="ACHVB7_002950"
     CDS             10346..13567
                     /gene="carB"
                     /locus_tag="ACHVB7_002950"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR1914877.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3211 a         3426 c         3844 g         3233 t
ORIGIN      
        1 agatctaata aggagatatt tatgaatggt taaagtgtat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccacattct ttgtagggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga aagtgtaacg gcatgtctgc aaaagccagc atcttttgac
      241 tatctggcaa ccggtagtgt gttctctcac gcatcataaa agcagcaggc ataaaaaaaa
      301 cccgcttgcg cgggcttttt cacaaagctt cagcaaattg gcgattaagc cagtttgttg
      361 atctgtgcag tcaggttagc cttatgacgt gcagctttgt ttttgtggat cagaccttta
      421 gcagcctgac ggtccacgat cggttgcatt tcgttaaatg ctttctgtgc agcagctttg
      481 tcgccagctt cgatagctgc gtatactttc ttgatgaaag tacgcatcat agagcgacgg
      541 cttgcgttgt gcttacgagc cttttcagac tgaatggcgc gcttcttagc tgatttgata
      601 ttagccaagg tccaactccc aaatgtgttc tatatggaca attcaaaggc cgaggaatat
      661 gcccttttag ccttcttttg tcaatggatt tgtcaaataa gcgccgttaa tgtgccggca
      721 ctcgttacgt agtgatggcg caggattcta ccagcttgcg gggtgtgaat acagcttttc
      781 cgcgataaaa attgcagcag gcggtcactt tcttcccgtg atttgcgcca tggcaatgaa
      841 aagccacttc tttctgattt cggtactcaa tcgccggtta accttgaccg ctgtacaagg
      901 tatactcgga cgattttcac tgttttgagc cagacatgaa gctgatacgc ggcatacata
      961 atctcagcca ggccccgcaa gaagggtgtg tgctgactat tggtaatttc gacggcgtgc
     1021 atcgcggtca tcgcgcgctg ttacagggct tgcagaaaga agggcgcaag cgcaacttac
     1081 cggtgatggt gatgcttttt gaacctcaac cactggaact gtttgctacc gataaagccc
     1141 cggcaagact gacccggctg cgggaaaaac tgcgttacct tgcagagtgt ggcgttgatt
     1201 acgtgctgtg cgtgcgtttc gacaggcgtt tcgcggcgtt aaccgcgcaa aatttcatca
     1261 gcgatcttct ggtgaagcat ttgcgcgtaa aatttcttgc cgtaggggat gatttccgct
     1321 ttggcgctgg tcgtgaaggc gatttcttgt tattacagaa agctagcatg gaatacggct
     1381 tcgatatcac tagtacgcaa actttttgcg aaggtggcgt gcgtattagc agcaccgctg
     1441 tgcgtcaggc gctggcggat gacaatctgg ctctggcaga aagtttactg gggcacccgt
     1501 ttgccatctc cgggcgtgta gtccacggtg atgaattagg gcgcactata ggtttcccga
     1561 cggcgaatgt accgctacgc cgtcaggttt ccccggtgaa aggggtttat gcggtagaag
     1621 tgctgggcct cggtgaaaag ccgttacccg gcgtggcaaa catcggaaca cgcccaacgg
     1681 ttgccggtat tcgccagcag ctggaagtgc atttgttaga tgttgcaatg gacctttacg
     1741 gtcgccatat acaagtagtg ctgcgtaaaa aaatacgcaa tgagcagcga tttgcgtcgc
     1801 tggacgaact gaaagcgcag attgcgcgtg atgaattaac cgcccgcgaa ttttttgggc
     1861 taacaaaacc ggcttaagcc tgttatgtaa tcaaaccgaa acacggaacc gagaatctga
     1921 tgagtgacta taaatcaacc ctgaatttgc cggaaacagg gttcccgatg cgtggcgatc
     1981 tcgccaagcg cgaaccggga atgctggcgc gttggactga tgatgatctg tacggcatca
     2041 tccgtgcggc taaaaaaggc aaaaaaacct tcattctgca tgatggccct ccttatgcga
     2101 atggcagcat tcatattggt cactcggtta acaagattct gaaagacatt atcgtgaagt
     2161 ccaaagggct ttccggttat gactcgccgt atgtgcctgg ctgggactgc cacggtctgc
     2221 cgatcgagct gaaagtagag caagaatacg gtaagccggg tgagaaattc accgccgccg
     2281 agttccgcgc caagtgccgc gaatacgcgg cgacccaggt tgacggtcaa cgcaaagact
     2341 ttatccgtct gggcgtgctg ggcgactggt cgcacccgta cctgaccatg gacttcaaaa
     2401 ctgaagccaa catcatccgc gcgctgggca aaatcatcgg caacggtcac ctgcacaaag
     2461 gcgcgaagcc agttcactgg tgcgttgact gccgttctgc gctggcggaa gcggaagttg
     2521 agtattacga caaaacttct ccgtccatcg acgttgcttt tcaggcggtc gatcaggatg
     2581 cactgaaagc aaaatttgcc gtaagcaacg ttaacggccc aatctcgctg gtgatctgga
     2641 ccactacgcc gtggactctg cctgcgaacc gcgcaatctc tattgcacct gatttcgact
     2701 atgcgctggt gcagatcgac ggtcaggccg tgattctggc gaaagatctg gttgaaagcg
     2761 taatgcagcg tatcggcgtg accgattaca ccattctcgg cacggtaaaa ggtgcggagc
     2821 ttgagttgct gcgctttacc catccgttta tgggcttcga cgttccggca atcctcggcg
     2881 atcacgttac cctagatgcc ggtaccggtg ccgttcacac cgcgcctggc cacggcccgg
     2941 acgactatgt gatcggtcag aaatacggcc tggaaaccgc taacccggtt ggcccggacg
     3001 gcacttatct gccgggcact tatccgacgc tggatggcgt gaacgtcttc aaagcgaacg
     3061 acatcgtcgt tgcgctgctg caggaaaaag gcgcgctgct gcacgttgag aaaatgcagc
     3121 acagctatcc gtgctgctgg cgtcacaaaa cgccgatcat cttccgcgcg acgccgcagt
     3181 ggttcgtcag catggatcag aaaggtctgc gtgcgcagtc actgaaagag atcaaaggcg
     3241 tgcagtggat cccggactgg ggccaggcgc gtatcgagtc gatggttgct aaccgtcctg
     3301 actggtgtat ctcccgtcag cgcacctggg gcgtaccgat gtcactgttc gtgcacaaag
     3361 acacggaaga gctgcatccg cgtactctcg aactaatgga agaagtggca aaacgcgttg
     3421 aagttgacgg catccaggcg tggtgggatc ttgatgcgaa agagatcctc ggcgatgaag
     3481 ctgatcagta cgtgaaagtg ccggacacat tggatgtatg gtttgactcc ggatctaccc
     3541 actcttctgt tgttgacgtg cgtccggaat ttgccggtca cgcagcggac atgtatctgg
     3601 aaggttctga ccagcaccgt ggttggttca tgtcttccct aatgatctcc accgcgatga
     3661 aaggcaaagc gccgtatcgt caggtactga cccacggctt taccgtggat ggtcagggtc
     3721 gcaagatgtc taaatccatc ggcaataccg tttcgccgca ggatgtgatg aacaaactgg
     3781 gcgcggatat tctgcgtctg tgggtggcat caaccgacta caccggtgaa atggccgttt
     3841 ctgacgagat cctgaaacgt gctgccgata gctatcgtcg tatccgtaac accgcgcgct
     3901 tcctgctggc aaacctgaac ggttttgatc cagcaaaaga tatggtgaaa ccggaagaga
     3961 tggtggtact ggatcgctgg gccgtaggtt gtgcgaaagc ggcacaggaa gacatcctca
     4021 aggcgtacga agcatacgat ttccacgaag tggtacagcg tctgatgcgc ttctgctccg
     4081 ttgagatggg ttccttctac ctcgacatca tcaaagaccg tcagtacacc gccaaagcgg
     4141 acagtgtggc gcgtcgtagc tgccagactg cgctgtatca catcgcagaa gcgctggtgc
     4201 gctggatggc accaatcctc tccttcaccg ctgatgaagt gtggggctac ctgccgggcg
     4261 aacgtgaaaa atacgtcttc accggtgagt ggtacgaagg cctgtttggc ctggcagaca
     4321 gtgaagcgat gaacgatgcg ttctgggacg agctgttgaa agtgcgtggc gaagtgaaca
     4381 aagtcattga gcaagcgcgt gccgacaaga aagtgggtgg ctcgctggaa gcggcagtaa
     4441 ccttgtatgc agaaccggaa ctgtcggcga aactgaccgc gctgggcgat gaattacgat
     4501 ttgtcctgtt gacctccggc gctaccgttg cagactataa cgacgcacct gctgatgctc
     4561 agcagagcga agtactcaaa gggctgaaag tcgcgttgag taaagccgaa ggtgagaagt
     4621 gcccacgctg ctggcactac acccaggatg tcggcaaggt ggcggaacac gcagaaatct
     4681 gcggccgctg tgtcagcaac gtcgccggtg acggtgaaaa acgtaagttt gcctgatgag
     4741 tcaatcgatc tgttcaacag ggctacgctg gctgtggctg gtggtagtcg tgctgattat
     4801 cgatctgggc agcaaatacc tgatcctcca gaactttgct ctgggggata cggtcccgct
     4861 gttcccgtcg cttaatctgc attatgcgcg taactatggc gcggcgttta gtttccttgc
     4921 cgatagcggc ggctggcagc gttggttctt tgccggtatt gcgattggta ttagcgtgat
     4981 cctggcagtg atgatgtatc gctcgaaggc cacgcagaag ctaaacaata tcgcttacgc
     5041 gctgattatt ggcggcgcgc tgggcaacct gttcgaccgc ctgtggcacg gcttcgttgt
     5101 cgatatgatc gacttctacg tcggcgactg gcacttcgcc accttcaacc ttgccgatac
     5161 tgccatctgt gtcggtgcgg cactgattgt gctggaaggt tttttgcctt ctagagcgaa
     5221 aaaacaataa taaaccctgc cggatgcgat gctgacgcat cttatccggc ctacagattg
     5281 ctgcgaaatc gtaggccgga taaggcgttt acgccgcatc cggcaaaaat cctcaaatat
     5341 aagagcaaac ctgcatgtct gaatctgtac agagcaatag cgccgtcctg gtgcacttca
     5401 cgctaaaact cgacgatggc accaccgccg agtctacccg caacaacggt aaaccggcgc
     5461 tgttccgcct gggtgatgct tctctttctg aagggctgga gcaacacctg ttggggctga
     5521 aagtgggcga taaaaccacc ttctcgttgg agccagatgc ggcgtttggc gtgccgtcac
     5581 cggacctgat tcagtacttt tcccgccgtg aatttatgga tgcaggcgag ccagaaattg
     5641 gcgcaatcat gctttttacc gcaatggatg gcagtgagat gcctggcgtg atccgcgaaa
     5701 ttaacggcga ctccattacc gttgatttca accatccgct ggccgggcag accgttcatt
     5761 ttgatattga agtgctggaa atcgatccgg cattggaggc gtaacatgca gatcctgttg
     5821 gccaacccgc gtggtttttg tgccggggta gaccgcgcta tcagcattgt tgaaaacgcg
     5881 ctggccattt acggcgcacc gatatatgtc cgtcacgaag tggtacataa ccgctatgtg
     5941 gtcgatagct tgcgtgagcg tggggctatc tttattgagc agattagcga agtaccggac
     6001 ggcgcgatcc tgattttctc cgcacacggt gtttctcagg cggtacgtaa cgaagcaaaa
     6061 agccgcgatt tgacggtgtt tgatgccacc tgtccgctgg tgaccaaagt gcatatggaa
     6121 gtcgcccgcg ccagccgtcg tggcgaagag tcgattctca tcggccacgc cgggcatccg
     6181 gaagtggaag gtacaatggg ccagtacagt aacccggaag ggggaatgta tctggtcgaa
     6241 tcgccggacg atgtgtggaa actgacggtc aaaaacgaag agaagctctc ctttatgacc
     6301 cagaccacgc tgtcggtgga tgacacgtct gatgtgatcg acgcgctgcg taaacgcttc
     6361 ccgaaaattg tcggtccgcg caaagatgac atctgctacg ccacgactaa ccgtcaggaa
     6421 gcggtacgcg ccctggcaga acaggcggaa gttgtgttgg tggtcggttc gaaaaactcc
     6481 tccaactcca accgtctggc ggagctggcc cagcgtatgg gcaaacgcgc gtttttgatt
     6541 gacgatgcga aagatatcca ggaagagtgg gtgaaagagg ttaaatgcgt cggcgtgact
     6601 gcgggcgcat cggctccgga tattctggtg caaaatgtgg tggcacgttt gcagcagctg
     6661 ggcggtggtg aagccattcc gctggaaggc cgtgaagaaa acattgtttt cgaagtgccg
     6721 aaagagctgc gtgtcgatat tcgtgaagtc gattaagtca ttagcagcct aagttatgcg
     6781 aaaatgccgg tcttgttacc ggcatttttt atggagaaaa catgcgttta cctatcttcc
     6841 tcgatactga ccccggcatt gacgatgccg tcgccattgc cgccgcgatt tttgcccccg
     6901 aactcgacct gcaactgatg accaccgtcg cgggtaatgt ctcggttgag aaaaccaccc
     6961 gcaacgccct gcaactgctg catttctgga atgcggagat tccgctcgcc cagggagccg
     7021 ctgtgccgct ggtacgcgca ccgcgtgatg cagcatctgt gcacggcgaa tcgggaatgg
     7081 ctggctacga ctttgttgag cacaaccgaa agccaatcgg gataccagcg tttctggcga
     7141 ttcgtgatgc cctgatgcgt gcaccagagc ctgttaccct ggtggccatt ggcccgttaa
     7201 ccaatattgc gctgttactt tcacaatgcc cggaatgtaa accgtatatt cgtcgtctgg
     7261 tgatcatggg cggttcttct ggtcgcggca actgtacacc aaatgccgag tttaatatcg
     7321 ctgccgatcc agaagcggct gcctgtgtct tccgtagcgg aatcgaaatc gtcatgtgcg
     7381 gtctggatgt caccaatcag gcaatattaa cccctgacta tctcgctact ttgccggagt
     7441 tgaatcgcac cggaaaaatg cttcatgcac tgtttagcca ctaccgtagc ggcagtatgc
     7501 aaagcggtct gcgaatgcac gatctctgcg ccatcgcctg gctggtgcgc ccggacttat
     7561 tcaccctcaa gccgtgtttt gtcgcggtag aaactcaagg cgaatttacc tcaggcacga
     7621 cggtggttga tatcgacggt tgcctgggca aaccagccaa tgtaaaggtg gcattggatc
     7681 tggatgtgaa aggcttccag cagtgggtgg ctgaggtgct ggctctggcg tcgtaacctg
     7741 tcacatgtta tcggcatgca gtcattcatc gactcatgcc tttcactgat atccctccct
     7801 gtttatcatt aatttctaat tatcagcgtt tttggctggc ggcgtagcga tgcgctggtt
     7861 actctgaaaa cggtctatgc aaattaacaa aagagaatag ctatgcatga tgcaaacatc
     7921 cgcgttgcca tcgcgggagc cggggggcgt atgggccgcc agttgattca ggcggcgctg
     7981 gcattagaag gcgtgcagct gggcgcggcg ctggagcgcg aaggatcttc tttactgggc
     8041 agcgacgccg gtgagctggc tggtgcaggg aaaacaggcg ttaccgtgca aagcagcctc
     8101 gatgcggtaa aagatgattt tgatgtgttt atcgatttta cccgtccgga aggtacgctg
     8161 aaccatctcg ctttttgtcg ccagcatggc aaagggatgg tgatcggcac tacggggttt
     8221 gacgaagccg gtaaacaggc gattcgtgac gccgccgccg atattgcgat tgtctttgcc
     8281 gccaatttta gcgttggcgt taacgtcatg cttaagctgc tggagaaagc agccaaagtg
     8341 atgggtgact acaccgatat cgaaattatt gaagcacatc atagacataa agttgatgcg
     8401 ccgtcaggca ccgcactggc aatgggagag gcgatcgccc acgcccttga taaagatctg
     8461 aaagattgcg cggtctacag tcgtgaaggc cacaccggtg aacgtgtgcc tggcaccatt
     8521 ggttttgcca ccgtgcgtgc aggtgacatc gttggtgaac ataccgcgat gtttgccgat
     8581 attggcgagc gtctggagat cacccataag gcgtccagcc gtatgacatt tgctaacggc
     8641 gcggtaagat cggctttgtg gttgagtggt aaggaaaatg gtctttttga tatgcgagat
     8701 gtacttaatc tcaataattt gtaaccacaa aatatttgtt atggtgcaaa aataacacat
     8761 ttaatttatt gattataaag ggctttaatt tttggccctt ttatttttag tgttatgttt
     8821 ttaaatattc tataagtgcc aaaaattaca tgttttgtct tctatttttg ttgttttaat
     8881 gtaaattttg accatttggt ccactttttc ctgctcgttt ttatttcatg caatcttctt
     8941 gctgcgcaag cgttttccag aacaggttat atgatctttt tgtcgcttaa tgcctgtaaa
     9001 atatgcatga gccacaaaat aatataaaaa ataccgccat taagttgact tttagcgccc
     9061 atatctccag aatgccgccg tttgccagaa attcgtcggt aagcagattt gcattgattt
     9121 acgtcatcat tgtgaattaa tatgcaaata aagtgagtga atattctctg gagggtgttt
     9181 tgattaagtc agcgctattg gttctggaag acggaaccca gtttcacggt cgggccatag
     9241 gggcaacagg ttcggcggtt ggggaagtcg ttttcaatac ttcaatgacc ggttatcaag
     9301 aaatcctcac tgatccttcc tattctcgtc aaatcgttac tcttacttat ccccatattg
     9361 gcaatgtcgg cactaatgac gccgatgaag aatcttctca ggtacatgca caaggtctgg
     9421 tgattcgcga tctgccgctg attgccagca acttccgtaa taccgaagac ctctcttctt
     9481 acctgaagcg ccataacatc gtggcgattg ccgatatcga tacccgtaag ctgacgcgtt
     9541 tactgcgcga gaaaggcgca cagaatggct gcattatcgc aggcgataac ccggatgcgg
     9601 cgttggcgtt agaaaaagcc cgcgcgttcc caggtctgaa tggcatggat ctggcaaaag
     9661 aagtgaccac cgcagaagcc tatagctgga cacaagggag ctggacgttg accggcggcc
     9721 tgccagaagc gaaaaaagaa gacgagctgc cgttccacgt cgtggcttat gattttggtg
     9781 ccaagcgcaa catcctgcgg atgctggtgg atagaggctg tcgcctgacc atcgttccgg
     9841 cgcaaacttc tgcggaagat gtgctgaaaa tgaatccaga cggcatcttc ctctccaacg
     9901 gtcctggcga cccggccccg tgcgattacg ccattaccgc catccagaaa ttcctcgaaa
     9961 ccgatattcc ggtattcggc atctgcctcg gtcatcagct gctggcgctg gcgagcggtg
    10021 cgaagactgt caaaatgaaa tttggtcacc acggcggcaa ccatccggtt aaagatgtgg
    10081 agaaaaacgt ggtgatgatc accgcccaga accacggttt tgcggtggat gaagcaacat
    10141 tacctgcaaa cctgcgtgtc acgcataaat ccctgttcga cggtacgtta cagggcattc
    10201 atcgcactga taaaccggcg ttcagcttcc agggtcaccc ggaagccagc cctgggccac
    10261 acgacgcagc gccgttgttc gaccacttta tcgagttaat tgagcagtac cgtaaaaccg
    10321 ctaagtaatc aggagtaaaa gagccatgcc aaaacgtaca gatataaaaa gtatcctgat
    10381 tctgggtgcg ggcccgattg ttatcggtca ggcgtgtgag tttgactact ctggcgcgca
    10441 agcatgtaaa gccctgcgcg aagagggtta ccgcgtcatt ctggtgaact ccaacccggc
    10501 gaccatcatg accgacccgg aaatggccga tgcgacctac atcgagccga ttcactggga
    10561 agtagtacgc aagattattg aaaaagagcg cccggacgcg gtgctgccaa cgatgggcgg
    10621 tcagacggcg ctgaactgcg cgctggagct ggagcgtcag ggcgtgttgg aagagttcgg
    10681 cgtgactatg attggtgcga ccgccgatgc gattgataaa gcagaagacc gccgtcgttt
    10741 cgacgtagcg atgaagaaaa ttggtctgga aaccgcgcgt tccggtatcg cacacacgat
    10801 ggaagaagcg ctggcggttg ccgctgacgt gggcttcccg tgcattattc gcccatcctt
    10861 taccatgggc ggtagcggcg gcggtatcgc ttataaccgc gaagagtttg aagaaatttg
    10921 cgcccgcggt ctggatctct ccccaaccaa agagctgctg attgatgagt cgctgatcgg
    10981 ctggaaagag tacgagatgg aagtggtgcg tgataaaaac gacaactgca tcatcgtctg
    11041 ctctatcgaa aactttgatg cgatgggcat ccataccggt gactccatca ctgtcgcgcc
    11101 agcccaaacg ctgaccgaca aagaatatca aatcatgcgt aacgcctcga tggcggtgct
    11161 gcgtgaaatc ggcgttgaaa ccggtggttc caatgtccag tttgcggtga acccgaaaaa
    11221 cggtcgcctg attgttatcg aaatgaaccc acgcgtgtcc cgttcttcgg cgctggcgtc
    11281 gaaagcgacc ggtttcccga ttgctaaagt ggcggcgaaa ctggcggtgg gttacaccct
    11341 cgacgaactg atgaacgaca tcactggcgg acgtactccg gcctccttcg agccgtccat
    11401 cgactatgtg gttactaaaa ttcctcgctt caacttcgaa aaattcgctg gtgctaacga
    11461 ccgtctgacc actcagatga aatcggttgg cgaagtgatg gcgattggtc gcacgcagca
    11521 ggaatccctg caaaaagcgc tgcgcggcct ggaagtcggt gcgactggat tcgacccgaa
    11581 agtgagcctc gatgacccgg aagcgttaac caaaatccgt cgcgaactga aagatgctgg
    11641 cgcagagcgt atctggtaca tcgccgatgc cttccgtgcg ggcctgtctg tggacggcgt
    11701 gttcaacctg accaatattg accgctggtt cctggtacag attgaagaac tggtgcgtct
    11761 ggaagagaaa gtagcggaag tgggcatcac tggcctgaac gctgaattcc tgcgccagct
    11821 gaaacgcaaa ggctttgccg atgcgcgtct ggcaaaactg gcgggcgtgc gcgaagcgga
    11881 aatccgcaag ctgcgcgacc agtatgacct gcacccggtt tataagcgcg tggatacctg
    11941 tgcggcagag ttcgccaccg acaccgctta catgtactcc acttatgaag aagagtgcga
    12001 agcgaatccg tctaccgacc gtgaaaaaat catggttctc ggcggcggcc cgaaccgtat
    12061 cggtcagggt atcgaattcg actactgctg tgtacacgcc tcgctggcgc tgcgcgaaga
    12121 cggttacgaa accattatgg ttaactgtaa cccggaaacc gtctccaccg actacgacac
    12181 ttccgatcgc ctctacttcg agccggtaac tctggaagat gtgctggaaa tcgtgcgtat
    12241 tgagaagccg aaaggcgtta tcgttcagta cggcggtcag accccgctga aactggcgcg
    12301 cgcgctggaa gctgctggcg taccggttat cggcaccagc ccggatgcta tcgaccgtgc
    12361 agaagaccgt gaacgcttcc agcatgcggt tgagcgtctg aaactgaaac aaccggcgaa
    12421 cgccaccgtt accgctattg aaatggcggt agagaaggcg aaagagattg gctacccact
    12481 ggtggtacgt ccgtcttacg ttctcggcgg tcgggcgatg gaaatcgtct atgacgaagc
    12541 tgacctgcgt cgctacttcc agacggcggt cagcgtgtct aacgatgcgc cagtgttgct
    12601 ggatcacttc cttgatgacg cggtagaagt tgacgtggat gccatctgcg acggcgaaat
    12661 ggtgctgatt ggcggcatca tggagcacat cgagcaggcg ggcgtgcact ccggtgactc
    12721 cgcatgttct ctgccagcct acaccttaag tcaggaaatt caggatgtga tgcgccagca
    12781 ggtgcagaaa ctggccttcg aattgcaggt gcgcggtctg atgaacgtgc agtttgcggt
    12841 gaaaaacaac gaagtctacc tgattgaagt taacccgcgt gcggcgcgta ccgttccgtt
    12901 cgtctccaaa gccaccggcg taccgctggc aaaagtggcg gcgcgtgtga tggctggcaa
    12961 atcgctggct gagcagggcg taaccaaaga agttatcccg ccgtactact cggtgaaaga
    13021 agtggtgctg ccgttcaata aattcccggg tgttgacccg ctgttagggc cagaaatgcg
    13081 ctccaccggg gaagtgatgg gcgtgggccg caccttcgct gaagcgtttg ccaaagcgca
    13141 gctgggcagc aactccacca tgaagaaaca cggtcgtgcg ttgctttccg tgcgcgaagg
    13201 cgataaagaa cgcgtggtgg acctggcggc aaaactgctg aaacagggct tcgagctgga
    13261 tgcgacccac ggcacggcga ttgtgctggg cgaagcgggc attaatccgc gtctggtgaa
    13321 caaggtgcat gaaggccgtc cgcacattca ggaccgcatc aagaatggcg aatataccta
    13381 catcatcaac accacttcag gccgtcgcgc gattgaagac tcccgcgtga tccgtcgcag
    13441 tgcgctgcaa tataaagtgc attatgacac caccctgaac ggtggtttcg ctaccgcgat
    13501 ggcgctgaat gccgacgcga ccgaaaaagt aatttcggtg caggaaatgc acgcgcagat
    13561 caaataatag cgtgtcatgg cggatatttt tcatccgcta atttgatcga ataagtaata
    13621 cggttctctg atgaggaccg ttttttttgc ccattaatta aatcttttgg ggaatcgata
    13681 tttttgatga cataagcagg atttagctca cact
//
LOCUS       DAZPJR010000110        13489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPJR010000110 DAZPJR010000000
VERSION     DAZPJR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.111"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(36..119)
                     /locus_tag="ACHVB7_002951"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="ACHVB7_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHVB7_002952"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHVB7_002952"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR1914878.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHVB7_002953"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHVB7_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR1914879.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHVB7_002954"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHVB7_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR1914880.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="ACHVB7_002955"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="ACHVB7_002955"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR1914881.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="ACHVB7_002956"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="ACHVB7_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR1914882.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="ACHVB7_002957"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="ACHVB7_002957"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR1914883.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="ACHVB7_002958"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="ACHVB7_002958"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR1914884.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="ACHVB7_002959"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="ACHVB7_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHVB7_002960"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHVB7_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR1914885.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="ACHVB7_002961"
     CDS             complement(12425..12664)
                     /locus_tag="ACHVB7_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR1914886.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHVB7_002962"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHVB7_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR1914887.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13041..13328
                     /locus_tag="ACHVB7_002963"
     CDS             13041..13328
                     /locus_tag="ACHVB7_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR1914888.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3519 a         3318 c         3468 g         3184 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    13021 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    13081 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    13141 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    13201 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    13261 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    13321 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    13381 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    13441 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatatt
//
LOCUS       DAZPJR010000111        13317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPJR010000111 DAZPJR010000000
VERSION     DAZPJR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.112"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHVB7_002964"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHVB7_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR1914889.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHVB7_002965"
     CDS             433..621
                     /locus_tag="ACHVB7_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR1914890.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHVB7_002966"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHVB7_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR1914891.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHVB7_002967"
     CDS             816..989
                     /locus_tag="ACHVB7_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR1914892.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHVB7_002968"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHVB7_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHVB7_002969"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHVB7_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR1914893.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHVB7_002970"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHVB7_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR1914894.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHVB7_002971"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHVB7_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR1914895.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHVB7_002972"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHVB7_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR1914896.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHVB7_002973"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHVB7_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHVB7_002974"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHVB7_002974"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1914897.1"
                     /translation="MFSRELDDEQLTQIASAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHVB7_002975"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHVB7_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR1914898.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHVB7_002976"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHVB7_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR1914899.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13317
                     /locus_tag="ACHVB7_002977"
                     /pseudo
     CDS             12531..13317
                     /locus_tag="ACHVB7_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3300 a         3309 c         3441 g         3267 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgacattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttatt
//
LOCUS       DAZPJR010000112        13265 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPJR010000112 DAZPJR010000000
VERSION     DAZPJR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.113"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(898..1989)
                     /gene="ychF"
                     /locus_tag="ACHVB7_002978"
     CDS             complement(898..1989)
                     /gene="ychF"
                     /locus_tag="ACHVB7_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR1914900.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2106..2690)
                     /gene="pth"
                     /locus_tag="ACHVB7_002979"
     CDS             complement(2106..2690)
                     /gene="pth"
                     /locus_tag="ACHVB7_002979"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR1914901.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2967..3245
                     /gene="ychH"
                     /locus_tag="ACHVB7_002980"
     CDS             2967..3245
                     /gene="ychH"
                     /locus_tag="ACHVB7_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR1914902.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3300..4979)
                     /gene="dauA"
                     /locus_tag="ACHVB7_002981"
     CDS             complement(3300..4979)
                     /gene="dauA"
                     /locus_tag="ACHVB7_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR1914903.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5103..6050)
                     /gene="prs"
                     /locus_tag="ACHVB7_002982"
     CDS             complement(5103..6050)
                     /gene="prs"
                     /locus_tag="ACHVB7_002982"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR1914904.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6201..7052)
                     /gene="ispE"
                     /locus_tag="ACHVB7_002983"
     CDS             complement(6201..7052)
                     /gene="ispE"
                     /locus_tag="ACHVB7_002983"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR1914905.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7052..7675)
                     /gene="lolB"
                     /locus_tag="ACHVB7_002984"
     CDS             complement(7052..7675)
                     /gene="lolB"
                     /locus_tag="ACHVB7_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR1914906.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7889..9145
                     /gene="hemA"
                     /locus_tag="ACHVB7_002985"
     CDS             7889..9145
                     /gene="hemA"
                     /locus_tag="ACHVB7_002985"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR1914907.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9187..10269
                     /gene="prfA"
                     /locus_tag="ACHVB7_002986"
     CDS             9187..10269
                     /gene="prfA"
                     /locus_tag="ACHVB7_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR1914908.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10269..11102
                     /gene="prmC"
                     /locus_tag="ACHVB7_002987"
     CDS             10269..11102
                     /gene="prmC"
                     /locus_tag="ACHVB7_002987"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1914909.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11099..11491
                     /gene="sirB2"
                     /locus_tag="ACHVB7_002988"
     CDS             11099..11491
                     /gene="sirB2"
                     /locus_tag="ACHVB7_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR1914910.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11495..12303
                     /gene="sirB1"
                     /locus_tag="ACHVB7_002989"
                     /pseudo
     CDS             11495..12303
                     /gene="sirB1"
                     /locus_tag="ACHVB7_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
     gene            12339..13193
                     /gene="kdsA"
                     /locus_tag="ACHVB7_002990"
     CDS             12339..13193
                     /gene="kdsA"
                     /locus_tag="ACHVB7_002990"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR1914911.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3346 a         3286 c         3394 g         3239 t
ORIGIN      
        1 tttaattcag cgagacgatc tgctttggta ttcccattgt gttgtttgat gcccatacca
       61 ctcctatata gtacccagac tatgggtcta tggattttgt cattttgttt aaatcaatga
      121 ataatatcct cttatcatca tgatatttgt atggtgcaat catgatgagc ttctcagaga
      181 ataattctct attaccgggc aaaatcagga acacaggaat taatatatat ttaacatccc
      241 ccttacaaag gtgagtttat atcgagttat tccctaagtt gtcgttactt attccaattc
      301 gttactataa atatggcaaa aatattacaa caaacagcgg ttaaaaaatt tggtgaagat
      361 tttttgtaat aatcctaatt agttatggga atttcgcttg atgtatctgc ataacaaaaa
      421 caatatgcgc catttttgca ttactaataa gaaaaagcaa aaaacatcga tattcatttt
      481 tggtcaatgg gttttctggc ctatcgttgt tattgtgcta ctggttatca ttttcatctt
      541 gccaggatcg cacgtaatca cgttattcgg ggactttatc tgcgggatat tacccttcag
      601 gtaagtcctt ttctcgccag catattggag cgtgatcaat attgatcagc tgtgaagggg
      661 gtgatgtgta gttgaacaaa aacttacgtt atatcactac taatgagata tccgtcatct
      721 gacactgatg tctttaatta ttctcgtggt tcactgtagg caataagcac aaaagtgtag
      781 gatgttacaa gaatgattag gactcggtga aatgaaaaat ccacgcaatt gcgtggattt
      841 tatatacttt tgcgctcttc atgagattta gcgaaacctc atgagacaat aaattaatta
      901 gacgttgaaa aggaagttca tcacatcgcc atctttaacg atgtaatctt taccttctgc
      961 acgcattttg cctgcttctt tcgcgccttg ttcacctttg taagtgatga aatcttcaaa
     1021 cgagatggtt tgtgcacgga taaagccttt ttcaaaatca gtatggattt tgcccgctgc
     1081 ctgcggcgcg gttgctccaa ccggaatggt ccatgcacgc acttctttca ccccagcggt
     1141 gaagtaagtt tgcaggttca gcagcttata accgacacgg atcacacggt tcaggcccgg
     1201 ttcttccagc ccaagctcct gcataaactc gtcacgttct tcgtcgtcca gttcggcaat
     1261 gtctgcttca acagcagcac aaaccggaac cacaacagaa ccttctttcg ccgcgatttc
     1321 acgcacctgg tcaagatatg ggttgttttc aaaaccgtct tcgttgacgt tggcgatgta
     1381 cattgttggt ttcagcgtca ggaagctcag gtaacgaata gccgctttct cttcagcgct
     1441 taaatccagc gcgcgcagca tacctgcgtt ttccagctgg ggcaggcatt tttctaggac
     1501 cgccagctca gctttcgcgt ctttatcgcc acctttggct ttcttctgta cgcgatgaat
     1561 cgcacgttcg caggtgtcga ggtctgccag cgccagttcg gtgttgataa cttcaatatc
     1621 gtcagccggg ttaactttgc cggaaacgtg aatgatgttg tcattttcaa agcagcgaac
     1681 aacgtgaccg atcgcttcgg tttcacggat gttggtcagg aactggttac ccagaccttc
     1741 gcctttcgat gcgcctttta ccagaccggc gatatcgaca aattccatgg tcgtgggaag
     1801 cgtacgttgc ggttttacga tttcagccag ttgatccagg cgagggtcag gcattggtac
     1861 gacgcctgtg ttcggctcaa tggtgcagaa tggaaagttg gccgcttcaa taccggcttt
     1921 ggtcagcgcg ttgaacaggg tagatttccc gacgttgggc aaaccgacga taccgcattt
     1981 gaatcccatg atttaaatca ccttaatatc ttaataatca acctgttatt gctaacagat
     2041 tgcagaaatg gaaataactt tgcctattat acacggcact cggcaaaaat gccgcagaca
     2101 acgacttatt gcgctttaaa ggcgtgcaat cggttcgttg ctttggtcaa gccatctgta
     2161 aaccacattt cagtacaacg cgccgcttcg tcgatggctt catcaattaa cttctgttca
     2221 ctaacaggcg gtttgcctaa cacaaaaccg acaactttat ttttatcgcc aggatgaccg
     2281 attccgatgc gtaaacggtg aaagttaggg ttattaccca atttactgat gatgtctttc
     2341 agtccattgt gaccaccatg gccaccgccc aatttaaatt tggcgacgcc aggaggcaga
     2401 tccagttcgt cgtgggccac cagaatttcg tccggattaa tgcggaaaaa actggccatc
     2461 gccgcaacgg ctttgccgct gagattcata aatgtagtcg ggactaacag gcggacatct
     2521 tcgcctccaa gagtgactcg cgaagtataa ccaaagaatt tagcctcttc gcgcagcgga
     2581 gcgcgcaaac gctctgccag taagtcaacg aaccaggcac cagcattatg tcgcgttgcg
     2641 gcgtattcag caccggggtt cgccaggccg acaatcaatt taatcgtcac gttttttgtc
     2701 ctgagtgtgt acataactgg cgcgtagttt actggttgcg gccccgcttg acaaaaaact
     2761 gcgtatcaaa tgcagataac gtaataattg cctgagtgga ctattagaaa gtcaaggtgt
     2821 tcaggcgttt atttgtaaag ttttgttgaa ataagggttg taattgtgat cacgcccgca
     2881 cataacccac agggtgttgt ctatacttta cacataagga agaggggtat tccctgttac
     2941 aacccagaaa gttccggagg tgacatatga aacgcaaaaa cgcttcgtta ctcggtaacg
     3001 tgctcatggg gttggggctg gtggtaatgg tggtcggcgt ggggtattca atcctcaacc
     3061 agttaccaca gtttaatatg ccccagtatt tcgcacatgg tgcagtgcta agtattttcg
     3121 tcggtgccat tctctggctg gcgggtgccc gtgttggcgg gcatgaacag gtgtgcgacc
     3181 gttactggtg ggttcgtcac tatgacaaac gttgccgccg tagcgataat cgccgtcata
     3241 gctaacaaat aatgcagttt gctgaccagt cagtttgcgc tgactggtca atctcgtact
     3301 tataaatccg ccatcgccgc gcgacgattc gggaagaacg cgaggcgtcc cgggatcggt
     3361 tgaatgcctg cgcgagccat agtgcgcagt ggctggaatt ccacgttgca cacgcgcagt
     3421 tcacatcctt cgggcagacg cttcacaaaa cgctggaacg catcaagacc accagcatca
     3481 agtaccggaa cggcatccca cttcagaatc acaatccgtt tgccttcaag acgtgactcc
     3541 aggtccgtga acaagccttc agcagcagca aaaaacagcg ggccaataac gcgcagtacc
     3601 agaacatcgt ctggaacatc tacgactacc ggtgccaggc gagtcatacg tgcgatacga
     3661 cgcataaaca gcagcgatgc cagcacgatc cccacgctga tggcaataac catatcaaac
     3721 agcacggtca gcgacatgca cagcagcatg acaatgatgt catctttcgg tgcatgacgc
     3781 agcaagtcga ccactttatg cgcttcactc atgttccacg ccaccatcaa cagcagggct
     3841 gccatagcgg aaagcggcag ccaggagagc agcggtgcca gtaccagcag ggcaagaata
     3901 accagaatag agtggatcac cgccgagata ggggaagttg ccccggcacg gacgttagcg
     3961 gcagaacgcg cgatggcagc tgtagcggta ataccaccaa agaacggagc gataatattc
     4021 cccagtccct gtccaaccag ttcgctgttc gccttgtgtt tcgtcccggt cataccatcc
     4081 agtaccacgg cgcagagcag agattcgatt gcgccgagca ttgccattga gaatgccgca
     4141 ggcagcagtg tgcgaataga atcccaggtt agcgtgaatt ctgaattagg cagatcccac
     4201 ggcagcacca gttgcggcag cagttgcgga ataccgttac cctgagaacc atcggccagg
     4261 acgtagtgga attgcgaacc gatggtagca acatgtccgc cgagcaggtt aacaatcccc
     4321 atcaccgcgc aaccagccag caaggccgga aggtgaccgg gtaaacgaat gcccagacgc
     4381 ggccaaaaaa caagaatacc tagcgtcaca atgccaatgg cagcatcacc cacattaatg
     4441 gtcggcagcg ccataaataa tgcgccgact ttttgtagat aatgttccgg gacatgggcc
     4501 atttgcagac cgagaaaatc tttaatctgc atggtaccga tggtgatccc gatacccgag
     4561 gtgaaaccta aggtgacgga aaccggaata tactcaatca ggcgaccaaa gcgtgccaga
     4621 cccatcagaa tcaaaaagat ccccgacagc aaggtcgcaa ccagcagtcc tgccagtcca
     4681 aactgttgcg acacgggata gagaattacc acaaatgccg cagtcggacc ggaaacgcta
     4741 aagcgtgacc cacccgtcag agcaatgaca atccccgcaa cagctgcggt atataaaccg
     4801 tactggggtg ccacaccact accaatagcc aacgccatcg ccagcgggat agcaataatc
     4861 ccgacggtta tcccggcaat caggtcacgg gtaaaccgtg cggcagtata tttttctttc
     4921 cagcaagcgt cgatcagagc gcggaaaggc atcacatgtg aggaaaatat tttgttcaca
     4981 ataatgtttc atccgtgagc gcatcatctg tcaactaaat ggcaggtgaa ggaggcatag
     5041 gtcatacaaa tggatattac agacaaaaaa acccgccgca gcgggtcttt gagcgggttc
     5101 gattagtgtt cgaacatggc agagatcgat tcttcgttgc tgatacgacg aatcgcttcg
     5161 gccagcatac ctgacagggt cagagtacgc acgttcggca gtgatttgat ttcatcgctc
     5221 agcggaatgg tatcgcagac aacgacttca tcaattacag agttacgcag gttgttcgcc
     5281 gcgttgccag agaagatcgg gtgagtcgcg tacgcaaata cacgtttagc accacgttct
     5341 ttcagagctt cagcagcttt acacagcgta ccgccagtgt cgatcatatc atcgaccagt
     5401 acgcagtcac gacctgcaac gtcaccgatg atatgcatca cctgggaaac gttcgcacgc
     5461 ggacgacgtt tgtcgatgat tgccatatcg gtatcgttca gcagcttagc gatagcgcgg
     5521 gcacgcacaa cgccgccgat gtccggagaa accacaattg ggttatccag attcagctgc
     5581 agcatgtctt ccagcaggat cgggctacca aatacgttat caaccggaac gtcgaagaaa
     5641 ccctgaatct gttcagcgtg cagatccact gtcagcacac ggtcaacacc gacgctggag
     5701 aggaagtctg caaccacttt cgcagtgatt ggtacacgag cggaacggac gcgacggtcc
     5761 tggcgcgcat agccaaagta ggggataaca gcggtgatac gacctgcgga agcacgacgc
     5821 agggcatcaa ccataacgac taattccatc aggttgtcgt tagtaggggc acaagtggac
     5881 tggatgatga aaatatcacc accgcgtaca ttttcgttga tttgtacact gacttcgccg
     5941 tcgctaaagc gacctacagc ggcgtcgccg agtgaagtgt acaggcggtt ggcaatacgt
     6001 tgtgctagtt ccggggtggc gttaccagca aaaagcttca tatcaggcac gagaagaacc
     6061 tcaggcatgc gtccattggt ggaaagaatc tgccgaaaac tgtgcgggcc aggcatgatc
     6121 ctttccaggc ggtgtattaa agagcgcgat gcaacgtctg gaacaaggtg acgttgtcac
     6181 cgaaactcag cttgcccggc ttaaagcatg gctctgtgca atggggaaag attaacacct
     6241 ttcgccacaa agccattgag ccattccggg gcttgctcta gcacctggcg ggcttcagac
     6301 tctgtatcaa attcagcaaa gacacaggcc cctgtcccag tcaggcgcga cggggcgtat
     6361 tctaacagcc aggaaagcac cgcatcaacc tcgcgaaaac gttttcttgc gataacctcg
     6421 caatcattgc tgaattcaca ttttagcaac gtttctattg acctttttgg cgtattgcgc
     6481 gggagttcag gatctttaaa aatcactggt gtcggaatac ttacaccagg gtgcgccacc
     6541 agataccact tctctggcgg atccaccggc gttagtattt caccaacgcc ttcggcaaac
     6601 gcggcatgcc cccgaacaaa gacaggaaca tctgcgccca gcgtcagccc catttccgcc
     6661 agctcatcca tgcttagccc gcattgccag agatgattta atgccaccag gaccgtcgcg
     6721 gcattggatg aaccaccgcc gagaccgccg cccatcggca aacgcttgtc aatgctgata
     6781 ttcgcaccgc ttcccgtcgg aagacgcccg ctgtctgccg cagttttcat caacaaccgc
     6841 gctgcgcgaa cgatcaggtt atcttcatgt tccacgcctt caacgggcgt taacagacga
     6901 atatccccat cgtcacgaag ctcaatgctg atggtgtcgc cgtaatcaag aaactgaaac
     6961 agcgtttgca gcgtgtggta accatccgca cgctgaccgg taatgtataa aaacagatta
     7021 agttttgccg gagagggcca ctgtgtccgc attatttcac tatccagtta tccattttta
     7081 acttgatgcg ttgaccaccg tcggtgagtt ccatattggc tggcatcgca ggttgcgttt
     7141 tggtgtcata accaccataa acaaccttcc agtttttgcc attctggctg taggtaattt
     7201 cgctcaggcg gtactggtcg tccagtttgt agtcggttgc atcacccggt aaacctaaaa
     7261 tccattggcg caagctgttg agcggaattg gcattccggt caatttgcca atcatctctt
     7321 cggcgtcatc ggcggtataa cgctgacctt tattgtcgac taactgcacg ttacccggtt
     7381 gagcattcag ctccagttcc gtgctgccca atgggttagt gagcagcaga cggtagcgat
     7441 cctggccggt ttgctgccag aaaaagcggg cgtacacttt ttgttggtca gaaatataag
     7501 cgaacgcgcc gcgagtctga tactgattaa gattgcgcac gtcttgctga tgctgacgcc
     7561 attgtggcga atccgggctt ttgccaggac ctttgggcgt ggtaacggaa caggcagtga
     7621 gcacaagagc agccagcggt agcaggcgga taagacgaaa atcgggctgg ggcatagtga
     7681 tgacaagtcc ttgagatacg ttgcagttat aacccttaat gctagcgtta ccgtccgcta
     7741 tcgtctatgt tcaagttgtc ttaattgcca gaatctaacg gctttcggca attactccaa
     7801 aagggggcgc tctcttttat tgatcttacg catcctgtat gatgcaagca gactaaccat
     7861 atcaacgttg gtattatttc ccgcagacat gaccctttta gcactcggta tcaaccataa
     7921 aacggcacct gtatcgctgc gagaacgtgt atcgttttcg ccggataagc tcgatcaggc
     7981 gcttgacagc ctgcttgcgc agccgatggt gcagggcggc gtggtgctgt cgacgtgcaa
     8041 ccgcacggaa ctttatctta gcgttgaaga gcaggataac ctgcaagagg cgttaatccg
     8101 ctggctttgc gattatcaca atcttaatga agaagatctg cgtaaaagcc tctactggca
     8161 tcaggataac gacgcggtta gccatttaat gcgtgttgcc agcggcctgg attcactggt
     8221 tcttggggag ccgcagatcc tcggtcaggt taaaaaagcg tttgccgatt cgcaaaaagg
     8281 tcatatgaag gccagcgaac tggaacgcat gttccagaaa tctttctctg tagcgaaacg
     8341 cgttcgcact gaaacagata tcggtgccag tgctgtgtct gtcgcttttg cggcttgtac
     8401 gctggcgcgg cagatctttg aatcgctctc tacggtcaca gtgttgctgg taggcgcggg
     8461 cgaaaccatc gagctggtag cgcgtcatct gcgcgaacat aaagtacaga agatgattat
     8521 cgccaaccgc actcgcgaac gtgcccaaat tctggcagat gaagttggcg cggaagtgat
     8581 tgccctgagt gagatcgacg aacgtctgcg cgaagccgat atcatcatca gttccaccgc
     8641 cagcccgtta ccgattatcg ggaaaggcat ggtggagcgc gcattaaaaa gtcgtcgtaa
     8701 ccaaccaatg ctgttggtgg atattgccgt tccgcgcgat gttgagccgg aagttggcaa
     8761 actggcgaat gcttatcttt atagtgtgga cgatctgcaa agcatcattt cgcacaacct
     8821 ggcgcagcgt aaagccgcag cggttgaggc ggaaactatt gtcgctcagg aaaccagcga
     8881 atttatggcg tggctgcgag cacaaagcgc cagcgaaacc attcgcgagt atcgcagcca
     8941 ggcagagcac gttcgcgatg agttaaccgc caaagcgtta gcggcccttg agcagggcgg
     9001 cgacgcgcaa gccattatgc aggatctggc atggaaactg actaaccgct tgatccatgc
     9061 gccaacgaaa tcacttcaac aggccgcccg tgacggggat aacgaacgcc tgaatattct
     9121 gcgcgacagc ctcgggctgg agtagcagta catcattttc tttttttaca gggtgcattt
     9181 acgcctatga agccttctat cgttgccaaa ctggaagccc tgcatgaacg ccatgaagaa
     9241 gttcaggcgt tgctgggtga cgcgcaaact atcgccgacc aggaacgttt tcgcgcatta
     9301 tcacgcgaat atgcgcagtt aagtgatgtt tcgcgctgtt ttaccgactg gcaacaggtt
     9361 caggaagata tcgaaaccgc acagatgatg ctcgatgatc ctgaaatgcg tgagatggcg
     9421 caggatgaac tgcgcgaagc taaagaaaaa agcgagcaac tggaacagca attacaggtt
     9481 ctgttactgc caaaagatcc tgatgacgaa cgtaacgcct tcctcgaagt ccgagccgga
     9541 accggcggcg acgaagcggc gctgttcgcg ggcgatctgt tccgtatgta cagccgttat
     9601 gccgaagccc gccgctggcg ggtagaaatc atgagcgcca gcgagggtga acatggtggt
     9661 tataaagaga tcatcgccaa aattagcggt gatggtgtgt atggtcgtct gaaatttgaa
     9721 tccggcggtc atcgcgtgca gcgtgttcct gctacggaat cgcagggtcg tattcatact
     9781 tctgcttgta ccgttgcggt aatgccagaa ctgcctgacg cagaactgcc ggacatcaac
     9841 ccagcagatt tacgcattga tactttccgc tcgtcagggg cgggtggtca gcacgttaac
     9901 accaccgatt cggcaattcg tattactcac ttgccgaccg ggattgttgt tgaatgtcag
     9961 gacgaacgtt cacaacataa aaacaaagct aaagcacttt ctgtacttgg tgctcgtatc
    10021 cacgctgcag aaatggcaaa acggcaacag gccgaagcgt ctacccgtcg taacctgctg
    10081 gggagtggcg atcgcagcga ccgtaaccgt acttacaact tcccgcaggg gcgcgttacc
    10141 gatcaccgca tcaacctgac gctctaccgt ctggatgaag tgatggaagg taagctggat
    10201 atgctgattg aaccgattat ccaggaacat caggccgacc aactggcggc gttgtccgag
    10261 caggaataat ggaatatcaa tactggttac gtgaagcaat aagccaactt caggcgagcg
    10321 aaagcccacg ccgtgacgct gaaatcttat tggagtatgt cactggcagg gggcggactt
    10381 ttattctggc ctttggtgaa acgcagctga ctgacgaaca atgtcagcaa cttgatgcgc
    10441 tactgacacg tcgtcgcgat ggtgaaccca ttgctcattt aaccggggtg cgagaattct
    10501 ggtcgttgcc gttatttgtt tcgccagcga ccttaattcc gcgcccggat acggagtgtc
    10561 tggtggagca ggcactggcg cggttgcctg aacaaccttg ccgtattctc gatctcggga
    10621 cgggtaccgg ggcgattgcg cttgcgctgg ctagcgagcg cccggactgc gaaattatcg
    10681 ctgtagatcg tatgcctgat gctgtctccc tggcacaacg taatgcccag catctggcga
    10741 tcaaaaatat ccacattctg caaagcgact ggtttagcgc gctagccggg cagcagtttg
    10801 cgatgattgt cagcaatccg ccgtatattg acgagcagga cccacatctt caacaaggcg
    10861 atgtccgctt tgagccgctc actgcgctgg ttgcggcaga cagtggaatg gcagacatcg
    10921 tgcatatcat cgaacagtcg cgtaacgcgc tggtatccgg cggctttctg cttctggaac
    10981 atggctggca gcagggcgaa gcggtgcgac aagcatttat cctcgcgggg tatcatgacg
    11041 tcgaaacctg ccgtgactat ggtgataacg agcgcgtaac gctcggccgc tattatcaat
    11101 gacaagtttt tctacactgc ttagtgttca tcttattagt atcgcgcttt ctgttgggct
    11161 attaacctta cgtttctggc tacgttatca gaagcatcca caggcatttg ctcgctggac
    11221 gcgcattgtg ccgccggttg tcgatacggt gttattgtta agcggcattg cgttgatggc
    11281 taaagcgcac atcctgccat tttccgggca ggcacagtgg ctgactgaaa agctgtttgg
    11341 agttatcatt tatatcgttt tgggttttat tgcactcgat tatcgtcgta tgcacagtca
    11401 gcaggcgcgc attattgcct tcccgctggc gttggtggtg ctgtacatca tcattaaact
    11461 cgccaccaca aaagtaccgt tactggggta agtcatgaga tcgttagctg atttcgaatt
    11521 taataaagcg ccattgtgcg aaggcatgat cctggcttgc gaagcaatcc gccgcgattt
    11581 tccctcgcaa gatgtttacg acgaactgga gcgtctcgtt agtctggcga aggaagaaat
    11641 cagccagctt ctgcctttag aagagcagct ggaaaaactg atcgcgctgt tttacggcga
    11701 ctggggattt aaagcctcac gcggcgttta tcgtctttcc gatgcattat ggctggacca
    11761 ggtgttaaag aatcgacagg cagtgcggta tcattaggtg cggttttatt atgggtcgcg
    11821 aatcgtctcg atttgccgct gctgccggtg attttcccta cgcagctgat attgcgcatt
    11881 gaatgtccgg atggcgaaat ttggctgatt aatcctttta acggtgaatc gttaagcgaa
    11941 catatgctgg acgtatggtt aaagggaaat atcagcccgt cggcggaact gttttatgaa
    12001 gaccttgatg aagctgataa cattgaggta atccgcaaat tgttggatac actcaaagcc
    12061 tcgttgatgg aagaaaatca gatggagctg gcgttacgca ccagcgaagc tttattacaa
    12121 ttcaacccgg aagatcccta tgaaattcgc gatcgcgggt tgatttatgc gcaactggat
    12181 tgcgaacacg ttgcgttgaa cgatttaagt tatttcgttg aacagtgtcc ggaagacccg
    12241 atcagcgaaa tgatccgtgc gcaaataaat aacatcgcgc ataaacatat tgtgctgcat
    12301 taattaatcg acattttact caagattaag gcgatcctat gaaacaaaaa gtggttagca
    12361 ttggcgacat caacgtagca aatgacctgc cgttcgtact gtttggtggt atgaacgtgc
    12421 tggaatctcg cgatctggcg atgcgcattt gcgagcacta cgtaaccgtg acccagaaac
    12481 tgggtatccc ttacgtgttc aaagcctctt ttgacaaagc caaccgctcc tccatccact
    12541 cttatcgtgg accgggcctg gaagaaggga tgaaaatctt ccaggagttg aagcagactt
    12601 ttggcgtgaa aattatcacc gacgttcacg aaccaagtca ggcacagccc gttgctgatg
    12661 tcgtggatgt gattcagttg ccggcgtttc ttgctcgcca gactgacctg gttgaagcca
    12721 tggcgaaaac cggtgcggta attaacgtca agaaaccaca gtttgtcagc ccgggacaga
    12781 tgggtaatat cgttgataaa ttcaaagaag gcggcaacga aaaagtgatt ctttgcgatc
    12841 gcggtgctaa cttcggctat gacaacctgg ttgtcgatat gctgggcttc agcattatga
    12901 agaaagtgtc tggtaactcg ccggtgattt tcgacgtgac ccacgcactg caatgccgcg
    12961 atccgtttgg cgcagcttcc ggtggtcgtc gtgctcaggt ggctgagctg gcacgagccg
    13021 gtatggcggt aggtctggcg gggctgttta ttgaagcgca tccggatccg gaacatgcga
    13081 aatgtgatgg tccatccgcg ctgccgctgg ctaaactgga accgttcctc aagcagatga
    13141 aagcgattga tgatctggtg aaaggtttcg aagaactgga taccagcaag taatcttttt
    13201 tgcttgaaaa ataaagcctt agcgttctgc gttaagactt ttttcatggg tgccagatac
    13261 aaaaa
//
LOCUS       DAZPJR010000113        13260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPJR010000113 DAZPJR010000000
VERSION     DAZPJR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.114"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHVB7_002991"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHVB7_002991"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1914912.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHVB7_002992"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHVB7_002992"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR1914913.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHVB7_002993"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHVB7_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR1914914.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHVB7_002994"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHVB7_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR1914915.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHVB7_002995"
     CDS             complement(4036..5568)
                     /locus_tag="ACHVB7_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR1914916.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACHGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHVB7_002996"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHVB7_002996"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR1914917.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHVB7_002997"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHVB7_002997"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR1914918.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHVB7_002998"
     CDS             complement(8663..10399)
                     /locus_tag="ACHVB7_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR1914919.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHVB7_002999"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHVB7_002999"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR1914920.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHVB7_003000"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHVB7_003000"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR1914921.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHVB7_003001"
     CDS             complement(12121..12855)
                     /locus_tag="ACHVB7_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR1914922.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="ACHVB7_003002"
     CDS             13056..>13260
                     /locus_tag="ACHVB7_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1914923.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3365 a         3370 c         3489 g         3036 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatggcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc tgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAZPJR010000114        13255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPJR010000114 DAZPJR010000000
VERSION     DAZPJR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13255
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.115"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..843)
                     /gene="adrA"
                     /locus_tag="ACHVB7_003003"
                     /gene_synonym="dgcC"
     CDS             complement(<1..843)
                     /gene="adrA"
                     /locus_tag="ACHVB7_003003"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR1914924.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(945..1265)
                     /gene="psiF"
                     /locus_tag="ACHVB7_003004"
     CDS             complement(945..1265)
                     /gene="psiF"
                     /locus_tag="ACHVB7_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR1914925.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="ACHVB7_003005"
     CDS             complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="ACHVB7_003005"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR1914926.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="ACHVB7_003006"
     CDS             complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="ACHVB7_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR1914927.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3342..3545)
                     /locus_tag="ACHVB7_003007"
     CDS             complement(3342..3545)
                     /locus_tag="ACHVB7_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914928.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3623..4717
                     /gene="ddlA"
                     /locus_tag="ACHVB7_003008"
     CDS             3623..4717
                     /gene="ddlA"
                     /locus_tag="ACHVB7_003008"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1914929.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4741..4953)
                     /locus_tag="ACHVB7_003009"
     CDS             complement(4741..4953)
                     /locus_tag="ACHVB7_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR1914930.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5213..5521
                     /locus_tag="ACHVB7_003010"
     CDS             5213..5521
                     /locus_tag="ACHVB7_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR1914931.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="ACHVB7_003011"
     CDS             complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="ACHVB7_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR1914932.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="ACHVB7_003012"
     CDS             complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="ACHVB7_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR1914933.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8259..9416
                     /gene="ampH"
                     /locus_tag="ACHVB7_003013"
     CDS             8259..9416
                     /gene="ampH"
                     /locus_tag="ACHVB7_003013"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR1914934.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="ACHVB7_003014"
     CDS             complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="ACHVB7_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR1914935.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10128..13010)
                     /locus_tag="ACHVB7_003015"
     CDS             complement(10128..13010)
                     /locus_tag="ACHVB7_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1914936.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITNGGVLDVNTNMNDISSVFAT
                     DTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMNSD
                     GHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYIDD
                     TKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQ
                     DTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGNNA
                     KWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSA
                     TMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMK
                     VDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTE
                     GSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         3366 a         3313 c         3152 g         3424 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
      541 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
      601 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
      661 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac ggggtagtct
      721 gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg
      781 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
      841 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
      901 cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
      961 aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
     1021 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
     1081 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
     1141 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa ggttctctcg
     1201 gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag taatgttatt
     1261 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
     1321 taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact gccgggcgcg
     1381 gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct ggtcggtcag
     1441 tccaacaaca ttggcggcat gcggaccata cgccgcaata cgcaactgac tgccggtatg
     1501 ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc catctttagt
     1561 atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct ggctggcgtg
     1621 ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga attccagcgc
     1681 ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
     1741 gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt tctcattttt
     1801 actcaacaat tcaatggttt tgtcggtcat ctgtgccagg gttggtacac tgtcattacg
     1861 ttgcggatta ggcgtacagg tgactgcggg cttatcgata ttgccgtggt acgttgcttt
     1921 cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca ggggtttttg
     1981 ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact gataaccacg
     2041 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg ccgtttcagc
     2101 aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca gctgttcggt
     2161 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
     2221 cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg tggcatcctg
     2281 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
     2341 cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg ttttgacacc
     2401 ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccagtttt
     2461 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
     2521 aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat cccccatccc
     2581 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
     2641 ggcggtctga tcactcgtta agcggcgagc accgccgggt gcagtaatat cgccctgagc
     2701 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggtaaacag
     2761 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
     2821 tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact ttatgacagc
     2881 tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt acgtaatcgc
     2941 gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg tacagcgccc
     3001 cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga agcattgcag
     3061 tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct tcttcttttt
     3121 gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt ccttatccaa
     3181 agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt attattttta
     3241 tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa acgtttctct
     3301 tatttaaata attacccaca caaaatatag gcttttaatt atcaaccttt attataaata
     3361 aaagttatga aatatattct cttcgttaac ttataattaa gagaaaaaaa ctcaccgcat
     3421 tgccatttaa gccaggaata aattagcttc ccgttcatca tttttgtgat ctcgcgcaca
     3481 tctttgagat aacgcacttt cttcaccagt acgttgcacg ctttgtcagc agaagattgc
     3541 aacaatcttt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat gcactttgtg
     3601 ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt ttggtggtaa
     3661 atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg ccattgataa
     3721 aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa gggcaatggc acgtcagcga
     3781 tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc gcccttcggc
     3841 gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag cttatcgacg cgcaaaacgg
     3901 tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc tgggcgaaga
     3961 tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt ctgatgttct
     4021 ggcttcagca gcctgtatgg ataaagatgt caccaaacgt ctgctacgcg atgccgggct
     4081 gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca gttttgccga
     4141 agtggagtct aaactggggt taccactgtt tgtaaaaccg gctaatcagg gctcttctgt
     4201 tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt gccgtcgatc tggcgttcga
     4261 gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgagatcg aatgcgcagt
     4321 tctgggcaac gacaatccgc aagccagcac ctgtggcgag atcgtactca ccagcgattt
     4381 ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag ttccggcagc
     4441 cattgcacca gagatcaacg ataagatccg ggcgattgcc gttcaggctt atcagacgtt
     4501 gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg aagtggtgat
     4561 caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga agctgtggca
     4621 agccagcggt ctgggttaca ccgatctgat cacacgtttg attgagctgg cgctgaagcg
     4681 tcacgctgcg gataacgcat tgaaagccac aatgtaatat tttaacgccc gataatattc
     4741 ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg ccagccagaa
     4801 actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg cttcaaatcc
     4861 cagtaacccc accacgccat taagaatgaa tataaggcca atggcgaacg catagtagtg
     4921 ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga aaaacaacat
     4981 ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac attttcgtaa ttgttaataa
     5041 atttaactta aggttgccat tttgtaactc acaacgaaga ttcacgcttc gggaatattc
     5101 ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc agacttgcca
     5161 taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt ttatggctga
     5221 tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt caacgcctgg
     5281 caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc aattgctgaa
     5341 cctgttagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg aaacaggtcg
     5401 cccacgggtg gaaattggtt taggtgtcgg caccattttc gggctgatcc cgtttttagt
     5461 aggctgcctc atttttgcag tggtggcgct atggctgcac tggcgacatc gccgccagta
     5521 agatttacgt tgccagatac ggcctaaaac gccttatctg gcatgacatt cctgtttgat
     5581 cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca ggccgtagtc
     5641 aggttgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc gcggggtagt
     5701 gatttacccg ttttcgcttc ggcaagttga taaactttct tgtacagcgc cgtctcctca
     5761 aaagagaagc tgtcaccttt ctctaactga cggcgaattt cgctgtcgct catccccagt
     5821 tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg gttctttact gtcgtaacga
     5881 attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgactgc gttctgaaac
     5941 gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaagcggta tatcggtgcg
     6001 ctatagctgg cgggataatt cagtaaatgg taagtaccaa accacaaccc gccgcggcga
     6061 ctgaagactt cctgacgaac tgtaccgtcc attttccacg gatacccttt ggtatgctgt
     6121 tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc gcaccggatt aagcgaacca
     6181 aacagcgtct gccccattgg taccatgctg atcagatcgt caaaaatcgc gctcaattgc
     6241 ttttctgtac gcaccgaatc taaccgttcg ctataacttt tgccgtttgg cgatttgatt
     6301 tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc gttcagcacg acggtcaatc
     6361 tcttgccagg cgatcttgct taagcccgga acggccggat ctgcctgata atttgactcc
     6421 tgctgcgcca ccgccagcac cgagcagacg ttttccagcg tcggtgccag cccctgactt
     6481 tcaaaggtga tggcgagatc tttcgcccag gcatcgcggt ctttcacgct cgcaggcatc
     6541 ttctgccgca cgacgctcgc tacatctatc gctttttcgc cctttttcaa cggttgcgga
     6601 gcctgactac tacaccccgc taaaaccaga accgccaaca atgacagcga agatagatga
     6661 ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc ctgaattttg
     6721 tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat caactcaacc
     6781 agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc aaaaacgttg
     6841 gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa caattgacgg aaacagcaag
     6901 aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gggcgatgtt gaaatacata
     6961 tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc taaacagctc gcgtaccgta
     7021 ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg gtaggcggcc
     7081 tctactcgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac tgccagccat
     7141 ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg aatgtgcccg
     7201 ataatcggca gctccggcac atgcgcggag agcgttacca gcaccggcag gaaggcgatc
     7261 aacgtcatga tggcgttgat aaaactgacg cccatattct ccagcgttga agcaaaacgc
     7321 atggtgtctt cctgcacacg ctgtgcggca ccttcgatat gacgcagttg ttgccagttc
     7381 gccatgtaat attcgttcat cgccgtacgc cagcggaaca catagtgact gacaaagaag
     7441 ttgttcagta cactgatcac caccgcgatc agcgcaatcc ccagaaagac gcccacttcg
     7501 cggtaaaatt gttcgatagt gactttatgc ggcgaactta gcgcggtttg aatcagatca
     7561 tagaacggtg cataccaggc gttgacggcg accccgactt ccaccaaaaa ccaggtgacg
     7621 aagatgatca gtgcagtacc gagtattgac cagtattgcc aacggtgcgg gctgtagata
     7681 aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa aatcaggaaa
     7741 tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc ggtgatacgc
     7801 gccacccagt ctcccccacc ggcttgccag aagataacgg caatcaatgc ccaaacaaag
     7861 gccgagagaa aaaacgctcc cggctttggg aaaaaagact taaacatatg cactcctgct
     7921 aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccactac aaagcgtcag
     7981 ccaaatgaaa ccgcccattg tgacccatag caaggcgctt agttcgccag cctgaccata
     8041 agaattgttt cgacggattt cacaaaccaa agattgttta cataagcgga ttaacacgcg
     8101 caggatcaca aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg cgccgttacg
     8161 tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag tagtataaat
     8221 acgctcagtt accttcattc aatctatgga caccaccgtt gaaacgtagt ctgctttttt
     8281 ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg atcactgaac
     8341 cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac ggcagcggtg
     8401 caacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgt agttatggcg
     8461 aaacgcgacc tggtaataac gttcgcccgc agctggattc ggtcgtgcgt attgcttccc
     8521 tcaccaaact aatgaccagt gaaatgctgg tgaaattgct cgaccagggg accgtaaagt
     8581 taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtgccgacc tacaatggga
     8641 cgcccattac cctagtgaat ctggcaaccc ataccagcgc cctgccccgt gaacagcccg
     8701 gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg cgagcaacgc tggaaatacc
     8761 tttctacggc gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct aaccttgcgt
     8821 tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc cagttgtttg
     8881 aagagcagat tacccgtcca ttggggatga aagacaccac ctacaccccc tcaccggatc
     8941 agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac acgctggcgg
     9001 caattggcag cggcggcgtc tattccacgc ctggcgatat gatgcgctgg atgcagcagt
     9061 atttgtcatc tgatttttat cagcgcagta accaggcaga tcgcatgcag acgttgattt
     9121 atcagcgcgc gcagtttacg aaagtgattg gcatggatgt ccccggcaaa gcggatgcgc
     9181 ttggtttagg ctgggtatat atggccccga aagagggtcg tccggggatt attcagaaga
     9241 caggcggtgg tggcggtttc attacctata tggcgatgat cccgcagaag aatattggcg
     9301 cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc gatggcatca
     9361 atgatctggt gaccgagcta agcgggaata aaccgttggt tatccccgca tcctgattaa
     9421 tactctgaag gcaaacggtt gatagcgacc agtttgcctt tattcatttc gatatagccg
     9481 cctttacgca aagcagcgag aacttctgcg acgacagaac gcgatatgcg tgtacgttga
     9541 tggatatagt tcatcacacc aatacgtgat cgcaattctt cattccagtc aatcattgat
     9601 aataatgttg cgcggatttg ttcgtaggaa ttatgcccaa tgagctgcac gtcgcgtaat
     9661 tccagaattc tattttgcca agctaaccag taaaaagcgt ctcgccagag ttgattttgt
     9721 tcaataagcg taatggtttg tttggctggt agatgatatc ccgtacaatt tccttctgat
     9781 attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat aatataaggg
     9841 gcctgggtaa taccgataag tacgttttct tctctacgca gagagataac tccctctaaa
     9901 ataacgaaag tatcttcgtt attgacatca ctggaaaata taacttgctt ttcattatta
     9961 aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa ttcctgaaga
    10021 ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa atccttgacg
    10081 ggcattattt agattaaatt accagtattt cttcggagtg aagaatatta ccaggtatat
    10141 ttaacaccca cgttcgcgga ccagtcttga tctacgtcac caccaccgag gtagttagca
    10201 tcggtatagg cgctgaagtt cttggtgaag ctgaactgag tacccagacc aacacgtacc
    10261 gcagaccctt cagtaccgtt atcgatggaa tcaccgttca catcgttatc gttgttagag
    10321 tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag cctgatcttc gctgtaggtg
    10381 aaggtataac ctgcatctac acccagttca taacgcatgc tgtcgtaaga ctgaccgtca
    10441 actttcatgt cgttgctcag ctggtagtca tcaccagact ggaacagacc agaaacgctg
    10501 ccgtaaggag tcacgtaacc agcatcaccc agtttgaagt cgtaaccggc tttcaagccg
    10561 aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag taccgttgct catggttgca
    10621 gacaggtcgt tgttgaagtg agagtagctc aagctaccat caacaaagac gttgttcgcg
    10681 aagtgagcag aagagtagat gtaggcagtc tggctgtctt gatctacctg accagaacgg
    10741 tcattcatgt cacctttagc gaagcctgca gccgcaccga cgatccactt agcgttgtta
    10801 ccgtcaactt tggtatcaac accgaccatg atgccgttaa catcctgatc atagttgatg
    10861 gtgccgttgt cgccgttgaa gttaccaccg aagtagctta cccatgcgcc gccgttatca
    10921 gccaggccat gacgagagtt ggtcagacga gtaccaacgg tgtcttgttc caggttccag
    10981 atattggtgt tcgcagacgg gatgctcagc gccatgttag cgtagtcggt cagctccatc
    11041 tgttgcagaa caacggtgtt accgcgctgt tcagcctgat aggtgtatgc acccaggtca
    11101 gctttgttag cagcagagaa ggtcgcgttg gttttggtat catcgatata gatgagctgc
    11161 ttacctgcgt aatcagcaat cgcaccagcg ccagtggagt tgtcgatacg aactttgtag
    11221 ttaccggttg cagctacaac gttgtctgca gagctgttgt caacttcgtc gccattgtta
    11281 atgtcgttgt taccattaac ggtcaagtga ccatcagagt tcatcgcaat aacaccataa
    11341 ccgtagttag ctttgctggt gtcgttggta cgatcgttga cgaggtctgc attcaggatg
    11401 taatcattac tatgaatgtc aaacacacct gcgtaaacat taccgttgtt atctttaacg
    11461 ttgccactgg tcagttccag agtatcagtt gcaaatacgc tggagatgtc attcatgttg
    11521 gtgtttacat ccagcacacc accgttagta atggtattgg catacagcgg tgcatatgcc
    11581 caagctgaag cagtatcttc accgaagtta acggttgaga aggtatcaat ggtcaggtga
    11641 tcggtgttaa cttcaccgtt aacttccatt gaagaaccac cgttcagagt gatggtgtca
    11701 gaagtcagag aagaatctgc actcacgtta acctgagaac cgctgttgat cgccagggta
    11761 tcgatcaggg aagttttcgt ggtttcccaa tcagaaccgt tgttcagaac aacatcgaat
    11821 acgccgctct ggaatgcgtt agcgttatca acacgattcc agccatcttc agagtaagaa
    11881 gcgtacgggt tcaggctgtt tggtgcaacg tcgtagaagg cttcgtcagc aacaacaacg
    11941 tcataatcaa tattcgctga accaacccat ttggtgttgt tcagagtcag gttcaggtga
    12001 tcgacgttct gatcgtcagt tgcccaaccg ccatgagtgt aagcagtggt agagttgtca
    12061 atcaggtgac ccgtcgcgtc aaagttagcg ttccaggtac tgtcaaatac aacgtcacca
    12121 agcaactgag agttgttgaa cgtaacgttg ttgaccatac gataatcaga agtgtcatca
    12181 tcgatgaagg ctaacgcaac atcatcagca ggagatgcgc cattgccgta gtcacttggt
    12241 tcaacagaat tgccgaaatg accacgatct gattgttcgt cttcagtcca tgaaccagaa
    12301 gttacagtag agtcattaac aacgatgttg ttgtcccact gatgaccttc ggtattgtag
    12361 gtcgcattac cgaggtcgtg ttgaaccaaa gtaatacctg caacatggga gttgttgctg
    12421 atgttgatgt tactttcaac atccagagtc acagcggtac caagaccagc ggttaccaaa
    12481 cgatcataat ctgtcttaga tgttttaccg tctgcatcca gataagtatc ggtgaagtag
    12541 aaggcgtcaa aatcatcgtc aatagtagaa ttagcgatgt tcagagtgaa gatgtcgcca
    12601 tcaacccagt cgtggccagt ataatcgcta ccgttggtcc agacgtagtc gccataacca
    12661 acctggtaag atgtaaccat accatgaaca attgagttgg agatgttcag tgtattaccg
    12721 gtatctgtgc cataatcgtg gttcagataa taagtagaaa ttacaccatt tacagtgtcg
    12781 ccgctgaaag tggtatcaaa gtaaccatca gctgcgtggt catatacata accaccgtaa
    12841 gtcagataat cgtgatcaat tttagtgctg tcaccccaat tgtagatggt ggctgcgtca
    12901 tcgtgatacc cgaaggtgtt ataagtagct gcattagcct gagaggtgat agccagagtg
    12961 caagccaatg ctaattgtga tactacaagt ttctttttcc aggagtgcat ttgtcatccc
    13021 tcctcaggga cgtaataagt tgatccatca attatcaatg catagaaaac atattatgtt
    13081 tcgcgctaac gattatgctg cgttacagac gaatggttca attattcttt cccctaagtc
    13141 cgaatgtgga catatataat ttatggagaa aaacacgcat caaattgata aaaggacgat
    13201 atttcaacat gttgcagata atgttatttt tgatacatac gcgttataaa tatca
//
LOCUS       DAZPJR010000115        13210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPJR010000115 DAZPJR010000000
VERSION     DAZPJR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.116"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..807
                     /locus_tag="ACHVB7_003016"
     CDS             <1..807
                     /locus_tag="ACHVB7_003016"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1914937.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1177..2001
                     /gene="gadX"
                     /locus_tag="ACHVB7_003017"
     CDS             1177..2001
                     /gene="gadX"
                     /locus_tag="ACHVB7_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR1914938.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2369..3097
                     /gene="gadW"
                     /locus_tag="ACHVB7_003018"
     CDS             2369..3097
                     /gene="gadW"
                     /locus_tag="ACHVB7_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR1914939.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3460..6573)
                     /gene="mdtF"
                     /locus_tag="ACHVB7_003019"
     CDS             complement(3460..6573)
                     /gene="mdtF"
                     /locus_tag="ACHVB7_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR1914940.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6598..7707)
                     /gene="mdtE"
                     /locus_tag="ACHVB7_003020"
     CDS             complement(6598..7707)
                     /gene="mdtE"
                     /locus_tag="ACHVB7_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR1914941.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7815..8093
                     /locus_tag="ACHVB7_003021"
     CDS             7815..8093
                     /locus_tag="ACHVB7_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914942.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="ACHVB7_003022"
     CDS             complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="ACHVB7_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR1914943.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="ACHVB7_003023"
     CDS             complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="ACHVB7_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR1914944.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10247..10579
                     /gene="hdeA"
                     /locus_tag="ACHVB7_003024"
     CDS             10247..10579
                     /gene="hdeA"
                     /locus_tag="ACHVB7_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR1914945.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10695..11021
                     /gene="hdeB"
                     /locus_tag="ACHVB7_003025"
     CDS             10695..11021
                     /gene="hdeB"
                     /locus_tag="ACHVB7_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR1914946.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11085..11732
                     /locus_tag="ACHVB7_003026"
     CDS             11085..11732
                     /locus_tag="ACHVB7_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR1914947.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11774..12304)
                     /locus_tag="ACHVB7_003027"
     CDS             complement(11774..12304)
                     /locus_tag="ACHVB7_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR1914948.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12460..13026)
                     /gene="slp"
                     /locus_tag="ACHVB7_003028"
     CDS             complement(12460..13026)
                     /gene="slp"
                     /locus_tag="ACHVB7_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR1914949.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3599 a         2982 c         3030 g         3599 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca cacctgataa cataacgttg taaaaaccga atgcccagcc
      841 tttaaaaaaa cagctgggca ttcggttgct tattaatcgc aataatatat tggctgtcta
      901 ttcatcgtgt tgataagata tatgttatat gaatgtttat taaatgtagc gattgccttt
      961 aatgtcacga aaagggatta tttgcttgct attaatttca ctgcgttctt aaacacattt
     1021 ttgtattata aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc
     1081 cgcttcgttt aaatttattt atcaatcaat ttgacttaag agggcggcgt gctacattaa
     1141 ttaacagtaa tatgtttatg taatattaag tcaactatgc aatcattaca tgggaattgt
     1201 ctaattgcgt acgcaagaca taaatatatt ctcaccatgg ttaatggtga atatcgctat
     1261 tttaatggcg gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga taagtgtgtt
     1321 gaaaattttg tattagtatc aagggatacg ctttcattat ttctgccgat gctcaaggag
     1381 gaggcattaa atcttcatgc acataaaaaa atttcttcat tactcgttca tcactgtagc
     1441 agagatattc ctgtttttca ggaagttgcg caactatcgc agaataagaa tcttcgctat
     1501 gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt tatctgtttt tcttgaggat
     1561 gagcacttta taccgctgct tttgaacgtt ttgcaaccga acatgcgaac acgagtttgt
     1621 acggttatca ataataatat cgcccatgag tggacactag cccgaatcgc cagcgagctg
     1681 ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag aagagacatc atattcacag
     1741 ttgcttactg agtgtagaat gcaacgtgct ttgcaactta ttgttataca tggcttttca
     1801 attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat ttacgtcttt
     1861 cgaaattatt atgggatgac gcccacagag tatcaggagc gatcggcgca gggattgccg
     1921 aaccgtgact cggcggcaag tattgttgcg caaggaaatt tttacggcac tgaccgttct
     1981 gcggaaggaa taagattata gaattttact cagacataaa aaaaacccgg cataggggac
     2041 cgggaagagg atagtctgcc gtctccagac taataaaccg ttataacact ccctgttggc
     2101 acgggaaact ttgtgctctc agtaagttaa atataacttt tactggaaat aagatcagcc
     2161 atttttttat aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta
     2221 ttatcaatta actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg
     2281 gcagtttttt cgctaagaaa cagtctggca acatttcatt agtatactga aattgaaata
     2341 atcgcagtat gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg
     2401 tcgttcattc gatatttatc atgaacagca taaaatatcg ctgcataacg agagtatcgt
     2461 gctgctggag aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact
     2521 ggatatcgat gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa
     2581 tttagggtta catagcaaag accgtctatt aattaaccag tcacccccca tgccgctggt
     2641 gacggcgatt tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat
     2701 gctctacctt tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt
     2761 caatagcatc agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa
     2821 acgttggtat ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcataaaaaa
     2881 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat
     2941 ggccagacga ttactcgagt tacgtcaaat tcctctgcat actattgcag aaaaatgtgg
     3001 ctatagcagt acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca
     3061 tcagtttgcg caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg
     3121 tcaggatctc acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg
     3181 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     3241 catgtttggg attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt
     3301 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     3361 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     3421 ggctgaacct tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     3481 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     3541 ggcaaacatc ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc
     3601 atgactgata accagcggca gcacgccgag aataaaggcg agagaggtca tcaggattgg
     3661 gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg
     3721 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     3781 ggtcagcaaa ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag
     3841 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     3901 ctcatagagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg
     3961 agcctggttt gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc
     4021 tgccggaagt ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc
     4081 ggccgccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg
     4141 tgaaccatag gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc
     4201 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     4261 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     4321 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc
     4381 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt
     4441 gacctggtca ggcgattgcg ctgccagtga taacagctcg tttcgtgcct gggttagttt
     4501 ttcgtgcccc aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     4561 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc
     4621 aatcattgcc cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca
     4681 cggcttgaga ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc
     4741 gccaacggta aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac
     4801 ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt
     4861 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac
     4921 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     4981 cgagcgggtg ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     5041 gaacagggcg ttaggtttgt gaccgccttc cggcgcggct ttcagaatgg tggcgcacag
     5101 ggcaggggtc aggctcattg ccacaaatac tgaaagcagc atggaggaga tcagcgtgat
     5161 ggagaactgg cggtagatct ccccggttgc gccgctcata aaggccatcg gcataaacac
     5221 tgcggaaaga acaacagcaa taccgaccag cgcacgttgg atctgcccca tcgatttatg
     5281 cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     5341 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     5401 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     5461 ggcaatcgtc gggatgattg tggcacggaa attctgcaaa aacagataca tgaccaggaa
     5521 gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa
     5581 cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg
     5641 gttcagctcc tctttgactg cccgcgaggt atccagggcg tttgctccgg tagccagttt
     5701 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     5761 tccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     5821 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     5881 gtttagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     5941 gttttgcacc ttaatctggg aaataacatc ggaaggtacc agattgtatt tattgagttt
     6001 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     6061 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta
     6121 ctggttgagg ctgccgttat cagaaataaa cgccgctacc atcagaatat tactgctcga
     6181 cttatcaacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     6241 cagtttattt tgcacctgaa cctgtgcgat atcaggagat gtcccagtct cgaaggtcag
     6301 agtgatagag gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag
     6361 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     6421 acctggatag gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     6481 cggtaagttc atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc
     6541 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     6601 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     6661 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     6721 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacaacatc
     6781 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgagtga cgccttcctg
     6841 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga cgtacatgcc
     6901 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     6961 ttcatccacc gtcgggtcgg agaatttcag tgtgccggtc tggctgtagc gtttaccatt
     7021 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     7081 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     7141 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     7201 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     7261 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcag
     7321 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag ttggtcttca gcaacgatgc
     7381 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     7441 tttggcggag tttagctcgg cctgtaaagg tgcaggatca atctgataca gcgaatcgcc
     7501 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataataccgc ccacctgggg
     7561 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     7621 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     7681 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagctt
     7741 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttt tttaaattgc
     7801 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     7861 aggtgggata caggcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     7921 ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     7981 acctggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt aatgaaatca
     8041 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     8101 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     8161 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     8221 ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     8281 aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     8341 ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     8401 ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat cgcttcttca
     8461 tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt gcctactttt
     8521 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     8581 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     8641 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     8701 atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt aacactaatc
     8761 aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag attcctggtt
     8821 gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg cccaagaata
     8881 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     8941 atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc cagctttctt
     9001 ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt tttatatagc
     9061 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     9121 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     9181 cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata atgtttgaaa
     9241 tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg aaataatcac
     9301 tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg tagcggagag
     9361 agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa ccgggataat
     9421 tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct aaatatcagt
     9481 tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc accaaggaat
     9541 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct gcctttcatt
     9601 gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa caaaccggca
     9661 ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa atagccgatc
     9721 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     9781 ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     9841 gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc gacgataaac
     9901 agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     9961 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
    10021 atcccctgct atcaatctat gccaaaaacg cgtctaagaa tgtagtcgat ttaataaaaa
    10081 tttcctaatt acagtatctg atgcatctgt aactcattgt attgaaataa aaatatctga
    10141 ttttgatatt ttatatcaac atgacatata cagaaaacca ggttataacc tcagtgtcga
    10201 aattgattcg tgacggctct ttcactttat agttgaggat attacgatga aaaaagtatt
    10261 aggcgttatt cttggtggtc tgcttcttct gccagttgtg agcaatgcag cggatgcgca
    10321 aaaagcagct gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt
    10381 ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa
    10441 accagaagat gcggttttag atgttcaggg tattgcaacc gtaaccccag ctatcgttca
    10501 ggcttgtact caggataaac aagccaactt taaagataaa gttaaaggcg aatgggacaa
    10561 aattaagaaa gatatgtaat tccgggaatg cgttacatcg tacttccttg catattgaac
    10621 aggccggaat atcttcttta aaagcagcta ttcctcctgt tcatatataa tctctatatt
    10681 gaatgggtta caaaatgaat atttcatctc tccgtaaagc gtttattttt atgggcgctg
    10741 tagcggcttt gtcactggtg aacgcacaat ctgcgctggc agccaatgaa tccgctaaag
    10801 atatgacctg ccaggaattt attgatctga atccaaaagc aatgaccccg gttgcatggt
    10861 ggatgctgca tgaagaaaca gtatataaag gtggcgatac cgttacttta aatgaaaccg
    10921 atctcactca aattcctaaa gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt
    10981 ataccttcaa aaatcaagca tctaatgact tgccgaatta atgaggtaca agtaaaaagg
    11041 agtagcaagt tgagccatct tgctgctcct ttttgcattt ttatatgaca gcagaattta
    11101 ttatacgtct tatactcgcg gcaattgcct gtggcgctat tggcatggaa aggcaaatgc
    11161 gcggcaaagg agcagggtta cgcacacatg tattaattgg catgggaagc gccctgttta
    11221 tgattgtttc gaaatatggt tttgctgacg tgctgtcttt agatcacgtc ggactcgacc
    11281 ccagccgtat tgctgctcag gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc
    11341 tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc ggatatctgg gtgaccgccg
    11401 ccataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
    11461 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
    11521 atttacagct gacattagtg aacggtaata tggtatcaat gctcgactgg tttaagcagc
    11581 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
    11641 ttgatatcca gcttcatgcc acaacgtcga tagaagacct gttgcgatta ttaaaaggta
    11701 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
    11761 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
    11821 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
    11881 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
    11941 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
    12001 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
    12061 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
    12121 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
    12181 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
    12241 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
    12301 acataaccga ctcctggtgc ggacattaac tttataatca atttgccaaa ggtaattaac
    12361 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
    12421 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
    12481 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
    12541 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
    12601 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagccagg
    12661 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactgggtc
    12721 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggttt
    12781 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
    12841 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
    12901 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
    12961 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac cttttgtcat
    13021 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
    13081 ttcatctgat tctgaggcga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
    13141 ccttttgttt agttcaattc cattgatttt ggggcgtggc atgagtatcc atcgccccat
    13201 aatataatcc
//
LOCUS       DAZPJR010000116        13015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPJR010000116 DAZPJR010000000
VERSION     DAZPJR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.117"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            43..342
                     /locus_tag="ACHVB7_003029"
     CDS             43..342
                     /locus_tag="ACHVB7_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914950.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(389..1276)
                     /gene="rarD"
                     /locus_tag="ACHVB7_003030"
     CDS             complement(389..1276)
                     /gene="rarD"
                     /locus_tag="ACHVB7_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR1914951.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1328..1795)
                     /gene="yigI"
                     /locus_tag="ACHVB7_003031"
     CDS             complement(1328..1795)
                     /gene="yigI"
                     /locus_tag="ACHVB7_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR1914952.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1960..2829
                     /gene="pldA"
                     /locus_tag="ACHVB7_003032"
     CDS             1960..2829
                     /gene="pldA"
                     /locus_tag="ACHVB7_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR1914953.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2962..4791
                     /gene="recQ"
                     /locus_tag="ACHVB7_003033"
     CDS             2962..4791
                     /gene="recQ"
                     /locus_tag="ACHVB7_003033"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR1914954.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4855..5472
                     /gene="rhtC"
                     /locus_tag="ACHVB7_003034"
     CDS             4855..5472
                     /gene="rhtC"
                     /locus_tag="ACHVB7_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR1914955.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5534..6154)
                     /gene="rhtB"
                     /locus_tag="ACHVB7_003035"
     CDS             complement(5534..6154)
                     /gene="rhtB"
                     /locus_tag="ACHVB7_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR1914956.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6265..7287
                     /gene="pldB"
                     /locus_tag="ACHVB7_003036"
     CDS             6265..7287
                     /gene="pldB"
                     /locus_tag="ACHVB7_003036"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR1914957.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7295..8095
                     /gene="yigL"
                     /locus_tag="ACHVB7_003037"
     CDS             7295..8095
                     /gene="yigL"
                     /locus_tag="ACHVB7_003037"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR1914958.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8171..9070
                     /gene="bioP"
                     /locus_tag="ACHVB7_003038"
     CDS             8171..9070
                     /gene="bioP"
                     /locus_tag="ACHVB7_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR1914959.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8958..9911)
                     /gene="metR"
                     /locus_tag="ACHVB7_003039"
     CDS             complement(8958..9911)
                     /gene="metR"
                     /locus_tag="ACHVB7_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR1914960.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10147..12408
                     /gene="metE"
                     /locus_tag="ACHVB7_003040"
     CDS             10147..12408
                     /gene="metE"
                     /locus_tag="ACHVB7_003040"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR1914961.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12530..12652)
                     /locus_tag="ACHVB7_003041"
     CDS             complement(12530..12652)
                     /locus_tag="ACHVB7_003041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914962.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12693..12938)
                     /locus_tag="ACHVB7_003042"
     CDS             complement(12693..12938)
                     /locus_tag="ACHVB7_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR1914963.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3072 a         3248 c         3475 g         3220 t
ORIGIN      
        1 atttttattc tataaacaat atcttgggtg aatggattct atatgggaaa tggtaacttt
       61 tggcagttgt tcttgggtaa agaaccttac agcaaaaaat ctgattttgt taaggcatgg
      121 ttattgtggt gggctattac catccttgtt ctatttattt taagcctgac tacgatatta
      181 aatttaagaa gtataggtat agggttaagt tttgggtttt gtcaaatcct tggtagatat
      241 acgatgtctc taggatggag taagtactgg ggagttttgg gggtatttcc tgtaacaaat
      301 attgttcttt ttcttatctt aatttttttc aaaaaaagat aaataagtca gtgggtaggc
      361 tctttaaacg gcctacccat gttaattctt aactttttct acgctgcgta taaatcgcat
      421 ccatcacaaa aattgccagc gccacccaaa taaaggcgaa agtcaccatc ttatcggcac
      481 ccggtttttc accataaaac gtcacagcca gcaggaacat cagcgtcggg ccaatgtact
      541 ggaaaaagcc taacgttgag agacgcaagc gcgtggcagc ggcggtaaaa cacaacagcg
      601 gtacggtagt gacaataccg gcggcgatca gcagtaaatt cagcgacatc gggttttgcc
      661 ccatatggct ggttgagctg tcggcaatag caaacaggta aattgccgcc acgggcagca
      721 gccacatggt ttcgattaac atgccggttt gcgcttcaac ggcaatcttc ttgcgtacca
      781 gaccgtagaa ggcaaaacta aatgccagtc ccagcgcgat aataggtagc gaaccaaaag
      841 tccacagctg gactaacacg ccacatatcg ccagaatcac cgccagccat tgcatccggc
      901 ggaatcgctc gccgaggaaa atcatcccca gcacaatgtt caccagcggg ttaataaagt
      961 aaccaaggct cgcttccagc atatggtgat tgttcaccgc ccagataaac agtagccagt
     1021 tgccaccaat cagcacggca gagactgcca gcataaaaat tttctgtggc gtctgaatca
     1081 gcgtttttaa ataggaccac tggcggcaaa tgctcatcag caccaccata aagaaaaacg
     1141 accagatcac gcgatgcgtc aggatttcat cggcgggcac gtagtaaatc aacttgaagt
     1201 acgctggcgc tataccccaa ataaaataag cggcaagagc gagtaatacg ccctgccgcg
     1261 tttgttttgc atccatcggg aatactcatt tttaattggt aacagcagtt tacctgcttt
     1321 tatgtcttca acctaccata taggtggcgg tggcactggc aatataaagc tgttcttcat
     1381 tgtgtaattc aacgcgggcg acggcgactt tattgcctgc acgcaacagg ctactggtag
     1441 cagtaaaacg ctcgcccctg cctgggcgca gataatcaac gcgaagatca atggtcccca
     1501 tccgcgatag ccgctggcgt agttcatctt cactgatggt ttcgtggcgg gttaaggtac
     1561 ttcccacgca caccagaccg gcggcgacat ccagcgccga cgcaatgacc ccgccgtgca
     1621 aaatgctttg cgcccagttg cccaccatca ttggctgatt tttaaaggcc agctgtgcga
     1681 actctttttc gtaacgctcc agttccatcc ccaatgcgcg gttaaatggc atgtgataaa
     1741 caaacatctc acccactaat ttcagggctt gttcagcggt cagtacggca gacatatcat
     1801 ccttacactt cattggttaa tgaaatgttg attttatgct tctttgttgt tggtttctac
     1861 tttaggaagg gataactaac ggctatggag ttaagtatgt aaaatagccc gcagaaaaat
     1921 attcacctta tcaataattc gttacggaga acacgaccga tgcggactct gcagggctgg
     1981 ttgttgccgg tgtttatgtt gcctatggca gtatatgcac aagaggcaac ggtgaaagag
     2041 gtgcatgacg cgccagcggt gcgtggcagt attatcgcca atatgctgca ggagcatgac
     2101 aatccgttca cgctctatcc ttatgacacc aactacctca tttacaccca aaccagcgat
     2161 ctgaataaag aagcgattgc cagttacgac tgggcggaaa atgcgcgtaa ggatgaagta
     2221 aagtttcagt tgagcctggc atttccgctg tggcgtggga ttttaggccc gaactcggtg
     2281 ttgggtgcgt cttatacgca aaaatcctgg tggcaactgt ccaatagcga agagtcttca
     2341 ccgtttcgtg aaaccaacta cgaaccgcaa ttgttcctcg gttttgccac cgattactgt
     2401 tttgcaggtt ggacgctgcg cgatgtggag atggggtata accacgactc taacgggcgt
     2461 tccgacccga cctcccgcag ctggaaccgc ctttatactc gcctgatggc agaaaacggt
     2521 aactggctgg tagaagtgaa gccgtggtat gtggtgggta atactgacga taacccggat
     2581 atcaccaaat atatgggtta ctaccagctt aaaatcggct atcacctcgg tgatgcggtg
     2641 ttgagtgcga aaggacagta caactggaat accggttacg gcggcgcgga gttaggctta
     2701 agttacccga tcaccaaaca tgtgcgcctt tatactcagg tttacagcgg ctatggcgaa
     2761 tcgctcatcg actataactt caaccagacc cgtgtcggtg taggagttat gctaaacgac
     2821 ttgttttgat gaacggttga gtggttggca aatctggaac ccagcatcca ggattaccct
     2881 ctcagagact aaaagcattg cagtttctcg cgcaggcgct gaaaatagcg cctgttttta
     2941 tttcaggcaa tcggggggaa tgtggcgcag gcggaagtgt tgaatctgga gtccggagct
     3001 aaacaggttt tacaagaaac ctttggctac caacagtttc gccccggcca ggaagaaatt
     3061 atcgacactg tgctttccgg tcgcgattgc ctggtcgtca tgcccaccgg tggcggaaaa
     3121 tccctttgct atcaaattcc tgccttattg ctaaacggcc ttaccgtggt tgtttcaccg
     3181 ctgatttcgt tgatgaaaga tcaggtggat caactgcaag ccaacggcgt ggcggcggcg
     3241 tgccttaact cgacgcaaac ccgcgagcag caacttgaag tgatgacagg ctgccgcacc
     3301 gggcaaattc gtctgcttta tatcgccccg gaacgcctga tgctggataa ctttcttgag
     3361 catctggcgc actggaatcc ggtgttatta gccgttgatg aagcgcactg tatctcccaa
     3421 tggggccacg atttccgccc ggaatatgcc gcgcttggtc agttacgcca gcgtttcccg
     3481 accctgccgt ttatggcgct gactgccaca gccgacgaca ccacgcgcca ggatatcgtg
     3541 cgcttgctgg ggctgaacga tccgctgatt caaatcagca gttttgaccg tccgaatatt
     3601 cgctacatgc tgatggagaa attcaaaccg ctcgatcagt tgatgcgcta cgtgcaggaa
     3661 cagcgcggta agtccggcat tatctactgc aacagccggg cgaaagtaga agacaccgct
     3721 gcgcgcctgc aaagcaaggg tattagcgcg gcggcctatc atgccgggct ggaaaataat
     3781 gtccgcgccg acgtgcagga gaaattccag cgcgatgacc tgcaaattgt ggtggcgacg
     3841 gtggcgttcg gcatgggcat caataaacca aacgttcgct tcgtggtcca ctttgatatt
     3901 ccgcgcaata tcgaatccta ttatcaggaa accggtcgcg ccgggcgtga tggcctgcct
     3961 gcggaagcga tgctgtttta cgatccggct gatatggcgt ggctgcgccg ttgtctggaa
     4021 gagaagccgc aggggcagtt gcaggatatt gagcgccaca aactcaatgc gatgggcgcg
     4081 tttgccgaag cgcaaacttg ccgtcgtctg gtattgctga actattttgg cgaagggcgt
     4141 caggagccgt gcgggaactg cgatatctgc ctcgatccgc cgaaacagta cgacggttca
     4201 accgatgctc agattgccct ttccaccatt ggtcgtgtga atcagcggtt tgggatgggt
     4261 tatgtggtgg aagtgattcg tggtgctaat aaccagcgta tccgcgacta tggtcatgac
     4321 aaactgaaag tctatggcat gggccgtgat aaaagccatg aacattgggt gagcgtgatc
     4381 cgccagctga ttcacctcgg cctggtgacg caaaatattg cccagcattc tgccctacaa
     4441 ctgacagagg ccgcgcgccc ggtgctgcgc ggcgaatcct ctttgcaact tgccgtgccg
     4501 cgtatcgtgg cgctcaaacc gaaagcgatg cagaaatcgt tcggcggcaa ctatgatcgt
     4561 aaactgttcg ccaaattacg caaactgcgt aaatcgattg ccgatgaaag taatgtcccg
     4621 ccgtacgtgg tgtttaacga cgcaaccttg attgagatgg ctgaacagat gccgatcacc
     4681 gccagcgaaa tgctcagcgt taacggcgtt gggatgcgca agctggaacg ctttggtaaa
     4741 ccgtttatgg cgctgattcg tgcgcatgtc gatggcgacg acgaagagta gtcagcagca
     4801 taaaaaagtg ccaggatgaa gactccgtaa acgtttcccc cgcgagtcaa atgtatgttg
     4861 atgttatttc tcaccgtcgc catggtgcac attgtggcgc ttatgagccc cggtcccgat
     4921 ttcttttttg tctctcagac cgctgtcagt cgttcccgta aagaagcgat gatgggcgtg
     4981 ctgggcatta cctgcggtgt aatggtttgg gctgggattg cgttgcttgg cctgcatttg
     5041 attatcgaaa aaatggcctg gctgcatacg cttatggttg gcggtggcct gtatctctgc
     5101 tggatgggtt accagatgct gcgcggcgcg ctgaaaaaag aggcggtttc tgcacctgcg
     5161 ccacaggtcg agctggcgaa aagtgggcgc agtttcctga aaggtttact gaccaatctc
     5221 gctaatccga aagcgattat ctactttggc tcggtgttct cattgtttgt cggtgataac
     5281 gttggcacta ccgcgcgctg gggcattttt gcgctgatca ttgtcgaaac gctggcgtgg
     5341 tttatcgtcg ttgccagcct gtttgccctg ccgcaaatgc gccgtggtta tcaacgtctg
     5401 gcgaagtgga ttgatggttt tgccggggcg ttatttgccg gatttggcat tcatttgatt
     5461 atttcgcggt gatgccagac gcgtcttcag agtaagtcgg ataaggcgtt tacgccgcat
     5521 ccgacattat ttttcacgca tgcctcgccg atgctaacag cgctcccacc agcataaaca
     5581 acgagccgaa aatcttattc agcgccttca tctgctttgg tcctttaatc catagagcaa
     5641 tccgttgagc aagggtggcg taaccgatca tcacaataat atcgaccaca atagtggtga
     5701 cgccgagcac gatatactgc atcagttgcg gctgttgcgg catgatgaat tgcggaaata
     5761 gcgccgccag aaacacaata cttttgggat tggtgagatt cacaaaaact gcgcgctgga
     5821 acaaatgtcg acgcgattga gtagaggcca gcgatttaag gtcaattgca ccagcggcgc
     5881 gccactgctg gattcccagc caaatcaagt aagccgcgcc tgcccacttc aacacttcaa
     5941 acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac gccaaccagc acaatatgaa
     6001 tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc gccgcgataa ccgtggttga
     6061 gcgaggtggt catagtgttg attgcaccag agcctggcga caggcttaaa atgatcgatg
     6121 tcagcaggta ggcaaaccac cattctaagg tcatgatgaa ctcccggtgt gtctattttt
     6181 gtgccacaat acgctactgt cgcagcgttg tgtcaggcac gctaaaaaaa acgattttac
     6241 gtggtttaag aggcagatta cccgatgttt cagcagcaaa aagactggga aacaagagaa
     6301 aacgcgtttg ctgcttttac catgggaccg ctgactgatt tctggcgtca gcgtgatgaa
     6361 gcagagttta ctggtgtgga tgacattccg gtgcgctttg tccgttttcg cgcacagcac
     6421 catgaccggg tggtagtcat ctgcccgggg cgtattgaga gctacgtaaa atatgcggaa
     6481 ctggcctatg acctgttcca tttggggttt gatgtcttaa tcatcgacca tcgcgggcag
     6541 ggacgttccg gtcgcctgtt agccgatccg catctcgggc atgttaatcg ctttaatgat
     6601 tatgttgatg atctggcggc attctggcag caggaggttc agcccggtcc gtggcgtaaa
     6661 cgctatatac tggcacattc gatgggcggt gcgatctcca cattatttct gcaacgccat
     6721 ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt ttgggatcgt gattcgtatg
     6781 ccgtcattta tggcacggca gatcctcaac tgggccgaag cgcatccacg tttccgtgat
     6841 ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt ttgctatcaa cgtactgacc
     6901 cacagcagac agcgatatcg acgtaactta cgcttctatg ctgatgaccc aacgattcgc
     6961 gtcggtgggc cgacctacca ttgggtacgc gaaagtattc tggctggcga acaggtgtta
     7021 gccggtgcgg gtgatgacgc cacgccaacg cttctcttgc aggctgaaga ggaacgcgtg
     7081 gtggataacc gcatgcatga ccgtttttgt gaactccgca ccgccgcggg ccatcctgtc
     7141 gaaggaggac gaccgttggt aattaaaggt gcttaccatg agatcctttt tgaaaaggac
     7201 gcaatgcgct cagtcgcgct ccacgccatt gttgattttt tcaacaggca taactcaccc
     7261 agcggaaacc gctctacaga ggtttaaatt tcttatgtac caggttgttg cgtctgattt
     7321 agatggcacg ttactttctc ccgaccatac gttatcccct tacgccaaag aaaccctgaa
     7381 gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc ggtcgtcacc acgttgatgt
     7441 ggggcaaatt cgcgataatc tggagatcaa gtcgtacatg atcacctcca acggcgcgcg
     7501 cgttcacgat ctggatggca atctgatttt tgctcataac ctggatcgcg acattgccag
     7561 cgatctgttt ggcgtagtca acgacaatct ggacatcatt actaacgttt atcgcgacga
     7621 cgaatggttt atgaatcgcc atcgcccgga agagatgcgc ttttttaaag aagcggtgtt
     7681 caaatatgcg ctgtatgagc ctggattact ggagccggaa ggcgtcagca aagtgttctt
     7741 cacctgcgat tcccatgaac aactgctgcc gctggagcag gcgattaacg ctcgttgggg
     7801 cgatcgcgtc aacgttagtt tctctacctt aacctgtctg gaagtgatgg cgggcggcgt
     7861 ttcaaaaggc catgcgctgg aagcggtggc gaagaaactg ggctacagcc tgaaggattg
     7921 tattgcgttt ggtgacggga tgaacgatgc cgaaatgctg tcgatggcgg ggaaaggctg
     7981 cattatgggc agtgcgcacc agcgtctgaa agaccttcat cccgagctgg aagtgattgg
     8041 tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa ctctatttat cgtaatcgtt
     8101 ctttatttgg tcagttgtca acctgatact tcgctacaat ggatactcgt taatcaaaga
     8161 gttttccatt gtggcgctac ttatcatcac cacgattctg tgggccttct cctttagctt
     8221 ttatggcgag taccttgcgg ggcacgtcga tagctatttt gcggtgctgg tgcgcgttgg
     8281 cctggcggca ctcgtttttc tgccgtttct gcgtacccgt ggcaatagcc tgaaaacggt
     8341 cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg atgtatatgc tgagtttccg
     8401 cgcttatctc tacctgacgg tctccgaatt gttgctgttc accgtactga cgccgctcta
     8461 catcacgctg atttatgaca tcatgagtaa gcgccgtctg cgctggggct atgcttttag
     8521 cgccttgctg gcggtgattg gtgccgggat tattcgctat gatcaggtta ccgaccattt
     8581 ctggactggc ttgttgctgg tgcaactctc caatatcact tttgccatcg gcatggtggg
     8641 ttacaaacgc ctgatggaaa ctcgcccgat gccgcagcat aacgcctttg cgtggttcta
     8701 tcttggcgcg ttcctggtgg cggtaattgc atggttcttg ctgggaaatg cgcagaaaat
     8761 gccgcaaacc acgttgcaat ggggcattct ggtgtttctt ggcgtggtgg cttccgggat
     8821 tggctacttt atgtggaact acggcgcgac gcaggtggac gccggaacgc tgggcattat
     8881 gaataatatg cacgttccgg cagggctgct ggtaaacctg gctatctggc accaacagcc
     8941 gcactggcca acgttcatta caggcgcgct ggtgatcctg gcctcactgt gggtgcatcg
     9001 taagtgggtc gctccgcgct cttcacaaac ggcagatgat cgcaggcgtg attgcgcgct
     9061 gagcgaataa atgcttctgt aatcggctga cgctgctcgc catcgcgcac ggcggcgtac
     9121 agtcggctcc acaagccttc gcccagggtt ttggtcacca ccagaccttg gcgctcaaaa
     9181 ctctctacta cccaatgcgg tagcgcggca atacccatcc gcgcggcaac catctgaatc
     9241 aacaataagg tgttatcgac gcttttcagt gacgggctga cgcctgccgg ctgaagaaaa
     9301 tgccgccaga catccagtcg actacgctgc accggataaa tcaacagcgt ctcgctggcg
     9361 agatcttccg gtgtaattcg cgttttcgcc gccagtggat ggtcaggtgc taacaccaga
     9421 cgcacttcat agtcgaacat cggcgaataa tgcaggccac tgcgcggcag gatatcggag
     9481 gtcatcacca gatccagctc gccctgttgc aacgcaggct gcgggtcaaa cgtcacgccc
     9541 gatttaaaat ccatctctac ctgcggccag ttcttatgga aattttctaa cgcgggcgtc
     9601 agccactgaa tacagctatg gcactcaatg gcaatgcgca gacgcgtctg ctgcggttca
     9661 ttgcaggctt gcagtgcctg gctgatttgc ggcagcacct ggtttgccag ttgcaacagg
     9721 atttctccct gcggtgtaaa gcgtagcggc tggctcttac gcacaaatag ccggaagcca
     9781 aggcgttgtt ccagatcgct aaactggtga gacagggcgg attgcgtctg atgcaacgtc
     9841 gccgcagcgg ctgcgagaga gccgcagttc cgcaacgctt gtagcgtttt caggtgtttt
     9901 acttcgatca tgaaagtcct tcacttcggc atgaataatt tgcgcttgag gaatatacag
     9961 taaccgccaa ttatggatgt gtaaacatct ggacggctaa aatccttcgt cttttaaatt
    10021 tatggtgcgt tggctgcgtt tctccacccc ggtcacttac ttcagtaagc tcccggggat
    10081 gaataaactt gccgccttcc ctaaattcaa aatccatagg atttacatat aattagagga
    10141 agaaaaatga caatattgaa tcacaccctc ggtttccctc gcgttggcct gcgtcgcgag
    10201 ctgaaaaaag cgcaagaaag ttattgggcg gggaactcca cgcgtgaaga actgctgaca
    10261 gtagggcgtg aactgcgtgc tcgtcactgg gatcaacaaa agcaagcggg tatcgacctg
    10321 ctgccggtgg gcgattttgc ctggtacgat catgtactga ccaccagttt gctgctgggt
    10381 aatgttccgc cacgtcatca gaacaaagac ggttcggtag atatcgacac cctgttccgt
    10441 attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg cagcggaaat gaccaaatgg
    10501 tttaacacca actatcacta catggtgccg gagttcgtta aaggccaaca gttcaaactg
    10561 acctggacgc agctgctgga ggaagtggac gaggcgctgg cgctgggcca caacgttaag
    10621 cctgtgctgc tggggccggt tacttacctg tggctgggga aagtgaaagg tgaacagttt
    10681 gaccgcctta gcctgctgaa cgacattctg ccggtttatc agcaagtgct ggcagaactg
    10741 gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg cgctggtact ggaactacca
    10801 caggcgtggc tggacgcata caaacccgct tacgacgcgc tccagggaca ggtgaaactg
    10861 ctgctgacca cctattttga aggcgtaacg ccaaatctcg acacgattac tgcgctgcct
    10921 gttcagggtc tgcatgttga tctcgtacat ggtaaagatg acgttgctga actgcacaag
    10981 cgcctgcctt ctgactggtt gctgtctgcg ggtctgatca atggtcgtaa cgtctggcgc
    11041 gccgatctta ccgagaaata tgcgcaaatt aaggacattg tcggcaaacg tgatttgtgg
    11101 gtggcatctt cctgctcgtt gctgcacagc cccatcgacc tgagcgtgga aacgcgtctt
    11161 gatgcagaag tgaaaagctg gtttgccttc gccctacaaa aatgccatga actagcactg
    11221 ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg cagagtggag cgccccaatt
    11281 caggcgcgtc gtcactctac tcgtgtacat aatccggcgg tagaaaagcg tctggcggcg
    11341 atcaccgccc aggacagcca gcgtgcgaat gtctatgaag tgcgtgctga agcccagcgt
    11401 gcacgtttta aactgccagc gtggccgacc accacgattg gttccttccc gcaaaccacg
    11461 gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc tcgacgccaa taactaccgc
    11521 acgggcattg cggaacatat caggcaggcc attgttgaac aggaacgttt gggactggat
    11581 gtgctggtac atggcgaggc cgagcgtaat gacatggtgg aatactttgg cgagcacctc
    11641 gacgggtttg tctttacgca aaacggttgg gtacagagct acggttcccg ctgcgtgaag
    11701 ccaccgattg ttattggtga cgttagtcgc ccggcaccga ttaccgtgga gtgggcgaag
    11761 tatgcgcaat ctctgactga taaaccggtg aaagggatgc tgactggccc ggtgactatt
    11821 ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa ccatcgccaa acagattgcg
    11881 ctggcgctgc gtgatgaagt ggccgatctg gaagccgctg gaattggcat catccagatt
    11941 gacgaaccgg ctctgcgcga aggtttaccg ctgcgtcgta gcgactggga tgcgtatctc
    12001 cagtggggcg tagaggcctt ccgtatcaac gccgccgtgg cgaaagatga cacacaaatc
    12061 cacactcaca tgtgttattg cgagttcaac gacatcatgg attcgattgc ggcactggac
    12121 gcagacgtca tcaccatcga aacctcgcgt tctgacatgg agttgctgga gtcgttcgaa
    12181 gagtttgatt atccgaatga aatcggtcca ggcgtctatg acattcactc gccaaacgta
    12241 ccgagcgtgg aatggattga agccttgctg aagaaagcgg caaaacgcat tccggcagag
    12301 cgcctgtggg ttaacccgga ctgtggcctg aaaacgcgcg gctggccaga aacccgcgcg
    12361 gcactggcga acatggtgca ggcggcgcag aatttgcgtc ggggatgatg aatattaacg
    12421 agtcactgta aattgcagta cgatgaaaga ggcaaaactg cctgatacta ggcctacata
    12481 atttatgcaa tttattgaat ttttgggttt tgttggccgg acaaggcgtt cacgccgcat
    12541 ccggcatgaa caacgcgcac tttgccaata atctgatggc ttccatcacc ggaagccatt
    12601 ttgctatctg cgcccgttca gatacagaaa taaccgaatc atttttacgc aaaatataat
    12661 cctgagcgat ttgttcagta atcaacgcta cgttatttat tctcactttt cacgaaattg
    12721 tatccctttt cagtaacgtg gtagtgaggc atagacttat gtccgctatc ctcaataata
    12781 accaaaccct cgttagctaa tttgttaaca atgttaacaa cattaccgaa tcctggaatt
    12841 tgccttatgg atagtgtgcg atcgagattc atccagttcc agtttatatc tttctcagcc
    12901 ataattttca gtacgttaat ttcagtttca gttatcatag tcaagtgcat cctggtggta
    12961 taagtcatta agttctccct taagtttata atcttctaga gtagcagtat gcgta
//
LOCUS       DAZPJR010000117        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPJR010000117 DAZPJR010000000
VERSION     DAZPJR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.118"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHVB7_003043"
     CDS             <1..191
                     /locus_tag="ACHVB7_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914964.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHVB7_003044"
     CDS             191..1393
                     /locus_tag="ACHVB7_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR1914965.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHVB7_003045"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHVB7_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR1914966.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHVB7_003046"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHVB7_003046"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1914967.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHVB7_003047"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHVB7_003047"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR1914968.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHVB7_003048"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHVB7_003048"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR1914969.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHVB7_003049"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHVB7_003049"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR1914970.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHVB7_003050"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHVB7_003050"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR1914971.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHVB7_003051"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHVB7_003051"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR1914972.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3577 g         3153 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggcgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc atcaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gattgcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctagtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgcgggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZPJR010000118        12439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPJR010000118 DAZPJR010000000
VERSION     DAZPJR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.119"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..70)
                     /locus_tag="ACHVB7_003052"
     CDS             complement(<1..70)
                     /locus_tag="ACHVB7_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914973.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLK"
     gene            689..1459
                     /locus_tag="ACHVB7_003053"
     CDS             689..1459
                     /locus_tag="ACHVB7_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR1914974.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="ACHVB7_003054"
     CDS             1450..1923
                     /locus_tag="ACHVB7_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR1914975.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="ACHVB7_003055"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="ACHVB7_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR1914976.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="ACHVB7_003056"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="ACHVB7_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR1914977.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /gene="yjjA"
                     /locus_tag="ACHVB7_003057"
     CDS             3359..3853
                     /gene="yjjA"
                     /locus_tag="ACHVB7_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR1914978.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="ACHVB7_003058"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="ACHVB7_003058"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR1914979.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="ACHVB7_003059"
     CDS             complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="ACHVB7_003059"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR1914980.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7698..8612
                     /locus_tag="ACHVB7_003060"
     CDS             7698..8612
                     /locus_tag="ACHVB7_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR1914981.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8827..10178
                     /locus_tag="ACHVB7_003061"
                     /pseudo
     CDS             8827..10178
                     /locus_tag="ACHVB7_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="ACHVB7_003062"
                     /pseudo
     CDS             complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="ACHVB7_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12001..>12439)
                     /gene="hpaR"
                     /locus_tag="ACHVB7_003063"
     CDS             complement(12001..>12439)
                     /gene="hpaR"
                     /locus_tag="ACHVB7_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR1914982.1"
                     /translation="SLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESPSMD
                     FHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRAQTQ
                     IEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         3062 a         3265 c         2994 g         3118 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     7021 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     7081 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     7141 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     7201 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     7261 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     7321 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     7381 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7441 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7501 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7561 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7621 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7681 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7741 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7801 cggaaatgta caacatcagc cgcaccacag tgcgtcatat tctcagccac ttacgcgaat
     7861 gcggcgtctt gacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7921 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7981 ttttcacgat gataaaccag cgccagttac gcccagggga aacgttttct gaactgcaac
     8041 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     8101 gttataatct tattcacagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     8161 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     8221 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     8281 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     8341 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     8401 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8461 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8521 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8581 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8641 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8701 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8761 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
     8821 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8881 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8941 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     9001 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     9061 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     9121 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     9181 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
     9241 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     9301 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     9361 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9421 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9481 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9541 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
     9601 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9661 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9721 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
     9781 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9841 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9901 gttctgctga ttggcatggc actgttctgt attcaacatc ctgctggggc ttgatccacg
     9961 tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt gccagcttca
    10021 tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc cactcattcc
    10081 gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg tacatcttcc
    10141 tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc cgataacatt
    10201 ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc gctatctgcc
    10261 acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga tgtttcacgc
    10321 tgctgttgct gaatccggaa cactgccact gcttcggtca ggcgactggc ctgctcttcc
    10381 agcgcggcgg cggcagcggc agactcttcc accagcgcgg cgttctgttg agttacccgg
    10441 tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca
    10501 gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat ctccgccatt
    10561 gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca
    10621 atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt acgcacttct
    10681 cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc aaccgccgcg
    10741 ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat aatatcggcg
    10801 attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc taccactttg
    10861 ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg gcgcgcattt
    10921 tcggcgttct gcttcaccgt tgcggtcagt tactccatgc tggctgccgt ctcttccagc
    10981 gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc gatttcgctg
    11041 gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct
    11101 ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca
    11161 atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt
    11221 ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg
    11281 atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt atcgctgacg
    11341 gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg
    11401 tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaacagt
    11461 tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct
    11521 gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca gtttttttcc
    11581 gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca
    11641 atattgttct gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc
    11701 aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt ttgtaaaaca
    11761 gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga tgtcagttgt
    11821 aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat acgttttaac
    11881 atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat
    11941 gaaaaggtgt aacccaccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag
    12001 ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa taaattcctc
    12061 taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata
    12121 ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggttagcga
    12181 gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc
    12241 catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc
    12301 gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt
    12361 caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag
    12421 caacgcaatg gttagtgag
//
LOCUS       DAZPJR010000119        12438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPJR010000119 DAZPJR010000000
VERSION     DAZPJR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.120"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            5..910
                     /gene="yhfZ"
                     /locus_tag="ACHVB7_003064"
     CDS             5..910
                     /gene="yhfZ"
                     /locus_tag="ACHVB7_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR1914983.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            927..1289
                     /locus_tag="ACHVB7_003065"
     CDS             927..1289
                     /locus_tag="ACHVB7_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR1914984.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            1373..2536
                     /locus_tag="ACHVB7_003066"
                     /pseudo
     CDS             1373..2536
                     /locus_tag="ACHVB7_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            2536..3762
                     /locus_tag="ACHVB7_003067"
                     /pseudo
     CDS             2536..3762
                     /locus_tag="ACHVB7_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            3759..4637
                     /locus_tag="ACHVB7_003068"
     CDS             3759..4637
                     /locus_tag="ACHVB7_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR1914985.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            4648..5001
                     /locus_tag="ACHVB7_003069"
     CDS             4648..5001
                     /locus_tag="ACHVB7_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR1914986.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            5013..6317
                     /locus_tag="ACHVB7_003070"
     CDS             5013..6317
                     /locus_tag="ACHVB7_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR1914987.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            6329..7414
                     /locus_tag="ACHVB7_003071"
     CDS             6329..7414
                     /locus_tag="ACHVB7_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR1914988.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(7566..8297)
                     /gene="frlR"
                     /locus_tag="ACHVB7_003072"
                     /gene_synonym="yhfR"
     CDS             complement(7566..8297)
                     /gene="frlR"
                     /locus_tag="ACHVB7_003072"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR1914989.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(8397..9182)
                     /gene="frlD"
                     /locus_tag="ACHVB7_003073"
     CDS             complement(8397..9182)
                     /gene="frlD"
                     /locus_tag="ACHVB7_003073"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR1914990.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(9179..10010)
                     /gene="frlC"
                     /locus_tag="ACHVB7_003074"
                     /pseudo
     CDS             complement(9179..10010)
                     /gene="frlC"
                     /locus_tag="ACHVB7_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(10060..11082)
                     /gene="frlB"
                     /locus_tag="ACHVB7_003075"
     CDS             complement(10060..11082)
                     /gene="frlB"
                     /locus_tag="ACHVB7_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR1914991.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTEC
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(11103..>12438)
                     /gene="frlA"
                     /locus_tag="ACHVB7_003076"
     CDS             complement(11103..>12438)
                     /gene="frlA"
                     /locus_tag="ACHVB7_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR1914992.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGELIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT         2829 a         3151 c         3536 g         2922 t
ORIGIN      
        1 tattatgcgt cgaacgttta tcaaaaaaga aggcgtcgtc atcacgacgc tggcccgtta
       61 tttgttgggt gaaaaatgcg gtaatcgact gaaaaccata gatgagctgg caactgaatg
      121 ccgttcatcg gttggcctga cgcaggccgc gttgaaaacg ttggaatcaa gcggagcgat
      181 acggattgaa cgccgtgggc gcaatggcag ttatctggtc gagatggata acaaagcatt
      241 gctgagtcat gtggatatca acaacgtggt atgtgcgatg cctttgccct atacccgctt
      301 gtacgaaggt ttagcgagcg ggctgaaagc tcagtttgat ggcattcctt tttactatgc
      361 gcatatgcgc ggcgcggata ttcgcgtgga gtgtttactt aacggcgtgt atgacatggc
      421 ggtggtttcc cgactggcgg cggaaagtta tctcacgcaa aaagggttat gcctcgcgct
      481 ggagttgggg ccgcacacct acgttggcga gcaccagttg atttgccgta aaggcgagtc
      541 cgcaaacgtg aagcgcgtgg ggctggataa ccgttcggcg gatcagaaaa tcatgaccga
      601 tgtctttttt ggcgatagtg atgtggaacg tgtcgatctc tcttatcacg agagtttaca
      661 acgcattgtt aaaggcgatg tcgatgcggt tatctggaac gtggtggcgg aaaacgaact
      721 gaccatgctg ggattagagg cgacgccgct cacagacgat ccgcgatttt tacaggccac
      781 cgaagccgtg gtcctgacgc gagtcgatga ttacccaatg caacaactgc tgcgtgccgt
      841 tgtagataaa cacgccctgc tcgcccatca acaacgggta gtgagtgggg aacaggaacc
      901 gagttattaa acgaaaggca tctgatatgg aaaccagact caacctgctt tgcgaggcag
      961 gcgttattga taaggacatc tgcaaaggca tgatgcaggt cgtcaacgta ctggaaacag
     1021 agtgccatct gccggtgcgc agtgagcaag gaacgatggc gatgacacat atggcgagtg
     1081 cactgatgcg cagtcgccgt ggtgaagaaa tagagccgct ggataacgaa ttgctggcag
     1141 agctggcgca atccagccac tggcaagccg ttgtgcaatt gcatcaggtg ttgttgaagg
     1201 aattcgcact ggaagttaac ccgtgtgaag aaggctattt gctggccaac ctttatggat
     1261 tatggatggc tgctaacgaa gaggtttgaa gctcccgaca aatccacgtc gcacaaccta
     1321 aatattcaaa aaaatgaata tttaattcaa ggaacagaaa cgaggcaaca agatgtttgt
     1381 agaagcattg aaacgccaga acccggcgct gatttccgcc gcactaagtt tgtggcagca
     1441 gggcaagatc gccccggaca gctgggtgat cgacgtggat caggtactgg aaaacggtaa
     1501 gcgactgatt gagacggcgc ggctttacgg cattgaactg tatctgatga ccaagcagtt
     1561 tggccgtaac ccatggctgg cggaaaaatt gctggcatta ggttacagcg gcattgtggc
     1621 ggtggattac aaagaggcgc gagtcatgcg ccgcgctggt ttgtcggtgg cacatcaggg
     1681 gcatctggta caaatccctt gtcatcaggt tgctgacgcc gttgaacagg gcaccgacgt
     1741 catcaccgtg tttactctcg acaaagcgcg ggaagtttct gcggcagcgg tgaaggccgg
     1801 acgtgtgcag tttgtgctgc tcaaagttta tagcgacgat gattttcttt atccggggca
     1861 ggagagcggt tttgtacagc attcgctgca cgaggtggtt gctgaaatta agaaactgcc
     1921 ggggctgcat ttagccggac ttacccattt cccttgcctg ctttgggatg aggctgccgg
     1981 aaaagttttg ccgacaccga atcttcacac gctgatacag gcacgggatc aactggcgaa
     2041 atctggtatt gcacttgagc aactgaacgc gccttcagcg accagctgca cttcgctgcc
     2101 attactggcg taatacggtg tgactcatgc cgaacccggt catgcgctga cgggcactat
     2161 tccggcaaac cagcagggcg atcagtctga acgtatcgcg atgctctggt tgagtgaaat
     2221 ctcccatcat ttccgtggcg acagctactg ctacggcggc ggttactacc gtcgtggtca
     2281 tgcgcaacat gcgttggtgt ttacgccaga aaatcaaaag attactgaaa ccaatctcaa
     2341 aactgtggat gacagcagta tcgactacac cctgtcgctg gcaggcgagt ttccggtaag
     2401 cagtgcagtg gtgctctgtt tccgcacgca gatttttgtc acccgtagcg atgtggtgct
     2461 ggtgtccggt attcaccgtg gcgaaccgaa aatcgttggt cgttatgaca gtcttggaaa
     2521 ctctctgggg gcgtaatggc gcgatttgtg gtgttagtga ttgatagctt tggcgtaggg
     2581 gcaatgaaag atgtcacgct ggtgcgtccg caagatgcgg gagcgaatac atgtggtcac
     2641 atcctgagcc agttgccgca tttgcagcta ccaacgctgg agaagctggg gctaatcaac
     2701 gcattgggtt atgcgccagg cgatatgcag ccgtcagatt ccgcaacctg gggcgtggca
     2761 gagctgcaac atgaaggtgg cgataccttt atggggcatc aggaaatttt aggcacgcgc
     2821 ccgttaccgc cgctgcggat gccttttcgc gatgtgattg accgtgttga gcaggcatta
     2881 gtatccgccg gttggcaggt ggagcgccgt ggcgatgaac tgtaatttct gtgggtcaat
     2941 caggcggttg cgattggcga taatctcgag gcggatttag gccaagtcta taacattacc
     3001 gccaatctct ctgtgatctc ttttgacgac gcaatcaaaa ttggtcgtat cgtgcgtgag
     3061 caggtacagg tcggtcgggt cattacattt ggtggcctgt taaccgacag tcaacgcatt
     3121 ctcgatgccg cagaaagcaa agaagggcgc tttattggta tcaatgcgcc gcgttctggc
     3181 gcttatgaca acggcttcca ggtcgtgcat atgggctacg gtgtcgataa aaaagtgcag
     3241 gtgccgcaaa aactgtatga ggcgggcgtg ccaaccgtgc tggtgggtaa ggtggcagat
     3301 atcgtcagca atccttatgg cgtgagctgg caaaacctgg tggatagcca gcggattatg
     3361 gatatcaccc tcgacgaatt taacacccat ccgacggcgt ttatttgcac caatatccag
     3421 gaaaccgacc tcgctggtca tgcagaagac gtcgcacgtt atgccgaacg tttgcaggtc
     3481 gttgaccgta accttgcccg gcttgttgat gcgatgcagc cagatgattg cctggtcgtg
     3541 atggcggatc acggcaacga tccgaccatt ggtcacagcc accatacccg cgaagtggtg
     3601 ccgctgctgg tttatcagca aggcctggct gctacgcagc tcggtgtgcg caccacgctt
     3661 tctgatgtgg gagctaccgt gtgtgaattt ttccgtgcgc caccgccaca aaatggtcgc
     3721 tcttttcttt cctccctccg gtttgcagga gacaccctat gagttttgat ccgacgggtt
     3781 acaccctggc ccatgagcat ctgcatattg atctctccgg ctttaaaaac aacgtggact
     3841 gccgccttga tcagtatgcg ttcatttgcc aggagatgaa cgacctgatg acccggggtg
     3901 tacgtaatgt gattgagatg accaaccgtt acatggggcg caatgcgcaa tttatgctcg
     3961 gtgtaatgcg cgaaaccggg atcaacgtgg tggcctgtac tggttattac caggatgcgt
     4021 ttttcccgga acatgtggtg acccgcagcg tgcaggaact ggcgcaggag atggtcgatg
     4081 aaattgaaca gggtatcgat ggcacggagc tgaaagccgg gatcatcgcg gagatcggct
     4141 ccagcgaagg aaagattacg ccgctggaag agaaggtatt tattgccgct gcgctggcgc
     4201 ataaccagac cggacgcccg atctccacgc atacgtcgtt cagcaccatg gggctggaac
     4261 aactggctct gttgcaagcc cacggagttg atctttcgcg cgtcaccgtt ggtcactgcg
     4321 atctgaaaga caacctcgac aacattttga agatgatcga tctcggcgcg tacgtgcagt
     4381 tcgacaccat tggtaagaac agttactacc cggacgaaaa gcgcattgcg atgctccacg
     4441 cgctacgtga ccgtgggttg ctgaaccgcg tcatgctgtc gatggatatt acgcgccgct
     4501 cccatttaaa agccaatggt ggttatggct atgactattt attgaccacc tttattccgc
     4561 aattgcgcca gtcaggattc agtcaggccg atgtggatgt gatgttacgt gaaactccct
     4621 ctcaattttt ccaataagga cagactcatg aaaaagattg gcgttgcagg cttacagcgt
     4681 gagcagatta aaaaaaccat tgaagcgacg gctactggca gttttgaagt tttcattcac
     4741 aacgacatgg aagcggcaat gaaagtgaaa tccgggcaac tggattacta catcggtgcg
     4801 tgtaataccg gtgcgggcgc ggcattgtcg attgccatcg cggtgattgg ctataacaaa
     4861 agttgcacca ttgccaaacc aggcattaaa gcgaaagacg agcatatcgc caaaatgatc
     4921 gctgaaggaa aagtggcgtt tggcctttcc gttgagcacg tcgaacacgc gattccgatg
     4981 ctgattaacc atctgaaata aaaggcacga ctatggatct gtatattcag attatcgtgg
     5041 tggcgtgcct gacgggtatg acatcgcttc tggcgcatcg ctcggcggct gtttttcatg
     5101 acggtatccg cccgatcctg ccgcaactga ttgaaggcta tatgaaccgt cgcgaggcgg
     5161 ggagtatcgc ttttggtctg agcattggtt ttgtggcctc ggtggggatc tcttttaccc
     5221 tgaaaaccgg gctgctcaac gcatggttac tctttcttcc taccgatatc ctcggcgtac
     5281 tggcgataaa cagcctgatg gcgtttggtc ttggcgctat ctggggcgtg ttgatcctta
     5341 cttgcctgtt gccagtaaac cagctgctga ccgcgctgcc ggtggatgta ttaggtagcc
     5401 tcggggaatt aagctcgccg gtggtttctg cttttgcact gttcccgctg gtggcgattt
     5461 tctaccagtt tggctggaag caaagtctgg tcgccgccgt ggttgttctg atgacccgtg
     5521 tggtagtcgt gcgctatttc ccacatctta accctgaatc catcgaaatc tttattggca
     5581 tggtgatgct gctggggatc gcgataactc acgacctgcg tcatcgtgat gaaaatgata
     5641 ttgatgccag cgggctttcg gtgtttgaag aacgcacgtc gcggattatc aaaaacttac
     5701 cctatatcgc catcgtggga gcattgattg ccgccgttgc cagcatgaag attttcgccg
     5761 gcagtgaagt gtcgatcttc actctggaga aagcgtactc cgcaggcgta acgccggaac
     5821 aatcgcaaac gctgatcaac caagcggcac tggcagaatt tatgcgcggg ctgggttttg
     5881 taccgttgat tgccaccacc gccttagcaa cgggtgtgta tgcagttgcg ggctttacct
     5941 ttgtttatgc ggtgggctat ctctcgccga atccgatagt tgcagcggta ttaggcgcag
     6001 tggttatttc ggcggaagtc ttgctgcttc gttcgatcgg caaatggctg ggacgctacc
     6061 cgtcggtgcg taatgcgtcg gataacatcc gtaacgccat gaatatgctg atggaagtgg
     6121 cgctgctggt cggttcgatt ttcgcagcaa ttaagatggc gggttatacc ggattctcta
     6181 tcgcggttgc catttacttc ctcaacgaat ccctgggccg tccggtacag aaaatggcgg
     6241 caccggtcgt ggcagtaatg atcaccggta ttctgctgaa tgttctttac tggcttggcc
     6301 tgttcgttcc ggcttaagga aggcacctat gaagacgttt cctctgcaaa gtctgacgat
     6361 tattgaggcg cagcaaaagc agtttgcgct ggtggatagc atttgtcgcc atttccccgg
     6421 cagcgagttt cttactggcg gtgatttagg cttaacgcca ggactgaatc aaccgcgcgt
     6481 tacccagcgt gtggaacagg tgctggctga tgcatttcac gcacaggctg cggcgctggt
     6541 gcagggtgcg ggtactggcg cgattcgtgc ggcgttggcg gctttgctca aaccggggca
     6601 gcgtcttctg gtacatgatg cgcctgttta cccgacgaca caggtcatta ttgagcagat
     6661 ggggctgacg cttattactg ctgatttcaa tgacctgtcg gcactgaagc aggtcgtcga
     6721 cgagcaacta ccggatgcgg cgctggtgca gcatacgcgc cagcagccgc aggacggcta
     6781 cattctggca gatgtgctgg caacattgcg ctcggctggc gttcctgcat taaccgatga
     6841 caactatgcg gtgatgaaag tggcgcgaat tggctgcgaa tgcggggcaa atgtctcgac
     6901 tttttcctgc ttcaagctgt ttgggccgga gggtgttggc gcggtggtag gcgatgccga
     6961 tgttattagt cggattcgct ccacgctcta ttccggcggc agccagattc agggcgctca
     7021 ggcgctggaa gtcttgtgtg ggctggtgtt tgcgccagtg atgcacgcgg tgcaggcggg
     7081 ggtatctgaa cggttgctgg ctttgcttaa cggtggtgcg gtgccggaag tgaaaagcgc
     7141 ggtgattgct aatgcgcagt cgaaggtgtt gattgtcgag tttcatcagc cgattgccgc
     7201 cagagtgctg gaagaggcgc aaaagcgcgg tgccttgcct tacccggtgg gtgcagagtc
     7261 gaaatatgaa atcccgccgc tcttttatcg cctttccgga acgtttcgcc aggcgaatcc
     7321 acaatcagaa cattgtgcga ttcgcattaa cccgaatcgc aacggtgaag agacggtgct
     7381 gcggattttg cgtgagagta ttgccagtat ttaatgctgg atagcaccgt caagctaaat
     7441 tccgtactga acggtcccct cgcccctttg gggagagggt tagggtgagg ggaacgtacg
     7501 ggatgtaaaa ttcaacgcgc cagcatcgcc ggatgcgatg ctggcgcatc ttatccggcc
     7561 tacggttaat gtcttttatt atcaatcgtt aaggtgatgc gattggcgcg gcagaagagt
     7621 tcggagacgt gcaccggttt gtcattctga tcaaaggcga ttttgctgat gcgaaacaac
     7681 ggttcgccca gttcgcattt cagccatttt gcctgcgggc gggtggcggc aaagatatcg
     7741 atggtctttt tgtcgctgac cactcgcgtg tcaaaacgct cctgaaataa ctgataggtg
     7801 gagcttcctt cgacgtaaat ctcatcaaag tcaggataac gcgacagcgg gatccatgaa
     7861 ctatcaataa acagcggctc tttatcgaga tacatcaccc ggcagagatg gaacacttcg
     7921 ctgtttccgg ggatgttcag cttttcgcaa aacggcgcgg cgctgacccg ttcctgttcg
     7981 atcactttct ctttcgtcgc cttgccttgt gagacgccaa aatcggtaaa accactgaca
     8041 gtaagcaggg cgttttcaac tttctggctt tgtacaaagg tgcctttgcc ctgccagcgg
     8101 atcagtacgc cgtctgccac taagtcgctg atagctttgc gaatagtaat gcggctgacg
     8161 ttatattgcg tacaaagctc gttttcggta gggatctgtt gcccggcctg gtaaaccccc
     8221 tgcgcgatat catccagcag ccgctggcgg acggtagcgt agaggagttg atgagagtag
     8281 cggtccgtag ctgacataat cgaccctgaa ataaagaatg aattacacgg attatagggt
     8341 cgttttccgg caagtaacag cacgttggcg cggagatgct cacgccaacg ttatacctac
     8401 caggcaccgt gatactgaat ggttttcgcc gcgcacgccg ttccctgcgc catcgcctgc
     8461 ggtaatgtca tccccgcaga ccagccgcaa aggaatccgg caatgaacga atctccggca
     8521 cccatggtgt cgataaccgt caccggttca ggagcctgac gccagaactg cgcgccatcc
     8581 caggcaatgc tgccgttttc acccagcgtg acaatcactg ttcctgcgcc acgggcaaca
     8641 atcgctttca tcttcagacg cagcgcttcg tcttcttgcg gtgcggaggc aaaggcaaaa
     8701 tcgagatgcg gcaccagtgt ctgccagagc gggctgtccc acttgtcgga gaagtcgaaa
     8761 gcggtaagtt tgcccgcagc gtgcagctgt gggaatgcgt cttccgcatg tccccagatt
     8821 gccgcgtgca caatgtcata ctgcgccagc caggcgtaat cctcttcact cagggcaaag
     8881 tcggccatca cgccttcggt gtagtcgcca aaaacgcgat cattgtcgtg cagttccacc
     8941 tgagtttgtg cggtaacgcc gtgtttcgta tggacatggc tgatatcgac gcccatgcgg
     9001 gcgagatcct gcttcagctt tgtgccgtag tcatcgtcac ccaccaaggt aatgcatccc
     9061 ggctgtatgc cgtagcgagt gcagtacacc gccacattga ccgcattacc gccagaaaac
     9121 gctttattca gttgcgggta gatatcgacg cagttatcgc cgattgtcgc cagggttttc
     9181 atctctcatc ctccggcagc agcgcgcgaa agcgttccag cgcctggcgg gcatagagtc
     9241 tgggctcgtt catatacatc gtcaccagct ccaccgtaca gtaaccctca tagccccgct
     9301 caataatatc gcgcatcagt tcccgcagcg gcattttgcc ttctccagga atgtaatgcg
     9361 tgtcgctggc cccgtcgctg tcgacaatat gcagatgacg ataatttatc gcccagtttg
     9421 tcgaaataac tcatcaccgg ttccgcctgg acatacggcg cgcaaatgtc gaccatgctg
     9481 aacaggcgcg gcgaaggcac cagcgccagc gcatgaagca catcattagc attacacacg
     9541 acgttcgatt catacggcgt taacggttcg aggattagat ccatgccgat gttttccgcg
     9601 tactcacaca gttcgcttag gttctctgcc agccgtcccc agataacatt aggtggcgtg
     9661 agatagcctg cgtgggccgc ggaaatcagc gtataacccg cgttcatctc ttttgccata
     9721 tccatcgcca gcttgatcat gtcgaggctt tcgcgacgca tatgttcatc gcccagcatc
     9781 atgttatacg gatagccgtt ggtttctggc gtatagccga taatcggcat ctgatacgtc
     9841 tgcgccagcg ccttgatttg tttgatgccg cccgctttta agtccggcgc gaacgcgtgc
     9901 gggcgaccgc cccaaatttc gatgccgtcg taacccagct cgcttgcatc acgaaatgcg
     9961 tgttcaatag gcagccgctg gtggccgcag gtaaacatac ctgttttcat cgggtaactc
    10021 cttgcgtaaa tccggcccgt tgccgggccg ggagagggat taatattcca ccagaccacc
    10081 gtaatagcgg cgttcatccg ggttgtgatc tttgtaaata gacaggtagt agcagagcca
    10141 ctccattggc acgaacatca ggaacggtgc cagccacggg tgcagccctt gcgaaatttc
    10201 ggcgtaatcg atgacgatca cgttgtcagt acgctgttta acaaagttaa tggcgcattc
    10261 ggtggtgtgg cgactttcat cattgccgag caggaacagg aacggaacgc ccggttcgac
    10321 aatctccagc gggccatggc ggaactctcc gctctcaatc acgcagccgt gcgtccaggt
    10381 aaattccatc agcgttacaa tgccttcttt gtaacccagc ggacgcagcg gacccgcagc
    10441 aacggtataa atcatcggcc actggctggc cagttcacca agctggcggc ctttttcttc
    10501 ccaggtgcgt accagatgac cgagcgcatt cggcaacggc ttgagatcgt ttttgatctt
    10561 gccgatttcc gcgttcggcg cgaggcgggt gatcatctcc agcaccacgc tgtagcagag
    10621 cagcaggtga atttcccaga tacagtcggc ctgataatca atgctaaatt ccgccgccga
    10681 ggtaatcggg ctgtccgcgc gtttggtgaa cgccgcagtg agtgcgccgc aggcccggcc
    10741 cagctccagc gctttgatta cctcttccgt tttaccgtag tcagaaacgc caatcaccgc
    10801 gcaacgatcg tcgaggcgat acggggtgtt atcgcagaac tcccagccgg aaatggcgta
    10861 gacctgaaga tcggaaaagc gatccgccag atgtttcgcc gtttgcgccg cgttgagtgg
    10921 cgagccgcag gcaacgaaat aaatacgatc aatgtcgcgc ttcaccatct cttccacgat
    10981 ggcgtgcacc agcggaacgt catggctgag aactttttcc acttcctgaa ccatattttc
    11041 ggtgaccaga aagtccacgg tgcttttatc aatatccaac attttatttt ctccagataa
    11101 cattacgaca aagcgttgag ctggcggcta cgcttcgccc agaaggcgta agcaggcaga
    11161 ccagtagcaa taacgatgac ggcgcagata aggccgggaa tcggtgccca gacaaaggtt
    11221 gaggcgacca gaatgaggct tgacgcaatg gcgagggtgg tcatcagccc gaaagccgga
    11281 gtacgccaca gcggtttgta atcgtcgcgt ttacgacacc agatgatgga gccgaaggtg
    11341 agggtatttt tgaaacacat caccagggtg aaataaccca gcaggctggt gagatcggaa
    11401 acgaagatga agaagatccc cagcgcccct tgcaggatga tggagacatc cggcgtgttg
    11461 tatttcggat gcacatggcc gaagcattta aagaacaggt tgtctttcgc catcgcgtat
    11521 tccagccgcg gctggtacat cacgcagctg gaaagcgaac caagaatgac gatcatcgcc
    11581 gtgatggcaa caaagatccc agcggtgctg ccgagtgcgg ggatccaggt cagggcgtcg
    11641 gaaatcggcg tttcagaatt ggcgagtttg tcgaagggca tcaggccgga aatcaccagc
    11701 gccagcaggg tgtagagcac cagaaccagc agacaggaac caatcagcgc tcgtggcatg
    11761 gtttttccgg ggtttttaat ttcgccggtc atataacaga tagaggccat gccggtatac
    11821 gaccaactgg tggcagagat ccccgccagc agcgccataa agctgcccgt tgcgccaatc
    11881 gcagtggtgg taggggcggc aaaattctcc gctttaaacc agaagatccc aaggccaatg
    11941 acgatagtga acgggataat tttggcgatg gtaattagcg tctgaaacgc tgcgccgcct
    12001 tcaacggagc gcaggtgtag caacataaag gcgataatta atccggcggc gataaattta
    12061 ccgagcaacg gatcgatagg cgttaaaaag ccaagattgc tgacaatcgc cagcgccata
    12121 atcgacaatg acggcgcatc gttggcccag aagctggccc agccggagag gaaagccagc
    12181 ggtcggcttc cggcattttt cagataaaca taatctgcgc cattttccgg ataagcggtg
    12241 gatagttccg catagacgca catttgcggg atcacaatta actcgccaat gacaaacgcg
    12301 agcaccgtaa gccacggcgt gcccgctgct tttgccactt cacccacaga tacaaaaata
    12361 ccggagccga cggttgtccc gacggcgatt gcaagaacgg cccaaaatcc gagcttgcgt
    12421 tggagttcct ggcttccc
//
LOCUS       DAZPJR010000120        12383 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPJR010000120 DAZPJR010000000
VERSION     DAZPJR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12383
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.121"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..347)
                     /locus_tag="ACHVB7_003077"
     CDS             complement(<1..347)
                     /locus_tag="ACHVB7_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1914993.1"
                     /translation="MGREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGS
                     EFRIRDINENFVG"
     gene            complement(624..887)
                     /gene="cedA"
                     /locus_tag="ACHVB7_003078"
     CDS             complement(624..887)
                     /gene="cedA"
                     /locus_tag="ACHVB7_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR1914994.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            1070..3331
                     /gene="katE"
                     /locus_tag="ACHVB7_003079"
     CDS             1070..3331
                     /gene="katE"
                     /locus_tag="ACHVB7_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR1914995.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3589..4347)
                     /gene="chbG"
                     /locus_tag="ACHVB7_003080"
     CDS             complement(3589..4347)
                     /gene="chbG"
                     /locus_tag="ACHVB7_003080"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR1914996.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(4360..5712)
                     /gene="celF"
                     /locus_tag="ACHVB7_003081"
                     /gene_synonym="chbF"
     CDS             complement(4360..5712)
                     /gene="celF"
                     /locus_tag="ACHVB7_003081"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1914997.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5817..6659)
                     /gene="chbR"
                     /locus_tag="ACHVB7_003082"
     CDS             complement(5817..6659)
                     /gene="chbR"
                     /locus_tag="ACHVB7_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR1914998.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6667..7017)
                     /gene="chbA"
                     /locus_tag="ACHVB7_003083"
     CDS             complement(6667..7017)
                     /gene="chbA"
                     /locus_tag="ACHVB7_003083"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR1914999.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(7068..8426)
                     /gene="chbC"
                     /locus_tag="ACHVB7_003084"
     CDS             complement(7068..8426)
                     /gene="chbC"
                     /locus_tag="ACHVB7_003084"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR1915000.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(8511..8831)
                     /gene="chbB"
                     /locus_tag="ACHVB7_003085"
     CDS             complement(8511..8831)
                     /gene="chbB"
                     /locus_tag="ACHVB7_003085"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR1915001.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(9130..9468)
                     /gene="osmE"
                     /locus_tag="ACHVB7_003086"
     CDS             complement(9130..9468)
                     /gene="osmE"
                     /locus_tag="ACHVB7_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR1915002.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9670..10497
                     /gene="nadE"
                     /locus_tag="ACHVB7_003087"
     CDS             9670..10497
                     /gene="nadE"
                     /locus_tag="ACHVB7_003087"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR1915003.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10727..11614
                     /gene="cho"
                     /locus_tag="ACHVB7_003088"
     CDS             10727..11614
                     /gene="cho"
                     /locus_tag="ACHVB7_003088"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR1915004.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11574..12149)
                     /gene="ves"
                     /locus_tag="ACHVB7_003089"
     CDS             complement(11574..12149)
                     /gene="ves"
                     /locus_tag="ACHVB7_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR1915005.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         3326 a         3145 c         2781 g         3131 t
ORIGIN      
        1 atcccaacaa aattctcatt aatatctctt atccgaaact cactgccata aagattatta
       61 attatcactc tatatttaac attgtcctgc agatacgtat caaaactaag tgctggagct
      121 cctggctttg aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc
      181 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
      241 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
      301 ccataattac tctgtattaa gtttgtagaa aacatctccc gccccatatt gttaacaaaa
      361 ttattatctc atttaaatct aagtcactta caatataagt ttaagagcga cgccacagga
      421 tgaactatca aaaatagctc atcatgatta gcaaaactta accattttaa aataaataaa
      481 caattaaaga aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
      541 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcatg taactcccgg
      601 caaaaccggg aagtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
      661 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
      721 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
      781 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
      841 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
      901 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
      961 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
     1021 ttgaccaaaa cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca
     1081 taacgaaaag aacctacatc agcaccagtc accactacac gattccagcg aagcgaaacc
     1141 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     1201 gccaggtgca caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa
     1261 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca
     1321 gggcgtgcgc atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct
     1381 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
     1441 acgtattgtt catgcacgcg gatcagcagc tcacggttat ttccagccat ataaaagctt
     1501 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
     1561 taggttctct accgttcagg gtggcgctgg ctctgcagat accgtgcgtg atatccgtgg
     1621 ttttgccacc aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc
     1681 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
     1741 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
     1801 tctgcaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
     1861 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
     1921 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
     1981 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
     2041 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
     2101 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
     2161 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
     2221 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
     2281 cgatccgctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
     2341 tgggccgaat ttccatgaga ttccgattaa ccgtccgacc tgcccttacc acaacttcca
     2401 gcgtgatggc atgcatcgta tggggatcga cactaacccg gcgaattacg aaccgaactc
     2461 gatcaacgat aactggccgc gcgaaacacc gccgggaccg aaacgcggcg gttttgaatc
     2521 atatcaggaa cgcgtggaag gaaataaagt tcgtgagcgc agtccatcgt ttggcgaata
     2581 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
     2641 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
     2701 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
     2761 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
     2821 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc
     2881 gattttactt aatgatgaag tgagatcggc agaccttctg gccattctca aggccctgaa
     2941 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
     3001 cggtacggtg ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc
     3061 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
     3121 cctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
     3181 taaagcaaca atcaaggtcg ctgaccaggg tgaagaaggg attgtggaag ctgacagcgc
     3241 tgacggtagt tttatggatg aactgctaac gctgatggca gcacaccgcg tgtggtcacg
     3301 cattcctaag attgacaaaa ttcctgcctg atgggaggcg gcgcaattgc gccgcctcaa
     3361 atgatttaca tagtgcgctt tgtttatgcc ggatgcggcg tgaacgcctt atccggccta
     3421 caaaactgtg caaattcaat atattgcagg aaacacgtag gcctgataag cgaagcgcat
     3481 caggcagttt tgcgtttgtc agcagtctca agcggcgcag ttacgccgcc tttgtaggaa
     3541 ttaatcgccg gatgcaaggt tcacgccgca tctggcaaac atcctcactt acacatcccg
     3601 ataactcccc aaccgataac cacgctgagc gatagcacct ttcaacgacg ctgatgtcaa
     3661 cacatccagc tccgttaagc gtgggaaaca gtaagcactc tgacggatag tattatcgat
     3721 aaacgccgga tgacacatca cctccagcga acgatcaccc cgatggcttg cgtcatccag
     3781 cacttgcagg aacagtgcct cgctaatctc ttcaccataa aacgcgctac tgaatccctg
     3841 agaactgcgc agattagccg gtaaatcacc agagttagat agcggctgac ggtcgatgcg
     3901 caacgcaatc ccctcttcag ccgcaaacct ggcaacaatc gggaaaatct gcgggaacat
     3961 atgcacatga tgatggctat caagatgcgt aggtttgcgt ccaaatagct caatgaaacg
     4021 caaatactga ctggcaagct cctgagtaat ttcttccagc ggtaaagcat cttcttctgc
     4081 caactgccag atccattttc ccagcacacc atcgcgggtt aaccccggca tagctgtcag
     4141 tggcttgccc atagtaagga caaagtacat ccctatggcc agactcggtt catcacgact
     4201 caactgcacc gcatggtcaa tagcctgccc attcaccagc gccgtcgtcg atgtgacaat
     4261 cccattgcga caggcctcga taatgccgta gttctgacct ttgcttaagc caaaatcatc
     4321 ggcattaaca atcagtaagc gttccataat cagcctcggt taatgtgctt ttttaagctc
     4381 tgcgatgcag tcggcaaaat ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc
     4441 cagcagctca gcatcgcgat cggaatgcac caacggacta aggtttagcg ccagtaacac
     4501 atcgttaaat tctccgctaa gtgcggcgtt gctggcagca atctcgaagc ctttaatggt
     4561 gtgaatcagc cccatcactt tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc
     4621 atcgcgcccc agcttacagg tcatttctac cgcccagtct gccggaatat tatcaatatg
     4681 cccatgatgc gggatattaa cgtaatgttc agcttgcttg tcgttgtaga tagcgttgat
     4741 cacttcgcac gctgcatcag agtaataagc cccaccgcgc tgttccagtt ctttcggctt
     4801 aactttcaac tccgggtttt tatacagctc aaaaagttgt ttctcgactt tctgtactac
     4861 ctgcgctcgt gcgccgcctt tgtagtattc gcccatttca atagccagca tctctttttg
     4921 cttgaagtaa taaagcaaat aagaacacgg caacagattc agagaacgaa ttaagccttc
     4981 gctaaatggc agatcgaaaa tatttttaac gccagatgct tttaactgcc cggatgccac
     5041 accatcaagc aattcggcaa agcgcgactt gccatttacc agcacatcct taatgaacac
     5101 catatggttg aggccgaaca aatcgataga taaatcatcg ctgtctttca gcatcagaac
     5161 atcgtgaata aacatcttca tgccgatcgg aatattacac acgccgataa agcgtttaaa
     5221 tccggtatga cgataaacgg cttcagtaac cattcccgcc gggttagtga agttaatcac
     5281 ccatgcattc ggacaaagtt cttcgacatc ttttacgatg tcaaaaatca ccggaatggt
     5341 acgcagacct ttaaacagac cgcccgcgcc gttggtttcc tggccaagat aaccatgact
     5401 taatggaata cgttcatcca gttcgcgcgc cggtaattgg cctacgcgca gttgggtagt
     5461 aacgaaatca gcatctttca atgcttcgcg gcgatccagc gttttataaa gcttcatcgg
     5521 gacgccagcg ttatcaatca tccgttggca gagttcaaaa atgatatcca gtttctcttt
     5581 accatcttcg acatccacca gccataattc gctgaccggc aattcgtgat aacgcttaat
     5641 aaatccttcc agtaactccg gggtatagct gctcccgcca ccaatagtga cgacttttaa
     5701 tttctggctc ataatttctc ccttcagtac agaatactga tatctggcat atctgccccc
     5761 ccggacataa ataatccagc aacaggacag atatgtgaat tgtcaggtat aacgacttac
     5821 tgattaaatt cagtcaattt cttacgatag ctcttaggcg taaaggacgt taatttctta
     5881 aacgttttaa taaacaaact cgggctacta taaccggcct caaacgcaat atccgtcacg
     5941 gaatagttgg tcatttccag ttgttttttg gcaaaattaa tacggatctc attaataatc
     6001 tgcattggtg ttttgccata atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg
     6061 gctgacaacg tcaccatatt ctccagcgcc gattcactaa actgctcttt atcatgcatc
     6121 ttttctaccg tacttttcag ccactgcggt acatcatcaa tcacctgttc ttcgcggtaa
     6181 tgacgtaaac ggttaatgac ataaaaagta accatctcaa caaactcttc cagccctgtt
     6241 tcgcggaaat tcaatgaaga aatcactgtt tccacatagg tgagaaaagc gttattggtc
     6301 cggtatacct gcgaagcgac aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa
     6361 aagcgtttac tgatcccaac gttcaatata cgcgtggcac caaactcata aaaactttga
     6421 tggtgcgaac ctaacggaat aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg
     6481 ttaatctcct ggaaataacg cccggttaat accagagtaa attcataata gtcgtgctga
     6541 tgcagcccgc tgatactctc agttttgtta tagataaaca catggaagtt tttgccatta
     6601 aacaactgct gttctcgggc agtggcaatt tccggcgcgt taatcactgg ctgcatcatc
     6661 gactccttat gccttcagtt tttcatgaag ctcaattaat tcagtaatca gttcacgcgc
     6721 aagcatggac gtcattaaat gatcctgagc gtggaccagc accagactca ctttcatctt
     6781 accttcgccc gcatcgcctt caatcagttt cgtctgtacc agatgcgctt cattcaatgc
     6841 catccgtgac tgatccatca tggcttttgc tgcagcaaaa tcgccctgct tcgcctgttt
     6901 cagtgccgca tacgccaggc tgcgcgcttg tccggagttg atgataagcc ccatcaccac
     6961 ttcttccagc tcttcagctt ccgtttgcgt atcgggaatg ttatcgagat ccatcataca
     7021 tcgttcctct ctattcttac cggcacgatt acccgtaccg gcatcgatta aaatttcagg
     7081 gcgttagcga tatcttcttc gcgctcttct ttatcaatcg cgttttgtgc tttgttagcc
     7141 accacaacaa agggcagata aattaacgtt gcgataccaa ggttgaagag tgcaaccagc
     7201 aatgcggcga cgctaccgtt ggtgttaaag aaggctccca gaccggttgg catggtccac
     7261 ggtgcaatat tggtcaccgg aggaatgatg cccatgtagt acgctgcgag ggtgattgcc
     7321 gccagaatcg gttgtaccag tacaaacggg ataaacatca ccgggttcat gataattggc
     7381 agaccaaaca gaatcggttc gttaatctgg aagatgccgg acggcagcgc cagttttgcc
     7441 acctgacgat aatcagcacg gcgagaggcg ataaagatag ccaggatcag gcctaaagtc
     7501 gcaccactgc ccccaaggaa aataaaggag tccagcatcg gcttggccca gatatggaag
     7561 gtcttaccgg ctgccagcgc cgcttcgacg gaaccatatt gctgataggt cgcgatattt
     7621 tccagcgccc acggcgtcat aatgccgttg tccagtgcgg tcagcgccag cgcgccatga
     7681 ataccgaaga accagagcag tggaacaaag atcacatagg cccagcccac cacgctaccc
     7741 aacgatgcca gtggggttga gatggtatcc ataatgatct gatggaagtt ggtgccccag
     7801 gtattcaacg cccaggcaat aatccccatc acggaaagaa taataaagcc gggaattaat
     7861 gccgagaagg aacgcgatac tgaagcaggt acgctatcgg gcaatttaat gacccaattg
     7921 cggcggacaa taaaggtaaa catttctgcc accaccaggc caataataat cccggagatg
     7981 atattcgctc cacctaacca gtttgcgcca accacatagg cctcaccgac actatatggg
     8041 gtgacggtca taaatgctgc aacggataac aacccagccg ccagcgcatc gactttacgc
     8101 tcttctgcca gcgccatgcc aataaagaac ggtgccatta aagacattat ccctaatgtt
     8161 ccgttatata cgttgccgcc aatacctttc aggccattaa gtgtttcaat ggttgaggcg
     8221 tcgaggcgaa tacctaagga ataaaaaaac gccccctccc caaagcttag aaaaacgttg
     8281 ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat aaagccattt
     8341 ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag gagtaccttt
     8401 tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca atttaaatat
     8461 tgcggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa ttaatttgct
     8521 gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca
     8581 taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc
     8641 aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca
     8701 gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg
     8761 cgcatttttg ataccagtaa agaggtagac atgcccgcag aacaaaacag ataaatgtgt
     8821 ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc
     8881 ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga
     8941 aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa attgtttctc
     9001 cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa
     9061 gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc aagctttcag
     9121 acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg tctgataacc
     9181 ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc
     9241 acgttgaccc aggatgtagg tctggcaagt accacgagca tggatcatgc tcacttcaga
     9301 cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt
     9361 caccacaggc tgtacaaact ggtctttagt acgatcataa gccgtacaac ccgccagcat
     9421 ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct
     9481 tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgcgaac
     9541 gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt cagcccgctg
     9601 tcgcttgtcg tttcagtggc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg
     9661 aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt
     9721 aatgctgaag aggaaattcg tcgtagtatc gattttctga aaagctacct gcaaacttat
     9781 ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac gcttgccgga
     9841 aagctgtgcc agatggcgat taatgagctg cgcctggaaa ccggcaacga atcactgcaa
     9901 tttattgccg tacgcctgcc ctatggtgtt caggccgacg aacaagattg ccaggatgcc
     9961 attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtattggcc
    10021 agcgaacagg cattgcggga agcaggcatt gaactgagcg attttgtccg tggcaatgaa
    10081 aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag cggtgtcgtg
    10141 gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata tggtgacggc
    10201 ggtacagaca ttaatccgct gtatcgtctc aacaaacgtc agggtaaaca gttactggcg
    10261 gcattaggtt gcccggaaca cctttataag aaagcgccaa cggccgatct ggaagatgat
    10321 cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat cgacgactat
    10381 ctggaaggga aaaacgtacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa
    10441 accgaacata aacgccgtcc gccaattacc gttttcgatg atttctggaa aaagtaataa
    10501 tttgcacata atggtttgtg cgaaaaagag taatttgttc acgccggatg cggcgtgaac
    10561 gccttattcg acctacaaaa ctatgcaaat tcaatatatt gcaggagtcg aataggcctg
    10621 acaggcgtag cacgtcagac ggtgtaacct ttgtcatcga cccgcctctt ttttaatcgc
    10681 tccccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt
    10741 aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttacg
    10801 ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga
    10861 caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc tttctcattt
    10921 acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct ggatctgtac
    10981 cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac aacagccgct
    11041 gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg
    11101 cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc tgtttggcct
    11161 gtttgccaat aggcgcgcag ctttgcaagc attgcagacc atcgctgatg aacaaaaact
    11221 ttgttatggc ctgctgggac tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc
    11281 gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac atgcgctacg
    11341 cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag gtgcggtggc
    11401 actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct
    11461 gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg ccgggtttga
    11521 tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg aaattactga
    11581 acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag tttaccttgc
    11641 ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg
    11701 agcaatttat cgcccagttg ccacgccccg ttaatcacaa acaccacccc gccgcgtgag
    11761 ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgt
    11821 gtcatgatgt tgaaatccat cgacatctgc ccttccgtca gtttcgcttt taccacctgg
    11881 tccgctgcaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg gtctgcgctt
    11941 tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc gggaaacaaa
    12001 gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa atcacgtttt
    12061 gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag catttcgcca cagattcacc
    12121 gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt ttcattgtta
    12181 tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta gtggtgatca
    12241 ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt
    12301 actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag attattcagc
    12361 agttgcaggc attttacctt ttg
//
LOCUS       DAZPJR010000121        12359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPJR010000121 DAZPJR010000000
VERSION     DAZPJR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.122"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHVB7_003090"
     CDS             complement(<1..330)
                     /locus_tag="ACHVB7_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR1915006.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHVB7_003091"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHVB7_003091"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR1915007.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHVB7_003092"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHVB7_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR1915008.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHVB7_003093"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHVB7_003093"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR1915009.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHVB7_003094"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHVB7_003094"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR1915010.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLMDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHVB7_003095"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHVB7_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR1915011.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHVB7_003096"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHVB7_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR1915012.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHVB7_003097"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHVB7_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR1915013.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHVB7_003098"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHVB7_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR1915014.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHVB7_003099"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHVB7_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR1915015.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHVB7_003100"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHVB7_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR1915016.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHVB7_003101"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHVB7_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR1915017.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHVB7_003102"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHVB7_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR1915018.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2831 a         3451 c         3021 g         3056 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccatcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaact
//
LOCUS       DAZPJR010000122        12231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPJR010000122 DAZPJR010000000
VERSION     DAZPJR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.123"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="ACHVB7_003103"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="ACHVB7_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR1915019.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="ACHVB7_003104"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="ACHVB7_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR1915020.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="ACHVB7_003105"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="ACHVB7_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR1915021.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="ACHVB7_003106"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="ACHVB7_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR1915022.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="ACHVB7_003107"
     CDS             complement(2270..2761)
                     /locus_tag="ACHVB7_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1915023.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHVB7_003108"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHVB7_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR1915024.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHVB7_003109"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHVB7_003109"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR1915025.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHVB7_003110"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHVB7_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR1915026.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="ACHVB7_003111"
     tRNA            5405..5492
                     /locus_tag="ACHVB7_003111"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="ACHVB7_003112"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="ACHVB7_003112"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="ACHVB7_003113"
     CDS             complement(5764..6450)
                     /locus_tag="ACHVB7_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1915027.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHEWADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="ACHVB7_003114"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="ACHVB7_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR1915028.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="ACHVB7_003115"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="ACHVB7_003115"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR1915029.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="ACHVB7_003116"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="ACHVB7_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR1915030.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10902..11732)
                     /gene="efeU"
                     /locus_tag="ACHVB7_003117"
     CDS             complement(10902..11732)
                     /gene="efeU"
                     /locus_tag="ACHVB7_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR1915031.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3231 a         3168 c         2830 g         3002 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg cccattcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgtaact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaagttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
    10921 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
    10981 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
    11041 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
    11101 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
    11161 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
    11221 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
    11281 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
    11341 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
    11401 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
    11461 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
    11521 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
    11581 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
    11641 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
    11701 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
    11761 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
    11821 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
    11881 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
    11941 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
    12001 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
    12061 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
    12121 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
    12181 caggacgtta cctcgcgccc gacccggtag ccgaagggga taagctgaag g
//
LOCUS       DAZPJR010000123        12037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPJR010000123 DAZPJR010000000
VERSION     DAZPJR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.124"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..323
                     /locus_tag="ACHVB7_003118"
     CDS             <1..323
                     /locus_tag="ACHVB7_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915032.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHVB7_003119"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHVB7_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHVB7_003120"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHVB7_003120"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR1915033.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="ACHVB7_003121"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="ACHVB7_003121"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR1915034.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHVB7_003122"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHVB7_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR1915035.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHVB7_003123"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHVB7_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR1915036.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHVB7_003124"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHVB7_003124"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR1915037.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="ACHVB7_003125"
     CDS             8424..8927
                     /locus_tag="ACHVB7_003125"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR1915038.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="ACHVB7_003126"
     CDS             9004..9687
                     /locus_tag="ACHVB7_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR1915039.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="ACHVB7_003127"
     CDS             9766..10752
                     /locus_tag="ACHVB7_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1915040.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="ACHVB7_003128"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="ACHVB7_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR1915041.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2862 a         3094 c         3225 g         2856 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcat
//
LOCUS       DAZPJR010000124        11959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPJR010000124 DAZPJR010000000
VERSION     DAZPJR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.125"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1759
                     /locus_tag="ACHVB7_003129"
     CDS             <1..1759
                     /locus_tag="ACHVB7_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1915042.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2095..3072
                     /gene="glaH"
                     /locus_tag="ACHVB7_003130"
     CDS             2095..3072
                     /gene="glaH"
                     /locus_tag="ACHVB7_003130"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR1915043.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3092..4360
                     /gene="lhgO"
                     /locus_tag="ACHVB7_003131"
     CDS             3092..4360
                     /gene="lhgO"
                     /locus_tag="ACHVB7_003131"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR1915044.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4383..5831
                     /gene="gabD"
                     /locus_tag="ACHVB7_003132"
     CDS             4383..5831
                     /gene="gabD"
                     /locus_tag="ACHVB7_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1915045.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5845..7125
                     /gene="gabT"
                     /locus_tag="ACHVB7_003133"
     CDS             5845..7125
                     /gene="gabT"
                     /locus_tag="ACHVB7_003133"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR1915046.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7436..8836
                     /gene="gabP"
                     /locus_tag="ACHVB7_003134"
     CDS             7436..8836
                     /gene="gabP"
                     /locus_tag="ACHVB7_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR1915047.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8857..9519
                     /gene="csiR"
                     /locus_tag="ACHVB7_003135"
     CDS             8857..9519
                     /gene="csiR"
                     /locus_tag="ACHVB7_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR1915048.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9520..9969)
                     /gene="kbp"
                     /locus_tag="ACHVB7_003136"
     CDS             complement(9520..9969)
                     /gene="kbp"
                     /locus_tag="ACHVB7_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR1915049.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10053..10211)
                     /locus_tag="ACHVB7_003137"
     CDS             complement(10053..10211)
                     /locus_tag="ACHVB7_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR1915050.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10394..10693
                     /locus_tag="ACHVB7_003138"
     CDS             10394..10693
                     /locus_tag="ACHVB7_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1915051.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10703..11227
                     /gene="ygaP"
                     /locus_tag="ACHVB7_003139"
     CDS             10703..11227
                     /gene="ygaP"
                     /locus_tag="ACHVB7_003139"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR1915052.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11274..11678)
                     /gene="stpA"
                     /locus_tag="ACHVB7_003140"
     CDS             complement(11274..11678)
                     /gene="stpA"
                     /locus_tag="ACHVB7_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR1915053.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2984 a         2947 c         3078 g         2950 t
ORIGIN      
        1 tgtatataat attaacgtta cgtcaccttc cggacagcaa gaaacattta aaatactaat
       61 caatccagaa catctacggc agacacttga gcgtaaatct cttactgctg ttcagaaatc
      121 acaatgtgaa atcatcaccc ctaaaaaacc cggcgaagcg attcttcatg cttttaatgc
      181 cacctaccag caaatcaggg aaaatatgtc tgagtttgca cgttgccatt atgggtatat
      241 acaaatccct ccagtgacaa ctttccgcgc cgacggacca gaaactcccg aagaagaaaa
      301 gggttactgg tttcatgctt atcaacccga agatctttgt accatccata atccaatggg
      361 agatttgcag gattttattg cattggttaa agatgctaaa aaatttggta tcgatatcat
      421 tcctgattat acctttaact ttatgggaat tgggggtagt ggtaaaaatg acctggatta
      481 tccctctgct gatatacgag cgaagatcag taaagatata gaaggtggta tccctggcta
      541 ttggcaaggt caggttttga ttccattcat taaagatcca gtaacaaaag aacgtaaaca
      601 aatccatcca gaagatatac atctcactgc aaaagacttc gaagcaagta aagataacat
      661 ctctaaggat gaatgggaaa acctccatgc attaaaagaa aagcgtttaa atggaattcc
      721 taaaacaaca cccaaaagtg accaggttat tatgttgcaa aatcaatacg ttcgtgaaat
      781 gcgaaaatat ggcgtacgag gtttacgtta tgatgcggca aaacactcaa aacatgaaca
      841 aatagaaaga tcaataaccc caccgcttaa aaattataat gagcggttac acaatactaa
      901 cttatttaac ccaaaatatc ataaaaaagc cgttatgaat tacatggaat atctggtaac
      961 ttgtcagttg gatgaacaac aaatgtcatc actgctttat gaaagagatg atttaagcgc
     1021 cattgatttt tcattgctca tgaagacgat aaaagccttt tcattcgggg gagatctcct
     1081 aacccttgca tcaaaaccgg gttccacaat ctcaagcatc ccatcagaaa gacggatatt
     1141 gattaacatt aaccacgatt ttcctaacaa tggcaatctt ttcaatgact ttctatttaa
     1201 ccatcaacaa gatgaacaat tagcaatggc atatatggcc gctctcccgt tcagcagacc
     1261 tttagtttac tgggatggcc aggtattaaa atcaacgact gaaattaaaa attatgatgg
     1321 gtccacgcgt gtcggtggtg aggcgtggct taataaaggt tgctctacct atcagcagct
     1381 ctacaatgaa ttccacgcat tatatataga taaagcagga atatggagcg catttgaggg
     1441 tgtatttgca actaaaaacg ttctagcctt tagtcgtggg gattctgtga acattaatca
     1501 ctctcctcat gatggactag ttataataaa taaaggaaac gaagaagttg aaggtacctg
     1561 gcctaacaaa ttgcaacctg gaatatataa aaacatgggg agtaatagcg ttaacattat
     1621 tattaataat acccgaaaaa ttatcccccc tggtaaagta tttacgctta gaggcggaac
     1681 tctaaatatc aatattcctg ggcgtagcgc tcttctttta gggaaaacag gagaaccgcc
     1741 gaactatctc tatttataat ttaatgttat atctgccccg ataaaacggg gcagataata
     1801 tgtttagttt actaacggtc attttgcagt gaagccattt actgtttttt atcgaccaga
     1861 taatctgttc tctaatgtta actcccccta acctgttgct ttagttattc atttcctgtc
     1921 tcactttgcc ttaatactct acgttaaatg ttactaattt gttgcttttg atcacaataa
     1981 gaaaacaata tgtcgctttt gtgcgcattt ttcagaaatg tagatatttt tagattatgg
     2041 ctacgaaatg agcatcgcca tgtcacccta catctcataa gaggatcgct tctgatgaat
     2101 gcactgaccg ccgtacaaaa taacgctgtc gattcaggcc aggactatag cggattcact
     2161 ctcatcccgt cggcgcaatc cccgcgtctg ctggaactca ccttcaccga acagacgacc
     2221 aaacagtttc tcgagcaggt tgccgaatgg cccgtacagg cgctggagta caaatcgttt
     2281 ctgcgttttc gggtaggcaa aattcttgac gatctgtgtg cgaatcagct gcaaccattg
     2341 ctgttgaaga ccctgctaaa ccgtgctgaa ggtgcgctgt tgatcaatgc ggtgggtatc
     2401 gatgatgtgg cgcaggcaga tgagatggtg aagctggcaa cggcggtagc gcatctgatt
     2461 ggtcgctcaa atttcgatgc gatgagcggt cagtattacg cgcgtttcgt ggtgaaaaat
     2521 gtcgataact cagacagcta tctgcgtcag ccgcaccgcg tgatggaact gcacaacgac
     2581 ggcacgtatg tcgaagagat caccgattac gtgctgatga tgaaaatcga tgagcaaaac
     2641 atgcagggtg gaaattcgtt gctgctgcat ctcgatgact gggaacatct ggaccactat
     2701 ttccgccatc cactggcgcg tcgcccgatg cgctttgccg cgccgccgag caaaaacgtc
     2761 agcaaagatg ttttccatcc agtgttcgac gttgatcaac agggtcgccc ggtgatgcgc
     2821 tatatcgacc agttcgtcca gccaaaagat tttgaagaag gcgtgtggtt gagcgagctt
     2881 tccgatgcca ttgaaaccag caaaggcatt ctttctgtac ccgttcccgt tggcaaattc
     2941 ctgttgataa acaacctgtt ctggctgcac ggtcgcgacc gctttactcc gcacccggat
     3001 ctgcgccgtg aactgatgcg tcagcgtggc tatttcgctt acgccactca ccactaccag
     3061 acgcatcagt aagcgcaaag gaattgagcg gatgtatgat tttgtgatta ttggcggcgg
     3121 catcatcggc atgtcgaccg ccatgcaact gattgatgtt tatccggatg cccgcattgc
     3181 gttgctggaa aaagagtccg gcccggcctg tcaccagacg ggccacaaca gcggcgtgat
     3241 ccatgccggg gtctattaca cgcccggcag cctgaaggcg cagttttgcc tggcggggaa
     3301 ccgcgccacc aaagcctttt gcgatcaaaa cggcattcgc tatgacaact gcggcaagat
     3361 gctggtcgcc acgtctcagc tcgaaatgga acggatgcgc gccttatggg aacgcacagc
     3421 ggcgaacggt atcgagcgcg agtggataaa cgccgtggaa ctgcgcgagc gcgaaccgaa
     3481 tatcaccggg cttggtggca tttttgtgcc gtccagcggc attgtcagct accgcgaagt
     3541 cacagcggca atggcaaaaa tcttccaggc cagaggcggc gagattattt ataacgccga
     3601 agtcagcgcc ctcagtaagc ataaaaacgg cgtggtgata cgcacccgtc agggcggtga
     3661 atatgaagca tcgacgctga ttagctgttc cgggctgatg gctgaccggc tggtgaaaat
     3721 gctcggcctc gaaccgggct ttatcatctg cccgttccgt ggtgagtatt tccgccttgc
     3781 gccggagcat aaccagattg ttaaccacct gatttacccc attcccgacc ccgcgatgcc
     3841 atttttgggc gtgcatctca cccgcatgat cgacggcagc gtgaccgtcg ggccaaacgc
     3901 ggtgctggct ttcaaacgcg aaggctatcg caagcgcgac ttctcattta gtgacacgct
     3961 ggagattttg agctcgtcgg ggattcgccg ggtgctgcaa aaccatctac gctcaggact
     4021 gggcgagatg aaaaactcgc tgtgcaaaag cggctacctg cggctggtgc aaaagtattg
     4081 tccccggctt tcgttaagcg atctccagcc ctggcccgcc ggtgtgcggg cgcaggcagt
     4141 atcgccggac ggcaagctga ttgacgattt tctgtttgtc accaccccac gcacgatcca
     4201 cacctgcaat gcgccctccc cggcagcgac atcagcaatt cctattggtg cgcatattgt
     4261 cagcaaggta caaacgctgt tggcaagcca gagtaacccc ggacgcacgc tgcgagcggc
     4321 acgtagtgtg gatgccttac acgccgcatt taatcaataa cctttgaaaa caggacgtaa
     4381 caatgaaact taacgacagt aacttattcc gccagcaggc gttgattaac ggggaatggc
     4441 tggacgccaa caatggcggg atcatcgacg tcaccaatcc ggcgaacggc gacaagctgg
     4501 gtagcgtgcc gaaaatgggc gctgatgaaa cccgcgccgc tatcgacgcc gccaaccgcg
     4561 ccctgcccgc ctggcgtgcg ctcaccgcca aagaacgcgc caacattctg cgcaactggt
     4621 tcaatttgat gatggagcat caggacgatt tagcgcgcct gatgaccctc gaacagggca
     4681 aaccactggc cgaagcgaaa ggcgaaatca gctacgctgc ctcttttatt gagtggtttg
     4741 ccgaagaagg caaacgtatt tatggcgata ccattcccgg tcatcaggcc gataagcgcc
     4801 tgattgttat caagcagccg attggcgtta ccgcagctat cacgccgtgg aacttcccgg
     4861 cggcgatgat tacccgtaaa gccggtccgg cgctggcggc aggctgcacc atggtgctga
     4921 agcccgccag tcagacaccg ttctctgcgc tggcgctggc ggagctggcg attcgcgcgg
     4981 gcattccggc tggcgtgttt aacgtagtca ccggttcggc aggcgcggtc ggtaatgaac
     5041 tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg ttcgaccgaa attggccgcc
     5101 agttaatgga acagtgcgcg aaagacatca aaaaagtgtc gctggagctg ggcggcaacg
     5161 cgccgtttat cgtctttgac gatgccgatc tcgataaagc cgtggaaggc gcgctggcct
     5221 cgaaattccg caacgccggg caaacctgcg tctgcgccaa ccgtttatac gtgcaggacg
     5281 gcgtgtatga ccgctttgcc gaaaaattgc agcaggcggt gagcaaactg cacatcggcg
     5341 acgggctgga taaaggcgtc accatcgggc cgctgatcga tgaaaaagcg gtagcaaaag
     5401 tggaagagca tattgccgat gcgctggaga aaggtgcgcg tgtggtttgc ggcggtaaag
     5461 cggatgaacg cggcggcaac ttcttccagc cgaccattct ggtggacgtg ccggccaacg
     5521 ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc gctgttccgc tttaaagatg
     5581 aagccgatgt gattgcgcaa gccaatgaca ccgagtttgg ccttgccgcc tatttctacg
     5641 cccgtgattt aagccgcgtc ttccgcgtgg gcgaagcgct ggagtacggc atcgtcggca
     5701 tcaataccgg cattatttcc aatgaagtgg ccccgttcgg cggcatcaaa gcctcgggtc
     5761 tgggtcgtga aggttcgaag tatggcatcg aagattactt agaaatcaaa tatatgtgca
     5821 tcggtcttta actggagaat gcgaatgaac agcaataaag agttaatgca gcgccgcagt
     5881 caggcgattc cccgtggcgt tgggcaaatt cacccgattt tcgctgaccg cgcggaaaac
     5941 tgccgggtgt gggacgttga aggccgtgag tatcttgatt tcgcgggcgg gattgcggtg
     6001 ctcaataccg ggcacctgca tccgaaggtg gtggccgcag tggaagcgca gttgaaaaaa
     6061 ctgtcgcaca cctgcttcca ggtgctggct tacgagccgt atctggagct gtgcgagatt
     6121 atgaatcaga aggtgccggg cgatttcgcc aagaaaacgc tgctggttac gaccggttcc
     6181 gaagcggtgg aaaacgcggt aaaaatcgcc cgcgccgcca ccaaacgtag cggcaccatc
     6241 gcttttagcg gcgcgtatca cgggcgcacg cattacacgc tggcgctgac cggcaaggtg
     6301 aatccgtact ctgcgggcat ggggctgatg ccgggtcatg tttatcgcgc gctttatcct
     6361 tgcccgctgc acggcataag cgaggatgac gctatcgcca gcatccaccg gatcttcaaa
     6421 aatgatgccg cgccggaaga tatcgccgcc atcgtgattg agccggttca gggcgaaggc
     6481 ggtttctacg ccgcgtcgcc agcctttatg cagcgtttac gcgctctgtg tgacgagcac
     6541 gggatcatgc tgattgccga tgaagtgcag agcggcgcgg ggcgtaccgg cacgctgttt
     6601 gcgatggagc agatgggcgt ggcgccggat cttaccacct ttgcgaaatc gatcgcaggc
     6661 ggtttcccgc tggcgggcgt caccgggcgc gcggaagtga tggacgctgt tgcaccgggc
     6721 ggtctgggcg gcacctatgc cggtaacccg attgcctgcg tggctgcgct ggaagtgttg
     6781 aaggtgttcg agcatgaaaa tctgctgcaa aaagccaacg atctggggca gaagctaaaa
     6841 gacggattgc tggcgatagc cgaaaaacac ccggagatcg gcgacgtacg cgggctggga
     6901 gcgatgattg ccatcgagct gtttgaagat ggcgatcaca gcaaaccgga cgcaaaactc
     6961 accgccgaga tcgtggcacg cgcccgcgat aagggcctga ttcttctctc ctgcggcccg
     7021 tattacaacg tgctgcgcat ccttgtaccg ctcaccattg aagacgctca gatccgtcag
     7081 ggtctggaga tcatcagcca gtgttttgct gaggcgaagc agtagctatg tccgtttgta
     7141 agagttaacc gttgctaagg gaatgataac gatgcagtaa aaaataataa cgatgaggcg
     7201 aacgacaaag ttaaaaacga ctgcggacag tcccctcgcc cctctggaga gagggttagg
     7261 gtgaggggta aaacacgcgg ctaaaccctc ccctggccct ctccctaagg gagagggaga
     7321 aaaccctccc gctttcgttt ttaagatttt gttgaccgtc tcaattaaac gcgtgacccg
     7381 gcgcgggaaa tgtcggggcg ctctcccaag tgacacactt tcgagaggat tcaggatggg
     7441 gcaatcatcg caaccacatg agttaggcgg cgggctgaaa tcacgccacg tcaccatgtt
     7501 gtctattgcc ggtgttatcg gcgcaagtct gtttgtcggt tccagcgtcg ccatcgccga
     7561 agcgggcccg gcggtattac tggcctatct gttcgccggg ctactggtgg ttatgattat
     7621 gcggatgttg gcggaaatgg cggttgccac gcccgatacc ggttcgtttt ccacctatgc
     7681 cgataaagcc attggtcgct gggcgggcta taccatcggc tggttgtact ggtggttttg
     7741 ggtactggtt atcccgctgg aagccaatat cgccgctatg atcctgcact cgtgggttcc
     7801 aggcattccc atctggttat tttccctcgt cattaccctt gccttaactg gcagtaattt
     7861 attaagcgtt aaaaactacg gcgaatttga gttctggctg gcgctgtgca aagtcatcgc
     7921 tatcctggcc tttattttcc ttggtgcagt cgcaattagc ggtttttacc cttatgccga
     7981 agtgagcggg atctcaagat tgtgggatag cggcggcttt atgcccaacg gtttcggtgc
     8041 ggtattaagc gcgatgttga tcaccatgtt ctcgtttatg ggcgcagaaa ttgtcaccat
     8101 tgccgccgcg gaatccgaca cgccggaaaa acatattgtc cgcgccacca actcggttat
     8161 ctggcgtatt tctatcttct atttgtgctc tatttttgtc gtcgtggcat taataccgtg
     8221 gaatatgccg gggctgaaag ccgttggatc ttatcgctcg gttctggaat tgctcaatat
     8281 tccccatgcg aaattaatca tggactgtgt gatattactt tccgtaacca gttgcctgaa
     8341 ctcggccctg tataccgcct caaggatgct ctactcctta agccgtcgcg gtgatgctcc
     8401 cgcggtaatg ggtaaaatca accgcagtaa aaccccgtac gtggcggtgt tactctccac
     8461 cggcgcggca ttcttaacgg tggtggtgaa ctattacgcg cctgcgaagg tatttaaatt
     8521 tctgatcgac agctccggcg ctatcgccct gctggtttat ttagtcatcg ccgtttcaca
     8581 gttgcggatg cgcaaaattc tgcgagcgga aggaagcgaa attcgcttgc gaatgtggct
     8641 ttatccctgg ctcacctggc tggtcatcgg ctttattacc tttgtgttgg tagtgatgct
     8701 attccgtccg gcgcaacagt tagaagtgat ctccaccggc ttattagcga tagggattat
     8761 ctgtaccgtg ccaattatgg ctcgctggaa aaagctggta ttgtggcaaa aaacacccgt
     8821 tcataatacg cgctgataat gatcaggagt cacaccatga ccattacgtc tctggatggc
     8881 tatcgctggc tgaagaacga tattattcgc ggtaattttc aaccggatga aaaattacgg
     8941 atgagtttgc tgacatcgcg ttatgcactt ggcgttgggc cgttgcggga agcactttct
     9001 caactggtgg cggaacggct ggtcacggtg gtgaatcaaa aagggtatcg ggtggcgtct
     9061 atgtcagagc aggagctgct cgatattttc gatgcccgcg ccaatatgga agcgatgtta
     9121 gtgagtctgg cgattgcccg cggtggcgat gagtgggagg cagacgttct cgcaaaagcg
     9181 catctgctca gtaagctgga agcctgtgac gccagcgaga aaatgcttga tgagtgggat
     9241 ctgcgtcatc aggcgtttca tacagcaatt gtggcgggct gtggttctca ctatttgctg
     9301 caaatgcgtg aacggttgtt tgatctggcg gcgcgctatc gatttatctg gctgcggcga
     9361 acggtgcttt cggtagaaat gctggaggat aaacacgatc agcaccagac cctgactgcg
     9421 gcggtactgg cgcgagatac cgcgcgcgcc agtgagttaa tgcgccagca tttactgacg
     9481 ccaattccca ttatccagca ggcgatggct ggcaattaac tactcttccg gaatacgcaa
     9541 cacttgcccc ggataaattt tatccgggct ttttagcatc ggtttattcg cttcgaagat
     9601 tttattgtac agattagcgt taccgtagac ctgtttggaa atggcactca gagtgtcgcc
     9661 agacttaacg gtataaaact ggctggcagt ggctggtgtc gccgttttca cctgatcatc
     9721 aacactggca ataccggaaa tattccccac cgcaacaagg attttctcct tcgcctcctg
     9781 actcaggccg tcaccagtga ccgtcgcttt gccgtcggca atttgaatgt tcactttatc
     9841 agcatcaggt ataccggttt tgctcagatg ctcctgcacc ttcttcgcct gatcgtcttt
     9901 atcgtgctga cctgtaaccg cgtcccagag tttttctccg gcatctttca caaaattgaa
     9961 cagacccata gctacctcat tcgttaacgg aaacactcag aaagtgtagc agaggaacgc
    10021 ggcgcgttaa cgccgcgggg gtgagcacag gtttaatcgc gggtttgcac ccagaacgcg
    10081 tgaatcaaac cggggatata cccaagcagt gtcagcagaa tattaataat gaacgcccaa
    10141 ccgaaccctt taccgagcag cacgccgagc ggcggtagaa tgatggtgat gacgattctc
    10201 cagaaaccca tatgtactcc ctataagaaa attactcatt gtttaaaaag agattttatc
    10261 tcttaagcgt agtaatttta gcggagcctg ccagtttcag cttacgcttt tactctcatt
    10321 tacgctgctg aggctggtat atgttgcatt tttagtttag agcttactta atttagaaaa
    10381 tacttaaata aatatgactg aactcgcgca attacaggcc agtgccgaac aggcagcggc
    10441 cttattgaaa gcaatgagcc atcctaaacg attgctgatt ctgtgcatgc ttagcggttc
    10501 ccccggcacc agcgcgggag agctgacgcg cattaccgga ctgagtgcct ctgcgacatc
    10561 acagcatctc gctcgtatgc gggacgaagg gcttatcgac agccaacggg atgcccaacg
    10621 cattctatat tccattaaaa atgaggcggt aaatgccatt atcgccaccc tgaaaaatgt
    10681 ctattgtccg taaggagtca ctatggcttt gacaaccatt tcgccgcatg atgcacaaga
    10741 attaatcgca cgcggcgcaa agttaatcga tattcgtgac gctgatgaat atcttcgtga
    10801 acatattcct gaagcagatc tggctccatt atccgtgctg gaacagtcag gtcttccacc
    10861 taaattacgt cgcgagcaaa ttatattcca ctgccaggca ggtaaacgca ccagtaataa
    10921 cgccgataaa ttagcagcga ttgccgcccc cgcagaaata tttttactcg aagatgggat
    10981 tgatggctgg aaaagagctg gattgccagt agcggtaaat aaatctcaac ccttgccgtt
    11041 aatgcgccag gtgcagatcg ctgcgggtgg tttaatatta atcggcgttg tactgggtta
    11101 taccgtaaat agcggtttct tcttattaag tggctttgtt ggtgccgggt tactgtttgc
    11161 aggaatcagc ggtttttgtg gaatggcaag gttgttagat aagatgccgt ggaaccaacg
    11221 agcttgagaa gcgacgccgg acgcgcccta gcagcgacat ccggcctcag taattagatc
    11281 aggaaatcgt cgagagattt accttctgcc agcgcctgag caattggctt cggtgtacgg
    11341 ccctgaccgg tccaggtttt agtttcaccg ttaacatcgg tgaatttata tttcgccgga
    11401 cgcggctggc gttttttacc agcgcgtggt gcagcagcag agctattacc caataactct
    11461 tccgggttaa ttccgtcagc cttcatcagc tccagccagg tgctaatttt ttcctgacgc
    11521 tcagccagtt cacgctgctg ctgttcttct tcttcacgtc tttctttagt gacaaccctg
    11581 aatttttcga gcatttcttc aagaacgtca atggagaatt cgcgagccat cgcacggagg
    11641 gtgcgaatgt tatttaaact ttgtaacatt acggacataa taaagaaaac cttttaacgc
    11701 caaaacaaaa aagtatttcg tcgcgtagtg taagcgatca cttattatat ttccacaaaa
    11761 tttaatctat ttacagtctt gcgcgagatt atttcagctg ttattttctg aaactctcag
    11821 aataatttca agattatata ttagccattt tgatgagtaa tttagattat tggtgaacag
    11881 aacggcgctc tctgctcgct aattccgcaa gcctgacaaa accgaagtga ataaaaatgc
    11941 aatataacag aataaaaga
//
LOCUS       DAZPJR010000125        11914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPJR010000125 DAZPJR010000000
VERSION     DAZPJR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.126"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            32..598
                     /locus_tag="ACHVB7_003141"
     CDS             32..598
                     /locus_tag="ACHVB7_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1915054.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            754..2786
                     /locus_tag="ACHVB7_003142"
                     /pseudo
     CDS             754..2786
                     /locus_tag="ACHVB7_003142"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2763..3107
                     /locus_tag="ACHVB7_003143"
     CDS             2763..3107
                     /locus_tag="ACHVB7_003143"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR1915055.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3122..3853
                     /locus_tag="ACHVB7_003144"
     CDS             3122..3853
                     /locus_tag="ACHVB7_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1915056.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3850..4911
                     /locus_tag="ACHVB7_003145"
     CDS             3850..4911
                     /locus_tag="ACHVB7_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1915057.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5063..6109
                     /locus_tag="ACHVB7_003146"
     CDS             5063..6109
                     /locus_tag="ACHVB7_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR1915058.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6106..6897)
                     /gene="nei"
                     /locus_tag="ACHVB7_003147"
     CDS             complement(6106..6897)
                     /gene="nei"
                     /locus_tag="ACHVB7_003147"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR1915059.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6933..7667)
                     /gene="pxpA"
                     /locus_tag="ACHVB7_003148"
     CDS             complement(6933..7667)
                     /gene="pxpA"
                     /locus_tag="ACHVB7_003148"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR1915060.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7657..8589)
                     /gene="pxpC"
                     /locus_tag="ACHVB7_003149"
     CDS             complement(7657..8589)
                     /gene="pxpC"
                     /locus_tag="ACHVB7_003149"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR1915061.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8583..9239)
                     /gene="pxpB"
                     /locus_tag="ACHVB7_003150"
     CDS             complement(8583..9239)
                     /gene="pxpB"
                     /locus_tag="ACHVB7_003150"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR1915062.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9262..10005)
                     /gene="ybgI"
                     /locus_tag="ACHVB7_003151"
     CDS             complement(9262..10005)
                     /gene="ybgI"
                     /locus_tag="ACHVB7_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR1915063.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10276..11757
                     /gene="dtpD"
                     /locus_tag="ACHVB7_003152"
     CDS             10276..11757
                     /gene="dtpD"
                     /locus_tag="ACHVB7_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR1915064.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2836 a         3179 c         2929 g         2970 t
ORIGIN      
        1 acttttttct tttctgttaa aggactcagt tatgtttaaa ggacaaaaaa cattggccgc
       61 actggccgta tctctgctgt tcacagcacc tgtttatgcg gctgatgaag gatccggtga
      121 aattcacttt aaaggtgaag ttattgaagc accgtgtgaa atacatcagg atgatattga
      181 taaagaggtt gaactcggtc aggtgaccac cagccacatt aatcagtcac atcacagcga
      241 tgccgttgct gtcgacctgc gcttagtcaa ctgtgatctg gaaaactcca gcaacggttc
      301 cggtggcaag atttctaaag ttgcagtcac ctttgatagc tcagcgaaaa ccaccggcgc
      361 agatccgatt ctcaacaaca ccagcacggg tgaagccacc ggcgtcggcg tacgtttaat
      421 gaataaagat caaagcaata tcgttttagg caccgctact ccagatatcg acctggctcc
      481 gacctccagc gaacaaacgc tgaatttctt tgcctggatg gaacaaattg atcaggctac
      541 acctgtaacg ccaggcgcag ttacggcgaa tgcaacctac gtgctggatt ataaataagt
      601 attactttgg cggcgctatc gccgcctttc ataaaatatt tatcaggtat ggacaccgtg
      661 aatatttatc gactctcttt tgtatcctgc ctggtcgtgg cgatgccttg cgcattggcg
      721 gtcgaattca accttaatgt tctcgataaa tcgatgcgcg accgcattga tatttcatta
      781 ttaaaggaaa aaggagtcat tgctcccggt gagtattttg ttagcgttgc ggtaaataat
      841 aaccaaatca gtaacgggca aaagattaac tggcacaaaa atgacgataa aaccattccg
      901 tgcatcaatg atttactggt cgataaattt ggcttaaaac ctgaagtccg tcagtcgtta
      961 ccattgataa atcagtgcgt cgattttagc tcccgacctg aaatgctctt caatttcgat
     1021 caagccaatc agcaactgaa tattagtatt ccgcaagcct ggctggcgtg gcactcagaa
     1081 aactggaccc caccctccac atggaaagaa ggtgtcgccg gtatcctgat ggattacaac
     1141 ttgtttgcca gcagctaccg cccacaggac ggcagcagca gcactaacct gaacgcctac
     1201 ggtaccaccg gaattaacgc cggggcatgg cgcttacgta gtgattatca gttgaatcag
     1261 actgatagcg atgataacca tgaacagtca ggcggaatat cgcgcaccta tctttttcgt
     1321 ccattaccgc aattaggctc taaattaacc ctcggcgaaa cggattttag ttccaatatt
     1381 ttcgacggtt tttcttatac cggcgcggca ctggcaagtg acgagcgaat gttgccatgg
     1441 gaactacgcg gctacgcccc acaaattagc ggtattgcac agaccaatgc cacggtgacg
     1501 atcagtcaat caggccgcgt catttaccag aaaaaagtcc caccaggccc atttatcatt
     1561 gacgacctta atcagtctgt tcagggcaca ctggatgtca aagtgacgga agaagatggt
     1621 cgggtgaaca atttccaggt ttcggcagca tcgacgccct tcctgactcg tcagggacag
     1681 gttcgctata aactggccgc gggtcagcca cggccctcca tgtcacatca aactgaaaat
     1741 gaaacctttt ttagcaatga agtttcctgg gggatgctgt caaacacctc gctgtacggc
     1801 ggcctgctgc tttctggtga tgactaccat tctgccgcaa tgggtattgg gcaaaatatg
     1861 ctgtggcttg gtgcgctgtc gtttgatgtc acgtgggcca gtagccattt tgatactcag
     1921 caggacgagc ggggcttaag ctaccgtttt aattacagca aacaagtgga tgccactaac
     1981 agcacgattt ccctcgccgc ttatcgtttc tccgatcgtc attttcacag ctacgccaac
     2041 tatctggatc acaaatacaa cgacagcgat gcgcaggacg aaaaacagac gatcagctta
     2101 ttgtgggcca accgattacc ccactaaacc tcaatcttta cgccaacctg ctacatcaaa
     2161 cctggtggaa tgcagacgcc tccacgaccg ccaacatcac ggcaggtttt aatgttgata
     2221 ttggtgactg gagagatatc tctatttcga cgtcattcaa tacaacccat tacgaagata
     2281 aagatcacga caaccagatt tacctgtcga tttcgctccc cttcggtaac ggtggtcggg
     2341 ttggctatga catgcaaaac agtagccaca gcaccacaca ccgcatgtcg tggaacgata
     2401 cgctggatga acgtaatagc tggggcatgt ctgccggact gcaatccgac cgtcctgaca
     2461 atggagccca ggtgagcggt aactatcagc acctgagttc agcgggtgag tgggatattt
     2521 ctggtaccta tgccgccaat gattacagtt ccgtcagcag cagctggagc ggttctttca
     2581 ccgcaaccca atatggtgca gcgtttcatc gccgcagctc caccaatgaa ccacgcctga
     2641 tggtcagcac cgatggcgtg gcagatattc cggttcaggg caatctcgac tacaccaacc
     2701 attttggcat tgcggtggtg ccgttgattt ccagttacca gccttccacc gtggcggtga
     2761 acatgaatga cttacccgac ggcgtaacag ttgcagaaaa cgttatcaaa gaaacgtgga
     2821 ttgaaggcgc gataggttac aaatcactgg cttcccgttc cggtaaagac gttaacgtca
     2881 tcattcgcaa cgccagcggt cagtttcctc ccctcggagc ggatatccgc caggatgaca
     2941 gcggcattag cgtggggatg gttggcgagg aaggacatgc ctggttaagc ggagtcgctg
     3001 aaaatcaaaa gtttaccgtg gtctggggtg atagccagca ttgctcgctc catcttcctg
     3061 aacatatgga agacaccgca aatcgcctga ttttaccttg tcattaatta aggaaatgaa
     3121 aatgacattt atgaaaggac tacccctttt gttgttggtt gccagtctgt gcagccacgc
     3181 tgcactacaa cccgatcgca ctcgtattgt gtttaacgct aatgataaag ctaccagtct
     3241 gcgggtggat aatcgtagcg ataaactccc ctacctcgct tattcctggc tcgaaaatga
     3301 aaaaggggag aaaagcgatg atcttctggt cgccctgccg ccgatacaac gtctggaacc
     3361 gaaagcaaca acgcaggtac ggatagtaaa gcaagcctcg accacaaaat taccgggcga
     3421 tcgcgaaacg ctatttttct acaatatgcg tgaaattcct cccgccccgg aaaaaaacag
     3481 cgaccatgcc gttctgcaag tcgccattca gagtcgtatc aaggtgttct ggcgaccggc
     3541 tgcattacgc aagaaagcag gagaaaaggt cgaactgcag ttacaggtca gccagcaggg
     3601 caaccaactg acactgaaaa atcctaccgc gtattatctg accattgcct acctgggacg
     3661 aaatgaaaaa ggcgttctcc ccggcttcaa aaccgtgatg gttgcgcctt ttagtacggt
     3721 caacacaaac accggaaatt atagcggcag ccagttttat ctcggttata tggatgatta
     3781 cggcgcgtta cgcatgacga cgctcaactg cagcggacaa tgccatttac aagccgtgga
     3841 ggcgaagaaa tgagtgctgg caagggagtg ttgctcgtca tctgtctgtt atttctgccg
     3901 ttgaagtctg cgcttgccct gaactgctac tttggttcat ccggtggctc ggtagaaaaa
     3961 tcagaagcga ttcaaccttt tgctgtacca ggcaatgcca aactcggcga taagatttgg
     4021 gaatctgacg atattaaaat tcccgtctat tgtgacaaca ataccaacgg caattttgaa
     4081 agtgagcatg tttatgcctg ggtaaacccc tacccggggg tgcaggatcg ctattatcaa
     4141 ctcggcgtaa cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac
     4201 actaaccagt gtattgacag taagaacatt gacatttata cccctgagca gattatcgcg
     4261 atgggatggc agaacaaaat ttgctcgggt gatcccgcta atattcacat gtcgcgtacc
     4321 tttctcgctc gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca tgattatcaa
     4381 agtacgctta gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt taacgaagac
     4441 cctactgccc aaaacctgaa atatcatatt actggtctgg aaaacattcg cgtgctggat
     4501 tgcagcgtca atttttccat ttcaccggaa acacaggtga ttgattttgg taaatttaat
     4561 ttgctggata ttcgtcgcca cacaatgtcg aaaacattca gtattaagac gacaaaatca
     4621 caaaatgacc aatgcaccga cggatttaaa gtcagttcct ccttttatac cgaagaaacg
     4681 ttggttgaag aagataaagc attgttgatt ggtaatggtc tgaagctacg tttgctggat
     4741 gaaaacgcct cgccttatac cttcaataaa tatgctgaat acgccgattt caccagcgac
     4801 atgttggtct acgaaaaaac ctatacagct gaactttcgt ccattgcagg tacccccatc
     4861 gaggctgggc ccttcgatac agtggtgctt tttaaaatta actataactg atttgccatg
     4921 atgccctttg cagccggaaa gggcatctct ttcagaaaat tgctgatttt ccccttttcc
     4981 ttgattgctg ttattttttc ctggcccggc tttctttatg ctgtatcgat acaccaaaga
     5041 gaaaataatg agggagcgaa ggatgccagt tttgcagtgg ggaatgttat gtgtgctatc
     5101 acttctcctt tctattggtt tcctcgcggt acatctcccg gcggcgctat tacttgggcc
     5161 gatgatcgcc gggatcatct tcagtatgcg cggaattact ctgcaacttc cccgctccgc
     5221 ttttctcgcc gcgcaggcca ttcttggctg catgattgcg caaaacctca ctggttcgat
     5281 tctcaccacg ctggccgcca actggccgat cgtgcttgct attttactgg tgacgctgct
     5341 ttccagcgcc atcgtgggtt ggttattggt gcgctatagc tcactgcctg gaaataccgg
     5401 tgcctggggt tcttctccag gcggcgcagc ggcaatggtc gctatggcgc aagattacgg
     5461 cgcagatatt cgcctggttg catttatgca atatctgcga gtgctgttcg tcgcgggggc
     5521 cgctgttctg gtcacccgca tgatgctggg tgataacgct gaagcggtta accagcaaat
     5581 cgtctggttc ccgccagtga gcattaatct cctgcttacg attttgctcg cagtcgttgc
     5641 cggtacggcg ggatgcatgc tgcgtcttcc ttccggcacg atgctcatcc cgatgctggc
     5701 gggcgcagtg ctccagtctg gtcagctcat caccatcgaa ctaccggaat ggctgctggc
     5761 gatggcgtat atggcaattg gctggcggat tggtcttggt ttcgataaac aaatcttact
     5821 gcgggcacta cgcccgctac cgcaaatcct gctgtcgatt tttgctctgc tggctatttg
     5881 tgcgggtatg gcgtgggggc tgacccggtt tatgcacatt gattttatga ctgcctacct
     5941 cgccaccagt cccggcgggc ttgataccgt ggcggtcatc gccgcaggga gcaatgccga
     6001 tatggcgctt attatggcga tgcaaaccct gcgcctgttc agtattttgc tgacggggcc
     6061 agccattgcg cggtttattt caacctatgc gccgaagcgg tcggcctagt gctggcagcc
     6121 agggcaccag taaaacgggc gagatgacag cgtggttttc tcaatgatgc tgccacagcg
     6181 ttcgcacggt tcgccatcac gatgaaaaac cttaaagcga aacagcgccc catgatgctt
     6241 attctcatcc accagccccc tcgtagcgta ggaaaatcga ggaatatcca gtaacgcgtg
     6301 tgcgagtgca tccagttgcg ccgcattgag atctttcgct ttatgatttc cagtcaaccc
     6361 aacctgccag aggatctcca cccgcaaata attgccaagc ccagccagaa acgcctgatc
     6421 gagcagtaat cctgcaaact gacggttacg aaagcgcggt gacaataacc gttcctttac
     6481 cacctccggc gtcagattcg gatccagcac atcgggacca acgcgttgta aaaacggatg
     6541 cgtggtcagt tgttccggcc tcaacatctc aatatccgag gcgctataaa gcagaatggt
     6601 tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg gtctgcggct cttcgccggt
     6661 atcaaccacg cgccagacgc cgtaaagctg attatggctg tagagcgtta agtcgttgga
     6721 aaaatgagtt aacaacgcct taccacgcgt ttccacatgg gtaacgtgtt gaccgataag
     6781 ttgtgattga taagttttta actgcgggaa ggcaaaccag acatcagtta gtggtttgcc
     6841 tttgatcgcc gcctccaggt tatccgctgc acggcggatc tccgggcctt caggcatggt
     6901 gatatccttt tttcattctt tattaatcgg ggttatgctg cgacaacaat ctccttttcg
     6961 gcaaatgtag agcgtagtcg gcgggcgaac gccagtgcgt gctcgccgtc gccatgcagg
     7021 cagactgttt gcgccgtgac cgttgcccat tcgccggtga tacttttgac tctgccgtgt
     7081 tgcaccatct ctagcgtttg cgccaacgcc tgttcttcgt tttcaatcaa cgcgcctcgc
     7141 tgacttcgcg gcaccagcga gccgtcagcc tgataaccgc gatcggcaaa cacttcctcg
     7201 cgcgttgtca ggccatattg cttgcctgca cgaatcagct cgcttcctgc cagcccaacg
     7261 agaatcaatg ccggatcgca agcgtatacc gctctggcga tggcgtctgc cagttgtgct
     7321 tctttcgccg cctggttgta caacatgccg tgcggtttga catgacgcat tacgccgcct
     7381 tgcgcacggg caatcgttgc cagcgcgcca atttgataca gcgtctgggc gtaaacggtt
     7441 tctggcggca gctgcatggc gctgcgacca aaattttccc tgtcgggaaa actcgggtga
     7501 gcgccaatcg cgacaccatt ttttattgct tcacgcacgc aagcctgcat gatttgcgca
     7561 tcgcctgcat gaaatccaca ggcaatattg gcagaggaaa ccagcgttaa tagctctgcg
     7621 tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa ttttcattgt gcagccgcca
     7681 cactaattgt tcgaaataac gttgctgatc ttgccgcgct ttcagtgcct cttccagtga
     7741 acactggaca aaatgaatcg gctgaccgag cggaatttgc gccagatggt acatatcagc
     7801 ctcaatgata caggcaatac gcgggtaacc atcggtggtc tgtgcgtcgt tcatcaacac
     7861 aatcggctgc ccgttatgtg gcacctgcac cacgcccggt aacaaaccgt gagataacag
     7921 ttcgcgatcg gtggtgcgtt ttaaaatttg cccctgtaag cgatagccca tgcggttact
     7981 ttgcgagcta agctgccagg gcgaacgcca gaatgcatcc tgcgaggcgc gatcgaactc
     8041 atgatattcc ggccccggca aggcgcgaat gcggttgccc cacagcagct gtttaacgcc
     8101 ctgcgcttcc ataaaatcac gcttcgattt gccaatcggg agtcggtcac catccttcag
     8161 taaacggcct tccagcccgc caatccccac tttgagatcg gtgctgcatg accccattac
     8221 cggcggaaca tcaataccac ccgcgaccgc cagataactg cgcatcccgt gctgcgggcg
     8281 tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc cagccggtcc agacggcatt
     8341 atcatccagc cgcgcttcgc aaccggcacc cgtcagagca aaccacccat cagtttcgaa
     8401 ctcaaccgtt aactgaccga gcgtgatctc cagcgcgggg gcattggcgt cattacccac
     8461 cagcaggtta gcaatgcgta acgcaggcat atccagtgcg ccgcagtggc tgattcccga
     8521 ctggcgaaaa ccgtgacgac cgccatcctg cacagtggta tacatgcccg cacgaataat
     8581 cttcagcata ctccctcctt ctgcggtaca aagcgcacgc tgtctcccgg acgtaataag
     8641 atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg tatgaccaat caactgccag
     8701 ccacccggcg ttgccagcgg ataaacacca gtctgcggcc cgccgatccc gacagaacct
     8761 gccggaacga gcaagcgcgg ttcagcgcgc cgtggcgtgt gtagttgttc cggcaaactc
     8821 ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga ccacgtattc cacggaggag
     8881 tgcaattcaa caacctgttt ttcgctaaac ccgcaatgcg ccgcgaccac cgccaaatcc
     8941 ggtccgcctg caccaccgta aaccaccgga atttcaataa agcgagactc aggttccagg
     9001 gcctcgctct cctcccacca gcgttgcaaa cgctctatgg catccagcgc cagcgactca
     9061 ggattacgca aaatcaccgt gatattgttc atgccgggaa tggcttcaac cacattcggc
     9121 atatccacca gacgctgcgc cagtcgccag atccgtttct ggctagccag cgtcaccggc
     9181 ggttccagtt ccagcactac cgccgtttca cctatcagat aacaacgcgc tcgttgcact
     9241 ttcgatccct ctgattattc gttatgcagg attaggaata tcaataaagg tcacatcaag
     9301 atcggtattt tcattcagcc actcgctcaa tgcgcgaata ccaccacgtt cagtggcatg
     9361 gtgacctgca gcataaaaat gcaacccctg ctcgcgggct gaatgaatgg tctgttcaga
     9421 aacttcgcca gtaataaaag catccacgcc aaaacgcgcg gcgctatcga taaaactttg
     9481 cccgccgccc gtgcaccagg cgacgcgctg gaccacctca ggtccggtat cgccacacca
     9541 taacggctta cgtcccagac gcgcttcaat ccaggaggct agttccagtc ccggcacagg
     9601 catggtcagc tctccccacg gcaccaacgg ctcaatttcg cccatgaccg tgatccccag
     9661 taacgccgcc agctgtgcgt tattgcccag ctcaggatgt acgtcaagcg gcaaatgcca
     9721 gccatacagg ttgatatcat tcgccagcaa cgtttttaaa cggttacgct tcattccgcg
     9781 aatgaccgga gactctcctt tccagaagta gccatgatgc acaatgaccg catccgcgcc
     9841 cagacgcact gcctcatcga gcaaagcctg gctggcggtg acaccggtaa caattttttg
     9901 caccgtctct ttgccttcta cctgcaaacc attcggcgcg taatcactaa tcgccgcgct
     9961 gttcagtttt tcgttaatca gttgttccag ttcggtgttt ttcatcttcg cgccttccgt
    10021 attctgatag ctacaccgtt aacatagcgt tacctcgcct gttcgtcgat agccaatctt
    10081 ttgacatatt aagaaacaaa cccgacgcca aaagacgcaa attgtttata tcatcacgct
    10141 tcagctaatg tatatttatg caatcagaca gcatattgca taaataatca gatctgtatc
    10201 aaagttttaa ctttaaggaa acacaagcac gccgcgcaac tattacgtga cgttgcagtc
    10261 aattgtggaa ttattatgaa taaacacgca tcacaaccgc gcactattta ctatgtcgtt
    10321 gcgctgcaaa tctgggaata ttttagcttt tacggcatgc gtgccctgct gattctctat
    10381 ctcaccaatc aactaaaata caacgatact cacgcctacg agttatttag cgcctactgt
    10441 tcgctggtgt atgtcacgcc aatcctcggt ggctttttgg cggataaagt tctcggcaat
    10501 cgcatggcgg tgatgctggg ggcgttgttg atggcgatcg gtcatgtggt gctgggtgcc
    10561 agtgagatcc atccgtcatt cctctatctg tccctggcga ttatcgtttg tggctatggt
    10621 ctgtttaaat ccaatgtcag ttgcctgctc ggcgagctgt atgagccaac cgatccgcgt
    10681 cgtgatggcg gtttctcgct gatgtatgca gcgggtaacg tggggtctat tatcgcacct
    10741 atcgcctgtg gttacgccca ggaagagtac agctgggcga tgggctttgg cctggcggcg
    10801 gtgggtatga tcgcgggtct ggtcattttc ttatgtggca atcgtcattt cacacatacc
    10861 cgcggcgtta ataaaaaagt actgcgtgca acaaactttc tcctgcctaa ctggggatgg
    10921 ctgctggttc tgctggtggc aacgcctgcg ctgattaccg tactgttctg gaaagagtgg
    10981 tcggtatacg ccttaattgt cgcgactatt attggcctgg gtgtactggc aaaaatttat
    11041 cgcaaagcag aaaaccagaa acagcgtaag gagctggggc tgattgtgac gctcaccttc
    11101 ttcagtatgt tgttctgggc cttcgcacaa cagggcggca gctcaattag cctttatatc
    11161 gaccgcttcg ttaaccgcga tatgtttggt tataccgttc cgactgctat gttccagtcg
    11221 attaatgcct tcgcagttat gctatgcggt gtgttcctgg cgtgggtggt taaagaaagc
    11281 gtcgcaggta atcgtaccgt gcgcatctgg ggaaaatttg ctcttggcct tggcctgatg
    11341 agcgccggat tctgcattct gaccttaagc gcccgctggt ccgcaatgta tggtcactct
    11401 tctctgccac tgatggtgtt aggcctcgcg gtgatgggct ttgcggaact gtttatcgac
    11461 ccggttgcca tgtcgcaaat tacgcgcatt gaaattccgg gcgtgaccgg cgtattaacc
    11521 ggcatctaca tgctgctttc tggcgcgatc gcgaactatc tggcgggcgt gattgccgat
    11581 cagacatcgc aggcttcgtt tgatgcttcc ggggcgatca actactccat caatgcatat
    11641 attgaagtat ttgatcaaat tacctggggc gcactggcgt gtgtaggact ggtactgatg
    11701 atttggctgt atcaggcgct gaaattcaga aaccgcgcgc tggcgctgga gtcataatag
    11761 gccagcaccg atgcctgatg cgacgcttga cgcgtcttat caggccggtg atgctgccaa
    11821 cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca
    11881 gctgtccctc ctgttcagct actgacgggg gggt
//
LOCUS       DAZPJR010000126        11816 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPJR010000126 DAZPJR010000000
VERSION     DAZPJR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.127"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHVB7_003153"
     CDS             <1..389
                     /locus_tag="ACHVB7_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR1915065.1"
                     /translation="SSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALL
                     KREGRCPSDVEHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRAL
                     RKGQASAFYYGDPLGEMRLVSRVFEM"
     gene            complement(588..938)
                     /locus_tag="ACHVB7_003154"
                     /pseudo
     CDS             complement(588..938)
                     /locus_tag="ACHVB7_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(1141..2154)
                     /gene="intI1"
                     /locus_tag="ACHVB7_003155"
     CDS             complement(1141..2154)
                     /gene="intI1"
                     /locus_tag="ACHVB7_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1915066.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2312..2785
                     /gene="dfrA17"
                     /locus_tag="ACHVB7_003156"
     CDS             2312..2785
                     /gene="dfrA17"
                     /locus_tag="ACHVB7_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR1915067.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            2916..3704
                     /locus_tag="ACHVB7_003157"
     CDS             2916..3704
                     /locus_tag="ACHVB7_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR1915068.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3910..4257
                     /locus_tag="ACHVB7_003158"
     CDS             3910..4257
                     /locus_tag="ACHVB7_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR1915069.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            4251..5090
                     /gene="sul1"
                     /locus_tag="ACHVB7_003159"
     CDS             4251..5090
                     /gene="sul1"
                     /locus_tag="ACHVB7_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR1915070.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            5218..5421
                     /locus_tag="ACHVB7_003160"
     CDS             5218..5421
                     /locus_tag="ACHVB7_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915071.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            5577..6782
                     /gene="chrA"
                     /locus_tag="ACHVB7_003161"
     CDS             5577..6782
                     /gene="chrA"
                     /locus_tag="ACHVB7_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR1915072.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            6793..7098
                     /locus_tag="ACHVB7_003162"
     CDS             6793..7098
                     /locus_tag="ACHVB7_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR1915073.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            7325..8089
                     /locus_tag="ACHVB7_003163"
     CDS             7325..8089
                     /locus_tag="ACHVB7_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR1915074.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(8582..9166)
                     /gene="mphR(A)"
                     /locus_tag="ACHVB7_003164"
     CDS             complement(8582..9166)
                     /gene="mphR(A)"
                     /locus_tag="ACHVB7_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR1915075.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(9166..10404)
                     /gene="mrx(A)"
                     /locus_tag="ACHVB7_003165"
                     /gene_synonym="mrx"
     CDS             complement(9166..10404)
                     /gene="mrx(A)"
                     /locus_tag="ACHVB7_003165"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR1915076.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(10401..11306)
                     /locus_tag="ACHVB7_003166"
     CDS             complement(10401..11306)
                     /locus_tag="ACHVB7_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR1915077.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(11428..>11816)
                     /locus_tag="ACHVB7_003167"
     CDS             complement(11428..>11816)
                     /locus_tag="ACHVB7_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR1915078.1"
                     /translation="SSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALL
                     KREGRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRAL
                     RKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT         2490 a         3391 c         3450 g         2485 t
ORIGIN      
        1 tctcctcccg tcgtaacagc aaagctgcat accggtttct gggtaaaatc ctcaacaacg
       61 tgaagaagtg gcagatccca cgattcatca acacggataa agcgcccgcc tatggtcgcg
      121 cgcttgctct gctcaaacgc gaaggccggt gcccgtctga cgttgaacac cgacagatta
      181 agtaccggaa caacgtgatt gaatgcgatc atggcaaact gaaacggata atcaacgcca
      241 cgctgggatt taaatccatg aagacggctt acgccaccat caaaggtatt gaggtgatgc
      301 gtgcactacg caaaggccag gcctcagcat tttattatgg tgatcccctg ggcgaaatgc
      361 gcctggtaag cagagttttt gaaatgtaag gcctttgaat aagacaaaag gctgcctcat
      421 cgctaacttt gcaacagtgc ccccaatgcg ctgtccagtc atggaattcc ctgcaaaatg
      481 tcagggaaga ctctatgacc ttcaacgaga tatgtcaata aattcaaaat tcaatcctat
      541 cctgacgcaa tttacacatg gcatctgaca tcaggttagg gtatgcctca acctgacggc
      601 ggcgaatcac aagcgtccgg tttgacgctg gtttcggtcg cagtgctggc ccgcgcctgg
      661 aagcgctgat agcactccag cccgcagaaa tgctccacgt actcggcccc ttccggcgtg
      721 aaggcggcat cgagcgggat ttccttgcag cacacgcagc aactggtgct cggttcattg
      781 gcgttcatgg tggtgttcct ccattggttg acgaagccga cgaaggccgc cgccggcatc
      841 ggcctggcga acaggaaacc ctgcaccgtg tcgcaacccg cctgccgcaa ccacgcaagg
      901 cagtcgggtg tttccacacc ctcggctacc acctccattg tcgttttcag aagacggctg
      961 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatcc atcaggcaac
     1021 gacgggctgc tgccggccat cagcggacgc agggaggact ttccgcaacc ggccgttcga
     1081 tgcggcaccg atggccttcg cgcaggggta gtgaatccgc caggattgac ttgcgctgcc
     1141 ctacctctca ctagtgaggg gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc
     1201 aactttcagc acatgcgtgt aaatcatcgt cgtagagacg tcggaatggc cgagcagatc
     1261 ctgcacggtt cgaatgtcgt aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag
     1321 ggtgtgcggt gtggcgggct tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg
     1381 ctgaaaggtc tggtcataca tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga
     1441 atgcgtgtgc tgcgcaaaaa cccagaacca cggccaggaa tgcccggcgc gcggatactt
     1501 ccgctcaagg gcgtcgggaa gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca
     1561 ccatgcccgt gcacgcgaca gctgctcgcg caggctgggt gccaagctct cgggtaacat
     1621 caaggcccga tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc
     1681 cagatccttg acccgcagtt gcaaaccctc actgatccgc atgcccgttc catacagaag
     1741 ctgggcgaac aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg
     1801 ggtcagcacc accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg
     1861 cagatccgtg cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg
     1921 cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct
     1981 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     2041 gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct cacgcaactg
     2101 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     2161 ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     2221 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     2281 taaaacaaag ttagccatta agggagttaa attgaaaata tcattgattt ctgcagtgtc
     2341 agaaaatggc gtaatcggta gtggtcctga tatcccgtgg tcagtaaaag gtgagcaact
     2401 actctttaaa gcgctcacat ataatcaatg gctccttgtc ggaagaaaaa catttgactc
     2461 tatgggtgtt cttccaaatc gcaaatatgc agtagtgtca aagaacggaa tttcaagctc
     2521 aaatgaaaac gtcctagttt ttccttcaat agaaaatgct ttgaaagagc tatcaaaagt
     2581 tacagatcat gtatatgtct ctggcggggg tcaaatctat aatagcctta ttgaaaaagc
     2641 agatataatt catttgtcta ctgttcacgt tgaagtcgaa ggtgatatca aattccctat
     2701 aatgcctgag aatttcaatt tggtttttga acagtttttt atgtctaata taaattatac
     2761 ataccagatt tggaaaaaag gctaacaatg cgttgcagca ccagtcgctt cgctccttgg
     2821 acagctttta agtcgcgtct ttgtggtttt gctgcgcaaa agtattccac aaagccgcaa
     2881 cttaaaagct gccgctgaac ttaacgttag gcatcatggg tgaatttttc cctgcacaag
     2941 ttttcaagca gctgtcccac gctcgcgcgg tgatcgagcg ccatctggct gcgacactgg
     3001 acacaatcca cctgttcgga tctgcgatcg atggagggct gaagccggac agcgacatag
     3061 acttgctcgt gaccgtcagc gccgcaccta acgattcgct ccggcaggcg ctaatgctcg
     3121 atttgctgaa agtctcatca ccgccaggcg atggcggaac atggcgaccg ctggagctaa
     3181 ctgttgtcgc tcgaagcgaa gtagtgcctt ggcgctatcc ggcgcggcgt gagcttcagt
     3241 tcggtgagtg gctccgccac gacatccttt ccggaacgtt cgagcctgcc gttctggatc
     3301 acgatcttgc gattttgctg accaaggcga ggcaacacag ccttgcgctt ctaggcccat
     3361 ccgcagccac gtttttcgag ccggtgccga aggagcattt ctccaaggcg cttttcgaca
     3421 ctattgccca gtggaatgca gagtcggatt ggaagggtga cgagcggaac gtcgttcttg
     3481 ctcttgctcg catttggtac agcgcttcaa ctggtctcat tgctcctaag gacgttgctg
     3541 ccgcatgggt atcggagcgt ttgcctgccg agcatcggcc cctcatctgc aaggcacgcg
     3601 cggcgtacct gggtagcgag gacgacgacc tagcaatgcg cgtcgaagag acggccgcgt
     3661 tcgttcgata tgccaaagca acgattgaga gaatcttgcg ttgagcggca tgtgcgaaaa
     3721 gtgcatcgac ccgcgccgag ggcatctgat gcctaactcg gcgttcaagc ggacgggctg
     3781 cgcccgccgc tcaactatgc gttagatgca ctaagcacat aattgctcac agccaaacta
     3841 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     3901 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca
     3961 acatccgcat taaaatctag cgagggcttt actaagcttg ccccttccgc cgttgtcata
     4021 atcggttatg gcatcgcatt ttattttctt tctctggttc tgaaatccat ccctgtcggt
     4081 gttgcttatg cagtctggtc gggactcggc gtcgtcataa ttacagccat tgcctggttg
     4141 cttcatgggc aaaagcttga tgcgtggggc tttgtaggta tggggctcat aattgctgcc
     4201 tttttgctcg cccgatcccc atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg
     4261 tgttcggcat tctgaatctc accgaggact ccttcttcga tgagagccgg cggctagacc
     4321 ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg
     4381 tcggaccggc cgccagccat ccggacgcga ggcctgtatc gccggccgat gagatcagac
     4441 gtattgcgcc gctcttagac gccctgtccg atcagatgca ccgtgtttca atcgacagct
     4501 tccaaccgga aacccagcgc tatgcgctca agcgcggcgt gggctacctg aacgatatcc
     4561 aaggatttcc tgaccctgcg ctctatcccg atattgctga ggcggactgc aggctggtgg
     4621 ttatgcactc agcgcagcgg gatggcatcg ccacccgcac cggtcacctt cgacccgaag
     4681 acgcgctcga cgagattgtg cggttcttcg aggcgcgggt ttccgccttg cgacggagcg
     4741 gggtcgctgc cgaccggctc atcctcgatc cggggatggg atttttcttg agccccgcac
     4801 cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc
     4861 cgctattggt ctcggtgtcg cggaaatcct tcttgggcgc caccgttggc cttcctgtaa
     4921 aggatctggg tccagcgagc cttgcggcgg aacttcacgc gatcggcaat ggcgctgact
     4981 acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat caccttctcg gaaaccctcg
     5041 cgaaatttcg cagtcgcgac gccagagacc gagggttaga tcatgcctag cattcacctt
     5101 ccggccgccc gctagcggac cctggtcagg ttccgcgaag gtgggcgcag acatgctggg
     5161 ctcgtcagga tcaaactgca ctatgaggcg gcggttcata ccgcgccagg ggagcgaatg
     5221 gacagcgagg agcctccgaa cgttcgggtc gcctgctcgg gtgatatcga cgaggttgtg
     5281 cggctgatgc acgacgctgc ggcgtggatg tccgccaagg gaacgcccgc ctgggacgtc
     5341 gcgcggatcg accggacatt cgcggagacc ttcgtcctga gatccgagct cctagggatc
     5401 gcctcagaaa acggaaaata aagcacgcta agccgtaagt aagcgtgctc ctgtgaaagc
     5461 cacagctaaa actgcgtagt acacatagag gtattttttt ctagatttat atcgaatttc
     5521 gttatcgaat ttcgcttatt attgatcaac tcgttgcaga tcatgaaggc gaaagtatga
     5581 cgaataaccc taccgatgac agcagaccat ggtcggtctt tcttattttt ctgcggcttg
     5641 gattgacatc ttttggcggc cccattgcgc acttgggcta cttccgcgcc gaatttgtca
     5701 cacggcggcg ctggctctcc gaacggagct atgctgactt ggtcgcgctt tgtcagttct
     5761 tgccagggcc tgcaagcagc caggtcggca tagcggtagg actgtctcgg gctggataca
     5821 gcggggcgct ggctgcttgg gctggcttca cgctgccgtc tgccatagcc ttgatccttt
     5881 ttgcgctcgg catctccagc tatggcgatt acgtctcgca gggcgcgttg catggcttaa
     5941 aagtggtggc tgtggccgtg gtcgctcaag cagtatgggg catggcgcgt aacctatgca
     6001 cggatgggct gcgagtcacc atcatggcaa ttgctacctg cgtcgtttta cttgtgccgt
     6061 ccgcgtgggg acaggttggc gtgattgcta tcgcaggcat cgcaggccgg ttattgttca
     6121 agccagcgaa agttgttgag catgaccccc tacctatcac ggtcagtcac cgggccggcg
     6181 tgctttggct ctcgctgttc tttgtcttgc tgattggcct gccggtgttg gccgaactga
     6241 tgccaagtca aaccatggca atggtggatt ccttctatcg tgtcggatca ctggtgttcg
     6301 gcggtggtca cgttgtgctg ccattactgc aagccgaagt ggtgccctcc ggctgggtca
     6361 acaatgaatc ctttctcgcg gggtacgggg cagctcaagc ggtgcccggc cctttgttca
     6421 cgttcgccgc gtttcttggt gcctcgatga acaccgcccc gtcgggctgg atcggcggca
     6481 ttgtgtgtct gctggctatc ttcgcgccct cgttcttgct ggtcgtcgga tcaatgccat
     6541 tttgggagcg tttgcgccgc aatacaggca tccaagctgc gctggccggg atcaatgccg
     6601 ctgtagtcgg cttgctgctg gccgcgctgt atcagcctgt atggactagc gccatctttc
     6661 agccgcaaga cttcggcttg gcattagttg cccttgtcgc acttatgttc tggaagctcc
     6721 cgccgtggct ggttgtcctc ggtagcggtg cagctgggtg gctgttgagc gtcgctctgt
     6781 gaggcacaaa aaatgactga caaagacctc tacggcggtt tgatccgcct gcacatcctt
     6841 caccatgcag ccgaggaacc tgtctttggg ctggggatca tcgaagagct acgccgacac
     6901 ggctacgaga tgagcgctgg caccgtgtac ccgatgctgc acggcctgga aaaaaaaggc
     6961 tatctgacct cacgccacga acgcaccggg cgacgtgaac ggcgtgttta tgacatcact
     7021 gaacaaggca gaactgcact tgctgatgcg aaaacaaagg tcaaggagct gttcggagag
     7081 ttggtagaag gtggttgagt ttttgcttct atgtcaggtt gagctatacc ctaactggat
     7141 tgtcattttc agaagacgac tgcaccagtt gattgggcgt aatggctgtt gtgcagccag
     7201 ctcctgacag ttcaatatca gaagtgatct gcaccaatct cgactatgct caatactcgt
     7261 gtgggctctg ttgcaaaaat cgtgaagctt gagcatgctt ggcggagatt ggacggacgg
     7321 aacgatgacg gatttcaagt ggcgccattt ccagggtgat gtgatcctgt gggcggtgcg
     7381 ctggtattgt cgctatccga tcagctatcg cgaccttgag gaaatgctgg cggaacgcgg
     7441 catttcggtc gaccatacga cgatctatcg ctgggtccag tgctacgccc cggagatgga
     7501 gaagcggctg cgctggttct ggcggcgtgg ctttgatccg agctggcgcc tggatgaaac
     7561 ctacgtcaag gtgcggggca agtggaccta cctgtaccgg gcagtcgaca agcggggcga
     7621 cacgatcgat ttctacctgt cgccgacccg cagcgccaag gcagcgaagc ggttcctggg
     7681 caaggccctg cgaggcctga agcactggga aaagcctgcc acgctcaata ccgacaaagc
     7741 gccgagctat ggtgcagcga tcaccgaatt gaagcgcgaa ggaaagctgg accgggagac
     7801 ggcccaccgg caggtgaagt atctcaataa cgtgatcgag gccgatcacg gaaagctcaa
     7861 gatactgatc aagccggtgc gcggtttcaa atcgatcccc acggcctatg ccacgatcaa
     7921 gggattcgaa gtcatgcgag ccctgcgcaa aggacaggct cgcccctggt gcctgcagcc
     7981 cggcatcagg ggcgaggtgc gccttgtgga gagagctttt ggcattgggc cctcggcgct
     8041 gacggaggcc atgggcatgc tcaaccacca tttcgcagca gccgcctgat cggcgcagag
     8101 cgacagccta cctctgactg ccgccaatct ttgcaacaga gcccgccgtg ctagtctgct
     8161 cggtgatggt ggagtgaagc caacccgcaa tcgggttatg aatctgcatc gcgattcgca
     8221 atcagctgtc tcttgagcat gtcgaactcc tgcgatgtta tctcgccgtg ctcgtgctgc
     8281 tacaccaact tgtccaactc atccgccaca ccgctcccca ccacctgcga tggccaggtt
     8341 gtttgctggg cgcattggcg gagtatcgtc tcgacccggg acacccacac cggcacaacc
     8401 ttacgggagt gattcactgt caaagaatcg gcccggtgct ctgacgcaag tatcgggatg
     8461 gtcaccattt gtaagccgta gacctgagtg gtgatcaaga cttcgatacc accgaccgta
     8521 ccggtactaa tcgacgacgg tcgtgttcgt cgcctgccgc agggactctg cacacctccg
     8581 tttacgcatg tgcctggagg agttggaaat cgtcgtgttc gggaaacatt aaacacagga
     8641 tggcagcgat ctgagccagc acatgatcag ctagctcacc atccggatcg acggcccact
     8701 gcatcgtcgc gccagcgatg accgagtgca ggagcaactc agctgccgca ggagcacctg
     8761 ggggcagtcg cttgcggatc ccctccacca ccgcgcggtt ccgctggatc gcaagcgtgc
     8821 gtagctccgg cacctggagc tcgtaccagg agatgagata gttcaccgag aagtcgttgc
     8881 gagtgttcat gctccgaacg agcacctgca aaaattccca gagcccttgc ggccctgcgc
     8941 ctatcggtat cgcattcagg taatgccgca cctgctcgac gccgcgctcc atcatcctca
     9001 ccagcagcgt atcgcggttg gtgaagcgct ggattaacgc tgcgcgggag agccccacct
     9061 cctttgctac tccgctgagc gtgaactcta tgggaccgca acgcttcagc actacggtgg
     9121 cggcctcgag tacctcgtca tcggacttga gcttggggcg gggcatcagt gttcaccttc
     9181 tgtatgggtt ggggcggagg ctgtggctgc cgccgccatt gtagcaaatt gaagacggag
     9241 cgagagtaga gccacgagcc ccgcaaacac ggccgataca acgaggccag ggagcggacc
     9301 agcaaggtcg acaaacgcgc cggccgcaag cataaccatg ggcgaggctg acagcatcac
     9361 cgccgagacc gtgccgagta cccggccgag aagttctggc ggcgtgcggt tgtagatggc
     9421 agcgttgaga atgggagaga ctgagccggt cagcagtccc acgagcgcgc ccaacaacat
     9481 cagcaccggc acgcctggca actgtgaaag cagaagcgag cccaccgcag agccacaaaa
     9541 tgccaccgcc agccagttct gcgctgatat ccgggcgccg accgacgcat gaatggcaat
     9601 gccaaggaga ccaccagccc ccatcattga ggagaacagc ccgagctctg ctacttggcg
     9661 tcctgcatct acaaacagcg caggcatgat gacgctgccg ttggcgccaa cgatgcccac
     9721 gaagatcatc actataccaa agagagggcg cagcaggggt tcgctccaga gaaaagcgac
     9781 gccggcgcgc atggagagag tcgccgtcgt ggtcatcgtc cgagcggcac gcgcgggaag
     9841 cacccacgcg ccgagcagac ctgcaaggac ggagcagaac gccgtcagcc cgagcgttgg
     9901 cgcagcgcca agcaggccga ttgcggcccc cccaagggcc gggccaccta gaatcgcgac
     9961 gttcccgatc accgctttca gtgacgagac gcgctcaacg gagagcccgg cgacgtggcc
    10021 gagtttgggc agctcactgt cctgcgcggc cataccgggt gcgtcgaacg cggcaccgag
    10081 caccacgcaa gcgatcagcc cagtgttcga gagggcgcca acggcatcga gcagtgggat
    10141 gctcgccatg gccacgccgc ccaccacacc cgagatcaat gcgacgggcg cgcgcccgaa
    10201 ccgatcgacg aggccaccac caacccacgc gccgatgatg gtcgcgatga cgctgctagc
    10261 ggccgtggcg cccgcccagg ccgcgctctt tgtatgagac aggacgaacc atggaagcgc
    10321 gagggccgcc accgcgttgc cgatccggaa gagaaaggtc gccgcgaaca gcgtcgcgag
    10381 cgggctatat cgacgttcgc tcattccgct gcggcgagct gcgccttcgc cgcagcgagg
    10441 tactcttcgt tacccgagtc gagggcgaag agtgcgtagg tgaccgcccc gaacgcaagg
    10501 cgctccgcga tgtggtgggc gagccgcggc cacacccggc caccggccgc ttcatacgtg
    10561 aggaggagct tcgcgagccc ctcttcacca aagaccataa ggtgcgcggc catgtcgatg
    10621 gcagggtcat caacgcgggc ctcgctccag tcgatcatcc cgctgacgcg ctccgtgttg
    10681 tcgatgagca catggcccac gtagagatcg ccatgcacca ccacggagaa atctggccac
    10741 gacgaatcgt cgtcgagcca gcgctgccac cggtggaggc gcttgtcgtt caccacgaac
    10801 tcgcgtcgga cgcggtcaac gtcgtcggcc accttctgac gggcctgcgt cggtgtacgg
    10861 atgagcatcc ccgcatccac ggcggcggaa atggggacgg catgcagggc ggcgagcgcg
    10921 gtcgcgaagc tctccgcgaa gacctccgag tcctgcggca cgacccagtc gggcgtggac
    10981 gaaccaggct ggatgaccat cgcagtcgag tcttcgagca tgggataggc aacgagctcg
    11041 gcgttggcca cgcgccagtc cggcaccgcg aacggcaggc gattcttgag cattgccagc
    11101 acccgcgcct ctggttcgac cttcgcgctt acctcggctc ggcgcgggat gcgcagcacc
    11161 caccgacgtc catcgtcgac ggtggcgatc acgatcctat agtcgagccc aagctcattg
    11221 acagtcagcg ggccatggag cttgagccca tgtcgggctg caagtgcgta cagttgggag
    11281 gtatcggcgg tcgtgactac ggtcatgatt cactcctgag ggcttgacgg gtttagccac
    11341 ctaaatgtaa cagtcacgtc ggttatattc aatccggcac tgttgcaaag ttagcgatga
    11401 ggcagccttt tgtcttattc aaaggcctta catttcaaaa actctgctta ccaggcgcat
    11461 ttcgcccagg ggatcaccat aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat
    11521 cacctcaata cctttgatgg tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc
    11581 gccgattatc cgtttcagtt tgccatgatc gcattcaatc acgttgttcc ggtacttaat
    11641 ctgtcggtgt tcaacgtcag acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg
    11701 accataggcg ggcgctttat ccgtgttgat gaatcgcggg atctgccact tcttcacgtt
    11761 gttgaggatt ttacccagaa accggtatgc agctttgctg ttacgacggg aggaga
//
LOCUS       DAZPJR010000127        11807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPJR010000127 DAZPJR010000000
VERSION     DAZPJR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.128"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..487
                     /locus_tag="ACHVB7_003168"
     CDS             <1..487
                     /locus_tag="ACHVB7_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1915079.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            539..1474
                     /gene="ttcA"
                     /locus_tag="ACHVB7_003169"
     CDS             539..1474
                     /gene="ttcA"
                     /locus_tag="ACHVB7_003169"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR1915080.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHVB7_003170"
     CDS             complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHVB7_003170"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR1915081.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHVB7_003171"
     CDS             complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHVB7_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR1915082.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHVB7_003172"
     CDS             complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHVB7_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR1915083.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHVB7_003173"
     CDS             complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHVB7_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR1915084.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHVB7_003174"
     CDS             4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHVB7_003174"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR1915085.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHVB7_003175"
     CDS             complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHVB7_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR1915086.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6838..7746)
                     /locus_tag="ACHVB7_003176"
     CDS             complement(6838..7746)
                     /locus_tag="ACHVB7_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1915087.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7922..9232
                     /gene="abgA"
                     /locus_tag="ACHVB7_003177"
     CDS             7922..9232
                     /gene="abgA"
                     /locus_tag="ACHVB7_003177"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR1915088.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9232..10677
                     /gene="abgB"
                     /locus_tag="ACHVB7_003178"
     CDS             9232..10677
                     /gene="abgB"
                     /locus_tag="ACHVB7_003178"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR1915089.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10714..>11807
                     /locus_tag="ACHVB7_003179"
     CDS             10714..>11807
                     /locus_tag="ACHVB7_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR1915090.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3005 a         2999 c         2927 g         2876 t
ORIGIN      
        1 gaatgtcaga agaaacctga cgatacttga tatgttcggt cccccaaaaa caaatgccgg
       61 gatccggacg gtaacattac tgcagccggc tcttgaagca ctgaaggagc aatacaaact
      121 gaccgggcat catcgcaaaa gcgaaatcac tttttatcat cgggagtacg gcagaactga
      181 aaagcaaaaa ctgcattttg ttttcatgcc cagggtgtgt aacgaaaaac agaaacctta
      241 ttactcggta agcagtttgg gtgcaagatg gaatgcagca gtaaaacgtg ctggtattcg
      301 ccgccgtaat ccgtaccata cgcgacatac ttttgcctgc tggctgttga cggcaggagc
      361 gaacccggca tttatagcca gccagatggg gcatgaaact gcgcaaatgg tgtatgaaat
      421 ttacggtatg tggattgatg acatgaacga cgaacagata gccatgttga atgcgcggtt
      481 atcgtagttg caaagtttgc ccccaatttg ccccatttag taccagagaa ctgaaataat
      541 gcaagaaaat caacaaatta caaagaaaga acaatacaac ctgaacaaat tacaaaaacg
      601 tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg
      661 catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa
      721 tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca
      781 accgggcttc ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa
      841 gattgtcgaa gagaacactt acggtatcgt gaaagagaag attccagagg gcaaaaccac
      901 ttgctcactg tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg
      961 ggcgacgaag atcgcgctgg gtcaccatcg tgacgatatc ctgcagacgc tgttcttaaa
     1021 tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa
     1081 acatatcgtt attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga
     1141 tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg
     1201 tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac
     1261 gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga
     1321 tttcaaaggc atcacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg
     1381 cgaagagatc ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga
     1441 tgagttacgg ctgaatgtgg ttgaagtgaa ataaccagga tggcgcccga tgcgcaagcg
     1501 tatcgggcta ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatccc
     1561 gaataagatg tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac
     1621 ccggctcgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac
     1681 agcctgacgg accgcgacat agacatgcgc cggatgcacg gcgattttgc caatatctgc
     1741 gccatcaagc ccgatatctc ctgtcagtgc acctaataca tcacccgggc gcattttggc
     1801 ttttttccca ccatcgatac acaacgttgc catttctgct tccagcggca caatggaact
     1861 attagctggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa tgatattggc
     1921 ccgctgtgct tcttccggag cacagaaact gatcgccaga ccgctatttc ctgcacgagc
     1981 tgtacggccg atgcgatgta catgaacttc agggtcccac gccagctcaa agttcaccac
     2041 cagctcaagc gatttaatat ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg
     2101 ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct ccaaatcgcc
     2161 gtgtaatgac aatgcacttt gccctacttc attcagcgcg tcgcagacag cctggcaatc
     2221 ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta ataaccgttg
     2281 caacagagga attttgcctt tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa
     2341 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattac
     2401 ggcaatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     2461 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     2521 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     2581 acgacccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac gctgcatacc
     2641 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     2701 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     2761 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     2821 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     2881 ggcggcctgc accggcgtca tagttaaata acccaactca ttaaggttcg tgagttgggc
     2941 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     3001 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     3061 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     3121 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     3181 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     3241 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     3301 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     3361 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     3421 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     3481 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     3541 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     3601 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     3661 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     3721 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     3781 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     3841 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     3901 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     3961 gctggaatga tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg
     4021 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     4081 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
     4141 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgagacggct
     4201 gactcggggc cgcgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
     4261 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
     4321 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
     4381 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
     4441 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
     4501 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
     4561 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
     4621 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
     4681 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
     4741 ggatcgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
     4801 tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag
     4861 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
     4921 ttcagcgtaa agaagaggaa acagcattaa actgtcggct tgttttgcga aagctgacag
     4981 aagcagaacc ggtgattatt ttcgaaggta tcgaagcgtc ggcaacgctg ggtttaaaag
     5041 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
     5101 ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta
     5161 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
     5221 gggaaataaa tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
     5281 ccggtggtca taaacctctg aattttattc ataagctggc ggatggttcg actcgtcatg
     5341 tgcagaccta cgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtac
     5401 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgcc caccatgacg
     5461 cgatgaccgg attactgaat cggcgacagt tttatcacat taccgagcca ggccaaatgc
     5521 agcatctcgc catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac
     5581 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca
     5641 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
     5701 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
     5761 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
     5821 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
     5881 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
     5941 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
     6001 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgca gtgcaatatg
     6061 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
     6121 gcgatttatc atcccgtcct ttgccatgaa taatcagcac gttacgcaaa ccatcagcca
     6181 tggcttgttg aatgaaactg aacatcattt tgcggcactc ttccaccggc tggcgcaaaa
     6241 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt
     6301 gcaacccttc ccgccgaaac tccagcggct gacttagtgg gatgatgtcg agaaatccgg
     6361 tggtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
     6421 ttggatgcca gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
     6481 gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat
     6541 gataaccaga ccgggtcggt ccaacaacgt attacccaaa attccagtaa taagttccaa
     6601 atattgccga tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa
     6661 tgtaacttat attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac
     6721 cgttataaca cagtcaccgg cgcagaggag acaatgccgg atttaagacg cggatgcact
     6781 gctgtgtgta ctgtagagtc tggcggatgt cgacagactc tattttttta tgcagtttta
     6841 actttgcaga tagccgcatt ctcgccggaa ttgcgtgatt aatgacgcgg tcagtggtgt
     6901 ctggcgacta tcacgccgct gaatcaaata ataggccgct ttcggtaaaa tttcgctaac
     6961 cggcaacatc accagcccct gtccgtgcaa gggatcgcag cccatttctt caggcagtat
     7021 gctgagaaaa tcgctttttg ccaccagact gatacaggct gagaacgtct cgcagactac
     7081 accgacctgt ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt tgtagtaact
     7141 gccgtgtggc gtcggcattg tccagctgta atccagtaac tgtttgatcg aacgggcacc
     7201 aatggcgggg tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta atttctcaaa
     7261 agtaaattcg tggtcgtacg gtccctgata ataggtattg atggtgaaat ccaattctcc
     7321 ctgacgcaat tcattaatca tcgacaccag ttgcccttcc ataatgcgta cttttacctg
     7381 cggatgctgc tgatgaaaac gagatatgac agctggcatc agactgcggg aaatactggc
     7441 ccccatgccg atattaatct gccctgccag ttgcccttgt cgttggcgaa tatcctcttg
     7501 ggctgcgcgc agctcttcaa gaataagact ggcgtgctga taaaaacttt caccggcatc
     7561 agttaacgtc acgcctttac tacggcgaaa aaagagttgc gccgctaatc cttcttccag
     7621 ctcctgaata gatttactca gtgccggttg cgacatattc aacattcggc tcgctccgcg
     7681 aatgctgccc tgacgagcca cttcaacaaa agcccgaatt tgatgaattt ttacctgaaa
     7741 agccatgacg ccaccgataa ccgttattta tcagaccaaa gaaactggca tctactttaa
     7801 tgcagatgat tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa tcagttagtg
     7861 ataagtaaaa actatcgcta cgtgaaccgg gtcacacttt ttactgatga cgggaaaggt
     7921 tatggagtct ttgaatcaat ttgttaattc gcttgcccca aaattatcgc actggcgacg
     7981 tgattttcat cactatgcag agtctggctg ggtggaattc cgcactgcca cccttgttgc
     8041 ggaagaattg cagcagctcg gctattcact ggcgctgggc cgcgaagtag ttaatgaaag
     8101 tagccggatg ggactacctg atgaactcac tctacaacgc gaattcgagc gcgctcgtca
     8161 acagggtgcg ctagcacaat ggattgcggt ttttgaaggt ggtttcactg gtatcgtcgc
     8221 caccctggat accggtcgcc ccggtccggt gatggctttc cgtgtcgata tggacgcgct
     8281 ggatctcagt gaagagcagg atgtcagcca tcgcccctac cgcgacggtt ttgcgtcatg
     8341 taacgccgga atgatgcatg cctgtggtca tgatggacat accgccattg ggcttgggct
     8401 ggcgcatacc cttaaacaat tcgagtccgg actacatggc gtcatcaaac tgatttttca
     8461 gcctgcagag gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg tcgtagatga
     8521 tgttgattat tttactgccg tgcacattgg cactggcgta cctgcgggca ccgtggtgtg
     8581 cggcagtgat aattttatgg caaccaccaa atttgacgcg cacttcaccg gtaccgccgc
     8641 tcacgcaggc gcaaaaccag aagacggtca caatgccttg ttggcggcag cacaagccac
     8701 tcttgcactg catgcaatcg ccccgcacag cgaaggagct tccagagtaa acgtgggcgt
     8761 tatgcaggca ggaagcggtc gtaacgttgt tcctgcctcg gcgttgctga aagtggaaac
     8821 acgcggggcc agcgacgtca ttaatcaata tgtttttgac cgtgcacaac aagcgattca
     8881 gggcgcagca accatgtatg gtgtcggcgt tgaaactcgt ctgatgggtg cagttaccgc
     8941 cagttctcct tcgccgcaat gggtcgcatg gttgcaaagt caggcggctc aggtcgcggg
     9001 ggtcaatcag gccattgaac gtgttgaagc gcctgcgggt tccgaagatg ccacattaat
     9061 gatggcccgc gtgcagcaac atcaagggca agcctcctac gtggtgtttg gcacacagct
     9121 ggcggcaggt catcacaacg aaaaattcga ttttgacgag caggttctcg ctattgccgt
     9181 cgaaacgctg gcgcgcaccg cgctcaattt tccctggacg cgaggtatct gatgcaggaa
     9241 atctatcgtt ttatcgacga tgcgattgaa gccgatcgcc aacgttatac cgatattgcc
     9301 gatcaaatct gggatcatcc agaaacacgt tttgaagagt tctggtcagc ggagcatctg
     9361 gcttcggctc tggaatctgc aggcttcacc gttacccgca acgtaggcaa tatcccaaat
     9421 gcctttattg cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg agaatatgac
     9481 gccctggcag gtttaagtca gcaagctggt tgcgcgcaac ctacatccgc gacgcccggt
     9541 gaaaatggtc acggttgcgg acacaatttg cggggaaccg ccgcctttgc cgctgcaata
     9601 gccgtcaaga aatggctgga acaatatggg caaggcggca cagtgcgctt ttatggttgt
     9661 cctggcgaag aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt atttgatgat
     9721 gtggatgcgg cactcacctg gcacccggaa gcctttgccg gtatgttcaa tacccgcacg
     9781 ctggcaaaca ttcaggcatc atggcgcttt aaagggatcg cagcacatgc cgcgaattcc
     9841 cctcatttgg gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg caccaacttc
     9901 ctcaacgaac atattattga aaaagcgcgc gtacactatg ccatcacaaa tagcggcggg
     9961 atctcgccca acgtggtcca ggcgcaggca gaagtgcttt atcttatccg cgcccccgaa
    10021 atgaccgacg tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg tgcggcattg
    10081 atgaccgaaa ccacggttga atgccgcttc gacaaagcct gttccagtta tctcccgaat
    10141 cgcaccttag aaaatgccat gtaccatgcc ctatcccatt ttggtacccc ggaatggaac
    10201 tccgaagaac tggcttttgc gaaacaaatt caggctacgc tcacccccaa cgatcggcaa
    10261 aacagtctga ataatatcgc cgcaaccggt ggcgaaaacg gcaaggcttt tgcactacgt
    10321 catcgtgaaa cggtactggc gaatgaggtc gctccatatg ccgccaccga taacgtgctt
    10381 gcggcatcga ctgatgtcgg cgacgttagt tggaaactgc ctgttgccca gtgtttcagc
    10441 ccctgttttg ccgtcggtac accgctacat acgtggcaac tggttagcca ggggcgaaca
    10501 tctattgctc ataaaggaat gctgctggcg gcgaaaacta tggcagcaac cacagtcaat
    10561 ctcttcattg attcagggct attgcaagaa tgccaacaag agcatcagca agtaacggac
    10621 acgcaaccgt atcactgccc tatcccgaaa aacgtgacac cgtcaccttt aaaataacaa
    10681 caacaacgca aacacaacaa ccgaggaatg cccatgagta tgtcatccat accgtcgtcc
    10741 tcccaatccg ggaagctcta tggctgggtc gaaagaattg gtaacaaggt tctccatcct
    10801 tttttgctct ttatctattt gattatcgta ctcatggtga cgacggcaat tttgtcggcc
    10861 tttggcgtca gtgcgaaaaa cccgaccgat ggaacgccgg tggtggtgaa aaacctgctc
    10921 agtgtggaag gattacactg gtctttaccc aatgttatta aaaactttag cggttttgct
    10981 ccacttggtg cgatcctggc gctggtttta ggtgccggtc tggcggagcg cgtcggctta
    11041 ctgccagcgc taatggttaa aatggcatcg catgttaatg cccgctacgc cagttatatg
    11101 gtgctgttta ttgctttttt cagccacatt tcttccgatg cgacgttagt gatcatgcta
    11161 ccgatgggtg cgctgatttt tctggcggtg ggcaggcatc cagttgcagg tttactggct
    11221 gccattgcag gcgtaggttg cggctttacg gctaatttac tgattgtcac aaccgacgtg
    11281 ttgctgtcgg ggatcagcac ggaagcggcg gctgcgttca atccgcaaat gcacgtcagt
    11341 gtaattgata actggtattt tatggccagc tccgtagtcg tactgacgat tgttggcggc
    11401 ctgataaccg acaaaatcat cgagccacgg ttaggtcaat ggcagggaaa cagcgatgag
    11461 aaactgcaga cattgaccga aagtcagcgt tttggtttac gcatagcagg tgtcgtatcg
    11521 ctacttttta ttgctgcgat tgcgctgatg gtgatcccgg aaaacgggat attgcgcgat
    11581 ccgattaatc acaccgtgat gccatcaccc tttattaaag gtatcgtgcc actgatcatt
    11641 ctttttttct ttgttgtctc gctggcttat ggcatcgcta cccgcacaat tcgacgtcag
    11701 gcggatttac cgcagttaat gattgaaccg atgaaagaga tggcaggatt tatcgtgatg
    11761 gtttttcccc tcgcccagtt tgtcgccatg tttaactgga gcaacat
//
LOCUS       DAZPJR010000128        11801 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPJR010000128 DAZPJR010000000
VERSION     DAZPJR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11801
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.129"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..296
                     /locus_tag="ACHVB7_003180"
     CDS             <1..296
                     /locus_tag="ACHVB7_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR1915091.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
     gene            791..1690
                     /gene="lysO"
                     /locus_tag="ACHVB7_003181"
     CDS             791..1690
                     /gene="lysO"
                     /locus_tag="ACHVB7_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR1915092.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1834..3486
                     /gene="hcp"
                     /locus_tag="ACHVB7_003182"
                     /gene_synonym="priS"
     CDS             1834..3486
                     /gene="hcp"
                     /locus_tag="ACHVB7_003182"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR1915093.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3498..4466
                     /gene="hcr"
                     /locus_tag="ACHVB7_003183"
     CDS             3498..4466
                     /gene="hcr"
                     /locus_tag="ACHVB7_003183"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR1915094.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4599..6317
                     /gene="poxB"
                     /locus_tag="ACHVB7_003184"
     CDS             4599..6317
                     /gene="poxB"
                     /locus_tag="ACHVB7_003184"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR1915095.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6354..7355
                     /gene="ltaE"
                     /locus_tag="ACHVB7_003185"
     CDS             6354..7355
                     /gene="ltaE"
                     /locus_tag="ACHVB7_003185"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR1915096.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7366..8796
                     /locus_tag="ACHVB7_003186"
     CDS             7366..8796
                     /locus_tag="ACHVB7_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR1915097.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8895..9908
                     /locus_tag="ACHVB7_003187"
     CDS             8895..9908
                     /locus_tag="ACHVB7_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1915098.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="ACHVB7_003188"
     CDS             complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="ACHVB7_003188"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR1915099.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10732..11055)
                     /locus_tag="ACHVB7_003189"
     CDS             complement(10732..11055)
                     /locus_tag="ACHVB7_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR1915100.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(11266..>11801)
                     /locus_tag="ACHVB7_003190"
     CDS             complement(11266..>11801)
                     /locus_tag="ACHVB7_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1915101.1"
                     /translation="SFGSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFHDCSMHG
                     VKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFLEIIFSL
                     KNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRYIPQSQD
                     GEDFRSHLQYKLPLSAH"
BASE COUNT         2708 a         3109 c         3094 g         2890 t
ORIGIN      
        1 cgctggtagt tgaactggta ttgagtgcag gtttcctgtt ggtgatccac ggcgcaaccg
       61 acaaattcgc gccggcaggt tttgcgccga tcgctattgg tctggcctta accctgattc
      121 acttaattag tattccggtg actaacactt ctgttaaccc ggcgcgcagc accgcggttg
      181 ctatcttcca gggcggctgg gcattagaac aactgtggtt cttctgggtg gtgccaattg
      241 tcggcggcat tatcggtggt ctgatttacc ggaccctgct ggaaaagcgt gattaattaa
      301 cgcagaaggc ctggcgtaat gtcaggcctt tttttaagat gttgttatat gttgattcat
      361 catcttgttg cgctcctttc atgagctaag ccacatattg ccactggcgc aaggagcgcg
      421 cagggggcgg ccaatcgccg ccgccccctg cccccccggg ctctggcgaa caaaatcgcc
      481 gctgcgcggt gccctcgact tatcccttac ggctaccggg tcgggcgcga ggtaacatcc
      541 ctgtaaaacg cgccctcagc ccacatccat gtgggctgcc ccggccttca gggaacgcct
      601 cggcgatttt gacgccacca acatcggtgc agtttgtaat taaagcgcaa gaatatcttt
      661 aaatgaaaac gaatactcta ctgctcctta tgcataacat ttccttttaa ttgcaacttt
      721 actcgtcctt ccgctttcag tagtgtgtca tcgcgcattt cgacttcatc tttgcaagga
      781 ctgggttttc atgttttctg ggctgttaat cattctggtt cccctgattg taggttacct
      841 cattccgctt cgccaacaag ctgcgttaaa agttattaat cagctattaa gctggatggt
      901 ttaccttatt ctctttttta tgggtatcag tctggcgttt ctcgataacc tcgccagtaa
      961 cctgttggcg attctgcatt attctgccgt cagtattacc gttattttac tgtgtaatat
     1021 tgccgccctg atgtggctgg agcgaggcct gccgtggcgc aaccaccatc agcaagaaaa
     1081 actcccgtcg cgtattgcga tggcgctgga gtcgctaaaa ctgtgcggcg tagtagtgat
     1141 tggttttgcc attggtctga gtggactggc tttcttacaa cacgcgaccg aagccagtga
     1201 atacacgtta attttgctac ttttcctcgt tggtattcag ttgcgcaata atggcatgac
     1261 cttaaagcag attgtcctta atcgccgggg aatgattgtc gccgtggtgg tggttgtcag
     1321 ttcattaatt ggtggtttaa ttaacgcctt tattcttgat ctccccatca ataccgcgct
     1381 ggcaatggcc tccggtttcg gctggtattc tctttccggt attttattga ccgaatcttt
     1441 tggtccggta atcgggagcg cggcgttttt taatgatctg gcccgtgaac tgattgctat
     1501 tatgttgatc cctgggctga ttcgccgcag ccgctctact gcactgggct tatgcggtgc
     1561 cacatcaatg gatttcaccc tgcccgttct tcaacgtact ggcgggctgg atatggtccc
     1621 ggcggcaatt gttcacggtt ttattcttag cctgttagtg ccgatcctca tcgccttttt
     1681 ctctgcgtaa tacctctctg gcggtagatc cctgccgcca aaattgcgct aaatcaatct
     1741 cccttaaagt tgcatgaaaa atccctttta tccccgcgtt aagcgtctta accttaaaca
     1801 tgtatattaa atataacttt aaaaggtgtg atcatgtttt gtgtgcaatg tgaacaaact
     1861 atccgtactc cggcaggaaa cggctgctca tacgcgcagg ggatgtgtgg taaaacggcg
     1921 gaaacttctg accttcagga tttactcatc gcggcgctgc aagggctttc tgcctgggcg
     1981 gtaaaagcgt gtgaatacgg catcatcaac cacgatgttg acagcttcgc gccacgtgcc
     2041 tttttctcaa ccctgaccaa cgttaacttc gattctccgc gtattgtcgg ctacgctcgt
     2101 gaagcgattg ccttgcgcga ggcgctgaaa gcacaatgcc tggctgtaga tgccaacgcg
     2161 cgcgtcgata acccgatggc tgacctgcaa ctggtgagcg atgatctcgg cgagctgcaa
     2221 cgtcaggcag cagaatttac ccctaacaaa gataaagcgg cgattggcga aaacattctc
     2281 ggcctgcgtc tgctgtgcct gtatggcctg aaaggtgcgg cggcctatat ggaacacgcg
     2341 catgttctcg gtcaatacga caacgatatt tatgcccagt accataaaat catggcgtgg
     2401 ctggggacct ggcctgccga tatgaacgcg cttcttgagt gttcaatgga aatcggccag
     2461 atgaacttca aagtgatgag cattctggat gcaggcgaaa ccggtaaata cggtcacccg
     2521 acgccaaccc aggtcaacgt caaagcgacg gcgggtaaat gcattctgat ttccggtcac
     2581 gatctcaaag atctctacaa cctgctggaa cagaccgaag gcacgggcgt taatgtctac
     2641 acccacggcg aaatgctgcc tgcgcatggc tacccggagc tgcgtaaatt caagcatctg
     2701 gtcggtaact acggcagcgg ctggcagaat cagcaagtgg agttcgctcg tttccctggc
     2761 cccatcgtga tgacctcgaa ctgcatcatc gacccaaccg taggcgctta tgacgatcgt
     2821 atctggaccc gcagcattgt tggctggcct ggcgtgcgtc atctggacgg tgatgatttc
     2881 tctgcggtta tcacccaggc gcaacagatg gcaggcttcc cgtacagcga aattccgcac
     2941 cttatcaccg tgggttttgg tcgccagacg ctgcttggcg ctgctgatac gctgattgat
     3001 ctggtgagcc gtgaaaaact gcgtcatatc ttcctgcttg gtggctgtga cggcgcacgc
     3061 ggcgagcgcc actacttcac cgatttcgcc accagcgtgc cggatgactg cctgatcctg
     3121 accctcgcct gtggtaaata tcgctttaac aaacttgagt ttggcgatat cgaaggtctg
     3181 ccgcgtctgg tagatgcagg tcaatgtaac gatgcttact cagcgattat tctggctgtc
     3241 actctggcag agaaactggg ctgtggtgtg aacgatctgc cgctgtcgct ggtgctctcc
     3301 tggtttgaac agaaagcgat cgtcattctg ctgacgctgc tttctctggg cgtgaaaaat
     3361 atcgtcaccg gcccgactgc acctggtttc ctgacaccag acctgctggc ggtgctgaac
     3421 gagaaattcg gcctgcgttc tatcaccact gttgaagaag acatgaagca actgttgagc
     3481 gcgtaaggag tttgtcgatg acgatgccaa cgaatcaatg cccgtggcgg atgcaggttc
     3541 atcacattac gcaagaaacg ccggatgtgt ggacgatttc cctgatttgc cacgattact
     3601 acccatatcg cgccgggcaa tatgcactgg tcagcgtgcg aaactcagcg gaaacgctgc
     3661 gtgcttacac catttcctcc acgccaggcg tgagtgaata tatcaccctg accgtgcggc
     3721 ggattgatga cggtgtcggc tcccagtggc tgacgcgcga tgtaaaacgc ggtgattatc
     3781 tctggctttc ggacgcgatg ggggaattta cctgcgacga taaagcagaa gataaattcc
     3841 tgttgctggc ggcaggctgc ggcgtcacgc cgattatgtc gatgcgtcgc tggcttgcga
     3901 agaaccgtcc acaggccgat gtgcgggtga tctacaacgt gcgtacgccg caggatgtta
     3961 ttttcgccga tgagtggcgt aactatccgg taacgctggt ggcagaaaat aacgttaccg
     4021 aaggctttat cgctggtcgt ctcactcgcg aactgctggc aggtgtacct gacttagctt
     4081 cacgtaccgt gatgacctgc ggcccggctc cgtatatgga ttgggtagag caggaagtga
     4141 aagcgctcgg cgtgacgcgt ttctttaaag agaaattctt cactccagta gcagaagcgg
     4201 cgaccagcgg tctgaaattc accaaactgc aaccggcacg agaattttac gccccggttg
     4261 gcaccacgct actggaggcg ctggaaagca ataacgttcc ggttgtcgct gcctgccgtg
     4321 cgggtgtttg cggctgctgt aagacaaaag tggtttccgg tgaatatacg gtgagcagta
     4381 caatgacgct gaccgacgcc gaaatcgctg aaggttacgt actggcctgc tcctgccatc
     4441 cgcaggggga tttggttctc gcataatcgc cttatgcccg atgatattcc tttcatcggg
     4501 ctatttaacc gttagtgcct cctttctctc ccatcccttc cccctccgtc agatgaacta
     4561 aacttgttac cgttatcaca ttcaggagat ggagaaccat gaaacaaacg gttgcagctt
     4621 atatcgccaa aacactcgaa tcggcagggg tgaaacgcat ctggggagtc acaggcgact
     4681 ctctgaacgg tcttagtgac agtcttaatc gcatgggcac catcgagtgg atgtccaccc
     4741 gccacgaaga agtggcggcc tttgccgctg gcgctgaagc acaacttagc ggagaactgg
     4801 cggtctgcgc cggatcgtgc ggccccggca acctgcactt aatcaacggc ctgttcgatt
     4861 gccaccgcaa tcacgttccg gtactggcga ttgccgctca tattccctcc agcgaaattg
     4921 gcagcggcta tttccaggaa acccacccac aagagctatt ccgcgaatgt agtcactatt
     4981 gcgagctggt ttccagcccg gagcagatcc cacaagtact ggcgattgcc atgcgcaaag
     5041 cggtgcttaa ccgtggcgtt tcggttgtcg tgttaccagg cgacgtggcg ttaaaacctg
     5101 cgccagaagg ggcaaccacg cactggtatc atgcgccaca accagtcgtg acgccggaag
     5161 aagaagagtt acgcaaactg gcgcaactgc tgcgttattc cagcaatatc gccctgatgt
     5221 gtggcagcgg ctgcgcgggg gcgcataaag agttagttga gtttgccggg aaaattaaag
     5281 cgcctattgt tcatgccctg cgcggtaaag aacatgtcga atacgataat ccgtatgatg
     5341 ttggaatgac cgggttaatc ggcttctcgt caggtttcca taccatgatg aacgccgaca
     5401 cgttagtgct actcggcacg caatttccct accgcgcctt ctacccgacc gatgccaaaa
     5461 tcattcagat tgatatcaac ccagccagca tcggcgctca cagcaaggtg gatatggcac
     5521 tggtcggcga tatcaagtcg actctgcgtg cattgcttcc attggtggaa gaaaaagccg
     5581 atcgcaagtt tctggataaa gcgctggaag attaccgcga cgcccgcaaa gggctggacg
     5641 atttagctaa accgagcgag aaagccattc acccgcaata tctggcgcag caaattagtc
     5701 attttgccgc cgatgacgct attttcacct gtgacgttgg tacgccaacg gtgtgggcgg
     5761 cacgttatct aaaaatgaac ggcaagcgtc gcctgttagg ttcgtttaac cacggttcga
     5821 tggctaacgc catgccgcag gcgctgggtg cgcaggcgac agagccagaa cgtcaggtgg
     5881 tcgccatgtg cggcgatggc ggttttagca tgttgatggg cgatttcctc tcagtagtgc
     5941 agatgaaact gccagtgaaa attgtcgtct ttaacaacag cgtgctgggc tttgtggcga
     6001 tggagatgaa agctggtggc tatttgactg acggcaccga actacacgac acaaactttg
     6061 cccgcattgc cgaagcgtgc ggcattacgg gtatccgtgt agaaaaagcg tctgaagttg
     6121 atgaagccct gcaacgcgcc ttctccatcg acggtccggt gctggtggat gtggtggtcg
     6181 ccaaagaaga gttagccatt ccaccgcaga tcaaactcga acaggccaaa ggatttagct
     6241 tgtatatgtt gcgcgcaatc atcagcggac gcggcgatga agtgatcgaa ctggcgaaaa
     6301 cgaactggct aaggtaaaaa gggtggcatt tcccgtcata ataaggacat gccatgattg
     6361 atttacgcag tgataccgtt acccgaccga gccgcgccat gctcgaagca atgatggccg
     6421 ccccggttgg ggacgacgtt tacggagacg accctaccgt taatgctctg caggactacg
     6481 ccgcagagct ttccggtaaa gaagccgcca tttttctgcc gaccggcacc caggctaacc
     6541 tggtcgctct gctcagtcac tgcgaacgcg gcgaagagta tattgtcggt caggccgcgc
     6601 ataattatct gtttgaagcc ggtggcgcag cggtgctggg cagtattcaa ccgcaaccca
     6661 tcgacgcggc tgccgacggc acgctaccgc tggataaagt ggcgatgaaa atcaaacccg
     6721 acgatatcca tttcgcccgc accaaattac tcagtctaga aaacacccac aacggcaaag
     6781 tgctgccgcg tgaatacctg aaagaagcat gggaatttac ccgcaagcgc aatctggcgc
     6841 tgcatgttga cggtgcgcgc atctttaatg ccgtggtggc ttacggctgc gaactgaaag
     6901 agatcacgca atattgtgat tcgttcacca tttgcctgtc gaaaggtctt gggacgccag
     6961 tcggttcatt actcgtcggt aatcgtgatt acattaaacg tgccatccgc tggcggaaaa
     7021 tggccggtgg cgggatgcgt cagtccggga ttctggctgc cgccgggatg tatgccctga
     7081 aaaataacgt cgcacggttg caggaagatc acgacaacgc cgcctggatg gcggagcaac
     7141 tgcgtgaagc aggcgcggat gtgatgcgtc aggacactaa tatgctgttt gttcgcgtcg
     7201 gggaagaaaa tgctgccgcg ttaggcgaat acatgaaagc gagaaacgtg ctgattaacg
     7261 cctcgccgat tgtccgcctg gtgacgcatc ttgacgtctc gcgcgaacaa ctggcggaag
     7321 tcgccgccca ctggcgcgca ttcctggcgc gttaaggaga gagacgtgcc gcaacgcatt
     7381 ttagttctcg gtgccagtgg ctacattggt cagcatctgg tgcgcacact cagccagcaa
     7441 gggcatcaga tcctggcggc ggcacgtcat gtcgacaggc ttgcaaagct gcaactggca
     7501 aacgtcagtt gccataaagt cgatctcagc tggccggata accttccggc cctgttgcag
     7561 gatatcgata ccgtctattt tctggtgcac agcatgggcg aaggcggcga ttttatcgct
     7621 caggagcgcc aggtggctct caacgtccgc gatgcgctac gtgaagtacc agttaagcaa
     7681 ttaatctttc tcagttcgtt gcaggccccg ccacatgagc agtcggatca tctgcgtgct
     7741 cgtcaggcta cggcggacat tcttcgtgaa gcgaatgtac ctgtgaccga acttcgggcc
     7801 ggaattatcg ttggcgcagg ttcagcggcg ttcgaagtca tgcgcgatat ggtctacaac
     7861 ctgccagtgt taacgccgcc acgctgggta cgttcacgca ccacgcccat cgcgctggaa
     7921 aacttgctgc actatctggt ggcgttgtta gatcatccgg ccagcgaaca ccgcatcttc
     7981 gaagccgccg gaccagaggt gctcagttat cagcaacagt ttgaacattt tatggcggtg
     8041 agcggtaagc gccgctggtt gatccccatc cccctcccca cccgctggat ttcggtgtgg
     8101 tttctcaatg tgattacttc cgtaccgccc accaccgcca gggcgttgat tcaggggctg
     8161 aaacacgatc tgctggcgga tgataccgcg ctacgtgcac tcatcccaca acggctgatc
     8221 gctttcgatg acgcggtacg tagcacgttg aaagaggagg aaaaactggt caactccagc
     8281 gactggggct acgacgctca ggcctttgcc cgctggcgac cggagtacgg ttattttgcc
     8341 aaacaggcgg ggtttaccgt taaaacgtcc gccagccttg ctgctttatg gcaggtagtg
     8401 aaccaaatcg gcggtaaaga gcgttatttc tttggcaata ttttgtggca gacacgggcg
     8461 ttgatggacc gcgcgatcgg tcataagctg gcgaaaggcc gcccggagcg cgaatattta
     8521 cagactggcg atgcggtgga tagctggaaa gtgattgtcg ttgaaccgga aaaacaactt
     8581 acgttgttat ttggcatgaa agcgtcgggg ctgggacgac tgtgttttag cctggaagat
     8641 aaaggcgact atcgtactat cgatgtccgc gctttctggc atccgcacgg tatgccgggg
     8701 ctgttttact ggttattgat gatccccgcg catctgttta tttttcgcgg aatggcaaaa
     8761 caaatcgcca gactggcaga acaaagcaca gattaatcga caaaacctgc gttattcttt
     8821 catgtttccc attgcatcat gggggaaatc acggaagaat gcgcacgtca ttttctcaac
     8881 aacagtggat tgatatgaag gtactggtta ccggcgccac cagcggctta ggtcgaaacg
     8941 cggtagagtt tttatgccag aaaggcatca gcgtgcgagc gaccggtcgc aacgaggcaa
     9001 tgggcaaatt gctggaaaaa atgggcgcag agtttgttcc ggcggatctg accgagctgg
     9061 tttcatcaca agctaaagtg atgctcgcgg gcattgatac gctgtggcac tgctccagct
     9121 ttacctcacc ctgggggaca caacaggctt tcgatctggc taacgttcgc gccactcgcc
     9181 gcctgggtga atgggctgtc gcctggggtg tgcgtaactt tattcatatc tcttccccct
     9241 ccctgtactt cgattatcac caccatcgcg atattaaaga agattttcgc cctcaccgct
     9301 tcgccaacga gtttgcccgc agcaaagcag ccagcgaaga agtgatcaat atgctttcgc
     9361 aggcgaatcc acaaacgcgc tttactattc tgcgcccaca aagtctgttc ggaccgcacg
     9421 ataaagtctt tattccccgt ctggcgcata tgatgcacca ctacggcagt attctgttac
     9481 cgcatggcgg cagtgcgctg gtcgatatga cctactatga aaataccgtg cacgcaatgt
     9541 ggctggcaag ccaggaagcc tgcgataagc taccttccgg gcgtgtgtac aatatcacca
     9601 acggcgagca tcgcacactg cgcagcatcg tgcagaagct gatcgacgag ttgaatattg
     9661 actgtcgtat tcgttccgtc ccctacccga tgctggatat gatcgcccgc agcatggagc
     9721 gtttaggccg caagtcagca aaagagccgc cgctgaccca ctacggcgtc tccaagttga
     9781 attttgactt tacgctggat attacgcgtg cgcaggaaga gttaggttat cagccgatca
     9841 tcaccctaga tgaaggtatc gagaaaactg ccgcctggct acgcgaccac ggaaaactgc
     9901 cgcgctaatc ctgcccgtat ttctccagca atgcttcggc aatcgcctga gtttctgcat
     9961 ccgcttcgcc gttatataac gtcgggcgga aatgcatctg gaatgccata atcacgcgcc
    10021 gctgctcgcg cggtgtcata tcaggtttaa cgtcataacc gtagcgcgcc aaaagctcca
    10081 gcaatgacgc ggtatctacc ggtgtgtgcg gcgcgcgccc ggcaaggtaa aagttaaccc
    10141 gctgcgcatc cggccaggca ccaatcccct gctgcgccag ttgctgccag ggaaataatg
    10201 gccccggatc gtctttgcgc tgcggtgcga tatccgcatg tgccactacg ttttccggct
    10261 tgatgtgata acgggcaata atatctttcg ccagtggaat aagcgcctga atctgtgtcg
    10321 gttcaaacgg ggcaaaatat ttcactccgg ccgatttttg ccagccacgg ttttccagct
    10381 caatgccaat agaggtgtcg ttaaggcgcg ttgccccgcg ccaggcgcta atccccgcat
    10441 gccaggccag ttcttgttcc ggcaccagtt gccagatgcg cggtttaccg ttgtatcgcg
    10501 gtggtaccgc agggaccaga taatgcgagc tgacctgctt atcggtcagt gtcgccagcg
    10561 agctatcaaa atcatctgcg gtgtagtgga tcaccagcac tttaatgcgc ggatacgccg
    10621 cctgcgcctg gcgtcgggta tcaagctgat atccctcttt ctcgacaatg cctttttcgc
    10681 ctgcacaccc tgccaataac agagcggcag cgaccagcca gaagactctt ctcatcgacg
    10741 cgttttcacc gccgtaccgc tgacgctaac catcagcata ctgccgtttt gcccgaccgt
    10801 ttcgtagtcg atatcaatac cgacgacggc atcggccccc agcgcccgcg cctgggagcc
    10861 taattcctca aaggcgatct cccgtgcttt acgcagttct ttttcatacg caccggaacg
    10921 tccgccaacg atatcgcgga taccggcaaa gaaatcacgg aaaatattgg cacctaaaat
    10981 cgcttcgccg gtcaccacac cgcaatattc aacgatggtc tggccttcca gagttggggt
    11041 tgtagaaaat tgcatgatcg atctccttgt ttgttaagcc ttcgatctct aaagcattat
    11101 cgaactgata tgccatgttg ggaagaaggt tttgatcagt ttaggaaatc atctcattgt
    11161 gtcaaaaaca cccacttaag tctttacgat tatccgttgc ataacccgat attggtttca
    11221 ttatagggta ccgcttacct gtcttattat gttgcaccaa caacattaat gagcggaaag
    11281 aggcaactta tattgtaaat gactcctaaa gtcttcccca tcttgtgact gaggtatata
    11341 cctctggatt gctttttggt cagacgccgc aaaacaagaa agtaataaga tctcaatgct
    11401 tttgtcatta atataagcga agatcattct tgcaaattcg taaataaacg cccgttcctg
    11461 agcgcttaat agtttaatgc aatatttatt cttaagagag aaaataattt caaggaaagc
    11521 atccgcagca accttcacgt tattcccggc gctcatatta tatatatccc ttgcacagat
    11581 agagtacaga gatagtaaac agccatacaa ataatcattt attaactctt ttgtccattt
    11641 tactggcaac catggtttta tcttgacacc atgcatacta cagtcatgaa aaagacattt
    11701 gctgaaatta actcttatca tatcaacatt aaaaaaatgg caattaataa attttaaaga
    11761 atgagtattt acgttgttaa ttttaacaga tccaaacgaa a
//
LOCUS       DAZPJR010000129        11779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPJR010000129 DAZPJR010000000
VERSION     DAZPJR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.130"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /locus_tag="ACHVB7_003191"
     CDS             complement(<1..611)
                     /locus_tag="ACHVB7_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR1915102.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHVB7_003192"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHVB7_003192"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1915103.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHVB7_003193"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHVB7_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR1915104.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHVB7_003194"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHVB7_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR1915105.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /locus_tag="ACHVB7_003195"
     CDS             complement(5085..6716)
                     /locus_tag="ACHVB7_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR1915106.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHVB7_003196"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHVB7_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR1915107.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="ACHVB7_003197"
     CDS             7139..7411
                     /locus_tag="ACHVB7_003197"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1915108.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHVB7_003198"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHVB7_003198"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1915109.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHVB7_003199"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHVB7_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR1915110.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHVB7_003200"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHVB7_003200"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1915111.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHVB7_003201"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHVB7_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR1915112.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11779)
                     /locus_tag="ACHVB7_003202"
     CDS             complement(11524..>11779)
                     /locus_tag="ACHVB7_003202"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1915113.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2856 c         2892 g         2987 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcagga gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggtgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gtgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtc
//
LOCUS       DAZPJR010000130        11709 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPJR010000130 DAZPJR010000000
VERSION     DAZPJR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.131"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(270..1013)
                     /locus_tag="ACHVB7_003203"
     CDS             complement(270..1013)
                     /locus_tag="ACHVB7_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1915114.1"
                     /translation="MPSIPSFVLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRA
                     RLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALA
                     ENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAK
                     LPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
                     TIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="ACHVB7_003204"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="ACHVB7_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR1915115.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="ACHVB7_003205"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="ACHVB7_003205"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR1915116.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /gene="dpaA"
                     /locus_tag="ACHVB7_003206"
     CDS             1926..2666
                     /gene="dpaA"
                     /locus_tag="ACHVB7_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR1915117.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="ACHVB7_003207"
     CDS             complement(2637..3404)
                     /locus_tag="ACHVB7_003207"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR1915118.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="ACHVB7_003208"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="ACHVB7_003208"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR1915119.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="ACHVB7_003209"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="ACHVB7_003209"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR1915120.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="ACHVB7_003210"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="ACHVB7_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR1915121.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="ACHVB7_003211"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="ACHVB7_003211"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR1915122.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVAWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..9490)
                     /locus_tag="ACHVB7_003212"
     CDS             complement(8354..9490)
                     /locus_tag="ACHVB7_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR1915123.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(9959..10336)
                     /locus_tag="ACHVB7_003213"
     CDS             complement(9959..10336)
                     /locus_tag="ACHVB7_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915124.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            complement(10336..10773)
                     /locus_tag="ACHVB7_003214"
                     /pseudo
     CDS             complement(10336..10773)
                     /locus_tag="ACHVB7_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10784..11709)
                     /locus_tag="ACHVB7_003215"
                     /pseudo
     CDS             complement(10784..11709)
                     /locus_tag="ACHVB7_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
BASE COUNT         2905 a         2893 c         2759 g         3152 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtggc atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agcgatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     8521 ttttgcagtc gtcttcattc attaccacgt ccagacgcca gtgcagctta ttctccacgt
     8581 gccagtggtt tcggattgct gtggcgaact tctctgcggt taaatcagca gaactgatat
     8641 aatatctgac cgtcatttct ggctctttct tttgttctgc tattatggac cgaaaggaga
     8701 ctgccacgca taatttcttc agccctttcc attcaaacgt gaaatcaata agttcatcag
     8761 ggacatcgca aacaatatga agacggattt cttctctgcc gtgactcttt tcactgattg
     8821 cgtaactgtc atgctctgga ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt
     8881 tattaagccg cccctggttt ccttttaccg cgaataaata atcaccgccc tgtttttgta
     8941 tcttctctgc aatatctttc tggcatccca tcgcatcagt tgtgatgatt tttcctttaa
     9001 tatccagcat gttaagaagt tcaggaatgg ctgtaatctc attggatttc tcatccgtct
     9061 taatctgccc gagaaccaga ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc
     9121 ccttgcggcg actcttgtca taagagtgcc ggagcgtttt tccatcaatt gcaatgacgt
     9181 ctttatcatc tgaagaatgg cagtcacgca tccagttaat aaagcactcg tgaaattttg
     9241 caggactgat acaggataca actctggcaa tagtatcgtg aacaggaata ccattttcaa
     9301 aatcaccata ttgcttcaaa aaatcgagat gtgtttcacc aaaatcctct atatcttccc
     9361 aaccctctgc accagaaatg acggcacaaa tagtcaacag tagaatatct gacaatttat
     9421 gttctacttt ccaggcttgt ctgtaatcgg ggataataga aatatgttcc atcagttttt
     9481 taagttccat tttgttctcc ttatttatgc aaggagtatt tgatcatata taagctatga
     9541 aaaatagttt taggtaataa ggaatatctc aattattgaa catttagcgc gaattattta
     9601 gtacaaaaag tgtacttccg atgatctttc cctgctccaa agtcttctaa aatcaacaaa
     9661 gtagactctt aattatattt atcaacacaa tagtctatcc tattactatc ttgacaagag
     9721 agaaccgttt tttatttaaa tatatgatac acatcattaa atatataacc atactttttc
     9781 ataaggcaat ctaacttagc tcttaaatca ccaaagccca tcacaccata acatctaata
     9841 gccattgcca cacgtcacta tggatttatt tccacctgca attacttcgt cctaacttta
     9901 atttaatctc aagaagcatt aagatctcaa tagcgagctt aatacttctt gaataatatc
     9961 actcctgacc aaaatttttc cacactaatg catgagactt tgatttgtca cccagaaatt
    10021 tacttaaatc ttgccaaagt aaagaattgc tatcggtcgc agtgactatt gcctctataa
    10081 gccctgtagc tacaactgtg gccaattcat catcaagcga gaccatacca tcttcgacat
    10141 gtttaaatat tttttctctt tcaatatccc ccatttcata aaaatttttt acaatcgttt
    10201 ttccaacctg agagaacaac ataaccaccg gtggcgtttc tggatgccag tattcattag
    10261 ctaatgagac tgtctcagac aaaacagtgg aaaatgatgc cattttttct atataactag
    10321 caatatattg gttcattact tgcctcccaa agcattctgt aaatcctgaa gaacgttttc
    10381 tgctgctgct cgatcccctg gagatgctgt tggattctta tccaaccaat cctgtaaagc
    10441 ccttgtataa tcctgtgctt tcaatacatg tcctctacca ccaacttttt cacctgtggc
    10501 caattcataa cgaacagcat ctgctgtgct tccacttcct acttttgcat ttggccgata
    10561 aagatcgtct ataatatttt tcaaatttga atctgtaacg tcgggttttc ccattttctg
    10621 tcgcaattca tctttatacc gttcatgctg catacgatcc cttgcgctat tctcatcttc
    10681 tggtccccat cctcccgggc ttcccgtact gccgccggcc aagcagttct tctcttcatc
    10741 cgtcaaatcc ttcccaacat tcggctgact ccctagggaa agattacgaa agcccgctcc
    10801 ccgcaaggac tgacgcgaga tagtttctat ccatggctgc ttttcgcatc ttacgtctta
    10861 accctgcctt gaatacctta tcattcgtta aaatattaat agcgatgtgc cgtatccctg
    10921 aaaataattc tgcggcgttt cctcttctta ttttgcagtc gtcttcattc attaccacgt
    10981 ccagacgcca gtgcagctta ttctccacgt gccagtggtt tcggattgct gtggcgaact
    11041 tttctgcggt taaatcagca gaactgatat aatatctgac cgtcatttct ggctctttct
    11101 tttgttctgc tattattgac cgaaaggaga ctgccacgca taatttcttc agtcctttcc
    11161 attcaaacgt gaaatcaata agttcatcag ggacatcgca aacaatatga agacggattt
    11221 cttctctgcc gtgactcttt tcactcattg cgtaactgtc atgctctgga ttatttaatt
    11281 ctttcagcgg aaatttttcc tcaaaggctt tatttagccg cccctggttt ccttttaccg
    11341 cgaataaata atcacctccc tgtttttgta tcttctctgc aatatctttc tggcaaccca
    11401 tcgcatcagt tgtgatgatt tttcctttaa tatctagcat gttaagaagt tctgggatag
    11461 ctgtaatctc attagatttc tcatccgtct tgatctgtcc gatgaccaga ctgtgcattg
    11521 ttgagaacgc actaatgaca tgaatcgctc ccctgcggcg actcttgtca taagagtgcc
    11581 ggagcgtttt tccatcaatt gcaatgacgt ctttatcatc tgaagaatgg cagtcacgca
    11641 ttcagttaat aaagcactcg tgaaattttg caggactgat acaggataca actctggcaa
    11701 tggtatcgt
//
LOCUS       DAZPJR010000131        11497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPJR010000131 DAZPJR010000000
VERSION     DAZPJR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11497
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.132"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            106..777
                     /locus_tag="ACHVB7_003216"
     CDS             106..777
                     /locus_tag="ACHVB7_003216"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR1915125.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            787..1983
                     /locus_tag="ACHVB7_003217"
                     /pseudo
     CDS             787..1983
                     /locus_tag="ACHVB7_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            1983..2543
                     /locus_tag="ACHVB7_003218"
     CDS             1983..2543
                     /locus_tag="ACHVB7_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1915126.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2545..3318
                     /locus_tag="ACHVB7_003219"
     CDS             2545..3318
                     /locus_tag="ACHVB7_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR1915127.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3506..3781
                     /gene="frmR"
                     /locus_tag="ACHVB7_003220"
     CDS             3506..3781
                     /gene="frmR"
                     /locus_tag="ACHVB7_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR1915128.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3816..4925
                     /gene="frmA"
                     /locus_tag="ACHVB7_003221"
     CDS             3816..4925
                     /gene="frmA"
                     /locus_tag="ACHVB7_003221"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR1915129.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5018..5851
                     /gene="fghA"
                     /locus_tag="ACHVB7_003222"
     CDS             5018..5851
                     /gene="fghA"
                     /locus_tag="ACHVB7_003222"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR1915130.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6077..6616)
                     /locus_tag="ACHVB7_003223"
     CDS             complement(6077..6616)
                     /locus_tag="ACHVB7_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR1915131.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6718..7929)
                     /gene="mhpT"
                     /locus_tag="ACHVB7_003224"
     CDS             complement(6718..7929)
                     /gene="mhpT"
                     /locus_tag="ACHVB7_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR1915132.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8216..9229)
                     /gene="mhpE"
                     /locus_tag="ACHVB7_003225"
     CDS             complement(8216..9229)
                     /gene="mhpE"
                     /locus_tag="ACHVB7_003225"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR1915133.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9226..10176)
                     /gene="mhpF"
                     /locus_tag="ACHVB7_003226"
     CDS             complement(9226..10176)
                     /gene="mhpF"
                     /locus_tag="ACHVB7_003226"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR1915134.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10173..10982)
                     /gene="mhpD"
                     /locus_tag="ACHVB7_003227"
     CDS             complement(10173..10982)
                     /gene="mhpD"
                     /locus_tag="ACHVB7_003227"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR1915135.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10992..>11497)
                     /gene="mhpC"
                     /locus_tag="ACHVB7_003228"
     CDS             complement(10992..>11497)
                     /gene="mhpC"
                     /locus_tag="ACHVB7_003228"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR1915136.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2763 a         3103 c         2876 g         2755 t
ORIGIN      
        1 agcaacattt aacatttata aattaacatc tggctgcgcc agagattacg tctctgagtg
       61 catcgttgtg cttgtagtat aataaataaa atggagtgtc attaagtgga taaggtttta
      121 gattcagccc tcctttcttc ggcaaataaa agaaagggta tccttgctat tggcgcacat
      181 cctgacgata tagaattagg ctgtggcgca tcgcttgctc gtcttgcgca aaaaggaatt
      241 tatatcgcag cagtggtgat gactaccggc aactctggta cagatggaat aatcgatcgc
      301 catgaagaat cgcgcaacgc cctaaagata ttagggtgca accaaactat tcatcttaat
      361 tttgctgaca cccgcgctca tttacagctc aatgatatga tttccgccct ggaagacatc
      421 attaaaaatc aaattccttc tgatgttgaa atcatgcggg tatataccat gcatgatgcc
      481 gaccgccatc aggatcatct cgctgtttat caagcttcaa tggttgcctg ccgcactatt
      541 ccacaaattc tcggctacga aaccccaagt acctggcttt catttatgcc tcaggttttt
      601 gaatccgtta aagaagaata tttcacagtc aaacttgctg cattaaaaaa acataaaagc
      661 caggaacgac gtgattatat gcgtcatgat cgcctgcgtg cagttgcaca atttcgcggg
      721 caacaggtca atagcgatct gggtgaaggc tttgtaattc ataaaatgat tctttgagca
      781 aattgcatga aaacctggat atttatctgt atgtccatag caatgttgct atggttttta
      841 agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta ttgacgccat tatacctgcg
      901 tataacgaag gcccgtgtct ggcgcagtca ctggataatc tactgcgtaa cccttatttt
      961 tgccgggtaa tttgcgttaa cgacggctcc acggacaata ccgaagcggt catggcggaa
     1021 gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt aaaaaaatac cggtaaaggt
     1081 ggtgcgctga tgaatggcct caattatgcc acctgcgacc aggttttttt aagtgatgcc
     1141 gacacctatg ttccgcccga tcaagacgga atgggctata tgctggcaga aattaagcgc
     1201 ggtgctgatg ccgtaggcgg cattccctct actgcgttga aaggcgcggg gctgttaccg
     1261 cacatccgcg cgaccgtaaa gttgccgatg attgttatga agcgcacgct acagcagctc
     1321 ctgggcggcg caccgtttat tatcagcggt gcctgcggaa tgttccgtac tgatgtattg
     1381 cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc ttgatctcac ctggacatta
     1441 gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca tcgtataccc acaggaatgc
     1501 aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga ttgtgggcta cgcggtctgt
     1561 atgcgcctgc ataaaaggct tttatttagc cgcttcggta tcttcagtat atttcctatg
     1621 ctgttggttg tgatttatgg cgttgggatt tatctcacta cctggtttaa tgaattcatc
     1681 accaccgggc cgcatggtgt ggtgttggca atgtttccgc ttatctggat cggcgtagtt
     1741 tgtgttattg gtgcttttag cgcctggttt catcgttgct ggttgttggt gcctttagcg
     1801 ccgctttccg ttgtgtatgt attattagct tatgccatct ggattatcta tggacttatt
     1861 gcctttttta ctggacgcga acctcagcgc gacaaaccca cccgctattc cgcactggtg
     1921 gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa ctgaaaaact atctgaagct
     1981 taatgttaaa gagaaaaatt attcagattg cagaccaggt aattattgat gaaactgcag
     2041 gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg tgccgttatt cagggtccgg
     2101 tagtgattgg cgcaaactgc ctgataggta attatgcgtt tattcgtcct ggcacaataa
     2161 tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa aaatgcggtt attgaagcgg
     2221 aagcaacgat tggtccgcaa tgttatattg ccgactcggt agttgcaaac caggcatatt
     2281 tgggcgcaca agtacgtacc agtaatcatc gtctggatga acagcctgtg tctgttcgaa
     2341 ctccagaggg aattatcgct accggatgcg ataaattagg ttgttatatc gggcagcgtt
     2401 cacgccttgg cgtacaagtt attattttgc ctgggcgaat tatttccccg aacacgcaac
     2461 ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg aacttattca ctccgacaag
     2521 aacttatccg tacaggagat taaaatgata aaacggacgt tattagcggc ggccattttt
     2581 agcgcattgc ccgcttatgc cgggttaact tccattaccg cgggctacga ttttaccgat
     2641 tattctggcg atcatggcaa ccgtaattta gcgtatgctg aactggtggc gaaagttgaa
     2701 aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg attatgaaac tgaacatttc
     2761 aatgccactc gcggtcaggg tgcggtctgg tataaatgga ataactggct gacaacccga
     2821 acgggtattg cctttgcgga taatacgccg gtctttgccc gccaggattt tcgtcaggat
     2881 attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg gttatcgcta cactaaatat
     2941 tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat cactctatac tggcccggtg
     3001 atcaccagct accgctatac ccattatgac tccagcgatg caggtggtag ttatagcaat
     3061 atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt atacgcaact atggctaagc
     3121 cgcggaacag gcgcttacac ctatgactgg acgccagaaa cacgctacgg cagcatgaag
     3181 agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac ttaatctcgg gctgacggca
     3241 ggtaaagtgt ggtacgacac cccaaccgat gattataacg gtctgcaact tgcagcccat
     3301 ctgacctgga aattctgatt gcttctgccg cccgctatcc ggggcggcct tccctgccga
     3361 ttagccccac tccctttcct cgttgttttc cgaccacatt caccggataa attttattct
     3421 ccagcgttat atactatagg gaggtatgca ttgacatata gcatacctcc ctatagtata
     3481 ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg gaagagaaga aaaaggtcct
     3541 tactcgagtt cgtcgtattc gggggcagat tgatgctctg gaacggtcgc tggagggtga
     3601 tgccgaatgc cgtgccatac tccaacagat cgctgccgtt cggggcgcgg ctaacgggct
     3661 gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt gaccgaaatg actgctacag
     3721 ccgcgaagtc agccaatccg ttgacgacac tattgaactg gttcgagcct atcttaaata
     3781 gctgaatcta ttaccatatt gaggaagagc gagagatgaa atcacgtgct gccgttgcat
     3841 ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt tgcaccaccg aaaaaaggtg
     3901 aagtgctgat taaagtcacc cataccggcg tttgccatac cgacgcattt accctctccg
     3961 gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca cgaaggggcc ggcgttgtgg
     4021 ttgaagtcgg tgaaggcgta accagcgtca aacctggcga ccatgtgatc ccgctttaca
     4081 ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa aactaacctc tgtgttgcgg
     4141 ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac cacccgtttt tcttacaacg
     4201 ggcagccgct ttatcactac atggggtgct ctacattcag tgaatacacc gtggtcgcgg
     4261 aagtgtctct ggccaaaatt aatccagaag caaaccatga acacgtctgc ctgctgggct
     4321 gtggcgtgac caccggtatt ggcgcggtgc acaacacagc taaagtccag ccaggtgatt
     4381 ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt ggttcagggc gcgcgtcagg
     4441 cgaaagcggg acggattatc gctatcgata ccaacccgaa gaaattcgat ctggcgcgtc
     4501 gcttcggtgc taccgactgc attaacccga atgactacga caaaccgatt aaagatgtcc
     4561 tgctggatat caacaaatgg ggtatcgacc atacctttga atgcatcggt aacgtcaacg
     4621 tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg tcagtcggtg atcatcgggg
     4681 tggcaggttc tggtcaggaa atctccaccc gtccattcca gttggtcact ggtcgcgtat
     4741 ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca gttaccgggt atggttgaag
     4801 atgcgatgaa aggtgatatc gatctggaac cgtttgtcac gcataccatg agccttgatg
     4861 aaattaatga cgccttcgac ctgatgcatg aaggcaaatc cattcgaacc gtaattcgtt
     4921 actgatttcc cgcaggtata ccccgtccac ttcagacggg gttcttaata ctctccccgg
     4981 gcagccgtcc gggggattaa ccgtgagata atgactgatg gaactcattg aaaaacatgc
     5041 cagctttggc ggctggcaaa atgtgtatcg gcattattcc caatcactga aatgtgaaat
     5101 gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa aaattgccgg tgctgtactg
     5161 gctttcaggc ctgacctgca acgagcagaa tttcattact aaatcgggga tgcagcgtta
     5221 cgcggctgag cacaacatta ttgttgttgc gccggacacc agtccgcgag gcagtcatgt
     5281 cgcagatgcc gaccgttacg atctcgggca aggtgccggg ttttacctga acgcaacgca
     5341 agcgccgtgg aatgaacatt acaaaatgta tgactatatc cgcaacgagc tgccggattt
     5401 agtgatgcaa cattttccgg caacgaccag aaagtctatc tctggtcatt ctatgggcgg
     5461 gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa tatgtcagcg tctcggcgtt
     5521 ttcgcccatt gtctccccat cgcaagtacc gtggggacag caagcctttg ccgcatatct
     5581 tggtgaaaat aaagatgcct ggttggatta cgatccggtg agtcttattt cacaaggtca
     5641 acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat gatttttacg cggaacagct
     5701 gcggactcca aatcttgaaa agatctgcca ggagatgaat atcaagacgt taatccgtta
     5761 tcacgagggt tacgaccaca gctattattt tgtctccagt tttattggcg agcatattgc
     5821 ctaccacgcc aataaactga atatgcgttg ataatagtgc acgactgccg gatgcggcgt
     5881 gaacgcctta tccggcctac acttcgcccg taaaccgtag gcctgataag acgcgcaaag
     5941 cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt gaacgcctta tccggtctac
     6001 acttcgccca taaaccgtag gcctgataag acgcgcatag cgtcgcatca ggcatctgcg
     6061 cacgacttat ctatgtttac cacatcaaat catcaggcac tttgaagtcg gcatacggat
     6121 cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa cacaatactg ctggcatcac
     6181 gctgagcaat tttatcggca acactggcgg gaataatggt gtattcacct tcgctattgt
     6241 tatcgaccaa caagcgggca atcgccagac gaccattaat caactgcgcc tgtgtgagct
     6301 tatcgacaaa aatcttctta atcagattgc cgtcagtgaa gttaaaacca atatcgccat
     6361 tggcaatggt gattcggttc atttcaatga gctgcttcac ctgggcttta tattctttcg
     6421 ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc acgctcaagc tgtgcctttt
     6481 tattttcttc taccgcagcc cgagcttcac gcgcctgaac gcgcgatttt ttcgccgttc
     6541 tctcgacctt cgccgctttt ttgctggtga ctaatcctgc tttgagcaac tgctcttgta
     6601 aggtaagttt tgccatcttt gtttccaaac ccgctgaata atttttagcg agtatacctg
     6661 taaacgccgt gggtgtattg ggggcaagtc cctttcgccc ctgcttaata aggcgcgtca
     6721 ggcatcggca cacggctgca ttcttgatct ccgactcatc aaaataaaca ccgccaaccc
     6781 agcaacaaga atacccggtg cagaagcggc cattacgccg accgtgccag tgcctaatgc
     6841 cagcattttc ccggccagta acggaccgct catagccccc agacgcccta ccgccacggc
     6901 tgttcccaca cctgttgcgc ggatctgcga actgtaaaac aacagtgcca gggcatacaa
     6961 aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat cccgccagca acataccgtt
     7021 aaatgacgac accgttccaa gcgccagcag cgaagctaac atgccgctat aaatcagtag
     7081 cgacatggtt actggacgca gcttatccat caatgcgccc aacattaacg tcccgcttgc
     7141 cgcccccatt tgcagggcaa acatcacccc tgccgcctgc gatggctgga atccttgctc
     7201 caccaaaagt agcggtagcc agttgatcaa catataaacc accagcagag tgaagaaata
     7261 acacaaccac agcagcagcg tcgcggttgc cgtttctggc gcaaataagg cacgcagtgg
     7321 tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc ggcagccagc gcattaatag
     7381 cggcaccaga atcaacggca ccacaccacc tacccaaaac accgtttgcc atgctaagtt
     7441 tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca atgggaacac cgcaatacat
     7501 caggctcact gccgtcccac gaaaacgtgg acccgcggct tcagatgtca gggcgataag
     7561 attcggcaac gccgccccca gcccgacacc ggtcatcagc cgcgcaaaga ccagtgaggg
     7621 gaaatcccag gcaatcgccg ttgccagtga gaacaaacca aacagcgcaa ctgagccaat
     7681 caaaatacgc ttacgaccat aacggtctgc cagcatcccg ccaaccaacg cgccgggtag
     7741 caaaccgagt attccggcgc taaatatcca gcccatttgc attttatcga gtgcgaaagc
     7801 ctgggcgatg ccacccgccg caatgccagc cgcctgaaga tccagccctt ccatcagagc
     7861 gaccaaaaaa caaagcccga tggtcagcat caggcgggat gaagatgatg aaggggtacg
     7921 agtcgacata agatgaacct cattgcgagt gcaggtgaat cgcgactgat acattgccag
     7981 ccgcgtaatc agaactattt aatgactgcc tacgggcgaa atgtagcgaa atgtaggccg
     8041 gataaggcgt tcacgccgca tccggcagtc gtgcgcagat gcctgatgcg acgctaacgc
     8101 gtcttatcag acctacaaat tctcacgcca tccgtaggcc ggacaaggcg ttcacgccgc
     8161 atccggctgt cgtgctggga tgggcataca aaatgcacgt tatcggcagt catcattatt
     8221 tgttgttgcg cagatccagc gccacgtcga cgatcatatc ctcctggccg ccaaccatcc
     8281 ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa gccataacgc gccgccgccg
     8341 tttcacagtg acgcaggaag ctcgagtaaa caccagcgta tcccagcgcc agcgtttcgc
     8401 gatcgactcg taccggacga tcctgcaacg gacgcaccag gtcgtcggcg gcatccatta
     8461 acgcatagag atcggtccca tgctgccagc ccagtttatc cgcggcggca ataaacactt
     8521 ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag gctggcgtcg attcggtcgc
     8581 agccctcttc caccgccgcg atagagttcg ccacgccaag actcaggtta tggtgagcgt
     8641 gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag ggcgcggaaa cggtcacgga
     8701 tatcgctcat gttcatcgca ccgccagaat ccaccacata aatacaggtc gcaccgtagc
     8761 cttccatcag ctttgcctgc ttggcgagat tctccggcgt ggtcatatgg ctcatcatca
     8821 gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg aatatgctgg gcggaaacat
     8881 cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc gccagcctgc caggcatttt
     8941 tcagatcgtg aatagtgccg attcctggca gcaacaacgt cgcgattttg gcgtgcctca
     9001 ccacatccgc cgccgcttca atccattcaa ggtcgctatg tgcgccgaaa ccatagttaa
     9061 agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat cgaatccacg cgggcatcgt
     9121 ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata ctgatgacga atggcgtgca
     9181 taccgtcacg caatgtgacg tccgagatat aaagtttttt accgttcatg ccgcttctcc
     9241 tgccttgcgc gccagtgact gggccatttt ttccgctgtc gccagcgcac tggaagtcat
     9301 aatatcgagg ttgcccgcat aggcaggcag ataatgcgct gcgccttcga cttccagcca
     9361 gaccgctgtt ttcagtccgg agaattgccc cacgcccggt aaattgaccg gtttatcctg
     9421 cgggataact tcaaactgca cgcgctgttt caggcgataa cccggtacgt aagcctgcac
     9481 cgcctcagcc atttcattga ttgaggcttc gatatcatct tgtgaagctt cgtcgctcaa
     9541 tacatacacc gtgtcacgca tcatcaacgg tggctctgct gggttaagca caataatcgc
     9601 cttccctttt gccgcgccgc ccaccacttc aatggctcgg gaagtggttt ccgtaaattc
     9661 atcgatattg gcacgcgtgc caggtccggc agatttactg gcgatagaag cgataatttc
     9721 ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc attggaatgg tggcctggcc
     9781 gccgcaggtg accatgttga cgttcagttg atcgacgttc gcctcgaggt taaccaccgg
     9841 cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt aagcgaatat ccggtttcgc
     9901 ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg ctggtcgcat caaatacaat
     9961 gtcgatatca gcaaattcag gcatgttcat cagcccgatc accccttcat gggtggtggc
    10021 gacgcccata cgtctggcac gcgccagacc gtcggactga ggatcaatgc cgaccatcac
    10081 cgccatctcc agatgctgac cgtgacgcaa aattttaatc atcagatcgg tgccaatgtt
    10141 gccagaaccg ataatggcga ctttacgctt actcatgaca gacttccttt tggggctgcg
    10201 cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat gggcttcaaa acgatcgccc
    10261 gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa tgatatctcc ggcgcgcagc
    10321 ggttcaccca gactggccat tttgcgtgcc agccagacgg cggcattaag cggatgtccc
    10381 aggcattcgc tgccgcgccc gctggaaacc tcttcgttat tacgcgtcat cttcatggcg
    10441 cagtttttca ggtctaaccc cgccggacgt tgcgccggac cgccgatgac atacaccccg
    10501 caggaagcgt tatctgccac ggtatcgaca aactgaatcg accagtcgcg aatgcggctc
    10561 cccaccactt ccagcgccgg aagtacccat tcaatggcgt tatacagttc gtcgaaggtg
    10621 atatcagttg cgggtaaatc gcggttcaac accagtgcga tctccgcttc aatgcggggt
    10681 tgcagaacac gggaaaaagg aatgatttcg ttatcgccat aacacatgtc ggcaaataac
    10741 gtcccaaaat ccggttgatc aacgcccagt tgttgttgca ctttcggatg cgtcaggccc
    10801 actttacgcc ctaccacgcg acgcccctgc acaacgtcat attgcacatt tatgtgctga
    10861 atggcgtagg ccgcttcagc gtgatcgata ccaatcagat cgcgcagcgg cgcaatcgct
    10921 tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt gctcaagagt atgcttcgtc
    10981 atgaccattc cttaagggcg tgcgaggaaa ttcagcacca attgattgaa agcgtcggca
    11041 tgttcccact gcgcccagtg accacagtcg cggaagatat gcagttcaga accggcaatg
    11101 ccggacagca gacgcagacc cgcatccatc ggcacaaagc ggtcgttgcg cccccagaca
    11161 atcagggttt gcgctttgat ttccgccaga cgtgggccaa aatccgggaa ctgtttcgga
    11221 ttagcttcca ggctcttaac gaagttttcc aggtgatcgc ggcgcgacag catattattc
    11281 aggcgcgctt caaacagggc gtcggtcaaa tcgctggtat cgaaaacgaa gatatccatc
    11341 atcagcttca ggttttcgat agtcggctga cgataaagct gattcagtcg cttaatacct
    11401 tcggttggca tcggcgtaaa caaactcatg ccgcccgtac cgccgcccat cagcaccagt
    11461 ttgccgacgc gctccggcca gttaagggtg aacgcca
//
LOCUS       DAZPJR010000132        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPJR010000132 DAZPJR010000000
VERSION     DAZPJR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.133"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHVB7_003229"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHVB7_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR1915137.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHVB7_003230"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHVB7_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR1915138.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHVB7_003231"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHVB7_003231"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR1915139.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHVB7_003232"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHVB7_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR1915140.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHVB7_003233"
     CDS             complement(3928..5214)
                     /locus_tag="ACHVB7_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR1915141.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHVB7_003234"
     CDS             complement(5235..6026)
                     /locus_tag="ACHVB7_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR1915142.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHVB7_003235"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHVB7_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR1915143.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHVB7_003236"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHVB7_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR1915144.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHVB7_003237"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHVB7_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR1915145.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHVB7_003238"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHVB7_003238"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR1915146.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHVB7_003239"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHVB7_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR1915147.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHVB7_003240"
     CDS             complement(9882..10364)
                     /locus_tag="ACHVB7_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR1915148.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHVB7_003241"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHVB7_003241"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR1915149.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2803 a         2747 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZPJR010000133        11357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPJR010000133 DAZPJR010000000
VERSION     DAZPJR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.134"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..537)
                     /gene="espL1"
                     /locus_tag="ACHVB7_003242"
     CDS             complement(<1..537)
                     /gene="espL1"
                     /locus_tag="ACHVB7_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR1915150.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYA"
     gene            1062..2990
                     /gene="thrS"
                     /locus_tag="ACHVB7_003243"
     CDS             1062..2990
                     /gene="thrS"
                     /locus_tag="ACHVB7_003243"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR1915151.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2994..3536
                     /gene="infC"
                     /locus_tag="ACHVB7_003244"
     CDS             2994..3536
                     /gene="infC"
                     /locus_tag="ACHVB7_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR1915152.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3633..3830
                     /gene="rpmI"
                     /locus_tag="ACHVB7_003245"
     CDS             3633..3830
                     /gene="rpmI"
                     /locus_tag="ACHVB7_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR1915153.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3883..4239
                     /gene="rplT"
                     /locus_tag="ACHVB7_003246"
     CDS             3883..4239
                     /gene="rplT"
                     /locus_tag="ACHVB7_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR1915154.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4356..4480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4362..4406
                     /gene="pheM"
                     /locus_tag="ACHVB7_003247"
     CDS             4362..4406
                     /gene="pheM"
                     /locus_tag="ACHVB7_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR1915155.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4689..5672
                     /gene="pheS"
                     /locus_tag="ACHVB7_003248"
     CDS             4689..5672
                     /gene="pheS"
                     /locus_tag="ACHVB7_003248"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR1915156.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5687..8074
                     /gene="pheT"
                     /locus_tag="ACHVB7_003249"
     CDS             5687..8074
                     /gene="pheT"
                     /locus_tag="ACHVB7_003249"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR1915157.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8079..8378
                     /gene="ihfA"
                     /locus_tag="ACHVB7_003250"
     CDS             8079..8378
                     /gene="ihfA"
                     /locus_tag="ACHVB7_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR1915158.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8479..9459
                     /gene="btuC"
                     /locus_tag="ACHVB7_003251"
     CDS             8479..9459
                     /gene="btuC"
                     /locus_tag="ACHVB7_003251"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR1915159.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9522..10073
                     /gene="btuE"
                     /locus_tag="ACHVB7_003252"
     CDS             9522..10073
                     /gene="btuE"
                     /locus_tag="ACHVB7_003252"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR1915160.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10073..10822
                     /gene="btuD"
                     /locus_tag="ACHVB7_003253"
     CDS             10073..10822
                     /gene="btuD"
                     /locus_tag="ACHVB7_003253"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR1915161.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10900..>11357
                     /locus_tag="ACHVB7_003254"
     CDS             10900..>11357
                     /locus_tag="ACHVB7_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR1915162.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2873 a         2631 c         3057 g         2796 t
ORIGIN      
        1 ggcgtattgc aatgacagat ggcgacaaaa aatagcgtca gaaggagatt gcgaaaaaaa
       61 tgcactacca ttgagattta ctgttggttg tatagttttt tctctatgga tttttttaga
      121 cagtttcata caatggttac gatcttcggc agttaactgt tcctgttcaa tataatccag
      181 tatgcttcgc aaatggttag ccttaatagg cgtggtttcc agaaataagg gaatgatttc
      241 ttttttatag tgtaatgtaa tgagtatatt ttctgcatgg atgggtgaag tggcaaagtt
      301 aatgataaaa agggcagtgg aataacaatt ttgtgaaatt tttcgacagt ttgcggtaaa
      361 ttcctgagta acacagtatg acagcatcga acttaagtca gaggtatcaa gtagggtgat
      421 gagttggcgc gcaaattgat taataggctc tgatgaaggc gttggtatgt cgctatcgat
      481 gtgcaatcta tgaggagact tataatgggt tctgataatg atatcgtttt gactcataat
      541 ctattcgcct tatatatgtc tggaggtttt gaggatgatg attaattatt atcgccagaa
      601 cgaaatgtgc acgaaaaatt gctataagaa ttgtgtcact tttatcattg tgatatgacc
      661 acttcgtcat taatctcatg agcgtttaaa atactaccgc caaatcacgc aacgaacttt
      721 gctggattca gtttatgctg ctgtaaatcc gctcgcagta aacctttcag acgcaccgtg
      781 atgttatcag tggttcttta agcgttttgc tggtgtactc actacaaacg aattgcgaat
      841 cgaatcaatg tgaaacggaa aggtacaatc tccctttttt tagtgttaac atcgttcaac
      901 cgggtggaag gtgagaggtt aatgtctttc atttgtgtac ctgattgcga accaatttag
      961 catttgttgg ctagatggtt tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt
     1021 gatctttcgt gtgggtcacc actgcaaatt aaggatataa aatgcctgtt ataactcttc
     1081 ctgatggcag ccaacgccat tacgaccacg ctgtaagccc catggatgtt gcgctggaca
     1141 ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg
     1201 cttgcgatct gattgaaaac gacgcacaac tgtcgatcat taccgccaaa gacgaagaag
     1261 gtctggagat cattcgtcac tcctgtgcgc acctgttagg gcatgcgatt aaacaacttt
     1321 ggccgcatac caaaatggca atcggcccgg ttattgacaa cggtttttat tacgacgttg
     1381 atcttgaccg cacgttaacc caggaagatg tcgaagcact cgagaagcgg atgcatgagc
     1441 ttgctgagaa aaactacgac gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa
     1501 ctttcgccaa ccgtggggag agctacaaag tctccattct tgacgaaaac atcgcccatg
     1561 atgacaagcc aggtctgtac ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg
     1621 taccgaacat gcgtttctgc catcatttca aactaatgaa aacggcaggg gcttactggc
     1681 gtggcgacag caacaacaaa atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa
     1741 aagcacttaa cgcttacctg cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa
     1801 tcggtaaaca gctcgacctg taccatatgc aggaagaagc gccgggtatg gtattctggc
     1861 acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag
     1921 agtaccagta tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa
     1981 ccggtcactg ggacaactac aaagatgcaa tgttcaccac atcttctgag aaccgtgaat
     2041 actgcattaa gccgatgaac tgcccgggtc acgtacaaat tttcaaccag gggctgaagt
     2101 cttatcgcga tctgccgctg cgtatggccg agtttggtag ctgccaccgt aacgagccgt
     2161 caggttcgct gcatggcctg atgcgcgtgc gtggatttac ccaggatgac gcgcatatct
     2221 tctgtactga agaacaaatt cgcgatgaag ttaacggatg tatccgttta gtctatgata
     2281 tgtacagcac ttttggcttc gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac
     2341 gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag
     2401 aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg
     2461 aatttaccct gtatgactgc ctcgatcgtg catggcagtg cggtacagta cagctggact
     2521 tctccttgcc gtctcgtctg agcgcctcct atgtgggcga agacaacgag cgtaaggtac
     2581 cggtaatgat tcaccgcgca attctggggt cgatggaacg tttcatcggt atcctgaccg
     2641 aagaattcgc tggtttcttc ccgacctggc ttgcgccagt tcaggttgtt atcatgaata
     2701 ttaccgattc acagtctgaa tacgttaacg aattgacgca aaaactatca aatgcgggca
     2761 ttcgtgttaa agcagacttg agaaatgaga agattggctt taaaatccgc gagcacactt
     2821 tgcgtcgcgt cccatatatg ttggtctgtg gtgataaaga ggtggaatca ggcaaagttg
     2881 ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga
     2941 agctgcaaca agagattcgc agccgcagtc ttaaacaatt ggaggaataa ggtattaaag
     3001 gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg
     3061 aagttcgctt aacaggtctg gaaggcgagc agcttggtat tgtgagtctg agagaagctc
     3121 tggagaaagc agaagaagcc ggagtagact tagtcgagat cagccctaac gccgagccgc
     3181 cggtttgtcg tataatggat tacggcaaat tcctctatga aaagagcaag tcttctaagg
     3241 aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat taaattccgt cctggtacag
     3301 atgaaggcga ctatcaggta aaactccgca gcctgattcg ctttctcgaa gagggtgata
     3361 aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg
     3421 aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc agtggtcgaa tccttcccaa
     3481 cgaagatcga aggccgccag atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc
     3541 ttcaagtagc actgtctgtg gagccttcgg gcttcacagg ctttgttcgc ctttgtttcg
     3601 tttattaaca atgcgaagtg gaagttatta aaatgccaaa aattaagacc gtacgcggtg
     3661 ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa gcacaagcac gctaacctgc
     3721 gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg
     3781 tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg
     3841 tttttaactt tttaattaga atatagatac aggagagcac atatggctcg cgtaaaacgt
     3901 ggtgttattg cacgcgcacg tcacaagaaa attttgaaac aagctaaagg ctactacggt
     3961 gcgcgttctc gcgtataccg cgttgccttc caggctgtta tcaaagctgg tcagtatgct
     4021 taccgtgacc gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac
     4081 gcagcagcac gtcagaacgg tatttcttac agcaaattca tcaatggcct gaaaaaagcc
     4141 tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag tattcgacaa agtagcgttc
     4201 accgctctgg ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa gagggagcta
     4261 gtctccctct tttcgtttca acgccatcaa aacattgact tttatcgccg tagccttttc
     4321 aataaaggtc ttttgaagag taccaaaagg taacgcaagc aatgaatgct gctattttcc
     4381 gcttcttttt ttactttagc acctgaatcc aggaggctag cgcgtgagaa gagaaacgga
     4441 aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa
     4501 aaagccagtc tatcaggcgt aattgtcgca gccagtctgg acacggccag cgcggagaaa
     4561 ccggagcgta tttaagtacg tgagaatttc gagcactgcc cgggaccaaa atggcaagta
     4621 aaatagcctg atgggatagg ctctaagtcc aacgaaccag tgtcaccact gacacaatga
     4681 ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc attagccagg
     4741 cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa aaagggcact
     4801 taacccttca gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg
     4861 cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac
     4921 tggaaagcgc agcgctgaat gcgcgtctgg cggcggaaac gattgatgtc tccctgctag
     4981 gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg taccatcgac cgtatcgaaa
     5041 gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc
     5101 ataacttcga tgctctgaac attcctggtc accacccggc gcgcgctgac cacgacactt
     5161 tctggtttga cactacccgc ctgctgcgta cccagacctc tggcgtacag atccgcacca
     5221 tgaaagccca gcagccaccg attcgtatca tcgcgcctgg ccgcgtttat cgtaacgact
     5281 acgaccagac tcacacgccg atgttccatc agatggaagg tctgattgtt gataccaaca
     5341 tcagctttac caacctgaaa ggcactctgc acgatttcct gcgtaacttc tttgaggaag
     5401 atttgcagat tcgcttccgt ccttcctact tcccgttcac cgagccttct gcagaagttg
     5461 acgtcatggg taaaaacggt aaatggctgg aagtgctggg ttgcgggatg gtgcatccga
     5521 acgtgctgcg taacgttggc atcgacccgg aagtttactc tggtttcgcc ttcgggatgg
     5581 ggatggagcg tctgactatg ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa
     5641 acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa tagattatga aattcagtga
     5701 actgtggtta cgcgaatggg tgaacccggc gattgatagc gatgcgctgg cgaatcaaat
     5761 cactatggcg ggcctggaag ttgacggtgt agaaccggtt gccggtagct tccacggcgt
     5821 ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac
     5881 aaaagtgaat gtcggcggcg atcgcctgct ggacatcgtc tgtggtgcgc caaactgccg
     5941 tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt ctgccgggtg atttcaaaat
     6001 taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg ctgtgctcct tctctgaact
     6061 gggaatttct gacgatcaca gcggcattat cgaactgcct gcagatgcgc cgattggcac
     6121 tgacatccgc gaatacctga aactcgatga caacaccatc gaaatcagcg taacgccaaa
     6181 ccgtgccgac tgcttaggta tcattggtgt tgcgcgtgac gttgccgtgc tgaatcagct
     6241 gccgctggtt gaaccggaaa tcgttccggt tggtgcgacc atcgacgaca cgttgccgat
     6301 tacagtcgaa gcgcctgaag cctgcccgcg ctatcttggc cgtgtggtaa aaggcattaa
     6361 cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc
     6421 tatcgatgca gttgttgacg tcaccaacta tgtactgctc gaactgggcc agccgatgca
     6481 cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg cggatggcaa aagagggcga
     6541 aacgctggta ctgcttgatg gcactgaagc gaagctgaat gctgacactc tggtcatcgc
     6601 cgaccacaac aaggcgctgg cgatgggcgg catctttggt ggcgaacact ctggcgtgaa
     6661 tgacgaaacg caaaacgtct tgctggaatg cgctttcttc agcccgctgt ctatcaccgg
     6721 tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac cgttatgagc gtggcgttga
     6781 tccggcactg cagcacaaag cgatggaacg tgcgacccgt ctgctgatcg acatctgcgg
     6841 tggtgaggct ggtccggtaa ttgatatcac caacgaagca acgctgccga agcgtgcaac
     6901 catcacttta cgtcgtagca aactggatcg cctgatcggc catcatattg ctgatgaaca
     6961 ggtaactgac attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag acgagtggca
     7021 ggcagttgcg ccgagctggc gtttcgatat ggagattgaa gaagatctgg ttgaagaagt
     7081 cgcgcgtgtt tacggctaca acaacatccc ggatgagccg gtacaggcaa gcctgattat
     7141 gggtactcac cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa
     7201 aggctatcag gaagtgatca cctacagctt cgttgatccg aaagtgcagc agatgatcca
     7261 tccaggcgtt gaagccttac tgctgccaag cccgatctct gttgaaatgt cagcaatgcg
     7321 tctttctctg tggaccggcc tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa
     7381 ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca gatactcagg caccgttggg
     7441 cattcgtcag gatctgatgt tagccggtgt gatttgcggt aaccgttacg aagagcactg
     7501 gaacctggca aaagagaccg ttgatttcta tgatttgaaa ggcgatcttg aatccgttct
     7561 cgacctgacc ggtaaactga atgaggttga gttccgtgca gaagcgaatc cggcactgca
     7621 tccggggcaa tccgcagcga tttatctgaa aggggaacgt attggttttg ttggagttgt
     7681 tcatcctgaa ctggaacgta aactggatct taacggtcgc actctggtgt tcgaactgga
     7741 gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc
     7801 gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg atattttatc
     7861 cgaatgtaag aaagttggcg taaatcaggt agttggcgta aacttatttg acgtgtaccg
     7921 cggtaagggt gttgcggagg ggtataagag cctcgccata agcctgatcc tgcaagatac
     7981 cagccgtaca ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg tagaggcatt
     8041 aaaagagcga ttccaggcat cattgaggga ttgaacctat ggcgcttaca aaagctgaaa
     8101 tgtcagaata tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa gaactggttg
     8161 aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg
     8221 gttttggtaa cttcgatctg cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg
     8281 gcgaggatat tcccattaca gcacggcgcg tggtgacctt cagacccggg cagaagttaa
     8341 aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc tgatctaact aaaaaggccg
     8401 ctctgcggcc ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac
     8461 ttcagcagaa cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc
     8521 tggttattat gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt
     8581 gaacaatgga tttcgccagg tgactggttt actcctcgtg gcgaactgtt cgtctggcaa
     8641 attcgcctgc cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc
     8701 gctgtaatgc aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct
     8761 aacggcgcag gcgtggggct tatcgccgcg gtattgcttg ggcaagggca actccccaac
     8821 tgggcgctag ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt
     8881 ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt
     8941 atctgtagcg cactaatgac gtgggctatc tacttttcca cctctgttga tttacgtcag
     9001 ctgatgtact ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg
     9061 ctggcattga tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta
     9121 gcacttggcg agatctcggc gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg
     9181 ctggtggcag cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc
     9241 tttattggtc tggtgatccc acatattctt cggttgtgtg gtttaaccga tcatcgcgta
     9301 ttacttcccg gctgcgcgct ggcaggggcg agcgcagtgc tgctggccga tattgtagcg
     9361 cgcctggcat tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg
     9421 ccggtgttta tctggttatt gttaaaagca ggacgttagc cgcaaaaaga cggtctatga
     9481 ttaaaagcta agatttttac tgaccacacc caggagaaac gatgcaagat tccattctga
     9541 cgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac gccggtaatg
     9601 tgctgttgat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg
     9661 agaatattca gaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc
     9721 agtttctgga acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat
     9781 ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa cggcgaagga cgccatccgc
     9841 tgtatcaaaa attgattgcc gcagcgccga ccgcagtcgc gccggaagag agcggattct
     9901 atgcccgtat ggtcagcaaa ggtcgtgcac cgctgtaccc tgatgatatt ttatggaatt
     9961 ttgagaaatt tttagttggc agggacggat tggtcatcca gcgtttttcc ccggatatga
    10021 cgccggaaaa ccccattgtg atggaaagca ttaaactggc gttggcaaaa taatgtctat
    10081 tgtgatgcag ttacaggatg ttgcggaatc tacccgccta gggccacttt ctggcgaggt
    10141 tcgggctggg gagatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttact
    10201 ggcacgaatg gccggaatga ccagcggtaa gggaagtatt cagttcgcgg ggcaaccact
    10261 ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac aacagcagac
    10321 gccgccgttt gcaatgccgg tctggcacta tctgacactg catcagcacg ataaaacgcg
    10381 taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggacgtag
    10441 caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgctgca
    10501 aatcacacca caagccaatc ccgcaggcca attgctgctt cttgatgagc cgatgaacag
    10561 tcttgatgtt gcgcaacaaa gtgcgttaga caaaattctg agcgctctgt gtcagcaagg
    10621 actggcgatt gtgatgagca gtcacgatct caaccacaca ttgcgtcatg cgcatcgggc
    10681 gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agagaagagg tgctcacgcc
    10741 gccaaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag
    10801 aatgctgatt tcgaccatct gatttcatta gtatcttgaa aatcgggtaa ttacgacgct
    10861 aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg
    10921 atcacagcac tgtttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga
    10981 ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc
    11041 acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt
    11101 gtaacgatgc gcgatcgttt cgatttgcag ctgccccgag aaaccaaaga acaagcctct
    11161 atcggcacgc aaattgataa agacgagttg ctgcctggtg acctggtctt tttcaaaacg
    11221 ggttccggac aaaatggttt gcatgtaggt atttacgata ccaacaacca atttatccac
    11281 gcctctacca gcaagggagt gatgcgttcc tcacttgata atgtctattg gcagaaaaat
    11341 ttctggcagg cgagacg
//
LOCUS       DAZPJR010000134        11307 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPJR010000134 DAZPJR010000000
VERSION     DAZPJR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.135"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHVB7_003255"
     CDS             <1..208
                     /locus_tag="ACHVB7_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR1915163.1"
                     /translation="IVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFA
                     QTTKGVLETGIYRGVVSIYLSQDI"
     gene            300..2984
                     /locus_tag="ACHVB7_003256"
     CDS             300..2984
                     /locus_tag="ACHVB7_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR1915164.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2981..4069
                     /locus_tag="ACHVB7_003257"
     CDS             2981..4069
                     /locus_tag="ACHVB7_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR1915165.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="ACHVB7_003258"
                     /pseudo
     CDS             complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="ACHVB7_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5931..7343
                     /gene="uxaC"
                     /locus_tag="ACHVB7_003259"
                     /pseudo
     CDS             5931..7343
                     /gene="uxaC"
                     /locus_tag="ACHVB7_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            7358..8845
                     /gene="uxaA"
                     /locus_tag="ACHVB7_003260"
     CDS             7358..8845
                     /gene="uxaA"
                     /locus_tag="ACHVB7_003260"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR1915166.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8928..9479
                     /locus_tag="ACHVB7_003261"
     CDS             8928..9479
                     /locus_tag="ACHVB7_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR1915167.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="ACHVB7_003262"
     CDS             complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="ACHVB7_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR1915168.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2968 a         2772 c         2935 g         2632 t
ORIGIN      
        1 gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac
       61 ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa
      121 cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt
      181 aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg
      241 aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca
      301 tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat
      361 taaccgaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg
      421 tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa
      481 catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa
      541 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcaccacggt gaattggctt
      601 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
      661 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
      721 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
      781 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
      841 gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg
      901 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
      961 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
     1021 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
     1081 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
     1141 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
     1201 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg
     1261 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
     1321 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
     1381 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
     1441 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     1501 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     1561 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     1621 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     1681 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     1741 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     1801 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     1861 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     1921 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     1981 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     2041 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     2101 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     2161 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     2221 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     2281 cggcctttac gggcacttat agctctacgc tggctgtcag ttgctatggc gttaatttgg
     2341 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     2401 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     2461 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     2521 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     2581 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     2641 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     2701 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggaggattt acacttgaaa
     2761 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     2821 tgagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     2881 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     2941 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     3001 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     3061 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     3121 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     3181 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     3241 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     3301 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     3361 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     3421 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     3481 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     3541 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     3601 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     3661 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     3721 agatgaaggg cgaccagctg cagggcgcag agacggtgac ttctctatct atcgtcaagg
     3781 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     3841 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     3901 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     3961 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     4021 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattgac cccgcaggcc
     4081 agaaaaaact aaacaacgaa gccgcctgat aaggcggctt cgtcattaca acttacgtta
     4141 aacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     4201 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     4261 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     4321 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     4381 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     4441 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     4501 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     4561 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     4621 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     4681 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg
     4741 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     4801 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     4861 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     4921 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     4981 gccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc
     5041 ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg
     5101 ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc
     5161 tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac
     5221 cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg accgtataag
     5281 cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca
     5341 tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga
     5401 gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca
     5461 tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag
     5521 ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt
     5581 gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat
     5641 ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa
     5701 aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag
     5761 gccagtactg gcgcggcctg cagcgagatt tatcactttg agagtaattt tttaactacg
     5821 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     5881 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     5941 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     6001 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     6061 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     6121 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcatct gaccgtgaaa
     6181 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     6241 cgcaccttga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     6301 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     6361 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     6421 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     6481 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     6541 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     6601 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     6661 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     6721 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     6781 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     6841 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     6901 gtccgatggc ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     6961 cgaaaaccat tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg
     7021 gtaacttcta gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     7081 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga
     7141 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     7201 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     7261 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     7321 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     7381 gctggataac gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga
     7441 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     7501 tatcgcaaaa ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga
     7561 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     7621 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     7681 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     7741 aaccgtgggc tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac
     7801 caacaacgcc gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     7861 actgggcgac gatcacatca acatccgcac catgctgcaa aacatggtgc gccacccgaa
     7921 cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg
     7981 tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     8041 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     8101 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     8161 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     8221 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     8281 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     8341 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     8401 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     8461 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgcctg ggctgaactt
     8521 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     8581 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     8641 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg
     8701 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     8761 tgagtttgcc aacggtaagc aaacttgcaa cgagcgtaac gacttccgcg aactggcgat
     8821 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     8881 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     8941 ggctggccca gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc
     9001 gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     9061 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     9121 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     9181 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     9241 tcagtacctg ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     9301 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     9361 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     9421 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     9481 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     9541 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     9601 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     9661 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     9721 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     9781 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     9841 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     9901 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     9961 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
    10021 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
    10081 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
    10141 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacat
    10201 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
    10261 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
    10321 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
    10381 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
    10441 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
    10501 cccatgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
    10561 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
    10621 tgatatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
    10681 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
    10741 tcattgtgtt atcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
    10801 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
    10861 tttaaagaga agtcatattt tgaaacttaa tggttacatt tgtttgacat aaacagaggg
    10921 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
    10981 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag
    11041 ggggcggcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct
    11101 gcgcgattcc ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg
    11161 taagatgcgc cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg
    11221 cgattttgac gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccgattaca
    11281 taattaatag ctcacctttg tctggaa
//
LOCUS       DAZPJR010000135        11297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPJR010000135 DAZPJR010000000
VERSION     DAZPJR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.136"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..595)
                     /locus_tag="ACHVB7_003263"
     CDS             complement(<1..595)
                     /locus_tag="ACHVB7_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915169.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
     gene            complement(1025..1348)
                     /locus_tag="ACHVB7_003264"
     CDS             complement(1025..1348)
                     /locus_tag="ACHVB7_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915170.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            complement(1516..1935)
                     /locus_tag="ACHVB7_003265"
     CDS             complement(1516..1935)
                     /locus_tag="ACHVB7_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915171.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            complement(1935..2225)
                     /locus_tag="ACHVB7_003266"
     CDS             complement(1935..2225)
                     /locus_tag="ACHVB7_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915172.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            complement(2546..2836)
                     /locus_tag="ACHVB7_003267"
     CDS             complement(2546..2836)
                     /locus_tag="ACHVB7_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915173.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            complement(2836..3725)
                     /locus_tag="ACHVB7_003268"
                     /pseudo
     CDS             complement(2836..3725)
                     /locus_tag="ACHVB7_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4089..4751)
                     /locus_tag="ACHVB7_003269"
     CDS             complement(4089..4751)
                     /locus_tag="ACHVB7_003269"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR1915174.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            complement(5120..5671)
                     /locus_tag="ACHVB7_003270"
     CDS             complement(5120..5671)
                     /locus_tag="ACHVB7_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1915175.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            6258..6605
                     /locus_tag="ACHVB7_003271"
                     /pseudo
     CDS             6258..6605
                     /locus_tag="ACHVB7_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            6658..7053
                     /locus_tag="ACHVB7_003272"
     CDS             6658..7053
                     /locus_tag="ACHVB7_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR1915176.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            7094..7837
                     /gene="cmoA"
                     /locus_tag="ACHVB7_003273"
     CDS             7094..7837
                     /gene="cmoA"
                     /locus_tag="ACHVB7_003273"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR1915177.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7834..8805
                     /gene="cmoB"
                     /locus_tag="ACHVB7_003274"
     CDS             7834..8805
                     /gene="cmoB"
                     /locus_tag="ACHVB7_003274"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR1915178.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8970..>11297)
                     /gene="torZ"
                     /locus_tag="ACHVB7_003275"
     CDS             complement(8970..>11297)
                     /gene="torZ"
                     /locus_tag="ACHVB7_003275"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR1915179.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         2909 a         2629 c         2363 g         3396 t
ORIGIN      
        1 tcagcttagc cagtcttgat ttcagcaaat taatctcatt ttctcttgct ttcatcagtt
       61 tctctttggc aatttcttca agctcagctt gtgatttatc agcaacaatt agagacagtc
      121 gatgtggtgc tctgacagct ctttctgtac gaacaactct ttgaccttct ggtaaatctg
      181 caatcacttt accaatctct acagggtcat acccccaacc gtcacggtct agtcgagaga
      241 aataagcata agaattggtt tgaagctcca tcgaaataac catttcgtca atttcgtctt
      301 caagcttgga ctgaattttc tcgtaaacac ttctcattgc ttcttgagag tctttaatag
      361 cttggaaata tcggcgttgt agcttcaact tcaaatcaag ttcacaccgt tcgttcatag
      421 aaaaatcaat ttcaactctt ggaatctcga gtggttctgg tggaatatat ggacctttat
      481 ttacttcgtg tgattcccgc caagctctat caaaagattc acgcaaagtt aattcgtgaa
      541 tatcaagtgc acttggtctg tgttcgataa gcatttcatg ttcatttcta atcattcgtt
      601 caccccttag tttatttgtt atttcaattt atagaagcac actcagcaat cagaggtgaa
      661 gagtctgact gaacaagaac ctgccaagtg tgcttttaaa aactgaaaaa gccctaaaca
      721 tttctgtgaa gggcaaagga ttcaataatg aatgaacaag agtacttttt gagatagcct
      781 ctcagactaa cctgaaaggc catgacgaaa agcattgcac ctttccaact cagaatatcc
      841 atccatcgga ttattgaaag aaaaataaga gagaattgct ctcccataat taaaattatg
      901 ccatgcttaa ggttatttgt caacagcttt ttaacaggtt gataacatca attcttattt
      961 accgcattgt taataataca accaacaaat aagccaagca acgtccaagc aattattaaa
     1021 cccattagtg aaccctcaca gaatccgtca ccagaatatt atcactgtac aggattgtca
     1081 cagcaccttt caaaaattgc tctggggaaa tactgtatat gttcgtttgc tcgataacca
     1141 atcttttcat gttgctgtct gtaattggta gcatcattcg aggctcgtta aactccaccg
     1201 actgaataaa gtcagagatg ttttctacat tatcagccat cagctttagt aatttatcaa
     1261 aattaattgc gatacctgca tcaatgaagc tttgcaccat catagcagtg tcgttatcaa
     1321 ccataatttg cattgggtaa ttattcattt ttaaatctcc tatgaaacac acctcagacc
     1381 tcttaaaata gccattctga ggccagtaac catgcgggtt acagagggag ttgcactggc
     1441 tggtaaggta atttatcaaa ttaaggtgca actctgcctg taaagtgcat tctaccgttt
     1501 cttagatggt tatttttaat tgtctttatt aatcaatggc ttaatgtaga aatactgtct
     1561 ccgtgaaata ccatattttt ctgtaatgta tcgctgagat tgaccagcat ttatggcctg
     1621 agtgatatca tcacggttat agcggttacc acgggattta tatttgtcac catccacatc
     1681 ttctgaggta ttgaagtctt caatcagctt aattgcatcg tgaaggtcat cttctgaggc
     1741 ataatcgaag gtatggataa tctcaaaatc accagtatct gctggtgcat tcttccatct
     1801 ggagaagcgg gattttaatt gctggtgtgt gaagggttca gcattacgag ttaaatcttt
     1861 ccatgttccg ttattctcac ggacaaggtg acctctatgg cgataaaacg catgataatc
     1921 aaatgtgcat agcattaaac ccccagctta tccgccagtt gatgaacctt ggcactcaac
     1981 acctgaatca ttgagcaacc tgccactgcg gactgtgctt tgagtagttc cagttggccc
     2041 gttgtgattc tgatggcctt agaaccctct gcaacgccct tgatagcgtc aaacggtgta
     2101 agggctatgt gttccttcaa aatctcacca acaagtgctg tgtggctcat ggctgggtat
     2161 ttgtctttga ttgctgccag tctggaatat gcctctggca aaaggcgtgt aactgtgtgt
     2221 ctcattaaat tttccttctt aaaatctttg acttataact aactttaaca tatttataac
     2281 atattgtcaa caactcattt acatgttaat atggttgtag ggggctgata caggtcattc
     2341 cctttgataa ataaggggaa ttaatgggct ttcttgaaac aattgctgaa tctcacagat
     2401 tcgcaagctt aggttgcttt gtgtttctgg tgcttcttgt gtatcagctt ttaaaaatga
     2461 ttatcgccag aagtacacga ttcaatttca gcattaagct tttcaaatat gaaatcacaa
     2521 ttacgattga tgatggtaaa acaaactaac gttttgtgat aatattggtg gcctttaagt
     2581 tgctggggtc accgtctttt aatcgtagct ggtggattga attccagata tcaaaatgat
     2641 acagcactgc aaacctcaaa gcgtcaatct ttaaatctgt cacaccatcg taaaagaaat
     2701 aacccctccc ttctttcttt ggtctaatcc ggtaaacttc cccgtgagta tccttcataa
     2761 gatgaccttc acgagaaatg ctgtaacgct ttaacaggta atctttgtat ctcttttcac
     2821 cgtgatattc tttcattact taattcctcc accggcaaaa tacatcttcg gtttaatttt
     2881 ttgcttcttg tcatcctgag tttgtttctt tttcggtgaa agataataat catcttcact
     2941 gtcatcgaat gggtctaaac ccgatacttt gttcttttgt cttgcaagct cgaatctctt
     3001 tttcttctct gccctcttaa tggctttttc acgttcatcc tcttcatcat cgaaggggtc
     3061 caactcatca tcagtggcgg ggcactgtgt gtttttatgg tcactatcaa cactcacatc
     3121 agtgatttct ctgtcttgtt tagaagtctt tttagagctt acttcaaggt cagattcatt
     3181 catagagaga aaatcattta attcatccaa agaaagaaag tcatcttctt taatatcatc
     3241 attaagtcca gtctctttat tcttatctgt aatattatta tctaatgttt tatctaataa
     3301 cttatctaaa ctctaacacg ggtgacattt tgtcactacc cccatgacat tttgtcactg
     3361 gtagtggtgt cattttgtct tactgaggct gcaacttcct gtgatgtagg tcttacaagt
     3421 aattccggct tatcatccag tgtgaataca cgataagtgc tttgacgccc ctgattcttt
     3481 tgcacctgaa ttaaaccgtg tttctcaagt gtctcaatgg ctctgattgc tgttctcttt
     3541 gatacaccag ccatataagc gagatggtca atacttggtg aatatgattt gtatttctct
     3601 tcatagaagt cacagctatg gaccatgacc atataaatca atcttgctgc tgcgttaatt
     3661 tcttctgaaa gttgagagat tcgcacatca aatttataaa actgatttgg gttatattta
     3721 gacattcata aattacctca tacgtgaaaa ggccaaccgg ataaaaccga ttagcctgtg
     3781 tgattaaaat atctggggga ttgatggact gataaagaag tccatgcctg aaacagtacg
     3841 atgattaaaa cgctgaggta taccctgaat gagaggttaa tatacgccaa ccatctgaca
     3901 cctccccaag tgttaccact tcaagacatg aactttttta tcagcccata tagaaactat
     3961 aacagatgcg attataaatt gcaacaatta tttcacagaa tgattactga ttggtcattt
     4021 attgttcaca gggatacttc ttaacatctg aatcacctgt cttgtaaatg accatattta
     4081 tgagaatttt agctcaacct ttgagcaatc ctgtatgacg tgctcagagg cattctgaga
     4141 gcatctgcaa tgtacttaat gggttttcct tgcctgtata gttttatgaa gctgtcgagc
     4201 gtctcaggtg ggtaaatggt gggtcttcct cttagtccac cacggttaat gatggttaac
     4261 ggtagcaatg tatcagcaca gcaaaccatc tgtgtaattt catgcttacg tggttctttt
     4321 ttaaaaacaa cgactgcact tggaaacggt gacctaatag taagcctgtt atgccctgct
     4381 ggtggaaggt atttaactgc acgatgaaga atacggattt catgacagtg gttattgctg
     4441 taaatgtgcc taagccagta gctggtgttg gctctatgcg gtatcaggaa agctactaaa
     4501 tccgcttcag acgcttttaa gaggaacgac ggaatatcgc tgtacggcgg gttacagaag
     4561 accttttcac taacccatga ttgacggctt atatcatcgg tgaatcgtgg taacagcgtg
     4621 tcatgctcag ttgcagcacc atctagactg aaaataaatt cacggtgtaa gttcttaaat
     4681 aacaaaaatg gagttctcca gtaatcagaa cccccatgat gcttcccgat taaaatatgc
     4741 tcaattgaca taagaaataa aaatttttat tgcaatcact cttgacaagg tttactctct
     4801 tttaagagat gcttaacagg ttttctaaac ggatatcctt tctgggatat ctatctttta
     4861 tcttaaaagt ttttacagat actttaaaga tatctgtata tattctgtac agtaactttt
     4921 cagagtctga aaatttttat aaaaaatgac tagccctctt tgaatctcat cttccccgaa
     4981 tcttcgaaaa tatctgtcta gttgattcgg tagaatctac ctcatgatgt atacgtgttt
     5041 agtacccgtc acaccagcta tgtgaccccc ttggcaaaaa agccaaatat cagctacatc
     5101 catgtaacta ttcagtaact tatttagcaa gtctgtaata agttgctcta gagattccta
     5161 gctcttcctg aatctgcaac ctcgttttac cttgtgaaat aagcatctgt acagcctctg
     5221 aagaagcttt tacaggtctt cccatatgta ccccttcagc tttagcacgt tcaataccag
     5281 cctttctacg ctctgctatc aagcttcttt caagctgtgc caacgttgac ataatgccta
     5341 acattgcttc acccatagcg cttgataagt caaatccttc tctaaggctg ataactgtca
     5401 cacctttagc ctgtagcgct ttaaccgttg ataacacatc aattgtgtca cgtccaagac
     5461 ggtctacagc taccacaact acggtgtcac cttctcgtgc ataagctaac aaactgctga
     5521 aaccgtttct taaagaagct ttgacagaac cactgacagc ttcatcagcg aaccatttag
     5581 caatgttgta ccctgcctct tcaatttgca tttgctggtt agtgatggat tggtcatctg
     5641 tactcaccct gcaatatcca agtatcgcca taatgttccc cttagaagtc tcaaaagtta
     5701 gtctcataag cttagctgtc tcataatcca aaaacaacct tttgagacta cttgagggtg
     5761 cattctaccg ttacgttgaa agtgtctcaa aaggacttcc ttatgagact ggttaataga
     5821 gcgctatgaa gattcaggag ggtaactata accatcactt tctaactttc acgtaggaaa
     5881 tcgttaagtt tcttgatagt tacaccatgt gaccaactat ctatatatcg ttatatagcc
     5941 ctgtatatag cactatatgt agtgtctgga gagggacgac taacacgata cagtttaccc
     6001 tgaaaagaga cttcacagat tctttatata tttcaataag ttatgctaaa aagagactat
     6061 accgatactg catagaattt gtgtaggatt gcatagatac ttggaaaaac accgttaaat
     6121 tgcatagtca ttcacctgat attgtcctgt ttttacaggt agttgattcg gatgttctag
     6181 gaatcatcct catagttgat tcggttagtt gggcttgtac caacccctaa tcaacctcat
     6241 agtgtattta gaccttgatg attttagaca gccgccctgt tcatgcagca cgtccacaca
     6301 gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt tccggtacat gctgtactga
     6361 cggcgacaaa tccgctgatt cgttttatcg gtagtgatga tatgacgcaa aaccgggaat
     6421 tatttcaggt ctggttacag aaattagcgc agtggcatca gaccactacg ccttatcttt
     6481 ttttacatac gccagacatt gcccaggccc cggaactggt acataccctg tgggaagact
     6541 tacgtaaaac gcttccagag atcggagcag ttccggctat tccacagcaa tcttctcttt
     6601 tctgaatttg ccacctatca tagacaggtg ccatcggcca ttttaaaggg agtttgtatg
     6661 gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa tgaagttctc ttttgatgtc
     6721 gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg gtggttttag cgaactgcaa
     6781 agcgctattc gcattcatgg taacgcggtg gaatatattc ccatcgcgat tgtgctgatg
     6841 ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc atatttgcgg catcgttttg
     6901 cttgctggtc gtctgatgca ttattacggt tttcatcacc gtctgtttcg ctggcgacgt
     6961 tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg tgctggcgaa tctttggtat
     7021 atgccctggg agttggtttt ctccctgcgt tagcgcacaa tacgccactt tctttttccc
     7081 ggatttttac gttatgtctc accgcgacac gctattttct gcccctatcg ccagactggg
     7141 cgactggacc tttgatgaac gggtagctga agtcttcccg gatatgatcc agcgttccgt
     7201 tcccggctat tccaatatta tttccatgat tggtatgtta gccgagcgct tcgttcaacc
     7261 tggtacgcag gtttacgatc tgggttgttc tctgggcgcg gcgacgctct cggtgcgtcg
     7321 caacattcat catgataatt gcaaaattat tgccatcgac aactccccgg cgatgattga
     7381 acgctgcagt cgtcatattg acgcctataa agcccctacg ccagtagacg tcattgaagg
     7441 tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg gtgctgaatt ttaccctgca
     7501 attcctggaa ccttccgagc gccaggcatt actggataaa atttatcaag gactgaaccc
     7561 gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa gatgccaaag ttggtgaact
     7621 gttgttcaac atgcaccacg actttaaacg tgccaacggt tacagcgaac tggagatcag
     7681 ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat tccgtggaaa cccataaagc
     7741 acgcctgcat aaagccggtt ttgagcatag cgagctgtgg ttccagtgct ttaactttgg
     7801 ttcactggtg gcattaaaag cagaggacgc tgcatgatcg actttggtaa cttttattct
     7861 ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc tgcccgcgca gattgctaac
     7921 tggcagcgcg agcagcagca cgggctgttt aagcagtggt ccaacgcggt ggaatttctg
     7981 cctgaaatta aaccgtatcg tctggattta ttgcatagcg taaccgccga aagcgaagag
     8041 ccactgagca ccgggcaaat taaacgcatt gaaacgctta tgcggaacct gatgccatgg
     8101 cgcaaagggc cgttctcact gtatggcgtc aacatcgata ccgaatggcg ttccgactgg
     8161 aaatgggatc gtgttctgcc ccatctttct gatttaaccg gacgcaccat tctggatgtc
     8221 ggctgcggca gcggttatca catgtggcgc atgattggcg caggcgcgca cctcgcggtg
     8281 gggatcgacc ctacgcaact gtttctgtgc cagtttgaag cggtgcgtaa actactgggt
     8341 aacgatcagc gcgcacattt gttaccgtta ggtattgaac agcttccggc actgaaagcc
     8401 tttgataccg tcttttcgat gggcgtgctt taccatcgcc gttcgccgct ggagcatctc
     8461 tggcagttga aagatcaact ggtgaatgag ggtgaactgg tgctggaaac gctggttatt
     8521 gatggcgatg agaacacggt gctggtacca ggcgatcgtt acgcgcaaat gcgtaatgtc
     8581 tatttcatcc cttccgcgct ggcgctgaaa aactggctga agaagtgtgg ttttgtagat
     8641 atccgcattg tggatgtgtg cgttaccact actgaagagc agcgacgcac tgaatggatg
     8701 gttaccgaat ctctgtccga tttcctcgac ccacatgatc cgagtaagac agttgaaggt
     8761 tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc cgtaaaggtc tggtattact
     8821 gccggatgcg gcgtgaatgc cttatccggc ctacaaaccc ttgctaattc aatctattgc
     8881 agggactatg taggcctgat aagcatagcg catcaggcag ttttacgttt gcataacctc
     8941 agcgcccgtt tccgggcgct attcacttct tacgcctgaa cagcaggctt atcaaatgcc
     9001 gttaacttcg gtgcgttacc cgtatatttt tcgatataca ccagcgcaga gttaccggca
     9061 caggcatttg ccagctgcga gctggggata tccgccgtta acacgttcgc actgccgttt
     9121 ttacacaagc cgttttccag atctggccat gcaccttcat gcacgcatac cacgcctttt
     9181 ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac cacgtttgtt ccagacacgc
     9241 accagatcgc cattcgcaat actaaatcga gcagcatctt cggtgtgaat agtgatcggt
     9301 tcacgatcgg caaccgcata ttttttacgc agttccgcat agttaagctg actgtgtaaa
     9361 cggtgtgccg gatgtgcggt cagaagctgc aactgcttct cgtcggcggt acccttccac
     9421 tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct tatagccaaa tttttccagc
     9481 gttttggagt aaatctcaat tttgccactt ggcgtaccca gcgcattttt caccggatcg
     9541 gcgcggaaat caccataacg aacgtactgt tcgttcttct cgctgcggcg catttcgatc
     9601 agtttatttt gctgccagaa ggcattaaac attggcatag tgacgcgttg cgcacgggcc
     9661 cctttctgag cggcatcata gaaaaatttc agccacgcca tttcatcttt accttcggta
     9721 tagatctctt ttccgccagg tttgagtaat tccgcaagat cggcaaacac atcaaaatcg
     9781 tttcgcgctt caaattgcgg agcaacagcc tgcttcatcg gcacaatatg ctggttgctg
     9841 taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg tggtgatcgg taatacgata
     9901 tctgcgtgtt tagcagctgc ggtccagtag cattcagaaa cgacgatcat ctccggtttc
     9961 tgccatgctt taatcagacg attagtatcc tggtgatggg taaagttacc gccacccgcc
    10021 caccagatca ttttgatatt cggatacgtt tgctctttac cgttatgctg atatttgccg
    10081 ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa tcgccctcat tccgccatca
    10141 tcagcggcct cgctggcctg tccggcaatc gccgcagaca tttccggcag cacgccgcca
    10201 acacgtgtcg ggttgccacc attggagtaa tgataagaga aaccaaaacc gccgcccggt
    10261 gtaccaatct gccctaacat cgccgccagc gtcaccagca tccagtgttt ctgttcacca
    10321 tattgctggc gctgaattcc ccagcctgcc atcagcattg tgcgattcgc cgccatcagt
    10381 tcagcgagtt tgactatctg cgcttccggt acgccagtaa tttccgccgc ccaggccgcg
    10441 ctcttcggcg tattatcgct cttacctctc agatactctt caaactgcgg atacccggta
    10501 gtgtattttt caaggaacac tttatcgtgt ttgccttgtg tcatcagggt atgcgcaatc
    10561 cctaacatca gtgccacgtc ggtgcccata ttcggcgcga tccaggtggc attatcgcca
    10621 aagaagtcga tggtttcgga gcggatagga tcaatggcaa tcactggttt gccagatttt
    10681 ttcagctgat ggaagtattc cagcccttgc tcatcggtac tgctccaggc aatttttaag
    10741 gtattcagcg ggttcatccc ccacagcacc acaacctggc tgttttccag aatcagcggc
    10801 caggaggtct gctgttcata cacctctaca gaaccgacca catgcggcat gatcacctgt
    10861 gccgccccgg ttgaataatc gccgctatgc ccggaataac cgcccgccag gttcatataa
    10921 cgttgcagta aggtttgcgc tttatgcaac acgccagaag agcgccagcc gtatgatccg
    10981 gcaaaaatag ccgatggtcc gttagctttg cgaatgcgat cgtgttgctc atgaatcagt
    11041 tttaatgctt gttcccagct cacctgtaca taggtatctt cgccacgacc tttcgtcggt
    11101 tgcagtggat tatcgagata gctttttctc accatcggat gctgaatacg cgtcgtggtg
    11161 tgtacctgat ccgccgccgt agactgtaag gaattcggta tggttttcgc cagcgcgcct
    11221 gttgaagaaa caatcttgcc gtccttcact tctacgttca tcgctcccca acgttccgcg
    11281 gtgaggattt taccgtc
//
LOCUS       DAZPJR010000136        11243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPJR010000136 DAZPJR010000000
VERSION     DAZPJR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.137"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            3..308
                     /locus_tag="ACHVB7_003276"
     CDS             3..308
                     /locus_tag="ACHVB7_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR1915180.1"
                     /translation="MLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="ACHVB7_003277"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="ACHVB7_003277"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR1915181.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHVB7_003278"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHVB7_003278"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR1915182.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHVB7_003279"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHVB7_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR1915183.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHVB7_003280"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHVB7_003280"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR1915184.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHVB7_003281"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHVB7_003281"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR1915185.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHVB7_003282"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHVB7_003282"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR1915186.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHVB7_003283"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHVB7_003283"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR1915187.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHVB7_003284"
     CDS             complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHVB7_003284"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR1915188.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHVB7_003285"
     CDS             complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHVB7_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR1915189.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHVB7_003286"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHVB7_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR1915190.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..11243
                     /locus_tag="ACHVB7_003287"
                     /pseudo
     CDS             10353..11243
                     /locus_tag="ACHVB7_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2554 a         3090 c         2950 g         2649 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtga aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cgt
//
LOCUS       DAZPJR010000137        11217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPJR010000137 DAZPJR010000000
VERSION     DAZPJR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.138"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHVB7_003288"
     CDS             53..352
                     /locus_tag="ACHVB7_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR1915191.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHVB7_003289"
     CDS             complement(472..651)
                     /locus_tag="ACHVB7_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR1915192.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHVB7_003290"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHVB7_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR1915193.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHVB7_003291"
     CDS             2090..2668
                     /locus_tag="ACHVB7_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR1915194.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHVB7_003292"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHVB7_003292"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1915195.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHVB7_003293"
     CDS             4347..5945
                     /locus_tag="ACHVB7_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1915196.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHVB7_003294"
     CDS             complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHVB7_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR1915197.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHVB7_003295"
     CDS             complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHVB7_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR1915198.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7962..8933
                     /gene="manX"
                     /locus_tag="ACHVB7_003296"
     CDS             7962..8933
                     /gene="manX"
                     /locus_tag="ACHVB7_003296"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR1915199.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8996..9796
                     /gene="manY"
                     /locus_tag="ACHVB7_003297"
     CDS             8996..9796
                     /gene="manY"
                     /locus_tag="ACHVB7_003297"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR1915200.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9809..10660
                     /gene="manZ"
                     /locus_tag="ACHVB7_003298"
     CDS             9809..10660
                     /gene="manZ"
                     /locus_tag="ACHVB7_003298"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR1915201.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10715..11173
                     /locus_tag="ACHVB7_003299"
     CDS             10715..11173
                     /locus_tag="ACHVB7_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR1915202.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2757 a         2822 c         2829 g         2809 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca gctcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggg ttatcggcat cgtgctgaac attggcctga
     6721 cgtttcccgc ccatcaaacg caggatggca tcggcagtac gcgctcgcag cggcaaagtc
     6781 gactggtggc gaataaagtt gcgacgcgca atctggttaa acacttcgat gatgatcgag
     6841 aagccaatcg cggcatacag gtaacctttc ggaatgtgga aaccgaaacc ttctgccacc
     6901 agactcagac caatcattaa caggaagctc agacagagca ccaccaccgt ggggtgctgg
     6961 ttaacgaatc gcgtcagcgg tttggatgcc agcaacataa ccgccatcgc aatcactacc
     7021 gccgccatca tcaccggcag atggttaacc atccctactg cagtaattac cgcatccaac
     7081 gagaagacgg cgtcaaggat gacaatctgt gtgacgacca cccagaaact ggcgtagcct
     7141 ttaccgtggc cggaatcatg atcgcggttt tccagccgtt catgcagttc ggttgttgct
     7201 ttgaacagca agaatatccc cccgaacaac ataatcaggt cgcgtccgga gaaggagaaa
     7261 tccatgacgg taaatagcgg tttggtcagc gtgaccatcc atgaaatcag cgacagcagc
     7321 cccagacgca taatcagcgc cagtgataac cccagcaaac gcgctttatc gcgttgtttt
     7381 ggcggcagtt tgtcagcaag aatggcgatg aagaccaggt tatcgatacc cagcacaatt
     7441 tcgagaacaa caagcgtgag tagccccgcc caaattgagg ggtccattaa gaattccatg
     7501 acaagctcct gcttaaggaa tagctattcg acgccagaaa taatgcaggc gtaacgacaa
     7561 aatgcaaacg aaaggtgcgg catagagtgc cagaaaggca ggcgttaaaa ggcctgatgc
     7621 tgaaatgacg tcggtgacga tccatactgc gggctactgc cctatactcc atggttgtta
     7681 aacgggagtt aaacatatca gagacgcctc tgatttggca aagatttacc ttcctttgca
     7741 aacgaatgtg acaaggatat tttacctttc gaaatttctg ctaatcgaaa gttaaattac
     7801 ggatcttcat cacataaaat aattttttcg atatctaaaa taaatcgcga aacgcagggg
     7861 tttttggttg tagcccttat ctgaatcgat tcgattgtgg acgacgattc aaaaatacat
     7921 ctggcacgtt gaggtgttaa cgataataaa ggaggtagca agtgaccatt gctattgtta
     7981 taggcacaca tggttgggct gcagagcagt tgcttaaaac ggcagaaatg ctgttaggcg
     8041 agcaggaaaa cgtcggctgg atcgatttcg ttccaggtga aaatgccgaa acgctgattg
     8101 aaaagtacaa cgctcagttg gcaaaactcg acaccactaa aggcgtgctg tttctcgttg
     8161 atacatgggg aggcagcccg ttcaatgctg ccagccgcat tgtcgtcgac aaagagcatt
     8221 atgaagtcat tgcaggcgtt aacattccaa tgctcgtgga aacgttaatg gctcgtgatg
     8281 atgacccaag ctttgatgaa ctggttgcgc tggcagtaga aacaggccgt gaaggcgtga
     8341 aagcactgaa agccaaaccg gttgaaaaag ccgcgccagc acccgctgcc gcagcaccaa
     8401 aagcggctcc aactccggca aaaccaatgg ggccaaacga ctacatggtt attggccttg
     8461 cgcgtatcga cgaccgtctg attcacggtc aggtcgccac ccgctggacc aaagaaacca
     8521 atgtctcccg tattattgtt gttagtgatg aagtggctgc ggataccgtt cgtaagacac
     8581 tgctcaccca ggttgcacct ccgggcgtaa cagcacacgt agttgatgtt gccaaaatga
     8641 ttcgcgtcta caacaacccg aaatatgctg gcgaacgcgt aatgctgtta tttaccaacc
     8701 caacagatgt agagcgtctc gttgaaggcg gcgtgaaaat cacctctgtt aacgtcggtg
     8761 gtatggcatt ccgtcagggt aaaacccagg tgaataacgc ggtttcggtt gatgaaaaag
     8821 atatcgaggc gttcaagaaa ctgaatgcgc gcggtattga gctggaagtc cgtaaggttt
     8881 ccaccgatcc gaaactgaaa atgatggatc tgatcagcaa aatcgataag taacgtattg
     8941 tgttgattat cactcagttt tcacacttaa gtcttacgta aacaggagaa gtacaatgga
     9001 gattaccact cttcaaattg tgctggtatt tatcgtagcc tgtatcgcag gtatgggatc
     9061 aatcctcgat gaatttcagt ttcaccgtcc gctaatcgcg tgtaccctgg tgggtatcgt
     9121 tcttggggat atgaaaaccg gtattattat cggtggtacg ctggaaatga tcgcgctggg
     9181 ctggatgaac atcggtgctg cagttgcgcc tgacgccgct ctggcttcta tcatttctac
     9241 cattctggtt atcgcaggtc atcagagcat tggtgcaggt atcgcactgg caatccctct
     9301 ggccgctgcg ggccaggtac tgaccatcat cgttcgtact attaccgttg ctttccagca
     9361 cgctgcggat aaggctgctg ataacggcaa cctgacagcg atttcctgga tccacgtttc
     9421 ttctctgttc ctgcaagcaa tgcgtgtggc tattccggcc gtcatcgttg cgctgtctgt
     9481 tggtaccagc gaagtacaga acatgctgaa tgcgattccg gaagtggtga ccaatggtct
     9541 gaatatcgcc ggtggcatga tcgtggtggt tggttatgcg atggttatca acatgatgcg
     9601 tgctggctac ctgatgccgt tcttctacct cggcttcgta accgcagcat tcaccaactt
     9661 taacctggtt gctctgggtg tgattggtac tgttatggca gtgctctaca tccaacttag
     9721 cccgaaatac aaccgcgtag ccggtgcgcc tgctcaggca gctggtaaca acgatctcga
     9781 taacgaactg gactaacagg tgagcgaaat ggttgataca actcaaacta ccaccgagaa
     9841 aaaactcact caaagtgata ttcgtggcgt cttcctgcgt tctaacctct tccagggttc
     9901 atggaacttc gaacgtatgc aggcactggg tttctgcttc tctatggtac cggcaattcg
     9961 tcgcctctac cctgagaaca acgaagctcg taaacaagct attcgccgtc acctggagtt
    10021 ctttaacacc cagccgttcg tggctgcgcc gattctcggc gtaaccctgg cgctggaaga
    10081 acagcgtgct aatggcgcag agatcgacga cggtgctatc aacggtatca aagtcggttt
    10141 gatggggccg ctggctggtg taggcgaccc gatcttctgg ggaaccgtac gtccggtatt
    10201 tgcagcactg ggtgccggta tcgcgatgag cggcagcctg ttaggtccgc tgctgttctt
    10261 catcctgttt aacctggtgc gtctggcaac ccgttactac ggcgtagcgt atggttactc
    10321 caaaggtatc gatatcgtta aagatatggg tggtggcttc ctgcaaaaac tgacggaagg
    10381 ggcgtctatc ctcggcctgt ttgtcatggg ggcattggtt aacaagtgga cacatgtcaa
    10441 catcccgctg gttgtctctc gcattactga ccagacgggc aaagaacacg ttactactgt
    10501 ccagactatt ctggaccagt taatgccagg cctggtacca ctgctgctga cctttgcttg
    10561 tatgtggcta ctgcgcaaaa aagttaaccc gctgtggatc atcgttggct tcttcgtcat
    10621 cggtatcgct ggttacgctt gcggcctgct gggactgtaa gactgttgta cactaccggg
    10681 gccttttggc cccgtttttt tatctggagg attaatgaca atcacggacc tggtactgat
    10741 tcttttcatc gccgcactcc tggccttcgc gatctacgat cagttcatca tgccccgccg
    10801 taacggcccc accctgctgg caattccttt gctccggcgt ggtcgcatcg atagcgttat
    10861 cttcgtcgga ttgattgtca ttcttatcta taacaacgtc acgaatcatg gtgcgttaat
    10921 aacgacatgg ttattaagcg cactggctct gatgggtttt tatatattct ggatccgcgt
    10981 tccgaagatc atctttaaac aaaaaggttt tttcttcgcc aatgtctgga ttgaatatag
    11041 ccgaatcaaa gcgatgaact tgtcggaaga tggcgtgttg gtgatgcaat tagaacagcg
    11101 tcggctgtta atccgcgttc gaaatatcga cgatctggaa aaaatttata agcttctcgt
    11161 ttcaactcaa taagttatga atttagccaa agctatgttt agtgtatttt taataat
//
LOCUS       DAZPJR010000138        11212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPJR010000138 DAZPJR010000000
VERSION     DAZPJR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.139"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1285
                     /gene="narU"
                     /locus_tag="ACHVB7_003300"
     CDS             <1..1285
                     /gene="narU"
                     /locus_tag="ACHVB7_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR1915203.1"
                     /translation="KTITYLSDTGCLEIQGASLAVTVNLNKIGFNFTTDQLFLLTALP
                     SVSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALL
                     CGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFL
                     GVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLH
                     LWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISD
                     KFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQM
                     IAVIFRQITIYRVKMKGGSDEQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLN
                     MTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1367..5107
                     /gene="narZ"
                     /locus_tag="ACHVB7_003301"
     CDS             1367..5107
                     /gene="narZ"
                     /locus_tag="ACHVB7_003301"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR1915204.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5104..6648
                     /gene="narH"
                     /locus_tag="ACHVB7_003302"
     CDS             5104..6648
                     /gene="narH"
                     /locus_tag="ACHVB7_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1915205.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6648..7343
                     /gene="narW"
                     /locus_tag="ACHVB7_003303"
     CDS             6648..7343
                     /gene="narW"
                     /locus_tag="ACHVB7_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1915206.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7340..8020
                     /gene="narI"
                     /locus_tag="ACHVB7_003304"
     CDS             7340..8020
                     /gene="narI"
                     /locus_tag="ACHVB7_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1915207.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8099..8992
                     /locus_tag="ACHVB7_003305"
     CDS             8099..8992
                     /locus_tag="ACHVB7_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR1915208.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9088..9933)
                     /gene="nhoA"
                     /locus_tag="ACHVB7_003306"
     CDS             complement(9088..9933)
                     /gene="nhoA"
                     /locus_tag="ACHVB7_003306"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR1915209.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10106..10675
                     /locus_tag="ACHVB7_003307"
     CDS             10106..10675
                     /locus_tag="ACHVB7_003307"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR1915210.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10679..10906)
                     /gene="pptA"
                     /locus_tag="ACHVB7_003308"
     CDS             complement(10679..10906)
                     /gene="pptA"
                     /locus_tag="ACHVB7_003308"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR1915211.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11006..>11212)
                     /locus_tag="ACHVB7_003309"
     CDS             complement(11006..>11212)
                     /locus_tag="ACHVB7_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1915212.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2608 a         2922 c         2993 g         2689 t
ORIGIN      
        1 taagactatc acttatttaa gtgatactgg ttgtctggag attcaggggg ccagtctagc
       61 agttaccgtt aatctcaata aaatcggttt taattttact accgatcaac tctttttatt
      121 aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc
      181 tatattcggt ggacgccgat ggacggtttt tagtactgca atcctgatta ttccttgcgt
      241 ctggctcgga attgccgtgc aaaatccgaa tactcctttt gggatattta tcgttatcgc
      301 tttgctatgc ggttttgcag gtgcaaactt tgcttcgagc atgggcaata tcagtttctt
      361 ctttccaaaa gccaaacaag ggagcgctct tgggattaat ggcggattag gaaacttagg
      421 tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt gtacctgtat ttgcctttct
      481 cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga atgccgcatg
      541 gatttgggta ccgctactgg cgattgccac gatcgccgcc tggtcaggga tgaatgatat
      601 cgccagttca cgcgcctcaa ttgacgacca gctccctgtc ttacaacgcc tgcatctctg
      661 gctgctgagc ctgctttacc ttgccacctt cggttcgttt atcggttttt ctgcgggttt
      721 tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg
      781 cccatttatc ggtgccatcg cgcggtcggt tggtggtgct atttccgata aattcggcgg
      841 cgtgcgggtg acgttgatca actttatttt tatggcgatt ttcagtgccc tgctgttcct
      901 taccttaccg ggcacaggct ccggtaattt catcgccttt tacgccgtat ttatggggct
      961 gtttctgact gcgggtctgg gaagtggttc tactttccag atgatcgccg tcatctttcg
     1021 ccagataacc atttatcggg tgaagatgaa aggcggtagt gatgagcaag ctcagaaaga
     1081 agccatcacc gaaacggcgg cggctctggg ctttatctca gccattggcg cagtgggcgg
     1141 cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg accggctctc cggtcggcgc
     1201 gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg
     1261 tcggcggaag ttcagccaaa aataagagat cgatgcctga tgtacaaccg tgtcgcatcc
     1321 gacaaggcat cagatcccaa ttcattcctg gagcaggagt catgtcatga gtaaactttt
     1381 ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt gccgatggtc acggacaggt
     1441 gatgcatagc aaccgcgact gggaggacag ctatcgccag cgttggcagt tcgacaaaat
     1501 tgtgcgttcc acccacggtg ttaactgtac aggctcctgt agctggaaaa tctacgttaa
     1561 aaatggtctg gtgacctggg aaatccaaca gaccgactac ccgcgcactc gccctgacca
     1621 gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt atctctacag
     1681 cgctaaccgc ctgaaatacc cgctcattcg taaacgactg attgaactgt ggcgtgaagc
     1741 tctcaagcaa cacagcgatc cggtactggc gtgggcatcg attatgaacg atccgcaaaa
     1801 gtgcttaagc tacaaacaag tgcgtgggcg cggcgggttt atccgctcca actggcagga
     1861 acttaaccaa ctgattgccg ccgctaacgt ctggaccatc aaaatctatg gtccggatcg
     1921 cgttgccggt ttctcgccga tcccggcgat gtcgatggtg tcatacgctg ccggaacgcg
     1981 ttatctgtcg ctgttaggcg gcacctgttt aagcttctac gactggtatt gcgacctgcc
     2041 gcccgcctcg ccgatgacct ggggcgaaca aaccgacgtg ccggaatctg ccgactggta
     2101 taactccagt tacatcatcg cctgggggtc taacgtaccg cagacacgta cgccggacgc
     2161 ccacttcttt accgaagtac gttacaaagg caccaaaact atcgccatta cccctgacta
     2221 ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg aaacaaggca ctgatagcgc
     2281 cctggcgatg gcaatgggcc atgtgatttt aaaagagttt catctcgata atcccagcga
     2341 ctactttatc aactactgcc gccgctatag cgacatgccg atgctggtaa tgctggaacc
     2401 tcgcgacgat ggtagctacg tacccgggcg catgatccgc gcgtctgacc tggtagatgg
     2461 actaggcgaa agcaataatc cgcagtggaa aaccgtggcg gttaataccg caggtgaatt
     2521 ggtagtgccg aatggttcga ttggtttccg ctggggagaa aaaggcaaat ggaatctgga
     2581 atccattgcc gccggtaagg aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc
     2641 tgttgcaggc gtggccttcc cctactttgg cggcattgaa aatccgcatt ttcgcagcgt
     2701 aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg
     2761 caacacctgt ccggtggtca gcgtttatga tttggtgctg gcgaattacg gcctcgatcg
     2821 cgggctggaa gatgaaaaca gcgcgaaaga ttacgctgaa atcaaaccgt acaccccagc
     2881 ctggggtgag caaattaccg gcgtaccgcg ccagtatatt gaaaccatcg cccgcgaatt
     2941 tgccgatacc gcccataaaa cgcatggacg ctcaatgatt atcctcggcg ctggcgtcaa
     3001 ccactggtat cacatggata tgaactaccg tgggatgatc aatatgctga tcttctgcgg
     3061 ttgtgtcggg caaagcggcg gcggctgggc gcactatgtc ggtcaggaaa aactgcgccc
     3121 acaaaccggc tggttgccgc tggcctttgc gctcgactgg aaccgcccac cgcgccagat
     3181 gaacagtacc tcgtttttct acaatcattc cagccaatgg cgctatgaaa aagtctctgc
     3241 tcaggagtta ctttcaccgc ttgccgatgc cagtaagtac agcggtcatc tgattgattt
     3301 caacgttcgc gccgaacgca tgggctggct accttctgcg ccgcagctgg ggcgtaaccc
     3361 gctcgggatt aaagctgaag ccgacaaggc cggattatcc cccacagaat ttaccgccca
     3421 ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa ccagatagcg gcagcaatca
     3481 tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc tcctccggca aaggccacga
     3541 gtatatgcag aagtatctgc tggggaccga aagcgggatt cagggcgagg aactcggtgc
     3601 cagcgacggg atcaaaccgg aagaagtcga gtggcaaact gcggcgattg aaggcaagct
     3661 cgacctgctg gtgacgctcg acttccgcat gtccagtacc tgcctgttct ccgatatcgt
     3721 tctgcccacc gccacctggt acgaaaaaga cgatatgaac acctcggata tgcatccgtt
     3781 tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat
     3841 ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac
     3901 cgacgtggta ttacaacccc tactgcatga ctctccggca gagctctcac agccgtgtga
     3961 agtgctcgac tggcgcaaag gcgaatgcga tctgattcca ggcaaaaccg cgccgaatat
     4021 tgtggcggtg gagcgcgact accctgctac gtatgaacgc tttacctcgc tcgggccatt
     4081 gatggacaaa cttggcaatg gtggtaaagg gatttcgtgg aatacccagg atgaaatcga
     4141 tttcctcggc aaactcaatt acaccaagcg tgatggcccg gcacaggggc gtccgctgat
     4201 tgacaccgcc attgacgctt cagaagtgat tctggcactg gcaccggaaa ccaacggtca
     4261 tgttgcagtc aaagcgtggc aggcgctggg cgagatcacc gggcgcgaac atacccatct
     4321 ggcgctgcac aaagaggacg agaagattcg ctttcgcgat attcaggcgc agccgcgtaa
     4381 aattatctcc agccccacat ggtccggtct ggaaagcgat cacgtctcct ataatgcggg
     4441 atacaccaac gttcatgagt taattccgtg gcgcacgctg tcgggacgcc agcagctcta
     4501 tcaggatcat ccgtggatgc gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat
     4561 cgacacccgt agcgtcagtg agatgcgcca gatcccgcca aacggcttcc cggaaaaagc
     4621 acttaacttc ctgacgccgc accagaaatg gggcattcac tcaacctaca gtgaaaacct
     4681 gctaatgctg acgctctctc gcggtggacc gattgtctgg atcagcgaaa ccgatgcccg
     4741 agaactgacc attgtcgata acgactgggt ggaagtcttt aacgccaacg gcgcgctgac
     4801 ggctcgtgcg gtggtcagcc aacgtgtacc gccgggtatg accatgatgt atcacgccca
     4861 ggaacgcatt atgaatattc ctggttcgga agtaactggc atgcgcggcg gcattcataa
     4921 ctcggtcacc cgtgtttgcc cgaaaccgac gcatatgatt ggcggttacg cgcagctggc
     4981 ctggggcttt aactactacg gcaccgtcgg ctcgaaccgc gacgaattca tcatgatccg
     5041 caagatgaag aacgttaact ggctggatga tgaaggtcgc gatcaggtac aggaggcgaa
     5101 aaaatgaaaa tacgttcgca agtcggcatg gtgcttaacc tcgacaaatg tatcggttgc
     5161 catacctgtt cggtgacctg taaaaacgtc tggaccggac gcgaaggcat ggagtacgca
     5221 tggtttaaca acgtcgaaac caaaccgggc attggttatc cgaaaaactg ggaagatcag
     5281 gaagagtggc aaggcggctg ggtccgcgat gtgaatggca agatacgccc gcgtctgggc
     5341 agcaagatgg gcgtaataac caaaatcttc gccaacccgg tggtgccgca gattgatgat
     5401 tactacgaac ctttcacctt cgactacgaa catttgcata gcgcaccgga aggcaaacat
     5461 atccctactg ctcgcccgcg ttcgctgatt gacggtaagc ggatggacaa agtgatctgg
     5521 gggccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5581 ttcgaggcca tgcaaaagga gatgtacggg cagtttaaaa acaccttcat gatgtacctg
     5641 ccgcgcctgt gcgaacactg cctcaatccc agttgcgtgg cgacctgccc aagcggcgct
     5701 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     5761 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     5821 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     5881 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatt
     5941 gaggaagcgg cgagcaccga gcgcgaagtt gacctctatg aacgtcagtg cgaagtgttc
     6001 ctcgatccac acgatccctc agtgatcgag gaagccctga aacaaggtat tccacaaaac
     6061 gtgattgaag ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg
     6121 ctaccgctgc accctgaata tcgcaccctg ccaatggtct ggtacgttcc tccgctgtcg
     6181 ccgattcagt cctacgcaga tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc
     6241 atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc
     6301 ggtccggtac tgcgggcgct gaaacggatg atggcgatgc gccactatat gcgttcacaa
     6361 accgtggaag gcgttactga tactcgtgcc atcgacgaag taggcctgag cgtcgcccag
     6421 gtcgaagaaa tgtatcgcta tctcgccatt gccaactatg aagatcgttt tgttatcccg
     6481 accagccatc gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg cggttttacc
     6541 tttggcgacg gttgccacgg ttcggacagt aaattcaacc tgttcaacag tagccgtatc
     6601 gatgccatca acatcaccga agtgcgcgac aaagcggagg gcgaataatg cagatcctca
     6661 aagtgatcgg cctgttgatg gagtatccgg atgagctgtt gtgggaatgc aaggaggacg
     6721 cgctggcgtt gatccgccgc gacgcgccga tgcttacgga tttcacccac aacctgctta
     6781 acgcgccgct gctggataaa caggccgaat ggtgcgaagt gtttgaccgc gggcgcacca
     6841 cgtcgctgct gctgttcgaa catgttcatg ccgagtcccg cgatcgcggc caggcaatgg
     6901 tggacctgct ggcggagtat gaaaaggtcg gcctgcagct ggattgtcgg gaactgcccg
     6961 attatctacc gctgtatctg gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag
     7021 gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg
     7081 caccgtggta cgcgttgttt gatgctctgc tgcaactggc gggaagtata ctttcaagtg
     7141 acagtgtcac aaaacaagta aacagcgaag agcgcgatga cacccgccag gcgcttgatg
     7201 cagtgtggga agaggaacag gtgaagttta ttgaagataa cgccacggcg tgtgacagct
     7261 cgccgcttaa tcaatatcag cgacgcttta gccaggatgt cgcgccgcag tatgtcgaca
     7321 tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc tttttttacg atatctaccc
     7381 gtacatttgt gcgacggtgt ttttcctcgg cagctggctg cgctacgact acgggcagta
     7441 cacctggcgc gcctcctcaa gtcaaatgct cgataaacgc gggatggtga tatggtcgaa
     7501 tctgttccat atcggcattt tggggatttt cttcgggcac ttgttcggca tgttaacgcc
     7561 gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa caactcatgg cgatggtgct
     7621 cggcggtatt tgcggtgtgt tgacgttgat tggcggcgca ggactgctgt ggcgcagtct
     7681 gacaaatcag cgcgtgcgtg ccacttccac tacgccggat atcatcatca tgagcatttt
     7741 gctgatccag tgtctgttgg ggctaagtac cataccgttt tcagcacagt atcctgatgg
     7801 tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt gtcactttcc gtggtggatc
     7861 gtcagagatg ctcaacggcg tagcgttcgt cttccgcctg catctggtgt tgggaatgac
     7921 catcttcctg ctcttcccgt tcacccgact ggtgcatgtg tggagcgcgc cgtttgagta
     7981 ctttactcgt cgatatcaaa ttgtgcgttc gcggcgataa tttcaccctg gccggatagc
     8041 ccttcgctct ccggccactt gcaggctatg atagttctcc agtcacagga gaacgttaat
     8101 gaaaccgcag gtgtaccacg tcgatgcttt tacctctcaa ccatttcgcg gcaattctgc
     8161 cggtgttgtt ttccccgccg ataatcttag cgaagcacaa atgcagctta tcgcccgcga
     8221 gttaggccat tcggaaactg cttttctgct gcacagcgac gacagcgatg tgcgcatccg
     8281 ctactttacg ccaacggttg aagtgccgat ttgcggtcac gcgacagtag ctgcgcacta
     8341 tgtacgtgcg aaggtgttag gtttaggaaa ttgcacgatc tggcaaacat cgctggcagg
     8401 aaaacatcgc gtgactatcg aaaagcacaa cgatgattat cgtatttcgc tggaacaagg
     8461 tacgccgggc tttgaactgc cgctggaggg tgaagtacgc gcggcgatta tcaacgcatt
     8521 gcatcttact gaagacgata ttctgccggg tttgccgatc caggtcgcga cgaccgggca
     8581 ttctaaagtg atgatcccat tgaagccgga agtggatatc gacgctcttt cgcctgacct
     8641 taatgcgctg accgctatca gtaaacagat tggctgcaat ggtttcttcc cgttccagat
     8701 ccgtccaggc aaaaacgaaa ccgatggtcg tatgttctcg cctgcgattg gtattgtgga
     8761 agatccggtg actggcaatg ccaatggccc gatgggtgca tggttggtac atcacaacgt
     8821 attgccccac gatggcaacg tgttgtgtgt taaaggccat caggggcgcg cattggggcg
     8881 tgacggcatg attgaagtga cggtgacaat tcgtgataac caaccggaaa aagtcaccat
     8941 ttcaggcaca gcggtgattt tgtttcatgc cgaatgggca attgaaatct gaaatcgttg
     9001 cctgatgcgc ttcgcctgtc aggtctacaa ccgggctaat gtatgccgga taaggcgttt
     9061 acgccgcatc cggcaacctg acataaatta ttttcccgcc tccggatgcg tatcaaacgc
     9121 cgccatcacc agcgctaact catccacagt aaagccatgt ttcgcatcat ccacgcccag
     9181 accaaactgt tcctgcatca cggcatataa cgacgccaca tccggtagat ttcgctgctc
     9241 caccgcgtgc ccattttcat aatgggtaaa atgaaaattg gtcagtgtca gcttgccgcc
     9301 gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc
     9361 cgaccagaaa ttgcccatca cataatcgct ttgttgctgc tcgcagagat cgaagcggta
     9421 catcggctgc caatgctgat gatgattaaa ctgcaacacc caatcatcac cctcctgcaa
     9481 caaccgatac tctccgtgtg gcgtggtctg cacgagatcg gaaactaaac gaatcggcgc
     9541 ggttagcgtc tgcccaccga aaccgacatc agcaatccat ttttcccctt ccagttccac
     9601 caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga tttgataaca ctacgcgccc
     9661 taacaagctg cgaacgttaa accccagctc gcgtaacacc cgctcaaaca cgccattctg
     9721 ctcaaaacag taaccgccac gacgggctat caccagtttc tcttccagcg attgatcatc
     9781 aagctgtatt tccctcggca gcaaaacgtc aaggttttca aacggaatgg tgcaattgtg
     9841 tttcaggtgc aatgcacgaa gcgtatcaat attgaccgca gcagctcccg accagttaat
     9901 acgggcaaaa tagtgattca gaatgggcgt catcaggcgt ttcccttagt ggttttctca
     9961 acttataacc tggaatagac gccgcaccgg ctattttgtg cgatcgcgga catgcatcac
    10021 gtttgcaaat cgccaatccc gttaatttcg ttacttattg cgttacggac tacgcttaaa
    10081 aaaaccttct tcgcgaggcg ataccatgag ccgattcatc cccatagaat tacaccatgc
    10141 cagccgtctg ttaaatcatg gcccaactgt cttgatcacc agtttcgatg agcaatccca
    10201 gcggcgtaac attatggctg cagcctggtc aatgccggtg gagtttgaac ctccacgcgt
    10261 ggcaattgtg gtagataaat cgacatggac cagggagttg attgaacgta acggtaagtt
    10321 tggcatcgtt atcccgggcg ttgcagcaac taactggacg tgggcggtgg gaagtgtgtc
    10381 ggtgcgtgaa gaagataaat ttaattgcta tggcattccg gtggtgaaag gtccggtgct
    10441 tggtttgcca cttgttgaag agaaatgcct ggcgtggatg gagtgccgcc tgctacctgc
    10501 gacttctgcg caacaaaaat acgacacgct gtttggcgag gtggtatcag cagcggcaga
    10561 cgcacgggta tttgtcgaag gccgctggca gtttgatgat gataagctca atacgttgca
    10621 tcatttaggt gctggcacgt ttgttactag cggcaagcgt gttacggcag gttaagcatt
    10681 acatgctgta accgggtttc tttatcaaag cctccatttg gggctcgatt tcggcatccc
    10741 agatagcttg ccaagattct ggttgaatct gctgtagagc aatgcttatc gagctatctt
    10801 tactgttcag atggcgaata ataacgtcgg taatatctgc agcaagtgct gctttttgtt
    10861 gttcgtccag ttcacgcgga aagcatttaa tgtcgatgtg cggcatgatt acgctccttt
    10921 tgaaagatgt cagtaaaatt aacacacatt gtgaatgtca tgtgtccggt aaagcgttta
    10981 tacgacaata atgcccaggg caagattacg aaagcccgct ccccgtaagg actgacgcca
    11041 ggtagtttct gcccatggct gcttttcgca tcttacgtct taaccctgcc ttgaatacct
    11101 tatcattcgt caaaatatta atagcaatgt gccgtatccc tgaaaataat tctgctgcat
    11161 ttcctcttct tattttgcag tcgtcttcat tcattaccac gtccagacgc ca
//
LOCUS       DAZPJR010000139        11139 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPJR010000139 DAZPJR010000000
VERSION     DAZPJR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.140"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            57..983
                     /locus_tag="ACHVB7_003310"
     CDS             57..983
                     /locus_tag="ACHVB7_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR1915213.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(933..2126)
                     /locus_tag="ACHVB7_003311"
     CDS             complement(933..2126)
                     /locus_tag="ACHVB7_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1915214.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2262..3986
                     /gene="iucA"
                     /locus_tag="ACHVB7_003312"
     CDS             2262..3986
                     /gene="iucA"
                     /locus_tag="ACHVB7_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR1915215.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3987..4934
                     /gene="iucB"
                     /locus_tag="ACHVB7_003313"
     CDS             3987..4934
                     /gene="iucB"
                     /locus_tag="ACHVB7_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR1915216.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4934..6676
                     /gene="iucC"
                     /locus_tag="ACHVB7_003314"
     CDS             4934..6676
                     /gene="iucC"
                     /locus_tag="ACHVB7_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR1915217.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6673..8010
                     /gene="iucD"
                     /locus_tag="ACHVB7_003315"
     CDS             6673..8010
                     /gene="iucD"
                     /locus_tag="ACHVB7_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR1915218.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8016..10211
                     /gene="iutA"
                     /locus_tag="ACHVB7_003316"
     CDS             8016..10211
                     /gene="iutA"
                     /locus_tag="ACHVB7_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR1915219.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDFLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10762..10905)
                     /locus_tag="ACHVB7_003317"
     CDS             complement(10762..10905)
                     /locus_tag="ACHVB7_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915220.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2799 a         2787 c         2882 g         2671 t
ORIGIN      
        1 cacaagttga aataatagta ttatattgaa aagacagaga ttatttcaga ggtgttatgg
       61 aaaactgggt tattcaggag ctaaaatcac tggatatagg ggactcaaga cttgataaaa
      121 gagtaaaaca tatcctcaat agcctcagtc gttcaccaga agaaagtatt cctgtttcct
      181 gccgtacctg gagtgaaaca aaggcagctt atcgttgttt ttcaaacgat aaagtctctg
      241 ctaataaaat tatggctcct cacaaagaaa atatcactga aagagctcag cagtttaaaa
      301 gagttttagt tttacaggat acaacagaac ttaattattc tggtcagaaa gaaaaacaag
      361 gagttggtca caaaaaacat aaagatgaaa gaaacttatt tcttcatcct caactcgtca
      421 tcagtgagtc aggtgtttgc ctcggtgttt atgatgacta tcagtggttc agggatgaat
      481 taaaaaccag taaaaatacc cgacaagaaa tctgcaatga tttacttcat aaaaaacatg
      541 tcagtgaaaa agaaacctgg cgttgggttg aaggatataa taaagcaaca gaactggctc
      601 gccaatgccc tgatacccat gtattgtcaa tttctgatcg tgaaggcgat ttttatgacc
      661 tgtttgaacg cgcagagcag actcccggaa taaaagctga ctggcttgtc agaatgaaat
      721 ttaataacag agcaacgctg aatattaatg gtaaacggga tcataggttg ttgcacgaaa
      781 gaatcatgga gattacaccg cagcaactgg ttgaattcac tatacctgat gggcgggggc
      841 agcaagccag aacactgcct gacatgcttt catcagacgc aggtcctctc tcatatcctc
      901 tgatttactc cagtaaggtg atgatcatat ctttatttga tagtacggta atgccagata
      961 aatacagaca caatggtaaa taatatagtt gcaaataatg ctgccataat cagcgcgatt
     1021 gcgacacctg ttttcgatac cagcggcacc agcaaagcgc acagtccata gccaagtgtg
     1081 tgactggtag cgagccagcc cgcgcctgcg ccatcagcca gcttatcatt cagtagaagt
     1141 tgataagcgg gtgttgccag agctgctcca aatgacagca ctgcacagcc cgggtaaaac
     1201 aaccatagct gttcggagag catgatagcc agaccaccac tcatcaacac tccggcactc
     1261 aggagcaggg ccaccggagt caggcgctgc ggacggagta ccccgaactg cgcgataagc
     1321 gcagctactg cggacagacc caacagccag gccacctgtt ggctaatggc ggtggtatcc
     1381 gtgacgaact ggcgagtaag ggcaggcgaa agtccaagct gcatcatgct caccgccgca
     1441 gccagtaata ttgcgcaaag cagataaggc agacaatccc gcttcagact gacgctcttg
     1501 cactccggtg tgggctgtgg tggtgtgccg ggcaaccgca gcagcatcag cagcgccagc
     1561 actggtgctg ccatcagtag ccccagtggc gccagaggat gaatggccag cattgctgcc
     1621 gcgcacagcg gcccgaatag cctgccgcaa ctcaggccgg agctgatggt tgccagagcg
     1681 gccatgcgat tcccttctcc agctctctgc aatgcccaga cctgacaggc tggcaccatt
     1741 gccgacacgg tcagcccgta ggcgatccgg gcgatgatta atattcccag tcccaccgct
     1801 gttgttatgg cgctggtagc catcagcacg cttcccagcc ctagcagggt aaagcttatg
     1861 ccatatcctc ccagagccca caacaccaca ggtttactgc cagtacggga aatttgcttt
     1921 ccccaccacg gggacgatgg cagaaacagc attgagccaa gcatcagcag ggcagcccat
     1981 acagacagac tcagatttgt ctgtatgacc agaacaggga gcacaaccag cagaccgttc
     2041 tgtccgatac cgagaagccc ggcactgaac gcaagtggcc agcaggacag tgttttttgg
     2101 ggcgtatctt cgatccctgg tgacattata atgtttcaac tccatgtatt aattgtgttt
     2161 atttgtaaaa ttaatttatc tgacaataac atttattgtt gataatgaga atcattatca
     2221 acataattgt tattatttta ctgtgtggga gctgtttgac tatgaccctg ccctctgaaa
     2281 aaccagccac agatgtggct gcgcagtgct tcctgaatgc actgattcgt gaaaccacag
     2341 actggaaact gacagaatac ccgccagacg aattgctcat cccgctggat gagcagaaat
     2401 cgctccattt cagagtggct tatttctccc caacccagca tcaccgcttt gcatttcctg
     2461 cacgtctggt cacggcatca ggcagttatc ctgtcgactt taccactctc tcccggctta
     2521 ttattgataa gctacgccat caactgtttc tgcccgttcc cctctgcgaa actttccacc
     2581 agcgcgtgct ggaaagccat gtccatacgc aacaggcaat tgatgcccgt catgactggg
     2641 ccgctctgcg tgaaaaagcg ttgaattttg gcgaggctga gcaggcgctg ctgacaggac
     2701 acgctttcca ccctgcgcct aagtctcatg aaccgtttaa ccgacgggag gctgaacgct
     2761 acctgcctga tatggcaccc cacttcccac tgcggtggtt ttcggtggat aaaacgcaaa
     2821 tcgccggtga aagtttgcat ctcaaccttc aacagcggct gacgcgattt gccgcagaga
     2881 atgcgcctca gttactcaac gaattaagtg acaaccaatg gctgttcccg ttgcacccgt
     2941 ggcaggggga atatcttttg cagcaggggt ggtgccaggc acttgttgct aaagggctga
     3001 ttaaagactt aggtgaggcc ggcacgtcgt ggctgccgac cacctcttcc cgttccctct
     3061 actgtgccac cagccgcgat atgatcaagt tctccctgag cgtacggctg accaactcca
     3121 tccgtactct gtccgtgaaa gaagtgaagc gtgggatgcg cctggcacgc ctggctcaaa
     3181 ccgacggctg gcagatgcta caggtccgct tcccgacttt ccgggtaatg caggaggatg
     3241 gctgggccgg gctgctcgat cttaacggca acatcatgca ggaaagtctg tttgccctgc
     3301 gtgaaaatct gctggtggat cagccgaaaa gccagactaa cgtactggtc tccctgactc
     3361 aggccgcacc tgatggcggt gattcgctgc tggtttcggc ggtaaaacgc ctgagcgatc
     3421 gcctcggtat cactgtgcaa caggccgccc atgcatgggt cgatgcatac tgtcagcaag
     3481 tgctaaagcc gctgtttacg gctgaagcgg attacggcct ggtgctgctg gcgcatcagc
     3541 aaaatattct tgtccagatg cttggggatc tgccggtcgg atttatttac cgtgactgtc
     3601 agggcagcgc ttttatgcct cacgcgacag actggctcga ttctattggc gaggcgcagg
     3661 cggaaaatat tttcacccat gagcagttgc tgcgctattt cccttattac ctgctggtta
     3721 actccacttt tgctgtgacc gctgcgctgg gggctgccgg gctggacagc gaatcgaatc
     3781 tgatggctcg tgtacgagca tcgctggctg aagtgcgtga tcaggtgact cataaaacct
     3841 gtctcaacta cgtgctggaa agtccgtact ggaacgtaaa aggtaacttt ttctgttatc
     3901 tgaacgatca taacgagaac accattgttg acccttcggt gatctatttc gatttcgcta
     3961 acccgctgca ggctcaggag gtctgaatgt ctgaggcaaa cattattcac agcagatatg
     4021 gactgcgctg tgaaaaactc gacaagcccc tgaatcttgg ctggggactg gacaatagcg
     4081 cggtgttgca ctgtcccggg gagctgccga cagggtggct gtgcgatgcg ctggatcaga
     4141 tatttatcgc cgcaccacaa ctgtcagcag tggctctccc ctgggccgaa tggcgtgagg
     4201 agccacaggc gctgacgctt tttgggcagg taaaaagcga cattatccat cgtactgcct
     4261 tctggcagtt accgttatgg ttgagttcac cggcaaaccg ggcctccggt gaaatggttt
     4321 ttgatgcaga gcgtgagatt tatttcccgc agcgcccacc ccgtccacag ggtgaggttt
     4381 accgccgtta cgatccacgt attcgcagga tgctgagttt ccgcattgcc gatcccgttt
     4441 ctgatgcaga acgtttcacc cgctggatga atgatccgcg cgttgagtat ttctgggagc
     4501 aaagtggctc actggaggta cagaccgcct atctggagcg ccagctcacc ggtaaacatg
     4561 cattcccgct gattgggtgc ttcgacgatc ggccattcag ctatttcgaa atctactggg
     4621 cggcggaaga ccgcattggt cgtcactatt cgtggcagcc ctttgaccgt ggcctgcatc
     4681 tgctggttgg tgaacagcaa tggcgcgggg cccactatgt gcaaagctgg ctgcgcgggc
     4741 tgacacatta cctgctgctg gatgagcccc gtacgcagcg cacagtactg gagccacgca
     4801 ccgataacca gcgcctgttc cgccatcttg agcctgcggg ataccggaca attaaagagt
     4861 tcgacttccc acataagcgc tcgcgcatgg tgatggcgga tcgtcatcac ttcttcacgg
     4921 aggtcggtct gtaatgaatc acaaggactg ggattttgtt aaccgtcggc tggtggcaaa
     4981 aatgttgtct gagatggagt atgagcaggt tttccacgcg gaatctcagg gcgatgacca
     5041 ctactgcatt aacctgccgg gagcacaatg gcgcttcatc gctgaacgtg gtatctgggg
     5101 ctggctgtgg attgatgccc aaacgttgcg ctgcacggac gaaccagtac tggcgcagac
     5161 gctgctgatg cagctaaagc cggtgctgtc aatgagtgat gccactgttg cagagcatat
     5221 gcaggatttg tatgccacgc tactgggcga cctgcaactg ctgaaggccc gtcgcggact
     5281 gagtgccagt gacctgatta atcttgatgc cgatcgtctg caatgcctgc tgagcggtca
     5341 tcctaaattc gtttttaata aaggtcgccg tggctggggc aaagaggcgc tggaacgata
     5401 tgcgccagag tataccaaca ccttcagact gcactggctg gcggtaaaac gtgaacatat
     5461 gatctggcgc tgcgataacg atctggatat tcagcagttg ttgactgccg caatggatcc
     5521 gcaggagttt acacgcttca gtcaggtctg gcaggaaaac gggctggatc acaactggct
     5581 accgctgccg gtacatccgt ggcagtggca gcaaaaaatc gccacagact tcatcgctga
     5641 ttttgtcgaa ggcaggatgg tgtctctcgg cgagtttggc gaccagtggc tcgcccagca
     5701 gtcgctgcgt accctgacca acgccagccg ccggggaggg ctggatatca agctgccgct
     5761 gaccatctac aacacctcat gctaccgggg aatccctggc agatatatcg ctgccggacc
     5821 actggcttca cgctggctgc aacaggtttt tgcgaccgac gccaccctgg tgcaaagcgg
     5881 cgcagtgatc cttggtgaac cggctgcagg ctatgtgtcc catgaaggct atgccgcgct
     5941 tgcccgggct ccctatcgct accaggaaat gcttggtgtt atctggcggg agaatccgtg
     6001 ccgctggctg aaaccggatg aaagtcctgt tctgatggca acactgatgg agtgcgacga
     6061 aaacaatcag ccgctggcag gcgcatatat cgaccgctcc ggactggacg ctgaaacctg
     6121 gcttacgcaa ttgttccggg tggttgtggt tccgctgtat cacctgcttt gccgctacgg
     6181 tgtcgcgctt attgcacacg gacaaaatat aacgctcgcc atgaaagagg gggtaccaca
     6241 gcgtgttctg ctgaaagact tccagggcga tatgcggctg gtgaaagaag cgtttcccga
     6301 aatggactct ttgcctcagg aggtgcgtga tgccacatcc cgtctgagtg cggactactt
     6361 aatccatgat ttgcagacgg gtcacttcgt gacagtactg cgttttattt cgccactgat
     6421 ggttcgtctt ggcgtacctg aaaggcgatt ttatcaactg ctggcagcag tgttgagtga
     6481 ttacatgaac aaacatccac aaatggcaga gcgttttgcg cttttctcac tcttcaggcc
     6541 acaaatcatt cgcgtggtgc tgaacccggt aaaactgacc tggccggacc tggatggcgg
     6601 cagtcgcatg ctgccgaatt accttgagaa tctgcaaaat ccgctgtggc tggtaactca
     6661 ggaatatgaa tcatgaaaaa aagtgtcgat tttattggtg tcgggacagg gccatttaat
     6721 ctcagcattg ctgcattgtc acatcagatc gaagaactga actgtctctt ctttgacgaa
     6781 catcctcatt tttcctggca tccgggtatg ctggtaccgg attgtcatat gcagaccgtc
     6841 tttctgaaag atctggtcag tgcagttgca cctacaaatc cctacagctt tgttaactat
     6901 ctggtgaagc acaaaaagtt ctatcgcttc cttacaagca ggctacgtac agtatcccgt
     6961 gaagagtttt ctgactatct ccgctgggct gctgaagata tgaataacct gtatttcagt
     7021 cataccgttg aaaatattga tttcgacaaa aaaagtcgat tgtttctggt tcagaccagt
     7081 cggggagaat attttgcccg caatatctgc cttggtacag gaaaacaacc ttatttacca
     7141 ccctgtgtga agcatgtgac acaatcctgt ttccatgcca gtgaaatgaa tcttcgtcgg
     7201 ccggacctga gtggaaaacg gataaccgtg gttggtggag gacagagtgg tgcagacctg
     7261 ttccttaatg cattacgcgg ggaatgggga gaagcggcgg aaataaactg ggtctcacgg
     7321 cgtaataatt ttaacgcact ggatgaggct gcttttgctg atgagtattt tacacctgaa
     7381 tatatttcag gcttctccgg actgaaggaa gatattcgcc atcagttact ggatgagcag
     7441 aaaatgacat cggatggcat cactgccgat tctttactga ccatttatcg tgagttgtac
     7501 caccgttttg aagttctgag aaaaccaaga aatatccgtc tgctacccag tcgctcggta
     7561 acaaccctgg aaagtagtgg tccgggctgg aggttattga tggaacatca tctggatcag
     7621 ggcagagaga gcctggaaag tgatgtggtg attttcgcca caggttaccg ttctgcattg
     7681 ccacaaatac ttccctcact gatgcccttg atcaccatgc acgataagaa cacctttaaa
     7741 gtgcgtgatg acttcactct ggaatggagt ggcccgaaag aaaataacat ctttgcggtc
     7801 aacgccagca tgcaaactca tggcatcgcc gaaccccagc tcagcctgat ggcctggcga
     7861 tctgcacgta ttcttaatcg cgtactgggg cgtgatttat tcgatctcag tatgccgccc
     7921 gcactgattc agtggcgcag cggcagccgg aaaaaaccgc agccggaggc tgcttcttta
     7981 actcactata cagcaaatat tcaggaataa cgatgatgcg caaaaagtat atgccccggg
     8041 ctcttggtcc gctgcttctt gtcgtgctgt caccagctgt cgcccagcaa aacgatgata
     8101 atgagatcat agtgtctgcc agccgcagca atcgaactgt agcggagatg gcgcaaacca
     8161 cctgggttat cgaaaatgcc gaactggagc agcagattca gggcggtaaa gagctgaaag
     8221 acgcactggc tcagttaatc cccggccttg atgtcagcag ccagagtcga accaactacg
     8281 gtatgaacat gcgtggccgc ccgctggttg tcctgattga cggtgtgcgc ctcaactctt
     8341 cacgttccga cagccgacaa ctggactctg tcgatccttt taatatcgac catattgaag
     8401 tgatctccgg cgcgacggcc ctgtacggtg gcgggagtac cggagggttg atcaacatcg
     8461 tgaccaaaaa aggtcagccg gaaaccatga tggagtttga ggctggcaca aaaagtggct
     8521 ttaacagcag taaagatcac gatgagcgca ttgccggtgc tgtctccggc ggaaatgacc
     8581 atatctccgg acgtctttcc gtggcatatc agaaatttgg cggctggttt gacggtaacg
     8641 gcgatgccac cctgcttgat aacacccaga ccggcctgca gcactccaat cggctggaca
     8701 tcatgggaac cggtacgctg aacatcgatg aatcccggca gcttcaactg ataacgcagt
     8761 actataaaag tcagggagac gacaattacg ggcttaatct cgggaaaggc ttttccgcca
     8821 tcagcgggag cagcacacca tacgtcagta aggggctgaa ttctgaccgc attcccggca
     8881 ctgagcggca tttgatcagc ctgcagtact ctgacagtga tttcctggga caggaactgg
     8941 tcggtcaggt ttactaccgc gatgagtcgt tgcggtacta cccgttcccg acggtaaatg
     9001 cgaataaaca ggcgacagct ttctcctcgt cacagcagga taccgaccag tacggcatga
     9061 aactgactct gaacagccaa ctgatggacg gctggcaaat cacctggggg ctggatgctg
     9121 agcatgagcg ctttacctcc aaccagatgt tcttcgatct ggctcaggca agtgcttccg
     9181 gagggctgaa caaccataag atttacacca ccgggcgcta tccgtcatat gacatcacca
     9241 atctggcggc cttcctgcaa tccagctatg acattaatga tatttttacc gttagcggtg
     9301 gcgtacgcta tcagtatact gagaacaggg tagatgattt catcgactac acgcagcaac
     9361 agaagattgc tgccgggaag gcgatatctg ccgacgccat tcctggtggt tcggtagatt
     9421 acgataactt tctgttcaat gctggtctgc tgatgcacat caccgaacgt cagcaggcat
     9481 ggttcaattt ttcccagggg gtggcattgc cggatccggg gaaatattat ggtcgcggca
     9541 tctatggtgc agcagtgaac ggccatcttt ccctgacaaa gagcgtgaac gtcagcgaca
     9601 gtaagctgga aggcgtgaaa gtcgattctt atgaactggg ctggcgcttt accggtgaca
     9661 acctgcggac tcaaatcgcg gcatattact cgctttccaa taagagcgtg gaaaggaata
     9721 aagatctgac catcagtgtg aaggacgaca ggcgccgtat ttacggcgtg gaaggtgcgg
     9781 tggacttcct gatcccggat actgactgga gtaccggtgt gaacttcaat gtgctgaaaa
     9841 ccgagtcgaa agtgaacggt caatggcaaa aatatgacgt gaaggaatca agtccatcga
     9901 aagcgacagc ttacattaac tgggcgccgg aaccgtggag tctgcgtgta cagagcacca
     9961 cttctttcga cgtaagcgat gcagagggta acgatattaa tggttacact accgtcgatt
    10021 ttatcagtag ttggcagctt ccggtgggaa cactcagctt cagcgttgag aacctcttcg
    10081 accgtgacta taccactgtc tggggacagc gtgcacctct gtactactgc ccgggttacg
    10141 gccctgcttc actgtacgac tacaaaggcc ggggccgaac ctttggtctg aactactcag
    10201 tgctgttctg acggggatgc ttttgcaaca agggtatgct gtaaacaaca tggtccttgg
    10261 tggccatgtt gtttacagcc agtataatat acagccttaa caagatattc cgtctccgga
    10321 atggactgtt caccactgaa taacattatt tctgttttat ggatctttga ctgaaattta
    10381 tcgcatatcc tcatttcatt gtccgtctga aaactaacct tacaagtaag cgtcgtctca
    10441 gcaccgtctg gcagatcctg aaattcctga gaggatgatc tgcgcccaat atcctgagcc
    10501 ggaactttgc tgcaatgaag gccaacgaaa aatgggggag tatgttatag agtacttgct
    10561 acagggtaaa aagaggtacc tgtcgctggt tctccgttca gtttaaccgg gagattttct
    10621 cctgcgcctg tcataaagtc cggtaatgga gattgttaat acgatggtgc acgatgcatt
    10681 gtcggtgccc ggcccggagg atgccccgta actacatacg gatcagggtt ggcaatatcg
    10741 aatggcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat gccacacaaa
    10801 ggaaacggtc tgaataacgc agtgatggag aacttcttcg gcacactgca aaatcgcatc
    10861 gtggtcgatc gctaattcgc cgtgaatcac tgaacgatgt tccatgcgaa cagatattcg
    10921 atcatatcga aatattaaac attacccctc cccgcattgg gcaaaaaaaa aacaaaaaac
    10981 gatcaattag tattgattca cataatctct catcattaat gagaacattg tcacatattt
    11041 acaatataga actgttactt ttagtaagct gttagtaacc aactgttact aataatttta
    11101 attcttaata ctttgttcat tcaataaatg agttgagag
//
LOCUS       DAZPJR010000140        11003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPJR010000140 DAZPJR010000000
VERSION     DAZPJR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.142"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..280)
                     /locus_tag="ACHVB7_003318"
     CDS             complement(<1..280)
                     /locus_tag="ACHVB7_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1915221.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(447..1187)
                     /locus_tag="ACHVB7_003319"
     CDS             complement(447..1187)
                     /locus_tag="ACHVB7_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR1915222.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1280..1915)
                     /gene="argO"
                     /locus_tag="ACHVB7_003320"
     CDS             complement(1280..1915)
                     /gene="argO"
                     /locus_tag="ACHVB7_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR1915223.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="ACHVB7_003321"
     CDS             complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="ACHVB7_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR1915224.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            3110..3208
                     /locus_tag="ACHVB7_003322"
                     /pseudo
     CDS             3110..3208
                     /locus_tag="ACHVB7_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="ACHVB7_003323"
     CDS             complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="ACHVB7_003323"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR1915225.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="ACHVB7_003324"
     CDS             complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="ACHVB7_003324"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR1915226.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5779..6798)
                     /gene="epd"
                     /locus_tag="ACHVB7_003325"
     CDS             complement(5779..6798)
                     /gene="epd"
                     /locus_tag="ACHVB7_003325"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR1915227.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7170..7601
                     /locus_tag="ACHVB7_003326"
     CDS             7170..7601
                     /locus_tag="ACHVB7_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR1915228.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7624..8202
                     /locus_tag="ACHVB7_003327"
     CDS             7624..8202
                     /locus_tag="ACHVB7_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR1915229.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8203..8910
                     /locus_tag="ACHVB7_003328"
     CDS             8203..8910
                     /locus_tag="ACHVB7_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR1915230.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RQAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8898..9575
                     /locus_tag="ACHVB7_003329"
     CDS             8898..9575
                     /locus_tag="ACHVB7_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1915231.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9569..10246
                     /locus_tag="ACHVB7_003330"
     CDS             9569..10246
                     /locus_tag="ACHVB7_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1915232.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10218..10931)
                     /locus_tag="ACHVB7_003331"
     CDS             complement(10218..10931)
                     /locus_tag="ACHVB7_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR1915233.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10928..>11003)
                     /locus_tag="ACHVB7_003332"
     CDS             complement(10928..>11003)
                     /locus_tag="ACHVB7_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1915234.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2627 a         2920 c         2740 g         2716 t
ORIGIN      
        1 ctcgtgaacg acgccctgag ttatggattt cattatcaat cgcagaaagt cgttgataaa
       61 ccgggaacag tttttgatac agttcctgac cggctttatt gaggctgata ttattatctt
      121 tgcgggtaaa tagcgtatag ccaatttgtt cttccagcgc ggcaatgctt ttaccaaatg
      181 gcgaagccgt catatggatt ttttctgccg cccgggcaat attattggtt tgcgctaata
      241 aaatgaaatt gcgcatcttt ttcgaaataa aaatgtccat cccccctccc cgcatccttg
      301 tccgcttaac cgtttcagtc agcctatcct tgatgaaacc gcgagcaaag ataggtgatt
      361 acgtcatggt tttacagaaa attacagaaa aaggaggcaa tatcgggtaa aggcattagc
      421 ccgacgaata cgtcgggcta caagtattat tgtgctgcag gtgttttagc gggttgttga
      481 tccacaggtt ctaactggaa gaccacatcg acctgatcat caaactgaat agcggcctgc
      541 tcgtaagttt cctgggcaga caccggcgcg gcatcggctt tcatcatccg caccattggg
      601 ctgggctgat agttggaaac atggtagcgc acgctatata ccggccccag tttacgatga
      661 aagccgttcg ccagttcctg cgcctgatga atcgcgttat caatcgctgc cttacgcgct
      721 ttgtctttat aggcatccgg ctgcgccacg cccagcgaca cagaacgaat ttcgttaaga
      781 cccgccttca gcgcgccatc cagcaaggaa ttcagtttgt ctaactgacg gagcgtgact
      841 tccaccgttc tcacagcgcg gtagcctttc aggatacttt taccatcctg ataatcataa
      901 tctggctggg tgcgtaagtt cgctgagctg atatcttttt tcgcgatctg attgagttca
      961 aggaaggaaa tgtattgtgc gacgcgctca tctgcctgtt tcttggctgt agcggcatcc
     1021 ttcgcggcca cgttaacttc aatcgcaaga gtggcaatgt ctggcaccgc atccacactt
     1081 gccgtaccgg aggtgacaat atgcggtcca tccggcaatt cgttagcctg cgctgccatc
     1141 ccgctaatac ccattaatgc cgccagggcg ataactttga acttcacgtt agtctctccg
     1201 tgttaattga cttgcccaaa attcaggacc tctggaggca agcttagcgc ctctgtttta
     1261 tttttccatc agatagtgcc taactgaaca aggcttgtgc atgagcaata ccgtctctcg
     1321 ccagctgcaa ggctataaac cacatgacac atcccactac cagattgata atgcgctgtg
     1381 attttgccgt gcgcaggcgc ggtgccagcc aggctgcgag aatagccaga ccaaagaacc
     1441 acaggaaaga ggcgctaatt gtcccgagcg caaaccaacg ttttggttcg acgtcaagct
     1501 gcccgccaag gctgcccagt acaacaaaag tatccaggta aacatgcgga ttcagccagg
     1561 tcactgccag catggtggcg ataattttcc atctgccttg cttcaggact tcggcactag
     1621 ctaactcaat attactactc atcgctgttt taaaagcgcc aaaaccatac cacagcaaga
     1681 aggctacgcc gccccaggtg accagcgcca gcaaccacgg cgactgcatc aataacgcgc
     1741 tgccaccaaa aatcccggcg caaatcagga ccaaatcgct gatagcacaa agtaaggcaa
     1801 tcataatgtg gtactgacga cgaatgccct gattcatcac aaaagcattt tgtggaccga
     1861 gcggcagaat catagctgcc ccaagagcaa taccttgaaa gtaataagaa aacacgttgg
     1921 atattccgaa ttaagtatct gatgcagaga ctatatcgcg ggattgttat tagcggaaat
     1981 tgataatatt aattggcaat cagaataact aataagagag aggccagaga gtattatctg
     2041 gcctcagcgt tgattacgca gctttgtctt ctttcacccg cttaaagttc acatccattt
     2101 gcgggtacgg gaagctgata ccggcggcat caaattcacg tttaatacgc tccagcacat
     2161 cccagtacac gttttgcaga tcgccgctgt tgctccagac gcggaccacg aaattaatcg
     2221 acgatgcacc aagttcgttc agacgcacag tcatttcgcg atctttcaaa atgcgatctt
     2281 cagactggat aatattggtc aggatctgct taacctgatc gatatcggaa tcatacgcca
     2341 cgccaataat aaattcgtta cggcgaactg gctcgcgaga gaagttaata atatttccgg
     2401 caataatttt accgttcgga ataacgataa ttttaccgtc tgcagtacgc atggtggtgg
     2461 agaaaatctg cacactcagc acagtaccgg ctacgccgcc gagatcaaca tattctccgg
     2521 cacggaacgg gcggaacatg acaagtaaca cgccagcggc caggttagaa agtgacccct
     2581 gcaaagccag accaacagct aagcctgcgg caccgagtac agcaatgact gacgctgttt
     2641 gtacacccac gcgtcccagg gcagcgatta gcgtaaaggc gataataccg taacggacta
     2701 atgcagaaag aaaatcagca acagtggcat cgattttacg ggagatcatc aggcgattca
     2761 ccgcgttgga aatcatccgc gcgataatca aaccaacgat gatgatcgcg agtgccgcca
     2821 cgatgtttac tgcataactt agcagcagcg cctggttagc taccagccag cttcccgcgc
     2881 cgtttatgct atcgacaaca ttcaaatctt ccattcaata ttccttttca actgactcaa
     2941 aatggagaaa tacactccgc cgccttatga cgggcagtct gacaagggta aacaataaag
     3001 cataattttg ccaaatagat cacagatttc tcatctgaat caccttgctt ttggcgaaaa
     3061 aatccccgca gagcggggct cagacagata tcaatgtgcg ctttgtcatg ccggatgcgg
     3121 cgtgaacgcc ttatccggcc tacaaaaacc tgcaaattca atatattgca ggatacgtgt
     3181 aggcctgata agcgtagcgc atcaggcaat tttgcttttg gcgaaaaaaa gacccgcaga
     3241 gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg ttcagttcct
     3301 ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc catacgcgcg
     3361 gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccaac tgcccctgca
     3421 gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc cattgggtat
     3481 cggtatcgat gttcattttt actacgccgt agcttacgga gtctttgatt tcctgagcag
     3541 tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg ttgtgtttct
     3601 tggaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg ttacccggct
     3661 tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg ctgattttgc
     3721 tcagttcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca gaagcgtcca
     3781 tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg atttccagag
     3841 tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg ttctcttgca
     3901 gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg gtagctgcga
     3961 agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc ttcgcgcagt
     4021 ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga acgtgatgcg
     4081 caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc acgcctttac
     4141 cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct ttaactttag
     4201 cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact gctggcagtg
     4261 cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca gtgattacgc
     4321 caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt cgggccttat
     4381 actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact
     4441 tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa ttatttagag
     4501 tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc ctgccctgtg
     4561 attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac tttaccttcc
     4621 acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc agcaatgccg
     4681 aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc ttcgctgtct
     4741 gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc gaacacgcca
     4801 accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag ttcctgagcg
     4861 gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac agatttcagg
     4921 gtagccgatg cagtttcaga gaactcggtt gctacgcgaa catcagacgg aaccgggatg
     4981 ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta cagggattta
     5041 cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc aacaatcagc
     5101 tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac tttagaacca
     5161 ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag cgcgtccagt
     5221 tcagctgcca acagcgggcc tgcgcacgca acatcagcga atttaccgat accgtgagta
     5281 gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc acacagtgca
     5341 gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa gcgaacgttt
     5401 tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc tttaaccaga
     5461 cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag agagaattct
     5521 tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac tttcgcgcct
     5581 tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc gctggttact
     5641 ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt cccagcaaga
     5701 tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc taaaagtttt
     5761 gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg tcgtgtcgag
     5821 cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga tcaggtgtgc
     5881 gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt ggttaaaatc
     5941 tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac cttgtgctgc
     6001 tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg tcacgcttaa
     6061 atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa agcgatcgtt
     6121 aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg gaatgatcga
     6181 ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa cctgttgatc
     6241 gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg catcatctaa
     6301 caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa cgatgcggtg
     6361 ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga gatcgttact
     6421 gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt cgccatgctc
     6481 gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt cacgccaggg
     6541 gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa caaaaagttg
     6601 atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat atttcaacaa
     6661 atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa tttccgcccg
     6721 gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga agccatttat
     6781 cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt tgccagacag
     6841 agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac tgattggtta
     6901 attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac gtggaataaa
     6961 aggaatgatt gtccagtcaa agaaaacaat tatctgactt acgtcacgtt ttcagccccg
     7021 cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa atcttttcgt
     7081 tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc gatattatcc
     7141 gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca tattcttccc
     7201 ccgcacgctt ttatgcactg cgcctcctcc ccgggcagga agttttttcc cagctacacg
     7261 cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc ggtagcctga
     7321 ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctccttaac ggtacattcg
     7381 aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat ctctgcgttt
     7441 ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc gtgcggacca
     7501 cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa ccttgcgcgc
     7561 tttctggtta cgacgagttg catatttccc ccgttaaata actactttca aagaggtttt
     7621 cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat atctatcgcc
     7681 atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt tctcgactcc
     7741 atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct gaccggctta
     7801 ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt tgcacccgtc
     7861 gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt tcgcaccctg
     7921 ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt cgctgtaccg
     7981 ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt cgttgcctgg
     8041 atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacagtgat aggcgcgaac
     8101 ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca gctgccgatt
     8161 cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc atttacctca
     8221 ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca gaccattttg
     8281 ccgatctata gcgcggcgac ctttttttgt cttattgcgc taaaggccac gcggcggcag
     8341 gcaaaatatg tcgcctggct gatgttctcg ctgggagccg ggctgtggct ggtacacggt
     8401 ggctggctaa cagaatggct aagcggcacg ccgcgttcgc cggagcgttg gacgcatgct
     8461 attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg gatgcagtac
     8521 gtccccgtgc aatgttttat ccgcgctctg tttgcttctc gcctgccgcc aggcgtcgct
     8581 tatctgtttg ccggaccgtt attggtcgtt gagcagttaa aacggcaatt agcgattatt
     8641 catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca gcgtttacgt
     8701 gcgatgcccg cgttaattat tctgctaaca cacaatgcgc ttaacgatct ggctgtccgt
     8761 ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac gttgtgggca
     8821 ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct gatgctaaca
     8881 gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg acacactcaa
     8941 cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc gccatctttg
     9001 gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg tatccggact
     9061 ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt gggcatctcc
     9121 cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat ttgcagctct
     9181 ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat ctgtgtcttg
     9241 ctgaaacaga gattatggcg cggattgatg ccgccctgac tctgaccgag tgccagccgt
     9301 tgcgccatcg tcacccggca acactctccg gtggagagac acaacgcgtg gtcatcgcct
     9361 gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagt cgcctgacgc
     9421 cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa cgtggttcgc
     9481 tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag caagcgtggc
     9541 aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat cttatcgctg
     9601 gcctagcgca gcaacggatt gtctttgcga catttctctg caactcaaac aaggtgaatg
     9661 gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag taatggcagg
     9721 tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga aaaacctgaa
     9781 aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa accaactttt
     9841 tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat gcccggcgga
     9901 tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag acactgccaa
     9961 ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg cctgcctgga
    10021 agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg acgaaaattg
    10081 gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga gcgtggtagc
    10141 aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc gactggaaga
    10201 tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt catcattaag
    10261 gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc gtcggtacgg
    10321 tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg ccccaccaga
    10381 cgctcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca aaattgcgca
    10441 agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat gacgagtggt
    10501 gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta ctgcggccac
    10561 gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac gtcaaatgtc
    10621 tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt gtaatggtga
    10681 aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg cacgagatat
    10741 tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag aaaaaccacc
    10801 gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa cggtttatgc
    10861 acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt caccgttaat
    10921 tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc agcgccgttt
    10981 tgaccattcc gctaaaacgc gcc
//
LOCUS       DAZPJR010000141        10929 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPJR010000141 DAZPJR010000000
VERSION     DAZPJR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.143"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHVB7_003333"
     CDS             complement(<1..312)
                     /locus_tag="ACHVB7_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR1915235.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHVB7_003334"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHVB7_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR1915236.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHVB7_003335"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHVB7_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR1915237.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHVB7_003336"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHVB7_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR1915238.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHVB7_003337"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHVB7_003337"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR1915239.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHVB7_003338"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHVB7_003338"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR1915240.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHVB7_003339"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHVB7_003339"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR1915241.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHVB7_003340"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHVB7_003340"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR1915242.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHVB7_003341"
     CDS             complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHVB7_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR1915243.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..>10929)
                     /gene="lpfC-O113"
                     /locus_tag="ACHVB7_003342"
     CDS             complement(9376..>10929)
                     /gene="lpfC-O113"
                     /locus_tag="ACHVB7_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR1915244.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2851 a         2830 c         2479 g         2769 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccacccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaattaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcacgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctggagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggtagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 agtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttac
//
LOCUS       DAZPJR010000142        10898 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPJR010000142 DAZPJR010000000
VERSION     DAZPJR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.144"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..611
                     /locus_tag="ACHVB7_003343"
                     /pseudo
     CDS             1..611
                     /locus_tag="ACHVB7_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /gene="prfH"
                     /locus_tag="ACHVB7_003344"
     CDS             608..1222
                     /gene="prfH"
                     /locus_tag="ACHVB7_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR1915245.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="ACHVB7_003345"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="ACHVB7_003345"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1915246.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="ACHVB7_003346"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="ACHVB7_003346"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1915247.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="ACHVB7_003347"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="ACHVB7_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1915248.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="ACHVB7_003348"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="ACHVB7_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1915249.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="ACHVB7_003349"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="ACHVB7_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1915250.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="ACHVB7_003350"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="ACHVB7_003350"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1915251.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="ACHVB7_003351"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="ACHVB7_003351"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1915252.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="ACHVB7_003352"
     tRNA            9115..9190
                     /locus_tag="ACHVB7_003352"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9796..9954
                     /locus_tag="ACHVB7_003353"
     CDS             9796..9954
                     /locus_tag="ACHVB7_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915253.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2760 a         2575 c         2778 g         2785 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatagcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt aacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcataa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttcaggtga
    10141 tttaagggta ttaaacttga tttaattgat taaaatataa acagataatt atgtatctta
    10201 ttgatttata aggttgtttt taagtaaagg taaataaaaa ataaatagcc ataaatagct
    10261 ataaatacat cttaatacct atatcccttc ccttatggct agattgcgag aggtgcttaa
    10321 cgcgattttc agcgcccccc gatctggaaa atccagaatc cccaaatatc agcaaggaat
    10381 tttcttgcgt aatcaacatc gtttttacct attaaatcat tacacagaaa gcctttctgt
    10441 aatgtctctc actaaacttt cactaaacac gagaatgatc ggttttatgt cggatcagat
    10501 gctagaaaca gcaccacgtc tcacaagggc tgtaagcgat gaaaccagtg tttatgcggg
    10561 tgcaggtcaa aatataggcc aaaatccatt taacataata aatgttatat gtgctaagga
    10621 acacatataa cgccttgaat tgatgtatca gtggaaaagt gatggatttt ttacgtagga
    10681 gcacgaaagg gcttctattg acttttgtgt gctatgaaat ataattaaag ttttttgttt
    10741 ttattgtgtg atgtggtggg ttacacaggg gaatagatta aatggtgttc tgtttataca
    10801 ggtttatttg ttagatcaac aatttggcat ttctaaacag atgtttgaac gttgccaaat
    10861 gtcttgacaa cacaagaaaa gggcttgcta tgccaggc
//
LOCUS       DAZPJR010000143        10856 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPJR010000143 DAZPJR010000000
VERSION     DAZPJR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.145"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            83..424
                     /locus_tag="ACHVB7_003354"
     CDS             83..424
                     /locus_tag="ACHVB7_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR1915254.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(415..1341)
                     /locus_tag="ACHVB7_003355"
     CDS             complement(415..1341)
                     /locus_tag="ACHVB7_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1915255.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            1431..2429
                     /locus_tag="ACHVB7_003356"
     CDS             1431..2429
                     /locus_tag="ACHVB7_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR1915256.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2426..2644)
                     /locus_tag="ACHVB7_003357"
     CDS             complement(2426..2644)
                     /locus_tag="ACHVB7_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR1915257.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="ACHVB7_003358"
     CDS             complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="ACHVB7_003358"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR1915258.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="ACHVB7_003359"
     CDS             complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="ACHVB7_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR1915259.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5948..6709
                     /gene="cysZ"
                     /locus_tag="ACHVB7_003360"
     CDS             5948..6709
                     /gene="cysZ"
                     /locus_tag="ACHVB7_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR1915260.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6894..7865
                     /gene="cysK"
                     /locus_tag="ACHVB7_003361"
     CDS             6894..7865
                     /gene="cysK"
                     /locus_tag="ACHVB7_003361"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR1915261.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8249..8506
                     /gene="ptsH"
                     /locus_tag="ACHVB7_003362"
     CDS             8249..8506
                     /gene="ptsH"
                     /locus_tag="ACHVB7_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR1915262.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8551..10278
                     /gene="ptsI"
                     /locus_tag="ACHVB7_003363"
     CDS             8551..10278
                     /gene="ptsI"
                     /locus_tag="ACHVB7_003363"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR1915263.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10319..10828
                     /gene="crr"
                     /locus_tag="ACHVB7_003364"
     CDS             10319..10828
                     /gene="crr"
                     /locus_tag="ACHVB7_003364"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR1915264.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2640 a         2798 c         2694 g         2724 t
ORIGIN      
        1 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctgccg atattacctc
       61 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
      121 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcaaaaa tcgcgcggat
      181 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
      241 tgctgaagac aaacaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
      301 acaactggcg cagttgcaga gacagcaggc agaaaaggcg caggagaaag agcagcgttt
      361 gtctctgaat gtatcccttc taaatcctgt tgaaaatacg actcatatcg atatctatat
      421 ctgatacaac ggagcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
      481 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
      541 ctggcgttca acaaccggcg taatgcgctt taccaccagt gggctacctt caggcaatgg
      601 tagcgccagt gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
      661 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
      721 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
      781 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
      841 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
      901 gatgcaaaaa tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
      961 gtgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     1021 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
     1081 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
     1141 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     1201 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
     1261 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     1321 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     1381 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     1441 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     1501 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     1561 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
     1621 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     1681 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     1741 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     1801 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
     1861 caccattgct agttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg
     1921 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
     1981 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
     2041 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
     2101 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
     2161 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
     2221 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
     2281 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
     2341 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
     2401 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
     2461 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
     2521 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac
     2581 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
     2641 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
     2701 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
     2761 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
     2821 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
     2881 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
     2941 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
     3001 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cacttccagc
     3061 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
     3121 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
     3181 tgtgccgatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
     3241 agatctgccg gagtgtggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
     3301 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
     3361 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
     3421 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
     3481 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
     3541 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
     3601 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
     3661 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
     3721 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
     3781 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
     3841 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
     3901 ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
     3961 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
     4021 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
     4081 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
     4141 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
     4201 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
     4261 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
     4321 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
     4381 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
     4441 cgtatctggc tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
     4501 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
     4561 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc
     4621 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
     4681 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
     4741 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
     4801 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
     4861 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
     4921 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
     4981 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
     5041 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
     5101 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
     5161 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
     5221 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
     5281 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
     5341 ctgcatcggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
     5401 ctggatcggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga
     5461 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
     5521 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
     5581 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
     5641 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
     5701 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
     5761 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
     5821 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
     5881 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
     5941 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
     6001 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
     6061 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
     6121 cccgactctc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
     6181 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
     6241 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
     6301 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
     6361 cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
     6421 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
     6481 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
     6541 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
     6601 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
     6661 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
     6721 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
     6781 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
     6841 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
     6901 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
     6961 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
     7021 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
     7081 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
     7141 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
     7201 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
     7261 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
     7321 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
     7381 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
     7441 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
     7501 ttgagccaac cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg
     7561 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
     7621 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
     7681 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga
     7741 aactacaaga agatgaaagc tttaccaata agaatattgt ggttattcta ccatcatcgg
     7801 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
     7861 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
     7921 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
     7981 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
     8041 tttatgattt ggttcaattc ttcctttaac ggcataatgt ttaatgacgt acgaaacgtc
     8101 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
     8161 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
     8221 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
     8281 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
     8341 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
     8401 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
     8461 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
     8521 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
     8581 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
     8641 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
     8701 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
     8761 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
     8821 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
     8881 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
     8941 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
     9001 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
     9061 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
     9121 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
     9181 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
     9241 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
     9301 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
     9361 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
     9421 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
     9481 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
     9541 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
     9601 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
     9661 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
     9721 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
     9781 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
     9841 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
     9901 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
     9961 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    10021 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    10081 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    10141 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    10201 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    10261 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    10321 gggtttgttc gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat
    10381 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    10441 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    10501 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    10561 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    10621 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    10681 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    10741 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    10801 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgccca
//
LOCUS       DAZPJR010000144        10841 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPJR010000144 DAZPJR010000000
VERSION     DAZPJR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.146"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHVB7_003365"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHVB7_003365"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR1915265.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHVB7_003366"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHVB7_003366"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR1915266.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHVB7_003367"
     CDS             complement(2698..3390)
                     /locus_tag="ACHVB7_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR1915267.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHVB7_003368"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHVB7_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR1915268.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5686..6543
                     /gene="focA"
                     /locus_tag="ACHVB7_003369"
     CDS             5686..6543
                     /gene="focA"
                     /locus_tag="ACHVB7_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR1915269.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6598..8880
                     /gene="pflB"
                     /locus_tag="ACHVB7_003370"
     CDS             6598..8880
                     /gene="pflB"
                     /locus_tag="ACHVB7_003370"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1915270.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9072..9812
                     /gene="pflA"
                     /locus_tag="ACHVB7_003371"
     CDS             9072..9812
                     /gene="pflA"
                     /locus_tag="ACHVB7_003371"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR1915271.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9944..10081)
                     /locus_tag="ACHVB7_003372"
                     /pseudo
     CDS             complement(9944..10081)
                     /locus_tag="ACHVB7_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            10181..>10841
                     /locus_tag="ACHVB7_003373"
     CDS             10181..>10841
                     /locus_tag="ACHVB7_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1915272.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         2791 a         2712 c         2568 g         2770 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgctttac atatatttgc attaataaaa taattgtaat tataaggtta
     5401 aatatcggta atttgtattt aataaatacg atcgatattg ttactttatt cgcctgatgc
     5461 tcccttttaa ttaactgttt tagcggagga tgcggaaaaa attcaactca tttgttaatt
     5521 tttaaaattt atttttattt ggataatcaa atgtttactc cgtatctgca taaaaaccat
     5581 gcgagttacg ggcctataag ccaggcgaga tatgatctat atcaatttct catctataat
     5641 gctttgttag tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa agctgacaac
     5701 ccttttgatc ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat
     5761 aaagcaacga aacatccgct taagactttc tatctggcga ttaccgccgg tgttttcatc
     5821 tcaatcgcat tcgtcttcta tatcacagca accactggca caggcacaat gcccttcggc
     5881 atggcaaaac tggttggcgg catttgcttc tctctggggc tgattctttg tgttgtctgc
     5941 ggagccgatc tctttacttc caccgtgttg attgttgttg ctaaggcgag tgggcgcatc
     6001 acctggggtc agttggcgaa aaactggcta aatgtctatt ttggcaacct ggtcggcgca
     6061 ctgctgtttg tacttttaat gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg
     6121 ggactaaacg tcctacaaac cgccgaccac aaagtgcacc atacttttat tgaggccgtc
     6181 tgtcttggta tcctggcaaa cctgatggta tgtctggcag tatggatgag ttattctggc
     6241 cgcagcctga tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt tgttgccagc
     6301 ggttttgagc acagtatcgc aaacatgttt atgatcccga tgggtattgt aatccgcgac
     6361 ttcgcatccc cggaattttg gaccgcagtc ggttctgcac cggaaaattt ttctcacctg
     6421 accgtgatga atttcatcac tgataacctg attccggtta cgatcggcaa cattatcggt
     6481 ggtggtttgt tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa cgaccaccat
     6541 taatggttgt cgaagtacgc agtaaataaa aaatccactt aagaaggtag gtgttacatg
     6601 tccgagctta atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg tgactggcag
     6661 aatgaagtaa acgtccgtga cttcattcag aaaaactaca ctccgtacga gggtgacgag
     6721 tccttcctgg ctggcgctac tgaagcgacc accaccctgt gggacaaagt aatggaaggc
     6781 gttaaactgg aaaaccgcac tcacgcgcca gttgactttg acaccgctgt tgcttccacc
     6841 atcacctctc acgacgctgg ctacatcaac aagcagcttg agaaaatcgt tggtctgcag
     6901 actgaagctc cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt
     6961 tcctgcaaag cgtacaaccg cgaactggat ccgatgatca aaaaaatctt cactgaatac
     7021 cgtaaaactc acaaccaggg cgtgttcgac gtttacactc cggacatcct gcgttgccgt
     7081 aaatctggtg ttctgaccgg tctgccagat gcatatggcc gtggccgtat catcggtgac
     7141 taccgtcgcg ttgcgctgta cggtatcgac tacctgatga aagacaaact ggcacagttc
     7201 acttctctgc aggctgatct ggaaaacggc gtaaacttgg aacagactat ccgtctgcgc
     7261 gaagaaatcg ctgaacagca ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac
     7321 ggctacgaca tctctggtcc ggctaccaac gctcaggaag ctatccagtg gacttacttc
     7381 ggctacctgg ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc
     7441 accttcctgg atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa
     7501 gcgcaggaaa tggttgacca cctggtcatg aaactgcgta tggttcgctt cctgcgtact
     7561 ccggaatacg atgaactgtt ctctggcgac ccgatctggg caaccgaatc tatcggtggt
     7621 atgggcctcg acggtcgtac cctggttacc aaaaacagct tccgtttcct gaacaccctg
     7681 tacaccatgg gtccgtctcc ggaaccgaac atgaccattc tgtggtctga aaaactgccg
     7741 ctgaacttca agaaattcgc cgctaaagtg tccatcgaca cctcttctct gcagtatgag
     7801 aacgatgacc tgatgcgtcc ggacttcaac aacgatgact acgctattgc ttgctgcgta
     7861 agcccgatga tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa
     7921 accatgctgt acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg
     7981 aagtctgaac cgatcaaagg cgatgtcctg aactatgatg aagtgatgga gcgcatggat
     8041 cacttcatgg actggctggc taaacagtac atcactgcac taaacatcat ccactacatg
     8101 cacgacaagt acagctacga agcctctctg atggcgctgc acgaccgtga cgttatccgc
     8161 accatggcgt gtggtatcgc tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa
     8221 tatgcgaaag ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa
     8281 ggcgaatacc cgcagtttgg taacaacgat ccgcgtgtag atgacctggc tgttgacctg
     8341 gtagaacgtt tcatgaagaa aattcagaaa ctgcacacct accgtgacgc tatcccgact
     8401 cagtctgttc tgaccatcac ttctaacgtt gtgtatggta agaaaacggg taacacccca
     8461 gacggtcgtc gtgctggcgc gccgttcgga ccgggtgcta acccgatgca cggtcgtgac
     8521 cagaaaggtg cagtagcctc tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa
     8581 gatggtatct cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagtt
     8641 cgtaagacca acctggctgg tctgatggat ggttacttcc accacgaagc atccatcgaa
     8701 ggtggtcagc acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa
     8761 aacccggaaa aatatccgca gctgaccatc cgtgtatctg gctacgcagt acgtttcaac
     8821 tcgctgacta aagaacagca gcaggacgtt attactcgta ccttcactca atctatgtaa
     8881 ttagatttga ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac gaaagtgggg
     8941 ccttttttag cacgagagcc ttttttgtca gctatctata ctctaaggtg actgccaaaa
     9001 cagactcgac gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga
     9061 gaaacaccgc aatgtcagtt attggtcgca ttcactcctt tgaatcctgt ggaaccgtag
     9121 acggcccggg tattcgcttt atcacctttt tccagggctg cctgatgcgc tgcctgtatt
     9181 gtcataaccg cgacacctgg gatacgcatg gcggtaaaga agttaccgtt gaagatttaa
     9241 tgaaagaggt ggtgacctat cgccacttta tgaacgcttc cggcggcggc gttaccgcat
     9301 ccggcggtga ggcaatccta caagctgagt ttgttcgtga ctggttccgc gcctgcaaaa
     9361 aagaaggcat tcatacctgt ctggacacca acggttttgt tcgtcgttac gatccggtga
     9421 ttgatgaact gctggaagta accgacctgg taatgctcga tctcaaacag atgaacgacg
     9481 agatccacca aaatctggtt ggagtttcca atcaccgcac gctggagttc gccaaatatc
     9541 tggcgaacaa aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg
     9601 atgacgattc agcgcatcgc ctcggtgaat ttacccgtga tatgggcaac gttgagaaaa
     9661 tcgagcttct cccctaccac gagctgggca aacacaaatg ggtggcaatg ggtgaagagt
     9721 acaaactcga cggtgttaaa ccaccgaaga aagagaccat ggaacgcgtg aaaggcattc
     9781 ttgagcagta cggtcataag gtaatgttct aatttccccg taaagcggca actttattga
     9841 gttgccgctt ttttattccg cacttcattc tgctacagcg tttttaacac atcttatagt
     9901 gcatctacca tatcccgtac ctgagataat aacgactgat aatattacgc cgatataaat
     9961 cgagttccgt cacctgtatt ttccgctcca ttgcccgctg atggatcgca tcagcaatat
    10021 gtgcggtcaa tgaatcatga cgaggatggg cccataccaa ataaatacgt tcagactgca
    10081 taattttacc tccacgttaa gttctggagc gcactttacg caggcggcat aataatgtaa
    10141 acgtgatgat tcaggatgag ttaatgaagg attttcacta atgcggatga atatgtctga
    10201 ctttgccact ttctttgctg tggcccgtaa tcaaagcttt cgtgcagcgg gcgatgagtt
    10261 aggcttatcc tcgtccgcca ttagccatag tattaaaaca ctggaacaac gtcttaaaat
    10321 tcgcttattc aatcggacaa cccgaagcgt atcgttaacg gaagcgggtt ctaatctcta
    10381 tgaacgattg cgcccagctt tcgacgaaat tcagatcatg ttagatgaga tgaatgattt
    10441 tcgtctgaca ccaacaggaa cgctcaaaat caatgccgcc cgggtagctg cacgtatatt
    10501 tttaatgcct ttactggttg gtttcacacg tgaatatcct gatattaaag tcgagctcac
    10561 aactgacgac tctcttgtcg atatcgtcca acagggcttt gacgctggcg ttcgacttag
    10621 tggcatcgtc gaaaaagata tgatcagcgt ggcgatcggc ccaccagtga aattatgtgt
    10681 agcagcaacg ccagagtact ttgcaagata tggcaaaccc cgccatcctc atgatctgct
    10741 taatcatcaa tgtgttgtat ttcgttatcc cagcggcaaa cctttccact ggcaatttgc
    10801 aaaggagcag gaaattgctg tcgcagggaa tatcattctg g
//
LOCUS       DAZPJR010000145        10716 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPJR010000145 DAZPJR010000000
VERSION     DAZPJR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.147"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..1093
                     /gene="dacC"
                     /locus_tag="ACHVB7_003374"
                     /pseudo
     CDS             1..1093
                     /gene="dacC"
                     /locus_tag="ACHVB7_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1140..1898)
                     /gene="deoR"
                     /locus_tag="ACHVB7_003375"
     CDS             complement(1140..1898)
                     /gene="deoR"
                     /locus_tag="ACHVB7_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR1915273.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1956..2552)
                     /gene="ybjG"
                     /locus_tag="ACHVB7_003376"
     CDS             complement(1956..2552)
                     /gene="ybjG"
                     /locus_tag="ACHVB7_003376"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1915274.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2837..4064
                     /gene="mdfA"
                     /locus_tag="ACHVB7_003377"
                     /pseudo
     CDS             2837..4064
                     /gene="mdfA"
                     /locus_tag="ACHVB7_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4105..4389)
                     /gene="ybjH"
                     /locus_tag="ACHVB7_003378"
     CDS             complement(4105..4389)
                     /gene="ybjH"
                     /locus_tag="ACHVB7_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR1915275.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4475..5290)
                     /gene="ybjI"
                     /locus_tag="ACHVB7_003379"
     CDS             complement(4475..5290)
                     /gene="ybjI"
                     /locus_tag="ACHVB7_003379"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR1915276.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5290..6498)
                     /locus_tag="ACHVB7_003380"
     CDS             complement(5290..6498)
                     /locus_tag="ACHVB7_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1915277.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6582..7118
                     /gene="rcdA"
                     /locus_tag="ACHVB7_003381"
     CDS             6582..7118
                     /gene="rcdA"
                     /locus_tag="ACHVB7_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR1915278.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7293..8978)
                     /locus_tag="ACHVB7_003382"
     CDS             complement(7293..8978)
                     /locus_tag="ACHVB7_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR1915279.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9248..9625
                     /locus_tag="ACHVB7_003383"
     CDS             9248..9625
                     /locus_tag="ACHVB7_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR1915280.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9655..9912)
                     /gene="grxA"
                     /locus_tag="ACHVB7_003384"
     CDS             complement(9655..9912)
                     /gene="grxA"
                     /locus_tag="ACHVB7_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR1915281.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10072..10359
                     /locus_tag="ACHVB7_003385"
     CDS             10072..10359
                     /locus_tag="ACHVB7_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR1915282.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10343..>10716
                     /gene="nfsA"
                     /locus_tag="ACHVB7_003386"
     CDS             10343..>10716
                     /gene="nfsA"
                     /locus_tag="ACHVB7_003386"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR1915283.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLG"
BASE COUNT         2765 a         2649 c         2714 g         2588 t
ORIGIN      
        1 gatgcgcgtg catggatttt aatggattac gccagcggta aagtgctggc agaaggcaac
       61 gcggatgaga aactggatcc cgcgagcctg actaaaatca tgaccagcta tgtggttggg
      121 caggcgctta aggccgataa gattaaactc accgatatgg tgacggtcgg taaagatgcc
      181 tgggcgacgg gaaatccggc actgcgtggt tcatcggtaa tgttcctcaa accgggcgat
      241 caggtttcgg tggcagactt gaacaaaggt gtgattatcc agtccggtaa tgacgcctgt
      301 attgcgctgg ccgattacgt tgccgggagc caggagtcat ttattggttt gatgaatggt
      361 tatgccaaaa aaactgggtc tgaccaacac taccttccag acggtgcacg gtctggatgc
      421 gccggggcag ttcagtaccg cgcgcgatat ggcattgctg ggtaaagcat tgatccacga
      481 tgtgccggaa gagtacgcca ttcataaaga gaaagagttc accttcaaca aaattcgtca
      541 gcctaaccgt aaccgtctgc tgtggagcag caatctgaat gttgatggca tgaagacagg
      601 aaccacggca ggtgcgggat ataatctggt tgcttcggct acccagggtg atatgcgttt
      661 aatctccgta gtgctggggg cgaaaactga ccgtatccgt tttaatgagt ctgagaaatt
      721 attgacctgg ggtttccgct tctttgaaac tgtgacgcca attaaacctg atgccacctt
      781 tgtgactcag cgcgtctggt ttggtgataa gagcgaagtg aatctcgggg caggcgaagc
      841 gggctccgtg accataccgc gtgggcagct gaaaaacctg aaggcgagct acacgttaac
      901 ggaaccgcag cttaccgcac cgctgaaaaa aggtcaggtt gtcgggacca ttgatttcca
      961 gcttaacggt aaatccattg agcagcgtcc gctgatcgtg atggaaaatg tggaagaggg
     1021 cggattcttt ggtcggatgt gggatttcgt gatgatgaaa ttccatcagt ggttcggcag
     1081 ctggttctct taatcttctg ataaccggat ggcgcgaaac gtcatccggt tatacgtcat
     1141 taatacatca acttaatacg gtgcgtctgc gcgtacttca catactcatc ttccgggcaa
     1201 caatcactca ccacaatatc aaagcgtttc aggtcaccca tgcgcgccgg acgcaccttg
     1261 ccaaatttac tgtggtcgac aaccagcaca tgcttttgcg ccatcgacat ggcccagtgt
     1321 tttaccggca actcttcaag attaaaacag gtagcgcctt tactgacatg cacgcccgcc
     1381 gcggaataaa aagcgatatc cgggcaaaaa ttattcaacg tttgctgaaa atcgatgggg
     1441 ttgaaaatgg cgttgctggc gtgaaattca ccaccgcaca gaaatgcgcg gcaatggggt
     1501 ttctctttca gcgccagaaa ggtatttagc gaataacaaa cggcggtaaa agggatttca
     1561 ttatcaatcg cttcaataat ccacggcgtg gtggtgccac agtcaaaaaa gagggtctga
     1621 tcgggttcta ccagtgtcgc agccagtttt gccgcccggc gtttttcttc caccaggcgg
     1681 gatttttgat cgcttaacag gtaatggctg gcactgcgcg gttccagaac aatatagccg
     1741 ccgagcaaaa cgacgggcgc actgtggttg ttcagatcgc gacgaatcgt catctccgaa
     1801 accccaagca gggcggcggc gtctttaaga tgtaacttat cgctgcgttt taattcttgc
     1861 agcagctgcc cgatacgctc ttcgcgacgt gtttccataa tccctctgaa tagttattga
     1921 agcgagccgc tcaatactac actttttagc agagatcagt cacgcaccca gcctttgcgg
     1981 atcggtaatg caaaacagac gcgataccac gattgcagac gttgatagag tttatgcccc
     2041 atcgcttgcc agataatctg ggcactaagg cagccaatca tacctgccag caatccaccg
     2101 agcatatcca gcggccagtg gacgccaaga taaacgcgcg accaggcaat gacgacggcc
     2161 agaaccatta aaagtgagcc ggaccacagg cgatgccagc ataaaaatgc cagtgcaaag
     2221 gtgaaaatca ccgtaccgtg atcgctcggg aatgagtcat ccgccgcatg atgcaggaag
     2281 ttatagccga tattttcgac aaagggtcgg tcgtgcggaa aaagatgtcc catcgtccag
     2341 gacacaaaca ggctgaccgc cagcgcgata gcgattttta tcaccagttg ccgttgtgct
     2401 gtaagccccc acaaccaaag taccgcggcc agcaacggca ccacggtaat caaatcttta
     2461 gcaataaaaa tcgccaacga gatcatccac ggagccgagt ctggcgtcgc gttaataaga
     2521 gagaatagag agagattcaa attttccagc ataacttccc gacgcaaagt gattaaaagg
     2581 gagccaatac aggcaagtcg ttgagaataa agtgcaggtt aaactgggta aagcggcatc
     2641 gtcttatttc cctcaagcgg cctgtttacg gtgggtgatt gtaacgggca taggttaaat
     2701 aaaacttaaa gaaagcgtag ctatactcgc aataatgtaa gaatgtgctt aaccgtggtt
     2761 tcagctacaa aattcgcttt ctcgttagct gcgcttttat taaactctgc gcgattatta
     2821 ttggcgaaga aattgcatgc aaaataaatt agctttcggt gccaggcttg gacgtcaggc
     2881 gttacttttc cctctctgtc tggtgcttta cgaattttca acctatatcg gcaacgatat
     2941 gatccaaccc ggtatgttgg ccgtggtgga acaatatcag gcgggcattg attgggttcc
     3001 tacttcgatg accgcgtatc tggcgggcgg gatgttttta caatggctcc tggggccgct
     3061 gtcggatcgt attggtcgcc gtccggtgat gctggcgggt gtggtgtggt ttatcgtcac
     3121 ctgtctggca atattactgg cgcaaaacat tgaacaattc accctgttgc gcttcttgca
     3181 gggcataagc ctctgtttca ttggcgctgt gggatacgcc gcaattcagg aatccttcga
     3241 agaggcggtt tgtatcaaga tcaccgcgct gatggcgaac gtggcgctga ttgctccgct
     3301 acttggtccg ctggtgggcg cggcgtggat ccatgtgctg ccctgggagg ggatgtttgt
     3361 tttgtttgcc gcattggcag cgatctcctt tttcggtctg caacgagcta tgcctgaaac
     3421 cgccacgcgt ataggcgaga aactgtcact gaaagaactc ggtcgtgact ataagctggt
     3481 gctgaagaac ggccgctttg tggcgggggc gctggcgctg ggattcgtta gcctgccatt
     3541 gctggcgtgg atcgcccagt cgccgattat catcattacc ggggagcagt tgagcagcta
     3601 tgaatatggc ttgctgcaag tgcctatttt cggggcgtta attgcgggta acttgctgtt
     3661 agcgcgtctg acctcgcgcc gcaccgtacg tgataattat gggcggctgg ccgattatga
     3721 ttggtctgtt ggtcgctgct gcggcaacgg ttatctcatc gcacgcgtat ttatggatga
     3781 ctgccgggtt aagtatttat gctttcggta ttggtctggc gaatgcggga ctggtgcgat
     3841 taaccctgtt tgccagcgat atgagtaaag gtacggtttc tgcggcgatg ggaatgctgc
     3901 aaatgctgat ctttaccgtt ggtattgaaa tcagcaaaca tgcctggctg aacgggggca
     3961 acggacagtt taatctcttc aaccttgtca acggaatttt gtggctgtcg ctgatggtta
     4021 tctttttaaa agataaacag atgggaaatt ctcacgaagg gtaaaaaaat gcctgactgc
     4081 tttgtgcgat caggcattct cgaattaatg gtgatggtcg tcaatctggt gttcgataac
     4141 catcccttca cctacgctgg caagatggcg agcataagga tgcgggcggt aagccggagc
     4201 tggcgcagga gccacataga cggtttgcgg cacagtcgcg acgctgaccg cttgtggaac
     4261 gctgactgaa tcaggaacta caaccacttt gtatccactc ggcacatcga cggtgacact
     4321 ttgcgccatc gccacaccag taaagcccat taaaagcgcg cccaacagta gacaattttt
     4381 cataatcatt tcgcctgaaa taatgcagcg ggttaatttc cgcgaattat gcagccttca
     4441 ttcaggtatt gtgttgccgg gcgtgctgcc gtttttattg gtcaaatggc gcttcgtgtt
     4501 taagcacttt atcgatcaca tccagtacgc cttcacggtt attggagcct gcccggtatt
     4561 ttgccgctgc gacgaccgcg ctgccggcat tttccattgc aaaactaaag cctgcctgac
     4621 gcagcatctc aatatcgtta ccgccatcgc caaagaccac cacttcgctg tcgtctattc
     4681 cccataattt ctgcagttgg cgaaggccat tggctttatg tacgccgggg ataatcagat
     4741 cgatgctgcc gttgccggtg tggactggca ccataatatc gccgatggcc tcatgtaatg
     4801 ctttttgtac ttgtggaatc agttcatcgg aaagattcag gccaaactta aagaaaatat
     4861 cttctaagtt gtcaaagtta tcgacgtatt ccagacggtg ataatacatt tctgccaccg
     4921 ttttcatggc atcgtcatat tttttgagcg tataggcgct attttttccg caggcaataa
     4981 tttccacttc cgggcgcgtc agcaaatgtt ccacgacagt agtaaaggca tccttcgaca
     5041 gctcgccatt aaaaacatct ttgccttcac tcactaccca gccgccgttt tcagccacaa
     5101 aggcaatttc attagcaatt tcagggaaga aagagagcaa ctgataatat tgattcccgc
     5161 tggcgaccac aaagcgaatg ccttgtgctt tcatttgctg atactgagcc ataaaccgct
     5221 cacggttata ggttttttga tcgcttaaga aagtaccatc catgtctacc gcaattaatt
     5281 taatgctcat caactattct ccatcgccgt ccgcgttttg gtatcgggtt tggcgacggc
     5341 tttcgcgaca atagccgcga gaataaccag cgccagtaca accagcattg cactacgtaa
     5401 tccataatgt tcgccgagat agcccagcag cggcggcccg acgaggaaag ccagataacc
     5461 ggtcgttgct accacactga cgcgggttgg tgcatcgggg ccggtatcgc tggcggcaga
     5521 aatggtcagc gggaagccca gcgaggcacc cagtccccag agtacaacag acaccccagc
     5581 gacccaggcg ctatcgacaa aaataatcag cccaataccc aacgccccca ttagtgcact
     5641 ggcccgaacc acggcaacgc gactgtaacg gtcgatgaac caaccgccag tgaagcgtcc
     5701 aacggtcatc cctagggtaa aaccggcata aatcagcgag ccggaagtag ggctaaaacc
     5761 gtgaccgtca accattaata agggtaacca gtcgttggca gaaccttcgg caaaggccat
     5821 cgccagcact acaacaccaa tcaacagcaa ctggatatcg cgataaaaag gaatgccttt
     5881 ttcgccatgc tgggtgccat cggcagcatt tttgcccgta ccgtcaggga ttgcctgaat
     5941 ggcgatataa ataggcgcga tacctaccag cgccgccaat aaaatgtgca ccgttgccgg
     6001 aacgccaaag gccgtcagtg ccatcccgac accagcgcct gccagcgtgc ctaggctata
     6061 aaaaccgtgc atcatcggca aaaccgtttt attcatttct cgctcaacgg cggcaccttc
     6121 aacgtttatc gccacttccg cagaaccaaa acttgcccca aagacgccga gaccaacggc
     6181 aaagagcagg ggcgatgtca gccagagtgc cagacttaat atcatcatcc cgatcaatgc
     6241 gcaggacatc gtgaccagga tgacattacg tgtcccaaag cgtttcacta accacgccga
     6301 gcagagaata ccgctcatcg aaccgatcga cagaccaaag agaacaccgc ccatttcagc
     6361 gatcgagaca gagagaatat cgcggatagc aggcgtacgg gttgcccagg acgccattaa
     6421 caggcctggc aaaaagaaga acataaacag cgcccaggtt cggcgtttca atgcattacg
     6481 tgaagaattt acggtcatag atcacgtcaa aataagaaga gggaagacaa cattagcaag
     6541 gttgtgtaca tttgtacaca attgcagaca gaggaaatga catgcgtcgc gctaacgatc
     6601 cgcaacggcg agaaaaaatt atccaggcca cgctggaggc ggtgaaactt tacggaatac
     6661 atgctgttac gcaccgcaaa attgctaccc ttgccggggt accgttggga tcgatgacct
     6721 actatttttc aggaattgat gagttgttgc tggaggcgtt cagcagtttt actgagatta
     6781 tgtcccggca atatcaggca ttttttagcg atgttagtga tgctcaaggc gcatgccagg
     6841 ctatcaccga tatgatctac agctcacagg ttgcaacgcc ggataatatg gagctgatgt
     6901 accagctcta cgcgctggct agccgaaaac cgctattaaa aacggtaatg caaaactgga
     6961 tgcagcgcag tcagcaaacg ctcgaacaat ggtttgaacc cggaaccgcc cgcgcgcttg
     7021 atgcgtttat tgaggggatg acgctgcatt ttgtcaccga ccgtaagccg ctatcgcgcg
     7081 aggagatttt gaggatggtt gagagggttg cagggtagta gataaatttt agataacaaa
     7141 aaacccatca accttgaacc gaaatggcgg ggttgatggg ctccacaaaa tggggacatc
     7201 aaagaaaagc agtggcacta attaagactg atgccctgcg gaaaagttct gcggttgtgc
     7261 aaaaaaattt cattttcagg gcaacttcag ttttatccta atcctggcca taccatgacg
     7321 atgattgtcc ctgccagcgt cagcaggacg ttggcgattg cgtaggtgcc cgcatagccc
     7381 agcgcaggga tgttactgcg agctgtatca ctgatgatct ccattgccgg cgcgcaggtg
     7441 cgtgcgccca tcattgcgcc gaacaacagt gcgcggttca ttcgcaatac ataagcaccg
     7501 aacaagaaac agataaccac gggcacaaga ctgacaatta atccggcaat caacatctga
     7561 ccgccaatcg cgcccaggcc gttattaata ccgctaccgg cgctcagacc aacgcctgcc
     7621 ataaacacca tcaagccgaa ctctttcacc atgcttaatg caccttgcgg aatgtaaccg
     7681 aaggtcgggt ggttagcacg cataaagccc agcataattc cggcgaataa caacccggca
     7741 gcgttcccca tgccgaaact gaatgtgctg aactggaagg tgatcatccc gatcatcagc
     7801 ccaataacaa agaaggcgca aaatgccagc aggtcagtga cctggctgtg aatcgagata
     7861 aagccgatgc gatcggcgat ggtttttacg cgacgggcat caccgctgac ttgtaaaacg
     7921 tcacctttgt taagcacgac gttgtcatct atcggcatct caatctggct acgaatgacg
     7981 cggttaagga agcaaccgtg atcggtcaac ttcagttgtg cgagacgttt acctacagcg
     8041 ttatggtttt taacgaccac ttcttcagtg acgatacgca tgtcgagaag gtcacgatcg
     8101 aaaacttctt taccgttacg gaagctggga tcgagtcggg catgggcgtc gggatagcct
     8161 accaacgcta tttcatcgcc catttgtagc acggcatcac cgtctggatt tgccagaatc
     8221 ccgttacgtc gaatacgttc aatgtagcag ccggtttgtc gataaatacc cagttcacgc
     8281 agatttttgc cgtcggtcca ggccaccagc tccgggccga cgcgataggc gcggatcacc
     8341 ggtaaataaa ccttacggtt ggcatcagtg tccaggccac gttcgcgggc gatttgctgg
     8401 gcgctggtct gtaagtcctg atgctgcaat ttcggcaagt aacgcgcacc aacaatcaaa
     8461 ctcaccagac cgattaaata ggttaaggca tacccgaggc tcagattatc cagtgccagt
     8521 gagagctgcc tgctttccat gccggaatga cgcagtgtat cgccagcacc gaccagaacc
     8581 ggtgtcgacg tcatagagcc tgctaacata ccggccgtca ggccaatatc ccagccaaac
     8641 agcttaccta accctaaggc gatcaccagc gcactgccaa ccatcaccag tgctaacatt
     8701 aggtaatttt tcccatcgcg aaaaaaaatg gaaaaaaagt tcggtccggc ttcgaccccg
     8761 acgcagaaaa taaacagcat aaagccaaga ttaagcgcat cggtgttaat gctgaaatgt
     8821 tgttggccta ataacagcga tacgactaaa acgccaatgg aattacccag ttggatcgaa
     8881 ccaagtcgta actttccgag acatagccca agcgtgagga ccacaaataa taacagaatg
     8941 taattcccat ttaacaattc ggcgacgttt atattcacgg aggctaactt cttgtttact
     9001 agtaagctgt tgaaagaaat ggtaatttac gataatgttt tttaccagaa ttcagggcgc
     9061 agattcattc agcgcaccta aacgatagta aagtaacaat atattttact agtgtaatca
     9121 cattaggtat caacggctat atgaattgcg ttggcctata ttagcatgga atgcgaagcg
     9181 gctttatctt actgaacgcc acactggcga aaaatgtgtt cgatagacgc agtgtcagga
     9241 ggaacgagtg aaacataaac aacgttgggc gggggcaatc tgctgttttg tcctcttcat
     9301 tgtggtgtgc ctttttctgg cgacgcacat gaaaggcgct tttcgggctg ccgggcatcc
     9361 tgaaatcggc ttgctgtttt tcattcttcc tggagcagtt gccagtttct tttcacagcg
     9421 tagagaagtc ctgaaacctc tatttggcgc aatgctggcg gcaccctgtt caatgctcat
     9481 tatgcggctg tttttttcac cgacgcgctc attctggcaa gagctggcat ggttactaag
     9541 cgcggtgttc tggtgtgcgc tgggggcact gtgtttctta tttatcagta gtttgtttaa
     9601 accacagcac agaaaaaatc agtaaagccc tcaacgcgag ggcttgtcag acgatcaggc
     9661 gtccagattt tctttcaccc atgcagcaaa atcggtatag ccgccgatat gttgctgatc
     9721 gacaaaaatc tgcggcacgg tttctacggg tttacctgcc ttttgttgta gatcttcttt
     9781 agtgatccct tccgcacgaa tatctacata ctgatactga aaatcatcgc gttcattgct
     9841 caatttctca gccagatctt ttgcacgcac acagtaaggg caacccgaac gaccaaaaat
     9901 aacggtttgc attatttctc tcctcataga tttatgcctg taatgatcac gctaaaatgt
     9961 attcgctgaa agtaggttta acctgttgca ttaattgcta aaagctataa ctgttaaaca
    10021 caatacagtg aaaagtttta gactgaaggc tcactttgca gagggaagcg tatgcgcgcg
    10081 atcggtaaat tgcctaaagg cgtgttgata ctggaattta tcggaatgat gctactggcg
    10141 gtggcgctgc tgtcggtaag cgactccctg tcgctgcctg agccattttc tcggccagaa
    10201 gtgcagattc tgatgatttt tctcggtgtt ttgctcatgc ttcccgctgc ggtggtggtt
    10261 attcttcagg tggcaaaacg tcttgcccca cagctgatga accgtccacc gcaatattca
    10321 cgttcagaaa gagaaaaaga taatgacgcc aaccattgaa cttatttgtg gccatcgctc
    10381 cattcgccat ttcactgatg aacccatttc cgaagcgcag tgtgaggcga ttattaacag
    10441 cgcccgtgcg acgtccagtt ccagtttttt gcagtgtagt agcattattc gcattaccga
    10501 caaagcatta cgtgaagaac tggtgacgct gaccggcggg caaaaacacg tagcgcaagc
    10561 ggcggagttc tgggtgttct gtgccgactt taaccgccat ttacagatct gtccggatgc
    10621 tcagctcggc ctggcggaac aactgttgct cggtgtcgtt gatacggcaa tgatggcgca
    10681 gaatgcatta accgcagcgg aatcgctggg attggg
//
LOCUS       DAZPJR010000146        10686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPJR010000146 DAZPJR010000000
VERSION     DAZPJR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.148"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHVB7_003387"
     CDS             complement(<1..784)
                     /locus_tag="ACHVB7_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR1915284.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHVB7_003388"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHVB7_003388"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR1915285.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHVB7_003389"
     CDS             1860..2747
                     /locus_tag="ACHVB7_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR1915286.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHVB7_003390"
     CDS             2780..3790
                     /locus_tag="ACHVB7_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR1915287.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHVB7_003391"
     CDS             3860..5293
                     /locus_tag="ACHVB7_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1915288.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHVB7_003392"
     CDS             5392..6741
                     /locus_tag="ACHVB7_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1915289.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHVB7_003393"
     CDS             6731..7618
                     /locus_tag="ACHVB7_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1915290.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHVB7_003394"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHVB7_003394"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR1915291.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHVB7_003395"
     CDS             8352..9224
                     /locus_tag="ACHVB7_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR1915292.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHVB7_003396"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHVB7_003396"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR1915293.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHVB7_003397"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHVB7_003397"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR1915294.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2461 a         2532 c         2855 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccggg taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAZPJR010000147        10648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPJR010000147 DAZPJR010000000
VERSION     DAZPJR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.149"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHVB7_003398"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHVB7_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR1915295.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="ACHVB7_003399"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="ACHVB7_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR1915296.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="ACHVB7_003400"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="ACHVB7_003400"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR1915297.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="ACHVB7_003401"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="ACHVB7_003401"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR1915298.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="ACHVB7_003402"
     CDS             complement(5254..6294)
                     /locus_tag="ACHVB7_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1915299.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="ACHVB7_003403"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="ACHVB7_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR1915300.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHVB7_003404"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHVB7_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR1915301.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="ACHVB7_003405"
     CDS             7229..7669
                     /locus_tag="ACHVB7_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR1915302.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHVB7_003406"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHVB7_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR1915303.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHVB7_003407"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHVB7_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR1915304.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..>10648
                     /gene="rsxC"
                     /locus_tag="ACHVB7_003408"
     CDS             8898..>10648
                     /gene="rsxC"
                     /locus_tag="ACHVB7_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR1915305.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQV"
BASE COUNT         2450 a         2751 c         2720 g         2727 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggt
//
LOCUS       DAZPJR010000148        10454 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPJR010000148 DAZPJR010000000
VERSION     DAZPJR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.150"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            133..708
                     /gene="ssuE"
                     /locus_tag="ACHVB7_003409"
     CDS             133..708
                     /gene="ssuE"
                     /locus_tag="ACHVB7_003409"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR1915306.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            701..1660
                     /gene="ssuA"
                     /locus_tag="ACHVB7_003410"
     CDS             701..1660
                     /gene="ssuA"
                     /locus_tag="ACHVB7_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR1915307.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1657..2804
                     /gene="ssuD"
                     /locus_tag="ACHVB7_003411"
                     /pseudo
     CDS             1657..2804
                     /gene="ssuD"
                     /locus_tag="ACHVB7_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2816..3607
                     /gene="ssuC"
                     /locus_tag="ACHVB7_003412"
     CDS             2816..3607
                     /gene="ssuC"
                     /locus_tag="ACHVB7_003412"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR1915308.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3604..4371
                     /gene="ssuB"
                     /locus_tag="ACHVB7_003413"
     CDS             3604..4371
                     /gene="ssuB"
                     /locus_tag="ACHVB7_003413"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1915309.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4578..7190)
                     /gene="pepN"
                     /locus_tag="ACHVB7_003414"
     CDS             complement(4578..7190)
                     /gene="pepN"
                     /locus_tag="ACHVB7_003414"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR1915310.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7456..8658
                     /gene="pncB"
                     /locus_tag="ACHVB7_003415"
     CDS             7456..8658
                     /gene="pncB"
                     /locus_tag="ACHVB7_003415"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR1915311.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8831..10231
                     /gene="asnS"
                     /locus_tag="ACHVB7_003416"
     CDS             8831..10231
                     /gene="asnS"
                     /locus_tag="ACHVB7_003416"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR1915312.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2350 a         2696 c         2819 g         2589 t
ORIGIN      
        1 gccctttctt tagtttattt tcagaaaaag atacacaaaa tttatatttg gcattttctt
       61 gtctctccga cagactgacc gaattgttat caatgttaac aaaaaaagaa caattggtta
      121 taaggagaga gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc ttctcgctcc
      181 agctccttgc tggaatatgc gcgggaaaaa ctaaatggcc tggatgtaga ggtttatcac
      241 tggaatctgc aaaacttcgc cccggaagat ctgctttacg ctcgtttcga tagtccggca
      301 ctcaagacct tcaccgaaca gctgcaacag gctgatggac tgatagtcgc tacacctgtg
      361 tataaagccg cctattccgg cgcgctgaaa accctgctcg acctgctgcc agagcgcgct
      421 ttgcaaggca aagtggtgct accgctggcg acgggcggta cggtggctca tctgctggcg
      481 gtcgattatg cccttaagcc agttttaagt gcgatgaaag cccaggagct cctgcacggc
      541 gtgtttgccg atgactcaca agttattgat taccatcaca aaccccagtt cacgccaaat
      601 ctgcaaaccc gtcttgatac cgcgctagaa actttctggc aggcattgca ccgccgcgat
      661 gttcaggttc ctgaccttct gtctctgcga ggtaatgtcc atgcgtaaca tcattaaact
      721 ggcgctggcg ggattgctta gcgtctctac ttttgcggtt gctgcagaat cctcgcctga
      781 agcgttacgt ataggctatc agaaaggcag tattggtatg gtattggcaa aaagccacca
      841 gttactggaa aaacgctatc cgcaatcaaa aatctcctgg gtggagttcc ccgcgggtcc
      901 gcaaatgttg gaagcgttaa acgttggcag tattgatctc ggcagtaccg gggatattcc
      961 gccaatcttt gcccaggctg ccggggctga tttgatatat gtgggcgtcg agccaccgaa
     1021 gcccaaagcc gaagtgattc tggtggcaga aaacagcccg atcaaaaccg tagccgatct
     1081 taaaggtcac aaagttgcct ttcagaaagg ttccagttca cacaaccttt tactgcgtgc
     1141 actgcgtcag gccggactta agtttactga catccagccc acttatctga cgccagctga
     1201 tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg atccctacta
     1261 ctccgctgcg ttattacagg gcggcgtgcg ggtgctgaaa gacggcaccg atctcaatca
     1321 aactggatcg ttttatctgg cagctcgccc ctatgcagaa aaaaacggcg cttttattca
     1381 gggcgtactg gcaaccttta gtgaggccga tgcgttaacc cgcagccagc gcgagcaaag
     1441 catcgcttta ctggcaaaaa cgatgggctt accggcaccg gtgattgcct cgtatctgga
     1501 tcatcgtcct cccaccacca tcaaaccggt gagcgctgaa gttgccgcct tacagcagca
     1561 aacggcagat ctgttttatg aaaaccgtct ggtgtcgaaa aaagttgata ttcgccagcg
     1621 catctggcag cccactcacc tggaaggaaa acaattatga gtctgaatat gttctggttt
     1681 ttaccgaccc atggtgacgg gcattatctt ggaacggaag aaggttcacg cccggttgat
     1741 cacggctatc tgcaacaaat tgcgcaagcg gcggatcgtc tgggctatac cggtgtgctg
     1801 atcccaacgg ggcgatcgtg tgaagatgcg tggctggtgg cggcgtcgat gatcccggtg
     1861 acgcagcggc tgaagtttct tgtcgccctg cgtcccagcg taacctcacc taccgttgcc
     1921 gctcgccagg ccgccacgct tgaccgtctc tcaaatggtc gtgcgttgtt taatctggtc
     1981 acaggcagcg atccacaaga gttggcaggc gacggcgtgt tccttgatca tagcgagcgc
     2041 tacgaagcct cggcggaatt tacccaggtc tggcggggcg cttattgctg ggggaaagcg
     2101 tcgatttcaa cggtaaacat attcatgtgc ggggagcaaa actgctcttc ccgccgattc
     2161 aacagccgta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2221 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc agagctggta aaagagaaga
     2281 tcgaacaagt gcgggcgaaa gctgccgcgc atggacgcaa aattcgtttc ggtattcgtc
     2341 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2401 cgcatcttga tgatgaaact atcgccaaag cacaggccgc attcgcccgg acggattccg
     2461 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2521 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2581 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2641 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2701 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2761 cggtggcgaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2821 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2881 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2941 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaattgt ggcagcatct
     3001 ggcgatcagt tcctggcggg cgctgattgg cttttcaatt ggcggatcgc tgggcttgat
     3061 tctgggactg attagcgggc tgtcacgctg gggagaacgg ctgctggata cctcaattca
     3121 gatgttgcgc aacgtaccgc atctggcgct gatcccgctg gtgattttgt ggtttggcat
     3181 tgatgaatcc gcaaaaatct ttctggtggc gctcggcacg ctattcccca tttatatcaa
     3241 cacctggcat ggcatccgta atatcgatcg cggtctggtg gaaatggcgc gtagctatgg
     3301 attatccggc ataccgctgt ttatccatgt gatcctgcct ggtgccctgc cctcaattat
     3361 ggtcggcgtg cgttttgcgt tgggcctgat gtggctgacg ctgattgttg ccgaaaccat
     3421 ttctgccaat tcaggcattg gttatctggc gatgaatgcg cgggagtttt tgcaaacgga
     3481 cgtggtggta gtcgctatta ttctttacgc cctgctcggc aaacttgctg acgtcagcgc
     3541 gcagctgtta gagcgcctct ggttgcgctg gaacccggct tatcatttga aggaggccac
     3601 ggtatgaata ctgctcgtct gaaccagggc acaccattgt tgctcaatgc agtaagcaaa
     3661 cattacgcgg aaaatatcgt cctgaaccaa ctggatttac atattccggc aggtcagttt
     3721 gtggcggtgg tgggccgcag cggtggtggc aaaagtaccc tgctgcgcct gctggcaggt
     3781 ctggaaacgc caaccgcagg cgatgtgtta gcgggcacca caccgctggc tgagattcag
     3841 gaagacacgc gaatgatgtt tcaggatgcg cgtctgctgc cgtggaaatc ggtgattgat
     3901 aacgttgggt taggccttaa aggccagtgg cgcgatgccg cgcgtcgggc gttggctgca
     3961 gtaggactgg agaatcgcgc cggggaatgg cctgccgcac tttctggcgg gcagaaacag
     4021 cgtgtggcgc tggcaagagc gttgattcat cgaccgggtt tattgttgct tgatgaaccg
     4081 ctcggggcac tggacgcctt aacgcgactc gagatgcagg atttgattgt gtcactttgg
     4141 cagcagcacg gctttaccgt actgttggtg acgcatgatg tgagcgaagc ggtagcaatg
     4201 gctgaccggg tgttgttaat tgaagaggga aaaattggtc tggatttgac agtggatatc
     4261 ccccgtccgc gtcgattagg gtcggtgcga ttagcggagc tggaagcgga agtattgcaa
     4321 cgggtaatgc ggcgtgggca ttctgaacaa ctgattcggc gtcatggtta atatgccgga
     4381 tgtggcgcac aaaaccagca ttttcaacgg acttgtaggc ctgataagac acgtcaagca
     4441 tcgcatcagg cagttgggca cggctgccgg atgcggcgtg aacgccttat ccagcctaca
     4501 aaaccagcaa tttcaacgga gttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
     4561 attttggctc aaaagtatca agccagtgct ttggtgatct tctcgtacag atcgccagag
     4621 agattttcca gccctttcaa ctgttccagc gccgcgcgca ttttctcctg acgtttggca
     4681 tcataacgtt tcaggcgaat cagcggttca atcagacgtg aagctacctg cgggttacgg
     4741 ctgttgaggt cggtaagcat ttcctccagg aactggtaac cgctgccatc ttcggcatgg
     4801 aacgctgccg ggttgctgcc cgcaaacgca ccaatcaacg aacgaatacg gttcgggttg
     4861 ctcatggtaa atgagcgatg ctgcaacaga ccacgcaccg tctccagcac attcgccgcc
     4921 gggctggtgg cttgcaggat aaaccattta tccatcacca gaccgtcctg atgccactta
     4981 tcgtcgtact cctgcatcag cgcgtcacgg caaggcagct gtgcagcaac cgccgcagaa
     5041 agcgctgcca gcgcatcggt catattgttt gcttcatggt attgcttgct caccagcacg
     5101 tcagccagat gcgtttcacc aaaagcaagg aagcgcaggc aggcatttcg cagagtgcgt
     5161 tttgcaatat cttcatgctc aacacggtac tcgctctggt aattcgcgtt gtaaatagcc
     5221 agcagctcat ccgccagttc agtcgccaga gtacgagtga gtgcttcgcg tacttcggca
     5281 atagcaatcg ggtcgatgat atcgaacagt tcagccattt cattgacaga aggcagcgtc
     5341 aggatttctg ccgccagcgc cggatcaatc ttctcatcga gcagcaccgc gcggaaagcg
     5401 tcagccacat gtaccggcag agacagcggc tgcccttgct gatgacgggc gacgttcagc
     5461 ttgatgtagg ttgccagcaa actttgcgcc gcatcccaac gggagaaatc attacgcgca
     5521 tgacgcatca ggaaggtcag ttgctgatca ctccacttat attccagttt cactggcgca
     5581 gagaattcgc acagcagcgc aggcaccggc tggaagtaga cattatcaaa aacaaaggtc
     5641 tgttccgcct gggtgacgtt cagcacggaa ttcaccggat gaccgccttt ctgcaacggg
     5701 atcactttgc cttcgttgtc atacaattcg atggcaaacg gaatatgcag cggctgtttt
     5761 tctgcctgat ccggcgtggc tggcgtgcgc tggctgatgg tcagggtgta ctgctcagtt
     5821 tccggattgt agtcgtcttt gacggtcaca atcggcgtac cggactggct gtaccaacgg
     5881 cggaaatggg agagatcaac attcgacgca tcttccatcg cctgcacaaa gtcgtcacag
     5941 gtcgctgcgc taccatcatg acgctcgaaa tagagttgca tccctttctg gaagttttct
     6001 tcgcccagca gggtgtggat catacggatc acttccgcgc ccttctcgta aacggtcagg
     6061 gtgtagaagt tgttcatctc aatgaccatg tccgggcgga ttgggtgcgc catcgggctg
     6121 gcgtcttccg caaactgcaa tccgcgcatg gtgcgcacat tattgatgcg gttaactgcg
     6181 cgggaaccaa ggtcagagct gaattcctga tcgcggaaga cggttaaacc ttctttcagg
     6241 ctgagctgga accagtcgcg gcaggtcact cggttaccgg tccagttatg gaaatattca
     6301 tggccgataa cgcgttcaat atcgaggtaa tctttgtcgg tggcggtgtc ggtgcgggcc
     6361 agcacatatt tggagttaaa gatattcaga cccttattct ccattgcgcc catattgaag
     6421 aaatccaccg cgacgatcat atagatgtcg aggtcatact ccaggccgaa gcgttcttca
     6481 tcccatttca ttgagttttt cagcgaggtc atcgcccacg gcgcgcgatc aaggttgccg
     6541 cgatcgacgt acagctccag cgccacttcg cgaccagaac gcgtggtaaa ggtgtcgcgc
     6601 agtacatcaa agtcgcctgc caccagcgca aacaggtagc acggtttcgg gaacgggtcc
     6661 tgccactgta cccaatggcg tccgttttcc agttcacctt gcgcaacgcg gttaccgttg
     6721 gaaagcagga agggatattt ggttttatcg gcaataattt tggtggtaaa acgcgccagc
     6781 acgtccgggc ggtcgagata atacgtaata tggcggaaac cttcggcttc acactgggtg
     6841 caaagcgcat cgcctgactg ataaagccct tccagcgcgg tattcgccgc cgggcttatt
     6901 tcattaacga tcttaagcgt aaaatgctcc ggcaaattac tgatgaccag tgcgccctct
     6961 tcttctttcc aggcggtcca cggctcatca ttaatatgaa cagaaaccag tttgaggtct
     7021 tcgccgttga gacgaagtgg agcatctgat gcaccatgac ggacagcctg gctgaccgcg
     7081 gtaacgaccg tcttttgcgc gtcgaggtca aaggtcaagt caatatcagt aatctggtaa
     7141 tctggcgcac gataatcgtg acggtatttg gcttgtggct gttgagtcat aaataacctt
     7201 tagcatcttt tatagagtct ggtgttcagt ctattcctgt tgcgtaaatc gcgctatgca
     7261 gaatcttcat cttttcaggt acaaacgcct ttattgctac atttttataa catacaccgc
     7321 gtaatgccat cgaccagaaa ggtggcatat ggtgtgatcg gggttcaata aattgcgaaa
     7381 caaggtatac tccagcagtt cctgaagatg tttattgtac taaacgctcc tgtacgagga
     7441 cgctactgcg cacctatgac acaattcgct tctcctgttc tgcactcgtt gctggataca
     7501 gatgcttata agttgcatat gcagcaagcc gtgtttcatc actattacga tgtgcatgtc
     7561 gcggcggagt ttcgttgccg aggtgacgat ctgctgggta tttatgccga tgctattcgt
     7621 gaacagattc aggcgatgca gcacctgcgc ctgcaggatg atgaatatca gtggctctct
     7681 gccctgcctt tctttaaggc cgactatctt aactggttac gcgagttccg ctttaacccg
     7741 gaacaagtca ccgtgtccaa cgataatggc aagctggata ttcgcttaag cggtccgtgg
     7801 cgtgaagtca tcctctggga agttcctttg ctggcggtta tcagtgaaat ggtacatcgc
     7861 tatcgctcac cgcaggccga cgttgcgcaa gccctcgaca cgctggaaag caaattagtc
     7921 gacttctcgg cgttaaccgc cggtcttgat atgtcgcgct tccatctgat ggattttggc
     7981 acccgtcgcc gtttttctcg cgaagtacaa gaaaccatcg ttaagcgtct gcaacaggaa
     8041 tcctggttcg tgggcaccag caactacgat ctggcgcgtc ggctttccct cacgccgatg
     8101 ggaacacagg cacacgaatg gttccaggca catcagcaaa tcagcccgga tctagccaac
     8161 agccagcgag ctgcacttgc tgcctggctg gaagagtatc ccgaccaact tggcattgca
     8221 ttaaccgact gcatcactat ggatgctttc ctgcgtgatt tcggtgtcga gttcgctagt
     8281 cggtatcagg gcctgcgtca tgactctggc gacccggttg aatggggtga aaaagccatt
     8341 gcacattatg aaaagctggg aattgatcca cagagtaaaa cgctggtttt ctctgacaat
     8401 ctggatttac gcaaagcggt tgagctatac cgccacttct cttcccgcgt gcaattaagt
     8461 tttggtattg ggactcgcct gacctgcgat atcccccagg taaaacccct gaatattgtg
     8521 attaagttgg tagagtgtaa cggtaaaccg gtggcgaaac tttctgacag ccctggcaaa
     8581 actatctgcc atgataaagc gtttgttcgg gcgctgcgca aagcgttcga ccttccgcat
     8641 attaaaaaag ccagttaata tcatcaggga gctaatcggc tccctttttt tacctttaat
     8701 tccgaaatct ttcgctgcat ttgcgaattc tgcttgtctg attgcagatg ccaggtaaca
     8761 taggtatccc cccccccatt tacgggtgga caagtgttta ttttttccga ctattaacag
     8821 agagaatatt atgagcgttg tgcctgtagc cgacgtactc cagggccgcg tagccgttga
     8881 cagcgaagtc accgtacgcg gatgggtacg tacccgccga gattcaaaag ctggcatctc
     8941 cttcctcgcc gtttatgacg gttcctgctt tgatcctgta caggctgtca tcaataattc
     9001 tctgcccaat tacaatgaag acgtcctgcg tctgaccacc ggctgctcgg tcattgtgac
     9061 gggtaaagtc gtggcatcgc cgggccaggg gcaacaattt gaaattcagg ccagcaaggt
     9121 tgaagttgct ggttgggttg aagatccaga cacttacccg atggcggcaa aacgccacag
     9181 cattgagtat ctgcgtgaag tcgctcacct gcgtccgcgc acaaacctga ttggtgccgt
     9241 cgcgcgcgtt cgccatacgc tggcgcaggc gctgcatcgc ttctttaacg agcagggatt
     9301 cttctgggtt tcaacgccac tgattaccgc atctgatacc gaaggtgcag gcgaaatgtt
     9361 ccgcgtttct acgctggatc tggaaaacct gccgcgtaac gatcagggca aagtggattt
     9421 cgacaaagac ttctttggta aagagtcttt cctgaccgta tctggccagt tgaacggcga
     9481 aacctacgct tgcgcattat ccaaaattta taccttcggc ccgactttcc gtgctgaaaa
     9541 ctccaacacc agccgtcacc tggcggaatt ctggatgctg gagccggaag tggcgtttgc
     9601 taacctgaac gatatcgcgg gtctggctga agccatgctg aaatatgtct tcaaagcggt
     9661 tctcgaagaa cgcgcagacg acatgaaatt cttcgctgaa cgcgtagata aagatgccgt
     9721 ttcacgtctg gaacgcttca ttgaggccga tttcgcgcag gtggattaca ccgacgcagt
     9781 gaccattctc gaaaactgcg gcaggaagtt tgaaaacccg gtttactggg gcgtcgatct
     9841 ctcttctgag catgagcgtt atctggcgga agaacacttt aaagcaccgg tagtggttaa
     9901 aaactatccg aaagatatta aagcgttcta tatgcgcctc aacgaagacg gtaaaaccgt
     9961 tgcggctatg gacgttctgg ctccgggcat cggtgagatc attggtggct cccagcgtga
    10021 agaacgtctg gacgttctgg acgagcgtat gctggaaatg ggcctgaata aagaagatta
    10081 ctggtggtat cgcgatctgc gtcgctacgg tactgttccg cattcaggtt tcggtcttgg
    10141 ttttgaacgc ctgattgctt acgtaactgg cgtgcaaaac gtgcgtgatg tgattccgtt
    10201 cccacgtact ccgcgcaacg ccagcttctg attttcttcc tcaaagagcc agcgcctgct
    10261 ggctcttttt tatcctttct gtcaagttat ctgtttgtta agtcaagcaa tctatttgca
    10321 accccgccat aacgatcatc ctgttacgga atattacatt gcaacattta cgcacaaaaa
    10381 ataacccgca ttcttattgc ggattagttt tttcttagct gatagcacaa ttttcatact
    10441 attttttggc attc
//
LOCUS       DAZPJR010000149        10447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPJR010000149 DAZPJR010000000
VERSION     DAZPJR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.151"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..190
                     /gene="bdm"
                     /locus_tag="ACHVB7_003417"
     CDS             <1..190
                     /gene="bdm"
                     /locus_tag="ACHVB7_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR1915313.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            292..429
                     /gene="sra"
                     /locus_tag="ACHVB7_003418"
     CDS             292..429
                     /gene="sra"
                     /locus_tag="ACHVB7_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR1915314.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            586..2283
                     /gene="maeA"
                     /locus_tag="ACHVB7_003419"
     CDS             586..2283
                     /gene="maeA"
                     /locus_tag="ACHVB7_003419"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1915315.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDIGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2417..3419
                     /gene="adhP"
                     /locus_tag="ACHVB7_003420"
                     /pseudo
     CDS             2417..3419
                     /gene="adhP"
                     /locus_tag="ACHVB7_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3565..3849
                     /locus_tag="ACHVB7_003421"
     CDS             3565..3849
                     /locus_tag="ACHVB7_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1915316.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHVB7_003422"
     CDS             complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHVB7_003422"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR1915317.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHVB7_003423"
     CDS             complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHVB7_003423"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR1915318.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHVB7_003424"
     CDS             complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHVB7_003424"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8080..8082),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1915319.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8899..9780
                     /gene="yddG"
                     /locus_tag="ACHVB7_003425"
     CDS             8899..9780
                     /gene="yddG"
                     /locus_tag="ACHVB7_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR1915320.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10039..>10447
                     /locus_tag="ACHVB7_003426"
     CDS             10039..>10447
                     /locus_tag="ACHVB7_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1915321.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGD"
BASE COUNT         2561 a         2690 c         2623 g         2573 t
ORIGIN      
        1 ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcaagg
       61 tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa
      121 cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc
      181 tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct
      241 tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg
      301 aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata
      361 gttaccgaag gtgacaaatc cagcgtggta aataacccaa ccggcagaaa acgccccgct
      421 gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg
      481 gtgtttttgt ttttatctgc tttatacttg gggccgacgc cctggcggta aagcaaagac
      541 gataaaagcg tgccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca
      601 aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg
      661 ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta
      721 ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag
      781 ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga caccaacgaa
      841 accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc
      901 ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg
      961 tttatctctt accagaaccg ccacaatatg gacgatattc tgcaaaacgt accgaaccat
     1021 aatatcaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg tgaccagggc
     1081 atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc
     1141 agcccggcgt atacccttcc ggtggtgctg gatatcggaa cgaacaacca acaactgctt
     1201 aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa
     1261 ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cagacgtgct gttgcagttt
     1321 gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt
     1381 tcttttaacg atgacattca gggcactgcg gcggtaacag tcggcacact gatcgcagca
     1441 agccgagcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca
     1501 gcgggatgcg gcattgccga aatgatcatc gcccagaccc agcgcgaagg attaagcgag
     1561 gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg gcctgctgac ggacaagatg
     1621 ccgaacctgc tgcctttcca gaccaaactg gtgcagaaac gcgaaaacct cagtgactgg
     1681 gataccgaca gcgatgtgct gtcactgctg gatgtggtgc gcaatgtaaa accagatatt
     1741 ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcatccg tgagatgcat
     1801 aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg cgtggaagcc
     1861 acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac gggcagcccg
     1921 tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt
     1981 attttcccgg gcatcggcct gggggttatt gcttccggcg cgtcacgtat caccgatgag
     2041 atgctgatgt cggcaagtga aacgcttgca cagtattcgc cgctggtgct gaacggcgaa
     2101 ggtctggtac taccggaact aaaagatatt cagaaagtct cccgtgcaat tgcgtttgcg
     2161 gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cctctgccga agctttgcaa
     2221 caagccattg acgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc
     2281 taagcctgcg cccggtagtg aaggctaccg ggcaattttc ctctcccttt ttcagatctc
     2341 atccatactg ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat
     2401 ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcatg ttgacgttac
     2461 ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg
     2521 tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa ccgacgtaat
     2581 tctgggccat gaaggcatcg gtgtggtggc agaagtgggt ccaggtgtca cctcattaaa
     2641 accaggcgat cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt gcgaatactg
     2701 taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg
     2761 cggaagagtg catcgtggtc gccgattacg cggtaaaagt gccagatggt ctggactcgg
     2821 cggcggccag cagcattacc tgtgcggggg tcaccaccta caaagccgtt aagctgtcaa
     2881 aaattcgtcc cgggcagtgg attgctatct acggtcttgg cggtctgggt aacctcgccc
     2941 tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc cattgatgtc aatgatgagc
     3001 agttaaaact ggcaaccgaa atgggtgcag atttagcgat taactcacgc accgaagacg
     3061 ccgccaaaat tgtgcaggag aaaaccggtg gcgctcactc tgcggtggta acagcagtag
     3121 ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg
     3181 tcggtctgcc gccggagtct atgagcctgg atatcccacg tcttgtgctg gatggcattg
     3241 aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac cgaagccttc cagtttgccg
     3301 ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt agcggacatc aacaccatct
     3361 ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt gattgatttc cgtcactaag
     3421 aggtctttgc tgcgactccc atgttcgggt cgcagcatcg cacactctcc aacatgaaat
     3481 ggctaaatgg attattgttg cgtaggaagg aacaataatg atagtgttcc cagagaagga
     3541 acgctactga gagtaaagac actaatgaaa atggccaatc atccccgccc gggggacatt
     3601 attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa
     3661 attgcgccct caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg
     3721 gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
     3781 gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgattacg ccgtctggtt
     3841 acgcaataaa aaatggcacc ttataaagcg ccatttgaaa ctgatgacaa acgcaacatt
     3901 gcctgatgcg ctacgcttat caggcctaca cgttcacttc aatctattgt atttgaaggg
     3961 ttttcatgcc ggataaggca ttcacgacgc atccgggatg aacaaagccc acgttgtcta
     4021 caatatgaaa atggcgcctt ttacagcgcc attttcagct taaagttcaa aagagattat
     4081 atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata ccagcgcgga
     4141 tggtgtttct tcgcccagcg acggctcacc ttcccttcga tcatcccttt aatcgaccct
     4201 ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat gatacccgca
     4261 gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg ggaagtactg cgcaaagtac
     4321 ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag
     4381 aacatcatct tttgcccggc gttgtactta ccaacatccg ccactttgtg ctcattgcct
     4441 tttaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg gatgttgtga
     4501 tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa tcgcaatgcc gaagaacggg
     4561 tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg
     4621 aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac ggtccagtga
     4681 caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat gatgatcctc
     4741 ctcgtcatcg tccacttcct tattcgggcc aataccgatg tagtggaaaa tcaacccggc
     4801 aaaagtggca ataaagccag ccgctgccag cggtttcaac gcgcctttcc acaggcttac
     4861 cgaggtgtcg atcttcggat ctttcggcag accgtaatac agctccggct gatcggcgtg
     4921 atgcagcacg tacataacgt gcgtaccacc gacccctttc gggttgtaga cgccagcatg
     4981 ttcgtaacca cgcgctttca gtttcgccac gcgctcttcc gccagctcca gcatctcctt
     5041 cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg gttcctggcc
     5101 gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct ctttgttgag
     5161 gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc agttttccga
     5221 ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg ggcacgcctt
     5281 caggcagccg ggatcttcac agtgcataca gccgtcttta cggatcagcc actccagctt
     5341 gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc tcagatcggc
     5401 ggggttatcg taaaccccga cgcagtgccc cacttcatca cggatgtcgt tccactccga
     5461 acacgccacc tgacaggctt tacagccgat acaggtggaa acgtcgataa gttttgcgac
     5521 ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg cggacctttt
     5581 gataatatcc tgcgtttcca tagccatctg ttcgccccct tacgccttct cgatgttgac
     5641 taagaacgct ttatattccg gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt
     5701 gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggattg gaatacccac
     5761 cgtttcaacc tgctgaccgt ttacattcag cggtttcaga cggcgcgtta ccaccgccac
     5821 cgcgcggata aagccacgct tgcttgagac agtgacacga tcgccattat taatgccttt
     5881 cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcaa ttgcgttgag
     5941 caatgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg tacccacata
     6001 cgggaactgc tcttttttac ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg
     6061 gttagacacc acgttcggat gcagcgggtt agtgcccagc ggcgtttcaa tcggctcgta
     6121 gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc ccatcccttc
     6181 cggctgcatg ataaacggcc cggttggcgt acccggtgcg gcattgccga agtccggaat
     6241 atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg gatcccacgg
     6301 tttaccattg atatccgccg aagcacggtt gtagagcacg cgacggttga gcggccacgc
     6361 ccaggcccat cccagcgtat tccccagacc ggatgggtcg gagttatcgc ggttagccat
     6421 ctggttgccc tgctctgtcc agctaccggt gtagatccag caagaagatg cggttgtacc
     6481 gtcatcacgc agatgcgcaa agctactcag caactgacct ttcttcgcaa tcagcacgcc
     6541 attagcgtca tagagatctt ccagcgcata gccgttattc tctttcgcca cttcgtcaga
     6601 ttgcggttcg tgcggctgct tgtagttcca gctcatcttc atcagcggtt ctacgccttt
     6661 accaccttcg gcctggtaca gctcgcgcag atgatggtag atacccgcca gaatttcgcc
     6721 gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca accagcgacc
     6781 ggagttagcg atagaaccat cttcttcggc aaagcaggtc gaaggcagac ggaatacttc
     6841 agtctgaata gacgccggat cgacatcgtt cgactcgccg tggttctgcc agaaggtaga
     6901 ggtttcagtc accagcggat cgataaccac catgtacttc agcttgctca ggcagctcac
     6961 cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga agtaaccggt
     7021 gactttgcct tcgtccatca tgttgaaata cttgatgacg tcgtaggtct ggtcccactt
     7081 cggcagccag tcatagcccc agttgttctc tttctgcgcg gcatcgccat agaaagattt
     7141 catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat cagccagcgt
     7201 cgctttcggc gtgttcgctt ccagatacga ctgcaaatca acctgttttt ctgacggaag
     7261 cgtcagataa cctggcaggc tggtagagag cagacctaag tcagtcagcc cctgaatgtt
     7321 ggagtgacca cgcaatgcgt tcacgccgcc accggccata cccatgttac cgagcagcaa
     7381 ctggatcatc gccatagtac ggatgttctg cgcacccacg gtgtgctgcg tccagcccag
     7441 cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg gaggccagca cttcacacac
     7501 tttcaggaag tcggcttttg gcgtaccgca gatgttttca acgacgtccg gcgtgtagcg
     7561 ggaaacgtgc gctttcagca ggttccacac acagcgcgga tgagtcagtg tttcatcgcg
     7621 ttttgcatag ccgttttcat cgaactgata gttccaggac gacttatcat actggcgttt
     7681 ttcagcgtcg tagccgctga acaggccgtc ttcgaaagca aaatcatcac gcaccagcag
     7741 gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt ttgttgtttt cgatcaggta
     7801 gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc
     7861 cgccacagaa gcggtacgcg taaaacgagg atcgacaacg atcaaggttg catcgttgtt
     7921 gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag cgttaccgcc
     7981 cattaccatt acgacgttag cgtttttgat atccacccag tggttggtca tcgcaccgcg
     8041 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc
     8101 taccgccagc atcccgaggg agcgggcaaa tttctgggtc agcatcccgg tttcgttgct
     8161 ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta cgccctgctc
     8221 gttcttttca ataaagttag cgtcacggtc agctttcatc agcttcgcaa tacgggagaa
     8281 tgcttcttcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat attccgggta
     8341 gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gccccttttg ggcacagcgc
     8401 accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt
     8461 tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac agtatgtgca
     8521 ggtgttacgg atctctttag cgcgtaataa tttgtagttt cgcgcctgag ccagtgcttg
     8581 cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg cgcagatttt
     8641 aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt attactgcgc
     8701 gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga acacgtgtat
     8761 gccacaaaat aatccgcctg cgcaaattaa cctacctcaa tagcggtaga aaaacgcacc
     8821 actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt taacggcaag
     8881 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
     8941 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct cggtccggtc
     9001 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
     9061 ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt attcgtcagc
     9121 tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca ggcgattgaa
     9181 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
     9241 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt cggcgtctgc
     9301 tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
     9361 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca
     9421 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
     9481 gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt tagcacgccc
     9541 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc tgcatggaat
     9601 gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt tacgcctgta
     9661 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
     9721 ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggttaa
     9781 aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa tattaaaaaa
     9841 taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc acatatcgat
     9901 aaataatatt atttcatcta tttttctcat tgtttttaat caccattcta ttttaaaaac
     9961 agtatgtcat ttataaatga cgccgtttaa aattcgttta gaaaatacgt ttaacgataa
    10021 ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg tggttgtaac tggtttgtta
    10081 gctgcgaacg tagcacacgc tgccgaagtc tataacaagg atggtaataa actcgacctt
    10141 tatggcaagg ttaccgctct acgttatttt actgatgata agcgtgacga tggtgataaa
    10201 acttatgccc gtctcggctt taaaggagaa acgcaaatca atgatcaaat gattggtttt
    10261 ggtcactggg aatatgattt taaaggctat aacgatgaag ccaacggctc gcgcggcaac
    10321 aagacccgtc tggcctatgc tggtttaaaa attagtgaat ttggctctct ggactatggt
    10381 cgtaactacg gtgtcggcta cgacattggt tcatggaccg atatgttgcc agaatttggt
    10441 ggcgata
//
LOCUS       DAZPJR010000150        10430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPJR010000150 DAZPJR010000000
VERSION     DAZPJR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.152"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHVB7_003427"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHVB7_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR1915322.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHVB7_003428"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHVB7_003428"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR1915323.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHVB7_003429"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHVB7_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR1915324.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHVB7_003430"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHVB7_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR1915325.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHVB7_003431"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHVB7_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHVB7_003432"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHVB7_003432"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1915326.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVTAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHVB7_003433"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHVB7_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR1915327.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHVB7_003434"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHVB7_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR1915328.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHVB7_003435"
     CDS             complement(9457..10149)
                     /locus_tag="ACHVB7_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR1915329.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2550 a         2516 c         2754 g         2610 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tgactgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt
//
LOCUS       DAZPJR010000151        10342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPJR010000151 DAZPJR010000000
VERSION     DAZPJR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.153"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(41..1045)
                     /gene="cra"
                     /locus_tag="ACHVB7_003436"
     CDS             complement(41..1045)
                     /gene="cra"
                     /locus_tag="ACHVB7_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR1915330.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1127..1213)
                     /locus_tag="ACHVB7_003437"
     CDS             complement(1127..1213)
                     /locus_tag="ACHVB7_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR1915331.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="ACHVB7_003438"
     CDS             complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="ACHVB7_003438"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1915332.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="ACHVB7_003439"
     CDS             complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="ACHVB7_003439"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR1915333.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3562..3606
                     /locus_tag="ACHVB7_003440"
     CDS             3562..3606
                     /locus_tag="ACHVB7_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915334.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="ACHVB7_003441"
     CDS             complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="ACHVB7_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR1915335.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5365..5451
                     /gene="leuL"
                     /locus_tag="ACHVB7_003442"
     CDS             5365..5451
                     /gene="leuL"
                     /locus_tag="ACHVB7_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR1915336.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5544..7115
                     /gene="leuA"
                     /locus_tag="ACHVB7_003443"
     CDS             5544..7115
                     /gene="leuA"
                     /locus_tag="ACHVB7_003443"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR1915337.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDITTAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7115..8206
                     /gene="leuB"
                     /locus_tag="ACHVB7_003444"
     CDS             7115..8206
                     /gene="leuB"
                     /locus_tag="ACHVB7_003444"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR1915338.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8209..9609
                     /gene="leuC"
                     /locus_tag="ACHVB7_003445"
     CDS             8209..9609
                     /gene="leuC"
                     /locus_tag="ACHVB7_003445"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR1915339.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9620..10225
                     /gene="leuD"
                     /locus_tag="ACHVB7_003446"
     CDS             9620..10225
                     /gene="leuD"
                     /locus_tag="ACHVB7_003446"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR1915340.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(10274..>10342)
                     /locus_tag="ACHVB7_003447"
     CDS             complement(10274..>10342)
                     /locus_tag="ACHVB7_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915341.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
BASE COUNT         2714 a         2718 c         2521 g         2389 t
ORIGIN      
        1 ccagagggaa attcacctgg cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac
       61 cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt
      121 ccaggcttgc caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca
      181 ctgccagcac cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg
      241 ccaggtcaga aggcagttta ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca
      301 acgcaaacga cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga
      361 ataactgggc agccacctcc cgctcatagc tgttggcata caggaaatgc acttcgcgcg
      421 gatcatcttt ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg
      481 gcagcgcgcc aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca
      541 tttcggcatc atcctgatcg gcaccaacca cgctggtgaa gtgttcacga tcgagggcgc
      601 ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag
      661 gaggcaacga cgtcgaaaca ataatggcat cgacctgacg ctgtaaaagg tgctcaatac
      721 agcgcatttc gttgtctggc tgatcttctg agcaggcaat tagcagttga taaccccgtt
      781 gccgcgcctg gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg
      841 ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg
      901 ggtggtaatt gtgctcacgc accacagcca tgactttttc aacggttttg tcgctcacac
      961 ggtattgctt cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca
     1021 gccgagcgat ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc
     1081 attgtactgg tatgggttaa attcattaac atctaagcgc ataaaattaa ctgcggcaac
     1141 tgcttttatt ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct
     1201 gcaaattgcg cattcagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac
     1261 cacaccagaa cgagcaactt ccacaatttt cgccacctcg cgaatcgatg ctaaaaatgc
     1321 atcaagctta tcgctggtgc ctgctaattg aacggtataa agcgagggtg tgacatcgat
     1381 aatttgccca cggaatattt ccgtattacg tttcacttcg tcacgcccgt aaccgctggc
     1441 ctgaattttc accagcatga tttcccgctc aacatgcgcg ccctgcccca actcactcac
     1501 gcgcaagaca tcgaccagct tgtgtaattg cttttcgatc tgctcaagta ctttttcatc
     1561 gcccacggtc tggatggtca tacgcgataa tgtcggatca tcggttgacg caacggtcag
     1621 gctttcaatg ttgtagccac gctgggaaaa aaggccaatc acgcgggata acgcgcctga
     1681 ttcattttcg agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt
     1741 aaccacattt catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcgctgcca
     1801 tcgacggtaa catcaacaaa caccaggcga ttattgtgca cctgttccag cgcctcgcta
     1861 agtttgcttt ccagctcatg cggatgagaa atctggatcc cgacgtgacc ataggcttcc
     1921 gccagacgga cgaaatcggg tagcgattgc atataagatt gtgaatgacg gccggaatag
     1981 atcatgtcct gccactgctt caccatcccc agatagcggt tattgagatt caccaccagt
     2041 acgggcaact cgtattgcaa cgcggtagac agttcctgga tgttcatctg aatactgccg
     2101 tcgccagtga cgcaaaccac ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca
     2161 ggtaaaccaa aacccatcgt gccgaggcca ccggaattga tccagcgacg cggtttgtcg
     2221 aatggataat aaagtgcagc aaacatctgg tgctgcccga catcggacgt cacgtaagcg
     2281 tctcccttcg tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg
     2341 tgagtgtcat atttcaggca ctgacgagcg cgccactgtt caatttgctg ccaccagtcg
     2401 cggatctcat ccagtggttg atgggcggat tcttgcgaca agagttcaag catttgttcg
     2461 aggacctggc gagcatcccc cacaatcggg atatccgcag tcacggtttt agaaatggaa
     2521 gtaggatcaa tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattgttc
     2581 gtcgttcggt catcaaatcg taccccgacg gcgaaaatca catccgcgtt atgcatcgtc
     2641 atattggctt cgtaggtacc gtgcattccc agcatgccca gtgcctgacg atgcgttgcc
     2701 ggaaacgctc ccagccccat caatgaggaa acaacgggca gattcaacgc ctccaccgtt
     2761 tctttcaact gctgatggca gcccgccgtg attgccccac cgcctacgta gacaaccggt
     2821 ttttttgccg ctaccagcgt ttgcagagcg cgcttaattt gccctttatg tccggtagta
     2881 gtgggattgt aagaacgcat actgaccgac tccggccaga catagggtaa tttgttcgcc
     2941 ggattaagaa tatctttcgg taaatcaacg actactggtc caggacgacc acttgccgcc
     3001 agccagaaag cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg
     3061 tgtttaacca ctggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct
     3121 atcaacgagg tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg
     3181 gtggcgatgc cagtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg
     3241 acttccccgg tcgcgcgcgc cagaccatcg gccatatgca ctgccgcctg ctcatgacga
     3301 actaaaacat gatcgatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg
     3361 cctccgggat aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc
     3421 tcggctccag acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa
     3481 cacattaacc gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgcagggtaa
     3541 taagaataaa caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggatta
     3601 aattgataat aaatcggctg aatcccacaa cttacagcaa tttctctatt tttttaatta
     3661 atcctaaaat agagagccca cacattcaga ataaaattct acattcaata aaaaatacag
     3721 aataaaccag acattcatgt ctgacctatt ctgcattcag ttagcgtttg caaattgaga
     3781 ctaattgctc ttccatccac tgatggcctt tatcgcgccc ggcagcttca tgccaggaga
     3841 gataacaggt tctgctattt tgttttaacg gcagcggtaa tacctgtaat tctaaggatt
     3901 cagcgaactc ttcagccagc caacgcggcg caatagcgac caaatgcgtt tgcgacacca
     3961 cgctaagtac gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg
     4021 tgtcatacca aggttgacta aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt
     4081 cgttataaac atcatgtttc agtaacgggc ccttaattgt tggatgattt ttgctggcta
     4141 ccagcaccat ttcatcttta aataatggca cgctggtaaa ttcaggacga tggaagtctt
     4201 cataactaat cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta
     4261 atgaagactt gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct
     4321 gcgaggtcag aatgctgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc
     4381 tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctgacgaa
     4441 ctgagccaaa aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa
     4501 aaagctcgtc attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg
     4561 acatacccag aacatgagcg gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa
     4621 cggttaataa gttgagatcg accatgcgta gctgtggctt gcttaactcc gccgtctctg
     4681 aatgatctgt ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc
     4741 ttattggaat gcagagaaat aatcctgaag aatatgattt atcatgcata taaaataaga
     4801 aaaagcaaaa tgagtaaaat tcggaaaaca taaagatacg ggttctgata aaaacagaat
     4861 ccattgccgt ggaatgagtc atttaccaca gcataagaat ccataatgtt tttgttgcga
     4921 aaacaatcta atcataacta ccgcgaatac tcaatcatct acaaaatgga ttaaatgtgc
     4981 ttatttaata attaatttat atatttaatg cattaattct taacattaat tgatcaataa
     5041 tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac aaatcatcag
     5101 gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat
     5161 ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc
     5221 ttactttaac caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg
     5281 gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
     5341 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
     5401 ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg
     5461 cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc
     5521 taaaagagac aaggacctaa accatgagcc agcaagtcat tattttcgat accacattgc
     5581 gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc
     5641 tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg
     5701 gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt
     5761 tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag
     5821 ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca
     5881 gcaccctgga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca
     5941 ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc
     6001 gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg
     6061 gctacaccat gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta
     6121 acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca
     6181 actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg
     6241 gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg
     6301 atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag
     6361 ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcagcgcat
     6421 tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa
     6481 tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg
     6541 ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt
     6601 tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt
     6661 acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc
     6721 tggattactt cagcgtgcag tctggctcta acgatatcac caccgccgcc gtcaaactgg
     6781 cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct
     6841 atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg
     6901 ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc
     6961 gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg
     7021 tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag
     7081 ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt
     7141 attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc
     7201 cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc
     7261 cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga
     7321 tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca
     7381 accagaacgc ggcgcgctgc tgcctctgcg taagcgcttc aaattattca gcaacctgcg
     7441 cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc
     7501 taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc
     7561 aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg
     7621 ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa
     7681 agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt
     7741 caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc
     7801 caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt
     7861 tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc
     7921 cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga
     7981 tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct
     8041 gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc
     8101 attagaagaa ggcattcgca ccggagattt agcccgtggc gctgccgccg ttagtaccga
     8161 tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg
     8221 ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg
     8281 ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt caatggtctg
     8341 cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac
     8401 gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag
     8461 gagctgatta aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat
     8521 caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt
     8581 gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc
     8641 ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa
     8701 accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg
     8761 ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc
     8821 ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc
     8881 gaaatgggcg caaaaaccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa
     8941 ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc
     9001 ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt
     9061 tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt
     9121 cccgatccgg cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa agcgctggcc
     9181 tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc
     9241 ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg
     9301 cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc
     9361 caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg
     9421 cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt
     9481 gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg
     9541 gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac
     9601 attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc
     9661 gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt
     9721 gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg
     9781 ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt
     9841 gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac
     9901 cgactacggc tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag
     9961 ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc
    10021 gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa
    10081 agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa
    10141 cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc
    10201 aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg gctttccgct
    10261 atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa
    10321 taaccgcaat tacccagtag ac
//
LOCUS       DAZPJR010000152         9894 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPJR010000152 DAZPJR010000000
VERSION     DAZPJR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.156"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            33..779
                     /gene="traX"
                     /locus_tag="ACHVB7_003448"
     CDS             33..779
                     /gene="traX"
                     /locus_tag="ACHVB7_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR1915342.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            834..1394
                     /locus_tag="ACHVB7_003449"
     CDS             834..1394
                     /locus_tag="ACHVB7_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1915343.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            1526..1738
                     /locus_tag="ACHVB7_003450"
     CDS             1526..1738
                     /locus_tag="ACHVB7_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1915344.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            1984..2445
                     /locus_tag="ACHVB7_003451"
     CDS             1984..2445
                     /locus_tag="ACHVB7_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1915345.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            2491..2700
                     /gene="hha"
                     /locus_tag="ACHVB7_003452"
     CDS             2491..2700
                     /gene="hha"
                     /locus_tag="ACHVB7_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR1915346.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            2738..3328
                     /locus_tag="ACHVB7_003453"
     CDS             2738..3328
                     /locus_tag="ACHVB7_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1915347.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            3483..3956
                     /locus_tag="ACHVB7_003454"
     CDS             3483..3956
                     /locus_tag="ACHVB7_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915348.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            4203..4352
                     /locus_tag="ACHVB7_003455"
     CDS             4203..4352
                     /locus_tag="ACHVB7_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1915349.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            4637..4885
                     /locus_tag="ACHVB7_003456"
     CDS             4637..4885
                     /locus_tag="ACHVB7_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR1915350.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            5130..5204
                     /gene="tap"
                     /locus_tag="ACHVB7_003457"
     CDS             5130..5204
                     /gene="tap"
                     /locus_tag="ACHVB7_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR1915351.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            5197..6054
                     /gene="repA"
                     /locus_tag="ACHVB7_003458"
     CDS             5197..6054
                     /gene="repA"
                     /locus_tag="ACHVB7_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR1915352.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            6755..6895
                     /locus_tag="ACHVB7_003459"
     CDS             6755..6895
                     /locus_tag="ACHVB7_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915353.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLP
                     CR"
     gene            6993..7646
                     /locus_tag="ACHVB7_003460"
     CDS             6993..7646
                     /locus_tag="ACHVB7_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFR1915354.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            7739..7996
                     /gene="pemI"
                     /locus_tag="ACHVB7_003461"
     CDS             7739..7996
                     /gene="pemI"
                     /locus_tag="ACHVB7_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFR1915355.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            7998..8330
                     /gene="pemK-mt"
                     /locus_tag="ACHVB7_003462"
     CDS             7998..8330
                     /gene="pemK-mt"
                     /locus_tag="ACHVB7_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFR1915356.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            8615..>9894
                     /locus_tag="ACHVB7_003463"
     CDS             8615..>9894
                     /locus_tag="ACHVB7_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR1915357.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFK"
BASE COUNT         2665 a         2169 c         2573 g         2487 t
ORIGIN      
        1 gggcggagac tgatacagga agataaacgc tgatgacaac ggataacacg aacacgacac
       61 gtaacgattc actggctgca cggaccgata cctggttgca gtcatttctg gtctggtcac
      121 ccggacagcg ggatatcatc aaaacggtgg cactggtgct gatggtactg gaccacatca
      181 atctgatatt ccagctgaag caggaatgga tgtttctggc cgggcgagga gcctttccgc
      241 tgtttgccct ggtgtggggg ctgaatctgt cccgtcatgc gcatatccgg caaccggcca
      301 ttaaccggct gtggggatgg gggattattg cccagttcgc atattatctg gcaggctttc
      361 cctggtatga ggggaatatc ctgtttgcct ttgcagtggc ggcacaggtg ctgacgtggt
      421 gtgaaacgcg cagtgggtgg cgtacagcag cggccattct tctgatggcc ctgtgggggc
      481 ctttgtccgg caccagttac ggcattgccg ggctgctgat gctggcagtc agccaccggc
      541 tgtaccgggc ggaagacaga gcggaacgtc tggcgctggt cgcctgtctg ctggctgtta
      601 ttccggcgct taaccttgcc accagtgatg cagcggcggt ggctggtctt gtgatgacgg
      661 tgctgaccgt tggtctggtg tcgtgtgcag gaaaatcatt accccgtttc tggcccgggg
      721 attttttccc ggtattctac gcctgtcatc tggctgtgct gggcgttctg gcgctgtgac
      781 gggtgtggta tctttgaccg taacaggatg attcgtcaga ggcagaacac agcatgacag
      841 agcagaagcg accggtactg acactgaagc gaaaaacgga aggggagacg cctgtccgca
      901 ggcggaaaac catcatcaat gtcaccacgc caccaaagtg gaaggtgaaa aagcagaagc
      961 tggccgagaa agcagcccgg gaagcggagc tggcggcaaa aaaagcgcag gccagacagg
     1021 cgctgtccat ttatctgaac ctgccctcgc tggatgaggc cgtgaacacc ctgaaaccct
     1081 ggtggccggg attatttgac ggtgaaacgc cccggcttct ggcctgcggt atccgggacg
     1141 tgttactgga agacgtggcg cagcggaata tcccgctctc gcataaaaaa ctgcgcaggg
     1201 cgctgaaggc catcacccgt tcagaaagct atctgtgtgc catgaaagcc ggtgcctgcc
     1261 ggtatgatac ggaagggtat gtgacggagc atatttctca ggaggaggaa gcgtatgcgg
     1321 cagcgcgtct ggataaaatc cgccgccaga acaggataaa ggcagagctt caggccgtgc
     1381 ttgatgagaa ataaaaaaaa gcctccatca gagaggaggc agggaaataa ggcttaaaag
     1441 gaaataactt ccgtacccga aaggaagcca attcagtaga ttatttaaca ccataatact
     1501 gacgggttaa agaggagtga taaagatgaa tggattcaga aacagttcac ggaacggtca
     1561 ggtctggcgc tggcagcgtg ccggaagccg ggccgttatt ctggaggtca gtggacgctg
     1621 gatggaagcg gcagaagcat ggcgacgggc tgccggtgtt gcgccccgga cagactggca
     1681 gcagtttgcc cgaaaaaggg ctgaacactg ccaccggcgc agccggggaa gggtgtaacg
     1741 aaaaaagcag ccttcattac tccagggatt cagaaggctg aataatgcat gagatttgct
     1801 ttttttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca gagttttttc
     1861 cgtattaagt cgtgatttta tattttgtga ttaatttcac aaaataaggt gttattcagt
     1921 gtgtgctgcg atattcagga ttacctgaaa tacaggtgcc atttatctga tatggagaat
     1981 aacatgagga aatatattcc actggtatta tttatctttt catggccggt attgtgtgca
     2041 gatattcatg gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca
     2101 cggaaggcag tgcggatcag gctggtaaat attgatgcac cggaaaagaa acaggattat
     2161 ggccggtggt caacggacat gatgaaatca ctggttgcag gaaaaacggt taccgtcaca
     2221 tattttcagc gagatcgtta tggtcgtatt ctcgggcagg tttatgcacc agacggaatg
     2281 aatgttaatc agtttatggt ccgggccggg gctgcctggg tatatgagca gtacaacaca
     2341 gatcccgtgc tgcctgtcct tcagaacgaa gccagacaac aaaaacgtgg gctctggtca
     2401 gatgctgatc cggttccccc ctggatatgg agacaccgta aataaagcga tggcaatctt
     2461 atcattcatc tgttctcagc gggaataaaa atggcgaaaa caaaacagga atggctgtat
     2521 cagttacggc gatgttcatc agtcaatacg ctggaaaaaa ttattcataa aaacagagac
     2581 agtttattaa acagtgaacg tgaatcattt aattcagcag cagaccaccg gttagcggaa
     2641 ttaatcaccg gaaagttata tgacaggata ccaaaagaga tatggaaata tgtaaggtaa
     2701 taattcagaa taattaatta ccaggagcag ataaacaatg aagctgatta tttttattct
     2761 cattgttctg attattgcag cgttacttat ccggattatc ctcaaaagtg taaatcagca
     2821 ttctcctttg ctgatgcagt tgcatgcggc aggtatacgg accggtgatg cagagcgaat
     2881 actgtccggc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
     2941 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
     3001 acgggtcgcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
     3061 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg aacggcgttc
     3121 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgccg
     3181 gcgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     3241 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     3301 tcagcagtcc ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     3361 aaacgggaaa aagcaagttt tccccgctcc cggcgtttct gtaataaaaa gcgggaaatg
     3421 gtggtcacag agtaaattcc ggaaccacct gtacccaaac tttatataat aatggtgaat
     3481 atatgaaata tgatgatgta tcagaattga tgattaagcg tgaacacaat attcttattg
     3541 ttgggaactc tgcctcccac attgataagt tttttaaggg gtattctata ccaggttcta
     3601 aatattatga tttcactcag attaacagtg attcagatgt agagagaaat gaggatgctg
     3661 tctcctttat cagggatgca atgaatagtg aagagcagac aatcattttt aattgtgctg
     3721 ggtggcctga tttaggtggt ggatcagcag tatcacagtt tgcaatgatg gtcagaaaat
     3781 ttggtaaaca acttattgtc gctgtaagtg aaaaggatgc catcaaatta aaagacaact
     3841 ttgatattat tgggatgttg tcatatggta aagaaaattt tattgccatg agtcatacga
     3901 aaaacgagtt aactggagga aaacgtcggt accggattaa aaatggatgt aaataaaaag
     3961 catacttatt ggctggagtg acgccttcgt gcttctgtag taaaaagcgg gcaatgcagg
     4021 taatagtcag caacagggaa tgtggtatta tcgcggcggg tgtctgagcc tttctggttc
     4081 aggcaagacg caggtaccag aaatgcaaag accccacttg ttaatccatt aactcgtgag
     4141 gtctgcatga agtacattgc actactgatt gtagcctctt ccttgcagag aggtcaaagt
     4201 ttatgacgaa gtatgccctt atcgggttgc tcgccgtgtg cgccacggtg ttgtgttttt
     4261 cactgatatt cagggaacgg ttatgtgagc tgaatattca caggggaaat acagtggtgc
     4321 aggtaactct ggcctacgaa gcacggaagt aaactgccgg gcggggacgg aagtccccgc
     4381 tttccggaag tgtgaggtat ttcaggggca gacacccgac atgccagaaa cagccggtcc
     4441 cgcccggggc cggctcccgg gttaaggcat ttcctgtttt ttcagccatt tcgcaacaaa
     4501 aatcacatta aacaacagta atccccgttg atttgtgcgc caacacagat cttcgtcaca
     4561 attctcaagt cgctgatttc aaaaaactgt agtatcctct gcgaaacgat ccctgtttga
     4621 gtattgagga ggcgagatgt cgcagacaga aaatgcagtg acttcctcat caggtacaaa
     4681 gcgagcatac agaaaaggta atcctctgac gcttgcagaa cgtcagcagg catccctggc
     4741 aagaaaacga gctacacata aagaactcag agtgttcatt cctgctgcgt taaaagcgca
     4801 attgcaggtg atgtgtgaag ctgagggcgt tacccaggct gagatgattg ccgaactgat
     4861 aaagcagaag agtgctttta gctaactaaa atgtagcgac atcacattct tgcctgtttc
     4921 tggtgtgcgt gatagattgc tgatcgttta aggaattttg tggctggcca cgccgtaagg
     4981 tggcagggaa ctggttctga tgtggattta caggagccag aaaagtgaaa accccgataa
     5041 tcttcgacaa gtttggcgac tgagaagatt accggggcca tctaaaaacc gtatagccaa
     5101 caattcagct atgcggggag tatagttata tgcccggaaa agttcaagac ttctttctgt
     5161 gctcactcct tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca acaaacgtat
     5221 taccgccagg taaagaaccc gaatccggtg tttacacccc gtaaaggtgc cggaacgctg
     5281 aagttctgcg aaaaactgat ggaaaaggcg gtgggcttca cttcccgttt tgatttcgcc
     5341 attcatgtgg cgcatgcccg ttcgcgtggt ctgcgtcgac gcatgccacc ggtgctgcgt
     5401 cgacgggcta ttgatgcgct cctgcagggg ctgtgtttcc actatgaccc gctggccaac
     5461 cgcgtccagt gctccatcac cacgctggcc attgagtgcg gactggcgac ggagtctgct
     5521 gccggaaaac tctccatcac ccgtgccacc cgggccctga cgttcctgtc agagctggga
     5581 ctgattacct accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc
     5641 acgttcacat ctgcactgtt tgctgccctc gatgtatcag aggaggcagc ggccgccgcg
     5701 cgccgcagcc gtgtggtatg ggaaaacaaa caacgcaaaa agcaggggct ggataccctg
     5761 ggcatggatg aactgatagc gaaagcctgg cgttttgttc gtgagcgttt tcgcagttat
     5821 cagacagagc ttaagtcccg gggaataaag cgtgcccgtg cgcgtcgtga tgcggacagg
     5881 gaacgtcagg atattgtcac cctggtgaaa cggcagctga cgcgcgaaat cgcggaaggg
     5941 cgcttcactg ccaatcgtga ggcggtaaaa cgcgaagttg agcgtcgtgt gaaggagcgc
     6001 atgattctgt cacgtaaccg taattacagc cggctggcca cagcttcccc ctgaaagtga
     6061 cctcctctga ataatccggc ccgcgccgga ggcattcgca cgcctgaagc ccgccggtgc
     6121 acaaaaaaac agcgtcgcat gcaaaaaaca atctcatcat ccaccttctg gagcatccga
     6181 ttccccctgt ttttaataca aaatacgcct cagcgacggg gaattttgct tatccacatt
     6241 taactgcaag ggacttcccc ataaggttac aaccgttcat gtcataaagc gccagccgcc
     6301 agtcttacag ggtgcaatgt atcttttaaa cacctgttta tatctccttt aaactactta
     6361 attacattca tttaaaaaga aaacctattc actgcctgtc ctgtggacag acagatatgc
     6421 acctcccacc gcaagcggcg ggccccgacc ggagccactt tagttacaac acacaaaaac
     6481 aacctccaga aaaaccccgg tccagcgcag aaccgaaacc acaaagcccc tccctcataa
     6541 ctgaaaagcg gccccgcccc ggcccaaagg gccggaacag agtcgctttt aattatgaat
     6601 gttgtaacta catcttcatc gctgtcagtc ttctcgctgg aagttctcag tacacgctcg
     6661 taagcggccc tcacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaccctcgt
     6721 cgggaccact ccgaccgcgc acagaagctc tctcatggct gaaagcgggt atggtctggc
     6781 agggctgggg atgggtaagg tgaaatctat caatcagtac cggcttacgc cgggcttcgg
     6841 cggttttact cctgtatcat atgaaacaac agagtgccgc cttccatgcc gctgatgcgg
     6901 catatcctgg taacgatatc tgaattgtta tacatgtgta tatacgtggt aatgacaaaa
     6961 ataggacaag ttaaaaattt acaggcgatg caatgattca aacacgtaat caatatctgc
     7021 agtttatgct ggttatgctg gctgcctggg gcattagttg gggagccaga tttgtcatgg
     7081 agcaggccgt tctgctttat ggatcaggaa aaaactattt gttcttcagt catggtactg
     7141 ttctgatgta cctgctgtgt gttttcctgg tataccgccg ttggatagct ccgctaccgg
     7201 tcgttggtca gctgcgcaac gttggcgtac cgtggctggt cggtgcgatg gccgtggtgt
     7261 atgtcggtgt atttctgctc ggtaaggcgc tggctctgcc tgctgagcca tttatgacga
     7321 aactttttgc cgataagtcc atacctgacg tgatcctgac gttgctgacc atctttatcc
     7381 ttgccccgtt gaatgaggaa acgctgttcc gggggattat gctgaacgtc ttccgttcac
     7441 ggtactgctg gacgatgtgg ctgggggcgc tgataacgtc gttgttgttc gtcgccgcgc
     7501 acagccagta tcagaacctg ctgacactgg cagaactgtt cctggtgggg ttgattacat
     7561 cagtggccag gatcagaagt ggtggcctgc tgctgccggt attgctgcat atggaagcaa
     7621 ccacgctggg tttactgttt ggttgaaagt tatattttta ttaaacattg tgcgttaaag
     7681 cctggtgtgt ttttttagtg gatgttatat ttaaatataa cttttatgga ggtgaagaat
     7741 gcataccacc cgactgaaga gggttggcgg ctcagttatg ctgaccgtcc caccggcact
     7801 gctgaatgcg ctgtctctgg gcacagataa tgaagttggc atggtcattg ataatggccg
     7861 gctgattgtt gagccgtaca gacgcccgca atattcactg gctgagctac tggcacagtg
     7921 tgatccgaat gctgaaatat cagctgaaga acgagaatgg ctggatgcac cggcgactgg
     7981 tcaggaggaa atctgacatg gaaagagggg aaatctggct tgtctcgctt gatcctaccg
     8041 caggtcatga gcagcaggga acgcggccgg tgctgattgt cacaccggcg gcctttaatc
     8101 gcgtgacccg cctgcctgtt gttgtgcccg taaccagcgg aggcaatttt gcccgcactg
     8161 ccggctttgc ggtgtcgttg gatggtgttg gcatacgtac cacaggtgtt gtacgttgcg
     8221 atcaaccccg gacaattgat atgaaagcac ggggcggaaa acgactcgaa cgggttccgg
     8281 agactatcat gaacgaagtt cttggccgcc tgtccactat tctgacttga acatggggtt
     8341 tgaggggcaa ctggatgaaa acgtacgatt tagggcacta aaaccgctgt tgtcccacca
     8401 ttctggtgat tcccaaacgt tatttggcta aaaagtagtt ttgatgtggt ttattttccg
     8461 aattccaagc gcagccctac tttcttgtgc gtatttgcgt gttttgcgca gttttgaagt
     8521 tcctacatca gattgacttc ggatacaaat tcaatgaaca ttgatgcaaa tactgaccta
     8581 tcagtttctt aactaaatac tcgagataca tctaatggct aatgaaaact tagatattga
     8641 ctccaagaca gcagaagaat tatacgagtg gtatctggat gataaattaa ttataaatcg
     8701 aagataccaa agaaaattag tatggtccct cgaagagaaa acagcactga tttcatcaat
     8761 gactcagcaa taccctatcc ctttgctatt gtttgtttcc attgacaaca aaagagaaat
     8821 ccttgatgga atgcaaagac tagaagcttt aatgagtttt attgagcagc gattttcatt
     8881 aaatggtaaa tactttaatt tagatgccat tgcattaaca aaacaactaa aagacagtgg
     8941 taaacttcaa caaaaagagc cagtgctatc ccgcgaaagt tctacattaa ttgctcgcta
     9001 tagatttgct atatctgaat actcttcaac agaggaccac attgacgaag tgtttcgacg
     9061 cataaattcc aatggtaaaa ttttatcaaa acaagaacta cgaagtgcgg ggtgcgttag
     9121 taatttttca gagttagtaa ggaaaatatc gacaattata agaggagata ctacacatag
     9181 cgatataatg ggactgaata aaatacataa catatcaatt tgcaatgatg gactcgatta
     9241 tggaataaat atagacaatc atttctatat tagaaatcat atcattagta ggcctagcat
     9301 tagagactct gacgatgaag agttagttgc aaatatcttg ggctatatat ttttagatga
     9361 taagccaaca tcaggatcaa catcgctcga tacattttac ggtgaaggtt cgacgagtca
     9421 tgcaatccat acacgaactc aattagagaa ttacattcag acaaatggtg cagataaaat
     9481 tgtcaataat tacctcttcg tttatgagat gattcaaaaa ttatttgatg caaataattt
     9541 gaactttaga tcacacattc tgggcaatgc atcttcatct caagaatgtc ctcgttacta
     9601 tcaagctgtt tttcttgctt tgtatgaatt aatcataaat gaaaacatgc aacttgacga
     9661 tgaacagaaa tttatcgctc agttaggaga cagtgtacaa cgttcaatgg tgcaaaccga
     9721 agggggccgt tgggcagcta gcgctcgtca gaaaagtgtc gaagatttat gtgctttaat
     9781 cagacgatat tttaaagaat cagaaaataa atttatcaat catgcttggc aaacactgat
     9841 aagaactcta cttaataact caagaacaga acaaccaaat tatgatttta aaca
//
LOCUS       DAZPJR010000153         9852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPJR010000153 DAZPJR010000000
VERSION     DAZPJR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.157"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHVB7_003464"
     CDS             complement(<1..163)
                     /locus_tag="ACHVB7_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR1915358.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHVB7_003465"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHVB7_003465"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1915359.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHVB7_003466"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHVB7_003466"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1915360.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHVB7_003467"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHVB7_003467"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1915361.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHVB7_003468"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHVB7_003468"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1915362.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHVB7_003469"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHVB7_003469"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1915363.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHVB7_003470"
     CDS             5058..6191
                     /locus_tag="ACHVB7_003470"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1915364.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHVB7_003471"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHVB7_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1915365.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHVB7_003472"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHVB7_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1915366.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHVB7_003473"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHVB7_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1915367.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHVB7_003474"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHVB7_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1915368.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHVB7_003475"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHVB7_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1915369.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHVB7_003476"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHVB7_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1915370.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..>9852
                     /gene="yjeJ"
                     /locus_tag="ACHVB7_003477"
     CDS             9762..>9852
                     /gene="yjeJ"
                     /locus_tag="ACHVB7_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1915371.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2518 a         2505 c         2502 g         2327 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg ca
//
LOCUS       DAZPJR010000154         9844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPJR010000154 DAZPJR010000000
VERSION     DAZPJR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.158"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(61..1056)
                     /gene="gapA"
                     /locus_tag="ACHVB7_003478"
     CDS             complement(61..1056)
                     /gene="gapA"
                     /locus_tag="ACHVB7_003478"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1915372.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1398..1811
                     /gene="msrB"
                     /locus_tag="ACHVB7_003479"
     CDS             1398..1811
                     /gene="msrB"
                     /locus_tag="ACHVB7_003479"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR1915373.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1853..2125
                     /locus_tag="ACHVB7_003480"
     CDS             1853..2125
                     /locus_tag="ACHVB7_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR1915374.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2286..2927)
                     /gene="pncA"
                     /locus_tag="ACHVB7_003481"
     CDS             complement(2286..2927)
                     /gene="pncA"
                     /locus_tag="ACHVB7_003481"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR1915375.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2938..3954)
                     /gene="ansA"
                     /locus_tag="ACHVB7_003482"
     CDS             complement(2938..3954)
                     /gene="ansA"
                     /locus_tag="ACHVB7_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR1915376.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4121..5977)
                     /gene="sppA"
                     /locus_tag="ACHVB7_003483"
     CDS             complement(4121..5977)
                     /gene="sppA"
                     /locus_tag="ACHVB7_003483"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR1915377.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6138..6689
                     /locus_tag="ACHVB7_003484"
     CDS             6138..6689
                     /locus_tag="ACHVB7_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR1915378.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6806..7849
                     /gene="selD"
                     /locus_tag="ACHVB7_003485"
     CDS             6806..7849
                     /gene="selD"
                     /locus_tag="ACHVB7_003485"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR1915379.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7854..9815
                     /gene="topB"
                     /locus_tag="ACHVB7_003486"
     CDS             7854..9815
                     /gene="topB"
                     /locus_tag="ACHVB7_003486"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR1915380.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2440 a         2459 c         2621 g         2324 t
ORIGIN      
        1 ccttaactga tcggcattac gaccgaagtc gctcttttta gatcacagtt tcatctcaac
       61 ttatttggag atgtgagcga tcaggtccag aactttgttg gagtaaccgg tttcgttgtc
      121 gtaccaggat accagtttca cgaagttgtc gttcagagcg ataccagctt tagcatcgaa
      181 cacggaagtg caaacttcgc cgttgaaatc ggtagatact acgtcatctt cggtgtagcc
      241 cagaacgcct ttcatttcgc cttcagcagc ggctttaacg gcagctttga tctgctcgta
      301 agttgcagct ttttccagac gaacggtcag gtcaactacg gatacgttcg gggtcggaac
      361 gcggaacgcc ataccagtca gtttgccatt cagttctggc agtactttac ctacagcttt
      421 agcagcaccg gtagaggacg ggatgatgtt ctgggaagcg ccgcggccgc cgcgccagtc
      481 tttgtgagac gggccatcaa cggttttctg agtagcggta gtagcgtgaa cggtggtcat
      541 cagaccttcg atgatgccga agttatcgtt gataacttta gccagcggag ccaggcagtt
      601 ggtggtgcag gaagcgttgg aaacgatgtc ctggccagca tatttgtcga agttagcgcc
      661 tttaacgaac atcggagtgt tgtctttaga cggaccagtc ataaccactt tcttcgcacc
      721 agcggtgatg tgtttacgag cagtttcgtc agtcaggaac agaccagttg cttcagcgac
      781 aacgtcaaca ccaacttcgt cccatttcag gttagccgga tcacgttcag cggtaacacg
      841 gattttttta ccgttaacga tcagatgacc gtctttcact tcaacggtac cgtcgaaacg
      901 gccgtgagtg gagtcatatt tcagcatgta tgccatgtaa tcagcgtcta acaggtcgtt
      961 gattgcaacg atctcgatgt cagaacgttt ctgagcagca cggaaaacaa tgcgaccgat
     1021 acggccaaaa ccgttgatac ctactttgat agtcatatat tccaccagct atttgttagt
     1081 gaataaaagg ttgcctgtaa aattacaaaa accttacgca gcgtcaagcg gaatcgtgtc
     1141 aatcattgcg acaaatcaat cctgtgccta agcattacgc gactgactcg cctcactctt
     1201 cctttgggct tgaaaccaca tggggtcggc gccccgaatt ttaaagggca attacgataa
     1261 aaatgtgatt ttcatcacga tttaggtgca gccatttcgt cgagatcaag tttaggacaa
     1321 aattttgcca gttggtgtta atgttttgtt agaatcagtc acgtaatgtg agcacgatta
     1381 aagtgagatg tgagcaaatg gctaataaac cttcggcaga agaactgaaa aaaaatttgt
     1441 ccgagatgca gttttacgtg acgcagaatc atgggacaga gccgccattt acgggtcgtt
     1501 tactgcataa caagcgtgac ggcgtatatc actgtttgat ctgcgatgcc ccgctgtttc
     1561 attcccaaac caagtatgat tccggctgtg gttggcccag tttctacgaa ccggtgagtg
     1621 aagaatccat tcgttatatc aaagacttgt cacatggaat gcagcgcata gaaattcgtt
     1681 gcggtaactg tgatgcccat ctggggcatg tcttccctga cgggccgcag ccaacgggcg
     1741 aacgttattg tgttaactct gcttctttac gcttcactga tggcgaaaac ggcgaagaaa
     1801 tcaacggttg aaaaaacgat tcagcaactt attccacagg agcggaaaaa ttatgaatct
     1861 tgatgacatt atcaacagca tgacgcctga ggtataccag cgtttgtcga ccgccgttga
     1921 actggggaaa tggcccgatg gcgttgcgtt gaccgaggaa caaaaagcga actgcctgca
     1981 actggtgatg ctgtggcaag ctcgccataa taccgaagca cagcatatga caattgacac
     2041 aaatggtcag atggtcatga agagcaaaca gcagttaaaa gaagattttg gtatctcagc
     2101 aaagcctatt gctatgttta agtgaatgag ttatctagta tttaagcctg ttattaaggg
     2161 agggcaaata tacttgttag gacgtcgtgt tttagctgca agtaggttaa atgcaacact
     2221 gacaagtatt atctggccta caatgtcatt gaggaaatgt aggccagaga tgaattcaac
     2281 taatattacc cctgtgtctc ttcccagtct gccagcgtat atagcgttgc cccagctgct
     2341 gacatctcca taaacgcgtg cgcactgtcc tggggctgga tattcacgcc acgacaacca
     2401 tcggtaatca catttacctt ataacctaac tgtaacgcgt ccagcacggt aaacttcacg
     2461 caatagtcag tagccaggcc cataacgatc aattcatcga tttcatgatc gcgtaaccag
     2521 tcatcgagcg aggttttctg ccgacggccg ttatcaaaaa aggcactgta actgtcaact
     2581 aaaggatttt cgcctttatg gaacaccgct gcgatcgctt tttggttcag taacggatgt
     2641 aattgtgcgc cttcactgtt ctgcacacag tgatctggcc agaaggtttg tggcaaaccg
     2701 tcgagttggc ctggcgtata aggctctaca ccgtgctgac tggcaaaact gccgtgattc
     2761 gccgggtgcc agtcctgact ggcgataacc gcttcaccgc gcgactggca ccagtcaatc
     2821 aggcggttag cgacatccac cgtactgtca ccttccggca cggcgagcgc gccaccagca
     2881 cagaaatcat tttgtaaatc gaccagtaac agggcgcgag ggggcattac agtctcctta
     2941 atcatccggc gtcagttcgc cgcgcaggtt ttggctcatg gccttgcgaa tggtttcagt
     3001 atccagttcc tggctcagca ggtaatgcag tttggttagc gtggcttcta cagtcatatc
     3061 tgcaccgcca ataacgccgg catgggcgag ggcgttaccg gtggcataac cacccatatt
     3121 cactttaccg gacatacatt gtgtcaggtt gaccaccaca ataccgcgat cgctggcttc
     3181 ttgtaattcc tgcaggaagg ctttgttttg tggcgcatta cccacgccat aggagcgcag
     3241 aatcaatgct ttcaccggtt ggcgcagaaa attgcgcacg acgtcagcag aaatccctgg
     3301 ataaatcgtc actacgccaa ttggttgtgg cgtgattgga tgaacgatta attccccttc
     3361 accgtgcggg gcgggtggcg tattcaaacg acgaatatgg ataccagctt ccagtaacgg
     3421 aggaaggttt ggagaggcaa acgcatcaaa accatcggca tgggctttgg tagtgcggtt
     3481 gccgcgatac aatcggttat tgaaaaagag cgttacttcg ttgatcggat aattcgccgc
     3541 aacgtacaac gcattcagca gattaatttg tccgtcagag cgtaactcag ccagcgggat
     3601 ttgtgaccct gtcacaatga ccggtttacc gagattctcg agcatgaacg acagcgcaga
     3661 ggcagtatac gccatcgtgt cggtgccgtg caggatgaca aaaccatcat agtcgtcata
     3721 gtgcgcttta atatcttcag caatatgctg ccagtcttct ggcgtcatat cagaagaatc
     3781 catcagcggc gtatattcat gaatggtgaa atctggcatc tccgggcgat ggaattccgg
     3841 catcagcgcc agttggcgtt gtagatgacc tgacaccgga atataaccct gctcggaacg
     3901 ctgcatcccg atggtcccgc ccgtgtaggc gacgtaaatt gatttctttt gcatgatatt
     3961 gatatgaatg atatcgaaag agcaaaagta tatacgtgag gcaggaggag cgcacctgat
     4021 actatgcgga taaggaaata tggggcgaga cagcgggaag aggattcgtc gacgtaaaaa
     4081 ataatgtggc cgacgatctg tcggccactc agtacaagac ttaacgcacg ttggcgcagg
     4141 tcaggcaaaa cgcataacgg ttttgtgggt cgttaaacgc ggccagcttg tcgctttcac
     4201 ttttaacggt agaggctacc gaggcaagcg gtgcaggtaa catggcctgg aacgcatctg
     4261 gcaacattgc ccggacagaa ccagacatgt tgtccatcac tttgtcgaag aaggtcggtt
     4321 catcaacgta gtattccaga tgccactgtt tcacttttgc cagctctgct gctttggcga
     4381 ccgcatcatc gaaatccccg agactatcga ccagcccgtt agcttttgca tcctgaccgg
     4441 tccagacgtg gccctgggcg attttatcaa tctgctccgg cgtcgaatga cgcgcatcag
     4501 caaccagcgt gataaagcgt ttatagccat tctcaatgct taattgcatc atctgctgcg
     4561 cttccggcgg cagtgccctg gtgatagaaa catccgccag cggtgaagtt gagacaccat
     4621 cagtatgaac accaatcgaa tccagactat tttctacggt ggtgatcacg ccgaagatac
     4681 cgatagaacc ggtcagggtg ctggggttag ccacaatgta attagctggc gtggaaatcc
     4741 agtaaccacc agatgccgcc atgccgccca tcgatacaac cacaggctta cccgctgccc
     4801 gggctgctgc cagttcagcg cgaatcactt cagacgcggt aacgctgccg cctgggctat
     4861 taacacgcag gacaatcgct ttcactttcg ggtcaaggcg agcgtcgcgg atttgtgccg
     4921 cagtggtatc accgccaaca ttcccctgag tttcctcgcc atccataatt gcgccattag
     4981 cgaagacgac accgatgctg tcaccggtat ctgccggcgt tttcaatgcg taatcgtaat
     5041 aactgatggc gcgataattt ttatcagtct tactccagcc gaactctttg gttagagctt
     5101 tttcgatttc ggcactcgat gccagtgcat cgaccagctt gttttccagt gcatatttcg
     5161 cggtatcgcc accggttttg gttaaaccct caagcaaccc ttgcgcgcca gggaatacct
     5221 gctgagcagg gatctgccgg ttagcggcaa cagtattcag atagttttgc cacagctcac
     5281 caatccagcg gctgtcagct tcgcgggctg ccggtgacat gtcatcacga ataaacggtt
     5341 caacggcaga tttatacgta cccacgcgga acacatgggt ggaaactttc agcttatcca
     5401 gcaacgattt gtagtacaga ccgttggtag caaaaccgtg caaatccacc acgccttgcg
     5461 gtgacagcca aattttattg gcgaaactgg cgagataata ttgcccctgg ctgtagttct
     5521 cgccaacggc ataaaccggt ttcccgctgt cacgaaactc tttcagggct ttgccgatgt
     5581 actgcataga cggctggtcg ccgcctgcga agtttttcag atccatcacg ataccggtga
     5641 tattgcggtc gtccttcgcc tggcgaatgg tgttgacgat atcaaacagt gagttttcct
     5701 gcagacgatc ggaactggca ccaagcagct ggcggcttaa tttactaaac cgctgagaac
     5761 tgtcgggttt atcgacgatc acaccagaaa tgtccagcag cagtgcgcca cgactggccg
     5821 tttctttcga atcaccacca ctgacctgca tccagatccc cacaccaacc agtacgagga
     5881 aaataaagaa caggttaagt accatttcac ggacgaaatt cagcagacgc cacgtccatt
     5941 taaaaaatcc ggcaataaat cgccaaaggg ttcgcatgta ttctcccaac ttaaggtcac
     6001 acctgtcaca ggcgcaatag atgtggttat cgtaatgaca caaccggcac ttgtcagcag
     6061 gaattgcgga ctgtgctgta acaaaatcca ctctcgtgtt aattttgtga ataaatatca
     6121 cgacaggagt taatcaaatg gatgcactcg aactattgat caatcgccgt agcgcctccc
     6181 gcctggctga acccgcgcca acgggtgaac aactgcaaaa catcctgcgt gcgggtatgc
     6241 gtgcgccgga ccataagtcc atgcaaccgt ggcatttttt tgtgattgaa ggggaagggc
     6301 gcgagcgttt cagcgccgta ctggaacagg gggcgattgc tgccggtagt gatgacaaag
     6361 ctatcgacaa agcccgtaat gcgccgttcc gcgcaccgct catcatcacg gtggtggcga
     6421 aatgcgaaga aaatcataaa gtcccgcgat gggaacagga aatgtctgcc ggatgcgcgg
     6481 tcatggcgat gcaaatggca gccgttgccc agggatttgg cggcatctgg cgcagtggcg
     6541 cattaactga aagtccggta gtgcgtgaag cattcggttg ccgtgagcag gataaaattg
     6601 tcggttttct ctacctcggc acgccgcagt tgaaagcatc tacgtcgatt aacgtcccgg
     6661 acccgacgcc gtttgtaact tatttctgat aattgtcgcg aaactgtctg gattgtgagc
     6721 aaaggcgcag gaattcagac actctcactt atcacttcac ggaatgaggg ttaccatacc
     6781 cggattgcat tgacaggaga tgtccatgag cgagaactcg attcgtttga cccaatacag
     6841 ccacggagct ggttgcggct gtaaaatttc cccaaaagtg ttggaaacca tcctgcacag
     6901 tgagcaggcg aagtttgttg atccgaattt gcttgtgggt aatgagaccc gcgacgatgc
     6961 ggcggtgtac gatctgggca atggcaccag cgttatcagt accaccgact tctttatgcc
     7021 gatcgttgat aatcctttcg attttggccg cattgcggcg actaacgcca tcagcgatat
     7081 cttcgcgatg gggggcaaac cgattatggc gattgcgatc ctcggctggc cgattaacaa
     7141 actttccccg gaaattgccc gcgaagtaac cgaaggtgga cgctatgcat gccgtcaggc
     7201 gggtattgcg ctggctggcg gtcactccat cgatgcgacg gagccgattt ttggtctggc
     7261 ggtaacgggg atcgtaccga ccgagcgggt gaagaaaaac agtaccgcac aagccggatg
     7321 caaactgttc ctgacgaaac cgctggggat cggcgttctt accacggctg agaaaaaatc
     7381 actgctgaaa ccagaacatc agggactagc gacggaagtg atgtgccgga tgaacatcgc
     7441 aggcgcgtcc tttgccaaca tcgaaggcgt aaaagcgatg accgacgtta cgggttttgg
     7501 tctgctgggc catttgagcg aaatgtgtca gggcgcaggt gtgcaggcgc gcgttgacta
     7561 tgacgcgatc ccaaaattgc cgggggttga agagtacatt aagttgggcg cagtgcctgg
     7621 cggcaccgaa cgtaactttg ccagctacgg tcatctgatg ggtgaaatgc cgcgtgaagt
     7681 gcgcgatttg ctgtgcgatc cgcaaacttc tggcggtttg ctgctggcgg tcatgccgga
     7741 agcagaaaat gaggtcaaag ctacagccgc cgagtttggc attgaactga cggcaattgg
     7801 cgaactggtg ccagcgcgcg gcggtcgtgc catggttgag attcgttaat tcaatgcggt
     7861 tgtttattgc cgaaaaaccg agtctggcgc gcgccattgc tgatgtcctg cccaaaccgc
     7921 accggaaagg cgatggcttt atcgagtgcg gtaatggtca ggtggtgacc tggtgtatcg
     7981 gtcacctgct tgagcaggcg cagccagacg cctacgacag ccgctatgcg cgctggaatc
     8041 ttgcggattt gccgattatc ccggaaaagt ggcaattaca gccccgaccc tccgtgacca
     8101 aacaacttaa cgtcatcaaa cgcttcctgc atgaagccag cgaaatcgtt cacgccgggg
     8161 acccggatcg tgaagggcaa ctgctggtgg atgaagtgct ggactatctg caactggcac
     8221 cggaaaagcg ccagcaggtg cagcgttgct tgataaacga cctgaacccg caggcggttg
     8281 agcgggcgct cgaccgtctt cggtccaaca gtgagtttgt gccattgtgc gtttctgcgc
     8341 tggcgcgagc gcgtgccgac tggctgtacg gcatcaatat gacccgtgcg tataccattc
     8401 tcggtcgcaa tgccggttat cagggcgtac tttccgtggg acgcgtgcag acacccgtgc
     8461 tcgggctggt ggtgcgccgc gatgaagaga ttgaaaactt cgtggcgaaa gacttctttg
     8521 aagtcaaagc acatatcgtg acacctgccg atgagcggtt taccgctatc tggcaaccga
     8581 gcgaagcgtg tgaaccgtac caggatgaag aagggcgctt gttacatcgt ccactggcgg
     8641 agcatgtggt taaccgcatt agtggtcaac cagcaattgt caccagctat aacgataaac
     8701 gggaatcaga atccgcgccg ctgccttttt cgctttcggc gttgcagatt gaagcggcaa
     8761 aacgctttgg tctgagcgcg cagaacgtgc ttgatatctg ccagaagctg tacgaaacgc
     8821 acaagctaat cacttatccg cgttccgatt gtcgctattt gccagaagaa cattttgccg
     8881 gacgccacgc ggtgatgaat gccatcagcg ttcatgcacc agatctgttg ctgcagccag
     8941 tggtagatcc agatatacgc aaccgctgtt gggatgacaa aaaggtcgat gcacaccacg
     9001 ccatcatccc gaccgcacgg agttcagcca tcaacctgac ggagaacgaa gcgaaggtct
     9061 ataacctgat tgcccgtcag tacttgatgc agttctgccc ggatgtggtg ttccgcaagt
     9121 gtgttatcga actggacatt gccaaaggca aatttgtcgc taaagcgcgt tttcttgctg
     9181 aagcaggctg gcgcacgctg ttaggcagca aagagcgcga tgaagaaaac gacggtacgc
     9241 cattgcctgt ggtggcgaaa ggcgatgagt tgctgtgtga aaaaggtgaa gtggtagagc
     9301 ggcaaaccca gccgccgcgc cattttaccg atgcaacact gctttcggcg atgaccggga
     9361 tcgcgcgctt tgtgcaggac aaagatctga aaaagatcct tcgtgcgacc gatggtctgg
     9421 ggacagaagc aacccgtgcc gggattattg aactgctgtt caagcgtggt tttctgacca
     9481 aaaaagggcg ctatatccac tctaccgacg ccggaaaagc gttattccat tcgctgccag
     9541 aaatggcgac gcgaccggac atgaccgcgc actgggaatc ggtgctgacg caaatcagcg
     9601 aaaagcagtg tcgctatcag gactttatgc agccgctggt ggggacgcta tatcagctta
     9661 ttgatcaagc caaacgtacg ccggtgcggc agtttcgcgg cattgtggct ccgggcagtg
     9721 gtggcagtgc tgataagaaa aaggctgcac cgcgtaaacg tagtgcgaaa aaaagtccgc
     9781 cagcagatga agtcggaagc ggggcgatag cgtaagcgag tgaatctttc gtgctattcg
     9841 agtc
//
LOCUS       DAZPJR010000155         9649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPJR010000155 DAZPJR010000000
VERSION     DAZPJR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.159"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..671)
                     /gene="ydcF"
                     /locus_tag="ACHVB7_003487"
     CDS             complement(<1..671)
                     /gene="ydcF"
                     /locus_tag="ACHVB7_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR1915381.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDS"
     gene            complement(943..4845)
                     /gene="hrpA"
                     /locus_tag="ACHVB7_003488"
     CDS             complement(943..4845)
                     /gene="hrpA"
                     /locus_tag="ACHVB7_003488"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR1915382.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5046..5651
                     /gene="azoR"
                     /locus_tag="ACHVB7_003489"
     CDS             5046..5651
                     /gene="azoR"
                     /locus_tag="ACHVB7_003489"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR1915383.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5702..7018)
                     /locus_tag="ACHVB7_003490"
     CDS             complement(5702..7018)
                     /locus_tag="ACHVB7_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR1915384.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7008..8765)
                     /locus_tag="ACHVB7_003491"
     CDS             complement(7008..8765)
                     /locus_tag="ACHVB7_003491"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR1915385.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8781..9649)
                     /locus_tag="ACHVB7_003492"
                     /pseudo
     CDS             complement(8781..9649)
                     /locus_tag="ACHVB7_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2449 a         2629 c         2274 g         2297 t
ORIGIN      
        1 tcgctatcat cgcgtaaacg cggcagctcg ccagtgagta gtgagagata acgctcaacc
       61 ggccataatc cttgtaactg attaataaag attacactgt ctgcgttatt tcctaactga
      121 ggaacgaatc cgggataact taaccagcgt ggtgcatcgg gattgtcccc agtcatacgg
      181 cggaacgtcg ccatcgtgcg ccgctgcatg gtggggtcct gaacaacgat agccgtatga
      241 actcgttcta cggcctgatt caatagcgcg atgctaaagc gtgcgttttc accgcagttt
      301 gttgactggt cttcaatcca gattttttca tgcggaatgt gccagaactg atgagcgata
      361 tccgcctgga tggtcgcttc tgctctgcca gtggtgcgga tagtgttgta gtgcggatgc
      421 tgtgcgatgg cgctatacaa aaaagttgtc gagtgaccga taccaccact aatcagtaaa
      481 ggaatttgct gatcgcgagc aatcttacat gccgcgtcga tagtcggcat aaccgcattg
      541 cctgcaagga tcacgcaatc ggcctgatac ggcacctcac cggagaaatc atcctgcgcc
      601 agccactgtc cgataacatt tatggcatct atagttgccg gcgaaagcgt cggaaacggg
      661 gtaatgttca tggttcctcc ttatcgttcc cttaagaata ctgcgtgaga taatagtgag
      721 aagcggagca ctctgaaagg aggaagtcgt atttccttaa ccgccactcc gcaagcctga
      781 catatctttt aaggaaatgc tgtagataaa cattattcat cttgcagcag agaattgtat
      841 ttttctgaat tcctgaattt ttacccttac cgttttagaa ttaataaaaa acccggcgct
      901 aaatgcttac cgggtttttt ctttatcagg caaatagcag ggttaaccgc taatctgctc
      961 catcgcctgc aaaatacgct tatctgaaat cggataaggc gtaccaagtt gttgagcgaa
     1021 gtaactaaca cgcaactctt ctatcatcca acggatctct ttcacgtctt catcctcacg
     1081 acgtgcaggc ggcagtttgt tgatccattg ctgccacgcc tgctggacgt tttcgacttt
     1141 cagcatctga gcacggtcgc gatgtggatc aaccgccagt ttttccagcc gtttttcaat
     1201 cgcctgcaaa tatcgcagcg tgtcgcccag ccgtttgaag ccgttaccag tgacaaaacc
     1261 gcgatatacc agcccgccca tctgcgcttt aatgtcagaa agccccagcg ccatcgtcat
     1321 atccacccgc cccttcagac gtttgttgat attgaacact gccgtaagga tttgctcgac
     1381 ctgcttcgca atatccacca ccgtgtcgtt cagttcggca cgcacttttt catgcagcgc
     1441 agcaaagcct tcttccgtcc agaccgggcc accattggcg tcgatcaatt tatccacacc
     1501 gcaggagata cagtcgtcga tcagctccag cactttgcca tacgggttaa agtacagccc
     1561 cagcttggct ttgtttggta acttctcatg taaatacttg atcggcgagg gaatattcag
     1621 cagcagtaga cggcgaagac cgttccacat tacctgtttt tgctccagcg gattatcaaa
     1681 cagtttgatc gccacactgt cgcgttcatc cactagcgct ggccacgcct tcactttgta
     1741 gttgccacgc ttctgttcgt agctttccgg cagctgacca aaactccaga tatgcagtcc
     1801 gctctgctcg ataccgtcat ccgccaccgc agacagcgtt tcctgcactt tgcctttcag
     1861 cgcatctttc agatcttgta gcgagcgccc ttcttttagc ttcttgtttt tgtcatccac
     1921 cacgcggaag gtgattttca ggtgatcggg cacctgatcc cagtgccagt cttcgcggtc
     1981 aacggtaacg ccggtcatcc gccgtaactc gcgctcaagg ctgtcgagca acggtaactc
     2041 cagcggtgtg acgcggccta aaaacgcttc ggcgtagttt ggtgcaggca caaaattacg
     2101 gcgtaccggt ttcggcaacg atttaatcag agcaatcacc aattcgcggc gcagaccggg
     2161 gatctgccat tcaaacccgc tttcctcaac ctggttaagt aacggcagcg gaatatgtac
     2221 ggtcacaccg tccgcgtccg cgcctggctc aaactgatag ctcaaacgca gcttgagatt
     2281 gccctgatgc cagaagttcg ggtaatccag ctttctgatt ttttccgccc cttctttgat
     2341 caacatgctc ttttcaaagt tgagcaaatc aggcgtttcg cggctgactt ttttccacca
     2401 gctatcgaag tgacgagcgg agattacatc gtggctgatg cgctggtcgt agaactcaaa
     2461 cagcgtttcg tcatcaacca gaatatcgcg gcgacgtgat ttgtgttcca gctcttccac
     2521 ttcggcccgc agtttcaggt tttcacggaa gaatgcgtga cgcgtctgcc agtcaccttc
     2581 caccagagcg tggcgaataa agagttcacg acataacgcc gaatcgatct ggctgtagtt
     2641 gaccttgcgc gcagcaacaa tcggcaaacc ataaacagtg actttttccg ttgccatcac
     2701 cgcgccctgc gcccgttccc agtgcggttc gctgtaggtg cgtttaatca aatgctgagc
     2761 aacgggttct acccattccg ggtcgatacg cgcagcaatg cgcccccaca ggcggctggt
     2821 ttctaccagt tccgccacca ttacccattt cggcggcttt ttgaataaac cagaaccggg
     2881 gaagatggag aaacgcgcgt tacgtgcacc ggtatattcc tgtttatcgg catctttcat
     2941 gccgatatgg gaaagcaaac cggtcagtaa ggcgatgtga atttcacgat actccgccgg
     3001 ttcgctgttg accggaatgc caagttcttt caccacctgg cgcaactggg tgtagatatc
     3061 ctgccattcg cgcacgcgca gatagttgag gtaatcggta cgacacaggc gacggaaggc
     3121 gttggaagaa agcgcctttt gctgctcgcc aagataattc cacagattta caaacgcgag
     3181 gaagtcggat tctttgtcgt ggaagcgacg atgtttttcg tccgatgcct gctgtttgtc
     3241 catcggacgt tcgcgcggat cctgaatgga gagcgcggac gtgataatca tcgcctcacg
     3301 cacgcagcca tgtttttgcg cttccagcac catacgcgcc agacgtgggt cgacaggcaa
     3361 ctgcgagagc tggcgaccga gcggcgtcag tttataggcg ctggcctgtt catccgtggt
     3421 gatcgcgccc agttcttcga gcagacgcac gccatcctgg atattgcgtt tatccggtgc
     3481 ttcgacaaac gggaacgcag cgatatcgcc cagccccagc gcggtcatct gcaaaataac
     3541 cgaggccagg ttggtacgca gaatctccgg atcggtaaac tccgggcgcg agaggaaatc
     3601 gtcttcggaa tagagacgaa tacagatccc ttcggacaca cgaccacagc ggcctttacg
     3661 ctgattggca gacgcctgcg aaatgggttc aatcggcaaa cgctgcactt tggtgcgata
     3721 gctgtagcgg ctgatacgcg ctgtaccggg gtcgatgacg tatttaatcc ccggtacggt
     3781 cagcgaggtt tccgcgacgt tggtcgccag cacaatgcgc cgtccgctgt gcgactggaa
     3841 cacgcggttc tgttcgctgt tcgaaagccg cgcataaagc ggcaagattt cggtatggcg
     3901 taagttcagc ttgttcagcg catcggcggt atcgcgaatt tcccgctcac cactcataaa
     3961 gatcagaatg tcgccagggc tttcctgact cagttcgtct acggcgtcaa aaatcgcctg
     4021 caactgatcg cgctcggtgt catcggcttc ttcaacaatc ggtcgatagc gcacttccac
     4081 cggataggtc cgaccggaga cttcgataat cggcgcatta ttaaagtggc gcgaaaagcg
     4141 ttccgggtcg atagtcgcgg aagtgataat gatttttagg tcaggacgcc gcggcagcaa
     4201 ctctttcaaa tagccgagca gaaaatcgat attcaggctg cgttcgtgcg cttcgtcaat
     4261 aatgatggtg tcgtactgca tcagcaggcg gtcttgctgg atctccgcca gcaggatacc
     4321 gtcggtcatc agcttgacca tcgtgttatc acttacgtga tcgctgaaac gcactttgta
     4381 accgatgcaa ccgcccggct ccgttttcag ctcttccgca atacggttcg ccaccgttct
     4441 tgccgccagg cgacgcggct gggtatggcc gatcagccct ttaatcccgc gccccagctc
     4501 catacagatt ttcggtaact gagtcgtttt accagaaccc gtttccccgg cgacgatcac
     4561 cacctggtga tcacgaatcg cttcgagaat gtcctgtttt ttctgactaa ccggtaaatt
     4621 gtcaggataa gtaatttccg gtcgtgccgc ttcacgcagc aggactttcc ctgccgcctg
     4681 gtcaatctct ttcgccatct cctggaaaat ggcctgttgt gcatcaggat ttttaacctt
     4741 cttcacgccg tgcagacggc gagaaaaacg cagtctgtca cgcagcatca gcgaatccag
     4801 ccgctgctgc aaggccgtaa aggtcaattt ttgttgttct gtcataacgt tagtgggcag
     4861 tagtctgcct gattaatttt ctttgaaatg atagatatag agtaccacat cacggctttc
     4921 tatgcgttgt tcaataaatt cgaacatagg cttcgattta ttgcgctatc tctgtggcag
     4981 gattgtgaat aaagtgtcaa caagcaacgg ggcatcgccc attcaaacat ctataaggaa
     5041 acaccatgag caaggtatta gttcttaaat ccagcatcct ggcagggtac tctcagtcta
     5101 atcagttgtc cgattatttt gttgaacaat ggcgcgaaaa gcactccgct gatgaaatca
     5161 ccgttcgcga cctggctgca aatccgattc cggtactgga tggcgaactg gttggtgcac
     5221 tgcgtccgag cgatgcgccg ctgactccgc gtcagcagga agctctggcg ctttccgatg
     5281 agctgattgc cgagctgaaa gcccacgacg ttatcgttat tgcggcaccg atgtataact
     5341 tcaacatctc aactcagttg aaaaactatt ttgacctggt tgcacgcgca ggcgttactt
     5401 tccgctatac cgagaacggt ccggaaggtc tggtaacggg taaaaaagcc atcgttatta
     5461 ccagccgcgg tgggatccac aaagatggac caacggacct ggtgacgccg tatctgtcca
     5521 cgttcctcgg ctttatcggc attactgatg tgaaatttgt cttcgccgaa gggatcgcat
     5581 acggtccgga aatggcagcg aaagcgcagt ctgacgcgaa agcagccatc gacagcattg
     5641 ttgctgcata accggacacg atcttgatgt ggactgtagt ccagtccaca tcccaatcca
     5701 cttacctgtt ttcccataat ttcagcatcg ctttgtgatc ttccttaagc acaatatgcg
     5761 agcgtctggc tcgaataaag ctaatcgctt catcaacggt tttacagtat ccgtaacata
     5821 acagccatgc cgccactacc agcgcactgc gcgataatcc cagtgcgcaa tggaccagaa
     5881 cgctgccttg ctcttcgcgt aatgtttcca gcatcgccac ggcctgtcgg agctccccct
     5941 cttccggaac caccagatct agcatcggta cacagaaata gagtcgatct tttgtcgctc
     6001 gtccacgagg gaattcaaag gtgacgtcca gaaccgcatt ctgtgccgga atatgtcgtg
     6061 gaaacgcccc taaataaaca ccagcagtca ttttgctcac cggctccagg cgacgacaaa
     6121 accaacgcat agatagccac atcccgatgc gccagggcaa tgtcagccag taaacggcgg
     6181 gcgggagttt cccctgacta tctttccctg ttgttatcgc gccaagccca ccgtacccac
     6241 ggccaataat gagtagcgat aatactggcc aacataacca gactgaccac cataactgaa
     6301 ccatcatcat tagctccatc aacacaatgc acgcgcacgc gcctacgaca tatggcagtg
     6361 ctattttgat tcgacgttga tcaggtttct gataattcca acgacggtcg acgggcacca
     6421 tccagtcaat caacatacct accgccagcc ctgtgatgac atcaataaaa tgatgctgcc
     6481 atgtcgtcag cgtcgaaatg gcgatgagta aaaaccatcc gccgcagact ttacgccacc
     6541 tcacagccag atgctgacga aagtgacgcc aaagtagcca gcagagaata atatgcagcg
     6601 aaggagactg gttataaggc agatcaaaca gttcaagttg cgaaaatagc catcccgtca
     6661 ccccactcac ttcaggacgg ataaaactaa acttcagcgg atagagcaaa aaaccgcagc
     6721 aggccattac cgttgccaga ataagccggt ggacaagtcg gcgctgttcg aatgtcgtgc
     6781 tacaaacgaa cagcgaaaat ccatataaaa gatccagact ccagtaagga acaatagtcc
     6841 agggaagaaa agggatcgcc gtttcccaac cgaatacctg actgggaata tcatggctat
     6901 tactgtcctg aaccgcggtg aactgattaa gagatccata ggtgaagaag aaaaacgggg
     6961 ccagcaacaa taaccagcca gctccttgta gcaatacgtt ccggcgttca gttatcacga
     7021 cgcaccgcca tcgaaaccgt aaaaatgccc cactcatcaa tccgttgtgt gcatttatca
     7081 aatccagcat catgcacgag caaatccatc tccccttgcg tacgcacgcg cattacccac
     7141 agtttaccat ctttatgact ggttaacacc ccggcaatca gctccagttg tgggtgccac
     7201 ggctgcccgg tgtagatgag aatgccaccc ggttcgatgg cattggcaag acctgcgagt
     7261 gagtttttta cctgctcatt ttcgggaaac agctcataca gtccagagac aatcgccagc
     7321 gtaggccgcg gagttaacgc gctgagttcc tccgggttaa acgcatcgcc ctgttcaaaa
     7381 cgcacccgcc cagacattcc ccgttgggca atcatctctt gcccctgtgc aacatttaac
     7441 tcactgtaat cacgtaataa aatatcgctt acggcaggct cgtttgccag tgcatccagt
     7501 acatagcgcc catgccctgc ggcaatgtca accacgcgga cggctaaacc tttgacgtgg
     7561 agatcggcaa cggcttgttt aatcagtatt tgtaaatgag ttttgcgctg gcgaataccg
     7621 cgccagccaa cactgttcag gtaaattttg tcgattaagc gcccgaatgc gttactgcct
     7681 tgcggttgat tttgatagac ataatccagc gaactgcctg aatcaaagcc agtgctcatt
     7741 ccgagatgca ggcccgcaga gtgcgtcccg aacaatttca tcgctttacg cataaagcga
     7801 tacgccaaat caaccggcga taatggcacg ggtccacctg aaaggaggcg ccagcgatcc
     7861 gctgatggtc cagtgcggtc ttcatgctga taatcaaatt tttgtgactt gttagcatat
     7921 aaacgacgaa taaagctttg cattttttca aatgcctgcg ccctgttctc ttcacccaac
     7981 gtgtcgtgat aaaagcctgg cagcagatgc agctctttca gagggctacg taatctctga
     8041 taaaaatcaa tctgcggttg gcgatgcacc acatagtcat cgcctgatat cagaagttgc
     8101 gtggggagcg taatcgccgc cgcatctcta ataatacgtt cagacgtttt atagagatcg
     8161 agcaagatat tgacggcaat cgcccgtgtg atcagcggat cattattgaa actcgccacc
     8221 cgttgccgat cgtgggtcaa atagcgtctt ttcacatagg aattaataaa aaacagacca
     8281 cgcagacgat gccatagcgt cagcgcagga cgtgccagcg gcacatacaa tttaacctta
     8341 aaggccggag aagccagcac cagcccgcga attgcaggcg cataatcatg aacccatgtg
     8401 gcaaccagca ctgcgccgac gctttgcgcg atcacaacca cctcttccag tccaacctgg
     8461 ctgtcgctgg cagcaaaacg gacaaattca tcgacatcct gcactgaacg cgcaagagat
     8521 gggctataac cacgcggccc cgaagttttt ccatgccccc gggcatccca tgcataaaaa
     8581 gcagtatctg gcatcgccag ttcatcaacg atatgttgta gacgaccaga atgttcatgg
     8641 ccacgatgaa ataagacgat gacctttttc gcccctggct gtaaagcggg ccagtgccga
     8701 taaaacaacg ctgtattatc actggtggta aaaaaatgtt ccccagagat gcgtgaattt
     8761 tccattttct ctattttcct tcagtaacag cagtaacgaa taaaataaaa aaataccgga
     8821 gcggtgaaaa ttaatgagtc aatcctgtca aggattccgc catgtccagg taatagtttc
     8881 ccgctatctt taacaccaac atctcgtttg atggctgaca tcactacatc gccgcaaaaa
     8941 ccactaatac caattaacaa acccgctaat attgcctgta atgtatttag cggtgtcagt
     9001 aacggtccaa tgatcatcga cgctatcatg gtggtgatta cgccgccgac cagaccttcc
     9061 agcgtttttc ccgggctgac tttggggacc acttttcttc tgccgcagga tttcccccat
     9121 aaatactgtg caatatcgtt tgacgcggtt aatgccaaaa gaaataacac cagtaaggcc
     9181 ccgccctgga tattcatgga tggcaacatt aataactagg cggcatgact caaggcgaaa
     9241 acggtcatta tccagcccca aaaaatggcg ctcacggtat gcagaaaacc ggaggggtca
     9301 cccacaaaaa ctcgccaggc ggctaatatc agaaacccag ccaaaggtat aaataacaga
     9361 aacagctcaa agcaattaaa accgataagc aaatagttaa gaggaataac ccaatataac
     9421 caccgcggaa aatgtacgga tataagcata caatattctt tcaatgccag aaaacttatc
     9481 agcgcaaaaa acgccaacgt catccagcgt ggacctgaaa tcaccagtga aaaacaaatg
     9541 acgatgcccc accaggtacg aatacgtaat gtaacctcac ccccttttct ctccggtaac
     9601 cgccatagca gaaaacggtt tatcagcgtt gctaaaatta acaacgcga
//
LOCUS       DAZPJR010000156         9621 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPJR010000156 DAZPJR010000000
VERSION     DAZPJR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.160"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..2199
                     /locus_tag="ACHVB7_003493"
                     /pseudo
     CDS             1..2199
                     /locus_tag="ACHVB7_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2192..3328
                     /locus_tag="ACHVB7_003494"
     CDS             2192..3328
                     /locus_tag="ACHVB7_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1915386.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="ACHVB7_003495"
     CDS             3325..5325
                     /locus_tag="ACHVB7_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR1915387.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..5908
                     /locus_tag="ACHVB7_003496"
     CDS             5447..5908
                     /locus_tag="ACHVB7_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR1915388.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5949..6419)
                     /locus_tag="ACHVB7_003497"
     CDS             complement(5949..6419)
                     /locus_tag="ACHVB7_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1915389.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6466..7185)
                     /gene="btsR"
                     /locus_tag="ACHVB7_003498"
                     /gene_synonym="yehT"
     CDS             complement(6466..7185)
                     /gene="btsR"
                     /locus_tag="ACHVB7_003498"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR1915390.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="ACHVB7_003499"
     CDS             complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="ACHVB7_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR1915391.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9089..9154
                     /locus_tag="ACHVB7_003500"
                     /pseudo
     CDS             9089..9154
                     /locus_tag="ACHVB7_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9382..>9621
                     /locus_tag="ACHVB7_003501"
     CDS             9382..>9621
                     /locus_tag="ACHVB7_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1915392.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWF"
BASE COUNT         2354 a         2531 c         2468 g         2268 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     5881 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc atggccgcca
     5941 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     6001 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     6061 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat gtcatcggtt
     6121 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     6181 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     6241 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     6301 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     6361 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     6421 aggccttcag gtgttaatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     6481 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     6541 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     6601 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     6661 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     6721 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     6781 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     6841 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     6901 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     6961 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     7021 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     7081 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     7141 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     7201 ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     7261 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     7321 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     7381 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     7441 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     7501 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     7561 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     7621 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     7681 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     7741 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     7801 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     7861 aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca
     7921 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     7981 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     8041 ggcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc
     8101 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga
     8161 gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct
     8221 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
     8281 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggcgca
     8341 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
     8401 agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
     8461 ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca
     8521 acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag
     8581 ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
     8641 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
     8701 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
     8761 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
     8821 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
     8881 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
     8941 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
     9001 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
     9061 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
     9121 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttta aaaatcgacg
     9181 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
     9241 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
     9301 aaacaatatc aggaattgac actggttatc catacagata aaaataacac ctgtacataa
     9361 ccacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga aaaaatcact
     9421 aaaatgccaa acggtgctgt ggatgcgtta aaggaagaat taacccgacg catcagtaaa
     9481 cgttatgacg atgtagaggt gatcgtaaaa gccaccagca acgatggcct ttctgttaca
     9541 cgcaccgcag ataaggattc tgcaaaaaca tttgttcagg agactctgaa agatacctgg
     9601 gagtctgctg acgagtggtt t
//
LOCUS       DAZPJR010000157         9619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPJR010000157 DAZPJR010000000
VERSION     DAZPJR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.161"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..203)
                     /locus_tag="ACHVB7_003502"
     CDS             complement(<1..203)
                     /locus_tag="ACHVB7_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR1915393.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPAGNDSNLLIVFYVQI"
     gene            complement(203..1129)
                     /gene="glsB"
                     /locus_tag="ACHVB7_003503"
     CDS             complement(203..1129)
                     /gene="glsB"
                     /locus_tag="ACHVB7_003503"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR1915394.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1193..2581)
                     /gene="sad"
                     /locus_tag="ACHVB7_003504"
     CDS             complement(1193..2581)
                     /gene="sad"
                     /locus_tag="ACHVB7_003504"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR1915395.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2682..3563
                     /locus_tag="ACHVB7_003505"
     CDS             2682..3563
                     /locus_tag="ACHVB7_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1915396.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3640..4755
                     /gene="yneK"
                     /locus_tag="ACHVB7_003506"
     CDS             3640..4755
                     /gene="yneK"
                     /locus_tag="ACHVB7_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR1915397.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4905..6095
                     /gene="ydeA"
                     /locus_tag="ACHVB7_003507"
     CDS             4905..6095
                     /gene="ydeA"
                     /locus_tag="ACHVB7_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR1915398.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6120..6785)
                     /gene="marC"
                     /locus_tag="ACHVB7_003508"
     CDS             complement(6120..6785)
                     /gene="marC"
                     /locus_tag="ACHVB7_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR1915399.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6997..7431
                     /gene="marR"
                     /locus_tag="ACHVB7_003509"
     CDS             6997..7431
                     /gene="marR"
                     /locus_tag="ACHVB7_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR1915400.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7451..7834
                     /gene="marA"
                     /locus_tag="ACHVB7_003510"
     CDS             7451..7834
                     /gene="marA"
                     /locus_tag="ACHVB7_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR1915401.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7866..8084
                     /gene="marB"
                     /locus_tag="ACHVB7_003511"
     CDS             7866..8084
                     /gene="marB"
                     /locus_tag="ACHVB7_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR1915402.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(8141..9580)
                     /locus_tag="ACHVB7_003512"
     CDS             complement(8141..9580)
                     /locus_tag="ACHVB7_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1915403.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2485 a         2465 c         2167 g         2502 t
ORIGIN      
        1 attatctgaa cataaaacac tatcaataag ttggagtcat tacctgccgg agcaagacgt
       61 ttagcgacaa aatccgccgc atgttgttcg atgactggcg cgcctttctc caggcaatac
      121 tgacgctctt tcatgcccag acgaaagcga gagcgaaatt ttgaacggga taaacgcgcg
      181 aagagtggat cgagagactg cattaataaa ccgaacgtcc taactgtttc gtcaattgtt
      241 caaggacggc gatacccgca agcgagttac ctgcatcatc cagttccgga ctccagactg
      301 cgatggccat ttcatgcgga acaatcgcca caataccgcc accaacgcca gatttcgccg
      361 gtagtcccac ccgccaggca aactcccccg cgttctggta cataccactg gtcgccatca
      421 gcgcgttaat ttgccgcgcc tgcattggcg tcactacagg ttcatcaata tgaatagctt
      481 tcccctgatt agccagaaag acaaacgtcc gagccagctc tacacagctc attttcagag
      541 cgcagtaatg aaagtagttt tgcagaacgg ttgtcacgtc atgatggaaa ttgccaaacg
      601 acttcatcag ccaggcgata gccgcatttc gcgcggaatg ttcaaattcg gaacgcgcta
      661 ccaccgtatc gtatgaaata tcagacacac cgcttaagcc gcgcacgact tccagcatac
      721 gttgccgtgg tgcgcttaat cgcccttgca acatatcgca gaccaccagc gcaccagcat
      781 taataaacgg attacgcgga ataccctgtt ccatttccag ttgcactaaa gaattgaacg
      841 gtgatccaga cggatctttg ccgacgcgtt gccagatttc ctcttcggag taatgacgca
      901 tggcgacaac gagactcagc actttggaaa tagactgaat ggaaaaacgt tcttgcgcgt
      961 ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc aatgcccaat cgggaaccgt
     1021 ctactgtagc cagcgccgga atataatccg cgactttacc ctgaccaatg agcggccgca
     1081 cttgccgcaa gatgttttct aaaattgcat tatccatggc gactgccact ttctactcct
     1141 ggaccgcagg tctgaaaaga cctgcgagta tatcagagct gaatatgtcg cgtcagatcc
     1201 ggtctttcca caccgtctgg atattacaga attcgtgtaa gccgaaatgg gaaagctcac
     1261 gaccaaagcc actctttttc acgccaccaa aggccactcg cgcgtcgctg gcacaataac
     1321 cattgataaa caccccaccg cattccagac gtgccgccat ctgtctggcc tgtgtttcgt
     1381 cggtggtaaa aatggtcgct gaaaggccga actcactatc attagccagt tccagcgcat
     1441 gttctgcatc tttcgcaacg gtgattgccg caacggggcc aaacatttct tcccgaaacg
     1501 cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg atagtagtta cctgccccag
     1561 ccatcttttc cccgcccagt aacaaacgcg caccctgcgc cagggttttc tctacctgat
     1621 gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc gagagtgttc tcttcgtcac
     1681 ggggatcgcc cattttcaag gctgccgcag ctgccacaaa acgttcggta aatgcagaag
     1741 caattccctc ttcgataata aagcgttttg ccgctgcaca tacctgtccg gtattctgat
     1801 aacgtccggc taccgccgct ttaaccgcca gttccagatc ggcatcgtta agcacaataa
     1861 acggatccga accgcccagt tccagtacgc attttttcag tgccgctcca gcctgtgcgc
     1921 caatagccgc tccagcacga acacttccgg tcaccgtgac agcagcaatg cgcgagtctt
     1981 taatcatctg actgacaccg tcgttgtcgg cattcagcca gccatatacg ccttgcggga
     2041 ttcccgcatc tttaaacacc tgggcaatga gctgagcaca tcccatcaca ttcggcgcat
     2101 gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac agcgccacgc atcacctgcc
     2161 ataacggaaa attccacggc ataatcgcca gaatcgtccc caacggtcga tactcaataa
     2221 ccgcctgctg attttccacc agtgtaggtt ccgccttcag cattgccgga ccatgttctg
     2281 cataccagtc acacaaattc gccgatttcg ccacttcagc gcgcgcctgg ttgattggtt
     2341 tgcccatttc gcgggtgatc atttgcgcca tttcttcgct acgggcgcgc agagccttac
     2401 cgatatcacg cattttttca gcacgataat ctatatttgt ctcgcgccag tcgcgaaagc
     2461 ctgctgccgc cagctcaagt gcgttttcga tatcgttagc gccagcccac ggcagcacag
     2521 aaagttgttc acccgtggca ggatttattg aaattgcatg agttgccgga gtaatggtca
     2581 tcggggtatc tcctttatga gtcatggtat gaagatacgc agatttactc ttgctttaaa
     2641 atgaataata ttaagccact tattcacgaa tcgagaatgt tatggatctg acccaactgg
     2701 agatgttcaa cgcggttgcc gaagctggca gcattaccca ggctgcagca aaagtgcatc
     2761 gcgtaccgtc gaatctcact acccgtctac gccagctgga aacagaactg ggggtagatc
     2821 tgtttattcg cgagaatcag cgtttacgtc tctctcctgc cgggcataac tttttacgct
     2881 atagccaaca aattctcgcg ttagtggatg aagcgcggag cgttgtcgct ggcgatgagc
     2941 cgcaaggttt attttctctt ggttcgctgg aaagcaccgc agcagtgcgc attccggcca
     3001 cgctggcgga attcaaccat cgttatccca aaattcagtt ttcactttcc accggccctt
     3061 ccggcactat gctggaagga gtgctggagg gaaaactgaa tgcggcattt attgatgggc
     3121 ccattaacca tactgccatt gacgggatac cggtataccg cgaggagctg atgatcgtca
     3181 cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt taacggcagt aacatttatg
     3241 ccttccgcac caattgttcg tatcgtcgcc acttcgagag ctggtttcat gctgacggtg
     3301 ccgctccggg aactattcac gagatggagt cgtatcacgg gatgttggcc tgtgtggtcg
     3361 caggagcagg cattgcgctt attccacgct ctatgctgga aagtatgccg gggcatcacc
     3421 aggttgaagc gtggccgtta gctgagcaat ggcgttggtt aacaacctgg ctggtctggc
     3481 gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca actactggat gtgtctgact
     3541 cggcaaaaca gagttaccaa tgaactattt tgataattct tgcggctaat atgctctata
     3601 tagtgatgtt ccgatgactt atgactatat ggggcaaata tggttacgcc agtaagcatc
     3661 agcaattaca tatctcttcc tgatgatttt cccgttcgta atattgcacc acaggtaaaa
     3721 gaagttttaa aggactttat tgatgcgcta agtacaataa tatgtgatga agagtggcgc
     3781 acgtctttaa acatcaactc agccacaaaa aagatattta ataaccttga taacttatca
     3841 tatattcaga ggacttcttt ccggggtaac gacacgctat acaatgaaaa ggttcagttt
     3901 aaactcactt atcccgtcaa gaatggaaga cacaaagaaa atattgaatt tcaggtagta
     3961 ataaatttaa gccctattta tttagataat tttcgccatg atggagaaat taatattttt
     4021 tgcgctccca acccaaagcc tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt
     4081 gtgctctttc tgtttctgaa tgattttatt gaacaatttc caatgttcaa ccctggcgtt
     4141 ccgatcaaaa tagcgcatac accacatatt gaacccctgc ctccggatca ccataccgct
     4201 gcagattact tacgccagtt tgatttgctt gtcctgaatt ttatctctcg cggtaatttt
     4261 gtcatactcc cccgattatg gaataactct gaggttcaca gatggttcgt caataaagat
     4321 cctaacttga tcaccgccat tctcgacata acggacagcg aattaaaaga ggatttattg
     4381 caaagcctga tggattcatt aggttctaac aaacatgtac tacccgaagt ctgcatctgc
     4441 tttttattcc ttttagcaga acaagaatct cctcattttc aggacttgtt tttatttttc
     4501 gccaatatgt tattgcacta tcaccaattt atgaatccca atgaaagtga tttgaatgac
     4561 gtgttgatgc cagcatcatt aagtgatgat aaaattatca agcatatggc gcgcaggacc
     4621 ctcgaactgt ttgtaaaaaa tgaaacaccg ccaaaagtta ctcacgaaga cctggtgaaa
     4681 aacaggcctc gctcccctgt cagaccacct atacccgcaa cctccaaaac gccagacctc
     4741 cctgaacgtc attaaaccgt gatgttaccg actctctgac gcggaacaga atcagcgtca
     4801 gagaaacgga aaacgcgatc cagatcacaa atacattgta ttcacatcat taaccgtttt
     4861 aagatcattt catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg
     4921 tttcccgcaa agtggcgtgg ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca
     4981 acaccaccga atttgtccct gttggcctgc tctctgacat tgcgcaaagt tttcacatgc
     5041 aaaccgctca ggtcggcatc atgttgacca tttacgcatg ggtagtagcg ctaatgtcat
     5101 tgccttttat gttaatgacc agtcaggttg aacggcgcaa attactgatc tgcctgtttg
     5161 tggtgtttat tgccagccac gtactgtcgt ttttatcgtg gagctttacc gttctggtga
     5221 tcagtcgcat tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtctctgg
     5281 cgatccgtat ggctccggcc gggaagcggg cacaggcctt gagtttaatt gccaccggta
     5341 cagcactggc gatggtctta ggtttacctc tcgggcgcat tgtggggcag tatttcggtt
     5401 ggcgaatgac cttcttcgcg attggtattg gggcgcttat cacccttttg tgcctgatta
     5461 agttacttcc cttactgccc agtgagcatt ccggttcatt gaaaagcctc ccgctattgt
     5521 tccgccgccc ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt
     5581 acacggcata cagctatatc gagccttttg tgcaaaacat tgcgggattc agcgccaact
     5641 ttgccacggc attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg
     5701 gtaaactggg taatcagtat gcgtctgcgt tggtgagtac ggcgattgcg ctgttgctgg
     5761 tgtgtctggc actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta
     5821 ttttctgggg gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc
     5881 tggcaccaga tgctaccgac gtcgcgatgg cgctattctc cggcatattt aatattggaa
     5941 tcggggcggg tgcgttggta ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg
     6001 gttatgtggg cgcggtgcct gcttttgccg cgttaatatg gtcaatcatt atatttcgcc
     6061 gctggccagt gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt
     6121 taatggtacg ttttaatgat ttccaggatg ccgttaataa taaactgcac acccatacat
     6181 accagcagga atcccatcag acgggagatc gcttcaatgc cacccttgcc caccagccgc
     6241 ataattgcgc cggagctgcg taggcttccc cacaaaataa ccgccaccag gaaaaagatc
     6301 agcggcggcg caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggac
     6361 gccgagctaa taatcatcgc tatggttccc ggaccagcgg tacttggcat tgctagcggc
     6421 acaaaggcaa tattggcact gggttcatct tccagctctt ccgacttgct tttcgcctcc
     6481 ggtgaatcaa tcgctttctg ttgcggaaag agcatccgaa aaccgataaa cgcgacgatt
     6541 aagccgcctg caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc
     6601 ccggcgtaat acgccaccat catgatggca aatacgtaca ccgaggccat caacgactga
     6661 cgattacgtt cggcactgtt catgttgcct gccaggccaa gaaatagcgc gacagttgtt
     6721 aatgggttag ctaacggcag caacaccacc agccccaggc caattgcttt aaacaaatct
     6781 aacattggtg gttgttatcc tgtgtatctg ggttatcagc gaaaagtata aggggtaaac
     6841 aaggataaag tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat
     6901 ttagcaaaac gtggcatcgg tcaattcatt catttgactt atacttgcct gggcaatatt
     6961 atcctctgca actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc
     7021 aatgaaatta ttccattggg tcgcttaatc catatggtta atcagaagaa agatcgtctg
     7081 cttaacgagt atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct
     7141 atccgctgcg cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg
     7201 ggagcactga cccgtatgct ggatcgcctg gtctgtaaag gctgggttga aaggttgccg
     7261 aacccgaatg ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgc
     7321 gaacaatgcc atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg
     7381 gcggacgaag ttgcaacact tgagcatttg cttaagaaag tcctgccgta aacaaaaaag
     7441 aggtatgacg atgtccagac gcaatactga cgctattacc attcatagca ttttggactg
     7501 gatcgaggac aacctggaat cgccactgtc actggagaaa gtgtcagagc gttcgggtta
     7561 ctccaaatgg cacctgcaac ggatgtttaa aaaagaaacc ggtcattcat taggccaata
     7621 catccgcagc cgtaagatga cggaaatcgc gcaaaagctg aaggaaagta acgagccgat
     7681 actctatctg gcagaacgat atggcttcga gtcgcaacaa actctgaccc gaaccttcaa
     7741 aaattacttt gatgttccgc cgcataaata ccggatgacc aatatgcagg gcgaatcgcg
     7801 ctttttacat ccattaaatc attacaatag ctagttgata acgtgacaac gtcactgagg
     7861 caatcatgaa accactttca tccgcaatag cagctgcgct tattctcttt tccgcgcagg
     7921 gcgttgcgga acaaaccacg cagccagttg ttacttcttg tgccaatgtc gtggttgttc
     7981 ccccatcgca ggaacaacca ccgtttgatt taaatcacat gggtactggc agtgataagt
     8041 cggatgcgct cggcgtgccc tattataatc aacacgctat gtagttattg tcaggctctg
     8101 atcacagagc ctgacatctc cgtaatatct tcctcaagtc tcaaatattt tcgccattgt
     8161 tggcgatgag atcccgatac cagtaaaacg acgctttacg tatccgttcc agcgttccct
     8221 tcccttcgtt gtctttatcg acataaatca ttccgtagcg ctttttcatt tcccctgttc
     8281 cggcagaaac caggtcaatg cagccccacg gggtgtagcc aattaagtca acaccatctt
     8341 caactacggc ttttttcatt tcccgaatat gggaagcaaa gtaatcaatg cgatagtgat
     8401 cgttcaccgt gccgtcagct tgtctctggt caaccgcacc aaatccattt tcgacaataa
     8461 acagcggcaa ctggaaatga tcccagaacc agttgagtga ataacgtagc cctgccggat
     8521 caatttgcca tccccattcg gatgtatcga tataagggtt gctgaccaaa tctcgtggtt
     8581 caacataatc cagttgcggg ttatcgggag aaaattgtgt cgtaaaagac atgtagtagc
     8641 taaaaccgat aaagtcgaca catccactgg caagaatcgc gttatcttct ggtgtgatat
     8701 cgaggttgaa tcctttcctg gcaaagtaat tcagcatatg ttgcggataa tatccacgag
     8761 catgaacatc agtaaaccag taacgacgat gcatcgcttt cgtggccatc atcatatcgt
     8821 tgggtgcaca cgtcagagga tagatggggc acatggcgat catacagcca atgttaaatt
     8881 caggattgat cgattttcca gtctgtaccg ccagggcact ggcaactaac tcgtaatgta
     8941 ccgcctggta cattatttgt tcgcgctcat tgatatcttc ctctggcgaa tacaagatac
     9001 cggaattagt aaatggacac aggctttcgc tgaaattcac ctgattattg atttcgttaa
     9061 acgtcatcca gtactttact ttttctttat agcgcgtgaa gaccgttgat gcgaagcgga
     9121 taaaaaagtc gatcagctta cggtttcgcc agccaccata ttttgtcacc agatgataag
     9181 gcatctcaaa atgcgaaagc gtcaccacag gttccattcc ttgcttcagg cattcatcga
     9241 acagatcatc ataaaattgt aaaccctctt cattcggctc ctgttcgtca ccttgcggaa
     9301 agattcgtgt ccaggcaatg gaagttcgaa agcatttgaa tcccatctcg gcaaataact
     9361 gaatatctgt tttatagcga tgataaaaat caattgcttc atgattggga taattaagcc
     9421 cgtcgataac gccttctgtc acttcacgcg gcaccccgtg agcgccagca gtcatcacat
     9481 cagcgatact gatgcctttt cctccttcat tccagccacc ttccaactga tgcgcggcta
     9541 ccgcgccacc ccataaaaaa ccttttttaa atcctgacat acacaactcc ttaactaaaa
     9601 tagattacca ccagacttc
//
LOCUS       DAZPJR010000158         9614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPJR010000158 DAZPJR010000000
VERSION     DAZPJR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.162"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..154
                     /locus_tag="ACHVB7_003513"
     CDS             <1..154
                     /locus_tag="ACHVB7_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR1915404.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="ACHVB7_003514"
     CDS             258..1172
                     /locus_tag="ACHVB7_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1915405.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAADGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="ACHVB7_003515"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="ACHVB7_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR1915406.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="ACHVB7_003516"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="ACHVB7_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR1915407.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="ACHVB7_003517"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="ACHVB7_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR1915408.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="ACHVB7_003518"
     CDS             5467..7149
                     /locus_tag="ACHVB7_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1915409.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="ACHVB7_003519"
     CDS             7201..8358
                     /locus_tag="ACHVB7_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1915410.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="ACHVB7_003520"
     CDS             complement(8324..8446)
                     /locus_tag="ACHVB7_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915411.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8661..>9614
                     /locus_tag="ACHVB7_003521"
     CDS             8661..>9614
                     /locus_tag="ACHVB7_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HFR1915412.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAF"
BASE COUNT         2815 a         2045 c         2047 g         2707 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cgatggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cataaccact gaaaattaaa aatttctata tatttcatat
     8581 tgttaaatga tacttaaata aatttcatca gtaaatcatt gccatagata atcattggat
     8641 ggcacaatca attttcgcta atgataacca ttcccattac gttttatatg ttgatagcaa
     8701 agtatctctg tctgctgaag cccttttggc tgcgtaagaa taataaaact tcagtgttgt
     8761 taatcattat tatcctcgca atgatccttg gcgtggttaa aattcaggtc tggttaaacg
     8821 actggaataa tgattttttc aatgcgctaa gccagaaaga aaccgataag ctttggcagc
     8881 ttgttctttg gttccctgcc ctgctgggga tctttgtatt aatatccgta aacaaaacat
     8941 ggttaattaa actactgact attcgctggc gcgaatggtt aacagattac taccttaatc
     9001 gctggtttgc agataaaaac tactacttca cgcagatcta cggcgagcat aaaaatacag
     9061 ataaccctga ccaacgtatc gctgaagaca ttctcttact gatcagcaaa acgctcagtc
     9121 tttcatttgg cttcatccag tcacttagca tgctgatcac ctttaccgtt attctttggc
     9181 aaagcgcggg tacgctctca ttcactgttg gtggaacaga atggaatatc caggggtata
     9241 tggtctatac cgtcgtgctc attgtgatcg gtggaacttt atttacccat aaggtgggta
     9301 aacgtattcg tccgcttaat gtggaaaaac aacgtagcga agcgacattt cgaactaatc
     9361 ttgtgcagca taataagcag acagaactga ttgcgctaag caatgctgag tctctacaac
     9421 gccaggagtt gcgcgacaat tttcatacca ttaaagagaa ctggcatcgt ttaatgaatc
     9481 gtcagcggtg gcttgattac tggcaaaata tttattcgcg ctcgcttagc gttcttccct
     9541 actttctgtt attgccgcag tttattagcg ggcagatcaa tctgggcgga ctgatgaaat
     9601 cgcgccaggc attt
//
LOCUS       DAZPJR010000159         9542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPJR010000159 DAZPJR010000000
VERSION     DAZPJR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.163"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1321
                     /locus_tag="ACHVB7_003522"
     CDS             <1..1321
                     /locus_tag="ACHVB7_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1915413.1"
                     /translation="PQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHY
                     VLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCAR
                     AALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTLRV
                     MSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDH
                     NGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNPAE
                     IYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGFQN
                     RFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRVRV
                     RLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1324..2652
                     /gene="ssnA"
                     /locus_tag="ACHVB7_003523"
     CDS             1324..2652
                     /gene="ssnA"
                     /locus_tag="ACHVB7_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR1915414.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2703..3482
                     /gene="ygfM"
                     /locus_tag="ACHVB7_003524"
     CDS             2703..3482
                     /gene="ygfM"
                     /locus_tag="ACHVB7_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR1915415.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3479..6418
                     /locus_tag="ACHVB7_003525"
     CDS             3479..6418
                     /locus_tag="ACHVB7_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR1915416.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6351..6665
                     /locus_tag="ACHVB7_003526"
     CDS             6351..6665
                     /locus_tag="ACHVB7_003526"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR1915417.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6701..7051
                     /gene="ghxQ"
                     /locus_tag="ACHVB7_003527"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6701..7051
                     /gene="ghxQ"
                     /locus_tag="ACHVB7_003527"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7120..7608)
                     /locus_tag="ACHVB7_003528"
     CDS             complement(7120..7608)
                     /locus_tag="ACHVB7_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1915418.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="ACHVB7_003529"
     CDS             complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="ACHVB7_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR1915419.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2301 a         2559 c         2488 g         2194 t
ORIGIN      
        1 tcctcagttc cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca
       61 cggcgtgaaa tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca
      121 gggcttccac tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc
      181 gggcgacaac caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac
      241 agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg
      301 cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc
      361 actacaagag atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga
      421 gttccgtttc ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt
      481 tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac
      541 agtaacactg catgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc
      601 cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg
      661 cgaaactcgt ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat
      721 tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat
      781 ccgttcccac cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg
      841 taaaggcgat atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca
      901 ggaagccgct cgctgcctcg aatgtaacta cgtttgcaac aagtgtgtgg atgtctgccc
      961 gaaccgcgcc aacgtctcca ttgcagtccc tggcttccag aatagattcc agacgctgca
     1021 cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa
     1081 accatacgaa gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcac
     1141 cccaggcttc cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgggt
     1201 gttaaacatc gacagcgaag gtcagtttaa caacgtacca ccggagctga acgatatgtg
     1261 ccgcatcatc agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta
     1321 atcatgttga ttctgaagaa tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa
     1381 ggcgttgata tcgctatcga aaatgacgtg attgtcgcta tcggcgatgc cctgacgcaa
     1441 cgctaccccg atgccagcta caaagagatg catggtcgga ttgtgatgcc gggaattgtc
     1501 tgctcgcaca accattttta ctcggagctt tcccgcggaa ttatggcaaa catcgcccct
     1561 tgcccggatt tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat
     1621 gaagagtcgc tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt
     1681 acatcggtta tcgatcacca tgcctcgccg gcgtatatcg gcgggtcgct ctccacattg
     1741 cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt
     1801 aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg
     1861 attgatgagg cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct
     1921 cacgcgccgt ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc
     1981 acaggccgtg gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac
     2041 cactggtacg gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa
     2101 acgctggtcg ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc
     2161 gatgcgttcc tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat
     2221 caccttagcg acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt
     2281 gaagagatga aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac
     2341 agttttgcca aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa
     2401 tttgggcttc tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg
     2461 acgccgctgc tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc
     2521 gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat
     2581 tgcgattcca tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat
     2641 gcgctggcat aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga
     2701 agatgattga acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc
     2761 gctaccagga tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc
     2821 gtaccgataa aaagattgcc atttccttac aggatctgga actggactgg gttgactggg
     2881 ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca gccactgcgt gatgcgcgct
     2941 ttattcctgc agcactgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc
     3001 agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga gtcagtgctg cttcccgtcc
     3061 tgctagcact ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact
     3121 acctggcctg cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca
     3181 cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg
     3241 ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac
     3301 tgcgtctgca cgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg
     3361 ccaacgccat tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca
     3421 cgggagttct ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat
     3481 gatcatccac tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa
     3541 cgtgcaaaag ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggctt
     3601 cgggtttgcc ggttctgacg caataatctt taacggtaat atcgttaacg cgtccttgct
     3661 tattgccgca cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa
     3721 cgagttaagt ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa
     3781 cgatccagct gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg
     3841 cgaagagatc gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata
     3901 ctatcaggtc attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat
     3961 cgctccgact ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc
     4021 cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac tgcgtaacgg ctgacgcctg
     4081 cgtcattaaa atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag
     4141 caaagctgaa gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat
     4201 ctactatacc ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt
     4261 cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga
     4321 aattgcgctc gaagcactga agctcatcga cgttgaatat gaagtgctta agccggtaat
     4381 gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta
     4441 tgttgctggt gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga
     4501 gcatatgatc atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat
     4561 tcatggtcat attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg
     4621 aacctataac tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg
     4681 gatggacggc gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg
     4741 ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt
     4801 tggcggcggt tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac
     4861 ctgcgtgacc gggcgtccgg tactattccg ctacacccgt gaagaagagt ttattgctaa
     4921 cacctctcgt cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct
     4981 gacggcagtg aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac
     5041 cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt
     5101 ccaggtcacc acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc
     5161 accgaaaggt aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat
     5221 cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct
     5281 cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact
     5341 ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa
     5401 tggtgactgg cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga
     5461 tatcgatcag gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc
     5521 tggcggtgcg gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt
     5581 gctgcactgc ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt
     5641 tgataaaggc gcatatgcct cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc
     5701 agcggaaaat ctacgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt
     5761 ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt
     5821 cggggatatt gcccataaag gcgaaaccgg catcggcttt ggttcactgg tgggaactgg
     5881 cagttatatc acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt
     5941 caacacgcgt acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac
     6001 accggtcaac ccggaattag cattgggaca aatctacggt gctaccctgc gagctatcgg
     6061 ccacagtatg agcgaagaga tcatttatga cgccgaaggt cacccgttaa cgcgtgattt
     6121 acgcagttac ggcgcaccga aaattggtga cattccgcgt gatttccgcg ctgtgctggt
     6181 gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg atctcggaaa tcggtgtaaa
     6241 tggcgcagct ccggcgattg ctaccgcaat tcacgataca tgcggcatct gggcagaaga
     6301 aagtcaaagt gggcatggga acggatatcg gtgccggaac aactttcaac atgctgcaaa
     6361 cgctgaacga agcctacaaa gtgttgcaat tacaaggcta tcgcctctcg gcatatgaag
     6421 cgttttacct ggccacgctc ggcggagcga aatctctggg ccttgacgat ttgattggca
     6481 actttttacc tggcaaagag gctgatttcg tggtgatgga acccaccgcc actccgctac
     6541 agcagctgcg ctatgacaac tctgtttctt tagtcgacaa attgttcgtg atgatgacgt
     6601 tgggcgatga ccgttcgatc taccgcacct acgttgatgg tcgtctggtg tacgaacgca
     6661 actaataata aaactttaac atcctcgtga ggacatcatt atgtctggag acatcctaca
     6721 aacaccggac gcaccaaagc cacagggcgc gctggataat tattttaaaa ttaccgctcg
     6781 tggcagtacc gttcgtcagg aagtactggc tggcttaacg acctttctgg ccatggttta
     6841 ttccgttatc gtcgttccgg gaatgctggg caaagcagtt tttcctcccg cagctgtgtt
     6901 tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg atggggttgt aggccaattt
     6961 gccaatggcg attggttgtg cgatttcctt gacggcattt accgcattca gtctggtact
     7021 cgggcaacaa attagcgttc ctgtcgcact ggtcgcattt tacgcgggtg gttgggcaat
     7081 ctaatagatt ctccgcgcct ttcagcgcgg agatttcttt cacagaggat tctcaccgct
     7141 ggcaacaata cgctgctggc ggactcgcct gagttctctt tcagtaagca actgcagcgc
     7201 ctgtgtcggg caagattcaa cacaggctgg cccctcttcc cgctgttcac agagatcgca
     7261 tttcacaatt tgttgtcggg tatcccccgg caacgactga atggtgatca ttccaaacgg
     7321 gcacgcactg acgcaactct gacagccaat acaacgggca gaattggtct gcaccacctg
     7381 ctcgcccatc gtcagcgccc ccacggggca agcgccaaca caaggggcgt tttcacactg
     7441 atggcacatc accggcgcac tgatgctgtc cagtcgctgt accttcagcc ggggtaaaaa
     7501 gacatcggca ttcaactcct gttctgaagg gtgagcgacc acacaggcta cttcacaagt
     7561 acggcagcca atacagtcag ccggattaac gataattaac gatttcatca cgatgccttc
     7621 gtatcaaaca gagttaacat atcgcgcgcc gcctgccttc ctgcggccat tgcagtgaca
     7681 accagatccg cgccatgaac tgcatcacca ccagcaaaga cttttttcag atgcgtctgg
     7741 gtaggtaaat acccgacgtc accggtttga atcaggcccc atttatcgag tttaattccg
     7801 ctgccctgca accacggcat ggcatgcgcc tggaaaccaa aggccataat cagaacatcg
     7861 gcgggcaatt caaactctga acccgctacc ggacgaggac gacggcgacc atccggcccc
     7921 ggctcaccca tggcggtacg aatcaggccc accgcagtta aacgcccatc ttcgtcacaa
     7981 gcgatatatt gcggttgaac attgaactga aactcgacac cttcctcgcg cgcattaacc
     8041 acctctttgc gcgagcccgg catactgact tcatcacgac gatacgcgca ggtcacgctg
     8101 gcagcattga ggcgaatgga agtccgcaaa caatccattg tcgtatcgcc accgcccaat
     8161 accacgactc gcttaccttc cacgtccgtc agcggatact cttcagactc cggcaatccc
     8221 atgagctggc gggtatgggc agtcaggaac ggtagagcct gaatgacacc gggcgcatct
     8281 tcatgcggca gatctgctcg catcatcccg taagtcccca cgccgatgaa aactgcatca
     8341 tattcagaag ttaaatcgct aaaggtaata tcgcggccaa tttcacagtt aagatgaaag
     8401 tcgattccca ttgcggtgaa tatctctcgc cgctggctta ataccgtttt atcgagtttg
     8461 aaaggaggaa tgccaaaagt cagcataccg ccaatttctg gatggcgatc aaagacatca
     8521 acctgaactc ccgcgcgcgc cagaatatca gcacacccta accctgctgg cccagcgcca
     8581 atcaccgcca ctttttcgat acggggaaca actttgctga catcgggacg ccagcccatc
     8641 gccagcgcgg tatcggtgat gtagcgttcc agattaccga tagagactgc gccagagtga
     8701 tctttcaaag tacatgcgcc ttcacaaaga cggtcctgtg ggcataccct gccgcagatt
     8761 tctggtaagg aactggtctg gtggcaaagt tctgccgctt caataatctt tccttcctgt
     8821 accagacgga tgtaatccgg aatagcgtta tgcagcgggc aatgccagtt gcagttagct
     8881 ttttcggcac aataaacaca gcggtcactc tcataagtcg cttgcagtgg atccagcccg
     8941 caatagattt cgccaaagtg ggttttccgt tcactcgctg aaattttatc tgcgccttta
     9001 cgcgagttaa cggggagcaa cgctgcactg cgagatggct gagcgtctga tgacgctttt
     9061 cctgctgccg ttttgcgctg gcgagccacc tttatccact gtaacccttt atcgtccatc
     9121 agtcgtaacg cctgtgttgg gcagacttca atacaggctt gcgtgccgga actgcgctgg
     9181 ttacaaaggt cgcatttctg cgcaatcgta tcgaccatct caacgacgcc aaaggggcaa
     9241 gcgattgcgc atcttttaca gccaatacat ttttgctcgt ccagttgtac gctatcggac
     9301 tggaaagtca gagcattaac cggacaagcc gtaacacaag gggcattgtt gcagtgatgg
     9361 caggccaccg gattcgcagc ctggcctttc cctacaacgt ggatacgcgg tcgaaagtca
     9421 ctgtgactca gcggccagtt ttcttgattg tgtgccaccg cacaggcaat ttcacaagca
     9481 tgacagccta tacattccgc agcttcagca gcgataaact tattcatttg catccctttc
     9541 at
//
LOCUS       DAZPJR010000160         9501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPJR010000160 DAZPJR010000000
VERSION     DAZPJR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.164"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHVB7_003530"
     CDS             <1..108
                     /locus_tag="ACHVB7_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1915420.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHVB7_003531"
     CDS             complement(149..1231)
                     /locus_tag="ACHVB7_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR1915421.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHVB7_003532"
     CDS             complement(1228..3060)
                     /locus_tag="ACHVB7_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR1915422.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHVB7_003533"
     CDS             complement(3275..3631)
                     /locus_tag="ACHVB7_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915423.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHVB7_003534"
     CDS             complement(3689..4111)
                     /locus_tag="ACHVB7_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1915424.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHVB7_003535"
     CDS             complement(4127..4852)
                     /locus_tag="ACHVB7_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR1915425.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHVB7_003536"
     CDS             complement(4874..5620)
                     /locus_tag="ACHVB7_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1915426.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHVB7_003537"
     CDS             complement(5627..6691)
                     /locus_tag="ACHVB7_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR1915427.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGGPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHVB7_003538"
     CDS             complement(6763..7188)
                     /locus_tag="ACHVB7_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1915428.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHVB7_003539"
     CDS             complement(7185..7448)
                     /locus_tag="ACHVB7_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1915429.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHVB7_003540"
     CDS             7560..8060
                     /locus_tag="ACHVB7_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1915430.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHVB7_003541"
     CDS             8080..8274
                     /locus_tag="ACHVB7_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR1915431.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHVB7_003542"
     CDS             8274..8564
                     /locus_tag="ACHVB7_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1915432.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="ACHVB7_003543"
     CDS             8844..8996
                     /locus_tag="ACHVB7_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1915433.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9008..9373
                     /locus_tag="ACHVB7_003544"
     CDS             9008..9373
                     /locus_tag="ACHVB7_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915434.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2443 a         2494 c         2016 g         2548 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggcc ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatac tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac c
//
LOCUS       DAZPJR010000161         9432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPJR010000161 DAZPJR010000000
VERSION     DAZPJR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.165"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="ACHVB7_003545"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="ACHVB7_003545"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR1915435.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHVB7_003546"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHVB7_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR1915436.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="ACHVB7_003547"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="ACHVB7_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHVB7_003548"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHVB7_003548"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR1915437.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHVB7_003549"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHVB7_003549"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR1915438.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHVB7_003550"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHVB7_003550"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR1915439.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="ACHVB7_003551"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="ACHVB7_003551"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR1915440.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="ACHVB7_003552"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="ACHVB7_003552"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR1915441.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="ACHVB7_003553"
     CDS             complement(6556..7449)
                     /locus_tag="ACHVB7_003553"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1915442.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="ACHVB7_003554"
     CDS             complement(7446..8099)
                     /locus_tag="ACHVB7_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR1915443.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="ACHVB7_003555"
     CDS             complement(8110..9369)
                     /locus_tag="ACHVB7_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR1915444.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2159 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAZPJR010000162         9397 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPJR010000162 DAZPJR010000000
VERSION     DAZPJR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.166"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            197..301
                     /locus_tag="ACHVB7_003556"
     CDS             197..301
                     /locus_tag="ACHVB7_003556"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR1915445.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(350..1603)
                     /locus_tag="ACHVB7_003557"
     CDS             complement(350..1603)
                     /locus_tag="ACHVB7_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1915446.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="ACHVB7_003558"
     CDS             1710..2603
                     /locus_tag="ACHVB7_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1915447.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="ACHVB7_003559"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="ACHVB7_003559"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR1915448.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="ACHVB7_003560"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="ACHVB7_003560"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1915449.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHVB7_003561"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHVB7_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR1915450.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHVB7_003562"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHVB7_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR1915451.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHVB7_003563"
     CDS             complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHVB7_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR1915452.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHVB7_003564"
     CDS             complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHVB7_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR1915453.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="ACHVB7_003565"
     CDS             complement(8323..>9397)
                     /locus_tag="ACHVB7_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR1915454.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2379 c         2438 g         2131 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DAZPJR010000163         9212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPJR010000163 DAZPJR010000000
VERSION     DAZPJR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.167"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            73..732
                     /locus_tag="ACHVB7_003566"
     CDS             73..732
                     /locus_tag="ACHVB7_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR1915455.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="ACHVB7_003567"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="ACHVB7_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR1915456.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="ACHVB7_003568"
     CDS             complement(1860..2570)
                     /locus_tag="ACHVB7_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR1915457.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="ACHVB7_003569"
     CDS             3004..3294
                     /locus_tag="ACHVB7_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR1915458.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="ACHVB7_003570"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="ACHVB7_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR1915459.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            3927..3998
                     /locus_tag="ACHVB7_003571"
     CDS             3927..3998
                     /locus_tag="ACHVB7_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915460.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHVB7_003572"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHVB7_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR1915461.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="ACHVB7_003573"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="ACHVB7_003573"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR1915462.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="ACHVB7_003574"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="ACHVB7_003574"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR1915463.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="ACHVB7_003575"
     CDS             complement(7538..7837)
                     /locus_tag="ACHVB7_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR1915464.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="ACHVB7_003576"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="ACHVB7_003576"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR1915465.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9212)
                     /locus_tag="ACHVB7_003577"
     CDS             complement(9049..>9212)
                     /locus_tag="ACHVB7_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR1915466.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         2286 a         2328 c         2268 g         2330 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc ta
//
LOCUS       DAZPJR010000164         9188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPJR010000164 DAZPJR010000000
VERSION     DAZPJR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.168"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHVB7_003578"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHVB7_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHVB7_003579"
     CDS             complement(1751..2509)
                     /locus_tag="ACHVB7_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR1915467.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHVB7_003580"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHVB7_003580"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR1915468.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHVB7_003581"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHVB7_003581"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR1915469.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHVB7_003582"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHVB7_003582"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR1915470.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHVB7_003583"
     CDS             complement(5474..6079)
                     /locus_tag="ACHVB7_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915471.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="ACHVB7_003584"
     CDS             complement(6082..6849)
                     /locus_tag="ACHVB7_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915472.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="ACHVB7_003585"
     CDS             complement(6852..7562)
                     /locus_tag="ACHVB7_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915473.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="ACHVB7_003586"
     CDS             complement(7578..9083)
                     /locus_tag="ACHVB7_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1915474.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2358 c         2262 g         2196 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctttgg
//
LOCUS       DAZPJR010000165         9072 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPJR010000165 DAZPJR010000000
VERSION     DAZPJR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.169"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(506..1273)
                     /gene="fecE"
                     /locus_tag="ACHVB7_003587"
     CDS             complement(506..1273)
                     /gene="fecE"
                     /locus_tag="ACHVB7_003587"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR1915475.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="ACHVB7_003588"
     CDS             complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="ACHVB7_003588"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR1915476.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="ACHVB7_003589"
     CDS             complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="ACHVB7_003589"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR1915477.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="ACHVB7_003590"
     CDS             complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="ACHVB7_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR1915478.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="ACHVB7_003591"
     CDS             complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="ACHVB7_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR1915479.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="ACHVB7_003592"
     CDS             complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="ACHVB7_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR1915480.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="ACHVB7_003593"
     CDS             complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="ACHVB7_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR1915481.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8188..8256
                     /gene="ykiE"
                     /locus_tag="ACHVB7_003594"
     CDS             8188..8256
                     /gene="ykiE"
                     /locus_tag="ACHVB7_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR1915482.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8343..9040
                     /locus_tag="ACHVB7_003595"
                     /pseudo
     CDS             8343..9040
                     /locus_tag="ACHVB7_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1987 a         2508 c         2585 g         1992 t
ORIGIN      
        1 cactttttag aaaaatcgat ggtcagaaac tggattagca attccgttcc agggttgctt
       61 ttgatttacg ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat
      121 gaaccagctc cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tcccccccct
      181 gaaatctaaa cttagtcatg tcacgttttt gggtttctaa aattttaact tcgcgttttt
      241 cgttgccgta agggttatac agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg
      301 atcacgactg gtttgaaagc cgcgcccaag cctgtacagc tctggtttgc gttgattatg
      361 aacctgtcag cctaaagcaa gcggatggac gatgagtatt ggtaatcttt cagagtccgg
      421 aaaagttcag ccccagtctg aacaggcttg ctggcgccag tccagtttca ttcagtcgtg
      481 gtttggttct tacggcctgt gcaatctacc tcattaggca catcggcctg ccagataccg
      541 gctcggggtg tatttccgct tccacgctga atactgttct cagcaatcct ggggtcatca
      601 cctcttctgg tgtgccttgc gccataacat gtccgtttgc cattaccacc agttgatcgc
      661 agtaccggct agcctgatta aggtcgtgca gcacagcgac caccgttttc ccctgagtcc
      721 ggagttcgcc catcaaccgc atcaggtcca cctggtgatt gatatcaaga taggtggttg
      781 gctcatcaag taatacaacg ggcgtattct gggccaggac cattgccaga aatgcgcgct
      841 ggcgctgacc gccggaaagc tcggttaacc gacgaacggc aagatgattg atccgggtct
      901 ggttcatggc gacattaact cgtgcattgt cttcagcgga gagacgcccc cagagtgaca
      961 gccagggatt acgaccatac gaaaccagct cctggactgt gatcccctct ggcgttaaat
     1021 ggtgctgagg cagcagcgaa agcctgcggg ccaactggcg cgatgagagc atatttatgg
     1081 gattatcgcc gagaaatacg gtgccagact gcggcattaa aagccgcgaa aaacagttta
     1141 acagcgtcga tttcccgcaa ccgttaggac cgatcagggc ggtgatcttc cccgttggca
     1201 gtgagagtga aacgtcgtta agtaccttgt ctgttccgta attgaccgtc agattttcag
     1261 ttcgtaaagt catttatcgc attctcacaa gcaaccagac aaaccacggc gcaccgataa
     1321 tggcggtcag cacgccaacc gggagctcca gtgggggatg aataattctc gccagcagat
     1381 cggcaaccac caacagcaac gcacctgtca gggccgaaac aggcagcagt ctgcggtgac
     1441 gtccaccggt gatgctacgc atcatatgcg gcaccacgag accaataaag ctaatcgggc
     1501 cgcaggcggc cacgccggta gatgtcatgg cgacagctag taacaaagcc cagaatcggg
     1561 tatagggcac cgacacaccg agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga
     1621 gatcgcggca aaaactcagg ctcagcggca gaaataaaat catcagcggg atggcaatct
     1681 tcacaaagct ccagtcacgg ccccataagc tgccggtcag ccacagcagg gcgttgttca
     1741 catcctgcgg gcgcgagagc atcagataat ccgtcaggct ggcccagcat gcagaaagcg
     1801 ccacgccggt gagtgccagc ttcatcggct ggtgggtctt tgccagcatc ttcagcaata
     1861 tcaaccccgc catgccgccc gcaaaggcca gcagcggcag caccatcacg ggcagtgacg
     1921 gcataagaag tagagccccc acagaggcca ggctggcggc atggttaacg ccgagaatat
     1981 ccggtgatgc cagagggttg cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg
     2041 ctgcaccgac aaacagtgcc agcagcaagc gcggcagtcg gtactccatc aatacataat
     2101 aatgctcgcg tccggcctgc cagtcggtca gcagcgcgcg ccacggcacg gggatcactc
     2161 ccatatggag tgataacagc gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg
     2221 caattttcat cctcgcctcc tcacaagcca gacaaagcaa gggctgccaa tcagcgccag
     2281 cactgcgcct gcgggcagat ctccggggaa ggccagcgcg cgtgcgagta catctgccag
     2341 cagcatcagc gtggccccca gcagcatgct cactggcagt acgttgcgct gatcgaagcc
     2401 tgcccagaag cgcgccagat gtggcaccag cagaccgata aacgccaccg gacctgccac
     2461 gctgacgcac gcaccaacca gaagcagcac taacatattg atgaccaaac gtagcctcgt
     2521 caggttcact cccagcgtat gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt
     2581 cgccagcagc aacacgacag ggactgcagt gaccaccacc ggcaagagct gccagacatc
     2641 ctgccagcgg gcgtgggaca ctccgccagc cagccagtaa aagatgccgt aagcatgatc
     2701 ttcggccagc agcagggtga tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc
     2761 cgcgaggatc agtttgtttc tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac
     2821 cagcagccag ctcacgccgc ccccgcatgc cgcgatgaac gacagagaat agcctgcaat
     2881 cggcgtcgga ctcagcgcgc tggtaagcgc catagccagc gccgcgccgc tgttaatgcc
     2941 gagcagtgaa ggagaggcca ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag
     3001 cgccaggctt gcgccgatca gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac
     3061 cagcgcttct ggtagcgttg gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc
     3121 ggaaacagga atggccgagt agcaaaacag actcagccag aaaataataa taagtgctgc
     3181 aacgggaagc ccccacagca gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc
     3241 ggctgatggt ggaagatttt taccgtgtca gcggcaatac gctctgcagc aaaaataccg
     3301 cgcatccgcg cccaggtgtt actgtcgacc gaagcaacct gctgcttctg cgcggcggtt
     3361 aacatctgcc agagcggatc ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg
     3421 gcaaccagca gccaggcagg attgaccgcc agcagttgct ccaggccgat ggacggcatg
     3481 gacgcacccg ccatcgcagc gggaacgttc agccccagag aggccagcac gctgccggtc
     3541 caggtctcct gagtatgcag gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt
     3601 gtccctttgg gaagctggct ggcccactgc gccatcctct ctttatgttg ttccagacgt
     3661 gcctgcatct ctcgcttttt acccaccatt tcgccgatga tagccgcaga ttgcaaattt
     3721 tcagcgtagg tttcgttgcg ggacttaagc agcagtaccg acgcgatttg ctgcaaggcg
     3781 atgtaaaccc ccgcatggcg actgctgtcg gcaatgatca ggtctggttt cagagcggca
     3841 atggcttcca ggctcggctg cgcgcgcgtt ccgacggact gccacggttt caggtgcgca
     3901 cgcacttcgg gcaggatgcg ttttgcatcg ttatcgtcgg caataccgat cgggctgacg
     3961 tccacggcgg ccagcgcatc ggcgaacgag agttccagca ccacaatccg ttgtggcgtt
     4021 ttttcgagtg taaacgtgcc gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc
     4081 accagcagca ggcctgcaaa aagaaaacgg ataaatgcca acataatcac attccagcta
     4141 aaagcccggc aagccgggcg ttaacacatc agaacttcaa cgccccctgc atatacagcg
     4201 tgcgcggctg gcctgcatag atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt
     4261 cctggtcgaa gatgtttttc acaccgaatg ccaggttcag atctgccatc tgcgggccaa
     4321 agtcatacac cacgcgtgcg ccccagagca tgaagccggg aatgcggccg gtactgccgt
     4381 cggcgctctc tttcaccgta ttggcgttat ccgcaaactg gctggactgg aaatcgctgt
     4441 tcagattgaa catccagttt cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg
     4501 gggagaatgg taccagattg ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat
     4561 acgcatagct ggcgtagatg gaaacgttat caagcgttgg cgttagcgta cccagatcgt
     4621 aacgtgcctg cgtttccagc ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt
     4681 tggtctggtt ggagtcgtac tgattgttaa agttaatcag gaacagcccc atttccgccg
     4741 tcagcgcgcc gtcgtcgtag cgggtaccga gttcccaggt tcgcgctttt tccggttcca
     4801 cattgccgct ttgcacagcc ttgccaatct ggctgtactg tacggtgccg aacgagcctt
     4861 cagtgtttgc ataaagattc cagctgtcag tcaggtgata gagcacgttc aacgccggaa
     4921 gcggtgcgtt atagctcact tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact
     4981 cgatatgttc gaaacgcata cccggcgtga tggtccagtt gccgatgtcg attttgtcat
     5041 ccagatacca ggcgtgcgcc tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc
     5101 cggacggcaa ctgcccgctg ctggtggcgg tgtagtaacg catttcatgc gttgattcat
     5161 tcacatagcg atagcccacg cccacttcgt gcgcggaagg gccgatcata aagatctggc
     5221 tgtagcgtgg ctcaataccg cgcacccagt agttacgcgg cgagagggtg atgcgtttgc
     5281 cttgctccag gtagccgctg cgcagggttt gggtgtagaa cccctgaatg ttgaatttat
     5341 gctggctgtc tggctggaac tggtagccca ggctcgccag cttgcgacga ccccagaagc
     5401 ggtcatacgg gcgggtggat tgccagcgat cggcgtcgta atccgcgcga gacaggccac
     5461 cgggcatgtc ggcttcaccg tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat
     5521 ccggcgcata tttgcttttc agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc
     5581 agtcactgcc gcgcgtgccg gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc
     5641 cgcccaccat caggttgtgc gtctctttcg ggttgttttg tgaagaggtt gggctgagct
     5701 gaccttccac gcccgcctcg ataccaaagt cctgcggaat ggcacgggta acaaagttca
     5761 ccacgccgcc cacgctctgc ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa
     5821 tggcattcat gttgccgagc gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg
     5881 cgaaggggac ggggatgccg tccatcagga cggtcgagcg gctggcgagg cgcgggttca
     5941 ggccccggat gccaaagttc atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg
     6001 cgctgacgcc agggatgcgg ttaagtacct cacgcatggt ggttgcgccg gttttggcga
     6061 aatcctcacg gcggatcacg tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg
     6121 catcacccag ccagtcgccg accacggtca gggcatcttc ttttggtgcg ggcgcgggct
     6181 ccagcgtcca gctgttattt cccagcggtt ttacctgcag tccgctgccg tccagcagtt
     6241 gttgcaggcc gctctcgacg tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg
     6301 tcaggctggc gtcaaccgag agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt
     6361 tatcgagcga tcccggtgcg atattaacct gtgcagcaaa agcggaaaac gagagaccgg
     6421 ccagcggcag caggctcagg cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc
     6481 gtaacggcgt cataccttcc ccatcatcat ttttgttgtg ttcagctatg agtcgaacga
     6541 gaagggtaaa aaggacaatc gaaataagaa ttattttcct tacagtggtg aaatgtttat
     6601 ccagtaccgc gtaatagact gaattttaac gggaagcgtt tgcgcgataa cgttcaggat
     6661 cgcatcggta tttttcagcg ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc
     6721 gcagcgcagc acgccgttgc ggtaacgggt tagcgtggct atcacctcac ccagcggttt
     6781 atcgctgaag ctcaggatgt ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac
     6841 tgcgccaaac tcagaggcgc tgaactgcag gctttcacca gcgttcacga tgcgtttttg
     6901 cgcgggggca ctggcgagaa gcacttccac ggcgtgctgc tgcacgtcaa gctgcgtgaa
     6961 attatcctgc tggcggacgg taaattctgt ccctaaagca gtgagctggc cctgacgggt
     7021 caggacacgg aaggggcgtt gcagggcatc tttcgcggta gtaatggcga tttcaccgta
     7081 ccagagccgg acggtgcgct gatgcgcatc aaaacgcata tccgccgcgc tttgggtatt
     7141 cagcgtgagc agggagccat cttccagttg ctgacggctg actgtgcctt tggcggtgcg
     7201 gtaatctgcc cgcagacctt cgccggtttc cgactgccag agctgccagc ctccgccagc
     7261 gccgagcaac agcagtaatc ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa
     7321 cgcccggctg gcaacgtcgc caggcacacc gccaagctgg ttgcgcaggt tttcaacctg
     7381 ctgccaggcc cactggttat cctgatcctg ttcataccac tgttgccagc gcgcttcctg
     7441 ttgtgggctg acgcgctcgc cgcttagcac ggcataccag tgggaagctg aacgcagcgc
     7501 ctgacggcgg gaatcggtta acaaaggatt cataacccat actccagacg gaacagcagg
     7561 cagtgctcga cggctttcgc cacgtatttt ttcacggagc tgatggaaac accgagtttg
     7621 tgcgcaatct cgctgtatgt cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt
     7681 ttgccgttta gcccgtccag catgctgtcg aggagttgta gggtctcgag ttggctttcg
     7741 cgttcctcag gtgaaggcgc tcccccctcc ggcataagcg ccagcatctc cagatacgct
     7801 ttttccagcg cgtttcggcg aaacaggtcc accatcacgc gtttggcgat agtgcagagg
     7861 aaggagcgag gatcgcggat cgtcgagagc gtttcgctga ccattacccg caaaaaagtg
     7921 tcctgggcaa tgtcatctgc atcaaaagca gactggagtt tgcgcgtcag ccagcttttc
     7981 aaccagccgt gatgtgtgcc ataaagcgac tcgaatgtta aggaagctgt ggtagtggcg
     8041 cggtcagaca tgcggagtgc atcaaaagtt aattatcacg tagccatatt aatatgagaa
     8101 tagttatcat tacaattgga aataaaattg tttccaatag acatttttaa catgttgttt
     8161 ttctaagtgt tataaggtag gtataaaatg ggatggagcc tctgcttctg gcatgtgtcg
     8221 gtcagaatga ctcatgatgt ggtctgctat tattgacatc ctcactgccc taaaggatag
     8281 ggatttcggt aatgctgtca acttactgat ttagtgtatg atggtgattt taaggtgctt
     8341 gcgtggcttc catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta
     8401 acggcaaaag cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc
     8461 aactacagtt cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg
     8521 ccatgaatgg cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg
     8581 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag
     8641 tgatgtgatt gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg
     8701 tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt
     8761 cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt
     8821 cgtggtatag atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca
     8881 cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca
     8941 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
     9001 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttacctcctc
     9061 ccgaactcaa aa
//
LOCUS       DAZPJR010000166         8729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPJR010000166 DAZPJR010000000
VERSION     DAZPJR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.170"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /locus_tag="ACHVB7_003596"
     CDS             complement(<1..135)
                     /locus_tag="ACHVB7_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HFR1915483.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     P"
     gene            complement(132..2762)
                     /gene="traC"
                     /locus_tag="ACHVB7_003597"
     CDS             complement(132..2762)
                     /gene="traC"
                     /locus_tag="ACHVB7_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR1915484.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(2888..3235)
                     /locus_tag="ACHVB7_003598"
     CDS             complement(2888..3235)
                     /locus_tag="ACHVB7_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915485.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(3263..3481)
                     /locus_tag="ACHVB7_003599"
     CDS             complement(3263..3481)
                     /locus_tag="ACHVB7_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915486.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(3580..4053)
                     /locus_tag="ACHVB7_003600"
     CDS             complement(3580..4053)
                     /locus_tag="ACHVB7_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915487.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(4046..4459)
                     /locus_tag="ACHVB7_003601"
     CDS             complement(4046..4459)
                     /locus_tag="ACHVB7_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915488.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(4452..4673)
                     /gene="traR"
                     /locus_tag="ACHVB7_003602"
     CDS             complement(4452..4673)
                     /gene="traR"
                     /locus_tag="ACHVB7_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR1915489.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(4808..5323)
                     /gene="traV"
                     /locus_tag="ACHVB7_003603"
     CDS             complement(4808..5323)
                     /gene="traV"
                     /locus_tag="ACHVB7_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR1915490.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(5320..5640)
                     /locus_tag="ACHVB7_003604"
     CDS             complement(5320..5640)
                     /locus_tag="ACHVB7_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR1915491.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(5627..6217)
                     /gene="traP"
                     /locus_tag="ACHVB7_003605"
     CDS             complement(5627..6217)
                     /gene="traP"
                     /locus_tag="ACHVB7_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR1915492.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(6207..7634)
                     /gene="traB"
                     /locus_tag="ACHVB7_003606"
     CDS             complement(6207..7634)
                     /gene="traB"
                     /locus_tag="ACHVB7_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR1915493.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(7634..8362)
                     /gene="traK"
                     /locus_tag="ACHVB7_003607"
     CDS             complement(7634..8362)
                     /gene="traK"
                     /locus_tag="ACHVB7_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR1915494.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(8349..>8729)
                     /gene="traE"
                     /locus_tag="ACHVB7_003608"
     CDS             complement(8349..>8729)
                     /gene="traE"
                     /locus_tag="ACHVB7_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR1915495.1"
                     /translation="NSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRPGAQN
                     QMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTDIKHY
                     ILILKRENGVTWLDNFGETDDEKK"
BASE COUNT         2029 a         2405 c         2085 g         2210 t
ORIGIN      
        1 cggtgcgttc agtctgacaa tgccgtaact gacggctgcg ttcagtaaca ccattgccag
       61 gcatcccggc accgtccacc accagtggct gcgtcggggg gctgtaacat cagtcgtttt
      121 ttgcgttgtg gtcatgcaac actcctgtat ttctcatgtt cttccagcca ggcttccagc
      181 gaagccatct ccggccctga cttcttccac gccagctgcc acaccgcctc atgaatactc
      241 aggccctccc tgcgtttctg ctgcacaaac tcaaaatccg ggccgtcaga gctgtacatg
      301 gcgcggctta acgggtccac aaacagacgg tgccaggagg aatggttttc cacctgcagc
      361 aggaaggaac tgaaccactg gtctttggcc gcaccaaact tgccaatcat gtcgcgctgc
      421 agtggctgga actgatccgg gaataactgg ttatatttcg cgaactcctt cgcactctgt
      481 ttgaggataa ttttgtagga ggagttaccc catgccgcac gggccgcact ggaggccttg
      541 tcagagtcaa agtcgacgat gttctgtgtg atggtgatat aggcaccggt atggcggcga
      601 caggtgcggt aacctttttg aataaactca ccgaccttgc gatttttgaa gtccagcaga
      661 cgccagcctt catcaatgac gttcagtttc ttgagagtac gcggcgtgcg gtacatcctg
      721 ttctcgatgt agataatcag ggagaacatc accgcaacca gcagtgacgg acggtcttcc
      781 agtccgccca gctccagcac caccattttc gcatcatccc gcagggacgg ctcatcggag
      841 ttaaaatact ggccgtaagt gccgttggca gtgtactggt caagcagcac aatcatttcg
      901 tccagacggc tgcggatggt cggtgacccg gcatactgct cgctgtcact ggcgtttttc
      961 aggaaatcca ccacgtcatc aatacgtgcc ctgttctctt tggccagcca ggaggccctg
     1021 accgcctgca gcagcagacc ttcatgcact tcatccaggt taccgttggg gctggccatc
     1081 actgacaact ggtcacggac acgctccgct gactggtcaa tatcggtgat gttcgcaaac
     1141 gggttaaaac gcagggtttc accgtccaga tatacgcccc ccatgttctc acacagggac
     1201 ttgtagccat cccccatgtc gaacaccacg gcaaagcccc cggagtccag cacgctgcgg
     1261 ataagtggct gtatcagtcc ggttttaccg gccccggagg tgccacagac cgccatgttg
     1321 tagttggtgt tattcatccc cttaaagaag atatcgataa acgccagctg gttacggtag
     1381 gtgggcgcca gcagacctgc cggtgtcagg gggttatccg ccactaacgg catcaggttg
     1441 gccacattaa agctctctgc gcgctgaacc acaccggcct ctttcagctg tttaaacagc
     1501 cctttcccgg ccataaaggg caggcaggtc aggaaattgc gcatgtggtt aaagcgcggt
     1561 gaaatcagct caaaaccgtt tttacggaag ctgttcagga tgtcctgctc cacttccagt
     1621 gccgtttcat tattgtcctt gcagaaggct gtgatgttga ggaagtagga cacgacagaa
     1681 gactggccgg agcccagccg ctggcgcagt tccccccact ccttcgcctc tttctccacg
     1741 gacggaaacc atttggcata tgaggtcttc gacttcttct ccaggtccat gtacttcagg
     1801 ttggcttcgc tgtgggtttt cacctggtct tccaccacca gagtcagcgt caggatgaac
     1861 gggcaggaaa tggacatttc cgggttcagc agattgctgt agttgtcggc catgttccac
     1921 aggaaggcga tttccgggtt acgggccaga tggaaattca ggatgcgggc cgtgctgttc
     1981 ctgccgttct cacgcaggcc cagcgtcagg taatcagccc ggacattcag gtcaaaactg
     2041 tcctccacac actgataatt cagatcagaa tacgggtcca gctgacgtct tttcgggtac
     2101 agagagtccg gattatggtt aatcatctcc ccgacaatct cgatgaaagc ctgtgcatcc
     2161 actgtctgtg tggtgatttt tgcgccgtga aaagaggcac ggatgatttt caccagattt
     2221 tccatttcca gaatgtcagc ccggcttttt ttcttcgagg gagaacagta cgagataaac
     2281 acccggtaat gacgcagggt caggggcaga ttcatcccct ccggcagcgg gaactgtgtc
     2341 gctgccgctt tcatataata ggcacgggta atggcgttaa accgttcagc ctgttcacct
     2401 gaccaggaga actcacgcag cccgtattca atcctgtcac caaccagctg actggacatc
     2461 agatggatac acaacggaat accgcgcggc agtttggtgc gcagcatgtg atcgagtgcc
     2521 tccacaatgg acttattcgc cccattgatg ggaatggctt ccagcataaa gcccatcgtg
     2581 gtgtcattca tgaacagacc ggattcctgg ttgtaatcac ggtacggcag cagacggctg
     2641 aactgcggga agctcatttc gcccagaact tcattggcct ttgcggattc gtcaggcagt
     2701 ttcagtgctg tgacgaggga gttaaccgcc tgagtgacgg cctcaagtgg gttattactc
     2761 atatgagtat tccttagtgc gtttaaaaaa ggaaaatatc agtctgtggc cgtggatata
     2821 tcacgcagtg ccattcctga aaccacagca caaataagag atggaggctt ttaacaggaa
     2881 accaggatta acgttccttt actgaatgag catcatttaa tgcctgaatt tgtttttcgc
     2941 gaatcttatt gcgctcattt tctcttaccc gtttcacctc tgccatttcg tttctttttc
     3001 tgcattctga tgcgatttta tttgccttac caagtgttaa aggttcagac tgataattaa
     3061 gtgtctcctg aaatgcttct gctgcacagg gagtttcctg taaaatctct gtcagttgtt
     3121 ctggtgttgc aggttgttgc gcaaggtaaa cgatgtaaaa aggaatacat acaatcatag
     3181 ccataacgcc aacaaatata aaagccagaa cagattttaa atatatcgac ttcatttcat
     3241 tttccttcga agcagtaagc actcattgcc caccaccatt tagtgtttcc atcacgctgt
     3301 tgaaaggttc atttgtttta acttcaatac actcccctgt atttccaaag cccctgccgc
     3361 catgtgcttc gattgtccga agaacctgca cgcatacgtc cgtatttggc atgggtgtaa
     3421 tattccagct tccgccaccg ttaatatttt ttatcagcac aagaaaaata aaggtatgca
     3481 ttctttatta tctccgcttg tatattaatc actgaatatc ccttcagtta acttatgagg
     3541 tcataaaaca ctgcagattt tcattcagat ttactctctt cagcaaactt tactctcttc
     3601 agcaaacttc cggatggtat cctgttcacg ctgacgggtg gcagactcct gcattgcatc
     3661 cactttctcc tgccagagac gccggatatc cttttcatcc cgaccagtca gttttttacc
     3721 ggacagcaga cggttaagca gttcctgtct cgcatccggt acgtcttcag tcagacgcat
     3781 aagctgaagc aggctctgac ggctgagaac catgagtgat tccggcagga gggtgaagaa
     3841 gaaaataaaa aagcataccg caccacagca ggcaaggacc gcaaacagga ttttcagata
     3901 tataccatca acgccgaacg cagcaataat acagggaatc catgcaatac acgcacaata
     3961 cgccaggatg ttatctattc ggttatgtct gcgatatatc tgttgatgtt catccagaat
     4021 agaatctatt ttttcagctt ctgatttatg catagtgttt cctctgcgtt tcctgccaga
     4081 gacgccggat atcctgttca tcccgaccag tcagtttttt accggacaac aaacggttaa
     4141 gcagttcctg tctcacatct ggtacgtctt cagtcagacg cataagccgg agtaattcct
     4201 gacgactgag taccatgcgt gattcctccg gtacgctgta ataattaaaa atacgactga
     4261 ataccccaca acagcaggca acggacaggc acagggattt cagatacagg ccagaaacac
     4321 agaatgtggc aacaccacaa agaatagcta taactaaaaa tcccttaccc agtgccatac
     4381 acaaagaacc ctgttttctg gatatccgct catgctcatc cagaataaaa tctatttttt
     4441 cattttctga tttatgcata gtgtttcctc tgcctttcct gatacgcctg acattcaacg
     4501 cacaacgtca caccgggaaa tattttccgc cgggcttccg gaataggatt tccgcatgct
     4561 tcacagagat aaacaggaat accatgaaca tttatttcct ggcgtatccg gtttattcct
     4621 gtcatggtca gttgttctgt cactgaatat gcttcatcgg cttcatcact cacggaatat
     4681 tcctcccgac atgttctgac acgctaccgc ccatcccttt tcacgcacag ggcactcgtt
     4741 ttacaggcgg attttgaata cagaaagccc gccggcattg ccggctgata tacaacttca
     4801 gggccagtta attaatacgt ggttttcccc acgcagacgg tttaataaca aaaaacacgc
     4861 tggatggctg atgataaaca tcctggttat caatataagg cgctatccat aatgctgccg
     4921 tctgctcacc cgttcttagc ggacgaggag gtgttaccgg cgtaactgaa ctgaccggaa
     4981 caaccgtttt cacttcccgg gcagcggtaa acagcgggcg tggtgccagc agtttctgct
     5041 ccggatgtgc tgtcaccgca ggtctgctgc cggacggagt ggtcgcggtg acggtctgca
     5101 ccggcattgt ccggaagttc ccttcagcca ggcgcggcag tgatgccgca accggctttg
     5161 cttctgagga ctgctccagt tttttggcct tctcattggc ctgctccatc gtcatacagg
     5221 tatcggatgt ggtggcgtta cactcaaatt ccgtatttgt tcctgcgcag ccggaaagta
     5281 ataaggttcc cagcagagga ataaataaag aaatctgttt cattgccctt ctcctttttt
     5341 attactcccc acaacagcag aaattaactc tgctgtcaga tttgttttcc ctgcacaggg
     5401 atgtccggag accactgtat ttcccggaga agaaaataag ctcatcgaca ttcccttctt
     5461 catcacaccg acagcagcca tattaaattt gtgcggcaat gattcagaac tgaacacctt
     5521 cggagcagga agaactattt ttgtggtgca gttactgaga acagcatgca taaaatcatc
     5581 tgatacgttt ttatccggca caacgcgggc tgtaataaca ttaatattac gcatgttcat
     5641 ttttatctcc gttgcttctg tttgttcggt catcagggaa ataaacatgg catttcctga
     5701 tttttcccgg cagggaagcg tcttccgtat aaacgtcctt cagatattca tctgcggtta
     5761 ccacccggat acatttcatt tttccgggca gcaccggata tttcccggtg atcgctgcca
     5821 gcgagacata catcagtgcc agtgttgccc ataatgcggt gaccagttgc atcagtgacc
     5881 ggtcagtctc cctgacataa tctgcagcat cagccgcgcg gtaatggcag atatcctgta
     5941 tttctgatct gcctgaagaa tcagaggaag aaaaccagca ttcactgacc gggaactgtc
     6001 cggcaggtgc tgtttgtctt acgaagttaa tctccttaac cagtaatttc cccggcgtgg
     6061 tgttgtcctt ccataaaaca aacagagcca tcagggccat tgtggccagt ggcagaatga
     6121 cggtgtactt cagacaccag aacagtcctc gcagaacccg ggcgacaaca taccgggcgg
     6181 catggcacgc ctgacgggac gacatattat ttgccatcgt tgcctcctcc ctggctgccg
     6241 gacataaact gctgcggatt caggttgccc agctgcttca gcatctggtc agtattaaag
     6301 ccgttaagat gactttcagc tgatggcgga acaggaaccg gactttccgg attatcttct
     6361 tccgctctgt tctgcgtgcg ctccagcgcc atctcttcca cggttttcag ctggaagccg
     6421 tcctggaaca ccacggtcac ttcgttgccc gcaccaatcg gtatcaccgg gtgatactgt
     6481 tcggcacgtt tgatgtagta gtcactgagc gtctgtgcgg ctttcgatgt gccgccaccg
     6541 atacccattt tcaggacatc accagccccg taagcggctg tggcacccag cccgacagcc
     6601 ggctgggagg cgcgctccat tccctgaccg ataccgtcaa caaatcccgc gccccatgcc
     6661 cagccgagga ttttgccgtt acgcatcacc acttcgccct tgataccgtt tttaccccgg
     6721 aagctgacat gccccttaat cggcatatca atagttttgc cgtccttcag gcaactgata
     6781 ttgcgggtgc gtacaatggc acgctcactg gacacatccc cccaggcttc cagccccaca
     6841 aaacatcccg ttgcgtcata cgtcttgctg ttcggcattt ccaccaggcc ggtgatacgc
     6901 aactgcatcg gcaccgtgga ttcattaccg gtgactgagg cattggcatc cgccccttca
     6961 atcagcatgg ctttcgcaaa acttcctgac ggaatgtacg gcagcgatgg tccctgtttt
     7021 ccctcattac gggtaaacac cttacgctgt atccggttag gcaccggcac agactggtac
     7081 gtcacctgtg gtggtggcgt gacaccattc ccgggataaa acgccgtcgg cggtggaaca
     7141 gcaacgctgc cctgcggcgg gaaggatacg ggggtgtttc ctggctgtgg ttcgccttcc
     7201 ggcccgggcg gtgaggcagg catctgaggt acaggctcac cggtcgccgt gacgggatta
     7261 gcacccaggg cttttacctg ctctgccagg gcggcattgt cctgtcccag cttttcaata
     7321 cgacgctggt catcaccgcg ctgtttgttc aggacatcca gttcacgacg gatttcctca
     7381 tactgcttct gcatctgcgc tgccgtcacc tgcatctctg tggtggcatg ctgacgcacc
     7441 ttgtcatcaa aggtcgtatc caccacacct gtcatatccg gcacaggctc ctgttcagcc
     7501 accgcctcac cgttaccgga catgtccaca tcagacagat acagtacccc accaatcgcg
     7561 gaggctgcac cgacaaccac aatccccagc cacaggtact gcttgcgttt cacaatggta
     7621 ttgatactgg ccatcagttg ccctccccgt cacggatgac atacacatcc atgcgcgcac
     7681 ctgccagtaa ctggctggca ggctggctga acatcacggc acgggttccc ggctgccaga
     7741 agtcactttc ccggatattc agggcagaca gcgttttatt ttccacggca aagcggacca
     7801 tcttcaggtg attccccgtc catacggcat ctgccactga agacagcccc gccggggcct
     7861 gcagggtttc ctttgtcaca ggaacctgat accagcctgc aggtaatttt ccgccacgga
     7921 cagcctggct gatggtcaca agcagggatt cgtaaggcgt ggacgtttcc cacgctccgg
     7981 cttcttctcc ggttccgcgc aggtcactga ccagctgaat ggtacgcccc gcgccttcac
     8041 ggggaacagc ctgaatggaa aggttcagac cacgctctgt ttccagaatg aacgtaaagg
     8101 gctttttgtt gacggtggcc accaccacac cgccggaggc ggtctgctca ttattggtca
     8161 gggcaccatc cagactgttc acggcgataa tccggtcacc gggaacggta aacagattcg
     8221 ggctggtatt gctgatggca atactggcct gaccaccgtt caccattggc accacagtgg
     8281 gggccagcgt accgtttgcg gccatcacgc tgcaggaaaa aaacaggccg ccgaatatta
     8341 ttgccgtatt attttttctc atcgtctgtt tccccgaaat tatccagcca ggtcaccccg
     8401 ttttcccgct tcagaataag gatgtaatgt ttgatatcag taaaaggttc ggagttacca
     8461 atccatgttt tccgaattcc acggatttcc acacgaccat actgaggcca gacacggaca
     8521 ctggtctgga aaaaagcaga attcacgtta tcttttttaa tgagtttcgc ttcttcagcc
     8581 agaataactt tcatctggtt ctgtgcaccc gggcggatat attgcagaag cgcctgatgt
     8641 gaagcatcga cgatttccgg tgagacattc agacggaggg caataaatga cagcgccatc
     8701 tgctgcaaat aagaggcatc ggcactgtt
//
LOCUS       DAZPJR010000167         8636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPJR010000167 DAZPJR010000000
VERSION     DAZPJR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.171"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHVB7_003609"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHVB7_003609"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR1915496.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHVB7_003610"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHVB7_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR1915497.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="ACHVB7_003611"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="ACHVB7_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR1915498.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="ACHVB7_003612"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="ACHVB7_003612"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR1915499.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="ACHVB7_003613"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="ACHVB7_003613"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR1915500.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /gene="ymdA"
                     /locus_tag="ACHVB7_003614"
     CDS             complement(7826..8137)
                     /gene="ymdA"
                     /locus_tag="ACHVB7_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR1915501.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="ACHVB7_003615"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="ACHVB7_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR1915502.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2157 a         2248 c         2051 g         2180 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aaagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatcccct tactgacatt
     4921 gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
     5281 tatcctcggt gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttac tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg ctccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc ggacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaacgga ctggattcca tttggcgtaa gtaacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacgggtcct attgccataa catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gtcctcccac cagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
     6841 atataaccaa gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cggggatggc tgtcaagcaa gcgtaaaata tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgcagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gcgttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc
     7921 tcattaacgc tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac acccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 gccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aataggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata atggtataca
     8521 catccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaac agggcttgcg ccctgtttct gtaata
//
LOCUS       DAZPJR010000168         8594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPJR010000168 DAZPJR010000000
VERSION     DAZPJR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.172"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(293..499)
                     /locus_tag="ACHVB7_003616"
     CDS             complement(293..499)
                     /locus_tag="ACHVB7_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR1915503.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            812..901
                     /gene="kdpF"
                     /locus_tag="ACHVB7_003617"
     CDS             812..901
                     /gene="kdpF"
                     /locus_tag="ACHVB7_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR1915504.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            901..2574
                     /gene="kdpA"
                     /locus_tag="ACHVB7_003618"
     CDS             901..2574
                     /gene="kdpA"
                     /locus_tag="ACHVB7_003618"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR1915505.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2597..4645
                     /gene="kdpB"
                     /locus_tag="ACHVB7_003619"
     CDS             2597..4645
                     /gene="kdpB"
                     /locus_tag="ACHVB7_003619"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR1915506.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4654..5226
                     /gene="kdpC"
                     /locus_tag="ACHVB7_003620"
     CDS             4654..5226
                     /gene="kdpC"
                     /locus_tag="ACHVB7_003620"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR1915507.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5219..7903
                     /gene="kdpD"
                     /locus_tag="ACHVB7_003621"
     CDS             5219..7903
                     /gene="kdpD"
                     /locus_tag="ACHVB7_003621"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR1915508.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7900..8577
                     /gene="kdpE"
                     /locus_tag="ACHVB7_003622"
     CDS             7900..8577
                     /gene="kdpE"
                     /locus_tag="ACHVB7_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR1915509.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1797 a         2239 c         2480 g         2078 t
ORIGIN      
        1 accgccatac tgcgtcatgt cgccctgacg cgccgccggt tttccgctca tttctcatcc
       61 ttgttttatc gggttatttg tccgctgaca ggcaagaccc tacagattta taagaaagcc
      121 ttaagtgaga tatccgtcag atagaaacaa ttaggaaaat atttatatat atttcatgaa
      181 tatatgtttc gattggtata acggtgtcta aatattcctc tgaacatctt cctgatacaa
      241 aaaaccgcca gcgtttccgc tggcggtttt caatgtattc agaactcagc aatcagtaaa
      301 aatcaccggt tgccttttcg gcctgatcca tccacaccgg tttatcgctg gttttcgacc
      361 agacgcggtg caggtagctg taaaagcggg cgcggtcttt ccagaaaagc atgaaaggca
      421 gcgccagaac gcccgctgca accgcatatg tacggcgtaa aaagataagc catgcaggat
      481 attctctgta gagttccata cttttctccc ccttttgtat ctacccggtg aatagcaccg
      541 gaaaaatgaa tttgtttatc tgatgaaaat agtaccgcct tttgtgtaat tttactactc
      601 atccgaccac ttatttttgc ttattgatgg tttatttaca ttcactctgt aattaagtta
      661 cacaaaagtt aaattaatac taaacattag ttaaatcatg gcttttgcca tttttatact
      721 ttttttacac cccgcccgca gatttttgcg aaatctttgc agccagaatt ctactcttcc
      781 ggtatcactt ttaggccact ggaggtgcac tgtgagtgca ggcgtgatag ccggcgtgtt
      841 gctggtgttt ttattactgg gttatttggt ttatgccctg atcaatgcgg aggcgttctg
      901 atggctgcgc aagggttctt actgattgcc acgtttttac tggtattaat ggtgctggcg
      961 cgtcctttag gcagcgggct ggcgcggctg attaatgaca ttcctcttcc aggtacagcg
     1021 ggcgttgaac gtatactctt tcgtttacct ggcgtctctg accatgagat gaactggaag
     1081 caatatcttt gtgccattct cggcctgaac atgctggggc tggcggtgct gttttttatg
     1141 ttgctcggtc agcactatct gccgcttaat ccacagcagt tgccggggct gtcgtgggat
     1201 ctggcgctga ataccgccgt cagctttgtc acgaatacca actggcaatc ttatagcggt
     1261 gaaaccacgt tgagctattt cagccagatg gcaggcttaa cggtacaaaa ctttctttct
     1321 gccgccagcg ggattgcggt gatttttgcc ttcatccgtg cgtttactcg ccagagcatg
     1381 agcacactcg ggaatgcctg ggtcgatctg ctacgcatca cgttatgggt gctagtccct
     1441 gtggcgttgt tgatcgcact gttttttatt caacaaggtg cgctgcaaaa ctttctgccc
     1501 tatcaggcag tgaataccgt tgaaggagcg caacagctgt tacccatggg accagtagct
     1561 tctcaggaag cgatcaagat gctcggtact aacggcggtg gcttctttaa tgccaactcg
     1621 tcgcatccgt ttgaaaaccc aaccgcactg accaacttcg tgcagatgct ggcgatcttc
     1681 ttgatcccaa cagcgctgtg ctttgccttt ggtgaagtga cgggcgatcg ccgccagggg
     1741 cgcatgttgc tgtgggcgat gtcagtgatt tttgtcatct gcgtaggcgt ggttatgtgg
     1801 gcagaagttc agggtaatcc tcatctgctg gcgctcggcg cagacagcag catcaatatg
     1861 gaaggtaaag agagccgttt cggcgtgctg gtcagtagcc tgtttgcggt cgtgactacg
     1921 gcggcgtcct gtggcgcggt gattgcgatg catgattcgt ttaccgctct cggcggcatg
     1981 gtgccgatgt ggctaatgca aattggtgaa gtggtgttcg gcggtgtcgg ttctggtctt
     2041 tacggcatga tgctgtttgt cctgctggcg gtgtttattg ccgggctgat gattggtcgt
     2101 acaccggaat atctgggtaa aaaaatcgac gtacgcgaga tgaaactgac tgcactggcg
     2161 attctggtta ctccaacact ggtactgatg ggcgcggcgt tggcgatgat gaccgacgcc
     2221 ggacgtagcg ccatgcttaa ccctggcccg catggtttta gcgaagtgct gtacgccgtg
     2281 tcgtccgccg ctaacaacaa cggcagcgcc tttgccggat taagcgccaa ctctccgttc
     2341 tggaactgtt tactggcgtt ctgcatgttt gtcggtcgct tcggggtgat tatcccggtg
     2401 atggcaattg ccggttcgct ggtgagtaaa aagagccaac ccgccagctc cggcacgctg
     2461 ccaacgcacg gcccgctgtt tgttggcctg ttaatcggca ccgtgttgct ggttggcgca
     2521 ctgaccttta tccctgccct ggcgcttggt ccggtggcgg aatatctctc ctgatgatat
     2581 tgagcgagca ctgaatatga gtcgtaaaca actggcgcta ttcgaaccaa cacttgtcgt
     2641 tcaggcgctg aaagaagcgg tgaaaaaatt aaacccgcag gcgcaatggc gcaatccggt
     2701 gatgtttatc gtctggatcg gcagtctgct gaccacctgt attagcatcg cgatggcaag
     2761 cggtgcgatg cctggcaatg cgctgtttag cgcggcaatt agcggctggc tgtgggtcac
     2821 cgtactgttc gctaatttcg ccgaggcgct ggcagaaggc cgcagtaaag cgcaggccaa
     2881 cagtcttaaa ggggtgaaaa aaactgcctt tgcccgcaag ctgcgtgagc cgaaatatgg
     2941 cgctgcggcg gacaaagttc ctgccgatca actgcgtaaa ggtgacatcg tgctggtaga
     3001 agctggcgat attatcccct gcgatggtga agtcattgaa ggcggcgcat cggtcgatga
     3061 aagcgccatc accggggaat cggcaccggt gatccgtgaa tccggcggcg attttgcctc
     3121 cgtcaccggc ggcacgcgta ttctttctga ctggctggtg attgagtgta gcgttaaccc
     3181 cggtgagaca tttctggatc ggatgatcgc gatggtggaa ggcgcacagc gacgcaaaac
     3241 accgaacgag attgccctga ctatcctgct gattgctctg accatcgtct ttttactggc
     3301 aaccgccacg ctgtggccgt tttccgcgtg gggcggtaat gccgtgagtg taacggtact
     3361 ggtggcgctg ctggtctgtc tgatcccaac cactattggc ggcctgttgt cagcgatcgg
     3421 cgtcgccggg atgagtcgga tgctgggcgc gaatgtgatt gccaccagcg gacgtgcagt
     3481 tgaagcggca ggtgacgttg acgttctgct actggataaa accggcacca tcacactcgg
     3541 taaccgtcag gcgtcggagt ttatccccgc gcagggcgtg gatgaaaaaa cgctggctga
     3601 cgccgcacaa ctggcttcgc tggctgatga aacgccggaa ggtcgcagta ttgtgatcct
     3661 cgccaagcaa cgttttaacc tgcgcgagcg cgatgtgcag tcgctccatg ccacctttgt
     3721 accgtttact gcgcaaagcc ggatgagcgg gatcaacatc gacaaccgca tgatccgtaa
     3781 aggttctgtc gatgccattc gccgccatat tgaggctaac ggcggtcact tccctgccga
     3841 tgttgatcaa aaagtcgatc aggttgcgcg tcagggagcc acgccgctgg tggtggtgga
     3901 aggttctcgt gtgctgggcg ttattgcgct gaaagatatc gtcaaaggcg gtattaaaga
     3961 gcgcttcgcc cagctgcgca aaatgggcat taaaacggtg atgattaccg gcgataaccg
     4021 tctgactgcc gccgcgatag ctgcggaagc gggtgtcgat gattttctcg ccgaagcaac
     4081 accggaggcc aagctggcat tgatccgtca gtatcaggcg gaaggtcgtt tggtagcgat
     4141 gaccggcgac ggcaccaacg atgctccggc gctggcgcag gcagatgtcg cggtggcgat
     4201 gaactccggc acccaggcgg cgaaagaggc gggcaatatg gtggatctcg actctaaccc
     4261 gaccaagttg atcgaggtgg tgcacattgg caaacagatg ctgatgaccc gtggctcgct
     4321 gaccaccttc agcattgcca acgatgtggc gaaatacttc gccattattc cggcggcgtt
     4381 cgcggcaaca tatccgcagt taaatgcgct gaacatcatg cgcctgcatt cgcccgactc
     4441 cgccattctc agtgcggtca ttttcaacgc cttgattatc gtctttttga ttcctctggc
     4501 gttaaaaggc gtgagttata aaccgcttac cgcttctgcc atgttgcgcc gtaacttatg
     4561 gatttacggt ctgggtgggc tgctggtgcc gtttattggt atcaaagtca ttgatttact
     4621 gctgaccgtt tgcggtctgg tgtgaggttt accatgagtg gattacgtcc ggcattatcg
     4681 acatttctct ttctgttatt gattactggc ggcgtttacc cgctgctgac caccgcactg
     4741 gggcaatggt ggtttccctg gcaggccaac ggttcgttga tccgtgaagg tgatacggtg
     4801 cgtggctcgg cattaatcgg gcagaatttt accggcaacg gctattttca tggtcgcccg
     4861 tcagcaacgg ctgaaatgcc ctataatcca caggcttctg gcgggagcaa tctggcggtc
     4921 agtaaccctg agctggataa actaatagcc gcacgcgttg ctgcattacg ggccgctaac
     4981 ccggatgcca gcgcgagcat tccggttgaa ctggtgacgg catcggcaag cgggctggac
     5041 aataatatca ccccgcaagc ggcggcctgg cagatcccac gcatcgcgaa agcgcgtaat
     5101 ctcagcgttg aacagctcac gcagctgatc gcaaaataca gccaacaacc gctggtgaaa
     5161 tatatcggcc agccggtcgt caacattgtc gaactcaatc tggcgctgga taagcttgat
     5221 gaataacgaa cccttacgtc ccgaccccga tcgtctgctg gaacaaactg ccgcgccgca
     5281 tcgggggaag ctgaaagttt tcttcggtgc ctgtgcaggc gtcgggaaga cctgggcgat
     5341 gctggcagaa gcccagcgac tgcgggcgca agggctggat attgtggttg gcgtggtaga
     5401 aacccacggg cgaaaagata ccgccgcaat gctggaaggg ctggctgttc tgccgccaaa
     5461 acgcctggcg taccgtgggc ggcacatctg cgagtttgac ctcgatgccg ccctcgcccg
     5521 ccgtccggcg ctgatcttaa tggacgaact ggcgcacagt aatgcgccag gttctcgtca
     5581 ccctaaacgc tggcaggaca tcgaagagct gctggaagca ggcattgatg ttttcactac
     5641 cgtcaacgtt cagcatctgg aaagtctgaa tgatgtggtc agcggcgtca ccggaattca
     5701 ggtacgggaa accgtgcccg atcctttttt cgatgccgcc gacgacgtgg ttctggtgga
     5761 cttgcccccg gacgatctgc gccagcggct gaaagaaggc aaagtctata ttgccgggca
     5821 ggcggagcgc gccattgaac attttttccg caaaggcaat ctgatcgccc tgcgcgaact
     5881 ggcactgcgc cgtactgccg atcgcgttga tgagcaaatg cgcgcctggc gggggcatcc
     5941 tagcgaagag aaagtctggc acacgcgcga cgcgatcctt ttatgcatcg gccataacac
     6001 cggtagtgag aagctggtcc gcgcggcggc gcggctggcg tcacggctgg gtagcgtctg
     6061 gcacgcggtg tatgttgaaa cccctgccct gcatcgctta ccggagaaaa aacgtcgtgc
     6121 aattctcagc gccttacgtc tggcgcagga actgggcgcg gagacggcaa cactttctga
     6181 tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa cataatctcg gcaagattat
     6241 tctcggtcgc ccggcctcgc gccgctggtg gcgtcgggaa acgtttgctg accgactggc
     6301 gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac ccgcccgcac
     6361 gattaacaac gcgccggata gccgctcttt taaagacaag tggcgggtac agattcaggg
     6421 atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta attgccatgc agtggctgat
     6481 ggcgtttgat gccgccaacc tggtgatgct gtatctgctt ggcgtggtgg tggtggcgct
     6541 attttatgga cgctggcctt cggtggttgc caccgtcatt aatgtggtga gtttcgatct
     6601 cttttttatc gccccacgcg gcacgctcgc cgtctctgat gtgcaatatc tgctgacctt
     6661 cgcggtgatg ttaaccgtcg ggctggtgat cgggaacctt actgctggcg tgcgttatca
     6721 ggcgcgggta gcccgttacc gcgagcaacg cacacggcac ttatatgaaa tgtcgaaagc
     6781 tctggcggtg ggccgcagtc cgcaggacat cgccgccacc agcgaacaat ttattgcctc
     6841 cacgtttcat gcccgcagtc aggtgttgtt gcccgatgac aacggtaaat tgcagccgtt
     6901 aacacatccg caaggaatga cgccatggga cgatgccatc gcgcagtgga gttttgataa
     6961 aggcctgcct gcgggcgcgg gcaccgacac gttacccggt gtaccgtacc agattttgcc
     7021 gctaaaaagc ggcgagaaaa cctacgggct ggtggtggtg gagccgggga atctgcgcca
     7081 gttgatgatc ccggaacagc agcgcctgct ggagacgttt acgctgttag tcgccaatgc
     7141 ccttgagcgg ctgacgctaa ccgccagcga agaacaggcg cggatggcaa gcgaacgtga
     7201 acagatccgc aacgccctgc tggcagcgct ttcgcatgat ttacgcacgc cgcttacggt
     7261 gctgtttggt caggcagaaa tcttaacgct cgatctggca agcgaaggat caccccacgc
     7321 ccgccaggcc agcgagattc gtcagcatgt gctgaacact acccgactgg tgaataatct
     7381 actggatatg gcgcgaattc agtccggcgg ctttaatttg aagaaagagt ggttaacgct
     7441 ggaagaagta gtcggcagcg cgctgcaaat gctggaaccg ggtttatcgt cgcccatcaa
     7501 tctttctctg ccagaaccgc tgaccttaat ccacgttgac gggccactct ttgaacgggt
     7561 gctgattaat ctgctggaga acgcggtgaa atatgcgggt gcgcaggccg aaattggtat
     7621 cgatgcccac tttgagggcg aaaatctaca actggatgtc tgggataacg gccccggtct
     7681 tccgccaggc caggagcaga cgatatttga taagtttgct cgcgggaata aagagtcggc
     7741 ggtgccgggt gtcgggcttg ggttggcaat ttgtcgggcg attgtggatg tacacggcgg
     7801 caccattacc gcgttcaacc gaccggaagg tggtgcctgt tttcgtgtta cacttcccca
     7861 gcaaaccgct cctgaacttg aagactttca tgaggatatg tgacaaacgt tctgattgtt
     7921 gaagatgaac aggctattcg tcgctttctg cgcacggcgc tggagggcga cgggatgcgc
     7981 gtctttgagg ccgaaactct gcaacgcggc ttgctggaag cggcaacccg taagccagat
     8041 ttgattattc tcgatctcgg cctgcccgat ggtgatggga ttgagtttat ccgcgacctg
     8101 cgccagtgga gcccggtgcc ggtgattgtg ctttccgcac gcagcgaaga gagcgacaag
     8161 atcgccgcgc tggatgccgg agcggatgat tatctgagta agccgtttgg cattggcgaa
     8221 ttgcaggccc gtctgcgcgt cgctttacgc cgccactccg ccaccgccgc gcccgatccg
     8281 ctggtgaaat tttccgatgt taccgtcgat ttagccgccc gcgtgattca ccggggtgag
     8341 gaagaggtgc atctcacacc aattgagttc cgcctgctgg cggtgctgct caacaatgcc
     8401 ggaaaagtac tcacccagcg ccagctcctt aaccaggtgt gggggccaaa cgcggtcgaa
     8461 cacagtcact atttgcgtat ttatatggga catctgcgac aaaaactgga acaggatccg
     8521 gcccggcctc gccacttcat tactgaaacc ggtattggat atcggtttat gctttgaatt
     8581 gttattcaat aaat
//
LOCUS       DAZPJR010000169         8544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPJR010000169 DAZPJR010000000
VERSION     DAZPJR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.173"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            157..1479
                     /gene="dcuC"
                     /locus_tag="ACHVB7_003623"
     CDS             157..1479
                     /gene="dcuC"
                     /locus_tag="ACHVB7_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1915510.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(1522..2019)
                     /gene="sspB"
                     /locus_tag="ACHVB7_003624"
     CDS             complement(1522..2019)
                     /gene="sspB"
                     /locus_tag="ACHVB7_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR1915511.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2025..2663)
                     /gene="sspA"
                     /locus_tag="ACHVB7_003625"
     CDS             complement(2025..2663)
                     /gene="sspA"
                     /locus_tag="ACHVB7_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR1915512.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMCLGRS"
     gene            complement(3058..3450)
                     /gene="rpsI"
                     /locus_tag="ACHVB7_003626"
     CDS             complement(3058..3450)
                     /gene="rpsI"
                     /locus_tag="ACHVB7_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR1915513.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3466..3894)
                     /gene="rplM"
                     /locus_tag="ACHVB7_003627"
     CDS             complement(3466..3894)
                     /gene="rplM"
                     /locus_tag="ACHVB7_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR1915514.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4113..5240)
                     /gene="zapE"
                     /locus_tag="ACHVB7_003628"
     CDS             complement(4113..5240)
                     /gene="zapE"
                     /locus_tag="ACHVB7_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR1915515.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5434..5832
                     /gene="zapG"
                     /locus_tag="ACHVB7_003629"
     CDS             5434..5832
                     /gene="zapG"
                     /locus_tag="ACHVB7_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR1915516.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5986..7353
                     /gene="degQ"
                     /locus_tag="ACHVB7_003630"
     CDS             5986..7353
                     /gene="degQ"
                     /locus_tag="ACHVB7_003630"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR1915517.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            7443..8510
                     /gene="degS"
                     /locus_tag="ACHVB7_003631"
     CDS             7443..8510
                     /gene="degS"
                     /locus_tag="ACHVB7_003631"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR1915518.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT         2072 a         2163 c         2187 g         2122 t
ORIGIN      
        1 taatcataag cgcatatttt ttaatgaaaa atcacatcac ctacaattaa aaacacgaca
       61 tccgcaccat aaatagactt gcaaaaaata taacatcatt gttttcaatc tgccgtttat
      121 gggattgacc gttttctttt gacacggagt tcaacaatgt tcggcataat tatatctgtc
      181 atcgtattaa ttacgatggg ctatttgatc ctgaaaaact acaaacctca ggtggtgctg
      241 gctgccgcag gtgtcttcct gatgatgtgc ggagtctggt tagggttcgg tggtgtactc
      301 gatcccgcca aaagcagcgg ctacttgatc gtcgacattt ataatgaaat cctgcgcatg
      361 ctgtccaacc gcattgccgg attggggctg tcgattatgg cggtgggcgg ttatgcccgc
      421 tacatggagc gcataggagc cagtcgcgcg atggtgagct tgctaagccg cccgttaaaa
      481 ctcattcgct cgccgtatat tattctgtcg gcaacttacg tcatcggcca aatcatggcg
      541 cagtttatta ccagcgcctc cggtctgggt atgttgctga tggtcacctt atttccgacg
      601 ctggtgagtc tgggagtaag tcgcctctct gcggtggcgg ttatcgcaac cacgatgtcc
      661 attgagtggg ggattctgga aacgaactcc atttttgcag cccaggtcgc gggaatgaaa
      721 attgccacat acttcttcca ctaccagctt ccggtcgcct cttgcgtcat tatctcggtg
      781 gcgatctccc actttttcgt gcaacgcgct tttgacaaaa aagataaaaa tatcaatcac
      841 gaacaggcag agcaaaaaac tctcgataat gtcccgccgc tctattacgc cattttacct
      901 gtgatgccgt taatcctgat gctcggctcg ctgttcctcg cccacgtcgg gctgatgaag
      961 tcagaactgc atctggtggt ggtgatgtta ctaagtttga cggtgacgat gtttgttgag
     1021 ttcttccgca agcataactt gcgcgaaaca atggacgatg tgcaggcgtt ttttgacggc
     1081 atgggtacgc agtttgccaa cgtggtaacg ctggtggtcg cgggtgaaat atttgcgaaa
     1141 ggcttaacga cgattggcac tgttgatgcg gttatcaggg gtgcggagca ttctggtctg
     1201 ggcggtattg gcgtgatgat tattatggcg ctagtcattg ccatttgtgc cattgtgatg
     1261 ggctctggca atgcgccgtt tatgtcattt gccagtctta ttccgaatat cgcagccgga
     1321 ctacatgtac cagcggttgt aatgattatg ccgatgcatt ttgccacgac gctagcgcgc
     1381 gcggtttcgc cgattactgc ggtggtgaag cggacagcga tccccatggc agtcggtttc
     1441 gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa gacaaaacag
     1501 gccgcctggg cctgttttgt attacttcac aacacgtaat gccggtcgac caccgcgtgg
     1561 tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct tgtcgccatc
     1621 aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca tgatgctggt
     1681 atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt cacgggcgta
     1741 gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac caaagcgcgc
     1801 gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg gcgcaatgtt
     1861 gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag ggagcgtcac
     1921 atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga atgcacgcag
     1981 cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact ccggcccaga
     2041 cacatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc aaagacgcgg
     2101 gtcatatagc ctttcagctc tttcgcaccc gggccgctga gctcgatacc cagttgcggc
     2161 agacgccaca gcagcggagc aagatagcaa tcgaccaggc tgaactcatc gctcaggaag
     2221 tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag ttgcttacgt
     2281 gcggcatctg cttcagaagc tgaaccgttg atgatggtgt tcatcagcgt gtaccagtct
     2341 ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta aacaggcatc
     2401 agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga ttcccacagg
     2461 gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc aatcagatcc
     2521 tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt ctcagccagc
     2581 acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt cattaccgaa
     2641 cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt ttactgctac
     2701 cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact ctctttgttc
     2761 gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg atgatagttt
     2821 accagatttt gcgaccattg tggtgagtgg atgccggaaa tggggaaaaa gagatgcgct
     2881 ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt ttgcctgtct
     2941 ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat ttagccataa
     3001 aaaaacccgc cgaagcgggt tttttcgtaa attgttttct gccggagcag aagccaatta
     3061 acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct tacgttcaac
     3121 ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca gggactcgtc
     3181 gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac cagagatacc
     3241 accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca gttccagcgg
     3301 ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag aacgttggtt
     3361 gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaat ttttgcggcg
     3421 accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga tgtcaagaac
     3481 ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca gtttacggaa
     3541 catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt caatcacacg
     3601 ctcaggacgg cgagcaatca tctcttcaaa gctcacttgt ttgataccac cgatgtggcc
     3661 ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa ctttgtcagc
     3721 gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtgtattccg ctttgtgctt
     3781 accgcgtagg cgacgagcca gttcagtagc cagacggccc agagttttac cggtcgcgtc
     3841 aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt tcattaaaag
     3901 cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt gttgaggttc
     3961 acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac acaaaaagtt
     4021 ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt cggggtcaaa
     4081 gatcgacccc tttttgtgat ttgtgacagg ttttaacccg ccaaatgctc gcgcttcaga
     4141 tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc aaacttcagc
     4201 cgatcgccct gataaatttc atacagcggc acttctgcac tcaccactaa tttgacatgg
     4261 cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc catcaaccgc
     4321 gtcataactg gtacatcaaa caacatgacc gtatgaaaga gacgtgagag cgcaatatag
     4381 tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag agaccgccag cgtctggttc
     4441 tcgacgccca ttgtcgctaa tggccgatgg ttgatttcta acgtcggtga attttctcgt
     4501 ttcccccccg ccagcgccaa ccatagttta tccatttgcg cccgggtttc atcgtggagt
     4561 ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac accagcgtcc
     4621 acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa acgcgcacgt
     4681 tgcaagccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac cagggtaata
     4741 ccgcgggcga acaaggcttt catcagaccg ccaagtagca tggcatcggt aatatcagaa
     4801 acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc ggcaataatt
     4861 tccagcggat cggtctgccc ctgtaaggca gttagctctt catgcacccg cagcataaaa
     4921 cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa aaggtccatc
     4981 agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac tggcgtatgc
     5041 tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc actcgtcctg
     5101 ggggctggtg gcgtgctatt gatgagttcc tgataaataa tttccaggcg gctgacggcc
     5161 tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag gtattgcgat
     5221 gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt gcgccgttgt
     5281 tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg gattccactt
     5341 ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct gaagccaaca
     5401 ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc gctaattggg
     5461 ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg taaactacgc
     5521 cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga cgagtatcgc
     5581 gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat ggcgcacgac
     5641 tatcgccagc tgtatcagca catggcgaaa agttccagca gcctgctgcc ggaactgtct
     5701 gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa cgatcaggca
     5761 ccggtgcaga tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg tactggcgcg
     5821 aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct ctccctcccc
     5881 gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata acatcaaact
     5941 gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa aaaacaaccc
     6001 cagctgttga gtgcattagc gttaagtgtc gggttaactc tctcggcgtc atttcaggcc
     6061 gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct ggctccaatg
     6121 ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc cagtcaggga
     6181 cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga gcaacctgca
     6241 caacccttcg aaggtttagg ctccggtgtc atcatcaacg ccagtaaagg ctatgtgctg
     6301 accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa tgatgggcgc
     6361 gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct gttacaaatt
     6421 caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt gcgcgtcggt
     6481 gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac ctctggcatt
     6541 gtttccgcat taggacgcag cgggttgaat cttgaaggtc tggaaaactt tatccagaca
     6601 gatgcttcca ttaaccgcgg taactccggc ggtgcactgt taaaccttaa cggtgagtta
     6661 attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat tggatttgcc
     6721 atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg tgaaatcaaa
     6781 cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa agccttcaac
     6841 cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caggttctgg ctcggcgaaa
     6901 gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct gaatagcttt
     6961 gctgagttgc gctctcgtat cgcgaccacc gagccgggca caaaagtgaa acttggcctg
     7021 ctgcgtaacg gcaaaccact ggaagtagaa gtgacgctcg ataccagcac ctcttcgtcg
     7081 gccagcgctg aaatgatcgc gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta
     7141 aaagatggcg gcaaaggtat taagatcgat gaagttgtca aaggaagccc agctgctcag
     7201 gctggcttgc aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt
     7261 gctgaaatgc gtaaagtgct ggcggcaaaa tcggccatca tcgccctgca aattgtacgc
     7321 ggcaatgaaa gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggcttacg
     7381 tgtgatgtcc ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca
     7441 tcatgtttgt gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc
     7501 tggttgccat gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta
     7561 ccgatgagac gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta
     7621 acgtttacaa ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg
     7681 gatccggtgt aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg
     7741 acgccgatca gatcatcgtc gccttacagg atggtcgtgt atttgaagca ttgctggtgg
     7801 gatctgactc tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta
     7861 ccattccaat caatgcacgt cgcgtaccgc atattggcga cgtggtactg gcgatcggta
     7921 acccgtacaa cctcgggcag accattaccc aggggattat tagtgccacg ggtcgaattg
     7981 gtctgaaccc gaccgggcgg caaaacttcc tgcaaaccga tgcttccatt aaccacggta
     8041 actctggcgg cgcgctggtg aactcgctgg gcgaactgat gggcattaac acgctgtcgt
     8101 ttgataagag taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt
     8161 tagcaaccaa aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg
     8221 gcattggcgg acgtgagatc gcaccgctgc acgcacaggg cggtggtata gatcaactgc
     8281 aagggatcgt ggttaatgaa gtgtcacctg acggcccggc ggcgaatgcg ggtattcagg
     8341 tcaacgatct gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg
     8401 atcaggtggc ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata
     8461 agcagttaac gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct
     8521 caaaacaaaa aaccggagtc tgtg
//
LOCUS       DAZPJR010000170         8533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPJR010000170 DAZPJR010000000
VERSION     DAZPJR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.174"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="ACHVB7_003632"
     CDS             complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="ACHVB7_003632"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR1915519.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHS"
     gene            complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="ACHVB7_003633"
     CDS             complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="ACHVB7_003633"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR1915520.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1322..2986)
                     /locus_tag="ACHVB7_003634"
     CDS             complement(1322..2986)
                     /locus_tag="ACHVB7_003634"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR1915521.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3028..3861
                     /locus_tag="ACHVB7_003635"
                     /pseudo
     CDS             3028..3861
                     /locus_tag="ACHVB7_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            3938..5020
                     /gene="lacI"
                     /locus_tag="ACHVB7_003636"
     CDS             3938..5020
                     /gene="lacI"
                     /locus_tag="ACHVB7_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR1915522.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            5143..8217
                     /gene="lacZ"
                     /locus_tag="ACHVB7_003637"
     CDS             5143..8217
                     /gene="lacZ"
                     /locus_tag="ACHVB7_003637"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR1915523.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPHWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            8269..>8533
                     /locus_tag="ACHVB7_003638"
     CDS             8269..>8533
                     /locus_tag="ACHVB7_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1915524.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         1941 a         2390 c         2334 g         1868 t
ORIGIN      
        1 aactatggcc gcccatcgag ttgcccagca ggtggatttt ggcgatatcc agttgatcca
       61 ccacgctttt caggattcgt gcattaagat ccgatcgtga accactatta acgatcgaat
      121 cgcttttgcc ccaacccgga caatccagca ggatcacccg atagcccgcc tctaccagcg
      181 gatcgatatt gcggctgaag ttcgcccagc cagtagcacc cgggccggaa ccatgcagca
      241 ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg gatgcgcagc gttttacccg
      301 cttcttctac attcagaaaa cggctggtgg cggcttcggt ttgtggctga taactcatca
      361 tcttctcctg catattcagt tctctgttct ggcgcttaac gagccaaatc cggcaatcca
      421 ctccgggatt gggcggtaat aacgcccttc gctacgccag ttgccaaatg cagaaatagc
      481 ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac tttccggcaa tggcggaaag
      541 ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc tgctccagca aagtcatgaa
      601 ctggttatcc caaatcgggt tgagcggatg cagagttctc tgatcctcaa caaacttctc
      661 agcggcgcta atcacccttt gctgacgcaa ttcgcgctca ctggcgggta aatctttccc
      721 gctccccaac agacggtcgc gcatatgggc atcggctttc gccagttcgg gcaccggcgg
      781 ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt ttattgagag tgctggtgaa
      841 acgtccaatg gcttcaccca acatgcgggt acgctggaaa ccgggcagcg gcgtggcgac
      901 accgttgatg aacacaggca gaactggcac cttatccagc ccaccgagca ggaactccag
      961 cggctgggcg aacccgtggt ccacctgcat acagtaagaa acggcaagat cgatcccgct
     1021 cttcatgaca gcatgcgcac aggcctccgc cagctccaca ggcacgggca gctctcctgc
     1081 cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct aaacagaacg gtggcatcac
     1141 gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc accaccagtt caggggagaa
     1201 agccgcaata cgctcgcggg cgctggcaat cacgccattg acctcatcga gtacctcttg
     1261 cgccgggtcg acatacccca ccaacggcga gtgggaaaga cagtgaagat aagcgtgcat
     1321 atcaggctac cttttcgaca gaaacgtcgg catcagggcg ggtcagcgtc atcaccgacg
     1381 ccagtttatt cagcgtattg cccagggttt gcggaatggc ggtggcggca acaaagcgat
     1441 ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa ccagctacgc agacgacctt
     1501 gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg tgcggtatga atttgcactt
     1561 ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca ctgctggatt tgctcgtcgc
     1621 tcatccccca cagtggattg catccccagc caattaccgc gaagttcgcg ccgatcgcgt
     1681 tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt cggctgaata aacatcttgc
     1741 cgaccggaga gtgcttcgct tcgccctcac gcaccagcgc accgccgtaa tattgcggca
     1801 tcggcttgaa gcgcatttcg aggaagtagc gttttactgg cggcagataa ttcaacagcc
     1861 aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg cggagccagc acgttgcccg
     1921 ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg acgttcttgt tgataggtat
     1981 cgagcagcgc atcgcgggct ttcccctgga taaccaacgc cagtttccag gcgaggttaa
     2041 aggcgtcgcg cataccactg ttatagccct gcccctgcca caccggcatg atgtgcgcgg
     2101 catcgcccgc cagcagtacg cgatcaatac ggaaacgttg cgccagtcgc gcgttatggg
     2161 tgtagacacg ctggcgaatc aattcaacat tgtccggatt aggcagcact ttgcttaaca
     2221 gcttgcgcat attttgcggc tcacgcagct gctcttcggt ttctcccggc atcaccataa
     2281 attcaaagcg acgtaccgca tgaggcagcg cggcagaaac atacgggcgc accggatcgc
     2341 aacacaaata gatatgcggc gtacttaacg gatcgttggc gatatctacc acaatccact
     2401 gatttggcgc agttttacct tcaaacggca cattcagggt gcgacgaaca aaacttgccc
     2461 caccatcaca ggccaccagc cactgggctt tgatcgtttc ccgctgccct tctgccgttt
     2521 tcaggtgcaa ggtcacttcg tcatcttgct ggctgaaggc ctccagctcg cgggaaaaca
     2581 agcagcgcac attcggaaaa cgcgacaacc cttccagcat caccgcatcg acctgtggct
     2641 gaataaaggc gttacggcgc ggccagccaa attcatcggt cattggctga atatcagcaa
     2701 aacagcggcc ttttggggtg agaaaacgca tcgcgtgcca cggtgtagtg tgcggcagaa
     2761 cattctcgac caggccgacc gactgcatgg tgcgcagcgc ctcgtcatca ataccaatcg
     2821 cacgcgggta gtcgatcaac ttatcgagtt tctccaccac cagcacgtca atgcccatct
     2881 gaccgagata gttcgccatc atcagcccaa ccggaccggc accagcgatc gccacctgaa
     2941 cgctatggtt aacagcaggc tggatgtcag ggtgttgtat tgccatttct gcacaacgtt
     3001 gttttaacta tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc
     3061 gtgcgcggct taacccgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt
     3121 gccagcgtcg ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg
     3181 ctggagacgc tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt
     3241 ctgaccatca aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca
     3301 ctggcggccc cgctgctggg cgatctgttg cgcgaagtgg tatgaccaac agatgtgtcc
     3361 accctggatg ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc
     3421 tttcaccggg caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc
     3481 tggctggcct tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc
     3541 cccggtgatg actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc
     3601 gcgcgcaaag agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc
     3661 tctatcgccg taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat
     3721 atggcgagcg caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg
     3781 gtagcaaaac agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga
     3841 agcggcgtgc atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg
     3901 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
     3961 tgtcgcagag tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag
     4021 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     4081 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     4141 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     4201 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     4261 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     4321 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     4381 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     4441 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     4501 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     4561 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     4621 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     4681 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     4741 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     4801 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     4861 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     4921 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5101 gtgaaattgt gagcggataa caatttcaca caggaaacag ctatgatcat gattacggat
     5161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     5341 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     5401 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
     5461 cccattacgg tcaatccgcc ttttgttccc gcggagaatc cgacgggttg ttactcgctc
     5521 acatttaata ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggt
     5581 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagc
     5641 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggaaaaaa ccgcctcgcg
     5701 gtgatggtgc tgcgctggag tgacggtagt tatctggaag atcaggatat gtggcgaatg
     5761 agcggcattt tccgtgacgt ctcgttgctg cacaaaccga gcacacaaat cagcgatttc
     5821 catgttgcca ctcactttaa tgatgatttc agccgcgcgg tactggaggc agaagttcag
     5881 atgtacggcg agctgcgcga tgagctgcgg gtgacggttt ctttgtggca gggtgaaacg
     5941 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
     6001 gccgatcgcg tcacgctacg tctgaacgtc gaaaacccgg cgctgtggag cgccgaaatc
     6061 ccgaatctct atcgtgcggt ggttgaactg cacaccgacg acggcacgct gattgaagca
     6121 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     6181 ggcaagccgt tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag
     6241 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     6301 gccgtgcgtt gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgcttc
     6361 ggcttgtacg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     6421 ctgaccgatg atccgcactg gttacccgcg atgagcgaac gcgtaacgcg aatggtgcag
     6481 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgagtc aggccacggc
     6541 gctaatcacg acgcactcta tcgctggatc aaatctgtcg atccatcccg cccggtgcag
     6601 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     6661 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     6721 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     6781 cttggcggct tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
     6841 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaatgg caatccgtgg
     6901 tcggcttacg gcggtgattt tggcgataca ccgaacgatc gccagttctg catgaacggt
     6961 ctggtctttg ccgaccgcac gccgcatcca gcgctgacag aagcaaaaca ccagcagcag
     7021 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     7081 catagcgata acgagcttct gcactggtcg gtggcgttgg atggcaagcc gctggcaagc
     7141 ggtgaaatgc ctctggatgt tgctccacaa gataaacagt tgattgaact gcctgaacta
     7201 ccgcagccgg agagcgccgg acaactctgg cttacggtac acgtagtgca accgaacgcg
     7261 accgcatggt cagaagccgg acacatcagc gcctggcagc agtggcgtct ggcggaaaac
     7321 ctcagcgtga cactccccgc cgcgccccac gccatcccgc aactgaccac cagcgaaacg
     7381 gatttttgca tcgagctggg taataaacgt tggcaattta accgccagtc aggctttctt
     7441 tcacagatgt ggattggcgc tgaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     7501 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga cccgaacgcc
     7561 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     7621 acggcagata cacttgccga cgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     7681 ggaaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     7741 attaccgttg atgttgaagt agcgagcgat acaccgcatc cggctcggat tggcctgacc
     7801 tgccagctgg cgcaggtagc agaacgggta aactggctcg gattagggcc gcaagaaaac
     7861 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     7921 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     7981 tatggcccac accagtggcg cggcgacttc cagttcaata tcagtcgcta cagccaacaa
     8041 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     8101 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtttcagcg
     8161 gaattccagc ttagcgccgg tagctaccat taccagttgg tctggtgtca aaaataataa
     8221 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     8281 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatgggg
     8341 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     8401 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     8461 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
     8521 gtgatgtttg cgc
//
LOCUS       DAZPJR010000171         8506 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPJR010000171 DAZPJR010000000
VERSION     DAZPJR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.175"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(257..333)
                     /locus_tag="ACHVB7_003639"
     tRNA            complement(257..333)
                     /locus_tag="ACHVB7_003639"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(297..299),aa:Asp,seq:gtc)
     gene            complement(466..1197)
                     /gene="dnaQ"
                     /locus_tag="ACHVB7_003640"
     CDS             complement(466..1197)
                     /gene="dnaQ"
                     /locus_tag="ACHVB7_003640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR1915525.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1262..1729
                     /gene="rnhA"
                     /locus_tag="ACHVB7_003641"
     CDS             1262..1729
                     /gene="rnhA"
                     /locus_tag="ACHVB7_003641"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1915526.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1726..2448)
                     /locus_tag="ACHVB7_003642"
     CDS             complement(1726..2448)
                     /locus_tag="ACHVB7_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1915527.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2482..3237
                     /gene="gloB"
                     /locus_tag="ACHVB7_003643"
     CDS             2482..3237
                     /gene="gloB"
                     /locus_tag="ACHVB7_003643"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR1915528.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3309..4667
                     /gene="mltD"
                     /locus_tag="ACHVB7_003644"
     CDS             3309..4667
                     /gene="mltD"
                     /locus_tag="ACHVB7_003644"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR1915529.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4716..5339)
                     /locus_tag="ACHVB7_003645"
     CDS             complement(4716..5339)
                     /locus_tag="ACHVB7_003645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1915530.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5343..6143)
                     /locus_tag="ACHVB7_003646"
     CDS             complement(5343..6143)
                     /locus_tag="ACHVB7_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1915531.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6384..7298
                     /gene="yafC"
                     /locus_tag="ACHVB7_003647"
     CDS             6384..7298
                     /gene="yafC"
                     /locus_tag="ACHVB7_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR1915532.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7295..8098)
                     /gene="dkgB"
                     /locus_tag="ACHVB7_003648"
     CDS             complement(7295..8098)
                     /gene="dkgB"
                     /locus_tag="ACHVB7_003648"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR1915533.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8261..8337)
                     /locus_tag="ACHVB7_003649"
     tRNA            complement(8261..8337)
                     /locus_tag="ACHVB7_003649"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8301..8303),aa:Asp,seq:gtc)
BASE COUNT         2303 a         2028 c         2037 g         2138 t
ORIGIN      
        1 aatcagaaca aacaaaaata acagtatata taaattcatt aaatcaatag aaaaatgaaa
       61 atcataacat tcaaatatat ttaaattgaa tgatttgata gtatgattaa atattaatga
      121 tatggtctac catatgtgat taacacattt acagcataac aaaaccatag accgaagaaa
      181 tagcgggatt taaatgcaaa aaaaccgcaa aattaaaaat cttgcggttc tctgaactca
      241 ttttcatgag tgaatttggc ggaacggacg ggactcgaac ccgcgccccc ctgcgtgaca
      301 ggcaggtatt ctaaccgact gaactaccgc tccgcgttgt gttccgttgg gaacgaggcg
      361 aatattacgg attgcctcac ccttcgtcaa cggtttttct catcttttga atcgtttgct
      421 gcaaaaatcg cccaagccgc tatttttagc gccttttaca ggtatttatg cccgccagag
      481 gcagcttccg cccttcttct ccaccagatc aagacgggct tcatgagctg caagctcttc
      541 atctgtcgca aaaacaacgc gtaacttact tgcctgacgt acaatgcgct gaattgttgc
      601 ttcaccttgt tgctgctgtg tctctccttc catcgcaaaa gccatcgacg tttgaccacc
      661 ggtcatcgcc agataaactt ccgcaaggat ctgtgcatcg agtaatgccc cgtgtagcgt
      721 tcgtttactg ttatctattt cgtagcgggc acataacgca tcgaggctgt tgcgcttacc
      781 aggaaacatt ttcctcgcca ccgcaaggct atcggtgacc ttacagaagg tattggtctt
      841 cggaatatcg cgcttaagca acgaaaactc gtagtccata aagccgatat cgaacgctgc
      901 gttatggatc accaactccg cgccgcggat atagtccatg aactcatcgg ctacttcggc
      961 aaacgtgggc ttatcgagca aaaattcatc ggcaatacca tgtacgccaa aggcttccgg
     1021 atccaccagc cgatcgggtt tgagataaac atggaagtta ttgcccgtca ggcgacggtt
     1081 caccacttca acggcaccaa tctcaatgat cttgtggcct tcatagtgcg caccaatctg
     1141 gttcataccg gtggtttcgg tatcgagaac gatctggcgt gtaattgcag tgctcatagc
     1201 ggtcatttat gtcagactta tcgttttaca gttcgattca attacaggaa gtctaccaga
     1261 gatgcttaaa caggtagaaa ttttcaccga tggttcgtgt ctgggcaatc caggacctgg
     1321 gggttacggc gctattttac gctatcgcgg acgcgagaaa acctttagcg caggctacac
     1381 ccgcaccacc aacaaccgta tggagttgat ggccgctatt gtcgcgctgg aggcgttaaa
     1441 agaacattgc gaagtcattt tgagtaccga cagccagtat gtccgccagg gtatcaccca
     1501 gtggatccat aactggaaaa aacgtggctg gaaaaccgca gacaaaaaac cagtaaaaaa
     1561 tgtcgatctc tggcaacgtc ttgatgctgc cctagggcaa catcaaatca aatgggaatg
     1621 ggttaaaggc catgccggac acccggaaaa cgaacgctgt gatgaactgg ctcgtgctgc
     1681 ggcaatgaat cccacactgg aagatacggg ctaccaagtc gaagtttaag cttgtggttt
     1741 acgacattgc cgggtggctc caaccgcctg gcgaattcgt ggcttgtttt tactctgttt
     1801 catcgggttt agcgttaaag gaatagtccg tttccgggca acaataagtt gtaagcaacc
     1861 aagcgcagga atatgcgcat tcaatagttt tcctccgtgt ttgttccacg ggagaacgtg
     1921 gaaacggctg gcgtgcagca cttcaaaatt caacaaagag agccagtcca gttgccgcat
     1981 cagagtaaac atccggctgt tatagggcga ggtttttcgc aataccggca caagtttgcg
     2041 taatcccatt aaactaatgg gattgaagcc actaatcacc agccagccat catcaatcaa
     2101 tacccgatcg gtttcacgca ataaacgatg cggatcggtg caccacggca atgtatgtgc
     2161 cagtagacaa acatcaacgg atttatcggc aaaaggaaga tgaagtggat ccgcctgcac
     2221 ctggacgggc attccttgcg cagaaacatt cacttgatga gaaaccgcgc acgcttcgca
     2281 attgatttct gcgcttaaat tgccaatctt aagcagatga aaaccataca ttttagtgaa
     2341 ccacgggttg agctggcgct ccagcgcctt gcgataaagc tctccccagg gcaaatcgcc
     2401 ccagcgatca ggagccacga cagtttgagg gactcttgcc ggtttcatca caaccttccg
     2461 tttcacacta agaggtaatc tatgaatctt aacagtattc ccgcctttga tgacaattac
     2521 atctgggttt tgaatgatga agcaggtcgc tgcctgattg tcgatcccgg agacgcagag
     2581 ccagtattaa acgccattgc cgccaataac tggcaaccgg aggccatatt tctcacccac
     2641 catcatcacg atcacgttgg cggcgtaaaa gaactggtgg aaaagtttcc gcaaattgtg
     2701 gtgtatggtc cacaagagac acaagataag ggaacaacac aggtagtcaa agatggcgaa
     2761 actgccttcg ttttggggca tgaatttagt gtaattgcca caccgggtca cactttagga
     2821 catatctgtt acttcagtaa accttatcta ttttgcggcg acacgctgtt ttctggtggg
     2881 tgtggtaggt tgtttgaagg gacagcatca caaatgtatc aatcacttaa taagttaagt
     2941 gcgttacctg acgatacatt ggtatgttgt gctcatgaat atacgttatc aaatatgaag
     3001 tttgctttga gtattcttcc gcacgatttg tccataaatg attattatcg taaagttaag
     3061 gagttacggg caaaaaatca aataacacta cccgtaattc tgaaaaatga gcggcaaatt
     3121 aatgtttttt taagaacgga agatattgat ttaattaatg taattaatga agaaacatta
     3181 ttgcaacaac ctgaagagcg ttttgcatgg ttaaggtcaa agaaagatag gttctgataa
     3241 aactttcttg tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa gcaactattg
     3301 acacacacat gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg ggttgccaga
     3361 gtaccggcaa cgttcaacag cacgcacaga gcctttctgc agctggtcaa ggggaagcag
     3421 caaagtttac aagtcaggca cgatggatgg acgatgggac gtctatcgcg ccagatggtg
     3481 acttgtgggc tttcattggc gacgagctaa agatgggaat tccggaaaat gaccggattc
     3541 gcgaacagaa acagaaatat ttacgcaata agagctatct ccacgatgta actttacggg
     3601 cagagccgta tatgtactgg atagcagggc aagttaaaaa acgtaacatg cctatggaac
     3661 tggtactact acccatagtg gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg
     3721 ccgcaggcat ctggcagatc attccgagca cggggcgcaa ttatggtttg aaacagaccc
     3781 gcaattatga cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg aatatgatgc
     3841 agcgtctgaa caaaatgttt gacggcgact ggcttctgac cgtagcggct tataacagcg
     3901 gcgaaggtcg ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact
     3961 tctggtcgtt accgttgccg caggaaacga agcagtacgt gcctaaaatg ctggcattga
     4021 gtgatattct caaaaacagc aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc
     4081 gtgctctggc gcgtgtgcac ctgagtagcc cggttgaaat ggcgaaggtt gcagatatgg
     4141 cggggatttc cgtcagcaag ctgaagacat tcaacgctgg cgtgaaaggc tccacgctgg
     4201 gcgcaagtgg gccgcagtac gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat
     4261 ctctcgcttc aggcgaaatt gctgctgtac agtcgacgct ggttgccgac aatacgccgc
     4321 ttaacagccg tgtttacacc gtacgctctg gcgacacgct ttcaagcatc gcttcacgtc
     4381 tcggcgtaag caccaaagat ttgcagcagt ggaacaaact gcgcggatct aagctgaagc
     4441 caggccaaag tttgacgatt ggtgcaggca gtagcgcaca gcggttggca aacaacagcg
     4501 atagcattac gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct aaacgccacg
     4561 gcgtgaacat caaagatgtg atgcgctgga acagcgacac tgcgaatctg caaccaggcg
     4621 ataagctgac gttgtttgtg aaaaacaaca acatgccaga ttcctgacaa accagataat
     4681 aaaaaaggca ccgattcccc cggtgccttt tttatttatg ccgctttatg tgcttctacc
     4741 atgatgatat cactggtgaa agagccatca gtctgcaagg aaaaatacgt tctcacctct
     4801 gtcgatgcgc tctgttggta aatgcgaata gcgtctacta acgcttctgg cgtacgcatt
     4861 ctcacgaccc atgaagaaaa ttccagcggt aacttatctg taattaaatt atcaactatc
     4921 agattggctt cattgattaa cgtcaaccac gcaccgctgg cgtagtttcg tacgtgagag
     4981 gtatcgcgta atgcttctac cgcctgtaac cagatgtcgc gcactgggtg acccggagac
     5041 attacatcca tcacaatcag cctaccgcca ggtttcaata tcctattcac ttctcgcagt
     5101 gctgcaccaa catcatgcca gtgatgggca gaataacggc tgataacaat atcaaatgcg
     5161 ttatcggcaa atggcagact tgcggcatat ccctggcggg tggtgatatt tttcagttgc
     5221 cgggcttcgg cagcttgtgc cacgacatcc agcatgtggg cagataagtc atacgccacc
     5281 accgcgctca cgttttgcgc agcgccaaac gttggtctgc cctgcggcag acctgacata
     5341 ccttatttat caggcttgcc gggactgaat tcaacgagta gcggattgtg atcggaagcg
     5401 cgcgtaacca gtacagaagc ttcactgacg ttcagaccac ggtagaaaac aaaatcgagc
     5461 gggcgaccaa acgcccgacg gcgctgatca tcggtaaaac gcacctggcg cagcgacatt
     5521 tcccgcgcaa agcgatataa cgcgttcatc cttctacggc tccaggcatt gaaatctccc
     5581 gccataatga ccgggccgct gtggtgagct atctgatcac caataggaag taactgctta
     5641 ctatagacat ccacgcccag actgaagttg acggcgtgta tattaaccac catcaacagg
     5701 cgggtgtcag gtaatggata gaccgtcacc agtgccgact tcgccagacg caaaatgggt
     5761 tctcgttcac gtaacgggca gcaatacact ggatgtgccg ccgaaagggt cattacgcca
     5821 gaaggatgtt gtggcagcac gaaagcgggt acctgatcgg cggcaagata gttagcggtc
     5881 gcaaactgta ccaactctgg cgttgtttgc gcttcctgca ataacaccag atgtgcgtct
     5941 ttgccgtaat tctttaatac cgacaaccat tcagcgcgtt gctgtttgta tatgttccac
     6001 accaggatcc gaatacgctc ttcggtactt aacggttctc cgggtggtaa tgcctggccg
     6061 atgctcgcaa aagaccccgg cggtaagatc ctctccgcag gttgtccggc aacatagcgc
     6121 atggcatagg tgttttttcg cacttttgac tttgaaacct ctattacgtc aacccttccg
     6181 cactcgggag ggttctactg ttatagggat tttagctcac tctttcaacg agcaattgta
     6241 tattgttatg taagcaagtg cttacaccgg tcagaaattt gctcattatt gagaacatta
     6301 aatcggatga catatcacgc atcctgatgg tttaatgaca aataaattca aaatatctca
     6361 tctattttgc aacaggagca aaaatgaaag ccacgtcgga agaactcgcc atttttgttt
     6421 cggtcgtcga aagcggcagc tttagccggg cagcggaaca attagggcaa gcaaactcag
     6481 cggtaagccg ggcggtgaaa aagctggaaa tgaaacttgg cgttagcctg cttaatcgga
     6541 ccacgcggca acttagcctg acggaagaag gcgagcgtta ttttcgtcgc gtacagtcaa
     6601 ttttgcagga gatggcagcg gcagaatcag aaattatgga gacgcgtaat acaccgcgtg
     6661 gactgttacg gatcgatgcc gcaactccag tggtgctgca ctttctgatg ccgttaatta
     6721 agcctttccg tgaacgctat ccggaagtca ctttatcgct agtctcctcc gaaacgatta
     6781 ttaatttgat cgaaagaaaa gtggatgtcg cgatacgcgc tggtacgtta acggactcca
     6841 gcttacgtgc caggccgtta tttaacagtt atcgaaaaat tatcgcctcc cccgattata
     6901 tttcccgcta cgggaagcca gaaacgatcg acgatttaaa gcaacatgtt tgcctgggat
     6961 tcactgaacc cgcttccctc aatacctggc cgatagcctg tagtgatgga caattacatg
     7021 aggtgaagta cggtttgtca tccaatagtg gggaaacact gaaacagctt tgcctgagtg
     7081 gcaacgggat tgcgtgtttg tccgactaca tgatcgacaa agaaatcgct cgcggagaat
     7141 tggtggagtt aatggcagat aaagtgttgc cagtggaaat gccattcagt gccgtctatt
     7201 acagcgaccg tgcggtaagt acgcgcatcc gggcttttat cgatttcctt agcgagcatg
     7261 taaaaacagc tcccggagga gctgtcagag aggcttaatc ccattcagga gccagacctt
     7321 ccgggctaac caggcggtcg ttgcaatcca gtgcggcgat cgctttttta tcttcggcat
     7381 caagctgtaa attttgtgcc ttaagattac tttccaggtt tttacgttta gtagaagaag
     7441 gaattactga gtaaccttcc cccatagccc acgccagaat cacttgtgcc ggagtcgcat
     7501 tgtgtttagc tgcaatacga gcaataacct catctttcag tgccttacca taagccagcg
     7561 tcatatagga agtgatatga ataccgtgct gtttagccca ggcaaccact ttacggtttt
     7621 gcagataagg agagagttca atctggttgg tagcgatgtt ttcagcgcca acagcagcaa
     7681 tcgctttttc catcaacggg atcgtgaagt tggaaatacc gatctcacgt gtcagacctt
     7741 gttttttggc ctccagcagc gcctgcataa actcttcaac agagacttca tcgtttggtg
     7801 acggccagtg gattagcgtc agatcaacat aatcggtacg caatttttgc aggctctctt
     7861 ttagactcgg gatcaatttg tctttgctga gattttcaat ccagatttta gtggtgatgt
     7921 agagttcatg acgtggcacg ccactttctg caatcgcctg acctactgcg gcttcgttat
     7981 catagatttg tgcagtatca attgcgcgat aaccaagttc aagcgccgtt ttcacagatg
     8041 aaataacaac gtcgtctttg agacggaaag tacctaaacc aaatgcaggg atagccataa
     8101 tatgcctctt ttaatgctat tttcgttaac tgaggtgatt atgggcttca gactgttgcg
     8161 gaaaaagagc ataaaatgca gaggattttt gcgattctgg caataataga tatacagacg
     8221 aaaaaaaacc tcgagcgtta actcgaggct tcttaataag tggcggaacg gacgggactc
     8281 gaacccgcga ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccacc
     8341 gaattctttt acaaccaccg gttttatgac cggcttactg cttaatttga tgcctggcag
     8401 tttatgaatc acttcgggct gttgcttcgc aacgttcaaa agctttcgct tttgtcctac
     8461 tctcttatcg agtagtgaac tgcctcaaag gtcagtgcca ttcttc
//
LOCUS       DAZPJR010000172         8401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPJR010000172 DAZPJR010000000
VERSION     DAZPJR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.176"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHVB7_003650"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHVB7_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR1915534.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(1360..1518)
                     /locus_tag="ACHVB7_003651"
     CDS             complement(1360..1518)
                     /locus_tag="ACHVB7_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1915535.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            2089..2238
                     /locus_tag="ACHVB7_003652"
     CDS             2089..2238
                     /locus_tag="ACHVB7_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915536.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="ACHVB7_003653"
     CDS             complement(3005..3445)
                     /locus_tag="ACHVB7_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR1915537.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="ACHVB7_003654"
     CDS             3663..4052
                     /locus_tag="ACHVB7_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915538.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4284
                     /locus_tag="ACHVB7_003655"
                     /pseudo
     CDS             4096..4284
                     /locus_tag="ACHVB7_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="ACHVB7_003656"
     CDS             complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="ACHVB7_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR1915539.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            5556..6206
                     /gene="tetR(A)"
                     /locus_tag="ACHVB7_003657"
     CDS             5556..6206
                     /gene="tetR(A)"
                     /locus_tag="ACHVB7_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR1915540.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(6238..6480)
                     /locus_tag="ACHVB7_003658"
     CDS             complement(6238..6480)
                     /locus_tag="ACHVB7_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFR1915541.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(6786..7622)
                     /locus_tag="ACHVB7_003659"
     CDS             complement(6786..7622)
                     /locus_tag="ACHVB7_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR1915542.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(7622..>8401)
                     /locus_tag="ACHVB7_003660"
                     /note="aph(3'')-Ib"
     CDS             complement(7622..>8401)
                     /locus_tag="ACHVB7_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR1915543.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1889 a         2322 c         2332 g         1858 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaaagcccg cgacgcagcg ccggcaggca gagcaagtag
     4321 agggcagcgc ctgcaatcca tgcccacccg ttccacgttg ttatagaagc cgcatagatc
     4381 gccgtgaaga ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc cagtgagcct
     4441 tgcagctgcc cctgacgttc ctcatccacc tgcctggaca acattgcttg cagcgccggc
     4501 attccgatgc cacccgaagc aagcaggacc atgatcggga acgccatcca tccccgtgtc
     4561 gcgaaggcaa gcaggatgta gcctgtgccg tcggcaatca ttccgagcat gagtgcccgc
     4621 ctttcgccga gccgggcggc tacagggccg gtgatcattg cctgggcgag tgaatgcaga
     4681 atgccaaatg cggcaagcga aatgccgatc gtggtcgcgt cccagtgaaa gcgatcctcg
     4741 ccgaaaatga cccaaagcgc ggccggcacc tgtccgacaa gttgcatgat gaagaagacc
     4801 gccatcaggg cggcgacgac ggtcatgccc cgggcccacc ggaacgaagc gagcgggttg
     4861 agagcctccc ggcgtaacgg ccggcgttcg cctttgtgcg actccggcaa aaggaaacag
     4921 cccgtcagga aattgaggcc gttcaaggct gccgcggcga agaacggagc gtggggggag
     4981 aaaccgccca tcagcccacc gagcacaggt cccgcgacca tcccgaaccc gaaacaggcg
     5041 ctcatgaagc cgaagtgccg cgcgcgctca tcgccatcag tgatatcggc aatataagcg
     5101 ccggctaccg ccccagtcgc cccggtgatg ccggccacga tccgcccgat atagagaacc
     5161 caaaggaaag gcgccgtcgc catgatggcg tagtcgacag cagcgccggc cagcgagacg
     5221 agcaagaccg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca
     5281 aattgcatca acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag
     5341 tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg
     5401 tcgagcgcga cagtgctcag aattacgatc aggggtctgt tgggtttcac gtctggcctc
     5461 cggaccagcc tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg
     5521 gacatattat gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag
     5581 tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca
     5641 aactggcgga acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc
     5701 gggcgctgct cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc
     5761 cgagagccga cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg
     5821 cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc
     5881 agatggaaac ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg
     5941 acgccgtcaa tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc
     6001 aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc
     6061 cgctgttgcg ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc
     6121 agggactcgc ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg
     6181 aaggaccgag aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta
     6241 cagcagagcc atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca
     6301 gcccgctacg ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc
     6361 ctctctggcg gccttctggc gctcctgctg cggcgtccgc tcgtgggccg tggcgcgggt
     6421 ccgcgcgccg gcctcgtgcg cctggcgctc gcgggcgagg tccagggcgg ccgtcttcac
     6481 gttctgcctt gcgcagatga gatagatcta gcgtggactc aaggctctcg cgaatggctc
     6541 gcgttggaaa ctttcattga cacttgaggg gcaccgcagg gaaattctcg tccttgcgag
     6601 aaccggctat gtcgtgctgc gcatcgagcc tgcgcccttg gcttgtctcg cccctctccg
     6661 cgtcgctacg gggcttccag gggtcgtttg cgggaagggg cggaatccta cgctaaggct
     6721 ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt
     6781 gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt
     6841 tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg
     6901 tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca
     6961 atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg
     7021 gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg
     7081 gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg
     7141 ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt
     7201 tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca
     7261 gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg
     7321 gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat
     7381 tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc
     7441 cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc
     7501 ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg
     7561 tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac
     7621 atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg
     7681 cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg
     7741 cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc
     7801 cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt
     7861 tcggcatgca gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca
     7921 ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt
     7981 ccggtaagaa gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca
     8041 ttcgcgacag cctgcgctca aactgacatt gatcaaccga taggctgtga acagcgccaa
     8101 gttgctgccc cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg
     8161 gtactcccgg aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga
     8221 tgacctcggg gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag
     8281 cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc
     8341 gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgattcac
     8401 c
//
LOCUS       DAZPJR010000173         8332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPJR010000173 DAZPJR010000000
VERSION     DAZPJR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.177"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            154..765
                     /gene="ruvA"
                     /locus_tag="ACHVB7_003661"
     CDS             154..765
                     /gene="ruvA"
                     /locus_tag="ACHVB7_003661"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR1915544.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            774..1784
                     /gene="ruvB"
                     /locus_tag="ACHVB7_003662"
     CDS             774..1784
                     /gene="ruvB"
                     /locus_tag="ACHVB7_003662"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR1915545.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1931..2716)
                     /gene="znuB"
                     /locus_tag="ACHVB7_003663"
     CDS             complement(1931..2716)
                     /gene="znuB"
                     /locus_tag="ACHVB7_003663"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR1915546.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2713..3468)
                     /gene="znuC"
                     /locus_tag="ACHVB7_003664"
     CDS             complement(2713..3468)
                     /gene="znuC"
                     /locus_tag="ACHVB7_003664"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR1915547.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3547..4479
                     /gene="znuA"
                     /locus_tag="ACHVB7_003665"
     CDS             3547..4479
                     /gene="znuA"
                     /locus_tag="ACHVB7_003665"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR1915548.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4495..5817
                     /gene="mepM"
                     /locus_tag="ACHVB7_003666"
     CDS             4495..5817
                     /gene="mepM"
                     /locus_tag="ACHVB7_003666"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR1915549.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5937..6908
                     /gene="lpxM"
                     /locus_tag="ACHVB7_003667"
                     /gene_synonym="msbB"
     CDS             5937..6908
                     /gene="lpxM"
                     /locus_tag="ACHVB7_003667"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1915550.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6996..8332)
                     /locus_tag="ACHVB7_003668"
                     /pseudo
     CDS             complement(6996..8332)
                     /locus_tag="ACHVB7_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2233 a         1971 c         2068 g         2060 t
ORIGIN      
        1 ttgcttccaa tataacctgt agaataaatt atactgtgcc atttttcagt tcatcgagac
       61 acctcgcaag ttttcttcat ccttcgctgg atatctatcc agcatttttt tatcatacag
      121 cattatcttt gattcattac gcaggagcgt catgtgatag gcagactcag aggcatcatc
      181 attgaaaaac aacccccgct ggtgttaatt gaagtgggcg gcgtaggcta tgaagtgcat
      241 atgccgatga cctgttttta tgaactccct gaagcgggtc aggaagcgat cgttttcacc
      301 cactttgtgg tgcgtgaaga cgcgcaactg ctgtacggtt ttaacaataa acaagagcgc
      361 acgttgttta aagagttaat caaaaccaac ggcgtcggcc cgaagttggc gctggcgatc
      421 ctctccggaa tgtcagcgca gcagttcgtt aatgccgttg aacgtgaaga agtgggagca
      481 ctggtgaaac tgccgggcat tggtaaaaaa accgccgagc gtttaattgt cgaaatgaaa
      541 gaccgattta aaggtttgca cggcgatctc tttacgccag ccgccgatct ggtgctcacg
      601 tcgcctgcca gcccggcaac cgacgatgct gaacaggaag cggttgccgc gctggtggcg
      661 ctgggctata aaccacaaga agcaagccgc atggtgagca aaatcgctcg cactgacgcc
      721 agcagtgaaa cattaattcg cgaagcccta cgcgccgcgt tatgaggtaa aggatgattg
      781 aagcagaccg tctgatttct gccggtacca ctttgccgga agatggggca gatcgcgcca
      841 ttcgccctaa attactggaa gagtatgttg gtcagccgca ggttcgttca cagatggaga
      901 ttttcatcaa agcagcgaaa ctgcgcggcg atgccctcga tcatctgttg atttttggtc
      961 ctccggggtt gggtaaaact acgcttgcca acattgtcgc caatgaaatg ggcgttaatt
     1021 tacgcacgac ttctggtccg gtgctggaaa aggcgggcga tctggcagcg atgctcacta
     1081 accttgaacc gcatgacgtg ctgtttattg atgagatcca ccgtctatcg ccagttgttg
     1141 aagaagtgct gtacccggca atggaagact accaactgga tatcatgatt ggtgaaggtc
     1201 cggcggcacg ctccattaaa attgatttgc cgccgtttac cctgattggt gcaaccacgc
     1261 gcgcaggttc gctgacatca ccgttgcgcg accgttttgg tattgtgcaa cgtctggagt
     1321 tttatcaggt gccggatctg caatatatcg tcagtcgcag cgcacgcttt atggggcttg
     1381 agatgagtga tgacggcgcg ctggaagttg ctcgtcgcgc tcgcggtacg ccacgtattg
     1441 ccaaccgtct gctgcgtcga gtgcgtgatt tcgccgaagt gaagcacgat ggcaccatct
     1501 cggcagatat cgctgctcag gcgctggata tgttgaatgt cgatgctgaa ggtttcgatt
     1561 atatggaccg caaattgttg ctggcggtaa tcgataagtt ctttggtggg ccggtaggtc
     1621 tggataacct ggcggcagcc attggcgaag aacgtgaaac cattgaggat gtgctggagc
     1681 cttatctgat tcagcaaggc tttttgcagc gtacaccgcg tgggcgtatg gcgacgacgc
     1741 gggcgtggaa tcactttggc ataacgccgc cagaaatgcc gtaagtcgga ttggtcgctc
     1801 gcaccgcatc cgacattggc agtgtgcctg atgcgacgct attcgcgtct tatccggcct
     1861 accagagata gcgaaccgta ggtcggataa ggcgctcgcg ccgcatccga caaatgtgtt
     1921 cagcgacaga ttagctggcc tgctttttca tcatactgat aataaataac agtgcagcac
     1981 atagcactac cgacggaccc gccggcgtat cgtaaaacgc ggaaaaggtt aaaccgccag
     2041 ttactgccac catccccacc aaaacagcga caccagccat ctgttccggc gtacgggcaa
     2101 agcgacgcgc agtagctgca ggaataatca gcaacgaagt aataatcaac gcaccgacaa
     2161 atttcatcgc tacaccaatc gtcaatgccg tcaccagcat caacaacaat ttcacgcgct
     2221 gtaatttcac accatcaaca aacgccagat ccgggctaat cgtcatcgac agcaaattgc
     2281 gccattgcca gaacaaaata gccaccacga tgaccacgcc aatcgcaata gagatgagat
     2341 cttctggcgt cactgccagc aaatcaccga acaggtaagc catcaaatca acacgaatat
     2401 tagacatcag actaacgacc accaggccca gcgacagggc actgtgcgcc ataatcccta
     2461 ataacgtgtc gatcgccagc tgtggacgct tctccagcca taccagaccg cccgccagca
     2521 gcagcgtaac ggcaatcacc gcatagaatg gattcacgtc cagcaacaaa ccaaacgcga
     2581 caccaagtaa tgaggcatga gccagcgtat caccgaaata agacatacga cgccagacta
     2641 caaacgaacc cagcggaccc gcggcacagg cgagcatgat cccggctaac caaccgggaa
     2701 ataataattc aatcatgagc gatcatttcc ccgacgcaaa acaattcgtc cctgtaaatc
     2761 gtgacgatgg ttatgatgat ggcgatagat acccagttgt tcagcaccac gaggaccaaa
     2821 cattgaaata aactccggat gcagggaaac aacttccggt gtgccggaac aacaaatgtg
     2881 gtgattcagg cacagcactt catcggtttt tgccatcacc agatgcagat cgtgagaaac
     2941 cattaaaacg ccacaatcca gttcacgacg cagttggtca ataaggtcat ataacgccac
     3001 ctggccattt acatccacgc cttgagtggg ttcatccagc actaataatt gcgggcgatt
     3061 taacaatgct cgcgctaaca gtacacgctg cgtttcgcca ccagagagct tttgcatcgg
     3121 tgcgttaatc aggtgcccgg cctggacacg tttcagtgca ggcaaaatat cttctttatg
     3181 tgtaccaggg cgtaagcgta aaaaacggtt tacggtcagt ggcaacgtgg tgtcgagata
     3241 cagcttctgc ggtacatagc cgatgcgcag ttttccgttg cgcttgataa ccccttcatc
     3301 gggtgttacc agcccgagca ctacccgtac cagtgtcgac ttacctgcgc catttggccc
     3361 aagtaaagtc aaaatttttc caggtttaag ttccagcgac acatcagaga ggacgcggcg
     3421 ttggccaaaa gaaaccgaga cattttccaa ggaaaccaga cttgtcatgt taattttagt
     3481 cttgcagtag tcatgaaatg ttataatatc acacttctca tattcattac gatgattggt
     3541 cgcattatgt tacataaaaa aacgcttctt ttcgcagcat tatccgccgc tctctgggga
     3601 ggtgcaacac aggccgcaga tgccgccgtt gtcgcttcgc ttaaacccgt tgggttcatc
     3661 gcttctgcca ttgctgatgg ggtaacagaa acagaggttt tacttcctga cggcgcttca
     3721 gaacatgatt attcactgcg cccatcggat gtaaaacgct tacagaacgc ggacttagtc
     3781 gtttgggttg gcccggagat ggaagcgttt atgcaaaaac cggtaagcaa attaccagga
     3841 gcgaagcagg taacgattgc gcagcttgaa gatgtgaaac cgctactgat gaaaagtatt
     3901 cacggcgatg atgatgatca cgaccacgcg gaaaaaagtg acgaagatca ccatcacggc
     3961 gatttcaaca tgcatctttg gctttcccca gagatagcgc gggctacagc ggttgcaatc
     4021 catggaaaat tagtggaact tatgccgcaa agtcgagcca aacttgacgc caacctgaag
     4081 gattttgagg cacaattagc ctcaaccgaa acgcaggttg gtaacgagct cgcgccgctc
     4141 aaggggaaag gttatttcgt ttttcacgat gcttacggct atttcgaaaa acagttcgga
     4201 ctgacaccgc ttggtcattt taccgttaac cctgagattc aacctggcgc gcagcgttta
     4261 catgaaataa gaacacagtt ggttgagcaa aaagcaacct gcgtttttgc tgagccacag
     4321 ttcaggccag cggtcgttga gagcgtcgca cgagggacat ccgttcgtat gggaacgttg
     4381 gatcccctgg ggacgaatat caaactgggt aaaacaagct attcagaatt cctgagtcaa
     4441 ttagccaacc agtatgcgag ctgcctgaaa ggagattaat gaggaagtga ttacgtgcaa
     4501 cagatagccc gctctgtcgc cctggcgttt aataatttac cgcgaccaca ccgcgttatg
     4561 ttggggtcgc tcaccgttct tactctggcc gtcgctgtct ggcggcccta tgtttatcac
     4621 cgcgatgcca cgccaattgt caaaaccatt gagctggaac agaacgaaat tcgttcgctc
     4681 ttacctgaag ccagtgagcc gattgatcaa gctgcacaag aagatgaagc cattccccag
     4741 gacgaactgg atgacaaaat cgccggtgaa gcgggcgtgc atgaatatgt tgtttccact
     4801 ggcgatacgc taagcagcat tctcaatcag tatggtattg atatgggtga tatcacccaa
     4861 ctggctgcgg ccgacaaaga attgcgtaac ctgaaaatcg gtcaacaact ctcctggaca
     4921 ttaaccgcgg acggcgaact gcagcgcctc acctgggaag tgtctcgtcg tgaaacccga
     4981 acctatgacc gtactgccgc taacggtttt aaaatgacca gcgaaatgca gcaaggagag
     5041 tgggttaaca atctgctgaa aggtaccgtc ggggggagct ttgttgccag cgccagaaac
     5101 gccggtttaa ccagcgccga agtgagcgca gtgattaaag ccatgcagtg gcaaatggat
     5161 ttccgcaaac tgaaaaaagg cgatgaattt gcggtgttaa tgtctcgaga aatgcttgat
     5221 ggtaaacgtg agcaaagcca gctgctgggc gtacgtttgc gttcagaagg taaagattat
     5281 tacgctatcc gcgctgaaga tggcaaattc tacgatcgta acggtactgg tctggcgaaa
     5341 ggattcttgc gattcccgac ggcgaaacag ttccgtatct cgtctaactt taacccgcgt
     5401 cgtactaatc cggtgaccgg tcgcgttgca gcacacagag gtgttgattt cgccatgccg
     5461 cagggtacgc cagtgctttc agtgggtgac ggtgaagtgg tggttgccaa acgcagtggc
     5521 gcagcaggtt attatgtggc tattcgtcat ggtcgcagct acaccacgcg ttatatgcac
     5581 ttgcgcaaga ttctggtgaa accgggacag aaggtgaaac gtggcgaccg tatcgcgctt
     5641 tccggtaata ccggacgttc aaccgggccg catctgcact atgaagtatg gataaaccag
     5701 caggccgtaa acccgctgac ggcaaaactg ccgcgtaccg aagggctgac cggctccgat
     5761 cgtcgcgaat tcctggcgca ggccaaagag attgtgccgc agctacggtt tgattaatta
     5821 acatccattc gcagccggta cgcagtcagt atcggctttt tttatttggt gcggggcaag
     5881 ttgcgccgct atactatcac cagattgatt tttgccttat ccgaaactgg aaaagcatgg
     5941 aaacgaaaaa aaataatagc gaatacattc ctgagtttga taaatccttt cgccacccgc
     6001 gctactgggg agcatggctg ggtgtagcag cgatggcggg tatcgcttta acgccgccaa
     6061 agttccgtga tcccattctg gcacggctgg gacgttttgc cggacgactg ggaaaaagct
     6121 cacgccgtcg tgcgttaatc aatctgtcgc tctgctttcc agaacgtagt gaagctgaac
     6181 gcgaagcgat tgttgatgag atgtttgcca ccgcgccgca agcgatggca atgatggctg
     6241 agttggcaat acgcggaccg gagaaaattc agccgcgcgt tgactggcaa gggctggaga
     6301 tcatcgaaga gatgaggcgt aataacgaga aagttatctt tctggtgccg cacggttggg
     6361 ccgtcgatat tcctgccatg ctgatggcct cgcaagggca gaaaatggca gcgatgttcc
     6421 ataatcaggg caacccggtt tttgattatg tctggaacac ggtgcgtcgt cgctttggcg
     6481 gtcgtctgca tgcgagaaat gacggtatta aaccattcat ccagtcggta cgtcaggggt
     6541 actggggata ttatttaccc gatcaggatc atggcccaga gcacagcgaa tttgtggatt
     6601 tctttgccac ctataaagcg acgttgcccg cgattggtcg tttgatgaaa gtgtgccgtg
     6661 cgcgcgttgt tccgctgttt ccgatttatg atggcaagac gcaccgtctg acgattcagg
     6721 tgcgcccacc gatggatgat ctgttagagg cggatgatca tacgattgcg cggcggatga
     6781 atgaagaagt cgagattttt gttggtccgc gaccagaaca atacacctgg atattaaaat
     6841 tgctgaaaac tcgcaaaccg ggcgaaatcc aaccgtataa gcgtaaagat ctttatccta
     6901 tcaaataaaa aaagcctctc aaattattga gaggctgtac tcgataaaaa atctcaatga
     6961 gtgttcccac tattttgagt ttttaggtta gtacgctatt gagttaatcg tagcgtgata
     7021 atttcaaaag gtttgaactc aagattgcaa tattgattgc aataaaaact gtcatctaac
     7081 ttattttctt gaagatcgac atattgaacg tagtgataat tacatactca cgaaagcatg
     7141 gctgaaattt ctaatccatg actctcatat aatcttacga ttataccgtc gccgtcttca
     7201 gctaatttta ttgtttcgat gataacgctg ttagtatcga tagcaaaaaa tgatagtgaa
     7261 gggggtaatt cacccgggtg cggtttgaca gggacgacct gtaagggatg attcattata
     7321 taggcttgat taataacacc acccacacgg taatctccat gatgaggaaa taaggagaat
     7381 atgaattttt gtttaccaat atctgcaaaa ttattaatca ggaaattatc ataattgtct
     7441 gtcttaacaa cttccccagg tttatgctgc gaacgcagca aagttaattc aagcgtctgg
     7501 tctttgacac gatagccata tttgtttttt gctattaaag ctatgccagt ttctcgatca
     7561 gaaaggtcga cccagtgatg tgctggtatt tcatctttgg caaaagcgaa cgaatcatcg
     7621 ctatgcgttg gtctgtcaat tacaccgaac tgaatttgac attgtgcaag agatgtttgt
     7681 actgacaccg ggaattttac tttgagagaa acattaggtt cgtgccaatc aataaacgtt
     7741 tggacatcaa cgcgtggact atcctgtgtc agggtaaagg ctaaacgaag ataagaggaa
     7801 ttgtagtgat aaatcagcgt catcgtagtt ttaggcccgc tgatcacagt tgtcattttt
     7861 tcagcgagta aaacatatga ttccccatca cgataatttg aagcaaaatc ccaggccgcg
     7921 aaaaaacctg catcttcgtg gtatgcacgg atctcactgt gcatattttc agtaatgtat
     7981 tctttaccat atcttttatc atatagcgaa attaaatcac cgctttctga aaaaagaagt
     8041 ttaatgtaat tattttcaat actttgagat tctgccttca gaccatttac gatcttattc
     8101 tttggaacaa tcacagcata accataagag tttactcgtg ctttgagcca gttgttgttt
     8161 atattgatcc attcgttacg agcaaaacag gtggtattaa acaaaatgtg cggatcttta
     8221 taagaagaag tgtcaatttt acttaataaa gtagactgcg catcagaaat aattttctcg
     8281 gtttttgctt ctagtttaag atattccttt tcagtttcat gataaactct tg
//
LOCUS       DAZPJR010000174         8317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPJR010000174 DAZPJR010000000
VERSION     DAZPJR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.178"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            76..738
                     /locus_tag="ACHVB7_003669"
     CDS             76..738
                     /locus_tag="ACHVB7_003669"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR1915551.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAVVVFKKEPRKHEITQTVCCGD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LS"
     gene            1121..2074
                     /locus_tag="ACHVB7_003670"
     CDS             1121..2074
                     /locus_tag="ACHVB7_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1915552.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEEELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            2074..2370
                     /locus_tag="ACHVB7_003671"
     CDS             2074..2370
                     /locus_tag="ACHVB7_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915553.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            2694..2984
                     /locus_tag="ACHVB7_003672"
     CDS             2694..2984
                     /locus_tag="ACHVB7_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915554.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            3205..3420
                     /locus_tag="ACHVB7_003673"
     CDS             3205..3420
                     /locus_tag="ACHVB7_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915555.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            3567..3872
                     /locus_tag="ACHVB7_003674"
     CDS             3567..3872
                     /locus_tag="ACHVB7_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915556.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            4348..5058
                     /locus_tag="ACHVB7_003675"
     CDS             4348..5058
                     /locus_tag="ACHVB7_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1915557.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            5083..5289
                     /locus_tag="ACHVB7_003676"
     CDS             5083..5289
                     /locus_tag="ACHVB7_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915558.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            5388..7568
                     /locus_tag="ACHVB7_003677"
     CDS             5388..7568
                     /locus_tag="ACHVB7_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR1915559.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            7725..8317
                     /locus_tag="ACHVB7_003678"
                     /pseudo
     CDS             7725..8317
                     /locus_tag="ACHVB7_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640578.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
BASE COUNT         2715 a         1496 c         1774 g         2332 t
ORIGIN      
        1 gcctgttaag tatctcttat cttaaaagag agtaaacctt gtcaagagtg attgcaataa
       61 aattttttat ttcttatgtc aattgagcat attttaatcg ggaaacatca tgggggttct
      121 gattactgga gaactccatt gttgttattt aagaacttac accgtgaatt tggttttacg
      181 ctagatggtg ctgcaactga gcatgacacg ctgttaccac gattcaccaa tgatataagc
      241 cgtcaatcat gggttagtga aaaggtcttc tgtaacctgc cgtacagcga tattccgtcg
      301 ttcctcttaa aagcgtctga agcggattta gtagctttcc tgataccgca tagagccaac
      361 accagctact ggcttaggca catttacagc aataaccact gtcatgaaat ccgtattctt
      421 catcgtgcag ttaaatacct tccaccagca ggccataacg ggcttactat tcgttcaccg
      481 tttcctagtg cagtcgttgt ttttaaaaaa gaaccacgta agcacgaaat tacacagacg
      541 gtttgctgtg gtgacacgct attaccgtta accatcatta acagaggagg cttgagagga
      601 agacccacca tttacccacc tgagacgctc gacagcttca taaaactata caggcaagga
      661 aaacccatta agtgcattgc agatgctctc agaatgcctc tgagcacgtc atacaggatt
      721 gctcaaaggt tgagctaaaa ttctcataaa tatggtcatt tacaagatag gtgattcaga
      781 tattaagaag tatccctgtg aacaataaat gaccaatcag taattattct gtgaaataat
      841 tgttgcaatt tataatcaca tctgttatag tttttatatg gggtgataaa agaactcaga
      901 acattaagag gttacaacgt tgggaggcgt cagggtattg acagattttc acggcctctt
      961 attcaatcaa ggcttttcct tttgctgtta atactcgcgt aatcgctcag gcttctgagt
     1021 tcctttatca gtccatcttc taattaattc actccctctg gggttggtca ctatacgtgg
     1081 tcagcccttt ctttgtttta tttttaaaaa ggaaaatata atggaaaaat tccacatgtt
     1141 tgatgaccgt atttatcaga tgactaatgg cgttaaagct gccgaccgta ttatttacac
     1201 tgtcatgttg aatgaacaaa agttttatca cgataagggg caatattata ccccttcata
     1261 tgctcatctt ggtcagtatg ctggaatcac aagaacatcc gtgattgcat gtatgaaaca
     1321 acttgaggca catggtttaa tcaagaaaac taacgttgca agcaatcagt gtgttattaa
     1381 agtttattca cttgatgaag taccggaggt tttaacagag cttacagcta aggaagccag
     1441 aaaggcaaag ctggagtcta aagttactca gtcagtcaag aaagatgacc cactgagtca
     1501 aaattcttta cccactgagt caaaattttt tactcagtca gtcaaaatct ttgacacgta
     1561 taataacagt aataacaaga aagaacttat taataacaat aaagaagatg actttgaaga
     1621 agaatatttt aagaagggag cttcgctctg tgattcacat gaatgtgatt ctcttggtat
     1681 tgaagaggct attaaagaga ctgttaaaac atctgactta gatgaccttg atagtgaagc
     1741 tgctaaagag gtcgttaaaa aggaagataa aatctctgat gtgattgcta gtagtgacca
     1801 taaaaacaca cagtgccccg ccactgatga tgaattaccg gatgttgacc ttgatgatga
     1861 agaagaccct gaagaggaat tgaaacgtga acagcgtaaa gcagaaatta aagaaagatt
     1921 cagaaatgcg aagctaaaga acaaggttag tgacctcgat gactttgatg atagtgacga
     1981 tgattttact tttacaccaa aaaagaaaac tcacaccgag ccaaagaaag aaaaagttaa
     2041 agtcagacgt gtttatggtg gaggaatgtt ttaatgaaaa cttataatgg cgagagaaaa
     2101 taccgtgact atttactcag acgttacagc atttctcgtg aaggttactt gatgaaagat
     2161 actcacgggg aagtttaccg gattcgaccg aagaaagaag ggagggatta ttatttcgtc
     2221 gatgatgtga cagatttaaa gattgacgct ttgaagtttg cggtgctgta tcattacgat
     2281 ttatgggatt cagtccacca gctacgatta aaagacggtg accccggtaa tctgagggcc
     2341 accaatatta tcaagggaaa gtgccggtaa ggccaccagc tatttattta ctgaaatctc
     2401 tatctcaaca cgaagaagtt taagtcgaat tgtaagcttt gtcttttttg taatgactcg
     2461 attcataacg caaaacacca atagcacaat aaataccgca aagcccaacg agctaaaacg
     2521 atgagattca ataattgctg tgaaaaaatc atgcattaat tttccttttg tgaaaggaaa
     2581 cagcacctgt atcagcccct acaaccatag tatcatgtaa atgaggtact gacaatatgt
     2641 taaatagatg ttaagattgt agtatgagta agagatttaa ggagattttt acaatgactc
     2701 acacagttac gcgccttttg ccagaggcat ttgaacgact ggcagcaatc aaagccaaat
     2761 atcccgatat gaagcacaca gcgcttgttg gggagatttt aaaagagtcg gtaaacctta
     2821 gccctttcga taacgttaag ggcgtcgcag agggttctaa ggccatcaga atcacaacgg
     2881 gccaactgga actactcaaa gcacagtccg cagtagcagg ttgctcaatg attcaggtgt
     2941 taagcgctaa ggtccatcaa ctggcggata gattggaggt ttaatgtgtt ataggttcca
     3001 atattccaca cttggccgcc acctaatcag acagaattta gcagatgctg aatttgaaga
     3061 tgtgaccctc aaagcaaaca aacgattaaa accctctcaa atccaccagc ttaaacacga
     3121 attaaagcca ctggtaatac tcgactataa cgaccctgaa cagcttgagc agggaattaa
     3181 gcttatttct gaatggggtg cttgatgaaa actatcacag tacaacttca gactaataaa
     3241 gcatttcgtt attttgaaaa cttgcttgag ttatacgaag gatggggaag tatccacggc
     3301 aaagatgata tctatcttca cttgtcagcc cctaactatt ctctgaagac tccggttaag
     3361 caaagctggt taaaagacta cggtcaccag atggggctat tagtctcaga cttgagctaa
     3421 aaacagcaaa acatatctct caggctttac cagacgcacc agaaacgttt ttaagcccct
     3481 aatcagggta acttagggaa tcgccttaag tggtctaaaa aaggggttaa aacgtcttag
     3541 aatgcgatac aggaaggatt ttaaaaatga attacccaat gcaaattatg gttgaccaag
     3601 aaaccatgaa ttggttgatt gacctgaaca ggtcgggctt taatatctat ttcatgcatt
     3661 gttttgaagc gtcaacgtat attgacaagc atataatcaa cgaaattaaa cttggtgact
     3721 atgttcacaa gctggaagta acagacccat acaataagcg ctttatcatt gaagagtgca
     3781 atcgacttga tttaagccct gagcaattat tgaagctgaa tgtattcttg acctatcaga
     3841 atgaattaaa cccttacagc gaggtgatgt aatgacactc gttattctct ggatgacttt
     3901 agggttattt gttggttgct gggtaggtaa tacggtaaat aagaattgat gttaccaatc
     3961 tgttaaaaag ctgttgacaa ataaccttaa gcatgagatg atggtaatag ggaagagcaa
     4021 ctcttcctga attaaactat taatcattct acatcaatga tagttattct gaaaggtgca
     4081 atgcttttcg ctacatgacc tttcaggtta gtctgagagg tcatctcgaa aagtactctt
     4141 gttcattcgt ttgaatcctt tttgaatcct ttagatgttt taatttcttc accttatgaa
     4201 tggcccttta taagaaatta tctagggcta ttcagctttt aaaaagcata cttggcaaac
     4261 cgccttttaa gtctcttcta agtttagata ggctgtcagg tgtgcttttt aaaaactgaa
     4321 acacttccgc ttagaagagg aaaagcaatg acaaatgata tttacattaa tggcaaagtg
     4381 attgatagct tcagtgactg gtcgccacag actgaacatc cagctatcac tattcagcgt
     4441 aaagagcatg acaagcgtat tgagcttgag caatcgctaa agatgtcacc aaaaacaacc
     4501 atctttgtgt ctcctgagcc tcaagagatg cctgatattt gcaagagtga agcattactt
     4561 gagcttgaaa agaaatatta ccaactgctg aaagaacagc gccttagatt agatgacgct
     4621 tacagcaaag ttaccttgat gcaatcagct attgagccgt cagaatttga gattcaggat
     4681 gaattgtcac agaaaccatt tgtctattac caatacgaag ataatgatgg ttttggtact
     4741 tttcaagaaa acgtcgcagc cgtgattagt agtcttcctg ctggacatcg aattgtgaag
     4801 atggttaagg catcaagagg tgctggtcag tttatttaca tgactaataa gagcgatgaa
     4861 gaactcgctg aaattgctag acagaacatc ttggcatcac gtaacaagca acttgataat
     4921 gttaagttgt atctctcaag agagcttcag gctatgaaag agcttgtgaa agaatatgaa
     4981 gatgctaaga agattgctat gcaggctgat attgagcagc ttacgaagat ttctagcaaa
     5041 tatgcaaagg ctatttaatt aataattaaa aggtggtggt gaatgattga tttaacagaa
     5101 cgctatgagc ttattaaagc aattagtgaa cgattgaaag aacaacccaa caaaccaaaa
     5161 cgaatcaaat cattcacctc ctctaagcaa aagaatagaa tcaggaaaat agctaagtgg
     5221 tgtcttgata aattaccaag tgattgtcag attgttggtg aagatggtaa ttattatctg
     5281 gttaggtaac aagattggtt gggtgaaaat ctacgggaga gattatcgag tgatatcggt
     5341 agtctttccc tttttcgtat atgaacactt aaagaggtga aacatgaatg agcaaattca
     5401 cagttgatag ttttatcgtt gaacttagtt tcagtgaaaa cgttattaag ggcttgcaaa
     5461 gagttgaaaa ggcagctttg cagtcagcac aaagaattga acgcaatttg aatagagcgt
     5521 ttaaggtcga taccaagcaa cttgacagca atctgacaag ttctttaggg cagcttgaaa
     5581 ggaagtttaa taagaccttt gacaagattg agcagagagc aagaagcaca aaagcatttc
     5641 agtttgccac cagatttaat gacactgtta atcctccaaa acaatcaagg caaccacgaa
     5701 ttagcggaaa cagagcaatc acagcagcgc atagcgtgaa tatgtctaag cttggtgata
     5761 ttaatccgct gatggctaaa atgtttaaag cacagtatta cagcttaagc ggtaaggctg
     5821 gtaatattgg aagtgaaaag tttaacgctg aacttgcaaa attaaatcaa tctcttcgtg
     5881 aaacgttgaa taaactcagg tcacagacaa agaccgccag cattaacaat acaagcgacg
     5941 catttaataa tcttgcaagc aatgcgatta aattatctgg tgcattttac tctgtgatgg
     6001 gtgctcttaa cgcttacaag gccattatga atgcaggtct taaacgagat tccgcacaga
     6061 gagccgctaa gtttgtactt ggtgataaag catctgaagc tgaggtattt attcgaaatt
     6121 tagcagacaa gacaggtctt aatatctctg aagggctatc aagctacgct aagtttgcag
     6181 caggtgctca gggttctatg tcacaggaac agacacagga gctatttggt aatgcaacgg
     6241 ctatgagtcg cttaatgggg ctttctaacg atgagctaaa cggcattttg agagcatttg
     6301 agcagatggc gagcaagggc aagattcagg ctgaggaatt acgtgggcag ttaggtgacc
     6361 gtatggctgg tgcatttaag ctattcgctg aagcattggg tatgactgct acagagcttg
     6421 atgcagccat gaaaaacggg aaggtacttt ccgctgatac tctccctaaa gtcgctaaac
     6481 agatgggctt gatgattgat aaagcgggtg gctgggctga agtcactaag agcactcaga
     6541 cagctttagg taagttagca aacaattggg atgatacgat ggtgaaaatc ttctcaggtt
     6601 cacaggatga gctaaacggc ttcttatcca gcctgagtaa cctgcttagt gaaatgggga
     6661 tgagttcttc tatcgctggt gatgctatcg gtggacttat cgacatgctt aaagctggtg
     6721 tagatgatat tcgcgtattc aataacacca ttgaaggctg gattttacag actaagaagt
     6781 tctattacag ccttgatgac actaaacgaa aactacttga tgaagtcggt gatggtttta
     6841 tcaacttcgt caaaggtctt gctatagcgt tatctgcaaa gacgctattc agtgctacta
     6901 caggggttat gaacctgaca agagcaatca caacgttagg tacaagagct aatcaggtgg
     6961 ctggtcaggt agctactggt aaaggtggtg gaaaactgaa aggtgtagct ggtggtgtag
     7021 gtagtgctct tgcaattggt tatgctgatg acggttatga aacagcgtta gcattagctt
     7081 caatgattcc acaaatcaga ggcgtcactc ttggtttata tgcattgaag aaagcactgg
     7141 attttatgaa tcaggaagta gtcaaaaacg ctaatatgca tccgtctggg gtaggtgtgg
     7201 gtagtgactt taatcccgtg tattctggtg accctaataa accgaatatg attaacgaag
     7261 gatggagtag aatttttagt tcaatgggtg aattgtttaa caatgccaca atgaatattg
     7321 caagattcaa tcatccagag ttaaacaaca ttaagcaaga ttcagcatta acgagtgcag
     7381 atattcaggg tctgagggat gaaattagtg ctttgagtaa gcgtattcag gagccagtga
     7441 aagtttcact tggtggtcaa gttgtcgttc gaccagatga accatctttc tttactttca
     7501 atcaatcagc taatgaccag ttcacgcagg atatgctctt aagttcaagc tatccagagg
     7561 aagaatgatt taacggtgtt ctacgtgaat aaagcaaagt taatacccac accaaaaatt
     7621 atttttcacg tagctcacgc ttaacagaga aaatacatat atatcaaagg cttggttaaa
     7681 aatgattaaa atacactaag cctttgaaat caaaggagaa tttaatgatt tacactaatc
     7741 agcttcgtta cagtggcaaa gatggcatct atttccactt aagagacaat gtagatgcct
     7801 tcttaacctt atcagcaact gaaaacatgg aatttgatag ccctatgcag gtgactacac
     7861 agaacatgca attatgaagg aagcttatta ttaactcgtc agggtcaatt agtagaaaac
     7921 ttcattgaca ctctggaaaa ctggcgtgac cagaagcaga ttatttctgt tatctgtaaa
     7981 gatggaatta aaattgacga ttccattatt accaacttta aagcctctaa agatgttact
     8041 atctctaacg gtcttcgtat ccagctaact tttcaggaaa ttaactttaa agcaatcgta
     8101 ggtcaaactg atatttcagc agccactggc aaaacggcta ccacaaatga cggtggtgtg
     8161 accagtaaga agaatgcagg taacgtgacc actgaacgca ataacgcgcc tatgctatcg
     8221 tgcaaagagt taatgaatat tagcgatgta tccggttacg ctccagctat cagaaatgat
     8281 attctcgatg ctcagattaa atgtaataag gcttgga
//
LOCUS       DAZPJR010000175         8273 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPJR010000175 DAZPJR010000000
VERSION     DAZPJR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.179"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            52..936
                     /locus_tag="ACHVB7_003679"
     CDS             52..936
                     /locus_tag="ACHVB7_003679"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR1915560.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHVB7_003680"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHVB7_003680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR1915561.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHVB7_003681"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHVB7_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR1915562.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHVB7_003682"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHVB7_003682"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR1915563.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="ACHVB7_003683"
     CDS             5160..6443
                     /locus_tag="ACHVB7_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR1915564.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="ACHVB7_003684"
     CDS             6590..8065
                     /locus_tag="ACHVB7_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR1915565.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2192 a         1980 c         1915 g         2186 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacgtta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgc tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgacctgg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcagaaac tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgagacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actgaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc cgg
//
LOCUS       DAZPJR010000176         8217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPJR010000176 DAZPJR010000000
VERSION     DAZPJR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.180"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..103
                     /gene="mgtT"
                     /locus_tag="ACHVB7_003685"
     CDS             <1..103
                     /gene="mgtT"
                     /locus_tag="ACHVB7_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR1915566.1"
                     /translation="NGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHVB7_003686"
     CDS             complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHVB7_003686"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR1915567.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHVB7_003687"
     CDS             complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHVB7_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR1915568.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2031..2549
                     /locus_tag="ACHVB7_003688"
     CDS             2031..2549
                     /locus_tag="ACHVB7_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR1915569.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="ACHVB7_003689"
     CDS             complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="ACHVB7_003689"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR1915570.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4776..5522
                     /gene="ydfG"
                     /locus_tag="ACHVB7_003690"
     CDS             4776..5522
                     /gene="ydfG"
                     /locus_tag="ACHVB7_003690"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR1915571.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5611..6297
                     /gene="rspR"
                     /locus_tag="ACHVB7_003691"
                     /gene_synonym="ydfH"
     CDS             5611..6297
                     /gene="rspR"
                     /locus_tag="ACHVB7_003691"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR1915572.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6474..6677
                     /gene="ydfZ"
                     /locus_tag="ACHVB7_003692"
     CDS             6474..6677
                     /gene="ydfZ"
                     /locus_tag="ACHVB7_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR1915573.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6713..8173)
                     /locus_tag="ACHVB7_003693"
     CDS             complement(6713..8173)
                     /locus_tag="ACHVB7_003693"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1915574.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2173 a         2055 c         1871 g         2118 t
ORIGIN      
        1 gaacggagat aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga
       61 tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga
      121 tttgtgtttt gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac
      181 agtagcatca gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt
      241 gttcgtcaat aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt
      301 ctgctcttcc aatgaccaca tccagaggct cttcaggaaa tgcgcgactc acacctgctg
      361 tcacggtaat gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc
      421 aaattctgac acctgcacga catgcttctt catcattagc cgctttaaca ataatgataa
      481 attcttcgcc cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag
      541 ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt
      601 taaatcggtc aatatccaac aacattaaat aaagattcag aggatcagtg ttgcgtaact
      661 gatgatcaaa ggattcatca agaacccgac gccccggcaa tcccgtcaaa acatccatat
      721 tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca
      781 acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca
      841 ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag
      901 gtaattcatc gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc
      961 cataagaatg gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa
     1021 acatactcac cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg
     1081 catcaatttc cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg
     1141 ctattctaac gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg
     1201 agtcatctct gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa
     1261 ttctgtaaat cacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata
     1321 ccctaatggc ccagtagttc aggccatcag gctaatttat ttttatttct gcaaatgagt
     1381 gacccgaacg acggccggcg cgcttttctt atccagactg ccgctaatgt tgatcatctg
     1441 gtccggctga acttctcgtc catcaaagac ggccgcagga ataacaacat taatttcacc
     1501 gctcttatcg cgaaaaacgt aacggtcctc tcctttgtga gaaatcaaat taccgcgtag
     1561 tgaaaccgaa gcgccatcgt gcatgttttt tgcgaaatca acggtcattt tttttgcatc
     1621 atcggttccg cgatagccat cttctattgc atgaggcggc ggtggcgctg catcctgttt
     1681 taaaccgccc tggtcatctg ccaacgcata aggcatgaca agaaaacttg ctaatacaat
     1741 ggactgaaat ttcatactaa ctccttaatt gcgtttggtt tgacttatta agtctggttg
     1801 ctatttttat aattgccaaa taagaatact gccaattgtt ataaggcatt taaaatcagc
     1861 ccactagctg tcaaatattc agagaatttg actcactaaa gttaagaaga ttgaagagtc
     1921 ttaaacatgt tttcagaata atcggattta tatgtttgaa aattattata ttggacgagc
     1981 atacagaaaa agcaaatcac ctttacatat aatagcgtgg acaaaaaaca gtgaacatta
     2041 ataaagataa aattgtacaa ctcgcagata ccgacactat tgaaaacctg acatccgcgt
     2101 tgagtcaaag acttatcgcg gatcaattac gcttaactac cgccgaatca tgcaccggcg
     2161 gtaagttggc tagcgccctg tgtgcagctg aagatacacc caaattttac ggtgcaggct
     2221 ttgttacttt caccgatcag gcaaagatga aaatcctcag cgtaagccag cagtctcttg
     2281 aacgatattc tgcggtgagt gagaaagtgg cagcagaaat ggcaacaggt gccatagagc
     2341 gtgcggatgc tgatgtcagt attgccatta ccggctacgg cggaccggag ggcggtgaag
     2401 atggtacgcc agcgggtacc gtctggttcg cgtggcatat taaaggccag aactacactg
     2461 cggttatgca ttttgctggc gactgcgaaa cggtattagc tttagcggtg aggtttgccc
     2521 tcgcccagct gctgcaatta ctgctataac caggctggcc tggcgatatc cccaggccag
     2581 ccattggtgg tgtttatatg ttcaagccac gatgttgcag catcggcata atctgaggag
     2641 ccttaccgcg ccattgtcga tgcaggcgtt ccagatcttc gctgttacct ctggaaagga
     2701 tcgcctcgcg aaaacgctgc ccattttcac gcgttaatcc gccctgctca acaaaccact
     2761 ggtaaccatc atcggccaac atttgcgtcc acagataagc gtaataacct gcagcatatc
     2821 cgccaccaaa aatatgggcg aaataactgc tgcgatagcg tggcggtata gcaggaagat
     2881 ccatattttc cgccaccagc gcccgcaatt caaaatcatc gacatcctgc attgcttcgt
     2941 tttcttccag gcaatgccag cgcatatcga gaagtgcggc gctaagcagt tcgctcatct
     3001 catacccttt gttgaacagg ctggcattac gcattttctg ttgcagttcg tcaggcattg
     3061 ctgccccgct ctgataatgc cgggcgtagc gagcgaatac ctgcggatgc gttgcccagt
     3121 gttcgttgat ttgcgacgga aattcgacaa aatcacgcgg cgtgttggtg ccggaaagcg
     3181 tggcataacg ctggcgggca aaaaggccgt gcagcgtatg accaaattca tggaataagg
     3241 ttattacatc atcccagagt aacaacgcag gctcaccggc agcgggtttc tgataattgc
     3301 agacgttata aattaccgga tgcgtttcat taagcgttga ttgctcaaca aaattgccca
     3361 tccatgcacc gccgcttttt gaatcacggg cgaagaaatc accgtaaaat aacgccagcc
     3421 ccacgccatt atgatcaaaa atttcccaca cccgaacgtc aggatggtag acaggaatat
     3481 caaaacgttc gacaaactta ataccgaaga gctgattcgc ggtccagaat acaccttcat
     3541 tcaacaccgt gtttaattca aaatatggct tgagctgcgc ttcatcaaga tcaaatttct
     3601 cccgccgtac ctgttccgca taaaatgccc agtcccacgg ctgcgcgcta aacccgccct
     3661 gctgcttatc gataaccgcc tgtatggagg ctaattcatc gctcgcacgt tgacgcgccg
     3721 ctggaacaat ttcccgcata aagttgagtg ctgcttctgg cgtttttgcc atctgatcgg
     3781 cgattttcca tgcggcataa tgaggaaaac caagcagttt cgcctgctgt gcgcgaatct
     3841 ctaccagacg ttgaatgata gcgcgggtat cattgccatc atttttttcc gctcgcgtcc
     3901 agcccgcaat aaacagtttt tcacgcgtcg cacgatcgcg catttcggca agcgccggtt
     3961 gttgggtggt attcagcagc ggaatcagcc atttgttatc cagacctttc tcgcgagccg
     4021 cctctgccgc cagcgcaatc tcttgctcac tcattcctgc cagctgcgcg atatcgttca
     4081 caaccagacc gccggattta tttgctgcca gtaatcgctg gttaaactgg ctggtcaggg
     4141 tcgcagcttc tgtattcagt acttttaatt ttgctttatc agcttgcgca agtttggctc
     4201 cggcaaggac aaaacgttga tgaatcacct ccaccaggcg gatggattca ctatcaagcc
     4261 ccagggattc acggcgctgc cagacagcat ctacccgcgc gaataattca ccgttcagat
     4321 agatatcatt agccagttcc gccagttcag cggaaaactg ttcatcaaga cgctgtaatt
     4381 catcattggt atgcgccgca gtcatcgcaa aaaagacgct ggtaacgcgg gtaagtaatt
     4441 ctccgctttg ttccagtgcc agaatagtat tgttgaaatc aggcgtttgc gggttaagcg
     4501 cgatggcagc aatttctgcc cgcttttgct gcattccctc atcgaatgcc gggcgatagt
     4561 gatgattggc aatttgatca aaatggggag ccagatacgg tagtgtgctt tgcacaagga
     4621 aaggattcat tgttgtcatt ttcttctcct gaacgcgaga tgttccatag cgtaggctta
     4681 ctgataacgg agtgcaatct tgcaaaccag tattacccgc tcttaagcat cacgtgctat
     4741 gttattgaca cacaaaagcg ttgaggaaca gtgagatgat cgttttagta actggagcaa
     4801 cggcaggttt tggtgaatgc attactcgtc gttttattca acaagggcat aaagttatcg
     4861 ccactggccg tcgccaggag cggttgcagg agttaaaaga cgaactggga gataatctgt
     4921 atatcgccca actggacgtt cgcaaccgcg ccgctattga agagatgctg gcatcgcttc
     4981 ctgccgagtg gtgcaatatt gatatcctgg taaataatgc cggcctggcg ttgggcatgg
     5041 agcctgcgca taaagccagc gttgaagact gggaaacgat gattgatacc aacaacaaag
     5101 gcctggtata tatgacgcgc gccgtcttac cgggtatggt tgaacgtaat catggtcata
     5161 ttattaacat tggctcaacg gcaggtagct ggccgtatgc cggtggtaac gtttacggtg
     5221 cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg tacggacctg catggtacgg
     5281 cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg caccgagttt tccaatgtcc
     5341 gctttaaagg cgatgacggt aaagcggaaa aaacctatca aaataccgtt gcattgacgc
     5401 cagaagatgt cagcgaagcc gtctggtggg tgtcaacgct gcctgctcac gtcaatatca
     5461 ataccctgga aatgatgccg gttacccaaa gctatgccgg actgaatgtc caccgtcagt
     5521 aatttttata cccggcgtaa ctgccgggtt attgcttgtc acaaaaaagt ggtagactca
     5581 tgcagttaac tcactcacaa gcaagaacga atgaccgtcg aaacgcaact taatcccaca
     5641 cagcctgtca atcagcaaat ttatcgtatt cttcgtcgcg acattgtcca ttgcctgatt
     5701 gcaccaggca caccgttgtc ggaaaaagaa gtttctgttc gtttcaatgt gtcacgccag
     5761 ccggttcgtg aagcctttat taaactggcg gaaaacggcc tgattcaaat tcgtccgcag
     5821 cgtggcagct acgtcaacaa aatttccatg gcccaggtgc gcaatggcag ttttatccgt
     5881 caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca tgattaccga aagccagtgc
     5941 taccaactgg aacaaaatct tcaccagcaa cgcattgcca ttgagcgcaa gcaactggat
     6001 gatttttttg aacttgatga caacttccat caactcctga cgcagattgc cgactgtcaa
     6061 ctggcgtggg ataccattga gaacctgaaa gcgaccgttg atcgcgtgcg ctatatgagt
     6121 ttcgaccacg tttctccacc agaaatgctg ttacgccagc atcttgatat tttctctgcc
     6181 ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga cgcaacattt gcaggaaatc
     6241 agcgaatccg tgcgccagat ccgccaggaa aatagcgact ggtttagcga agagtaattc
     6301 atttcctctc atcccatccg gggtgagagt cttttccccc gccttatggc tcatgcatgt
     6361 atcaaaaaag atgtgagctt gatcaaaaac aaaaaatatt tcactcgaca ggagtattta
     6421 tattgcgccc gttacgtggg cttcgactgt aaatcagaaa ggagaaaata cctatgacga
     6481 cctacgatcg taaccgtaac gcaatcacca ctggcagccg tgttatggtt agcggcaccg
     6541 gtcacactgg caagatcctg tcgattgata ctgaaggtct gaccgctgag caaatccgcc
     6601 gcggaaaaac cgtagttgtt gaaggttgtg aagagaaact ggcacctctg gacctgattc
     6661 gtctcggcat gaactaagcg tgtgaatgcc gccgatggcg gcattgcttt ttttacttca
     6721 cggaatattt cgccacggtc gctttcgcgc catgcgctaa taaagacaag tacgcttccg
     6781 tcacttttgc agtaaacaaa ctattgtctg gcaaatcatc accaaagatc gccttaatcg
     6841 ccagcaatga ctggacgcgc gctttccctt cagcactact ttgtacagcc ttctgaataa
     6901 caggtaacag tgggtcactg atttcaatcg gatttccctg ttcatcaaca ccaccgacat
     6961 aacgcatcca acccgcgacg cccagtgcca gcagatcgaa cttgctgtca tgcgccagat
     7021 gccagcgaac agaatccaac atccgctgtg gcaatttctg gctgccatcc atcgcaatct
     7081 gccaggttcg atgacgtaac gccgggttgc tatagcgttc aattaatcgg ttagcgtaat
     7141 cttgcaaatc aacgccctgc actttcagcg tcggcgcttg ttcctgcaac atcaagccat
     7201 acgccgcata acgataatgt tcatcttcca tgcagtcatt aatgtgctga tatccagcaa
     7261 gataacccaa atacgccagg aatgaatgac tgccgttgag catgcgcaac ttcatctctt
     7321 cataaggcag cacatcacta accagttcgg ctcccgcttt ttcccattct ggacgtccgg
     7381 caacaaagtt atcttctatt acccactggc ggaaaggttc acaggcaacg cccgcaggat
     7441 cgcccacacc ggtaagttgt tcgattttcg ccagcgtatc ctctgtcact gcgggcacaa
     7501 tacggtccac cattgttgat gggaaagtca cgttatcttc gatccattgt gccagtttta
     7561 catcaacagc tttcgcgtat gaagtgacaa cgtcacgcat aacatgaccg ttttctggca
     7621 tgttgtcaca tgacatgacg gtaaatgcgg gaagtcctgc cgctttacgg cgggccagcg
     7681 cctcaacaat cacccctgtt gctgttttcg gctggtgggg attttgcacg tcggcagcga
     7741 ccatcgggtg atcgagcatt aactgtccgg tcgccggaga gtggaaatac cctttttcgg
     7801 tgattgtcag agagacaatc gcgatttgcg gttcacacat cgcagccaac acggtttcta
     7861 agccatccat ctgtacgtgc aaggctttct taacgacgcc aacgacgcga gccgtccacg
     7921 catcggccga catttccgca acggtataaa gattatcttg ctgttgtaaa tcggcaattt
     7981 gctgttcgcc gccgattaag ttgacctcat aatatcccca gtcactgaaa tgttctgtag
     8041 caagaatatc ggcatacacg ccctgatgcg cacggtgaaa tgcaccaaag cctaaatgaa
     8101 taattcttgg ggccaggtta ttacgatcat aaacagggag tgtcgctttt gctgataaca
     8161 aattatttcc cataacaatt ccttaaatat aaatatggca aactatatgt tttgtta
//
LOCUS       DAZPJR010000177         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPJR010000177 DAZPJR010000000
VERSION     DAZPJR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.181"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHVB7_003694"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHVB7_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR1915575.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHVB7_003695"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHVB7_003695"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR1915576.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHVB7_003696"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHVB7_003696"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR1915577.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHVB7_003697"
     CDS             6679..>8182
                     /locus_tag="ACHVB7_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR1915578.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1978 a         2029 c         2322 g         1853 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact tggcggttta tggcacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZPJR010000178         8157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPJR010000178 DAZPJR010000000
VERSION     DAZPJR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.182"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..449)
                     /locus_tag="ACHVB7_003698"
     CDS             complement(<1..449)
                     /locus_tag="ACHVB7_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1915579.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="ACHVB7_003699"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="ACHVB7_003699"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR1915580.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="ACHVB7_003700"
     CDS             complement(1788..2507)
                     /locus_tag="ACHVB7_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR1915581.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="ACHVB7_003701"
     CDS             complement(2691..3017)
                     /locus_tag="ACHVB7_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR1915582.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="ACHVB7_003702"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="ACHVB7_003702"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR1915583.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="ACHVB7_003703"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="ACHVB7_003703"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR1915584.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="ACHVB7_003704"
     CDS             4977..5252
                     /locus_tag="ACHVB7_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR1915585.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="ACHVB7_003705"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="ACHVB7_003705"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR1915586.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="ACHVB7_003706"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="ACHVB7_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR1915587.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8157)
                     /locus_tag="ACHVB7_003707"
     CDS             complement(7903..>8157)
                     /locus_tag="ACHVB7_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1915588.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1923 a         2159 c         1987 g         2088 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaatta
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggtcaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcgaat
//
LOCUS       DAZPJR010000179         8105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPJR010000179 DAZPJR010000000
VERSION     DAZPJR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.183"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(102..797)
                     /locus_tag="ACHVB7_003708"
     CDS             complement(102..797)
                     /locus_tag="ACHVB7_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR1915589.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(790..2217)
                     /locus_tag="ACHVB7_003709"
     CDS             complement(790..2217)
                     /locus_tag="ACHVB7_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR1915590.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2228..2947)
                     /locus_tag="ACHVB7_003710"
     CDS             complement(2228..2947)
                     /locus_tag="ACHVB7_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR1915591.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3475..4329)
                     /gene="rclR"
                     /locus_tag="ACHVB7_003711"
     CDS             complement(3475..4329)
                     /gene="rclR"
                     /locus_tag="ACHVB7_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR1915592.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4555..5880
                     /gene="rclA"
                     /locus_tag="ACHVB7_003712"
     CDS             4555..5880
                     /gene="rclA"
                     /locus_tag="ACHVB7_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR1915593.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5989..6225
                     /gene="rclB"
                     /locus_tag="ACHVB7_003713"
     CDS             5989..6225
                     /gene="rclB"
                     /locus_tag="ACHVB7_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR1915594.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6237..6830
                     /gene="rclC"
                     /locus_tag="ACHVB7_003714"
     CDS             6237..6830
                     /gene="rclC"
                     /locus_tag="ACHVB7_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR1915595.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6990..7859)
                     /locus_tag="ACHVB7_003715"
     CDS             complement(6990..7859)
                     /locus_tag="ACHVB7_003715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR1915596.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2193 a         1983 c         1732 g         2197 t
ORIGIN      
        1 cagcataacc atgacggaac ggggttaagt caggttgtcc ttaacgctat tttcaacctc
       61 gtatgccttc ttcaggttta tgttcagatc tcatatctct ctcagcaatc ctcaataatc
      121 agatacaccg ctttcaccgg accatgaacc ccgacgactt tgataagctc aatatccgcc
      181 gttgagctgg gaccactgat gatgttaata catgacggca ttcgttcacc ggcctgcgct
      241 ttctgatgca atttttctgc gagttgcgct acacgcggca ggattgtgct tttacgcagg
      301 ataaaaagag aagattccgg gagcaggctc aatgaacgcc cgcgctcgac ggcggaaaaa
      361 agaaccacgc ctcccgattc ggttaaacca tattcagcat acacgacacc cactttagcc
      421 tgctctgcct gcgagatatt ctcggcacct ttcgccggat cccaaacaac ggcattgcat
      481 tcctgctgca aacgttcact aatccccaat tcctccagcc tcgtgtcacc gctaatcacg
      541 accgactgat ctcccagctc tttacacaga cgtattgcag cttctgccgc cttcgcctcg
      601 ctggtcagct cacagcgcgt caacataaca tcgctggcaa actgaataaa cgcgtcacag
      661 cgctgctgtt ggttaagttg ggtaagccgc tcgttagcat agttgttaag cggcgcatct
      721 tctgcttgcg gttcaagtcg cagcgggcga cccagtgcct gagcaacgtt attcaaaaat
      781 tcgctccgat tatccattct ttttctcctg cgcctgatgt ttcttaaacc aactacggaa
      841 actctctccg tcagcttcag gaagatcgcg tgcttccatc cagtcgctaa tcgcgccaaa
      901 tttgagtggt gttttgccgc cattgataaa ccagcttgcc gcatgagcgc cggccatcat
      961 cccgactttc cacaatcctg gatgactatt ggcataagcg aacattttta tcgcccgttg
     1021 ctctgctttt gcggtgatcc ctttttcagc catcacccga cgatgacgca aaatcagttt
     1081 tgacagcgga atacgcaccg gacacacgct gtcacaagca gtgcataaag agcaggcgta
     1141 gggtaaatct ttaaaatctt tatagccgcc aagtagcgga gaaatcaccg caccaattgg
     1201 ccctggataa atagagccat atccatgacc gccaatatgg cgatatgccg gacaagtatt
     1261 catacaagcc ccgcagcgaa tacagcgcag cacatcccga aattcagagg ccagcacctc
     1321 agaacgcccg ttatcgacaa taaccagatg aaactcttca ggaccatcaa cgtgccccgc
     1381 ttcgcgcggt cctgtcagcc aggtgttgta tcccgtcaaa cgtgcaccaa cggcactgcg
     1441 cgccagcatg gtgatcaata catctacctc ggcaaacgtg ggggcaatac gctccattcc
     1501 catcacagca atatgcgttt taggcagcgt ggtacacatt cgcgcattac cttcattggt
     1561 caccaggcat accgaacctg tctctgccac cgcgaaatta cagccggtaa tacctatttc
     1621 agcactgagg aaatcttcgc ggattttttg ccggatgaat aaggtcatcg cttcaggcgt
     1681 ttccggcccc tcatagccca gacgttcgtg tagcactcga cggatctgat ggcgatcttt
     1741 atgaattgcc gggaccacaa catgagatgg cggatcttga tccagctgga gaatatattc
     1801 acccagatcg gtttcaatca cctgaatgcc agcatcctgc aacacatgat tgacaccaat
     1861 ctcttcggtc accatcgatt tagatttcac caccttccgg gcatttttgc gttgggcaac
     1921 ctgtaaaatg tagcgggtag cgtcttcttt ggtttttgca aaccagacgt gaccgccgtt
     1981 ttgcgtcact ttttctgaga gctggtacag ataagcgtcg agattactca gaacatgatc
     2041 acgtatctgg gcggcccgat cgcgccactc ctcccagtgc cccaattcat cgaccatttt
     2101 ttgccgattt gccccaatac gctgctgcgc gtttgccacc gctttgcgca tgatcggatc
     2161 ttcaatttgc tgacggatgc gtgtcttaaa atctgtatta ctggttttga tcgacatctt
     2221 tatatcctca gcggctcatc aacacttcag caatatgcat cactttgact ttctgccctt
     2281 cccgttgtaa tcgcccactg atgttcagca ggcaactcac gtcagcacca attaaatact
     2341 cagggcggac ttccatcagg tgcacaacct tttctttcac catctcgccg gatatttcgg
     2401 ccattttgac cgagaacgtg ccgccaaatc cgcagcaggt atcctgttca gcaaaggtca
     2461 acagctccag tccacgcaca tttttcagca gcgtaagtgg ctcgtccttc actcccagct
     2521 tacgggccag gctacaagat gggtgataca cagctctccc ttgcaaactg gcacctacat
     2581 cgactacccc taatttatta acaataaaag aggtgagatc ctgcatacgc gcggcaacct
     2641 tttcggcacg tgatgcccat tcaggttcat ccgccagata cgtcgggtaa ctttttacgg
     2701 cataggtgca agagccagcc ggtgaaataa tgggatcgtc gttatcctcc agtgcggcga
     2761 tcagattttt catccctgga atcgcttctt tgatataacc gctattgatc gcaggctgac
     2821 cgcagcatcc ctgtttctcc gggaaattta cgcgacagcc gagtttttcc agtagcagca
     2881 cggagtctcg tgccattctt gatttcaggg cgtcaccaat acaggtgaca aagaaattga
     2941 cattcacaac taatactcca ttacttcatg cccatttatg cccgatggtt cccctccttc
     3001 cgggggatca atcatcaata aatatggcgt tcttttttaa ttggctagca acataccacc
     3061 cctcatttgt gtgacaaatg ataatctttc atccaaactt caaatgcaat aactaatttt
     3121 atttctttcg gcatcgctca cattttatgg ggaaatcgac gttcattttt ggcataagaa
     3181 atccatttaa aaaggctaca tcccttatac ctattcagag ctacttattc aggggttatt
     3241 gagatataca ctgatggtgt acaaaaaaat caccgacaat aatcacgtat caagtaatag
     3301 tcggggtttt atttaattga agcaatttag tttgcatttt tatttaaaaa ctgagctaga
     3361 gtccatcaga aaaaaatata taaagcccaa accaccgttg gaaatagaaa tagttgaaag
     3421 gctcacaact cccggaatgc ggattatctt tgccctgatt tctggcggtt ttattcaggg
     3481 tgcaagctgt ctgacccttt cccgatattc tcccggagta caaccaaact cgcggacaaa
     3541 cgccttgtga aaagatgatt cactggcata gcctactgac tcagcgatca ccacaacagg
     3601 gagcatttcc cgggaaaaca tctgggccgc tatttgtaga cgcaactttg ttaatacagc
     3661 cagcggcgtg gttccggaaa catcacggaa aagctgggca aaacttgccc gagacatgtg
     3721 ggcgatgctg gccagcgatt cgacggtcca ggcgtgtcct ggcatttcca gcatttgctg
     3781 tattaccgca ccaagacgtg gatgcagaag caaactgaga atgtttttct ctgtattaac
     3841 ctgtgcaatc cactcacgca ccgcgagggt aaagaatgta gcgcagatct ggctacacag
     3901 tgcatccact cccggcatcg ccgatctgga ttcctgttgt aaaaacggga tcgcctccct
     3961 cagccagtta tattccacgc tgtggttaac cggtgctaaa aacagcgttt ccggcaaaga
     4021 cgttaaaaaa taacgcgccg aatgttgcaa ccgaagagtg ccgcagacaa tacaggtcga
     4081 ttcattatcg acatgactca ggcgatgagc tgaattttgt ggtagcagga ccacatttcc
     4141 cgggcgtaat gtaaaaatct ctcccgtcgg catttccagc ttcgccgctc cttgcgttaa
     4201 cgcatgccaa cgaataaccg ataattcccc ggcaccatgt tgaagctgcc agtcacttcc
     4261 taacacgcaa ttcttatcga tcgttccttg tggagcgtta agcatcaaca aacggctaag
     4321 ggcatccata tcagactcct gagtggtttt tgattacaaa caccaacaat cttagtaaat
     4381 aactaatcta ataccatcaa atggttaatc atgatttttg ttttgtgatt ctgattaaga
     4441 aaaagaaaat accagacgat aagacaaaat atcaagcgtt gtgaagaaat ggtatttgct
     4501 cttttgcgtc tatagtcatg atgtcaaatg aacgtgtttc gacaggaaat catcatgaat
     4561 aaatatcagg cagttattat tggttttggc aaggctggaa aaacattagc cgtcacgctg
     4621 gcaaaagcag gttggcgagt ggctctcatc gaacaatcaa atgcaatgta tggcgggacc
     4681 tgtattaata tcggctgcat cccaaccaaa acattggttc atgacgcaca gcagcacaca
     4741 gattttgtcc gtgccataca gcgtaaaaat gaagtggtta attttttacg taataagaat
     4801 tttcataatc ttgcggatat gcccaatatc gacgtgatcg acggccaggc ggagtttatc
     4861 aataatcata gcctgcgtgt tcatcggcct gagggaaatc tggaaattca tggcgagaaa
     4921 atttttatta ataccggtgc acaaaccgtg gttccgccaa ttcctggaat taccaccacg
     4981 cctggtgtat atgacagcac cggattactt aatctaaaag aattgcctgg gcatttaggt
     5041 attttgggcg gcggatatat tggcgttgag ttcgcctcta tgttcgctaa ttttggcagc
     5101 aaggtaacca ttttagaagc agcttcgctg tttttgcctc gggaagatcg ggatattgct
     5161 gataatatcg cgacgatttt acgcgatcag ggcgtcgata ttatcctcaa tgcccatgtg
     5221 gagcgaatca gtcaccatga aaatcaagtg caagtgcata gcgagcacgc ccaactggcg
     5281 gtggatgcac tgttaatagc ttccggtcgt caaccggcta ccgcttcgtt acatccagaa
     5341 aatgccggta tcgcagtaaa cgagcgcggg gcaattgtcg ttgacaagca attacatacc
     5401 accgcagaca atatttgggc gatgggagat gttaccggcg ggctgcaatt tacttatatt
     5461 tcactggatg attaccgcat tgtgcgtgat gagttgctgg gagaaggcag acgtagtact
     5521 gatgatcgga aaaatgtgcc ttattccgta ttcatgacac cgccactgtc cagggttggt
     5581 atgacagaag aacaggccag agagagtggt gctgatattc aggtggtgac attgcctgta
     5641 gctgcaattc cgcgtgccag agtgatgaat gatactcgtg gggtattaaa agcgattgtt
     5701 gataataaaa cccaacgtat attaggggca tcactgctgt gtgttgactc ccacgagatg
     5761 atcaatatag tgaaaatggt gatggatgcc gggctgcctt atagcatatt acgcgatcag
     5821 atatttactc atccgtcgat gagcgaatca ctcaatgatc tattttcatt agtcaaataa
     5881 actcaaaatc agacgccaga acaaatattc tggcgtctca gagaaaagaa tcttattaat
     5941 tctctgttac tctataactt cttaatcact tcattgatgg tattttatat gtttaaaaaa
     6001 tctgttttat ttgcaacact attatctggc gttatggaat tttccaccaa tgcagatgat
     6061 aaaataattc tgaaacatat cagcgtctcg tcagtatcag catcaccgac agttctggag
     6121 gataccattg ctgatatagc cagaaaatat aatgcttcat cctggaaagt cacatcgatg
     6181 cgaattgata ataattcaac cgcaacagca gtattgtata aataaggatg ttcacaatgg
     6241 aaaaatacct gcacctgtta agtcggggag ataaaattgg cctgtcattg attcgtctga
     6301 gtattgcgat tgtttttatg tggattgggt tattaaagtt tgtcccttac gaggcagaca
     6361 gcattacacc attcgtcgca aacagtccac taatgtcgtt cttttatgaa cacccggaag
     6421 actataaaca gtatctgact cacgaaggcg aatacaaacc agaagcaagg gcatggcaat
     6481 cggccaataa tacctatggt ttttccaacg gtcttggcgt cgtggaggtg attattgctc
     6541 tgctggtttt ggctaatcct gtcaatcgct ggttaggttt attgggagga ctgatggcat
     6601 ataccacgcc gctggtaaca ctctcatttt taatcaccac cccggaggca tgggtacccg
     6661 cattgggtga cgctcatcat ggtttccctt atttatccgg tgctggtcgc ttggtattga
     6721 aagatactct gatgctggca ggtgctgtca tgataatggc agattcggcg cgggaaattc
     6781 ttaaacaacg cagtaatgaa tccagttcaa cgttaaaaac tgaatattga taatccgaac
     6841 actgtctgtt gttcactttt tcaggcgtca ggcccccttt ctgatgatga agcctgacgc
     6901 catattaccg gagatatgac agagtgaaga tcaatatcac tcccctgtta aaagattctt
     6961 gtcgtctcag gtaaaatata tgattaatat caatcatgta tttttacagc aagacaacca
     7021 cgaacaaatt caggattaaa atgttttacc gactccccaa cataacccaa atcaagcgaa
     7081 ctaatttgtg ccatttcttt ctctgtcagt gagaaatccc agatagcaaa attttcttcg
     7141 atacgctgtt gatgtgtcga tttcggaata acgaccactc cccgttgaat attccaacgc
     7201 agaatgactt gagaaatcga tttttgatgg gcatctgcaa tactctgaag cagattattt
     7261 tcaaagggtt tatgtcgtcc accacctaac ggagcccatg cttcaggctg cacgttataa
     7321 tacttcatgg ttgccagcgc ctctggttgg gcaaaatagg gatgcaactc gacctggttg
     7381 accatcggtt taactcttac cgtttcacaa aagttcgcca gaacatgagg atagaaattg
     7441 gaaaccccaa ttgcttttaa tttgccctct tcataggcat cttcgagtgc acgccacaca
     7501 ctgaaataat cgcgcatagc ctggtgcaat aaatagaggt cgaagtactc tagtcccgat
     7561 ttttttaatg atgcttcaat acctgctgct gccgtatcct gattcaacat atcctgcccc
     7621 cacaatttag acgttataaa taactcatct cgggtacata aaccttcaga tatagcttca
     7681 cgaacggctt caccgacggc atcttcatta ccgtataccg ctgcagtgtc gatgagtcga
     7741 taacccgtgc ggatagcgtt caacactgac tgtttgcaca cgtttttatc ggtaacctga
     7801 aaaacaccaa atcccatcat cggcattttc agattgttac ttaatacgga aaattccact
     7861 gtcagtccct ccattaggca tgaacaatga gtctacgtta aaacataacc tcaaattagt
     7921 atgtggattt tgatatcact tatgcaaaaa attcattaat aatgtaggac tgaaacctct
     7981 ctattttcgg ggacaacgaa gcagacgcta ccagtgcttt tgccttcgac cttgctgttt
     8041 ttgatacact tagggccttg ggcataacga aaatgtgcga tatcacgaag aattaatgga
     8101 gaatg
//
LOCUS       DAZPJR010000180         7990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPJR010000180 DAZPJR010000000
VERSION     DAZPJR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.184"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            167..321
                     /locus_tag="ACHVB7_003716"
                     /pseudo
     CDS             167..321
                     /locus_tag="ACHVB7_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            367..783
                     /gene="hicB"
                     /locus_tag="ACHVB7_003717"
     CDS             367..783
                     /gene="hicB"
                     /locus_tag="ACHVB7_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR1915597.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            862..2268
                     /locus_tag="ACHVB7_003718"
     CDS             862..2268
                     /locus_tag="ACHVB7_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFR1915598.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2513..3658
                     /gene="ydcS"
                     /locus_tag="ACHVB7_003719"
     CDS             2513..3658
                     /gene="ydcS"
                     /locus_tag="ACHVB7_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR1915599.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3676..4689
                     /locus_tag="ACHVB7_003720"
     CDS             3676..4689
                     /locus_tag="ACHVB7_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1915600.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4690..5590
                     /locus_tag="ACHVB7_003721"
                     /pseudo
     CDS             4690..5590
                     /locus_tag="ACHVB7_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5580..6374
                     /locus_tag="ACHVB7_003722"
     CDS             5580..6374
                     /locus_tag="ACHVB7_003722"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1915601.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6396..7820
                     /gene="patD"
                     /locus_tag="ACHVB7_003723"
     CDS             6396..7820
                     /gene="patD"
                     /locus_tag="ACHVB7_003723"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR1915602.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         1860 a         1892 c         2203 g         2035 t
ORIGIN      
        1 attattcagt tttgcaaatt agcgcaaaga aattctggaa tcttccttcc tgattttgca
       61 ttgcattctg ccgttgcggc gatttagtgc tattttcgag caaattacac ataggggtaa
      121 acactaatgt ttactttatt gatatactca gcggcaggga ggcgatgtga aacaaagcga
      181 gttcagacgt tggctcgaat ctcagggcgt cgatgtagcg aatggccgca accatttgaa
      241 actcaggttt catgggaggc gcagtgtcat gccgcgtcac tcctgcgtaa agcaatcctg
      301 aaacaactcg gtttgagtta atcgccaatt aaaaaggtta atgacatgcg agagacagtc
      361 gaaattatgc gttatcccgt cactcttaca cccgcgccgg aaggcggtta tatggtttct
      421 tttgtggata tccctgaagc gttgacgcag ggcgaaactg tcgctgaagc gatggaagcg
      481 gcaaaagatg ctttactgac cgcatttgat ttttattttg aagataacga gcttatcccg
      541 ttaccttcgc cattaaatag tcatgatcac tttattgaag tacctttgag cgtcgcctct
      601 aaagtattgc tgttaaatgc ttttttacag tcagaaatca ctcagcaaga gttagccagg
      661 cgaattggca aacctaaaca ggagattact cgcctattta acttgcacca tgcgacaaaa
      721 atcgacgccg tccagcttgc ggcaaaggcg cttggcaaag agttatcgct ggtgatggtt
      781 taattacagt taacgaaaag ttgtcatttt taacaactga tatagactgc cgaatcatct
      841 gcacataatt acgattcgat aatgaaaaaa taccagcagc ttgcagaaca attacgcgag
      901 cagattgcgt cgggtatctg gcaacccggc gatcgtttgc cttcgttgcg tgaccaggtg
      961 gcgctttcag gcatgagctt tatgactgtc agccatgcct atcagttgct cgaaagtcag
     1021 ggatatatta tcgcacgacc gcagtcgggt tattacgttg cgccacaggc aataaaaatg
     1081 ccgaaagcgc cagtcattcc agtcactcga gatgaagcag tcgatatcaa cacttatatt
     1141 tttgatatgt tgcaggccag tcgcgaaccg tcggtcgttc cgtttgcctc ggcctttccc
     1201 gacccgcgac ttttccccct ccaacaacta aaccgctcgc tggcgcaggt aagcaaaacc
     1261 gccacagcga tgagcgtgat tgaaaactta ccgccaggaa acgcagaact gcgtcaggct
     1321 attgctcgtc gctatgcctt acagggcatc accatttctc ctgatgaaat tgtcattact
     1381 gccggggcgt tagaggcatt aaacctcagt ttgcaagcgg taactgaacc gggcgattgg
     1441 gtgatagtag agaatccttg tttctacggt gcgttgcagg cgctggagcg gctacggctg
     1501 aaggcgttat cggtggcgac ggatgttaaa gaagggatcg atcttcaggc gctggaactg
     1561 gcgttgcagg attatccggt gaaagcgtgc tggctgatga ctaatagcca gaacccactc
     1621 ggatttacct taacgccgca aaaaaaagca caactggtgg cgttactcaa tcagtacaac
     1681 gtaacgctga ttgaagatga cgtttacagc gaactttatt ttggacggga aaaaccgctg
     1741 cctgcgaaag cgtgggatcg ccacgatggc gttttgcatt gctcttcgtt ttcgaaatgt
     1801 ctggtgcctg gttttcgtat tggttgggtc gccgccggaa aacatgcacg taaaattcaa
     1861 cgcttgcagt tgatgagtac gctttccacc agctcaccga tgcaacttgc gctggtggat
     1921 tacctttcca cgcgccgata cgacgcccat cttcgtcgcc tgcgtcgcca gcttgcggaa
     1981 cgtaaacaac gtgcctggca ggcactgctg cgttatctgc ctgcggaagt taaaattcat
     2041 catagtgaca gcggttattt tctctggctg gagctccccg agccgttaga tgccggcgaa
     2101 ttaagcctgg cggcactgac gcatcatatc agtattgcgc cgggtaaaat gttttctacc
     2161 ggtgaaaact ggtcacgttt ttttcgtttt aataccgcgt ggcagtgggg agaacgtgaa
     2221 gaacaggcgg taaaacaatt aggcaaactt attcaagaac ggctgtaata gcgtttaatt
     2281 taattcctct tagattgggt aatatgaatt tcgaatagca gtcatatttc ctaacccctt
     2341 gtctatactc cagaagataa ccttacagac ggcataatgc gcggtagctc acaacctgat
     2401 taaattttct cagggacgaa ggtgtgcctg caagccgccg tctatggtta aacaaggaga
     2461 tatttttacg gcacggcggc tgaacaatta attacgacag gagtaagacc ttatgagcaa
     2521 gacatttgcc cgcagcagcc tgtgtgcgct caccatgaca ataatgaccg ctcacgccgc
     2581 cgaaccacct accaatttag ataaaccgga agggcgactg gatattatcg cctggccggg
     2641 atacatcgaa cgcggacaaa ctgataaaca atacgactgg gtaacgcaat ttgaaaaaga
     2701 gacaggctgc gcggtgaatg tgaaaaccgc cgcgacttcc gatgaaatgg tcagtctgat
     2761 gaccaaaggg ggttacgatc tggttacggc atccggcgat gcctcgctgc gtttgattat
     2821 gggcaaacgc gtgcagccga ttaataccgc cttgattccc aactggaaaa cgctcgatcc
     2881 gcgcgtggtt aaaggcgact ggtttaatgt tggcggcaaa gtttacggca caccttacca
     2941 atgggggccg aacctgctga tgtacaacac taaaaccttc ccgacgccgc cggatagctg
     3001 gcaagtggtt tttgttgagc aaaatctgcc ggacggcaag agcaataaag gccgcgttca
     3061 ggcttatgat ggccctatct acattgcgga cgctgcgttg ttcgttaaag ccactcagcc
     3121 gcagttgggc atcagcgatc cgtatcaact aaccgaagaa cagtaccagg cggtgctgaa
     3181 agtgctgcgc gctcaacaca gtttgatcca tcgctactgg catgacacta ccgtgcaaat
     3241 gagcgatttc aaaaacgagg gtgtggttgc ttccagtgcc tggccctatc aggccaacgc
     3301 cctgaaagcc gaaggccagc ctgttgctac cgttttcccg aaggagggtg ttaccggttg
     3361 ggctgatacc accatgctgc atagcgaagc gaaacatccg gtttgcgcct acaaatggat
     3421 gaactggtca ttaacgccaa aagtgcaggg cgatgtggcg gcctggtttg gctcgttacc
     3481 ggtagtgccg gaagggtgta aagccagtcc gttattaggc gaaaaaggtt gtgaaaccaa
     3541 cggttttaac tatttcgaca aaatcgcctt ctggaaaacg cctatagcag aagggggcaa
     3601 gtttgttccc tacagtcgct ggacgcagga ttacattgcc attatgggcg gtcgctaact
     3661 tcgctggggt gctttatgac gtacgcagtg gagtttgaca acgtctcgcg gttgtacggt
     3721 gacgtgcgcg cagtagatgg cgtcagtatt gcgataaaag atggtgagtt cttctctatg
     3781 ctggggccgt ccggctccgg caaaaccacc tgcctgcgcc tgattgctgg cttcgaacag
     3841 ctttccggcg gggctatctc tatctttggt aaacccgcca gcaatctgcc accgtgggag
     3901 cgggacgtga atactgtctt tcaggactac gcgctatttc cgcatatgtc gattcttgac
     3961 aatgtcgcct atgggctgat ggtcaaaggc gtgaataaaa agcagcggca cgcaatggcg
     4021 caagaggcgc tggagaaagt ggcgttgggg tttgtacagc aacgtaaacc gtcacaactt
     4081 tctggtggtc agcgccagcg ggttgctatc gccagagcat tggtgaatga accgcgcgta
     4141 ttgctgttgg atgaaccgct cggcgcactg gatctcaaat tgcgtgagca gatgcagctg
     4201 gaactgaaaa aactgcaaca gtctctcggt atcactttta tcttcgttac ccacgatcag
     4261 ggcgaagcgt tatcgatgtc cgatcgtgtg gcggttttca ataatggacg cattgagcag
     4321 gtcgattccc cgcgcgatct ctatatgcgc ccgcgcacgc cgtttgttgc cgggttcgtt
     4381 ggtacatcga atgtttttga tggactgatg gcagagaaac tttgtggcat gacgggaagc
     4441 ttcgccctgc gaccggaaca tatccgcctc aacacccctg gtgaactgca ggccaatggc
     4501 acgatccagg cggtgcaata tcagggcgcg gcaactcgtt ttgaactgaa attgaacggc
     4561 ggtgaaaaac tgcttgtgag tcaggccaat atgacaggcg aagaactgcc tgccacgctc
     4621 acgcccggac aacaggtgat ggtttcctgg tcgcgtgatg tgatggtgcc gctggttgag
     4681 gagaggtgaa tggcgatgaa tgtattgcaa tcaccttcac gtccaggtct ggggctgttt
     4741 ttactgctgc ttggcccgct aatgtggttt ggcattgtct atttcggctc gctgctgaca
     4801 ctgttatggc agggatttta tacttttgac gatttcacca tgtcggtaac gccggaactg
     4861 acgctggcga atatccgtgc gctgtttaat ccggcgaatt acgacatcat tctccgcacg
     4921 ctgactatgg ctgtggcagt cactatcgcc agcgccattc tggcttttcc aatggcgtgg
     4981 tatatggcgc gctataccag cgggaaaatg aaagcgtttt tatattgcgg taatgttgcc
     5041 gatgtgggcg agctacattg ttaaagccta tgcctggacg ttattgctgg caaaagatgg
     5101 cgtggctcag tggtttttac aacatcttgg gctggaacca ctgctgactg cgttccttac
     5161 attacctgcg gtgggcggaa atacgctgtc aacttccggg ctggggcgct ttctggtgtt
     5221 tctctatatc tggttgccgt tcatgatcct gcccgttcag gcggcgcttg agcgtttgcc
     5281 gccgtcattg ttgcaggcgt cggctgatct cggcgcacgt ccacgacaaa cctttcgcta
     5341 tgtggtgctg ccgctggcaa tcccgggtat tgccgctggc tctatcttta ccttctcact
     5401 cactctgggc gattttatcg tcccgcagct ggttggtcct ccaggatatt ttatcggcaa
     5461 tatggtttat tcccagcagg gggcgattgg caatatgccg atggcggcgg cattcacctt
     5521 ggtgccgatt attctcatcg cactgtacct ggcgttcgtg aaacgtctgg gagcgttcga
     5581 tgcactctga acgcgcaccg tttttcctca aactggcggc ctggggcggc gttgttttcc
     5641 tacattttcc catcctgata atcgccgcct atgcgtttaa cactgaagat gcggcgttta
     5701 gttttccacc gcagggcctg acgctgcgct ggtttagcgt ggcagcacag cgtagtgata
     5761 ttcttgatgc cgtgacactg tcacttaaag tggcggcgct ggcgacatta attgcgctgg
     5821 tgttagggac gctggcagct gccgcgctgt ggcgacgaga ctttttcggc aaaaacgcca
     5881 tttcgctgtt actgctgctg cccattgcgc tgccgggcag cgtcactggt ctggcgttat
     5941 taaccgcctt taaaaccatc aatctggagc cgggattttt caccatcgtg gtcggtcatg
     6001 cgactttttg tgtagttgtg gtgtttaaca atgtcatcgc ccgttttcgc cgcacctcct
     6061 ggagtctggt tgaggcgtca atggatcttg gggccaatgg ctggcaaacc ttccgctacg
     6121 tagtgttgcc gaatctcagt tcggcgttac tggcaggagg aatgctggcg tttgccttgt
     6181 cgttcgatga aatcatcgtt acgaccttta cggcaggtca tgaacgaacg ttactgttgt
     6241 ggttgctcaa tcagcttggg cgaccgcgtg atgtaccggt aactaacgtg gtggcactgc
     6301 tggttatgtt ggtaacaacc ttgccgatcc tgggggcctg gtggctaacc cgcgaaggcg
     6361 acaatggtca ataaccactg atacaggaat atgctatgca acataagtta ctgattaacg
     6421 gagaactggt tagcggcgaa ggggaaaaac agcctgtcta taatccggca acgggggacg
     6481 ttttactgga aattgccgag gcatccgcag agcaggtcga tgctgctgtg cgcgcggcag
     6541 atgcagcatt tgccgaatgg gggcaaacca cgccgaaagt gcgtgcggaa tgtctgctga
     6601 aactggctga tgttatcgaa gaaaatggtc aggtttttgc cgaactggag tcccgtaatt
     6661 gtggcaaacc gctgcatagt gcgttcaatg atgaaatccc ggcgattgtc gatgtttttc
     6721 gctttttcgc gggtgcggcg cgctgtctga atggtctggc ggcaggtgaa tatcttgaag
     6781 gtcatacttc gatgatccgt cgcgatccgt tgggggtcgt ggcttctatc gcaccgtgga
     6841 attatccgct gatgatggcc gcgtggaaac ttgctccggc gctggcggca gggaactgcg
     6901 tagtgcttaa accatcagaa attaccccgc tgaccgcgtt gaagttggca gagctggcga
     6961 aagatatctt cccggcaggc gtgattaaca tactgtttgg cagaggcaaa acggtgggtg
     7021 atccgctgac cggtcatccc aaagtgcgga tggtgtcgct gacgggctct atcgccaccg
     7081 gcgagcacat catcagccat gccgcgtcgt ccattaagcg tactcatatg gaacttggtg
     7141 gcaaagcgcc agtgattgtt tttgatgatg cggatattga agcagtggtc gaaggtgtac
     7201 gtacatttgg ctattacaat gctggacagg attgtactgc ggcttgtcgg atctacgcgc
     7261 aaaaaggcat ttacgatacg ctggtggaaa aactgggtgc tgcggtggca acgttaaaat
     7321 ctggtgcgcc ggatgacgag tctacggagc ttggaccttt aagctcgctg gcgcatctcg
     7381 aacgcgtcag caaggcagta gaagaggcga aagcgacagg gcacatcaaa gtgatcactg
     7441 gcggtgaaaa gcgcaagggt aatggctatt actatgcgcc gacgctgctg gctggcgcat
     7501 tacaggacga tgccatcgtg caaaaagagg tatttggtcc agtagtgagt gttacgccct
     7561 tcgacaacga agaacaggtg gtgaactggg cgaatgacag ccagtacgga cttgcatctt
     7621 cggtatggac gaaagatgtg ggcagggcgc atcgcgtcag cgcacggctg caatatggtt
     7681 gtacctgggt caatacccat ttcatgctgg taagtgaaat gccgcacggt gggcagaaac
     7741 tttctggtta cggcaaggat atgtcacttt atgggctgga ggattacacc gtcgtccgcc
     7801 acgtcatggt taaacattaa gttagatgtt agtggctgtt ataggacggc cactatcgtt
     7861 ttaggttctg agtttaatgg cggttaagta ttagaatgat tacattaata actagcgtaa
     7921 aaattaggct aaatgtttga tggcgatttt tcatcctgtc acagttgatt agcataaggc
     7981 tttagagtat
//
LOCUS       DAZPJR010000181         7968 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPJR010000181 DAZPJR010000000
VERSION     DAZPJR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.185"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHVB7_003724"
     CDS             <1..239
                     /locus_tag="ACHVB7_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR1915603.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="ACHVB7_003725"
     CDS             279..1142
                     /locus_tag="ACHVB7_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR1915604.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHVB7_003726"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHVB7_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR1915605.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="ACHVB7_003727"
     CDS             2679..4037
                     /locus_tag="ACHVB7_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR1915606.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="ACHVB7_003728"
     CDS             4180..5130
                     /locus_tag="ACHVB7_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR1915607.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="ACHVB7_003729"
     CDS             5140..6522
                     /locus_tag="ACHVB7_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR1915608.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6738..6812
                     /locus_tag="ACHVB7_003730"
                     /pseudo
     CDS             6738..6812
                     /locus_tag="ACHVB7_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="ACHVB7_003731"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="ACHVB7_003731"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR1915609.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2019 c         2308 g         1722 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggcgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctg
//
LOCUS       DAZPJR010000182         7958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPJR010000182 DAZPJR010000000
VERSION     DAZPJR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.186"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            321..437
                     /locus_tag="ACHVB7_003732"
                     /pseudo
     CDS             321..437
                     /locus_tag="ACHVB7_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            812..1054
                     /gene="yiiF"
                     /locus_tag="ACHVB7_003733"
     CDS             812..1054
                     /gene="yiiF"
                     /locus_tag="ACHVB7_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR1915610.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHVB7_003734"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHVB7_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR1915611.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHVB7_003735"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHVB7_003735"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1915612.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHVB7_003736"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHVB7_003736"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR1915613.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHVB7_003737"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHVB7_003737"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1915614.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHVB7_003738"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHVB7_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR1915615.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1843 a         2057 c         2075 g         1983 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatata
//
LOCUS       DAZPJR010000183         7946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPJR010000183 DAZPJR010000000
VERSION     DAZPJR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.187"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            111..443
                     /locus_tag="ACHVB7_003739"
     CDS             111..443
                     /locus_tag="ACHVB7_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR1915616.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="ACHVB7_003740"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="ACHVB7_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR1915617.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /gene="yegR"
                     /locus_tag="ACHVB7_003741"
     CDS             complement(2281..2598)
                     /gene="yegR"
                     /locus_tag="ACHVB7_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR1915618.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3004..3903
                     /gene="yegS"
                     /locus_tag="ACHVB7_003742"
     CDS             3004..3903
                     /gene="yegS"
                     /locus_tag="ACHVB7_003742"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR1915619.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3985..4758)
                     /locus_tag="ACHVB7_003743"
     CDS             complement(3985..4758)
                     /locus_tag="ACHVB7_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1915620.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="ACHVB7_003744"
     CDS             complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="ACHVB7_003744"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1915621.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5952..7307)
                     /locus_tag="ACHVB7_003745"
     CDS             complement(5952..7307)
                     /locus_tag="ACHVB7_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR1915622.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="ACHVB7_003746"
     CDS             complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="ACHVB7_003746"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR1915623.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7626..>7946)
                     /gene="gatA"
                     /locus_tag="ACHVB7_003747"
     CDS             complement(7626..>7946)
                     /gene="gatA"
                     /locus_tag="ACHVB7_003747"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR1915624.1"
                     /translation="AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQAD
                     DDNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVL
                     DSDE"
BASE COUNT         2148 a         2037 c         1784 g         1977 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctagtgtt
     2641 taaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
     2701 acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa ctccatgggg
     2761 gctttaatat attatattga agatgccact gtttagttga atattaggta tatgctcttt
     2821 tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg
     2881 taaccactga atcctccata cttgaactga ctttttatcc tccgactttc atcctgttct
     2941 gactccacct tttgttttct gctctacact atctacagac caatcataaa ggcacatacg
     3001 atcatggcag aatttcccgc cagcttactg attcttaatg gcaaaagtac tgacaatcta
     3061 cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc
     3121 acctgggaga aaggcgatgc cgcacgatat gtagaggagg cccggaagtt gggcgtcgca
     3181 acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc gttgattcag
     3241 tgtgaggggg atggcatacc cgcgctggga attttgccat taggaaccgc caatgatttt
     3301 gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc aattgccggt
     3361 aacgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat taatatggcg
     3421 acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa agccgcgctg
     3481 ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca accggaccgt
     3541 tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat tggtattggt
     3601 aacgggcgtc aggccggtgg cggtcagcaa ttgtgtccga acgcgttaat taacgatggc
     3661 ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt atcaacctta
     3721 aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa
     3781 gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagcggaca aaactttcat
     3841 attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtct attattgcgt
     3901 taatcaaact attctctctt gaaccaggga gaatccgaaa tgccggatgc ggtgtaaata
     3961 ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg tcttctgcaa
     4021 tgccagttta tccacctctg atacagcatc atcggtaata ataatgtcaa ttttttcgat
     4081 tggcagtacc tgattaaaac cgcgacggtt aaatttcgat gaatcgagaa cggcaatcac
     4141 tttattagcg gcggtgacca tcgcaccgct aatcgaataa ccttcattaa aggtcgtaat
     4201 accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat taatatcctg
     4261 caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga ccgtgccacc
     4321 acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg ggagactgtt
     4381 ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca ttgtcgtact
     4441 tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg cctgcgctat
     4501 tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt cctgggtctc
     4561 ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc
     4621 tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag caaatactcc
     4681 cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga tcttattcct
     4741 tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg taaacgaagg
     4801 gggaccattg gtgaataatc tgttttgcac aataccgggc cagcatcacg ctggcccgcg
     4861 gtttcatgga atgagcaaca ctttgcccgg catggcatta cgagcgatgt cacgcaccgc
     4921 ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc ttaacttacg
     4981 ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc tggaatagtt
     5041 catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt
     5101 taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag gaccggcaat
     5161 ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa gctgattaaa
     5221 gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac tgttaaatgt
     5281 ttgcatcgca ccgaaagatt ttgccaatgc cagtttttct gagctaatgt cgatcgccgt
     5341 cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa tggttccggc
     5401 accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag catgcaggcc
     5461 aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg gtagagcaaa
     5521 gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc gcgagccaat
     5581 aaaatcatat tttgcgcact gggaataaaa ccctttcaaa cactctggat aagtaaaaca
     5641 gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa caccggatcc
     5701 caccgcatca atatagccgc taaattcatg gcctaacgtt attggataat aatgtgcacc
     5761 atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa tttttacccg
     5821 cacctcatcc tgatgtttaa tttcaggaat gacgctttct gcaacgcgca cgataccatc
     5881 agtatcattc accattgatt tcataaaaac tcctgattgt taagggggat aacctccccc
     5941 tcaggtaaaa attattctgc gagaacggtt ttctcttgtt taataaaacc acgcgctcta
     6001 cgccaggtca tgaaaatacc ggtaagataa attgcgccga taataatgaa accgggaata
     6061 ttttgcgtgg agaaaacctg aatcagtaac caggtaatgg gagaaccgcc ctgatccatt
     6121 gaagccacca tacccccggc ttttaacgcc ccagcattag ccgccagttg ggtgtgcaaa
     6181 ccaatagttt gcgtcgcgat ccacagggtg atgctcataa tgatgacacc cgagattaag
     6241 gtgcggaaca gatttccacg atgcaccgcg accgccatcg ccacgaagaa gccgatggtg
     6301 gcaagatcgc caaacggcag tacctgatta cccggaacac aaacagcaat taaaatggtg
     6361 agcgggataa aaatcaggct tgccgatacc accgccgtat gccccagcag taatgcggga
     6421 tccaggccaa tcaggaactc ctgaccgccg aacttcgcct gtaaacgact acgggcctgc
     6481 ttagcgatgg gcgttaaacc atccatgatg ggtttaatca cccgtggcat cagcagcata
     6541 actgccgccg tttttaccgc cagctgcaat acacctttga catcgtaacc ggcgaggatg
     6601 ccgataatca gccccatcac aaaacccacg gtgacaggct cgccaaatgg accaaatttg
     6661 cgctgaatat cgtcggcgct aaatttaatt cggttaacgc ctgggatttt ctcgatgata
     6721 gcatcgacca gcaccgcaat cggccccata tacgccgacg taccgtgcgg aatagcaata
     6781 ccttccagct caaagaaatt tcgggtatcg cgggcaaacc agtcgccgag cttataaaca
     6841 aacgccgcgt gaattaccac acctgccatc cctatcatcc atgaaccggt tgccagatgc
     6901 agcaacgcgc cggtgaaggt catatgccag atattccaga tatcaacatt taccacccgc
     6961 gtcatacggg tcagtaacat cgccacgtta accagaatcg caatcggaat cgccaccagc
     7021 gcaatttgcg acgcccaggt cattggtgaa gagcccggcc agccgacatc gaccacatgc
     7081 agattcaggt cgaaattttc cgccatcgct ttcgccgccg gaccaatgga atccagcatt
     7141 aagccaatca caaggccaat gccaacaaag ccaatcccga tatgcagacc cgctttaaag
     7201 caatcgcctg ccttcatgcc taatatttta gaaaaaataa tgatgacaat cggaagcatc
     7261 accgtagggc cgaggtcgag aatataacgc atgacttctg aaaacatagg tcacccctgt
     7321 aagatagtca gaattttatt ttgtaatgct tcgataccga caccagaaat aaaaggcatg
     7381 ccgtgaacta acggaatatc gccaaaacta cgatccactt tggcagtggt gcatatcaaa
     7441 tgcacaccat ccatataggt ttctatttca ttaacccgac actggattaa ttcaacagga
     7501 atactatgat tctgacacaa ctctttaatt tcttccgccg ccatcgtaga ggtcgcaacc
     7561 gcgcctccgc aagcgacaat aatcttgcgt ttcataaata gtccttttta ttgttacaga
     7621 gggatttatt cgtctgaatc taaaacatac tttgtgaagt attcctttaa ctgggtttca
     7681 ggaagagtga ttagtgtctc gacgatatcg ggctgttgta acttgccaaa taaacagcgt
     7741 aaaagtttca attgctgctg cggattctcc acaatcaacg caataaccaa cgatacctcc
     7801 acgtcgttat catcatccgc ttgctgaaaa tgaactttat ttgttggcct taacagataa
     7861 atggctgacg acttagcatg aatcgcctca caatgcggta ttgcaatggc gtgctgctca
     7921 agcattatcc cggtagggaa ttctgc
//
LOCUS       DAZPJR010000184         7945 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPJR010000184 DAZPJR010000000
VERSION     DAZPJR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.188"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..359
                     /locus_tag="ACHVB7_003748"
     CDS             <1..359
                     /locus_tag="ACHVB7_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR1915625.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
     gene            complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHVB7_003749"
     CDS             complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHVB7_003749"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR1915626.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3127..3960
                     /gene="ppsR"
                     /locus_tag="ACHVB7_003750"
     CDS             3127..3960
                     /gene="ppsR"
                     /locus_tag="ACHVB7_003750"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR1915627.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4117..5163
                     /gene="aroH"
                     /locus_tag="ACHVB7_003751"
     CDS             4117..5163
                     /gene="aroH"
                     /locus_tag="ACHVB7_003751"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR1915628.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5295..5486
                     /gene="hemP"
                     /locus_tag="ACHVB7_003752"
     CDS             5295..5486
                     /gene="hemP"
                     /locus_tag="ACHVB7_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR1915629.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5490..6926)
                     /gene="selO"
                     /locus_tag="ACHVB7_003753"
     CDS             complement(5490..6926)
                     /gene="selO"
                     /locus_tag="ACHVB7_003753"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR1915630.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="ACHVB7_003754"
     CDS             complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="ACHVB7_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR1915631.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2030 a         2086 c         1888 g         1941 t
ORIGIN      
        1 ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc cgtgaagtag
       61 aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca atgcccgatg
      121 aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat cattcattat
      181 cgctggaaga ggtggtggct ttttttagcc gtaaacgggt cgcaaaatat aaatatcctg
      241 aacatatcgt ggtaatcgaa aaactaccgc gtactgcctc aggtaaaata caaaagtttt
      301 tgttaagaaa agatattatg cggcggttaa ctcaggatgt ctgtgaagag attgaataag
      361 tttcatcttc ggggatcaca taaccccggc gactgaacgc cgccggggat ttattttatt
      421 tcttcagttc agccaagctt aaccaggttt gcaccacggt gtccgggttc agagacaggc
      481 tatcgatccc ctcttccatc aaccatgcgg caaagtcttc gtggtcggac ggaccctgac
      541 cgcaaatccc gacatatttg ccctgtttct tcgcggcacg gatagccatc gacagcagtg
      601 ctttcaccgc atcgttgcgc tcatcgaaca attcagacac cacgccggag tcacggtcca
      661 gaccgagcgc cagctgcgtc atatcgtttg agccaattga gaagccgtcg aaatattcga
      721 ggaactgctc ggccagcaag gcgttggacg ggatttcaca catcatgatg attttcagcc
      781 cgttctcacc acgcttcagc ccctgacgcg ccagttcctc aaccaccgct ttcgcctgat
      841 ctacggtacg cacgaacggg atcatgatct caacgttggt cagccccatg tcgttgcgca
      901 cacgtttcac tgcttcgcac tccagcgcga agcagtcgcg gaagctgtcg gaaacatagc
      961 gtcccgcgcc acggaagccg agcatcgggt tctcttcatc tggctcgtaa cgctcaccac
     1021 cgaccaggtt ggcatattcg ttcgatttaa aatcagagag acggacaatg acgcgcttcg
     1081 gataaaacgc ggcacccagc gtcgcgatcc cttcagtcag acgaccaacg taaaattcac
     1141 gcggagaatc aaaacctttc atcatctcgc ggatttcatt ttgcaactgc ggttcctgat
     1201 cgtcaaactc aagcagtgcg cgtgggtgga cgccaatcat acggttgatg ataaattcca
     1261 gacgcgcaag gcccacgcct tcgttcggca ggcaggcgaa gtcgaaagca cggtccgggt
     1321 taccgacgtt catcatcact ttcaacggca gatccggcat catttctacg ctggagcttt
     1381 tcacgctaaa ttccagcaac tccgcataga cgtaaccggt atcaccttcg gcacaagaaa
     1441 cagtgacgtt ctcaccgtct ttcatccgtt ctgttgcatc accacagccc actaccgccg
     1501 gaatgcccag ttcacgagcg atgatcgccg cgtgacaggt acgaccgcca cggttggtga
     1561 cgatggcaga tgctttcttc atgatcggtt cccagtccgg gtcggtcatg tcagtgacca
     1621 gcacgtcacc aggttcgatg cggttcattt cgctgatctc atggatgact ttcaccggac
     1681 ccgcgccgat gcgatgaccg atagcacggc cttcggcgat aatcttaccc tgtgaatgca
     1741 gcgtataacg ctccatgacc tgaccgcgtg agcgcacggt ttccggacgc gcctgcacaa
     1801 tgaacagttt gccagtgtgg ccatctttcg cccactcaat atccatcggg cgaccgtagt
     1861 gtttctcaat ttgtacggcc tgttttgcca gttcctgcac ttcttcgttg gtcagcgaga
     1921 agatgtcacg ctgttcctgc ggtacgtctt cgattttaac ctgcttgccg tgctcctggg
     1981 tcggcgcgta aaccatgcgg atttttttcg accccatggt gcgacgcacg atagccgggc
     2041 gattcgccgc cagtgtcggt ttatggacgt aaaactcatc cgggttaacc gcaccctgca
     2101 cgaccatttc cccaaggccc catgcggaag tgataaacac cacctggtca aagccggatt
     2161 cggtatcaat ggagaacatc acgccagaag atgcgaggtc ggagcgcacc atccgctgaa
     2221 caccggcaga gagcgccacg ccacggtgat cgtaaccctg gtgcacacga taagagatgg
     2281 cgcgatcgtt aaacagagaa gcaaatacat gcttcactgc cacgagaacg gcgtcaaaac
     2341 cctgaacgtt gaggaaggtt tcctgctgac cggcaaaaga agcgtccggc atatcttctg
     2401 cggtggcgga ggagcgcacc gcaaaagagg cgttttcgtc atcggcggaa agctgtgcat
     2461 aggcttcgcg gatggcgttt tccagctcag gctggaaggg agtgtcgata atccactggc
     2521 ggatttgcgc gcccgctttc gcaagctgag taacatcgtc aatatccgtt ttatccagca
     2581 gttcataaat gcgctggttt acgccgcttt ggtccagaaa ctggttaaac gcgtcggcgg
     2641 ttgtggcgaa accattcgga acggaaacac ccattccgga aaggttagta atcatttcac
     2701 ccagggaggc atttttgccc ccaaccctgt ctacatcatt catgccgagt tggttatacc
     2761 aaagcaccag cggtgacgag ccattgttgg acatcgaaca atccttttgt gataaatgaa
     2821 cggtttgaga aacacatttc tgcgcattta tctttgcata tttaaccgga tgaaaaaaac
     2881 ggtgaatcgt tcaagcaaat atattttttt actttttaag actgatccca gcgttgcgca
     2941 aatctgctct cttcgacaat ttccacataa accatcggta taaacgaaac cataaaaatg
     3001 aaatgctgtt ttcataaaaa ataaaattga aggttcattt tataaaccag acataacgtc
     3061 ctacgcttct gtgcgttttt aatttatgct ttcatagaat tatgtctgca tcacgggaag
     3121 aacaaaatgg ataatgctgt tgatcgccac gttttttata tttctgatgg tacggcaata
     3181 actgcggagg tattaggaca cgcagtaatg tcacaatttc ccgtcactat cagcagcatt
     3241 acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca gattgacgca
     3301 atttatcacc agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt gccggagatt
     3361 cgcgccatca tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc gctggttgcc
     3421 ccgctacaac aagagatgaa actggatcca acgccgattg ctcatcgtac ccatggcctt
     3481 aaccctaata atctcaataa atatgatgcg cgcattgcgg cgattgatta caccctcgcc
     3541 cacgatgacg gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt
     3601 tctcgctgtg gtaaaacccc caccagtctt tatctggcaa tgcaatttgg tatccgcgcg
     3661 gcaaactacc cctttattgc cgacgatatg gataatctgg tgctacccgc gtcgctcaaa
     3721 ccgcttcagc ataaattgtt cggcctgact atcgacccgg aacgtctggc ggcgattcgc
     3781 gaggaacgtc gggagaacag tcgctatgcc tcgcttcgtc agtgcaggat ggaagtcgcg
     3841 gaagtggaag cgttgtaccg taaaaatcag atcccgtgga ttaacagtac caattattcg
     3901 gtagaagaga ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg aatgtactag
     3961 agaactagtg cattagctta tttttttgtt atgatgctaa ccacccggcg aggtgtgaca
     4021 cacctcgcac ttgaaatcag cagcgattgg tttatcgtga tgcgcatcac ttcccggcag
     4081 tcctgccgta gaagcaacaa atttctgaga cttgtaatga acagaactga cgaactccgt
     4141 actgcgcgta ttgagagcct ggtaacgccc gccgaactcg cgctacggta tcccgtaacg
     4201 cctggcgccg ccacccatgt caccgactcc cgccgcagaa ttgaaaaaat actcaatggt
     4261 gaagataagc gactgttggt cattattggc ccctgctcga tccacgatct caccgctgca
     4321 atggagtacg ccccccgtct gcagtcgctg cgcaaccagt accagtcacg gctggaaatc
     4381 gtaatgcgca cctattttga aaaaccacga actgttgtcg gctggaaagg actaatctcc
     4441 gatccagatt taaacggcag ctatcgggta aatcacggtc tggagctggc gcgcaaatta
     4501 cttttacagg taaatgagct gggcgtccca accgcgactg agttcctcga tatggtgacc
     4561 ggtcagttta ttgctgattt aatcagttgg ggcgcgattg gcgcacgtac taccgaaagt
     4621 cagatccacc gtgaaatggc ttcggcactc tcctgtccgg taggttttaa aaatggtacc
     4681 gatggcaata cgcggattgc tgtggatgct atccgcgcag cccgcgccag ccatatgttc
     4741 ctctcgccag acaaaaatgg tcagatgacc atctatcaga ccagcggcaa cccgtatggc
     4801 cacattatta tgcgtggcgg caaaaaaccg aattatcatg ccgatgatat cgccgcagcc
     4861 tgcgatacgc tgcacgagtt tgatttacct gaacatctgg tggtggattt cagccacggt
     4921 aactgccaga agcagcaccg tcgccagtta gaagtttgtg aggatatttg tcagcaaatc
     4981 cgcaatggct ctacggcgat tgctggaatt atggcggaaa gtttcctgct cgaaggaacg
     5041 caaaaaatcg tcggcagtca gccgctcact tacggtcaat ccattaccga cccgtgtctg
     5101 ggctgggaag ataccgaacg cctggtcgaa aaactcgcct ctgcggtaga tacccgcttc
     5161 tgaatgcgtg cccattcctg acggaatggg catttctgcg caacttgttg tcttctcaac
     5221 aaattactgc ttgctctggc ctgccataat attgataata agaatcattg ttatatcaat
     5281 tattattaat ctttatgcgt tatacggata gcagaaaact cacgcctgaa acggacgcca
     5341 atcacaaggc cgcttccccg cagcccatcc ggcgaattac cagccagaca ctgttaggtc
     5401 cggatggcaa actgattatc gatcatgacg ggcaagaata tctgctccgt aaaactcagg
     5461 ctggcaagct gctgttaact aagtagcctt taactcgagc agctgacttc cagccgttta
     5521 ccccagtcag gtggacgact gacatagtca tcatctctat cgctgaaagg atttcgcaac
     5581 gccccatgca ggcggtgcaa ttccgtcata tcaccctttt ctgccgcttc aatcgcccgt
     5641 tgcgccaacc agttgcgcaa taccagagcg gggttaacgc tttgcatcaa ctgctgacgc
     5701 tcactatcgc taacctcgtc ttgttgcaaa cgcccccgat aacgggcaaa ccagccatca
     5761 aatgccgcac gatcaataaa ctcatcacgt agcggtgacg ccgtgctgtg ctgctcggtc
     5821 agactcagca tgcggaatgt gcgggtataa tcgctgcgct ctcgcgccat cagactgaat
     5881 aattcattca gtagcgcgtt atcctctttt tgctccgtga tgaagcccag tttctgccgc
     5941 atccgttgtc cataatgcgt caacaaaacc tgctgatagc tgtccagggc ctcattcagg
     6001 gcatctacgg caacaaatgg tgacaatgtc tgcgccagac gctgtaaatt ccacaacgcg
     6061 acagcaggtt gattatcaaa actataacgc ccttgatgat ccgagtgatt acaaataaaa
     6121 ccgggttcgt aatcatcaag aaaaccaaac ggcccgtaat caagcgtcag tcccagcagc
     6181 gacatgttgt cggtattcat caccccatgt gcaaagccga ccgtctgcca ttgggcaatt
     6241 aacgacgcgg tacgtgcgac aacatcgcta aaccagagac ggtatttgtc ctcatcatct
     6301 tcaagatgtg accagtaatg acgaatagca aagtcagcta actgacgaac cttttccgac
     6361 tcgcggcggt agtaaaaatg ttcgaaatga ccaaagcgca gatgacttgg tgccacacgc
     6421 atcagcatcg cgcctggctc cgccgtttcc cgatacactg gcgaatcgct ggtgacgata
     6481 cttaacgcgc gggtcgtcgg aatgcccaga taatgcatcg cctcactggc gagactttct
     6541 cgtatcgtcg aacgtaaaac cgcccgtcca tcacccattc gcgaataagg cgtcaggcca
     6601 gcacctttca gatgccagtc cattgtagtg ccatcagcaa gcagttgttc gccgagtaaa
     6661 atgccgcgcc catcacccag ttggcccgcc cagacgccga actgatgacc actgtaaacc
     6721 tgcgccagtg gtgacatacc aggcagtagg gcttcgccgc cccagacacc tgcgccattt
     6781 ttaaacagcg acgatggaat actcagcgtg ttagccagtt cggtattatg ccaaatcagc
     6841 cgggcattat ttaaaggcgt aggggaaagt gctgtatagg tttctggcaa ttcatcgcgc
     6901 cagcgggtaa caaaagacag ggtcatagat cctcctgttt gatagtgtag acggttactc
     6961 tcgtcttaaa caccagcaaa cagaagggtt atcgctgaac caacgtcgtt atctgattta
     7021 tgggaacggc aggccacagg catccctgga gagcatggaa atcgaagggg gtaatctgtg
     7081 ccaatatttc tgccgtatca atgcctcccg caatgatgca attgcaacaa ggggaaattt
     7141 gcgcctggat cgcacgaata aacggttcga aggagcggtg cgtgatttgt tgctgaataa
     7201 aacttttatc cagcatgacg cgggtgaaaa gaccatcaaa aaccgctttc attgtgctat
     7261 tgcctgctcc caaattacct aacacaaggg gatacacttg cgataaggat aaaagaccgc
     7321 gattatcttt tccttcattg agatgcgggt aattctcatt aatcaacagt tcaataaacg
     7381 gatattttaa tagttcgcct gcgtaattat ccctctctaa taataatgta gccacttgtg
     7441 gcgttaaatt aagccaggca aagagtttat gttgaataaa aaaatgttgg catgatttta
     7501 gtaattccaa ctgctctgaa aataattgcc aatgttgctc ctcggtaagt tgcgctataa
     7561 cccgactcgt cgggatacgc accgtgccat cttcactgga gaaatgggta attaactcaa
     7621 cgccgacaag gacttgctgg ttatctctta tcggcaggaa atagcaatca gaatgataaa
     7681 gattctccaa aaaaatcttc atggtaaccg tccctctcga aggatgtttt tcagtatccg
     7741 gctaaaaggg atgaggccat aagacataac aattacagaa ggagtaactt tcatttgttc
     7801 catgttaacc actttttcag gggtcctttt tttagattat cctgattata aacgaataat
     7861 ccatttacgg aatttttgtc tgcaaaatac tactgtattc aggagtaaat ggacggcgat
     7921 gtatcatcgc cgtccatttt tcgac
//
LOCUS       DAZPJR010000185         7928 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPJR010000185 DAZPJR010000000
VERSION     DAZPJR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.189"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            63..1316
                     /locus_tag="ACHVB7_003755"
     CDS             63..1316
                     /locus_tag="ACHVB7_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR1915632.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(1386..2357)
                     /gene="rfaC"
                     /locus_tag="ACHVB7_003756"
     CDS             complement(1386..2357)
                     /gene="rfaC"
                     /locus_tag="ACHVB7_003756"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR1915633.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(2361..3407)
                     /gene="rfaF"
                     /locus_tag="ACHVB7_003757"
     CDS             complement(2361..3407)
                     /gene="rfaF"
                     /locus_tag="ACHVB7_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR1915634.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(3417..4349)
                     /gene="rfaD"
                     /locus_tag="ACHVB7_003758"
     CDS             complement(3417..4349)
                     /gene="rfaD"
                     /locus_tag="ACHVB7_003758"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR1915635.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4563..5759
                     /gene="kbl"
                     /locus_tag="ACHVB7_003759"
     CDS             4563..5759
                     /gene="kbl"
                     /locus_tag="ACHVB7_003759"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR1915636.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            5769..6794
                     /gene="tdh"
                     /locus_tag="ACHVB7_003760"
     CDS             5769..6794
                     /gene="tdh"
                     /locus_tag="ACHVB7_003760"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR1915637.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            7033..>7928
                     /gene="waaH"
                     /locus_tag="ACHVB7_003761"
     CDS             7033..>7928
                     /gene="waaH"
                     /locus_tag="ACHVB7_003761"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFR1915638.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         1988 a         1867 c         1916 g         2157 t
ORIGIN      
        1 aaaagaataa acgttagatc ttgcattaaa taatccgtcc tcgtagcata ggttgaaact
       61 atatgtcatt ttgttggaat gaaattaatt ctggtataaa gtctttaatt ctcatattat
      121 gtattttttc tttgatgact ttgtctttat gggatgatgt tgcaacaaag tttcttcatg
      181 cagctggaat tatatctgca ttgtattttc taataacacc aaaaaaaaca ataactaaca
      241 atcctacttt gttaattttc atctcattat gtcttttggg aatcataaat atcatctggt
      301 attcacatta taaagtttca ggctctgttt ataccaatgc atatcgtggc ccaatggaaa
      361 ctgggaaaat tgccttgtgt agtgctttta ttttcttggt tctttttgct aaaaatgagt
      421 tgagaacaaa aataaaattt gggaaactaa ttctgttcgc atccctggca acgcagttac
      481 ttttttttgc gcatgccatg tggcaacatt tctatttaaa cgttgaccgt gttgcattat
      541 cagcttccca cgctacaaca gcaggctaca tcatcctttt cccttcttta ctggcatcaa
      601 ttctcatttt aaaatccgac tttagacata aaacaacatt atatacaatt aacttcatgc
      661 ttagcttatg tgctgtcata gtaactgaaa cgcgtgcagc catattagtg tttccattct
      721 ttgcgttact attaatcgta atggatagtt atattaataa gcgaattaat tataagttat
      781 attgttttat tgcgattgca ttattagcag gtgtattttc ttttaaagat acattgctta
      841 tgagaatgaa taacttaaat aacgatttag ttaattattc gcatgataac accagaactt
      901 cagtcggtgc ccgtctggca atgtatgaag ttggcttaaa aacatattct ccaataggac
      961 aatcactgga aaaacgagcg gaaaaaatac atgaactaga agaaaaagag cctagattga
     1021 gtggcgcttt accctatata gattctcatt tgcataacga tctcatagat acgttatcaa
     1081 cgcgtggtat tcctggagtt gtattaacaa ttttagcctt ttcagcaata ctcatatatg
     1141 ccttaagaac tgctaaagaa ccttatattt taatcttgct tttttcacta ctggtagtag
     1201 gcctaagtga tgtaatactc ttttctaaac cggttccgac tgctgtgttt gtcaccataa
     1261 tattgctttg tgcttatttt aaagcacaat cagaccaatg tttattagat aaataatgaa
     1321 tatgtgaaat aaaatcagca aggaggctga ttttatttca aaattatcta ttaaggggtt
     1381 gtaatttaaa tcatagcaac gttttcttca atgaatcgct taacagcatt tgctgtcaat
     1441 tgagacaact cattccccgg agccctacaa accatctgat tcttcccata tccaccaatt
     1501 aatcccggat cggttggtcc ataaaccgtg atattgggtc tatccagtgc cgccgttaaa
     1561 tggcttaacc ccgtatccac cgacactaca aatttagccc cggccagcac gcgggcaacg
     1621 ccttccagac tcatcttcgg caatacttca acataagcaa atccttccgc cagtcgtttc
     1681 gcccgctctt cctcatgcgg cgcgccccac ggaagtttaa tccgtattcc tgaatcagcc
     1741 agtaaaccaa tcaattctcg ccagtgttct tccggccagt gtttatcatc acgggtcgtc
     1801 gcatgaagaa atacggcata ttcgccagca tctgtaggca ggttcgtcag gaaatgctgt
     1861 gcaatagcat aatcgccctg ggtttgcggt ttgctatagc ccagactttt ggcaaacagt
     1921 tcgcgggtgc gttctacggc gtgctgctgt tttgcaatat gatgcttacg attgtaaaac
     1981 agactggcta aaggttcgcg agcggtttgc cagtccatgc catgctttac gccatgcgcc
     2041 agacgcgtca ccagcgccgc gctttttacc agcccctgag cgtcgataac tgcgtcatag
     2101 ttctctgctt gtagcgcttc acgaaacgct ttgcgttccg cttttatggg ggccgagaac
     2161 caggctttac gccagcgacg tattgccaca ggaataactc gctcaacggc ggcgtgccag
     2221 gaaggaatct gtgcgaaccc ttcttccacc acccagtcaa acttaatccc tgggattgcc
     2281 tgctgggcat cagtgagtgc gggcaacgta tggagaacat cgcccatcga cgatgtttta
     2341 acgatcaaaa cccgcatccg tcaggcttcc tcttgtaaca atagcgcgtt gagttcttcc
     2401 agtacgcgct ggggagtaat gtcgattaag ctctggtgat aaccctccgc agcgtcacct
     2461 ttacgcactt tgtgatagcc ggtaatcagg cggatcacgc gcgctttatg ggatagcggc
     2521 ggtgtgaagt ccgggctact cggaccatac agggcaacca gcggacgatt gagcgccgcc
     2581 gcaacgtgca tcaggccaga atcgttagtg acaatggctt tacaggctgc aatcaggata
     2641 accgcttgat caagctgtgt ttcccccgcc aggttccgac accatgcctg ctgctcggta
     2701 ttcaaagcgg caagaatctc attgcccgct tcatgatctt tcgccgagcc aaacagaacc
     2761 acctgataac cttcatcaat cagctgcttt gccagttccg catagtggta gtgtggccag
     2821 cgttttgccg gaccaaactc cgcacccggg caaaaaccaa tcatcggacg ttctgatgaa
     2881 agcgaaaatt gattacaggt atatgatttt tcaccttcgc tcacctgcaa ttgcggccat
     2941 aacaacggtt ttggtagatc ttgtgcggta cgcatcacgc ctttgtcata ggccagcgcg
     3001 acatagcgtt ccaccattag cggccaggct tctttatcga gcacgcgtaa atcgttgagt
     3061 aaaccgtagc gcatctcgcc gcgccagccg gtgcgatgag gaatacccgc gaagaaaggc
     3121 actaatgcag atttgaagga gttgggtaag acgtaggcgc ggtcgtagcg cttttcacgc
     3181 aggctatgac ccagtttgcg gcgttcgccg atttccagcg ctccgtgacc gagaggcata
     3241 ggaatagctt cgttaacttc cggcatccgc gataataatg gacggcacca tgccggtgcc
     3301 atcacatcga ttatcgcctg gggatagcgc gcctggagcg tgcgatagag actttgcgac
     3361 atcatcatgt cgccaaccca agacgggccg atcaccagta ttttcatgca gagctcttat
     3421 gcgtcgcgat tcagccaggc catgtattcc gttacacctt cagcaacggt tttgaacggt
     3481 ttgtcgtaac ccgccgcgcg cagatttgtc agatctgcct gagtgaacgc ctggtagcgg
     3541 cctttcagct tatccgggaa cggaatgtat tcgatctggc ctttcttgtg ataagccagc
     3601 gtagcatcag ctacagcctg gaaggattcc gcacgaccag taccgaggtt gaagatgccg
     3661 gaaacgccat tttccaggaa ccacagattc acatcagcca cgtcgcccac atagacgaag
     3721 tcgcgtttga agttctcgct accttcaaac agcttcggtg attcaccgtt gttaagctgg
     3781 gtgttgagat ggaaagcgac gctcgccatg ctgcctttat ggccttcacg cggtccataa
     3841 acgttgaaat agcggaagcc aacaatctgc gagttagctt caggcaggat ttggcgaaca
     3901 tattcatcaa acaggaattt tgagtaaccg tagacgttca acggtttttc gtactcgcgg
     3961 gattcaataa agtcggaggt gcgtccgccg taggtggctg cggaagaggc atacaggaac
     4021 gggatttcgc gctccagaca gtagtgcagc agctctttgg agtattgata gttgttatcc
     4081 atcatatact tgccatccca ctcggtggtg gaagagcacg cgccttcgtg gaaaatcgct
     4141 tcgacatcgc cgaactcttc gccagccata atctggatca ggaagtcttc cttatccata
     4201 tagtccgcga tatccagatc caccaggttc acaaacttgg tgccgtcttt caggttgtcc
     4261 accaccagaa tatcggtgat gcctttatca ttcagggcct taacgatgtt gctgccgata
     4321 aagcccgcgc cgccggtaac gatgatcata actgtaacct tcgaattatg gagtcagaga
     4381 caatctcaga cacgaatact tctatcatat cactacacgg cgttggcttc agccattcgc
     4441 caaaagtgag attgatggac gttatttatc ctgaattttg cagaagtgtt aacgcgtcat
     4501 ctcgtcgcga cctataagtt tgggtaatat gtgctggaat ttgccctgtc tggagaatcg
     4561 caatgcgtgg agaattttat cagcagttaa ccaacgatct ggaaaccgca cgggcggaag
     4621 ggttgtttaa agaagagcgc attattacgt ctgcgcagca agcagatatc actgtggctg
     4681 atggaagcca cgtcattaac ttttgtgcca ataactatct cgggctggcg aattatccgg
     4741 atctgattgc ggcggcaaag gcgggaatgg attctcacgg tttcggcatg gcttcggtgc
     4801 gttttatttg cggcactcag gacagccata aagagcttga acaaaaactg gcggcattcc
     4861 tggggatgga agatgcgatt ctctactctt cctgctttga tgctaacggt ggcctgtttg
     4921 aaacgctgct gggcgcggaa gacgccatta tctccgacgc actgaaccac gcgtctatta
     4981 ttgatggtgt gcgtctgtgt aaagctaaac gctatcgcta tgccaacaac gatatgcagg
     5041 agctggaagc acgtctgaaa gaagcgcgtg aagccggtgc gcgtcatgtg ctgattgcca
     5101 ccgatggtgt gttctcgatg gacggcgtga ttgccaacct gaagggcgtt tgcgatctcg
     5161 cagataaata tgatgccctg gtgatggtag acgactccca cgcggtcggt tttgtcggtg
     5221 aaaatggtcg tggttcccat gaatactgcg atgtgatggg ccgggtcgat attatcaccg
     5281 gcacgctcgg taaagcgctg ggcggggctt ctggtggtta taccgcggcg cgcaaagaag
     5341 tggttgagtg gctgcgccag cgttctcgtc cgtacctgtt ttccaactcg ctggcaccgg
     5401 ccattgttgc cgcatccatc aaagtactgg agatggtcga agcgggtagc gagctgcgtg
     5461 accgtctgtg ggcgaacgcg cgtcagttcc gtgagcaaat gtcggcggcg ggctttaccc
     5521 tggcgggagc cgatcacgcc attattccgg tcatgcttgg tgatgcggta gtggcgcaga
     5581 aatttgcccg tgagctgcaa aaagagggca tttacgtcac cggtttcttc tatccggtcg
     5641 ttccgaaagg tcaggcgcgt attcgtaccc agatgtctgc ggcgcatacc cctgagcaaa
     5701 ttacgcgtgc agtagaagca ttcacgcgta ttggtaaaca actgggcgtt attgcctgag
     5761 gatgtgagat gaaagcgtta tccaaactga aagcggaaga gggcatctgg atgaccgacg
     5821 ttcctgtacc ggaactcggg cataacgatc tgctgattaa aatccgtaaa acagccatct
     5881 gcgggactga cgttcacatc tataactggg atgagtggtc gcaaaaaacc atcccggtgc
     5941 cgatggtcgt gggccatgaa tatgtcggtg aagtggtagg tattggtcag gaagtgaagg
     6001 gcttcaagat tggcgatcgc gtttctggcg aaggccatat cacctgcggt cactgtcgca
     6061 actgtcgtgg cggtcgtacc catctgtgcc gcaacacgat cggcgtcggc gttaaccgcc
     6121 cgggctgctt cgccgaatat ctggtgattc cggcgttcaa cgccttcaaa attcccgaca
     6181 atatctccga cgacctggct tccatttttg atcccttcgg taacgccgtg cataccgcgc
     6241 tgtcgttcga tctggttggc gaggatgtgc tggtctctgg tgcaggcccg attggtatta
     6301 tggcagcggc ggtggcgaaa cacgttggtg cacgcaatgt ggtgatcact gatgttaacg
     6361 aatatcgtct cgaactggcg cgcaaaatgg gtatcacccg tgcggtcaac gtcgctaaag
     6421 aaaaccttaa tgatgtgatg gcagaactgg gcatggccga aggctttgat atcggtctgg
     6481 aaatgtccgg tgcgccgcca gcgtttcgta ccatgctcga caccatgaac cacggcggtc
     6541 gcattgcgat gctgggtatt ccaccgtccg atatgtctat cgactggacc aaagtaatct
     6601 ttaaaggctt gttcattaaa ggtatttacg gtcgtgagat gtttgaaacc tggtacaaga
     6661 tggcggcgct gattcagtca ggtctggatc tctcgccgat cattacccat cgtttctcta
     6721 tcgatgattt ccagaagggc tttgacgcta tgcgttcggg ccagtccggg aaagtaattc
     6781 tgagctggga ttaacacgaa caagggctgg tattccagcc cttttgcctg aggataatcc
     6841 gttaaatatg taaaatcctg tcagtgtaat aaagatatcg taattgcgct gatctcttat
     6901 atagctgctc tcattatctc tctaccctga agtgactctc tcacctgtaa aaataatatc
     6961 tcacaggctt aatagtttct taatacaaag cctgtaaaac gtcaggataa cttcagaggt
     7021 cgtcggtaat ttatgatgaa cagcaccaat aaacttagtg ttattattcc gttatataat
     7081 gcgggcgatg atttccgcac ttgtatggaa tctttaatta cgcaaacctg gactgctctg
     7141 gaaatcatta ttattaacga tggttcaacg gataattctg ttgaaatagc aaagcattac
     7201 gcagaaaact atccgcacgt tcgtttgttg catcaggcga atgctggcgc atcggtggcg
     7261 cgtaatcgtg ggattgaagt ggcaacgggc aaatatgtcg cttttgtcga tgctgacgat
     7321 gaagtctatc ccaccatgta cgaaacgctg atgaccatgg cgttagagga cgacctcgac
     7381 gtggcgcagt gcaacgctga ctggtgtttt cgtgaaacgg gagaaacctg gaaatccatc
     7441 cccagcgatc gccttcgctc aaccggcgta ttaaccggcc cggactggct gcggatgggg
     7501 ctttcttcgc gccgttggac tcacgttgtc tggatggggg tttatcgccg cgatgttatt
     7561 gttaaaaata acattaaatt tattgccgga ttacatcatc aggatattgt ctggacaaca
     7621 gaatttatgt ttaatgcgct gcgtgcgcga tataccgagc aatcgttata taaatattat
     7681 ctgcataata cgtcagtgag tcggttgcat agacaaggga acaaaaacct taattatcaa
     7741 cgtcactata ttaagattac tcgtctgcta gagaaattaa atcgaaatta tgccgacaaa
     7801 attacgattt atccggaatt tcatcagcaa ataacttacg aagcattgcg tgtttgccat
     7861 gcggtgcgca aagagccgga tattattacc cgccaacgga tgattgccga gatatttact
     7921 tccggtat
//
LOCUS       DAZPJR010000186         7852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPJR010000186 DAZPJR010000000
VERSION     DAZPJR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.190"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHVB7_003762"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHVB7_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR1915639.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHVB7_003763"
     tRNA            1230..1305
                     /locus_tag="ACHVB7_003763"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHVB7_003764"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHVB7_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHVB7_003765"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHVB7_003765"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR1915640.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHVB7_003766"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHVB7_003766"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR1915641.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHVB7_003767"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHVB7_003767"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR1915642.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="ACHVB7_003768"
     CDS             complement(4869..5306)
                     /locus_tag="ACHVB7_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR1915643.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="ACHVB7_003769"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="ACHVB7_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="ACHVB7_003770"
     CDS             complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="ACHVB7_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR1915644.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT         2210 a         2083 c         1665 g         1894 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccctgatga
     6541 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     6601 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
     6661 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
     6721 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     6781 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
     6841 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
     6901 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
     6961 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
     7021 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
     7081 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
     7141 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
     7201 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
     7261 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
     7321 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
     7381 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
     7441 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
     7501 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     7561 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     7621 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     7681 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     7741 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     7801 ccaacgcaat taattcacat tgaaatatcc acaaaacaac caataaatta ac
//
LOCUS       DAZPJR010000187         7776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPJR010000187 DAZPJR010000000
VERSION     DAZPJR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.191"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHVB7_003771"
     CDS             complement(<1..1780)
                     /locus_tag="ACHVB7_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR1915645.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPEWLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHVB7_003772"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHVB7_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1915646.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHVB7_003773"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHVB7_003773"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1915647.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHVB7_003774"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHVB7_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR1915648.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHVB7_003775"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHVB7_003775"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR1915649.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHVB7_003776"
     CDS             complement(5968..7005)
                     /locus_tag="ACHVB7_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1915650.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHVB7_003777"
     CDS             7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHVB7_003777"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1915651.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc attcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaag ccagcacccg
     7141 gctggctaag taatactgga agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag
     7201 ggtcttcctg atcgcgagac aataataatc attctcattc gcacttgtcc aacacttttt
     7261 gcaaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg gacatttacc
     7321 ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg
     7381 aggccatcag gcagattcac gcccagccgg aggtgtattg caacacacta caggtgcctc
     7441 atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc aagcaactcg
     7501 tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa cagcacccac
     7561 gccgcagtca tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg
     7621 tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg cgggtagatc
     7681 gcattgaact aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg
     7741 gctttgaaat tgaagggact ggtaagaagt acgcat
//
LOCUS       DAZPJR010000188         7715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPJR010000188 DAZPJR010000000
VERSION     DAZPJR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.192"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHVB7_003778"
     CDS             <1..643
                     /locus_tag="ACHVB7_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1915652.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHVB7_003779"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHVB7_003779"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR1915653.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHVB7_003780"
     CDS             2474..2986
                     /locus_tag="ACHVB7_003780"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR1915654.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHVB7_003781"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHVB7_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR1915655.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHVB7_003782"
     CDS             5579..5782
                     /locus_tag="ACHVB7_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1915656.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHVB7_003783"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHVB7_003783"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR1915657.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="ACHVB7_003784"
     CDS             complement(6827..>7715)
                     /locus_tag="ACHVB7_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR1915658.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1785 a         2101 c         2020 g         1809 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg gacgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAZPJR010000189         7623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPJR010000189 DAZPJR010000000
VERSION     DAZPJR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.194"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHVB7_003785"
     CDS             complement(152..487)
                     /locus_tag="ACHVB7_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR1915659.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHVB7_003786"
     CDS             complement(491..835)
                     /locus_tag="ACHVB7_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR1915660.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHVB7_003787"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHVB7_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR1915661.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHVB7_003788"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHVB7_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR1915662.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHVB7_003789"
     CDS             1257..1376
                     /locus_tag="ACHVB7_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1915663.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHVB7_003790"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHVB7_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR1915664.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHVB7_003791"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHVB7_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR1915665.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHVB7_003792"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHVB7_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR1915666.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHVB7_003793"
     CDS             4176..4544
                     /locus_tag="ACHVB7_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1915667.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHVB7_003794"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHVB7_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1915668.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHVB7_003795"
     CDS             5295..5588
                     /locus_tag="ACHVB7_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR1915669.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHVB7_003796"
     CDS             5660..6319
                     /locus_tag="ACHVB7_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR1915670.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHVB7_003797"
     CDS             6411..6857
                     /locus_tag="ACHVB7_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR1915671.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="ACHVB7_003798"
     CDS             complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="ACHVB7_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR1915672.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1957 a         1714 c         1772 g         2180 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 cct
//
LOCUS       DAZPJR010000190         7607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPJR010000190 DAZPJR010000000
VERSION     DAZPJR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.195"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            95..1384
                     /gene="hpaG"
                     /locus_tag="ACHVB7_003799"
     CDS             95..1384
                     /gene="hpaG"
                     /locus_tag="ACHVB7_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR1915673.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1381..2847
                     /gene="hpaE"
                     /locus_tag="ACHVB7_003800"
     CDS             1381..2847
                     /gene="hpaE"
                     /locus_tag="ACHVB7_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1915674.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2849..3700
                     /gene="hpaD"
                     /locus_tag="ACHVB7_003801"
     CDS             2849..3700
                     /gene="hpaD"
                     /locus_tag="ACHVB7_003801"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR1915675.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3710..4090
                     /locus_tag="ACHVB7_003802"
     CDS             3710..4090
                     /locus_tag="ACHVB7_003802"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR1915676.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4201..5004
                     /gene="hpaH"
                     /locus_tag="ACHVB7_003803"
     CDS             4201..5004
                     /gene="hpaH"
                     /locus_tag="ACHVB7_003803"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR1915677.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5015..5803
                     /gene="hpaI"
                     /locus_tag="ACHVB7_003804"
     CDS             5015..5803
                     /gene="hpaI"
                     /locus_tag="ACHVB7_003804"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR1915678.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5978..7354
                     /gene="hpaX"
                     /locus_tag="ACHVB7_003805"
     CDS             5978..7354
                     /gene="hpaX"
                     /locus_tag="ACHVB7_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR1915679.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            7364..>7607
                     /locus_tag="ACHVB7_003806"
     CDS             7364..>7607
                     /locus_tag="ACHVB7_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR1915680.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         1748 a         2133 c         2153 g         1573 t
ORIGIN      
        1 tttacatcac ttacttttgc caaactacgc aatcgggaat aaaagcaatc attaacatat
       61 taatgattaa gatgatcgaa tccgaggagt ggtaatgaaa ggcactatct tcgccgtagc
      121 gttgaaccat cgcagccaac ttgatgcatg gcaggaagcg ttccagcaat ccccctacaa
      181 agccccgcct aaaactgcgg tctggtttat taaaccgcgc aatacggtga ttggttgcgg
      241 tgaaccgatt ccctttccac agggtgaaaa ggtactgagc ggtgcgactg ttgcgctgat
      301 tgtgggaaaa acggcgacga aagtacgtga agaagatgcg gcagagtaca tcgccggata
      361 tgcgctggct aacgacgtca gcctgccgga agagagcttt taccgcccgg caatcaaagc
      421 aaaatgtcgt gatggattct gccccattgg cgaaaccgtg gctctcagca atgtcgataa
      481 tctgaccatc tataccgaga tcaacgggcg tcctgccgat cactggaata ccgccgattt
      541 acaacgtaac gccgcgcagt tgctgagcgc cctgagcgaa tttgccacgc tgaatccagg
      601 cgacgccatt ctgctcggca cgccacaggc gcgcgtggaa atacagccag gcgatcgcgt
      661 tcgtgttctc gcagaaggtt tcccgccgct ggaaaatccg gtagtggacg aacgtgaagt
      721 gaccacgcac aagagcttcc caacgcagcc acacccgcac ggcacgctgt ttgccctcgg
      781 cctgaactac gccgaccacg ccagcgaact ggaatttaag ccaccggaag aaccgctggt
      841 gttcctgaaa gcgccaaata ccctcactgg cgataaccag acctccgtgc gtccaaacaa
      901 tattgaatac atgcactatg aagcggagct ggtggtagtt attggcaaac aggcgcgtaa
      961 cgtcagcgaa gccgatgcca tggattatgt cgcgggctac accgtgtgta acgactacgc
     1021 cattcgtgac tatctggaaa actactaccg ccctaacctg cgggtaaaaa gccgcgacgg
     1081 actgacgccg atgctttcaa ccatcgtgcc gaaagaggcg atcccggacc cgcataatct
     1141 gacccttcgc accttcgtca acggcgagtt acgccagcaa ggcaccaccg ccgatctgat
     1201 cttcagcgtg cccttcctga tcgcctactt aagtgaattt atgaccctga atccgggcga
     1261 catgatcgcc accggcacac ccaaaggctt atctgacgtg gtgcctggcg atgaagtagt
     1321 ggtggaagta gaaggcgtgg gccgcctggt gaaccgaatt gtgagtgagg atacagcgaa
     1381 atgaaaaaag taaatcattg gatcaacggc aaaaatgttg caggtaacga ctacttccag
     1441 accaccaatc cggcaacggg tgaagtgctg gcggatgtgg cctctggcgg tgaagcggag
     1501 atcaatcagg cggtagcggc agcgaaagag gcgttcccga aatgggccaa tctgccgatg
     1561 aaagagcgtg cgcgcctgat gcgccgtctg ggcgatctga tcgaccagaa cgtgccagag
     1621 atcgccgcga tggaaaccgc ggacaccggc ctgccgatcc atcagaccaa aaatgtgttg
     1681 atcccacgcg cttcccacaa ctttgaattt ttcgcggaag tctgccagca gatgaacggc
     1741 aagacctatc cggttgacga caagatgctc aactacacgc tggtgcagcc ggtgggcgtt
     1801 tgtgcgctgg tatcgccgtg gaacgtaccg tttatgaccg ccacatggaa ggtcgcgccg
     1861 tgtctggcgc tgggcaatac cgcggtactg aaaatgtcgg aactctcccc gctgaccgct
     1921 gacagcctgg gtgagctggc gctggaagcc gatattccgg caggcgtgct gaacgtggta
     1981 cagggctacg gcgcaaccgc aggggatgcg ctggttcgtc atcatgacgt acgtgccgtg
     2041 tcgttcaccg gcggtacggc caccgggcgc aacatcatga aaaacgccgg gctgaaaaaa
     2101 tactccatgg aactgggcgg taaatcgccg gtgctgattt ttgaagatgc cgatattgaa
     2161 cgcgcgctgg acgccgccct gttcaccatc ttctcgatca acggcgaacg ctgcaccgcc
     2221 ggttcgcgca tctttattca gcaaagcatc tacccggaat tcgttaaacg ctttgccgaa
     2281 cgcgccaacc gtctgcgcgt gggcgatccg aacgatccga atacccaggt tggcgcgctt
     2341 attagccagc agcactggga aaaagtctcc ggctatatcc gtctcggcat tgaagaaggc
     2401 gcaaccctgc tggcgggcgg cccggataaa ccgtccgacc tgcctgcgca cctgaaaggc
     2461 ggcaacttcc tgcgcccaac cgtgctggca gacgttgata accgtatgcg agtcgcccag
     2521 gaagagattt tcgggccggt cgcctgcctg ctgccgttta aagacgaagc cgaaggctta
     2581 cgtctggcaa acgacgtgga gtacggcctc gcgtcgtaca tctggacaca ggatgtcagc
     2641 aaagtgttac gcctggcgcg tggcattgaa gctggcatgg tgttcgtcaa cacccagaac
     2701 gtgcgtgacc tgcgccagcc atttggcggc gtaaaagcct ccggcaccgg gcgtgaaggc
     2761 ggtgagtaca gcttcgaagt gttcgcggaa atgaagaacg tctgcatttc catgggcgac
     2821 catccaattc cgaaatgggg agtctgatat gggtaagtta gcgttagccg ccaagatcac
     2881 gcacgtaccg tcgatgtatc actctgaact gccagggaaa aaccacggtt gccgccaggg
     2941 cgcgatcgac gggcataaag agatcagcaa gcgttgccgg gaaatgggcg tcgataccat
     3001 tatcgttttc gatacccact ggctggtcaa cagtgcttat cacatcaact gtgcagacca
     3061 ttttgaaggc gtctacacca gcaacgagct gccgcatttt atccgcgata tgacctacaa
     3121 ctacgagggc aacccggagt tggggcagct tattgccgat gaagccttaa agctcggcgt
     3181 gcgggcaaaa gcgcacaaca ttcccagcct gaaactggaa tacggcacgc tggtgccgat
     3241 gcgctacatg aatgaagaca agcacttcaa agtggtctcc atttcggctt tctgcacggt
     3301 tcacgatttt gccgacagcc gcaagctggg cgaagcgatt ctgaaagcga tcgaacagta
     3361 cgacggcacc gtggcggtcc ttgccagcgg ttcgttatcg caccgcttta ttgacgatca
     3421 gcgtgcggaa gaagggatga acagctacac ccgcgagttc gaccgccaga tggacgagcg
     3481 cgtggtgaag ttgtggcgcg aaggccagtt caaagagttc tgcaatatgc tgccggagta
     3541 cgccgactac tgctacggcg aaggcaatat gcacgacacg gtgatgctgc tggggatgct
     3601 cggctgggat aaatacgacg gcaaggtgga gtttatcacc gagctattcc caagctctgg
     3661 caccggtcag gttaacgctg ttttcccgct tcccgcgtaa ggacgtttta tgccgcactt
     3721 tatcgttaaa tgcagtgaca acatccgcga agaagccgac ctgccggggt tgttcgccaa
     3781 agtgaatccg acgctggcag ccacgggtat ttttccgctg gcgggtattc gcagccgcgt
     3841 gcattgggtc gatacctggc agatggccga cgggcagcgt gattatgcct tcgtgcatat
     3901 gacgttgaaa atcggcgcag gtcgcagcct ggaaagccgc cagcaggcgg gtgaaatgct
     3961 gtttgaactg attaaaacgc acttcgccgc cctgatggag agccgcctgc tggcgttgtc
     4021 gtttgagatt gaagagctgc atccgacgct gaattttaaa caaaacaacg tgcacgcatt
     4081 gtttaagtag cgcgcagatt gcccggtggc gctgcgctta ccgggcctac aaaaccccaa
     4141 accgtacacc gtaggccgga taaggcgcag ccgcatccgg caatgccaca ggatatcgct
     4201 atgttcgata aacacaccca caccctgatc gcccagcgtc tggatcaggc agaaaaacag
     4261 cgcgaacaga tccgcgcgat ctcgctggat tacccggaga tcaccatcga agacgcttac
     4321 gcggtgcagc gtgaatgggt tcgcctgaaa atcgccgaag gtcgcacgct gaaaggccac
     4381 aaaatcggcc tgacctcgaa agcgatgcag gcaagctcgc agatcagcga accggattac
     4441 ggcgcgctgc tggatgacat gttcttccac gatggcagcg atatcccgac cgatcgcttt
     4501 atcgtgccgc gcattgaagt ggagctggct ttcgtgctgg caaaaccgct gcgtgggcca
     4561 aactgcacgc tgttcgacgt ctacaacgcc acggactacg tgatcccggc gctggagctg
     4621 atcgacgctc gctgccacaa catcgatccg gaaacccagc gcccgcgcaa agtgttcgac
     4681 accatttctg ataacgccgc caacgccggg gtgatcctcg gcggtcgtcc gatcaaacca
     4741 gacgaactgg atctgcgttg gatctccgcc ctgatgtatc gcaatggcgt gattgaagaa
     4801 accggcgtcg ccgctggcgt gctgaatcat ccggcaaacg gcgtggcctg gctggcgaac
     4861 aaactcgcac cgtatgacgt acaactggaa gccgggcaaa tcattctcgg tggttcgttc
     4921 acccgcccgg ttccggcgcg taagggcgac accttccacg tcgattacgg caacatgggt
     4981 tccattagct gccgctttgt ttaaggagag aacgatggaa aacagcttta aagcggcgct
     5041 gaaagcaggc cgtccgcaga tcggattatg gctggggctg agtagcagct acagcgcaga
     5101 gttactggcc ggagcaggat tcgactggtt gttgatcgac ggtgagcaca cgccgaataa
     5161 cgtgcaaacc gtgctcaccc agctacaggc gattgcgccc taccccagcc agccggtagt
     5221 acgtccgtcg tggaacgatc cggtgcaaat caaacaactg ctggacgtcg gcacacaaac
     5281 cttgctggtg ccgatggtac aaaacgctga cgaagcccgt gaagcggtac gcgccacccg
     5341 ttatcccccc gccggtattc gcggtgtggg cagtgcgctg gcccgcgcct cgcgctggaa
     5401 tcgtattcct gattacctgc aaaaagccaa cgatcaaatg tgcgtgctgg tgcagatcga
     5461 aacgcgtgag gcaatgaaga acttaccgca gattctggac gtggaaggcg tcgacggcgt
     5521 gtttatcggc ccggcggatc tgagcgccga tatgggttat gccggtaatc cgcagcaccc
     5581 ggaagtacag gccgccattg agcaggcgat cgtgcagatc cgcgaagcgg gcaaagcgcc
     5641 ggggatcctg atcgccaatg agcaactggc aaaacgctat ctggaactgg gcgcgctgtt
     5701 tgtcgccgtc ggcgttgaca ccaccctgct cgcccgcgcc gccgaagcgc tggcagcacg
     5761 gtttggtgcg caggccaccg ccgtgaagcc cggcgtgtat taatgccgga tgcgctgcgc
     5821 ttatccggcc tacactcgca ccaaaccgta ggccggataa ggcgtttacg ccgcatccgg
     5881 caaaaagccg taccaaatcg cggaccggat aaggcgttac gccgcatccg ccataaacct
     5941 tgtcgtaccc tacaaaaatc ccattagagg aagaaaaatg agcgacacct cacctgccat
     6001 accggagagt atcgatccgg cgaatcagca taaagcgctg actgccggac aacaggcggt
     6061 tattaagaag ctatttcgcc gcctgagcgt ctttctgttc gtgctgttta tcttctcgtt
     6121 ccttgatcgc atcaacatcg gctttgccgg actcacgatg ggacgcgacc tcggtctgag
     6181 cgccaccatg tttggcctcg ctaccaccct gttctatgcc gcttatgtca tcttcggcat
     6241 tcccagcaac attatgctga gtattgtcgg tgcgcggcgc tggatcgcca ccatcatggt
     6301 gctctggggc atcgcctcta ctgccaccat gtttgccact ggccccacca gcttgtacgt
     6361 actgcgtata ctggttggca ttaccgaagc cggctttctg cctggtattc tgctgtattt
     6421 aaccttctgg tttccggcct atttccgcgc ccgtgccaac gccttgttta tggtagcaat
     6481 gccggtaacg acagcgttgg gctcgatcgt ttccggctac attttgtcgc tggatggcgt
     6541 catggcatta aaaggctggc agtggctgtt tttgctggaa ggcttcccgt cggtattact
     6601 cggcgtcatg gtgtggttct ggcttgatga ctcaccggac aaagctaagt ggctgacgaa
     6661 agaagacaaa aaatgcctgc aagagatgat ggataacgat cgtctgacgc tggttcagcc
     6721 agagggagcc atcagccacc acgccatgca acaacgcagt atgtggcggg agatcttcac
     6781 tccggtggtg atgatgtata ccctggcgta tttctgcctg accaacacac ttagtgcgat
     6841 cagcatctgg acaccgcaga tcctgcaaag ttttaatcag ggcagcagta atatcaccat
     6901 cggcatgctg gccgccgtac cgcagatttg taccattctc gggatggtct actggagccg
     6961 tcactcagat cgccgccagg aacgaaggca tcacaccgcc cttccttatt tgttcgctgc
     7021 cgcaggttgg ttactggctt cggcaactga tcacaacatg atccagatgc tggggatcat
     7081 tatggcttcg accggatcat tcagcgcaat ggcgattttc tggacaacac cggatcagtc
     7141 catcagcctg cgggcacgag cgatcggtat tgcggtgatc aacgccactg gcaacattgg
     7201 ttcagcgtta agtccgttta tgatcggctg gttgaaagat ttgaccggca gctttaacag
     7261 tggattgtgg tttgttgccg cgctgctggt gattggtgcg gggattatct gggcgattcc
     7321 aatgcagtcc tcccgtccgc gagcgaccct gtaaggaacg acgatgtgtg accgtcagat
     7381 tgccaatatt gatatcagca aagagtacga tgaaagcctg ggcacggacg atgtgcatta
     7441 tcagtcgttc gcccgcatgg cggcattttt tggccgccat atgctgccac atcgccacga
     7501 acagtacttt cagatgcatt tcctcaatag cggacagatt gagctacagc ttgacgatca
     7561 tcgctactcg gtggaagcgc ccctgtttgt cctgacgccg ccgtcag
//
LOCUS       DAZPJR010000191         7594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPJR010000191 DAZPJR010000000
VERSION     DAZPJR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.196"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..93
                     /locus_tag="ACHVB7_003807"
     CDS             <1..93
                     /locus_tag="ACHVB7_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915681.1"
                     /translation="KYNAYEAYNSENLYFTREEYTYDYDLLNAI"
     gene            complement(707..1083)
                     /gene="rnpB"
                     /locus_tag="ACHVB7_003808"
     ncRNA           complement(707..1083)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHVB7_003808"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1116..2261)
                     /gene="garK"
                     /locus_tag="ACHVB7_003809"
     CDS             complement(1116..2261)
                     /gene="garK"
                     /locus_tag="ACHVB7_003809"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR1915682.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2358..3248)
                     /gene="garR"
                     /locus_tag="ACHVB7_003810"
     CDS             complement(2358..3248)
                     /gene="garR"
                     /locus_tag="ACHVB7_003810"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR1915683.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3278..4048)
                     /gene="garL"
                     /locus_tag="ACHVB7_003811"
     CDS             complement(3278..4048)
                     /gene="garL"
                     /locus_tag="ACHVB7_003811"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR1915684.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4064..5398)
                     /gene="garP"
                     /locus_tag="ACHVB7_003812"
     CDS             complement(4064..5398)
                     /gene="garP"
                     /locus_tag="ACHVB7_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR1915685.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5773..7344
                     /gene="garD"
                     /locus_tag="ACHVB7_003813"
     CDS             5773..7344
                     /gene="garD"
                     /locus_tag="ACHVB7_003813"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR1915686.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7493..>7594
                     /gene="prlF"
                     /locus_tag="ACHVB7_003814"
     CDS             7493..>7594
                     /gene="prlF"
                     /locus_tag="ACHVB7_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFR1915687.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALK"
BASE COUNT         1904 a         2056 c         1834 g         1800 t
ORIGIN      
        1 aaatataacg catatgaagc atataactca gagaatctat attttaccag agaggaatat
       61 acgtatgatt acgacctttt aaatgcaata tagacataat aacttcttac aaaatctata
      121 attaataccg tagatattca ttcatcttac cctgtgcacc aaaagcaaaa actcactgac
      181 gcgggtttga aactgatggc aaccgcaaaa atgcctgatg cgctacgctt atcaggccta
      241 cgccatctct acaatatatt gaatttgcgt gcttttgtag gcaggataag gcgttcacgc
      301 agcatccggc atgaataaag cgcactcaac aatctgaaac ccgccggaac ggtttattac
      361 gtacatcagg tgaaactgac cgataagccg ctttcttttg ggtatagtgt cgtggacagt
      421 cattcatctt tctgcccctc caaaagcaaa aacccgccga agcgggtttt tacgtaaatc
      481 aggtgaaact gaccgataag ccgctttctt ttgggtatag cgtcgtggac agtcattcat
      541 ctttctgccc ctccaaaagc aaaaacccgc cgaagcggtt ttttacgtaa accaggtgaa
      601 actgaccgat aagccgcttt cttttgggta tagcgtcgtg gacagtcatt catctttccg
      661 ctcctccaaa agcaaaaacc cgccgaagcg ggtttttacg taaatcaggt gaaactgacc
      721 gataagccgg gttctgtcgt ggacagtcat tcatctaggc cagcaatcgc tcactggctc
      781 aagcagccta cccgggttca gtacgggccg taccttatga acccctattt ggccttgctc
      841 cgggtggagt ttaccgtgcc acggactgtt accagccgcg cggtgcgctc ttaccgcacc
      901 ctttcaccct tacctgatcc cgcttgcgcg ggccatcggc ggtttgctct ctgttgcact
      961 ggtcgtgggt ttccccccca ggcgttacct ggcaccctgc cctatggagc ccggactttc
     1021 ctcccctccg cccgtctccc ccgaagagga cgacgacgaa gcggcgactg tctggtcagc
     1081 ttcggcgcgc agtatagagg gtttgcgcgc ccttgtcacc ccgcgttgcg cattccaatc
     1141 gccagtgtcg cggcgatatt acgtgaagca cggcagatat tgtcataagc cccgcggaat
     1201 gcttcgtcca acgtacctat gctggtcaat acgctgaaga ccgcatcgat gccatgctga
     1261 tgtacaacgc caacatcatc ggtcaggcta cccgcaatgc caatcaccgg tttatggtac
     1321 ttcttcgcca cgtttgcaac gccaatcggt accttgccgt gaatactctg gctgtcaata
     1381 cgcccttcac cggtgatcac cagcgtacaa tcgtgaatat gttcttccag attcagcgcc
     1441 gtagtgacga tttcaatacc acttttcagt tccgcaccaa gaaacgccat cagcgccgcg
     1501 cccataccgc ccgcagctcc tgcgccgggg acatctttca catcaacatg cagcgctttt
     1561 ttaatgacat cggcatagtg agagaggtta ttgtccagct caacaatcat cgcttcactg
     1621 gctccctttt gtgggccaaa gatgcgcgat gcgccgttat cgcccaccag cggattggtg
     1681 acatcacaag cgacgcgaat gacgcaatct tttaagcgcg gatcgaggcc ggaaatatca
     1741 atatcattca gagtattaag actaccgccg ccaaaaccaa tttcattgcc gttggcgtcg
     1801 cataatttcg cccccagcgc ctgtaccatg cctgcgccgc catcatttgt agcgctgccg
     1861 ccaatgccga taataatgtt tgttgcactg ctctccagcg cctgcaggat taactcgcct
     1921 gtgccgcgtg aagtggttac gagtggatcg cgtttttccg caggtaccag ctccagccca
     1981 ctggccgccg ccatttcaat aaacgcggtt ttgccatcgc cggagatccc ccaactggca
     2041 ttcactttct cgcccagcgg ccctgtaacc caggcgtgac gttcagcccc ctgggtggct
     2101 gcaatcatcg cttccaccgt tccttcacca ccgtcggcaa ccggaacaga aacatactgt
     2161 gcatcaggaa aaatttcccg aaatcctttt tctatcgcct gcgcaacctc gctggcagat
     2221 aaactttctt tataagagtc tggggcgatt acgattttca tacctatgcc tgttaccaca
     2281 tgacgccgga gggcgtttct cttattcggc ctagattcca ggcccggatt gcaatacacc
     2341 atccgggcac gacgtcatta acgagtaact tcgactttcg ccagtttttc gtagtagcac
     2401 gccagggcgc tatgatccgc cgttcctaaa ccatctgcac gcagtgcctg catcatctcc
     2461 ataaccgcag ctgtgagcag cagttgtgcg ccgacgccgt gagaagtatc cagcgcattc
     2521 gccagatcct taatatgcag atcaatgcgg aagcccggct tgaagttgcg gtccatcacc
     2581 atcggcgctt tggcatccag cacggtactg cccgccagcc caccgcgaat tgcctgataa
     2641 accaggtccg ggttaacgcc cgctttggtt gccagcgtta acgcttctga catcgcagca
     2701 atattcagcg ccacaatgac ctgatttgcc agtttggtga cgttacctgc accgatttcc
     2761 ccggtatgca ccacggaacc cgccatcgct ttcatcaaat catagtattt gtcgaaaata
     2821 gccttgtcgc cgcccaccat cactgacagc gtaccgtcga tggctttcgg ttcaccgccg
     2881 ctcaccggag catccagcat atcaatgcct ttcgctttca gcgcttcgct gatttcacgg
     2941 cttgccagcg gtgcgataga actcatatcg atcaataccg tacctggctt cgcgccttca
     3001 ataatgccat tctcacccag cgccacctct ttcacatgag gggagtttgg cagcatggtt
     3061 atgatgacgt cgcactgttc agcgatcgct ttagccgtag acgctgtttc tgcacctgca
     3121 gcaatcacgt cagcaatagc ttctgggtta cggtcagcaa ccaccagcga gtaacctgct
     3181 ttcagaaggt ttttactcat tggtttaccc ataatcccca ggccaataaa accaactttc
     3241 atagtcatat caaccatctc tcttgttgcg gtggtggtta ttttttaaag gtatcagcca
     3301 gtttctgagt ggcagagcgg aagacgccaa gatcgctgcc gacagccaca aacgtcgcgc
     3361 cccattccag ataacgacgc gcatcggctt cgaccggcgc gagaataccg ctgggtttgc
     3421 cgtgcgcgct ggcacggtta aaaatatgct gaattgcttt ttgtacatcc ggatgcgagg
     3481 cattgccgag atggcccaat gccgcagcca gatcgctggg gccgacgaag ataccgtcta
     3541 caccttcggt agcggcaata gcatcgacgt tatctacgcc ctgctgactt tctatctgga
     3601 ccagaatagt aatgttcttg ttcgactgag cgaaataatc cgccacggtg ccaaacatat
     3661 tggtgcggtg agaaacggag acgccgcgaa tgccttccgg cgggtaacga gttgatgcca
     3721 ccgcctgctc tgcttcctct tttgtttcta caaaaggaat caggaagtta tagaaaccga
     3781 tatccagaag acgcttaata attaccggct cgttggtcgg cactcgcact actggcgcgc
     3841 tggcgctgcc tttcagggcc attaactgcg gaataaacgt ggaaatatcg tttggcgcat
     3901 gttcgccatc cagcaccagc cagtcaaacc cagccaaacc aagaacttca gtgctaatcg
     3961 ggttagagag tgctgaccag caaccaattt gtacctgttt cgcagccagt gcggctttga
     4021 atttattcgg gaaaacatcg ttattcatcg cttatacctt tgcttatttc tgcaattcca
     4081 tacgtttaat gtcgccaact acgaagaggt agcagaccat cgccatcagc gctgaacatc
     4141 ccacgaaaac cagtgctgca ttgaaggagt gcagttcact taccaggtag ccaatcacca
     4201 gtggagtgac aatggaggca acattgccaa agacgttaaa gacgccgcca cagaggccaa
     4261 caatctcttt cggcgcggtg tcagaaatca ccggccagcc cagagcacca aatcctttgc
     4321 caaaaaaagc cagcgccatc agcatgacca ccagcgtggt gttgttggtg tagttacata
     4381 agatgatggt ggaaaccagc aacattccca gcacaatcgg tagcttacgt gccagggtca
     4441 gggataaacc gcgtttgatc agataatccg agaagacacc tcccagcacg ccgcccgcaa
     4501 aaccacacag tgccggaatc gaggcgacca gacccacttt cagaatcgac atgccttttt
     4561 cctgcaccag ataaatcggg aaccaggtga ggaagaacca ggtgatggtg ttgataaaat
     4621 attgtccgaa aaatacgccc aacatcatgc ggttagagag caattgcttg atgtaatgca
     4681 gtttgggacc gcttgctgcc gcgctgcccg gctttttgtg gtccatatcg accaccgcgc
     4741 cattttcaga gataaacttc agctcttccg cagacatacg tgggtgatct gtcgggttat
     4801 gaatcaactt gatccacagc gccgtcagca caaaaccaat cccccccata acggtgaaga
     4861 cgtgctccca gccccaagcg aaagtcagcc agccaagcag cggcgaaaag agcgccagcg
     4921 agaaatattg cgccgagtta aagatggcgg aggcagtacc acgttctttc gtcgggaacc
     4981 aggcggcgac aatacgggcg ttcgccggga atgatggcgc ttccgagaac cccagcataa
     5041 agcgcataaa gaacatggag atccctgccc aggccagcgg gaacatatca acaaagcctt
     5101 gcaggaaggt gaacagcgac cagaaaaaga ggctgtaggt gtaaactttt ttcgagccaa
     5161 acttatcaag caaccagccg ccggggattt gcatcagcaa gtaggcccag ccaaaagcag
     5221 agaagatgta acccatcgaa accgcactta actgcaactc ttttgccact tcggtaccag
     5281 caatagacag cgttgcacga tcggcgtagt taacggcggt aacaataaaa ataatcagta
     5341 atattaaata gctggtatgc acgcctttct tttttacgtc aacggtgtcc agaatcattt
     5401 tatttacctc gggtacttat gctgattttt attattatgg ggaaggtgtt atttatgagt
     5461 tttatttatg ccgttacgac aatgaactcg ggaattagta taagcagcgg gagaataata
     5521 atcattgtgc aaatgctaat ttaatcagta ctatttaaat attattttga gcatatgcac
     5581 ataaggttgc gcgctaaagc acagatttgc gctttacctt accgggcggc actgcaatcc
     5641 ctgaaatgat tgacattgat cacatttctg cgtttaaact cctgacattc ttatttcacc
     5701 caatgaagtc atttattttt aaatgagacc agatcctcat tttaataacc cctggctgga
     5761 gaatattgca caatggccaa catcgaaatc agacaagaaa cgccaactgc gttttatata
     5821 aaagttcacg acacagataa tgtggcaatt attgttaatg ataatggcct gaaagcagga
     5881 acgcgttttc cggatgggct ggaattaatt gaacatactc cccaggggca taaagtcgca
     5941 ttgctggaca ttccagctaa tggtgaaatt attcgttatg gcgaagtgat tggttacgcc
     6001 gtgcgcgcaa tcccacgcgg aagctggatc gacgaatcaa tggttgtact accggaagcg
     6061 ccgccgttac acacgctgcc actggcaacc aaagtcccgg cccccttacc gccgctggaa
     6121 ggatacacct ttgagggcta tcgcaatgcc gatggcagcg tgggcaccaa aaacctgctc
     6181 ggcatcacca ccagcgtcca ctgtgtggca ggcgtggtgg actatgtagt aaaaatcatt
     6241 gaacgcgatc tgctaccgaa atacccgaac gtcgatggcg tggtggggct gaatcatttg
     6301 tacggctgtg gcgtggcgat taacgcaccg gcggcagttg tgcctattcg taccatccac
     6361 aatatttcgc tgaaccctaa ctttggcggc gaagtaatgg tgattggtct gggttgtgaa
     6421 aagttgcagc ctgagcgcct gctggtcggc acggatgatg tgcaagctat tccagtagaa
     6481 agcgccagca ttgtcagttt gcaggatgaa aagcatgtcg gctttcagtc catggtcgag
     6541 gatattttgc aggtcgccga acgccatcta caaaaactga atcaacggca gcgagaaacc
     6601 tgcccggctt cagaactggt cgttggtgtg cagtgcggtg gcagcgatgc gttttctggt
     6661 gtaacggcaa acccggcggt tggctatgcg tctgatctac tggtgcgctg cggcgcaacg
     6721 gtgatgtttt cagaagtaac ggaagtgcgt gacgcgatcc atctgctgac accacgcgca
     6781 gtgaacgaag aggtcggcaa acggctgctg gaggagatgg agtggtacga taactatctc
     6841 aatatgggaa aaaccgaccg cagcgccaac ccttcgccgg gcaacaagaa aggcggtctg
     6901 gcaaatgtgg tggagaaggc actcggctcc attgctaaat cgggtaaaag tgcaattgtt
     6961 gaagtgctgt cgcccggtca acgcccgact aaacgcggat taatttacgc cgcgacgcca
     7021 gccagcgatt ttgtctgtgg cacgcaacag gtggcttcgg gtatcaccgt acaagtgttt
     7081 acgaccggcc gtggtacgcc gtacggcctg atggcggtac ccgtaattaa aatggcaacc
     7141 cgcaccgagc tggcgaaccg ctggtttgat ttaatggata ttaacgcggg cactatcgct
     7201 accggcgaag agagcattga agaggtgggc tggaagttgt tccactttat tctcgacgtc
     7261 gccagcggga agaagaaaac cttctcggat caatgggggc tgcataacca gctggcggtg
     7321 tttaacccgg caccggtgac ctgatttctt ttggaacgtc cctcgcaaaa catggcctta
     7381 gtgccatgtt tttattgttt aaagccccca cgtccattaa taatgcattt gcattacctt
     7441 agttcaagct tataatttga gcagaaaaca ggctgtaaaa ggacaatgaa tcatgcccgc
     7501 taatgctcgc tctcacgctg tactgaccac tgaatcaaag gtcacgatac gcggacaaac
     7561 aactatcccc gcgccagtgc gtgaggcctt aaaa
//
LOCUS       DAZPJR010000192         7565 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPJR010000192 DAZPJR010000000
VERSION     DAZPJR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.197"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(272..751)
                     /gene="folK"
                     /locus_tag="ACHVB7_003815"
     CDS             complement(272..751)
                     /gene="folK"
                     /locus_tag="ACHVB7_003815"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR1915688.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(748..2145)
                     /gene="pcnB"
                     /locus_tag="ACHVB7_003816"
     CDS             complement(748..2145)
                     /gene="pcnB"
                     /locus_tag="ACHVB7_003816"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR1915689.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2205..3101)
                     /gene="gluQRS"
                     /locus_tag="ACHVB7_003817"
     CDS             complement(2205..3101)
                     /gene="gluQRS"
                     /locus_tag="ACHVB7_003817"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR1915690.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(3168..3623)
                     /gene="dksA"
                     /locus_tag="ACHVB7_003818"
     CDS             complement(3168..3623)
                     /gene="dksA"
                     /locus_tag="ACHVB7_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR1915691.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3801..4505)
                     /gene="sfsA"
                     /locus_tag="ACHVB7_003819"
     CDS             complement(3801..4505)
                     /gene="sfsA"
                     /locus_tag="ACHVB7_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR1915692.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4520..5050)
                     /gene="thpR"
                     /locus_tag="ACHVB7_003820"
     CDS             complement(4520..5050)
                     /gene="thpR"
                     /locus_tag="ACHVB7_003820"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR1915693.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5124..7553
                     /gene="hrpB"
                     /locus_tag="ACHVB7_003821"
     CDS             5124..7553
                     /gene="hrpB"
                     /locus_tag="ACHVB7_003821"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR1915694.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1759 a         1928 c         2086 g         1792 t
ORIGIN      
        1 aatgatattt gttaccaaaa tttacaacca ttgttcatta ggacgcctat tgtgcacttt
       61 aagggctttt gaacaaatta aattttctta attcaaaatt aagcaaaaat aaattcacaa
      121 gtgcaattgg ttagggtatg gcgatgggat tgatatttat ctgcgggaat ttatattatg
      181 tttctgcaaa aatgtttaaa ctaaaatttt tattaaggaa agagaggatg tatgattttc
      241 agaaaatatt tatattcgta atataaataa attaccattt gcttaattcg tcaaatgctc
      301 ttgtatgtaa gacttcacgc aacgtctccc catcaggaaa cgccagctcc ggtgcaatct
      361 caaacagcgg ccacagcata aatccacgat tcttcatatc gtagtgcgga acggtcaggc
      421 gttcggtatt tatcacttca ttaccaaaca gcatgatatc gagatccagc gtgcgtggtc
      481 cccagcgttc agctttacga acacgaccct gttgcagttc tatgcgctgt gtatggttga
      541 gtaactcttc cggggcgagc gaagtttcaa gcgccacggc ggcgttgagg taatcgggtt
      601 gatcttgcgg ccccagcggt ggagtgcggt aaaacgaaga aacggcaaga atgtggcttt
      661 cagggatatc gcctaatgct ttcagggcag cattgacctg ctccagcgga gaggccagat
      721 tgctgcctat ggcaatatac gccactgtca tgcggtaccc tcacgacgcg gtgcgcgttt
      781 gcgtggacga cgagtacggc gacgcggtga cggctcttca tccaactcgt taagcatccc
      841 tttttgatct ggtggcgcgg aaacctggaa ctcaccccac catttcacca gacgctgcag
      901 ttcagcgtta cgttcaactt cagctcgcaa ggccaacagg tcataagccg cacggaactt
      961 aggatgctcc agcagtttcc atgcgcgttt accctgacgt cgggacatac gcaactgcaa
     1021 ctgccagata tcgcgggtta acgtcgtcag gcgtttcggg attgccagtg aacggcaggc
     1081 ttcgtccagc acgtcgttca tcgccagcgc gaaagcgtcg tgataggtca ggccgctttc
     1141 ctgggcgatc ttctgtgccg tctccagcag tgggtaccag aacatggcgg caaacaagaa
     1201 cgccgggttc acgcgcatat cgttatggat acgcgtatcg gtattcttca gcacctgttc
     1261 aatgatccgc tccatcgggc tgtcgccatt ttccgtgaag tagcgggtaa tggtcgggaa
     1321 cagcggctgg aacagatgat attcacacaa cagcttatag gtttcgtaac cgtagcccgc
     1381 ttgtagcagt ttaagcgatt cttcaaacag gcgtgccggt gggatatcgt tcagcagggt
     1441 agcgaggcga gggatcggtt ctgcggtttc cgggctgatg cgcataccca atttggcggc
     1501 aaaacgtacc gcgcgcagca tccgtaccgg atcttcacgg tagcgcgttt ccgggttacc
     1561 aatcagacgg ataacgccgt ccttcagatc cttcataccg ccaacgtaat cacggacggt
     1621 aaaatccgct acgctgtaat agaggctgtt gatagtgaaa tcgcggcgct gggcgtcttc
     1681 ttcgatggag ccgaaaatgt tgtcgcgcag caacatgccg ttttgcccgc gttgggaggt
     1741 cgtgcggtcg ctgacgttac cttcgtggtg tccacggaag gtcgcaactt cgataatctc
     1801 cgggccaaac attacatgag ccagacggaa acggcgaccc accaggcggc agttacggaa
     1861 cagtttgcgc acctgctcag gcgtggcgtt agtggttacg tcaaaatctt tcggcttttt
     1921 gccaagtaac aggtcgcgca cgccgccgcc aaccagccag gcttcgtatc ccgctttgtt
     1981 gagcctgtac attaccttca gggcattttc actgatatct ttgcgggaaa tagcatgctg
     2041 ctcacgcggg atcaccgtca cctgtggacg ggcgactgcc tgttcagcct cgctttcctc
     2101 gcggcttagc accttgcggc aaaaattagc gactcgggta aaaatagtac acctcggtag
     2161 tgtcaaacat cattcaggac aaaaaaatag cggctaatca tagctcagca tgacgcattt
     2221 gagaatgttg aatttacaat tgccgactcg ggcacggcgg tcagcgtcca gtttttgact
     2281 gctgactgaa ggatttgttc gacgctgaaa tcctgccagt gtgcttctgc ctgctgaccc
     2341 agaaattgaa gtgccgcgat tagtaccgga cgtggatcgc ctttcggcaa cgcaggcgca
     2401 tgattctgct tggaaagttt agcgccttgt ggattaagcg ccagcggcag atgaatgtaa
     2461 tctggcactt tccagccaaa aagctggtac agcgagattt gccttactgt tggttcaatc
     2521 agatcagccc cacgcactat ttctgtaacg ccctggaaat gatcatcaac cacaacagcc
     2581 aggttgtagg cgaacaaccc atcacggcga tgaatgataa aatcttcccg tgccagtttt
     2641 tcgtcggcgt gaataatgcc gcgcagctgg tcagtaaatt gcgtgaccgg atgctgctgg
     2701 cggatacgca ctgcggcatt gtctggtcca tgatgcaaca cccggcaatg accgtcgtaa
     2761 ataccgccaa tgctttgaat acgcgcacgc gtacaggtgc agtaataact tagtccttgt
     2821 tcatgtaacc aggcgagtgc ttcacgatag gcgtcgtgac gttgcgattg ccagagaaca
     2881 tcgccgtccc agtgcagacc gtaatgttcc agctggcgca ggatagtttc tgcggcaccg
     2941 ggaacttcac gaggcgggtc gatatcttct atgcgtacca gccagcgacc ttgccgggcg
     3001 cgagcctgca aatagctgcc gagcgcggcg atcagagagc caaaatgaag ctcgccggaa
     3061 ggagaggggg cgaagcggcc aatatactgt gtgtctgtca tctctttgaa caaaaaataa
     3121 ggcgggagca tttcccgcct gtggtaaacg tgatggaacg gctgtaatta gccagccatc
     3181 tgtttttcgc gaatttcagc cagcgttttg cagtcgatgc acagatcggc tgtcgggcgc
     3241 gcttccagac ggcgaatacc aatttcaaca ccgcaggatt cgcagtagcc gaaatcttcg
     3301 tcttccactt ttttcagcgt cttctcgatc tttttgatca gcttacgctc gcgatcgcgg
     3361 ttacgcagtt cgaggctgaa ctcttcttcc tgggctgcac ggtctaccgg gtccgggaag
     3421 ttggctgctt catcctgcat atgtgtaacg gtgcgatcga cttcatccct gagttgatta
     3481 cgccatgctt ccagaatacg acggaagtgc gccagctggg cttcattcat atactcttcg
     3541 cccggcttct cctgatatgg ttccacccca gcgatggcga gaatactcag ggacgatgtt
     3601 ttacggtttt gcccttcttg catgttgctt ctccttaaca cgcactatcg atccccatgt
     3661 tcgggggaaa aatgaggccg ctataaatag cagatgcttt tccggatagc aattatctaa
     3721 acgtaacact tgacaactgt gtgaggaaaa gcgtatttgc gcacgcgacc agaatgtaaa
     3781 ttaaccagtt acttacttta ctacaatgta accgacagtg attttttaag agccatgcct
     3841 tcagcagaaa tttccgcttt gtaagccaga atttctaccc ccctctgttg agcttctgac
     3901 aatagttgcg cgtatttctc atcgatgtgg cgcgcgggtg aaaaccgtgt aatggctgaa
     3961 tgcagcacgg cgaaaaagat aaccgcacgc tggccttcag ccgctacgtt catcaactcc
     4021 cgaaggtgtt tctgacctcg ttcagtgacc gcatcgggaa aatatccctg ttcgttctcc
     4081 gctaacgtaa ccgatttcac ttcaatatag cagtctggac gcgaatccgc ctgcaacata
     4141 aagtcaatac ggctgcgttc tgcgccgtat tttacttcgc ttttcagcga gctatagcct
     4201 gacagttctg aaattgattc attaaggata gcctctttcg tcaacctgtt agcccaaagc
     4261 gtattgacgc aaataaatgc gccgctctgg ctttgagtta gttcccaggt gtgtgggtat
     4321 ttccgtttgg tgttgtctga agtcgaatac cagacggtat cgccacgcgt tgcacaaccg
     4381 gtcatcgcac ccgtattcgg gcagtgtagc gttaattcgc gaccatcggg tgtgatcaca
     4441 tcggctaaaa aacgtttgta acgctgaatt agcgtcgcgc gctgtagagg gggagagaat
     4501 tccattgcga caatccttgt tattgcgtta gcgcccagcg ttttagcggc gtgtagcgtg
     4561 tgcgtccacg ggcaaacgag gaggcgtaaa gggtgaactc cgtcaccgca taagaccagt
     4621 taaaacctgg cggcgggatt gtcaccgcct cgctggcgtc gcgcaataag gtaatatgtg
     4681 gatgaaacgg acgattgctt tgaaaacaac cgctgcgggc agcctgtgaa cggagcatat
     4741 tcgccagctg gattaagccg cgtggcggct gacgcatccc taaccacacc acacgcgaac
     4801 gcagccattg tccggcgtca tcaagcgtga gtgtgaaacc aggttgacga atccgtccgg
     4861 ctaaaagaga aagcgccttc tctttctcgg cgctcacttc gcctaaaaat gccagagtca
     4921 gatgcaaatt atcggcggcg accggacgtc ccgcctcagg tgggaagtgt gtggcgcgcc
     4981 agtggataat ctgttcgcgg atttctgcag gtaagtcgat agcaaagaac agacgttgcg
     5041 gttcagacat gtgaggcact cggttatgaa ttaccgcgat gctacaatgt ggcgcgaaga
     5101 atgttaaccc tctggagcgt tttgtgtcgt cgttgcccgt tgctgccgtc ttacctgaat
     5161 tacttaccgc tctcgattgt gcgccgcagg tattattaag tgcgccgacc ggggccggga
     5221 aatcaacctg gctgccgctg caactgctgg cgcatcccgg cattaacggg aaaattatcc
     5281 tgctggagcc gcgtcgtctg gcggcacgta acgtcgcgca acggctggcg gagctgctta
     5341 acgaaaagcc aggcgatacc gttggctacc ggatgcgtgc gcaaaactgc gtcgggccaa
     5401 atacccgtct ggaagtagtt accgaaggcg tgctgacgcg gatgatccag cgtgacccgg
     5461 agttgagcgg tgttggattg gtgatccttg atgaatttca tgagcgcagc ttgcaggcgg
     5521 atttggcgtt ggcgctgtta ctcgatgtgc aacaaggtct gcgtgatgac cttaaactgc
     5581 tgattatgtc ggctacgctg gataacgacc gcttgcagca aatgctgcca gaagcgcccg
     5641 tcgtcatctc agaagggcgc tcgtttccgg ttgaacgcca ttatttaccg ctgcccgcgc
     5701 atcagcgttt tgacgaagcc gttgcggtag ccaccgccga aatgctgcgt caggaaagcg
     5761 gatcattact gttattttta cctggcgtcg gagaaattca gcgtgtgcag gaacaactgg
     5821 cttcgcgcat cggcagtgat gtgttgctct gcccgctgta tggcgcgttg tcgctgaacg
     5881 atcagcgaaa agcgatcctc ccggcaccgc aagggatgcg caaagtggtg ctggcgacca
     5941 atattgctga aaccagttta accattgaag gtattcgtct ggtggtggat tgtgcccagg
     6001 agcgtgtggc gcgttttgag ccgcgcacgg ggcttacgcg actgattact caacgcgtta
     6061 gccaggcatc catgacgcag cgtgccgggc gcgccgggcg tctggagccg ggtatctgcc
     6121 tgcatttaat cgccaaagaa caagcagaac gcgctgccgc gcaaagtgaa ccggagatct
     6181 tacaaagcga tctttccggt ttgctgatgg aattactgca atggggatgc agcgatccgg
     6241 cgcagatgag ctggctggat caaccgccag tagtgaatct aatggccgcg aaacgtctgt
     6301 tacaaatgct gggggcgctg gacggtgaac ggcttagtgc gcaagggcaa aaaatggcag
     6361 cgctgggtaa cgatccgcgt ttagcggcaa tgctggttag cgcgaagaac gacgacgaag
     6421 ctgctaccgc ggcaaaaatt gccgccattc tcgaagagcc gccactgatg ggcaatagtg
     6481 acctgggcgt ggcgttttcg cgcaatcaac cagcctggca gcaacgtagt cagcaactgt
     6541 taaaacgctt aaacgtacgc ggcggtgagg cagacagttc gcttatcgcg ccgctacttg
     6601 ccggggcgtt tgccgatcgc attgctcatc gccgtgggca agatggacgc tatcaactgg
     6661 caaacggcat gggagcgatg ctcgatgccg acgacgcgct aagccgccac gaatggttga
     6721 tcgcaccgtt attattgcag ggcagcgcct cgccggatgc gcggatttta ctggcgctac
     6781 cggtcgatat tgatgaatta gtacaacgct gtccgcagct ggtgcagcaa tctgacaccg
     6841 tggaatggga tgacgcgcaa ggtacgctga aagcctggcg tcggctacaa atcggtcagt
     6901 tgacggtgaa agtgcagccg ctggcgaaac cgtcagaaga cgagttgcat caggcgatgc
     6961 ttaatggcat ccgtgataaa ggtttaagcg tgctcaactg gacggcggaa gcggaacagc
     7021 tacgcttgcg tttgttatgc gccgcaaagt ggttgccgga atatgactgg ccagcggttg
     7081 atgatgaaag tttattggca gcgctggaaa cgtggctgct gccacatatg acaggcgtac
     7141 attcactacg cggcctgaaa tcactggaca tttatcaggc actacgcgga ttacttgatt
     7201 ggggaatgca gcaacgtctg gatagtgaat tgcctgcgca ttacactgtg ccgacgggaa
     7261 gccggatcgc cattcgttat catgaagata acccgcccgc actggcggtg agaatgcagg
     7321 agatgtttgg tgaggccact aatccgacta tcgcccaggg gggcgtgccg ctggtgctgg
     7381 agttgctttc acccgcccaa aggccactac aaatcacacg agatttgagc gccttctgga
     7441 aaggagcgta ccgtgaggtg caaaaagaga tgaaagggcg ttatcccaaa cacgtctggc
     7501 cggacgaccc ggcaaacacg gcaccgacgc gacggacgaa aaagtattcg taaggttggt
     7561 tttct
//
LOCUS       DAZPJR010000193         7491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPJR010000193 DAZPJR010000000
VERSION     DAZPJR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.198"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHVB7_003822"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHVB7_003822"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR1915695.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVTYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="ACHVB7_003823"
     tRNA            647..722
                     /locus_tag="ACHVB7_003823"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHVB7_003824"
                     /gene_synonym="yqjH"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHVB7_003824"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR1915696.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHVB7_003825"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHVB7_003825"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR1915697.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHVB7_003826"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHVB7_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR1915698.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHVB7_003827"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHVB7_003827"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR1915699.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="ACHVB7_003828"
     CDS             complement(5917..6249)
                     /locus_tag="ACHVB7_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR1915700.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHVB7_003829"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHVB7_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR1915701.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1824 a         2029 c         1869 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat gtcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAZPJR010000194         7450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPJR010000194 DAZPJR010000000
VERSION     DAZPJR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.199"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1085)
                     /gene="ipaHd"
                     /locus_tag="ACHVB7_003830"
     CDS             complement(<1..1085)
                     /gene="ipaHd"
                     /locus_tag="ACHVB7_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHd"
                     /protein_id="HFR1915702.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDT
                     VSARNTSGFREQVAAWLEKLSASAELR"
     gene            complement(1686..1934)
                     /locus_tag="ACHVB7_003831"
     CDS             complement(1686..1934)
                     /locus_tag="ACHVB7_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1915703.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2197..2292)
                     /locus_tag="ACHVB7_003832"
                     /pseudo
     CDS             complement(2197..2292)
                     /locus_tag="ACHVB7_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2289..2855)
                     /locus_tag="ACHVB7_003833"
     CDS             complement(2289..2855)
                     /locus_tag="ACHVB7_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1915704.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            3165..4937
                     /gene="aspS"
                     /locus_tag="ACHVB7_003834"
     CDS             3165..4937
                     /gene="aspS"
                     /locus_tag="ACHVB7_003834"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR1915705.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            5055..5507
                     /gene="nudB"
                     /locus_tag="ACHVB7_003835"
     CDS             5055..5507
                     /gene="nudB"
                     /locus_tag="ACHVB7_003835"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR1915706.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            5536..6276
                     /locus_tag="ACHVB7_003836"
     CDS             5536..6276
                     /locus_tag="ACHVB7_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1915707.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            6311..6832
                     /gene="ruvC"
                     /locus_tag="ACHVB7_003837"
     CDS             6311..6832
                     /gene="ruvC"
                     /locus_tag="ACHVB7_003837"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR1915708.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6834..7436)
                     /locus_tag="ACHVB7_003838"
     CDS             complement(6834..7436)
                     /locus_tag="ACHVB7_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR1915709.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1886 a         1670 c         1968 g         1926 t
ORIGIN      
        1 tgtcgaagct ccgcagaggc actgagtttt tccagccatg cagcgacctg ttcacggaat
       61 ccggaggtat tgcgtgcaga gacggtatcg gaaaggcggt caaggaacgc ggaaaaggtg
      121 ttggcatgct cttcatgttc aaaagcatgc catatctgtg atacatcaga ttgtttgttt
      181 tccgggaacc atgctgtcac ggcatcagcc agggggcgat ggagtgtatt ctgttgtccg
      241 tcactcatgg agaagtaaat ctgcgggccg tggtagtccg gcgaagaggt taatctttgc
      301 agggattgca gagcatggga tgacaatggg ttatcactaa tatctattga acattcattc
      361 ctcagattaa gaatgctttc cgggatatga cttatctgat ttatgtcaaa ataaccgttc
      421 cttaaatttt ccggcaggta tggaatatcg gttaacatat tgtcgattgc ccatatggag
      481 tgaatgttgt gaggtagagg ggggagttca ctaagaagat tccctattgc gctaatctct
      541 tgtaaagaaa atggtaaagg tggtaaaact gccagcccat tacatgatac atctaatgtt
      601 tccagtagtt ctggtaaaac aggaagagaa cataattgat tatttgagac atgaagctcc
      661 ttcaaagata tggggagtgt gggtagtgtg attagttgat tgtgggacgc attcaataat
      721 ttaagtcctt gaggcaaagc aggcagttca ataagtctgt tatagctgac attaagctgt
      781 gtaagggacg caggcaatgg ggagattaag cttaaattat ttttacttat attaattgat
      841 ttaatttccg gggggatttc aggtaatgtt gtcaggccta attcagacaa gtccaggttc
      901 gtctcttggt tttgtagaca tgatactagt cgctgaaaag cgatgtctcg ttgttcttct
      961 tgtatgcggt tatttttcca ttcagtccaa tgggttagat aattttcata tgcacggcta
     1021 gtgtcgattg aataagtgga gaacgaattt gaaattaatc tgtgattgtt atttgtcggg
     1081 agcataaata tcagggcgta ttctttatgt tgagaggtgt tggattcttt tttatctgct
     1141 ttgcaatgtt attggttccc ttcagcagta gacaggaact tctggaggag gcgaatatat
     1201 agtttgtgta agcatattaa ctccatatgt tatatattga agaacttctg ctttacacta
     1261 tcctaaactg gatcggttta atgtaaaaaa acgatacttt ttgggggagg gggcaatttt
     1321 tccttaaggt atgaaaaaag gagctgaagc tatataatag ctattaatgc cactggtatc
     1381 agtgaatcat gtatataaaa aactgtgtta aggaggctgt ccattagcgg ttttttattc
     1441 ttctggaacc gcggcatctt tgcgtatctt tttgtcttct caccgtctgg tcggtgtttt
     1501 gctgagactg ctaatttcct gtctttgcta gtgctgtact ggtactgccc aatcatgatt
     1561 ggcgggggcg ggcggagttg agaccgcaga cacgtcgtat gcaagaacgt gctgcggctg
     1621 gctggtgaac ttccgatagt gcgagtattg aatgatttcc agccgttact gattttacgt
     1681 gctaattagt gaacaaacca ctcgtcagca gactcccagg tatctttcag agtttcctga
     1741 acaaaagttt tagctgaatc tttatcggcg gtgcgcgtaa cagaaaggcc atcgttgctg
     1801 gtggctttta cgatcacctc tacatcgtca taacgtttac tgatgcgtcg ggttaattct
     1861 tcctttaacg catccacagc accggttggc attttagtca ttttctcttt ggctatgcag
     1921 atttcaatac gcataaaaat ccctctacac tgtgttttta tacagtatta tttttaactg
     1981 tatggataaa cagtgtcaag agtgagggaa agtgattttt tggggcatgc atggggcaaa
     2041 aatatttgtt gtggggcatt ttggggcatg attgggacac tttgttacat atgaactttg
     2101 ccgattttca tatggcatgg caatccaacg ctttgattat tctaaagaaa acacatgctc
     2161 ttgggcgttc tttagtgatt tttaaaaatc ccgtatcacg cagttaaagt ggcgggcata
     2221 ctcttcaagg ctggtgatcc ccaaccgcac ccatttagga tgcgaccatt gcggtagacc
     2281 gatgtaaatc ataacgcgtg aaggatctct tccacgctac gcacacgggc gatgcgcggg
     2341 tagatatgat taatgctgtt attgtgttgc tcggcgctgg cggcactaca ggcgtcttcg
     2401 gcaatcacca gattaaagcc aagttcccag gcattgcggg cggtggattc aacaccgata
     2461 ttggtcgaga tcccacataa cactattgta tcgatacctc ggcggcgcag ttgcaactcc
     2521 agatccgtac cgtaaaacgc accccactga cgtttgatga tttcgatatc actgtcagtt
     2581 gcacctaatg cagctggatg ttgccaccag ttttcgggca acactttagc gggggagggg
     2641 gcatcaaccg gctgttttaa tgcttcggcg taatcggcag accagccaac tcgcaccaga
     2701 aacacgggct gaccgctggc gcgaaatttc gccgccagct tcccggcgcg attaactacc
     2761 tcatcggcag tatgtggacc accggcaaaa ggtaagatgc cttcttgtaa atcgatcacc
     2821 accaacgcag tggttttagc attaagttca agcatgctga ctcctgaaaa aacaaaatcg
     2881 tgcctcacac cttaatgcga ttagccgatg gcggaacgac aaattttgtt aatttttatg
     2941 agttcgggca acacctttat agcaaactgg cgtagagtct gatttcaggc ccgatataaa
     3001 gtggtaacga ggcgttacag cgcgggccag gctcttacgg tgcggcagaa tttccagtat
     3061 aatagccgcc ttttttcatt cagttgtgac atacagctaa cgctgcgact tggtcacctg
     3121 cgcagcaggg ggcatccgcc ggcagctaag ttaagggata tctcatgcgt acagaatatt
     3181 gtggacagct ccgtttgtcc cacgtggggc agcaggtgac tctgtgtggt tgggtcaacc
     3241 gtcgtcgtga tcttggtagc ctgatcttca tcgatatgcg cgaccgcgaa ggtatcgtgc
     3301 aggtattttt cgatccggat cgtgcggacg cgttaaagct ggcctctgaa ctgcgtaatg
     3361 agttctgcat tcaggttacg ggcaccgtac gtgcgcgtga cgaaaaaaat attaaccgcg
     3421 atatggcgac cggcgaaatt gaagtgctgg cgtcctcgct gactatcatc aaccgcgcag
     3481 atgttctgcc gcttgactct aaccacgtca acaccgaaga agcgcgtctg aaataccgct
     3541 acctcgacct gcgtcgcccg gaaatggcgc agcgcctgaa aacccgcgct aaaatcacca
     3601 gcctggtgcg ccgttttatg gatgaccacg gcttcctcga catcgaaact ccgatgctga
     3661 ccaaagccac gccggaaggc gcgcgtgact acttggtgcc ttctcgtgtg cacaaaggta
     3721 aattctacgc actgccgcaa tccccacagt tgttcaaaca gctgctgatg atgtccggtt
     3781 ttgaccgtta ctatcagatc gttaaatgct tccgtgacga agacctgcgc gctgatcgtc
     3841 agcctgaatt tactcagatc gatgtggaaa cttctttcat gaccgcgccg caagtgcgtg
     3901 aagtgatgga agcgctggtg cgtcatttgt ggctggaagt gaagggcgtg gatctgggcg
     3961 atttcccggt aatgaccttt gcggaagcag aacgccgtta tggttctgat aaaccggatc
     4021 tgcgtaaccc gatggaactg accgacgttg ccgatctgct gaaatctgtt gagtttgcag
     4081 tatttgcggg gccggcgaac gatccgaaag gccgtgttgc cgctctacgt gttccgggtg
     4141 gcgcatcgct gacccgtaag cagatcgacg aatacggtaa cttcgttaaa atctacggcg
     4201 cgaaaggtct ggcttacatc aaagttaacg aacgcgcgaa aggtctggaa ggtatcaaca
     4261 gcccggtagc gaagttcctt aatgcagaaa tcatcgaagc catcctggat cgtactgccg
     4321 cgcaagatgg cgatatgatt ttcttcggtg ccgacaacaa gaaaattgtt gccgacgcga
     4381 tgggcgcact gcgcctgaaa gtgggtaaag accttggtct gaccgacgaa agcaaatggg
     4441 caccgctgtg ggttatcgac ttcccgatgt ttgaagacga cggtgaaggc ggcctgacgg
     4501 caatgcacca tccgttcacc tcaccgaaag acatgactgc tgcagaactg aaagctgcac
     4561 cggaaaatgc ggtggcgaac gcttacgata tggtcatcaa tggttacgaa gtgggcggtg
     4621 gttcagtacg tatccataat ggtgatatgc agcagacggt gtttggtatt ctgggtatca
     4681 acgaagagga acagcgcgag aaattcggct tcctgctcga cgccctgaaa tacggtactc
     4741 cgccgcacgc aggtctggca ttcggtcttg accgtctgac catgctgctg actggcaccg
     4801 acaatatccg tgacgttatc gccttcccga aaaccacggc ggcagcgtgt ctgatgactg
     4861 aagcaccgag ctttgctaac ccgactgtac tggctgagct gagcattcag gttgtgaaga
     4921 aggctgagaa taactgatat gactcaaata cacgaaatca ttcgcgttgc atcaaggcgg
     4981 caactgagtg aactcccatg agcatagata actatgtgaa tgggatgagc gaaggcagtc
     5041 aacgaagagg cagcgtgaag gataaagtgt ataagcgtcc cgtttcgatc ttagtggtca
     5101 tctatgcaca agatacgaaa cgggtgctga tgttgcagcg gcgtgacgat cccgatttct
     5161 ggcagtcggt aaccggcagc gtggaagagg gtgaaaccgc gccgcaagct gccatgcgcg
     5221 aagtaaagga agaggtcacc attgatgttg tcgctgaaca actgacctta attgactgtc
     5281 agcgcacggt agagtttgaa attttttcac atttacgtca tcgctatgcg ccgggcgtga
     5341 cgcgtaatac ggaatcatgg ttctgtcttg cgctcccgca cgaacgccag gtagtcttca
     5401 ctgaacatct ggcttacaag tggcttgatg cgcctgctgc cgcggcgctc actaagtcct
     5461 ggagcaaccg gcaggcgatt gaacagtttg taattaacgc tgcctgaaca ggcagggcta
     5521 tctggagata tttttatggc aggtcatagt aaatgggcca acaccagaca tcgtaaagct
     5581 gcgcaggatg ctaagcgcgg taaaatcttc actaaaatca ttcgtgagct ggtaaccgcg
     5641 gctaagctgg gcggtggcga tccggacgct aacccgcgtc tgcgtgcggc ggtggataaa
     5701 gcactgtcta acaacatgac ccgtgacaca ctgaaccgcg caattgcacg tggtgtgggc
     5761 ggtgatgatg atgcaaacat ggaaaccatc atctacgaag gttacggtcc tggcggcacg
     5821 gcaatcatga ttgaatgtct gtctgacaac cgcaaccgta ccgttgctga agtgcgtcat
     5881 gcatttagca aatgtggcgg taacctcggt actgatggtt ccgttgccta tctgttcagc
     5941 aaaaaaggcg tgatctcctt cgagaaaggc gatgaagaca ccattatgga agcagcactg
     6001 gaagcaggtg ctgaagacgt tgtgacctat gatgacggcg cgattgacgt ctacactgca
     6061 tgggaagaaa tgggtaaagt gcgcgacgct ctggaagcgg caggtctgaa agcagacagc
     6121 gcggaagttt ccatgatccc gtccaccaaa gctgatatgg atgcagaaac cgcaccgaaa
     6181 ctgatgcgtc tgatcgatat gctggaagat tgcgacgacg tgcaggaagt ctaccataac
     6241 ggtgaaatct ctgatgaggt tgcagcaact ctctgatggg gcctgctaaa cagcaaaacg
     6301 gagacgcgtg atggctatta ttctcggcat tgacccgggt tcgcgcgtga tcggctatgg
     6361 tgtcatccgc caggtaggca ggcaactgtc ctacctgggt agcggatgca tacgcaccaa
     6421 agtggatgat ttaccgtctc gtctgaagct catctatgcg ggcgtgacgg aaatcatcac
     6481 ccagttccag ccagattatt tcgccattga acaagtcttt atggccaaaa atgccgactc
     6541 ggctctgaaa ctggggcagg cgcgcggcgt ggcgattgtg gcggcggtga atcaggaatt
     6601 gccggtattt gaatacgcgg cacgtcaggt aaagcaaacg gtggtaggta ttggtagtgc
     6661 cgaaaaaagc caggtgcagc atatggtccg caccttgctg aaactgcccg ctaatccaca
     6721 ggcggatgcc gccgatgcgt tagctatcgc gattacccat tgtcacgtca gccagaatgc
     6781 aatgcagatg agcgaatcgc ggctgaacct ggcgagaggg cgactgcgtt aagttatacc
     6841 gcctcggtca gttcaggctg aggcgtttca actccctccg cgtgaatcaa caccagaccc
     6901 ggatgatgcc acaaactggc tggcgtgacg gttttacgct cccacggaat agaacctaag
     6961 aaccagaatt tccagaaagt gagttttgca ttcggattgc tatttaacag ttctccaaac
     7021 gtttctattt cacccaccgg aatgcatagc gcttctttat aaatatcaaa aacaaatttt
     7081 gaacagaact ggcgcgaaga ttcgtattta aaaccggtgt ggtaaagttt gcgtagccgg
     7141 gagggaacct gttcaacaat tcgttgtttt tgttgttctg tcagcccagc gtctaatcgc
     7201 tttatagcat agcgttgatt agacgaacgt ttaataaaac gagatagcgt ggtgatggtt
     7261 gaaaggggaa cccggctttc tgcaaccagg aagtcttcac cgttatgacc gataatgatc
     7321 ccgacgtgat tactccagca atttgatgca gctgatattt gaccaaataa ggcagcacct
     7381 atacatgtaa agacgatatc tccaatttca tattcagcag ggtaattaat attcacgttg
     7441 tcattccatt
//
LOCUS       DAZPJR010000195         7429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPJR010000195 DAZPJR010000000
VERSION     DAZPJR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.200"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..99)
                     /locus_tag="ACHVB7_003839"
     CDS             complement(<1..99)
                     /locus_tag="ACHVB7_003839"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HFR1915710.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAEE"
     gene            complement(124..1224)
                     /locus_tag="ACHVB7_003840"
     CDS             complement(124..1224)
                     /locus_tag="ACHVB7_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR1915711.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="ACHVB7_003841"
     CDS             complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="ACHVB7_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR1915712.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2372..2938)
                     /locus_tag="ACHVB7_003842"
     CDS             complement(2372..2938)
                     /locus_tag="ACHVB7_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1915713.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3154..4890
                     /gene="argS"
                     /locus_tag="ACHVB7_003843"
     CDS             3154..4890
                     /gene="argS"
                     /locus_tag="ACHVB7_003843"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR1915714.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(4932..4976)
                     /gene="yecV"
                     /locus_tag="ACHVB7_003844"
     CDS             complement(4932..4976)
                     /gene="yecV"
                     /locus_tag="ACHVB7_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR1915715.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            5067..5429
                     /locus_tag="ACHVB7_003845"
                     /pseudo
     CDS             5067..5429
                     /locus_tag="ACHVB7_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5433..6503)
                     /locus_tag="ACHVB7_003846"
                     /pseudo
     CDS             complement(5433..6503)
                     /locus_tag="ACHVB7_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6496..>7429)
                     /gene="flhB"
                     /locus_tag="ACHVB7_003847"
     CDS             complement(6496..>7429)
                     /gene="flhB"
                     /locus_tag="ACHVB7_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR1915716.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1920 a         1947 c         1776 g         1786 t
ORIGIN      
        1 ctcttctgcc catgcgggga gcggtgctgc cgatgtcacc acctgcgctc cagcggcaat
       61 accgctgtgt ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga
      121 ggatcactgt ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc
      181 cagttcgttt tcgctcatgc tggttcgtgc ccccattcct ttggcaatgg acggccacgc
      241 attgacggtg taatggtcgg cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc
      301 aagtttttca gcgtattgcc atagcggttt acggtccgct aatccgggat cggtaagcgc
      361 accctgtaaa gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt
      421 taccgtgagc gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc
      481 cgccaggtag agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg
      541 ctgtttgcca ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc
      601 gcctatgcga gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggc
      661 ctgactttcc agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc
      721 aatgccttta tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt
      781 ctgcgcagat tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca
      841 cgttgcagag tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg
      901 agtttcgaac ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt
      961 agatgctttt aatttagcaa ataaataatc catccctgac tttggaatat ggcaatctgc
     1021 acattccgca cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa
     1081 agatttactc atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc
     1141 tttttgcgcc agcaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa
     1201 cccaatgcgt tttttccctc gcatagattc ctctaaataa attccagaac accggaatca
     1261 ttgtataatt aaatacacaa cgaattaacc gcttgcataa ctcggcacaa cactgcctga
     1321 aataatcgat aaagaatatg atttattaca atgtaatcat taattgctaa ggaataaccc
     1381 agttgctatt tagaatatat atttatcagt tttagcaatt taaatcccat aatcaatgtg
     1441 aatataaaaa atattattag cacaccgcta tgttcactgt cacccgctca acagatcacg
     1501 gttatcgttc gttttttata ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg
     1561 ggcctggtac gagcaagcat catattgggc gacatgatgc aacggtaaaa atcatttggc
     1621 ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc gccccgtcta cgatataacg
     1681 cgaatactcg tccgcgtgtt catctgatga catggacaat ccttgattac gataacgcat
     1741 cggtgaggct tgccacgctc ccgcggagct atggacttcc agcactccgg catcgaggaa
     1801 gtggtgcagg ttttctgcac ggactcctgc tccggccata atgattggag catcacgatg
     1861 ggcaataagt tccataattt ttgataaacc ttgcagtgcg tctgattttt gccctgatgt
     1921 cagtacccgg gcaatgccca attccgcaag attattgagt gtatataaag ggttagcgca
     1981 catatcgaag gcgcgatgaa aagtcactgc cagcggaccg gcagcagcca ttattttttc
     2041 cattcgtggc atatcgacat tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa
     2101 acctaattcg cggaccgtgc gcacatcctc aagaatggcg gcaaactcac cgtcgctgta
     2161 gcaaaaatca ccaccgcgtg ggcgaattat cggatgcaca gggatcgtca cccgctggcg
     2221 cacggatttc agtaccccca acgacggcgt taagcccccc tcttttgggg ctgcgcataa
     2281 ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca cattccatgc tgtaacagca
     2341 aatttccagt aatgccattt ttgctcctta attacgctga ctgctcgctg gcaacgatct
     2401 cttcaatgga ccacggatga aacttaatag ttgttttgcc atcggtgacc gccagcgtcg
     2461 gattcggtaa tcgctcatgc tcaccttttg gtgaactggt tttaactgag atccccggta
     2521 ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc attcagcgtt tctggatcac
     2581 ccagcagaac gatttcaata tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc
     2641 acggtagctc cacaatagaa aactgccagt gcgcaacctg taccggttca tgcaatttaa
     2701 acagacaaat cggtctgcca ttgatcatat tttctgacaa aagctcgcca cactgttcaa
     2761 acccgcgacg ccagcgttca gcagtggcgt tttgatggca acgcaaagaa atgtgatcgg
     2821 cagtcagcgg agtgatattc aaccccagac ggagggaaag ttcatctaat gcgtgggtaa
     2881 atcgcggtaa atccgatgca atatcctgca gctcgtcgat agattgccag ttcgccataa
     2941 tcactcttcg tctttcagta aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg
     3001 ctgatttcac gccgcttctg atgcaatagt gaaaacggca atacgccacg cgcacgttgc
     3061 tgacgaaaac agccatttgc agtatactcc cgccctaatt tctttaactg gtgcgggcaa
     3121 tttttgctcg cttcatcaat gtaaggtatt ccggtgaata ttcaggctct tctctcagaa
     3181 aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt
     3241 cattcagcaa aagttcagtt cggcgactat caggctaacg gcatgatggc agttgctaaa
     3301 aaactgggta tgacaccgcg acaattagca gagcaggtgc tgactcatct ggatcttaac
     3361 ggtatcgcca gcaaagttga gatcgccggt ccaggcttta tcaacatttt ccttgatccg
     3421 gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg
     3481 ccagaaaaac agaccattgt ggttgactac tctgcgccaa acgtggcgaa agagatgcat
     3541 gtcggtcacc tgcgctctac cattattggt gacgcagcag tgcgtactct ggagttcctc
     3601 ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg gcactcagtt cggtatgctg
     3661 attgcatggc tggaaaagca gcagcaggaa aacgccggtg atgaaatgga gctggctgac
     3721 cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aagatgaaga gtttgccgag
     3781 cgtgcacgta actacgtggt aaaactgcaa agcggtgacg aatatttccg cgagatgtgg
     3841 cgcaaactgg tcgacatcac catgacgcag aaccagatca cctacgatcg tctcaacgtg
     3901 acgctgaccc gtgatgacgt gatgggcgaa agcctctaca acccaatgct gccaggaatt
     3961 gtggcggatc tcaaagccaa aggtctggca gtagaaagcg aaggggcgac cgtcgtattc
     4021 cttgatgagt ttaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat
     4081 ggcggctatc tctacaccac cactgatatc gcctgtgcga aatatcgtta tgaaacactg
     4141 catgccgatc gcgtgctgta ttacatcgac tcccgtcagc atcaacacct gatgcaggca
     4201 tgggcgatcg tccgtaaagc aggctatgta ccggaatccg taccgctgga acaccacatg
     4261 ttcggcatga tgctgggtaa agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg
     4321 aaactggccg atctgctgga tgaagccctg gaacgtgcac gccgtctggt ggcagaaaag
     4381 aacccggata tgccagctga cgagctggaa aaactggcta acgcggttgg tattggtgcg
     4441 gtgaaatatg cggatctctc caaaaaccgc accacggact acatcttcga ctgggacaac
     4501 atgctggcgt ttgagggtaa taccgcgcca tacatgcagt atgcttacac gcgtgtattg
     4561 tccgtgttcc gtaaggcaga aattaacgaa gagcaactgg ctgcagctcc ggtaatcatc
     4621 cgtgaagatc gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcacc
     4681 gtggttgccc gtgaaggcac gccgcatgtg atgtgtgctt acctgtacga tctggctggt
     4741 ctgttctctg gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt
     4801 aacagccgtc tgaaactggc acaactgaca gcgaagacgc taaagctggg tctggatacg
     4861 ctgggtattg agacagtaga gcgtatgtaa tcgatttttc gtgagagtaa agcctgatca
     4921 gggttacctg atcaggcttt tttattgcca tctaaatgta ttctgaaaat ggacatgcca
     4981 ttgttttctg actgttggat aagaggccag aagcgtaata tccggcccca gggaaacgat
     5041 aacggttgaa tttaaggaat accgcagtgt ttaaatttct tgtattaaca ttaggcatta
     5101 tctcttgcca ggcttacgca gaagatacgg ttatagaaaa cgaccatgac atttcagcca
     5161 tcaaagattg ttggcaaaaa aattcagatg atgatactga cattaacgtg atcaaatcat
     5221 gcctgcgaca agaatacaat ctcgtcgatg cgcaattaaa taaagcctat ggtgaagctt
     5281 atcgttatat agaacaagtg ccacgcacag gtgtaaaaaa acctgatacc gaacaactta
     5341 acttgcttaa aaaatcacag cgagcctggc tggattttag ggacaaagaa tgtgaattaa
     5401 tcctttcaaa tgaggacgtt caggatttaa tgtacatcgt tccacgtcgc ttcgacaacg
     5461 gtattattct ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg
     5521 cgtatccacc aggccagccc gagcaatccg gcagtgaaca gcaaaaatac caggttcggc
     5581 attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg
     5641 ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta
     5701 acgatgaccc ccgcggcggt aaaaatcacc agcgccggaa tttgtgccac cagaccatcg
     5761 ccaatggtca atagcgtata actttccgcc gcgtgtccca tgctcatgcc atgttgcagc
     5821 acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc ggcgatggca
     5881 tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc ttcctgagtc
     5941 acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccagcgtt aaggtcggcg
     6001 tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc cacttctgcg
     6061 atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat gacaaacacc
     6121 acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc gaccaccttc
     6181 cctgccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga agccacgtta
     6241 agtgccagac gcaacaacgt agtaaacaac agaatggtcg gaaacgcagc aaactcaagc
     6301 gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat attgaaggta
     6361 aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa gatcaacagg
     6421 atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc gggcaggcgc
     6481 agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca gggcttccgg
     6541 caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc agcgtttcag
     6601 ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg
     6661 accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa gcgtcgggac
     6721 gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac ctttagcgac
     6781 cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat agtgggtcgg
     6841 gttattgaca atgacatcag ctttcggcac atcggccatc atccgacgcc gtgcagcagc
     6901 tcgctgcatc tgacggatcc gccctttaac atgagggtca ccttcgcttt gtttgaactc
     6961 atcacgaata tcctgccgcg acatacgcag ctttttcagg tggctgaaga tttggaaaaa
     7021 gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc atagcccaac
     7081 cagatccatc gcattaccca tggcggtagt cggagactcg gccattaagc gcatcatctg
     7141 cggccagtga tgccagagat aaaaccccgt cacgctgcca accaggatgg ttttcaaaat
     7201 tgccttaagc aactccgcgc cagtctgtgc cgagaacatc cgtttaatgc ccggtagcgg
     7261 gttgagtttg gaaaacttcg gctgcaagga tttgccgcta aataccagcc cccccagcat
     7321 gaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggcagca gcgccagcat
     7381 ggcttctctg atcagcagaa taatctgccc gaggatcaga ttcgggtca
//
LOCUS       DAZPJR010000196         7424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPJR010000196 DAZPJR010000000
VERSION     DAZPJR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.201"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(82..1590)
                     /gene="putP"
                     /locus_tag="ACHVB7_003848"
     CDS             complement(82..1590)
                     /gene="putP"
                     /locus_tag="ACHVB7_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR1915717.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2012..5974
                     /gene="putA"
                     /locus_tag="ACHVB7_003849"
     CDS             2012..5974
                     /gene="putA"
                     /locus_tag="ACHVB7_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1915718.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6014..6652)
                     /gene="rutR"
                     /locus_tag="ACHVB7_003850"
     CDS             complement(6014..6652)
                     /gene="rutR"
                     /locus_tag="ACHVB7_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR1915719.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6940..>7424
                     /locus_tag="ACHVB7_003851"
     CDS             6940..>7424
                     /locus_tag="ACHVB7_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1915720.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1774 a         2056 c         1943 g         1651 t
ORIGIN      
        1 cattcaattt atatggcgag gcacattaag ggagcgcaac ccgctgctga agccaaacaa
       61 aaccgccgca ggctaagtcc cttagttttc ctgcaaccgt gacggcggag ccgaatgata
      121 gtgcgcatcg gcctcggcaa agcgtttttg catcgccgct gacggcgctt tacccagcaa
      181 actaaacact acaatcccga tactgccgaa gatgaagccc ggaataattt cgtacagtcc
      241 cagccagccg aactgcttcc agacgataac tgtcagcgca ccgatgatca tccccgccag
      301 cgcaccgttg cgcgtcatgc gtgaccacat caccgagaac agcaccaccg gaccaaacgc
      361 cgcgccaaag cctgcccacg cgtagctcac taagcccagc acgcggtttt ccgggtttgc
      421 cgccagcgca atcgccacca gcgccaccac cagcaccatc acacgtccta cccacaccag
      481 ctctttctgg ctggcctgtt tacgcagaaa cgctttgtat aaatcttcgg taatcgcact
      541 ggagcacacc agcagctggc aacttaaggt tgacattacc gccgccagaa tcgccgacag
      601 cagaatcccg gcaatccacg ggttaaacag aatttgcgcc agttcgataa acacgcgttc
      661 agcgttctgg tttaccgcac ctgctaccgc cggatgctcg ttaaagtaag cgataccaaa
      721 gaagccaaca gccaccgccc ctgccaggca aaggatcatc caggtcatac tgatacgacg
      781 cgcatggaca atgctgtggt gagaatctgc cgccataaaa cgcgccagga tgtgcggctg
      841 cccgaagtaa cccagccccc aacccatcaa tgagataatg gcaacaaagt tcagcccttt
      901 gagcatatcc acgttttcga tgctcttttg tttgatcact tccagcgagt caccaaagcc
      961 accgacactg ataatgacga taaccggcgt caggatcagg gcaaaaatca tcaggctggc
     1021 ctgtacagtg tcagtccagc tcaccgcgag gaaaccgcca ataaaggtgt aaaggatcgt
     1081 cgacgcagct cccgccaaca gagccgtttc gtaactcatg ccaaaggtac tttcaaacag
     1141 acgcgcgcct gccacaatgc ccgaagcgca ataaatggtg aagaacagca aaataaccag
     1201 cgcggagata atgcgcaaaa tgcggctttt atcttcaaaa cgcccggtga aataatccgg
     1261 caatgttaag gcgttattat tgtattcggt atgcacacgc aaccgcccgg ccaccagctt
     1321 ccagttgatc cacgcgccta atgtcaggcc aatggcgatc cagctttcgg aaatcccgga
     1381 aagaaaaacc gcgccaggca accccattaa cagccagccg ctcatatccg acgcacccgc
     1441 cgataatgcc gtcacgaatg gcccaagact acgaccgccc agaatatagt cgtcaaagtt
     1501 tttcgttgat cgccaggcga taaacccaat caatatcatg ccaaagatat agacacaaaa
     1561 tgtcaccaac atcggtgtgc taatagccat ctaaagtctc caaaaaatta ttatcggcaa
     1621 tgtcgaaact tgccgttata tctgccaccg gaacggggta acagagttta tgttttacca
     1681 gggcgaccgt atcctgccgg aagcgctggt tattcacaat cgatttaaca caccatttac
     1741 attaaatttt agtgctcagc gacactattt ttcatcaggt tgcactctct cacatttttt
     1801 gcggttgcac ctttcaaaaa ggttaactgc cgcagagaaa aagtctgagt tattttttaa
     1861 tcactgtcat atcgatttct tttattaaca tttcattcat ttttaagctt gctacgcaag
     1921 tcacatttaa catggttgca caaagttgca acatcatgga tatttcacga taacgttaag
     1981 ttgcaccttt cagaacaaca ggagtaatgg catgggaacc accaccatgg gggttaagct
     2041 ggacgacgcg acgcgtgagc gtattaagtc tgccgcgaca cgtatcgatc gcacaccaca
     2101 ctggttaatt aagcaggcga ttttttctta tctcgaacaa ctggaaaaca gcgatactct
     2161 gccggagcta cctgcgctgc tttctggcgc ggccaatgag agcgatgaag caccgactcc
     2221 ggcagaggaa ccacaccagc cattcctcga ctttgccgag caaatattgc cccagtcggt
     2281 ttcccgcgcc gcgatcaccg cggcctatcg ccgcccggaa accgaagcgg tttctatgct
     2341 gctggaacaa gcccgcctgc cgcagccagt tgctgaacag gcgcacaaac tggcgtatca
     2401 actggccgat aaactgcgta atcaaaaaaa tgccagtggt cgtgcaggaa tggtccaggg
     2461 gttattgcag gagttttcgc tgtcatcgca ggaaggcgtg gcgctgatgt gtctggcaga
     2521 agcgttgttg cgtattcccg acaaagccac ccgcgacgcg ttaattcgcg acaaaatcag
     2581 caacggtaac tggcagtcac acattggtcg tagcccgtca ctgtttgtta atgccgctac
     2641 ctgggggctg ctgtttactg gcaaactggt ttccacccat aacgaagcca gcctctcccg
     2701 ctcgctgaac cgcattatcg gtaaaagcgg tgaaccgctg atccgcaaag gtgtggatat
     2761 ggcgatgcgc ctgatgggcg agcagttcgt cacgggtgaa accatcgcgg aagcgttagc
     2821 caatgcccgc aagctggaag agaaaggttt ccgttactct tacgatatgc tgggcgaagc
     2881 tgcactgact gccgcagacg cacaggcgta tatggtttcc tatcagcagg cgattcacgc
     2941 cattggtaaa gcgtctaacg gtcgtggcat ctatgaaggt ccgggcattt caatcaaact
     3001 gtcggcgctg catccgcgct acagccgcgc ccagtatgac cgggtaatgg aagagcttta
     3061 cccgcgtctg aaatcactca ccctgctggc gcgtcagtat gatattggta ttaacatcga
     3121 tgccgaagag gccgatcgtc tggagatctc cctcgatctg ctggaaaatc tctgtttcga
     3181 gccggaactg gcaggctgga acggcatcgg ttttgttatt caggcttatc aaaaacgctg
     3241 cccgttggtg atcgattacc tggttgatct cgccacccgc agccgtcgcc gtctgatgat
     3301 tcgcctggtg aaaggcgcgt actgggatag tgaaatcaag cgtgcgcaga tggacggcct
     3361 tgaaggctat ccggtttata cccgcaaggt gtataccgac gtttcttatc tcgcctgtgc
     3421 gaaaaagctg ctggcggtgc cgaatctaat ctacccgcag ttcgcgacgc acaacgccca
     3481 tacgctggca gcgatttatc aactggcagg tcagaactac tatccgggtc agtacgagtt
     3541 ccagtgcctg catggtatgg gcgagccact gtacgagcag gtcaccggga aagttgccga
     3601 cggtaaactt aaccgaccgt gtcgtattta tgccccggtt ggcacacatg aaacgctgct
     3661 ggcgtatctg gtgcgtcgcc tgctggaaaa cggtgctaac acctcgtttg ttaaccgtat
     3721 tgccgacacc tctttgccac tggatgaact ggtcgccgat ccggtcactg ctgtagaaaa
     3781 actggcgcaa caggaagggc aaactggatt accgcatccg aaaattcccc tgccgcgcga
     3841 tctttacggt cacgggcgcg acaactcggc agggctggat ctcgctaacg aacaccgtct
     3901 ggcctcgctc tcctctgccc tgctcaatag tgcactgcaa aaatggcagg ccttgccaat
     3961 gctggaacaa ccggtagcgg caggtgagat gtcgcccgtt attaaccctg cggaaccgaa
     4021 agatattgtg ggctttgtgc gtgaagccac gccacgtgaa gtagaacagg cgctggaaag
     4081 tgcggttaat aacgcgccaa tctggtttgc cacgcctccg gctgaacgcg cggcgatttt
     4141 gcatcgcgct gccgtgctaa tggaaagcca gatgcagcaa ctgattggta ttctggtgcg
     4201 tgaagccgga aaaaccttca gtaacgccat tgccgaagtg cgcgaagcgg tcgattttct
     4261 ccactactac gccggacagg tgcgggatga tttcgctaac gaaacccacc gtccattagg
     4321 gcctgtggtg tgtatcagtc cgtggaactt cccgctggct attttcaccg ggcagatcgc
     4381 tgccgcactg gcggcaggta acagcgtgct ggcaaaaccg gcagaacaaa cgccgctgat
     4441 tgccgcgcaa gggatcgcca ttttgctgga agcgggtgta ccccctggcg tggtgcaatt
     4501 gctgccaggt cagggggaaa ccgtgggcgc gcaactgacg ggtgatgatc gcgtgcgcgg
     4561 ggtgatgttt accggttcaa ccgaagtcgc tacgttactg cagcgcaata tcgccagccg
     4621 cctggacgct cagggtcgcc ctattccgct catcgctgaa accggcggca tgaacgcgat
     4681 gattgtcgat tcttcagcac tgaccgaaca agtcgtcgtg gatgtgctgg cctcggcgtt
     4741 cgacagtgcg ggtcagcgtt gttcggcact gcgcgtgctg tgcctgcaag atgagattgc
     4801 cgaccacacg ttgaaaatgc tgcgcggtgc aatggccgaa tgccggatgg gtaatccggg
     4861 tcgcctgacc accgatatcg gtccggtgat tgatagcgaa gcgaaagcca atattgagcg
     4921 ccatattcag accatgcgta gcaaaggccg tccggtgttc caggcggtgc gggaaaacag
     4981 cgaagatgcc cgtgaatggc aaagcggcac ctttgtcgcc ccgacgctga tcgaactgga
     5041 tgactttgcc gaattgcaca aagaggtctt tggtccggtg ctgcatgtgg tgcgttacaa
     5101 ccgtaaccag ctaccagagc tgatcgagca gattaacgct tccggctatg gtctgacgct
     5161 tggtgtccat acgcgtattg atgaaaccat cgcccaggtc actggctcgg cccatgttgg
     5221 taacctgtat gttaaccgta atatggtggg cgcagtggtt ggtgtgcagc cgttcggcgg
     5281 cgaagggttg tccggtaccg ggccgaaagc aggcggtccg ctctatctct accgtctgct
     5341 ggcgaatcgc ccggaaagtg cgctggcagt gacgctcgcg cgtcaggatg cagagtatcc
     5401 ggtcgatgca cagttgaaag ccgcattgac tcagccgcta aatgcactgc gggaatgggc
     5461 agcaaatcgt ccagaattgc aggcgttatg tacgcaatat ggcgagctgg cgcaggcagg
     5521 aacacaacga ttgctgccgg ggccgacggg tgaacgcaac acctggacgc tgctgccgcg
     5581 tgagcgcgtg ttgtgtattg ccgatgatga gcaggatgcg ctgactcagc tcgccgccgt
     5641 gctggcggtg ggcagccagg tactgtggcc ggatgacgcg ctgcatcgtc agctagtaaa
     5701 ggcattgcca tcggcagtca gcgaacgtat tcaactggcg aaagcggaaa atataaccgc
     5761 tcaacccttt gatgcggtga tcttccacgg tgattcggat cagcttcgcg cattgtgtga
     5821 agccgttgct gcgcgggatg gcgcaattgt ttcggtgcag ggttttgccc gtggcgaaag
     5881 caatatcctt ctggaacggc tgtatatcga acgttcgctg agtgtgaata ccgctgccgc
     5941 tggcggtaac gccagtttaa tgacgatcgg ttaaacaggt agccggagga tgttacaacc
     6001 tcctccggca tctttaacgt ggtcgaatcc cctcaataat aatccgctgc acgttttcaa
     6061 ccgtttgatt gaaaaatatc tcatcgcgca acgtcgcgcc tgtcaccgcc tccacctgag
     6121 gggcgaaatc ggcgtaatgt tgagtggaag cccaaatcat gaaaatcaaa tgctgcggat
     6181 caatcggcgc gagtttgccg cttttgaccc aaccggcaat cagcgcagat ttctcatcaa
     6241 ttaatgcctt caaatcgccc gtcagttcat ccattaacag cggcgcgcct gccagcatct
     6301 ccatacagaa caggcgcgaa gcctgcggat aatcgcggga gacttccagc ttcagacgga
     6361 tgtactcttt tatcgccgcc agcggagcga aatcttcacg aaacgctttt aacggtgcca
     6421 gccagatatc gagaatctgc cgcagcacgg caatatacag cgcctctttt gacggaaagt
     6481 aatacagcag attggttttt gaaacacccg ccaactctgc gatctgctcc agccttgtgc
     6541 cgtgaaaacc gaattgtgaa aaagtgtcca gtgctgcgct aagaatcgct tttttcttcg
     6601 cgcttactgc gcgcgaacgt ttacccgttg ttttcactgc gccttgcgtc atgcgctctc
     6661 ccctcttgta gattggcaac aggatagcaa aacccccgcc cggcctcgac cttctgcact
     6721 ctcatcgcgc acagatgcat gttttatgtg caactgtttt gaccgtttag tccacttttt
     6781 atcagatatt ttagataacg taatattaac atattaataa cattaacttt ttaaaaactg
     6841 gcatccgctt tgcaaacaag ccaatacgcg gctgaaaagg atatgcagga tgcagcgccc
     6901 cgtctgactt tcactttacg agacgaagag aggttgatga tgaaaattgg cgtattcgta
     6961 cctattggca acaacggctg gctcatttcg acccacgcgc cgcagtacat gccgaccttt
     7021 gaactgaata aagccattgt gcagaaagcg gagcattacc atttcgattt cgccctgtcg
     7081 atgatcaaac tgcgtggctt tggcggcaaa actgagttct gggatcacaa ccttgagtcg
     7141 ttcaccttga tggcgggtct ggcagccgtg acctcgcgca ttcagattta cgccactgct
     7201 gccaccttaa cgttacctcc ggcaatcgtc gcccgtatgg ccgcaaccat cgactccatc
     7261 tctggcgggc gttttggcgt caacctcgtg actggctggc aaaagcccgt gtatgagcag
     7321 atgggtatct ggcctggcga tgactatttc tcccgtcgtt acgactatct caccgaatat
     7381 gttcaggtgc tgcgcgacct gtggggcacg gggaaaagcg attt
//
LOCUS       DAZPJR010000197         7239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPJR010000197 DAZPJR010000000
VERSION     DAZPJR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.202"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..337
                     /locus_tag="ACHVB7_003852"
     CDS             <1..337
                     /locus_tag="ACHVB7_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1915721.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="ACHVB7_003853"
     CDS             337..666
                     /locus_tag="ACHVB7_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1915722.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="ACHVB7_003854"
     CDS             676..1374
                     /locus_tag="ACHVB7_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1915723.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="ACHVB7_003855"
     CDS             1379..2122
                     /locus_tag="ACHVB7_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1915724.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="ACHVB7_003856"
     CDS             2059..2661
                     /locus_tag="ACHVB7_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1915725.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="ACHVB7_003857"
     CDS             2722..6201
                     /locus_tag="ACHVB7_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1915726.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..6868
                     /locus_tag="ACHVB7_003858"
     CDS             6269..6868
                     /locus_tag="ACHVB7_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1915727.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6932..>7239
                     /locus_tag="ACHVB7_003859"
     CDS             6932..>7239
                     /locus_tag="ACHVB7_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1915728.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1637 a         1727 c         2315 g         1560 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cttttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgc
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 cagcgcgtat gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta
     6661 tctccgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatggcgg
     6721 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     6781 cgtggccatt gaccttgctt atgaaggttc cggcagtggt gactggcgca ctgacggttt
     6841 catcgtgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgactgcccg
     6901 ctggttcagg cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
     6961 agatggggcg ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag
     7021 tccgaccgtt gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt
     7081 gattgaggct gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac
     7141 ccgtgtcggg gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct
     7201 gaatgcacca aaggatggtg atttacgtcc ggaggtgat
//
LOCUS       DAZPJR010000198         7198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPJR010000198 DAZPJR010000000
VERSION     DAZPJR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.203"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..438
                     /locus_tag="ACHVB7_003860"
     CDS             <1..438
                     /locus_tag="ACHVB7_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1915729.1"
                     /translation="VICIIVRLSWAVNHYRDNAITYKEQRDKNARELKLANATITDMQ
                     QRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMG
                     NDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(527..1039)
                     /locus_tag="ACHVB7_003861"
     CDS             complement(527..1039)
                     /locus_tag="ACHVB7_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR1915730.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1234..1998
                     /locus_tag="ACHVB7_003862"
     CDS             1234..1998
                     /locus_tag="ACHVB7_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915731.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1949..3352
                     /locus_tag="ACHVB7_003863"
     CDS             1949..3352
                     /locus_tag="ACHVB7_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR1915732.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3352..4818
                     /locus_tag="ACHVB7_003864"
                     /pseudo
     CDS             3352..4818
                     /locus_tag="ACHVB7_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4709..5452
                     /locus_tag="ACHVB7_003865"
     CDS             4709..5452
                     /locus_tag="ACHVB7_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR1915733.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5456..6676
                     /locus_tag="ACHVB7_003866"
     CDS             5456..6676
                     /locus_tag="ACHVB7_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR1915734.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6680..7186
                     /locus_tag="ACHVB7_003867"
     CDS             6680..7186
                     /locus_tag="ACHVB7_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915735.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1915 a         1722 c         1964 g         1597 t
ORIGIN      
        1 gttatctgca tcatcgtccg cctgtcatgg gctgttaatc attaccgtga taacgccatt
       61 acctacaaag agcagcgcga caaaaacgca agagaactga agctggcgaa cgcaaccatt
      121 actgacatgc agcagcgcca gcgtgatgct gatgcactcg atgctaaata cacgaaggag
      181 ttagctgatg cgaaagctga aaatgatgct cttcggcgca agcttgataa tggtggtcgg
      241 gtgctcgtca aaggaaaatg ccctgtgcca tcctcagccg aaacctccag cgcctccggc
      301 atgggcaatg atgccaccgt cgaactctct ccagttgctg gacgaaacgt tctcggtatc
      361 cgggacggaa tcatcagcga ccagacagca ttgagaatgc ttcaggagta catcaggatc
      421 caatgccttg gggggtagcg gtaattttac tcattatcct tcaaatcaga ttctgttgtc
      481 agaggaatgg gggaagctgg attcgcaaga gctttttatg ggcagtttag ctaaaaaaag
      541 gcaacacgaa gctaaaacta ccaacataaa gtgctgacca gctagcagct gaaatcagac
      601 tcactgtata cactttcact ggatgaagtt gcaacattcc tgccaccaaa ggagcaatat
      661 aacgcagcac ggcgataaag cgcgaagtga aaagtattga cacagagtta ttttgcaact
      721 gaaggcgaac ccgttctatt gtattggaac ggcttgatat tatacatgca agttgaggga
      781 tatgcccaat tattatacca agatggtaat taacgatagt accgcaccat gcgccactca
      841 tgaccgtgat tccagcttcc catggtgaaa gagtcgtctt gctgactgta attacagtgg
      901 ccatcataat tgatgctggt ggcaatatcg ccgaaatgag taaagtggat tttgtgaacg
      961 ctattataaa aagaagcccc cataggcgat atggatgaag tacaaaatag ttcatcaaag
     1021 catttatcca ctccatcaat gtctttcctt ctgtgaattt ttctttgcta tttaaccgtg
     1081 atacgactta atttaggctt aaacacctta catttacaag gtactcctgt ggggggccct
     1141 gccacggagc gtcgcaggag tgagtgatga ggaagaatta aagcaaccta ttgtgacagg
     1201 cagcgataac agtaaatcac aggaagcata atcatggcaa aaccggactg ggaggccatc
     1261 gaatcggcat accgggccgg agtccttagc ctccgtgata taggcgagaa atacggcgtt
     1321 acagaagggg ctatcaggaa gagggccaaa aagcttggtt gggcacgcag tggcggtacg
     1381 caggtttgca aaaatggtac gcaaaaaagg aaagtgcgta ccagcagaaa gcctgccatt
     1441 actggcctta cacaaaaaag tacgcaacta aaaacagaat ctacaccgga tacgaaaccg
     1501 atacgcggaa tgcgtaccga tcccccgact aacccattcc aacccggtaa ccagcaggca
     1561 ttaaaacacg gtggttatgc ccgtcgcctt ctgctcaaag atgaggtgat agaggacgct
     1621 aaagcgttga cgctcgagga cgaattattt cgccttcgtg ctaacaacct tgtcgctgca
     1681 gagaatattg gtcggtggtt ggtgtcgctg gaagatgcta atggggacca ggaaaggaag
     1741 atgctgattg aaaatatcag cgccgccgag aaagcaatga tgcgcaatac agttcgtatt
     1801 gagtccatcg ttggcacgct tgcgacggta ggaaaaatat ttgctgatac agcctaccgc
     1861 aaggccgcca ctgataaggt gtctctggag gctgatcgtc ttcgccgtga tgcaggtatt
     1921 gatgatggca atggagagcg tgacctcaat gacttctact ctgacatcca aaccgacgct
     1981 gaatccggct ttacgtagtt tctggactat gcgggcacgt aacaaagtgc tttatggtgg
     2041 tcggtcatcg tcaaaatcat gggatgccgc tggcattgcc atatttctgt cgaataaata
     2101 caccctgcgt ttttgttgtg cccgtcagat ccagaataaa atcgaagagt cggtgtatac
     2161 cctgctcaaa attcagatag acaggtttgg tctgcggcac cgtttccgta ttctgaacaa
     2221 caaaatcatt aaccgggtta ctggctcgga atttgttttt tatggattat ggcgcaacat
     2281 cgaagaaatt aagtcactgg aggggatcga tgtgttgtgg ctggaagaag cccacgcact
     2341 gacggaatac cagtggaaaa ttctggagcc aacgatccgt aaagagggtt cggaatgctg
     2401 gttcatattc aaccccggac ttgttactga tttcgtctgg cgcaacttcg ttgttgatcc
     2461 gcccgaaggc actctcatcc gcaaaattaa ctatgacgaa aatccgtttc tgtctgacac
     2521 catgcttaag gttatcgacg cggcgcgacg ccgtgatccg gatggtttta aacatgtgta
     2581 tgagggcgtt ccggagtctg atgatgatgc ggcaatcatc aaactgtctt ggatagaagc
     2641 cgcagtggat gcgcacaaaa cgttaaattt cgaacccagt ggaagaaagc gtattggctt
     2701 tgacgtggct gacagtggta cagataagtg cgctaacgtt taccgtcacg gatccgttgt
     2761 tttctgggcc gacgaatgga aggccaaaga agatgaatta ctgaagagct gccagcgtac
     2821 ttatcaggcg gcgctggagc gtgaagcaga tattgtttac gactctatcg gtgttggtgc
     2881 gtctgccggt gctaaattct ctgaaattaa cgctgaccgg aagagcgaga acgcatacgc
     2941 gcgacgtgtg aattaccaga ggtttaacgc cggtgctggt gtgcatgagc cagatgacga
     3001 gtacaacggc atccccaaca aagacttttt cgcaaatctt aaggctcagg catggtggct
     3061 ggtggctgac cgtttcagaa atacgtttaa cgccattaac aacggagaac agtatcctgt
     3121 ggatgagctg atcagcatag attctcgttg tccgttgctt gaaaagctga aactggaact
     3181 gacaacacct catcgtgatt tcgaccgtaa cggacgtgtg atggtcgaaa gtaaaaaaga
     3241 cctcgcaaaa cgcgagatac cgtcaccaaa cgttgctgat gcattcatta tggccttcgc
     3301 gccaatcgat acatcgctgg atatctggga acagctgggg agacaggcct gatggcacga
     3361 aacaaacaag ccctgcggcg aactgtgcag gccacagctg atggttatga gaattttatt
     3421 gcccgcgtag ggatgcagac acctaaccag cactcagcat ccacctaccg ggctaatttc
     3481 accagtcgta accgcatgct ggtggaatgg tcctatcgtt catcctagat catcggcgaa
     3541 gcagtcgatg ctatcccgga tgatatgacc cgcaaaggca ttcgcatcac ttcggaaatt
     3601 gatgcaaaag atcgtggcat tctcgaatca caactggatg agttgcaaat ctgggatgcg
     3661 ctgaatgacg tgctgaaatg gtcgcgcctc tacggcggcg cggtgggttt catcatgatt
     3721 gaggggtagg caccaatgac cccgctgcga cccgaaacca tcggtaaggg caagtttaag
     3781 gggattctcc cgctcgaccg ctggatggtc gacccggcac tgacccgccg cattaaagat
     3841 atggggccgg acctgggtaa acctgagttt tacgatgtgg tgaccacagc aacgggaatt
     3901 cctgcctggc gcattcatca cagtcgcctg attcgctttg atggcgtcac gttgccattt
     3961 cagcagaaga tgaccgagaa cgaatgggga atgtcggttg tagagcgtat ctgggatcgt
     4021 cttaccgcgt tcgacagcgc tactgtcggc gcggcgcagc tggtctacaa ggcgcatttg
     4081 cgtacctaca gcgtggagaa gctacgcgag cttatcgcac ttggtggtcc tgcgtatgaa
     4141 gcgttgctga agaatatcga cctgattcga cagttccaga gtaatgaagg catgacgctc
     4201 atggactcgc gggataagtt tgaaacccat cagtacagct tcagtggtct ggatgacatc
     4261 ctttcgcagt ttgtagaaca gattagtggc gctgttggta tcccactggt gcggttgttc
     4321 ggacagtccc cgaaaggatt ttctaccggt gatgcagacc ttgccaacta ttacgatcgc
     4381 atcagttcgt tgcaggagag gcgtttacgt cttccggtgc ggcggatact ggacatcatg
     4441 catcgttcgg aacttggcaa gccgctgccg gacgatttca cgtttgagtt taacccgctc
     4501 tggcaaatgt ctgatgtcga tcgctcaacg gtggcgttaa acaccaccaa cgcaatcagt
     4561 acggcgctgg gtgatggtct gatgacactg aaagccgcta tgactgattt gcgagaaaat
     4621 tctgacgtaa ccggcatcgg ggcatccatt accgacgagg acatcgagaa tgccgaagat
     4681 gaagcgccgc ccggcatcgg cgaacctgat gacgaaccgc aggagccgtc aggcggaaat
     4741 ccggtatcga accagcctac gcaggatagc gagggcggtc ggagacatcg taaatggtcg
     4801 ctacgatggt tcaaatgaca gtatcacgga aattattgcg gcgctggagc gttacagtga
     4861 aatcatcacc ccctgggcga caaaggtcgc ggaaaacttt actgcggacc taacccggca
     4921 gaacgagaaa gtttggcggc aacacagcaa gaacatcagt cgcgagctcc gcaatcttgt
     4981 ggaaagcgct cctgtgggcc aggtgatgca atccatcatc gccgaacagg tcaagtacat
     5041 caaatctctg cctctcgagg ccgcagacag ggtgtacgac atccagaaca aagcgataga
     5101 ggctgttgtc accggtggtc gggcggagca gtttgctaaa gagattgcat ctaccggcga
     5161 tgttgctaag tctagggccg atctgattgc ccgaacggaa ctgggaagag caacgggcgc
     5221 gctggatatg gcccgagcga tagctattgg ctcggatggt tatatttggc gtacagccga
     5281 tgatggcgat gtccgagatt cccacgatca catgaaaggt aaattcgtcc gctgggattc
     5341 acctccaact ttggacggca tgaccggcca cgcgggcgag ctgccaaact gccgctgcta
     5401 taaagagatc gtgtttgttc gcgttccatt cgcaatgaaa agggcagcat aacccatgaa
     5461 atactttttt gagaccaggc tcggggaaac ccgataccgc ctggctgacg gctcgttgct
     5521 gtgcaaagac gtgccgatag gacgaacagg taagcagctc tatggtgctg atgacctgcc
     5581 aaaactgaaa cccgataagt tcggtgaaat agtcgtcacg cgttctcctg agcaggtatt
     5641 ccatccggcc acgcttgcct cattcgaagg gatgagcatc acgatcctgc atcctgaaga
     5701 tgaaaacggg aatgtgcggc tggtaaatcc cgagaactgg aaagagcttg ctgtcgggca
     5761 cctccagaat gtccggcgcg ggacgggtga gcagtctgat ttgatgctgg ctgaccttat
     5821 cgtcaaagac gaaagcgcca ttcagcttat cgaagatggc ctgcgcgaag tgtcgtgcgg
     5881 ctatgacgcg gagtacgagc agaccgagcc aggtaaagcc gagcaggtcg atattaccgg
     5941 aaaccatgtg gctcttgtcc ctaaaggcag agccggaaat cgttgtgcaa ttggagacag
     6001 agacacaatg gcaaatcaaa agaaaaactg gtggaaccgc atgcgtgcgg ccatcaagac
     6061 aggagatgcc gacaccatga acgaactggt ggagtcggct cccgcatcgg ttacaggaga
     6121 tgagggggat ttgccgcagg gcgttaatct caacatcaac ctgtccccgc agcaaccact
     6181 accggacaaa gcaccagaga tgggcggaga tccaaccggc gacagtgatg atgacctcaa
     6241 aacattactg aaagccctgc tggctaagct ggaaagaaat gccacgggcg ataacgataa
     6301 taagcctgac gataatccga ccggtgacgg cgaggacgat gaagaggaaa ccacgattac
     6361 tggtgactca gcctggcgtg ccgaagttat cgttccgggt atcgatctga gccgtaagat
     6421 gaaaccgacc gcgttcaaac gcgaggttct ggcttctgct gacaaaacgc tggttcgcca
     6481 gatagtcggt gatgcggata tccgcaaatt accgaaacaa tcggtcgaca tggcgtttaa
     6541 tgccgtgtct gaggttgcca aagggcgaaa cacccgcgcc accaccggcg atgcacagcg
     6601 cctaaacatg ggcatgacca gtatcgcttc cctgaacaaa caaaacgctg aattctgggc
     6661 aaaccgtaaa gggtaaaaaa tgaataatgt atttctgtac cggatgcctg ttggcattgc
     6721 cggggctgtc tctcgcccgc aggacttaac cgtcgaaccg gtggtcctta aatccgataa
     6781 cgccttcgct gcctatggcc tggctggtaa atacgatgat gacggttttt tcgtgccgct
     6841 ggcagatggt gataccgcag acaaggtgaa ggggatctac gtgcgccctt atccgaccac
     6901 gtcgcagccg gacatggttc gccaggtggg aacaggcaag aacttcccgg gcgacgccat
     6961 gaagcgtggc tacgtgaccg ttaatctcgg ttctgatttt gatgccagca ctatcaaaaa
     7021 aggcgatccg gtatacgttg tcgtctccac tgatgaatcc atcaaagtgc cgctgggtgg
     7081 attcatgtcc acgtcagtca gtggcaaaaa cgtggtgctg accaacgctg aattcacagg
     7141 tgccggtgat gctgacggca atgcagaaat ttcctggaag atttaaggaa cagacgaa
//
LOCUS       DAZPJR010000199         7122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPJR010000199 DAZPJR010000000
VERSION     DAZPJR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.204"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..436
                     /locus_tag="ACHVB7_003868"
     CDS             <1..436
                     /locus_tag="ACHVB7_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR1915736.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            519..1421
                     /locus_tag="ACHVB7_003869"
     CDS             519..1421
                     /locus_tag="ACHVB7_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR1915737.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHVB7_003870"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHVB7_003870"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR1915738.1"
                     /translation="MDVSYLLDSLNDKQRETVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHVB7_003871"
     CDS             complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHVB7_003871"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR1915739.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHVB7_003872"
     CDS             complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHVB7_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR1915740.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5331..6038)
                     /locus_tag="ACHVB7_003873"
     CDS             complement(5331..6038)
                     /locus_tag="ACHVB7_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR1915741.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHVB7_003874"
     CDS             complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHVB7_003874"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR1915742.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6896..7099)
                     /locus_tag="ACHVB7_003875"
     CDS             complement(6896..7099)
                     /locus_tag="ACHVB7_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1915743.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1640 a         1990 c         1854 g         1638 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcgacat cgttcgtctg gatgccttca aaaccagtaa
      121 agagattcag tttgcggtgc agtcagggcc aatgttgatg gaaaacagtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagaggaaa ggttaagcat tgtacactcg aggaggccag acaaactggg gctggctaca
      481 cagctgaaaa atcatcagat aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacgt
      541 tatttttatt aacgtgtctg ggatcatcat ccgcttatgc ggataacgct ttgattttac
      601 aaactgattt tagcctgaaa gatggtgccg tctcggcgat gaaaggggtc gcattcggtg
      661 ttgaccataa tcttaaaata tttgatttaa cacacgaaat tccaccgtat aacatctggg
      721 aaggcgctta ccgcctgtat cagaccgcca gttactggcc gcaaggttcg gtatttgtca
      781 gtgtggtcga tcctggtgtt ggtacagacc gtaaatcggt agtgctaaaa accaaaaacg
      841 gtcaatactt cgtttcacca gataacggta cgctcacttt ggtggcggaa tcgctgggta
      901 ttgagagcgt ccggcaaatc gatgaaaaaa ccaaccgcct gaaaggttcc gaaaagtcat
      961 ataccttcca cggtcgtgac gtgtatgcct acaccggtgc acgcctggct tccggagcga
     1021 tcacgttcga acaggtgggg ccagagcttc ctgcaaaagt tgtcgaactt tcttatcaga
     1081 aagccaaagc aacaaaaggt gaagtgaagg gcaatatccc gattctcgat attcagtacg
     1141 gcaacgtctg gagcaatatc agcgacgagt tgctgaatca ggcaggaatc aaactcaacg
     1201 acacgttgtg tgtaaccatt tccgaaggtt cccagcaaaa atacgcaggg aaaatgccgt
     1261 atgtcgcaag cttcggcgat gtgccggaag gccagccgat ggtgtatcta aacagcttat
     1321 tgaatgtttc cgtggcgctc aatatggata atttcgcgca gaaacatcaa gttgcgtctg
     1381 gtgctgactg gaatattgat gtgaagaagt gcgctaagta aggtgctgat gcaggatgcg
     1441 gtgtggcgca ccgcatcctg caacggagtt gtaaattaca ccgtctccag ccgggcgtaa
     1501 gccgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg gctatgttca
     1561 ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg cacgcgctgg
     1621 ccgagtttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg atggctaacc
     1681 gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg cagctcgccg
     1741 ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt ttccgcgtag
     1801 gtcagcgtca gtttctgcat cgcacgggtt acgccaacgt aggccagacg gcgttcttct
     1861 tccagacgtc cgccttcatc cagcgacatc tggcttggga acatgccctc ttccataccg
     1921 acgataaaca cctgcgggaa ctccagccct ttcgccgagt gtagcgtcat caactgcacc
     1981 gcatcctgcc aagtatccgc ctgcccttca cctgcttcca gtgccgcatg ggagaggaac
     2041 gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg cgttgccgtc
     2101 accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc ctgctcgtac
     2161 atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg
     2221 gcagtttctt gcgctaaggc atcgattaat tccataaatc gctgcaaggc gctggcagct
     2281 cgcccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg
     2341 cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc
     2401 gtattcacca cgcgctcaaa cgccgcgtcg tcgttacggt tggcaatcag acgcagatac
     2461 gagagcgcgt ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacggtac
     2521 ggcatactgg cctgcaacag tgcttcttcc agtacacgcg actgggcgtt actgcggtag
     2581 agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aagttttgat gcggttaacc
     2641 acaaaacgcg cttcgtcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc
     2701 gcgccatcgg tccacagttt tttacccaga cgcccgttgt tgttttcaat cagggcgttc
     2761 gcggcgctca gaatattgct ggtagagcgg tagttctgct ccagacggat agtttcagca
     2821 ccggggaaat cattgaggaa acgctgaata ttctctacct gcgccccgcg ccagccgtag
     2881 attgactggt cgtcatcgcc aacgatcatc actttgccgg tatcgcccgc cagcaggcgg
     2941 atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat gttggtaaaa
     3001 cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc acaactcgtg agcgcgcagc
     3061 agcagctcgg cgaagtccac caggcccgca cgatcgcacg cttcctgata cgcctgatac
     3121 accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg cggacgcagg
     3181 ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg
     3241 tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc actgtcgagg
     3301 atctggaaat cctgcggtag attggcgtcc atatggtgcg cacgcagcaa acggtgcgcc
     3361 agcccgtgga aggtgccgac ccacatgccg ccctggctgg tgcccatcag ttgcccgata
     3421 cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat cgagtatggc
     3481 gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca
     3541 ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgt ttcgcgctgt
     3601 ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag
     3661 gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttactg
     3721 attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc
     3781 tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc tgcattccgc
     3841 tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag atctcgccga
     3901 tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt tttgagcgcc
     3961 catgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac agttccggct
     4021 gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt
     4081 cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta atcattgctg
     4141 cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa
     4201 tcgaacgaaa acgccaacgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg
     4261 cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt
     4321 gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg
     4381 tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta
     4441 tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc
     4501 aagatgagta tagatttgcg tggtggagag gttggcatga cccagcagct cctgcacacc
     4561 acgaagatcg ccgctcgact ccagcatatg cgtggcgaac gagtgacgta atttatgcgg
     4621 atgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt
     4681 acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt cgctaccaaa
     4741 caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac caatcggcag
     4801 gcggcgctct ttgctgcctt tccccattac ccacacttca ccagactcca ggtcgaggtg
     4861 tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca tcacttccag
     4921 cattgcacgg tcgcgtacag cgaggggatc gttgatatca atgtccagca gccgattcat
     4981 atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg aaacaccttt
     5041 cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc
     5101 agaaagccgt aacgcgaggc ttgctgctcc cagcccttta cggcgactgc gtacagcaaa
     5161 attgcgcacc atcgtcacat cacattgctg ccagctttgc aggccgtttt cgctggcaaa
     5221 attgatgatc gcctcaagct gacgctggta gttaagcagg gttatcgggc taagctggcg
     5281 ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg tcatacgcgt
     5341 tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag taactgcgtt
     5401 ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacgac acccaaatca
     5461 gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc
     5521 accagcagct ctggtccgtt aagcggccca agatagtgct gttcctgccc caaacgctga
     5581 atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca
     5641 cctaagcgcc agcgatccgg aaacaggcgc agactcgcac ctgccaggcc gagatcgcgc
     5701 gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt gagactgcgc
     5761 tgcaggtaga gtaggcgata aaacaggcct tcgttggcga tagcctgttc catcaacagc
     5821 gccatgttct cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc
     5881 aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc gcgcgcatta
     5941 cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc
     6001 gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga
     6061 catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa
     6121 gacgaccgcc ggggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta
     6181 ctgcaaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat
     6241 aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggcgcgct
     6301 ccggaaaacg ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga
     6361 catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg
     6421 tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacgg
     6481 cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg
     6541 tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg gtcagtcctt
     6601 tcagacgcac aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc acttcactgc
     6661 catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca
     6721 ccagcagttg gtcaaaccct acccccaggt gccgatcagc caggcgacga atcagctccg
     6781 gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat
     6841 gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt
     6901 aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc accgtggatt
     6961 gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca ggcgggaaat
     7021 agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat acagtgagtg
     7081 ccttaaacac gtttttcatt atggattgcc tgtaaagttc at
//
LOCUS       DAZPJR010000200         6847 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPJR010000200 DAZPJR010000000
VERSION     DAZPJR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.205"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..213)
                     /gene="cas6e"
                     /locus_tag="ACHVB7_003876"
     CDS             complement(<1..213)
                     /gene="cas6e"
                     /locus_tag="ACHVB7_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR1915744.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(138..884)
                     /gene="cas5e"
                     /locus_tag="ACHVB7_003877"
     CDS             complement(138..884)
                     /gene="cas5e"
                     /locus_tag="ACHVB7_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR1915745.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="ACHVB7_003878"
     CDS             complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="ACHVB7_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR1915746.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1962..2498)
                     /gene="casB"
                     /locus_tag="ACHVB7_003879"
                     /gene_synonym="cse2"
     CDS             complement(1962..2498)
                     /gene="casB"
                     /locus_tag="ACHVB7_003879"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR1915747.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2495..4056)
                     /gene="casA"
                     /locus_tag="ACHVB7_003880"
                     /gene_synonym="cse1"
                     /pseudo
     CDS             complement(2495..4056)
                     /gene="casA"
                     /locus_tag="ACHVB7_003880"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(4154..>6847)
                     /gene="cas3"
                     /locus_tag="ACHVB7_003881"
     CDS             complement(4154..>6847)
                     /gene="cas3"
                     /locus_tag="ACHVB7_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR1915748.1"
                     /translation="KYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVA
                     AMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPEL
                     KIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGH
                     HGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYI
                     SATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINT
                     LFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGL
                     FFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGS
                     EEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKIL
                     LADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQE
                     APFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGK
                     CIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDS
                     SQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGK
                     DERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPH
                     AARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLP
                     EKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEG
                     EAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1660 a         1861 c         1620 g         1706 t
ORIGIN      
        1 gagttcttcc cgacgataaa gaaattgcct ttctttgccg ccagggaaaa gatcccacag
       61 ccactgatgc atcacatatt caccgcgatc caccagatgc agcaactgcg caggcgaaag
      121 ctggccggta tgtaaggtta ttcttgagag gtacatggtt cctccttgct gagccacggc
      181 ccctgattga cggtgcgttc cccaaacagc cactgctgac gatttaaagg aacatcccgg
      241 cggcgtaata ttttgctcgc gaccaggccg tcgtgtttcc cttcccacca gcattcatcc
      301 tgaagtttcg ggagtgagac tttcagttta tggaaatgat cctgatactg ttggtatgca
      361 ttacataagg catcacacgc gttgccttcg agcagtaacg gcgcaagagg taatgccagc
      421 ggatgacttt ttcgccccag ataaagcgga aaaactggat ggcgtaaacc gtcctgcaac
      481 tgttcgaggc tgtaaggcgc atcggctgtt gttgccaccg ccaccatcca ccaggcatcg
      541 gtgtagtagt cgcgccggga gataatcgcg ctcaaaagct cagggtcgct caactcttca
      601 cgacggctga aataacgtgc tttacgcacc tcttttggca tctggaccgt gtgataatcc
      661 cgtgcccagc gcgggttacg gctggcgcaa accaccagtg aatagtggcg attaaacgcg
      721 tttaagcgtt cggtatcatc acgccgaatc cctaccccgg cagccagtag ccccagcaat
      781 gctgagcgcg aaggaagttc atgggtatga cgaacttcac ctggagcatc gacaccccag
      841 gacgccatcg gcccatgaag ctgaaaaatc agatattggc tcataagccg ccccttacgc
      901 gcagataaag tccagcacgt ccttcatgct tccctgtttg ttcatcacgt caaaacttgc
      961 gcattcggtc ttctgttcgt agaccgtatt catgttttcg cgaagcgttg taatacgctg
     1021 caccgccaca tctaactgac gtgtgccatt aatgggttca tagaaagccg ccgccagaga
     1081 gcgtggttga tcggtgcctt tttctgccag cgcccaggaa gcataggcac ggctggcaaa
     1141 gctattttgt ttaccggttg gggaaacttt aagcgcggct tccgtaaaag cgcgtatggt
     1201 ctgattagct aacgcttcgt caccgccgag gttttctacc agcagatctt tatcaatgca
     1261 gatataggtg tagaaaagtg ctgaaccgaa tccggtttct ccgagatgcc cggcaccggc
     1321 atcttcagag gcctggcgca aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt
     1381 ttcactgaca ccaaacgcat gcgcgacctg gcaggcggct tcaacattaa actcaggttt
     1441 attcgccagc atacgaccaa acatagcgat atctaccgct atacggtctt tacgtaacaa
     1501 cgcgagatct tcctcttttg gcgcgcgctt ttcttctgcc agttgatgag caagcgcttt
     1561 tacggcgtca aattctgccg ggctgatatg aactaattgt tcagtttcgg cgttagtgag
     1621 cggatctttt ggttttttgt catttttagc tttcccaaga taatcagcaa tttttgccgc
     1681 ccattcgatg gcttttttat cttcaatccc tttctcaatc aggatagttg ccgcctcacg
     1741 cgcaatacgc ccactgcgaa taccaatatg gcccgccagt gcctgttcaa aaagtgcaga
     1801 agtgcgccac gcacgtttca gactttgcga ggaaacgcgc agacgcgttg ctccacccag
     1861 caccacagtt tttggcgcac cggtgtcatc acggttaagg ttggcagcag ggtaagcggt
     1921 taacacatga agctgaataa atgttgtcat gagatagtcc tttattgatt ttgttcgtta
     1981 tcggcgtcgc cagcctggta atattccagc gcccagcgga tacggataaa ttcggttggc
     2041 cgctgctggc ggcggtgatg attgagcagg tcgtcgctct cctggcacca gcggaaaatg
     2101 ccatctgcca gggagtcaag attgacggaa ccgtttaata atctgacggc gcggcgtaac
     2161 tggcgcagta attcatccgg tgtttttact gctgacaggc gagtaaaacg cccctttgac
     2221 attaccgccg ccagttgcgc ggcaaaaggc tgccgttcat caatggcttt aacattggcg
     2281 ctaactgcgg ctatcagcgc cagtgcggtg atacgccact caggttcatc ctgccacttt
     2341 atttgtctgt tcttcagaaa caggcgaaat ccatccgtca gacaaacatc attaaccgtc
     2401 gtactacgcc gcagactggc acgttcgccg cgtttctcct gcaattcctc gtgccatttg
     2461 cgcagcgtgg ctttgtgctc ctcttttaca atactcattc agcagcctcc tgctttttct
     2521 ccctggcggc tttagcactt tgcttctccg ccgatgttgt aaaatatttc ttgcgcgcgg
     2581 tcatgatgcg ttccaaatca acgggctcat aaggattggt gaatacacgc tcgtcaaaat
     2641 cctgacgcgc gaataaccaa atttccttat tccatttgct gagtaattca tccgcatcct
     2701 gaccttcttc aatttggcgc actaacctca ggaagcgatg ctgagttttg ttccagaagt
     2761 cgatatccac aaaactaaaa tcaccccttg caccttttgg atcggagaac catgcttctt
     2821 tcaatgcact ccgtaacaga cttagaatcc gtgaagccgt ttgcgcagcc agccgcagtt
     2881 tcggtatctg gccttctttt tattgagcag cagcgggaaa tgatgttcgt accaacagcg
     2941 cgctttcatg ttgtcgaaat catagccaaa tccccacagg cccacttttg cctgcttgag
     3001 actgctggca ttaaagagtt tcaccaccag cgcgggaagt tccgtattgt tttctgactt
     3061 acccgtttcg ataaggccta accagtcgcg ccagattaaa ccgcccggtt gtggtttgac
     3121 cgagtaaaac tcgccgccct ctttaagtag tacacggtaa ggcgttaagg gatgctgcca
     3181 catggcatag ttagcgccgt aatttttggt cgtcatcagg ctcagaagcg cgtcgctctg
     3241 ctcaccgcaa atatcgcagt tgccgactgt ggtggtatta aaatcaatac gaatacggcg
     3301 aggcattccc cagtacgcct ggagtttatt gacctgctca tcggttacca ccgcaccggc
     3361 cagttcgctg atgcgcgtcg ggccaagcca ggggaaaacc agatcgtcaa attttttggg
     3421 tagcggtaaa tcggcttcat cctgcggcat gacattgatc cacagtttgc gccacaaggg
     3481 agtttgttga ttaccctgat attcctgtaa ttcaatcaga gtggtcatcg gcccaccgcc
     3541 gcgtaaaccg gtgcggtagc ctttgccacc tgacggcgca ttcaactgta gggagaacag
     3601 agctaacgca gagcaatgtg ggcacaggta ttccgttaca ccacgcttga taaagtggtc
     3661 tttattaaac ttcgttgttt gagcgccggg aatctcaggc agtagcgaag cgaccggaac
     3721 tttatctccg gtgagcgcct cgaaatcctg tataaatgaa ggggaatctg gcccaaactg
     3781 gaaagcatgt tctaatgaca gcaatgcttc ccgtagcttt tcagcttcca gtccgtcttc
     3841 ccagatatca tcccaacgac gatgatcttt tggcgcgaag ctggtttgta gtagccccag
     3901 caaaaactgc cataccgcac cctggagatc tgcccgcggc gcagaaatat cgacaacatt
     3961 ttcatccgcc agatcgactg gcgccagttt gcctgtcgtt ccgtctttaa aacgaacggg
     4021 caaccacggg gttgtcagaa gtgaaaacga gttcatacgc atcctctcgt aaaaaagacc
     4081 atcccaggtc ggttgtccat ctttccatcc ttttcggtgt atcaactatt cagagagtcc
     4141 tgtcgcagac atctcaacca accttgccaa tcaatccttc cgtagccgaa tacccacaac
     4201 tttcgtcatc gttgactaaa ataacgtttg ccatctccgg atcttgtcgc tgttcaatgc
     4261 accacagcct gaacgcctct ccctccagta aagaaaactc atcccgatgt tttttccacc
     4321 agcttcggcg cactctgaca acgctcattt cccatgcgtg agcaccggtg gcataaggct
     4381 tcaccacacc gtcaatacag gtagccagcc acagggaaac agactcctca gccagacgtg
     4441 tcgacagttt ttctgggagg taatcgttga tattggcggc atagccgggc ctgaagttca
     4501 gcacaaactt tttagccatt gcgcgatcac agtaatattt gcccacttgc tcctgctcgc
     4561 tgcgggcaaa tccttccggc attaccacgt cctcaccgta gacggattca ataagaagcc
     4621 gggctgcgtg tggcatctga atagcacctt gctcacgcag cacacgctgc gtcagccaga
     4681 ttcgcccatg atcgggatag acaaatgcac tgttacgcat ggcactgcca aaccattcgt
     4741 cgccaggaga gtcgtcccag acgggagcca gaatcagcaa ttcagggggg gaacgctcgt
     4801 cctttccgtc acgctttaac tgaccattaa tatcgcggat atgccgctgt aatcgtcctg
     4861 ctcgctgaat cagcaaatca acaggggcca ggtcggagat catttcgtcc aggtcacaat
     4921 cgacactctg ctctaatacc tgagtacaaa taaggacttt tccggcacgc tgtgagctat
     4981 cttctttacc aaagcgtgcc agtgtctccg tttcaattcg ctggcgatcg ctaaaagcaa
     5041 agcggctatg aaaaagtgaa aggctggaag caggaatgac gccgcgggca agcagctgac
     5101 gatgaacctt aatagcgtca tcgacagaat tccggatcca ggcgatgcat ttcccctgac
     5161 ttaccgccga ttcgatacgc gcaatacact cttgttcact atgaagccaa cccacgctga
     5221 cgctacgctc aacgtctttg cgcgtcgcta cccggtgtga gttcacatcg gatttcgtga
     5281 catgcgtcag ccaggggtaa tcatcctttt ccaggaacgg agcttcttgc aggccctctg
     5341 taccccgcgc aaaggcagcg acgagtttgt cgcgctgctg ttgagataac gtggcagaaa
     5401 gcaaaataac gctgtttccg ccacgcgcct gccgctcgat caacccttca agaatgcacg
     5461 acatgtaagc gtcacaggcg tggatctcat cagccagcag gattttgtta ctcagcccca
     5521 gaagccgcag attattatgt ttgaacggca tcaccgccat catcgcctga tccagcgtgc
     5581 cgacgccaat ttcagccagc aacgccttct tgttactgtc ggcaaaccag gccgcacatc
     5641 cctgactgaa cgtttgttca tccggttctt ctgacccgac taaatcacca gaccagagtg
     5701 attcattaaa gcggtccatt aatgtgcggg cactatgtgc cagcaccagg ctggggcggg
     5761 attctggcga atagaaagca agccaggttt tgaccagccg atcgtacatg gcattggccg
     5821 ttgccattgt tggcagacca aaaaacagcc cctgagcctt ccccgcagct atcaacctgt
     5881 gcgccaggat aagcgccgct tctgttttac ctgcgccagt cacatcttcc agaataaata
     5941 actgcggtcc ttgctggctg atatccagat ccagcacctt ttgctgtaac ggtgtcggat
     6001 tctcaataaa aggaaacagc gtattaattc cgttgaaagg cgcgactttt gcttttagag
     6061 gaaagacggt taaggcgttt tgagcctgaa ctaaagtttt ctgccagtaa cctttaatat
     6121 ccattgggtg tgcgacgcgt ggaaaaaatc gcgttgacga acccgtccag tctgcgagca
     6181 cgacggttgc agagatatac caggaaaggt gttttataag ttcaatgccc tcatcatcat
     6241 cccagaatgc ggggatctct atgagcggaa acagtggttt gatttcaagg agaaaatctc
     6301 gtgctgcagc tttgtcttca ggcagaaaat tatccaattc atccatacga tcagggggcc
     6361 aaccatgatg ccctgtcgtc acgggcatcc acatttctat cactcgccta agtttgcgcg
     6421 gagagagcga agatgaagga agtaactcct gacattcact taaataatga ttccacaacc
     6481 agtagcccag cgttgaatga gagatttttt cgtaattttt tctggcacct tcctgaatct
     6541 tgagttcagg gggcaggtaa agttgttgaa aagagcgggc aaattttcca atatcgtgcc
     6601 agcacagcaa ccaggcgaaa aattgagccg cctgttcctt gtcagaaatc cctaattgac
     6661 gaaaataatc agccagcccg aagcaatttc ttttaaccat taaatagccc atcgcggcta
     6721 catccagcga atgccagcaa agaaggtgat agtcgtcgcc cccgtctttc tcgccgcgtc
     6781 gggtttttcc ccagaaatca aaaaaagtca caatattttt atccttcagt aaacttaaag
     6841 gatattt
//
LOCUS       DAZPJR010000201         6748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPJR010000201 DAZPJR010000000
VERSION     DAZPJR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.206"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(197..682)
                     /gene="sixA"
                     /locus_tag="ACHVB7_003882"
     CDS             complement(197..682)
                     /gene="sixA"
                     /locus_tag="ACHVB7_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR1915749.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(885..3029)
                     /gene="fadJ"
                     /locus_tag="ACHVB7_003883"
     CDS             complement(885..3029)
                     /gene="fadJ"
                     /locus_tag="ACHVB7_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR1915750.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3029..4339)
                     /gene="fadI"
                     /locus_tag="ACHVB7_003884"
     CDS             complement(3029..4339)
                     /gene="fadI"
                     /locus_tag="ACHVB7_003884"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR1915751.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4519..4803)
                     /locus_tag="ACHVB7_003885"
     CDS             complement(4519..4803)
                     /locus_tag="ACHVB7_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR1915752.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5175..6515
                     /gene="fadL"
                     /locus_tag="ACHVB7_003886"
     CDS             5175..6515
                     /gene="fadL"
                     /locus_tag="ACHVB7_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR1915753.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1675 a         1813 c         1615 g         1645 t
ORIGIN      
        1 agacattatt catttcaaca tgtaactttg ggcaaaatca agatacgcgc agaagaataa
       61 tttccgaatg aaagtagaaa gtgttttatt tatagatggg gagagtcttc gatagtagtg
      121 agattgacag atatccgctg cctgatgagc aattatgccc aatgaatcta cctcattggg
      181 cataatttgg aactcatcag atagcttttg ccatcttcag attacacggg ctcatctgcc
      241 agttgaatgt gccgtttccg ctttcatcaa gcgttacgct ggcaatggcc gaggtggtaa
      301 acataggcgg cgtttcgccc ggacataact cggcaaccag atacccgact aacggtaagt
      361 gagagatcac cagtaccgaa gcaacacctt cattggtcaa cgcctgcaaa taggcactga
      421 ccagaccaac atcgccgcag ggcgttaact ccggcagaac ttctgcactg gaaggcaggt
      481 tcagacaatc ccctacttct tccagtgttt gctcggctcg caggaacggg ctcaccagaa
      541 cgcgttcgat ttccactttt tgacctttca gccagttcgc catcaggcga gattcgtcac
      601 aaccattagt ggtcagagga cgaaccgaat cactggcggc atcaagggct gcgtcgccgt
      661 gacgcatgat aaaaacttgc atattgcacc gcttttgtta accagattcg tccgttttct
      721 ttcatctgaa accagagaaa gaaaacggtg gccggcattg tgccttatcc attcaccgaa
      781 tgaaacgctg ttttttacct caatggcgta agtatagtca atatgcgttt acattttgac
      841 caacactccg ccattcagcg cggattcata tagctttgac cttcttattg aaggtcagtt
      901 gcagttgttt tccaaaaact ttccccacgc gcgcccatct cgaccaaacg ctcgcaaggg
      961 gtaaaacggg aaccatactg cgtggccagt cgttgcatta ttgcaaccac ttcgcccgcg
     1021 ccgagagaat cgatatagcg gaacggtcca ccgagaaatg gcggaaaacc aatgccaaat
     1081 accgcgccaa tatccccgtc acgcacgcta cggataacct gctcatcaac acaacgtact
     1141 gcttcattca gcatcaacat cacacaccgt tcagcaacct gcggtgcgga gagtcgcccc
     1201 tgcccttgtg cgccaatgag cgggtaaatg gcgggatcga cctgtttttt gcttttacgc
     1261 cctttctgac cataaagata gaaaccccga ccattttttc tgcctttgcg atcgtcgttc
     1321 aaaattgaag aaacaacatt tgcaggcgcg ctaaaacgtt ctccataagc ggcttccagt
     1381 acaggaataa ttttagtccc ggtgtcgatt cctacctcat ccaaaagttg gattgggcct
     1441 accggaaaac caaatttcac tagcgcggca tcaatgtgct ctacccgttc accttcggtc
     1501 agcatgcgga tagcttcatt aatgtaaggc gctaagatgc gattgacgta aaacccggct
     1561 ttgtcacgca cgacaatggg cgttttgccc tgttttttcg ccagttttac cgtagtggcg
     1621 atggtttgcg ccgatgtacc cgcatgagga ataatctcta ccagcggcat tttttccaca
     1681 ggactgaaga aatgcaggcc aataacttgc tcaggtcgcg cggcgtgagc ggcgatatca
     1741 ccaatcggta aagatgacgt gttcgaagca aaaatggtat gagcggcgca attttgctcc
     1801 acttccgcca ccatctgttg ttttaattcg agattttcaa acaccgcttc aataatcaga
     1861 tcgcgatggg caaagccgcg atagtccgtc gttccggaga ttaatgccag ctgtttgtca
     1921 cgttcgctgg ctttgagatg acgacggcga actttgccct ccagctgatc ccaactgtac
     1981 ttcagcgcat gatttatgcc ctgcgggttg atatctttaa ttctgaccgg aagccccgct
     2041 ttacaagcag tgacataagc aataccgccg cccatcaagc caccacctaa aattcccacg
     2101 ctgtttaatg gcgcaggcgg tgcatcacta ccgggatctt tcttcacgtc cgtactggca
     2161 aaaaagatac tacgcaaagc ttgcgattgc ggcgtcatcg ccagttcgcc aaaggcgcga
     2221 gcttcagcgt catagccgct gctggtgccc tgcgctaatc ccgtttcaac aacctccagg
     2281 atgcgttctg tcgccggata attgccttga gttttgtgtt ctgttttctt gccgaccatt
     2341 ttgaacagca gcgcacgacc taacggcccc gccagaatac gctcgcgtac aggtagaggg
     2401 cgggaagatg ggcgatcctt cttcgccagc tcaacagcgg cttccagcaa aatggagtgc
     2461 ggaacaacgt catccaccag ccccagcttt aatgcctgtt tcgcccgaag ttgttttccg
     2521 gtgagaatca tctctaatgc tgtactgacg cctatcagac gcggtaaacg ctgggtgccg
     2581 cctgaaccgg gtaacaatcc aagttgtact tcaggcaaac cgagcaccgt tttaggatcg
     2641 tcagtacaaa cgcgaccgtg gcacgccagc gccagctcca gcccaccgcc caggcaagcg
     2701 ccatgaatag ccgcgataac cggaatgggc aaagcatgaa tctccgccat caactgttgg
     2761 ccctgccgcg ccagtgcttc cgcttcttgc gccgttttgc agttgccaat catgttgatg
     2821 tctgctccgg cgataaaatt gtccggtttc gcggagataa acaccacgcc gcgcaactct
     2881 ttgttttcac ggagttgctt aataatggcg cgcacctgcg aggcaaattc cgccttcagg
     2941 gtattcattt tctcatccgg tacatcgatg gtgatgatag caatgttgtc cagacgaaca
     3001 ttaagagtaa acgctgatgc catttccatt attccgcctc cagaaccatt gccgcgccaa
     3061 gcccaccggc agcacaggcg gtaactaaac caaatccacc gccgcgacgg cgaagttcat
     3121 gcaatgtctg ggtaatcatc cgcgcgccgg tcgccgcgaa gggatgcccg taagcaatcg
     3181 aaccgccaag cacgttaaat ttgctatcgt ccacttcgcc agtggcatgt gcacgcccca
     3241 gtacttcacg agcaaaacgt tcactaccca gcaactgaat attcgccagt gtctgagctg
     3301 caaaggcttc gtgcatatcg atcaatgtca gttcgctcat cgtcaaaccg gcacgttcca
     3361 gcgccagcgg tgttgaccag gctggaccga gcaacatgtc ctgccagaca tcaatcgcag
     3421 taaatgcgta gctgcgcaga taccccagcg gcaccagccc taattctttc gcccgggatt
     3481 cagtcatcag gatcaccgct gccgcgccat cggtcagcgg cgtactgttt gccgccgtta
     3541 ccgttccgtg tttgcgatca aacgccgggc gcagctttgc gtaatcggca agcgaggaat
     3601 taccgcgaat attgttgtct tcgacaagcg gttgtttata aggagggata aaggcagtca
     3661 tcacctcttc tttgagtttt ccgtctgacc atgcctgagc ggcacgctga tgcgaacggt
     3721 gcgctaatgc atcttgctgt tctcgggtga tgccgtaggt tttcgccatt tgttcggcgg
     3781 tgtcgcccat ccgcaagcca gtagaatatt ctgctaccgc aggcggtacg ggcattaagt
     3841 cgcacaaacg caggcgagag aagagtttca ggcgctggct catggttcga gctttgttga
     3901 catcaaccag cacgcgcgcc agttttttac tgacgccaat tggcaatacg gaagaggaat
     3961 ctgccccacc ggcaatcccc gctcggatag ttcccgccat caggctttct gcgacgtttg
     4021 caaccgcctg gaaactggta gcgcaagcgc ggctgacgct gtaagcatcg gtatgcacat
     4081 tcattccggt accgagaaca atttcacgcg caatgttggg ggcttcaggc atttgtacga
     4141 cctgaccaaa gaccagctgt tcaatcactt cagcggggat ctcgctgcgt gccagcagtt
     4201 cgcctaccac catcttccct aaatcaaccg cgggaatgcc atgaaaagcc gtcgcctggc
     4261 gggcaaaagg cgtacgtaaa ccgctaacaa tggcgatacg atcgccctgg cgggtaacca
     4321 gcggtaaaac ctgacccata acactcccct gtaaaaaaat aaataaagtg gtctgacctg
     4381 atcatagtct taactatttt tttacattta gccaagcgga gaaaaaggaa agtgggagct
     4441 atgacacagt gaagacagga gaaagaaaag aaaacgcccc tgccagcaac tgacaggggc
     4501 gaacgtaccc gagaggaatt aacgcagacc cagctggaag atcagcattt cagcttcgca
     4561 ggcaaaagtg aaatcgatat ccaggcgcac accgtcagac tcttcagtga aagtcgggga
     4621 gattttgcac ggttcggatt ccacgctacg ggctttttca gtcagcgccg ccagcgtttg
     4681 ttctgcttct gcgcggtttg caaacacgcg gctgtaagac gcggtgcagt cggagttgtc
     4741 cataatggtg ccaacatcca tacagcagca aaccggggtt tcatcagcac tacatttact
     4801 catcgttgat ttcctctgta tgtgcaccca aggtgccaga taaacgttgt ggatatttta
     4861 cgcttccgga aagtgctgct ccagttgtta attctgcaaa atcggataag tgaccgaaat
     4921 cacacttaaa aatgatctaa aacaaaattc acccgaatcc gcgagtgcgc aacctccaaa
     4981 ttttgccagc tggatcgcgt ttcttagatc atatttgaaa aaagatggaa acatacttgc
     5041 agcattccag ctggtccgac ctatactctc gccactggtc tgatttctaa gatgtacctc
     5101 agaccctaca cttcgcgctc ctgttacagc acgtaacata gtttgtataa aaataaatca
     5161 ttgaggttat ggtcatgagc cagaaaaccc tgtttacaaa gtctgctctc gcagtcgcag
     5221 tggcacttat ctccacccag gcctggtcgg caggctttca gttaaacgaa ttttcttcct
     5281 ctggcctggg ccgggcttat tcaggggaag gcgcaattgc cgatgatgca ggtaacgtta
     5341 gccgtaaccc cgcattgatt accatgtttg accgcccgac attttctgcg ggtgcggttt
     5401 atattgaccc ggatgtgaat atcagcggaa cgtctccgtc tggtcgtagc ctgaaagccg
     5461 ataacatcgc gcctacggca tgggttccga acatgcactt tgttgcaccg attaacgacc
     5521 aatttggttg gggcgcttct attacctcta actatggtct ggcaacagag tttaatgata
     5581 cttatgcagg cggctctgtc gggggtacaa ctgaccttga aaccatgaac ctgaacttaa
     5641 gcggtgcgta tcgcttaaat aatgcatgga gctttggtct tggtttcaac gccgtctacg
     5701 ctcgcgcgaa aattgaacgt ttcgcaggcg atctggggca gttggttgct ggtcagatta
     5761 tgcaatctcc tgccgggcaa actcctcaag ggcaagcatt ggcagctacc gccaacggta
     5821 tcgacagtaa taccaaaatc gctcatctga acggtaacca gtggggcttt ggctggaacg
     5881 ccggaatcct gtatgaactg gataaaaata accgctatgc actgacctac cgttctgaag
     5941 tgaaaattga cttcaaaggt aactacagca gcgatcttaa tcgtgcgttt aataactacg
     6001 gtttgccaat tcctaccgcg acaggtggcg caacgcaatc gggttatctg acgctgaacc
     6061 tgcctgaaat gtgggaagtg tcaggttata accgtgttga tccacagtgg gcgattcact
     6121 atagcctggc ttacaccagc tggagtcagt tccagcagct gaaagcgacc tcaaccagtg
     6181 gcgacacgct gttccagaaa catgaaggct ttaaagatgc ttaccgcatc gcgttgggta
     6241 ccacttatta ctacgatgat aactggacct tccgtaccgg tatcgccttt gatgacagcc
     6301 cagttccggc tcagaatcgt tctatctcca ttccggacca ggaccgtttc tggctgagtg
     6361 caggtacgac ttacgcattt aataaagatg cttcagtcga cgttggtgtt tcttatatgc
     6421 acggtcagag cgtgaaaatt aacgaaggcc cataccagtt cgagtctgaa ggtaaagcct
     6481 ggctgttcgg tactaacttt aactacgcgt tctgataacg cgttcgcctg gataaagtca
     6541 cctgcatagc aggtgacttt aactccccca cttcaccgaa agtagtgtcc tcattgctta
     6601 cctcctgagt tttgcaaaca gcctgttggc agcttgccct ttcaaatcaa taagcggtat
     6661 ccacagacaa acctgaaggg aaaggcatat tttcaggcgt tctgctcgtc cttctcaaag
     6721 agtttacttt tctgcatttc caggatac
//
LOCUS       DAZPJR010000202         6649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPJR010000202 DAZPJR010000000
VERSION     DAZPJR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.207"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..739)
                     /locus_tag="ACHVB7_003887"
     CDS             complement(<1..739)
                     /locus_tag="ACHVB7_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR1915754.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDI"
     gene            complement(880..2913)
                     /gene="metG"
                     /locus_tag="ACHVB7_003888"
     CDS             complement(880..2913)
                     /gene="metG"
                     /locus_tag="ACHVB7_003888"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR1915755.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3045..4154
                     /gene="apbC"
                     /locus_tag="ACHVB7_003889"
     CDS             3045..4154
                     /gene="apbC"
                     /locus_tag="ACHVB7_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR1915756.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4399..4680
                     /locus_tag="ACHVB7_003890"
     CDS             4399..4680
                     /locus_tag="ACHVB7_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR1915757.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4923..5465
                     /locus_tag="ACHVB7_003891"
     CDS             4923..5465
                     /locus_tag="ACHVB7_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1915758.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5545..6219
                     /locus_tag="ACHVB7_003892"
     CDS             5545..6219
                     /locus_tag="ACHVB7_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1915759.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6235..>6649
                     /locus_tag="ACHVB7_003893"
     CDS             6235..>6649
                     /locus_tag="ACHVB7_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1915760.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1756 a         1508 c         1594 g         1791 t
ORIGIN      
        1 caatatcaat ctgttgaagg tgtattatta catcagtagc atattccaga ccgccttcct
       61 ggacgatctg atccataaca tcctcacgca ctttatatct tgtgcatttc atttcaagtg
      121 ccgtgagtac agccattact ggtgatggta actgtgttgt tttcaggccg tcgatctgac
      181 tgatcgcctg ggcgacggtg ttttgccatt ctaaagaaca ggcgctgaag tcaaatgtgg
      241 ctgttttatt atcccgttgc gacacactac aggctaagcg acgtaattgg gtcaatttgt
      301 ctgcttgctg aacaggaact ccgggaaagc ggcgatgact taacgtggtg tgtacaactt
      361 cgggcggaaa aacgacagca atatctttcg ccagccacgg taaaacagca tctgtatttt
      421 tgtcaggcgc acgggtttct gctacgaaag agggttgttc ctgaataatg gtggataagt
      481 ccgctacctt gagagagccc gttaccgttt gggaaggttg caaagaatta tccttcgctt
      541 gctccacata ccccttcttc accttctccg caatcagctt aagctccgct tttgccgctg
      601 ccgcagcatc tgaaaaactt tttatctggc tttgcccttt ggtgccaact tttccccaac
      661 tgatatgcaa ctcgtttccc tgttgctcaa ctgcccagaa tttatgtgat ttttcgtcct
      721 gatagataaa gtgtctcatt ctgcatccct gtaattttat ccagttgaat gcagatgcta
      781 ccagtattta tgcgggttag agagagacaa aatcgcaaga aaacaacttt aggaatttat
      841 aaagaaaagg cgctgtcgat gcagcgcctt gaagggggat tatttcacct gatggcctgg
      901 tttagcacca gcatccgggc ttaacaggaa gatatctttc ccgccaggac cagcagccat
      961 taccatgcct tcggagatac caaagcgcat tttacgcggt gccaggttgg ccaccataat
     1021 ggtgtgacgc ccgatcagtg cctgcggatc cgggtaggca gaacgaatac cggagaagac
     1081 attgcgtttt tcaccgccga gatccagtgt caggcgcagc agtttgtcag aaccttcaac
     1141 aaactctgcg ttttcgatca gcgccacgcg caggtcaact tttgcgaagt cgtcaaaggt
     1201 gatggtttcc tgaatcggat catctgccag tgggccagta actggcgcgg cagctgcttt
     1261 cacttcttct ttagacgctt ccaccagtgc ttcaacctgc ttcatatcga tgcggttata
     1321 cagcgctttg aacggattca ctttgtggcc cagcagcggt tgctggatac catcccaggt
     1381 cagttctgta ttgaggaatg cttctgcgcg ctcggtcagt ttcggcagta ccggcttcag
     1441 gtaagtcatc agcacgcgga acaggtttat gcccattgag caaattgcct gcaggtcggc
     1501 atcgcggcct tcctgttttg ccaccaccca cggagcctgc tcgtcgacat agcggttagc
     1561 cagatcagcc agcgccatga tttcgcgcac tgctttacca aattcgcggc tttcccacgc
     1621 ttcaccaatc acttcagcgg catcagtgaa ggttttgtac aactgcgggt cagccagttc
     1681 gcttgccagc acgccgtcaa aacgcttgtt gataaagccc gcattacgag aggccaggtt
     1741 aaccactttg ttcacgatat cggcattcac acgctgaacg aaatcctcca ggttgagatc
     1801 gatatcatca atgcgcgaag agagtttcgc agtgtagtag taacgcaggc tgtcagcgtc
     1861 aaaatgattc agccaggtgc tggctttaat aaaggtgccg cgagacttgg acatctttgc
     1921 gccgttcacc gtcacatagc catgaacaaa caggttggtc ggcttgcgga agttgctgcc
     1981 ttccagcatg gcaggccaga acaggctgtg gaagtaaaca atatctttac cgatgaagtg
     2041 gtacagctcg gcggtggagt ctttcttcca gtattcatcg aagcttacgc tgtcgctgcg
     2101 cttgtcgcac agattcttga aagaacccat gtagccaatc ggtgcgtcca gccagacgta
     2161 gaaatatttg cccggcgcgt tcggaatttc aaaaccaaag tatggcgcgt cgcgggagat
     2221 atcccactgt tgcaggccgg attcaaacca ctcctgcatt ttatttgcca cctgctcctg
     2281 caacgcaccg ctgcgggtcc atgcctgcaa catttcgctg aaagagggca gatcaaagaa
     2341 gaagtgttca gaatcacgca ttaccggcgt agcgccagaa accaccgatt tcggctcgat
     2401 caattcagtc gggctgtagg tcgcgccgca gacttcgcag ttatcgccgt attgatccgg
     2461 ggatttacat ttcgggcagg tgcctttcac aaaacggtcc ggcaggaaca tgcctttttc
     2521 cggatcgtac agctgagaga tggtgcggtt tttaataaaa ccgttctctt tcaggcgaga
     2581 gtagataagt tctgacaact ggcggttctc ttcgctgtgc gtcgagtgat agttgtcata
     2641 gctgatgtta aagcctgcga aatcagtctg atgctcctga ctcatttcgc caatcatctg
     2701 ctccggggtg ataccaagct gctgagcttt cagcatgatc ggtgtaccgt gggcatcgtc
     2761 ggcgcagatg aagttgacct cgtggccgcg cattcgctgg taacggaccc agacatcagc
     2821 ctggatgtgc tccagcatat ggccgaggtg gattgagccg ttagcgtacg gcagtgcgca
     2881 cgtcaccaga attttcttcg cgacttgagt catagtaggc attacttctt aatagtgaaa
     2941 aggggttaga tagtaccaaa tgggaaaatg ttaagtaagt acttctctat attcttcagg
     3001 taaactatca gctgatggtt tatctataca aaaaaggagt cgggatgaac gaacaatccc
     3061 aggccaaatc accagaagcc ttgcgcgcaa tggtcgccgg gacactggcc aattttcagc
     3121 acccaaccct gaagcataac ctcaccacgc tgaacgcgtt gcaccatgtc gcctggatgg
     3181 acgacacgtt gcatgtggaa ctggttatgc cattcgtctg gcatagcgcg tttgaagagt
     3241 taaaagagca atgtagcgcc gaactgctgc gtatcaccgg ggcgaaagct atcgactgga
     3301 aactgtcgca caatatcgcc accttaaaac gcgtgaagaa tcagccgggt attaatggtg
     3361 tgaagaacat cattgcggtc agttccggta aaggcggcgt gggtaaatcc tccacggcgg
     3421 taaacctggc gctggcactg gcggcggaag gggcgaaagt cggcattctg gacgccgata
     3481 tctacggccc atcaattccc accatgctgg gcgcagaaaa tcagcgtcca acttcgccgg
     3541 acggtaccca tatggcaccg attatgtctc atggcctggc aaccaattca attggttatc
     3601 tggtcactga cgacaatgcg atggtgtggc gtggaccgat ggcgagcaag gcgttaatgc
     3661 agatgttgca ggaaaccttg tggccggatc tcgactatct ggtgcttgat atgccgccag
     3721 gcaccggtga cattcaattg acgctggcgc aaaacattcc agtaactggt gcagtcgtgg
     3781 tcaccacccc gcaggacatc gctttgatcg atgcgaaaaa aggcattgtg atgttcgaga
     3841 aagtcgaggt tccggtgctt ggtattgtcg agaacatgag tgtgcatatt tgcagtaact
     3901 gtggtcacca tgaaccgatt ttcggtaccg gtggtgcaga gaaactggct gaaaaatacc
     3961 acacacagct gctggggcag atgccgttac acatctcctt acgtgaggat ctcgataaag
     4021 gcacgccaac ggttatcagc cgtccggaga gcgaatttac cgctatctac cgccagctgg
     4081 ctgaccgcgt tgcagctcag ctctactggc agggtgaagt cattccaggc gagatttcct
     4141 tccgcgcggt ctaacgccta ttaaccattc tggttattct tcatacaccg cagagatgtt
     4201 acattgatgc ggtgtattag tttttgccgc attaatgaac acttatttaa tactgcgaat
     4261 attctgcgga ccttcgtccg aaactctaca aagtcgattg atcatacagg caatgcttca
     4321 ttatcatcct tcgtggctgt gtagtggatg tacagctata gtttgttatg cactcattct
     4381 atccaggcat aaccttcaat gaataagtat tggctgtcag gaattatttt tcttgcctat
     4441 ggattggctt ctcccgcatt ctcttcagag actgccacat tagcgattaa tggcaggatc
     4501 agtcccccaa catgcagtat ggctatggtt aatggtcaac ctcaaccgca ttgtggtcag
     4561 ttaacgtata atgttgacac ccgtcatctg ttttcttcgc ccgtcaaagg tgtaacgaca
     4621 gaagtggttg ttgcaggtag cgatagcaaa cgtcgaatcg tgttaaatcg ctacgattaa
     4681 tttggcaacc tgatttttat tgatatcaac aataccattt acataaatct acattaattg
     4741 atagatgcga taaatagtta atactcttaa atatttgttg tatttatatt gcaatggtta
     4801 atttgtttca aataaataaa acaatgtgtt tacattgcgg taactttttt gatgtgagaa
     4861 atttgtttcc aagagataca gactcttaac aaccaaaaaa aaattttatt aaggaacata
     4921 ttatgaaacg ttcaattatt gctgccgctg tcttttcttc tttttttatg agcgctggag
     4981 tatttgctgc agacgttgat accggaacat taactattaa ggggaatatt gcagaatctc
     5041 cgtgtaaatt cgaagcgggt ggtgattcag taagtattaa tatgccgact gtaccaacca
     5101 ctgtctttga aggtaaagct aaatattcta cctatgatga tgcagtcggt gtaaccagca
     5161 gcatgttaaa aattagctgc ccgaaagaag ttgctggtgt aaaactctcg ttgattacca
     5221 acgataaaat aaccggtaac gataaggcga tagccagtag caacgatacc gtgggttact
     5281 atctctattt aggtgataac agcgatgtcc tggatgtttc tgcacctttt aacattgaga
     5341 gttataaaac agcggaaggt caatatgcta ttccgtttaa agcaaaatac ctgaaactga
     5401 cagataactc agtgcaatca ggtgatgtgt tatcttctct ggttatgcgt gtggcgcagg
     5461 attaataaat aaaattgtaa gtttctccag gtaataaaca tggccgctat cccatggcgg
     5521 ccttttaatt taagaggcat taaaatgaaa ggattattat ctttactcat tttttctatg
     5581 gtccttcctg cacatgccgg aattgttatc tacgggacgc gcattattta cccggcagaa
     5641 aataaagaag tgatggtgca gttgatgaac cagggaaacc gttcttcgct gctgcaggcg
     5701 tggattgatg atggcgatac gtcattacca ccagaaaaaa ttcaggttcc tttcatgtta
     5761 acgccaccag tggcaaaaat aggggcaaat tccgggcagc aagtaaaaat caaaattatg
     5821 ccgaataaac tgcccactaa taaagaaagc attttttatc tgaatgttct ggatattcca
     5881 ccaaatagtc cagagcaaga aggtaagaat gcactgaagt ttgcgatgca aaacagaatt
     5941 aagttgtttt accgaccagc gggtattgct ccggtaaata aagcgacatt taaaaaattg
     6001 ctggtaaatc gcagtggcaa tggtttggtg ataaaaaatg actcagctaa ttgggtgacg
     6061 atttcggatg tcaaagctaa taatgtcaaa gtcaattatg aaactattat gattgccccc
     6121 ttagaaagtc agagtgttaa tgtcaaaagt aataatgcaa ataactggca tctgaccatt
     6181 atcgatgacc atggcaacta tattagtgac aaaatttaat tgcaggagct gcctatgttg
     6241 agaatgaccc cacttgcatc agcaatcgta gcgttattgc tcggcattga agcttatgca
     6301 gctgaagaaa cctttgatac ccattttatg ataggtggaa tgaaagacca gcaggttgca
     6361 aatattcgtc ttgatgataa tcaaccctta ccggggcagt atgacatcga tatttatgtc
     6421 aataagcaat ggcgcgggaa atatgagatt attgttaaag acaacccgca agaaacatgt
     6481 ttatcaagag aagttatcaa gcggttaggc attaatagcg ataacttcgc cagcggtaag
     6541 caatgtttaa catttgagca acttgttcag ggtgggagct atacctggga tatcggggtt
     6601 tttcgtctcg atttcagtgt cccgcaggcc tgggtggaag aactggaaa
//
LOCUS       DAZPJR010000203         6610 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPJR010000203 DAZPJR010000000
VERSION     DAZPJR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.208"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..849
                     /locus_tag="ACHVB7_003894"
     CDS             <1..849
                     /locus_tag="ACHVB7_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR1915761.1"
                     /translation="ITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYDLHSGKIISS
                     SELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEMLTSEQQPRVN
                     QVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNANIGREQMLA
                     LYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQRDRYMARQLL
                     SAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVDHADYVWFVA
                     PDTTKR"
     gene            857..1633
                     /locus_tag="ACHVB7_003895"
     CDS             857..1633
                     /locus_tag="ACHVB7_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR1915762.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            1802..4063
                     /locus_tag="ACHVB7_003896"
     CDS             1802..4063
                     /locus_tag="ACHVB7_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1915763.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYHSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4132..5307
                     /gene="senB"
                     /locus_tag="ACHVB7_003897"
     CDS             4132..5307
                     /gene="senB"
                     /locus_tag="ACHVB7_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR1915764.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(5833..6294)
                     /locus_tag="ACHVB7_003898"
                     /pseudo
     CDS             complement(5833..6294)
                     /locus_tag="ACHVB7_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6332..>6610)
                     /locus_tag="ACHVB7_003899"
     CDS             complement(6332..>6610)
                     /locus_tag="ACHVB7_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR1915765.1"
                     /translation="LTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGA
                     LLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT         1856 a         1548 c         1539 g         1667 t
ORIGIN      
        1 ataacgttag ctagtgttag tctgggagcc tgcagacaga atgtcaccat aaagcaggat
       61 gcgccagggc agaaagcttt cctgactgac gggcagattt acgatttgca cagtggcaaa
      121 attatttcta gcagcgagct tttggcggat cttgctaccg ctcgacatct tattattggt
      181 gaaaaacacg ataatgccga gcatcatcag attgagcttt ggcttattca aaatttgctc
      241 atccagcgac cacaggggag tgtgttgctt gagatgttga cgtcagagca acagccccga
      301 gttaaccaag ttaaatgttg gctgaaagat aatcctgtgg ttcgagatag ccgtgttcag
      361 gaactactta actggcaaaa agggtggtcc tgggagatgt atggcgacat cgtcatgcag
      421 ctattacgtg ggccttaccc gctgctcaat gccaacattg gccgggagca gatgttggca
      481 ctgtacaaaa aaaacgaatt tcctaaagga aaaaaatcca ctgcacctgt ggtacaagaa
      541 gctctgcgtg aaaccatcat atctatgcat gaaggcaacc ttgaaagcca gcaactgacg
      601 tcaatgctct ctattcagca gcagcgggat cgctacatgg cccgccagct tctgagtgcc
      661 ccggtaccgt cgttgttgat tgcaggtggt tatcacgcgt caaaaagcat gggcgtaccg
      721 ttacacatgg aagatcttgc caccggcacc catccagtgg tgttgatgct tgcagaaaaa
      781 ggaatgaaca tcacagtaga tcatgctgac tacgtgtggt ttgtagcgcc agatacaaca
      841 aaaaggtaaa ccatggatga tgaatattct ccacttctca cagtcggtaa aatggagcag
      901 ctggtttatc tgttctctac tgcttcatgg actgattttc cttgcgttta tctggcgttt
      961 ttccgaagtt cagcccgcta tgtctcctgc accggcgatc atgctgcaat gggcagaaat
     1021 tatagaggct ccttcttctc cactttcgct tccggtggga atcgctcaac aggagtccgc
     1081 agtgacagaa gaaaagcaac aaactgagga cagacaacaa cgtcctgtca ctgaagacag
     1141 tgatgcaacc attgaaataa ctaggaagaa aaagtcatcc gatggagaaa agaaaaagac
     1201 acgtccacct cgtaagataa aggctcaaac cagcgacagc aaccccacag ccgtttccag
     1261 taatgctgca cctcaggctc tggtcgaatc gtcccgtatt gctgcgcctt ttaacagtga
     1321 ctcaacaaag cgagataatt ctgaggcatc ttgggaaagc agagtaaaag gccacctaaa
     1381 ccgttataaa cgttaccctg gcgatgccag aaaacgtgca cgaaccggca cagccgtcgt
     1441 gacttttacc gtaaacacgg aaggcactat tgtcagtagt ttcttggaaa tctcttcagg
     1501 aaccctttct cttgaccgcg aagctattgc tgttttagaa cgcgctcaac cacttcccaa
     1561 accaccgcct gaaatattgg agggaggatt atttaaagta aaaatgccga ttacctttaa
     1621 gttgaaggag taaagggata attggccgtt caagttcatt taactgaaat ttaattttca
     1681 tttttatgct gaacatttta tcaaactttt ccactgtcga aaaaatcaaa aagacagtag
     1741 aacataatta ataaacaaat agttatactt atctttatta atattatacc caaggaaatt
     1801 tatgaacgtt ataaaactgg ctatcggctc aggcatatta ttgctcagct gcggtgctta
     1861 ctcacaatcc atcagtgaaa aaactaattc cgacaaaaaa ggagcggcag aattcagtcc
     1921 gctcagcgtt tctgtcggga agacgaccag tgagcaggaa gctctcgaga aaacaggcgc
     1981 gaccagttcc cggacaacgg acaaaaacct gcaatcactt gacgcaacag tgcgtagtat
     2041 gcctggtact tatactcaaa tagatcctgg tcagggagca atcagtgtga atattcgagg
     2101 catgagcgga tttggtcgtg taaacactat ggtcgatggt attacccaga gtttttacgg
     2161 aacctctacc tccggaacaa cgacgcatgg ttcaactaac aatatggctg gcgtacttat
     2221 agatcctaac ttactggtag cagttgatgt tacacgcggt gacagcagtg gctctgaagg
     2281 gatcaacgcc cttgccggta gtgcaaatat gcgtactatt ggcgttgacg atgtaatatt
     2341 taacggtaat acatatggcc ttcgttcacg tttctctgtc ggtagtaatg ggctgggacg
     2401 cagcggaatg atcgcccttg gtggaaaaag cgacgctttt acggatacgg gaagcattgg
     2461 cgttatggct gctgtgagcg gcagttctgt gtactctaat ttctcaaatg gttctggaat
     2521 taacagcaaa gagtttggtt atgataaata tatgaagcag aaccccaaat cccaactgta
     2581 taaaatggat atcagaccag acgaatttaa cagcttcgaa ctttccgctc gaacctatga
     2641 aaataaattt acacgtcgtg atataaccag tgacgactat tacattaaat atcattacac
     2701 ccctttttct gaattaattg actttaacgt aacggccagt accagtcgcg gtaatcaaaa
     2761 gtatcgtgat ggctcgctgt atactttcta caaaacctca gcgcaaaatc gttctgacgc
     2821 gctggatatc aacaatacca gccggttcac tgtcgcggac aatgacctgg agtttatgct
     2881 gggcagcaaa ctgatgcgta cccgctatga ccggaccatt cactcagcgg cgggcgaccc
     2941 gaaagcgaat caggaatcga tcgagaacaa tccgttcgca ccctccggcc agcaggatat
     3001 ttcagcgctg tataccgggc tgaaggttac gcgcggcatc tgggaggcag atttcaatct
     3061 caactacaca cgtaacagga tcacagggta caagcccgcc tgcgattcac gcgttatctg
     3121 cgtgccacag ggtagctacg atattgacga taaagagggt ggcttcaacc cttcagttca
     3181 gctttctgct caggtaacac catggcttca gccgttcatt ggctacagca aatccatgcg
     3241 cgccccgaac atccaggaga tgttcttctc taattcagga ggcgcatcca tgaacccatt
     3301 cctgaagcct gaacgtgcag aaacctggca ggcgggtttt aacattgata ccagagattt
     3361 actggtcgaa caggatgccc tgcgctttaa ggctctggcg taccacagca ggatccagaa
     3421 ctacatctac agcgagtctt atctggtttg ttctggaggt cgtaaatgca gtatgcctga
     3481 ggtgattggc aatgactggg agggcattag cgatgaatac agcgacaata tgtacatcta
     3541 cgttaactcg gcaagcgacg ttatcgcaaa gggcttcgaa ctggagatgg attatgatgc
     3601 aggttttgct tttggccgac tctctttcag ccagcagcaa acagaccagc caacctccat
     3661 cgccagcacc tactttggcg caggggatat gaccgaactg cccagaaaat acatgacgct
     3721 ggatactggt gttcgcttct tcgataacgc gttgaccctg ggcactatca taaaatacac
     3781 aggcaaggct cgtcgcctgt cgcctgattt tgagcaggac gaacataccg gcgcaataat
     3841 caaacaggat ttgccgcaga tcccaacgat tatcgatctc tatggtactt acgagtacaa
     3901 ccgcaaccta acactgaaac tttcggtaca aaacctgatg aacagagatt attcggaggc
     3961 gctgaataag ctcaacatga tgccaggtct tggtgacgag acccacccag ccaattccgc
     4021 gcgtggcaga acatggatat ttggcgggga cattcgtttc tgatgacaat ttccgctagg
     4081 ttcagacaat acgttttctc tcttatgtca attttattgc aggaacgaaa aatgaatatt
     4141 ttcactttat ccaaagcacc gctatacctg ttaatttcac tatttttacc cacgatggcc
     4201 atggctatcg atccacctga acgcgaactt tcgcgatttg ccctgaaaac gaattacctt
     4261 cagtcccctg atgaaggcgt ctatgaactg gcgtttgata atgccagtaa aaaggtgttt
     4321 gcagcagtca ccgatcgtgt aaatcgtgaa gccaataaag gctatctgta ttcgtttaat
     4381 tcagattcgc tgaaagtcga aaataaatac acgatgccat accgggcatt ttcgctggcg
     4441 ataaatcagg ataaacatca gctctatatc ggacacaccc agtcagcgtc cctgcgtatc
     4501 agtatgtttg acaccccaac cggcaaactg gtaagaacca gcgacaggtt aagttttaaa
     4561 gcggcaaacg ctgcagattc gcgttttgag cattttcgcc atatggttta cagccaggat
     4621 tccgataccc tgtttgtgag ttatagcaat atgctgaaaa cggccgaggg catgaagcct
     4681 ctgcataagc tgttaatgct cgacgggacg acgcttgcct taaaaggcga ggttaaggat
     4741 gcttacaaag gtacagcgta tggtctgacg atggatgaaa aaacacagaa aatctacgtt
     4801 ggcggaagag attacatcaa cgaaattgat gcgaaaaatc agacgctgct gcgtaccatc
     4861 ccgttgaaag atccgagacc acaaatcaca agtgtgcaga atctggcggt ggactccgct
     4921 tctgaccgtg cctttgtggt ggtattcgac catgacgatc gttccggtac aaaagatgga
     4981 ctctatattt ttgacttacg cgacggtaaa cagcttggct atgtgcacac aggagccgga
     5041 gctaacgcgg tgaaatacaa tccgaaatat aacgaactgt atgtcaccaa cttcactagc
     5101 ggcaccatca gcgtagtgga tgccaccaaa tacagcatca cccgtgaatt taacatgccg
     5161 gtctacccaa accagatggt gttgtcggac gatatggata ccctttacat tggcatcaaa
     5221 gaaggcttta accgcgattg ggatcctgat gtgtttgtgg aaggagctaa agaacgtatt
     5281 ctgagcattg atttgaaaaa gtcgtgatcg gggggaaaaa acatgggata aaaactccgt
     5341 tattccacta aactggccgg actaggaaaa gcaaacatct ctgtcctgcc ggtacgcctg
     5401 ttacgccgct ccatgaggct gaggaatctc cagaaaacga gacaggcaca gcattgtacg
     5461 atcaactgat ttgtgccaat aattaaatga aatcacagct atgcctgttc atggggttct
     5521 aggccggaac cccagatttt tccgattgtt tgttttctaa ctgattattc agtagtttta
     5581 gagggctgac gctcagtgga acgaaaactc acgttaagag gttttgacta tgataaaagt
     5641 caaagtaggg gtgagtttgg atatggaaat tattgtacta taaggagagc atgaaaaccc
     5701 ctggggcagt cactgaatga gggaccagca gcggtaaaat aaccagcata atttctgcat
     5761 ggtcgatttt tataccgtga caggctgttt tttatacttg gggtcagttt ggatatcgaa
     5821 aattattgta cgttaaggtt gttttttgac cttgtcgatc ttaactgatc tcaaacgacg
     5881 aaacttacct gaagtcggta tccggctgtt tttccagata taatcgcctg tcagattgat
     5941 atgttcccaa cctaatggtg ataaatgcga taatagttga gcatttattt tcatgccctt
     6001 acgcttcaga gcatcaaccg ctctttccat gtaaactgtg ttccataagg aaatagccgc
     6061 cgtcaataaa gtcaatccac tggcgcgata actttgattt tccggtttcc gatctctaat
     6121 ctcccccaat cgatgcatga atacagcacg ggccagagca ttacgggctt ctcctttgtt
     6181 cagcccggcc tggacacgtc gccgcaatgc cggatcccga aaccagtcca gcataaacag
     6241 tgttcgttct atacggccaa tctctctcag agctttagcc aacccatttt gtttgtaagc
     6301 gtgcagcaag aaccgtattg acgggaatgt gttattcagt cggcagtgct acgcgccagg
     6361 ggagcagttc gccgacccgg tttatcggcc agtcggctat gacgtcaagg acatagcgga
     6421 ggtagctttc tggctccact ccgttcagtt tgcacgtccc gatcaggctg tacagcagcg
     6481 ctccccgctc tcctccatga tccgaaccga agaacaggta gtttttgcgg cccagactga
     6541 ccatccgcaa cgcattttca gcgatgttat tgtccgcctc agcccagcca tcatctgcat
     6601 agtacgtcag
//
LOCUS       DAZPJR010000204         6537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPJR010000204 DAZPJR010000000
VERSION     DAZPJR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.209"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHVB7_003900"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHVB7_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR1915766.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHVB7_003901"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHVB7_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR1915767.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHVB7_003902"
     CDS             2408..3157
                     /locus_tag="ACHVB7_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR1915768.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="ACHVB7_003903"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="ACHVB7_003903"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHVB7_003904"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHVB7_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR1915769.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="ACHVB7_003905"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="ACHVB7_003905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR1915770.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="ACHVB7_003906"
     CDS             6018..6491
                     /locus_tag="ACHVB7_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR1915771.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1650 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgtta
//
LOCUS       DAZPJR010000205         6532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPJR010000205 DAZPJR010000000
VERSION     DAZPJR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.210"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHVB7_003907"
     CDS             complement(<1..906)
                     /locus_tag="ACHVB7_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR1915772.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            complement(1331..1474)
                     /gene="ypaB"
                     /locus_tag="ACHVB7_003908"
     CDS             complement(1331..1474)
                     /gene="ypaB"
                     /locus_tag="ACHVB7_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR1915773.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="ACHVB7_003909"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="ACHVB7_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1915774.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="ACHVB7_003910"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="ACHVB7_003910"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1915775.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="ACHVB7_003911"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="ACHVB7_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR1915776.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5559..6208)
                     /gene="inaA"
                     /locus_tag="ACHVB7_003912"
                     /pseudo
     CDS             complement(5559..6208)
                     /gene="inaA"
                     /locus_tag="ACHVB7_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(6288..6392)
                     /locus_tag="ACHVB7_003913"
                     /pseudo
     CDS             complement(6288..6392)
                     /locus_tag="ACHVB7_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6387..6532
                     /locus_tag="ACHVB7_003914"
                     /pseudo
     CDS             6387..6532
                     /locus_tag="ACHVB7_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1686 a         1686 c         1526 g         1634 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga ca
//
LOCUS       DAZPJR010000206         6515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPJR010000206 DAZPJR010000000
VERSION     DAZPJR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.211"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHVB7_003915"
     CDS             38..250
                     /locus_tag="ACHVB7_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915777.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHVB7_003916"
     CDS             283..501
                     /locus_tag="ACHVB7_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1915778.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHVB7_003917"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHVB7_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHVB7_003918"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHVB7_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR1915779.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHVB7_003919"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHVB7_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHVB7_003920"
     CDS             1917..2660
                     /locus_tag="ACHVB7_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR1915780.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHVB7_003921"
     CDS             2690..3229
                     /locus_tag="ACHVB7_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR1915781.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHVB7_003922"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHVB7_003922"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR1915782.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHVB7_003923"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHVB7_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR1915783.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHVB7_003924"
     CDS             4582..6102
                     /locus_tag="ACHVB7_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1915784.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6515)
                     /locus_tag="ACHVB7_003925"
     CDS             complement(6135..>6515)
                     /locus_tag="ACHVB7_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915785.1"
                     /translation="IANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKK
                     NTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQ
                     ASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1714 a         1489 c         1550 g         1762 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaat
//
LOCUS       DAZPJR010000207         6480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPJR010000207 DAZPJR010000000
VERSION     DAZPJR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.212"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..438
                     /gene="ydjO"
                     /locus_tag="ACHVB7_003926"
                     /pseudo
     CDS             1..438
                     /gene="ydjO"
                     /locus_tag="ACHVB7_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(442..1833)
                     /gene="tcyP"
                     /locus_tag="ACHVB7_003927"
     CDS             complement(442..1833)
                     /gene="tcyP"
                     /locus_tag="ACHVB7_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR1915786.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1966..2556)
                     /locus_tag="ACHVB7_003928"
     CDS             complement(1966..2556)
                     /locus_tag="ACHVB7_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1915787.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2719..3387)
                     /gene="hxpB"
                     /locus_tag="ACHVB7_003929"
     CDS             complement(2719..3387)
                     /gene="hxpB"
                     /locus_tag="ACHVB7_003929"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR1915788.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3534..4070
                     /locus_tag="ACHVB7_003930"
     CDS             3534..4070
                     /locus_tag="ACHVB7_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR1915789.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4111..4971)
                     /locus_tag="ACHVB7_003931"
     CDS             complement(4111..4971)
                     /locus_tag="ACHVB7_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR1915790.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5077..5367)
                     /gene="ghoS"
                     /locus_tag="ACHVB7_003932"
     CDS             complement(5077..5367)
                     /gene="ghoS"
                     /locus_tag="ACHVB7_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1915791.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5468..6397)
                     /gene="pfkB"
                     /locus_tag="ACHVB7_003933"
     CDS             complement(5468..6397)
                     /gene="pfkB"
                     /locus_tag="ACHVB7_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR1915792.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1693 a         1605 c         1576 g         1606 t
ORIGIN      
        1 tgacgaattt gatcaatata aaaaataggt tggttattca ttatttaagg actatatact
       61 atgaaaaaaa ttatatacat cctgccggga gcatttttgc tgcgattcaa aaaaaatgag
      121 agtctcctaa agtttccttc gataatatca atgcttaata tcaatcttct ttttaatcca
      181 ctaaatcttc cgggaatggg aagtggtgtg cttgaagata ttatgagtat tccagatagt
      241 tcacttagaa aacgtctcgg gtatgaggtt ttaagcttct cactacaggc acattcattg
      301 agccaggaat gtattgataa gttggacatt tttttcgctg acgatctgtt taaatatgag
      361 tcagtatgca tcgccgcgat ggaacattta aaaagtaaag caacggcccc tatacaaaac
      421 ggaccgttgc cagcataaga attaacggtg tgccagttcg gcgtcgtctt cgctatccag
      481 aatggcttta tcggtttgct tcagccactg gctggtcagc gtgccagctg tcatcgagcc
      541 actaacgttt agcgccgtac ggcccatgtc gataagcggt tcaacggaga ttaacagcgc
      601 caccagggtt actggcaggc ccatcgcagg cagcacaatc agtgcggcga aagttgcacc
      661 accaccgaca ccggcaacgc ctgcggaact aacggtaaca ataccgacca gcgtcgcaat
      721 ccacatcggg tccagcgggt taatgccaac cgtaggcgca accatcaccg ccagcattgc
      781 cggatacaaa ccggcgcagc cgttctgacc aatggttgca ccgaaagagg cggcgaaact
      841 ggcgatggat tcaggaacgc ccagacgacg cgtttgtgct tccacattca gtgggataga
      901 cgcagcactg gaacggctgg taaaggcaaa cgtcagcaca ggccatacct tacggaagta
      961 cttcagcgga ctcacgccat taatgcccag cagaatgcca tgcactgcaa acataatcag
     1021 cagaccgagg taggacgcga caacgaaact tcccagtttg atgatgtctt gcaagttaga
     1081 acctgcaacc actttggtca ttagagccag aacgccgtaa ggggtcaact gcatgacaag
     1141 gcgaaccagt ttcatcaccc agctttgtag ggtatcgata gcggctaaga cgcgttcacc
     1201 tttcggcgca tcatccttca gcagtttcaa tgcagctacg ccgaggaatg cggcaaaaat
     1261 taccacgctg ataattgacg tcggattggc tccggtaaga tcggcaaacg ggtttttcgg
     1321 gataaaggac aagaccagct gcggaacgct cagatcagag actttaccaa catagttact
     1381 ttcaatggcg ttcagacgtg cagtttctgc accaccctga accagacctt cagccgtcaa
     1441 accaaacagg ttggtgacca gcacaccgac cagcgccgca atcagcgtgg taaacaaaag
     1501 cgtaccgatg gtcagaaaac tgattttgcc taactgagat gcgttatgca gacgggcaac
     1561 cgcgctcaga atagaggcga agactaacgg cataacgatc atttgcagca gttgaacata
     1621 gccgttacca acgatgttaa accactgtac agaatcttta agtacctggc tgtcagaacc
     1681 ataaatggta tgcagggcaa ggccaaaaac cacacccatc accagaccca ccagcacttt
     1741 tttcgccaga ctccactgtt tatggcgggt ctgagccagc gcaaacagca gtacaacgaa
     1801 caccacgatg ttcgcaatta atggaaagtt catccccgtt ctcctgatct tattatttct
     1861 atcactgcgt agcagtcata gtgttggcgc aagattaaca gaactgtgag ctggctctta
     1921 tattcaaatg gaattgctta ttccagaaag gtgatttttg cctttttagt attcaacctg
     1981 atgcttaata agccgatgaa actggatctg caaggtattg atcatttgcg atgaccaacg
     2041 aacagcgctg ttctcgggta atgaatgggg catccatacc gaccagacaa ataatgccat
     2101 gcagataaaa cgttccagtt ggttgccctt ttggggaacc aggataggca agcggaaacg
     2161 ccagcggcat ggccagagca ggggaacacc ggcgggtgtc agcatatcgg caagtatgtg
     2221 gctcaaataa cccagcacca ttccttgtag cgcatcagcc ggaatgaacc agccttccgg
     2281 aaccttaagg taaaaggttg ccagcagcgc aaataccgcc agcagactgt gggtaaaacc
     2341 acgatgccca aaagcgcggg cgatcggttt tgatatccat tttaatcgct gcccaagaaa
     2401 cgactttggg tgatcgatgt ccggtaacaa acacgtcagg attgcggaag ggacaatatg
     2461 ccaccagtca ccctgtgcca gcacgggcgt cagctcggca tttttggcaa ataccgcaca
     2521 agcaatagaa aagagaaggt gaccttccgc cgtcatgata aaactcacta aactgtcgat
     2581 acgtacagta taggattttt atacagtgca cggaagtggt gacggtgtaa ctctttctct
     2641 taaattgacg attaatccgt cagttgaatg caaattcaga taaagaacca ggggcagcgt
     2701 gatcactgcc cctggtcatt aaccgagaag gtcttttgcg gtgagttctg tcagcgatga
     2761 gagtttgacg tttgctaata caaaacgtgg atcattttgc gcttctggcg caggaacgac
     2821 gatggaacgc atgcgggctg ctttagaagc gatcatgcca tttaccgagt cttccagcgc
     2881 cacgcaggtc agtgggtcaa cgcccagttt tgctgcgcag tcgagatata cttgcggatg
     2941 cggcttgctg taaggcagtt tttcagccga ggcgagggca tcgaaactgt cgcgtaagtc
     3001 aaacatggtc aacacttttt ccagcatatg tagtggtgac gcggaggcca gtcccaccaa
     3061 taaaccttgt tctttgcata acgcaacagc ttcgcgcacg cctggtaata atggacgtgt
     3121 ctcttcaacc agtgaaatgg cacgggcaat aacccgttct actacttcct gacggcttgg
     3181 cccattccac ggttgccggg cgtaccaaag atcgaccacc atatcgatgc gtaaacctaa
     3241 ggtgtccggc agctcgttac gacgggagat atccaccccc aggcttgcca tcacatccag
     3301 ttcggctcga tcccgtaaag gttctgagtc gataagtaat ccatccatat caaaaattgc
     3361 agcaagaatc tgacgcgggg ttgacattac aacctctcct ttagagcatt atgtgtaagg
     3421 tattaagccg tgcttatgat taagcgccta tcatatcgcc tttaaagaat gggcgaaaac
     3481 agatatctac aggtaaacta agtgaaacga cgcgtcttaa tcaaggagaa ctcatgacgt
     3541 atcaacaagc tggacgcatt gctgttttga aaaggatttt gggttgggtg atttttatac
     3601 ctgcattgat ctccacattg atttctttgc tgaaatttat gaatacccgg caggaaaacc
     3661 aggaaggcat taatgcggtc atgctcgact ttactcatgt catgatcgat atgatgcagg
     3721 cgaatacgcc ttttttgaat ctgttctggt ataactcccc gacccctaat tttaatggtg
     3781 gcgtgaacgt tatgttctgg gtgattttca tcctgatttt tgtcggactg gcgttgcagg
     3841 attccggtgc ccggatgagt cgccaggcgc gcttcttgcg ggaaggtgtt gaagatcaac
     3901 tcattctgga aaaagccaaa ggggaagagg ggctaacgcg tgaacaaatt gagtcccgta
     3961 ttgttgttcc gcaccatact attttcctgc agtttttctc actatatatc ctgccggtta
     4021 tttgtattgc tgcaggttat gtgttctttt ctctgcttgg gtttatttaa tcaaaaggcc
     4081 gcacggagcg gccattatcc tcaacccata tcatgctgct aataatctat ccaatgactg
     4141 ctgagcaata accaaatgct gaccgccaaa taatcttgca cgatttaaca gcgtgtagag
     4201 ttggtaaacc ggttgacgtt caaggaaatc ggcaggtagc ggggatactg actgatagcc
     4261 gtcatagatt tgtggcggct gttcagtatg cagcggtaac atcgccaggt cgcactctcg
     4321 gtcaccccag tagcaggcag ggtcgaaaat gtacgggcca tccggaccca gcgcacagtt
     4381 gccggaccat aaatcgccgt gcaacagaga aggctgcagt tgatgcgagg ccagacgctg
     4441 ctgaatatgc tcgacgatgg cgtcgatatt gccgaaagcg atccctttct ctgctgccag
     4501 ttccaactgc cagccaatcc gttgttcagc aaaaaacgtt gaccagcgac gttgccaggt
     4561 gttgggctgt ggagttgtgg agagcgagtt atcaaaatcg aggccaaatt gtggttggtc
     4621 actccattga tgtaaacgcg caatttgctg accaagaata aatgcgctat gcgcatccag
     4681 cggacgaggt gggagataat ccatcaccag aaaactgtag tcacggtcag cgcctactgc
     4741 ccaaacctta ggcacggtga cggttttact acgcgacagt aactccagtt ggtcggcttc
     4801 ggcggtaaaa ccgggaagca gttccctttc atcacatttg acgaaaaagt catgtcctgc
     4861 atagcgcaaa tgccatgcgg catggacttc tccgccaggc agttcattac gcagttcgat
     4921 ttcgccttca cctaactgct cgctcaaaag acgactgatt gcctgccaca tgatgtctct
     4981 cccatgttgt ctgccagatc ataaggttag cgcaagaatg cggtgtaaaa acacgagcta
     5041 acgcacacca cagactgatt attgcccaaa aaccatttat tttctgttgc tattccattc
     5101 ctcccaagtc gtgatggtga tttcaggatc tttgccggtt gcactttggg taagccccga
     5161 aaccagttct ctaatttctt cttcactttg ggtactgata agtccaaaag tgttagtccc
     5221 cagctcatgg atatttccct catcatccgt catggtgagc aaaaaaccgt cgcgagtcag
     5281 gtaattattc aactcgttaa tttcagtcaa tgtatcctca tgcaacatga cggttatgac
     5341 ataacggaca agatcgccac tggccatagt tcaccccttt gttatcacat gacttttgag
     5401 caaagtcgga gaaacgcgtt gccgacaggt tggtggtgat tcccccaatg ctgggggaat
     5461 gtttttgtta gcgggaaagg taagcgtaaa ttttttgcgt atcgtcatgg gagcacagac
     5521 gtgttccctg attgagtgtg gctgcactcc ccgcagctac gccaaaacgg accatctctt
     5581 caagagaggc attttctgcc agtttcagtg tcatcgcgcc gaccatgctg tcaccagcgc
     5641 caacggtact ctggcttttc accggtggtg gcaccacctg aatgcagttt tcactatcaa
     5701 cacccagcgc tccttgtgga ccaagggaaa cgacaacccg tttggccttg ccgctattaa
     5761 cgatttcctg cgcggctttg cggacatcgt ccggctgggt gagttcgcga ttcaccagcg
     5821 cactgagttc tttttggtta ggcttaacca actcgatgtt accaattgcc agtgctgcac
     5881 ttaacgcttc gccagaactg tcgacgatgc agcggatccc ttgtttttgc gaagcggaaa
     5941 tcagttgggt taatttttcc agcttcacac ctggcggcag gcttccgctt atgaccagga
     6001 tggccccgga ttcaatttcc agaacttgct cttcaagctg gcgaaactca tcttcattta
     6061 atgccgcgcc tggcataaca aaacgatact gctcaccgct tgcttccaca tgtacatgta
     6121 aattctgccg ggtccagtct ttggcttcta cagtagcgac ggggacattt tcatccgcca
     6181 acagtgaaac caggtgttcg ccggtcgcgc cacccgccgg gaagatcgct gtggcactgc
     6241 ctccaagatg ggcaatggcg cgggcgacgt tgatgccgcc gcccccgggt tcgaacaccg
     6301 gtgcggtaca gcgcagtttt ccttcgggat aaatttgcgg ggtaattgtt gcgctatcga
     6361 gagagggcgc aagtgtcaac gtatagatac gtaccatcat ttcctcctat aggctgattt
     6421 cagtctggca ccgaatcagc ggaaagtgaa gaaattaaca atatgattta ttggagcttt
//
LOCUS       DAZPJR010000208         6389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPJR010000208 DAZPJR010000000
VERSION     DAZPJR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.213"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHVB7_003934"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHVB7_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1915793.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHVB7_003935"
     CDS             2219..2767
                     /locus_tag="ACHVB7_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR1915794.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHVB7_003936"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHVB7_003936"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR1915795.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHVB7_003937"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHVB7_003937"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR1915796.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHVB7_003938"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHVB7_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR1915797.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1611 a         1565 c         1615 g         1598 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcagtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatatt
//
LOCUS       DAZPJR010000209         6325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPJR010000209 DAZPJR010000000
VERSION     DAZPJR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.214"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..355
                     /gene="mcbR"
                     /locus_tag="ACHVB7_003939"
     CDS             <1..355
                     /gene="mcbR"
                     /locus_tag="ACHVB7_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1915798.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(391..2493)
                     /gene="pqqU"
                     /locus_tag="ACHVB7_003940"
     CDS             complement(391..2493)
                     /gene="pqqU"
                     /locus_tag="ACHVB7_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR1915799.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2735..3796
                     /locus_tag="ACHVB7_003941"
     CDS             2735..3796
                     /locus_tag="ACHVB7_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR1915800.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="ACHVB7_003942"
     CDS             complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="ACHVB7_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR1915801.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5677..6294
                     /locus_tag="ACHVB7_003943"
     CDS             5677..6294
                     /locus_tag="ACHVB7_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR1915802.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1704 a         1594 c         1513 g         1514 t
ORIGIN      
        1 caccccgcaa gacctgacgg aactccagga attgctcgag aaattacagc aggcgcaaga
       61 aaagggcgac atggaacaaa tcattaatgt aaacaggcta tttcgcttag cgatttatca
      121 tcgctcaaat atgcccatcc tgtgtgagat gattgagcaa ctgtgggtca ggatggggcc
      181 tggtttacat tatctctatg aagcgattaa tccagcggaa ttacgggagc atatagaaaa
      241 ctatcatctg ttactcgctg cgttaaaagc aaaagacaaa gagggatgca gacattgtct
      301 tgctgaaatt atgcaacaaa atattgctat tttataccag caatacaatc gttaaagtaa
      361 atgcccggca aataccgggc atcaaggtaa ttactcaaat ctccacgcaa tattcatgcc
      421 gacaccataa tttcgtccgg gcgaaggttc gtaatatcgc ccgtttgact cattgacaat
      481 gacagaacca acgtattctt tatcgaataa attatcgaca cgaccaaata aatccacagt
      541 taaattgtgg taattgtatt tatacccggt gaataagccg acgagagtat aagacggcgc
      601 ttttgccgta ttttcatcat ctgccataat atcgcccata taacgcgctt ccgtgcctgc
      661 ataccaacca tcttccggta cataacctat cgacgcaaag cccatattac gggcgatccc
      721 tggcatccga ttaccgttac aatcctgttc attgcaaaca ttgctgcgat aggtcgcatc
      781 aagccaggtc caggacgcgt ttacgcgaaa atctcctgcg aaacgttgat cccatgccag
      841 ttcagcgcct tgacgacggg tctttccggc atttttgtaa gtcgtacgcc caccgctact
      901 gctatcgaca acaatttcat catcagtgtc ggtctgaaac aatgcgagac taagcagccc
      961 atcaccaata cgcgttttac tgccgatctc aattgtatcg ttggtggatg gttttaaacc
     1021 aaagttcata ccgctttgcc catcagcacg ataagacagc tcattaatcg tcggcgtttc
     1081 aaaacctcgc ccggctgcca gatagatatt ccaggcatcg gtcattgcat attttaacga
     1141 accggcaggt agccatttat gataactggc atcaccgctg tcatcgccgt tacccggagt
     1201 aacgtaatgg tcgttggaat caaaccacac ggagctgtag cgcacgccag catccagcga
     1261 cagtttttcg ctcagctgcc actgcgtctg taaataggga tcgatgttcc acatcagatt
     1321 gcgttcgtcg cgacgcaact cacctttttg cccgtactcc ggcatgccgc tattcaggcg
     1381 gaagttattg tagcccttgc ggttttcact catgttttcg tagttcaggc cggtagtgaa
     1441 cgtgaccgga acgcccagtt caccacggtg tgtccagcgg ctgtctatcc cctggtaatg
     1501 gcgttgcagg gtaatcacgc cgcccgcatg tgacgggtta agttgtggtg ccatgggtat
     1561 tgactggtac tgggtcgttt ctcgctctcc ggcatacatc atcacactca tatcatcttg
     1621 cgcgctcagg ctacgctcat agcgcaaccc agcctgagtt tgcttgatgg tttttcgcgt
     1681 gtcgtactgt tctgcacgag gcgcttgttg tggattagcc ttccattctg ctttggttag
     1741 cccacccggg tcatctgctt tgatatccac actattgaaa atcagactta atttgctggc
     1801 ttcatcaatg cgtacgccca gtttggcatt ggctaaattt ttctgtgcgc cactatggtc
     1861 acgatagccg tgggtcgtaa aacgcgtggt tgagacggtg taatcgacat cgccaggctg
     1921 tgtgccgtct cccgttgcgc ccgttgcttt cagcccatag cgccagctgc caaaactgcc
     1981 gtagtaacta ctggcttcaa tggttggtgg ctgttgtccg gtctgggtgg tgacattcat
     2041 taccccacca gacgcgttgc catacagggc agagaagggg ccacgcagca cttccacatt
     2101 ttgcacactg cttaaatcga tgttggatgt ttgcccttgc ccgtcgggca tggtggcggg
     2161 aataccgtcc acatacaggc gaataccgcg aataccgtaa gtggagcggg agccaaatcc
     2221 gcgaatcgac agctgtaaat cttgcgcata gttctgccgg ttttgtacct gcaaaccagg
     2281 cacgtcggtc agtgattcgg acaagttaat gcgcggtgtt gccaggcgca tctcctcgcc
     2341 atccaccacg cttactgctg ctggggtatc cagttctgaa accacctgcg gtgcggcact
     2401 gacaatcatg gtctgttcat cagcggcaaa aacaacgggg gaaagggcaa gtagtgcggg
     2461 caaaacggtc tgtcggacgg aaaaaatctt catgaaaaaa gccaggttaa gaatgggaaa
     2521 acgccgttat ggtaataaaa ttgtaaattc atggaaaacg aaacggcacg atacgttaag
     2581 taattgagaa aattgtagtc gtaacggcaa gaaatgctcc acatttgaga aaataatgat
     2641 taccattccc atttataaca agagcgtaac gatgattacg cttagcgaag cattgtgaag
     2701 cagcaaaaat atcggttcat caaagggagt cgtcatgcat ttacgtcatc tgttttcatc
     2761 gcgcctgcgt ggttcattac tgttaggttc attgcttgtt gcttcatcat tcagtacgca
     2821 ggccgcagaa gaaatgctgc gtaaagcggt aggtaaaggt gcctacgaaa tggcttatag
     2881 ccagcaagaa aacgcgctgt ggctcgccac ttcgcaaagc cgcaaactgg ataaaggcgg
     2941 cgtggtttat cgtcttgatc cggtcactct ggaagtgacg caggcgatcc ataacgatct
     3001 caagccgttt ggtgtcacca tcaataacac gactcagaca ctgtggtttg gtaacactgt
     3061 aaacagcgcg gtcacggcga tagatgccaa aacgggcgag gtgaaaggcc gtctggtgct
     3121 ggatgatcgt aagcgtacgg aagaggtgcg cccgctgcaa ccgcgtgagc tggtagctga
     3181 cgatgccacg aacaccgttt acatcagtgg tattggtaaa gagagcgtga tttgggtcgt
     3241 tgatggcgag aatatcaaac tgaaaaccgc catccagaac accggtaaaa tgagtaccgg
     3301 tctggcgctt gatagcaaag gcaaacgtct ttacaccact aacgctgacg gcgaattgat
     3361 taccatcgac accgccgaca ataaaatcct cagccgtaaa aagctgctgg atgacggcaa
     3421 agagcacttc tttatcaaca ttagccttga taccgctagg cagcgtgcat ttatcaccga
     3481 ttctaaagcc gcagaagtgt tagtggtcga tacccgtaat ggcaatattc tggcgaaggt
     3541 tgcagcaccg gaatcactgg ctgtgctgtt taacccagcg cgtaatgaag cctacgtaac
     3601 gcatcgtcag gcaggtaaag tcagtgtgat tgacgcgaaa agctataaag tagtgaaaac
     3661 gttcgatacg ccgactcatc caaacagcct ggcgctgtct gccgatggca aaacgctgta
     3721 tgtcagtgtg aaacaaaaat ccactaaaca gcaggaagct acccagccag acgatgtgat
     3781 tcgtattgcg ctgtaataga tatggcgagg gtgaaaacgg agagacttgc ctgactttta
     3841 tcacgccgca tgcggcacac cttcgtcagt aatctaaatt tgcccggcac gtagccgggc
     3901 aattttgcaa ttagctcgcc gtttcaggct taaacacaat ttcctgtttt tcttcatctt
     3961 cctcgacgac tggcgcagtg ctgtgaattt cagcaacgcg tttgcgcaca ccaaaccagc
     4021 ctataaccag cagaataccg ataatcggca gcgccgcgat agtataagtc ccgttcgggt
     4081 aatcgaacgc catcagcaca aggacactca gtaaaaacag taatgtcagc caggaagtga
     4141 agggcgcgcc aggcagttta aaactgacat ccgctgcttt gccttcttta atcgctttac
     4201 gcaggcgcat ctggcacacg atgataaacg cccatgaagc gatgattccc agcgacgcga
     4261 agttcaacac aatctcaaat acgcgcgacg gcaccagata gttgaggaat acgccgacga
     4321 catacacaac cagtgtcgcc agaatcccgg catacggcac atgctgacga ctcattttcg
     4381 ccataaaact cggtgcggaa ccgcccatcg ccatcgagcg cagaatacgc ccggtgcagt
     4441 acagacctga gttcaggctg gagagggcag cggtcagcac cacaatgttc ataatgctgc
     4501 cgatatatgg cacacccagt ttagagaaaa acgtcacgaa cggactttgc cccgcctgat
     4561 acgcgctcca cggcaataac ataaccagca acaccacgga gccgacgtaa aacaggccaa
     4621 tacgccaaat cacactgtta atggctttag gcaccatggt ctgcggatct ttacattctc
     4681 cggcagctgt acccaccatt tcaatggagg caaaagcaaa cactacgccc tgaatcaaca
     4741 ccagcgcagg cagcaaaccg tgggggaaga agccgccatt atcggtgatt aaatgaaagc
     4801 cagtggtgtt gccatccagc ggctgaccac taccgaggaa cactgtaccc acgaccaaaa
     4861 aggtcacaat ggcgagcact ttaataagcg caaaccagaa ctccatctcc gcaaaccatt
     4921 tcacaccgat catattcatg gtgccaacga tggtaagtgc agcgagcgca aagacccact
     4981 gcggcacgcc gccaaacgca ccccagtaat gcatatacag agcgacggcg gtaatatcaa
     5041 caatccccgt catcgcccag ttgatgaagt acatccagcc agcaacataa gcggctttct
     5101 cacccaaaaa ctcacgggca taagagacaa aactgccact ggaagggcgg tgtagcacca
     5161 gctcacccaa tgcacgcaga ataaaaaacg aaaacaagcc acaaattaaa taaaccagtg
     5221 ccagtgctgg acccgccatt tgcagtcggg ctcctgcacc taaaaacaag ccggtgccaa
     5281 tcgcgccgcc aatggcgatc atctgtacct ggcgattgcc catcgctttg tgatacccct
     5341 cttcgtgggc attaagccag cggcgtttcg cggcgtgttg atctgaagtg tcggtgtcgt
     5401 gtttactcat tgctctccct gattgcttta atgaaaaagt catataagtt gccatgaaca
     5461 atgtttattc atcctggcga tgatagttat caccattatt ctttatgggt ataaaactat
     5521 cggcgcagca tcctacccgt tatcgatata cgatgcaaaa catcccctta caatcctgaa
     5581 ggggattcat acaactgacg aaaaaatgac aaatactttt gctggttaac ctgtgtactg
     5641 tcctacactt aatctttaaa agattgtgag gggcatatga ttaaggtgta tggcgtaccc
     5701 ggatggggct ctacaatcag tgagctgatg ctaacactgg ctgatattcc ttatcagttc
     5761 gttgatgtca gtggttttga ccacgaggga acttcacgcg agttattgaa aaccctgaac
     5821 ccgttgtgtc agattccgac actggcgctg gaaaatgacg agattatgac ggagacagcg
     5881 gcgattgcat tgatggttct cgatcgtcgc ccggaccttg cgccaccggt tgggcgcgcc
     5941 gaacgtcagc agtttcaacg gctattggtc tggctggttg ctaatgtcta tccaacattc
     6001 actttcgccg attaccctga gcgttgggct cctgacgcac ctgaacagtt aaagaagaat
     6061 gttattgaat atcgtaaatc gctttatatc tggctgaatt cacaattgac cgctgaacct
     6121 tatgcgtttg gtgagcaact aacgctggtg gactgttatc tttgcactat gcgcacatgg
     6181 gggtctgggc atgaatggtt tcaggataat gccacgaata tcagtgcgat tgctgatgct
     6241 gtatgtcagt taccgaaatt acaagaagta ttaaaaagga atgaaattat ttgagtggga
     6301 taccagagga ttatgttatt tatta
//
LOCUS       DAZPJR010000210         6305 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPJR010000210 DAZPJR010000000
VERSION     DAZPJR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.215"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..277)
                     /locus_tag="ACHVB7_003944"
     CDS             complement(<1..277)
                     /locus_tag="ACHVB7_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915803.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVS"
     gene            complement(336..4193)
                     /gene="sigA"
                     /locus_tag="ACHVB7_003945"
     CDS             complement(336..4193)
                     /gene="sigA"
                     /locus_tag="ACHVB7_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR1915804.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4554..5558)
                     /locus_tag="ACHVB7_003946"
                     /pseudo
     CDS             complement(4554..5558)
                     /locus_tag="ACHVB7_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5543..5920
                     /locus_tag="ACHVB7_003947"
                     /pseudo
     CDS             5543..5920
                     /locus_tag="ACHVB7_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5959..>6305)
                     /locus_tag="ACHVB7_003948"
     CDS             complement(5959..>6305)
                     /locus_tag="ACHVB7_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1915805.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1572 a         1408 c         1411 g         1914 t
ORIGIN      
        1 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
       61 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      121 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      181 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      241 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      301 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      361 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
      421 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
      481 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
      541 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
      601 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
      661 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
      721 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
      781 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
      841 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
      901 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
      961 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1021 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1081 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1141 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1201 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1261 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1321 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1381 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     1441 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     1501 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     1561 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     1621 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     1681 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     1741 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     1801 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     1861 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     1921 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     1981 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2041 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2101 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2161 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2221 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2281 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2341 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     2401 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     2461 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     2521 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     2581 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     2641 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     2701 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     2761 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     2821 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     2881 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     2941 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3001 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3061 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3121 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3181 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3241 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3301 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3361 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     3421 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     3481 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     3541 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     3601 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     3661 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     3721 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     3781 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     3841 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     3901 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     3961 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4021 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4081 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4141 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4201 ctccgatatt tctgagttgt ggttatggga taacatttaa ttacaacggg gaactatcag
     4261 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4321 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4381 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     4441 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     4501 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     4561 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     4621 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     4681 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     4741 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     4801 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     4861 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     4921 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     4981 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5041 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5101 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5161 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5221 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5281 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5341 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     5401 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     5461 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     5521 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     5581 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     5641 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     5701 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     5761 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     5821 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     5881 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     5941 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6001 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6061 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6121 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6181 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6241 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6301 cacca
//
LOCUS       DAZPJR010000211         6256 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPJR010000211 DAZPJR010000000
VERSION     DAZPJR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.216"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHVB7_003949"
     CDS             122..952
                     /locus_tag="ACHVB7_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR1915806.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHVB7_003950"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHVB7_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR1915807.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHVB7_003951"
     CDS             complement(2081..3541)
                     /locus_tag="ACHVB7_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR1915808.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHVB7_003952"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHVB7_003952"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR1915809.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..>6256
                     /gene="gntP"
                     /locus_tag="ACHVB7_003953"
     CDS             5146..>6256
                     /gene="gntP"
                     /locus_tag="ACHVB7_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR1915810.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISA"
BASE COUNT         1480 a         1619 c         1588 g         1569 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcac caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttaat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctg
//
LOCUS       DAZPJR010000212         6083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPJR010000212 DAZPJR010000000
VERSION     DAZPJR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.217"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHVB7_003954"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHVB7_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1915811.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHVB7_003955"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHVB7_003955"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR1915812.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHVB7_003956"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHVB7_003956"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR1915813.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHVB7_003957"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHVB7_003957"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR1915814.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHVB7_003958"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHVB7_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR1915815.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1444 a         1535 c         1646 g         1458 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaat atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aat
//
LOCUS       DAZPJR010000213         6048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPJR010000213 DAZPJR010000000
VERSION     DAZPJR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.218"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..286
                     /locus_tag="ACHVB7_003959"
     CDS             <1..286
                     /locus_tag="ACHVB7_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1915816.1"
                     /translation="RQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFR
                     CQVCGYTVNADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(326..997)
                     /locus_tag="ACHVB7_003960"
     CDS             complement(326..997)
                     /locus_tag="ACHVB7_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR1915817.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1104..1337)
                     /gene="yedF"
                     /locus_tag="ACHVB7_003961"
     CDS             complement(1104..1337)
                     /gene="yedF"
                     /locus_tag="ACHVB7_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR1915818.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1334..2539)
                     /gene="yedE"
                     /locus_tag="ACHVB7_003962"
     CDS             complement(1334..2539)
                     /gene="yedE"
                     /locus_tag="ACHVB7_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR1915819.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2726..3139
                     /gene="yedD"
                     /locus_tag="ACHVB7_003963"
     CDS             2726..3139
                     /gene="yedD"
                     /locus_tag="ACHVB7_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR1915820.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3173..4660)
                     /gene="amyA"
                     /locus_tag="ACHVB7_003964"
     CDS             complement(3173..4660)
                     /gene="amyA"
                     /locus_tag="ACHVB7_003964"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1915821.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4738..5103)
                     /gene="fliT"
                     /locus_tag="ACHVB7_003965"
     CDS             complement(4738..5103)
                     /gene="fliT"
                     /locus_tag="ACHVB7_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR1915822.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5103..5513)
                     /gene="fliS"
                     /locus_tag="ACHVB7_003966"
     CDS             complement(5103..5513)
                     /gene="fliS"
                     /locus_tag="ACHVB7_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR1915823.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5528..>6048)
                     /gene="fliD"
                     /locus_tag="ACHVB7_003967"
     CDS             complement(5528..>6048)
                     /gene="fliD"
                     /locus_tag="ACHVB7_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1915824.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1479 a         1593 c         1503 g         1473 t
ORIGIN      
        1 ccgccagctt gagtacaagc agctctggcg tggcggtcag gtgcttgctg ttccgccagc
       61 gtacacaagc cagcgttgcg cgtgctgtgg tcatacagcg aaagaaaacc gcctgtcaca
      121 aagtaaattc agatgccagg tatgtggata tacagtgaac gccgatgtaa atggcgctcg
      181 taacatttta gcggcggggc acgccgttct tgcctgtgga gagatggtgc agtcaggccg
      241 cccgttgaag caggaaccca ccgaaatgat tcaggcgaca gcctgaacgt agcagggatc
      301 ctcgtccttc agggcgtgga ggatgtcaac aaacaggttg aattaactcc gcgccctggt
      361 ttttgacatt acctaccgcg cgcgataccg ggtgccaggt gaactggttt gctggcacac
      421 agccgttggt agcaatttct gatgcttctt taccgccaat ctcttgccgc atccattcct
      481 gtgcagcttc tggcgacaga accagcgggc ggcggtcatg aatgtctacc agaccctggt
      541 ctgctgcagc agtgacaatt aaaaatcctt cggcttcgtc accacgctca aatggtgtgc
      601 tacctatcgc ggccataaaa ataggttgtc catcagcacg atagataaaa aaaggctgtt
      661 ttttgtcgcc ttcttttttc cactcaaacc agccatcggc aaaacagatt gcccgaccat
      721 gttgccagag cggtttaaac atgcgactgg ttgcggcagt ttctacgcgg gcgttaatca
      781 gcggcggttt atcccaccat ccgggagcat atccccagaa aaccggatcc agatgaaggt
      841 gttcatcacg ttcactgagc agaagaactt tggttcccgg cgcgacgttg tatctgccaa
      901 tgggttcggg atcgtaggga atatcgcgtt caatatcttc cgcgagaagc gcaaggtaat
      961 cttcacgcgt ttgggattgg gcaaagcgtc cacacataga aacctcctga gatcatactg
     1021 aaaagaatag gagagggggc gggtaaaatc tatgtgtcag aggcaacaat gttcccgctg
     1081 tgggccacag cgggaatgag ggattacttc tgaattaaat aacgaatggt cggcccgtct
     1141 tgctgaatat ccagcaccgt atagccgtga ttacgcgcat ccagtggaat attattgatc
     1201 gactgtggac agtcgctcac cacttccagg atttcccctt tttttaactg cggcatcgcc
     1261 tcaagggttg cgactgccgg ataagggcag ggttcaccca ccatatcaag gcggtaatca
     1321 ggaacgatat ttttcatgcg atctccttag cagtctgcgg tgccgcacgg cggaagaagc
     1381 gtttttccca gccgataatc aacatcagcg cagtaaacaa cagcaaatat gtcaccagca
     1441 ggccacccat cgggccaaag gttttcagca ggttgatttt gtcccagtcg gtggccagcg
     1501 ccggagcgaa atcatcccag taatacgcca gaatcgtcga gccgatcaca ttgcccagac
     1561 cgacccacca gtagtgcacc tggccttcta ccgcgcggta catccagccg gtttcgcagc
     1621 cgcctgccag cacgatgcca aaaccaaaca gtaaaccacc aattaccgcg tttggtcccg
     1681 cccacatgat tttgggttca acgcctaact gtacgtaact gaagatcccg atggcactca
     1741 ccgccatacc gataatgatt gctttcgcca tatgggtacg tccggtgatc cacatatcgc
     1801 ggaacgccga agtaaagcag atttgcgcac gttcaatcag taaaccaaag ccgacgccaa
     1861 acagcattgc cagccccagt ttgggttggt tcatcgccgt gagcagcgcc cagcccagca
     1921 tgccgaaaaa gaccagcatc ccgagacgaa aacgacgccg cgcctgatcc ggtttttgcg
     1981 tcagcggtga ggcggcagaa actttctgca ttttcacggg aatacggaag atgggcagca
     2041 gggtaaagcg cgcgccaaac catgaaccaa tggcagtggc gatggcaaag aaccaggcat
     2101 gcagcgagaa ctgaggaatg ccggtaaaga acgccgccag gttacagccc attgccagac
     2161 gcgcgccaaa accggcgata atgccgccaa taatggcctg cataatgcgg atacggctgc
     2221 gcggcatgcg cagtttgaca ttgttggccc acagcgctgc ggcaaagcag ccgccaaaca
     2281 tgccgaggat catcatcccg tcgatgcggg ttaatggcga tccttccaga tggataattt
     2341 taaaataacc ccactcttca gcatggacgc caaacagctg caggagctga ccgccccaac
     2401 gggtaaattc acccgtgaca gcccaaaagg tgccagtaat gccaaaatag taagtggaga
     2461 gaatacccgc cgcgatgacc gcagggatgg gtgcccagaa tttaatcaac caggcgtgtt
     2521 tgaattgctg ccatgacatg aatgtagcct ctgaactcag aaggtttgaa acaagaatcg
     2581 cgaatcatac atcatgcaaa tttattttcg gggtaataat tcacaaaaat aatagttaga
     2641 tcaaatttct ctctgaacct ggaaaggctg gcgacatcag ggctttaatg ccacaatcct
     2701 cttttcttct catgcaggac gaatgatgaa aaaactcgca attgcaggtg cgctcatgtt
     2761 gctggcgggc tgtgccgaag tcgaaaacta taacaatgtc gtgaaaaccc ccgcgccgga
     2821 ctggctggcg ggctactggc agaccaaagg gccgcaacgt gcgctggtca gtccggaaga
     2881 gataggcagc ctgattgtca ctaaagaggg cgataccctg gattgccgtc agtggcagcg
     2941 ggtgattgcc gtgccgggca agttgacatt gatgtctgat gatctcacta acgttaccgt
     3001 aaaacgtgag ctgtacgagg ttgagcgtga cggtaacacc attgagtacg atggtatgac
     3061 gatggagcgt gtggatcgcc cgacggcaga gtgtgccgcc gcgctggata aagcgccgtt
     3121 accgacgcca ctgccgtaat gaaatctccc cggcttgccg ccggggatga atttaaatca
     3181 cctcttcgat aacccacacg ctgacgctgc cgccgttgca aaagaaggtc gcttcgccgt
     3241 tttcgtcggt cactacgctc tcttgccgat tccccagaaa atcacgccag gttttattgc
     3301 cgtaattctc tcccagatga atggtttttt cgccatcatc cccgttcgac atgaccacca
     3361 cgcagccggg atattcgtca gtgccactgc ggctaaaggc aatgcagttc ggatggtcga
     3421 aaaataacgt ctgtacaccg tgggcgaaac gctgacgggc gagaattaac tcatcaagct
     3481 gttcgattat tggcatatct atcggatagg tttgcccgtc accaccgaca tcttcgtaat
     3541 gcgcaccgta gaggtccgga tagaacaccg aaggaacgcc attttcccgc aacaaaatta
     3601 gagcatacgc cagcggttta aaccacggtt cgaccggcgc ttcgagggct tgcaacggtt
     3661 gggtgtcgtg attggctacg agggttacgg cgtggaaagg atcggcttcc actaatgtac
     3721 ccgtgaaaat ctgcgtcatg tcgtagtcgc gtcccatgcg cgatgcttca tggaatttca
     3781 tctgcagcgg cgcatcgaac agcatggttt tgccttccac ctgatcaata tacgtttgca
     3841 gtttatcgac ttcatgcgac cagtactctg ccacaataaa cagcggcttt ggcgcaactt
     3901 cctgtacgtg ttcgatccac tctttataaa accacgccgg aatatgttta accgcatcaa
     3961 gacgaaaacc gtcgcattgt gtttgttcca tcacccagcg cgcccagtat ttaatctctt
     4021 ccgtcacggc atgattgcgg aaatcgatat tctcacccat cagataatcg aaattaccga
     4081 gttcatcatc aacctgatcg ttccagcctt cgccggtgta gtcgttaaca attttaaata
     4141 tgccatcttc atcaggattt tcgatatggt cgataccgct aaaacatttg aagtcccaga
     4201 taaactgcga gtattgcccg gcacgggcgg ggaaggtgta acgcgtccag ccttcgcact
     4261 caatgatttc ttcatcaatt tgcgtacggt catcagcatt tacacgctgc acgcgaatag
     4321 cttctttttc atccgccccc attttgtggt tgactaccac gtccagtagc accgcaatgt
     4381 cattacgttt cagagcgtca atggcggcca gcagttgtgc tttatcgcca tatttagtag
     4441 ggatgctgcc tttctgatca aactcaccta aatcaaataa atcataggag tcgtagccga
     4501 ccgaataccc gcccgatgcg cctttatagg cgggcggcaa ccagaccata ttgataccaa
     4561 tatcattaaa accgtcggcg cgctcggcca gttcaggcca gagcttaccg ccttccgggt
     4621 aataccagtg aaaacattgt aacagcgtgg gattacgcat tcttcgactc cattcaaggg
     4681 gaacattaga agcgtagccg taatcggatt attcgcgagc catcggctcg ttaagattca
     4741 aaagaggtta tcctgcggag ccagcacaaa gccgccctga tctccatagg cacttaacac
     4801 tgatttttgc accgatgact gaccgaccag tttcgccagt tcatccatac gaatctgtaa
     4861 taactgcttt accttgcttt cgttgtcgag aatcaggcgc agcatcgggc ggagctgctc
     4921 ctgcatcgtg gtggacgggg caacctcttc agtcagatgt gcaatctcct gcaccgcatt
     4981 cacatacgcc atttcgctgg cgatgagttc gtcccattgt tcttccgttg ccaggcgtaa
     5041 catgagctgg cttttatcga cgagttgttg ccaggcgaaa tacaaatgcg gtgcatggtt
     5101 catcagactg ggtcctgaat caaagaaggg gagagtaaag actctttcca ggcatcggca
     5161 atattgcgca ttaatgcttc cacttcttcg actgcggaga catcgttgcg taaattggct
     5221 tgcagcaagc gcctgaccat atagctataa agagcaatca agttttgggt tagttcgtct
     5281 ttgctctctt catcaagact cacccgcagc ccgttctcaa tgatgttgat cgcttttgac
     5341 aaagagacgc ctttgccttg ctgattgttg tcctgcataa acaggcgcgc tctaaccagt
     5401 gcgctcagca ctccatcaaa tagcatggtg accagctgtt gctggctggc gctcattacg
     5461 gcgctttcga cgccaatttg tgcataggct tttgagccgc tcgcggtata catgctgacc
     5521 tccgtgatca ggaattactc attgcagtaa attgttggct gagataggta ctggtgttat
     5581 tcagtttgct catcatggta tctaattgcg taaactgtgc agtgtaacgc gccacggtgt
     5641 catcaatgct ggcactgacg gatagatact gtttggtcag ttttttcagt gtggcgttaa
     5701 tactgtcctg tgcgctatca ataatgccat catcggcaag gtaacctttt acctcggtgg
     5761 caattttggt ggtaattcct gtttctttac catcgcctac cagcaattca cgtacggatg
     5821 ctgtattttc attcaggact tttttgagtt tatcatcatc aattttcagc ttacccgtcg
     5881 tgccgtcctg cttaatcccg atctcgttta atgttttgaa ggcactgtcg ctggcaccgt
     5941 ttgcaaattg tgcgcggatg ccgctttgaa ttgtacgtac aacgctgtct ccgagaagtg
     6001 caccattatt tttatcttgt gcttcagcac ctgggtctac ttctttat
//
LOCUS       DAZPJR010000214         6015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPJR010000214 DAZPJR010000000
VERSION     DAZPJR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.219"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(369..1304)
                     /locus_tag="ACHVB7_003968"
     CDS             complement(369..1304)
                     /locus_tag="ACHVB7_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1915825.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     TSARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
     gene            complement(1802..1945)
                     /locus_tag="ACHVB7_003969"
     CDS             complement(1802..1945)
                     /locus_tag="ACHVB7_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFR1915826.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGV
                     ETQ"
     gene            complement(2029..2286)
                     /locus_tag="ACHVB7_003970"
     CDS             complement(2029..2286)
                     /locus_tag="ACHVB7_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="HFR1915827.1"
                     /translation="MGLKLHIHWFDKKNEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVEKRWVPILQPYFKNVIDISKFDYFVSFDYRDGDW"
     gene            complement(2296..3933)
                     /locus_tag="ACHVB7_003971"
     CDS             complement(2296..3933)
                     /locus_tag="ACHVB7_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HFR1915828.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVS
                     GEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTN
                     VQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASF
                     PGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKP
                     VYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQ
                     RAQTDVNNKKAAFDAAAKEKSAADAALSSAMESRKKKEDKKRSAENKLNEEKNKRRKG
                     TKDYGHDYHPAPKTEDIKGLGELKEGRPKTPKQGGGGKRARWYGDKGRKIYEWDSQHG
                     ELEGYRASDGQHLGSFDPKTGKQLKGPDPKRSIKKYL"
     gene            complement(4154..4687)
                     /locus_tag="ACHVB7_003972"
     CDS             complement(4154..4687)
                     /locus_tag="ACHVB7_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915829.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(4690..5616)
                     /locus_tag="ACHVB7_003973"
     CDS             complement(4690..5616)
                     /locus_tag="ACHVB7_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR1915830.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
     gene            5792..>6015
                     /locus_tag="ACHVB7_003974"
     CDS             5792..>6015
                     /locus_tag="ACHVB7_003974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915831.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEG"
BASE COUNT         1487 a         1660 c         1297 g         1571 t
ORIGIN      
        1 aaaaaaacaa cgacgagaac gcaatcaccg actctttctc gcggctgggc tactgatcga
       61 agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca
      121 ggcacttaag gaaattaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc
      181 tccatccgtg ttcctgttcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat
      241 gagactgcgg cgagcactac cagcacaatc atgcggaaaa cagcaccatt gcagggctga
      301 acggggaaaa acggcaaaag acctaaggct caaaaaaacg ccagaaaggc gctgttatct
      361 gatataggct attttccgct tttccagttt cgaatggttc gagtagaaac ctccaacatc
      421 gccgcaatag ctttctgaga atatccattc tccagcatac atagcgccat gaaacgcttg
      481 tcatcgtagg cttcgccttt agcgatgcca cctttcctgc ctctcttggc ctgtatttct
      541 ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg ccgtgaagtt cctgtgcgtg
      601 tatttcgcaa tactcttgcc aatagcccga cattcaggag gtgaaagcgg aacgggaagc
      661 gatgcgttgt acatttcgac acgctgaatc acggcatcaa gccactgtga gaatgctggc
      721 cagccctgac gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag atggcagttt
      781 ctccccattc cgtaatgttt atcgatgcta cggcgcgctg aagtgctcaa atcgagataa
      841 tcagccagtt catcgagggt ataggcttcc tcacgccact ccatcacctg ccattccaga
      901 tggaacggat ttttgcaaat cagtccgctg tagtttacat ctgcgcccag tttttcacac
      961 aacgcacgtt caatcgcggc ggcgtatttc agcgccttaa ccgaagcatc aggcgcggtt
     1021 ctcacggcga tattgagtgc gtagagcagg tgggcgtgac cgttcacggg attttttatg
     1081 gtgatgttcg gtgcgggggc gttcaggtcg ctccagtcaa tcgcagcccc ctcgcggtca
     1141 acgtcaaaaa ccagccagaa ctgcgcatga ggttggttct gctggatata ccgtgcaaga
     1201 agcgcgcgcc ctttaccgga gatacgaagg ccgaaagcga gatcatccgt gtgatagggg
     1261 cggaggggta aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc
     1321 caggttatcg accgacctcg cccctgtatt ctcgtgacaa tcttggcgga gagacacgaa
     1381 tttcaggggc gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgatc
     1441 cttcccgaaa acccgatcca tttaccataa ctcccgttgt acgtacccct taaaacgccc
     1501 tcaatcgctt tctggcgcaa aaaacgcaaa aagacaagaa aacaccagga caaaccagaa
     1561 aaacgcgtca gaatcgtttt cagggcgttg cataactatg cacgaaagcg atgtgcgatc
     1621 ggtttcgtgc gttctgaaca ccgaacggct caaaacgaaa agttttctcc ctccggatgg
     1681 aaggttctgc acggagattt tagatcccat ctcccacatg gaatttttgt ggcacttcaa
     1741 caaaaaaagc gcgtcagaat cagcctgaca cgctttgtcc ttgtcaaatg aggattgcag
     1801 gttactgcgt ttccactccg gtcagttcag cagaggatga cggtgataca gtcccgccct
     1861 gaacatcacg gatatagttt gcctgacatg cagcaagaat aatggctgca agaagcaaca
     1921 aaataatccc tgttattttc ttcatacgat tccctgtata tatggattca ggaattcatc
     1981 cttgaacacc tgaatcaaac actttattac tcgtccctga tagtttaatt accagtcacc
     2041 atcacgataa tcgaaagaaa caaagtaatc gaacttacta atatcaataa catttttaaa
     2101 gtatggctgt aatattggca cccatctctt ttcaacatca aaccaaccgt tattaatatt
     2161 atcctttaaa ggcattccaa gacgttcaat aaccgaacca tcatcaccga aatcctttga
     2221 atactcacca cctttaaatt cctcattctt cttatcaaac caatgaatgt gtaatttaag
     2281 tcccataaca tcaccttaaa gatacttttt aatacttcgt ttcggatctg gacctttcaa
     2341 ctgcttaccg gttttagggt caaatgaacc aagatgctga ccatcactgg ctcgatagcc
     2401 ctcaagctca ccatgctgag agtcccactc ataaatttta cgccccttat ctccatacca
     2461 tctggcacgc ttacctccac caccttgctt tggagttttt ggcctcccct cttttaattc
     2521 ccccaaacct ttaatatcct cagttttagg agctggatga taatcatggc cataatcttt
     2581 agtgcctttt cggcgtttat ttttctcttc gtttaattta ttttcagcac tccttttctt
     2641 atcttctttc ttcttcctgc tttccatcgc agaactcaat gcagcatctg ctgctgactt
     2701 ctcttttgcg gcagcatcaa atgcagcctt cttattattt acatccgtct gtgcccgctg
     2761 ggccttaagc cctgccattt gccacattct gtgaccagca gccattggtt ctcgggcaaa
     2821 tcgctcgaat tgttttattt cagcctttgc atcagcaaga gttttattcg ctgcatcaag
     2881 ttcactttta cgagaattat aaacctgaac agctttagcc tgtcgttcct ggtttctggc
     2941 aacatcttta ttagcctgat tcagttctgc gcgggcctgt tcataattgc gttcagccac
     3001 ttccaccgga tgtgcgtcat tccattcctg ctggaggcgt ttttcctcat cctgacgttg
     3061 ttttacctgt gctggggtaa gaacatctgt cactgataca taaaccggct tctgcccact
     3121 ttctttcgga aagcgaatca ccgcttcatg tgaaccacca ccaaaggtaa atcccgcagg
     3181 aacggcagtc cggcctttat cctccgtaat accacgggga agggttgttg acaccggaag
     3241 gcctttgaca gtgctgactg tcagagaagg gacaccagga aatgatgcgt gaaatacacc
     3301 tggagtacgt gttggtttgg cattcacaac aggcacactc ataggcacac ctgcaactac
     3361 ggcaatatgc tgtcgtgtgt ccttcacaac atctgtcaca cgtttcgtca cgctgacagt
     3421 tgcctggtcc agcggaagtg tactgacctg aacattcgtc actgtttctg ccggtaatga
     3481 cgtcacaatc tttgacatca tattcgggtc atcttttgct atttcagatg gcagaatgcc
     3541 gtaaagcgca ataccccatg cgctgaattt aaacgggcct ttcagggctg caaagatatc
     3601 cgctattgcc gcagataaag cctcaccaga gacggcgata cccagcgttc cggcaccagg
     3661 agttgccaaa gcaggaaaac caaatgccac tgctgctacg gatgaattgc tgccaccacc
     3721 ggaattacca ttccccccgc cattaccatt gccggagcca cctccccagt gaacaccact
     3781 accggaaccg ccaccccatg ggttattttc cgaactccat cctgaaccgt cagaagcccc
     3841 accatttcca ccaagccccg tagggccgcc attaatgtta ccacctgtgt tatgtgcgcc
     3901 actgttgtgg tcgcgtccat ctccaccgct cataaaaatt ccctctttta agcgttaaaa
     3961 caattgatta tatagttgat actgtacata aaaccactgg ttttatgtac agaccaaaaa
     4021 gtactcgcca ttattttttc tgtcaagtca gggggggata ttttttggca gttcggagga
     4081 atcactcatg aaaacggtga acggaacgcg aaatcctttc gcgggtcgtg aggcgttgga
     4141 caagcgaagc gcgtcagttt ttccggctct tacccgtgac aggtttctcc accagtttat
     4201 cttccagtgt cacaactttc tgatacaccg ccgctgtggc gttgtagttc ctgtcgagaa
     4261 ttgttttcat cccccatccg agcacactga acataaccag aaaggccact gacaccccac
     4321 ccagccattt ccagctatac cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa
     4381 ttgtcgcaat atccctgttc agggcatccg cgtttgcctt catgtttgcg actgactctt
     4441 ccagccgctg aaaatcctga ctgatgcgct cacctatcag ccctgcaaca tccagcctct
     4501 gcacttcgtt tctgacttca tccctgacct gttttgccac accgtcagaa atatcccgat
     4561 atccgcgaag tgtttccctt gccacagcca gagaatcagc aacagctttc tgttgctcca
     4621 ttgcggtatg ttgcagatgc tccaccgata acaggagcgt gagtaatttt tcttctgtgc
     4681 tggccatgtt cagaatccca tagaaaatcc acgtgacggt gatttaaccc gttcaggttc
     4741 agttcggttc tgtctgctca ctctgtctct catgtcaaag ccatgctccc gcgcaacatc
     4801 atgttgcagc cttctgacca gttcatgttg cttaaccgga gtgtccagcc attccctgat
     4861 gtcatgcccg accgttcgcg cctgttcaag atggcgggaa tagtccggtt cagaccgttt
     4921 ttgcatgatg tcatgacgct gggcatcatc aagccgtccg gcagcttcaa aacccagatg
     4981 cacttcggga cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct
     5041 ttcaaagcca tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc
     5101 ccgcaactgc tctttttgct gagtgagagt caggcttccg gaatcctttt tcgcacctcc
     5161 ccgctcggga tacctggcgt tatagcgccg gaaatactgt tccggtgtac gttcaatgcc
     5221 atcattcaca cgctggctca tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct
     5281 cgctttcgga ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt cctgacgtac
     5341 aaaatcctgc accagttcaa ggcgctgttc cgctgtcagt tcacggggta atgcaatttc
     5401 aagttcccga taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg
     5461 tgagggatta tctcgcgccc attccggcat gtttccgtgt gcggaatatt ccaaatcctg
     5521 acggtcgcgg tatttctctt tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt
     5581 gccaccaaat ttaacgctga ggtgataggt tgccacggtt tcagccctcc gcaggagcgc
     5641 actgtttctc gacgaaggag agaaacatat aagtgcgctt ccttctaatt ttacgaatta
     5701 gcacggaaga taccccctgc acccaccgac cgcaagcgat atactgcacg accacaacac
     5761 acagcaaaaa acaccaggga gcaccacgaa catgcgcatc agcaacatcg aatggctgaa
     5821 gaaacggatc ggattcatca ggaagctcgg agaacagacc gcacgacaga gacagattat
     5881 tgacctgctc gacaacgaag caggactcac agaacaggaa cgcaaactcc tccacgtact
     5941 ggcaacagcg gaaaaaaacg acttacaggc acaggagagc gaacgaaaac aggcggtcca
     6001 aaaacgaatc gaggg
//
LOCUS       DAZPJR010000215         5961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPJR010000215 DAZPJR010000000
VERSION     DAZPJR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.220"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHVB7_003975"
     tRNA            292..368
                     /locus_tag="ACHVB7_003975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHVB7_003976"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHVB7_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR1915832.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHVB7_003977"
     CDS             complement(1357..2247)
                     /locus_tag="ACHVB7_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR1915833.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHVB7_003978"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHVB7_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR1915834.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="ACHVB7_003979"
                     /pseudo
     CDS             complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="ACHVB7_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1354 a         1564 c         1556 g         1487 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttcgg
     5941 taatgactcc aacttattga t
//
LOCUS       DAZPJR010000216         5952 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPJR010000216 DAZPJR010000000
VERSION     DAZPJR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.221"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1484
                     /gene="tssI"
                     /locus_tag="ACHVB7_003980"
     CDS             <1..1484
                     /gene="tssI"
                     /locus_tag="ACHVB7_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR1915835.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRV
                     GLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGT
                     KIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            1552..5547
                     /locus_tag="ACHVB7_003981"
                     /pseudo
     CDS             1552..5547
                     /locus_tag="ACHVB7_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1425 a         1507 c         1919 g         1101 t
ORIGIN      
        1 tctgcatcag ccagtttcgc tacagcgcac aaatccgtcc ttcttccgta gtgaccaaag
       61 actacacctt taaacgtccc ggctggccag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag ggtgcccacg
      181 ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcgagcga tcttcacggc gaacagccgc
      361 aggcggtacc gggacgcagg ggttcaggca ccacgctgga gaaccatttt gcggtaatcc
      421 cggcagacag aacatggcgg ccacagccgt tgatgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc
      661 aggaggtgat tgtggacttc ctcaacggcg acccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggca gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgctg aaaccattgg caacaatcag atgattgcgg
      961 ttaccaacaa tcagatagag acggtgggcg ttaaccagat agagacggtg ggcagtaacc
     1021 agataattaa agtgggttct gttcaggtgg aaacgattgg acttgttcgt gcgctgacgg
     1081 tgggcgtggc gtatcagacg acggtaggtg gcattatgaa cacctcggtg gcactgatgc
     1141 agtcctcgca gatcggtttg cataaatcgt tgagggtcgg gctgggttat gacgtcaaag
     1201 tcggaaataa cgttaccttc accgttggta aaacgaaaaa ggatgatacc gggcagaccg
     1261 cgatttactc cgccggtgag catctggagc tctgctgtgg taaggcaagg ctggtgctga
     1321 cgaaggacgg acaaattttt ctcaacggca caaaaattca tttgcagggt aaggagcagg
     1381 ttaatggtga ctcactgttg attaactgga actgtgcagc ctcgaaatcc ccaccgaaga
     1441 cccctgatga aaagcaggat acgccggata tgagagagta ctgatcccgt acctgttcgg
     1501 tattcagtaa caaaccagca ttaaaaataa acagaagaag gaatttgaat catgagcgga
     1561 aaaccagcgg cgcgtcaggg agatatgact cagtatggcg gtcccattgt ccagggttcg
     1621 gcaggtgtaa gaattggcgc gcccaccggc gtggcgtgct cggtgtgtcc gggcgggatg
     1681 acttcgggca acccggtaaa tccgctgctg ggggcgaagg tgctgcccgg cgagacggac
     1741 cttgcgctgc ccggcccgct gccgttcatt ctctcccgca cctacagcag ctaccggacg
     1801 aagacgcctg caccggtggg cgttttcggc cccggctgga aagcgccttc tgatatccgc
     1861 ttacagctac gtgatgacgg actgatactc aacgacaacg gcgggcggag cattcacttt
     1921 gagccgctgc tgccggggga ggcggtgtac agccgcagtg agtcaatgtg gctggtgcgc
     1981 ggtggtaagg cagcacagcc ggacggccat acgctggcgc ggctgtgggg ggcgctgcgc
     2041 cggatatccg gttaagcccg catctttacc tggcgaccaa cagcgcacag gggccgtggt
     2101 ggatactggg gggggctgag cgggtgccgg gtgctgagga cgtactgcca gcgccgctgc
     2161 cgccgtaccg ggtgcttacc gggatggcgg acgctgacgt accggcgtga ggccgccggt
     2221 gacctggccg gggaaatcac cgacgtgacg gacggtgccg ggcgggagtt ccgtctggtg
     2281 ctgaccacgc aggcgcagcg tgcggaagag gcccgcacct cttcgctatc ttcttctgac
     2341 agttcccgcc ctctctcagc ctcagcgttc cccgacacac tgcccggtac cgaatacggc
     2401 cccgacaggg gtatccgcct ttcggcggtg tggttgatgc acgacccggc atacccggag
     2461 agcctgcccg ctgcgccact ggtgcggtac acgtatacgg aagccggtga actgctggcg
     2521 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcgcccgggc
     2581 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacg
     2641 gggcgggtgg tggagcaact gaacccggca gggttaagct accgctatct ttatgagcag
     2701 gaccgcatca ccgtcaccga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
     2761 gccgggctga aacgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcgcagcggg
     2821 tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac
     2881 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
     2941 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtacccgga cgggctggag
     3001 agccgccggg aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
     3061 acagtacgct accgctacga tgacgcgcac agtgagttac cggcgacgac aacggatgcg
     3121 acgggcagca cccggcagat gacgtggagc cgctacgggc agttgctggc gttcactgac
     3181 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggttcac
     3241 cgcgaggaag gtatcagcct ttaccgccac tatgacaacc gtggccggtt aacctcggtg
     3301 aaagacgcac agggccgtga aacgcagtat gagtacaacg ccgcaggcga cctgactgcc
     3361 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
     3421 agcaccacgc agggcgggct gacgcgcagt atggagtacg atgctgccgg acgtgtcatc
     3481 agtctgacta acgagaacgg cagtcacagc gacttcagct acgatgcgct ggaccggctg
     3541 gtacagcagg gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa
     3601 ctcacgcaga gtgaggatga ggggcttgtc accctctggc actacgatgc gtcggaccgc
     3661 atcacgcacc ggacggtgaa cggcgacccg gcagagcagt ggcagtatga cggccacggc
     3721 tggctgagag aaatcagcca cctgagcgaa ggccaccgtg tcgctgtcca ctacggctac
     3781 gacgataagg gccgcctgac gggcgaacgg cagacggtgg agaacccgga gacgggggaa
     3841 ctgctgtggc agcatgagac gaaacacgcc tacaacgaac aggggctggc aaaccgcgtc
     3901 acgccggaca gcctgccgcc ggtggagtgg ctgacctacg gcagcggtta cctggcgggc
     3961 atgaagctgg gcgggacgcc gctggtcgag tatacgcggg acaggctgca ccgtgagacg
     4021 gtgcgcaccc tcgcaccgga cctggacatc cgcatcccgt atgccaccga cccggcggga
     4081 aaccgcctgc cggacccgga gctacacccg gacagcacgc tcacggcgtg gccggataac
     4141 cgcatcgcag aggatgcgca ctatgtttac cactatgatg aatacggcag actgacggag
     4201 aagacggacc gcatcccgac gggtgtgata cggacggacg acgagcggac ccaccactac
     4261 gacagccagc accgcctggt gttccacacg cggatacagc atggcgagcc actggtcgag
     4321 agccgctacc tctacgaccc gctgggacgg cgaatggcga aacgggtatg gcggcgggag
     4381 cgtgacctga cggggtggat gtcgctgtcg cgtaaaccgg aggagacatg gtacggctgg
     4441 gacggcgaca ggctgacgac ggtacagacc gacaccacac gtatccagac ggtatacgag
     4501 ccgggaagct tcacgccgct catccgggtc gagacagaga acggcgagcg ggagaaagcg
     4561 cagcgccgca gcctggcgga gaagctgcag caggaaggga gcgaggacgg gcacggcgtg
     4621 gtatttccgg ctgaactggt gcggctgctg gacagactgg aggaagaaat ccgggcagac
     4681 cgcgtgagca gtgaaagccg ggtgtggctt gcgcagtgcg ggctgacggt ggagcaactg
     4741 gccagacagg tggagccgga atacacacca gtgcgaaacg ttcatcttta ccactgcgac
     4801 catcggggcc agccgctggc gctcatcagc gaagacggcg atacggtgtg gagcggggcg
     4861 tatgatgagt ggggcaacca gctaaatgag gagaacccgc atcatgtgta tcagccatac
     4921 cgtctgccgg ggcagcagta tgatgaggag tcggggctgt actacaatcg tcaccggtac
     4981 tatgatccgt tgcagagacg gtacatcacc caggacccca ttgggctgaa aggtgggtgg
     5041 aatctgtatc agtatccgct gaatccggtg agcaggatag atccgttggg gttattctca
     5101 tgtgagagtt gtagtcattt tatagatgat ataaataata aactggataa ccttccgttt
     5161 aacccgacaa aaatgtttac agctagttta aatactgcta attcagcaag gctgtatgca
     5221 agtggcatga caaaattcgg tataagtgtt gctgccgatg gaacgatcat tgctgcccca
     5281 gtcggtgcag cttctatggc tttaggggcg tggaatataa aatctgcgac agctgcatat
     5341 aacagagcaa atttacaatt atcagaatca cttcaggaaa atggtagaga taggtgttgg
     5401 agaaatttat tgggtatttt accttatggt gagcagtttg atgaccctgg tgaaccatca
     5461 atattagacg tttatggtga aaagattgag aatatcaaag attctccaat gaagtttttt
     5521 gaagagcttg ggacgctggg gttgtaatgt gtatttttaa tgatattttt atgtttgtgt
     5581 ttattgttag tagtatactt gtaaccgtgc attttgtgta ttctttattt tttaaaagtg
     5641 gaaaggtaaa agataaggta aaaaaatgga ttaaagtaat gtgtgattta ttttggggga
     5701 taggatagtg gtgtgatatg gataaatata aattacagtg gctggcatat ttcgacctga
     5761 cgccggagta gataaaaaaa cagacaggat tggagtatag caagcgtctg acaatctctc
     5821 gggacagtag tatgatgaag aggcggggtt gtactataac cgtaaccggt actacgatcc
     5881 gctaaagggc aagtatatga ctcagggaaa gatcatcaga agttcacttt ttgtactaaa
     5941 taattcgcat tt
//
LOCUS       DAZPJR010000217         5838 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPJR010000217 DAZPJR010000000
VERSION     DAZPJR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.222"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(38..400)
                     /locus_tag="ACHVB7_003982"
     CDS             complement(38..400)
                     /locus_tag="ACHVB7_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1915836.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="ACHVB7_003983"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="ACHVB7_003983"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR1915837.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHVB7_003984"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHVB7_003984"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1915838.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHVB7_003985"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHVB7_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR1915839.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHVB7_003986"
     CDS             complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHVB7_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR1915840.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="ACHVB7_003987"
     CDS             5321..5683
                     /locus_tag="ACHVB7_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR1915841.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1469 a         1443 c         1518 g         1408 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgagat
     5821 tctgaaaatg cattttta
//
LOCUS       DAZPJR010000218         5836 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPJR010000218 DAZPJR010000000
VERSION     DAZPJR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.223"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHVB7_003988"
     CDS             complement(<1..242)
                     /locus_tag="ACHVB7_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1915842.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(604..3387)
                     /locus_tag="ACHVB7_003989"
     CDS             complement(604..3387)
                     /locus_tag="ACHVB7_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1915843.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3444..5816)
                     /locus_tag="ACHVB7_003990"
     CDS             complement(3444..5816)
                     /locus_tag="ACHVB7_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1915844.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1488 a         1320 c         1311 g         1717 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttacgagaat aatatttttt cattgaaaaa caatataata tgaaattctt gggtggtggt
      421 aaggtgtttt atgctgttat ttttatgcgc attctgtgcc tcctgaattt tcacgtaaaa
      481 atcagacctt aaaatatcac tattagtacc tggttattat tttgaacgca tttataaaat
      541 tattacataa aaatggcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      601 ttattatttt ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt
      661 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      721 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      781 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      841 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      901 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      961 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
     1021 gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
     1081 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
     1141 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
     1201 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
     1261 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
     1321 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
     1381 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1441 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1501 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1561 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1621 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1681 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1741 gcctttatta gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1801 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1861 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1921 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag
     1981 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     2041 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     2101 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     2161 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     2221 tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg
     2281 ctgatcaatg gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc
     2341 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     2401 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2461 gaacagctga actaacatgc tccattcagc ttgttcgaca aagctttgct catcgttcac
     2521 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt
     2581 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2641 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2701 gatatcgccg accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2761 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2821 taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2881 atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat
     2941 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     3001 tttctgcgta gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt
     3061 aacatcgcga ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     3121 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     3181 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg
     3241 ttgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt
     3301 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     3361 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3421 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3481 ccactggcgt aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt
     3541 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3601 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3661 tctgaatatt cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc
     3721 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3781 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag
     3841 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3901 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct
     3961 acttggcgga tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg
     4021 gttttgccat cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     4081 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc
     4141 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     4201 tgataacgcg tcagagtttt attacctgat accgattccg tccagctatt gcggatatca
     4261 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt
     4321 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     4381 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4441 atgtgctccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4501 taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc agactggtta
     4561 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4621 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4681 gcctgggaaa tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta
     4741 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4801 caaccaacgg tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga
     4861 ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4921 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4981 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg
     5041 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     5101 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     5161 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     5221 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     5281 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     5341 aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     5401 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5461 gggttcaggt tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac
     5521 gcaccgtgaa tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc
     5581 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5641 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5701 acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg
     5761 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5821 ttatatctcc gtacag
//
LOCUS       DAZPJR010000219         5620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPJR010000219 DAZPJR010000000
VERSION     DAZPJR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.224"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHVB7_003991"
     CDS             complement(<1..513)
                     /locus_tag="ACHVB7_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1915845.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHVB7_003992"
     CDS             complement(759..1211)
                     /locus_tag="ACHVB7_003992"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1915846.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHVB7_003993"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHVB7_003993"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR1915847.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHVB7_003994"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHVB7_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR1915848.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHVB7_003995"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHVB7_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHVB7_003996"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHVB7_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR1915849.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="ACHVB7_003997"
     CDS             complement(5494..>5620)
                     /locus_tag="ACHVB7_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1915850.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAZPJR010000220         5614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPJR010000220 DAZPJR010000000
VERSION     DAZPJR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.225"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHVB7_003998"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHVB7_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR1915851.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHVB7_003999"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHVB7_003999"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR1915852.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHVB7_004000"
     CDS             2475..4124
                     /locus_tag="ACHVB7_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR1915853.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="ACHVB7_004001"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="ACHVB7_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1256 a         1270 c         1456 g         1632 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAZPJR010000221         5554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPJR010000221 DAZPJR010000000
VERSION     DAZPJR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.226"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..115)
                     /locus_tag="ACHVB7_004002"
     CDS             complement(<1..115)
                     /locus_tag="ACHVB7_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1915854.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="ACHVB7_004003"
     CDS             complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="ACHVB7_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR1915855.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2333..2968)
                     /gene="nth"
                     /locus_tag="ACHVB7_004004"
     CDS             complement(2333..2968)
                     /gene="nth"
                     /locus_tag="ACHVB7_004004"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR1915856.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="ACHVB7_004005"
     CDS             complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="ACHVB7_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR1915857.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="ACHVB7_004006"
     CDS             complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="ACHVB7_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR1915858.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="ACHVB7_004007"
     CDS             complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="ACHVB7_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR1915859.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5350..>5554)
                     /locus_tag="ACHVB7_004008"
     CDS             complement(5350..>5554)
                     /locus_tag="ACHVB7_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915860.1"
                     /translation="AEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKA
                     AVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         1455 a         1411 c         1368 g         1320 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 acgctgcgga acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc
      541 gatgctctgc aggccatagc ttgcgaccag ccagcttaca gacacgatac cagcgtcaga
      601 cgcgaatttc gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat
      661 tgcaaacttg gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat
      721 cggactaccg atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc
      781 cagacccaga atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat
      841 ctggctgtac agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat
      901 tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc
      961 gaaggcaaca acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc
     1021 gatagcgatc agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg
     1081 ctcgaagtct ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac
     1141 gatagtgatc agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc
     1201 ggccagccac ggcgtagcaa tcatagagaa gaaagagccg atgttgacgg acatgtagta
     1261 catggtgaat gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga
     1321 agatgggtta gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac
     1381 gataccggcg tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc
     1441 accgagcata attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc
     1501 gaccagacca taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga
     1561 catacccagt tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa
     1621 acgttcccat aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt
     1681 caaactgacg ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac
     1741 atcccatatt aacgggggtg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt
     1801 atagtgggag gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt
     1861 gtaataatct gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc
     1921 agcgttaaat ttttcaaaaa acgtaatggc agattttttc actgcactga gaggggggtg
     1981 atggcttaac ggcgatatgt tctgctattt agcgttttta ctagtgagat agtccagttt
     2041 ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt gttgcgtgtg taaatttaaa
     2101 ttattttaac ctgattcttg caatgttttt acattgcgac agcatcgtga tcttgatcac
     2161 gaatgaatag aaaatttcgc tattaaaaaa acaattaacc tgcatttttg gttatccgca
     2221 aacggattac ctgatgataa cgctaagcaa tggcatcatt cacgttaaaa agatgtttta
     2281 aaagaggata aagaaaggtt atcaatgggg taatcggtgt tacccctttt cttcagatgt
     2341 caactttctc tttgtattca caaagatctt caataataca agagccacag cggggcttgc
     2401 gggcaatgca ggtataacgc ccgtgcagga tcaaccaatg gtggcagtcg actttaaact
     2461 ctgccggaac cactttcagt agcttttctt ctacctgttc gacgtttttc cccggcgcaa
     2521 attgagtacg attacaaacg cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga
     2581 atgcagtgtt taatacgacg ttggctgttt tacgacctac gccgggcagg gcttcaagcg
     2641 cagcacgatc ttccggaacc tcgccattat gctgctccag taagatacgg caggttttga
     2701 tgatattttc tgctttgctg ttataaagcc caatcgtttt gatataggtt ttaacccctt
     2761 caacgcccag ttcaagcatc gctgcaggcg tattcgccac cgggtaaagt ttcgccgtcg
     2821 ccttattaac actgacatcg gtcgcctgag cggaaagcag tacggcaatc agcaattcaa
     2881 aaggcgaact gaaattaagc tcggtggtgg gatgaggatt gttctcacgc aggcgagtga
     2941 ggatctccag gcgttttgct ttgttcatca gacattccct gtttcaccgt ttggcaatgc
     3001 acgttcggca gctgcttcag tacggcgctt tttcattctt tcatcaatca ggtattttcc
     3061 tgccagcatc agtcccaggc caataaatgc acctggtggc agcatcgcca gcaggaaagg
     3121 ggagtcggtg tggaaaatct ccacgcgtaa tacttttgcc cagctaccta acagcgcatc
     3181 tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt agtgaaccca gcacgaacat
     3241 ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt gccgaaagcg ccggaccttt
     3301 tttggcggcg aaggcttcag cgcggcccac aacgatacag ttagtgacaa tcagcggaat
     3361 aaaaatccct aatgattgat acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc
     3421 gctgaccacc gaggcgatga tcatcacgta aatgggaatg cggatctcgg ctggcgtcca
     3481 gtgacgcagc gtcgaaatgg tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc
     3541 taaacccaga gcgttagtgg cagtggacgt gaccgccaac agaggacaaa ggccgagcaa
     3601 ctggaccagc gcagagttgt ttttccacaa cccctgaaca ataacgtctt taatttcgct
     3661 cacgctttat tctccacagg caggaagttg agaaagttgt gccggtaacg tctgagcgta
     3721 caatccggcg cgttttaccg cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa
     3781 ctggtcgaaa tcaccaccat ctttcttcac cgcccagtgc gcatcatccg caccactgat
     3841 ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc agttcgattt tatcgccaag
     3901 ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt accgtgccgt taaaatcggc
     3961 tcccaccagc agctgaatcg caccggaata gccatctggc gcggttgctt ccagaacggc
     4021 ggctaccggt ttgtcatcct gtttggcgat gtaaacccga tgctcacctt tacctaactc
     4081 tggcgcagtt accagatagc aactctgtgc cagcgtattg ttatagcgtt cggctggcag
     4141 cacctgatca aataatgcct tttgttgcag actggcctgt tcagcaatcg tcgttttggt
     4201 catctggttg atggccgcag ttaaccctgt tgaacccgct gcaaatagcg ctaacgtaat
     4261 gccgtgtttt cggatagttt tcagcatggt ttaccctttg cgatggccgt agacgcgcgg
     4321 acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc agcaggacgg caaaagccac
     4381 gccgtcagga tagccgccga aactgcggat caaccagact aataagcccg caagcgcgcc
     4441 gaaaataaga cgaccacgat tggtcgtaga agcggtaacc gggtcagtca aaataaagaa
     4501 tgcgccgagc atggtcgcac cagacagcag atgaatttgc ggtgctgcca gtgtttctgg
     4561 tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt actaagaagc tgaggggaat
     4621 atgccagcga atcgctttct gccatagcaa ccacaggccg ccagccagcc aggcgagatt
     4681 tacccattgc caaccagcgc ccgccagaat accgctgtag atcggatatt gcataatctg
     4741 ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta tccagcggtg tcgcctgact
     4801 aatgccatca atacctaagc gtagtgtgtt catatcacca ccactggcgg tatgcccgct
     4861 gaaaataacc tgtatggcgt cgataaaacc agggatgttg accgcaattt catgtggcgg
     4921 taaccagctg gtcatctgca ccgggaagga gatcagtaag accacataac caatcattgc
     4981 cggattaaac gggttttgcc ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa
     5041 caccgtaccc agcacgacca tccaccatgg cgcgaggggg ggaatactta ccgccagcaa
     5101 taagcctgtc agcaatgctg agttatcttt caacgttgcg gctaccgact gcttgcgtag
     5161 tttgagtacg agagcttcgg ctaacagagt actaaccgat gccaacagga tctgaacgag
     5221 agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct ggcacggctg cgagcaacac
     5281 caacagcata atgcgcgatg tctggcgctg gttatgggta taaggggagc tagctattct
     5341 gaataccatt tagtcctcgt ttacaacctt ctgctgggcg gcttttttgg cctgagcgcg
     5401 ggcaatagcc gcggcaactg ccgctttgcg cggatcaacc tgttcttctg gttctgcgtt
     5461 agtctgttgc tgttccagct tgcgcgcttt ggcacgggca atagcggctt cgacggcggc
     5521 tttgcgcgga tcaacctgtt cttctggttc cgca
//
LOCUS       DAZPJR010000222         5549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPJR010000222 DAZPJR010000000
VERSION     DAZPJR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.227"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            292..861
                     /locus_tag="ACHVB7_004009"
     CDS             292..861
                     /locus_tag="ACHVB7_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1915861.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            1589..1762
                     /locus_tag="ACHVB7_004010"
     CDS             1589..1762
                     /locus_tag="ACHVB7_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR1915862.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(2020..3348)
                     /gene="rutG"
                     /locus_tag="ACHVB7_004011"
     CDS             complement(2020..3348)
                     /gene="rutG"
                     /locus_tag="ACHVB7_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR1915863.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3369..3863)
                     /gene="rutF"
                     /locus_tag="ACHVB7_004012"
     CDS             complement(3369..3863)
                     /gene="rutF"
                     /locus_tag="ACHVB7_004012"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR1915864.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(3874..4464)
                     /locus_tag="ACHVB7_004013"
     CDS             complement(3874..4464)
                     /locus_tag="ACHVB7_004013"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR1915865.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4474..5274)
                     /gene="rutD"
                     /locus_tag="ACHVB7_004014"
     CDS             complement(4474..5274)
                     /gene="rutD"
                     /locus_tag="ACHVB7_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR1915866.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5282..>5549)
                     /gene="rutC"
                     /locus_tag="ACHVB7_004015"
     CDS             complement(5282..>5549)
                     /gene="rutC"
                     /locus_tag="ACHVB7_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1915867.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1465 a         1453 c         1385 g         1246 t
ORIGIN      
        1 cttctaaaaa tcttcgaaaa tatctgacag atgtctgtag ctgctaagta cccgtcacac
       61 cagctatgtg accccctttg caaaaaagcc aaatatcatt gactttctcg gtgaattgct
      121 ataaaggcat ctatctaggt ctttaactgt ataaatacac atgacaacaa acgcccgtta
      181 agtgtctata taaaaaggta gctgtagcta tagacaacaa ttgtgataat tgatttaatg
      241 gctatagaca agcaagacag acctttgttg actatataga gagataagaa catgcgtaca
      301 ttcctttatg ctcgtgtatc agttgatgac ctcaccacac agaaccaagt agaagctgtt
      361 aaacgtgctg gttatgaagt cagaccttca cgagtaattg aggaagttat tagtggggct
      421 acaccagcta tggacagacc acaatttgta aagctggtgg ataagctgga agaaggtgat
      481 tgtcttgtag tgaccaagat tgaccgctta ggaagggata acattgacgt acagaagacc
      541 gtcacaatgt tacttggtat gggtgttaag ctggtctgtc tagatttgcc tgtccaagac
      601 ctttcaagtg ctgaaggtaa acttattctt caactattca gtacctttgc agagtttgaa
      661 cttaaccgca tcagagagcg aaccagagag ggcttagaac gagctaaagc acaaggtaag
      721 gtgttgggtc gtcctgtagc taaaaacact acagaggccg ttttagagca taaggctaag
      781 ggattgtcac aaagtcaggt tgcaaagctt ctgaatctct ctgtgagaac ggttagccgt
      841 cactggacaa agacactgta aaaaaaactt agtagcatct tcatagcctc cttcatgggg
      901 gcttttttgt gtctgtaaca tatggtgacc aaacatctat atatcgttat atagccctgt
      961 atatagcact atatatagtg tctggagagg gacgactaac accctgcata gttccctgaa
     1021 aaggggcttc acagattctc tatatatttc aataagttag gataaaaagg gactataccg
     1081 atactgcata gaatctgtgt aggattgcat agtcataggg gttggaacaa gcccaactac
     1141 acctgataaa gcctgttatt aacaggtagt tgaatgtacg tgtttagcta tgtaaaagca
     1201 ccagaacttt agccatttct aaccactcct cgtgttatct ctattccgta gcgattcgct
     1261 accacttatt taaagataag acgtcctttt cagagtgcaa atttcacaac cacttatttg
     1321 atttataaca actttcacaa gcacgtaatt ttgtcgcaaa atgacacatt tttatctcat
     1381 cgcgtttttt taatcataag agcggcttat ggataattat tggagatgat atctattctc
     1441 gctaagaagc tgttgcagga tattaccaaa cgcgggtcta cccgcgtcag ttcactaagc
     1501 ttagtcccac gtagcgaaaa tatggcagcc gccatacgcc gcgttaattc tatgcaatat
     1561 gatgtctata cccagacgga ggtcagtaat ggcaaaccat cgaggcggtt ccggcaattt
     1621 tgcagaagac cgcgaaagag catcagaagc aggtaaaaaa ggtggacagc acagcggggg
     1681 taatttcaag aatgacccgc agcgcgcatc tgaagcaggt aaaaaaggtg gcaagagcag
     1741 tcacggcaaa agcgacaact agccgggcta atcaatgacg aatgcatttt tgtctgtagc
     1801 tcgtcaaaaa gccatcaccg ccggttaccc ggtggttgat actgatgaca aatgtaagct
     1861 tgcctgatgc gcgatgctta tcaggcctac cagaagattg caatatattg aatttgcact
     1921 gttttgtagg ccggataagg cgtttacgcc gcatccggca tgaacaatgc gtacgttgtc
     1981 aacaatctgc accgccgggt aaccccggcg gttttctgtt tatggctcct gatgaacaac
     2041 ttctggcggt ggaacgtcaa ccaaccttct acttaacaac gcattgagta aaatcgcgcc
     2101 aaaggtggct gtaccgatcc ctcccaacgt aaaaccgccc agcgtgagag caaaatcacc
     2161 cgcgcccagc actaaggtta ctgcgaccat aatcaaatta ccgttctggc ttaaatcgac
     2221 acggttttgt acccagatcc ttgcgcctgc gacggcaatc agcccgaaca caacaattga
     2281 tgcaccacca atcaccgcgg ccggaatggt atggatcagc gcgccaaatt tcggcgaaaa
     2341 gcccaacagc atggcgatga cggcagcagc aacaaacacc agcgtcgagt agacttttgt
     2401 cacggccatc acaccgatat tttcagcata ggtggtgacg ccgctaccgc cgacagagcc
     2461 ggaaagcatc gttgccagac catcaccgac gaatgctcgc cccatatacg ggtccatatt
     2521 gcgtccggtc atcccggcga ccgccttgag atgacctaag ttttccgcca ccagaatcac
     2581 cgccacgggc gcaatcagca tcattgcctg actattaaaa gcaggagtgg aaaaatgtgg
     2641 caaaccgaac caggcagcat ggctgacgag agtaaaatcg acggcttttc ccagccctaa
     2701 aacgttggtc atcacgccat acagcagaca ggcgacaatt aaacctacga gaatcaataa
     2761 ccgctggatc atgccacggg taaacaccgc caccagccca atacacagca ccgtcattac
     2821 cgccatccag ctatcaaagg ctgaagccga tacacttttc actgcgatag gcgctaagtt
     2881 caggccaatc gccatcacca ccgcacccgt caccaccggc ggcattagtc gttcaatcca
     2941 gcgtgtacca attttcatca ccaccaggcc aatgacggta taaaccagcc cacaggcgat
     3001 aatcccgccc agtgcgatgc tgatattcgg gttaatgccc tgaccgttaa agcccgtcgc
     3061 ggcaatcacc acgccgacaa aagccgcgct ggagccgaga taactgggga cgcgcccgcc
     3121 ggtaataaag aaaaacagta acgtgccgat ccccgacatt aaaatggaaa gattgggatc
     3181 cagccccatc agaatcggca ttaacaccgt cgcgccaaac atcgccaccg cgtgttgaac
     3241 gcccattact gccgtctgag caaacggcaa tcgttcatcc ggcgcgacca cgccgctctc
     3301 tgtagaggtc gattttaact gccagtgagg aaaaccgaac attgccatca gctgtctcct
     3361 taaggaggtt aacaagcagg gcgcatcagc gcgtgataac tgcgatcgaa ccacaccagc
     3421 ccgtagggtg tggtgtgacg atgaatcgct tcgatggcgc aaaacagaat gtcgtgggtg
     3481 ccgacgctca ccacctggct gatgcggcag tcaaacgaaa ccagagcctc ttccagttgc
     3541 gggcatccgg tcacacccgt ctgccagcgg gcggcggcaa agcggtgttc catgggcgtt
     3601 ttgccgccaa aaagatttga aagcggctcc tgcccggcgc tcagtgtatt tacacacagc
     3661 gttcgatttt cattgaatac cggccagacg gacgccccac gattcaggca caccagtaat
     3721 gtgggcggcg tgtcggtcac gctgcagacg gcgctggcgg tgaacccggc gcgcccggat
     3781 ggaccgtccg tggtgatgat attgaccgcc gcgcccatac aggacatcgc atcgcgaaag
     3841 gtttgttgat caactatgtt catagtttgc tccttacaac agcccgcagg cttcttcaaa
     3901 ggacagacgt ggcaggcgcg caaaaagttt gctgctatcg ccatagccga tattaatcag
     3961 cagattgctc ttcagcgtgc tgcccgcaaa aaaggcgtcg tcaacgtgtt gacggtcaaa
     4021 acccgacatc gggccggtat ccagtcccag cgcacggcaa gcgacgatca gataggccgc
     4081 ctgcatggag ctgttgcgaa acgctgtttc ttcggcaagt tgtgggctgg aggtaaacca
     4141 actgcgggca tcaccgtggg gaaacagtag tggtaaccgt tcataaaatt cactgtccca
     4201 ggcaacgata gcggtgacgg gcgcggtcag ggttttttgt agattgccgc tggaaagcgc
     4261 cgggtgcaga cgttcttttc cttctacagt gcggataaac acgatccgtg ccggagaaca
     4321 gttagctgat gtcggccccc attttatcgg ggcataaagc tcccgtaacg tctcatcgct
     4381 gacgggtgtc tcccgccagc cgttgtgagt gcgggcatcg gtgaacaggg tgctaagcgc
     4441 acctgggcta acggcttcgt tcatagcaat tccttacagg gcggcttcac ggtgatgtaa
     4501 caggctggca agcccgttga gtaacagagc attaaacgtt tcggggtcgg tcacgttgca
     4561 ggcgtgtccg ccatagcgca tcaccatttt ctggctatcg ggcagggcgg catgaagctc
     4621 actggaacat gctgatggca ccagcagatc atcactggcg cagatgattt gcaccgggca
     4681 gcggatgcga tccgcatggt gactaaagtc agcgcgtttg agggcgttaa gtcgacgcag
     4741 taaattattt ttgccctgaa aatgcgccag tgccagcgcg tcttctgcct caaggcgagg
     4801 tgcgcgggcc gccatccagt cggcgggata gaggaacaac ggctgcgctt ccacccatgc
     4861 ctgcgcgccg ccgctataca gtaatcgttc gcgaacctga aaacagcggc gcgtatgggc
     4921 gtttattcgt agccagccgt taacgctgat cagcacagtt accgacgcgg gataatccag
     4981 cgccagctgc attcccacca gcgcaccgag cgcatggccg accaccgcgt aatgctcaat
     5041 ccctgcggct accagcgcct gatgcagttc cgctgccatc tgggcgatac tgtaatcttc
     5101 tgccagcgtg tcgggattat tgccggtgcc gcgctggtcg taacagacta cctgatactc
     5161 ctgctccagc accgccagtt gcggtaacca gtaactgccg ctaccgccaa gacccgaaat
     5221 caacaccact acgggcgcat cagcataagg gggaggtgag agtgaaagtt tcatcgcggc
     5281 ctcacttggc gatatgcgca attgtggcga tttccaccag cgcgtcaggt tttaccagtc
     5341 cgcactgaat gcagaatcgc gccggtttat cacccggaaa aaactcggcg tagatttcgt
     5401 taatcgcggc gtaatttttc cagtcggtaa taaagatgct gttgaaggtc acatccgcca
     5461 tcgtgccacc cgccgtctcg atcaccttgc ggatagtttc cagaacgtgg cgggtttgcg
     5521 cctttgggtc atcggcaaac agcacgtta
//
LOCUS       DAZPJR010000223         5527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPJR010000223 DAZPJR010000000
VERSION     DAZPJR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.228"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHVB7_004016"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHVB7_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR1915868.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHVB7_004017"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHVB7_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR1915869.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHVB7_004018"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHVB7_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR1915870.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHVB7_004019"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHVB7_004019"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR1915871.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHVB7_004020"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHVB7_004020"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR1915872.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHVB7_004021"
     CDS             4447..4812
                     /locus_tag="ACHVB7_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR1915873.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHVB7_004022"
     CDS             4962..5480
                     /locus_tag="ACHVB7_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR1915874.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1520 a         1391 c         1240 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZPJR010000224         5526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPJR010000224 DAZPJR010000000
VERSION     DAZPJR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.229"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHVB7_004023"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHVB7_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR1915875.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHVB7_004024"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHVB7_004024"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR1915876.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHVB7_004025"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHVB7_004025"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR1915877.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHVB7_004026"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHVB7_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR1915878.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1361 a         1325 c         1503 g         1337 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccg
//
LOCUS       DAZPJR010000225         5296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPJR010000225 DAZPJR010000000
VERSION     DAZPJR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.230"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..130)
                     /locus_tag="ACHVB7_004027"
     CDS             complement(<1..130)
                     /locus_tag="ACHVB7_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1915879.1"
                     /translation="MDTRVAFMTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPS"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="ACHVB7_004028"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="ACHVB7_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHVB7_004029"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHVB7_004029"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR1915880.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHVB7_004030"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHVB7_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR1915881.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="ACHVB7_004031"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="ACHVB7_004031"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR1915882.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5296)
                     /locus_tag="ACHVB7_004032"
                     /pseudo
     CDS             complement(4172..5296)
                     /locus_tag="ACHVB7_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1384 a         1331 c         1261 g         1320 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagc
//
LOCUS       DAZPJR010000226         5281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPJR010000226 DAZPJR010000000
VERSION     DAZPJR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.231"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..471)
                     /locus_tag="ACHVB7_004033"
     CDS             complement(<1..471)
                     /locus_tag="ACHVB7_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1915883.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(471..1391)
                     /locus_tag="ACHVB7_004034"
     CDS             complement(471..1391)
                     /locus_tag="ACHVB7_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1915884.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1529..2428
                     /gene="pgrR"
                     /locus_tag="ACHVB7_004035"
     CDS             1529..2428
                     /gene="pgrR"
                     /locus_tag="ACHVB7_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1915885.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2765..4378
                     /gene="mppA"
                     /locus_tag="ACHVB7_004036"
     CDS             2765..4378
                     /gene="mppA"
                     /locus_tag="ACHVB7_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR1915886.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTARFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="ACHVB7_004037"
     CDS             complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="ACHVB7_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR1915887.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1430 a         1203 c         1225 g         1423 t
ORIGIN      
        1 gccaatcgga tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt
       61 aatactcact actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc
      121 acccccacac actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc
      181 ataatcgata acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg
      241 ctccccacca gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc
      301 agccaatgcc ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg
      361 gacgctgata atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt
      421 ggcagcaata tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt
      481 tcctcaaaga acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa
      541 agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga
      601 tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc
      661 ggctgagtca ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg
      721 ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac
      781 tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc
      841 ataacggcat attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg
      901 tatggcaggg catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca
      961 atattgaccg cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg
     1021 gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa
     1081 gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt
     1141 tctaactgac gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg
     1201 acaaatccct tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc
     1261 ccacctgggt gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg
     1321 aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta
     1381 ttattcatca ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac
     1441 tatagatatt gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa
     1501 tgattaatag attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc
     1561 gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc
     1621 gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct
     1681 gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct
     1741 tggccctatg ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg
     1801 cccatccggg acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc
     1861 agcaatgcgc acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta
     1921 tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat
     1981 ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc
     2041 accggattat ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca
     2101 ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg
     2161 tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat
     2221 cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg
     2281 ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg
     2341 ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga
     2401 taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc
     2461 tcttaacgta ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg
     2521 cgacagatgc tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg
     2581 ttcggtgttc tatgtgcttt accgcatttt gtcgtctatt atttacacta aatctgattt
     2641 aatatattga tacataaaac attttatgct ccctgtgtca cttttttgat ggaagttgtt
     2701 tgcatttctt taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa
     2761 acttatgaag cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct
     2821 ttcgtatgct gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg
     2881 ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat
     2941 tcaggtcatt cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt
     3001 ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg
     3061 cgataacgca aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg
     3121 gcagcgtctg gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg
     3181 aatcaacaac gcacaggcga ttattgatgg taaagctacg ccagaccagc ttggcgtcac
     3241 cgcagttgat gcccatactt tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa
     3301 tttaaccgct aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga
     3361 gtggacgaaa cccggaaatc tgatcggcaa tggcgcttat gttcttaaag atcgcgtagt
     3421 caatgaaaaa ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact
     3481 gcaaaaagtg accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc
     3541 gggggatatt gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga
     3601 tattccgggg caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac
     3661 gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca ttaagtatga cgatagatcg
     3721 ccgcctgatg accgaaaaag tattagggac gggcgaaaag ccagcgtggc attttacacc
     3781 agatgttacc gccagattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga
     3841 actgaatgcg caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct
     3901 gaagctgacg cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc
     3961 atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac
     4021 ctatatcgat agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga
     4081 ttataatgaa ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc
     4141 acgctttaac aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt
     4201 taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc ctcatggagc aagcaccgat
     4261 tgcaccaatt tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc
     4321 cattaataac ccggaagatg tggcgtacag ccggactatg tatattgtga agcattgatg
     4381 tgatgggaac tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg
     4441 ttccggcggt gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc
     4501 gtgtgactgc acaatatcga taatcttcaa ataaacgtct tgctgtgcag caagccattc
     4561 agcccatacc gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa
     4621 ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat
     4681 ctcacgtacg gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat
     4741 ggtcgtggta atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa
     4801 cagcgagttc ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca
     4861 gccaatttcc gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat
     4921 actgaaagga cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt
     4981 accggccata ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct
     5041 catgccgaaa tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat
     5101 gcgcgacata atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa
     5161 ctgattaatc agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat
     5221 aaaatcgata acgtctgggg tgataaattt gagtttatag ttttctataa cataattaat
     5281 a
//
LOCUS       DAZPJR010000227         5219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPJR010000227 DAZPJR010000000
VERSION     DAZPJR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.232"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..227
                     /locus_tag="ACHVB7_004038"
     CDS             <1..227
                     /locus_tag="ACHVB7_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915888.1"
                     /translation="KNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPA
                     IIHSALNPTHYYQPVNDETTVMEIIKAYSK"
     gene            complement(291..575)
                     /locus_tag="ACHVB7_004039"
     CDS             complement(291..575)
                     /locus_tag="ACHVB7_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="HFR1915889.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(593..790)
                     /locus_tag="ACHVB7_004040"
     CDS             complement(593..790)
                     /locus_tag="ACHVB7_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915890.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESEWEEHSH"
     gene            1002..1298
                     /locus_tag="ACHVB7_004041"
     CDS             1002..1298
                     /locus_tag="ACHVB7_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915891.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
     gene            complement(1843..2076)
                     /locus_tag="ACHVB7_004042"
     CDS             complement(1843..2076)
                     /locus_tag="ACHVB7_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR1915892.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2083..2613)
                     /locus_tag="ACHVB7_004043"
     CDS             complement(2083..2613)
                     /locus_tag="ACHVB7_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR1915893.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(2651..3349)
                     /locus_tag="ACHVB7_004044"
                     /pseudo
     CDS             complement(2651..3349)
                     /locus_tag="ACHVB7_004044"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3291..3614)
                     /locus_tag="ACHVB7_004045"
     CDS             complement(3291..3614)
                     /locus_tag="ACHVB7_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR1915894.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            3679..3870
                     /locus_tag="ACHVB7_004046"
     CDS             3679..3870
                     /locus_tag="ACHVB7_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1915895.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            4538..4651
                     /locus_tag="ACHVB7_004047"
     CDS             4538..4651
                     /locus_tag="ACHVB7_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1915896.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
BASE COUNT         1373 a         1427 c         1116 g         1303 t
ORIGIN      
        1 aaaaaaacaa aggcgtcata atagaagtaa acacagatag tttatgtaag aaaaaactaa
       61 acgaaataac tagtaaaaat atagggaaac ccctgatttc atattttgat ggaaaagtaa
      121 tatcccaacc tgcaatcatt cattctgcac taaatccaac ccattattat caacctgtga
      181 acgatgaaac aacagtgatg gaaatcataa aagcatattc aaaataatat taatagatat
      241 aatcctgctt aaggtatagc ttaggcagga ttatatctat tcagctctta ttataaggta
      301 gtgtatacat gaataatctg ccctgtccca ctgacacttt taaatttaat tttatcaaca
      361 ccagcgccct tgcaagagat ctgataagat gctggaccaa taacgttaac tgttcttggc
      421 ccattagggg cataccagat aaaagcaata aatggaccgg gtacattaga caatgtatca
      481 aaatagacag tgtacgcgat atcaccagta tgattttcaa gttcaaccca aggaccaaaa
      541 aagaaaggag gagcccaaac attttgagaa atcatataat ctcctgatta gttcaatgcg
      601 aatgttcttc ccattcactc tcgcaagaaa catctataag ctcaacatct agcccactaa
      661 cagaatcaga gaaatactta taaacatcat cccttgttcc cataaggtct gccgaacatg
      721 atagttcaca accattctta ttcctaacta caaataagaa acggtgaagc aatacttttt
      781 cttctttcat tttatccctc cctttattta cacagaatat actgtatata taaacactag
      841 ttttatatac agtataaatt acgccgcatt atttttcctg tcaatgatat gaattttcat
      901 ttttgtgatc tgaatcaccc ttataaaaat caggaaggga agattcgcag cagaaaaaca
      961 gcaccggata acatcaggag aaaaaacaga gaggagataa cgtgagcaaa acaaaatctg
     1021 gtcgccaccg actgagcaaa acagacaaac gcctgctggc tgcacttatc gctgccggat
     1081 acgaagagcg gacagcccgt gacctcatcc agaaacacgt ttacacactg acacaggccg
     1141 acctgcgcca tctggtcagt gaaatcagta acggtgtggg acagtcacag gcctacgatg
     1201 cgatttacca ggcaagacgc attcgtctcg cccgtaaatt cctgaacgga aaaaaaccgg
     1261 aagaagggct gaacgcctgg aagggtgatt taccataatc acttattgta cgcaccgctg
     1321 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1381 aactgccgcg atcgtacccg gtaaatttta accacatgca tagctatgca gctatgtgaa
     1441 tcatgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1501 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1561 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1621 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     1681 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     1741 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     1801 gggataacgc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     1861 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     1921 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     1981 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2041 taatgcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct gtaccagcct
     2101 tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2161 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2221 cagcttctcc agattctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2281 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2341 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2401 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2461 agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt tttcgagctt
     2521 cctgaactcg tcgttcagta ttgtctctgt agattcggct ctctctttca gctttttctc
     2581 gaactccgcg cccgtctgta aaagattgct cataaaatgc tcctttcagc ctgatattct
     2641 tcccaccgtt cgggtctgca atgctgatac tgcttcgcgt caccctgacc acttcaagcc
     2701 ctgcctctgt gagcgcctga atcacatcct gacggccttt tatctccccg acatgataaa
     2761 gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc ggcaggttat
     2821 cagagagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc ccgtaatggt
     2881 gattcaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct
     2941 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3001 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3061 gtacacgctc aaagctcgcc atcacctttt cacgtcctcc cggtggcagc tccttctccg
     3121 cgaacgacag cacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3181 gtacttcttc cggattaccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca
     3241 gcaggtaatc aaccggacca ctgccaccac cttttccccg ggcatgaaac ttaactatca
     3301 tcccgcgtcc cctgttccct gacggccaga cgcagccggc gcagctcatc cccgatggcc
     3361 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc
     3421 ctcgctgtct gattcaggtt attcccgatg gcggccagct gacgcagtaa cggcggtgcc
     3481 agtgtcggca gcctcccgga acgggcaacc ggctcaccca ggcagacccg ccgcatccat
     3541 accgccagct gtttaccctc acagcgttca agtaaccggg catgttcatc atcagtaacc
     3601 cgtattgtga gcatcctctc gcgtttcatc ggtatcatta tccccatgaa cagaaatccc
     3661 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     3721 ataagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     3781 gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     3841 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3901 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcgcgtcagc aggttttagc
     3961 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcggc atcagtgcgg attgtatgaa aagtacgcca tgccgggtgt gaaatgccgc
     4081 acagatgcgt aaggagaaaa tgcacgtcca ggcgcttttc cgcttcctcg ctcactgact
     4141 cgctacgctc ggtcgttcga ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     4201 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     4261 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     4381 tccccccgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 aacgccactg gcagcagcca ttggtaacta aaaagtggat ttagatacgc agaactcttg
     4681 aagttgaagc cttatagcgg ctacactgaa aggacatctt ttggtatctg tgctccactt
     4741 aagccagcta cccggttagg taatctgcca ggattagcta accttcgaaa aaccacctgc
     4801 cagggtggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttttct ctaaactgca atcccatcag ttcaggagag aaagatagta
     4921 taattgccct ctcttatatg gaaccaccat tcaatcacat catgggaata atataagtga
     4981 aaacgttcgc attgtttatt tttacgtgta tatgtattta tagattggaa ttttaacaaa
     5041 ataaaatgga gttaatatgt tcaaatcatc agtaaagtca ataacatcga gtactatact
     5101 gaccttactt ataggatgtt caagtactga aacaaacaaa aacattgttt acaaagttgg
     5161 tggtgacagc atcattctga ataaatattg catacttaaa atgtaccctt cccaatcag
//
LOCUS       DAZPJR010000228         5196 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPJR010000228 DAZPJR010000000
VERSION     DAZPJR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.233"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..299
                     /locus_tag="ACHVB7_004048"
     CDS             <1..299
                     /locus_tag="ACHVB7_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR1915897.1"
                     /translation="QQSQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLP
                     PGISESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            299..>5196
                     /gene="traI"
                     /locus_tag="ACHVB7_004049"
     CDS             299..>5196
                     /gene="traI"
                     /locus_tag="ACHVB7_004049"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR1915898.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSV"
BASE COUNT         1154 a         1338 c         1763 g          941 t
ORIGIN      
        1 cacaacagtc acaacagcca gtgtcttctg tcatcaatga taagaagtca gatgcaggtg
       61 tgagtgttcc ggcagggggg atcgagcagg agctgaaaat gaaaccggaa gaagagatgg
      121 aacagcaact gccacccggg atcagtgaat ccggtgaagt ggtggatatg gccgcttatg
      181 aggcatggca acaggaaaat catccggaca tccagcagca tatgcagcgt cgtgaagagg
      241 tgaacattaa tgtgcaccgg gagcgcgggg aggatgttga gccgggagat gatttctgat
      301 gttgagtttc agcgttgtta aatcggcagg cagtgccgga aactattaca ccgacaagga
      361 taattactat gtgctgggca gcatgggaga acgctgggcc ggtcaggggg ctgaacagct
      421 tgggctgcag ggcagcgtcg ataaggatgt ttttacccgt cttctggagg gcaggctgcc
      481 ggacggggcg gatttaagcc gcatgcagga tggcagtaat aagcatcgtc ccggctacga
      541 cctgaccttc tccgccccca aaagtgtctc catgatggcc atgctcgggg gggataaacg
      601 tcttattgat gcacataacc aggccgtgga ttttgctgtc cgtcaggtgg aggcgctggc
      661 ttccacacgg gtgatgacgg acggacagtc agaaacggtg ctgaccggta atctggtgat
      721 ggcactgttt aaccatgaca ccagtcgcga tcaggaccca cagttacaca cgcatgtggt
      781 ggtggctaat gtcacgcagc ataacggcga gtggaagaca ctgagcagtg acaaagtggg
      841 gaaaaccgga ttttccgaga acgtgctcgc gaaccggatt gcgttcggga aaatatatca
      901 gagtgagtta cgtcagcgcg ttgaggcgct gggttatgaa accgaagtgg tcggtaagca
      961 cggtatgtgg gagatgccgg gcgtaccggt ggaggccttt tccagtcgtt cacaggctat
     1021 cagggaggcc gtcggtgaag acgcctcgct gaaatcccgg gatgtggcgg cactggatac
     1081 gcgaaaatcc aaacagcacg tcgatccgga aatcagaatg gctgaatgga tgcagacgct
     1141 gaaggaaacc gggttcgaca tccgggcata tcgtgacgcg gcggatcagc gtgcggagat
     1201 ccgcacgcag gcgcccgggc ctgcttcaca ggacgggccg gatgtgcagc aggcggtgac
     1261 acaggcgatt gccggattaa gtgaacgaaa agtgcagttc acgtatacgg acgtgctggc
     1321 caggacggtc ggcatactgc cgccggaaaa tggtgtgatt gaacgggcac gcgccggtat
     1381 cgatgaggcc atcagccgtg agcagcttat ccccctcgac cgtgagaagg ggctgttcac
     1441 atcaggaatt catgtgctcg atgagctgtc cgtccgggca ctcagtcgtg acatcatgaa
     1501 acagaaccgg gtgaccgtac atccggagaa aagtgtcccc cggacggctg gttacagcga
     1561 tgcggtcagt gttctggcac aggaccgtcc gtcgctggcc attgtgtccg ggcagggcgg
     1621 tgcagccggg cagcgtgagc gggtggctga actggtcatg atggcccggg aacaggggcg
     1681 ggaggtgcag attatcgctg ctgaccgtcg ctcgcagatg aacctgaagc aggatgaacg
     1741 gctgtccggt gaactgataa ccggacgtcg tcagctgctg gaaggcatgg ccttcacgcc
     1801 gggcagtacc gttatcgtcg accagggtga aaaactctcc ctgaaagaga cgttaaccct
     1861 gctggatggt gccgcacgtc ataacgtaca ggtcctgata accgacagcg ggcagcgaac
     1921 cggtacaggc agtgcgctga tggccatgaa ggatgccggg gtgaacacat atcgctggca
     1981 gggcggagaa cagcgaccgg ccaccatcat cagtgaaccg gaccgtaatg tccgctatgc
     2041 ccggctggcc ggagattttg cggccagcgt gaaagccgga gaagagagcg tggcacaggt
     2101 cagcggggta cgggaacagg ccatactgac acaggccatt cgcagtgagc tgaaaacgca
     2161 gggcgtgctc ggacaccctg aggtgaccat gactgcactg tcaccggtct ggctggacag
     2221 ccggagccgt tatctgcggg atatgtaccg accggggatg gtgatggagc agtggaaccc
     2281 ggagacacgc agtcatgacc gctatgttat cgaccgggtg acggcgcaga gtcacagcct
     2341 gaccctgcgg gatgcgcagg gcgaaacgca ggtggtgcgt atttcctccc tggacagcag
     2401 ctggtcgctg ttccggccgg aaaaaatgcc ggtggcagac ggcgagcgac tgagggtgac
     2461 agggaaaatt cccggactcc gcgtctccgg cggtgaccgc ctgcaggtgg catccgtcag
     2521 tgaagatgcg atgacggttg ttgtgccggg gcgggctgaa ccggccaccc tgcctgtggc
     2581 tgattcaccg ttcacggcac tgaagctgga gaacggctgg gtggaaacgc ccgggcattc
     2641 cgtcagtgac agtgcgacgg tgtttgcctc cgtcacacag atggcaatgg acaacgccac
     2701 cctgaacggt ctggcccgca gcggtcgtga tgtccggctg tattcctcac tggatgaaac
     2761 ccgtactgcg gaaaaactcg cccgccatcc gtcctttacg gtggtttctg agcagataaa
     2821 ggcgcgtgcc ggtgagacat tgctggaaac cgctatcagt ctgcagaaag ccgggcttca
     2881 cacgccggca cagcaggcca ttcatctggc ccttcctgtg ctggaaagta aaaacctggc
     2941 cttcagcatg gtggacctgc tgacagaggc gaagtcgttt gctgcagaag gaaccggttt
     3001 tactgaactg ggaggggaaa tcaatgcgca gataaaacgc ggtgacttac tgtatgtgga
     3061 tgtggcaaaa ggctatggca caggcctgct ggtttcccgt gcgtcgtatg aggcagaaaa
     3121 gagcattctt cgccatattc tcgaaggcaa ggaggcggtc acgccgctga tggagagagt
     3181 acccggcgaa ctcatggaga cgttaacgtc gggacagcgt gccgccaccc gtatgatact
     3241 ggaaacgtcc gaccgtttca cggtggtaca ggggtatgcc ggtgtgggta agaccactca
     3301 gttccgggcg gtgatgtcag ccgtgaacat gctgccggag agtgagcgtc cccgggttgt
     3361 ggggctgggg cccacgcacc gtgcggtcgg tgagatgcgc agcgccggcg tggatgcaca
     3421 gacactggcg tcctttctgc atgacacgca gctgcagcag cgcagcggag aaacgccgga
     3481 tttcagcaac acgctgtttc tgctcgatga gagctcaatg gtgggcaata ccgacatggc
     3541 acgggcatac gccctgattg cggccggtgg cggtcgtgct gtggccagcg gtgacacgga
     3601 ccagctgcag gccatcgcgc ccggtcagcc tttccgtctc cagcagacgc gcagtgctgc
     3661 cgatgtggcc atcatgaagg agattgtgcg tcagacgccg gaactgcggg aggcggtata
     3721 cagcctgatt aaccgggatg tggaaagggc actgtccggg cttgagagtg tgaaaccgtc
     3781 tcaggtgcca cgtctggagg gcgcatgggc accggagcac tccgtgacgg agttcagtca
     3841 cagccaggaa gcgaaactgg cagaagcgca gcagaaggcg atgctgaaag gcgaggcttt
     3901 cccggatatc cccatgacac tgtatgaggc cattgtccgc gactataccg gcaggacacc
     3961 ggaagcacgg gagcagacgc tgattgtcac gcacctgaat gaggaccggc gcgtactgaa
     4021 cagcatgatt catgatgcac gggaaaaggc cggtgagctg ggcaaagagc aggtcatggt
     4081 gcctgtcctg aacacagcga atatacgtga cggagaactg cgtcgtctct ccacctggga
     4141 gaaaaacccg gatgcccttg ccctggtgga tagtgtgtat caccggattg ccggtatcag
     4201 caaggatgac gggctgataa ccctgcagga tgcggaaggt aacacgcggc tgatttcgcc
     4261 ccgggaggcg gtggctgaag gtgtcacact gtacaccccg gacaaaatcc gggtggggac
     4321 cggcgaccgg atgcgcttca cgaagagtga ccgggagcgc ggttatgtgg ccaacagcgt
     4381 ctggacggtg acagcggttt ccggtgacag tgtcacgctg tcggacggac agcagacccg
     4441 ggtgattcgc cccggtcagg agcgggcaga gcaacatatt gacctggcct atgccatcac
     4501 tgcccacggt gcgcaggggg cgagtgaaac ctttgccatc gcgcttgaag gcacggaagg
     4561 taaccggaaa cagatggccg gctttgagtc agcctacgtg gccctgtcgc gtatgaagca
     4621 gcatgtgcag gtgtacaccg ataaccgtca gggctggacg gatgccatta acaatgccgt
     4681 acagaaagga actgcccacg atgtgctgga gccgaaaccg gaccgggagg tcatgaatgc
     4741 gcagcggctg ttcagtacgg cacgggaact gcgggacgtg gcggcaggcc gtgccgttct
     4801 ccgtcaggcg gggctggccg ggggagacag tccggcacgg tttattgcac cggggcgcaa
     4861 atatccgcag ccgtatgtgg cactgcctgc ttttgaccgt aacggcaagt ccgccggtat
     4921 ctggctgaac ccgctgacca cggatgacgg aaacgggctg cggggattca gcggtgaagg
     4981 acgggtgaaa ggcagcgggg atgcgcagtt tgtggccctg cagggcagcc gtaacggcga
     5041 gagcctgctg gctgataata tgcaggatgg tgtccggatt gcccgtgata accccgacag
     5101 cggtgtggtg gtgagaatcg ccggtgaagg tcgtccgtgg aatcccggtg ccatcaccgg
     5161 tggtcgcgtg tggggggata tcccggacaa cagcgt
//
LOCUS       DAZPJR010000229         5114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPJR010000229 DAZPJR010000000
VERSION     DAZPJR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.234"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..379
                     /locus_tag="ACHVB7_004050"
     CDS             <1..379
                     /locus_tag="ACHVB7_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915899.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="ACHVB7_004051"
     CDS             complement(575..2125)
                     /locus_tag="ACHVB7_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR1915900.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="ACHVB7_004052"
     CDS             2297..2743
                     /locus_tag="ACHVB7_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR1915901.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="ACHVB7_004053"
     CDS             complement(2937..3872)
                     /locus_tag="ACHVB7_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1915902.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="ACHVB7_004054"
     CDS             4384..>5114
                     /locus_tag="ACHVB7_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915903.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAZPJR010000230         5083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPJR010000230 DAZPJR010000000
VERSION     DAZPJR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.235"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHVB7_004055"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHVB7_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(796..1248)
                     /locus_tag="ACHVB7_004056"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHVB7_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHVB7_004057"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHVB7_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHVB7_004058"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHVB7_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR1915904.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHVB7_004059"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHVB7_004059"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHVB7_004060"
     CDS             complement(2927..3859)
                     /locus_tag="ACHVB7_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR1915905.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHVB7_004061"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHVB7_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR1915906.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1247 a         1176 c         1184 g         1476 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 tatcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt cgtcactgac
     5041 tatttccgat attcaggcgt tgaaaaatca aaaaactgga aaa
//
LOCUS       DAZPJR010000231         5044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPJR010000231 DAZPJR010000000
VERSION     DAZPJR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.236"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="ACHVB7_004062"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="ACHVB7_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1771
                     /locus_tag="ACHVB7_004063"
                     /pseudo
     CDS             1595..1771
                     /locus_tag="ACHVB7_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1779..1868)
                     /locus_tag="ACHVB7_004064"
                     /pseudo
     CDS             complement(1779..1868)
                     /locus_tag="ACHVB7_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHVB7_004065"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHVB7_004065"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR1915907.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="ACHVB7_004066"
     CDS             complement(3750..4700)
                     /locus_tag="ACHVB7_004066"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1915908.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="ACHVB7_004067"
     CDS             complement(4839..>5044)
                     /locus_tag="ACHVB7_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR1915909.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1230 g         1210 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAZPJR010000232         4882 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPJR010000232 DAZPJR010000000
VERSION     DAZPJR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.238"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(358..1401)
                     /locus_tag="ACHVB7_004068"
     CDS             complement(358..1401)
                     /locus_tag="ACHVB7_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1915910.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1813..3025)
                     /locus_tag="ACHVB7_004069"
                     /pseudo
     CDS             complement(1813..3025)
                     /locus_tag="ACHVB7_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3297..3391)
                     /locus_tag="ACHVB7_004070"
     tRNA            complement(3297..3391)
                     /locus_tag="ACHVB7_004070"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3355..3357),aa:Sec,seq:tca)
     gene            3684..>4882
                     /locus_tag="ACHVB7_004071"
     CDS             3684..>4882
                     /locus_tag="ACHVB7_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR1915911.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1276 a         1069 c         1117 g         1420 t
ORIGIN      
        1 ccacgggaat atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa
       61 atataggggc aaatccacgg ggataccagt tcaaccgaaa acgccagagg aggggattac
      121 ccgctggcag ggtaaatctg tggtttttga tgccagctat aacgtgtttg acgataaagt
      181 attacgaaag aactttaatg agttcatgga gtaattcgtt aaagtaattt acaacgctat
      241 ccttttgcaa aggaaacttt cagaaaataa ccccagggta atcacctggg gaaggaacac
      301 agtgttatta ttggataata aattattact tgttttaaag ttacactaat gcaatatcta
      361 ctgatactgg atgcttgaat gtttcatccc tgctgcggaa aagggcaata tccagggcca
      421 gatatattca ccgttcatat ttctgcattt ctgtacaatt tcctcagaaa gtgtcttgtc
      481 aattcttttc cacaggtgct cctgctgtct taaaatactt tcgcatattt cgaaatcatc
      541 ttcctgaatc agcatttcgg cgcatttaat gatttctgat cgttcatgca gcacggagga
      601 atggttatag agccagttta caaacagcaa tgcttccgga tattgtcccc ataatggtga
      661 gtatgttgca tagtaatctt ctgccgtatc cagagagccg gagaagtcat gccacacagc
      721 aagtggaaat ggatgcagat ttccgcacag catttctgcg atgtaagtgt ttgtggcaaa
      781 gccatggcga aaagctgtgg cggccttaac atgttccccg ttcagaaaat gcaggagacc
      841 gagttcatac cagtaaggtg aaaattcatc agcatgctct ttcagtacac tgaaggctcg
      901 ctcatgatcg ccggtgcgta acagctctga acccaacaac cagcgagccc cctgattatc
      961 ttcaggattg tatgccagta ttttatcagt gagcataacc gcatcctgat ggcgctgtaa
     1021 gtgcacattt gcgagaattg ctgcataaag cgcgcgcaga taaggccggt tttcgggatg
     1081 catccagatt atttcaccac agaaactttc cgggatgata cgattacctg ccgccagtcc
     1141 tttaagagag gcgttcagtg ccttacgcgg ggcattctgt tccagaaatg catatgccag
     1201 atgagcgtgt atatcaataa agtccggctc ccgttgagca aggcgtcgga gtttgttgat
     1261 gtaactcgtc tcgctgattt catcagcgtc gtatgcatca agcacttctt caaactcccc
     1321 cagcaatggg ccaaaccagg tatccggaaa tacgaaataa cccttgttat cgtgcacttc
     1381 aaagcataat ctatcgttca tttctgagcc tgtaaaacat gtggtgatat agtctgagag
     1441 cataaaagtt gagattctgt gtatcaagtc ctgtcttggc accgaatgaa tattgcttac
     1501 gaagcccctt catgctttcc ttgagaacaa taattttatc tttatgtgaa tctcattttt
     1561 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
     1621 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
     1681 gtttttaatt atgtttttgt aataagtttg aagacgcaaa aaaaataata taataacaaa
     1741 aatcctccag ccttgtaggc cagaggataa aatacttaat tgaggaaaat atcagggaaa
     1801 agtatttctg acttaacttt cccggctgga gagtgaatca atgtaatcag cgtaccattg
     1861 catcatttca cggcggccat cgagatacag ggcatgatta taggtacccc tgatggaatt
     1921 tttatccaca tgagctaact gcatttcaat ccaggcggaa ttaaagcctt gttcatgcag
     1981 aattgtactc atggtgtgtc tgaaaccgtg ccctgtgagt cttcccttat atccaagtaa
     2041 ttcaattact tggttaatgc tttccttact aattggtttg cggggatcat ttctaccgat
     2101 aaagactagg ggatgatgtt ttgagattgg ttcaagctgc ttaaatagca taatgacctg
     2161 ctcagagagc ggcacaatat gaggtcgctt catcttcatt acttctgccg ggatctccca
     2221 caattttgtc tcaaaatcga tatcttccca gcgtgcaaaa cgcagttctt gtgttcgtac
     2281 gccggttaac ataatgatct gagtagctgt cttagtgatt atacttccgg tataacccgc
     2341 cagatcgttg aggaagtggg gaagttcatt agcagtaaga aagggaaaat gtactttttt
     2401 cggtgtagct aaagcactgg caagatcggg ggctgggtta tagtctgccc ggccagtaac
     2461 aattgcatag cggaacacct caccacagcg ctggcggacc ttgcgcattt tctccagagc
     2521 tccacgtttt tccattttac gcagtgcttc cagtaactcc attggtttga tttcggcaat
     2581 agggcgcttg ccaatgtagg gaaaaatgtc tttttcaaag gtatcaatga tttcatcacg
     2641 atagcgtaat gaccaccgat cagcttttga ctggtgccat tcgcgagcaa tagcctcgaa
     2701 cgtatttttc agactcattt gctgcgcgat tttttcttcc ttttttgcct cccctggatc
     2761 gcctccagca gcgaggagtt tcctggcttc actgcgtttc tcgcgagcat cggctagcgt
     2821 tacatcgggg tatacgccaa tagaaagcat cttttcttta ccagcaaagc ggtacttcat
     2881 gcgccagtat ttagaaccat tagtgttaat cagtagatac ataccgccgc catcagcaag
     2941 cttatagggt ttgtctttgg gttttgcggt tttcactttg atatcggtta atgccatgac
     3001 atcctccggc ttttgatgaa ttcatgttcc tgagggggat ttctgccagt aactaacctg
     3061 atacccccaa tacacccctt gtataacaaa gatattgtta aaagccgttg ttatgtatca
     3121 catttttcct attatggggg tgcttgtatt tcaataggaa ataacacccc ctaaagtacc
     3181 cccaaaagaa acttgatatg gggtgaagta ggttgacctc aggatacaaa taaatactga
     3241 tatacaaggg attagaagag atttattgac attgggagag ttcagaagag gtaaactggc
     3301 ggaagatcac aggagtcgaa cctgcccggg accgctggcg gccccaactg gatttgaagt
     3361 ccagccgcct caccggagac gacgatcttc cgcgcctcga ttgctacatg gaggcggggc
     3421 gcattatagc tacttccttg agtttctaca tcccccagat cagttttttc cgcctaatac
     3481 acgcagcgta ttatcacgaa agcattataa ctcatataaa acccatattt tacaatgata
     3541 tatgagcgga attaacgcta ccacgatcac ataaaacaag aatcataaat taataaccag
     3601 atatcggaat attcgctctc acagggatgg tttacaaaat gcgttatcgg tctcagcacc
     3661 cctatagcat caaggaaaag cagatgaaga gtgaagtgtt gtccgttaaa gagaaaattg
     3721 gttatggcat gggagacgcc gccagccaca ttattttcga taacgtaatg ttatatatga
     3781 tgttctttta taccgatatt tttggcattc ctgccggatt tgtcggaacc atgtttttgg
     3841 tcgctcgtgc actggatgcg atttccgatc cttgcatggg gttgttggcc gatcgaacgc
     3901 gctctcgctg gggtaaattt cgtccgtggg tactgtttgg cgcactgcca ttcgggatcg
     3961 tctgtgtact ggcctatagc acgccagatc tcagtatgaa cggcaaaatg atctatgcag
     4021 caattactta caccctactt accttacttt ataccgtcgt caatatccct tactgcgcat
     4081 tgggtggtgt aatcaccaat gacccgactc agcgtatctc gctgcaatcc tggcgttttg
     4141 tgctggcgac cgcgggaggc atgctttcta ctgttctgat gatgccactg gttaatttaa
     4201 ttggcggtga taataaacca ctcggtttcc agggcggtat cgcggtcctt tccgtggtgg
     4261 cattcatgat gctggcattt tgtttcttca ccactaaaga acgcgttgaa gcaccaccta
     4321 caacaacgtc tatgcgggaa gatttacgtg atatctggca aaacgaccag tggcggattg
     4381 tcggtttact aaccattttc aatatcctgg cggtgtgcgt acgcggtggg gcgatgatgt
     4441 attacgtcac atggattttg ggcacgccgg aagtgtttgt cgcttttctc accacttatt
     4501 gcgtgggtaa cctgattggt tccgcactgg caaaacctct gaccgactgg aaatgtaaag
     4561 tcactatctt ctggtggacg aacgccctgc tggcagtgat tagcctcgcg atgttctttg
     4621 ttcccatgca ggccagcatc actatgtttg tcttcatctt cgtgattggt gtgttgcatc
     4681 aactggtgac acctatccag tgggtaatga tgtccgatac cgtcgactac ggcgagtggt
     4741 gcaatggtaa acgcctgacc gggatcagtt ttgctggcac gctgtttgtg ctcaaactgg
     4801 ggttggcctt cggcggcgct cttatcggct ggatgctggc ttatggcgga tatgatgcgg
     4861 cagaaaaagc gcagaacagc gc
//
LOCUS       DAZPJR010000233         4838 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPJR010000233 DAZPJR010000000
VERSION     DAZPJR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.239"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(263..463)
                     /locus_tag="ACHVB7_004072"
     CDS             complement(263..463)
                     /locus_tag="ACHVB7_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915912.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="ACHVB7_004073"
     CDS             complement(463..735)
                     /locus_tag="ACHVB7_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1915913.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="ACHVB7_004074"
     CDS             complement(738..1016)
                     /locus_tag="ACHVB7_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915914.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="ACHVB7_004075"
     CDS             complement(1017..1574)
                     /locus_tag="ACHVB7_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915915.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            complement(3138..3689)
                     /locus_tag="ACHVB7_004076"
     CDS             complement(3138..3689)
                     /locus_tag="ACHVB7_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1915916.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            4488..4784
                     /locus_tag="ACHVB7_004077"
     CDS             4488..4784
                     /locus_tag="ACHVB7_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR1915917.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT         1364 a          993 c          921 g         1560 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcttg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttacaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccagaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa
     2221 ttagccatat atagagaact aaaagatttt ttagctattt tgtttaaaag catatcaaac
     2281 gatacatttt attgatttag gtcaaagaca aaagcattga caaaatggta agattgttca
     2341 catgacctta cttaacagac ttttaagaga ctttgaaaca gcctgtttaa caggtcacta
     2401 cctctggtct tatcgttccc ttctttctag gtggttatct gttaagagtt tttcagtttt
     2461 tattcttaag ataattgtta agatgatact ctcccttcgg gttatatcgt atctcttctc
     2521 ccgttaaata tcccttcaca gtcagactaa ataacacctg tgtagttctt ggtagaatct
     2581 ttcaaggtaa cttagctctg agtttatgaa gttgacctat gagatagact ctaccgaatc
     2641 tgttagtcaa ttgttgctta ggtgctgcta aggcttactt agtggaaaga gaggggatat
     2701 gtgaccatag gggaacccct cctgttaaaa gactacttag aagtctgagg ttttagtaaa
     2761 atctgtgaat atggcttctc agacgctctg taacgcattc taagcgattt taccccttta
     2821 ttttaaggtg gttagtacat ccctataggt tacccctaaa aatggggtta aaactcgcta
     2881 cagagctttc tatgagatta gcttctaatc gaatcaacta tgaggtttgg taggattgtg
     2941 caaaatctgt acagtaactt ttcagaatct gaaaattttt ataaaaaatg actagccctc
     3001 tttgaatctc atcttccccg aatcttcaaa aatatctgac agatgtctgt agctgctaag
     3061 tacccgtcac accagctatg tgaccccctt ggcaaaaaag ccaaatatca gctacatcca
     3121 tgtaactatt cagtaactta tttagcaagt ctgtaataag ttgctctaga gattcctaac
     3181 tcttcctgaa tctgcaacct cgtcttacct tgtgaaataa gcgtctgtac agcctctgaa
     3241 gaagctttta caggcctccc catatgtacc ccttcagctt tagcacgttc aataccagcc
     3301 tttctacgct ctgctatcaa gcttctttca agctgtgcca acgttgacat aatgcctaac
     3361 attgcttcac ccatagcgct tgataagtca aacccttctc taaggctgat aactgtcaca
     3421 cctttagcct gtagcgcttt aaccgttgat aacacatcaa ttgtgtcacg tccaagacgg
     3481 tctacagcta ccacaactac ggtgtcacct tctcgtgcat aagctaatag tctgctgaaa
     3541 ccgtttctta aagaagcttt gacagaacca ctgacagctt catcagtgaa ccatttagca
     3601 atgttgtacc ctgcctcttc aatttgcatt tgctggttag tgatggattg gtcatctgta
     3661 ctcaccctgc aatatccaag tatcgccata atgttcccct tagaagtctc aaaagttagt
     3721 ctcataagtt tagctgtctc ataatccaaa aacaaccttt tgagactact tgagggtgca
     3781 ttctaccgtt acgttgaaag tgtctcaaaa ggaagtcctt ttaagactgg ttatgaagga
     3841 gctatgaggt tgattagggg ttggtacaag cccaactaac cgaatcaact atgaagattt
     3901 taggagggta actgtaacca tcactttcta actttcacgt aggaaattgt taagtggttt
     3961 gatagttaca ccatgtgacc aagcatctat atatcgttat atagccctgt atatagcact
     4021 atatgtagtg tcgagagagg aacgactaac acgatacagt ttaccctgaa aagagacttc
     4081 acagattctt tatatatttc aataagttag ggtaaaaagg gactataccg agactgtata
     4141 gaatctgtgt agaattgcat agatacttgg aaatctacta ttaaattgca tagggactca
     4201 cctgatattg tcctgttttt aacaggtctc ctagaaaacc gaatagactt cattcaaggg
     4261 tcattgaagt cataatcatt tcagtagaaa aaccaagtct cgattttaaa tgcaaatagc
     4321 aatcatattc aataaggcaa gacgatcagg atcagtgaaa atgtcatttt tggcgtaatt
     4381 gaccttactt tatgcatggt cttaacaaag acatcatctt tccgttgcag atcttcgccc
     4441 tttcaaataa cgtactttac aactttcccg aacaaggagt tgtgcccgtg ttgtatgtta
     4501 tttacgctca agacaaagct gatagcctcg aaaaacgcct ttccgttcgt ccggcacatt
     4561 tagcacgttt acagttactg catgatgaag gtcgtttgtt gacagctggt ccaatgccag
     4621 cggtagacag taacgatcca ggggctgcgg gatttactgg ctcaaccgtt attgctgaat
     4681 ttgaatcgct ggaagcagct caggcctggg ccgatgctga cccgtacgta gcggcaggtg
     4741 tctatgaaca cgtttcagtg aaaccattta agaaagtgtt ctgaatagag attcaatatt
     4801 taagggcacg gtttttgcaa ccgtgttctt gtttggcc
//
LOCUS       DAZPJR010000234         4811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPJR010000234 DAZPJR010000000
VERSION     DAZPJR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.240"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            195..1169
                     /gene="hemB"
                     /locus_tag="ACHVB7_004078"
     CDS             195..1169
                     /gene="hemB"
                     /locus_tag="ACHVB7_004078"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR1915918.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1275..2126)
                     /gene="tauD"
                     /locus_tag="ACHVB7_004079"
     CDS             complement(1275..2126)
                     /gene="tauD"
                     /locus_tag="ACHVB7_004079"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR1915919.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2123..2950)
                     /gene="tauC"
                     /locus_tag="ACHVB7_004080"
     CDS             complement(2123..2950)
                     /gene="tauC"
                     /locus_tag="ACHVB7_004080"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR1915920.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2947..3714)
                     /gene="tauB"
                     /locus_tag="ACHVB7_004081"
     CDS             complement(2947..3714)
                     /gene="tauB"
                     /locus_tag="ACHVB7_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR1915921.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3727..4689)
                     /gene="tauA"
                     /locus_tag="ACHVB7_004082"
     CDS             complement(3727..4689)
                     /gene="tauA"
                     /locus_tag="ACHVB7_004082"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1915922.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1113 a         1391 c         1231 g         1076 t
ORIGIN      
        1 aataataacc aagagctata aataaagatt aaaaatattt tcagtgaata tgaaattatt
       61 gctatttcta ttacaaagat gtcctggtct ctctttcatt ctcttcttgt caacatcacg
      121 acaactttcg taaaacactc ctgccccgct tcaggtatac tgtgcacctt aattccacaa
      181 acatcaggca gaccatgaca gacttaatcc aacgccctcg tcgcctacgc aaatctcctg
      241 cgctgcgcgc tatgtttgaa gagacaacac ttagccttaa cgacctggtg ttgccgatct
      301 ttgttgaaga agaaattgac gactacaaag ccgttgaagc catgccaggc gtgatgcgca
      361 ttccagagaa acatctggca cgcgaaattg aacgcatcgc caacgccggt atccgttccg
      421 taatgacttt tggcatctct caccataccg atgaaaccgg cagcgacgcc tggcgggaag
      481 atggactggt ggcgcgtatg tcgcgcatct gcaagcagac tgtgccagaa atgatcgtca
      541 tgtcagacac ttgtttctgt gaatacactt ctcacggtca ctgcggtgtg ctgtgcgagc
      601 atggcgtcga caacgacgcg actctggaaa atttaggcaa gcaagccgtg gttgcagctg
      661 ctgcaggcgc agacttcatc gccccttccg ccgcgatgga cggccaggta caggcgattc
      721 gtcaggcgct ggacgctgca ggctttaaag atacggcgat tatgtcgtat tcgaccaagt
      781 tcgcctcttc cttttatggc ccgttccgtg aagctgccgg aagcgcatta aaaggcgacc
      841 gcaaaagcta tcagatgaac ccaatgaacc gtcgcgaggc gattcgtgaa tcactgctgg
      901 atgaagccca gggcgcagac tgcctgatgg ttaaacctgc tggagcgtac ctcgacatcg
      961 tgcgtgagct gcgtgaacgt actgaattgc cgattggcgc gtatcaggtg agcggtgagt
     1021 acgcgatgat taagttcgcc gcgctggcgg gtgctataga tgaagagaaa gtcgtgctcg
     1081 aaagcttagg ttcaattaag cgtgcgggtg cggatctgat tttcagctac tttgcgctgg
     1141 atttggctga gaagaagatt ctgcgttaat ttttttcctc tcagaaaggg ggagttaacc
     1201 tgttgaaagt acggtgaacg ccccctcacc ctaaccctct ccccaagggg gcgagggaac
     1261 cgtccgcact cgtatcaccc cgcccgataa aacggtttat ccccaaggat cgtcgcccga
     1321 tgcataatcc gccgctgtgg caggtaatcg gcattggcat agtgctgagt cacgcggtta
     1381 tcccaaatcg caatatcatt cggttgccag cgccaacgca cctgaaactc cggtttagtg
     1441 atatgggcga ataaaaagcc taacaaggct tcgctctctt tctcgctgac atcgacaatt
     1501 cgcgtagtaa agccttcatt cacaaacagc gcctgtttac cgctcaccgg atgcgttcgc
     1561 accaccggat gtagcaacgg aggatttttc gcgaccgcct cgcgccagcg ttgatgttcc
     1621 tcctcggttt tgcggtattt gtattccggg aacgatttac ggaaatcatg ctccgcacgc
     1681 agcccgctca gcagctggcg gaagggaaca gagagcgcct cataggccgc aataccgctg
     1741 gtccagagcg tatcaccgcc ggtcgaaggt aactctttag ctgccagaat cgcccctgcg
     1801 ggtggcgttt caataaatgt cacatcggta tgccagttgt cgttatccgg cggattatcg
     1861 ttatgggtat ccagcacgat gatctcgtca accccttcgg catgagggta aacagggtga
     1921 atatgcagtt cgccaaaacg ctgggccagc gcgcgttgct gctgcggcgt aatagcttga
     1981 tcgcgcagaa ataccacctg atagcgcagc accgcatggt aaagctgttc aaactgatta
     2041 tcgcttaacg ggcgcgtcag gtcggcaccc gaaatttgtg cgccgatata cggccccagc
     2101 ggggtaatac tcagacgttc actcattgta cttctccatg ccagggcgtc agacggcgct
     2161 gtaacgcgcg cagacccagt tctaaaagaa aggcgataat cgcaatcacc gcgatccccg
     2221 ccagtaccac gtcagtcgcg agaaattcac ccgctgactg aaccataaat cctaaaccgc
     2281 gtgtcgcggc aatcagctcc gccgctacca gcgtagacca gcccacaccc agaccaatgc
     2341 gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat gacaaaccac agcacctgcg
     2401 cacggctggc acccagcgac tgggcggcac gaatgcgaac ctgctgcgcg cttttcaccc
     2461 ccgccagcac cgacatcgcc accggtgcaa aaatcgctaa atagatcaat aagatcttcg
     2521 aggtttcacc aataccaaac cagatcacca tcagcggcaa ataagccagc ggcggcaccg
     2581 gacgataaag ttcgattacc ggatccagaa tgccgcgcac cgtcgggctt agccccatgg
     2641 cgatcccgac cggaataccg atcaccaccg ccgcgagtag cgccagcaca atgcgcgtca
     2701 ggctggctgc cagatgctgc cacagcgtgg cgtccataaa tccctgcggt ccggcaatgg
     2761 tgagcagttt tgccagtact tgttgcggcg gcggcaaaaa cagcgggcta atcagttgca
     2821 gcgccgccac cgtccaccat acagtgagta aaaccgctaa cgtgccaatg cttaaggtca
     2881 cctgacgcga gagcggccag cgccatttca gccgccgcga atgcagtttt tcattaatga
     2941 gcacactcat gagaacgcct cccgttgctc aaatacgcgg cttaaaacat attcgcgcat
     3001 ggcgatgaat tgtggatcgg acttgatgct gcggctcgac tctcccgcaa caaagcggcg
     3061 agcaaagttg agcggcagcc gctccagcac acggccaggg ccggatgaaa gcagaaccag
     3121 ttcagtcgcc ataaacaccg cttcttctat atcgtgggta atcaacagca cctgcttgcc
     3181 cgtctcctgc cagagtttca gcagcagagt ttgcatctgg tcgcgggtga aggcgtccag
     3241 cgcaccaaac ggttcgtcga gtaataacag ctggggattc gccgccagcg cgcgggcaat
     3301 ccccacccgc tgacgttgac caccggaaag ctgccagata tagcgttttt ctgcgccttc
     3361 cagccccact tttttcagca tctggtgcgc gatttccagt cgctgcattt tctctatacc
     3421 tgccaattgc aggccgaacg ccacgttgtc ctgtacattg cgccacggta gtagcccttc
     3481 attctgaaaa accaccccgc gctctgctcc cggtccctca atacgcttac ccgccagttg
     3541 aatgctgcca tgctgataag gcacaaaacc ggcaatcaga ttcagcaggg tggttttacc
     3601 gcagccggac ggccccagca ccaccagtag ctcgccgctt tccagcgtca ggttgatatc
     3661 ttccagtgcc ggtttgccgc cataatcggc atagagatga gagatttgca gcatctgcgc
     3721 ctccttttat tgcacgaagc gcgaggtaac gtactggctg taatcattcg ctacagccgg
     3781 gaccttgccc tgctctttca aaaactgcgc ggtgtcgatg attgctttgt tcaccggtcc
     3841 ggtcagttct gccgtttgtt gctgcggcgt cagataggta ttccccttca ccagccccgg
     3901 aacgtcacct tccggcacgc cgcttaaacg cgccagtttg ctgatgtttt ccggctgttt
     3961 cagccacgcg tctgggttag caatgtacgg ttgctgagca tcgatggcgc ttttagcgaa
     4021 cgctttcacg acctcaggat gtttctcggc aaaatctttg cgcaccaccc agacgtccag
     4081 cgttggcgca ccccactgcc cgacctgttc agaatcggtc aacaccttgc cgtctttttc
     4141 cagggcgtta accgccggtg cccagacata agcaccatca atatcacccc gctgccatgc
     4201 ggcgataatc gcgggcggct gcaggttcac aatctccact tgccctggtt taatccccca
     4261 gtgtttcagc gccgccagca ggctgtagtg ggtggtggag ataaacggta cggcaatgcg
     4321 tttgccaatc aaatcttcgg gtttgctgat agttttcttt accaccagcg cttcggagtt
     4381 acccagtttt gacgccagca agaagacttc aatcggcacc tgttggctgg ctgcaaccgc
     4441 taatgggctg gaaccgaggt tgccgatttg cacgtcgcca gaagccagcg cccgcacgat
     4501 gctggctccg ctgtcaaact tacgccagtc cacgctcgcc ccgctctctt tagcaaaggt
     4561 gttgtcggcc tgagccactt tcgccggttc ggctgaggtt tgatacgcca cggtgacgtt
     4621 caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca agaagtgtgt tacgcgatga
     4681 aattgccatg attgtctgct cccctgtctt gttatgggag cagtattcag gaataaaaac
     4741 attcattaaa agaattagtc gttatcgcac agatgatttt attcttagca aaaaaacggc
     4801 aaaggaaaga t
//
LOCUS       DAZPJR010000235         4784 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPJR010000235 DAZPJR010000000
VERSION     DAZPJR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.241"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHVB7_004083"
     tRNA            6..81
                     /locus_tag="ACHVB7_004083"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHVB7_004084"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHVB7_004084"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHVB7_004085"
     CDS             complement(299..1057)
                     /locus_tag="ACHVB7_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR1915923.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHVB7_004086"
     CDS             complement(1065..2168)
                     /locus_tag="ACHVB7_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR1915924.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHVB7_004087"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHVB7_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="ACHVB7_004088"
     CDS             complement(3427..4452)
                     /locus_tag="ACHVB7_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1915925.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1311 a         1236 c         1143 g         1094 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gcta
//
LOCUS       DAZPJR010000236         4775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPJR010000236 DAZPJR010000000
VERSION     DAZPJR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.242"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(51..494)
                     /locus_tag="ACHVB7_004089"
     CDS             complement(51..494)
                     /locus_tag="ACHVB7_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1915926.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            651..1580
                     /gene="metA"
                     /locus_tag="ACHVB7_004090"
     CDS             651..1580
                     /gene="metA"
                     /locus_tag="ACHVB7_004090"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR1915927.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1849..3450
                     /gene="aceB"
                     /locus_tag="ACHVB7_004091"
                     /pseudo
     CDS             1849..3450
                     /gene="aceB"
                     /locus_tag="ACHVB7_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3480..4771
                     /gene="aceA"
                     /locus_tag="ACHVB7_004092"
                     /pseudo
     CDS             3480..4771
                     /gene="aceA"
                     /locus_tag="ACHVB7_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1189 a         1257 c         1208 g         1121 t
ORIGIN      
        1 tgcgacgctg gcgcgtcttc tccagcctgg gcaggcacgg caggggcagc ttattcctcc
       61 acatacgcca gattcagcaa cggatacggt ttccccaaat cgtccacctc agagcgtccc
      121 gtaaccttaa aacccacctt cttatagaac ccaaccgcct gctcattttg ctcattaacg
      181 ttggttgtca gttccggtgc catcgagagc gcatgctcca ccagcatccg acccacgccg
      241 cagccgcaca catcaggatc gataaacagc gcatccatat gctgcccact taacaacata
      301 aacccaaccg gctgatcccg ttcattaacc gcgacccaca acggcgcttc cggcaggaag
      361 gaacgaacta gcacctccag ctcggcccga tactctgctg ataaaaaatc gtgtgtggca
      421 tcgacagaac gacaccaaat cgcaacgagt tcctcccctt cctcatgccg tgagcggcga
      481 atactaataa ccattttctc tccttttcgt cattcttata ttctaacgta gccttttcct
      541 tgaaactttc tcaccttcaa catgcaggct cgacattggc aaattttctg gttatcttca
      601 gctatctgga tgtctaaacg tataagcgta tgtagtgagg taatcaggtt atgccgattc
      661 gtgtgccgga cgagctaccc gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga
      721 caacttctcg tgcgtctggt caggaaattc gtccacttaa ggttctgatc cttaacctga
      781 tgccgaagaa gattgaaact gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc
      841 aggtcgatat tcagctgttg cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc
      901 atctgaacaa cttctactgt aactttgaag atattcagga acaaaacttt gacggtctga
      961 tagttaccgg cgcgccgctg ggcctggtgg agtttaatga tgtcgcttac tggccgcaga
     1021 tcaaacaggt gctggagtgg tcgaaagatc acgtcacctc gacgctgttt gtctgctggg
     1081 cggtacaggc cgcgctcaat atcctctacg gcattcctaa gcaaactcgc accgacaaac
     1141 tctctggcgt ttacgagcat catattctcc atcctcatgc gcttctgacg cgtggctttg
     1201 atgattcatt cctggcaccg cattcgcgct atgctgactt tccggcagcg ttgattcgtg
     1261 attacaccga tctggaaatt ctggcagaga cggaagaagg ggatgcatat ctgtttgcca
     1321 gtaaagataa gcgcattgcc tttgtgacgg gccatcccga atatgatgcg caaacgctgg
     1381 cgcaggaata tttccgcgat gtggaagccg gactagaccc ggatgtaccg tataactatt
     1441 tcccgcacaa tgatccgcaa aacaaaccgc gcgcgagctg gcgtagtcac ggtaatttgc
     1501 tgtttaccaa ctggctcaac tattacgtct accagatcac gccatacgat ctacggcaca
     1561 tgaatccaac gctggattaa tcttctgtga tagtcgatcg ttaagcgatt cagcacctta
     1621 cctcaggcac cttcgggtgc cttttttatt tccgaaacgc acctcagcaa gtgaataaat
     1681 tttattcata ttgttatcaa taagttatca agtattttta attaaaatgg aaattgtttt
     1741 tgattttgca ttttaaatga gtagtcttag ttgtgctgaa cgaaaagcgc acaacgatcc
     1801 ttcgttcaca gtggggaagt tttcggatcc atgacgagga gctgcacgat gactgaacag
     1861 gcaacaacaa ccgatgaact ggctttcaca aggccgtatg gcgagcagga gaagcaaatt
     1921 cttactgccg aagcggtaga atttctgact gagctggtga cgcattttac gccacaacgc
     1981 aataaacttc tggcagcgcg cattcagcag cagcaagata ttgataacgg aacgttgcct
     2041 gattttattt cggaaacagc ttccattcgc gatgctgatt ggaaaattcg cgggattcct
     2101 gcggacttag aagaccgccg cgtagagata actggcccgg tagagcgcaa gatggtgatc
     2161 aacgcgctca acgccaatgt gaaagtcttt atggccgatt tcgaagattc actggccccg
     2221 gactggaaca aagtgatcga cgggcaaatt aacctgcgtg atgcagttaa cggcaccatc
     2281 agctatacca atgaagcagg caaaatttat cagctcaagc ccaatccagc ggttttgatt
     2341 tgtcgggtac gcggtctaca cttgccggaa aaacatgtca cctggcgtgg tgaggcaatc
     2401 cccggtagcc tgtttgattt tgcgctctat ttcttccaca actatcaggc actgttggca
     2461 aagggcagcg gtccctattt ctatctgccg aaaacccagt cctggcagga agcggcctgg
     2521 tggagcgaag tcttcagcta tgcagaagat cgctttaatc tgccgcgcgg cgccatcaag
     2581 gcgacgttgc tgattgaaac gctgcccgcc gtgttccaga tggacgaaat ccttcacgcg
     2641 ctgcgcgacc atattgttgg tctgaactgc ggtcgctggg attacatctt cagctatatc
     2701 aaaacgttga aaaactatcc cgatcgtgtc ctgccagaca gacaggccgt gacgatggat
     2761 aaaccattcc tgaatgctta ctcacgcctg ctgattaaaa cctgccataa acgcggtgct
     2821 tttgcgatgg gcggcatggc agcgtttatt ccgagcaaag atgaagagcg taataaccag
     2881 gtgctcaaca aagtaaaagc ggataaagcg ctggaagtca ataacggtca cgatggcaca
     2941 tggatcgctc acccaggcct tgcggacacg gcaatggcgg tattcaacga cattctcggc
     3001 tcccgtaaaa atcagcttga agtattgcgc gaacaagacg cgccgattac tgccgatcag
     3061 ctgctggcac cttgtgacgg tgaacgcacc gaagaaggta tgcgcgccaa cattcgcgtg
     3121 gctgtgcagt acatcgaagc gtggatctcc ggcaacggct gcgtgccgat ttatggcctg
     3181 atggaagatg cggcgacggc tgaaatttcc cgtacctcga tctggcagtg gatccatcat
     3241 caaaaaacgt tgagcaatgg caaaccggtg accaaagcct tgttccgcta gatgctgggc
     3301 gaagagatga aagtcattgc cagcgaactg ggcgaagaac gtttctccca gggccgtttt
     3361 gacgatgccg cacgcttgat ggaacagatc accacttccg atgagttaat tgatttcctg
     3421 accctgccag gctaccgcct gttagcgtaa accaccacat aactatggag catctgcaca
     3481 tgaaaacccg tacacaacaa attgaagaat tacagaaaga gtggactcaa ccgcgttggg
     3541 aaggcattac tcgcccatac agtgcggaag atgtggtgaa attacgcggt tcagtcaatc
     3601 ctgaatgcac gctggcgcaa ctgggcgcag cgaaaatgtg gcgtctgctg cacggtgagt
     3661 cgaaaaaagg ctacatcaac agcctcggtg cactgactgg cggtcaggcg ctgcaacagg
     3721 cgaaagcggg tattgaagca gtctatctgt cgggatggca ggtagcggcg gacgctaacc
     3781 tggcggccag catgtatccg gatcagtcgc tctatccggc aaactcggtg ccagctgtgg
     3841 tggagcggat caacaacacc ttccgtcgtg ccgatcagat ccaatggtcc gcgggcattg
     3901 agccgggcga tccgcgctat gtcgattact tcctgccgat cgttgccgat gcggaagccg
     3961 gttttggcgg tgtcctgaat gcctttgaac tgatgaaagc gatgattgaa gccggtgcag
     4021 cggcagttca cttcgaagat cagctggcgt cagtgaagaa atgcggtcac atgggcggca
     4081 aagttttggt gccaactcaa gaagctattc agaaactggt cgcggcgcgt ctggcagctg
     4141 acgtgacggg cgttccaacc ctactggttg ctcgtaccga tgccgatgca gcggatctga
     4201 tcacctccga ttgcgacccg tatgacagcg aatttattac cggcgagcgt accagtgaag
     4261 gcttcttccg tactcatgcg ggcattgagc aagcgatcag ccgtggcctg gcgtatgcgc
     4321 catatgctga cctggtctgg tgtgaaacct ccacgccgga tctggaactg gcgcgtcgct
     4381 ttgcacaagc tatccacgcg aaatatccgg gcaaactgct ggcttataac tgctcgccgt
     4441 cgttcaactg gcagaaaaac ctcgacgaca aaactattgc cagcttccag cagcagctgt
     4501 cggatatggg ctacaagttc cagttcatca ccctggcagg tatccacagc atgtggttca
     4561 acatgtttga cctggcaaac gcctatgcct agggcgaggg tatgaagcac tacgttgaga
     4621 aagtgcagca gccggaattt gccgctatac cttcgtatct caccagcagg aagtgggtac
     4681 aggttacttc gataaagtga cgactattat tcagggcggc acgtcttcag tcaccgcgct
     4741 gaccggctcc actgaagaat cgcagttcta agcaa
//
LOCUS       DAZPJR010000237         4606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPJR010000237 DAZPJR010000000
VERSION     DAZPJR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.243"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..2162
                     /locus_tag="ACHVB7_004093"
     CDS             <1..2162
                     /locus_tag="ACHVB7_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR1915928.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2396..3679)
                     /locus_tag="ACHVB7_004094"
     CDS             complement(2396..3679)
                     /locus_tag="ACHVB7_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR1915929.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3814..>4606)
                     /locus_tag="ACHVB7_004095"
     CDS             complement(3814..>4606)
                     /locus_tag="ACHVB7_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1915930.1"
                     /translation="QKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKA
                     ASAKLIRSTLSDAFREAIAEGHITTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESS
                     PCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGI
                     SMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELR
                     SLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1093 a         1142 c         1194 g         1177 t
ORIGIN      
        1 aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac
       61 ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga
      121 ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata
      181 actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg
      241 cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata
      301 tgcgtgagat gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt
      361 acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc
      421 ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac
      481 ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa
      541 acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta
      601 ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct
      661 ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact
      721 gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg
      781 ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac
      841 tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc
      901 acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc
      961 aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg
     1021 cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac
     1081 agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg
     1141 caatcatcag aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca
     1201 acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag
     1261 ctaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg
     1321 caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt
     1381 acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa
     1441 ctcagcaacc gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcgctga
     1501 ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg
     1561 aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt
     1621 ttaccctgtc tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg
     1681 aaaatcagcg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     1741 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     1801 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     1861 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     1921 actgggggat gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     1981 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     2041 tgatccatga agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg
     2101 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     2161 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg
     2221 catcttatcc gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc
     2281 atccggcact tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc
     2341 gaaccgtagg ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact
     2401 tcttcgcctc tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt
     2461 tgtagttagt gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat
     2521 tacccgcaaa cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac
     2581 cttcgttgat ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca
     2641 gcgagcggcc cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga
     2701 ggaaaccgta gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag
     2761 tacgtgagtt caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa
     2821 cgatatccac atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg
     2881 tttccagacg gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa
     2941 tgttaacgtt gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt
     3001 tacctgcatc tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc
     3061 cattgttttg tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc
     3121 tctggcagct atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt
     3181 tgacgtcgtg acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca
     3241 catcaatcgg tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat
     3301 ataccgttcc ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct
     3361 tgataattgc cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg
     3421 ccgggccgac aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg
     3481 cagcacctgg tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg
     3541 ccgacagaat cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg
     3601 gcggggttga gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg
     3661 aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga
     3721 agcctgcttt tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca
     3781 acgaacaggt cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc
     3841 cctgcctctg tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg
     3901 ttgagcaaac ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc
     3961 gtgaaaggta ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa
     4021 ataccttgat actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc
     4081 tccgccaaga atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc
     4141 aacatgcaat gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag
     4201 atatccatct acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt
     4261 aacaacagcc agttccattg caagtctgag ccaacatggt gatgattctg ctgtttgata
     4321 aattttcagg tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg
     4381 agtggcagcg accggttttg ttgttatatg gccttcagct attgcctctc ggaatgcatc
     4441 gctcagtgtt gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt
     4501 gagcattgcc gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct
     4561 ccttattgct ttaattttgc tcatgtaatt tatgagtgtc ttctgc
//
LOCUS       DAZPJR010000238         4482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPJR010000238 DAZPJR010000000
VERSION     DAZPJR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.244"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(7..1068)
                     /locus_tag="ACHVB7_004096"
     CDS             complement(7..1068)
                     /locus_tag="ACHVB7_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR1915931.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="ACHVB7_004097"
     CDS             complement(1065..2462)
                     /locus_tag="ACHVB7_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR1915932.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="ACHVB7_004098"
     CDS             2617..3615
                     /locus_tag="ACHVB7_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1915933.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHVB7_004099"
     CDS             complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHVB7_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR1915934.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1098 g         1063 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg cc
//
LOCUS       DAZPJR010000239         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPJR010000239 DAZPJR010000000
VERSION     DAZPJR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.245"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHVB7_004100"
     CDS             complement(<1..105)
                     /locus_tag="ACHVB7_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915935.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHVB7_004101"
     CDS             complement(173..2122)
                     /locus_tag="ACHVB7_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1915936.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHVB7_004102"
     CDS             complement(2207..2542)
                     /locus_tag="ACHVB7_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1915937.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHVB7_004103"
     CDS             complement(2542..2898)
                     /locus_tag="ACHVB7_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1915938.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHVB7_004104"
     CDS             complement(2898..4394)
                     /locus_tag="ACHVB7_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR1915939.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1314 c          970 g         1105 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAZPJR010000240         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPJR010000240 DAZPJR010000000
VERSION     DAZPJR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.246"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(1..1135)
                     /locus_tag="ACHVB7_004105"
                     /pseudo
     CDS             complement(1..1135)
                     /locus_tag="ACHVB7_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1135..1317)
                     /gene="ymfR"
                     /locus_tag="ACHVB7_004106"
     CDS             complement(1135..1317)
                     /gene="ymfR"
                     /locus_tag="ACHVB7_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR1915940.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1329..3062)
                     /locus_tag="ACHVB7_004107"
     CDS             complement(1329..3062)
                     /locus_tag="ACHVB7_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR1915941.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3059..3553)
                     /locus_tag="ACHVB7_004108"
     CDS             complement(3059..3553)
                     /locus_tag="ACHVB7_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR1915942.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3675..4025)
                     /locus_tag="ACHVB7_004109"
     CDS             complement(3675..4025)
                     /locus_tag="ACHVB7_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1915943.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4068..>4409)
                     /locus_tag="ACHVB7_004110"
     CDS             complement(4068..>4409)
                     /locus_tag="ACHVB7_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1915944.1"
                     /translation="RELKLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRAD
                     VAAGRRRLHIKAVCQSVREATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEG
                     AQEYIRTQCIK"
BASE COUNT          948 a         1313 c         1108 g         1040 t
ORIGIN      
        1 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tggaagagta aattccccag
       61 ttgatcccgg tggcgtaggc ttcaaaacgg gacttcatat ccccgcgcag taacgccccg
      121 gcgttaaatt tggcgtaata aacgccctgc ttactttttc gtaccagtcc ggtgttgatc
      181 cgctgttcga tgcgggtcag atacggcacc agtgaatagt tgataaatcc cagccccagc
      241 tcttcgatat tgttgaaggt ggcgcgatcg gtgttctgca ccatgtgcaa cggcacccgg
      301 aacagacgac agatttcttc cagctgaaac ttgcgggttt ccaggaactg actgtcctcg
      361 gcgttcagcg ccatcgactt ccagtccagc cccatttcca gaatcatcgg gcggtgagca
      421 ttgccaagcc ccgtgtgaca ctcctcaaaa tctttcttca gacgctcgta agcatccggc
      481 gtgagctttt gttccgtacg caacacaccg gatgtcaccg caccattact aaacaacctg
      541 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cacgcgcata cgcaatggga
      601 ttcagcccga caagtccatc cagcgtcagg gtgcgcacat gccagatttc attctgggtc
      661 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
      721 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccaccacttc tcccagtgcc
      781 tttaccttgt aggcgtaaaa attcccccgt agacacaggc agacaataac cagctcccag
      841 aattcctgcg gtgtcatgta gccattaggt tttgccgaaa ccaacttatg cagtcgttca
      901 tccaccgccc gtgttttcag ggtaccgctg attttgtaga gactgcaggg cagcatacca
      961 acagactctg ccagcaccct gacgcaagaa tagaccgccg tcaggcgcat ggcccgctgg
     1021 ctactgatcc gctttccggt ataggtgtcg tatgacagcc cgatagcatc agcagttctg
     1081 ctggcgtggt caccggcaca tcgctttttc gttgaaataa tcccgaaaag aacactattt
     1141 acctccgccg acagactgcc gtgtacggtc gagatatcgc gccaccagcc acgaccagaa
     1201 caggcacagc gccccggcaa caacaaaacc cgccggggga taaatcagcc aggcaccata
     1261 cgccagcaaa agcgcaccca gcacgcccac cagaggcgcg agaatcagca tgatcataat
     1321 tacctcagtt aaagcgagcg gatcccgtag gactcaatgt ggtcagacag cgtgtcttct
     1381 ttctcgtaca gcatggctct gccaaccgcc ataatcagcg caactgcacc atcgattttg
     1441 ttttccgcct gctctttgac gggtttcacc acatcatcgt tacccggcat gtttttgccg
     1501 accacgttgc cgatacacca ggtcatgatg ggattgccgt catgatgaaa acgtcccgat
     1561 tcaatcgctg cttccagctc tttcatcgga tcggacatat tggtgaagtt ctggacgata
     1621 gtgacgggat tcaggtcttc atcagcaagg tcatgtgaca acccggtcgc cccgaagggg
     1681 tcgatgggtg actcactgac cgggctgatt ttgttcgccg ctttggcctc ctcgaggatg
     1741 tagcgataat ccacctccgc accatcggta acggtcagaa cgcccatttc cacccatttc
     1801 tgaaagcgtt cggctgtccg tcgatcttca tttttctcga cgctgtacac cgtgtcatac
     1861 ggtacccaga aacgcggggc cacactgtag taatgcgttt taccgtcaat ctcgcgggta
     1921 taaagtcgcg ccatgctgtt catatccagc ttacgcgcca ggtcaaaggc cagaatgcac
     1981 ggctgcccct cgaactgctc aagggtcagt gatttatcct cgcagctctg ccagctcacc
     2041 aagttgaaat acgccgaacg cgccgacacc cagatattga ggtgttttgt tttaaagacg
     2101 tttgccagac gggcgttatt tttcgcacgc tgctgctgac ttaacaaaaa ttcgcgataa
     2161 accgacacgc caatatttgg attggctttt tccagcacct gcgggccggt ccagtcgtca
     2221 ccttcatcaa cggtatagat gatcccgaac agttcatcgt taggcaccga gccgttgagc
     2281 atctcgatga cttcccgccg cttgtcgtag cacggcccct caatgttgta cccggcggtg
     2341 gtgatggccc acatcagtgg ctgacgtcgc gcccccatcc cggtaagcat tgtggtataa
     2401 agcgcatcgg tggcatgctc gtgatattca tcaaccacgg cacagtgggg tgatgaacca
     2461 tcacctgggt tgccgatcag cggttcaaac cgcgcgccat cctccggacg gttcatgttt
     2521 gaggcgttaa cctcaatccc gaacgcttcc gtcagcatgg gtgtgcgttt acacatcagt
     2581 cgcgccgggc gaaagacttc ccacgcctgt ttctctgtcg tggcaccgga atacacttcc
     2641 gcgccaaact cgttatcaca ggcaaaacaa tacagggcaa caccggcaga gattgctgat
     2701 ttgccgttct tacgggggat ttcggtgtac acctcccgga agcggcgcaa ccgggtgcct
     2761 ttattgaccc agccaaacgc acagcagatc acaaatagct gccacggctc cagcgtgatg
     2821 ggcatccgtt tgaatgccca ctcccccttg gtgtgcggca acagctgaat aaatttcgcg
     2881 gcccgttcag ccaggtcctt gtcgaagcgg taacgaaacg acttactttt ttccgccatc
     2941 aggtcatcaa gatggcgctg gcaggcctga atcacaaact ggcaggcaac aatctttccg
     3001 cgcacgacat cccgggcata ctgatttgca gcatttacgt tggggtaaga tttccggctc
     3061 atgattcgat aattttcaga aacgggttag tggctttctt ctgccccgcc aggccaatca
     3121 gacgctggcg gctgctgggg tcgagtccga gcattgcccc cgtgctgctc atctcggact
     3181 cctgttcttt tttggcggtc agctccggat ttttgaccct gccgcccatt gcaccggtga
     3241 tggtgttgcc ctgtatggca atatttttca cggcacgtcg ccagaactca taggccacgc
     3301 accaccgctc aagtaccgcc aggtcagtca cgcacagcag gccctgaccg cagagttctt
     3361 tggttgtcag ttgccacatg atcgtggcga gagggagatc ttcttcagcg aaccactctg
     3421 gtggctcaac acctttgatg ggcgtaaaaa caggttcatc tttgttcagg gctcgcttgc
     3481 cggggtttcc ggccagcgcc ttgcgcgccg ttggcttggg gcgacgcccg gaacgccccg
     3541 ccgttccagc catatgcggc actcctggtt aaatttcatt tttcgcgggt ataaaaaaac
     3601 gatggggcgg gcagtccgga agacgtcagg tcacagggat ttgccccgcc cctccctaca
     3661 agtgagaata attatcaccg gattcgttcg cgcgctgttt tcgctttatg gcagggccag
     3721 cacagactct gcaggttgct gtctgcgtct gttccgccat gcgctttcgg gatgatgtgg
     3781 tcgacggttt tcgcctcgct caccacaccg acacgcagac acaactgaca cagaccttta
     3841 tcgcgcttca gaatacgggc acgaatcacc gtccattttg agccatagcc acgctggtgg
     3901 cggctcagtc cgcgctggtg ctgcacccag ccttcaccgc gatgtttatc gcagtaaccc
     3961 gaactgtctg tggttgtacc tgcacatcca cgcttacggc aggcacgtgg gattagtgat
     4021 ggcataaata cctcataccc tgcgaaatgt ttaccacgat aaaaaggcta cttaatgcac
     4081 tgagtgcgga tatactcctg tgccccttcc agttgcatct gcatcgtcat cagccgctct
     4141 ctgagggtga aataatcccg ttcagcggtg tctgccagtc gggggctggt tgcattatcc
     4201 acgccggagg cggtggtggc ttcacgcact gactgacaga ctgctttgat gtgcaaccga
     4261 cgacgaccag cggcaacatc agcgcgcaga gtttcatttt cagctttcgc atcagctaac
     4321 tccttcgtgt atttagcatc gagtgcatta acatcacgct gtctctgctg catgtcagta
     4381 atggttgcgt tcgccagctt cagttctct
//
LOCUS       DAZPJR010000241         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPJR010000241 DAZPJR010000000
VERSION     DAZPJR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.247"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(21..1967)
                     /locus_tag="ACHVB7_004111"
     CDS             complement(21..1967)
                     /locus_tag="ACHVB7_004111"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1915945.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SADMTFRVKPESLILTIFFLGLIP"
     gene            complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHVB7_004112"
     CDS             complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHVB7_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR1915946.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2369..3076
                     /locus_tag="ACHVB7_004113"
     CDS             2369..3076
                     /locus_tag="ACHVB7_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR1915947.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="ACHVB7_004114"
     tRNA            3182..3257
                     /locus_tag="ACHVB7_004114"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..>4409
                     /locus_tag="ACHVB7_004115"
     CDS             3455..>4409
                     /locus_tag="ACHVB7_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1915948.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1149 a         1162 c         1057 g         1041 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZPJR010000242         4408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPJR010000242 DAZPJR010000000
VERSION     DAZPJR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.248"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHVB7_004116"
     CDS             complement(<1..590)
                     /locus_tag="ACHVB7_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1915949.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHVB7_004117"
     CDS             complement(583..993)
                     /locus_tag="ACHVB7_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR1915950.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHVB7_004118"
     CDS             complement(999..1532)
                     /locus_tag="ACHVB7_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1915951.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2598)
                     /locus_tag="ACHVB7_004119"
     CDS             complement(1510..2598)
                     /locus_tag="ACHVB7_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR1915952.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLGENILSCDTERSIRERFSEYQVSGQRPG
                     NDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRE
                     TTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGP
                     ADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2595..3986)
                     /locus_tag="ACHVB7_004120"
     CDS             complement(2595..3986)
                     /locus_tag="ACHVB7_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR1915953.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4033..>4408)
                     /locus_tag="ACHVB7_004121"
     CDS             complement(4033..>4408)
                     /locus_tag="ACHVB7_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1915954.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT         1056 a         1286 c         1041 g         1025 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcaccagc gccgtcgccg ctttacctgt tccgggtttt cccagaacca gtcgcccgca
     2101 ttcatcgtca taagccagcg tctgaacctg ccccagtaac cggttaagac aatcggcaac
     2161 ggtttcgccg tgttccggct gggcatcaat caccgctgtc tgcggcacgc cagcatcaac
     2221 aacggtgatg ccaaatggcg cagccagttc actgactatt ctgagcaggt tttttccgct
     2281 ctgctggagt ggcaaagcag agcagtcaac cagatcggct gttttgcttc gcccgacaat
     2341 tcccatgctg acgctgctgg cgtcataacg aagcggtagt gcctccacat atccggtgag
     2401 cacaggctca tccccgataa gcacttcaac cagctcacca ttttttatcc ggggttgata
     2461 atcccggctt ccgggccagc gggtggtaat ggcaacatta aaatcccggg caatacggtt
     2521 aatgcccgca ctgatacgga cggatgtcca gccgccccat tcgcgaccgg aaacccgaag
     2581 taaaacggta tcattcatct gacgggtacc cttaatgccc tgaccggaac aaagcccgga
     2641 tgagagatgg catttcgatc caggatatca gtttcacggg acgcatcgtc gtaccatgac
     2701 gcagccagaa caagcgcagg cagaacttcc gctggcgttc gctctgcggt ttcctccgtc
     2761 tgaaccagac gcgcctgaat atcccggttc agttctgtac gtaatgacgt cagctgaaaa
     2821 aacagcctgt catccgccgt acgcctgagc tcctgttcaa tcgccgcgtt cagcgatttg
     2881 cgtatgatgg tgagattttc ccgcgtgggt ggttgcgctc tctcatcctg tcctgtgctg
     2941 gctgccacgc tgtcaagtgc cggatgagaa acgtgaataa tatcggactg acgttcagtg
     3001 gaaccaccaa cagccacaac cgcctgctga tttttcacca ggcttccggg ttgtggcagt
     3061 gaagtgaccg cccttgtggc ttcgctgatt gccgtcgtcc ggatgacagc agccacaagg
     3121 tttgtctgct gtttttgcct gataaccgat gcggaatctg tgggccacac cgcacgcggt
     3181 gccagtcctg ggtccagcgt aatgccggac attgtcgtta tggactgcac cagatcctgt
     3241 gtgttatcca ccagccgggt tccggcccgc caggtatcct gcaacgtatg cacaaaatca
     3301 ctggcaaccg acggcggcat cagaatgacg gataaatcgc cctgcagtag tctcgctccg
     3361 gcagaaacag ccgaattaac cattttgaaa gctgtctgaa cggttcccag catatcggtc
     3421 gtccgggcaa taacatcgtt ctgaataaag tccggtattc ccgcgagatc aaaatcgccg
     3481 aacatgtctt caatcaactc atccaggaat acggacgact cctccagttt tctggcggtt
     3541 gcagctccgg cgaccggaaa tgacagttcc ccgctctcaa caaactgaaa tgaaacccgg
     3601 cacatacggc cttcagtgcc ggagtgagaa acggtcacct gcccgtcaat acagccctgc
     3661 atttcaccga actgcggatg gatcagcgtc cccggcccgg cggtttcaat cgctgtaatc
     3721 agcctgtcgc gctgctctgc gtaatcatca ccaacgagat acgcattaat cgtcagccgt
     3781 cgcgtggcac gcccgagatc ttccgtgtac ggtttgtcac ggtttgggta ttcatggacc
     3841 tgaacgcggc gtccgaacga accctcgtcg ctctccaccg aaaacggaac gccacgaaat
     3901 gaagcgtcgt aaagattatc gcgccaattg atacctgaag atgcagaaaa gagtgaagaa
     3961 agagaaggta aagaaggtaa gtccatactg tctccactat aaaaaaaccg ccaatttctg
     4021 gcggcttata tattaatcat cattaatatc tttcatattc aatttgcaat tttcgtaggt
     4081 cactcccgac tcgccattaa cattaactcc agctctgata ttcccgtttt cttttacatc
     4141 tacaaaaaac tggcctcttg gcatcttgaa cgcaaatgta aaaccaacag tatctgaatc
     4201 aaaatcatca tctttatata ttgggatcaa aaatgtcatc gtcgctggtg atgcattgtt
     4261 tgcaaaatca aatacaccta tacttttgcc gaattcataa gaagcttttc tcaatataat
     4321 cgcaccgctg gcagaagggg atgggcacga tatcctcagt tgtctgaatc catctgtatc
     4381 caagtcaacg tcattatctg cagatgtt
//
LOCUS       DAZPJR010000243         4404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPJR010000243 DAZPJR010000000
VERSION     DAZPJR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.249"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHVB7_004122"
     CDS             complement(139..3654)
                     /locus_tag="ACHVB7_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR1915955.1"
                     /translation="MDKNALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="ACHVB7_004123"
     CDS             complement(4106..>4404)
                     /locus_tag="ACHVB7_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1915956.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1127 c          905 g         1231 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DAZPJR010000244         4330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPJR010000244 DAZPJR010000000
VERSION     DAZPJR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.250"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            147..3239
                     /gene="ebgA"
                     /locus_tag="ACHVB7_004124"
                     /pseudo
     CDS             147..3239
                     /gene="ebgA"
                     /locus_tag="ACHVB7_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
     gene            3236..3685
                     /locus_tag="ACHVB7_004125"
     CDS             3236..3685
                     /locus_tag="ACHVB7_004125"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR1915957.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKMVLKVTIEDGYFHNK"
     gene            3748..>4330
                     /locus_tag="ACHVB7_004126"
     CDS             3748..>4330
                     /locus_tag="ACHVB7_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1915958.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
BASE COUNT         1044 a         1167 c         1089 g         1030 t
ORIGIN      
        1 acagtgctgc cgtttatttt cgtgatccag ttaaagtaaa tgcatttacc tgtcactttt
       61 tagtaaaaat tttactaaac tccccagcaa ttacacaaac taccatcacc atgaatggtt
      121 ccgatttctc tctaccggga ggccctatga atcgctggga aaacattcag ctcacccacg
      181 aaaaccgact tgcgccacgt gcgtactttt tttcatatga ttctgttgcg caagcgcgta
      241 cctttgcccg cgaaaccagc agcctgtttc tgcccttaag cggtcagtgg aatttccact
      301 tttttgacca cccgctgcaa gtgcctgaag ccttcacctc tgagttaatg gctgactggg
      361 ggcatattac cgtccccgcc atgtggcaaa tggaaggtca cggcaaactg caatataccg
      421 acgaaggttt tccgttcccc atcgatgtgc cgtttgttcc cagcgataac ccaaccggtg
      481 cctatcaacg tattttcacc ctcagcgacg gctggcaggg taaacagacg ctgattaaat
      541 ttgacggcgt cgaaacctat tttgaagtct acgttaacgg tcagtatgtg ggtttcagca
      601 agggcagtcg cctgaccgca gagtttgaca tcagcgcgat ggttaaaacc ggcgacaacc
      661 tgttgtgtgt gcgcgtgatg caatgggcgg actctaccta cgtggaagac caggatatgt
      721 ggtggtcagc ggggatcttc cgcgatgttt atctgatcgg aaaacaacta acacatatta
      781 acgatttcac cgtgcgtacc gactttgacg aagcctattg cgatgccacg ctttcctgcg
      841 aagtggtgct ggaaaatctc gccgcctccc ctgtcgtcac gacgctggaa tataccctgt
      901 ttgatggcga acgcgtggtg cacagcagcg ccattgatca tttggcaatt gaaaaactga
      961 ccagcgccag ctttgctttt actgtcgaac agccgcagca atggtcagca gaatcccctt
     1021 atctttacca tctggtcatg acgctgaaag acgccaacgg caacgttctg gaagtggtac
     1081 cacaacgcgt tggcttccgt gatatcaaag tgcgcgacgg tctgttctgg atcaataacc
     1141 gttatgtgat gctgcacggc gtcaaccgtc acgacaacga tcatcgcaaa ggccgcgccg
     1201 ttggaatgga tcgcgtcgag aaagatctcc agttgatgaa gcagcacaac atcaactccg
     1261 tgcgtaccgc tcactacccg aacgatccgc gtttttacga actgtgtgat atctacggcc
     1321 tgtttgtgat ggcggaaacc gacgtcgaat cgcacggctt tgctaatgtc ggcgatatca
     1381 gccgtattac cgacgatccg cagtgggaaa aagtctacgt cgagcgcatt gttcgccata
     1441 ttcacgcgca gaaaaaccat ccgtcgatca tcatctggtc gctgggcaat gaatccggct
     1501 atggctgtaa catccgcgcg atgtaccacg cagcgaaggc gctggatgac acgcgactgg
     1561 tgcattacga agaagatcgc gatgctgaag tggtcgatat tatttccacc atgtacaccc
     1621 gcgtgccgct gatgaatgag tttggtgaat acccgcatcc gaagccgcgc atcatctgtg
     1681 aatatgctca tgcgatgggg aacggaccgg gcgggctgac ggagtaccag aacgtcttct
     1741 ataagcacga ttgcattcag ggtcattatg tctgggagtg gtgcgaccac gggatccagg
     1801 cacaggacga ccacggcaat gtctggtata aattcggcgg cgactacggc gactatccca
     1861 acaactataa cttctgtctt gatggtttga tctattccga tcagacgccg ggaccgggcc
     1921 tgaaagagta caaacaggtt atcgcgccgg taaaaatcca cgcgcgggat ctgactcgcg
     1981 gcgagttgaa agtcgaaaat aaactgtggt ttaccacgct tgatgactac cccctgcacg
     2041 cagaggtgcg cgccgaaggt gaaacgctcg cgacgcagca gattaaactg cgcgacgttg
     2101 cgccgaacag cgaagccccc ttgcagatca cgctgccgca gctggacgcc cgcgaagcgt
     2161 tcctcaacat tacggtgacc aaagattccc gcacccgcta cagcgaagcc gggcattcta
     2221 tcgccactta tcagttcccg ctgaaggaaa acaccgcgca gccagtgcct ttcgcaccaa
     2281 ataatgcgcg tccgctgacg ctggaagacg atcgtttgag ctgcaccgtt cgcggctaca
     2341 acttcgcgat caccttctca aaaatgagtg gcaaaccgac atcctggcag gtaaatggcg
     2401 agtcgctgct gacccgcgag ccaaagatca acttcttcaa gccgatgatc gacaaccaca
     2461 agcaggagta cgaagggttg tggcagccga atcatttgca gatcatgcag gaacatctgc
     2521 gcgactttgc cgtagaacag agcgagggtg aagtgttgat catcagccgc acggttattg
     2581 ccccgccggt gtttgatttc ggaatgcgct gcacctacat ctggcgcatc gctgccgatg
     2641 gccaggttaa cgtggcgctt tccggcgagc gttacggcga ctatccgcac atcattccgt
     2701 gcatcggttt caccatggga attaacggcg aatacgatca ggtggcgtat tacggtcgtg
     2761 gaccgggcga aaactacgcc gacagccagc aggctaacat catcgatatc tggcgcagca
     2821 ccgtcgatgc catgttcgag aactatccct tcccgcagaa caacggtaac cgtcagcatg
     2881 tccgctggac ggcactgact aaccgccacg gtaacggtct gctggtggtt ccgcagcgcc
     2941 caattaactt cagcgcttgg cactatacct aggaaaacat ccacgctgcc cagcactgta
     3001 acgagctgca gcgcagtgat gacatcaccc tgaacctcga tcaccagctg cttggcctcg
     3061 gctccaactc ctggggcagc gaggtgctgg actcctggcg cgtctggttc cgtgacttca
     3121 gctacggctt tacgttgctg ccggtttctg gcggagaagc taccgcgcaa agcctggcgt
     3181 cgtatgagtt cggcgcaggg ttcttttcca cgaatttgca cagcgagaat aagcaatgag
     3241 gatcatcgat aacttagaac agttccgcca gatttacgcc tctggcaaga agtggcaacg
     3301 ctgcgttgaa gcgattgaaa atatcgacaa cattcagcct ggcgtcgccc actccatcgg
     3361 tgactcattg acttaccgcg tggagacaga ctccgcgacc gatgcgctat ttaccgggca
     3421 tcgacgctat tttgaagtgc attactacct gcaagggcag caaaaaattg aatatgcgcc
     3481 gaaagagaca ttacaggtag tggaatatta tcgtgatgaa actgaccgtg aatatttaaa
     3541 aggctgcgga gaaaccgttg aggtccacga agggcaaatc gttatttgcg atatccatga
     3601 agcgtatcgg tttatctgca ataacgcggt caaaaaaatg gttctcaaag tcaccatcga
     3661 agatggttat ttccataaca aataacaact acggcggcaa aaggagtttg ccgccaccgc
     3721 taccctactc attttcggag atgtgttatg tctgatacca aacgtaatac aatcggcaaa
     3781 ttcggcttgc tctcgctgac ttttgccgcc gtttacagct ttaacaacgt tatcaataat
     3841 aatattgatc ttggactggc ctcggcaccg atgtttttcc tcgcgacgat tttttatttt
     3901 attcccttct gtctgatcat cgcagaattt gtttcgttaa ataaaaactc agaagccggt
     3961 gtctacgcat gggtaaaaag ttcgctgggc ggacgttggg catttattac tgcctatacc
     4021 tactggttcg taaacctgtt ctttttcacc tcactgttgc cgcgcgttat tgcttatgct
     4081 tcgtatgcct tcctcggata cgaatatatt atgacgccgg ttgccaccac cattatcagt
     4141 atggtgctgt tcgccttctc cacctgggtt tccaccaacg gggcgaaaat gttggggcca
     4201 attacctccg tcacttcaac gctgatgctg ctgttaacgc tctcctacat tttactggca
     4261 ggtacggcgc tggttggcgg cgtacagcct gctgacgcca tcaccgttga cgcgatgatc
     4321 ccgaacttca
//
LOCUS       DAZPJR010000245         4262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPJR010000245 DAZPJR010000000
VERSION     DAZPJR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.251"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            288..896
                     /locus_tag="ACHVB7_004127"
     CDS             288..896
                     /locus_tag="ACHVB7_004127"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR1915959.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            994..2385
                     /gene="selA"
                     /locus_tag="ACHVB7_004128"
     CDS             994..2385
                     /gene="selA"
                     /locus_tag="ACHVB7_004128"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR1915960.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2382..4226
                     /gene="selB"
                     /locus_tag="ACHVB7_004129"
     CDS             2382..4226
                     /gene="selB"
                     /locus_tag="ACHVB7_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR1915961.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          964 a         1078 c         1241 g          979 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ctgccggttt tccgctcatc
       61 tgctcatcct tgttttatcg ggttatttgt ctgcttccag gcaaaaccct acagatttat
      121 aagaaagcct taagtgagat atccatcaga tagaaacaat taggaaaata tttatatcac
      181 catgataaat atgtgattta tttggaagta acaaaccatc aacattttct gccctttttc
      241 ccatcaccag gccatactga tatttttcct cgcacaaagg actcggcatg aaactcgtcg
      301 gtagctacac cagcccgttt gtacgcaaac tttctattct gttgttagaa aagggcataa
      361 ctttcgaatt tattaatgaa ctgccctata acgcggacaa cggcgtggcg caatttaacc
      421 cgttaggaaa agtgccggtg ctggtgaccg aagagggcga atgctggttt gattcgccga
      481 tcatcgctga atatattgag ttaatgaatg tcgctccggc gatgttgccg cgcgatccgc
      541 tggagtcgtt gcgggtgcgc aaaattgagg cgctggcgga tggcattatg gatgccgggc
      601 tggtatcggt gcgtgaacag gcgcgtccgg cggcgcagca gtctgaagat gaattgttac
      661 gccagcggga gaaaatcaac cgcagtctgg atgtgctgga aggatatctg gtcgatggca
      721 cactcaaaac cgatacggtc aatctggcga ctatcgccat tgcctgtgct gtcggatatc
      781 tcaatttccg ccgcgttgcg ccaggttggt gtgtcgaccg cccacactta gtcaaactgg
      841 tcgaaaacct gtttagccgc gaaagttttg ctcgcaccga accgccaaag gcttgatgcg
      901 cgatatgtcc tcctgaccca tctcacgtta caatccgtgg ttatgttaaa cgcccttctc
      961 cgtgtgagag ggccttgatc agccaggttt cctatgacaa ccgaaacgcg ttccctctat
     1021 agtcaacttc cggctattga tcgcttattg cgcgatagct ccttcctttc tttgcgtgat
     1081 acttatggtc acacccgcgt ggtggaattg ttgcgtcaga tgctcgacga agcgcgagaa
     1141 gtgattcgtg acagccagac gcttcctgcg tggtgtgaaa actgggcgca agaagtcgat
     1201 gcccggttga cgaaagaggc gcagagcgcg ctgcgtccgg tgatcaacct gacgggaacc
     1261 gtgctgcata ccaaccttgg acgagcttta caggcggaag ccgcggtgga agccgttgcg
     1321 caggctatgc gttcgccagt gaccctcgag tacgatctgg acgacgccgg acgcggacat
     1381 cgcgatcggg cgctggcgca actgctgtgc cgtattacgg gggcggaaga tgcctgtatc
     1441 gtcaataaca atgcggcggc ggtgttattg atgttggcgg ccactgccag cgggaaagag
     1501 gtggtagtat ctcgcggcga attggtggag attggcggcg cgtttcgtat tcccgatgtt
     1561 atgcgtcagg caggctgcac cctacacgaa gtagggacca ccaaccgcac gcacgcgaat
     1621 gattatcgtc aggcggtgaa tgaaaatacc gcactgttga tgaaagtaca taccagtaac
     1681 tacagcattc aggggttcac caaagcgata gatgaagcgg aactggtggc gctcggcaaa
     1741 gagctggatg ttcccgtagt gactgattta ggcagtggct cgctggtcga tcttagccag
     1801 tacggtttgc cgaaagagcc aatgccgcag gagttgattg cggcgggcgt cagtctggtg
     1861 agcttctccg gcgacaagtt gttaggcggg ccgcaggcag ggattatcgt tggtaaaaaa
     1921 gagatgatcg cccgcctgca aagccacccg ctgaagcgtg cattacgcgc ggataaaatg
     1981 accctcgcgg cgctggaagc cacgttgcgt ctttatttac accctgaagc tctgagtgaa
     2041 aaattaccga ccctgcgcct gcttacccgc agcgcagagg tcattcaaat ccaggcacaa
     2101 cgtttacagg ctccccttgc cgcacattac ggcgcggagt ttgcggtaca ggttatgcca
     2161 tgtctttcgc agattggcag tggttcgctg ccggttgatc gcctgccgag cgcggcatta
     2221 acgtttacac cccatgatgg acgcggtagc caccttgagt cattagccgc ccgctggcgt
     2281 gaattgccag tgccgatgat tggtcgtatt tatgacggac gattgtggct ggatttacgc
     2341 tgccttgaag atgagcaacg gtttttggag atgttgttga aatgattatt gcgactgccg
     2401 gacacgttga ccacggcaaa acaaccttat tgcaggtgat tactggcgta aatgctgacc
     2461 gtctgccgga agaaaaaaag cgcggcatga ccatcgatct cggctatgcc tactggccgc
     2521 agccggatgg tcgcgtgcct agttttatcg acgtacccgg tcatgaaaag tttctttcca
     2581 acatgctggc gggcgttggt ggtatcgatc acgcgctgtt ggtggtggcg tgcgatgacg
     2641 gcgtgatggc gcagacccgt gagcatctgg cgattttgca gctgaccggt aacccgatgc
     2701 tgacagtggc gctgaccaaa gccgatcgcg tggacgaagc gcgtgttgat gaggttgaac
     2761 gccaggtaaa ggaggtgctg cgggaatacg gttttgctga ggcaaaactg tttatcaccg
     2821 cagcaaccga aggtcgggga attgatgccc tgcgcgagca tctgcttcag ttaccaggac
     2881 gtgaacacgc cagccaacat agtgtccgcc tcgcgatcga ccgcgcattt accgtaaaag
     2941 gtgccggtct ggtcgtcacc ggtacggcgt taagcgggga agtgaaggta ggcgattcac
     3001 tctggctgac tggtgtaaat aaaccgatgc gtgtacgtgc gctgcatgca caaaaccagc
     3061 caacagaaac cgcccatgcc gggcagcgta tcgcgcttaa catcgcgggt gatgcggaaa
     3121 aagagcagat taaccgtggc gactggctgc ttgccgatgc gccgccagag ccgttcacac
     3181 gggtgattgt cgagcttcaa acccatacgc cgctgaccca gtggcagccg ctgcatattc
     3241 accacgccgc cagccacgtt acgggacgcg tttcgctgct ggaagataac cttgccgagc
     3301 tggtgttcga cacgccgctg tggctggcgg ataacgatcg cttagtgctg cgcgatatct
     3361 cggcccgcaa cacgctggcg ggggcgcgtg tggtgatgct taacccgccg cgtcgcggta
     3421 aacgtaagcc ggaatatctg caatggctgg cgtctcttgc acgggcgcag agcgatgccg
     3481 atgcgttatc tgttcatctg gaacgcggcg cggttaacct tgcggatttc gcctgggcgc
     3541 gccagctcaa cggcgaaggg atgcgcgaat tgctgcaaca gcctggctat attcaggctg
     3601 gttatagctt gttgaatgcg ccggttgccg cccgctggca gcggaaaatt ctcgacacat
     3661 tagcgactta tcatgagcaa catcgcgatg aacctggtcc tgggcgcgaa cgtctgcgac
     3721 gtatggcgtt gccaatggaa gatgaagcgc tggtactgtt gctgattgaa aagatgcgcg
     3781 aaagcggcga catcctcagt catcacggtt ggctgcatct gccggatcac aaagcgggct
     3841 tcagcgaaga gcagcaggcc atctggcaaa aagcagagcc gctgtttggt gacgagccgt
     3901 ggtgggtgcg tgatctggca aaagagacgg gtaccgacga gcaggcaatg cgcctgacgc
     3961 tacgccaggc ggcgcagcaa ggaataatta ccgcgatcgt caaagatcgt tattaccgta
     4021 acgatcggat tgtcgaattt gccaatatga tccgcgatct cgatcaggag tgtggttcaa
     4081 cctgcgcggc ggatttccgc gatcgcttag gcgtaggccg aaagctggca attcagattc
     4141 tggaatattt tgaccgcatt ggctttacgc gtcgtcgtgg aaatgatcat ttattgcgcg
     4201 acgcattatt atttccggaa aaataaggaa atgattaatt aatttttaaa ataaattaat
     4261 ac
//
LOCUS       DAZPJR010000246         4192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPJR010000246 DAZPJR010000000
VERSION     DAZPJR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.252"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHVB7_004130"
     CDS             complement(<1..215)
                     /locus_tag="ACHVB7_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1915962.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHVB7_004131"
     tRNA            complement(330..405)
                     /locus_tag="ACHVB7_004131"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHVB7_004132"
     tRNA            complement(412..486)
                     /locus_tag="ACHVB7_004132"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHVB7_004133"
     tRNA            complement(603..687)
                     /locus_tag="ACHVB7_004133"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHVB7_004134"
     tRNA            complement(696..771)
                     /locus_tag="ACHVB7_004134"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHVB7_004135"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHVB7_004135"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR1915963.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHVB7_004136"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHVB7_004136"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR1915964.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHVB7_004137"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHVB7_004137"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR1915965.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1040 c          936 g         1136 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatct tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 acttcccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAZPJR010000247         4187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPJR010000247 DAZPJR010000000
VERSION     DAZPJR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.253"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..325
                     /locus_tag="ACHVB7_004138"
     CDS             <1..325
                     /locus_tag="ACHVB7_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1915966.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            552..1817
                     /locus_tag="ACHVB7_004139"
     CDS             552..1817
                     /locus_tag="ACHVB7_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1915967.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1829..2695
                     /gene="ydiB"
                     /locus_tag="ACHVB7_004140"
     CDS             1829..2695
                     /gene="ydiB"
                     /locus_tag="ACHVB7_004140"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR1915968.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2726..3484
                     /gene="aroD"
                     /locus_tag="ACHVB7_004141"
     CDS             2726..3484
                     /gene="aroD"
                     /locus_tag="ACHVB7_004141"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR1915969.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3628..>4187
                     /locus_tag="ACHVB7_004142"
     CDS             3628..>4187
                     /locus_tag="ACHVB7_004142"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR1915970.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
BASE COUNT         1093 a          938 c         1005 g         1151 t
ORIGIN      
        1 attttatgtg gtgataatat ttgcttttgt cattggtttt acctctgctg gaggtgttgt
       61 gcaaattggc ctgacgttaa tggctgaacg tttcccttac gctaaaggta aagctacagg
      121 gatctattac agtgcgggca gtattgcgac ctttactatt ccgttgatta cggctcatct
      181 gtcccaaaga agtattgccg atattatgtg gttcgatacc gccatcgctg ccatcggttt
      241 tttactggca ctgtttatcg gcttacgcag ccgcaaaaaa acgcggcatc actcgctaaa
      301 ggaaaatgtc gctccgggtg ggtaatgcaa tattcttttc aggtcatgca agatcttacg
      361 gataaataac tctttctgcg ctaactaagg aaaatcgcga tcaaaaacaa actatgacat
      421 gcaatattcc tggaaacata aactttatgc catgtaccca gggaaaatca tcttcagtat
      481 agtaattatg taaaccgtcg gagaacaata cgtacggtaa cgaaattatc tttcagcaag
      541 gagctgtgaa aatgtctcaa aataaggctt tcagcacgcc atttatcctg gctgttcttt
      601 gtatttactt cagctatttc ctgcacggca ttagtgttat tacgcttgcc caaaatatga
      661 catctctggc agaaaagttt tccactgaca acgcgggcat tgcctattta atttccggta
      721 tcggtttggg gcgattgatc agtattttat tcttcggtgt gatctccgat aagtttggtc
      781 gtcgggcggt gatattaatg gcagtaataa tgtatctgct attcttcttt ggtattcccg
      841 cttgcccgaa tttaactctc gcctacggtc tggcagtgtg cgtaggtatc gctaactcag
      901 cgctggatac gggtggctac cccgcgctca tggaatgctt tccgaaagcc tctggttcgg
      961 cggtcatact ggttaaagcg atggtgtcat ttgggcaaat gttctaccca atgctggtga
     1021 gctatatgct gctcaataat atctggtacg gctatgggct gattattccg ggtattctgt
     1081 ttgtactgat cacgctgatg ctgttgaaaa gcaaattccc cagccagttg gtggacgcca
     1141 gcgtagctaa tgaattaccg caaatgaaca gcaaaccgtt agtctggctg gaaggtgttt
     1201 catcggtact gttcggtgta gccgcattct cgacctttta tgtgattgtg gtgtggatgc
     1261 ccaaatatgc gatggctttt gctggtatgt cagaagctga ggcattaaaa accatctctt
     1321 attacagtat gggctcgttg gtctgtgtct ttatttttgc cgcactactg aaaaaaatgg
     1381 tccggcccat ctgggctaat gtatttaact ctgcgctggc aacaataaca gctgccatta
     1441 tctacctgta cccttctcca ctggtgtgta atgccggcgc ctttgttatc ggtttctcag
     1501 cagctggcgg cattttacag ctcggtgttt cggtcatgtc agagtttttc cccaaaagca
     1561 aagccaaagt caccagtatt tatatgatga tgggtggact ggctaacttt gttattccgc
     1621 tgattaccgg ttatctgtcg aacatcggcc tgcaatatat cattgttctc gattttactt
     1681 tcgcgctgct ggccctgatt accgcaatta ttgtttttag ccgctattac cgcgttttta
     1741 ttattcctga gaatgatgtg cggtttggcg aacgcaaatt tagcacccgg ttaaacacaa
     1801 ttaagcatag aggttaaagg agttaattat ggatgttacc gcaaaatacg aattgattgg
     1861 gttgatggct tatcctatcc gccacagttt atcgcctgaa atgcagaata aagccttaga
     1921 aaaagcggga ttgccattta cctatatggc cttcgaagtg gataacgata gctttcccgg
     1981 agcaattgaa ggattaaaag ccctcaaaat gcgcggaact ggtgtgtcga tgccgaacaa
     2041 acaactggcg tgtgaatatg ttgatgaatt aacgccagca gccaaactgg tgggtgccat
     2101 caacaccatc gttaatgatg atggctatct gcgtggctat aacaccgacg gcacgggcca
     2161 tattcgcgcc attaaagaga gcggttttga tatcaaaggc aaaacgatgg tgctgttagg
     2221 ggccggaggt gcctcaacag cgattggcgc gcagggggca attgaaggtt taaaagaaat
     2281 taaactcttt aaccgtcggg atgagttctt cgataaagcc ctcgccttcg cgcagcgggt
     2341 taacgaaaac accgattgtg tcgtcacggt taccgatctc gccgatcagc aagcctttgc
     2401 tgaagtcctg gcttccgccg acattttaac caatggcaca aaagtaggta tgaaccccct
     2461 tgagaatgaa tcattggtta atgatatcag tctgttacat ccgggactgc tggtcactga
     2521 atgcgtgtat aacccgcata tgacgaagtt attgcagcag gcacaacaag caggttgcaa
     2581 aacgattgat ggatacggca tgttgttgtg gcaaggggct gaacagttca cgttatggac
     2641 tggcaaagat ttccctctgg aatatgttaa acaggtcatg gggttcggtg cctgacaggc
     2701 tgaccgcgtg cagaaagggt aaaaaatgaa aaccgtaact gtaaaagatc tcgtcattgg
     2761 tgcgggcgca cctaaaatca tcgtctcgct gatggcgaaa gatatcgccc gcgtgaaatc
     2821 cgaagctctc gcctatcgtg aagcggactt tgatattctg gaatggcgtg tggaccactt
     2881 tgccgacctc tccaatgtgg agtctgtcat ggcggcggca aaaattctcc gtgaaaccat
     2941 gccagaaaaa ccgctgctgt ttaccttccg cagtgccaaa gaaggcggcg agcaggcgat
     3001 ttccaccgag gcttatattg ctctcaatcg tgcagccatc gacagcggcc tggttgatat
     3061 gatcgatctg gagttattta ccggcgatga tcaggttaaa gaaaccgtcg cctacgccca
     3121 cgcgcatgat gtgaaagttg tcatgtccaa ccatgacttc cataaaacgc cggaagccga
     3181 agaaatcatt gcccgtctgc gcaaaatgca gtccttcgac gccgatattc ctaagattgc
     3241 gctgatgccg caaagtacca gcgatgtgct gacgttgctt accgcgaccc tggagatgca
     3301 ggagcagtat gccgatcgtc caatcatcac gatgtcgatg gcaaaaactg gcgtaatttc
     3361 tcgtctggct ggtgaagtat ttggctcggc ggcaactttt ggtgcggtaa aaaaagcctc
     3421 tgcaccaggg caaatctcgg taaatgattt acgcacggta ttaactattt tacaccaggc
     3481 ataagcaata atatttcggc gggaacaccc tccccgccga acttaaaaaa tatatttaac
     3541 cgtatttaat aaaaatattt cgtgagtctc tgtgcgctaa ttctccatct ggcgtaggga
     3601 aaatcacatc tgaatcagga attaacaatg aaacctgtaa aaccacctcg tattaatgga
     3661 cgagtgccgg tcctgtcggc acaggaagcg gtgaattata ttcccgacga agcaacactt
     3721 tgtgtgttag gcgctggcgg cggtattctg gaagccacca cgttaattac agctcttgct
     3781 gataaatata aacagactca aacgccacgt aatttatcga ttattagtcc gacagggctt
     3841 ggcgatcgcg ccgaccgtgg tattagtcct ctggcgcaag aaggtctggt gaaatgggca
     3901 ttatgtggtc actggggaca atcgccgcgt atttctgaac tcgcagaaca aaataaaatt
     3961 attgcttata actatccaca aggtgtactt acacaaacct tacgcgccgc cgcagcccac
     4021 cagcctggta ttattagtga tattggcatc gggacatttg tcgatccacg ccagcaaggc
     4081 ggcaaactga atgaagtcac gaaagaagac ctgattaaac tggtcgagtt tgataacaaa
     4141 gaatatctct attacaaagc gattgcgcca gatatcgcct tcattcg
//
LOCUS       DAZPJR010000248         4133 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPJR010000248 DAZPJR010000000
VERSION     DAZPJR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.254"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            117..530
                     /locus_tag="ACHVB7_004143"
     CDS             117..530
                     /locus_tag="ACHVB7_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR1915971.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(575..892)
                     /gene="hspQ"
                     /locus_tag="ACHVB7_004144"
     CDS             complement(575..892)
                     /gene="hspQ"
                     /locus_tag="ACHVB7_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR1915972.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(950..2140)
                     /gene="rlmI"
                     /locus_tag="ACHVB7_004145"
                     /pseudo
     CDS             complement(950..2140)
                     /gene="rlmI"
                     /locus_tag="ACHVB7_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2235..2513
                     /gene="yccX"
                     /locus_tag="ACHVB7_004146"
     CDS             2235..2513
                     /gene="yccX"
                     /locus_tag="ACHVB7_004146"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR1915973.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2510..2839)
                     /gene="tusE"
                     /locus_tag="ACHVB7_004147"
     CDS             complement(2510..2839)
                     /gene="tusE"
                     /locus_tag="ACHVB7_004147"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR1915974.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2930..3589)
                     /gene="yccA"
                     /locus_tag="ACHVB7_004148"
     CDS             complement(2930..3589)
                     /gene="yccA"
                     /locus_tag="ACHVB7_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR1915975.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3796..3883)
                     /locus_tag="ACHVB7_004149"
     tRNA            complement(3796..3883)
                     /locus_tag="ACHVB7_004149"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3847..3849),aa:Ser,seq:tga)
BASE COUNT         1101 a         1048 c         1017 g          967 t
ORIGIN      
        1 tcatacttac taacatatag acatatttcc cgcttttgct ctcattcatt cgtattagct
       61 gcatggttgg catgtcggct ttgtcgtaca ctttgttaaa cagtctggag gaaataatga
      121 aagaaaccga tattgcaggc attttaacgt ccactcacac cattgcgctg gtgggggcaa
      181 gcgacaaacc cgatcgtccc agctatcgcg tgatgaaata tctactcgac caggggtatc
      241 acgtcattcc ggtttcgcca aaagttgccg gcaaaacgct gctgggccag caggggtatg
      301 gcacgctggc agacgttccg gaaaaggtcg atatggtcga tgttttccgt aattcagaag
      361 cggcgtgggg cgtggcacag gaagctattg ccattggcgc gaaaaccctc tggatgcaac
      421 tgggggtgat taatgagcaa gcggcagtac tggcacggga tgccgggttg aatgtggtga
      481 tggatcgctg cccggctatt gagatccctc gcctgggcct ggccaaataa aaaatccccg
      541 gaaggcaaaa accttccggg gattcgttca gggatcagtt acgcagacgc ggggcctgga
      601 gttgtttgcg gatggtctgc gccagctcat ccatggatgg ctgttcagga tgttcatcct
      661 gcaattcgct acttagctgg gcttcagcca ggtaggtatg aaccggtagg ccgttatcgt
      721 cctccatcac tacgtgatac cacggagcgg cgcgaagctc gtcatttacc gccagctcat
      781 caggcgacgg ttcagagagc gaataaaccg ggtcgatatc cacgaccact ccgagataac
      841 ctaacaggga atggcggacc tgctggccga taccgaattt gctggcaatc atagtcacct
      901 cccgggaaat ctttaccttt gatataaggg taaaattcca catttcaagt tacatgacgc
      961 gacaggcaaa ccctttcaga tatagccctt ccggataggt agcgatcacc ggatgatcgg
     1021 ctgcctgacg gaactgctct ataaattgta catcacggcc ggcatcaatt gcggcatccg
     1081 cgatgatttt ctgaaataaa tcgctggtca tcagaccgga acaggagaaa gtcaggagaa
     1141 taccgccttc attcagcaac tgaatcgcca gcatgttgat atctttatag ccacgacacg
     1201 cgcccatcaa ctggctttta ttctcaacaa acttcggcgg gtccatcacg ataacgtcaa
     1261 atttttcacc gcgatcgcga taagtacgca gcaatttaaa gacatcatca cggacaaact
     1321 cagccttgct cagatccagt ttgttcagct caacgttctg ccgtgcaata tccagcgctt
     1381 cctaggaggt atcaacgctg acaacctggc tgcaaccgcc catcagtgcc gatacggcga
     1441 aaccaccggt ataggagaaa cagttcagca cacgtttatt ttcaacgtag cggcgggtag
     1501 ccaggcggct gtctcgctgg tccaggtagt agcccgtttt gtgtccgtgc tgaatatcca
     1561 ccagcagctt catgccgtgt tcttcaatcg gcagcagggc aggcggcaac tcgccggtga
     1621 cgaggccctg ggtcagctcc atcccttctt ttttacgtac cgcaacatcg ctgcgatcgt
     1681 aaatcgcaca ttccgggtac agcgtttgca gggcactaat taatgccgcg cgctgatatt
     1741 ctgccccagc actcagcagt tgcagcacca gaaaattacc gaaacgatcg atagtaatac
     1801 ccggcaggcc atcagattct ccggcgatta aacgatagct gtcgaggcca tctttttgcg
     1861 ccagtcagtc acgccatttt tgtgcttgtt gcaaacggcg ggaaaaaaaa gcaatgtcga
     1921 tagactcaga cgggtcaaac gtccagacgc gcgcccggat ttgcgaagct ggcgaataag
     1981 cgccgcgtgc taaccatttt ccctgatgat caacaatatc gatggtttca ccgaggctgg
     2041 ctttaccttc catgcgggca acggccccgg aaaagaccca cggatggcga cgaagtaatg
     2101 atttttcgcg ccctttggct aacactaaac gtacactcat aatttacttt tctgtcgatg
     2161 caaagaaatg ggcgtcattg tccggagttt tccatggatt tgcaacgcgt ccattaagga
     2221 taaggagagc agcaatgtcg aaagtctgca taattgcctg ggtttacggg cgagttcagg
     2281 gcgtaggatt tcgctacacc acacagtacg aagcgaaaag actgggttta accgggtacg
     2341 ccaaaaatct tgacgatggc agcgtggaag tggttgcctg cggtgaagaa gggcaggtgg
     2401 aaaaattaat gcagtggtta aagagtggcg gcccgcgttc tgcgcgtgta gaacgggtgc
     2461 tcagcgagcc gcatcatccc tcgggggaat taaccgattt tcgtatccgt taaatacatt
     2521 ttaccggttt tggcaggcca gcaattttgg tggcttgctt agccggacct ttcggaaaca
     2581 gtcggtataa atagcggctg ttaccttttt cttcgccaaa tttattcgcc atcgctttta
     2641 ccagcatacg aatcgccgga gaagtattga attccagata gaaatcacgc acaaaacgca
     2701 ccacttccca gtgttccggt gacagcgaaa tcccttcgtt ctctgcaatc accactgcca
     2761 gcggctcact ccactggctg ctttctttga gatagccttc ggtatccgtt tctatctctt
     2821 taccttcgaa gatcagcata attattacta ccttaatcag actggcggca gtgtaacaaa
     2881 aaacaaagcc ccgcataaag cgaggctatg aaagtgttag cgggtgagat taatcgcggc
     2941 tggcgaagcc cagaatgctc agcaggctga cgaagatgtt gtacagcgaa acatacaggc
     3001 taaccgtggc acgaatatag ttcgtttcac cgccatgaac gatgttgctg gtttcaaaca
     3061 aaatagcgcc agaggagatc agaatgaaga ccgcgctgat cgccagatgc agagcaggca
     3121 gctgcaggaa gatattagca accataccaa tcagcaccac cacaataccc gccatcagca
     3181 taccgccgag gaacgacata tctttgcggg tggtcagcac atatgcagag cagcagaaga
     3241 acactaacgc cgttccgccc agtgccatag cgattacgtc acccattccg gcagacagat
     3301 aggtgttcag aataggtccg aggatataac ccagaaaacc ggtaaaggcg aatgcggaga
     3361 taatcccggt cggcttattc gccgttttat aggtcaggaa catcaaacca tacataccca
     3421 ccagcgtcag aatcagaccc ggagatggca gcatcagcac cgtactggca gttgcggtaa
     3481 tcgccgaaaa ggccagcgtc aggctcagca gaaaataggt attacgcagc accttatggg
     3541 tgctaagcag tgatgtacgg tcatgtgaag aactaacaat acgatccatt gagtcactct
     3601 ctatgacaga tgtaattaat taagcagcat aatgataatg cgtaagggca cccagaagtt
     3661 ttacccatct ttacgcattt gatctggaac aggtttaaca gcggattatc aggtcattaa
     3721 gcaaatataa cgccctgaga atttcgacag gcaaaagaaa aaggggttag catttagcta
     3781 accccttatc ttatttggcg gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc
     3841 cggttttcaa gaccggtgcc ttcaaccgct cggccacact tccggaatga cgcgcactat
     3901 aaacatcccg atgcggcgtg taaaccccta atttgtttgt ttgcctgaaa aacagccaaa
     3961 agtgcattga tagcgtgaaa taacagcaga ttgatcattt catcaccatg aattccttct
     4021 cttttactcg tttagcaacc ggctaaacat ccccaccgcc cggcaaaaag aaaaataggt
     4081 ccatttttat cgctaaaaga taaatccaca cagtttgtat tgttttgtgc aaa
//
LOCUS       DAZPJR010000249         4124 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPJR010000249 DAZPJR010000000
VERSION     DAZPJR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.255"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..83
                     /locus_tag="ACHVB7_004150"
     CDS             <1..83
                     /locus_tag="ACHVB7_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1915976.1"
                     /translation="FWHDLAALVIAGILASMIVNWLNKRK"
     gene            459..566
                     /locus_tag="ACHVB7_004151"
     CDS             459..566
                     /locus_tag="ACHVB7_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1915977.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="ACHVB7_004152"
     CDS             complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="ACHVB7_004152"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR1915978.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="ACHVB7_004153"
     CDS             complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="ACHVB7_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR1915979.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="ACHVB7_004154"
     CDS             complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="ACHVB7_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR1915980.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1051 a         1029 c         1074 g          970 t
ORIGIN      
        1 ccttctggca tgatttagcg gctctggtca ttgctggcat tcttgccagt atgatcgtga
       61 actggctgaa caagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
      121 tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa
      181 cgattgcacg cggataatat tctgcggaat gcgcagcaca gagtgaagac gtgacggatt
      241 ccggaacaga aaaacagtcg tagaatcagc ggcaagccgg aaaggttccg gtgaggcgca
      301 atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
      361 ttgcaggtat gcacctgaca acatcacggg ggctaatctt gaacccaaac aactcaagaa
      421 tagccgcgaa acgttggcat tacaacacag gcggctatat gacgctcgca gaactgggca
      481 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
      541 tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
      601 atttgcgccc ggatcaggcc gcagaggtta aactctgcgg cctttttcgt gcttactgcg
      661 ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcctgg tattgaataa
      721 ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt
      781 tccagtcaac attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc accagatcgg
      841 agatagctag gaagctgctc ggttggtcaa tgacaatcgg tgccccctga tgtggtgcct
      901 tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta gggatatcac
      961 gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga
     1021 ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa
     1081 agaatttctg cgcccgcgct ttgtaatccg ctgttttgct gacccccgga taatggttgc
     1141 cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctgttttta tctttttcgg
     1201 taacgtccag ccagcggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc
     1261 ccagcaaaat aaccggcaga tttgtttgat ccatcaattc agtctgcatg ccgccatttt
     1321 cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc aggtgctggg
     1381 taaagcccag tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc ggttgataca
     1441 gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac
     1501 tgtaggaggt ggcggagtta aagttcttga actcaatatc gaaatgcgac cacagtggat
     1561 gcgacatcaa cccggcggct tctatatccg accaggaaag cgaacagatg ttaatcacca
     1621 gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg
     1681 cctccgcgtt atagaaatta ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag
     1741 tttgtgcggg catatcaccc actaccgatg cgccacccgt cgccgcaacg gttgccgctg
     1801 cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac aaggagaaac
     1861 ttggtcccgc cagggtaagt acgttcagcc atagcagtat ggcaaccaca aaaacggtaa
     1921 tgcgaatcca ttgtgacagg aataaccagg ccactaataa aacaaaaatg gccccaatca
     1981 tctgccagtt aataaagcgt gtgacaaggt cgattaaata atcggtactg aaccccgcca
     2041 cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccaggtgtca tgccagaaca
     2101 aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa
     2161 tgggcatcag cagaaacgtg gcaaacacca aattgaggag cggatggaag ttaagatatc
     2221 ccgcccacaa caggccgaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc
     2281 agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt
     2341 tgccctggct ttttccgtgc ggcgtaacgt cccggccggt ttaacataac gaggtttagc
     2401 aaagaataaa cgtaaggtgt cgcgaatgcg tcgcaaaaag tgccgcgcca gatagcccag
     2461 cgggaaaaat atcagtgcgc aaacgacaat aatttcaatg atatcgctga tggtcatcat
     2521 gatgagcgct ccacagcatc atctaacagt cgcatgggtt ctggtattcg tcgccagtgg
     2581 cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgttaaagg cagcggcatg
     2641 ccccattgtt ctggggcaag caagcgcatc tgcaccagct cggcactgat ttgatcatct
     2701 tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc
     2761 aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg
     2821 ccaatggtca tgatatcgcc ggtacggtta gggcgacaca gcgtcagggc ttgttcaacg
     2881 cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggtaataga
     2941 gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc
     3001 agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggcac atagcgacta
     3061 aacttctgcc cttgcacgct ttcgatcatc gtcagacaac gggagagtgg cgcattccac
     3121 ggaataacca tatttgcacc gcaggccaat aacaaacgtt catcggtggc gcgcaggcta
     3181 gcggtatttt cccgcacgag gattttcatc gcactaccgc gctggcgacg cagggtatga
     3241 atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc
     3301 gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag ttgccagtgt
     3361 tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg tttttcgtcg
     3421 ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg tccagatacc attttgttgc
     3481 tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa ggcaatatcc
     3541 agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga acggtattcc
     3601 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
     3661 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
     3721 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
     3781 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
     3841 aataatttta ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
     3901 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
     3961 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
     4021 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
     4081 tccctcatgt caccattttg tctgattatc agtttactag cgaa
//
LOCUS       DAZPJR010000250         4107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPJR010000250 DAZPJR010000000
VERSION     DAZPJR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.256"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..759)
                     /gene="nlpA"
                     /locus_tag="ACHVB7_004155"
     CDS             complement(<1..759)
                     /gene="nlpA"
                     /locus_tag="ACHVB7_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFR1915981.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
     gene            981..1274
                     /gene="yicS"
                     /locus_tag="ACHVB7_004156"
     CDS             981..1274
                     /gene="yicS"
                     /locus_tag="ACHVB7_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR1915982.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(1315..2495)
                     /gene="nepI"
                     /locus_tag="ACHVB7_004157"
                     /pseudo
     CDS             complement(1315..2495)
                     /gene="nepI"
                     /locus_tag="ACHVB7_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2706..3158)
                     /locus_tag="ACHVB7_004158"
     CDS             complement(2706..3158)
                     /locus_tag="ACHVB7_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR1915983.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(3211..>4107)
                     /gene="adeQ"
                     /locus_tag="ACHVB7_004159"
                     /gene_synonym="yicO"
     CDS             complement(3211..>4107)
                     /gene="adeQ"
                     /locus_tag="ACHVB7_004159"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR1915984.1"
                     /translation="LESLPGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGI
                     LGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSG
                     ALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAG
                     AFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALI
                     FVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRW
                     RDLNLCVVVVASLFALKIILVD"
BASE COUNT         1211 a         1080 c          911 g          905 t
ORIGIN      
        1 tgattgataa gattgcagaa attccttcac gttttctgca ttcttattat cttcccgtgc
       61 caccaaaata ttcacatacg gcgaattctt atcttcaata aatacgctgt cgtgcaccgg
      121 agaaagccct gtctgctgaa tgtaagtggt gctgataatc gctacatcaa ctttgggatc
      181 atccagtacg cgcgggagct gcgccccctc gagttccata atctgcaaat gacgaggatt
      241 atcggttata tccagcgcgg taggtaataa cccttttccc tctttcagag taattagctt
      301 ctctttttgc aacagtaaaa gtgcgcgacc aaggttggtc gggtcgttgg ggatcgccac
      361 tgtcgcgcct tcttttattt gcgccacggt tttgattttt ttggaataac ccgccatagg
      421 gaagacaaac gtatttccca gcgctaccag tttataacca tgcgcctgat tatcttgttc
      481 aagaaagggt cgatgctgga agacattcgc atcaagttcg ccatgatttg tcgcatcgtt
      541 cggtagcaac gaaccgctaa atcctaccag ttcaacatcg aggccatatt tctcttttgc
      601 caccttttta gcgacttccg cgacatcttg ttctgcgcca tttataacgc caactttaat
      661 gtgttttgca tcgctgctac tctggtcgca acctgccagc agaattccgg ccagcaataa
      721 tgcggcccct gtccgtagat gatgtgttgt cagtttcatt tttttatcct tttattaatg
      781 attacctgac tatagcggta aggtcgctgc ggtttaaaaa acgaaacgct atcgataaga
      841 ataaaaagga ataaaagtgg aatataaagt ctggcacttc ttactgacca cgcaagcgcg
      901 tttcgtacag catgatgaga gcgatgaaag caaactacac ttatgcttca ttcgatacac
      961 atttgtaaaa ggttgaatcc atgaaaccaa cgacactact gcttattttc acttttttcg
     1021 ctatgccagg catcgtatac gccgagtcgc cttttagctc actgcagtcc gcaaaagaga
     1081 aaaccacggt attacaagat ttacgtaaaa tttgcacgcc acaggcgtca ttatcagatg
     1141 aagcgtggga aaagttaatg ttgtcagatg aaaacaataa acaacatata cgcgaggcca
     1201 tcgtggcgat ggaacgtaat aatcagagca attattggga agcgttgggt aaggtagaat
     1261 gcccggatat gtaagaacag caacaactac tgaccaacgc ttctttacct gatatcagga
     1321 tttcttcatt ttcacctttg cagtaaccaa caatgcagtc agcaacatca atgtgccaga
     1381 aaacatcaac ggcgaagtca gaccaatatt atccagcgca taaccgccga ttgccgcgcc
     1441 acaagctgaa taaccgccac ctgaatagac ccggcttttt ctgcctgatc ggccagtgag
     1501 cgggtgatcc acgttgacca gccgacggga accaatgcaa aagttagccc ccagataatc
     1561 gccacgccgg tagcaacgat tttatcgctt ccccacaacg tcagcaccaa cgcactcaca
     1621 gccagtatta acggcgcgcc tgctaaggcc agttttaccg aacgtttaag aatgaatgac
     1681 gaaagcgacg taccgataaa gctggcaatg ccaaaactca acagcaccag cgttaagcca
     1741 tccacgctga atcccgccag gttcatatac accgggcgaa tatatgtgaa gaaagcaaac
     1801 tgcccggcga aagacatgaa gatggcgatc atccctgcca tcacacccgg acgttgtaat
     1861 aagcggaaag tattttgttt ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg
     1921 ataatccaga aaatacacag cacgcccatc accgccgccg cattaaagac attgcgccaa
     1981 ccgataagct cgcctaaaaa actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc
     2041 gcgccgaaga tcaccgacag cgccttcggc accgtacgcg gcggcaccag acgcatggtc
     2101 agcgacgccg acatcgccca gaacccgccc agcgccagcc ccagacaggc acgaccgatt
     2161 aaaagcaaac tgaatgagtt agcaaaggaa accagcaagc aggagagcgt cagcaaaacg
     2221 gcaaacaaaa taacaacgtt gcggcggtca gtagcctgaa ttgtctgggt aataaacaaa
     2281 ctggcaaaca ttgccacaaa ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa
     2341 atgcctaaat cctgggccat tggcgtcaac aaactgacgg gcaaaaactc aacgataatc
     2401 agacaggcga cacaaaacgc caccgagaaa acggctgacc agttcggtcg ggtgatggca
     2461 tccgcgccgc ggttttcggc aataaattca ctcatggtgt tacccgtggc atgtttcagg
     2521 ggaaaagccg accactttaa cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt
     2581 acggtgttgc ttgagctgga gttaagatcg aaacggaaca gggcaaagat taccggatgc
     2641 ggcgtgaacg tcttatccac ccgacaggtc aggcgcggtc tgtaggctga taagacgaag
     2701 cggttttaat gcagccagtg aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc
     2761 tgaaaagcat taatatcctg catttcaggt tcggtttcgc gcagaaaacc cagcgccagc
     2821 ctgacctgag catcggtaat cggcgcggaa aggcgggctt tttgtaaaat ccgatgccat
     2881 tgcagcaggt tctgttcgct accatcgaca atgacaattt gccagatgag caacacctgc
     2941 gcggcgtttt gcaggtgcga ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg
     3001 gctgactttc ccatttgatc gaccatcgac tccacgggta cgacatcgat attcagccga
     3061 tcactgagac ggcgaatcgc ttttctggcc ccggatgtca gcacccgaaa accaaccaca
     3121 aacactaccg ccagcgtggc gagcattatc cagatcatga gttctcctgc gttatcagaa
     3181 ctcatgatat ctggaaaccc tcgccagtta ctaatccacc agaataatct tcagtgcaaa
     3241 cagagatgcg accaccacca cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt
     3301 catgatgcag tacgacataa agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg
     3361 catcatcacc gtggtaataa acgcaggcac cgattcggta aaatcatccc agttaacgcg
     3421 cgccaggctc gaagtcatca gcacgccaac aaagattaac gctccggcgg ttgcgtaagg
     3481 aggaaccatc gccaccagcg gggagaagaa cataaccaac aggaacataa cgccaaccac
     3541 aaccgcagtc agccccgtgc ggccaccgac tgccacacca gaagtacttt caatataggc
     3601 agtaacagac gaggtgccga taaacgcacc cgccaccgaa ctgacgctat caacatacag
     3661 cgccttattc atattgggga atttaccgtt accatctatt aagcccgctt tatcagttac
     3721 accaattaat gttcctgatg aatcaaatag gttgatcagc ataaaggaga aaatgatacc
     3781 ggcgagttca agtgttaacg cgccgctcaa atctacttca ccaatgacgc cgctaatatc
     3841 aggcggaatg gaatagacgc cgctaaaatg aacatcaccg aaaaataatc cacagcaaga
     3901 cgtcaccaca atagaaacca gcaccgcggc atgaaaatga cgtgatgaca acacggttat
     3961 aataaaaaaa cctaaaatac ctaacaacac gccgtgagaa cttaaatcgc caatcatcac
     4021 cagcgtgtct ttattggcga caataacgcc agtatttttt aatcccatta aggcgataaa
     4081 taatccaatt ccgggtaatg actccaa
//
LOCUS       DAZPJR010000251         4085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPJR010000251 DAZPJR010000000
VERSION     DAZPJR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.257"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            44..568
                     /gene="radC"
                     /locus_tag="ACHVB7_004160"
     CDS             44..568
                     /gene="radC"
                     /locus_tag="ACHVB7_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR1915985.1"
                     /translation="MNKLESLPGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYN
                     VRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEV
                     HPREIIREAIKINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVI
                     GRGEYVSFAERGWI"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="ACHVB7_004161"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="ACHVB7_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR1915986.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHVB7_004162"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHVB7_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR1915987.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="ACHVB7_004163"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="ACHVB7_004163"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR1915988.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHVB7_004164"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHVB7_004164"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR1915989.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHVB7_004165"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHVB7_004165"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR1915990.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
BASE COUNT         1011 a         1003 c         1084 g          987 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgt
//
LOCUS       DAZPJR010000252         4082 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPJR010000252 DAZPJR010000000
VERSION     DAZPJR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.258"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(46..747)
                     /locus_tag="ACHVB7_004166"
     CDS             complement(46..747)
                     /locus_tag="ACHVB7_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1915991.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPK
                     LQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYAKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTG
                     ERIIALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1280..1534)
                     /gene="mobC"
                     /locus_tag="ACHVB7_004167"
     CDS             complement(1280..1534)
                     /gene="mobC"
                     /locus_tag="ACHVB7_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR1915992.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
     gene            1713..3872
                     /gene="mobQ"
                     /locus_tag="ACHVB7_004168"
     CDS             1713..3872
                     /gene="mobQ"
                     /locus_tag="ACHVB7_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR1915993.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEAL
                     PKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGL
                     AALRVQLLKREEELRRKEQEDTERSRTERRAEQLRKNDNAVMDSGVLSAVVTGYGEAP
                     MPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHP
                     PLQRVYSSATFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVD
                     FTQGRLLPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNS
                     QLVISALTDKGLRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQP
                     DHLFRSLGFNRTWDEWRREEDARTHATERRHDRGWSQ"
BASE COUNT         1075 a         1032 c          985 g          990 t
ORIGIN      
        1 aaaaaaaacg cagcagggag cgttttaggt gccttaaaat cgattttaga tcgtcatctt
       61 gatttgtttg tcttccttga agcgaaaatg cagggctaca accgtgcgcc cctttttgat
      121 cgtctcaacc gttacagcca ggtcgctttt tttgttcagt tcgtcaatgc agggtcttaa
      181 taattgctgg ttaagtgatt tatatgtttg atatttatgc tctatatcaa gcattgagcg
      241 aaaatcatcg agagcgataa tacgttcgcc agtactgcgg aactgctgga ggagttcgta
      301 tatccttata ctgtaacttc ttgataatct agatatattc ttgattacaa ctctagtaaa
      361 ttgcccttgt agttgtgtca aataaggcat tactgcgtca gaaaatgtta tttgtgctct
      421 ggcctcgcct ttagcgtact gtgcccgata ctggatccag cggaactcct cacgtttctc
      481 gtcatccttg agaatgattg acctgtccca caaacgatca attgcttcct gtaactgaac
      541 ctcggcattt ttccgcccca tgtcagggaa tgaatcgaaa tattccgcag ctgtaatggt
      601 cattgtcttt tgcaattttg gagattctgc atcagcccca gacttcaatc gaccgatgca
      661 aagtaacaaa aatcgctgtt cctgtagggt caatttataa ctggcttcaa ccagcgcatt
      721 ggatttatag gcaattagat ccgacataga gcatccctcc ccaaaaggta atgaataaaa
      781 gataaacata ccctcaacga aaggcaatgc ctttttattt taatatccct ttttctgtga
      841 atattacctt aaatcatatt gcctttacct taaattcccc cacctaaaca ccttaaatca
      901 tattaccttt accttaaatc acattaccta tagcattgta acattttgtt ttctataagg
      961 aattttggcg ctaaaacaag tattaaaaac aagatcttaa aaacaagatc taaaaactta
     1021 acgacgagcc tgtggataac ttggagtgct gaaaacaaaa aaccaataaa aacaatgcgt
     1081 tgcaaaaaag cagaattgca agttccgaat acaccccctc caaccctgtg gaaatccagt
     1141 attcatgcgg gttcagcgca tatcacctcc cgaaaaaccg tgcgtatgtg ctcaatcagg
     1201 cgacggattc acttccgctt cctcgcccac tcgctcgcta acgctcggtc gctgggactg
     1261 cggcgagcgg acgatacaat cactgtcctg gcagcgcttc cagctcgtta atcagcggca
     1321 gtatgcgcgt cacatcagcc tgtcgtgtca cctctgcctt caggaactgg agtaaccagg
     1381 cacgcaactg cacctcctcc ggctttctga cctttgccag caatgctgca cccacaatga
     1441 ccttctgcgc tgtgtccaga ctcctgctct tcgccttcag gcgctgtaat ctggcctcag
     1501 catcagcaat cttctgttcg agtgttctgc tcatgatgta actccgtgcg cgatgaaaaa
     1561 tcgcatttta gcgcgtcact ggtagtttaa aaactgagct ggcataatgc acggaaaatc
     1621 acgaagtgcg cacttataca atctccactc cgtttcgatt gtgtgcgccc tgcggggctg
     1681 aaagaaaacc acaaaaaggc attacggcag aaatggcgat ttatcatctc agcatgaaaa
     1741 tcatctccag aaacagcggc tacagcgccg ttgcttctgc tgcctatcgt tccggttcac
     1801 tgatgcttga tgagcgtacc ggactgactc acgactacac ccgcaaaagc ggtgtggctg
     1861 aagctgtgat tctgactccc gcgacagccc ctgcctggtg tacgaaccgt gccgaactct
     1921 ggaatgccgt tgagaaggcc gaacgccgga agaactcaca acttgcgcgg gaaattgaac
     1981 tcgccatccc ccgcgaacta ccgcaggatg ccgcacggga aaccgttctc gcctttgtca
     2041 gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc gttccatcac atggataaaa
     2101 caaatcccca tgcgcacatc atgctgacca cgagagctgt cggacctgct ggattcggtg
     2161 gaaaggtcag ggactggaac gaccggacac atgccgagac gtggcgtgca tcatgggctg
     2221 accatgcgaa cagggcactg gcgaacgccg gttatcagga agagatagac catcgttcat
     2281 acgagcgaca ggggcttgag aaaacgcctg gtattcacct cggaaagtct gcctgtgcga
     2341 tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt aacagactta
     2401 acctggaaat acagatttcc cgtaccgaac tcagaaacag cgggctgaca gagcctgccc
     2461 gccgtgtggc tgaccttctg aatatcgtgc ttcctgacaa cgcaacttcg tacacactac
     2521 gtgacatcat cgaagcgttg ccgaaggaca gtaatgcggc atggaaactc accagtaatg
     2581 ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagag ctgaacaccg
     2641 aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg ttttcgtccg
     2701 gtttttcccg tattcccctg atgcaatggg cggcaccgga ataccgtagc gaacagggaa
     2761 aaatccagtc gctgagccag caaatggaaa aattgcgaca gcattaccgg aaggtggaga
     2821 aacaggacat tcccgcgagt cagacggcat ttgaaaaaca gtggcagcag tggggggcat
     2881 ccggccttgc agctctcaga gtccagttgc ttaaacgtga agaggaattg cgccggaagg
     2941 agcaggagga tacagaacgt agtcgcacgg agcgacgagc ggaacagctc aggaaaaatg
     3001 acaatgccgt tatggattca ggggtactga gcgcagtcgt tacggggtat ggcgaggccc
     3061 ccatgcctgg taacggggaa atgacctgtt accttctgct tcacaaccgg aacggagaat
     3121 acacgctctg ggggaacgag ctggagaaat accgcaccag aatctttgag cgtgttgatt
     3181 taatgcgtga caggagtggt tatatctgtg accgaagcga aatcggtcaa catccaccac
     3241 tgcaacgtgt ttattcctct gcgacctttg agcaacttct gacgcaggtt tgccagacat
     3301 ggccgcaata tacccgcaat ctccgccagc cgaagacatg gccggagtct ttctgtcttg
     3361 gagaagaccg ccagccagcc atgccatcac tggcagcaag gaaggtggat tttacgcagg
     3421 gacgtctgct accgacgctg atgccggtaa tgagttctgt cgaccgggaa acccgccagc
     3481 ttcagctttt gctggtcatg ggcgtggatg actctctggg cggtgttgtc cggcttaatg
     3541 gtacgctgta tcccgcgttt gccgttccct cagccgataa cagccagctg gtcatcagtg
     3601 cactgaccga taaggggtta cggtatgcag ggtatggtgt tgccgtaaat cacgatgcag
     3661 acagccacat atcacctgcg ccggaactta tggagtttca tctgaaaacc cgtgaggcac
     3721 cgctttttgc tgccgtcaac acaccagaaa aacagccgga tcatctgttc cgctcgctgg
     3781 gttttaatcg tacctgggac gaatggcggc gcgaggaaga tgcacgaaca cacgccaccg
     3841 agaggagaca tgatcgcggt tggtcacagt gaatgtgaac aatattttgt tttgtttaac
     3901 atcgtgttat gttcgtcgat gttcttcgat gttcatcgac agggatgaat gcaagaagca
     3961 cacacataat gcaagcatag ttttgttttt ccgcccccaa aaaatggggg ttcttttttc
     4021 gctgcattag agcgcttttg acgcaatttt tcacttcaat agtaccttcg cactactaac
     4081 ac
//
LOCUS       DAZPJR010000253         4079 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPJR010000253 DAZPJR010000000
VERSION     DAZPJR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.259"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..787
                     /locus_tag="ACHVB7_004169"
     CDS             <1..787
                     /locus_tag="ACHVB7_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1915994.1"
                     /translation="IAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            800..1760
                     /locus_tag="ACHVB7_004170"
                     /pseudo
     CDS             800..1760
                     /locus_tag="ACHVB7_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1798..2697)
                     /gene="nhaR"
                     /locus_tag="ACHVB7_004171"
     CDS             complement(1798..2697)
                     /gene="nhaR"
                     /locus_tag="ACHVB7_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR1915995.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2763..3929)
                     /gene="nhaA"
                     /locus_tag="ACHVB7_004172"
     CDS             complement(2763..3929)
                     /gene="nhaA"
                     /locus_tag="ACHVB7_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR1915996.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1226 a          953 c          861 g         1039 t
ORIGIN      
        1 tattgccaaa acacgtcacc aaattaactt atcgaattcg acctcatttt ccaaagatgg
       61 ctattcctcc aacaatactg gaattactgg catagccggt gaacatgatc agttaaatta
      121 tggtatatat gttaatcagc aacaacaaaa taatgatacc tcgcttggta cgaatttaag
      181 ctggagaact cccatcgcca taatagatgg cagctatagt cattctaaaa acgcctggca
      241 aagtggtgga agtattagta gtggattagt tgtctggccc ggtggtatta atatcactaa
      301 ccagttatcc gatacatttg caattctgga tgcgcctggg ttagaaggtg cacatattaa
      361 tggacaaaaa tacaatcgaa caaacagcaa aggccaggtt gtttacgacc tgataatacc
      421 tcatcgtgaa aaccatctgg tacttgatat agcaaacagt gaaagtgaaa cagaattgca
      481 gggcaatcgt caaattattg caccttaccg tggagcagtt tcttatgtgc agtttacaac
      541 tgaccaacgt aagccctggt atatacaggc actgcgtccc gatggttcgc cattaacctt
      601 tggctatgac gtactggatc tccaggaaaa taatattgga gtcgttggcc agggtagtcg
      661 cctttttatt cgcgtagatg aaattcccac tggaataaaa gttgctctca atgatgaaca
      721 gaacttattc tgtactatta cttttcaaca cgttatcgat gaaaacaaaa catatatatg
      781 ccagtaaaag gagtttacta tgaagtggct tttattaatc accttgagtc tatattcttt
      841 tattgtgcaa agtgctccct gtgcattgac caatgtcggg gaacaacgtg gaacatatat
      901 actcaagcca ctgagtatga aaggtaatct caccgccgaa aaaattgaaa acaccggaaa
      961 tatcattgag tgtgcagact tacaagagac gaccaacgcc attaaagttc attttttaac
     1021 acagaacgct ttaaggttta aagtcggaag tcgtcattat attattaatt tcccctttga
     1081 atctgacggt aaaaaaaata gagcttccag gaaggtcatc ttataattta gaagaaatgc
     1141 tatctgcgat aaatttcacc attccatact ctatcgcatc tgtatcagat agttccaaca
     1201 ccgcagatat ttttccagga actccttttc ctttgttgtt cgaaattgaa gtgagttcat
     1261 gtacagggaa taatactaat tgtaatagcg tccgctttaa ttataattta aatactattt
     1321 tacaggtaaa tttaatgacc tgtggttttg aagatcaatc catcgacact ggacgatttg
     1381 tactgtctcg tatcaataat gaaaattatc agtttcatca tataagattt gactgcatcc
     1441 agggcgagca aggtgaatta gtttttgaac ccagtgatgt tgaatattac tttgaaccag
     1501 tgacgataca ggagtcagga accagcatat taaaaaatga gttagaaaac agtgaggacg
     1561 gtgcgggtca agtaggcttt caaccctcca atgatggtac gaatgaaatc cagtatggta
     1621 aaagcaatta ctactctttt cagcatcccc atgaaggaag taatcagatt cctctattta
     1681 ttcgcccgcg tacatatgga aataatgtct caagcgggca aataatgagc cgagtcaaaa
     1741 tcgtggttat gtacaactaa tctatctccc ccaccttaac tttgggggag gtgccgatta
     1801 acgcgccgct ggactaaaaa gcgcagaata atccgtattg cagattcgct gtaccgccgg
     1861 gtgctgaatc atccgctcag caaaaatagc atggtactct tccatcacat tctcgacgcg
     1921 accaatttct acgacagttt tatcggcata aaagtcatat gcataaagcg ttggggcaac
     1981 gaagattgca ttgtgcatcg caccaaaagc tttcatcaac gcggcatcat caaactcgcc
     2041 gaggatttct acgtttaatc cctgggagtt aaaccagtta agcaatttgc gccctaacat
     2101 tgaacgtcgc ccaggaatca aaagtcgccg ttcttccaga caagccggga acgatttttc
     2161 tggtggtgga tttgtacacc agaaactcac gccacattcg ccaattctca cggagaacag
     2221 gccttcctgc tgcgtagagt ctatcggaca gtcagaaatg atcatatcca gtttatgctg
     2281 acttaattgc tccagcagca tttcgtgggt ggattcgaag cagcgaagat gaatgggctc
     2341 gccttctacc actgcggcgt taagtacgct actgaccagg cgtttggaaa gtgcatcagc
     2401 cacgccaacg tcaaacaata aattggattc tttgcgatag ttcacaatat ccagcatttc
     2461 ctggcttaag gtgaacattt tatcggcata gcgatagacc agttctccca gctcgctggg
     2521 ttcgagacca cgtcccttgc gtttaaataa tttgccttgc aggcgctctt ccagcgctcg
     2581 aatctgtccg gtaatggttt gtggagttaa ataaagcgcc tccgctgcgc caaccacgga
     2641 accttcttta tagacatgcc agaagtaata caagtggttg taattgatat gagacatgct
     2701 catttctctc cctgataaca atgaaaaggg agccgtttat ggctccccgg taaaccgtcc
     2761 tgtcaaactg atggacgcaa acgaacgcgt aaccagctgt atccaattac cgccgaagag
     2821 atagaaccga ccaggatacc gagtttcgcc cagttaatca gttctggatc tacgctacca
     2881 aaggccaggc tggcaataaa gatagacata gtaaaaccga taccgcacag gatccccacc
     2941 gccataattt gctgataagt cgttccctca ggcagatgcg ccagtttcaa acgcagcgcc
     3001 aaccagcaga acagactaat ccccagcggt ttgccaatca gcaagccagc gatgatcccc
     3061 aatggcagaa tggaggtcaa gccatccagc gtgacgcctt gcagtgaaac gccagcatta
     3121 gcaaatgcaa acagcggtaa aatcagatac gccacccacg ggtgcaatac atgctccagt
     3181 cgcttcgctg gagaacgccc atgcttctct ttcaaaggaa taaagaagcc gacaattacc
     3241 cccgccaggg ttgcgtgaac ccccgatttc aacaccgcag tccacaacac cacgccaaca
     3301 agaatataga cgcccgtgcg gcgtacacca cacagattca ataccgcgag taccgcaatt
     3361 gctacagccg cgacgccaag agaggccatc gataagtcat tagtgtagaa caatgcgatg
     3421 ataatgatgg ccccaagatc gtcgataata gccagagcca tcaaaaagat cttcagtgct
     3481 aacggaacac gacttcccaa cagcgccagc acaccaagtg caaaggcaat gtcagtagcc
     3541 gccgggatcg cccacccttc gcgggtaatc ggatcggcat agttaaaggc cagatagagt
     3601 aatgccggaa caatcatccc accaatagcg gcgataaccg gaaatgcggc ctggcgtaag
     3661 ctggctagcg atccttgcat cagttcacgt ttaacttcca gaccgactaa caggaaaaat
     3721 accgccatca gcgcgtcatt tatccataac agcatgtttt tgttgatttc gagtgaacca
     3781 acccggagct gaaccggcgt ctccagaaag tcgtgatacc atccactggt tgcgccgctg
     3841 ttggccatca tcatcgccag gatagcggca atgataagaa taatgcctcc cgaggcatca
     3901 ctgctaaaga atcgatgcag atgtttcact ttttatttct ctttcaggtg aatagatcgt
     3961 ttttactatt ttaccccgca caaatcatcg ttaaaatcag attatagtgt aggaataact
     4021 cgttttttac gagcaattaa aggatttgcg ccatcagcgg tgagatgagt ttttgacgg
//
LOCUS       DAZPJR010000254         4048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPJR010000254 DAZPJR010000000
VERSION     DAZPJR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.260"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHVB7_004173"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHVB7_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR1915997.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHVB7_004174"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHVB7_004174"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR1915998.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHVB7_004175"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHVB7_004175"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR1915999.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHVB7_004176"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHVB7_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR1916000.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          970 a         1143 c          971 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacccga
//
LOCUS       DAZPJR010000255         3983 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPJR010000255 DAZPJR010000000
VERSION     DAZPJR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.261"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(1..2602)
                     /gene="acrF"
                     /locus_tag="ACHVB7_004177"
                     /pseudo
     CDS             complement(1..2602)
                     /gene="acrF"
                     /locus_tag="ACHVB7_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2614..3771)
                     /gene="acrE"
                     /locus_tag="ACHVB7_004178"
     CDS             complement(2614..3771)
                     /gene="acrE"
                     /locus_tag="ACHVB7_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR1916001.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT          973 a         1043 c          985 g          982 t
ORIGIN      
        1 ttcctgatac gacatacctg tccagtcata accaatgccc gcaggtaatt ttgacgcaag
       61 gttttccatc aatgccatgg catcgccgga actggttcct ggcgcggctt ccccctgaat
      121 ctccattgac ggcagaccgt tgtagcgttc cagtcgcgga gagccataca cccaatgtga
      181 agtggtaaag gcagagaatg gcaccatttc gccgttggcg ctgcggacat aaagtttatc
      241 gacatcttct ggcagcatac ggaatttggc atccgcctga acatacacct ttttcacgcg
      301 accacggtcg atgaagtcgt taacgtaagt cccacccagc gccgttgaaa tggtctgatt
      361 gatgtcagaa agtgaaacac ctaatgcctg cgccttttcc tggtcaactt ccagtttaaa
      421 ctgcgcggtg tcttccagac cattaggacg cacgctgact aagctggcag gatgttgcgc
      481 cgccatgcca agcaactggt tacgggcctg ggtcagggca tcgtgaccca gcccagcctg
      541 atcaattaac tcaaagtcga aacccgttgc cgtgcccagt tcaacaatgg ctggcatatt
      601 gaatggaatg acaaaaccgt cgcggatctt gcccaattcc attttggcac gatggattac
      661 cgcttccgca ctgttttcgt caccactacg ctcttcccac ggtttcagac tgacgaaagc
      721 cataccggcg ttttgtgcct ggccgctgaa gctaaagccg ttaaccgtaa agacactttc
      781 tacgttcgct ttctcgttat tcagatagta atccgtcact tgatccaaca ctttttgcgt
      841 ccgctcttgc gtcgcgccag cgggtaactg aatcatggtc agaaagacac cctgatcctc
      901 ttcaggtaag aaggaagaag gaagacggaa gacgtaaaaa caacaccacc attcctgcaa
      961 caatcagcgc atagatcagt aaatatcgtc ctgtggaacc gaggattttg ccgacgctgt
     1021 tggtgtagtg gttaacgcta tgatcgaagg tggtattaaa ccagccgaag aaaccgccct
     1081 tattttcgtg atgctcagca gagacgggtt taagcagcgt tgcacataac gcaggggtaa
     1141 gaatcaatgc caccagaaca gaaagcgcca ttgccgaaac gatggtgata gagaactggc
     1201 gataaattgc cccagtagaa ccgccgaaga atgccatcgg aataaatacc gctgacagca
     1261 ccatcgcgat acccaccagt gcgccctgaa tttgcgacat cgatttttcc gtcgcttctt
     1321 ttggcgggag cttatcctcc atcatcacgc gctcgacgtt ctccaccacc actatcgcat
     1381 catcgacgag cagcccgatg gcaagcacca tcccgaacat cgttagtgtg ttgatggagt
     1441 aaccaaaagc ggcgaggatg gcaaacgtcc ctaacaacac cacgggtacc gcaatggtgg
     1501 ggatcagcgt tgctcgcata ttctgcaaga acagatacat caccaggaac accagcataa
     1561 tggcttcgaa cagcgttttt accacttcgt gaatagaaag ctggacgaat ggcgtggtgt
     1621 cataagggta gagaaccttc attccctgcg ggaagaatgg ctgtaattcc gccagttttg
     1681 ccttaatggc tttcgcggta ttgagagcgt tcgcgccggt tgctagctta atccccaggc
     1741 ccgccgccgg ttttccgttg atacgagcga taacgttata gttttcaccg ccaagttcaa
     1801 ctcgtgcgac atctttcagg cgtaccaccg agccgtcact gtttacgcgc agggttactt
     1861 tgccgaattc ttccggattt tttaaccgcg tctgagcaat aatcgaggcg ttcaactgtt
     1921 gccctggtaa cgctggcgtt ccgcccaact gcccggcagc gatctgatcg ttctgtacct
     1981 tcaactggtt aatcacatca accggtgtca gtttatattt gtttagcaga tcggcgtcca
     2041 gccagatacg catcgcatac tgtgcgccga aaagctgtac gtcaccgacg ccattcagac
     2101 ggctaagcgt atctttaacg ttagaggcca catagtccga gatatcgtcc tgtgtggtgt
     2161 ctgggttatc agagacaaag cccgccacca tcaaatagct gctactggac ttttcaacac
     2221 tgatcccctg ctgctgaacc tcctgcggca gcaacggcgt ggcgagctgg agtttgttct
     2281 gcacctgcac ttgcgcgata tcaggatcgg tcccggactg gaaggtaagg gtaattgtca
     2341 cgctaccggc ggaatcgctg gtggaggaca tatacatcag gttatcgata ccattcatat
     2401 tctgttcgat aacctgcgtc accgtatcct gcacggtctg cgcatcagca cctggatagt
     2461 ttgcctaaac agaaaccgca ggcggtgcaa tggttggata ctgagcgacg ggcaattgta
     2521 ggatcgccag tgcgcccgcc atcatcagaa taatggccag cacccatgca aatatcggtc
     2581 gtcgaataaa aaagtttgcc atgtcagatt accttacttc gatgcagtat ctgcgggggt
     2641 atcggtagtg gctttaacct gctctcccgg acgcgctttt tgcaggccgc tgacaatgac
     2701 ttgatcgcca gatttcagac cttcgctaat caaccattta tcgccaatcg cctgactggc
     2761 aacgacaggg cgcgcttcca cctgactttt atcgttaaca atcagcacgg ttgcatcacc
     2821 acgcggtgtg cggctaacgc cttgttgcgg gataagaatg gcgtcaggtt ggacgccttc
     2881 atcaatccgt gcacgcacaa acatacccgg caaaagcgta tgttgcgggt tagggaagac
     2941 agcacgtagg gttatggagc cggtgcattc atcaacggtc acatcagaga attgcagcgt
     3001 acctttcagg ggataggttt gaccgttttc catgaccaac tctacgttgc tggtggcgtt
     3061 ttccttatgc aaatttcctt gctctacgga ttgcttcagc ctcataaaat cgttgctgga
     3121 ttgggtcaca tcaacgtaga taggatcgag ctgctggaca gtcgccagtt cagtcgtttg
     3181 cccattagtg acaagagcgc cttcggtcac ggtcgatttg ccgatacgtc cgctaattgg
     3241 cgcagtcact ttggtataag cgagattgat gcgagcgctt tcgactgtgg cttttgcggc
     3301 aatcacggcg gcatcggcct ggcgagcatc agcaatggcc tggtcgtact cctgctggct
     3361 gatgtatttc gtacccacga gcggaacgta acgttttacc gtcaaatgcg cgatggcggc
     3421 ggcggcttca cttttcgcca gttcgccttt cgcgctgtca taatttgcct gataggtcgc
     3481 gggatcgatc tggtacaggg actggcctgc ttgtacatcg ctgccttcag tgaaattacg
     3541 attcagtacg atcccgctaa cctgtgggcg aacttcggct atacggtaag cattggtgcg
     3601 gcctggtaat tcagtcttaa cttctaacgg ggccgttttc acaatatgaa cggtaacctg
     3661 cggttcaccg acgtgagctt tctcttctcc cttatcgtta caaccgacga ttaaagccgc
     3721 ggagatcaga ataaaggagg gcaggaggaa aaacctggca tgtttcgtca ttactattcc
     3781 tcaaaaaaac aaaagcgcgt tatttaccca gaaaggcaac gcgttaactc gcagaaagaa
     3841 aaatacagtt tgctatccta caaattatca ttcgtcgatg taaggaatag ttatgaatac
     3901 tggcgtctca aggcacataa acacaaaaaa agattaatat tctactgttt tattttgacg
     3961 cgggttgaaa gaggcagaat taa
//
LOCUS       DAZPJR010000256         3975 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPJR010000256 DAZPJR010000000
VERSION     DAZPJR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.262"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..493
                     /gene="tolA"
                     /locus_tag="ACHVB7_004179"
     CDS             <1..493
                     /gene="tolA"
                     /locus_tag="ACHVB7_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR1916002.1"
                     /translation="AAAAKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELS
                     SGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAG
                     KTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLD
                     FKP"
     gene            626..1918
                     /gene="tolB"
                     /locus_tag="ACHVB7_004180"
     CDS             626..1918
                     /gene="tolB"
                     /locus_tag="ACHVB7_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR1916003.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            1953..2474
                     /gene="pal"
                     /locus_tag="ACHVB7_004181"
     CDS             1953..2474
                     /gene="pal"
                     /locus_tag="ACHVB7_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR1916004.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            2484..3275
                     /gene="cpoB"
                     /locus_tag="ACHVB7_004182"
     CDS             2484..3275
                     /gene="cpoB"
                     /locus_tag="ACHVB7_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR1916005.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            3440..3515
                     /locus_tag="ACHVB7_004183"
     tRNA            3440..3515
                     /locus_tag="ACHVB7_004183"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3473..3475,aa:Lys,seq:ttt)
     gene            3665..3740
                     /locus_tag="ACHVB7_004184"
     tRNA            3665..3740
                     /locus_tag="ACHVB7_004184"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3698..3700,aa:Val,seq:tac)
     gene            3744..3819
                     /locus_tag="ACHVB7_004185"
     tRNA            3744..3819
                     /locus_tag="ACHVB7_004185"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3777..3779,aa:Lys,seq:ttt)
     gene            3868..3943
                     /locus_tag="ACHVB7_004186"
     tRNA            3868..3943
                     /locus_tag="ACHVB7_004186"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3901..3903,aa:Lys,seq:ttt)
BASE COUNT         1033 a          969 c         1054 g          919 t
ORIGIN      
        1 agcggcagcg gcaaaagcag cggcagaaaa agccgccgca gacaaaaaag cggcagcggc
       61 aaaagcagca gctgaaaaag ccgctgcagc aaaagctgcc gcagaggcag atgatatttt
      121 cggtgagcta agctctggta agaatgcacc gaaaacgggg ggaggggcga aagggaacaa
      181 tgcttcgtct gccgggagtg gtaatactaa aaacaatggc gcatcagggg ccgatatcaa
      241 taactatgcc gggcagatta aatctgctat cgaaagtaag ttctatgacg catcgtccta
      301 tgcaggcaaa acctgtacgc tgcgcataaa actggcaccc gatggtatgt tactggatat
      361 caaacctgaa ggtggcgatc ccgcactttg tcaggctgcg ttggcagcag ctaaacttgc
      421 gaagatcccg aaaccaccaa gccaggcagt atatgaagtg ttcaaaaacg cgccattgga
      481 cttcaaaccg taatcgcgat gttgactgtt ccgacggtca acatcaggca ccggttgcca
      541 cggggttctg gtagttttgt gtattttagt ttgttaacat tctgctaaat tatcgtgggc
      601 catcggtcca gataagggag atatgatgaa gcaggcatta cgagtagcat ttggttttct
      661 catactgtgg gcatcagttc tgcatgctga agtccgcatt gtgatcgaca gcggtgtaga
      721 ttccggtcgt cctattggtg ttgttccttt ccagtgggcg ggccctggtg cggcacctga
      781 agatattggc ggcatcgttg ctgccgactt gcgtaacagc ggtaaattta atccgttaga
      841 tcgcgctcgt ctgccacagc agccgggtag tgcgcaggaa gtacaaccag ctgcatggtc
      901 cgcgctgggt attgacgctg tagttgtcgg tcaggtcact ccgaatccgg atggttctta
      961 caatgttgct tatcaacttg ttgacactgg cggcgcaccg ggtactgtac ttgctcagaa
     1021 ctcgtacaaa gtgaacaagc agtggctgcg ctatgctggt cataccgcca gtgatgaagt
     1081 gtttgaaaaa ctgaccggca ttaaaggtgc gttccgtacc cgtattgcct acgttgttca
     1141 gaccaacggc ggtcagttcc cgtatgaact gcgcgtatct gactatgacg gttacaacca
     1201 gtttgtcgtt caccgttcac cacagccgct gatgtctccg gcgtggtcac cagacggttc
     1261 taaactggct tatgtgacct tcgaaagcgg tcgttccgcg ctagttatcc aaacgctggc
     1321 aaacggcgct gtacgtcagg tggcttcatt cccgcgtcac aacggtgcgc ctgcattctc
     1381 gccagacggc agcaaactgg cattcgcctt gtcgaaaacc ggtagcctga acctgtacgt
     1441 aatggatttg gcttctggtc agatccgcca ggtgactgat ggtcgcagta acaataccga
     1501 accgacctgg ttcccggaca gccagaacct ggcatttact tctgaccagg ccggtcgtcc
     1561 acaggtttat aaagtgaata tcaacggcgg tgcgccacaa cgtattacct gggaaggttc
     1621 gcagaaccag gatgcggatg tcagcagcga cggtaaattt atggtaatgg tcagctccaa
     1681 cggtgggcag cagcacattg ccaaacaaga tctggcaacg ggaggcgtac aagttctgtc
     1741 gtccacgttc ctggatgaaa cgccaagtct ggcacctaac ggcactatgg taatctacag
     1801 ctcttctcag gggatgggat ccgtgctgaa tttggtttct acagatgggc gtttcaaagc
     1861 gcgtcttccg gcaactgatg gacaggtcaa attccctgcc tggtcgccgt atctgtgata
     1921 ataattaatt gaatagtaaa ggaatcattg aaatgcaact gaacaaagtg ctgaaagggc
     1981 tgatgattgc tctgcctgtt atggcaattg cggcatgttc ttccaacaag aacgccagca
     2041 atgacggcag cgaaggcatg ctgggtgccg gcactggtat ggatgcgaac ggcggcaacg
     2101 gcaacatgtc ttccgaagag caggctcgtc tgcaaatgca acagctgcag cagaacaaca
     2161 tcgtttactt cgatctggac aagtacgata tccgttctga cttcgctcaa atgctggatg
     2221 cacatgcaaa cttcctgcgt agcaacccgt cttacaaagt caccgtagaa ggtcacgcgg
     2281 acgaacgtgg tactccggaa tacaacatct ccctgggtga acgtcgtgcg aacgccgtta
     2341 agatgtacct gcagggtaaa ggcgtttctg cagaccagat ctccatcgtt tcttacggta
     2401 aagaaaaacc tgcagtactg ggtcatgacg aagcggcata ctccaaaaac cgtcgtgcgg
     2461 tactggttta ctaagagaat tgcatgagca gtaacttcag acatcaacta ttgagtctgt
     2521 cgttactggt tggtatagcg gccccctggg ccgcttttgc tcaggcacca atcagtagtg
     2581 tcggctcagg ctcggtcgaa gaccgcgtca ctcaacttga gcgtatttct aatgctcaca
     2641 gccagctttt aacccaactc cagcaacaac tttctgataa tcaatccgat attgattccc
     2701 tgcgtggtca gattcaggaa aatcagtatc aactgaatca ggtcgtggag cggcaaaagc
     2761 agatcctgtt gcagatcgac agcctcagca gcggtggtgc agcggcgcaa tcaaccagca
     2821 gcgatcaaag cggtgcggcg gcatcaacga cgccgacagc tgatgctggt actgcgaatg
     2881 ctggcgcgcc ggtgaaaagc ggcgatgcaa acacggatta caatgcagct attgcgctgg
     2941 tgcaggataa atcccgccag gatgacgcaa tggtggcatt tcagaatttc atcaaaaatt
     3001 accctgattc aacttacctg ccaaacgcca attattggct gggtcagtta aactacaaca
     3061 agggtaaaaa agatgatgcg gcgtactatt ttgcttcggt agtgaaaaac tatccgaagt
     3121 caccaaaggc tgctgatgcg atgtttaaag tcggcgtcat catgcaggac aaaggtgaca
     3181 ccgcaaaagc gaaagccgta taccagcagg ttatcagtaa ataccctggt actgatggcg
     3241 ctaaacaggc acaaaaacgt ctgaacgcga tgtaatgcat aacacacgac cagaagtcgc
     3301 attatttctg gtcgtgtcgt gcgaatcata agcagttgag tgatctgcat cgaaattttt
     3361 gttgcgctca agtctgaaat cagtaatata tgccgccgtt gccacgggat atcaaacaaa
     3421 ccgaaagcaa cgaaaaagtg ggtcgttagc tcagttggta gagcagttga cttttaatca
     3481 attggtcgca ggttcgaatc ctgcacgacc caccagtttt agcatcgaag acagatgtta
     3541 agcgtgtagg ataacgttgc gtcagcaacg gcccgtaggg cgagcgaagc gagtcatcct
     3601 gcacgaccca ccactaatta cggtgggttc ggtggaagta gtttgtagta tccagcgcag
     3661 tatcgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
     3721 gatcccgtca tcacccacca ctcgggtcgt tagctcagtt ggtagagcag ttgactttta
     3781 atcaattggt cgcaggttcg aatcctgcac gacccaccaa tcttaaagat tggccccgag
     3841 tagaaatctt tcaggtaaca cccgtatggg tcgttagctc agttggtaga gcagttgact
     3901 tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca ccatcctgaa tgattaaagc
     3961 agcataatcc cgcaa
//
LOCUS       DAZPJR010000257         3961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPJR010000257 DAZPJR010000000
VERSION     DAZPJR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.263"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..1898
                     /gene="tssM"
                     /locus_tag="ACHVB7_004187"
                     /pseudo
     CDS             1..1898
                     /gene="tssM"
                     /locus_tag="ACHVB7_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3358
                     /locus_tag="ACHVB7_004188"
                     /pseudo
     CDS             1909..3358
                     /locus_tag="ACHVB7_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            3285..3767
                     /locus_tag="ACHVB7_004189"
     CDS             3285..3767
                     /locus_tag="ACHVB7_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR1916006.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
BASE COUNT         1063 a         1021 c         1064 g          813 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
     3961 c
//
LOCUS       DAZPJR010000258         3959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPJR010000258 DAZPJR010000000
VERSION     DAZPJR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.264"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHVB7_004190"
     CDS             complement(<1..242)
                     /locus_tag="ACHVB7_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1916007.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHVB7_004191"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHVB7_004191"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR1916008.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="ACHVB7_004192"
     CDS             2255..3904
                     /locus_tag="ACHVB7_004192"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1916009.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1031 a         1001 c          927 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAZPJR010000259         3950 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPJR010000259 DAZPJR010000000
VERSION     DAZPJR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.265"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            753..1181
                     /gene="uspC"
                     /locus_tag="ACHVB7_004193"
     CDS             753..1181
                     /gene="uspC"
                     /locus_tag="ACHVB7_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR1916010.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="ACHVB7_004194"
     CDS             complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="ACHVB7_004194"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR1916011.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="ACHVB7_004195"
     CDS             complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="ACHVB7_004195"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR1916012.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3554..>3950)
                     /gene="araH"
                     /locus_tag="ACHVB7_004196"
     CDS             complement(3554..>3950)
                     /gene="araH"
                     /locus_tag="ACHVB7_004196"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR1916013.1"
                     /translation="RNTLAIGGNEEAARLAGVPVVRTKIIIFVLSGLVSAIAGIILAS
                     RMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVENAMNLLNIS
                     PFAQYVVRGLILLAAVIFDRYKQKAKRIV"
BASE COUNT         1064 a          948 c          885 g         1053 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata aactattttt aaacagacac ttaccacaca
      481 acaaactaat taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac
      541 atacattaac cacttgttta atgcggagaa tcagtacact attttcttat aattacattt
      601 gaaattatat gatcaccggg agtgattgat taattgtctt tactaataat cagactaata
      661 tttacctgtt tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa agctttaatg
      721 tcacctgaag ttcacagaat aaggaacagg ttatgagcta tagcaatatt cttgtcgctg
      781 ttgcggttac accggaaagt cagcaactgc tggcaaaagc ggtatctatc gccaggccag
      841 taaagggaca catcagttta attactctcg cttccgaccc ggaaatgtac aatcaattag
      901 ccgcgccgat gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa agctttcttg
      961 ataagttcat tcaggatgcg gggtatcccg ttgacaagac gtttattgcc tacggtgagt
     1021 taagcgaaca tattctggaa gtatgtcaca aacatcattt cgatttggtg atttgcggta
     1081 atcacaatca cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa
     1141 gtgaggtcga tgtgctgtta gttccactta cgggagatta accgctccta cgcaagcttt
     1201 ggaaaggtag caactttatc gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt
     1261 aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc
     1321 atttctgcat gacgggaaat acgttccgcc agcgacatag tcaatgcacg atccagcgca
     1381 gctgcaactt cgtcacgatc gtaggggtta acaattaacg ccgacgttaa ctcgtttgcc
     1441 gctcccgcaa attgcgaaag aacaagaacg cccgggttgg ctgggtcctg agcagcaaca
     1501 tactcttttg ctaccaggtt catcccgtca cgcagtggcg tcactaagcc cacgtcagag
     1561 tagcggaata ttttcatcag taatttacgg tcaaaatgct gattcaaata ataaagcggc
     1621 gtccagccta attgcccgta tttaccatta attcgtccag cttcattttc gagctgatga
     1681 cgaatatcct gataggcttg cacatcacca cgcgacgttg gtgcaatctg ggtataacga
     1741 attttaccat gatgctgcgg atatttttcc agcaacgctt cataggcgag aaaacgctct
     1801 ggcaaacctt tggaataatc cagccgttcg acagaaaaga tattttgtac gtttttcagt
     1861 tccgctttaa gttgcgccag ttttggcggc agtggcccgg cagcctgttt ggctatttct
     1921 ttcggttcaa tgccgatcgg gtagacttct gttcgaaatg ctttgcccca ggctgtatgg
     1981 ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa gacaatccag gaacgccaga
     2041 cgatcgtttt ctgtctggaa acccagcaaa tcataatcac aaagctgttc aagcaaggtg
     2101 tcatatgtcg gcagcgcgtt gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa
     2161 ccaatgcgat tattcactcc ccgtttgcgt aattcatgcg caaatggcaa caggtgataa
     2221 tcgtggatcc agataatgtc atcgtcttgc aacagcggca gtaatttatc tgccagcaac
     2281 gcatttacgc gtagatagcc gtcccaggca ggacgctgaa attgcaccag atcgagccga
     2341 taatgaaaag cgggccagag aacggcattg gagaattggt tgtagtattc gtcaaggtcc
     2401 tgttcgctga ggttaaaaga ggcccacgta atgttacctt ttttcacctt ttttagcggc
     2461 tgatcctcat tccctgtttc accactccag ccaaaccaca gtccgcctgc ggctttcagt
     2521 gcccccagta tgccaacggc aaggccaccg gcactggcgg cgtgctcgtc tggtggtgca
     2581 atccggttag atactacgac taaacgactc atagtcatca ctcctgttat tttctctttt
     2641 ttgttgtaat gcggtggtta tcatttcaag ccagctccag acatccggca cacccgccag
     2701 tcgccatgat gcctgagttg cacctgtgcc aatttttact gacattccgc ccagtcggtt
     2761 aacgactgcg aagccagatt catcggttaa atcatcgccc agaaatacgg gcgttcgccc
     2821 gataaaggga gcttcctgca taaaagctgc aattgcctca cctttactgg tacctctcgg
     2881 tttgatctcg acaacacact ttccctgctg taacgccatt tgtggccaga tctgagtaat
     2941 acgttgcgct aatgtcatta atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc
     3001 aaaagccatc cctttcgcct ccagctccgc gccgggatac tgagcgatga ctgtatgcag
     3061 ttgcacgcta atatcacgcg caatcgcatc cggcagatga acgatatgtg ttttaccatt
     3121 gatgtcacgg cgctccgccc catgcacgcc cgctaacggg aagcgataag gttttgccag
     3181 tgcgtcaagc tccaccattg agcgccctga tatcaatgcc aatgcaccat cacttgcggt
     3241 tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg acgacctgat cgggatgcgg
     3301 tttgatttcc gccagcgttc catcaagatc aaaaaaccag gcatatttcg cggatagttc
     3361 aggggtttcg gttaacggtt ctgtcacccg gctctcctcc ttctcttttc ttattgtttt
     3421 gcgcagaacg cgctttacag acatgtaagt atagacagtg tgacgggggt cgccatttgc
     3481 aatgaagaac agccaaccgt tgtggcggct ggctgtggtg ggaaaaaacg ttaaattgtt
     3541 gtggaaaaaa gcatcagaca atgcgtttcg ctttttgctt gtaacggtcg aagatcaccg
     3601 ctgccagcag gattaagccg cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt
     3661 tcatggcgtt ttccacggtg cctaaaatta agatacccgc caccacatat gagatttttc
     3721 cgatgccacc tttcagagaa acgccaccta aaacgcaggc ggagataaca ataagctcat
     3781 aaccaatcga cgtcattggc tgcccactag tcatacgtga agccagaata attccggcta
     3841 tcgcagatac caggccagag agaacaaaga taataatttt ggtgcgaaca accggtacac
     3901 ccgccagacg cgcagcctct tcgttcccgc caatcgccag ggtgttacga
//
LOCUS       DAZPJR010000260         3949 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPJR010000260 DAZPJR010000000
VERSION     DAZPJR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.266"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..913)
                     /gene="ydcO"
                     /locus_tag="ACHVB7_004197"
     CDS             complement(<1..913)
                     /gene="ydcO"
                     /locus_tag="ACHVB7_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR1916014.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLF"
     gene            1005..1541
                     /gene="sutR"
                     /locus_tag="ACHVB7_004198"
     CDS             1005..1541
                     /gene="sutR"
                     /locus_tag="ACHVB7_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR1916015.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1614..3575
                     /gene="rlhA"
                     /locus_tag="ACHVB7_004199"
     CDS             1614..3575
                     /gene="rlhA"
                     /locus_tag="ACHVB7_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR1916016.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3667..3897)
                     /locus_tag="ACHVB7_004200"
     CDS             complement(3667..3897)
                     /locus_tag="ACHVB7_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR1916017.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1023 a         1007 c         1001 g          918 t
ORIGIN      
        1 caaacagacc tgcgagcaaa tagaaaatgc ctgcaacggc agctgccagc caacgttgat
       61 ctttatccgg atgcgcttcc gggctttggc aaatagccgc ggtgattgcc gcaataccga
      121 cggaataaac gccgaaaggg gaaaaaacca gtgccagcaa tccagtaaat acaattaatg
      181 gcgaaacagg agccgaatat ccagctgctt tcattgctgc gatacccggt gcgttttgcg
      241 atgccatcgt caccagaaaa agggggagtg caacgctcag gctgtgagca aacgaaaaat
      301 caggggtaat ataagtgggg agaacgggtt taaagacaac atcagttgtg acaacgtcac
      361 cttgcgcgat aacgatcacg atcccaataa tcatcgcggc aattaccgca tagcgcggcg
      421 caacggcctt ggttgccagc cataccagca acatacttcc acacaacgta aattgaccgt
      481 ccagactggc aaacgcctgt aaaccaaagc gtaataaaat cccggcaagc attgccgccg
      541 caagcgagtg cggaataatg cgcatcagac gagcaaagag tcccgttatg ccgcagagga
      601 ctattaacgc gttggtgaca ataaaaacgc cgatggcttc gttaagtgtt agtccctgca
      661 atccggtgac caacaaagcc gcgccaggcg ttgaccatgc ggtgagaaca ggtacgcgat
      721 accataatgt cagagtcagc gtactgacgc ccattgccag ccccagcgcc gtcatccagc
      781 cagagatttg tgcagtggtg gctccggcga caatcgctgc ttgccagatt attgccgctg
      841 aactggcgta gccaattaat accgccagaa accccgccag tagcgtgggt ggaggaatag
      901 aaaacagacg catagtcacc tcgtgcgtta tagcgtacag gcaccgtagc atttgtccgt
      961 tataacgcac aagtgataaa cttccgtttt gccggaggag tcgcatggaa aatctcgctc
     1021 gctttttatc cactacactt aaacaactac gccagcagcg cggctggagt ctttcgcgac
     1081 tggcagaagc gacgggcgtt tctaaagcaa tgttagggca gattgagcgt aatgagtcca
     1141 gtccgacggt cgcgacatta tggaaaattg ccaccgggtt gaacgtacca ttttcaacat
     1201 ttatttctcc gccgcagtcc gctatgcctt cagtttatga tccacaacag caggcgatgg
     1261 tgatcacttc gctgtttcct tatgatccac aactctgctt tgaacacttc tcgattcaga
     1321 tggcatctgg tgcaataagc gaatcgacgc cccatgagaa gggggtaatc gaacatgtcg
     1381 tcgtcattga tggacaactt gatctgtgcg ttgatgggga atggcagacc cttaattgtg
     1441 gggaaggcgt tcgatttgct gcagacgtca cgcatatcta tcgtaatggt ggggagcaaa
     1501 ccgtacattt tcattccctc atccattacc cgcgcagtta agcgggaaaa ctatttcgca
     1561 acgtcgcgct tgtggctaaa atagccgcca tttttcagct actggataag aatgtgaccg
     1621 tatcttctca tcgacttgaa ctgttaagcc cggcacgcga tgccgccatt gcccgcgaag
     1681 ctattttgca cggtgccgat gctgtttata tcggcggccc tggttttggt gcccgtcata
     1741 atgccagtaa tagcctgaaa gatattgccg agctggtgcc gtttgcccat cgttatggtg
     1801 caaaaatttt cgtcacgctt aacaccattt tgcatgatga tgagctggaa cccgcgcaac
     1861 ggctgattac tgacctctac cagaccggtg tcgatgcgct gattgttcag gatatgggga
     1921 ttctggaact tgatattccg ccgattgaac tgcacgccag tacgcagtgc gacattcgta
     1981 cagttgaaaa agcgaagttc ctctctgatg ttggcttcac gcagattgtg ctggcgcgag
     2041 agctgaatct tgatcagatc cgcgcgattc accaggctac ggacgcgacc attgaattct
     2101 ttattcatgg ggcactgtgc gtggcctatt cgggtcagtg ctacatttct catgcgcaaa
     2161 cagggcgtag cgccaaccgt ggcgattgct cgcaggcgtg ccgtttgcca tacacattga
     2221 aagacgatca ggggcgggtg gtttcctatg aaaaacatct gctgtcgatg aaagataacg
     2281 atcagactgc caacctcggc gcgctgattg atgctggcgt acgctccttc aagattgaag
     2341 ggcgttacaa agatatgagc tacgtgaaga atatcaccgc ccattatcgc cagatgctgg
     2401 atgccattat tgaagaacgt ggcgatctga cgcgtgcttc atcaggtcgt actgaacatt
     2461 tctttgttcc atcgacggaa aagactttcc accgtggtag cacagattat tttgtgaatg
     2521 cccgtaaagg cgatattggc gcgttcgatt cgccgaaatt tatcggcctg ccggtgggtg
     2581 aagtattgaa agtggcgaaa gatcatctca atgttgccgt caccgagcca ctggcaaatg
     2641 gtgatggcct gaacgtgatg attaaacgtg aagtcatcgg ttttcgtgcc aatacggtcg
     2701 agaaaaccgg agaaaatcag taccgcgtct ggcccaatga aatgccagca gatttgcaca
     2761 aaattcgtcc acatcaccca ctaaaccgta atcttgatca taactggcag caggcactga
     2821 caaaaacctc cagcgaacgt cgggtggcgg tagacattga actgggcggc tggcaggaac
     2881 aactgattct gaccctcacc agtgaagagg gtgtcagcat cacgcatacg ctggacgggc
     2941 agttcgacga agccaataac gccgaaaaag caatgaacaa tctgaaggat ggtctggcaa
     3001 aactggggca aaccctctat tacgcccgcg atgtgcaaat taatttgccg ggggcgctgt
     3061 ttgtaccaaa cagtctgtta aaccagttcc gccgtgaagc tgctgacatg ctggatgctg
     3121 cgcgtcttgc cagttaccag cgcggcagcc gtaaaccggt tgctgatcct gcgccggttt
     3181 ttccgcaaac gcatctgagt ttcctcgcga acgtatacaa ccagaaagcg cgtgaatttt
     3241 atcatcgcta tggtgtgcag ctgattgacg cggcgtatga ggcacatgaa gagaagggcg
     3301 aagtcccggt gatgatcacc aagcattgtc tgcgctttgc ctttaatctg tgcccgaaac
     3361 aggcgaaagg caatatcaaa agctggaagg cgacgccaat gcaactggtt aacggcgatg
     3421 aagtattaac gctaaagttt gattgccgcc catgcgagat gcacgtcatt ggcaaaatca
     3481 aaaatcacat actgaaaatg ccgttaccgg gaagcgtagt ggcatccgta agtccggatg
     3541 agctgctgaa aacattgcct aagcgaaaag ggtaaaacgc cagttttctg gttactcaca
     3601 actcattgaa tctgcatgat attgcctgcc gggtaaggcg tcacgccgca tccggcatca
     3661 aacgactcaa cgttgtcccg cttctggttt gcgcgatttt tgccagtaaa aatgctcgcg
     3721 caaaccttcc gccgactctt ccgccacagc acgcaattca tcgctgtccg cttcatgacg
     3781 cagctgatga tccacattct ttacccacac aaattcatgt cctttgtgcc ctgccatgag
     3841 ttgtcctgag aacagcgcac acgttaataa gacaaccgat aacgccttcg taaacatcct
     3901 gccacctttt tcttaccttt tgccgctatg atgccgatcg tttcttgag
//
LOCUS       DAZPJR010000261         3922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPJR010000261 DAZPJR010000000
VERSION     DAZPJR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.267"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..301
                     /locus_tag="ACHVB7_004201"
     CDS             <1..301
                     /locus_tag="ACHVB7_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916018.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="ACHVB7_004202"
     CDS             390..2258
                     /locus_tag="ACHVB7_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR1916019.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..3922
                     /locus_tag="ACHVB7_004203"
                     /pseudo
     CDS             2295..3922
                     /locus_tag="ACHVB7_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT         1231 a          721 c          769 g         1201 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaaga agtgagcgta ctgacgaaat cataagtact ctcgaatata
     2941 ttgataatac gttaattacc tttagcaatg aagaaataat taaatgtcaa attgcaatcc
     3001 aatccataat caataaaaac aataatcctt cagagttcag gattatggct actggtcatg
     3061 cccatttaga tatagcgtgg atgtggcctt taagagaagg acgcagaaaa gcaataagaa
     3121 catttgccac cgcccttgca aacattgaaa aatatcctga ttacattttt ggcgctagtc
     3181 agtaccaact ttttcactgg ataaaaaaag attatcccta tttcttcgaa aagttaaaaa
     3241 aacaaatcgc cgcaggccgc tttgagttac aaggttgctt ctgggtcgaa tgcgatttaa
     3301 atctggtaag tggtgaatcg ctgataaggc aaatcatgca tgggacacga ttcacactgc
     3361 agaatttttc aaaaaagata aattatgtct ggcaaccgga tgtctttgga ttccccgcaa
     3421 cgcttccaca aattctcaaa aaatcaggca ttaactatat tgcatcgcaa aagctctcac
     3481 aaaacaagat taataaattt aataattacc ttttccgctg gcagggcctg gatggctcag
     3541 aaattctgat gcataacttt cctgaagaca cttatgatag ccgagcacgt gctcgctctt
     3601 tagaatatat tgaacaaaac tataatgaaa aagagatctg tccctatgcg cttatggtgt
     3661 atggtgtatg gtgtcggtga tggcggcgct ggccccggtg aggaacatat agaacgatta
     3721 acacgtatac gcaatattga tggcttacct catgttgatt tcagccgtgt tgataagttc
     3781 ttcacttacg ccgacgcctt tcgcgaaagc ttgcccatta tttcaggtga actttatttc
     3841 gaggctcatc aagggtgctt tacatcagag tcagcaacta aagctcataa tcgaaatatg
     3901 gaaaataaat tacatgatgc tg
//
LOCUS       DAZPJR010000262         3919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPJR010000262 DAZPJR010000000
VERSION     DAZPJR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.268"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            194..1252
                     /gene="rfaQ"
                     /locus_tag="ACHVB7_004204"
     CDS             194..1252
                     /gene="rfaQ"
                     /locus_tag="ACHVB7_004204"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR1916020.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1249..2373
                     /locus_tag="ACHVB7_004205"
     CDS             1249..2373
                     /locus_tag="ACHVB7_004205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR1916021.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2366..3163
                     /gene="rfaP"
                     /locus_tag="ACHVB7_004206"
     CDS             2366..3163
                     /gene="rfaP"
                     /locus_tag="ACHVB7_004206"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR1916022.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3179..>3919
                     /locus_tag="ACHVB7_004207"
     CDS             3179..>3919
                     /locus_tag="ACHVB7_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1916023.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNY"
BASE COUNT         1031 a          847 c          948 g         1093 t
ORIGIN      
        1 tttgattgat ttctttattg aaaatcaaca atgtgcttaa aaagcattcg ggtcggattg
       61 ccgcgaaaga agtgatacac tagcgcgtca atatcagtgc ggtatcagta ttcaggtagc
      121 tgttgagcct ggggcggtag cgtgcttttt ctgcttcacc ttaatcggat aatctcaaca
      181 aaaagagtta cttgtggata agccatttcg aagaattttg ctcattaaga tgcgttttca
      241 tggggatatg ttattaacta ctcccgtcat tagttcgctg aaaaaaaatt accctgacgc
      301 aaaaatcgat gtgctgcttt atcaggacac caccccgatc ctgtctgaaa atccagagat
      361 taacgcgctc tacggcataa aaaataaaaa agcaaaagcc tcagaaaaaa ttgccaactt
      421 ttttcatctc atcaaggtat tacgtgccaa taagtatgac cttatcgtca atcttaccga
      481 tcaatggatg gttgctatac tggttcgctt attaaatgcc cgtgtgaaaa tttcccagga
      541 ttatcatcat cggcagtctg ctttttggcg taaaagtttc acccatttag tgccgttgca
      601 gggtggaaat gtggtggaaa gtaacttatc cgtgctgaca ccattgggac ttgattcgtt
      661 ggtgaagcag acaaccatga gttacccgcc tgcaagctgg aaacgtatgc gtcgcgaact
      721 tgatcacgct ggtgttggac aaaattatgt ggttatccaa cctacggcgc ggcaaatctt
      781 caaatgctgg gacaacgcca agttttccgc tgtgattgat gccttacatg ctcgtggtta
      841 tgaagtcgtt ctgacgtccg gcccagataa agacgatctg gcctgcgtca atgaaattgc
      901 gcagggatgc cagacgccac cagtaacggc gctggctgga aaggtgacct tcccggaact
      961 tggtgcgtta atcgatcatg cgcagctgtt tattggcgtt gattccgcac cggcgcatat
     1021 tgccgctgca gttaatacgc cgctgatatc gctgtttggt gcgacagacc atattttctg
     1081 gcgtccttgg tcaaataaca tgattcaatt ctgggcggga gattaccggg aaatgccaac
     1141 gcgcgatcag cgtgaccgaa atgagatgta tctttcggtt attccggcgg cagatgtcat
     1201 tgctgctgtc gataaattac tgccctcctc cacgacaggt acgtcgttat gatcgttgct
     1261 ttttgtttat ataaatattt tccctttggc ggtttgcagc gcgattttat gcgtattgct
     1321 cagacagtcg ccgcccgagg tcatcatgtt cgggtttata cccagtcgtg ggaaggcgaa
     1381 tgccctgatg tatttgaact gatcaaagtg ccggttaaat cgcataccaa tcacgggcgc
     1441 aatgcggagt attttgcctg ggtgcaaaaa catttacgcg aacatcccgt cgataaagtc
     1501 gttggattca acaaaatgcc ggggctggac gtttattatg ccgctgatgt ttgttatgcc
     1561 gagaaagtcg cgcaggaaaa aggctttttc tatcacctga cgtcacgtta tcgccattat
     1621 gccgcctttg agcgggcaac cttcgaacag ggcaagccga cacagctgct gatgctgaca
     1681 gataagcaaa tcgccgattt ccagaaacat tatcagactg aagcggagcg ttttcatatt
     1741 ctgccaccgg ggatttatcc tgatcgtaaa tatagccagc agccagctaa tagccgtgaa
     1801 atcttccgta agaagaatgg aataaccgaa caacaatatt tattgttgca ggtcggttca
     1861 gacttcacgc gtaaaggtgt cgatcgttcc attgaagcac ttgcttcgtt accggattcg
     1921 ctgcgccaca acacattgct atatgttgtt ggacaggata agccgcgaaa atttgaggcg
     1981 ctggcagaaa aacgcggcgt gcgcagtaat gttcacttct tctcggggcg caacgatgtc
     2041 tcggaattaa tggcggcggc ggatttatta ctgcatcctg cctaccagga agcagcggga
     2101 attgtgctac tggaagcgat tactgcagga ttaccggtac taacaacagc cgtttgtggc
     2161 tatgcgcatt atattgtcga cgccaattgc ggcgaggcta ttgctgagcc attccgccag
     2221 gaaacattga atgagatttt acgcaaagcg ttaacgcaat cttcattgcg ccaggcctgg
     2281 gcggaaaatg cgcgacatta tgctgataca caagatttat acagtctgcc agagaaagcg
     2341 gcggatatca taacgggtgg tctggatggt tgaacttaaa gagccgtttg ccacgttatg
     2401 gcgcggtaaa gatccttttg aggaagttaa aaccttgcag ggtgaggttt ttcgtgaact
     2461 ggaaactcgc cgcactctgc gatttgaaat ggcgggcaaa agctattttc tcaaatggca
     2521 tcgcggcacg accctgaaag agataatcaa aaatttactc tcattgcgga tgccagtatt
     2581 aggcgcagac cgcgaatgga atgcgattca tcgactgcgg gatgtcggcg ttgatactat
     2641 gtatggggtg gcatttggcg aaaaaggtat taatccgctg agcagaacct cgtttattat
     2701 taccgaagat ctgaccccaa ccatcagtct ggaagattac tgtgctgact gggcgaccaa
     2761 cccaccagat gttcgcgtaa agcgtatgct tattaagcgt gtcgcgacga tggtgcgcga
     2821 tatgcatgct gtgggcatta accaccgtga ctgttatatc tgtcatttcc tgctgcactt
     2881 gcctttttcc ggtaaggaag aggagttaaa aatttcggta attgacctgc accgggcgca
     2941 gcttcgcacg cgcgttccac gtcgttggcg cgataaagat cttattgggc tttatttttc
     3001 ttcgatgaat atcggcctga ctcagcggga tatctggcgg tttatgaaag tgtattttgt
     3061 cgccccgctt aaagacattc tcaagcagga acaaggactg ctgtcgcaag cagaagcaaa
     3121 agccacaaaa atcagggaaa gaacgattcg aaaatcgttg taatttccgg agagaaatat
     3181 gagtgcccac tattttaatc cacaagagat gatcaataag acaatcatct tcgatgaaag
     3241 gccagcggcg tcagtggcat catcattcca tgttgcttat ggcattgata aaaactttct
     3301 ttttggttgt ggtgtttcaa tcacgtcagt tttgttacat aacaacgacg tgagttttgt
     3361 tttccacgtt tttattgatg atatccctga agccgatatc cagcgtttag cccaattggc
     3421 gaaaagctat cgtacctgta tccagatcca tctggtaaat tgtgaacggc ttaaggcatt
     3481 accgacgacc aaaaattggt ctattgccat gtatttccgt tttgtaattg cagattactt
     3541 tattgatcaa caagataaga tcctttacct ggatgctgat atcgcctgtc agggaaactt
     3601 aaagccgctg ataacaatgg atcttgccaa taacgttgct gctgttgtta ctgaacgcga
     3661 tgctaactgg tggtcgttac ggggtcaaag tctgcagtgt aatgaacttg aaaagggtta
     3721 ctttaattca ggtgtcctgt taattaatac actagcgtgg gcgcaggagt ccgtttctgc
     3781 taaagcgatg tcgatgcttg ctgataaagc cgtcgtttcc cgtttaacct atatggatca
     3841 agatatactt aatcttatcc tgtcagggaa agttaaattc attgatgcta aatacaatac
     3901 tcaatttagt ttaaattat
//
LOCUS       DAZPJR010000263         3909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPJR010000263 DAZPJR010000000
VERSION     DAZPJR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.269"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            25..591
                     /locus_tag="ACHVB7_004208"
     CDS             25..591
                     /locus_tag="ACHVB7_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR1916024.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            595..1170
                     /locus_tag="ACHVB7_004209"
     CDS             595..1170
                     /locus_tag="ACHVB7_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR1916025.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1212..2264)
                     /locus_tag="ACHVB7_004210"
     CDS             complement(1212..2264)
                     /locus_tag="ACHVB7_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR1916026.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2489..3409
                     /gene="lpxL"
                     /locus_tag="ACHVB7_004211"
     CDS             2489..3409
                     /gene="lpxL"
                     /locus_tag="ACHVB7_004211"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR1916027.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3581..3766
                     /locus_tag="ACHVB7_004212"
                     /pseudo
     CDS             3581..3766
                     /locus_tag="ACHVB7_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          988 c          951 g          999 t
ORIGIN      
        1 cgcaaattcg tattggatgc acttatgtca ttcacaaata cccctgaacg ctatggtgtt
       61 atatctgcgg ccttccattg gctaagcgca attattgtct acggcatgtt tgctttaggt
      121 ctgtggatgg tcacgctcag ttattacgat ggctggtatc acaaagcacc cgaactgcat
      181 aaaagcatcg gtattttgtt aatgatgggg ctggttattc gcgttctgtg gcgtgtcata
      241 tctccaccgc cgggtccgct gccgagttat tcgcccatga ctcgtcttgc cgcaaaggct
      301 ggacatctcg cattgtacct attgctgttc gccatcggca tcagcggcta tctgatctca
      361 actgccgatg gtaaaccgat cagcgttttt ggctggtttg acgtccccgc gacccttgcc
      421 gacgctggcg cacaggcaga ctttgccggt gccctgcatt tttggctcgc gtggagcgtc
      481 gtagtactgt ccgttatgca cggatttatg gccctgaagc atcatttcat cgataaagac
      541 gacactctga agcgcatgct gggaaagtcg tcatctgact atggagtata aaaaatgaaa
      601 aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc
      661 gccgattaca aaattgacaa agaaggtcag cacgcctttg ttaatttccg catccagcac
      721 cttggctata gctggttata cggcaccttt aaagatttcg acggtacttt tacctttgac
      781 gaaaaaaatc cggctgccga taaagtgaat gtgacaatta acaccaccag cgtcgatact
      841 aatcacgccg aacgcgataa acatcttcgc agtgcagatt tcctcaatac cgcaaaatat
      901 ccacaggcaa cattcacctc caccagcgtg aagaaagacg gtgacgaact ggatattacc
      961 ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt
     1021 cagggcgacg acccatgggg tggtaaacgt gcaggcttcg aggccgaagg caaaattaag
     1081 ctcaaggact tcaatatcaa gacagattta ggtccagctt ctcaggaagt ggatctgatt
     1141 atttcagtgg aaggcgtaca gcagaagtaa gcgtgaaatg ttgccggatg gcacgcatcc
     1201 ggcaatgata tttattctgt tggatcagga atgcccagtg ttgtattcag acgtccacga
     1261 gacttattaa agatcttatt gccattttca cgtcctgccc gacggcgtcg ctgttcttct
     1321 ggcggtaacg cgctttcttc gcagcaaatc tcactacaac aacctttgta tttttccgcg
     1381 catactggac actgaataaa cagcaggtgg cagccatcat ttttacagtt ggtatggctg
     1441 tcgcacggcg cgccgcactg gtggcaatgc gcgataatct catccgatat acgttcgccc
     1501 atccgctcgt caaaaacaaa atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc
     1561 gccttacggg cgtattcaat aattccgccc tcgatatgcc agactttgtt gaatccgtta
     1621 tgtttcatcc aggcactggc cttttcacaa cgaatgccgc cggtgcagta catgacgatt
     1681 tttttatctt tatgtgcctg catcatctcg actgcttttg gcagctgctc acggaaggta
     1741 tctgccggaa tttcgagcgc gttttcaaag tgccccactt catactcata gtggttacgc
     1801 atgtcgataa atagtgcatc gggatcgtca agcatggcgt tcacttccgc cgcttgcaga
     1861 tactcaccaa cattgctggc atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga
     1921 tcgcgtacct tcatgcgcag tacccagaag gatttcccgt catcatccaa cgcgatattc
     1981 aggcgtaaac cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca
     2041 ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat gcgccagata cactcgccca
     2101 aaaacattca gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt aggatcggcg
     2161 atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt cgctctcaac caacattttg
     2221 gcttttaacg cgtcgttgga aatgcggttg tgtaacactg gcatggtgta cggttcctgc
     2281 gagatgggaa agtaaaaatc cgcggcatga tatagcaatt atcgataatt aacatccaca
     2341 cattttacgc tacatttgcg cattaaaaat tatttgttat ttacaagcgc ggcaatttcg
     2401 cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata catacaattg
     2461 cccgaatagg ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact
     2521 gcttcatccg cgttattggt taacctggtt gggtattggc gtactttggt tagtcgtgca
     2581 attgccctac ccggttatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt
     2641 tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag
     2701 cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggactgat
     2761 ggaaaccggt atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat
     2821 cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat
     2881 ccattttctg acactggaac tgggcgcgcg gcagtttggt attcaggaac cggggattgg
     2941 cgtttaccgc ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg
     3001 ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa
     3061 aggcgaagtg gtctggtacg caccggatca tgattacggc ccgcgctcaa gcgttttcgt
     3121 cccgttattt gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat
     3181 gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca
     3241 attgattatg ctgccgccag agtgttctcc gccactggat gatgccgaaa ctaccgccgc
     3301 gtggatgaac aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt
     3361 acaccgtcgc tttaaaacac gcccggaagg cgttccttca cgctattaaa tctcccctgc
     3421 cggatgcttc agaatggcat ccggcattac cacagcaaat ccccctgatt tagcgataaa
     3481 agctctctgg attgcgcccc ctggaagtcg ggcgcataat tagtgtgctt atcttttctt
     3541 cttatgttca ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtggaaatga
     3601 cacccctata aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg
     3661 tgccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc ttggcgttac
     3721 cggtcactcc gccctgaata tgtggtccgg tattgtcttc agtataatac cttcctaaat
     3781 acactacctg agagaagaga tgaaaagagc tatcagctac atgaggtttt caactgagaa
     3841 gcagataaaa ggggactcta tacgaagaca gtctaagctg gtcactgact ggttatcaaa
     3901 gaaccctga
//
LOCUS       DAZPJR010000264         3866 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPJR010000264 DAZPJR010000000
VERSION     DAZPJR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.270"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            752..1468
                     /gene="nanC"
                     /locus_tag="ACHVB7_004213"
     CDS             752..1468
                     /gene="nanC"
                     /locus_tag="ACHVB7_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR1916028.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1488..2594
                     /gene="nanM"
                     /locus_tag="ACHVB7_004214"
     CDS             1488..2594
                     /gene="nanM"
                     /locus_tag="ACHVB7_004214"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR1916029.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2659..3639
                     /locus_tag="ACHVB7_004215"
     CDS             2659..3639
                     /locus_tag="ACHVB7_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR1916030.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3749..3866
                     /locus_tag="ACHVB7_004216"
                     /pseudo
     CDS             3749..3866
                     /locus_tag="ACHVB7_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1163 a          801 c          877 g         1025 t
ORIGIN      
        1 gacttcagaa gcgaccagtt acaagccaca aatcaggaaa catatttcat ggtgagaatt
       61 atgatcagcc tgatggcggg taaacacttg ttttcaccac gctcactgag ttagcttgca
      121 aagcttccct tgtttagacc ctaaataaat tttttataaa acaaacagtt aaaagaaatc
      181 ctttagtttt tgaagaccac ctcaagtgtt cgtctggctt cacatggcat cttcctctta
      241 gaaaaagatc gacatatttt gtgacacgaa ttgcaaatct cgttttgttg tatggcttgc
      301 gtgatttttg atctggtata acaggtataa aggtgcaccg cgatagtcaa tgagacaggg
      361 catctcgcaa tctatggcaa acatcacttc agttctttct catcgggtga tgaaaacgca
      421 cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactctggg
      481 taaaatggat tgagtaagtg atatagctta cgaacattca aatcaattaa acatcagaag
      541 agattttata ctcaggtatt taatctgatt ctctgtttat ttaaataatg tggaaagaga
      601 attttcacag gagaccttat acaaaaatat aaaataagct acccgttgcc aaagacacta
      661 taagcctggc aaaaaatatt acacaacata aatgctaatt gtttatgcgg gctttgtatt
      721 gctttctgta tcctacaaat gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt
      781 attattactg tgcttttcgt caccattaat ttctcaggct gcgacactgg acgtacgtgg
      841 tggatatcgt agcggaagcc atgcctatga gactcgactc aaagtcagtg agggatggca
      901 aaatggatgg tgggcaagca tggaaagtaa tacctggaat accattcatg ataataaaaa
      961 ggaaaatgcc gcactcaatg atgttcaggt tgaagttaat tacgcgatta aacttgatga
     1021 tcaatggacg gtgcgcccgg gaatgttaac gcattttagc agcaacggca cacgctacgg
     1081 accctacgta aaactgtcct gggacgcgac aaaagatctt aattttggca ttcgctatcg
     1141 ttacgactgg aaagcttacc gacaacaaga cttatccggt gatatgtctc gtgataacgt
     1201 tcatcgttgg gatggatatg tcacttacca tattaatagt gatttcacct tcgcatggca
     1261 aacgacgcta tacagcaaac agaatgatta tcgctatgca aaccacaaga aatgggcgac
     1321 ggaaaatgcc tttgttctac aataccatat gacgcccgat attacgccat acatagaata
     1381 tgactacctt gaccgccagg gtgtttacaa cggcagagat aatttatcgg aaaacagtta
     1441 tcgcattggt gtgtcattta aactgtagta gacaggagac agtcacaatg aataaaacta
     1501 taatggcgct tgctatcatg atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa
     1561 ctcctgtgcc atttaaaagt ggtaccggag caattgataa cgacactgtc tacattggtt
     1621 taggtagcgc aggtacagca tggtacaagc tagatacaca ggccaaagat aaaaaatgga
     1681 ctgcgttagc tgcattccct ggtggaccga gagatcaagc aacctctgca tttattgatg
     1741 gcaatctata tgtgtttggc ggcattggca aaaacagcga gggattgact caggtattta
     1801 atgacgtaca caaatacaac cccaaaacca atagctgggt taaattgatg tcgctcgcgc
     1861 cgatgggcat ggcgggtcat gtgacttttg tacacaacgg caaggcttat gttactggcg
     1921 gtgttaacca gaatatcttc aatggctatt ttgaagatct caacgaggct ggaaaagatt
     1981 caaccgctat agataaaatc aacgcccact attttgacaa aaaagcagaa gattatttct
     2041 tcaataagtt tctgttgtct tttgatccct caacacagca atggagttac gctggcgaat
     2101 cgccctggta cggaacggct ggtgcggcgg ttgtgaataa aggtgataaa acctggctta
     2161 ttaatggcga agccaaacca ggattgcgaa cggatgccgt atttgaactt gatttcaccg
     2221 gtaataattt aaaatggaat aagcttgatc ccgtctcatc accagatggc gtcgctggcg
     2281 gttttgcggg gataagcaat gattctctta tatttgccgg aggggccgga ttcaaaggtt
     2341 cacgagaaaa ttaccagaac ggtaagaact atgcgcatga aggcctgaaa aaatcatata
     2401 gcactgatat tcatctttgg cataacggga aatgggataa atcgggtgaa ttatcgcaag
     2461 gtcgggccta cggagtatca ttgccctgga ataatagttt attaattatt ggcggtgaaa
     2521 ctgcaggcgg caaagcggtg acggattcag ttttaatttc tgtgaaggat aataaagtca
     2581 cagtacaaaa ctaacgcttc agggccccgg taaaggggcc ctgctatcaa tttgtcattt
     2641 gtattacgcg aaaattatat gaacgcaata atatcgcccg attattacta tgttcttacc
     2701 gttgctggtc agtctaatgc catggcgtat ggcgaaggac tgccattacc ggacagggaa
     2761 gatgcgcctc attccagaat taaacaatta gcgagatttg cgcatacgca tcccggaggc
     2821 ccgccatgtc actttaacga cattattcca ctgactcact gcccacacga tgttcaggat
     2881 atgcagggtt atcaccatcc tctggcaacg aatcatcaaa cacagtacgg caccgttggc
     2941 caggcactgc atattgcacg gaaattactg cccttcattc ctaataatgc agggattctt
     3001 atcgttccgt gttgccgtgg cggatcggct tttaccgcgg gcagcgaagg gacatattca
     3061 gaacggcacg gagccagcca tgatgcttgt cgttggggaa cggatactcc gctgtaccag
     3121 gatttagtca gcagaacgcg agccgcactg gcaaaaaatc cgcagaacaa attcctcggc
     3181 gtatgctgga tgcaaggcga atttgactta atgaccagtg actacgcgtc acaccctcaa
     3241 cactttaatc atatggttga agcctttcgt agggatctaa aacaatacca ttctcagctt
     3301 aataatatta ctgacgcgcc atggttttgc ggcgatacca cctggtactg gaaagaaaat
     3361 ttcccgcatt cgtatgaagc tatttatggc aattatcaaa ataatgtttt agccaatatt
     3421 attttcgtcg acttccagca acaaggtgaa agaggactga cgaacgcgcc tgatgaagac
     3481 ccggacgatt taagcacggg atattacggt tcagcgtacc ggtcaccgga gaactggacg
     3541 acggcactgc gaagcagtca tttcagcacg gcagcccgtc gggggattat ttctgacaga
     3601 tttgtagaag caattttgca gttttggcgc gaaaggtgag cggggagttt attcatgggg
     3661 gatgtccttg ttcgttctca gagtagacaa caaaaaacaa ataagatcaa ggtaatatct
     3721 cttcacttgc gggctcgaac agcaggcaat tgccaatata ataatctggc acaagggtga
     3781 aaaacgcgca ccgcataaac cgttgttatt gcttacagat tagccggatg ccttaataga
     3841 catccgcgcc ttttcgatta tggctc
//
LOCUS       DAZPJR010000265         3864 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPJR010000265 DAZPJR010000000
VERSION     DAZPJR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.271"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="ACHVB7_004217"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="ACHVB7_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="ACHVB7_004218"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="ACHVB7_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1916031.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..3349
                     /gene="sfmF"
                     /locus_tag="ACHVB7_004219"
     CDS             2834..3349
                     /gene="sfmF"
                     /locus_tag="ACHVB7_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR1916032.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="ACHVB7_004220"
     CDS             complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="ACHVB7_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR1916033.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          988 a          845 c          959 g         1072 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc ttgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccaggtgtg gagaaagcgg atgcgatatt tatgatcaat tataattaat attatattaa
     3361 ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgcaaaccta gtttgccata
     3421 aatattagat ttatgcgcac taactgtttt attgctaagt aataacttat cggcaatttc
     3481 tttattagat aatccgctaa ccagataacg taatatggtc acttcacgat tagatagcac
     3541 agtgaccgtt gaactattcg tactacattt attgcttttt atatagttga gcgtttcgct
     3601 aggaaaaaac gtgtatccgg agaggatcat ctgaacggga tgaaaaatat cattctgatc
     3661 attgcattta ctgacaaaac cgttagcacc agcttgtatc gctctgccag cataaaagca
     3721 ttctgatttc gatgataaaa ataacacttt cactgtgctc tggatttgtt tgatcctttt
     3781 caggaaggta aaaccgtctg ttccgggcaa gtctatatcc ataatgatta aatcaacagg
     3841 acgggttcgg agataatcga tggt
//
LOCUS       DAZPJR010000266         3859 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPJR010000266 DAZPJR010000000
VERSION     DAZPJR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.272"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            116..511
                     /gene="caiF"
                     /locus_tag="ACHVB7_004221"
     CDS             116..511
                     /gene="caiF"
                     /locus_tag="ACHVB7_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR1916034.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(720..1310)
                     /gene="caiE"
                     /locus_tag="ACHVB7_004222"
     CDS             complement(720..1310)
                     /gene="caiE"
                     /locus_tag="ACHVB7_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR1916035.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="ACHVB7_004223"
     CDS             complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="ACHVB7_004223"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR1916036.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2210..3763)
                     /gene="caiC"
                     /locus_tag="ACHVB7_004224"
     CDS             complement(2210..3763)
                     /gene="caiC"
                     /locus_tag="ACHVB7_004224"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR1916037.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          911 a         1050 c          942 g          956 t
ORIGIN      
        1 cttatcgacg gtgaagttgc atactatcga tatatccaca attttaatat ggccttgttt
       61 aattgcttca aaacgagtca tagccagact tttaatttgt gaaactggag ttcgtatgtg
      121 tgaaggatat gttgaaaaac cactctactt gttaatcgcc gaatggatga tggctgaaaa
      181 tcggtgggtg atagcaagag agatctctat tcatttcgat attgaacaca gcaaggcggt
      241 taataccctg acttatattc tgtcggaagt cgcagaaata agctgcgcag ttaagatgat
      301 ccctaataag ctggaaggac ggggatgcca gtgccagcga ctggtaaaag tggtcgatat
      361 cgatgagcaa atttacgcgc gcctgcgcaa taacagccgg gataaattag tcggcgtaag
      421 aaagacgccg cgtattcctg ccgttccgct cacggaactt aaccgcgagc agaagtggca
      481 gatgatgttg tcaaagagta tgcgtcgtta atgtcatttc gttgatacca cgcagcctgg
      541 ttgctggatg caatgctggc gcatcttatc cagcctacgg ttgctctcca gccgggttgg
      601 ataaggcgtt tatgccgcat ccgacagcta tcgccggatg cgatgctggc gcatcttatc
      661 cggcctacgg ttgctctcca gcagagttgg ataaggcgtt tacgccgcat ccggcaagat
      721 caccgtttag gcgtcacatc cgtcgtcccc tgcaaacggg ggcgattttc ctccatttgc
      781 ctcagtggct gtgtttcatg taacgatgca tgacagcgcc cgacaagatc ctgatactct
      841 ttggtattca accgtttcca gtgtaactcg tcgtcactaa cactgcgtac agcgcgggct
      901 ggcgtaccca tcaacaactg gcgtttctcg ccgtgaaagc ctgctttgac aaagctcatg
      961 gcggcaacaa tgctctcttc gccaatgacc gcgccatcca taatcacgct gttcatcccg
     1021 accaatgcat cgcgaccaat cacacaacca tgcaggatcg ctccgtgccc gatatggccg
     1081 ttttccccaa cgatggtgtc agtgtcgcag tagccatgca taatgcagcc atcctgaata
     1141 ttggctcccg cttgcacgat caaccgcccg tagtcaccac gcagtgaggc gagtgggccg
     1201 atgtagacac cggctcccac aatcacatcg ccaatcaaga cggcactggg atggacaaac
     1261 gccgtcgggt gaaccaccgg aattaacccc tcaaaggcgt aatagctcac ggttgttaac
     1321 gtcctttcca caccggatcg cgcttctcgg caaacgccag cggcccttca atggcatcct
     1381 ccgaatgcag aaccgacgga tagtgtttca acacgccgct gcgaatatag cgatacgctt
     1441 cttctaccgg catttcgctg gtggtgcgga agatctcttt cagcgccgca atcgccagcg
     1501 gggcgctgtt aaccagctgc tgcgccagtt cgcgggcgtt atccatcagt tccgcctggc
     1561 taaccacgcg gttgactatc ccccaacgca gcgcctcttc tgcgcccatt cgtctgccgg
     1621 tcatcaccat ttcattgacg atggcaggcg gcaggatctt cggtagacgc agcacgccgc
     1681 cgctgtcagg aacgatgccc agcttggctt ccggcagggc gaagctggcg ttatcggcac
     1741 aaacaataaa atctgccgcc agcgccagtt cgaaaccgcc gtcaaaggca tagccgttca
     1801 cagctgcgat aaccggtttg tcgagattga aaatttcggt taatcccgca aatccacccg
     1861 ggccaaagtc agcatccggt gcttcgcctt ctgctgccgc ttttaaatcc cagcccgcgg
     1921 aaaagaactt ctctccggca ccggtaataa tggcgacacg taattgcgga tcgtcacgga
     1981 aatttagaaa tacttcgccc atttcaaagc tggtttttgc atcaatagca ttcgcttttg
     2041 gacgatcaag ggtaatttcc agaattgatc cattgcgggt cagatgtaat gattcactca
     2101 ttccttttct ccatttttgc tttttcaggg acgacaacat ccctgcaaaa aatgcatatt
     2161 gttttatagc gtgattatta gttggcaggg tagttccctg ctgtttcatt tatttcagat
     2221 tctttctaat tattttcccc gagcaattac gtggcagatc ttttctgatc tccagataag
     2281 agggcacttt aaatttcgcc atattttgtt cgcagaagcg gaaaaattcc tcttcgctca
     2341 atgtttcacc ttcattcagc accacaaatg ctttgatggc ttcatcgcga atcgaatctt
     2401 taatacccac aaccacgata tcctgaattt ttggatgggt ggcgatgata ttttccagct
     2461 ccacgcagga gacattctcg ccaccgcgtt tgatcatatt gcagcggcga tcgacgaaat
     2521 aaaaaaagcc ctcttcgtcg cagtatccgg tatcgccggt atgtagccag ccgtcggctt
     2581 ccagcacttt cgcagtggct ttcgggttga gaaaatactc tttgaagatg gttttccctg
     2641 gtacgccttt aatacagatt tcaccgatct cacctgccgg gagcgggcga ttgtgatcgt
     2701 cgcggatctc cgcttcgtag caaaaccccg cccgaccaat cgacggccag cgtcgtttat
     2761 cgccagggcg atcgccgata atgcccacaa tggtttccgt catcccataa gacgtcagca
     2821 agcgaacgcc gaagcgttca caaaacgcat ctttttcctg ctccgacaag ttgagataaa
     2881 acatcacttc ccgcaggcgg tgttgccgat cgttcgctga aggcggctgc gccatcaagg
     2941 tacggatcat catcggaata cattcggtaa cggtggcgcg gtacttctgt acctgtcccc
     3001 agaaggcgcg ggcgctgtat ttctcgacca gcacaaaggt ggccccggca gaaaacgccg
     3061 ccatcgccgc agtacactgg caatcgatat gaaacgcagg cattaccgtc aggtagacgt
     3121 catcgtcacg cagtgcacac tgccaggcgg agtaatatcc agcgaagcgc aggttgtaat
     3181 gggtaatcac cacacctttc ggtcgggagg tggtgccgga ggtgaagaga atttccgccg
     3241 tatcgtcagt cgatagcggc ggtgcatagc acaaggtggc aggttgttga tttttcagtt
     3301 gagtaaacga actcacgcca tcatcagcgg gaagtgccac atctgtcagg caaatgtgcc
     3361 gcaattgagt ggcatcttcc tgctgaatct gttgatacat aggatagaat tgcgcactgg
     3421 tcaccagcag gcacgcctgg ctattttgca ggatccacgc gctttcctcg cgcaacaggc
     3481 gggcgttaat cggcaccata atcgcgccaa tttttgccag cccgaaccag caaaagataa
     3541 attccgggca gttgtcgaga tgtagagcaa ccttgtcgcc tttgcgaatc cccagcgtat
     3601 aaaacaggtt tgtcgtgcgg ttaatctcct gatttaactc aagataacta taccggttaa
     3661 cgactccgcc gctggattca caaatcagcg ccgttttatg accgtaaacg tccgccagat
     3721 cgtcccacat ttgacgtaga tgttgtccgc caatgatatc cattgcacct ctatccattt
     3781 ttgttcgttt gttattgggc gggcgctagt caggcaagcc gactgacgcc acgcgtttag
     3841 tcctcaactt tggccagac
//
LOCUS       DAZPJR010000267         3833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPJR010000267 DAZPJR010000000
VERSION     DAZPJR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.273"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..266)
                     /locus_tag="ACHVB7_004225"
     CDS             complement(<1..266)
                     /locus_tag="ACHVB7_004225"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1916038.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="ACHVB7_004226"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="ACHVB7_004226"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR1916039.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="ACHVB7_004227"
     CDS             complement(726..998)
                     /locus_tag="ACHVB7_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR1916040.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHVB7_004228"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHVB7_004228"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR1916041.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3733)
                     /locus_tag="ACHVB7_004229"
                     /pseudo
     CDS             complement(2694..3733)
                     /locus_tag="ACHVB7_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          998 a          932 c          917 g          986 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt gcagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcca cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaggc acaataactt tcgctatctt cgatacccca ttgattctct aaagactcgc
     3241 gtcttttact gatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt
     3301 taccaggttc aggctttcgc ggccagaact ctagcgtttc agaccattgc ttatgataga
     3361 atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac
     3421 ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa
     3481 gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat
     3541 tcaaaccgtt ggatgttatc gctgcgaaca caaacattac agcgacaaca catgttaaat
     3601 aaaaccccca cttacaaagc agcatggcct tattttcttt aatcatccgt tcaaaattac
     3661 tattaaatat ttcccagcca ttaaaagaat acttctcgct cccaggatgg ttttgtaata
     3721 aaactttttt catcacattc cctgttatta catactttag aggcgataat tttatccatg
     3781 caaaaaaaat atccaacgaa ttttcttgat ccgctggata tttcagaata tga
//
LOCUS       DAZPJR010000268         3814 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPJR010000268 DAZPJR010000000
VERSION     DAZPJR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.274"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            51..326
                     /gene="ypdI"
                     /locus_tag="ACHVB7_004230"
     CDS             51..326
                     /gene="ypdI"
                     /locus_tag="ACHVB7_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR1916042.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(403..645)
                     /locus_tag="ACHVB7_004231"
     CDS             complement(403..645)
                     /locus_tag="ACHVB7_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR1916043.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            998..1918
                     /gene="lpxP"
                     /locus_tag="ACHVB7_004232"
     CDS             998..1918
                     /gene="lpxP"
                     /locus_tag="ACHVB7_004232"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR1916044.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2274..2345
                     /gene="ypdK"
                     /locus_tag="ACHVB7_004233"
     CDS             2274..2345
                     /gene="ypdK"
                     /locus_tag="ACHVB7_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR1916045.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2410..3648)
                     /gene="alaC"
                     /locus_tag="ACHVB7_004234"
     CDS             complement(2410..3648)
                     /gene="alaC"
                     /locus_tag="ACHVB7_004234"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR1916046.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          985 a          969 c          889 g          971 t
ORIGIN      
        1 agccataaca tcgtatggtt ctcagtgaac cattttcagg ataactttct atgaaagtaa
       61 acttaatact tttcagctta tttttattgg tctctattat ggcatgcaat gtttttgcat
      121 tttccatttc tggtggtgta agtgagagga gctataaaga gactgaaaaa acatcagcga
      181 tgacaaccac acactctaca aaacttcagc catcacaggc gattttgttt aagatgagag
      241 aagatgcgcc accattaaac ctcacagaag aaatcacgcc tacttatccg acaaaggcga
      301 attatcttat tcatcctgtg cgatagcgtc tgtcacatgg caggtgaaca atcatgtctg
      361 atgctgatgt gaacacctgc ccaaatgagc catcctgacc cactatcgcc tgccataccc
      421 caccataaag cgaatcatca agccacaagc cggggcaaga aacataaata tccccgtcac
      481 cagttcatta ccgccagtta accacatggt gaagtaataa atgagtgcag cgaggatcac
      541 catgccgaag aaagaagaga ccgccgatac cacgttcagt acctgaccga tgtagccgct
      601 tacgcagaca atacacaggg cgagaaacat ccataaataa atcatagctc aacctcctgc
      661 cgcgctaaaa aacctgtaac ttcagtcact ttaatagtaa gacaatcctt aacctctggc
      721 aatttttgct aagcatcaat gaaaacagca cgttaaatgc aacgctattg cgtgcggaaa
      781 attttttact ttgcacgatt aataatcagt agctgaaagc agtcagccag tgataaagta
      841 gagccaccgc attcatgctc ctcgtggtta tgtcctgact agtctttaca ctctttacag
      901 gaaccattgt cgtacatgat ggcccaacca attgagtgta ccgctgtcga catggaatct
      961 tctgcgatac aacaattcgt atctacagaa ggtaactatg tttccacaat gcaaattttc
     1021 ccgcgagttt ctacatcctc gctactggct cacatggttt gggcttggtg tactctggct
     1081 ttgggtacag cttccttatc ctgttctctg ctttctcggc acgcgtattg gcgcaatggc
     1141 gcgaccattc ctgaaacgtc gtgaatctat cgcccgtaaa aacctggaac tttgtttccc
     1201 gcagcattct gcggaagaac gcgagaagat gattgccgaa aactttcgtt cactcggcat
     1261 ggcgctggta gaaaccggca tggcatggtt ctggcccgac agtcgcgtac gtaaatggtt
     1321 tgatgttgaa gggttggata accctaaacg cgcacaaatg caaaatcgcg gcgtaatggt
     1381 tgtcggcgtc cattttatgt cgctggaact gggcggccgc gtgatgggac tgtgccaacc
     1441 aatgatggct acctatcgtc cacataataa tcagctgatg gaatgggtgc agacccgtgg
     1501 gcgcatgcgc tctaacaaag cgatgatcgg cagaaataat ctgcgcggca ttgtcggtgc
     1561 actgaagaaa ggtgaagcgg tatggtttgc tcccgatcag gattatggtc gtaaaggcag
     1621 ctccttcgcg ccgttctttg cggtggaaaa tgtcgccaca accaatggca cctatgttct
     1681 ctcccgtctc tctggcgcag ccatgttgac cgtaacgatg gtaagaaaag cggattacag
     1741 cggatatcgt ttgtttatca ccccagagat ggaaggctac ccgacagatg aaaatcaagc
     1801 cgctgcctat atgaacaaga ttatcgagaa agagatcatg cgcgcaccgg agcagtacct
     1861 ctggatccac cgtcgcttta aaacgcgccc ggtgggagaa tcgtcgttgt acatttaaca
     1921 aactgctctt actctttccc tcctcaaatc ggccataata acctcatggt ttttatggct
     1981 tttttcttaa ggttttttcc ttcgctgaat atctaattat taccgtcaga aattaaactg
     2041 tcgccgatat gagacacttg taagttacta aaagtaaata agaaaatgcc tcttgtctcg
     2101 tttcagtttt aggcgtacat taacgccgtc ttgagcagtt tagctcagaa ttctggggtg
     2161 atgcccacgg taaaatacag ggcttttaag gtcgctttgc ccggtgtcaa ctcactgtat
     2221 caggtgtaat gaagtcattc aggcgtaaca gtaattacgc ggagagatgt aaagtgaaat
     2281 atttctttat gggcatttct tttatggtca tcgtttgggc cggtactttt gccctgatga
     2341 tctaaaagca aactgtgcaa tgaaaaacag gagccatcag gctcctgttt gcatttctga
     2401 cgacggtgtt tattccgcgt tttcgtgaat atgtttgctg ctggcgggta gtaaaccgtc
     2461 ggcacggaac atcgctttaa tccctctgat ggcctgacga atacggtcgc ggttttcaat
     2521 cagtgcaaag cgaacatgcg tatcaccata gtcgccaaag ccaatccccg gcgagacaca
     2581 aaccttcgct tcgttaagga gcttcttggc aaattccagc gatcccatgg ccgcatatgg
     2641 ttccgggatt ttcgcccaga catacatcga agccttcggc atttcgacca tccagcccgc
     2701 ttcatgcagc cctttaacca atacatcacg gcggcgtttg tactgttcgg taatgtcgcg
     2761 cacgcactgt tgatcgccct cgagtgccgc aatcgccgcc acctgcaacg gcgtaaaagt
     2821 gccgtaatcg tgatagcttt tgattcgcgc cagggcactg accagcgttt tgttgccgac
     2881 cataaagccg atacgccagc ccgccatgtt gtagcttttc gacagcgtaa agaactcgac
     2941 cgccacatcg cgtgcacccg gcacctgcat aattgacggc gctttccagc catcgtagac
     3001 gatatcggca taggccaggt catggaccac cagcacatcg tagcgtttcg ccagcgccac
     3061 aaccttttcg aagaactcca gttccacgca ttgcgcggtt gggttcgacg ggaagccgag
     3121 gatcatcatc ttcggtttcg gataactttc acgaatggcg cgctccagtt cgttgaagaa
     3181 atcgacacct tccaccagcg gcactgagcg tacctgcgcc ccggcaatca ccgcgccata
     3241 aatatggatc gggtaacttg gattcggcac cagcaccgtg tcaccatgat ccagcgtcgc
     3301 cagcatcaga tgcgccaggc cctctttcga accaatagtg acgatggctt ctgattccgg
     3361 gtcgatttca acgtcgtagc gatcctgata ccagcgggaa atggcgcgac gtaaccgcgg
     3421 aatgccgcgt gaagtggagt aaccatgcgt gtccgggcgc tgggccacag tacataattt
     3481 ttcgacgata tgcggcggag tcgcaccgtc cgggttaccc atgctgaaat cgataatatc
     3541 ttcgccgcgc cgacgcgcag ccattttcag ttcagcggtg atgttaaaaa cgtagggcgg
     3601 gagacgatca atgcgcgtaa agcgacgttc agggcgagtg tcagccataa tttcctcaga
     3661 ttaacgttag cgcccggacc gtccgagcga cgctgccaca atggtggctg ttttgaaaat
     3721 agcctgatta atttctgtct gtccagcgtt ttagaacatt tttatccatg gctttaaatg
     3781 aggaactcca cttcaaaaac aggaaatttt tcta
//
LOCUS       DAZPJR010000269         3744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPJR010000269 DAZPJR010000000
VERSION     DAZPJR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.275"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            3..1198
                     /gene="bluF"
                     /locus_tag="ACHVB7_004235"
                     /pseudo
     CDS             3..1198
                     /gene="bluF"
                     /locus_tag="ACHVB7_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1403..2134
                     /gene="bluR"
                     /locus_tag="ACHVB7_004236"
     CDS             1403..2134
                     /gene="bluR"
                     /locus_tag="ACHVB7_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR1916047.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2355..2759
                     /locus_tag="ACHVB7_004237"
     CDS             2355..2759
                     /locus_tag="ACHVB7_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR1916048.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2812..2922
                     /locus_tag="ACHVB7_004238"
     CDS             2812..2922
                     /locus_tag="ACHVB7_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916049.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3158..3202
                     /locus_tag="ACHVB7_004239"
     CDS             3158..3202
                     /locus_tag="ACHVB7_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916050.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3455..>3744
                     /gene="iraM"
                     /locus_tag="ACHVB7_004240"
     CDS             3455..>3744
                     /gene="iraM"
                     /locus_tag="ACHVB7_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1916051.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNS"
BASE COUNT         1160 a          738 c          747 g         1099 t
ORIGIN      
        1 taatgcttac cacccttatt tatcgtagcc atatacgtga cgacgaacct gtcaaaaaaa
       61 tcgaagaaat ggtttcgata gcaaatcgca ggaacatgca gtctgacgta acagggatct
      121 tactgtttaa tggttctcat tttttccagc ttctggaagg tccggaagaa caggttaaaa
      181 tgatatatcg ggctatatgc caggatccac ggcactataa tattgttgag ctgctgtgcg
      241 attacgcgcc tgctcgccgt tttggcaaag cgggaatgga attatttgat ttgcgcctgc
      301 acgagcgaga tgacgtttta caggccgtat tcgacaaagg cacatcaaaa tttcagctaa
      361 cttatgatga cagagcgcta caattttttc gtacttttgt ccttgcaacc gaacaatcaa
      421 cctatttcga gatccctgcc gaagactcct ggctttttat cgctgacgga tctgataaag
      481 aacttgattc ctgtaccctt tcaccaacta taaacgacca ctttgccttt catcccttat
      541 cgcggcggat aatcgctttt gaagccattg tgcaaaaaaa tgaagcccat cagccatagc
      601 ggttgggcag cgtaaagacg gggaaatcta cacagcggat ctcaaaagta aggcgcttgc
      661 attcgcgatg gcacacgcac ttgagctcgg tgataaaatg atttcaatca atctattacc
      721 tatgaccctg gttaacgaac ctgacgcagt ctctttttta cttgatgaaa taaaggccaa
      781 tgctctggtg cctgaacaaa tcatcgttga atttactgaa agtgaagtta tatctcggtt
      841 tgatgagttt gcagaagcga ttaaatcgct taaggctgtc ggtatcagtg tagcaattga
      901 tcattttggc gcaggttttg ccggtttgtt actcctgtca cgcttccagc ctgacagaat
      961 taaaatcagt caggaattga ttaccaatgt tcataaaagc gggccacggc aggcaattat
     1021 tcaagcgatc ataaaatgct gtacatcact tgaaattcaa gtcagtgcta tgggcgtggc
     1081 aacaccagaa gagtggatgt ggcttgaatc cgcaggaatt gagatgtttc agggagatct
     1141 gtttgctaaa gctaaattga atggtatccc ttccatagcg tggccagaga aaaaataatt
     1201 ttcagcacat tctttcacat gatttcagta aatcatcgtg aaaatgaata cgttatgttt
     1261 aaatttaatc cacattaaat ataatgtttc atccaattta gctgaaatct tatttatact
     1321 ttgtacaatt ttagcgtata ttgtacaaag tattattggg tcgtgtacag gcggcggaga
     1381 tttatgaccg caaggaggaa ttgtggctta ttacagcatt ggtgatgttg ctgaacgttg
     1441 cgggattaat cctgtcactc tccgggcctg gcaacgccgc tacggtttgt taaaaccaca
     1501 gcgcagtgaa ggcggacacc gactctttga tgaagaagac atacaacgca tcgaagagat
     1561 caagcgttgg ataagtaatg gcgtccctgt aggcaaagtt aaagcattac tggaaaccac
     1621 cagccaggat acggaagatg actggaaccg cctgcaagaa gagatgatgt caattcttcg
     1681 tatggctaat cctgccaaac tacgcgcgag aattatttca ctgggtcgag agtacccagt
     1741 agatcaattg attaatcatg tttaccttcc tgttcgccag cgtctcgtgc ttgatcacaa
     1801 cacctcccgc attatgagca gtatgtttga cggcgcatta attgaatatg cagcagcctc
     1861 acttttcgaa atgcgccgta agcccggtaa agaagccatt ctgatggcgt ggaatgttga
     1921 agagagagca cgattgtggc tggaagcatg gcgtttatca ttgtcaggat ggcacatttc
     1981 tgttcttgct gatcccattg aatcgccgca cccggaactg ttcccgacgc aaacattgat
     2041 tgtctggaca ggcatggcac caacgagaag gcaaaacgaa cttttacaac attggggtga
     2101 gcaaggttat aaagtcatct ttcatgcccc ctaatttcac ctttatcccg ccccttagac
     2161 agaatgttat aatctgaacc cacaacagca tttgtaccgg agctggtccg ttgcaaaatg
     2221 ctcaccggaa gaattcaggt aatccctcta tcacactctg tacatatcac agccaaagga
     2281 cagaccagat tgaagtggta aaaccgatcg tgcacactac gcagtctcac aactaacaag
     2341 aggttattat cctgatggat caggtcgttg tttttcaaaa gatgtttgag caagtgagaa
     2401 aagagcaaaa cttctcctgg ttttattcag aattaaaaca tcaccgtatt gcacattaca
     2461 tttattatct ggctacggat aacatcagaa ttattactca cgatgacacg gttttgttat
     2521 taagaggaac caggaacctg ttaaaagtta gtacgaccaa gaaccctgct aaaataaaag
     2581 aggccgcatt gcttcatatt tacggaaaat ctacatttcg ggaatactgt tcaacactgg
     2641 caggcgcggg cgttttccgt tgggttactg atgttaatca taacaaacgc agttactacg
     2701 ccattgataa tacgctttta tatattgaag atgtagaaaa taataaacca ttaatctagc
     2761 taaagttgga tgcttaagaa atgcttcata attcagtaag gcattagcat aatgggaata
     2821 aaagtacaga gactatccct atggatgata aatactgtcc ttttattgct acccataaat
     2881 aatcaccagg ccaatactat caaattgata tttgaaatgt gatcacttga ctttctatac
     2941 gttattttat aacggttaat atatttataa aaacaacggg tgtgccatac gcccgtttca
     3001 atacttaacg cacatgtgtt ttggtttagt catcatccag ttatatgtat tttagccagg
     3061 aacaggttaa acctttccta tataactcaa aaattgaaac cttattctca tgtcatgttt
     3121 atattcatta ttatcgttat ataaaaaggc aaccataatg tttagcaaat tggcaccaag
     3181 tagcataaag gctatgtttt aattacagga tgttcagtca tttgaatgta taacattata
     3241 gctaaacaaa tctaaaacga agtgaataat ttattgtttt cactaaatct cattttgttt
     3301 aacatccatt gagattcctt gctttaaatt ttgttttata taagcaatcg ttttaattaa
     3361 tttatttttt gaaggggtaa tatactcata tgcaaaataa agaaatgaac atccaaatga
     3421 actatattaa ataccgtggg ataagacata acaaatgaag tgggtagtaa ttgacacggt
     3481 aattcaacct acatgcggca tatctttttc agccatatgg ggtgatatga aaaagatcat
     3541 ctggtatcaa tctactatat ttcttcctcc tggaagtata tttacaccgg ttaagcttgg
     3601 tattatcctt aaggaaaaag aatatcctat tactatttat aacattgcac cattcaacaa
     3661 gaatttatgg agtttactca aaagcagtca agagtgtcct ccaggagaaa gcaaaataac
     3721 aaataaatgt ttacataata gttg
//
LOCUS       DAZPJR010000270         3744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPJR010000270 DAZPJR010000000
VERSION     DAZPJR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.276"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1360
                     /gene="dcuC"
                     /locus_tag="ACHVB7_004241"
     CDS             <1..1360
                     /gene="dcuC"
                     /locus_tag="ACHVB7_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1916052.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1401..2081)
                     /gene="dpiA"
                     /locus_tag="ACHVB7_004242"
                     /gene_synonym="citB"
     CDS             complement(1401..2081)
                     /gene="dpiA"
                     /locus_tag="ACHVB7_004242"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR1916053.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2050..3708)
                     /gene="dpiB"
                     /locus_tag="ACHVB7_004243"
     CDS             complement(2050..3708)
                     /gene="dpiB"
                     /locus_tag="ACHVB7_004243"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR1916054.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          852 a          985 c          908 g          999 t
ORIGIN      
        1 tggggttgtg gttattgtgg gtgtagctcg ctacatcatt aaagggtatt ccgccactgg
       61 tgtgttattt gtcggtggcc tgttattgct gattatcagt gccattatgg ggcacaaagt
      121 gttaccgtcc agccaggctt caacaggcta cagcgccacg gatatcgttg aatacgttaa
      181 aatattacta atgagccgcg gcggcgacct cggcatgatg attatgatgc tgtgtggatt
      241 tgccgcttac atgacccata tcggcgcgaa tgatatggtg gtcaagctgg cgtcaaaacc
      301 attgcagtat attaactccc cttacctgct gatgattgcc gcctattttg tcgcctgtct
      361 gatgtctctg gccgtctctt ccgcaaccgg tctgggtgtt ttgctgatgg caaccctatt
      421 tccggtgatg gtaaacgttg gtatcagtcg tggcgcagct gctgccattt gtgcctcccc
      481 ggcggcgatt attctcgcac cgacttcagg ggatgtggtg ctggcggcgc aagcttccga
      541 agtgtcgctg attgacttcg ccttcaaaac gacgctgcct atctcaattg ctgcaattat
      601 cggcatggcg atcgcccact tcttctggca acgttatctg gataaaaaag agcacatctc
      661 tcatgaaatg ttagatgtca gtgaaatcac caccactgct cctgcgtttt atgccatttt
      721 gccgttcacg ccgatcatcg gtgtactgat ttttgacggt aaatggggtc cgcaattaca
      781 catcatcact attctggtga tttgtatgct gattgcctcc attctggagt tcctccgcag
      841 ctttaatacc cagaaagttt tctctggtct ggaagtggct tatcgcggga tggcagatgc
      901 gtttgctaac gtggtgatgc tgctggttgc cgctggggta ttcgctcagg ggcttagcac
      961 catcggcttt attcaaagtc tgatttctat cgctacctcg tttggttcgg cgagtatcat
     1021 cctgatgctg gtattggtga ttctgacaat gctggcggca gtcacgaccg gttcaggcaa
     1081 tgcgccgttt tatgcgtttg ttgagatgat cccgaaactg gcgcactctt ccggcattaa
     1141 cccggcgtat ttgactatcc cgatgctgca ggcgtcaaac ctgggtcgta ccctatcacc
     1201 cgtttctggc gtagtcgttg cggttgccgg gatggcgaaa atctcaccat ttgaagtcgt
     1261 aaaacgcacc tcggtgccgg tgcttgttgg tttggtgatt gttatcgttg ctacagagct
     1321 gatggtgcca ggaacggcag cagcggtcac aggcaagtaa atagtaatgc cggaggcaag
     1381 tttcctccgg ctttatcatg tcacccactg tggtatatgc gttgtggtct gccaactttg
     1441 ccgtgaacaa tttcagcaat aatcagatgg cggctggcgc aatattcaag ataacgcctg
     1501 gcagtggtgc agctgatggt cagtgcctgc gccaccgttt ctgccgtatg ttgcacacca
     1561 ggctctttaa acagttttcg caccgcgttt agcgtcaagg ggtcaatgcc ggtcggtagc
     1621 tcgtccttag gttcaccgcg agcataagca ttaaacatct catcaatttg cttctggctg
     1681 gcgctatcaa tactttccag catatgttta cgctggcgga aacgggttag cgtttgcccc
     1741 agccgttcat aggcaatggg cttaatgaga taatcaaata caccacaacg tacggcttca
     1801 gacaccgttt ccatatcgct ggctgcagtg gtaaacacca cgtcgccggg ataatgcgcc
     1861 tgcaccagtt catgcagtaa attaatccct ctaccgtcag gaagatagtt atcgagcaag
     1921 attagccccg gcttaaaacg ctcgatcatc attcgggcct gcgccagatt tcccgccagt
     1981 aatatctgac tgaatccggg aatgtgacga atatattccg catgcatctc tgccagcggc
     2041 gtttcgtcct caacgatcaa tagggttaat ggagctgtca ttaggtttca ctttcggaat
     2101 atagattgaa aataaggtac cgcagggatc attatcttcg agagtgataa caccaccgca
     2161 gcgcgttacg tagctggcaa tcaagtacaa cccaatgcca tgttcaccgg gctcgtcagc
     2221 acgcgtactg accccctgct caaatatttt gtctcttaga gactctggaa cgccgcagcc
     2281 ctgatcggcg acttcaatca ccacatcatc gccttcatcg ctgaggaata attcaacgat
     2341 cttgtttcct tcatcgctac gcaggctggc ttcgaaggcg ttatcaagta aattgccaac
     2401 aatggcggca aactcggtgc tatccagtcc tggcggcagt tgcgaaagct ggctaccggg
     2461 gacgatgatc atttttagcc ccagttcgcg ggcgcgctgc actttaccaa aaagcagccc
     2521 cgccacctgg cgatcggcaa acgcctcacg caggctgtca ataagctgtt gctgggcctg
     2581 agactccccc tgcaccatcg ccagcacgcg atcatactct ttcatctgca acagaccatt
     2641 gagcgtcgac atccaattgt gatgctcgtg tcgcaatgta cgaaggctct cgacgtattg
     2701 ttttatttgc gtcagttgcg cattgagggt ggaaatttcg tctttactac gaaagctgat
     2761 aatggccccc agcaaatcat cacctgaacg aatagcttcc cggttggcaa taacgctcag
     2821 accattaaag ttcgccacca catcctgacg tttttcatca atctgttcgg taaagaaatc
     2881 ggcgggcctg accacttcag caatgggttt acccaaccat tgccgtccgg gagagctcag
     2941 ccccagcatc tttcttgcgt tacgattgat ggcggtaatg taaccatgcg gatccaccgc
     3001 aatcagccct tcataaaccg aactaaacag cgcctcttgc tgacggacca cgcgtgcgat
     3061 ttgctttggc tccatgccca tcatttgtcg acggatatgc gcggccagga accacgacag
     3121 caacatcaga atccctaaca gcacgacaaa cacgcctgcc atcggtaata aaaactcagc
     3181 ccgccagcta tcgattttac tcaccaggta gccaatcgac accacgccga tgacttttcc
     3241 atcgtcatca aagattggcg ttttggcgcg catcgccatc cccattgccc ctttcccggt
     3301 aatgaagtag ctctcccctt tctccagcgc gccctgtttg gtgaactgca taggataacc
     3361 aattttctcc ggattaagat ggtaaaggcg gatcgagtgc cggtcgccaa tcaccacata
     3421 atcaaaatcg gtatctcttt gtaatttgtt agcgatggtc gccagccgtt tgtagtcacg
     3481 cgttttcacc gcagagatga cactgtcatt ggaggcaata attttcgcct gattcattgc
     3541 catgtcgcgt acatgaagcg ttaaatagtc ctcaaaactg gccgtaaaat attgcgccag
     3601 ggctgcaatg acaaatattg agaacaccag aatcagcaaa aagatacgca gcggaaatgc
     3661 cagtctttgg aaaaatgcaa actgtttatt ctcgttaagc tgcaacattg ttttccttgc
     3721 gggttgtccc gagcctcttt gacg
//
LOCUS       DAZPJR010000271         3736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPJR010000271 DAZPJR010000000
VERSION     DAZPJR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.277"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHVB7_004244"
     CDS             complement(<1..182)
                     /locus_tag="ACHVB7_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR1916055.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="ACHVB7_004245"
     CDS             426..683
                     /locus_tag="ACHVB7_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR1916056.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHVB7_004246"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHVB7_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR1916057.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHVB7_004247"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHVB7_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR1916058.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHVB7_004248"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHVB7_004248"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR1916059.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3736)
                     /locus_tag="ACHVB7_004249"
     CDS             complement(3308..>3736)
                     /locus_tag="ACHVB7_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR1916060.1"
                     /translation="KFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWS
                     ILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLG
                     TYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          954 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaattt
//
LOCUS       DAZPJR010000272         3674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPJR010000272 DAZPJR010000000
VERSION     DAZPJR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.278"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..95
                     /locus_tag="ACHVB7_004250"
     CDS             <1..95
                     /locus_tag="ACHVB7_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1916061.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            244..1491
                     /locus_tag="ACHVB7_004251"
     CDS             244..1491
                     /locus_tag="ACHVB7_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR1916062.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHVB7_004252"
     CDS             complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHVB7_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR1916063.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2343..2687)
                     /locus_tag="ACHVB7_004253"
     CDS             complement(2343..2687)
                     /locus_tag="ACHVB7_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916064.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2929..3531
                     /locus_tag="ACHVB7_004254"
     CDS             2929..3531
                     /locus_tag="ACHVB7_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916065.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          919 a          740 c          845 g         1170 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc cgcttgcagt
      121 aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat agtgagaagt
      181 aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga caggtaggta
      241 attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc cagttactca
      301 tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg gctgaaagga
      361 catctagggt tgacagggac ggaattaggt acactttatt cggtcaacca gtttaccagc
      421 attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc
      481 atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa
      541 ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg
      601 gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt
      661 catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc
      721 tttgccggca tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt
      781 ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg
      841 gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc
      901 tgggttttcg tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa
      961 ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat
     1021 ggttatctca actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc
     1081 tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg
     1141 cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta
     1201 catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt
     1261 gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg
     1321 attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt
     1381 ttcttcgcaa tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt
     1441 aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact
     1501 tttccggttg ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc
     1561 ttagcccggc taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc
     1621 aaatgtactt cagatgcgcg gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct
     1681 gtcccgtgta tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga
     1741 gtcacgccat atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct
     1801 gtttagtcat cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag
     1861 tagatataag gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt
     1921 tgaagggtat ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa
     1981 tgctgttttt tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa
     2041 gagcttattt ttgaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac
     2101 tttggcttta ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc
     2161 agtatcagcc atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat
     2221 gagtgctatc tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga
     2281 tgcagcaatg gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg
     2341 ggttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac
     2401 gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg
     2461 aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca
     2521 acaagtgcaa cgcatcgccg aaagtctgct gcgtcatgga gagcgccgaa gtgatgacca
     2581 tagatattct ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca
     2641 tcgccagttg attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat
     2701 aaaacaaaac tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct
     2761 gttcattgct gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt
     2821 tggtcagttg cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa
     2881 tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt
     2941 ctgtgcaaaa aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac
     3001 atgaaaaaag attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt
     3061 ttccttttct tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata
     3121 tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt
     3181 aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat
     3241 gcagggatat gcatattttc tggatttttt ctgattatac cccttacaac actaacttat
     3301 tttttgcctg gagagacatc gtcttatgtt gcaccgtatc ggtatacttc cggtagttca
     3361 aaaagttgtt ctggagctga ggtggatgac cccgatctac atgagaatat tcgcatttgc
     3421 tatccgtatg gcaattatga gtacgataat attatctatg ttgaaaagaa aattaataca
     3481 ttaggtgcgg tagtgacata tgcacagacc gcgcgtgatg atactgaatg agatagtata
     3541 tagcgggcaa gttttagtta atttatcgag gtaatataat ttacctcgac tcgtttattc
     3601 tggtatgaat atttcgcttt acgatcgatt tttatctgat gatatcatgc ggttttcata
     3661 tactgactta ctgt
//
LOCUS       DAZPJR010000273         3577 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPJR010000273 DAZPJR010000000
VERSION     DAZPJR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.279"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHVB7_004255"
     CDS             <1..196
                     /locus_tag="ACHVB7_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916066.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="ACHVB7_004256"
     CDS             207..374
                     /locus_tag="ACHVB7_004256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916067.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            371..715
                     /locus_tag="ACHVB7_004257"
     CDS             371..715
                     /locus_tag="ACHVB7_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916068.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="ACHVB7_004258"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="ACHVB7_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1916069.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1383..1469
                     /locus_tag="ACHVB7_004259"
                     /pseudo
     CDS             1383..1469
                     /locus_tag="ACHVB7_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1466..1738
                     /locus_tag="ACHVB7_004260"
     CDS             1466..1738
                     /locus_tag="ACHVB7_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916070.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1740..2798
                     /locus_tag="ACHVB7_004261"
     CDS             1740..2798
                     /locus_tag="ACHVB7_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1916071.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2799..3179
                     /locus_tag="ACHVB7_004262"
     CDS             2799..3179
                     /locus_tag="ACHVB7_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1916072.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3176..>3577
                     /locus_tag="ACHVB7_004263"
     CDS             3176..>3577
                     /locus_tag="ACHVB7_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1916073.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          968 a          808 c          980 g          821 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc ttccagacta cacaattctg ataattcagc
     1501 cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt ggtaccagca
     1561 tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca ggcgcggatg
     1621 cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg tcaggctccc
     1681 ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct ggaggtaatg
     1741 tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc gttaagccag
     1801 gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg gaaccaaaaa
     1861 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg gtggaagaca
     1921 aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt ggtgctggtg
     1981 cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac ggcgattatc
     2041 atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     2101 gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct catcaaaacc
     2161 tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag gagcgtgaat
     2221 tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg
     2281 aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct gtgtacagtg
     2341 aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag cgcacaaaaa
     2401 atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa aagacggtgg
     2461 tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag aaatcacaac
     2521 gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg acgtgtggca
     2581 atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg ggaacaaagg
     2641 ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta catgccggag
     2701 taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt tttttaatgc
     2761 acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc atagaatttg
     2821 ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc acatattttg
     2881 tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc cagcagcggc
     2941 tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca ccggataagc
     3001 gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg catgcggggt
     3061 tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag ccagtatctg
     3121 gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc aaaaaatgaa
     3181 actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg atgcatcggc
     3241 ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
     3301 gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
     3361 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
     3421 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
     3481 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct gctgtcacgg
     3541 aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DAZPJR010000274         3543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPJR010000274 DAZPJR010000000
VERSION     DAZPJR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.280"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHVB7_004264"
     CDS             complement(<1..140)
                     /locus_tag="ACHVB7_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR1916074.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHVB7_004265"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHVB7_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR1916075.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2151)
                     /gene="xylB"
                     /locus_tag="ACHVB7_004266"
                     /pseudo
     CDS             complement(696..2151)
                     /gene="xylB"
                     /locus_tag="ACHVB7_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
     gene            complement(2223..>3543)
                     /gene="xylA"
                     /locus_tag="ACHVB7_004267"
     CDS             complement(2223..>3543)
                     /gene="xylA"
                     /locus_tag="ACHVB7_004267"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR1916076.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          923 a          983 c          783 g          854 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaagaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc ccccccccgt
      901 acggtaatcg agcttctgac cgctgatatc cgccagcatc tgacgccagt actcactacg
      961 cgccccgcca ccaatcaacg taacactttg cggtttaatg ccgcaggcat gcacgacatc
     1021 catgccatct gccagcgcat aacccacgcc ttccagcact gctcgcgcca gttcattggg
     1081 gccatgttga tgagtcaaac caaagaaaac ccccttcgcc tggggattat tgtgtggtgt
     1141 acgctcgccg gaaagataag gcagaaacca aactggctcg acactttcat cagcctgttg
     1201 agctgcagcg attaaagctg ggacattgca caggccggtt aatttcgcgg cccaatccag
     1261 acacgatgct gcactcagca ttacagacat taaatgccaa cgctgcggta gcgcatggca
     1321 aaagctgtgt acagcgcttt ctggcttgct taagaaccct tcgctgacag caaaatagac
     1381 ccccgacgtc cccagcgata acattgcctg attagcatca accattccca caccaactgc
     1441 accagctgca ttgtcgccac cgcctgcgac aactggcacc gtcgccatac cccacgcttt
     1501 cgcaacttca ggtaacaaag caccagtaat ttcgctgcct tcgtataatg cgggcatctg
     1561 gtcacgagat aagtcgcaag cctgcagcat gacgtcactc cagtcacgct ttgcgacatc
     1621 cagccacatg gtgccagctg cgtcagacat atcgctggca aactcccccg tcatacgcag
     1681 acgcaagtaa tctttcggta ataatacttt gtcgatttga cggaatatct ccggctcatg
     1741 ccgctgaacc catagcaatt taggcgcagt aaatccgggc atcatcaggt tgccggtaat
     1801 cactcgtgat tgcggaactc tcgcttccag caaagtgcac tcttgcgcac agcgcccgtc
     1861 gttccacaaa atggcagggc gcaatacccg ttgttgagca tccagtaagg ttgctccatg
     1921 catctggccg gcaataccca atgctttaac gtcctgcaga gaatgctgat cgcccagagc
     1981 tttcattgcg cgatcagttg cctgccacca ctgttccggg tcttgttccg accagagtgg
     2041 atgcgggcgc gaaacggtca gcttttccgt ttgcgaatca accacctcac cctgctcgtt
     2101 gagcaaaata acttttacgc ccgaggtgcc aagatctatc ccgatataca tatcgatcgt
     2161 tccttaaaaa aatgcccggt atcgctaccg ataaccgggc caacggactg cacagttagc
     2221 cgttatttgt cgaacagata atgatttacc agattttcca gttgctcctg gcgaccactc
     2281 tgatgcaccg gagacaaatt atgttcctga gcatatttgg ctaaatctgc cagtgacatt
     2341 tggcctttca ggatttgctg gcccaattcg ctattccagc cggaataacg ctgcgcgatg
     2401 cgtttatcca gctcgccatc ttcaatcatg cgcgctgcaa ttttcagcgc cagtgccatc
     2461 gtatccatcg cgccgatatg accgtaaaac agatcatatt tatcagtact ttgacgacgt
     2521 actttggcat cgaagttcag accaccggtg gtgaaaccgc ctgctttgag aatttcatac
     2581 atcaccagcg cattctcttc cacactgttc gggaactggt cggtgtccca gcccagttgc
     2641 gcatcgccac ggttggcgtc gacagaaccg aacaggccaa gcgcaatggc ggtggctatt
     2701 tcatgatgga aagagtgacc tgccagcgtc gcgtggttag cttcaatgtt cagtttaatc
     2761 tctttttcca gaccaaactg tttcaggaag ccatagaccg tcgcggcatc gtaatcatat
     2821 tgatgtttag tcggttcttg cggtttcggt tcgataagca acgtgccctg gaagccgatt
     2881 ttatgtttat gctcaaccac catctgcata aagcggccca gttgttcacg ctcctgacgc
     2941 aagtcggtat ttaacagcgt ttcgtaacct tcacgaccac cccacaggac atagttttca
     3001 ccgcccaatt tatgggttgc ttccatcgct gtaacaactt gcgttgccgc ccagctgaag
     3061 acttcaggat ctgggttcgt cgccgcaccc gcgccatagc gagggtttgt aaagcagtta
     3121 gcggttcccc acagcagctt cacgccgctc tcttcttgct tggctgccag gacatcaacc
     3181 atttgcgcaa aattattgat gtactctttt aacgacgcgc cctcagggga aacatccaca
     3241 tcgtggaagc aataaaatgg cacatgtaac ttgtggaaaa actcaaatgc gacatctgct
     3301 ttacgcttcg ccaacgccag tgcctcacca ggctgctgcc acggacgatt aaacgccccc
     3361 acaccaaaca tgtccgcccc gttccagcag aaggtgtgcc agtagcaggc ggcaaaacgc
     3421 aagtgctctt ccatacgctt acccaacacc agttcgtcgg gattgtagtg acggaatgct
     3481 aacgggtttg aggattttga gccttcataa cgaacgcgat cgagctggtc aaaataggct
     3541 tgc
//
LOCUS       DAZPJR010000275         3523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPJR010000275 DAZPJR010000000
VERSION     DAZPJR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.281"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHVB7_004268"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHVB7_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR1916077.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHVB7_004269"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHVB7_004269"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR1916078.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="ACHVB7_004270"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="ACHVB7_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR1916079.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT          953 a          926 c          827 g          817 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaa
//
LOCUS       DAZPJR010000276         3336 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPJR010000276 DAZPJR010000000
VERSION     DAZPJR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.282"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(334..409)
                     /locus_tag="ACHVB7_004271"
     tRNA            complement(334..409)
                     /locus_tag="ACHVB7_004271"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(374..376),aa:Asn,seq:gtt)
     gene            complement(510..1997)
                     /gene="yeeO"
                     /locus_tag="ACHVB7_004272"
     CDS             complement(510..1997)
                     /gene="yeeO"
                     /locus_tag="ACHVB7_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR1916080.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2158..2233
                     /locus_tag="ACHVB7_004273"
     tRNA            2158..2233
                     /locus_tag="ACHVB7_004273"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2191..2193,aa:Asn,seq:gtt)
     gene            complement(2271..3221)
                     /gene="cbl"
                     /locus_tag="ACHVB7_004274"
     CDS             complement(2271..3221)
                     /gene="cbl"
                     /locus_tag="ACHVB7_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR1916081.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          978 a          865 c          700 g          793 t
ORIGIN      
        1 atttattaaa attccagttc ttctttttct gattacagaa ggcaaagtgg caattacgca
       61 tagtttcccg ataaagacgc gatagcgaca tcccgcataa ggcatttttc tctttatctt
      121 tgtacggtac ttcatggaac agagtttttg accttgcgaa tcgtgatgtc tgttggggag
      181 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
      241 aacccggtca cttttttaca aggtaaccgg gtaaaaataa tttttatttt ttaactgttt
      301 tgagactcat agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag
      361 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
      421 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
      481 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
      541 ttttgcggtt cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
      601 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
      661 cagccaagca tgatgcccag cacataaccg acgacaaccc ggcaacccca catgctcaac
      721 atagaaaccc acatggcgta acgggcatcg cgagcacctt taaatccagc gggtagcacc
      781 catgaggcgg accaaatagg cataaataaa gcatttagcc aaatcagaat cacaacgaca
      841 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
      901 agccaggcga tggccgttaa tccaagagtg gaaagccaga acacatgccg caactgaatc
      961 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagag
     1021 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg aaaacgcgat aaaatttccg
     1081 gcaataacac tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
     1141 aataacactg attcgacact cgcgggaata ccaatcccca tgacttccca gataatgcta
     1201 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
     1261 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccagccct
     1321 gccccgacaa atcccagtcc cggccaggag aaaaggccgt aaatcaatat gccgctaata
     1381 ataatattaa gaatattcag gctaccgtta atcaatagcg gtattttcgt attccctgca
     1441 ccacgaagtg ccccgctacc aataagagtg atggcagctg ctggataact gagtaccgtc
     1501 agctccagat aagtcaacgc cagtgcttta acttctgtcg tggcatcacc cgcgacgaaa
     1561 tcaataattt gttcgccaaa atgatgaata agcgttgcca acagtacggc aaacaacgtc
     1621 atgatcacca atgactgccg cgtcgccacc ctcgctcgtc gtcgatcccg cttaccgaga
     1681 ctaaatgcca caacgacagt agtaccaaga tcgatagcag caaaaaaagc cataatgacc
     1741 atattgaagc tgtccgccaa tcccacgccg gccatcgcat cttttcccag ccagctgacc
     1801 agaaaagtgc tcagaacccc catcaacagg acacaggcat tctccatgaa gataggaaca
     1861 gcaagcgggg ttatctcgcg ccagaacaac actttgtagc tcttgcgttt agcgtgccag
     1921 cgagtgccgt gaacaacctg gcgtaaagca gaggagatat tcaaagccga ccttaattgc
     1981 agaaagtgaa accacatttc aaataatgag ggagaatcag caaagctgca aagattttcg
     2041 ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa acggcaaaca gttggggaat
     2101 ttaaaaatcg ggtttgacaa aagatttttc gccgttaaga tgtgcctcaa cagcgattcc
     2161 tctgtagttc agtcggtaga acggcggact gttaatccgt atgtcactgg ttcgagtcca
     2221 gtcagaggag ccaaatttct gaaaagcccg cttttatagc gggctttttg ctatatctga
     2281 taatcaattt cctcttcact gttttccatc acctgccgct tgatatcctc aactgacagt
     2341 cctgcattac aaagttccag aaagcgccag acatagttac gctgaagttg tcctcgcttc
     2401 agtcccaacc agacagtatt agcatcgaaa agatgccgcg tatccagacg gattaaattc
     2461 tcttcctctt gttcgccgct ggattgttcg gcaactaatc cgatcccaag cccaagagca
     2521 acataggttt taatgacatc agaatcctgc gcacttaata caatatctgc cagcaaacct
     2581 ttgcgggcaa atgcgtcatc aatacgtgag cgccccgtaa tcccctgtcg gtaagtgatt
     2641 aacggccact tcgctattga ttccagcgtc aatggtgtaa tttgcgttaa gggatgatcg
     2701 agtggaacaa gcaaactatg gtgccaacga aaccacggga aggcgacgag ctgcgggtca
     2761 ttactcaaac gctcgctggc gataccaata tcagcttcgc cattttgcaa caacgtcgca
     2821 atttcctgtg gcgtcccctg gattagctcg agccgaacct ccgggaaaag ttcgcgaaaa
     2881 gctttaatga cctctggcaa gctataacgt gcctgagtat gcgtcgttgc aatagtgaga
     2941 acgccagacg tatcgttggt aaacaggtct gcaagccgac gaacattact ggcttcattc
     3001 agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt cagtcatgcc cagcagtcgc
     3061 ttacctcgtc gaacaaatat ttcgatgcca agttcatcct ccagttcccg aatatgacgg
     3121 ctgacgccgg actgtgaggt aaaaagcata ttcgcaacct ctgtcaggtt gtaatcctga
     3181 cgtgcagcct cgcggattat ctttagttgt tggaaattca cggtaaactc cgggcagttc
     3241 agatttcccg ttattgttaa agtctaatgc ccggcataac aaataataaa aacccgcatc
     3301 ttattccatc ccgatataac gcttagctca ccaatt
//
LOCUS       DAZPJR010000277         3294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPJR010000277 DAZPJR010000000
VERSION     DAZPJR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.283"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(89..652)
                     /locus_tag="ACHVB7_004275"
     CDS             complement(89..652)
                     /locus_tag="ACHVB7_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1916082.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="ACHVB7_004276"
     CDS             complement(756..974)
                     /locus_tag="ACHVB7_004276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916083.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="ACHVB7_004277"
     CDS             complement(1133..1519)
                     /locus_tag="ACHVB7_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR1916084.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="ACHVB7_004278"
     CDS             complement(1509..1880)
                     /locus_tag="ACHVB7_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1916085.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="ACHVB7_004279"
     CDS             complement(1880..2137)
                     /locus_tag="ACHVB7_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916086.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..>3294)
                     /locus_tag="ACHVB7_004280"
     CDS             complement(2134..>3294)
                     /locus_tag="ACHVB7_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1916087.1"
                     /translation="ITDTGRKTWVKSSLEHYVAALRRNAALRDAEKTLNEALQKLRDA
                     HTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEVVERVECRNQGLEKSRTLMT
                     GIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKVTEQGHGVLLFTMEMANIQI
                     GERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTGRNIWMVDQANLTIDEICAT
                     TKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISKGLKGLAKSGGFPLIALSQL
                     SRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVYNPDTQATGIAEINITKQRN
                     GSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYSNTRTDSSKMERFF"
BASE COUNT          766 a          915 c          728 g          885 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taat
//
LOCUS       DAZPJR010000278         3285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPJR010000278 DAZPJR010000000
VERSION     DAZPJR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.284"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..229)
                     /locus_tag="ACHVB7_004281"
     CDS             complement(<1..229)
                     /locus_tag="ACHVB7_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916088.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            403..1116
                     /locus_tag="ACHVB7_004282"
     CDS             403..1116
                     /locus_tag="ACHVB7_004282"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1916089.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1148..1906
                     /locus_tag="ACHVB7_004283"
     CDS             1148..1906
                     /locus_tag="ACHVB7_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916090.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1952..>3285
                     /locus_tag="ACHVB7_004284"
     CDS             1952..>3285
                     /locus_tag="ACHVB7_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR1916091.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          750 a          790 c          854 g          891 t
ORIGIN      
        1 atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
       61 acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt
      121 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
      181 aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct
      241 ttaatttaaa catccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat
      301 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
      361 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      421 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      481 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      541 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      601 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      661 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      721 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      781 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      841 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      901 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      961 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
     1021 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
     1081 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
     1141 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
     1201 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
     1261 caccgatctg gtgaaatcat tgcaacagca ctggcaaacc gagcggcgct atctggggct
     1321 gctctcgggc gaagacggcg acaaaatcaa acgattaccg ttggttggcg tctggctgga
     1381 acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
     1441 atgggcggcg gtgattatgt actgcttatc gctgcgaaga atggcgaatc tacgcaaggt
     1501 tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
     1561 ttcgggcttt tattacgacg gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag
     1621 catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat atcgcccgga
     1681 attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc ctgaccatga
     1741 ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata acggcacaaa
     1801 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc
     1861 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac
     1921 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
     1981 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
     2041 cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     2101 catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     2161 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     2221 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     2281 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     2341 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     2401 gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac
     2461 gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc
     2521 gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt
     2581 tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga
     2641 gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     2701 catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc
     2761 tgccgccggg ttatttaaaa tcgccatgac attctttgac gccgccggaa cgccagcggg
     2821 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     2881 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     2941 cgcggtgttg atcgccggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     3001 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc
     3061 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     3121 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     3181 agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac
     3241 tttaaaggca ttttttcagc gtcattcctt gctttataac gcagc
//
LOCUS       DAZPJR010000279         3235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPJR010000279 DAZPJR010000000
VERSION     DAZPJR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.285"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHVB7_004285"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHVB7_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR1916092.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHVB7_004286"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHVB7_004286"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR1916093.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="ACHVB7_004287"
     CDS             1252..1521
                     /locus_tag="ACHVB7_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR1916094.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="ACHVB7_004288"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="ACHVB7_004288"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR1916095.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..3227
                     /gene="dsbA"
                     /locus_tag="ACHVB7_004289"
     CDS             2601..3227
                     /gene="dsbA"
                     /locus_tag="ACHVB7_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR1916096.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          825 a          781 c          786 g          843 t
ORIGIN      
        1 aggccatcct gacggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacac
       61 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      361 cttcgtctgg tttttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      541 ttttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaacaa atgcatcttt
      661 atgatcgctg aaatcaaccg catggccgcc agccagacgc ataaatgcca ttacccggcg
      721 ttctcctgct tgcagatatt ctagtaataa aggctcaata gcacggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcatggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagccgg gaagctaaaa catggggaat gtaaggcgta tcgcacggac aaaacaaaaa
      901 ccactcacca gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatgacgatt
     1021 ggcattaacc acgacgtgag agagctgcgt cataagcgcg tcagcgacat gttgccataa
     1081 tggtttgccg tttaattcaa gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcatcac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1321 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgcggcgtaa ttaaagagta aaagcttgta agcggcgcca ccaaaatcat cgtgaaatga
     1561 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga cgctctgttt gagcaaggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 gcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagctggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag acggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcacaa acagcttttt atccatcgcc cgaccatcgg
     2041 tctgaatgaa tatctcattg agccacgcaa gctgtttgag gacgctacac tgataccttc
     2101 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcaca
     2161 ctggcgggaa gatttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2281 ggatttgtgg atgttgctca atggcgataa agccgagcag cggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttggcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2461 cgcgttcccg aaaaatttcc cgtggttaac cggggaagat tactggctgc gacaaacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttacaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgtccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt cactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtc aaatctctgg
     3061 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggcgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gtatggatac cagcaatatg gatgtttttg
     3181 ttcagcagta tgctgataca gtgaaatatc tgtccgagaa aaaataattc aatgt
//
LOCUS       DAZPJR010000280         3190 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPJR010000280 DAZPJR010000000
VERSION     DAZPJR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.286"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..401)
                     /locus_tag="ACHVB7_004290"
     CDS             complement(<1..401)
                     /locus_tag="ACHVB7_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1916097.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
     gene            453..1058
                     /gene="wrbA"
                     /locus_tag="ACHVB7_004291"
     CDS             453..1058
                     /gene="wrbA"
                     /locus_tag="ACHVB7_004291"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1916098.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            1079..1306
                     /locus_tag="ACHVB7_004292"
     CDS             1079..1306
                     /locus_tag="ACHVB7_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR1916099.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(1344..2585)
                     /gene="agp"
                     /locus_tag="ACHVB7_004293"
     CDS             complement(1344..2585)
                     /gene="agp"
                     /locus_tag="ACHVB7_004293"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR1916100.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2879..>3190)
                     /locus_tag="ACHVB7_004294"
     CDS             complement(2879..>3190)
                     /locus_tag="ACHVB7_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916101.1"
                     /translation="IFNDNKARLVLDTDPEQKVNEVKNFLTALSDSICSLFIVYSNVA
                     SISLGNESFSTDDDLEYGYLINTGNHYDVYLPPELFAQAYELNNKERNAQIDFLTRYA
                     I"
BASE COUNT          817 a          777 c          754 g          842 t
ORIGIN      
        1 aacttcacag ggtctgttaa ggctgacttg tcgtagagtt caccatcacc cagagtcagt
       61 atagagaccc ccttcctcag tatctgcctg acatcatcta tagctgtgtc gatatcagcc
      121 ctactgaagc ggtcaaagtt ctctacaaca atcaaggaac cttctggaat agccccagac
      181 ataacatttt caaacatacg tctgaagtca gaaccttgat tcttatgttg ctggtgaaaa
      241 ccagatttac ccttatcaac atatctgtga gcaggaagaa ggtaataatc gtttttgtgc
      301 ttagagtaga aagcatcaat gaggtttgtt tgccgtctta aggagtctcc tgagtcttgc
      361 tctttagtac tccacctgat gtatgtcact agcagcttca tatcttcgga ctatgcttat
      421 ctaagtggtt cattgaacag gagaacaatg atatgagtgt taaggcaaga atactagtgc
      481 tttattattc catgtacgga catattgaaa cgatggcacg cgcagtcgct gagggtgcaa
      541 gcaaagtcga tggcgcagaa gttgtcgtta agcgtgtacc ggaaaccatg ccgccgcaat
      601 tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg caagaactgg
      661 ccgattacga cgccattatt tttggtacac ctacccgctt tggcaacatg tccggtcaaa
      721 tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc cggcgcacta tacggaaaac
      781 tggcgagcgt ctttagttcc accggtactg gcggcggtca ggaacaaact attacttcaa
      841 cctggacgac ccttgcgcat cacggcatgg tgattgtccc cattggctac gcagcgcagg
      901 aattatttga cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc accatcgcag
      961 gcggtgacgg ctcacgccag ccaagccagg aagaactgtc tattgctcgt tatcaagggg
     1021 aatatgtcgc aggtctggca gttaaactta acggctaatc ttcaacagga ggatacgcat
     1081 gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc
     1141 gccggaaata gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa tggcagaaaa
     1201 gatttgggaa gaaggtagcg atgaagtgtt agtcaaagcc tttgctaagg ccgataaaga
     1261 ttcgcttttt tggggcgaac agaccatcga acgtaaaaac gtttaaccag cgactccccc
     1321 gcttctcgcg ggggagtttt ctgttatttc accgcttcat tcaacacgct atcaaattta
     1381 tccatcgggc agaaaccgtt ggcatctatc gggcaaccgc ttaattccag cgtcacacgc
     1441 tgagcaggtg cctgcagggt taacgcatcg gcattacgta actgttccgc actctgatac
     1501 acatattcaa ttttcatcaa atcgcgattg gctttgctgt catgccaacg ctggaaaacg
     1561 attttgccgc caatcggcgt gcgttcgttc tggtcatgca actgatacgg tttgaaatcc
     1621 agcgccgtta acagagaggc aatgttggag tcgtgcccaa ccaacactgt aattttcggt
     1681 gcgctggtgc gatcggtgac cagagctttg tcgatataac tgaccagcgg tttcgcaaca
     1741 ttgcgcgcca cttccggtga ggtaaacagg ctgtcctggt agccgttttt aagcttcgac
     1801 aacaccttcc actgctggtc agatttgatt tctccccagg ccacctgatc catcggaaaa
     1861 ccttcgtaat attgcaaagt aaacgcatct accagcgagt tgccgacttt cagcggcccg
     1921 gaaacacctg gttcttgttg atacttggcg ctaaaggtat ttttgccatc caccagcgaa
     1981 cactgttgtt tctctttaca ggcaggggaa tctttatagt taacgatttt ttccagtagc
     2041 tggtagctgt cggtaagctg gagtttgctg agctctttct ccattgccgc caccgcctgt
     2101 tcactgaatg cggcggaatc atcggtgatc accgggttaa aggttgggtc catagtgccc
     2161 attttttcct ggtgatgcac aggaatatca caccccggga atgcgccggt aataaagaac
     2221 tgtgcggtcg caacggtacg ttgcagacta ttggcatagg cataaacggt gtccggcggc
     2281 gggcattccc ccgatttcac catcccctgc tctgccagcc attcacgcat gtaatggccc
     2341 atatacactt cgagcacgcc acctttggtg gtgagttgcc caccggggac gtcccattct
     2401 ggccatttat tcggcgtcga ctgctccagc acactgccat tgttcgccag cggcgcacgt
     2461 aagttatggc ggctcatcat gagcacttgc tgtagctgat agccttccgg tacggtttgt
     2521 gcctgagcat ttgaagcgag taaaactatc cctgccacag ttgcggcgat tagcgttttg
     2581 ttcattcctg gcacctcttt tgttatcaat cattaaagag tgtgacagaa atatgacttt
     2641 tttccgggaa caggttcgca aaatggcttg ttgaacgcct gtttttctat atacatcttc
     2701 aaaaacaggc aaggtttgac gcaaactatg cctgattagc ccctgcctcg cgtacgccgc
     2761 tgataattgc acctgtggat aaacagaata tcagcggcgt tactaataca gaggcaatag
     2821 taccaataat aagagaagtg aggctaataa caatggtttt tagcacatgc ctttgtcatt
     2881 aaattgcata acgagttaaa aaatcaattt gcgcattcct ttccttattg tttaactcgt
     2941 aagcctgagc aaaaagttca ggagggaggt aaacgtcata atgattgcca gtgtttatta
     3001 aatacccata ctcaaggtcg tcatctgttg aaaaggattc attccctaag gagattgatg
     3061 ccacattgct gtaaacaata aataatgagc aaatgctatc tgaaagagca gtgaggaaat
     3121 ttttcacctc attaaccttc tgctctgggt cggtgtctaa aacaaggcgt gccttgttat
     3181 cattaaatat
//
LOCUS       DAZPJR010000281         3175 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPJR010000281 DAZPJR010000000
VERSION     DAZPJR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.287"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            533..2386
                     /locus_tag="ACHVB7_004295"
     CDS             533..2386
                     /locus_tag="ACHVB7_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR1916102.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2537..2752
                     /locus_tag="ACHVB7_004296"
     CDS             2537..2752
                     /locus_tag="ACHVB7_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1916103.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2757..3071
                     /locus_tag="ACHVB7_004297"
     CDS             2757..3071
                     /locus_tag="ACHVB7_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR1916104.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          778 a          762 c          885 g          750 t
ORIGIN      
        1 cttccaggcc catccgctcc ggtgggcctt tttactgcag aaaacaggtt ccccgttaaa
       61 tgctatgttg ctcacaattc agtaagttga cagttgcctg tcagactggg catttgttaa
      121 aaaaatttcg catggtgaat ccccctgagc ggaggggcga ctggtgacgg tataatctct
      181 gattatcaaa acgagaatga cgcgggttta gtggcaccgg gctgaactca ccgggaggca
      241 cccggcacca tgtgcatgat gatacagata cgcggcttta gcccctctcc ggaggggttt
      301 tcttgtgggc aaaaaaagcc cgcgctggga gacgcgggcg gcaaggaata aacaataaaa
      361 cgtgaagtaa tatttcagct ggcgaataat accccatagt aatcactctg cgcaactgcg
      421 cggtcttttt cgaattgcgg gctgtcgtct ctcttctgcc attgtcctgt aacttccgga
      481 cttcagcccg ctccttattt tactcacaat attatcccgg ccgggaggat tcatggcatt
      541 taaacactat gacgtggtca gggcggcatc gccgtcagac cttgcgaaac gactgacaca
      601 aaaactgaag gagggctggc agccgtttgg tagtccggtg gccataaccc cttataccct
      661 gatgcaggcg attgcagcag aaggtgatgt ggtcgtcagt ggtgcaactg agccggagtg
      721 gtactacgtc atcgtactgg ccgggcaatc caatgccatg gcttacggtg aaggtcttcc
      781 gcttccggat tcatacgatg cgccccatcc gcgcattaag caactggccc gtcgtaacac
      841 agtgactccc ggtggtgaag tatgcgtatt taacgacatc attcctgctg accattgtct
      901 gcatgatgtt caggatatga gtacgattaa ccatccccgg gctgacctga gcaaagggca
      961 gtacggctgt gtcggacagg gcttacatat tgccaaaaaa ctgcttccgt atatccctaa
     1021 taatgcgggg atcctgctgg taccatgctg tcgtggtggt tcggcattca cccagggcac
     1081 ggaggggaca ttcagcgagt ccacgggggc cagtcaggat tcggctcgct ggggagtggg
     1141 taagccgtta tatcaggatc tgcttttccg cacgaaggca gcattgcaga aaaacccgaa
     1201 aaacgttttg ctggcgatat gctggatgca gggggaattc gatatgacga atgccagtta
     1261 cgcccagcag ccagcagcat ttcttgcaat ggtacagcag ttccgtgctg accttgccgg
     1321 gctggcggcg cagtgtcacg gtggaagtcc ggcatcagtc ccctggattt gtggcgacac
     1381 gacatacgcg tggaaacaag aacacggtac gcaatatgaa gtggtatatg gtgcatataa
     1441 aggtaaagaa tcccagcaga tttattttgt tccctttatg accgatggta gcggagttaa
     1501 tacaccgaca aacaacccgt cagaagatcc tgatattgcc gggtctggtt attacggttc
     1561 ggcatcccga acgaacaaaa actgggtatc atcaaatcgc ccgacgcatt tcagctcatg
     1621 ggcgcgtcgt ggcattattc ccgatcgtat ggcaactgct attctgaacg tagccggtcg
     1681 caccttagcc ttcattagtg gtaaggcacc ggaaatcaaa ccctcgcccg gcggcgacac
     1741 gccatcgggg ccgtctgaag atgcatccat acgcacaatc tccctgttgc cgacagccgg
     1801 agacgctgct gcgcagggct ggagcattaa gaatggcgga gttcagttgt cagatggtgt
     1861 atttaagatc accaagcaga gcaataaagc ctggtccctg acgcgcccgg tggatgacgc
     1921 agtctccctg ctgacacggg gtggcagact gagctgtaag tttcgactgt caggcgcact
     1981 gaccaacaac cagttcggtc tgggaattta tctgtatacc gatgtagcgt tacctgacgt
     2041 cgtggcgatg accgggactg gtaacccgtt cctgatgtcg ttcttcaccc agaccacaga
     2101 cggcaaactg aatctgatgc atcacaagaa agcaggaaac acaaagttgg gcgagttcgg
     2161 gaattacagt aacgactggc tgacgctgga gctggtgttc accgccggca gtgccacggt
     2221 tactccgaaa ctgaatggag tggctggccc ggcattccag gtcataaaag acagtctgac
     2281 actggggctg aatgcgctga cgctgacgga tattaccaaa aatgcagcgt atggcgttga
     2341 gatagaaagt ctggtgctgg agataaatgc accggcatca tcataaaaag tgagccagcc
     2401 aaatggaagg tatcgttaaa ctcaccggta gtgtcagtgg gtcgtctgag atgcctgcat
     2461 gagttatcag agccatcagt acttaactgg tggctttttt tattgttgtc agcttccgga
     2521 taacgggaga cggggtatgt accagatgga aaaaatcaca acaggtgtgt catacaccac
     2581 gtcagcggtg ggaacgggct actggttcct gcagttgctg gacagggttt ccccgtctca
     2641 gtgggcggca ataggcgtgc tggggagtct gctgtttggg ctgctgacat atctgactaa
     2701 cctgtatttc aaaatcagag aggaccgtcg taaggctgca cggggagagt aattcaatga
     2761 ctcaaaacta tgaactgatt gtgaaaggga tccgcaattt tgagaataaa gttacggtaa
     2821 ctttagcgtt acgggacaaa aaacgctttg acggtgaaat ttttgacctg gacatctcgc
     2881 tggaccgtgt tgaaggtgcc gcgctggagt tttatgaggc agcagccaga aggagcatca
     2941 gacaggtctt cctggatgtt gctgccgggt tatgtgaagg ggacgagctg ttgccagaaa
     3001 cgcgcccctg ttcagaggcg cggtatacca taaaaattaa cagttctgat aactcgatta
     3061 cgggttgtta gctttttgca gttggctttc cagtatcttt cattggtagc atcctgataa
     3121 atatccatga gcgcaaaaat caaatacggc ctgtcagctg ctgttctggc gctga
//
LOCUS       DAZPJR010000282         3112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPJR010000282 DAZPJR010000000
VERSION     DAZPJR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.288"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1258)
                     /locus_tag="ACHVB7_004298"
     CDS             complement(<1..1258)
                     /locus_tag="ACHVB7_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR1916105.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLIERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLLN"
     gene            complement(1415..1624)
                     /locus_tag="ACHVB7_004299"
     CDS             complement(1415..1624)
                     /locus_tag="ACHVB7_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916106.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1647..2357)
                     /locus_tag="ACHVB7_004300"
     CDS             complement(1647..2357)
                     /locus_tag="ACHVB7_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1916107.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2873..>3112)
                     /locus_tag="ACHVB7_004301"
     CDS             complement(2873..>3112)
                     /locus_tag="ACHVB7_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916108.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          913 a          653 c          507 g         1039 t
ORIGIN      
        1 aattaagtaa attagtcagt gaccttgtaa agtctgttaa accttcctgt gaaccatcga
       61 acattaaggc cagattatta ttccatgaat tacttaagcg gcctaactgc gtctgagtag
      121 agttaataat cttttcccat ccaccagctt tatcaatcat ccttcccatt tcagcggata
      181 ctttaggaaa aacatcttta gaaagaacct taccgtcttt catggccttg tctaattctt
      241 ccgtagtcat cccaagactt ctggcaaata actgaaaagc acctgccatt ctgtcaccta
      301 attgaccgcg tagctcttca gcctgaatct ttcctttaga tgccatctgc tcgaaagctt
      361 ttaaaatccc gttaatttca tcggtgctta gccccattaa tctggatgta ccaatcacat
      421 tagaaaataa ggattctttc tggtctgcat tcatatcacc agcaccagcg gaaaacttag
      481 caaaactgct taatgtttca atctggtcta caccactact atcagcaaga ttcttaacaa
      541 attctgtagc tcgtttagca ccttcatcac ctaaaacgaa ttgtgccgct cgttgtgatg
      601 cttctttttt cagaccaacc tccataatcc gcctgtaagc ttctaaggct gtctgaaaac
      661 tataaatggc tgtacctgct ttgattgcac tcgtagctaa tacatccaga ctatttgaag
      721 cattttcact gtgattattt ttgctggtgg tcttgttaaa cttagaaagt gtttcacgaa
      781 gagactgatt taactttgct aactcacgat taaaagtttc gtttccaagt gtcttggatt
      841 tactgcttaa gctgtaatac tggcttttaa aatacttctc aagcatagga ttaaagtcac
      901 gaagcttgct catattcgct gagtaagctg ctgtgattgc tctgcttccg ctaattcgtg
      961 gctgtcttgg ttgtctaagt ggctttaaag tatcctcaat ttcagtttta atcttaaacg
     1021 ctctagtgtt tttaagacgc tgctcaatct tggaaacagt tttatcgaca tttctttcta
     1081 ttagtttgag agaatcattt aaaggcgtag gattaactct aaacgactta ttcaatcgct
     1141 tctcaattcg tgatgctacc tgcatcgttt gtttctcggc tttttccaag ccttttaaaa
     1201 ctttctgaga gttaaaactt aactcaatca gaaaatcatc aactttaaat tgtgccattc
     1261 atatttttca cctctttaag cgtgttgtac gtaaattcta tttttcagtg tgggtattag
     1321 ctttacagat ttcacgtagc acacatacaa aaaaagggag agctaccgat gtcactcgat
     1381 aatctctccc cgtatttacc caaccaattc ttttttaatg tcggacaaga taatgtaatt
     1441 ccgtttcacc tatgatttga gcatcagaag gaatacggtc aatacaccac tttggaatct
     1501 tgcgtgtttt tggtttgtgt gaggtgataa cttgacgttt gtttttaatt cttaaggctg
     1561 gcttagtttg taactttaag ttttcacaaa cagaccgaat agtctctaaa cgttcttcaa
     1621 acattaatca cctctttaat tcataatcaa acagccttgc tatacttctg agaaatcttc
     1681 gttaactgct caatatcagc catcaaagca atcttcttct gcgactcata ttcactaaca
     1741 agttctttca tagtatcaag ttctttttta agcttttgct ttaacttatc cagtttaccg
     1801 gaataatgct tagtcaaatt agcttcagct aactgaagaa tctcttcatc tgttttatca
     1861 gtcatcagaa taaattgacc agaacccctt aatgcttgtc tgacatcaat taaccggaac
     1921 ccttcaggga tactttgaag aactttagga acgtcttcaa tcgctgtacc aaaaccatca
     1981 ttaaagtttc cttcataata gataaacggg tttgacttaa tctcgtaagc aatatcatat
     2041 tctgaaggtt tcgaggtttc ttcgaagttc ttaacagcat cgtaagcgac atcaagtcta
     2101 agacgctgtg attttaaagc tgggtaatat tcacgctcaa gctcaaatag tgcttcagtt
     2161 ttgcagatat caggcatttc ttgaggctca ggagacacaa aggaaattac ctttggtgac
     2221 atctttagct cttgctcaag ctcaatgcgc ttgtcatgct cttgacgctg aatagtatgt
     2281 agcggatgtt caccctgtgg tgaccagtct ccgaagctat caaccttttt accgttaata
     2341 tataaatcgt ttgtcattat aatttcctct aagtgtggtt atttcttaat agagcacctt
     2401 caacgccaat acacttagaa gatgacttaa cgatttatta aagatgctct attaagaaaa
     2461 gccctaaaca taatctgtga agggctttgg aggtttgctt ataacgttaa cgaagtttca
     2521 ggaaaacttt ctttaaaagc tctgtttaaa aactcttaaa gaaagcccta actcatgatt
     2581 agggcaaagc tgtaaaggat tcaataatga atgaacaaag actttttgag atagcctctc
     2641 agaccaacct gaaaggctag gacgaaaagc attgcacctt tccaactcag aatatccatt
     2701 catcggatta ttgaaagaaa aaataagaga gaattgctct cccccatata tacaattatg
     2761 tcatgctcaa ggttgtttgt caacagcctt taacagattg gtaacattta tactttaata
     2821 gatgcacctg caatcatacc aatcaataca aaacagataa tcaccaattc cattaatgca
     2881 ccaccttcga atccgtcacc agaatattat cgctatacaa aattgctata gccgccttga
     2941 gatatttttc agggctaacg ctgtatttgt tcgtttcttc gataataaga cgcttcatgt
     3001 ttgagtcggt aacaggaaac ataaaacgcg gttcaccagt ttcaatttcc tgaataaagt
     3061 cttcaatttc ttctgagttt tcagccatta gctttagtaa tttatcaagg tc
//
LOCUS       DAZPJR010000283         3061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPJR010000283 DAZPJR010000000
VERSION     DAZPJR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.289"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..88
                     /locus_tag="ACHVB7_004302"
     CDS             <1..88
                     /locus_tag="ACHVB7_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1916109.1"
                     /translation="GRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            104..418
                     /locus_tag="ACHVB7_004303"
                     /pseudo
     CDS             104..418
                     /locus_tag="ACHVB7_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            520..1974
                     /gene="amn"
                     /locus_tag="ACHVB7_004304"
     CDS             520..1974
                     /gene="amn"
                     /locus_tag="ACHVB7_004304"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR1916110.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2305..3021
                     /locus_tag="ACHVB7_004305"
     CDS             2305..3021
                     /locus_tag="ACHVB7_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1916111.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          835 a          678 c          688 g          860 t
ORIGIN      
        1 gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca
       61 ttatctgaac ataaaacact atcaataagt tagagtcatt accatggcgc ttgaagcaca
      121 atctattttc tggatagttt tcttctccat aatgctggca aacattgcgc atgacatggt
      181 ggtgtgtgtg caacaaccga tgtttaccga aatgtttggt gccagttatc gctatagtgg
      241 cgctggagtc ggttatcagg ttgccagtgt ggttggcggt ggatttacac cttttattgc
      301 cgctgcactc atcacttact ttgccgggaa ctggcatagc gtcgccattt atttgctggc
      361 tggatgcctg atttccgcaa tgaccgcttt gttgatgaaa gacagtcaac gcgcttgata
      421 gcctggcgaa gatcatccga tcttcgcctt acacttttgt ttcacatttc tgtgacatac
      481 tatcggatgt gcggtaattg tatggaacag gagacacaca tgaataataa gggctccggt
      541 ctgaccccag ctcaggcact ggataaactc gacgcgctgt atgagcaatc tgtagtcgca
      601 ttacgcaacg ccattggcaa ctatattaca agtggcgaat tacctgatga aaacgcccgc
      661 aaacaaggtc tttttgtcta tccatcactg accgtaacct gggacggtag cacaaccaat
      721 ccccccaaaa cgcgcgcatt tggtcgtttt actcacgcag gcagctacac caccacgatt
      781 actcgcccta ctctctttcg ttcgtatctt aatgaacaac ttacgttgct gtatcaggat
      841 tatggtgcgc atatctctgt gcaaccctcg cagcatgaaa tcccttatcc ttatgtcatc
      901 gatggctctg aattgacact tgatcgctca atgagcgctg ggttaactcg ctacttcccg
      961 acaacagaac tggcgcaaat tggcgatgaa actgcagacg gcatttatca tccaacggaa
     1021 ttctccccgc tatcgcattt tgatgcgcgc cgcgtcgatt tttccctcgc acggttgcgc
     1081 cattataccg gtacgccagt tgaacatttt cagccgttcg tcttgtttac caactacaca
     1141 cgttatgtgg atgaattcgt tcgttgggga tgcagccaga tcctcgatcc tgatagtcct
     1201 tacattgccc tttcttgtgc tggcgggaac tggatcaccg ccgaaaccga agcgccagaa
     1261 gaagccattt ccgaccttgc atggaaaaaa caccagatgc cagcatggaa tttaattacc
     1321 gccgatgctc agggtattac tctggtgaat attggcgtgg gaccatcaaa tgctaaaacc
     1381 atctgcgatc atctggcagt gctacgcccg gatgtctggt tgatgattgg tcactgtggc
     1441 ggattacgtg aaagtcaggc cattggcgat tatgtacttg cacacgctta tttacgcgat
     1501 gaccacgttc ttgatgcggt tctgccgccc gatattccta ttccgagcat tgctgaagtg
     1561 caacgtgcgc tttatgacgc caccaagctg gtgagcggca ggcccggtga ggaagtcaaa
     1621 cagcggctac gtactggtac tgtggtaacc acagatgaca ggaactggga attacgttac
     1681 tcagcttctg cacttcgttt taacttaagc cgggccgtag caattgatat ggaaagtgca
     1741 accattgccg cgcaaggata tcgtttccgc gtgccatacg ggacactact gtgtgtttca
     1801 gataaaccat tacatggtga gattaaactt cccggtcagg ctaaccgttt ttatgaaggc
     1861 gctatttccg aacatctgca aattggcatt cgggcgatcg atttgctgcg cgcagaaggc
     1921 gaccgactgc attcgcgtaa attacgaacc tttaatgagc cgccgttccg ataataagtt
     1981 ctgaaccttc ttatcagaaa tagatgtgaa ggagtaagta agacacctgg caaatagcct
     2041 gcaataatcg tgggctgttt gcttccttgg gcggatacga gttttattat cgtcttaatg
     2101 atttccacat attaaaagca agtatgcttt caaaacacaa ttataaaaaa tcccgccaac
     2161 aatataagtt tttataaaat tatggcttta gaatattttt atttctaata gacgagattt
     2221 ttcctgttat gatataatat gccgaattaa cacatgttaa cgatttacca gtaatgtaaa
     2281 taaattttcg aggagatcat tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa
     2341 acggctaaag acggtgcaac gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct
     2401 gctgctaaac aaggtgaacc cgatccagaa ttaaacacat ctttaaaatt cgttattgaa
     2461 cgtgcaaagc aggcacaagt tccaaagcac gttattgata aagcaattga taaagccaaa
     2521 ggcggcggag atgaaacgtt cgtgcaggga cgttatgaag gctttggtcc taatggctca
     2581 atgattatcg ccgagacatt gacttcaaat gttaaccgta cgattgctaa cgttcgcaca
     2641 attttcaata aaaaaggcgg caatatcgga gcggcaggtt ctgtcagcta tatgtttgac
     2701 aatgctggtg tgattgtatt taaaggatca gaccctgacc atatttttga aattttactt
     2761 gaagctgaag ttgatgttcg tgatgtgact gaagaagaag gtaacattgt tatttatacc
     2821 gaacctactg accttcataa aggaatcgcg gctctaaaag cagctggaat cactgagttc
     2881 tcaacaacag aattagaaat gattgctcaa tctgaagttg agctttcccc agaagattta
     2941 gaaatctttg aagggcttgt tgatgccctt gaagatgacg acgatgtaca aaaagtttat
     3001 cataacgtcg caaatctcta attatctttt aaagaaatct gtctttacgg cagatttctt
     3061 t
//
LOCUS       DAZPJR010000284         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPJR010000284 DAZPJR010000000
VERSION     DAZPJR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.290"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHVB7_004306"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHVB7_004306"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR1916112.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHVB7_004307"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHVB7_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR1916113.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHVB7_004308"
     CDS             2805..>2992
                     /locus_tag="ACHVB7_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR1916114.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZPJR010000285         2965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPJR010000285 DAZPJR010000000
VERSION     DAZPJR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.291"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..216
                     /locus_tag="ACHVB7_004309"
     CDS             <1..216
                     /locus_tag="ACHVB7_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR1916115.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(161..298)
                     /locus_tag="ACHVB7_004310"
     CDS             complement(161..298)
                     /locus_tag="ACHVB7_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR1916116.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(271..369)
                     /locus_tag="ACHVB7_004311"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="ACHVB7_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(358..597)
                     /locus_tag="ACHVB7_004312"
                     /pseudo
     CDS             complement(358..597)
                     /locus_tag="ACHVB7_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            696..1586
                     /locus_tag="ACHVB7_004313"
                     /pseudo
     CDS             696..1586
                     /locus_tag="ACHVB7_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1589..2122
                     /locus_tag="ACHVB7_004314"
     CDS             1589..2122
                     /locus_tag="ACHVB7_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1916117.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="ACHVB7_004315"
     CDS             complement(2151..2678)
                     /locus_tag="ACHVB7_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1916118.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>2965)
                     /locus_tag="ACHVB7_004316"
     CDS             complement(2680..>2965)
                     /locus_tag="ACHVB7_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1916119.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          837 a          661 c          649 g          818 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agctcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgca
//
LOCUS       DAZPJR010000286         2944 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPJR010000286 DAZPJR010000000
VERSION     DAZPJR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.292"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            45..1120
                     /locus_tag="ACHVB7_004317"
                     /pseudo
     CDS             45..1120
                     /locus_tag="ACHVB7_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1136..1884
                     /locus_tag="ACHVB7_004318"
                     /pseudo
     CDS             1136..1884
                     /locus_tag="ACHVB7_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            1886..>2944
                     /locus_tag="ACHVB7_004319"
     CDS             1886..>2944
                     /locus_tag="ACHVB7_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916120.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          932 a          587 c          613 g          812 t
ORIGIN      
        1 gggcaactgg acgtcaaggt tgaagaagag gacgggccag tctatatatc aatgacacta
       61 accattacat gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc
      121 ttgaatcatt aaggttaaat acatacgtaa gtttggctcg taatacttac tgggacgcca
      181 gcagtaatgt gaattattca ttatcactta gccgcgattt tgatatcggg ccattaaaaa
      241 acgtctccgc ttctctaaca tttagccgaa taaactggga agaagacaac caggatcaac
      301 tgtacctaaa tatttcgatt ccctggggaa ccagtagaac attgagctat ggtatgcaac
      361 gaaatcagga taacaagatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata
      421 attcctggag cgtttctgct tcaggcgaca atgatgaatt caaagatatg aaagcgtcac
      481 tacgcgccag ttatcagcat aataccgaga acggtcgtct ctatctctcc gggacatcac
      541 agcgagacag ttattattct ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc
      601 acggagccgc tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag
      661 atggtgcaga agatattccg ttgaacaata aacgcgcggt aactaatcgg tatggcatcg
      721 gagttattcc atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc
      781 ttccagaaaa tgtggatatc gaaaactcgg ttatcaccac caccttaacc gaaggtgcta
      841 ttggctacgc caaacttgat accccaaggg ctatcaaatc atgggggtta ttcgcctggc
      901 agatggtagt cacccaccac tggggattag cgtaaaagat aaaaccagcc ataaagaatt
      961 aggactggtt gctgatggcg gctttgtata cctcaacggc attcaggatg acagcaaact
     1021 tactttacgc tggggtgata aatcctgttt tattcaacca cccaatagca gcaacttaac
     1081 caccggaacg gttattttac cgtgtattag ccaaaattaa ttcaggatga aactaatgcg
     1141 ctacttaaac acaaagaatc tcattgcagc aggagtatta ctcgcctgca tgagcagcat
     1201 tgcctggggt gccatcatcc cggaccgtac ccgtatcata atgaatgagt cagataaagg
     1261 ggaagcatta aaacttacta atcaaagcaa aaatctaccc tatctggcgc aaacatggat
     1321 agaagatacg aaaggcaata aatcacgtga ctttattgtc acggttcccc ctatggtacg
     1381 tttaaatccc agtgagcaaa ttcaaataag aatgataagt caggaaaaaa ttgctcaact
     1441 gcctaatgac agagaaacgc tcttctattt taacgtgcga gaaataccgc caaaaacaga
     1501 taaaaaaatg tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa
     1561 gctattgaat tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga
     1621 aatgatggtt cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc
     1681 ggaacaaacg gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc
     1741 catgcagata cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat
     1801 tttggtggat taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt
     1861 tccgtagcca aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat
     1921 aggttttagt gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa
     1981 taccgtagtc attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga
     2041 taagatctgg caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc
     2101 cccatcacct ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg
     2161 cggtcaggac ttttataatc agaactccta tatttttggc gtaacataca atggagttga
     2221 ttatgattcg actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc
     2281 gggtaccttt aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca
     2341 attatcagta acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa
     2401 tagagttaat aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat
     2461 gagtggcgca ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa
     2521 taatattcat gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt
     2581 tgactttggt aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag
     2641 tgtagtcatg acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag
     2701 ctttttcact acggatattt tgagtgatta tagccatctg gatatgggta atggactgct
     2761 attgaagata tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc
     2821 ttctttttca tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc
     2881 gaacccggca gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac
     2941 ctat
//
LOCUS       DAZPJR010000287         2939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPJR010000287 DAZPJR010000000
VERSION     DAZPJR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.293"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHVB7_004320"
     CDS             37..264
                     /locus_tag="ACHVB7_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1916121.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHVB7_004321"
     CDS             complement(265..639)
                     /locus_tag="ACHVB7_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR1916122.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHVB7_004322"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHVB7_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHVB7_004323"
     CDS             complement(2099..2674)
                     /locus_tag="ACHVB7_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1916123.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          696 c          688 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctt atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt gctgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt agatttgttg tctatagcta
     2761 tagtgactct tctatgtaga cattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DAZPJR010000288         2893 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPJR010000288 DAZPJR010000000
VERSION     DAZPJR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.294"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHVB7_004324"
     CDS             <1..493
                     /locus_tag="ACHVB7_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1916124.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHVB7_004325"
     CDS             493..1083
                     /locus_tag="ACHVB7_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR1916125.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1832..2110
                     /locus_tag="ACHVB7_004326"
                     /pseudo
     CDS             1832..2110
                     /locus_tag="ACHVB7_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2170..2658
                     /locus_tag="ACHVB7_004327"
     CDS             2170..2658
                     /locus_tag="ACHVB7_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1916126.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2682..>2893
                     /locus_tag="ACHVB7_004328"
     CDS             2682..>2893
                     /locus_tag="ACHVB7_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1916127.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEY"
BASE COUNT          753 a          632 c          824 g          684 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctg
//
LOCUS       DAZPJR010000289         2878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPJR010000289 DAZPJR010000000
VERSION     DAZPJR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.295"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHVB7_004329"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHVB7_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR1916128.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="ACHVB7_004330"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="ACHVB7_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR1916129.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="ACHVB7_004331"
     CDS             1650..2099
                     /locus_tag="ACHVB7_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1916130.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHVB7_004332"
     CDS             complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHVB7_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR1916131.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHVB7_004333"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHVB7_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR1916132.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHVB7_004334"
     CDS             2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHVB7_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR1916133.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          757 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgctag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggcc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttat
//
LOCUS       DAZPJR010000290         2876 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPJR010000290 DAZPJR010000000
VERSION     DAZPJR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.296"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..2694
                     /locus_tag="ACHVB7_004335"
                     /pseudo
     CDS             1..2694
                     /locus_tag="ACHVB7_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2672..>2876
                     /locus_tag="ACHVB7_004336"
     CDS             2672..>2876
                     /locus_tag="ACHVB7_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916134.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          711 a          717 c          938 g          510 t
ORIGIN      
        1 cctcgcgcaa cggtaacatt acgcgctggt tttatgattc ttcccgcagt ggcctgccct
       61 gtgcggtgga agacggaacc ggggtgaggc ggagaataac gcgaaaccgg tacggtcagc
      121 ttcaggcgtt tacggactgc tcggggtaca cgacgcggta tgagtatgac cggtacggtc
      181 aacaaatcgc cgttcaccgg gaagaaggca tcagcactta cagcagttat aacccgcgcg
      241 gtcaactggt cagtcagagg gatgcgcagg ggcgtgaaac ccgttatgag tacagcgccg
      301 caggagacct caccgctatc gttgccccgg acggcagccg cagtgagata cagtatgatg
      361 cgtggggaaa ggccgtcagc accacgcagg gcggtctgac gcgaagcatg gggtatgacg
      421 ccgccgggcg catcactgtg ctgaccaacg agaacggcag ccagtccacg ttccggtatg
      481 acccggtcga caggctgacg gagcagcgcg gctttgacgg ccggacgcaa cgttaccagt
      541 atgacctgac cggaaaactc acgcagagtg aagatgaggg gcttatcacc ctctggcatt
      601 acgatgcgtc ggaccgtatc acccggcgca cggtgaacgg tgaaccggcg gagcagtggc
      661 agtatgacga ccacggctgg ctgacagaaa tcagccacct gagtgaaggc caccgtgtgg
      721 cggtccacta cggctatgac gataaaggcc gtctgacggg cgaacggcag acggtggaga
      781 ccccggagac gggggagatg ctgtgggagc atgagacggg gcacgcgtac agcgaacagg
      841 ggctggcgac ccgtcaggag ccggacggcc tgccgccggt ggagtggctg acgtatggca
      901 gcggttatct tgcggggatg aagctgggcg gaacaccgct ggtcgagtac acgcgggacc
      961 ggctgcaccg tgagacggcc cgcagcttcg gcggggaggc atatgaactt gccaccgcct
     1021 ggaataccag cggccagctc cggagcaggc acctgaacct tccgcagctt gaccgtgact
     1081 acgactggaa cgacaacgga cagctaatcc gcatcagtgg cccgcaggag agccgggagt
     1141 accgttacag cgacacgggg aggctgacgg gcgtccacac cacggcagcg aacctggata
     1201 tcgatatccc gtatgcaaca gacccggcag gaaatcggct gccggacccg gaactgcacc
     1261 cggacagcac cctcacggcg tggccggata accgcatcgc ggaagatgcg cactatgtct
     1321 atcgctacga tgaatacggc aggctggcgg agaagacgga ccgtatcccg gaaggggtta
     1381 tccggatgca cgacgagcgc acccaccact atcactacga cagccagcac cgcctggtgt
     1441 tctacacgcg gatacagcac ggcgaaccac aggtggagag ccggtacctc tatgacccgc
     1501 tgggccgccg gacgggaaaa cgggtatggc ggcgggagcg tgacctgacg gggtggatgt
     1561 cgctgtcgcg taaaccggag gagacctggt acgggtggga cggtgacagg ctgaccactg
     1621 tacagaccca acagacaaga atccagacgg tataccagcc gggaagcttc acgccgctcc
     1681 tgagaatcga aacagagaat ggcgaacagg cgaaggcgcg gcaccggagc ctggcggagg
     1741 tgttgcagga ggacacgggg gtgacgcttc cggcggagct ggcggtgatg ctggggaggc
     1801 tggagcggga actgcgtgcg ggtgcggtga gtgcggagag tgaggcatgg cttgcgcagt
     1861 gcgggctgac ggtggagcag atggaaagcc agatggaggc ggaatacatc ccggagcgga
     1921 ggctgcatct ttaccactgt gaccaccggg gactgccgca ggcgctcatc agcccggaag
     1981 gggaaacggc gtggcgcggg gagtatgacg agtgggggaa ccagcttaat gaggagaacc
     2041 cgcatcacct gtaccagccg taccgtttgc cggggcagca gtatgatgag gagtcgggac
     2101 tgtattacaa ccgtcaccgg tactacgatc cgttgcaggg gcggtatatc acccaggatc
     2161 cgatagggtt gaagggggga ataaatctat acacgtatcc actggcacca atacgttaca
     2221 ccactggggt tagaacgagt tataagtgta tatgggcctc ccgctccaga ccgggcaggt
     2281 gctgaaactc ctcttgttct tactgatatg actggaggag tgactattta ttatgatcct
     2341 gaaacaggtg attcaatgac ttttgatagt agcaatagaa ttgatcgtcg ttctcaacgt
     2401 ggtgctggtg atccatatac aggtgaagtc gttggttgtg agacaaatgg atcaggaata
     2461 tcggcagcat atggcacaac gaagatctat actacagata ctagagcaag atggctacat
     2521 ggtgggggat catcgttgcg cgatccttat gctcctcgac aaggctggaa accaactatg
     2581 ggatgtaccc gtgcacagaa tgaagatgta gatgagttgt gtaagaaagt gacaagttgg
     2641 atgtattcgc atcctggaga gagaattaga tatgaacgtt ttaagacacg ttaatgcgat
     2701 taacatggga gtgtgcattc taattttggt tattggatgt gcaaatgcta aagttcttaa
     2761 acgatgtata ttacccgctc ctgatggtgg cgtagaaaaa ctatcaaaac ccaacttaca
     2821 tggagtaatt aaaaaagttg atttgaacac aaatgaagcg gaaattaaaa taaaag
//
LOCUS       DAZPJR010000291         2838 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPJR010000291 DAZPJR010000000
VERSION     DAZPJR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.297"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(1118..1453)
                     /locus_tag="ACHVB7_004337"
     CDS             complement(1118..1453)
                     /locus_tag="ACHVB7_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFR1916135.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1582..1929
                     /locus_tag="ACHVB7_004338"
     CDS             1582..1929
                     /locus_tag="ACHVB7_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR1916136.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1949..2539)
                     /locus_tag="ACHVB7_004339"
     CDS             complement(1949..2539)
                     /locus_tag="ACHVB7_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916137.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
     gene            complement(2536..2796)
                     /locus_tag="ACHVB7_004340"
     CDS             complement(2536..2796)
                     /locus_tag="ACHVB7_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916138.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          795 a          671 c          588 g          784 t
ORIGIN      
        1 tcacataaac caccgattga gtgacagtta ctgaaacaac tatttcgaca caaaatctcg
       61 tctattcaac atcatgcaaa tttgtcagtg actgaattta gtgacagaac cctctggaat
      121 tctgccgtgt aaccacttat atgcagacct gccccgttgc tctgggtttt caggctgcga
      181 tgagtgatga gcgatgattt ccatacagat gatatatcga ctggaattga ttgcctgcaa
      241 ttagatttag ttttattgtg tggatcgttt tagttagtgg ggatgaattt ccgataaatt
      301 aaccaatgga aaggagatga tatgaataaa aagataactg gattattaat aattagtctg
      361 gcgtcaattt tcttaattga ttgcacccag tgccattaac atctatctac acaaaaatag
      421 aggaaaacaa ttttgatata tcaaataatt acaagcagct ccattctttt tattgtttgg
      481 gggattacat ctataataat tagaacaaag aattattttt ctaagaagaa aacagacctt
      541 aatataagct tttttatagc cttccctatt tctataacaa tccttttatt ttgccgaggt
      601 atattataaa tataacataa tacatctata tggggtgtac atatccccac ataaaaacac
      661 atactctcat taacacggga agaaactatg ttttccaatt ctcactacat gtacataatc
      721 gttcttgtac tatggtttag tattctcaag tacttacaga gaaagaatag catattacat
      781 cctttatttt cactaatcct gacaccacta accattttaa tcattagact tatgatagat
      841 ttaacataca accaataaat tataaacaaa ataaaaatag ggaacattat atgctttata
      901 aaattttcac attaaacaca aagataaaaa caactatcat tacaatatgt gcattatcat
      961 ataatcttac agcgcaagct tccccgtcat acagacctcg tgatcgagtt aataagacta
     1021 tgatagaact acaaactaac ggagtagaac ggcaactttt agataattct tgtggcataa
     1081 gttctggggt atttaaataa aatataagac aggctgtcta tcttacagac agccttttta
     1141 taataacaga aagaatatat atcatactta acggaagaga taatatgaaa acagtacccg
     1201 tataaacagc aagcaatccc atttttccag gtgtatcagc aaaaaaaacg ctgttccaga
     1261 aatcaggccg ggtaaatttc agagctgtat cttcaatata ccattttgca accggataca
     1321 aaaccattcc gcagagagaa atacaccaga acagtaatct gtatttaaaa tcataatccc
     1381 atgacatata cagcatatat cctcccgtca cccatcccca ccacatatta ttaaaataat
     1441 attttctgtt catctgccag ttttcctttc ttttgcactt ttgtcagtgt actgatgcat
     1501 gacaacataa ccacagcatg ataataataa tcaataacaa taagctgtgt cacgtttaca
     1561 tcatggaacg gaggtgctcc tgtgactttt gagcagaaaa aagcccgggc cattgccctg
     1621 atggacagca aaaagatgtg gcggagcaat tatgcccctc ctcttctgcg tattctctgg
     1681 cggctgggta tccggcttcc gccattaccc tttatgccgt tctggcaggt cacggtgctg
     1741 acggggggct tatggggcat ttcctgggga tgtgcaatgt ggtttatcta ctgggggccg
     1801 tcaggaatgg tggcaggtga ggccattatt ataagtatca cggggggttt tttgtccgga
     1861 ctgctcatgg cctctttcca ctggtggcgc aggaaagtta accgactgcc accatgggat
     1921 gatgtgtaaa ccgtgcacag gcgtgttctt acggagcggc actttcattt atctgtacgc
     1981 gaacctccat tcctgcatcg tgacaggccg gcagccactg cgccacttcc agcggatcgc
     2041 cctcccggcg taccacccag ccttctttat tccataactg cagacaggtg ctgccgtcga
     2101 gacgcaccac aaaatcccca cggcaggcct gatacgttat gccgttcagc tccaccgcca
     2161 gatcagtggc agaagtgccg cctgcgggca gacctgctgc cgggaccagc ggcagtacga
     2221 cgacttctgc cgcacgctgt tctgcccgga tgcggtcctg ttctgccagc agcagaggct
     2281 gtgccacggc ccggccggcg tccgtcagct ccactgccag ctgcaggttc ggagcgcgca
     2341 gggtacgcag ccagccggca tcctccagac gacggcagga ggcgcgcagg tttggcccgt
     2401 agaccggggc ttccccgctt tgctccagca cccgttctat gtcccgcgtc agcacgggtc
     2461 ctggccgttt ttcatcaagg gcagccagca caatcagcac tctccgctgc agtggcgacg
     2521 gtcggcggct ctgagtcatt tccagtcatc ctccggccag atgagctttc tgaggcgctg
     2581 atggtcggcg tcaatgatct cctgtctctt tatggcattc attgaggtat agccatttat
     2641 ggccagttca tgccagaaga tcagcacggc tccggctttg gctgactcgc agagataagt
     2701 tacgccgtgg ctgtccagat tgcattcccg gatgcgtttt tccgcaaacc gggtcagttg
     2761 ttcatagctg agtgaaaacg tcatttcctg atccatcctg cacgttccat ctgataatgt
     2821 tctgacaggt aaaaatca
//
LOCUS       DAZPJR010000292         2759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPJR010000292 DAZPJR010000000
VERSION     DAZPJR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.298"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            137..472
                     /locus_tag="ACHVB7_004341"
     CDS             137..472
                     /locus_tag="ACHVB7_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1916139.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            543..659
                     /locus_tag="ACHVB7_004342"
                     /pseudo
     CDS             543..659
                     /locus_tag="ACHVB7_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(764..840)
                     /locus_tag="ACHVB7_004343"
     tRNA            complement(764..840)
                     /locus_tag="ACHVB7_004343"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(804..806),aa:Arg,seq:tct)
     gene            complement(854..930)
                     /locus_tag="ACHVB7_004344"
     tRNA            complement(854..930)
                     /locus_tag="ACHVB7_004344"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(894..896),aa:Arg,seq:tcg)
     gene            complement(938..1013)
                     /locus_tag="ACHVB7_004345"
     tRNA            complement(938..1013)
                     /locus_tag="ACHVB7_004345"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(978..980),aa:Ile,seq:cat)
     gene            complement(1054..2112)
                     /locus_tag="ACHVB7_004346"
     CDS             complement(1054..2112)
                     /locus_tag="ACHVB7_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR1916140.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(2220..2460)
                     /locus_tag="ACHVB7_004347"
                     /pseudo
     CDS             complement(2220..2460)
                     /locus_tag="ACHVB7_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2685..>2759)
                     /locus_tag="ACHVB7_004348"
     CDS             complement(2685..>2759)
                     /locus_tag="ACHVB7_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1916141.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          739 a          749 c          588 g          683 t
ORIGIN      
        1 acgagatata aaccaccctg catttaaata aacaataaac aacatcagaa aaataattct
       61 gctctatggt ttacattcaa aaatatcatt tatacttttc agaacatcac cagcaaggca
      121 taaacaagga aactaaatga agtggattgt gattgataca gttatccagc catcatgcgg
      181 aatatctttt tcagtcatat ggagtaaaat aaaattaata atctggtatc aatcggatgc
      241 tttcttacct cctgaaagta tatttacact gactcacaca ggcatcatgc tcaataacaa
      301 agtgctacct gtaaccattt acaacgtagt accattcaat aaaacattct ggaattcaat
      361 caaaaatagc caggaatgcc ctacaaatac agataacgta ttgaatgaat gctttaataa
      421 ccgttgcact ctgcaaatat gtccttatgg actaaaacaa caaagtccat aaggagttta
      481 ctcacatctg acaaaatcaa tataaacagc ccctccggag aggggctgga gagtggcgct
      541 atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc agtaccagca cctgaatccg
      601 cgattatccc atatacctac tcgctgattg cccctccgca cagggggatt caccatgcca
      661 gtttctttta acaaactccc cgcaaaacag acaactgtca accgtctgaa ttgtgagaca
      721 tttaaaaaaa aggcccgcaa aagcgagcca gggaaaataa gtgtggcgcg ttgtactgga
      781 ttcgaaccag tgaccgattg cttagaaggc aattgctctg tccggctgag ctaacaacgc
      841 atgatgcaga taatggaccg ccatcgggga cttgaacccc gcgcagccag cttcgaaggc
      901 tggcgctctt tcctgatgag ctaatggcgg tatgtgatgg tggcccttgc tggatttgaa
      961 ccagcgacct ggcgattatg agtcgctcgc tctcaccact gagctaaagg gccgggagcc
     1021 gcataataac gacgcgtaat taattcttca atatcatccg ttctggctga ctaaatcctg
     1081 tacttcccga accgtctgct caaaacgttc agtctccagc tcaacgccaa ttgcacgacg
     1141 cccgagcgcc agtgccgctt tcactgtcga acccaacccc atgaaaaaat ctgcaatcag
     1201 gtcacccgga cgactgctag cgctgattat ctgctgcagc atttctgccg gtttttcgca
     1261 cggatgtttc ccgggataga actgcaccgg tttatgcgtc cacacatcgg tatacggcac
     1321 ctgcaccgtc acgccaaaat accgccgcag atgtttatat tcactcagca gctccgcata
     1381 ctgccggttc agtgaggtat acgtatccac cagctggtgg tggagctttt ccagttcacc
     1441 acgccgatgc ttctcttctg ccacccgggc aaacagcgcc tgtaatttca gataatcgct
     1501 ttcattcggc aactgccact gaccggcact gaaccagtgc gacaccatgt ttttctttcc
     1561 tgtggcatct gcaatctgtt ttgccgttat ccccagggca gctcgcgcat cacgaaagta
     1621 agaaatcagc ggggccatca catgctgttt cagtgcactg cccttcgccg catacccggc
     1681 atctttcgga cgatacggcc cctgataatg ttccgcgaac agaatgcgct ctgtggcggg
     1741 gaaatacgcc cgcaggcttt ccttgttgca tccgttccag cgtccggacg gcttcgccca
     1801 gataatatgg ttcagcacac tgaagcgttc acgcatcatg atttcgatat cagatgccag
     1861 gcgatgacca cagaacaggt aaagacttcc ggcaggtttc agcacccgcc agaactgcgc
     1921 cagacactgg tccagccact tcaggtaatc atcgtcgccc ttccactggt tatcccagcc
     1981 ctcaggcttc actttaaagt acggcgggtc cgtgactatc aggtcaacag aattttcggg
     2041 taacgaccgg ataaattcca ggcagtcggc gttgattaac tcacaactgg atatttttac
     2101 agtattaagc atggatcatt aagcctgtct ctgataggct cattctgctt ttgcgcaaag
     2161 cagtgggcct gaggtttgct tgtgaaccca acgcatgagc agatggctgg tgggtgcccc
     2221 taacacccac cagccgccca tttaccacaa ataaaaaagc cttcactgcg gaaggcgtct
     2281 gtaacaaccg aactgatagt ctgccagacc cgccataacc agctgggtca gtattaactg
     2341 gcagcgttcg cgtgaaaggt aagtattctg cgctatctcc ccgactgtcg ccggttcggt
     2401 aacgcttaat tcattaaaca ccactctggc ggtttctgtc atatcctgct gttttagcat
     2461 gtcttttcct tttccggtta acgtgacaca ccaataactc ttgtcgaaaa agccagcaag
     2521 ctgaaagaca ggtattcacc gccaccagcg cgtttactgt actgacgcga tttcagtcat
     2581 aaaaaacccg ctcggcggcg ggttgtagaa actcttctaa cgtcaggcat aaaacgccca
     2641 tcgttatgac gaatttacca cagattccgg aaaaatcaac cttgttacct agttaccttt
     2701 tttaactgcc gctcagccca tgcttcttca atatcaaacc gggtcaccag cgcatcata
//
LOCUS       DAZPJR010000293         2754 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPJR010000293 DAZPJR010000000
VERSION     DAZPJR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.299"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1361)
                     /locus_tag="ACHVB7_004349"
     CDS             complement(<1..1361)
                     /locus_tag="ACHVB7_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1916142.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="ACHVB7_004350"
     CDS             complement(1426..1545)
                     /locus_tag="ACHVB7_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1916143.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1769..1927)
                     /locus_tag="ACHVB7_004351"
                     /pseudo
     CDS             complement(1769..1927)
                     /locus_tag="ACHVB7_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2272..>2754)
                     /locus_tag="ACHVB7_004352"
     CDS             complement(2272..>2754)
                     /locus_tag="ACHVB7_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916144.1"
                     /translation="PEVKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGG
                     LDFKGCFFSGQNFSNYDIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLS
                     GVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ
                     "
BASE COUNT          742 a          655 c          513 g          844 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cgcctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tctccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgg
//
LOCUS       DAZPJR010000294         2739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPJR010000294 DAZPJR010000000
VERSION     DAZPJR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.300"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="ACHVB7_004353"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="ACHVB7_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR1916145.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..2374
                     /locus_tag="ACHVB7_004354"
     CDS             1322..2374
                     /locus_tag="ACHVB7_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR1916146.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          592 c          616 g          682 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat
     2161 aaaagatgct tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta
     2221 taatacccct gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga
     2281 tgtctcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc
     2341 aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg
     2401 atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc
     2461 agcctttgaa tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct
     2521 ggcggtgccg catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg
     2581 aatgtaaaaa agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga
     2641 ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt tgatcacctg
     2701 ttgccaggga aactacgtat tgttcgcttg agtttgcgg
//
LOCUS       DAZPJR010000295         2727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPJR010000295 DAZPJR010000000
VERSION     DAZPJR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.301"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHVB7_004355"
     CDS             complement(423..680)
                     /locus_tag="ACHVB7_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916147.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHVB7_004356"
     CDS             complement(677..1174)
                     /locus_tag="ACHVB7_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR1916148.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHVB7_004357"
     CDS             complement(1376..1834)
                     /locus_tag="ACHVB7_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1916149.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHVB7_004358"
     CDS             complement(1831..2328)
                     /locus_tag="ACHVB7_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1916150.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHVB7_004359"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHVB7_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR1916151.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          708 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataacc
//
LOCUS       DAZPJR010000296         2721 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPJR010000296 DAZPJR010000000
VERSION     DAZPJR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.302"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            30..2546
                     /locus_tag="ACHVB7_004360"
     CDS             30..2546
                     /locus_tag="ACHVB7_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1916152.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2721
                     /locus_tag="ACHVB7_004361"
     CDS             2557..>2721
                     /locus_tag="ACHVB7_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1916153.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          656 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga g
//
LOCUS       DAZPJR010000297         2713 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPJR010000297 DAZPJR010000000
VERSION     DAZPJR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.303"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            67..651
                     /locus_tag="ACHVB7_004362"
     CDS             67..651
                     /locus_tag="ACHVB7_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1916154.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            756..1496
                     /locus_tag="ACHVB7_004363"
     CDS             756..1496
                     /locus_tag="ACHVB7_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1916155.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1531..2713
                     /gene="htrE"
                     /locus_tag="ACHVB7_004364"
                     /pseudo
     CDS             1531..2713
                     /gene="htrE"
                     /locus_tag="ACHVB7_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          772 a          572 c          587 g          782 t
ORIGIN      
        1 ttgtgtcatg aatagtcatt cttcaaacca gtagtctgac taatggaatt ttattcaagg
       61 atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc ttatgctggc aatggttgca
      121 ggtactgctt ctgctgatat ggatggtggt cagttaaata tcagtggtct ggttgttgat
      181 aacacctgtg aaacccgcgt tgacggcggt aataaagatg gtctgatcct gctgcaaact
      241 gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca ccgttggcgc taaagccaaa
      301 cctttcagca tcaccgttga ctgcagcaaa gcaaatccga atccaggtag tacagctaaa
      361 atgaccttcg gttctgtttt ctttggtaat agcaaaggaa cgctgaacaa cgacatgtct
      421 attaacaacc cgtctgacgg cgtgaatatc gctctgcaca atattgatgg ttccactatc
      481 aaacaggttc aaattaacaa ccctggcgat gtgtatacta aagccctgga tgcaacgaca
      541 aaatctgctg tttatgattt taaagcgtct tacgttcgtg ccgtcgcaga ccaaacagca
      601 actgctggtt atgtaaaaac taacactgca tacaccatta cttatcagta agtattcacc
      661 aattaaattg attgaatgaa tatacaggga atagtgttaa taatttctat ttttatatta
      721 ttccctgttt taattaactc tgtcagggat ggtttatgtt ttttaacacc aaacatacaa
      781 cagctttatg cttcgtaacc tgtatggctt ttagttcatc gtctattgcg gacattgtca
      841 tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag tgtcaacgta cgtctggaaa
      901 ataaagggaa taacccgttg cttgtccaga gttggttaga tactggcgat aacaacgctg
      961 agcctggcag tattacagtc ccttttactg ccacgccgcc agtatcgcgt attgatgcca
     1021 aacgtgggca aacaatcaaa ttaatgtaca cagccagcac ctcactgcct aaagacagag
     1081 agagcgtgtt ctggtttaac gtactggaag ttccaccaaa accagatgca gaaaaggtcg
     1141 cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat aaaacttttc tatcgcccgg
     1201 atggattgaa gggaaatccc tctgaagccc cgttagccct gaagtggttc tggtcaggtt
     1261 cagaaggtaa ggcgtcattg cgagtgacca atccaacccc ttactacgtc tcttttagca
     1321 gtggtgattt agaagctagc ggtaaacgct atccgattga tgtgaaaatg attgcaccat
     1381 ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa agcaagttct gcaaaagtac
     1441 atttttacgc cattaatgat tttggtggcg ccattgaagg taatgccagt ctgtaatcag
     1501 gcaaggatat aattccgcag gaagcataac gtgactataa aaccaactaa aaattatcat
     1561 aatcatctga cccgtatcgc tacgttttgc gcgctgctgt attgcaatag tgctttctgt
     1621 gctgaacttg ttgaatatga ccataccttc ctgatggggc agaatgcctc aaatattgat
     1681 ctcagccggt atagtgaagg taaccctgct ataccgggta tgtacgacgt cagtgtttat
     1741 gtcaacgatc aacctattat taaccaaagc attacattta ttgaaattga aggaaaaaaa
     1801 acgcacaagc ctgtatcaca cttaagaatt tattgcagtt tcatataaat tctcccgata
     1861 taaataacga aaaagccgtt ctgcttgcca gggatgaaac actcggcaat tgccttaatt
     1921 tgacggaaat tatccctcag gcttctgttc gttatgacgt taacgagcaa cgtctggata
     1981 tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa ctatgttgat ccatcgttat
     2041 gggaaaatgg cattaatgcg gccatgttgt catacaacct caacggatat catagtgaaa
     2101 ctcctggtcg aagaaatgac agcatttatg ctgcttttaa cggtgggatg aatttaggtg
     2161 catggcgact gcgtgcctca ggcaactaca actggatgac cgattctggc agtaattatg
     2221 attttaagaa tcggtatata cagcgtgata tcgcctcgct gcgttctcaa ctcattcttg
     2281 gtgagtctta tacaacgggc gaaacctttg attctgtcag tatccgcggc attcgtttat
     2341 acagcgacag ccgcatgttg cctccgacat tagcgagttt tgcgcctatc attcatggcg
     2401 ttgctaatac caacgccaaa gtcactatta cgcaaggtgg ctataagatt tatgaaacga
     2461 ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc gtcaggttac ggcagcgatc
     2521 ttattgttac tgtcgaagaa tccgatggct caaagcggac attctcgcaa cctttttcat
     2581 cggtcgttca aatgttacgc cctggcgttg gacgttggga tattagcggc ggccaggtct
     2641 taaaagatga tattcaggat gagcctaatt tatttcaggc cagctactac tacggtctga
     2701 ataactatct aac
//
LOCUS       DAZPJR010000298         2690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPJR010000298 DAZPJR010000000
VERSION     DAZPJR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.304"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            44..769
                     /locus_tag="ACHVB7_004365"
     CDS             44..769
                     /locus_tag="ACHVB7_004365"
                     /inference="COORDINATES: protein motif:HMM:NF045398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="HFR1916156.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLF
                     QKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIY
                     RKEYATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKV
                     INESGVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACHVB7_004366"
     CDS             complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACHVB7_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR1916157.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="ACHVB7_004367"
     CDS             1604..1798
                     /locus_tag="ACHVB7_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1916158.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
     gene            2296..2457
                     /locus_tag="ACHVB7_004368"
     CDS             2296..2457
                     /locus_tag="ACHVB7_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1916159.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRL
                     EVMRLLRLN"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAZPJR010000299         2666 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPJR010000299 DAZPJR010000000
VERSION     DAZPJR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.305"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            143..652
                     /gene="eco"
                     /locus_tag="ACHVB7_004369"
     CDS             143..652
                     /gene="eco"
                     /locus_tag="ACHVB7_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR1916160.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(961..2607)
                     /gene="mqo"
                     /locus_tag="ACHVB7_004370"
     CDS             complement(961..2607)
                     /gene="mqo"
                     /locus_tag="ACHVB7_004370"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR1916161.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          632 a          737 c          663 g          634 t
ORIGIN      
        1 tgctggtttg ctccctcatc agaatttaac actaaaagag caggtaaaat tgtctgaatg
       61 ttctttaagt tattcataaa gcaaattaat aaatctgatg aatatgttaa ccttcagcga
      121 catcatcggt gaaaacctat aaatgaagaa ggaaagcaaa aaaatgaaga ccattctacc
      181 tgcagtattg tttgccgctt tcgccaccac ttccgcctgg gcggcagaaa gcgtccagcc
      241 actggaaaaa atcgcgcctt atccacaagc tgaaaaaggg atgaagcgtc aggtgattca
      301 gttaaccccg caagaagatg aatctaccct gaaagtagaa ctgttaaccg gtcagacgct
      361 ggaagtcgat tgcaatttgc atcgtctcgg cgggaagctg gaaaacaaaa cgctggaagg
      421 ctggggctat gattattatg tctttgataa agttagctcg ccggtttcca cgatgatggc
      481 ctgcccggat ggcaagaaag agaagaaatt tgtcaccgcg tatctgggcg atgctggaat
      541 gctgcgttac aacagcaagc tgccgatcgt ggtgtatacg ccagacaatg tagatgtgaa
      601 gtaccgcgtc tggaaggcgg aagagaaaat tgacaacgcg gtagttcgct aaactgctgt
      661 gaagtgcgtc accccgtagg tcggataagg cggtcacgcc gcatccgaca tctaacgccc
      721 aagccggttg cctgatgcga cgctggcgcg tcttatcagg cctacaccgc tgtgaagtgc
      781 tccaccccgt aggttggata aggcggttac gccgcatccg acatctaacg cccgagccgg
      841 ttgcctgatg cgacgctgac gcgtcttatc aggcctacac cgctgtgaag tgctccaccc
      901 cgtaggtcgg ataaggcggt tacgccacat ccggcatacc atgccggatg tggcacatca
      961 ttacaacgca atatccgcca cttctttttg aacgggttgc ggtttcaact gcggtttcgg
     1021 cgtactatct gctgcctgcg gcttgtcgta tttcaggccc agcacttcgc tggtgtactg
     1081 caactcgcgt tctgttgccg ctacatcacc gttcagcttg cgtccataag atggaacgat
     1141 cgctttcaac gtagcctgcc attgcgggct ggaaacgcga tcgccaaata ctttttccag
     1201 cagatccaac ataatcggcg cagcggttga cgcccctggc gatgccccca gcagcgcggc
     1261 aatggtccct tgctggtcac tgaccacttc agtacccaga cgcagtacac cacctttatc
     1321 ggcatcacgc ttgataatct gcacacgctg ccccgcttgc cacaaacgcc agtcctcttt
     1381 tttcgcctgc ggatagtact ctttcaacgc ttcaaaacga tcctcttcac tcaacatcac
     1441 ctgactcacc aagtatttca ccagatcgaa attatccagc ccgacgtgca tcatcggcat
     1501 cacgttagag gtggtggtgg aactcatcag atcccacaat gaaccgtttt tgaggaattt
     1561 ggttgagaag gtggcaaatg gcccaaacag cactacgcgt ttaccgtcca gaacgcgggt
     1621 atcgatatgc ggaaccgaca tcggtggtgc gccaacggat gctttaccgt aaaccttcgc
     1681 cagatggtga ttaaccacgt ccgggttttc cgaaacaagg aactgtccgc ccaccgggaa
     1741 accggcgtag tctttcgctt ccggaatccc cgattcctgt aacagcttca gcgccgcacc
     1801 gcccgcgccg ataaagacaa atttggcacg aatgttctgt gcagtgccat ttttcagatc
     1861 ggcaacggta acggtccagg tgttatcgtc attacgcttt agggcgcgga cttcgctgct
     1921 gagttgcagc gagaagttag atttcttctg caaggaagca attaactggc gggtgatctc
     1981 gccgtagttc acatcggtac caatttccgt acgcgtggct gccactttct gttgcggatc
     2041 gcgcccttcc atcactaacg gtgcccactc tttgatctgc gcgtgatctt cagagtaacg
     2101 cataccgcga aacagcgagc tttgttgcaa cgcggcgtaa cgggcgcgca ggaaattaac
     2161 gttatcctcg ccccagacaa agctcatatg cggaacggta ttgataaatg aacgcggagt
     2221 acgcagcacg ccgcgctcga cctggtgcgc ccagaactgg cgggaaatct gaaatgcttc
     2281 gttaatggcg actgcttttt caatactgat actgccatcg gcgttttgcg gggtgtagtt
     2341 cagttccatc agtgcagaat gcccggttcc ggcgttgttc cagccgttcg aactctcctg
     2401 cgcgacaccc tccaggcgct ccaccatggt catcgaccat tcaggctcca gctcgcgtaa
     2461 ataggtcccc aacgtggcgc tcataatgcc gccgccaatc aacagtacat cagtttcctg
     2521 ctcctcggac gctttcgctt ttgccgccat cgaaacggca ttaagcccca cggccatcga
     2581 gaagagcatg gcagtcactt ttttcatctt gttaatgcct tacttttagt cgctttattg
     2641 caggtgagat ttgcgcggca tcaacg
//
LOCUS       DAZPJR010000300         2652 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPJR010000300 DAZPJR010000000
VERSION     DAZPJR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.306"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHVB7_004371"
     CDS             <1..122
                     /locus_tag="ACHVB7_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916162.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHVB7_004372"
     CDS             119..376
                     /locus_tag="ACHVB7_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916163.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHVB7_004373"
     CDS             376..750
                     /locus_tag="ACHVB7_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1916164.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..1601
                     /locus_tag="ACHVB7_004374"
     CDS             747..1601
                     /locus_tag="ACHVB7_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1916165.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTRLIF
                     PESVVNSS"
     gene            1704..1779
                     /locus_tag="ACHVB7_004375"
     tRNA            1704..1779
                     /locus_tag="ACHVB7_004375"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1737..1739,aa:Ile,seq:cat)
     gene            1880..1956
                     /locus_tag="ACHVB7_004376"
     tRNA            1880..1956
                     /locus_tag="ACHVB7_004376"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1914..1916,aa:Arg,seq:tct)
     gene            2456..2587
                     /locus_tag="ACHVB7_004377"
     CDS             2456..2587
                     /locus_tag="ACHVB7_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR1916166.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
BASE COUNT          701 a          530 c          732 g          689 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaaggt ccagtatccg gatggtaagc cgccggtatg ggcaaagtgt acgaaaggtg
     1201 tgtatccgtc ttactgggaa gaatggaaaa aagtcaggga ggtggtaaaa gttgcctgtc
     1261 cggagtgtgg cggaaagggt gaggtttcca ccgcctgtaa ggattgccgt gggcgtggtg
     1321 tcgccattca tcgtgaagag tcggtaaaac gtggtatgcc tgttatcaga gactgccagc
     1381 gttgtggtgg tcgtggctat gaaagactac catcaacgga ggcatttaat gctatatgcg
     1441 aggtgacaaa ccagataaca cgcgcgtcat gggaaaaaac agttaagaaa ttctatgatg
     1501 cgctggtgac tcggtttgat attgaagaag catgggctga gcggcagtta aaaaaggtaa
     1561 ctaggttgat ttttccggaa tctgtggtaa attcgtcata actatgggcg ttttatgcct
     1621 gacgttagaa gagtttctac aacccgccgc cgagcgggtt ttttattgcg gaattaatta
     1681 cggaccgtta ttattctgct cccggccctt tagctcagtg gtgagagcga gcgactcata
     1741 atcgccaagt cgctggttca aatccagcaa gggccaccat cacaaaccgc cattagctta
     1801 tcaggaagag cagacgacac gataacaggg ttgttggtgc gggggcgggt ccccgatggc
     1861 ggtccattat cggtattcag cgttgttagc tcagccggac agagcaattg ccttctaagc
     1921 aatcggtcac tggttcgaat ccagtacagc gcgccatatt cattcttcca gattccttcc
     1981 ggcagagcct tatactgaaa tatacctggc tcaggatatt gttgaaaata ttttatgttt
     2041 gtcaaaaata aaagttctgt taagtattga ttgagtgttt gttatacggt ctaatggttt
     2101 tttcagcatt aaatatttat cattcatatg gtgtgggtag agtgaatatt gatgaggcgt
     2161 cggggtgttt catccttagg cagcgtattg atatagtcaa tgcagcacga gcaaaggcct
     2221 tcagccgttt gacagttttg ttctgtactc ctgatcgtct ttcgggaaga gacgttatta
     2281 ttctgaatag tgatgctata cagagggttt gcgatgagtt catggttgct aattcagaat
     2341 tatttgctct tgttcaggag tacaacagaa tagccaggac ctgtggtatg gatgaacttc
     2401 ggattactca tctggggtag atacatatct ggattatcac ctgttacggt aaaaagtgat
     2461 tgcttactgt ttttgtgaat ggcattgcag cagccggata atgtcagtgc tggctgacgg
     2521 tgtgctggtg gcgggtgtgg tggttgttgc tttcccgttg ctgaaaaaga aaacgccaga
     2581 ctgttagccg ggtatcagtt agcgggagaa atttttaaat acttcacaat tcaggcggtt
     2641 gactgttgtc tg
//
LOCUS       DAZPJR010000301         2644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPJR010000301 DAZPJR010000000
VERSION     DAZPJR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.307"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            12..107
                     /locus_tag="ACHVB7_004378"
                     /pseudo
     CDS             12..107
                     /locus_tag="ACHVB7_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            403..1398
                     /gene="pgl"
                     /locus_tag="ACHVB7_004379"
     CDS             403..1398
                     /gene="pgl"
                     /locus_tag="ACHVB7_004379"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1916167.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1439..2392)
                     /locus_tag="ACHVB7_004380"
     CDS             complement(1439..2392)
                     /locus_tag="ACHVB7_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1916168.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          673 a          588 c          648 g          735 t
ORIGIN      
        1 ggggatttac catgcttatt ggctatgtac gcgtatcaac aaatgaccag aataccgatc
       61 tacaacgtaa tgcgtttatt tgtgccggaa gtgagttgat tgttgagcga atgggaagtt
      121 ggccagaagt tgttatggca tagtgtttac ggtcgttgat acaggtaaac aaaagcgggg
      181 agagtcatac tccctgaata gtcgcgaaaa tatctgtggc attgtcaaat tcgtgtcggt
      241 gactaatatt ttagtaatat gccattcgaa atggagtgct aacttattga tttaattgat
      301 gtgcgatatt gtattttatc gtgttttgtt gtctgttttt tatccagtaa gtgttgttat
      361 gtgtatgaaa gataagggat ttatcatttt tgaggtgtgt tcatgaagca aacagtttat
      421 atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga aggcgcactg
      481 acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt cagcccggac
      541 aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta tcgtattgcc
      601 ccggacgatg gcgcactgac ctttgccgca gagtctgcgc tgccgggtag tccgacgcat
      661 atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg
      721 agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg
      781 gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt tccggcatta
      841 aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt ggcgcaggac
      901 cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac
      961 gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg ggaactgaaa
     1021 gatccacacg gcaatattga atgtgtccag acgctggata tgatgccgga aaacttctcc
     1081 gacacctgtt gggcggctga tattcatatc accccggatg gtcgtcattt atacgcctgc
     1141 gaccgtaccg ccagtctgat taccgttttc agcgtttcgg aagatggcag cgtgttgagt
     1201 aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga tcacagcggc
     1261 aagtatctga ttgtcgccgg gcaaaaatct caccacatct cggtatacga aattgttggc
     1321 gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc aatgtgggtg
     1381 gtggttaacg cacactaacc gctgatttac ccggcgcagt ctctcctgcg ccggtgtatt
     1441 aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac aaattgtctt
     1501 tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttctgctgcc ggagaaagca
     1561 cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc accagtggaa
     1621 taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc gccgaaatcc
     1681 ccagtcccgc ctctaccagt cctaatgacg tcgaaagatg acgcacttcg taaaaccagt
     1741 ccagcttcca gggcttgtcg gccagctgtt gttctatcaa cagtcggttg ccgctggagg
     1801 agcgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc tgctttttgg
     1861 ccaatggatg gtcacgccga caggccagca cgaacggctc gttgaccagt ggggtaaaat
     1921 caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg ttacacagca
     1981 ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata ttgggataca
     2041 gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc ggaatacacg
     2101 ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa agcgtttcat
     2161 cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct tttgttaacg
     2221 taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc atctttttta
     2281 ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg ttaaacgaac
     2341 tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc attattactc
     2401 cggaaaatgg aagcgacgat tttgggtggc tggccgttaa aaattttaac tgcatttagc
     2461 caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta gagatgcttt
     2521 gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca ttattatgaa
     2581 aaaaataccc tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc
     2641 gatg
//
LOCUS       DAZPJR010000302         2643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPJR010000302 DAZPJR010000000
VERSION     DAZPJR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.308"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            439..582
                     /locus_tag="ACHVB7_004381"
     CDS             439..582
                     /locus_tag="ACHVB7_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR1916169.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            595..804
                     /gene="cspE"
                     /locus_tag="ACHVB7_004382"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             595..804
                     /gene="cspE"
                     /locus_tag="ACHVB7_004382"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1916170.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            970..1779
                     /gene="rlmA"
                     /locus_tag="ACHVB7_004383"
     CDS             970..1779
                     /gene="rlmA"
                     /locus_tag="ACHVB7_004383"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR1916171.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1776..2342)
                     /gene="mntP"
                     /locus_tag="ACHVB7_004384"
     CDS             complement(1776..2342)
                     /gene="mntP"
                     /locus_tag="ACHVB7_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR1916172.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          699 a          611 c          626 g          707 t
ORIGIN      
        1 gtttctctct tgattttttt gctcattcat caatttttct tattttaaat tttcaatccc
       61 tttggggatt gacttctctt tagggtaatt gatagccgtt aaccgactgt tttatgagaa
      121 aaagtgatat aactttttat tcattgcata gcaaaaaatg tgatattgca cgcactatgt
      181 aataacttct cccactggcc tggaacaact gaacttattg aactatgtta gaaaatacgc
      241 cagtttaagt atctgcctga actggcaagg ttaagcacaa tgatatatcg gcgcgtattc
      301 cgttgcataa gtgtgcaaaa aaagtggaag acgtatcgag atttgtgcgt ctgatcgaga
      361 catgtttaaa aatggcttgc cataattaac gttgtatgtg ataacagatt tcgggttaaa
      421 cgaggtacag ttctgtttat gtgtggcatt ttcagtaaag aagtcctgag taaacacgtt
      481 gacgttgaat accgcttctc tgccgagcct tatattggtg cctcatgcag taatgtgtca
      541 gttttatcta tgttatgcct gcgggcgaag aaaacaatct aaggaatttt tcaaatggca
      601 aagattaaag gtcaggttaa gtggttcaac gagtctaaag gttttggctt cattactccg
      661 gctgatggca gcaaagacgt gttcgtacac ttctccgcta tccagggtaa tggcttcaaa
      721 actctggctg aaggtcagaa cgttgagttc gaaattcagg acggccagaa aggtccggca
      781 gctgttaacg taacagctat ctgatcgaat ccactgatct gaagtgtgaa tacgcttcaa
      841 tctcgctata aagcctcgtc gaatgcgagg ctttttacta tgctttatct tcgctcctgg
      901 cgttcggata tttgcccgcc gcgtgattcg cgttacactt gcggccttta gtatcctgcc
      961 ggagttgtca tgtctttttc ctgtccactt tgccatcagc ctctttcgcg tgaaaaaaac
     1021 agctatatct gtccccagcg acatcagttt gatatggcga aagaagggta tgtcaatctg
     1081 ctgtccgttc agcataaacg gtctcgtgat ccgggcgaca gcgcggaaat gatgcaagca
     1141 cgccgcgcat tcttagatgc cggacattat cagccgctgc gtgatgcaat tgtcgcccaa
     1201 ctgagggaac ggcttgatga taaggccacg gcggtgctgg atattggctg tggtgaaggg
     1261 tattacacac acgcatttgc cgatgcgttg cccgaaatca ccacgtttgg tctggatgtt
     1321 tcgaaggtag cgataaaagc ggcggcgaaa cgctatccgc aggtcacttt ttgtgtcgct
     1381 tccagccacc gtttgccgtt ttccgatacc agtatggacg ccataatacg tatttacgcg
     1441 ccgtgtaaag cagaagaatt agcacgagta gtgaagcccg gcggctgggt cattactgcc
     1501 acgccgggac cgcgacattt gatggagctg aaggggctga tttacaatga agtacatctt
     1561 catgcacctc atgcagaaca actggaaggt tttacattac agcagagtgc ggagttgtgt
     1621 tatccgatgc gtcttcgcgg tgatgaagcc gtcgcattat tgcagatgac gccgtttacc
     1681 tggcgtgcga agccagaagt ctggcaaaca ctggcagcaa aagaagtgtt cgactgccag
     1741 acggacttta atattcacct ctggcagcgt tcttattaac cgtggaagtg cgtccagagg
     1801 atctggacgc cgatgccgat cagtaccagc ccgccgagaa tttctgcttt tttcccaata
     1861 attgagccga taaagcgacc aaccatcatc cctaatgttg acataatcaa ggttgcacaa
     1921 ccaatggcca atgcggtcgc gataatgttg acctgcagga aagcaagacc aacacccaca
     1981 gccatggcat ccaggctggt ggcaatcgcg gtggttacca gtagccagaa accgtgtcga
     2041 cggcgcggct cttcatcttc atcatctgcg ccacgaaaac cctcaataat cattcgcccg
     2101 ccgaggaata tcagcagcac aaacgcaatc cagtggttcc attcaaggac aaaccggctg
     2161 gctaacatgc ccattcccca gccgatcagc ggcgtcaggg tttcgacggc accaaaaata
     2221 aggccggttc gcaatgcttc agaaaatttc ggtttatgga gggtggcacc tttaccgatt
     2281 gatgcagcaa atgcatccat cgacatacca aacgcaagaa gaacagtagc agtgatattc
     2341 atgacaatgt cctgaccggg gtatccataa aacacatcat cgcccccagc aaacatgcat
     2401 acatgaacaa caagccgtga tggttttcgc gttcatgcag gcatagcgat tgatgtgcat
     2461 atggtctcgc ctgactgaaa gtattcagtc cgtacgtgcc acgttttgca acgagtatgt
     2521 tgacacgtac atttccggaa tctggaaatc ggctactccc caaatgaggg cgcaacctta
     2581 acatattttg agaatataaa acaactgttg gaatatatta tttcattgct ttattgataa
     2641 cga
//
LOCUS       DAZPJR010000303         2569 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPJR010000303 DAZPJR010000000
VERSION     DAZPJR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.309"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..344)
                     /gene="quuD"
                     /locus_tag="ACHVB7_004385"
     CDS             complement(<1..344)
                     /gene="quuD"
                     /locus_tag="ACHVB7_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR1916173.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALD"
     gene            complement(430..570)
                     /locus_tag="ACHVB7_004386"
     CDS             complement(430..570)
                     /locus_tag="ACHVB7_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR1916174.1"
                     /translation="MMFEFYMAEFLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(567..929)
                     /gene="rusA"
                     /locus_tag="ACHVB7_004387"
     CDS             complement(567..929)
                     /gene="rusA"
                     /locus_tag="ACHVB7_004387"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR1916175.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(926..1216)
                     /locus_tag="ACHVB7_004388"
     CDS             complement(926..1216)
                     /locus_tag="ACHVB7_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1916176.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(1209..1379)
                     /locus_tag="ACHVB7_004389"
     CDS             complement(1209..1379)
                     /locus_tag="ACHVB7_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR1916177.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(1379..1834)
                     /locus_tag="ACHVB7_004390"
     CDS             complement(1379..1834)
                     /locus_tag="ACHVB7_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR1916178.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            2099..2476
                     /locus_tag="ACHVB7_004391"
     CDS             2099..2476
                     /locus_tag="ACHVB7_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916179.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT          688 a          617 c          586 g          678 t
ORIGIN      
        1 atatctaatg ccattaacat cccttcaatt atgccctcag ccttctgtaa ccttttcccg
       61 atataaccat cagagcagca atgcttacct gccagtgaca tgaatgtcat accgactaca
      121 taataatcta ctaataaatc gtgcaaatcg ctgttgttct ttttcagacg ggccatgcac
      181 ccgcaaataa tcatcgcgtc atcgtcacaa cattgcgggc gagattttac ttttgaagta
      241 attaatccct taaaaccggc ggcaatggac gaccaggtca catcttcatg attattagcc
      301 gcccacgctc cccaacgctc aagaaccatc tgaatatcac gcatcaactt actccacaaa
      361 aatcagacca gaacgccaat tacaagcaaa aatcaacaaa acagtattag ttgattgtta
      421 tctctgactt catactcctg ctcctgtcag ggttttggcg taattcttca gtattcggta
      481 atcggtcaaa acagaaccgg ggaaacgata taagcgcaga tgcccccagc ggtggcgaag
      541 aaattctgcc atataaaact caaacatcat tcattcccca tttcggtgat ggtcagttcc
      601 agcctcccac ctttggtaac aggcatcttc acaacgcggt aatcaacgac ctgagcatca
      661 tccagccaga aacctgcttt ggtgagtgcg tcaaaagcgg ctttttgcag attatccagg
      721 tcacggcgac ggcgatccgg catgtggcac tcaatacgga ttttcactgg catagccagg
      781 ccgatatcca gcattgcgtt tttaatgatt cgggtgacgt tatcgcggta tgcctgccct
      841 tctgcgctga tgtgtgtgcg cccgcgatta tggcggtaat agcggttatt gctcggaggc
      901 cagggtaatg tgatgctgta ggtattcacg ccttaataac cccctctttc agccagataa
      961 cctgcgttct cgccatacct tccagcgcgc attcttttgc atactcagca tcgacaaaat
     1021 atgtgcggcg gtcgatttcg tcgtgacagg cagaacatgc aatggtggca atcaggtctg
     1081 gcggtttgat accggtaccg cacaatccag ccagccggat atgtgccagt actgacgttt
     1141 caggattgcc attacatacg ccagggattc ttacctggca ttcccgacca cgcgctgctt
     1201 ttctcaaatc agccatgatt cctccttgct gccagtcgca accatttttt atcaaccagg
     1261 ctagcggtat atccgagcag tgttggtatt tcggatggct tcagctcagg cttacgctta
     1321 cgacgatttg atactctgta gatgtgcccg ttcatgacac gaataagcgg tgtagccatt
     1381 acgcctcctg cttatcgcgc agctgctggt attcacagct ctgcggaatt gtcaggtggc
     1441 agccgatatt catcgcccag gcttcgactt tgcacaggaa gatgtacatc tcgccggttt
     1501 ccagctctga cgtatggcgg agggattgga cggtggtgac ctcgccggac acgacatcta
     1561 cacggtcttt gctttcgtag ccgagatagg tgtgcttcat cgcgtctttg acccactcag
     1621 gcgtagcgaa ggtcttgccg cgggcgatga gatattcgct gatctccgtg taccacatgt
     1681 ggctgagcgc gttctgcgac aggctgcgct tctcgcgcca cggcttaacc tgcaggcgta
     1741 agcattgccc ggcatccagc aatggctgaa tctgctggcc gatggccgcg aagttaccgc
     1801 gatggagttt gatgccgtct actggcagag tcatacggcc tccctaacgg aaaccgcaga
     1861 atgcagaaaa tcgcaggtgc atttctgcat ctgtgacaag gtgatgagtt cagattgtgg
     1921 tcgcatttaa gtccccttaa atgcgcagaa gtcaccggag ttgttcaggc tccgatgaga
     1981 ggattatggc ggattgatta cgataaatca aaactatctt gaagccaaaa cagtgatctt
     2041 cgctttaaaa accttgtata ctttcaccta atcacagcca gaactatgga tctaaccaat
     2101 gaccaacaaa gaaaacaaag ataaatatat taacaagttc aagaaagagc tttcaaaata
     2161 tatacaacca actgctggag tggacattca attatttaac tctaaggatg gtggtggtgt
     2221 aattaaagcc actttgaata gaagtggaaa aagaaaatca tctattgctg gtaattttac
     2281 taaattaggt gaggcaatta cctcgagtgg gcagagggtt tttggtggtg atctatcaaa
     2341 tgtgaatttt tacggaacaa atacgatttt tgatggagat acaatatttt taattaaatc
     2401 acctgattca gaggagtggt catcacaaaa agcttccaaa gacgtagagg gcatcgtatt
     2461 tggagggaaa aaatgattaa cattactact aaggatgcac tcaagtcagt ggtgaagtaa
     2521 gagatctagt aattcttctt gaaactgatg gaaaaacggt gtctgatta
//
LOCUS       DAZPJR010000304         2556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPJR010000304 DAZPJR010000000
VERSION     DAZPJR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.310"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHVB7_004392"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHVB7_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR1916180.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHVB7_004393"
     CDS             complement(390..1178)
                     /locus_tag="ACHVB7_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR1916181.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHVB7_004394"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHVB7_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR1916182.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHVB7_004395"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHVB7_004395"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1916183.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHVB7_004396"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHVB7_004396"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR1916184.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          665 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgt attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAZPJR010000305         2483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPJR010000305 DAZPJR010000000
VERSION     DAZPJR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.311"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            83..2350
                     /gene="adiA"
                     /locus_tag="ACHVB7_004397"
     CDS             83..2350
                     /gene="adiA"
                     /locus_tag="ACHVB7_004397"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR1916185.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          623 a          682 c          613 g          565 t
ORIGIN      
        1 gtttttcacg cgctttacag cccgaaaagg ccggaagata cttgcccgca acgaagattc
       61 cttcataacc gggtaagcaa tgatgaaagt attaattgtt gaaagcgagt ttctccatca
      121 agacacctgg gtcggtaacg ccgttgagcg tctggcagat gctttaagcc agcaaaatgt
      181 taccgtgatt aaatccacct cctttgatga tggttttgcc attctctctt caaacgaagc
      241 cattgactgc ctgatgttca gctatcaaat ggaacatccg gacgaacatc aaaacgtcag
      301 acaattgatc ggtaagcttc atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga
      361 tcgggaaaaa gccctcgccg caatggatcg cgacctgctg gagcttgtcg atgaattcgc
      421 ctggattctg gaagataccg ccgactttat cgccggacgc gctgttgccg cgatgacccg
      481 ctaccgccag cagctgttgc cgccactgtt cagcgcgctg atgaaatata gcgacatcca
      541 tgaatattcc tgggcagcgc caggccacca gggcggcgtt ggttttacca aaacacccgc
      601 cggacgtttc taccatgact actatggtga aaatctgttc cgcaccgata tgggcatcga
      661 acgaacttcc ctcggttctt tgcttgacca tactggcgca tttggcgaaa gcgaaaaata
      721 tgccgcacgc gtatttggtg ccgatcgctc ctggtcggta gtcgtcggta cttccggctc
      781 taaccgcacc atcatgcagg cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa
      841 ctgccataaa tccatcgaac aaggcttgat gctgacaggc gcgaaaccgg tctacatggt
      901 gccaagccgc aaccgctacg gcattatcgg gccaatctat ccgcaggaaa tgcaacctga
      961 aaccttgcag aagaaaatca gtgaaagccc gctgactaaa gacaaagccg ggcaaaaacc
     1021 gtcttactgc gtggtgacca actgcaccta tgacggcgtg tgttataacg ctaaagaagc
     1081 gcaggatctg ctggaaaaaa cctccgatcg tctgcacttt gacgaagcct ggtacggcta
     1141 tgcacgtttc aacccgatct atgccgatca ctatgccatg cgcggcgaac ctggcgatca
     1201 caacggtcct accgttttcg ccacccactc cacccacaaa ctgctgaatg cgctttcaca
     1261 ggcttcttat attcatgtac gtgaaggtcg tggggcgatt aacttctccc gcttcaacca
     1321 ggcctacatg atgcatgcca ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt
     1381 ggcggtgtcg atgatggacg gcaacagcgg cctgtcactg acgcaggaag tgatcgacga
     1441 agcggttgat ttccgtcagg cgatggcgcg gctgtataaa gagttcaccg ctgacggtag
     1501 ctggttcttc aaaccgtgga acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta
     1561 tgactttgct gacgcaccaa ccaaactgct gaccaccgtt caggactgct gggtaatgca
     1621 tccgggcgaa agctggcacg gcttcaaaga tattccggat aactggagta tgctcgaccc
     1681 gattaaagtc agcatccttg ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg
     1741 tgttccggcg gcgctggtca ctgcctggct tggtcgccac ggcattgtgc ctacccgcac
     1801 cactgacttc caaattatgt tcctgttctc tatgggcgta acccgtggga aatggggaac
     1861 tctggttaac accctttgct ccttcaaacg ccactatgac gccaacacac cgctggcgca
     1921 ggtgatgccg gaacttgttg aacaatatcc tgacacttac gcgaacatgg ggattcacga
     1981 tctgggtgac accatgtttg cctggctgaa agaaaacaac cctggcgcac ggttgaacga
     2041 agcctattcc ggcctgtcgg tggcggaaat caccccgcgt gaagcgtaca acgcgattgt
     2101 cgacaacaat gtcgaactgg tatccattga aaatctgcca ggacgcatcg cggcaaactc
     2161 agttatccca tatccgccag gaatcccgat gctgctgtct ggtgaaaact tcggcgataa
     2221 aaacagtccg caagtaagtt atttacgctc gctgcaatcc tgggaccacc atttccctgg
     2281 atttgaacac gaaactgaag ggactgaaat tattgacggt atttaccacg ttatgtgtgt
     2341 gaaagcgtaa ccactattcc gctgaaggcg taattgttta aataacatta cgccgcctgg
     2401 ccttaggcct tttgagtatg gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat
     2461 actttttgcg cggccccacc ccg
//
LOCUS       DAZPJR010000306         2472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPJR010000306 DAZPJR010000000
VERSION     DAZPJR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.312"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(1..713)
                     /locus_tag="ACHVB7_004398"
                     /pseudo
     CDS             complement(1..713)
                     /locus_tag="ACHVB7_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(766..1362)
                     /locus_tag="ACHVB7_004399"
     CDS             complement(766..1362)
                     /locus_tag="ACHVB7_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR1916186.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="ACHVB7_004400"
     CDS             complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="ACHVB7_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR1916187.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2006..>2472)
                     /locus_tag="ACHVB7_004401"
     CDS             complement(2006..>2472)
                     /locus_tag="ACHVB7_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1916188.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          679 a          537 c          496 g          760 t
ORIGIN      
        1 tggcctgcgc cataggcttt aaatgaatac agataatttg atgagctgga taatttgatc
       61 tgttgagtta ctgttggatc aaagtttgta tctgtataga attcaattgt aagggtctga
      121 taaataccac ctacagccca gtatttacgg gtgctattcg cattattgca actccaatat
      181 aacacgtctt cgcttggatt ataccacgaa aaatattgtt ctgcagagtc accaatgtat
      241 atcccaggtg aactaacgtt agttacggta ccgtaagctg accacatgtt tgaaattaat
      301 aatgtgtaat acaaaccagg aactgatgta ttaaataatt tatgggagcc atacatttta
      361 cctgagtcaa ccattgcatt ttcaatacgc attgcgctac cacttgtgct atcacttctg
      421 ttgcaataaa gaaaaccacc accagaagcg gtatctaact gactctactg taattttaca
      481 cctgtaggtc caagctgcgt cggtttgtag aaaactaaat tatttgtact tgttgcatat
      541 ctgaatatga cagtagcatc gccattcggc cccgtttctc tccatttttg atctgacgga
      601 ccaacgccgt aactattccc aacaatatat cctacgttat catcagtgcc agcactcgcg
      661 gtataacaac tcaagcacag aattaaaaaa aaatgtctga agaatttctt cattatcact
      721 ccaccatcta ctgctgtaac agtcgcagtt atttgttttg atctattatt cgtagtacac
      781 atcaactaat atatgactca ttacttggcc agatatgaca tccagcgcgg gatcgatttt
      841 ttgcattctg gcataaaatt tccatgaaga attatgcagg gcagaagtca ttactggata
      901 cacggaacga gaatttcctg ctgaactaat aacattgaat atattggtcg ggtcctgaat
      961 ggaaaagaga acgacaccga catttttagc cccggttgcg acttcattgg caaatatttg
     1021 ccttgaacct gcagccatga caccgctaag ggctctaaaa tcaatatgta actgattaag
     1081 cacatcgcca gttccctgta atgcacagtc tgatactgtg acagtgaact cttttccacc
     1141 tggttgatag ctttcaggag taacattatc tgaaaaatat cccaccccaa cgacgccaag
     1201 atcaatatta ccctggttac tgattccgct tttgcaagtg ctgtttttta cattggcaac
     1261 aaggtcaata tcctgcccag cgatggctgg ataggtaatg ggaaactgta gtgacagaaa
     1321 cagaattatt ctcttcatca gcttacgttg agtgggatgc atatatagtt cctggatctg
     1381 tcagtctgtt agttataggt tactgtaaat gtggcaggtg cagaaaattt acccgcagtg
     1441 acattgctta cggttttatt tttttcgacg accagtcgag cactcatcgg ataataaaca
     1501 accgcgttgg ctgcattgga gatagtgacc tcttgagatg ctggtgtcac acaactaaat
     1561 tgtgttgcgc tccctgttgc cggagtttcg gtgctccaga cttcaacggc gacggcactt
     1621 gctttatctg cggctgtgga tccatttgcg agccccgcac cgtcattagc agtaccttcg
     1681 catgtggctc gcttggttaa ttttatttgt gcttttttat tggggatacc cgcacagtct
     1741 ttgaatttga gtttgaatgt tttaactttg gtcttggcat tgatttcaga aatatataca
     1801 tcgccaaagt caacaacaga aatgtcctga tcagagtcat ttactaatgt agcggtacaa
     1861 gtacctattt ctacggtagt aatcacggtc agcccaatgc tgtctgttgc cgcataagag
     1921 ggtacgctga acaatgaggc agccaaaacg acactgctgg ataatccata acgtaaattt
     1981 ttaaatgtca tcttaattat ccttcttatt gatagatgaa attaaacgtt gccagtgcac
     2041 gaaaattccc cggtacgcct tgccctgcat ctgtttcacg tagcgcaacg gtcatttcaa
     2101 gacccttatc gggctgcatc tcgtctgtgc tccagcgtat cttttccgca ctgttaggtt
     2161 taaggaaagt ggcatcatca gtagtccgtt ttttgaaacc cataccgata ttacttgtcg
     2221 tcgaagatga atcaccagac tgcggtataa taagcttagg tgagcttgat gatgcatttc
     2281 cggtcagagt ggtatcaatc caactgatgc cactgctgca cccactagca accagtttaa
     2341 aatcagcctg agattctgtc gttttattcg cgatcttatc cagacccatc ttagggattc
     2401 tcaatgtgta gttattattg ccatcattcg tgacgttatt accagtaagt gtgatgttac
     2461 aggttgtcgc tt
//
LOCUS       DAZPJR010000307         2464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPJR010000307 DAZPJR010000000
VERSION     DAZPJR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.313"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(107..1525)
                     /gene="phrB"
                     /locus_tag="ACHVB7_004402"
                     /pseudo
     CDS             complement(107..1525)
                     /gene="phrB"
                     /locus_tag="ACHVB7_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
     gene            complement(1522..2031)
                     /locus_tag="ACHVB7_004403"
     CDS             complement(1522..2031)
                     /locus_tag="ACHVB7_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR1916189.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          628 a          632 c          565 g          639 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
      601 tctgccactg tacacgatcc gtctaggcaa taattggacg atgtttacac aacgaggggt
      661 gatacgttat caggtgacgg taaaactcgc gccagatcag ctcattaagc cagacactac
      721 cgaccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
      781 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
      841 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
      901 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
      961 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
     1021 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
     1081 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
     1141 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
     1201 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
     1261 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
     1321 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
     1381 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
     1441 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcttggc
     1501 gaaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
     1561 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca tgataaagcg ccatatagtg
     1621 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
     1681 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
     1741 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
     1801 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
     1861 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
     1921 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
     1981 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
     2041 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
     2101 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagtga gaattattct
     2161 actatttccc gagctgcgtt cattttttgt attttttatt tttagaaaat aacagttctc
     2221 aaagcttaat tacctcacta ttaattgtat ttcaaactaa caatcgcact ccattttctc
     2281 tttttaaaaa acataaacac tgttaaaaca aatacttatt tcgttatctt aaatgctatt
     2341 tctacatcac tcgtccttat aatcaaatta ttcttattca tagctatttt tgcaggttta
     2401 aaatagcggc tttcaccaaa gtcagccatg aaaattagac tacaagtcag ggcaagatta
     2461 cgaa
//
LOCUS       DAZPJR010000308         2455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPJR010000308 DAZPJR010000000
VERSION     DAZPJR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.314"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..989)
                     /locus_tag="ACHVB7_004404"
     CDS             complement(<1..989)
                     /locus_tag="ACHVB7_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1916190.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIK"
     gene            complement(1015..1707)
                     /gene="rfaY"
                     /locus_tag="ACHVB7_004405"
     CDS             complement(1015..1707)
                     /gene="rfaY"
                     /locus_tag="ACHVB7_004405"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR1916191.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            complement(1717..>2455)
                     /locus_tag="ACHVB7_004406"
     CDS             complement(1717..>2455)
                     /locus_tag="ACHVB7_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1916192.1"
                     /translation="DPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVC
                     KGSLKPLTEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYVNIKKWHE
                     ANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSH
                     RKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEM
                     QKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          760 a          490 c          429 g          776 t
ORIGIN      
        1 aatttaataa agtaaataaa agcgttaaac gtatcaccat ttttctttgc atgttgatat
       61 ttcacttgat actgcttttc attcgtggct ggaataaacg ccacatcatt ccagcaagaa
      121 gcctgataag cctcattaaa atacttctgg cagggataat tcgtccagtc gttccagggc
      181 ttcgttacgc caacataatg aatcagctta gtatcatcgg taatggtctt tttataatct
      241 tcatcgcttt tattttttaa ctcataatcg atgccataaa agcaatcata atcgccgcta
      301 ataaaaatac aatgaccaaa gaacagaata tttaaaatat cctgatcaag gcatttatac
      361 ttcgtgtgct gcgggtcact tgccagcata gcaatggctt tattcataaa atcatttttc
      421 gcccatgctt caagctgcag atatactaca ccggcgttga aataattccc tggaagtccc
      481 tctatagcca gacgcttcgc tggtttttcc tgcataaaag gcacatcttt aataacagct
      541 gcaaacttat ccccatcaaa attaatatca agcaatccag ctaatgagcc tttacagatg
      601 acatcagcat cgatatataa cagattttca atagtttcac tcagatactg gaacgcaact
      661 aaacggaaga atgttgcata agaccactgt ttcaggctgg ggaagatatc aataaaggca
      721 ctggagactt tataaattct tacttcaacg ttagcatttt tgctggtaaa agcattaaca
      781 cgttgtagat aatcttcatc aatgtagtcg gtaaagagat gaaacttaac cgcaaggtcc
      841 ggattatgca taacaactga ttgcatagat actgccgcac caaataaaaa gttacggtca
      901 ataccgtaag cgatatttaa cacacgatcg gtgttagcaa tttccccaga gacagcgact
      961 tcagtaatac tctcagctaa taaatccatg ttggttccgt tttgactgtg tggtttacgc
     1021 tttgcctttt aattttttaa taaatttgcg tagtttggtt ctgtagatta ctgaatagta
     1081 gccataatct tttatttcgt tggcaatacc caggtgacgt tccatggcca gacgatcacg
     1141 agctttacgc tctgccgtac agcttttacc ggataggtca attatcctga caccatcttt
     1201 actaacgata aagttacccc ggtgtgggtc gcccgatagc atatttaagg catgcaattt
     1261 ttccattgaa gccttaattt ctgctttaac atcttctgga atgatcggca tatcattgag
     1321 ctctacgccc tcgacatact cgataagcat gataaaaaca ctggcatagt tgaaaatttt
     1381 acgctcagcc agaaaataaa aatcattagg aaaagtaaga cctgcacttc tgacacgatc
     1441 ggtttcaacg atcaaattct gatagtagtc accttttaca aaagatttta aaaaacgttc
     1501 attccttttc gttttcgggg caaagacttt aaaaacatag cgacaatatt tcgtatcaat
     1561 caatgatact ttggtatcat taatattacg taatactttt actgttttaa aattataggc
     1621 cagtatttca tcaaagattt ctttatattt attatcatta tctttgtaat aaaatgagaa
     1681 gccgcgatag cgtatacttg taatcataag agtaatttat ttcttaagct tgtacttaat
     1741 taatgaagtt atgcctttaa tatactcacc atgggcaaac agatgcttat attgtttctg
     1801 catttctgca acagtccgcg cctctttaag aggatatttc ttccagggag attgttcacg
     1861 cgcgatatta aagtatgatg cagacggata accagcccag ctatgccatg gtttagttat
     1921 ccctgtatag tgaatcaata ctgttttatc ggtaatggtt tgctgatact ttcgatgact
     1981 acgatcatga agttcgtttt tcagggtata aatagtatca aaatccttcg cgaggtagat
     2041 attattcata ttaaatgcga tattcaacgc atcctgatct aaatatttaa gagagccata
     2101 tttagtttcc cctcgtaaaa gcgtgagtaa atacggtgtc aaatttgctt catgccattt
     2161 tttaatattg acatagataa cgcctgcatt gaaataacgt ccattcatct cgggaatgtt
     2221 gaggcgtttt gcacatgccg cctgagtact aacattgtca ggaatgaccg cagcaaactc
     2281 atctttaaat ataatttctg tgagaggctt cagacttcct ttacaaacga catcggcatc
     2341 cagatacagc agtgtggaaa tactttcact cagatattca aaggacaata ccctgaagta
     2401 tgtcgcgtac gaccaaaact gtgacgtcgg cagatcagca aagtattttg ggtca
//
LOCUS       DAZPJR010000309         2447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPJR010000309 DAZPJR010000000
VERSION     DAZPJR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.315"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(26..316)
                     /locus_tag="ACHVB7_004407"
     CDS             complement(26..316)
                     /locus_tag="ACHVB7_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR1916193.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(306..743)
                     /gene="ratA"
                     /locus_tag="ACHVB7_004408"
     CDS             complement(306..743)
                     /gene="ratA"
                     /locus_tag="ACHVB7_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR1916194.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            914..1396
                     /gene="smpB"
                     /locus_tag="ACHVB7_004409"
     CDS             914..1396
                     /gene="smpB"
                     /locus_tag="ACHVB7_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR1916195.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1611..1973
                     /gene="ssrA"
                     /locus_tag="ACHVB7_004410"
     tmRNA           1611..1973
                     /gene="ssrA"
                     /locus_tag="ACHVB7_004410"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2172..>2447)
                     /locus_tag="ACHVB7_004411"
     CDS             complement(2172..>2447)
                     /locus_tag="ACHVB7_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1916196.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          655 c          549 g          576 t
ORIGIN      
        1 tcaatgagca ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc
       61 gaagctcttt cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca
      121 cactatcgct tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga
      181 tatcggtacg caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct
      241 cctgcagcgt cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg
      301 caattttacc tagcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta
      361 gacgccagct ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca
      421 aagtcgagat gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca
      481 ccaatcaatt tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc
      541 aactggttgc gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca
      601 gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga
      661 ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt
      721 gcggtccggc taatctgagg cataactatt tccatcaaca aaaatccaac aaataatatc
      781 atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa
      841 gatagagcct tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg
      901 attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct
      961 taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct
     1021 gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt
     1081 ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc
     1141 ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga
     1201 actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct
     1261 gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca
     1321 cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa
     1381 aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc
     1441 tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc
     1501 attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat
     1561 tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt
     1621 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
     1681 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
     1741 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
     1801 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
     1861 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
     1921 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccacttttga
     1981 caggactgca accggacaga gactataaaa acagtcactt acggacactg accagacagt
     2041 aagcggacca gaaaaagaca aaaatataca cgcaaaatgc acgtgcattt caaaaaagcc
     2101 tctgattatg aagtcagagg cttttctagt tgtaaataag ggtaacaatt taacacgatg
     2161 cagcttacca attacgtaac atcaaacgct ctaattgttc ccaaatcctc cagaccactc
     2221 agctcctctt tcatttcacg ctgacggcga taaatctcat cgttgcgatc gacctgcgcc
     2281 tgcaccattg ctgccgccag ttcttccagt tccggcatcg acagtttcac ctgctgatta
     2341 tcggcatcac cccacgccat atgcgtttgt gctgtgacag attttgccag catgactacc
     2401 ggggacaggc ggcccagtga gtcggggcca gcattccaga tacgacc
//
LOCUS       DAZPJR010000310         2410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPJR010000310 DAZPJR010000000
VERSION     DAZPJR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.316"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..856)
                     /locus_tag="ACHVB7_004412"
     CDS             complement(<1..856)
                     /locus_tag="ACHVB7_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR1916197.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
     gene            complement(913..2091)
                     /gene="allB"
                     /locus_tag="ACHVB7_004413"
                     /pseudo
     CDS             complement(913..2091)
                     /gene="allB"
                     /locus_tag="ACHVB7_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(2010..>2410)
                     /locus_tag="ACHVB7_004414"
     CDS             complement(2010..>2410)
                     /locus_tag="ACHVB7_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR1916198.1"
                     /translation="GFHLTGTRQALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQ
                     WADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRW
                     MDPYFLNRVVATGKVMKPVLAQRQKVVSPP"
BASE COUNT          627 a          666 c          590 g          527 t
ORIGIN      
        1 atccggtcgc cacaaagctg cgacgataac gcgtattccc cgcgccctgc tgcggataaa
       61 aaacatccgt gccccgaatc gcaccgtagg tattgctgat atttaccaga cctgtaatca
      121 ccgccgtcag aataattccc ggcgacaaag caccgccact gccgagcggg aaccactgcc
      181 agtgcaactc accggagagc gaatgcgaag aaggaaagca aaagtaccac aacaaccagc
      241 cggttatggt gccgaccagc aggccataac gggcaaaacg ttgcggcaga aagataatca
      301 tcgccagtac caggcacatc accgccaccg agagcgcgaa cggtggtaac tgaattttaa
      361 aatatgggtc ggctatgcca aacggcagcc cgagcatacc tttgaaaaag atagtggtca
      421 gctgtgcgcc cagcatcaac ataaacaaga ccatcaccga cggcgtaaat aaccgtgcca
      481 ggcgatggcc taatccgcta aaaccaatca acatcgtcag cacgccggag agtgcaatcc
      541 ccaccgccag actggtagcg atatcgttga tcggtgtccc gcgcgatgct tctccaaggg
      601 tgatagtaag gatggttccc caccacaggc cacccggccc ttccataata gcgcgacgat
      661 gaccgcagaa cgcctgagcg aagcaggcca gtgcggtagc aagaaaagca tattgcgtga
      721 gcgtaagcag gctactttgc ggcaactgaa aagcagaaag tagcgtagga ggaaccacaa
      781 ccgtgttgca aaaaataaag aaaaaccact gaaatcctga taacaggctt tcgcggctga
      841 ctgcaaaatt gaacataaac acaccttaac cggagaatgc ccgccgcaag gacgggcatt
      901 gcaggggcca gattactgct gatgtttaag gataaattga cctttcggcg caacagggaa
      961 gccctgttca atgtcgtaaa tcacatcacc acgtaagatg gttttcgtga tacgcgcgcc
     1021 aatggtacgg ccaacatacg ggctgacttt gtggcgatat tccagatcgt cattggtaag
     1081 aacatagctg ctattcggct gaatgaagac gaagtcggca tcttttcctg gggcgatacg
     1141 gcctttttgc tgcagaccga aaatatctgc tgcgttagtc gccattaatt tgccgaacat
     1201 tggcagagac attccgcgtt tctgtaccgc ttcatcgaac atcacgtcca tacagttttg
     1261 cagaccggca ataccgcccc atgcttccat gatgttgccg gctttcattt ccggagggca
     1321 tggtgagtgg tcggaaacca ggcagtcgat ttcaccgtta aacagttttt cccacatgcc
     1381 tttctggttt tccagatcgc ggatcggcgg tgaacacttc gccagagtac cgatttcttc
     1441 gaactgatcg gtatccagta caaagtaatg cgggcaggat tcacaggtaa catcctgacc
     1501 ttcctgacgt gcacgagtca cttcttcaac actttccggg ctgctgatat ggcaaacgtg
     1561 cagacggcaa ccggcaactt tcgccagata cagcacgcgg cgaatcgctt ccacttcggt
     1621 aaataccgga cgcgaagcca catagtcatg ggcagttacg cgaccttcac gtttcgcttc
     1681 ttcgcccggt gcgtcacaga tcagcgcgtt ttcgcagtgc accaacaccg gctgccccag
     1741 ttcgcccagc ttctgcgcac ctttgaagaa ctgccagtcg tttacatcac ggaagtcgtt
     1801 gtcgataccg cgatcgccac aggtcgcaac gaagcatttg aagccgacaa cgcccacttc
     1861 atccagctca tgcagacggt cgatgttgta agacaccagg ccaccgagtt gtgccgcatc
     1921 gatagtcagc ttgcctttag cggcatcgaa cttcagttca attgaagcgc ggtcaaccgt
     1981 tgcaggcagc tggttgagcg gcatttcgat catggtggtg ataccacctt ttgccgctgc
     2041 gcgagtaccg gtttcataac cttcccagtg gctacgaccc ggttcagaaa gtacggatcc
     2101 atccagcgta aaccggcttc cgtggtggtt tggggtttat attcacaccc aacccaaccg
     2161 ttgtagtcag aattttcgat taccttaaag agataatcat aattaatttc tccggtcccc
     2221 ggttcgccgc gatgcggatt atcagcaatt tgcaggtgac caattttatc ggcccactga
     2281 gtcatggtgt tggttaattc accttccatc cgctgcatgt gataaatgtc atactgaatt
     2341 ttcaaattgc agcaaccaac atcatcaatc aatttcagcg cctgccgagt tccggtgaga
     2401 tggaaaccag
//
LOCUS       DAZPJR010000311         2402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPJR010000311 DAZPJR010000000
VERSION     DAZPJR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.317"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            75..164
                     /locus_tag="ACHVB7_004415"
                     /pseudo
     CDS             75..164
                     /locus_tag="ACHVB7_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="ACHVB7_004416"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="ACHVB7_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFR1916199.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..1962
                     /gene="hipA"
                     /locus_tag="ACHVB7_004417"
                     /pseudo
     CDS             641..1962
                     /gene="hipA"
                     /locus_tag="ACHVB7_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2146..2247
                     /locus_tag="ACHVB7_004418"
                     /pseudo
     CDS             2146..2247
                     /locus_tag="ACHVB7_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          666 a          505 c          618 g          613 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagccc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaataacatc
     1681 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     1741 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     1801 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     1861 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     1921 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     1981 tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta tgtttgtgcg
     2041 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     2101 tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac gaatatactg
     2161 ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc aaacgctgat
     2221 gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg tcatctgaaa
     2281 ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caattcacca atgtgaatct
     2341 cattattaat gaggagtggt ttgtatttgg agcgctatga aaaatcacat aatatctaaa
     2401 ct
//
LOCUS       DAZPJR010000312         2330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPJR010000312 DAZPJR010000000
VERSION     DAZPJR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.318"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            20..238
                     /locus_tag="ACHVB7_004419"
     CDS             20..238
                     /locus_tag="ACHVB7_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1916200.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            240..605
                     /locus_tag="ACHVB7_004420"
     CDS             240..605
                     /locus_tag="ACHVB7_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1916201.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            602..1267
                     /locus_tag="ACHVB7_004421"
     CDS             602..1267
                     /locus_tag="ACHVB7_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916202.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1267..1632
                     /locus_tag="ACHVB7_004422"
     CDS             1267..1632
                     /locus_tag="ACHVB7_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1916203.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2087..2242
                     /locus_tag="ACHVB7_004423"
     CDS             2087..2242
                     /locus_tag="ACHVB7_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1916204.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          627 a          496 c          628 g          579 t
ORIGIN      
        1 cccgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
       61 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct
      121 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
      181 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
      241 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
      301 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      361 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc
      421 acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg
      481 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      541 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      601 catgaccact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
      661 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
      721 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
      781 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
      841 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
      901 agttgaccag gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg
      961 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1021 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     1081 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     1141 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     1201 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     1261 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     1321 tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc
     1381 atgcgtaagg ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca
     1441 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     1501 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     1561 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     1621 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     1681 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     1741 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     1801 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat
     1861 agatggtgct ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg
     1921 tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt
     1981 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     2041 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     2101 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     2161 gaggaaagac ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt
     2221 agactacgaa tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg
     2281 atgtgtcagg catcctcaac gcacccgcac ttaacccgtt tcggcggttt
//
LOCUS       DAZPJR010000313         2330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPJR010000313 DAZPJR010000000
VERSION     DAZPJR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.319"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            173..799
                     /locus_tag="ACHVB7_004424"
     CDS             173..799
                     /locus_tag="ACHVB7_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR1916205.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            820..>2330
                     /locus_tag="ACHVB7_004425"
     CDS             820..>2330
                     /locus_tag="ACHVB7_004425"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR1916206.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINF"
BASE COUNT          622 a          498 c          613 g          597 t
ORIGIN      
        1 ttttctccta accataaagg attggtttat cggcgttaaa tcttttctca tacactatcg
       61 ggttagggaa aatatctggt gttggggaca gagtcagcag cgtattagtt gaatgcttaa
      121 tatcaggctg ctgaattgtc agcactgtgt ttgcgaagag gatgcgaaat aaatgaaccc
      181 ggttgatcgt ccactattag atattggctt aacgcgatta gaattcttgc gtatatcagg
      241 aaaaggcctt gctggtctaa caattgcccc tgcgttactg tcacttttgg gttgcaaaca
      301 ggaagatatt gatagcggca cagtagggtt gataaataca cccaaagggg tgctggtgac
      361 ccagcgagcg cgatgcacgg gttgccaccg ctgtgaaatc tcttgtacca acttcaacga
      421 tggctcagta gggacattct tctcccgtat caaaatccat cgcaattatt tctttggcga
      481 caacggggtt ggctctggcg gcggcctgta tggcgatctc aactataccg cggacacctg
      541 ccgtcaatgc aaagagccgc aatgcatgaa cgtctgcccg attggtgcga ttacctggca
      601 gcagaaagaa ggctgtatta ccgtcgatca taaacgctgt attggctgta gcgcctgtac
      661 tacggcatgc ccgtggatga tggccaccgt aaataccgaa agtaaaaaat cctcgaaatg
      721 tgtgttatgc ggtgaatgcg caaacgcctg cccaacaggg gcgttaaaaa ttatcgagtg
      781 gaaagatatt actgtgtgaa tcttgcaaag gaaaaaatca tggctaacgg ttggacaggt
      841 aatatattaa gagtcaatct cacgacagga aatattaccc tcgaagattc cagtaagttt
      901 aaaagttttg tcggtggcat gggcttcggc tacaaaatta tgtatgacga agtaccgcca
      961 ggcacgaaac ctttcgatga agcgaataaa ttagtctttg ctaccggccc attaactgga
     1021 tctggtgccc cctgtagttc tcgcgtaaat atcacctcac tttctacttt taccaaagga
     1081 aatttagtcg tcgatgccca tatgggtggc ttttttgcag cgcaaatgaa attcgctgga
     1141 tacgacgtca ttattatcga agggaaggcg aaatcaccgg tatggctgaa gattaaagat
     1201 gacaaagtta gcctggaaaa agccgatttc ttatggggaa aagggacgcg cgcaacgacg
     1261 gaagaaattt gtcgattgac cagtccggaa acctgtgtgg cggctattgg tcaggctggg
     1321 gaaaaccttg ttcctctctc tggcatgttg aatagccgta accacagcgg cggtgcggga
     1381 actggcgcaa taatgggttc gaaaaacctg aaagcgattg cggttgaagg gacgaaaggg
     1441 gtcaacattg ccgatcgtca ggagatgaag cgtctcaacg attacatgat gactgaactt
     1501 attggtgcga ataacaacca tgtcgtgcca agtacgccgc aatcgtgggc agagtattca
     1561 gatcccaagt cacgttggac agcacgtaaa gggctgtttt ggggcgcggc tgaaggtggt
     1621 ccgattgaaa cgggggaaat tccgccaggc aatcagaaca cggtcggctt tcgtacctat
     1681 aaatccgttt ttgacttagg tccggcggca gagaaataca cagtaaaaat gagtggctgc
     1741 cactcttgcc cgatccgttg tatgacccaa atgaatattc ctcgggtgaa agagtttggc
     1801 gtgcccagca caggtggtaa cacttgtgta gccaactttg tccataccac catcttcccg
     1861 aacggtccga aagattttga agataaagac gatggtcgtg tgattggtaa cctggtgggt
     1921 ctgaatctgt tcgatgacta cggcctatgg tgtaactacg ggcagttgca tcgcgacttt
     1981 acctattgtt acagcaaagg tgtgttcaag cgtgttctgc ctgctgaaga gtatgcggaa
     2041 attcgctggg atcaactgga agcgggtgac gttaacttca ttaaaattaa agatttttac
     2101 taccgtctgg cgcatcgtgt gggtgagctg agtcacctgg ctgatggttc atatgccatc
     2161 gcagaacgct ggaatttggg tgaagagtac tggggctacg cgaaaaataa actctggtcg
     2221 ccgtttggct atccggttca ccatgccaat gaagcgtcag cgcaggtcgg ttccattgtt
     2281 aactgtatgt tcaaccgtga ctgcatgacg catacccata tcaactttat
//
LOCUS       DAZPJR010000314         2296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPJR010000314 DAZPJR010000000
VERSION     DAZPJR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.320"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..431)
                     /locus_tag="ACHVB7_004426"
     CDS             complement(<1..431)
                     /locus_tag="ACHVB7_004426"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1916207.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
     gene            533..757
                     /locus_tag="ACHVB7_004427"
     CDS             533..757
                     /locus_tag="ACHVB7_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1916208.1"
                     /translation="MQQEPNGWKTVTTQFYIQEFSEYIAPLQDAVDLEIATEEERSLL
                     EAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            869..1132
                     /locus_tag="ACHVB7_004428"
                     /pseudo
     CDS             869..1132
                     /locus_tag="ACHVB7_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(1169..1726)
                     /locus_tag="ACHVB7_004429"
     CDS             complement(1169..1726)
                     /locus_tag="ACHVB7_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1916209.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1723..1998)
                     /locus_tag="ACHVB7_004430"
     CDS             complement(1723..1998)
                     /locus_tag="ACHVB7_004430"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR1916210.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            2183..2296
                     /locus_tag="ACHVB7_004431"
                     /pseudo
     CDS             2183..2296
                     /locus_tag="ACHVB7_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          651 a          471 c          543 g          631 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt ttagccccat catctgtgtt tgttttcatt
      421 gatttcacca tgttatagtt ttatttgtga attaaatcaa ttatggcgat gaattacaag
      481 gggttaaatg ctgccgcagc atagcgatat tgaaatagcc tagtatgctt cgatacagca
      541 ggagccgaat ggctggaaga ccgtcaccac acagttctac atccaggaat ttagtgagta
      601 tattgcgcca ctgcaggatg ctgtagatct ggaaatcgca acggaggaag aaagatcgtt
      661 gctggaggca tggaataaat atcgggtatt gttgaatcgt gttgatacat caactgcacc
      721 tgatattgag tggcctgcaa atcctgtcag ggagtaatca ttgggattat gccgcagaca
      781 cgtcgtatgc aggaacgtgc tgcggttagt ttgtgagctt tcgatagtgg ttgttatttt
      841 tgcccttatt tgttccggag gccatggttc aatggtccgt ctgccccctg tggtgatgtc
      901 agcaaaatca gccactgcgc gaaccacaat agcccgggaa gatgctgaag atcaccaggt
      961 aaagctgtca gcgcagaaac tggaagaact gctcgcatca atggttaagg atgaggttga
     1021 tcgcaatgat gggatttatt gacgtcagcg agagcagaag gaagaactga ataacctgaa
     1081 tgatttacgc tcaatcagag cgatggcgat tagcggcaat cacgctcgat aaaattaacc
     1141 tcggtgatca tccggggttt tgcattcatt aaaaccgcat caacctttcc accaattgtt
     1201 ctttacgggc aacgatccac ccatgttgct ccagataaaa tttaaaccgt tccagagtgc
     1261 ataccatcgc atcggcgggg actttttctg tgaactcgac ctgaccgtgt ttatcgaagt
     1321 ggatcagtaa tgcgcatcca ttatttgggg cgggggagtt ttgtgctgca ggaggacgtt
     1381 tgtggagttc ttcttccatt gcgttaaatt tttcaatgta agcttctttt agtctggctg
     1441 cttttttgcc agtaaatccc attacgagga acatgaagcc gtcttttgtg atcaggtagc
     1501 atcgtgattc acgttctgcg ccgttgccaa tttcgacagt ctgaacatcg gccgaaaagt
     1561 tggctgatgt aaattcggga gagcagtcga gtgtgtcaat cttagctaaa acgtgtttat
     1621 gttgtttatc gaagtagtcg gcaacagctt gagaggtggt aaccaggcga ccatctttga
     1681 aaaagacttt tggtgactcg ttagaaaaag ttatagctgt attcatatgt gatgactcca
     1741 attaattgga tgatcaccac ctctgacgcc aatcagggag gtggtgagac gtacagggtt
     1801 ggcgtaaccg gtcatcacaa aacccggcgc ggatttctcc gcccctgcac gccccaccat
     1861 aatgcgaatg tggcaatgct taacgcaaaa aaaaccgcaa acgcggctat gcgttgtgat
     1921 gttatccgag acgccaatcc cgacaaccga ttttgcggtt gtgtgaggaa tatagccccg
     1981 gagaacaaaa actgtcaacg ggcaaaaaaa tttccgttat caggaatgta taaaattaaa
     2041 gcgatcgagc aaaaaatcct ttctgcaaaa ctaaaaaaga tttcaataaa atcaggcagt
     2101 taaaaacacg gaaaacggta taaaaaagcg cctcaaaatc ctgttttttc tttcagatac
     2161 aatgtcttat tgcatttttt aaaacatgtc agtgtggtac atggatatcg ataccatcgc
     2221 caattataaa ctgggcggtg cacagcaaca tgatagcgta cgcctcgatc cgtgggtgtt
     2281 tatgttctca gcagga
//
LOCUS       DAZPJR010000315         2283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPJR010000315 DAZPJR010000000
VERSION     DAZPJR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.321"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHVB7_004432"
     CDS             complement(<1..304)
                     /locus_tag="ACHVB7_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1916211.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
     gene            complement(343..630)
                     /locus_tag="ACHVB7_004433"
     CDS             complement(343..630)
                     /locus_tag="ACHVB7_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916212.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(880..1035)
                     /gene="hokD"
                     /locus_tag="ACHVB7_004434"
     CDS             complement(880..1035)
                     /gene="hokD"
                     /locus_tag="ACHVB7_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1916213.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1263..1928)
                     /locus_tag="ACHVB7_004435"
     CDS             complement(1263..1928)
                     /locus_tag="ACHVB7_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR1916214.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2097..>2283)
                     /locus_tag="ACHVB7_004436"
     CDS             complement(2097..>2283)
                     /locus_tag="ACHVB7_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916215.1"
                     /translation="GAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQI
                     ERNAKARIRRATKSRTE"
BASE COUNT          568 a          595 c          508 g          612 t
ORIGIN      
        1 gctctgcggc ttcgctcaga gctggcgtat gttcccggcc tactgattca cacaggtaat
       61 caacgaaacc ggacaccagt tttcgttcgc gaggcgtgat cgccccaccg accggagtcc
      121 agtaatcgaa acccagttgc aggagtttga aaaaacgctt gtggaatgcg tagttacaca
      181 cacgcttaaa gtctgcgtgt atccactcac ctattttgat ttgatgcaga aaatcgcaac
      241 tctccggcgt cgccgggaga agtaatccgg aagaggtttg tttgaccagt tgtatatgtg
      301 ccatcggctt tctccggtgg cacggtgtta cacagcagga gttcaatcct gctcaagatt
      361 gtagatgagt ttattcttca gcaaaagcag aaaaccagcc ttaaaaccaa tctctttcaa
      421 aacccgtaat gatgtgacaa attcgtcctc acgcaaaata aaaccgtccg tcagaagtcc
      481 attacaaaaa taaaataaca cagcaccgct cttcctttgt tgagattgca aacatctaat
      541 gcggcaatgg ctgacaatcg ctccattctc aacgcgcaca gcatagaggc cattttcact
      601 aaaaatttca cgcaattctt cgattttcat cttcagaatc cttccagata aatagctctc
      661 ccctgttcgg ggtccatccc tcttctccct gcgcgctact taagtgagtc gattctatct
      721 gcgaaggtgc gcgaatcaaa ttcaccggaa ataaacaaca aaaaacccgc cgaaacgctg
      781 agagatcaca gcgatgggcg ggttaagtgc gggtgcgttg aggatgcctg acacatcaga
      841 ggtgggcggg gatgggatca gctccccgcc cggtcactct tacttcctgg attcgtagtc
      901 tacgaagaca gcgacctccg tctggccggt tcggattcgt acctcacaga ggtctttcct
      961 cgttaccagt gccgtcacta tgacggttaa acagatgacg atcagggcga ttaacatcgc
     1021 cttttgctgc ttcatagcct gcttctcctt gcctttcggc atgtaagagg ctaacctaca
     1081 tgtgcaaagc atgaaattgg cctcagatta atgttaagcg tcttgccgga cgcgtaatgt
     1141 taactggggc ttttctctat ctgccttttg gtgttcatgc ctgaagcaga tagcctcaag
     1201 cacccgcagt cattctactt acctcgccaa tatgaaatca atcagaaagg tgccccataa
     1261 aatcactcct tctcttcttt accgtagtgg agttgaccaa ttttgataag agggcgtccc
     1321 tgagatttgc ggtgtagatt ggtatcgcgc agagaataca cacagccaca atattcctgc
     1381 tgatagaatt tttcgcgctt gctgatttca atcatacggg acgagccgcc ctgcttgcgc
     1441 cagttataat cccagtacac catgcccgga taatgcgcga cggctcgccg cccacagtcg
     1501 ttaacctgct gcatattttt ccagcgtgaa atgcccagtg aactgctgat cacactgaaa
     1561 ccattttcag cggcgtacag cgctgtccgc tcaaaacgca tgtcaaaaca catggtgcaa
     1621 cggatccctc gttcgggctc ccattccatt cctttggctc gttcaaacca gttgtctgtg
     1681 tcgtaatcag catcaataaa cggcacgccg tgttgttcag caaagcgaat attctcatcc
     1741 ttacgaatta aatactcttt ctgaggatga atgttcgggt tgtagaaaaa gatggtgtag
     1801 tcgattcccg aggcctgaag cgcctccatc acctcaccgg aacatggagc acagcaagag
     1861 tgcagtagta gtttgtttgc cccgtttggg agctccaatt tgggccgttt gaaatcagca
     1921 actgtcataa atatgtttat tggggtcata aaaatatcaa agagtgtagc attagaacgg
     1981 ggctatcgga aacagatgtg tgactgctcc ccgccctttc gggtggtctc ctgatgattt
     2041 gagggtgcag aaatccctcc ggttaaggat taaattttta acagtgctaa atttaattat
     2101 tcagttctgg attttgtcgc cctgcgtatc cgcgctttcg cgttacgctc aatctgtatc
     2161 agcttttcta tatttctccg cctttcctgt tcctcctggc gcaatagcct tacatcatct
     2221 gccagtctgg tttctctttt tgccacagag agcatccagt caaacggctc cacaaccgca
     2281 ccg
//
LOCUS       DAZPJR010000316         2275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPJR010000316 DAZPJR010000000
VERSION     DAZPJR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.322"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            145..669
                     /gene="aroL"
                     /locus_tag="ACHVB7_004437"
     CDS             145..669
                     /gene="aroL"
                     /locus_tag="ACHVB7_004437"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR1916216.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            719..910
                     /gene="yaiA"
                     /locus_tag="ACHVB7_004438"
     CDS             719..910
                     /gene="yaiA"
                     /locus_tag="ACHVB7_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR1916217.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            1168..1845
                     /locus_tag="ACHVB7_004439"
     CDS             1168..1845
                     /locus_tag="ACHVB7_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR1916218.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            1917..2201
                     /gene="ppnP"
                     /locus_tag="ACHVB7_004440"
     CDS             1917..2201
                     /gene="ppnP"
                     /locus_tag="ACHVB7_004440"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR1916219.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          603 a          506 c          570 g          596 t
ORIGIN      
        1 ttggtatagt aagggatgta ttgagatttt cactttaagt ggaatttttt ctttacaatc
       61 gaaattgtac tagtttgatg gtatgatcgc tattctcatg acaccggctt tcgccgcatt
      121 gcgacctatt ggggaaaacc cacgatgaca caacctcttt ttctgatcgg gcctcggggc
      181 tgtggtaaaa caacggtcgg aatggccctt gccgattcgc ttaaccgtcg gtttgtcgat
      241 accgatcagt ggttgcaatc acagctcaat atgacggtcg cggagatcgt cgaaagggaa
      301 gagtgggcgg gatttcgcgc cagagaaacg gcggcgctgg aagcggtaac tgcgccatcc
      361 accgttatcg ctacaggcgg cggcattatt ctgacggaat ttaatcgtca cttcatgcaa
      421 aataacggga tcgtggttta tttgtgtgcg ccagtatcag tcctggttaa ccgactgcaa
      481 gctgcaccgg aagaagattt acggccaacc ttaacgggaa aaccgctgag cgaagaagtt
      541 caggaagtgc tggaagaacg cgatgcgcta tatcgcgaag ttgcgcatat tatcatcgac
      601 gcaacaaacg aacccagcca ggtgatttct gaaattcgta gcgccctggc acagacgatc
      661 aattgttgat tttagagcgc ctatacttaa cgttcatccc gtgaaataag gaagaacgat
      721 gccaacgaaa ccgccttatc ctcgtgaagc atatatagtg acgattgaaa aaggaaagcc
      781 aggacagacg gtaacctggt accaactcag agccgatcat cctaaaccag actcgttgat
      841 cagtgaacat ccgacggctc aggaagcgat ggatgcgaaa aaacgctatg aggaccctga
      901 caaagagtga ccgcatcaga ctgctcggaa gggattctga gtgccactac aagggatctg
      961 cgtcacattt ttcataattc atgtttttct aataattaga atattaaaca ataacaatcc
     1021 attactggaa tcatttggaa tctttacatt atgccgtgca cgtctgctgc tacgcttttt
     1081 gtcatttgta gcacaagtaa gtgtcagcag tggtgctcca cacttgcccg gtaattaacg
     1141 acgaaagaaa agtaaggtgg atgaacaatg agtgcgtcgt tggcgatcct gaccatcggc
     1201 attgtaccta tgcaggaggt tttgccgctc ctgacggaat acattgacga agataatatt
     1261 tcccatcata gcctgctggg gaagttaagt cgtgaagaag tgatggcgga gtacgcgcca
     1321 gaagcaggcg aagacaccat tctcacatta ttaaatgaca accagctggc ccatgtttcg
     1381 cgtcgtaaag tggagcgaga cctgcaaggt gtggttgaag tgctcgataa tcagggttat
     1441 gacgtcattt tattaatgag tacagcaaac attagtagta tgactgcgcg taatacgatc
     1501 tttcttgagc cgtcgcgaat attgcctcca ctggtttcct ctattgttga agatcatcag
     1561 gtgggggtta tcgttccggt tgaggagatg ctgaccgttc aggcgcaaaa atggcaaatt
     1621 ttgcagaaat cgccggtatt ttcattgggt aaccccattc atgattcaga acaaaaaatc
     1681 attgatgccg ggaaagaatt actggcaaaa ggggctgatg tcatcatgct ggattgttta
     1741 ggatttcacc aacgtcatcg cgatttactg caaaaacagc tcgatgttcc tgtcttgctg
     1801 tctaacgtat tgattgcacg gctggctgcg gaattactgg tgtaattttg cgtgacagcc
     1861 agcgcctctg gcccctatag tgaagtagat gttcaactac cagacagggc cagtttatgc
     1921 ttcaaagtaa tgagtacttt tccggcaaag tgaaatcaat cggcttttcc agcagcagca
     1981 ctggtcgcgc cagcgtgggt gttatggttg aaggcgaata caccttcagc accgctgagc
     2041 cggaagagat gacggtaatc agtggcgcgc tgaatgtgtt actgcctgac gcgaccgact
     2101 ggcaggtgta tgaagccggt tcggtgttta atgttcccgg tcacagtgag tttcatctgc
     2161 aagttgccga acccacctct tatctgtgcc gctatctgta attcctcgcc ttccccttga
     2221 acgggagggc atttttctga aatatcctct ctttaaccca taataatatt tcctt
//
LOCUS       DAZPJR010000317         2267 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPJR010000317 DAZPJR010000000
VERSION     DAZPJR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.323"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(129..1133)
                     /gene="argF"
                     /locus_tag="ACHVB7_004441"
     CDS             complement(129..1133)
                     /gene="argF"
                     /locus_tag="ACHVB7_004441"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR1916220.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1295..1711
                     /gene="rraB"
                     /locus_tag="ACHVB7_004442"
     CDS             1295..1711
                     /gene="rraB"
                     /locus_tag="ACHVB7_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR1916221.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1757..2260)
                     /locus_tag="ACHVB7_004443"
     CDS             complement(1757..2260)
                     /locus_tag="ACHVB7_004443"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1916222.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          577 a          559 c          551 g          580 t
ORIGIN      
        1 ttaaatcctc cggaagattt acgtatagca ataaaaaaat tggggtggaa taattgaact
       61 atcactgcat aaaataatgt gacgccggga tgtttttgta tttcccggca tctttatagc
      121 gatatcaatt atttactgag cgtcgcgacc atcaccgctt tgatggtatg catgcggttt
      181 tccgcctgat caaatacaat gctggcggca gattcgaaga cctcatcagt cacttccatt
      241 ccgccatgta ggccaaactc ttccgccatt tttttgccaa gcgtcgtttg gtcgtcatga
      301 aacgcgggca ggcagtggag gaatttgacc tccgggttac cggtcaactg catcatcttg
      361 ctgttcactt gataatcacg cagcaatgca atgcgttcag cccatttctc ttttgcttca
      421 cccatcgaca cccatacatc ggtatagata aagtcagcac cttcaactcc tttagctaca
      481 tcttccgtca gcgtaatatt cccgccattt tgttgtgcca gggcgcggca ttccgtaacc
      541 agcgcagctt ccggccagca tgcctgtggc gcgaccagac gcaaatccag accggtaagc
      601 gctgcagctt cgagcatcga attgcccatg ttgttacgtg cgtcacctgc atagactagc
      661 gtcatttcgt tgaacgtttt gccgggcaaa tgctcctgca tggtgaggag atccgccagc
      721 agctgcgtgg ggtgaaactc attggtcagg ccgttccata ccggcacgcc agcgtattcc
      781 gccagtgttt cgacaatctc ctgaccatag ccgcgatact gaataccgtc atacatgcga
      841 ccaagcacgc gggcagtgtc tttaatcgac tctttatgac caatttggct gccgcttggg
      901 ccgagataag taacacgagc cccctggtca tatgcggcaa cttcgaaaga gcatcgggta
      961 cgggtcgagt ctttttcgaa gatgagcgcg atgtttttac cagtgagttt ggcttcttct
     1021 ttaccgcttt tcttatcggc tttcagcttc gcggctaact gcagcaggct gttgagttca
     1081 gctggcgtga aatcgagtaa tttcaggaaa tgcttatgat aaaacccgga cataggtccc
     1141 tcctgtggct aacgccttaa tgaattaaaa ttcaatttat atggatgatt attcatttgc
     1201 aagtctaaag cataaatctt tatcacaaag gtggaggcaa tgtcagtggt gtgtgacaat
     1261 aagagtatcg gcaggacatt aagaggaatg agccatggca aacccggaac aactggaaga
     1321 acagcgtgaa gaaacacgtt tgattattga agaattactg gaagatggca gcgatccgga
     1381 cgcgttgtac accatcgaac accatctttc cgcagacgat ctggaaactc tggaaaaagc
     1441 ggcagttgaa gcgtttaaac tcggttacga agtgaccgat ccagaagagc tggaagttga
     1501 agatggtgat atcgtgattt gctgcgacat cctcagcgag tgcgcgttga atgccgatct
     1561 gatcgatgcc caggttgaac aactgatgac gctggcggag aaatttgacg ttgagtacga
     1621 cggttggggc acttactttg aagatcctaa cggcgaagat ggcgacgatg aagattttgt
     1681 cgatgaagac gatgacgggg ttcgccacta attaattgat taaaatgccc gatagcacat
     1741 actagcgggc atttttttag agttcgcgta gcatccgcac ttcacaatcg acatggcccg
     1801 tgcagccaag cgcatagtcg atatgctcaa agcccaaatg ctcataaagc gcaatggctt
     1861 cctttaaaaa agcggtcgtt tccagatagc agcgtttgaa gcccatctct cgtgcctgct
     1921 ccatcgccat taaggccagt tttttcgcta gccctttacc gcgaatagcc ggaagaaaat
     1981 acatcttttg cagttcgcaa atgtccgact cactcccggt taatggcgca atcccaccgc
     2041 cgccgaccac ttcaccctcg tactcaacca cccaatatgc atggccaggc tgactataca
     2101 cttgatacag ctcgtcaaga ttcggatcgg cgacggtgta gcctttatca gcggtaagac
     2161 cgtattcggc ggatacctga cgaatgacgc gggcgatagc aggattatcc agcagcgtca
     2221 ggcgacgcat tacaggtgat tgcggcgcaa tgttattcat agtttaa
//
LOCUS       DAZPJR010000318         2225 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPJR010000318 DAZPJR010000000
VERSION     DAZPJR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.324"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..507
                     /locus_tag="ACHVB7_004444"
     CDS             <1..507
                     /locus_tag="ACHVB7_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1916223.1"
                     /translation="KIHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNF
                     NNKILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDT
                     IDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTL
                     SYREVNRD"
     gene            500..982
                     /gene="yqeJ"
                     /locus_tag="ACHVB7_004445"
     CDS             500..982
                     /gene="yqeJ"
                     /locus_tag="ACHVB7_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR1916224.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1016..1096)
                     /locus_tag="ACHVB7_004446"
     CDS             complement(1016..1096)
                     /locus_tag="ACHVB7_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916225.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1132..1556)
                     /gene="yqeK"
                     /locus_tag="ACHVB7_004447"
                     /pseudo
     CDS             complement(1132..1556)
                     /gene="yqeK"
                     /locus_tag="ACHVB7_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            1986..>2225
                     /locus_tag="ACHVB7_004448"
     CDS             1986..>2225
                     /locus_tag="ACHVB7_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916226.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMS"
BASE COUNT          820 a          364 c          370 g          671 t
ORIGIN      
        1 aagatccata atgacattaa catacaaaat catgtaataa actcgtcaac agacgcacat
       61 acacacaatg ccccacctgc cataaaagtt aatgcgggat acaaagagag cattggtggc
      121 gcaaagaatt tcaataacaa aatcctcaaa catataaaaa gtcatctaat tatgttgagc
      181 gcatttgtca taggcgcata ttctgcatat tggctatgga ataataatca accgaagcca
      241 tttttcaagg attacaagac tgttgcggaa attaatggct gccattttaa tgtaacagaa
      301 gatacaattg atgggttgaa agagttcgat aaatataaaa cacggatact ggattcgggg
      361 attaactgta aaaaatatcc gtggttatac ttccctcttg caaaatcctc accggggatg
      421 attgttatgg catgcaataa aaactataac caacatgaag tggcagactg tttaaccctg
      481 tcttaccgtg aggttaatcg tgattgatta taaaaaaaac ttattattta ttttagtttt
      541 tatcagtggg tttattctct tcactgtcta ttcttacact gcagaaaaaa tgatatataa
      601 cgaaacatgc actgcaaatt gggtaatttt taatgaccag ggacgagcaa atcttaccat
      661 cgactttatg tataacaaaa aaaataaaac aggaactgtt gcgttgagtg gaacctggca
      721 acaaggtaac agagaaagta aatcaatacg gcgaaatatt gaatacacat ggattgaaaa
      781 ctatgacaca gcccatttaa catcaaagaa agttaataag tttgagatta tggatcaagt
      841 tgatgatgat agacttgcac aacttattcc tgatttttat gtctttccag aaaaaagtgt
      901 aagctataat atactaaagc aaggtaagca tgcttttatt ttgagcattg gtaacagagc
      961 aataatgcat tgtgcaaggt aaactaaaat agataacaaa aaagatggaa cctcgttaat
     1021 cataaaacaa taaaattaaa tattctcgca gtatatggca gtctaaagca tcaaagtttt
     1081 gatcaacatc tttcatttta gacatctcct tgcaatgtaa aatatcatgt atcaacaaca
     1141 tctggtgcaa atccatgagt cgaactcgac attcatctta gctcgattat tattccttcg
     1201 tacagtcgat gtaaacaata cagaaagagg attattaaga accagtttac tcaggaatca
     1261 atagaggaaa ggactaacgt ttctttaaaa gaattgattt catcatctgt taaactaaac
     1321 tcatcattga cagatcgtga gatataactg tttttaactt tactctttac gttgacttta
     1381 ttgacagaat taacattcac atatcttgaa tttaatgtcc atgatgttgt ttgcgtgaga
     1441 acattctcag cattaaggaa tttttttaac ggaatccttg gtttcttttt cgagtccgaa
     1501 tttacaatat catgcatata gaccatttca tgaatctgcg aaaactcaat gtccattgta
     1561 tacctcacat ttttaccgtg actcgatgtt actgttcaat aatcaccttc catcaatact
     1621 aaaattaata cccctaatgt gccgataaca aatatagtca ttctacgtaa cgtctccata
     1681 aggtgataac cttgatcaaa tgaatttcga atataataaa ggaaatcttt tgagatgttt
     1741 atcacgaata aagctattta tgttaccaat atcatttcga gtgcgcacac tatctatctg
     1801 ttttttcgag atataaaaaa caacgtgtaa gttatgtaaa ttgccgatgc tactgagtaa
     1861 cattatggta aaaaaagtaa aaatgagcga tttttgatag aataaagcat gagaaaaggt
     1921 gatatttgac attatcagaa gctgcgaatt cggattttgc tctaatcaaa tgagagaaat
     1981 gtaaaatgaa gccacgaaat attaataata gcctaccact gcaaccatta gttcctgatc
     2041 aggagaacaa aaataagaaa aatgaagaga aatccgttaa tccagttaaa atcacaatgg
     2101 ggtctggttt aaattatatt gaacaagaat ctcttggagg aaaatatcta acacatgatt
     2161 tgtcaataaa gatagcggat atttctgaag agataattca gcaagcaata ttatctgcta
     2221 tgagc
//
LOCUS       DAZPJR010000319         2169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPJR010000319 DAZPJR010000000
VERSION     DAZPJR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.325"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..230)
                     /locus_tag="ACHVB7_004449"
     CDS             complement(<1..230)
                     /locus_tag="ACHVB7_004449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916227.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKTQV"
     gene            167..1666
                     /gene="pitB"
                     /locus_tag="ACHVB7_004450"
     CDS             167..1666
                     /gene="pitB"
                     /locus_tag="ACHVB7_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR1916228.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1715..>2169)
                     /locus_tag="ACHVB7_004451"
     CDS             complement(1715..>2169)
                     /locus_tag="ACHVB7_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HFR1916229.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
BASE COUNT          507 a          526 c          568 g          568 t
ORIGIN      
        1 acctgtgttt tgattatttc taaaggtttt gaatgaatgc ttattgtctg atacacaaga
       61 aataacactc tttttatcgt taaaaaatga tatttcactt tgcccatgcc gttaaagtca
      121 ctgataatgc gtccgttcgt aaattcaaaa tggcgtaatc taatatatgc taaatttatt
      181 tgttggcctt gatatataca cagggctttt gttattgctt gctctggcat ttgtgttgtt
      241 ctacgaagca atcaatggtt ttcatgacac ggcgaatgcg gtggcaaccg ttatttatac
      301 tcgtgccatg caaccacaac ttgctgtggt gatggcggca ttttttaact tttttggcgt
      361 gttattgggc ggacttagcg ttgcctatgc cattgtccat atgttgccaa ccgatttgtt
      421 gctgaatatg gggtcaaccc acggcctggc gatggtcttt tccatgctgc tggcggcgat
      481 tatctggaac ctgggaacgt ggttcttcgg tttaccggcc tccagttcgc acaccttgat
      541 tggtgcgatt atcggcatcg gtttaaccaa cgcgctgtta accggctcat cggtgatgga
      601 tgcgttaaac ctgcgtgaag tgaccaaaat tttctcctcg ctgattgttt cccctatcgt
      661 cggcctggtc attgcgggag gcctgatatt cctgctgcga cgctactgga gcgggacgaa
      721 aaagcgtgac cgtattcacc gcattccgga agatcgcaaa aagaaaaaag gcaaacgtaa
      781 accgccattc tggacgcgta ttgcgctgat tgtttccgct gcgggcgtgg cgttttcgca
      841 cggcgcgaac gacggacaaa aagggatcgg cctggtgatg ctggtactgg tggggattgc
      901 ccctgctggc ttcgtcgtca atatgaacgc gtccggctat gaaattaccc gtacccgcga
      961 tgccgtcacc aacttcgaac actacctgca acagcatcct gaactgccgc agaagttgat
     1021 tgcgatggaa cctccattgc ctgcagcatc gactgatggc acgcaagtaa cagagtttca
     1081 ctgtcatccg gcaaatacct ttgatgcgat tgcgcgcgtt aaaacgatgc tgccaggcaa
     1141 tatggaaagt tacgagccgt taagcgtgag tcagcgcagc cagctgcgcc gcattatgct
     1201 gtgcatctct gatacctccg cgaagctggc gaaactgcca ggcgtcagta aagaagacca
     1261 gaacctgctg aaaaaactgc gcagcgatat gttaagcacc attgagtacg ctccggtgtg
     1321 gatcatcatg gcggtagcac tggcgctcgg cattggcacc atgattggct ggcgtcgtgt
     1381 agcgatgacc atcggtgaga agattggtaa gcgcggcatg acgtatgcgc aaggcatggc
     1441 ggcacaaatg acggcggcag tgtctatcgg ccttgccagt tatattggga tgcctgtctc
     1501 cacaacacac gtcctctcgt ctgcggtagc agggacgatg gtggtggacg gcggtgggtt
     1561 acagcgtaaa acggtaacca gcatcctgat ggcgtgggtg tttactttac cggcggcaat
     1621 ttttctttct ggtgggctgt actggatagc attgcagttg atttaacctt cctgaaaatg
     1681 cccggtcctg gcgatcgggc atttccattt ttgactagtg ataaccacgc gcggtcataa
     1741 aatccgtaat cgctttttct gcatcaacca acacctgttc cagtggctga ttggcatcga
     1801 tatcaaccag ttgtgcacca ccaaaggtta actgtggcgt tatggcaatc ttcctcgcca
     1861 gcgattcccg tttatggtcg ggtttacgtg cacaggcaac ttcaaggtca acattgagtt
     1921 tgatgaccag atcaggctta tggctcgcca tccagtgaaa cgctttacgt tcctggcttg
     1981 ccagccatga gacaaaacga ccaccttcaa cgttaggtgg gaacaccgta ccatcgtaag
     2041 cgccaggaat ttggtcctga ggataacggt cggttagaac aattaacccg cgacgacgac
     2101 aggcaagcat atgacgaaag cgcagtaagc gacgggcgac aaacgctgta attaccagcg
     2161 ccggaactg
//
LOCUS       DAZPJR010000320         2114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPJR010000320 DAZPJR010000000
VERSION     DAZPJR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.326"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..410
                     /locus_tag="ACHVB7_004452"
     CDS             <1..410
                     /locus_tag="ACHVB7_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR1916230.1"
                     /translation="RPVQILYTYSDAGNSVFLVVDHLPWTDSDKINWYLKHQNEIKNQ
                     HPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMVINEY
                     PYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(464..1414)
                     /gene="corA"
                     /locus_tag="ACHVB7_004453"
     CDS             complement(464..1414)
                     /gene="corA"
                     /locus_tag="ACHVB7_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR1916231.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1566..1622)
                     /gene="ysgD"
                     /locus_tag="ACHVB7_004454"
     CDS             complement(1566..1622)
                     /gene="ysgD"
                     /locus_tag="ACHVB7_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR1916232.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1784..>2114
                     /locus_tag="ACHVB7_004455"
     CDS             1784..>2114
                     /locus_tag="ACHVB7_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916233.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          588 a          506 c          498 g          522 t
ORIGIN      
        1 tgcgtcctgt acagattttg tatacatact ctgatgctgg caattcagtc tttctggtcg
       61 ttgatcatct gccatggact gacagcgata aaataaattg gtatctgaag catcagaatg
      121 aaattaaaaa tcaacatccc ctaccagaag gtagttggca tacctggtat gtcattgata
      181 tcggtaatgg cttcactgac tataaaaaat atatcgaagg gccgtatgaa gatctctact
      241 gtttcccgac aattaagagc aatgataatt gcatcgtgaa aaattactta atggttatta
      301 atgaataccc ttatcgtaac actcatattg gtattaacga ttttactgag tatcaattaa
      361 cacaagaaaa taaaatagag cgagttttca atccgcatga ctttaaataa gacaattttt
      421 agaaataagc cggataaaaa aatagcttta tccggcactc atattacagc cagttcttgc
      481 gcttaaagta cagatacggt gccaggcccg cgaggatcat aaagataatc gcgcctgggt
      541 agccgaaact ccacttcagt tctggcataa actcaaagtt catcccatag ctggaagcaa
      601 cgagcgtcgg cggcaggaat accacggata ccaccgagaa gattttgatg atgcggttct
      661 gctcgatgtt gataaaaccc atcgccgcct gcatcaggaa gttcaccttc tggaacaggg
      721 attcgttatg cggcagcagg gattcgatat ctcgcaggat ttcacgcgcc tgctccagtt
      781 gcccacccgg taaacgcgct ttacgcacca ggaagttgag cgcgcgctgg gtatccatca
      841 gacacaggcg aactttccag ccgatatctt ccagttccgc cagagtggag agcgcctcgt
      901 cgtactcatc gccctgatgc ccttccataa tcacccggct caactgctcc aggtcgctat
      961 agatattttc aatttcatct gccaactgtt cgattttggt ttcgaacaga tccagcagca
     1021 gctcgtaggc gttaccgtct accatcgact ggctacgggc acgcatacga tacagacgaa
     1081 aagcgggcag ttcacgctca cgcagagtaa acagacgacc atcacggatg gtaaatgcca
     1141 cagtggagtt accggcgtga tcttccgcat cttcaaagaa gaagaaggag tgaatatgca
     1201 ggccgtcgtc gtcttcaaag aaacgtgccg atgcttcgat gtcttccagt tccgggcggg
     1261 ttgccaggct ctggccaagt tcagattgta cgcgcagtcg ctcgtcgtcg tccggttcga
     1321 caagatcaat ccatactgca tttacaaggg gttgtgactc ttcgacttcc agccgggtca
     1381 gtcggttatt ttccagttga aatgcgctca gcatgaccgg gactcccaat gcttaaaata
     1441 tcggacagtt cggtgggcac acagaaacaa attgggtttc agaccattaa acagcctgac
     1501 tcagcgcgac gggaaaaatt gaggtcgctg acaaccgcta aggctatcag caaaaaggga
     1561 tagccttagg agttgatcct ggatgacagg atagtgagcc agtatctact gggtgtgtcc
     1621 aaggcgaatg tcctcttaga gtgatcgtgc gcgcatgtta cgccagcaaa attttgccgt
     1681 caacacgcaa cagaacacca cagaacaata gttgaacttc gcgctttttg gtactcgtca
     1741 aggtcgctaa caggataaaa aaatccaaca tccaggacct ataatggcgc atcggctact
     1801 cattggtaaa ggaatgatca ccttgaatct caaacggatt tttctcgccc tcaccttact
     1861 tcccttattt gctgttgccg ctgatgattg cgcactctca gatccgatgc tgaccgtaca
     1921 ggcgtatacc gttaatcctc aaacagagcg ggtgaaaatg tactggcaaa aagccaatgg
     1981 cgaagcgtgg ggaactttac atgctctgct ggtggatatg aatagtcagg gtcaggtgca
     2041 gatggcgatg aacggcggca tctatgatga aagctatgcg ccgctcggtt tgtacatcga
     2101 aaacggtcag caga
//
LOCUS       DAZPJR010000321         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPJR010000321 DAZPJR010000000
VERSION     DAZPJR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.327"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHVB7_004456"
     CDS             complement(60..1025)
                     /locus_tag="ACHVB7_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR1916234.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHVB7_004457"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHVB7_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916235.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          437 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga gcccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZPJR010000322         2074 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPJR010000322 DAZPJR010000000
VERSION     DAZPJR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.328"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            3..699
                     /locus_tag="ACHVB7_004458"
                     /pseudo
     CDS             3..699
                     /locus_tag="ACHVB7_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1452
                     /locus_tag="ACHVB7_004459"
     CDS             706..1452
                     /locus_tag="ACHVB7_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1916236.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1467..1889
                     /locus_tag="ACHVB7_004460"
     CDS             1467..1889
                     /locus_tag="ACHVB7_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1916237.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1890..>2074
                     /locus_tag="ACHVB7_004461"
     CDS             1890..>2074
                     /locus_tag="ACHVB7_004461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916238.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT          568 a          456 c          604 g          446 t
ORIGIN      
        1 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
       61 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
      121 cgttaattca ggttggatgg ctggtcgaaa atgacggagg gctttctcta cctaactttg
      181 aacgtcataa cggaaaaagc tctaaaaaaa acgggcggtt acaaacgagc gagtaacaaa
      241 aatacgcgaa ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc
      301 gttaccagag gaagaggaag aggaagagga agaggaagag gaagaggaag atctaaatac
      361 tgatctcccc ctcgccaaaa acgagcgtct aaaaaattcg agccggaggc tattgagctg
      421 cccgattggt tgccggaaac actctggcat gagtgggtcc ggttcagaca ggcattgcga
      481 aaaccgattc gaacggagca gggcgctaac ggggcgatac gggaactgga aaaattccgt
      541 cagcagggtt ttacacctga gcaggtgatt cgacacagca tcgccaatga ataccagggc
      601 ctgttcgcgc cgaaaggtgt tcggcctgag acgttgctcc gacaggttaa caccgtctcg
      661 ttgccggaca gtgcgatccc gccaggcttc agggggtaac ggaccatgaa aaatattgcg
      721 acaggcggcg ttctggaacg catccgccga ctgaccccgc cacatgtaac cgccccattc
      781 agaacggtag cggagtggcg cgagtggcaa cttgctgaag gccagaaacg tagcgaggag
      841 atcaaccgcc tgaatcgcca gttgcgggtg gaaaaaattc tgaatcgctc aggcatccag
      901 ccgttgcacc gtaaatgctc gtttgcgaat taccaggtgc agaacgacgg ccagcgatac
      961 gcgttaagcc aggcgaaatc catcgccgat gaactgatga ccgggtgtac aaattttgcg
     1021 ttcagcggaa aacctggtac cgggaagaat cacttagcgg cagctatcgg gaatcgcctg
     1081 ctgaaagacg gtcagacagt gattgtggtt accgtggctg atgttatgag cgccctgcac
     1141 gccagctatg acgacgggca gtcaggcgaa aaatttttgc gggagctgtg cgaagtggat
     1201 ctgctggttc ttgatgaaat tggcattcag cgcgagacga aaaacgagca ggtggtgctg
     1261 caccagattg ttgatcgccg gacagcgtcg atgcgcagcg tggggatgct gacaaacctg
     1321 aactatgagg tgatgaaaac attgctcggc gagcgggtga tggatcgcat ggtcatgaac
     1381 ggcgggcgct gggtgaattt taactgggag agctggcgtc cgaatgttag ccattcgagg
     1441 gttgttaagt agtttcagga ggatttatgg cgaaaccttt tactcccgaa cagcgggaag
     1501 aactgaagac gcgaattgtg gaactcgtgc atcaggacgg tcgggtcacg attcggcagt
     1561 tgtcagatga aacaggtatc agtcgtgcgt ctgtcggtcg cttatgcata gaactggtcg
     1621 caagtggtga tgtatataat tctggctacg gcttattccc gtctgaacag gctcgcaagg
     1681 actggcaaag cgcccgcaaa aaactctcga gagtaaaggt gaggaaaccg gttgttgttg
     1741 atccggacct tatctggtca ttacctgacg gagaaatacg ccgctacgac aggcgcctga
     1801 atataatctg tcgcgagtgc cggaagagcg aagctatgca gcgtgtactg gctttctatc
     1861 agggtaattt tcaggaggcg atactgtgaa tgaaattagc tatcaggctt caattaccgc
     1921 tggcattcgc atcaaaggag aggagcatgg aaataaaacc agaagatgag ttaagcaata
     1981 tcgttttatt tccggtaaaa gaggatgacc ctcgtaatca ggttaatttt ctttatgagc
     2041 catcggaaag accatattgc catcacgcct ctgt
//
LOCUS       DAZPJR010000323         2010 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPJR010000323 DAZPJR010000000
VERSION     DAZPJR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.329"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(177..1037)
                     /locus_tag="ACHVB7_004462"
     CDS             complement(177..1037)
                     /locus_tag="ACHVB7_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR1916239.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1220..1651)
                     /locus_tag="ACHVB7_004463"
                     /pseudo
     CDS             complement(1220..1651)
                     /locus_tag="ACHVB7_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1717..>2010
                     /locus_tag="ACHVB7_004464"
     CDS             1717..>2010
                     /locus_tag="ACHVB7_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR1916240.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR"
BASE COUNT          500 a          517 c          465 g          528 t
ORIGIN      
        1 gccggacatc agcgctatct ctgctctcag gggtctgacg ctcagtggaa cgaaaactca
       61 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      481 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg cagcagtgtt atcactcatg
      661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      781 tgcccggcgt caacacggga taataccgca ccacatagca gaactttaaa agtgctcatc
      841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacga
     1021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta ccagggttat
     1081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1141 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1201 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatttta taaaccgtgg
     1261 agcgggcaat actgagctga tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg
     1321 tcagcacgac gttcctgtcc acggtacgcc tgcggccaaa tttgattcct ttcagctttg
     1381 cttcctgtcg gccctcattc gtgcgttcta ggatcctccg gcgttcagcc tgtgccacag
     1441 ccgacaggat ggtgaccacc atttgcccca tatcaccgtc ggtactgatc ccgtcatcaa
     1501 tgaaccggac tgccacgccc tgagcgtcaa attcctttat cagttggatc atatcggcag
     1561 tgtcgcggcc aagacggtcg agcttcttaa ccagaatgac atcaccttcc tccaccttca
     1621 tcctcagcaa atccagccct tcccggtctg ttgggcactg ttgcaaatag tcggtggtga
     1681 taaacttatc atcccctttt gctgatggag ctgcacatga acccattcaa aggccggcat
     1741 tttcagcgtg acatcattct gtgggccgta cgctggtact gcaaatacgg catcagttac
     1801 cgtgagctgc aggagatgct ggctgaacgc ggagtgaatg tcgatcactc cacgatttac
     1861 cgctgggttc agcgttatgc gcctgaaatg gaaaaacggc tgcgctggta ctggcgtaac
     1921 ccttccgatc tttgcccgtg gcacatggat gaaacctacg tgaaggtcaa tggccgctgg
     1981 gcgtatctgt accgggccgt cgacagccgg
//
LOCUS       DAZPJR010000324         2009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPJR010000324 DAZPJR010000000
VERSION     DAZPJR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.330"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..854
                     /gene="espL1"
                     /locus_tag="ACHVB7_004465"
                     /pseudo
     CDS             1..854
                     /gene="espL1"
                     /locus_tag="ACHVB7_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1026..1133
                     /gene="yniD"
                     /locus_tag="ACHVB7_004466"
     CDS             1026..1133
                     /gene="yniD"
                     /locus_tag="ACHVB7_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR1916241.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1186..1944)
                     /locus_tag="ACHVB7_004467"
     CDS             complement(1186..1944)
                     /locus_tag="ACHVB7_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR1916242.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          620 a          452 c          408 g          529 t
ORIGIN      
        1 acgaatgcca tttgccccta tacatccatc ggcaatgttg ttaataatgg ccacaggcca
       61 gactcaatag cttattacac tattcaaaca gttgcccata ctccctgaaa aagaaatcat
      121 tgaaataata actgcgcaga atagcgttgg tacacctgct ttatttctgg ctatgatgaa
      181 cggacaaact gacaacgtaa aaatatttat gcaagaaatt cagtcactgg tatacaatca
      241 tatcattcat gaagataatc tggttaaatt actgcaaact aaaagtgcta acgaaacacc
      301 tggactctat atctccatgt tgtatggatt cgatgaaata atcgatatct ttctgaatgc
      361 attaaccact ccaatagcac aagaactttt aaacaaaaaa aatggtgatg agtattttag
      421 ccatgaaaat acatgatggt gagccaggat tatacgccgc aatggaaaat aatcaccctt
      481 tgtgtgtcac acggttcctc tctaaaatta atggcatcgc ctttaaatac aagttgagca
      541 aagctaacat catggattta ttaaaaggag ctacagcaca gggaacccct gcattataca
      601 tcgccatgag caagggtaat gaagacgtcg tgttatctta tatatcgacg ctgggtgctt
      661 ttgcaaaaaa acattctttt agtcaacatc agttatttac actattggcc gctaaaaatc
      721 atgacaacat gtcagctgtt catatagcca ttcatcataa gcattataaa actgtagaaa
      781 catattatgc tgctataaat gtcatcagcc aaagcctgag ttttagtgct gatgaaataa
      841 agacgtattt ataaccagct atttacgata tatatcgata cataatttta tttttttcat
      901 aaaacgcaga aacagtgacc atgtgcacaa cgccacccga cggctgtaat aacaaaaaat
      961 cccttgatgc ctatcccttt tgttacactc cgttatcacg cacaagagat atgcaggaca
     1021 ctggtatgcc gactaaacgc tttgataaaa aacactggaa gatggtggtg gtgctactgg
     1081 caatctgtgg cgctatgttg ttgctacgtt gggcagcaat gatttggggc tgagcagtga
     1141 caaacgaaga cagccagacc gcataacacg gtccggcaga taattttaca tgctgtagcc
     1201 cagcgataag gttgtccgac gatcggtatg ttccggtgca gactctggag gttcagagtt
     1261 ccatgtgacg ttgtaagcca ccttcagtcc aaaatgttca ttaatagcaa catttaatgc
     1321 gctttcggag ttcagcgttg tatcttccgc gccaaagacc gaaacaccct gcgtaaattt
     1381 agcgttgtca gtcaactgcc atgcataagc cccggaggca tagcccagcg gctgggtttc
     1441 actggcattg tcggtatatt tgtcgtaacg cacacctggg ccgaattcaa agcggaaact
     1501 gtgtaccggg ccattcaaaa actggcgacc ataacccgcg gtcaacacat cgcgctctcg
     1561 atagccgtta taacggtctg tcagccagct tgcctgacca aataaatagt cataatcagt
     1621 taaattaaaa cggctacgcc cgcccgccgc atatttttct gaagaacgct catcattaga
     1681 agaagtatta ctggcgttcc cccacagcga ccaggcagtg gtttgtccat accaggtcat
     1741 ggtggtgtca gccgtaagtg aggagctttt cgtattgcct gattgtgcaa gatatcctgc
     1801 gttcagatta ccttcgaaag gttttttagc gctggcagga tcgtccatga cagtaaaaac
     1861 ggaatcatcg gcagctgcat tcagtgacgc aaacatgccc cccgccaaca taacgatggc
     1921 aggaactgtc ttcaaaagct tcatttatca agagtccgta caacaaaaaa agagaccatc
     1981 gcggtcccgg aaactttctt aaggatcaa
//
LOCUS       DAZPJR010000325         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPJR010000325 DAZPJR010000000
VERSION     DAZPJR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.331"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHVB7_004468"
     CDS             <1..389
                     /locus_tag="ACHVB7_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916243.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHVB7_004469"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHVB7_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR1916244.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHVB7_004470"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHVB7_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR1916245.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHVB7_004471"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHVB7_004471"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHVB7_004472"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHVB7_004472"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZPJR010000326         1996 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPJR010000326 DAZPJR010000000
VERSION     DAZPJR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.332"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..689
                     /locus_tag="ACHVB7_004473"
     CDS             <1..689
                     /locus_tag="ACHVB7_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1916246.1"
                     /translation="SQKDVFMTYRTTGLATYRNYDFFGLIEGLNFAAQYQGKNERTDN
                     SHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAE
                     LWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAY
                     LKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDN
                     IVATGITYQF"
     gene            853..1335
                     /locus_tag="ACHVB7_004474"
                     /pseudo
     CDS             853..1335
                     /locus_tag="ACHVB7_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1450..>1996
                     /locus_tag="ACHVB7_004475"
     CDS             1450..>1996
                     /locus_tag="ACHVB7_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1916247.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          668 a          412 c          344 g          572 t
ORIGIN      
        1 ggagtcagaa agatgtcttc atgacatacc gtaccaccgg tctagcaacc tatcgcaact
       61 acgatttctt tggcttaatt gaagggctga actttgccgc gcaatatcaa ggcaaaaatg
      121 aacgtactga caacagtcat ctttatggtg ctgactacac gcgtgccaac ggtgacggtt
      181 tcggtatctc ctcaacttat gtttatgatg gctttggtat cggtgcggta tataccaaat
      241 ccgatcggac aaatgcgcag gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga
      301 atgcagaact atgggccaca ggtataaagt atgatgccaa caacatctac tttgcagcta
      361 attacgctga aacattaaac atgaccacct atggggatgg ttatatctct aacaaagcac
      421 aaagttttga agtggtggcg caatatcaat tcgacttcgg cttgcgccca tcactcgctt
      481 acctgaaatc gaaaggcaga gatctgggcc gctacggcga ccaggacatg attgagtata
      541 tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt gattataaaa
      601 tcaacctgat tgatgaaagc gactttaccc gtgccgtaga tattcggacc gataacatcg
      661 tcgctacggg cattacctat cagttctaac gtcctctcct gaccagccct tctctgggct
      721 ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa tttttgatat
      781 ttctggtcat tcacctcaat taatattatt ccatatcctc cggggacatc ttactataat
      841 atttattgat gagtgattat gaccaatatt aatacagctt gtgtaaaaaa taatgccagt
      901 tatcaactta ataacgcatt acctaacaaa gaaaccatct ccagcaattt ttgtgaacga
      961 ctagaacaat ggggtaataa atcggttaat aatggtgaag aaagagcaat tgccgtggag
     1021 cgaattaaag aagcttacaa ttcgaacatg gcatctcttg atttgtcata tcttgatttg
     1081 agtgaattac cgcctatccc ctcgacagtt aatacgttaa atctggaaaa caattgtcta
     1141 acttgtcttg acttagctga caatgccagt ctcgttaata tcaacctcag cgttaataaa
     1201 attaacacga taacctttcc aaaagaatca aaactggaaa acatttatat tgatcataac
     1261 aatctggaaa gtttagattt aaaaaatcag cattcattgg ttaatctgga agcgcaaaat
     1321 aacaaactga caaaaattaa tatttctgat agttataaac tgaaatttct taatcttgat
     1381 tataacaagc tagcatcgct ggatgtctcc cggcaagaat ctctgattga gttaagtgcc
     1441 catcacaata tgatcactga ccttatatta cacaatcatc ccaggatgaa aacaatcact
     1501 ttaaacgaca accatattgc acatttaaac gccaaaaaca ctacaaaact ggaatattta
     1561 aacttaagca ataacaattt actgccaacc aatgacattg atcaactaat atcatcaaaa
     1621 catctttggc atgtattagt taacggcatc aacaatgatc cacttgcaca aatgcagtac
     1681 tggactgcag taagaaatat aattgatgac actaatgaag tgaccattga tttatcagga
     1741 cttaatttaa ccactcaacc accagggctg caaaacttca catctatcaa tcttgataat
     1801 aaccaactca cacattttga tgcaaccaac tacgatagac tcgtaaagct aagtctgaat
     1861 agtaatgctc ttgagtcaat aaattttcct caaggcagaa atgtaagtat tacacatata
     1921 tctatgaata ataatgctct cagaaatatt gatatagata ggctttcatc agttacttat
     1981 tttagtgcgg cacata
//
LOCUS       DAZPJR010000327         1988 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPJR010000327 DAZPJR010000000
VERSION     DAZPJR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.333"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(110..580)
                     /gene="argR"
                     /locus_tag="ACHVB7_004476"
     CDS             complement(110..580)
                     /gene="argR"
                     /locus_tag="ACHVB7_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR1916248.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            1015..1953
                     /gene="mdh"
                     /locus_tag="ACHVB7_004477"
     CDS             1015..1953
                     /gene="mdh"
                     /locus_tag="ACHVB7_004477"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1916249.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT          516 a          435 c          514 g          523 t
ORIGIN      
        1 gatatgtcgc ggtaaaacgc actattcgtt aaattaatcg caggggaaag cagggggttg
       61 agaggaataa gcaacatttt ccccgccgcc agaagcgacg gggcagagat taaagctcct
      121 ggtcgaacag ctctaaaatc gcttcgtaca ggtctttgac ggtgaaaccg ttagcagggg
      181 tggtaaagat ggtgtcatcg ccagcgatgg tgcccagaat accttctgct ttgcccagtg
      241 agtccagcag gcgagcaatt aactgcgccg cgccagggct ggtatgaatc acgacaactg
      301 catcgttgta gtcgatatcc agcaccagat tcttcaatgg actggaggtg gttggtacac
      361 ccagttcagc tggcaggcag taaaccattt ccattttggc attgcgtgta cgtacagcac
      421 caaacttggt caacatccgc gagactttag actgattaat attgtcaaag ccttgctcct
      481 gcaacgcggc gacgatttcg ccctgggagc taaatttctc ttctttaagt aatgctttaa
      541 atgctttaac tagttcttct tgcttagccg agcttcgcat aagtcacccg atatggtggt
      601 tgatacaaca ttattgtgca tacagatgaa tttttatgca aacagtcagc cctgaagaag
      661 gctgaaataa tgttatgaaa gagcgggatt ttatcaaatt tcgttattga gaaacatgcc
      721 tgcgtcacgg catgcaaatt ctgtttaaaa gtaaattaat tgttatcaaa ttgatgttgt
      781 tttggctgaa cggtagggta tattgtcacc acctgttgga atgttgcgct aatgcataag
      841 cgactgttaa ttacgtaagt taggttcctg attacggcaa ttaaatgcat aaacgctaaa
      901 cttgcgtgac tacacattct tgagatgtgg tcattgtaaa cggcaatttt gtggattaag
      961 gtcgcggcag cggagcaaca tatcttagtt tatcaatata ataaggagtt taggatgaaa
     1021 gtcgcagtcc tcggcgctgc tggcggtatt ggccaggcgc ttgcactact gttaaaaacc
     1081 caactgcctt caggttcaga actctctctg tatgatatcg ctccagtgac tcccggtgtg
     1141 gctgtcgatc tgagccatat ccctactgct gtgaaaatca aaggtttttc tggtgaagat
     1201 gcgactccgg cgctggaagg cgcagatgtc gttcttatct ctgcaggtgt agcgcgtaaa
     1261 ccgggtatgg atcgttccga cctgtttaac gttaacgccg gcatcgtgaa aaacctggta
     1321 cagcaagttg cgaaaacctg cccgaaagcg tgcattggta ttatcactaa cccggttaac
     1381 accacagttg cgattgctgc tgaagtgctg aaaaaagccg gtgtttatga caaaaacaaa
     1441 ctgttcggcg ttaccacgct ggacatcatt cgttccaaca cttttgttgc ggaactgaaa
     1501 ggcaaacagc caggcgaagt tgaagtgccg gttattggcg gtcactctgg tgttaccatt
     1561 ctgccgctgc tgtcacaggt tcctggcgtt agttttaccg agcaggaagt ggctgatctg
     1621 accaaacgca tccagaacgc gggtactgaa gtggttgaag cgaaagccgg tggcgggtct
     1681 gcaaccctgt ctatgggcca ggcagctgca cgttttggtc tgtctctggt tcgtgcactg
     1741 cagggcgaac aaggcgttgt cgaatgtgcc tacgttgaag gcgatggtca gtacgcccgt
     1801 ttcttctctc aaccgctgct gctgggtaaa aacggcgtgg aagagcgtaa atctatcggt
     1861 accctgagcg catttgaaca gaacgcgctg gaaggtatgc tggatacact caagaaagat
     1921 atcgccttgg gcgaagagtt cgttaataag taattgatta gcgaataata aaaaaccgga
     1981 gcacagac
//
LOCUS       DAZPJR010000328         1973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPJR010000328 DAZPJR010000000
VERSION     DAZPJR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.334"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..765)
                     /locus_tag="ACHVB7_004478"
     CDS             complement(<1..765)
                     /locus_tag="ACHVB7_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1916250.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTT"
     gene            773..1274
                     /gene="tnpA"
                     /locus_tag="ACHVB7_004479"
                     /pseudo
     CDS             773..1274
                     /gene="tnpA"
                     /locus_tag="ACHVB7_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1292..1960)
                     /locus_tag="ACHVB7_004480"
                     /pseudo
     CDS             complement(1292..1960)
                     /locus_tag="ACHVB7_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          487 a          469 c          459 g          558 t
ORIGIN      
        1 cgttgtgact ttgtgaaggt agtccctgcg gatatttgcg atacaggaat gcagtcgctg
       61 tattttgcgt ttctgcttct gccagttgtt gctgaatttg accttgcggc ttaactgtcg
      121 ctgaagtctc gccagcgtct tctggttttt ctgaaaactg tttacaggct caaagactgt
      181 gccatctgac agcgtggcga gtttagccac gccagcatcc agcccgacca ttgatgctga
      241 agggtgagcc ggagtggata cttcactttc tgtctgaata ctgatgtacc acgtaccgca
      301 ggactggctg acagtgacat ttttcacaac acccgtgacc tgacggctat tccggtagcg
      361 catccagccc agtttcggca gaaaaatacg gctgttttcc tgatcgagct taacgccctg
      421 cgggtagcgg aatacatcat tctgtccccg ctttttgaat cggggaaaag cagcccgctt
      481 ccggaagaag tttttgtaag cccgctcaag gtctttcagt gactgttgca atggctgtga
      541 gggagaatct ttaagccatt gcgtttcagt ggcatttttc cactcaacca gccaggaagc
      601 cattttcccg taagggatgt atttattacc ggcctcatga ttctcattct gacgtgccag
      661 agcacgattg aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac actcctgttg
      721 accacctggt cttaactgga atttaaatgc ctgtaatcgt ttcacaggtc aaatgatact
      781 ttggtctgtg aaaaaagaaa ccgatatccg gcgtggaagg cattgtgttt tcctgatgca
      841 tgttcacctg gtctttgtca ctagataccg acgccagatt ttttgacgcg acagaaaaac
      901 tacgcactta cttttcaaat gtatgtgctt attttgaagc tgaactggtt gaaatggatg
      961 gcgaaccaga tcacgtccat ttgttaatta actaacctcc caaactggcg atatccagtc
     1021 tggtaaacag cctcaaaggc gtatcgggta ggttactgcg acgagatcga ccagatattg
     1081 cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc agtagctgcg
     1141 gaggtgtgcc aatatccgtc atccgccaat acattgaaca acagcaaaca cctggtcagg
     1201 tggaaaaccg cgccttatat ccccgccctg aaggatgggg ttttacggcg caccggataa
     1261 aaagcgccat ctaacgatgg cgcgtctgga actacttttt gttaacgtca aacatggtgg
     1321 catcggtcgc catgtcatca ataacttgtt taatattttc gaaagccatt ggcgtacttt
     1381 cgttattcag gtctttacct tcgccctgac gaacgacttt gataaccggt ttattagtcg
     1441 ctgcatcaat cagctcacct tcaaaataga gtcgagtatc catggtacgg tggcctgtag
     1501 ccatttgcgt ccccgcaacc actaatgcaa caggaaccac ttcatagaat tgcagccctt
     1561 ctttgctggt atctacaccg gtaatggccc cacggaaaat cagactacgc ggcccagcgg
     1621 tggtaaccag tggtttacgc tgcgctatcg cttctttcat ttcggtgttg gtatagttca
     1681 aaattttatc cagaactttc tgccctacct aggtagaagg ttttggcacc ggataataag
     1741 tgattgggtt ccagacgatg ctgtcatatt tgctttgatc aaaactcggg tctacccaac
     1801 gtaaaacagg tttacctgtt gctgaggttg tttcttttaa atcagagtaa ttgtttaaaa
     1861 aaccagaata tttatctggc tgggtgattt tagaggcaca accagacaga gccaacaagc
     1921 cgcataaagc tgcaactttc gcaaatgatg tggtacgcat gaatagtttc ctg
//
LOCUS       DAZPJR010000329         1953 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPJR010000329 DAZPJR010000000
VERSION     DAZPJR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.335"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..677
                     /gene="traX"
                     /locus_tag="ACHVB7_004481"
                     /pseudo
     CDS             1..677
                     /gene="traX"
                     /locus_tag="ACHVB7_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            732..1292
                     /locus_tag="ACHVB7_004482"
     CDS             732..1292
                     /locus_tag="ACHVB7_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1916251.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            1422..1634
                     /locus_tag="ACHVB7_004483"
     CDS             1422..1634
                     /locus_tag="ACHVB7_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1916252.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          473 a          452 c          595 g          433 t
ORIGIN      
        1 atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac tggtgctgat
       61 ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt ttctggccgg
      121 gcgaggagcc tttccgcttt ttgccctggt gtgggggctg aatctgtccc gtcatgcgca
      181 tatccggcaa ccggccatta accggctgtg gggatgggga attattgccc agttcgcata
      241 ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg cagtggcggc
      301 acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg ccattcttct
      361 gatggccctg ttgggggcct ttgtccggca ccagttacgg cattgccggg ctgctgatgc
      421 tggcagtcag ccaccgactg taccgggcgg aagacagagc ggaacgtctg gcgctggtcg
      481 cctgcctgct ggctgttatt ccggcgctta accttgccac cagtgatgca gcggcggtgg
      541 ccggtcttgt gatgacggtg ctgaccgttg gtctggtgtc gtgtgcagga aaatcattac
      601 cccgtttctg gcccggggat tttttcccgg tattctacgc ctgtcatctg gctgtgctgg
      661 gcgttctggc gctgtgacgg gtgtggtatc tttgaccgta acaggatgat tcgtcagagg
      721 cagaacacag catgacagag cagaagcgac cggtactgac actgaagcgg aaaacggaag
      781 gggagacgcc tgtccgcagc cggaaaacca tcatcaatgt caccacgcca ccaaagtgga
      841 aggtgaaaaa gcagaagctg gccgagaaag cagcccggga agcagagctg gcggcaaaaa
      901 aagcgcaggc cagacaggca ctgtccattt atctgaacct gcccacgctg gatgaggccg
      961 tgaataccct gaaaccctgg tggccgggat tatttgacgg tgacaccccc cggcttctgg
     1021 cctgcggtat ccgggacgtg ttactggaag acgtggcgca gcggaatatc ccgctctcgc
     1081 ataaaaaact gcgcagggcg ctgaaggcca tcacccgttc agaaagctat ctgtgtgcca
     1141 tgaaagccgg tgcctgccgg tatgacacgg aagggtatgt gacggagcat atttctcagg
     1201 aggaagaagc gtatgcggca gcgcgtctgg ataaaatccg ccgccagaac cggataaagg
     1261 cagaacttca ggccgtgctt gatgagaaat aaaaaaaagc ctccatcaga gaggaggcag
     1321 ggaaataagg cttaaaagga aataacttcc gcttccgaaa ggaagccagt ccactgaatt
     1381 atttatcaca ataatactga caactaaagg gggacaagaa aatgaatgga ttcagaaaca
     1441 gttcacggaa cggccaggtc tggcgttacc agcgtgccgg cgggcgggcc gttattctgg
     1501 aagtcagtgg acgctggatg gaagcggcag aagcatggcg acgggctgcc tgtgttgcgc
     1561 ccaggacaga ctggcagcag tttgcccgaa aaagggctga acactgccac cggcgctgcc
     1621 ggggaagggt gtaacgaaaa aagcagcctt cattactcca ggagtcagaa ggctgaaagg
     1681 agtttatgag atttgctttt tttaatatcc ggtcgggcag tatgaccgga cagttctgag
     1741 tagcacagag ttttttccgt attaagtcgt gattttatat tttgtgatta atttcacaaa
     1801 ataaggtgtt attcagtgtg tgctgcaata ttcaggatgc ctgaaataca gatgctattt
     1861 atctgatatg gagaataaca tgaggaaata gtaaggcagt atgactttcc gtgagacaga
     1921 tgctggccaa cgtgggatat ttaacgcggg tta
//
LOCUS       DAZPJR010000330         1939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPJR010000330 DAZPJR010000000
VERSION     DAZPJR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.336"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHVB7_004484"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHVB7_004484"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR1916253.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHVB7_004485"
     CDS             1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHVB7_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR1916254.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSV"
BASE COUNT          510 a          451 c          456 g          522 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgt
//
LOCUS       DAZPJR010000331         1918 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPJR010000331 DAZPJR010000000
VERSION     DAZPJR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.337"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            93..1265
                     /gene="istA"
                     /locus_tag="ACHVB7_004486"
     CDS             93..1265
                     /gene="istA"
                     /locus_tag="ACHVB7_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR1916255.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1265..>1918
                     /gene="istB"
                     /locus_tag="ACHVB7_004487"
     CDS             1265..>1918
                     /gene="istB"
                     /locus_tag="ACHVB7_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR1916256.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     "
BASE COUNT          487 a          472 c          546 g          413 t
ORIGIN      
        1 cagtgatata agacggtaat tcgccatttg gattgtccgc tccacccaac atgttgtttc
       61 cttaaggttc tcacaccaga aaggacatca acatgctgag cagagaggac ttttacatga
      121 taaagcaaat gcgccagcag ggcgcgtaca tcgtcgatat tgcgactcag attggttgct
      181 ctgaacggac ggtcagacgc tacctcaaat accctgaacc gccagccaga aagacccgcc
      241 acaaaatggt taagctgaaa ccgtttatgg attacatcga catgcgcctg gcagagaatg
      301 tctggaatag cgaggttatc ttcgcggaga ttaaggcaat gggttatacg ggcggacgtt
      361 ccatgctgcg ttactacatc cagcccaaac gtaaaatgcg tccgtcaaaa agaacagttc
      421 gcttcgaaac tcagcctgga taccagctcc agcatgactg gggcgaagtt gaggtggagg
      481 ttgccgggca acggtgcaaa gttaactttg cggttaatac gctggggttc tcccgcagct
      541 tccatgtctt cgccgcacca aaacaggatg ctgagcatac ctacgaatca ctggttcgcg
      601 ccttccgcta cttcggtggt tgtgtgaaaa cggtgctggt tgataaccag aaggctgcgg
      661 tgctgaagaa taacaacggg aaagtcgtgt tcaactccgg attcctgttg ctggctgacc
      721 actataactt cctgccacgg gcatgccgtc cacgcagggc cagaacaaaa ggtaaggttg
      781 agcggatggt gaaatacctc aaggagaact tcttcgtccg gtaccgcagg ttcgacagct
      841 tcactcatgt caatcaacaa ctggagcaat ggatagccga tgtggctgac aaacgggagc
      901 ttcgccagtt caaagaaacg ccggaacagc gcttcgcgct ggagcaggaa catctgcagc
      961 cgttaccgga tacagacttc gataccagtt acttcgatat ccgccatgtg tcctgggaca
     1021 gctatatcga ggttggtggt aatcgttaca gcgttcccga agcgctgtgt ggtcagccgg
     1081 tatcgatacg aatatcgctg gatgacgagt tgcggatcta cagtaatgag aaactggtgg
     1141 cctcacatcg cctctgttca gcatcgtctg gctggcagac agtgccggag catcacgccc
     1201 cgctctggca gcaggtcagt caggtggaac atcgaccact gagtgcctat gaggagctgt
     1261 tgtgatgcat gaactggaag tcctgctgag tcgcctgaaa atggagcatc tgagttatca
     1321 cgttgaaagc ctgctggaac aggcagctaa aaaagagctg aactaccggg agttcctgtg
     1381 catggcgcta cagcaggaat ggaacggcag gcatcagcgc ggtatggagt ccaggctgaa
     1441 gcaggctcgt ctgccgtggg tcaaaacgct ggagcagttc gactttacct tccagccggg
     1501 catcgaccgt aaggttgtcc gggaactggc tggtctggcg ttcgtggagc gcagcgaaaa
     1561 cgtgatcctg ctgggccctc ctggtgtcgg aaaaactcat ctggccatag ctcttggcgt
     1621 gaaagcggtg gatgcgggac atcgggtact gtttatgcca ctggacagac tgatcgcgac
     1681 actgatgaaa gcgaaacagg aaaaccggct ggagcgtcag ctacagcaac tgagttatgc
     1741 ccgggtgttg atcctggatg aaataggcta tctgccgatg aacagagagg aagccagtct
     1801 gttcttccgg ctactgaacc gtcgatatga aaaagcgagc atcatactga cgtcaaacaa
     1861 agggttcgca gactggggag aaatgttcgg agatcacgta ctggcaacag cgatcctg
//
LOCUS       DAZPJR010000332         1899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPJR010000332 DAZPJR010000000
VERSION     DAZPJR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.338"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(89..643)
                     /locus_tag="ACHVB7_004488"
     CDS             complement(89..643)
                     /locus_tag="ACHVB7_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1916257.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="ACHVB7_004489"
     CDS             complement(640..930)
                     /locus_tag="ACHVB7_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1916258.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="ACHVB7_004490"
     CDS             complement(930..1529)
                     /locus_tag="ACHVB7_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1916259.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="ACHVB7_004491"
     CDS             complement(1601..1813)
                     /locus_tag="ACHVB7_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916260.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          472 a          501 c          387 g          539 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
     1861 aaaaggtgat gctgccaact tactgattta gtgtatgat
//
LOCUS       DAZPJR010000333         1876 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPJR010000333 DAZPJR010000000
VERSION     DAZPJR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.339"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(51..185)
                     /locus_tag="ACHVB7_004492"
                     /pseudo
     CDS             complement(51..185)
                     /locus_tag="ACHVB7_004492"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(198..1019)
                     /locus_tag="ACHVB7_004493"
     CDS             complement(198..1019)
                     /locus_tag="ACHVB7_004493"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1916261.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNESILLNGKTIRKLMKELGISSLIRRKKYRTYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1008..1814
                     /locus_tag="ACHVB7_004494"
                     /pseudo
     CDS             1008..1814
                     /locus_tag="ACHVB7_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1288..1404
                     /locus_tag="ACHVB7_004494"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          470 a          414 c          453 g          539 t
ORIGIN      
        1 acgtaggtgc ccaccttttc aaatgatctg cgccccaaat cttggacatc ctatgaaaaa
       61 tgtttaagtc gatattgaac cggactcatg ccatcgagtt tctctttaat gcgatggtga
      121 ttgtagtatt caatgtaatc aattatatct tgctttagat gatgtaactt attgtattta
      181 tttaggtagt aacattctta ttttaaagta ctaaaaaagc tttccattgc tgcattatct
      241 aagcagttac cacgtcggga catgctttgt gttatgccat tatcactcaa ccaacgttgc
      301 caacgaggca tctgatattg ccacccctga tcactatgca acaagggctt atcgtcttta
      361 ttgagttgtt ctaccgcatc taatagcatg ctctttacca ttgatgctaa tgggcgctca
      421 gtaaggttat atgagactat ttctccatta tacaggtcca tgattggtga taaatagagc
      481 ttcctgccat cgaccttaaa ctctgtgaca tctgttaccc acttttgatt tgggcggtca
      541 gcataaaatt gtctcttcag taaattatta caagttcgcc cctgttctcc acgataggta
      601 cggtacttct tgcgcctgat aagtgagctg atacccagtt ccttcattaa ttttctgatg
      661 gttttaccat taagtaaaat gctctcgtta cggcaggcaa gtctgatgcg tcgataacca
      721 taacgccctt tatgctgatg gtatattctc aatattactt gtttaacaag ttgatatgga
      781 tctggcgcat taaggcgttt cacgtgatag taaaatgtac tacgtggcaa atccgcgata
      841 accagtaatt gtgataatgg atgacattgc cttaactcat taataatgat tactttttct
      901 tttgttccaa tcgtaatttt tcctgagcta aggctttcag cttttttaga taatgaaccg
      961 ccccgggaat cctggagact aaactccctg agaaagaggt aaacaggatg actaaaaata
     1021 ctcgttttcc cccgaagtcc gtcaacgggc agtccgtatg gttctggaaa gtcagggcga
     1081 atatgactca caatgggcgg taatttgttc cattactcca aagattggct gtacaccgga
     1141 gactctgcgt gtctgggttc gccagcatga gcgggatacc aggggcggtg atggagggct
     1201 caccaccgct gaacgtcagc gtctgaaaga gctggagcgt gagaatcgtg aactgcgctg
     1261 cagtaacgat atccttcgcc aggcttccgc ttattttgcg aaggcggagt tcgaccgcct
     1321 ctggaaaaaa taatgccact gctggataag ctgcgtgggc agtacggggt cggaccggta
     1381 tgcagtgaac tgcatattgc cccgtcaacg tattaccact gtcagcaaca ggacatcatc
     1441 ctgataaacg cagtgcccgt gcgcagcacg atgactggct gaagagagag atacagcgcg
     1501 tatacgatga aaatcatcag gtgtacggtg tgcgtaaagt ctggcgccag ttgttacggg
     1561 aaggaatcag ggtggccaga tgtacagtgg cgcgcctcat ggcggttatg ggacttgtcg
     1621 gtgttctccg gggtaaaaag gtccgcacta ccgtcagccg gaaaaccgtt gccgcaggtg
     1681 accgcgtaaa ccgtcagttc gtggcagaac gtcctgacca gctgtgggtg gctgatttta
     1741 cttacgtcag cacatggcag ggcttcgtct atgtggcgtt catcattgat gtgtttgccg
     1801 gatacatcgt gtggtgacga gtctcatcgt ctatggaaac gacattcgtg ctggatgcac
     1861 tggagcaggc gttgtg
//
LOCUS       DAZPJR010000334         1818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPJR010000334 DAZPJR010000000
VERSION     DAZPJR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.340"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHVB7_004495"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHVB7_004495"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR1916262.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1006..1416
                     /locus_tag="ACHVB7_004496"
     CDS             1006..1416
                     /locus_tag="ACHVB7_004496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916263.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(1450..>1818)
                     /locus_tag="ACHVB7_004497"
     CDS             complement(1450..>1818)
                     /locus_tag="ACHVB7_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1916264.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgc
//
LOCUS       DAZPJR010000335         1768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPJR010000335 DAZPJR010000000
VERSION     DAZPJR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.341"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1517
                     /gene="tssI"
                     /locus_tag="ACHVB7_004498"
     CDS             <1..1517
                     /gene="tssI"
                     /locus_tag="ACHVB7_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR1916265.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQ
                     TVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQV
                     GLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKD
                     GSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
BASE COUNT          452 a          425 c          559 g          332 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc
     1021 tggtggaaac ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg
     1081 tggggatcat tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta
     1141 tgaatacctc ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg
     1201 tgggaatggg ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga
     1261 tgaaggaaaa caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct
     1321 gtggtaaggc aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca
     1381 ttcatctgga aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg
     1441 gtgccacaca acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg
     1501 atatgcggca attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga
     1561 tgaaccatat taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc
     1621 agggcgacat gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag
     1681 gtgcgccgac gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg
     1741 tgaacccggt gctgggtgcg aaggtgct
//
LOCUS       DAZPJR010000336         1749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPJR010000336 DAZPJR010000000
VERSION     DAZPJR010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.342"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..972)
                     /locus_tag="ACHVB7_004499"
     CDS             complement(<1..972)
                     /locus_tag="ACHVB7_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1916266.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQ"
     gene            complement(1032..1583)
                     /locus_tag="ACHVB7_004500"
     CDS             complement(1032..1583)
                     /locus_tag="ACHVB7_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR1916267.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
BASE COUNT          510 a          329 c          352 g          558 t
ORIGIN      
        1 ctgtaatgta ttgaataaat tagtaatagc aaatgggcca ggaggaacat tttcttgata
       61 aataatacgg ttgttctgtg agacagtgac ctttgcattc gtttgtgcaa cacccgtgat
      121 ttgcggtgcg taaccacgta gatcaggcgg caacatattc tcatcgcttt tcaacgaaac
      181 gccagaaaaa tggaaagagt cgaaaataga ggattcagta tcgtattgac ctatagttag
      241 ttttgcatta attgctggga ttgggcgaaa caaataaata cgcggaaaat ccaggttgtt
      301 cgcctggctt ttgccattgt taaatgactg atcgtactgg taatcagtac gcaggcgcca
      361 ggccccaaga ttaaccccag cctgcccata ggaacttata ttttgtgaat tatcgccctg
      421 atgtggtgca taatgagagg catataaatt gtagtcaaga aatgccccag caataccatg
      481 atcccaaagt tcaggaggcg tccagtttgc atctttgtat tttaaccatg cctgaggtgc
      541 agatatggat aattgcattt tccctttatc gagataagtt gtaatttgtt tttctttttc
      601 gatagggtaa caaccatcga tcaggttttg ctgagcctct tcaataatct catcagtaaa
      661 gcctaataat gttaattgct ctggagtcag acaaactgca gtttggttat caattaatac
      721 ccattgcagg tccagatgga ttggcgtcct tttattatta ataagagcgt ttaatgagta
      781 tttacctacg ggtaattggc catcagtttc aaattgggaa aggttaacgt tttcacgatc
      841 ttcggcttcg attaaatctt tgttgaattc aacggcataa gctgacgcac aaccaatgat
      901 gagaacggat aatgaacaat aaatattttt aatcaattcg cttattgtgg ttaattttaa
      961 ttttttatac atctgttcca cttatcatta atccgttaat gatgtggccg ggcgaacccg
     1021 gcctaatgtg attactcgta agcaatctta tagtcagcct gagcgtaaac gttgcccggg
     1081 gtagcatctt tgcccgggga ttccatacga gctttgaagt tcagttcctg atagctgtta
     1141 gtattgttga atgtaattac taccggagta cctaaagtca cttcgccgct ctggctatcc
     1201 agaatacgta cgccaacgcc tgtggcacca ccgatttcag tttctttggt gtttttcagc
     1261 aggtcagtct gacccgtcat agtattcgct gtggtgaaag taaccttcgc ttttgaataa
     1321 gtggttccac cctggctcaa catgcagtct tgcaggtgaa tagtgaaagg ctcagccaaa
     1381 gatttctggt cgcttttcag aacggtatct gctacttcac ccagatttac agttaagtct
     1441 tcgtcacctg gtttaattga acatggagca gagataactt cacctttaaa tttaacaatg
     1501 ccgtgacctt cgtcagcaaa tacgttattg gaaagaatca ttgctacgcc agcaacaaga
     1561 agtgactttt taaaagcaga cataatattc ctttatataa atataagtag aaaatgaatg
     1621 attagtaact gttatttaag gattgctaat cattcaaata ttatatttta cttttcgggt
     1681 tttatgaaat tagtttatct aaatatggat taagtaaaac ttaaaaaata cataacaatt
     1741 atattttca
//
LOCUS       DAZPJR010000337         1705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPJR010000337 DAZPJR010000000
VERSION     DAZPJR010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.343"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1138
                     /locus_tag="ACHVB7_004501"
     CDS             <1..1138
                     /locus_tag="ACHVB7_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1916268.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="ACHVB7_004502"
     CDS             1150..1599
                     /locus_tag="ACHVB7_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR1916269.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          513 a          281 c          489 g          422 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttactcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggc
//
LOCUS       DAZPJR010000338         1676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPJR010000338 DAZPJR010000000
VERSION     DAZPJR010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.344"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..889)
                     /locus_tag="ACHVB7_004503"
     CDS             complement(<1..889)
                     /locus_tag="ACHVB7_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1916270.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="ACHVB7_004504"
     CDS             complement(989..1345)
                     /locus_tag="ACHVB7_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR1916271.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="ACHVB7_004505"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHVB7_004505"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          333 c          392 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactac
//
LOCUS       DAZPJR010000339         1667 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPJR010000339 DAZPJR010000000
VERSION     DAZPJR010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.345"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHVB7_004506"
     CDS             complement(<1..105)
                     /locus_tag="ACHVB7_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916272.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGK"
     gene            complement(183..604)
                     /locus_tag="ACHVB7_004507"
                     /pseudo
     CDS             complement(183..604)
                     /locus_tag="ACHVB7_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            complement(588..815)
                     /locus_tag="ACHVB7_004508"
     CDS             complement(588..815)
                     /locus_tag="ACHVB7_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1916273.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYAPKVYDEYRKAKRPWKVIHENQA"
     gene            892..1299
                     /locus_tag="ACHVB7_004509"
     CDS             892..1299
                     /locus_tag="ACHVB7_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1916274.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1503..1658
                     /locus_tag="ACHVB7_004510"
     CDS             1503..1658
                     /locus_tag="ACHVB7_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1916275.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          470 a          436 c          344 g          417 t
ORIGIN      
        1 cttccctaat gcggcatctg gatcaatatt cagaatctca gcaagcctga atatttccgg
       61 cttatccggc gtaataacct cgagctttat ccagtttgaa gccatttgtt ttcaccttgt
      121 aacgctcgca gcgttacatt taactgatac cgaacaaaac aatccggcac gattaatttc
      181 aatcaatgca ctacgacaga atcgccgggc gacccaccac cgctgaaatg tgctttccgg
      241 taaacggcct ggacagcatc atcatgcgca tcaattgccg tactcaacgc ttcctgcgcc
      301 gccagtaatg cacggcgttc cagggtatcg aagatacaga gtcggtgacg cagctcacgc
      361 ggaagaattg ccagaaccgc agggatcagt ttctgaattt tttccctttg cgctttcgtt
      421 tcacctttca accaacggtg atagatattc tgctgattgt tccagtcctt gcctggtacc
      481 agagacaatt cgccgccccc ctgccgcaga tattcttcag taattgcgtt agcgacccac
      541 gcctgccctt tttcggctgc cagggcaaca gcactgattc gatgtgctca tgcctgattt
      601 tcatgaatca ccttccaagg tcgttttgcc ttacgatatt cgtcataaac tttgggggcg
      661 tactgaagtt ccccgccaga tgcctcttgc aggcgcatcg cgcgaccttc aggaaccagt
      721 tccccccatg cagcaatgct tgccagccta actcctgcgg cattggcaag ctttgttttg
      781 ctgccaaaaa acgctatagc atcaattttc aacatatcga gctccttaga ttttcctaag
      841 gaaactagat cgtagagaaa cctaagtcaa gaaaaattag aattccctaa tatgaaaaac
      901 gaaaccttcg gtgctcgcct cttacatagg cgtaaaaaat taaaactgtc tcaggccgca
      961 ttaggtaagc tggtcaaagt ggctcacgta acaatttctc aatgggaaag agatgaaaca
     1021 cagccggcgg ggaaaagatt attcgcactg agccaagcgc ttcagtgctc accgacttgg
     1081 cttctttttg gggatgaaga taaacaacca ggcgaaccga tcccggataa tcagccagcc
     1141 attctgacag aagatcaaaa agagttactt caactgttcg acgcactgcc tgagtcagag
     1201 caaaaggccc tgttgtcaga gatgcgtgct cgagttgaaa atttcaacaa actttttgaa
     1261 gaactactca aagctcgcaa aagaagcgca aacaaataat cccctttttt ctccacaccc
     1321 tgtaataaaa agcacaaact ttcaaacact tgtgtttttt acatcaaaaa aacttaggtt
     1381 tttctacaca aaaatcttga ccgcccacct taggttattc taaatttcat tcatcaagac
     1441 accgcacggt gttctcagca aacagttccg ctaccccggc gttaagggga aatggggtca
     1501 acatgaatac tatcgatctt ggcaacaacg aatctctggt gtacggcgtg ttccccaacc
     1561 aggacggcac attcaccgcc atgacgtata ccaaaagcaa aacgtttaaa accgaagctg
     1621 gcgcacgtcg ttggttggga agacattcag gtgagtaaaa tgaacga
//
LOCUS       DAZPJR010000340         1627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPJR010000340 DAZPJR010000000
VERSION     DAZPJR010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.346"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1104
                     /locus_tag="ACHVB7_004511"
     CDS             <1..1104
                     /locus_tag="ACHVB7_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1916276.1"
                     /translation="LAWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTF
                     TAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTA
                     THNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNF
                     LAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGY
                     VTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPG
                     QVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYA
                     QVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          418 a          352 c          405 g          452 t
ORIGIN      
        1 ctggcgtggg cggtctttaa aatctttcgt attccggtga atcagtggac gctggcgacg
       61 gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt tgttgatgaa ctacaaccac
      121 ccttacactt ttaccgcgca aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg
      181 ggaattgtta ctgaagtcac tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt
      241 tttaagctcg acccggttcg ttaccaggcg cgagttgaca gacttcaggc tgacctgatg
      301 acggcgacgc ataatataaa gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc
      361 acccaggttt cagcggagcg cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc
      421 agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc
      481 ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc aggcgcagat ccagagccag
      541 ctcgacagta tggttaacgg cgagcaatcg cagattgtga gcttaagagc gcaacttact
      601 gaagcaaaat ataaccttga gcagactgtc attcgcgcgc cgagcaatgg ctacgttact
      661 caggtactga tccgcccagg tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc
      721 ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt
      781 ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc tacctgggca ggtgttccac
      841 ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt cttatcaggc gcagggggta
      901 ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc tgggaaccat tgaactggac
      961 cctaacgatg atatcgatgc cttacccgac ggcatttacg cccaggtagc agtttactcc
     1021 gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc taagaatgac cagttggatg
     1081 cattatttgt atttggatca ttgaggggga agggggagat tatggaactt tgctccctgc
     1141 aagggctaat tatttttgca tattacttca ttggctgcga tgatttttct ttaatattca
     1201 ttatgttaat aagttctgga tgagtgtgct ctcattggat tttttatccg tcttgttctg
     1261 ccgcaggatg agactataca tcgtcgagat cgcaatgatg atatgagttg ttcctcctgc
     1321 gacggctcta atcaaaaaag agcaggagtg cttttccatc tattgcgatg atggctttat
     1381 tatctgaagg atgacagtca catttaaaaa atatttaatc tatagatcac aatgtagggt
     1441 catggaatat atgtatacaa ctattttctt cttcactttt aattatcgta ttcattataa
     1501 aactaagtta aaaaaactcc tagagagtga gttgttaata tatttttaaa taaaattaag
     1561 ttatcattca tcgatgcatt ttatagaatc atcaatggcc ataatagtac tattaactct
     1621 caatttt
//
LOCUS       DAZPJR010000341         1620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPJR010000341 DAZPJR010000000
VERSION     DAZPJR010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.347"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..202)
                     /locus_tag="ACHVB7_004512"
     CDS             complement(<1..202)
                     /locus_tag="ACHVB7_004512"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1916277.1"
                     /translation="MAEPMAQRPICRQNSHRLHDRLGEPQYTDSLHSQGSASSMWMRH
                     VGGHERFRTGDGQLNTQANRYVL"
     gene            complement(162..1620)
                     /locus_tag="ACHVB7_004513"
                     /pseudo
     CDS             complement(162..1620)
                     /locus_tag="ACHVB7_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
BASE COUNT          364 a          465 c          363 g          428 t
ORIGIN      
        1 gcaatacata gcggttagcc tgagtattta gctggccgtc accggtcctg aaacgttcgt
       61 gccccccgac atgacgcatc cacatactgc ttgccgatcc ctgagaatgc agtgaatctg
      121 tatactgcgg ctcacccaga cggtcgtgta agcgatggct attttgccga catatcggcc
      181 gttgcgccat aggctccgcc atgttcggct aacgtcagct tttgcccacc atggatgttc
      241 agcgctttca gcccatagtt aacgtacaaa ccatcacctg gtgcggtcgt gaggcggaat
      301 ccataatcac cctcggcgac caccgttccg ttttgcgcaa tgtgtaacgt tttgtccgct
      361 tccacctcgt cgccctgtaa atcacgtaac gttaatgagc cacccgaccc aataaccgtt
      421 tgcgccttca ccagttgaac gccgacatgg ctatcatcgt gttccagcaa attgagcgtc
      481 gtcagagggt tattcgctat gggatcattc cacggtttag gcacgtcgat aagcacgtcg
      541 cccgtcccgt ttacctgata gttacggcct ttccaggtgt agtcacccgc gccgacaacc
      601 agcgtatcga cgctgatata tccctctgca agcgtcgcag caggaatatc cgtatcgaaa
      661 atgagcgtac cgccattcat ggccagtcca ccaatggatt gctctcccac gtttaccgat
      721 gtggtatttt cactgtcaga ctgcaacatc gcgtgagtaa gcgcagcggt gttgtcgcgt
      781 tccagagtga aggttctgtc tttcagttgg gcaacaccgg cgaattcagt ccctgttgca
      841 tgggtaaagc caaacatctt gtcgtaggat gacagctgca ctcgcatcaa gccatcgcct
      901 ttgagcgcat tggtcagcgt gtaatctcct gcactgttcg ttgatgcgag aatgtcgagc
      961 gtaccggggc ttgcaatggt gacttcactc gaacccatgc ccgttacccc atctgccgta
     1021 tgagacgcaa tggtcaggag gccgtcattg accagcgttt taccgctata agtattccct
     1081 gcattgaaca ccgtatggcc tgcatcatgg atcacttttc cgtctttatc gtcacctgta
     1141 atcagcatat cgacctggta gccggcatca ctgttattag tatgattaaa gacaaaaaca
     1201 ccttcgccag aaccaaactc tactttcgtc gcgttggtta tatacccggc atctgccgcc
     1261 gcctcaccgt gagctgcacc aatgtttagc gttcctacag caggttcaaa aacacctaag
     1321 taaacttctc caccttcgac attcagcgtg ccgctattcg tcagggttaa cgtacccgta
     1381 ccagatgtcc ctacgtacat attgaatgtt tcaagaagag agttctgccc atccaccctc
     1441 acgtcgccct tacttttgtc attgagagct atctgtgtat cacgcgcttt aactatacct
     1501 ccggtggtaa tattcagttc acccgtgcct aatacaccga cctgtagcaa ttgtgcgtta
     1561 gtagatgatg tctttaaatt ccagagactg tccgttgaaa tattaactat ccccttaccg
//
LOCUS       DAZPJR010000342         1560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPJR010000342 DAZPJR010000000
VERSION     DAZPJR010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.348"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            387..1469
                     /locus_tag="ACHVB7_004514"
     CDS             387..1469
                     /locus_tag="ACHVB7_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR1916278.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          487 a          317 c          316 g          440 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcaatgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
//
LOCUS       DAZPJR010000343         1557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPJR010000343 DAZPJR010000000
VERSION     DAZPJR010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.349"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            271..1386
                     /locus_tag="ACHVB7_004515"
                     /pseudo
     CDS             271..1386
                     /locus_tag="ACHVB7_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          614 a          280 c          221 g          442 t
ORIGIN      
        1 aaaaaaatca cttcacacac tatcagagaa cataataact ttttaaaatg agcgcatttt
       61 gatagttgtt aacgatttcc tttacgatac tatcactacc ctttttttat acacaaatat
      121 taccgggtgg agataaaagg gaaatcaaag gtaattatat caggaagata taacgcatta
      181 catttattgt gtgtagaaga aaatccattc ctgttctaat tatccaattt aaacatctta
      241 gcataaaaca aatgatgaat aaggaatttt atgtcaattg actttacccc aggtataata
      301 aatacatatc acggcgatat ttataactgc acaacaaata ccgataatgc taaaacgcca
      361 gacacaccga agtggccttg cgataattgg gaagaacaac aacccattaa ttccacattc
      421 tctggagaag gatatatctc tgatcaatat gatttagtgc aacaccaact tcaacaaatt
      481 aatgcgtgcc acacgaacac tacatataca aatgcagact actcaaaagt tgtggctcaa
      541 cttgtaagtc ttattactaa cattgaaacg ataagttcga cacaacttac gcaacagaca
      601 caatcaatac ttaatcagat taataacatt agatatgaaa aaaacaaaag tgctgaatgt
      661 cgaatcattg ttgtcgctaa tcctaaaccc gacaaggcaa tcataactaa aattagcgta
      721 gaagagggaa tacccataac attcagtgtc caaaccatgt tttcagatac taattttatt
      781 gctgaacaac gagctgactt accaactaat ataaaagata ttcaaagcct gtatcaaaaa
      841 atgacgaagt tatatattga gcacagtgaa aataaaaaca ggatgaaagt ctttgccgga
      901 gccaacttta ttgatttcaa tatgacagga cagaacctgt ccgggttcgt tctaaccttg
      961 tcaaggtttt atttcgaaga tctacttaat ataaatttca ctgacgcaaa tttaggtgac
     1021 actatttttt acataaagaa caccctaccc ccaaattata taaagatgga caatatcttg
     1081 acaaacaaat cgaaggttta ttttcaacat tattaaccat taatgacaac tttttacgag
     1141 caaaagcgga aattgcttcg acaataatta aatttttaga ggccaggata accaatcttt
     1201 catacaatga catattaaaa tatcagcaag aattccaaaa acaatgttat aagcaagtta
     1261 aagcctttac gacactctct cgatacaata aaattcaaac atgggcagaa atgtctgaat
     1321 atcagttcga agtatttcaa tacgagacgc ttaaccccaa aaaaatgtca cacacgcctt
     1381 atttaaagag gccacttcct aatgaaaaag atataaatta tggggtggaa atagaaatac
     1441 catctggcaa aagaattcgc ttatcaaatc attatcagaa tataatacca taatattgat
     1501 tattagcact ttataattat tgaatgaaaa tagattttat gtaattttaa aacaaag
//
LOCUS       DAZPJR010000344         1549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPJR010000344 DAZPJR010000000
VERSION     DAZPJR010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.350"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            351..476
                     /locus_tag="ACHVB7_004516"
     CDS             351..476
                     /locus_tag="ACHVB7_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR1916279.1"
                     /translation="MWLPVLFRFLVDSLAMLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="ACHVB7_004517"
     CDS             complement(921..1397)
                     /locus_tag="ACHVB7_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1916280.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          359 a          405 c          384 g          401 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacacccca catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctatgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAZPJR010000345         1536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPJR010000345 DAZPJR010000000
VERSION     DAZPJR010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.351"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /locus_tag="ACHVB7_004518"
     CDS             complement(<1..1012)
                     /locus_tag="ACHVB7_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR1916281.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGK"
     gene            1267..1513
                     /locus_tag="ACHVB7_004519"
                     /pseudo
     CDS             1267..1513
                     /locus_tag="ACHVB7_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          438 a          353 c          275 g          470 t
ORIGIN      
        1 tttttccccc aaaataaaca gacattggtg catcattata agctaatgct ttacccattt
       61 tacccgcagc taaatcaact gatgtggcag aaccattatc ttcaacaaat gcagttacac
      121 gaccaccaaa gtttgctaac tgaacatcct taccacccac ctgaactttt tcataacctg
      181 tagagttttg tttcaataat gtaaaaccgt tcgtgttggt attattatct tgaccaacaa
      241 aataagtctg cccttccacc tgtacttgat aaacttttgt tcctgcagta ccggtcgcat
      301 cagttgtatc ctttgtatct tgtacattac cggtaccaaa ataagtttca acatcagcat
      361 ttttataatt gctaccgaat gctttagcac ctcccacact accatcagtt gcatttacct
      421 tagttaagtc aggagcggct tcgctcttaa gtgcaaccgc agaggacatt ggaacttttc
      481 ctagtgctac gctaaagtta tcaagcccca aggttttagc atcaatttta tcaagggcaa
      541 tctcaatggt ttcaccatcg ttcgcaccta cttgaatttt catagtctga tcagaagcca
      601 gaacttttac accattaaat tgtgtctggt tagaaacacg atcaatttca ctaaggcgtt
      661 gtgtaatttc atcctgaatt gaattcaggt cggagtcaga gtttgtacca ttagtggcct
      721 gtactgacaa ttcacgaata cgctgtaagt tattgttgat ttcagaaagc gcaccttcag
      781 tggtctgcgc aacagaaata ccatcattcg cgttacggga agcctgagtc agacctttaa
      841 tatttgctgt aaaacggtta gcaatcgcct gacctgctgc gtcatcttta gcactgttaa
      901 tacgcaggcc agaagagaga cgttcaatgg cggagctcag agaagactga gatttgttca
      961 ggttattctg ggtcaacagc gacaggctgt ttgtattaat gacttgtgcc atgatctttt
     1021 ccttatcaat tacaacttga tgttattggg ctgttgccca cggtttctca ccgtacccct
     1081 tgtatcggca cctgaatttc gaactttaga aaattttagt ttctctcctg ttttttctta
     1141 acggctgaaa tatccgtaat atacaactct actctgcgca tcatcttcgt gaatcaacta
     1201 ttaaactttg cgcacaaatt gccgataacc cgcttatcta ctgttttcaa tcaaaaggaa
     1261 ttaggtatgg cttcatttac atctcttggc gttggttcga atctgccgct ggatacattg
     1321 ctaaacaacc tgaccatcgc tgaaaaaaaa cgcttaaacc caattactca gcaacagagt
     1381 gacaataccg cacgtctgac agcctatggc acattaaaaa tgtgcattag aaaaattcca
     1441 gactgcaaat actgcgttaa ataaagccga tctgttcaaa agcactaccg ttaccagtag
     1501 caatgaagac ctttgaggta gcctgagttt aacgga
//
LOCUS       DAZPJR010000346         1495 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPJR010000346 DAZPJR010000000
VERSION     DAZPJR010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.354"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(11..469)
                     /locus_tag="ACHVB7_004520"
     CDS             complement(11..469)
                     /locus_tag="ACHVB7_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR1916282.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            589..1398
                     /gene="proC"
                     /locus_tag="ACHVB7_004521"
     CDS             589..1398
                     /gene="proC"
                     /locus_tag="ACHVB7_004521"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR1916283.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1415..>1495)
                     /locus_tag="ACHVB7_004522"
     CDS             complement(1415..>1495)
                     /locus_tag="ACHVB7_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916284.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT          342 a          419 c          394 g          340 t
ORIGIN      
        1 taaattacat ttagccacga ctacgttgca cttccagcca ccacttctcc agctccgcgg
       61 caaaggcctg gcggtcacgt tgtgaaaggc tatctggtcc gccggtctgg atcccactgg
      121 cacgtaaggt atccataaaa tcgcgcatcg tcaggcgctc acgaatggtc gctggcgtgt
      181 aacgttcgcc gcgcggatta agcgccgctg cgcctttctc gatggcttca gcagccaaag
      241 gtatatctgc ggtgatcacc aaatcgcccg cttcacactg ccggacaatt tcgttatcgg
      301 caacgtcgaa acctgccgcg acgcgcagcg tacgaataaa tcgcgatggc ggcacgcgta
      361 aactctggtt tgctaccagt accagcggca tctgcatacg ttccgccgcg cgatacaaaa
      421 tctctttaat tacattggga cacgcgtcgg catccaccca aattgtcata aagtcatcct
      481 ttgttgggta atcctctatt gtgtcgcgct tttgccttcc ggcatagttc tgtttatgct
      541 tctgccagcg attatcaaaa caatgaattt cacggcagga gtgaggcaat ggaaaagaaa
      601 atcggtttta ttggctgcgg caatatggga aaagccattc tcggcggtct gattgccagc
      661 ggtcaggtgc ttccagggca aatctgggta tacaccccct ccccggataa agtcgccgcc
      721 ctgcatgacc agttcggcat caacgccgca gaatcggcgc aagaagtggc gcaaatcgcc
      781 gacatcattt ttgccgccgt taaacctggc atcatgatta aagtgcttag cgaaatcacc
      841 tccagcctga ataaagactc tctggtcgtt tctattgctg caggtgtcac gctcgaccag
      901 cttgcccgcg cgctgggcca tgaccggaaa attatccgcg ccatgccgaa cactcccgcg
      961 ctggttaatg ccgggatgac ctccgtaacg ccaaacgcgc tggtaacccc cgaagatacc
     1021 gctgatgtgc tgaatatttt ccgctgcttt ggcgaagcgg aagtaattgc tgagccgatg
     1081 atccacccgg tggtcggtgt gagcggttct tcgccagcct acgtatttat gtttatcgaa
     1141 gcgatggccg acgccgccgt gctgggcggg atgccacgcg cccaggcgta taaatttgcc
     1201 gctcaggcgg taatgggttc cgcaaaaatg gtgctggaaa cgggagaaca tccgggggca
     1261 ctgaaagata tggtctgctc accgggaggc accaccattg aagcggtacg cgtactggaa
     1321 gagaaaggct tccgtgccgc agtgatcgaa gcgatgacga agtgtatgga aaaatcagaa
     1381 aaactcagca aatcctgatg actttcgccg gacgtcaggc cgccacttcg gtgcggttac
     1441 gtccggcttt ctttgctttg taaagcgcca aatctgccga tttcaaccac tcacg
//
LOCUS       DAZPJR010000347         1480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPJR010000347 DAZPJR010000000
VERSION     DAZPJR010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.355"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="ACHVB7_004523"
     CDS             complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="ACHVB7_004523"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR1916285.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHAAIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1090..1458)
                     /gene="cadB"
                     /locus_tag="ACHVB7_004524"
                     /pseudo
     CDS             complement(1090..1458)
                     /gene="cadB"
                     /locus_tag="ACHVB7_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          413 a          324 c          370 g          373 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg cagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt
      961 tcttctttaa aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct
     1021 ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa
     1081 tacttttaat taatgtgcgt tagacgcggt gtggttatcc attgagtggc tctggcgctc
     1141 gtgcattttg cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag
     1201 ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac acacaaccca gtacagagca
     1261 gatcaggctg acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga
     1321 gtagaagtac ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc
     1381 agatgcttta ccaccggcag agttcatcag agtgatgagg attatcaggg cagtcatttt
     1441 cactgcagcc agcagcagta atgccgatca gttaaggatc
//
LOCUS       DAZPJR010000348         1461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPJR010000348 DAZPJR010000000
VERSION     DAZPJR010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.356"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            502..957
                     /gene="csgB"
                     /locus_tag="ACHVB7_004525"
     CDS             502..957
                     /gene="csgB"
                     /locus_tag="ACHVB7_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR1916286.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            998..1450
                     /gene="csgA"
                     /locus_tag="ACHVB7_004526"
     CDS             998..1450
                     /gene="csgA"
                     /locus_tag="ACHVB7_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR1916287.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          446 a          276 c          337 g          402 t
ORIGIN      
        1 aatgatagaa aagttgtaca tttggttttt attgcacaat tttaaaaaat catacaaatg
       61 gtgataactt actaataatg catataaaaa atatttcggt gtagtccttt cgtcatgtaa
      121 aaagttgttg ttttttctcc acacctccgt ggacaaattt ttactccaaa aagacgaggt
      181 ttgtcacggc ttgtgcgcaa gacatatcgc agcaatcagc gatgggcaag aagaatgact
      241 gtctggtgat ttttgatagc ggaaacggag atttaaaaga aaacaaaata ttttttgcgt
      301 agataacagc gtatttacgt ggcttttaat actttggtat gaacaaaaaa agaaaaatac
      361 aacgcgcggg tgagttatta aaaatatttc cgcagacata ctttccatcg taacgcagcg
      421 ttaacaaaat acaggttgcg ttaacaacca agttgaaatg atttaatttc ttaaatgtac
      481 gaccaggtcc agggtgacaa catgaaaaac aaattgttat ttatgatgtt aacaatactg
      541 ggtgcgcctg ggattgcagc cgcagcaggt tatgatttag ctaattcaga atataacttc
      601 gcggtaaatg aattgagtaa gtcttcattt aatcaggcag ccataattgg tcaagctggg
      661 actaataata gtactcagtt acggcaggga ggctcaaaac ttttggcggt tgttgcgcaa
      721 gaaggtagta gcaaccgggc aaagattgac cagacaggag attataacct tgcatatatt
      781 gatcaggcgg gcagtgccaa cgatgccagt atttcgcaag gtgcttatgg taatactgcg
      841 atgattatcc agaaaggttc tggtaataaa gcaaatatta cacagtatgg tactcaaaaa
      901 acggcaattg tagtgcagag acagtcgcaa atggctattc gcgtgacaca acgttaattt
      961 ccattcgact tttaaatcaa tccgatgggg gttttacatg aaacttttaa aagtagcagc
     1021 aattgcagca atcgtattct ccggtagcgc tctggcaggt gttgttcctc agtacggcgg
     1081 cggtaaccac ggtggtggcg gtaataatag cggcccaaat tctgagctga acatttacca
     1141 gtacggtggc ggtaactctg cacttgttct gcaaactgat gcccgtaact ctgacttgac
     1201 tattacccag catggcggcg gtaatggtgc agatgttggt cagggctcgg atgacagctc
     1261 aatcgatctg acccaacgtg gcttcggtaa cagcgctact cttgatcagt ggaacggcaa
     1321 aaattctgaa atgacggtta aacagttcgg tggtggcaac ggtgctgcag ttgaccagac
     1381 tgcatctaac tcctccgtca acgtgactca ggttggcttt ggtaacaacg cgaccgctca
     1441 tcagtactaa tacatcattt g
//
LOCUS       DAZPJR010000349         1449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPJR010000349 DAZPJR010000000
VERSION     DAZPJR010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.357"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            10..747
                     /gene="erm(B)"
                     /locus_tag="ACHVB7_004527"
     CDS             10..747
                     /gene="erm(B)"
                     /locus_tag="ACHVB7_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR1916288.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          533 a          254 c          233 g          429 t
ORIGIN      
        1 gtaaattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
       61 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
      121 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
      181 gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc
      241 actttaattc accaagatat tctacagttt caattcccta acaaacagag gtataaaatt
      301 gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc
      361 catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt
      421 caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg
      481 ccagcggaat gctttcatcc taaaccaaga gtaaacagtg tcttaataaa acttacccgc
      541 cataccacag atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg
      601 gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac
      661 gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt
      721 tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt
      781 acacgttact aaagggaatg tagataaatt attaggtata ctactgacag cttccaagga
      841 gctaaagagg tccctagact agcaagaagt acacaagaag ccttaaagat tatagaaaag
      901 cttttccatg aaatgcttaa cattatttaa tgttaacatg tggctttgtg gtaactacca
      961 cgagtggtaa cttgtaacat gaaaaaagtt acaagttaca agtggtttat ttacgaacat
     1021 aaaggctagg aaagcacttg ataagtatat caagtgcttt ttttccgttc gattttacta
     1081 tccaagatac cgcttgacgt acctaatatt ttagtgtgct aagggctttg ctttttctgg
     1141 ttatagttcg ttcaaaaaag accgcaaggg taaatgcacg atcatagatc gcccttgcag
     1201 ctttttaatc tctccacttt tttataacca agcaaaccac caagcacact gaaaaaataa
     1261 ttaggaacta aagcgaagtg cgtatcttgg ataaatcttc actcgatttt aaaagattaa
     1321 gcacccagcc aaagcgccta aaaaaataat ttttttaaag aaaggaagta atttaaatga
     1381 ctacaatttt aagcgactac aataaatcta tcctaattga attactaaac accaatcgcc
     1441 aaattatcg
//
LOCUS       DAZPJR010000350         1431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPJR010000350 DAZPJR010000000
VERSION     DAZPJR010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.358"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHVB7_004528"
     CDS             <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHVB7_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR1916289.1"
                     /translation="SQEKILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLE
                     IAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDV
                     PLNMIRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHVB7_004529"
     CDS             464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHVB7_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR1916290.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          373 t
ORIGIN      
        1 ttcgcaagaa aaaatattac gcattattca caccaaaggt gtgtcattgc ctgatgatcg
       61 cgagtcagtc ttctggctga atattaaaaa tattccgcca tcagcgagca acaaagcgac
      121 gaactcgctg gaaatagcag taaaaacgcg tatcaagctg ttctggcgac cagcaaatat
      181 ccgccttatt cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag ggcgaaatct
      241 gatagcagag aatcccaacc caattcatat cagtgtgatg gatgtcatcg ttgatggcca
      301 tgatgtgccc ctcaatatga tccgtccttt tgaaacgctc acgttacctt tacctgctaa
      361 ttctgcggga ggccaaatga cctggcgatt tattaatgat tacggcgcag tcagtgaccc
      421 gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga cgaccagaat
      481 agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga tggccgaggt
      541 cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg atattcgcca
      601 gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt ttatcaacgg
      661 taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg ctgatctgca
      721 cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag ttgatgcgct
      781 caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac gtattgaagg
      841 ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc cccaaatcta
      901 catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg ggattaacgc
      961 gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt atggttcaca
     1021 agagacagat accagttatc tcaatctgcg caatggactg aatattggac cgtggcggct
     1081 acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact ccattagtac
     1141 ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg atacgtggac
     1201 ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata ctgataacga
     1261 tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg caaagtccaa
     1321 cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtctgccg ttccacaagg
     1381 tgcttttgag atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg a
//
LOCUS       DAZPJR010000351         1428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPJR010000351 DAZPJR010000000
VERSION     DAZPJR010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.359"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHVB7_004530"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHVB7_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1916291.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHVB7_004531"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHVB7_004531"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR1916292.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1428)
                     /locus_tag="ACHVB7_004532"
     CDS             complement(822..>1428)
                     /locus_tag="ACHVB7_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1916293.1"
                     /translation="KGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFC
                     FGKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGAD
                     EEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVP
                     GAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          324 a          397 c          396 g          311 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgccttta
//
LOCUS       DAZPJR010000352         1420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPJR010000352 DAZPJR010000000
VERSION     DAZPJR010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.360"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>1420
                     /gene="rhsC"
                     /locus_tag="ACHVB7_004533"
     CDS             <1..>1420
                     /gene="rhsC"
                     /locus_tag="ACHVB7_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFR1916294.1"
                     /translation="VLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRL
                     SAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAH
                     RHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGL
                     KRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASA
                     FYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATED
                     ATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQL
                     IAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDA
                     AGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHW
                     HYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          421 c          436 g          225 t
ORIGIN      
        1 tggtactgac cacgcaggcg cagcgggcag aagaagcccg gcagcaggcc atttccggcg
       61 ggacggaacc gtccgctttt cctgataccc tgccgggtta caccgaatat ggccgggaca
      121 acggcatccg tctgtctgcc gtgtggctga cgcacgaccc ggaatacccg gagaatttac
      181 ctgccgcgcc gctggtgcgc tatggctgga cgccgcgcgg cgaactggcg gcagtgtatg
      241 accgcagcaa tacacaggtg cgcagcttta cttacgatga taaataccgg ggccggatgg
      301 tggcgcaccg tcacacgggc cggccggaaa tccgttaccg ttacgacagc gacgggcggg
      361 tgacagaaca gctaaacccg gcaggcttaa gctacacgta tcagtatgag aaggaccgca
      421 tcaccatcac cgacagcctg aaccgccgtg aagtgctgca cacgcagggt gaagccgggc
      481 tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt cagtttgatg
      541 cggtgggcag gctcagggca cagacggatg ccgcaggccg gacgactgag tacagcccgg
      601 atgtggtgac gggactcatc acgcgcatca ccacgccgga tggcagggca tcggcgtttt
      661 actataacca ccacagccag ttaacgtcag ccaccgggcc tgacgggctg gaaatgcgcc
      721 gcaaatatga tgaatccggc cgtctgattc aggaaactgc ccctgacggc gatatcatcc
      781 gctaccgtta tgataatcca cacagtgact taccctgcgc aacggaagat gccaccggca
      841 gccggaaaac catgacgtgg agccgttacg gtcagttgct gagcttcacc gactgttccg
      901 gttatgtaac ccgttatgac catgaccgct tcgggcagat gacggcggtg caccgcgagg
      961 aagggctgag tcagtaccgc gcatacgaca gccgtggaca gttaattgcc gtgaaagaca
     1021 cgcagggcca tgaaacgcgg tatgaataca acgccgccgg tgacctgacc accgtcattg
     1081 ccccggacgg cagcagaaac gggacacagt acgatgcgtg gggaaaagct atctgtacca
     1141 cgcagggcgg tctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga
     1201 ccagtgaaaa cggcagccac accaccttcc gttacgatgt actcgacctg ctgatacagg
     1261 aaaccggctt tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc
     1321 gcagcgagga tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcacgc
     1381 accgcaccgt gaatggcgaa accgcagagc ggtggcagta
//
LOCUS       DAZPJR010000353         1393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPJR010000353 DAZPJR010000000
VERSION     DAZPJR010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.361"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..460
                     /locus_tag="ACHVB7_004534"
     CDS             <1..460
                     /locus_tag="ACHVB7_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1916295.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            478..1116
                     /locus_tag="ACHVB7_004535"
     CDS             478..1116
                     /locus_tag="ACHVB7_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1916296.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          445 a          282 c          307 g          359 t
ORIGIN      
        1 attgaatgca atggcgtcgc aacgaaccaa agaggctcgt ggtggagaat tacagatcaa
       61 cgaattactt cccgccagcc tggatgcagc aaaagcccat ggactgaccg aacgcgatgt
      121 ttatgaagca actgactact ataaaacacc acgcgggcaa caaccgggag gtgcgacaaa
      181 aatgttgttc tcacatgcac agaaaacgct ggcctgggat gccttcgcat ttactgaagt
      241 gttactgact cagccggtga tggtagtagt tggtgaaaaa gtcggtgcct tcggtgctta
      301 tcgtgatgga ctggaagtct atggccgggc gatggtttcg caggatcgtc agttagtttc
      361 attacccgat ttctcacatt atgaactgta tgacaaaccg gaagcagttc aggaggcgct
      421 ggaaaaagtt attccttttt tcaacaccca tctgggataa gtttcatgag aaatattatg
      481 aaaaatgtac tgattcttgg tgctggcggt cagatagccc gccatgtgat taatcaactg
      541 gcagataaac aaaccattaa acaaacttta tttgcccgtc agccagcaaa aatccacaaa
      601 ccctatccaa ctaatagcaa gattattatg ggagatgtac tgaatcatgc agcactgaag
      661 caggccatgc agggacagga cgttgtgtat gccaacctta cgggggaaga tttagatatt
      721 caggcaaatt cagtgatcgc tgcgatgaaa gcctgtgatg ttaaacgtct gatctttgtt
      781 ctttccctcg gtatttatga tgaagttcca ggaaaatttg gcgaatggaa taatgcagtt
      841 attggtgagc ccctaaaacc attccgcagg gctgcggatg caattgaggc ttctggactg
      901 gaatatacaa ttcttcgccc tgcctggctg actgatgaag atattattga ttatgagctt
      961 acctcacgca atgaaccatt caaaggtacg atcgtatcac gtaagagtgt cgcagcatta
     1021 atcacagata tcatcgataa acctgagaaa cacattggtg agaatatagg tatcaatcaa
     1081 cccgtgactg atggtgacaa gccattattc atgtaaacca attaaatagt taccatcgta
     1141 atgtactatg tgcagtacga tttattaaac ttattaaggt gaactgatca atccgatgcg
     1201 attaacatgt gtagaaccag taagaataaa atacgccatg tatataacat tacgacttac
     1261 atctaagtca gcataattct aatatctgca catcaataaa aatgcaaaca acttccggca
     1321 aaaaaagtga tacaagatcg atgacatagc gttgaagaaa aggatttgat aatccgcaaa
     1381 atttgcacaa cac
//
LOCUS       DAZPJR010000354         1388 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPJR010000354 DAZPJR010000000
VERSION     DAZPJR010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.362"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            8..964
                     /gene="ldtC"
                     /locus_tag="ACHVB7_004536"
                     /pseudo
     CDS             8..964
                     /gene="ldtC"
                     /locus_tag="ACHVB7_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(1047..1304)
                     /gene="bhsA"
                     /locus_tag="ACHVB7_004537"
                     /gene_synonym="ycfR"
     CDS             complement(1047..1304)
                     /gene="bhsA"
                     /locus_tag="ACHVB7_004537"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR1916297.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT          353 a          348 c          350 g          337 t
ORIGIN      
        1 atttttggtg atgatcaaaa cgcgtttttc tcgctggcta acgtttttta cgttcgccgc
       61 tgccgtggcg ctaccggcaa aagccaacac ctagccgctg ccgccagcga gcagtcgtct
      121 ggttggcgaa aacaaatttc atgtggtgga aaatgacggt ggttctctgg aagccatcgc
      181 caaaaaatat aacgtcggct ttctcgctct gttacaggct aaccccggcg ttgatcctta
      241 cgtaccgcgc gcgggcagcg tgttaacgat cccgttgcaa accctacttc cagacgcgcc
      301 gcgcgaaggc attgtgatca acattgcgga gctgcgtctc tattactacc cgccgggtaa
      361 aaattcggta accgtgtatc caataggtat tggtcagtta ggtggtgaca cgctgacacc
      421 gacaatggtg accaccgttt cagacaaacg tgcaaaccca acctggacgc caacggcaaa
      481 catccgcgcc cgttataaag cacagggaat tgagttgcct acggtagtgc cggctggacc
      541 ggataaccca atgggccatc atgcgattcg tctggcggcc tatggcggcg tttatttgct
      601 tcacggtacg aacgccgatt tcggcattgg catgcgggta agttctggct gtattcgtct
      661 gcgggatgac gatatcaaaa cactctttag ccaggtcacc ccaggcacca aagtgaatat
      721 catcaacact ccgataaaag tctctgccga accaaacggt gcgcgtctgg ttgaagtaca
      781 tcagccgctg tcagagaaga ttgatgacga tccgcagctg ctgccaatta cgctgaatag
      841 cgcaatgcaa tcatttaaag atgcagcaca aactgacgct gaagtgatgc aacatgtgat
      901 ggatgtccgt tccgggatgc cggtggatgt ccgccgtcat caagtgagcc cacaaactct
      961 gtaaatcctg gataaaaaaa atccccgcga cacggcatgt cgcggggaaa aaatcagcag
     1021 tagcaggcat taatgagggt taatgcttat ttataaatta ctgctgttcc atggagggta
     1081 ttcggaccgg ttacagaagt aatacggaaa gattttgcgc ccatctcatc cgctttttgc
     1141 gccagctgct cttccagcga tcccagattt gtccccgcgt tagcactgat agtaccgact
     1201 ttttgttggc cttctggcgt tgactgaact tcgacagccg caaagttggc aaatgacatg
     1261 gagcttaaaa tcgccgcagc gatgagggtt tttacgtttt tcataatagt ggccttatgc
     1321 agatgaatga cgtcgcaaga aattacttaa cgatcgataa ctaaatgata gatgtgatct
     1381 ggatcaca
//
LOCUS       DAZPJR010000355         1381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPJR010000355 DAZPJR010000000
VERSION     DAZPJR010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.363"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHVB7_004538"
     CDS             complement(<1..304)
                     /locus_tag="ACHVB7_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR1916298.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(1125..1337)
                     /locus_tag="ACHVB7_004539"
     CDS             complement(1125..1337)
                     /locus_tag="ACHVB7_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFR1916299.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
BASE COUNT          449 a          288 c          261 g          383 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataatc
      961 tattgattag gttatattaa gaatcaatat ttaaaataca tccattttct tgaataaatc
     1021 aacatattta tttatcaatt gctgttttag ctctgactca tcaagctctc catatttatc
     1081 ctatagataa ttgcgaataa atacagagaa cgccatttat ccttttagca tacctgccgg
     1141 atatttttat ttgcattaat attaattgca tctccaactc cgagcatatc catcattaaa
     1201 aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac aatgtgaata
     1261 gttagtttct tttcagagga aatgaaatag acaagctgac tgaaggggag agtgatataa
     1321 atcaaaaaac ataacatgaa tttaattaat tacgcccatc attgtggata aagttactga
     1381 a
//
LOCUS       DAZPJR010000356         1380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPJR010000356 DAZPJR010000000
VERSION     DAZPJR010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.364"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1249
                     /locus_tag="ACHVB7_004540"
     CDS             <1..1249
                     /locus_tag="ACHVB7_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HFR1916300.1"
                     /translation="YRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVL
                     DSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMA
                     SPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLD
                     EMIVLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYI
                     ENRMYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVD
                     FDSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQ
                     WFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWK
                     KSGPEMASLEAWLEEHEKYRSVA"
     gene            1246..>1380
                     /locus_tag="ACHVB7_004541"
     CDS             1246..>1380
                     /locus_tag="ACHVB7_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HFR1916301.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     P"
BASE COUNT          323 a          355 c          404 g          298 t
ORIGIN      
        1 ctaccgtaac cagctggcgt ttatcgatat tttcttccgg ggaatgaata acaccaacta
       61 caacatggcg gtctgtggca cctccggggc cggtaaaacc gggctgatac agccacttat
      121 ccgcagcgtg ctggactccg ggggctttgc cgtggtgttc gacatggggg atggctacaa
      181 gtccctgtgt gagaacatgg gtggcgtata tctggacggt gaaaccctgc gttttaaccc
      241 gtttgcgaac atcaccgata tcgaccagtc agcggagcgt gtccgtgacc agttgtcagt
      301 gatggccagc cccaacggta acctggatga agtgcatgaa ggtctgctgc tgcaggcggt
      361 cagggcctcc tggctggcca aagagaacag ggcacgtatt gatgacgtgg tggatttcct
      421 gaaaaacgcc agtgacagcg agcagtatgc cgggtcaccg actatccgca gccgtctgga
      481 cgaaatgatt gtgctgcttg accagtacac tgccaacggc acttacggcc agtattttaa
      541 ctctgatgag ccgtccctgc gggatgacgc aaaaatggtg gtgctggagc tgggcggact
      601 ggaagaccgt ccgtcactgc tggttgcggt gatgttctcc ctgattatct acatcgagaa
      661 caggatgtac cgcacgccgc gtaacctcaa gaaactgaac gtcattgatg aaggctggcg
      721 tctgctggac ttcaaaaatc acaaggtcgg tgagtttatt gagaaaggtt accgtaccgc
      781 ccgtcgtcat accggtgctt atatcaccat cacacagaac atcgtcgact ttgactctga
      841 caaggcctcc agtgcggccc gtgcggcatg gggtaactcc tcctacaaaa ttatcctcaa
      901 acagagtgcg aaggagttcg caaaatacaa ccagctgtat ccggaccagt tccagccact
      961 gcagcgcgac atgattggta agtttggtgc ggccaaagac cagtggttca gttccttcct
     1021 gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt gtggacccgt taagccgcgc
     1081 catgtacagc tctgacggcc cggattttga gtttgtgcag cagaaacgta aagaggggct
     1141 gagtattcat gaggcggtgt ggcagctggc gtggaagaag tcagggccgg agatggcttc
     1201 gctggaagcc tggctggaag aacatgagaa atacaggagt gttgcatgac cacaacgcaa
     1261 aaaacgactg atgttacagc cccccgacgc agccactggt ggtggacggt gccgggatgc
     1321 ctggcaatgg tgttactgaa cgcagccgtc agttacggca ttgtcagact gaacgcaccg
//
LOCUS       DAZPJR010000357         1376 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPJR010000357 DAZPJR010000000
VERSION     DAZPJR010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.365"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..983
                     /locus_tag="ACHVB7_004542"
     CDS             <1..983
                     /locus_tag="ACHVB7_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1916302.1"
                     /translation="VVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1376
                     /locus_tag="ACHVB7_004543"
     CDS             998..>1376
                     /locus_tag="ACHVB7_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1916303.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          359 a          315 c          363 g          339 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattag
//
LOCUS       DAZPJR010000358         1341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPJR010000358 DAZPJR010000000
VERSION     DAZPJR010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.366"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHVB7_004544"
     CDS             <1..184
                     /locus_tag="ACHVB7_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916304.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHVB7_004545"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHVB7_004545"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1916305.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZPJR010000359         1331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPJR010000359 DAZPJR010000000
VERSION     DAZPJR010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.367"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            178..849
                     /gene="queE"
                     /locus_tag="ACHVB7_004546"
     CDS             178..849
                     /gene="queE"
                     /locus_tag="ACHVB7_004546"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR1916306.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          369 a          270 c          292 g          400 t
ORIGIN      
        1 gacatgatta aatccatttt ctttattttc ttctgacata atacgttact ccctttaatt
       61 attaacgata tgtttcgcat ttttctttaa atctgcctgt aacagaatgt taataagaca
      121 tgattgttgt ggcaggatct gcaataatta acaattatcc acttcacaga gaatgctatg
      181 cagtacccga ttaacgagat gttccagacc ctgcaaggtg agggttactt taccggcgtc
      241 cccgccattt ttattcgttt acagggatgc ccggttggct gtgcctggtg cgacaccaaa
      301 cacacctggg aaaagcttga ggatcgggaa gtctcccttt tcagcattct ggcgaagacc
      361 aaagagagtg ataagtgggg ggcggcgagc agtgaagact tgttggctgt cattagtcgc
      421 cagggataca ccgcgcggca tgtggtgatt acgggtggtg agccttgcat tcatgatttg
      481 ctaccactga ctgatctgct cgaaaagaac ggttttagct gccagatcga aaccagtggt
      541 actcatgagg tacgctgcac accgaatacc tgggttaccg tatcgccaaa gctgaacatg
      601 cgcggcggct atgacgtgtt gtcacaggca ctggagcgag ccaacgaaat caagcatcca
      661 gtggggcgcg tacgcgatat tgaagcactg gatgaactat tggcaacgct gaccgatgat
      721 aaaccgcgag tcattgcact gcagccgatt agccaaaagg atgatgccac acgtttgtgc
      781 attgaaacct gcattgcgcg taattggcgt ttgtcgatgc aaacacataa atatctaaat
      841 attgcctgat taaacattta taagcgttat aaatgggtgg aacctgtaaa gacttctact
      901 catttatatt gtttgtcgcc tctgaaaact cctccatttt acccatccag ggctaatcat
      961 tagcattctc tacaaattct gtggcattaa tttttcgctg gagtgaaaat tattgcggta
     1021 aagtttggta gattttaatt tgtatagagt tattttaaat atttaccttt ttaatcaatg
     1081 aattaagtac tctttaacat aatggatgtg ttgtttgtgt gatactgtaa agttggtaga
     1141 ttgtgactgg cttaaaaaat cattaattaa taataggtta tgtttagagt gttccccgcg
     1201 ccagcgggga taaaccacca tataacccgt tatctctttc tcaagttttt atattagcag
     1261 tacttgtaat aagcaaaata tccacgtaac acctcatgtt caaaatagtt ctccatgcca
     1321 gagaggttca c
//
LOCUS       DAZPJR010000360         1318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPJR010000360 DAZPJR010000000
VERSION     DAZPJR010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.368"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(241..927)
                     /locus_tag="ACHVB7_004547"
                     /pseudo
     CDS             complement(241..927)
                     /locus_tag="ACHVB7_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          320 a          345 c          328 g          325 t
ORIGIN      
        1 accaacaaaa agaatgcact agcgagtgat gtttaccagc cgatattatg gggtccgctt
       61 ggaaaacgga tctatggtca ctcccgtttt tgcaacaccg attttgacga taagtgggct
      121 tgcttgaatc tatccggagt ctgaatggga tttcattccc gcgccccgat gagttccgcg
      181 cctgatgtac atgtcgaaga aaacggccgg cggggggaat acggcgcgct acctgggtcg
      241 ttatcggggg gtgagggcca atggaacgaa aacgtacgtt aatgtgtgcg tatcagccac
      301 atactgtgcg aaagtttccg gcgtgaagtt cctgtgcgtg tatttcgcaa tgctcttgcc
      361 aatagcccga cattcaggcg gtgaaagcgg aacgggaagc gatgcgttgt acatttcgac
      421 acgctgaatc acggcatcaa gccactgtga gaattcgggc cagccctgac gaatcgccct
      481 gtaagcccat ttgcgcgtca tttcgaacag gtggcagttt cgccccatcc cgtaatgttt
      541 atcgatgcta cggcgcgctg aggcgctcaa atcgagataa tcagccagtt catcgagggt
      601 ataggcttcc tctcgccact ccatcacctg ccattccagg tggaacggat ttttacaaat
      661 cagtccgctg tagttcacat cagcacaaag tttctcacac agcgaacgtt caatcgcagc
      721 ggcgtatttc agcgccttaa ccgagctgtc cggcgcggtt ctcacggcga tattgagcgc
      781 gtagagcagg tgagcatgac cgttcacagg attttttacg gtgatgttcg gcgcgggagc
      841 gttccggtcg ctccagtcaa tcgctgcccc ctcgcgatca acgtcaaaaa ccagccagaa
      901 ctgcgcatga ggctggttct gccggatctg gacatgattt caggggcgtt tgtttttcag
      961 tcgcttacac tacacccaac ggcaaatcct gtcgaacctt cccgaaaacc cgatccattt
     1021 accataactc ccgttgtacg tgccctctca gacgctctct gacgcaaaaa actcaaaaag
     1081 acaagaaaaa acagggtaaa cataaaaaat gcgtcagaat cgtttttagc gcgttgcata
     1141 actatgcacg aaagcgaccc gcgatcggtt ttgtgtgttc tgaataccga acgggtcaaa
     1201 atgacacgtt ttctctccct caatggagcg ttctatacag agatttttgt tcccctatcc
     1261 attattctga ttaccgggtt tccaagctga aaacaggact tttctgcgac cagttttt
//
LOCUS       DAZPJR010000361         1315 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPJR010000361 DAZPJR010000000
VERSION     DAZPJR010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.369"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..185)
                     /locus_tag="ACHVB7_004548"
     CDS             complement(<1..185)
                     /locus_tag="ACHVB7_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1916307.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(230..1216)
                     /locus_tag="ACHVB7_004549"
     CDS             complement(230..1216)
                     /locus_tag="ACHVB7_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR1916308.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          356 a          294 c          269 g          396 t
ORIGIN      
        1 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
       61 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
      121 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
      181 atcattgtaa gcatcttgga aaatcagggc cagagactgg cccggaatat tattgagcag
      241 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
      301 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
      361 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
      421 cggatttctt cgctgctgtt tcgaggattt gtttctgttc tggggttaat ttattccatg
      481 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
      541 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
      601 ctacaccttg ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
      661 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
      721 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
      781 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
      841 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
      901 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
      961 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1021 tacggatcac catattacct tctgacagct gttttacctc tttcgccagc tcttcaaaac
     1081 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
     1141 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1201 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1261 tgtgcttatc agtgctttgt gcgacaaatt ggttaaccta tctgacaact tgtct
//
LOCUS       DAZPJR010000362         1264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPJR010000362 DAZPJR010000000
VERSION     DAZPJR010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.370"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(23..>1264)
                     /locus_tag="ACHVB7_004550"
     CDS             complement(23..>1264)
                     /locus_tag="ACHVB7_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1916309.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYPTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          333 a          298 c          228 g          405 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcaggat gtgtttaacg cttaatgctg accagttgcc atccggtttc ttagtcttgg
      961 atattctgac cttacgctga ttaatggtcg ggtaaccttg ttcgtttagc catttacaga
     1021 tagcaaacat cgacatacca gaaagacgct tgtcatagat gatattcacc agtgacgctt
     1081 tatcttcatc aacgatgtag tatgaaccat cttctgatag ctctaaccat ccgggggctt
     1141 gcttacccat aggcttctta agttctcttg caagctgtgt tttatggttc cagacctgag
     1201 ctatacgctt ttgcttaaca agagattctt ggtgtgctct ctcagctatg atgatgtgct
     1261 taat
//
LOCUS       DAZPJR010000363         1264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPJR010000363 DAZPJR010000000
VERSION     DAZPJR010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.371"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..301
                     /gene="ykfM"
                     /locus_tag="ACHVB7_004551"
     CDS             <1..301
                     /gene="ykfM"
                     /locus_tag="ACHVB7_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFR1916310.1"
                     /translation="SFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLLIA
                     IVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(438..1223)
                     /locus_tag="ACHVB7_004552"
     CDS             complement(438..1223)
                     /locus_tag="ACHVB7_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916311.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          364 a          265 c          214 g          421 t
ORIGIN      
        1 gtcgttttcc tttttaaata ttcatgtaat tttacttacg ataataaaat tattcttaat
       61 caaaaaaaca aaggtagtca atttcgccac agacataaca atacagctaa ctcttatatt
      121 tttactaata gctatcgtaa tagcaccttt aatagctcct tttgtcacag gatatgtcaa
      181 tgctaattat cacccctgcg gtaataatac gggaattttc ccaggagcaa tctatattaa
      241 aaatggcatg aaatgtaata atgaatatat ttctcgcaag gaagattcag cagttaaata
      301 attaaacatt ctaaaacaaa cagtttttaa ctccggattc catgtccttt cttgcactgt
      361 ctttgtcaag aaaaatttct tatgcaaaga attttgtatt atttccgcca gaaattcctg
      421 gcggcagata taatttatta ttgatagcgg ttaaccaaat cttttaccat cgctttatag
      481 gtcttttgct taagttgcat gatttgctgc tctgtcatat cagtcgtact ggcttcattc
      541 ttgtcgggca atggtggaag cactttatat tcataattca ccggcgacca ggcagcccac
      601 tcacctgtag ctacatccac cagcgtgaat cgtaccagat agcgcagtga catcgtgtca
      661 gtcaattttt gatttttata atctgaccaa actattgatt tcaatccaga gtcatatttt
      721 cctgtctgca acgtatcctg gaaaacaata atcgctttct gatgcccttt tgcagccaca
      781 aaacgcagtg cctgcatcca gttcatattc tcagcactgg caacatcctc gttctcattt
      841 ttatctttgt ttttgttaca agtcagaggc ttttgcctgt ccgggatacc agagaatgtg
      901 gaaacagtat aatattttcg catctcctcc tgcataatgg tttccggtgc gcggttgccg
      961 gattgaacaa gtattacagg agaattaagg ggaacgcgga actcatctcc atcaagcgca
     1021 gcttgaatat cctcttcgga tacggccaga gtggtttcat taccgaataa atctttgtct
     1081 gtcagttgct ggggagtgtt tttattaata ttactttgtg tgacttgagc ttttttcttc
     1141 ttttcatcaa tagaagattc agagacacat cctgcaagca gcgagaataa tacacatata
     1201 caacaaaccc ttcttaaatt cataatcgga cactccctcg cctgtcataa tcatggtaat
     1261 gttt
//
LOCUS       DAZPJR010000364         1240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPJR010000364 DAZPJR010000000
VERSION     DAZPJR010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.372"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(29..829)
                     /locus_tag="ACHVB7_004553"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="ACHVB7_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          392 a          259 c          232 g          357 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta ttctatgaag ttcattttat tttaaagatt gcgtgtaaca aatgtattta
      961 aaagatttca aaatgtttct tatctatttg atataattca gaattatgaa ttctaaatat
     1021 aaacatccat gaaaattata aatgtatatc aatatgttat gagttgaatg gaaagatagg
     1081 gagtatcaat gattgttgcg ccaaacggca ggcgtgatca cgcgcacatt aatgaaggta
     1141 taaaaaaagc cggcatcatg ccggcccaaa aaatataaac aagacgataa aaggctaata
     1201 acggggttat catgacacag aaatcaaaaa aggtataatt
//
LOCUS       DAZPJR010000365         1212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPJR010000365 DAZPJR010000000
VERSION     DAZPJR010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.373"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            230..>1212
                     /gene="gspA"
                     /locus_tag="ACHVB7_004554"
     CDS             230..>1212
                     /gene="gspA"
                     /locus_tag="ACHVB7_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR1916312.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          328 a          249 c          310 g          325 t
ORIGIN      
        1 gcatctttat tatgattcgc cagatggaag gggaaacgta gtgtgggcac gatgtatgtt
       61 ctaataaaat agattgggtg ggttgtatat tgacgttagt ataaatagta ttaatgccgc
      121 ttgcatgaat caagcaaaat ttatttatat tagtaatatg tatttaatta ataaatacat
      181 acattatttt tatgattaag gataatcaat aattaaaaca ggaagttcta tgtctacgag
      241 aagagaagtt attctttcct ggttgcgtga gaaacgtcag acctggcgtc tgtgctattt
      301 gttgggtgag gctggaagtg gaaaaacctg gctggcgcag caactgcaaa aagataaaca
      361 tcgccgtgtg attactttaa gcctcgttgt ttcctggcaa ggtaaggccg catggatcgt
      421 taccgacgat aacgcggctg aacagggctg ccgtgacagc gcctggacgc gagatgagat
      481 ggcggggcaa ttactgcatg cgcttcatcg aactgatagt cgctgccctc ttattattat
      541 tgaaaatgct cacctgaatc accgccgcat acttgatgat ctccaacgcg cgatatctct
      601 tattcctgac ggtcaatttt tgctgatcgg cagacccgat cgaaaagtcg aacgtgattt
      661 taaaaaacag gacattgaac ttgtctcgac aggacgtctg acggagcacg aacttaaggc
      721 aagcatcctt gaaggtcaga atattgacca acccgatctc cttttaacct ccagagttct
      781 gaaacggata gctttattat gtcggggcga tcgcagaaag ctggcgcttg ccggcgaaac
      841 aattcgctta ttacagcaag ccgagcagac cagcgttttc actgcaaaac aatggcgaat
      901 gatttaccgc atattgggtg ataaccgccc ccggaaaatg cagcttgccg tggtaatgag
      961 tggaaccatt atcgctatta cctgtggttg gctactgctg tcctctttca cagctccgtt
     1021 ccccgttcct gcgtggctgg tacccgtaac gccagtagtt aaacaagata tgactaagga
     1081 tattgctcat gtggtgatgc gcgatagtga ggcgctaagc gtgttatacg gcgtatgggg
     1141 gtacgaggtc ccagctgatt cggcctggtg cgatcaggca gtaagagcgg ggctggcatg
     1201 taaaagtggg aa
//
LOCUS       DAZPJR010000366         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPJR010000366 DAZPJR010000000
VERSION     DAZPJR010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.374"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..680)
                     /locus_tag="ACHVB7_004555"
     CDS             complement(<1..680)
                     /locus_tag="ACHVB7_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1916313.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="ACHVB7_004556"
     CDS             complement(996..>1211)
                     /locus_tag="ACHVB7_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR1916314.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DAZPJR010000367         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPJR010000367 DAZPJR010000000
VERSION     DAZPJR010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.375"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            54..1211
                     /locus_tag="ACHVB7_004557"
                     /pseudo
     CDS             54..1211
                     /locus_tag="ACHVB7_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          321 a          305 c          273 g          312 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca g
//
LOCUS       DAZPJR010000368         1198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPJR010000368 DAZPJR010000000
VERSION     DAZPJR010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.376"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            223..1038
                     /gene="yahL"
                     /locus_tag="ACHVB7_004558"
     CDS             223..1038
                     /gene="yahL"
                     /locus_tag="ACHVB7_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR1916315.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          364 a          230 c          233 g          371 t
ORIGIN      
        1 tggtttaaca tattaacttc gctctccact taacttttta gttaaggaga gcgaaataat
       61 atcaaagtag cagtaaaacc tataacgtaa attcaaattg ttaaattaac tccctccagt
      121 acacaatact tctcacgtta gttatgagcg atttctgata gtgcctggtt taatcagcgc
      181 tttattatct gcgacgttta tttttattta agagagatcg cgatgatatc attaaaagct
      241 ccgcacaata atttgatgcc atatacgcaa caaagcatac ttaatacggt taaaaacaat
      301 cagttaccag aggatatcaa aagctcccta gtttcctgtg tcgatatatt caaggttttg
      361 attaaacaat attatgatta tccttatgat tgtcgtgatg atttagtcga tgacgacaaa
      421 ctcattcatc tcatggctgc cgtacgagac tgtgagtggt cagacgataa cgcactcacc
      481 ataaatgtgc agtttaatga tttcccagga ttttatgact ggatggatta ccctgatcac
      541 ccggttaagt ttgtttttcg catacttgaa aatcaaaaag ggacagtttg ggtatatgac
      601 caggatgatg cgtttcttga tattaaagca aatgtgcagg ctggacgctt taccgggctt
      661 aagaaattag tgcagtttat caattcagtg cgaacagatt gcaaatgtat tcttcttgaa
      721 caccatatgc ctttgctgag acttttccct aaagggaaag agtgtatgca tgtggaaaaa
      781 tggcttcgcg aaatgtcgtc tattccggag acagacgcgc ccattaagca ggcgcttgcg
      841 catggattat tattacactt aaaaaatatc taccctgttt ttcctgaatc actggtgatg
      901 ttattgctct ctgttctgga cgtaaaaaca tacagagatg atgcacgatt aaatgaatgg
      961 atatctaata gagtgcagga attaggtgac agatattatc ctgtaaataa gcacgttaaa
     1021 atccgctaca ctctttagtt cagaaagggt tccacctgca agttgttatt ccaccagaga
     1081 gtgaatcgca aagcaggtaa tcatttcatc tgtatatccg tatgccaaat ggcattccac
     1141 cgccttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagc
//
LOCUS       DAZPJR010000369         1191 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPJR010000369 DAZPJR010000000
VERSION     DAZPJR010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.377"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..1191
                     /gene="rhsB"
                     /locus_tag="ACHVB7_004559"
                     /pseudo
     CDS             1..1191
                     /gene="rhsB"
                     /locus_tag="ACHVB7_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
BASE COUNT          295 a          343 c          364 g          189 t
ORIGIN      
        1 ggtggtgtat gaccgtagtg gcaaacaggt gcgcagcttt acttacgatg ataaataccg
       61 gggccggatg gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag
      121 cgacgggcgg gtgacagaac agcttaaccc ggcaggctta agctacacgt atcagtatga
      181 gaaggaccgc atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgtaggg
      241 tgaagccggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag
      301 tcagtttgac gccgtgggca ggctcaggac acagacggat gccgcaggcc ggacaacaga
      361 atacagcccg gatgtggtga cgggcctcat cacgcgcatc accacgccgg atggcagggc
      421 atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct
      481 ggaaatgcgc cgcaaatatg atgaatccgg ccgtctgatt caggaaaccg cccctgacgg
      541 cgatatcacc cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga
      601 tgccaccggc agccggaaaa ccatgacatg gagccgttac ggtcagttgc tgaccgtcac
      661 tgactgttcc ggttatgtaa cccgttatga ccatgaccgt tttggtcagg tgacggcggt
      721 gcaccgcgag gaagggctga gccagtaccg cgcatacgac agccgtggac agttaattgc
      781 ggtgaaagac acgcagggcc atgaaatgcg gtatgaatac aacgccgccg gtgacctgac
      841 cgccgtcatt gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc
      901 catctgtacc acgcagggcg ggctgacgcg cagtatggaa tacgatgctg ccggacgggt
      961 catccgtctg accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgaccg
     1021 gctgatacag gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg
     1081 caaacttatc cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga
     1141 ccgcctcacg caccgcaccg tgaagggtga aaccgcagag cggtggcggt a
//
LOCUS       DAZPJR010000370         1190 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPJR010000370 DAZPJR010000000
VERSION     DAZPJR010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.378"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>1190
                     /locus_tag="ACHVB7_004560"
     CDS             <1..>1190
                     /locus_tag="ACHVB7_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1916316.1"
                     /translation="AEQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVS
                     RRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAP
                     CCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERG
                     IAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEAL
                     HGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDR
                     KGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALT
                     EEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELA
                     KAFA"
BASE COUNT          265 a          339 c          375 g          211 t
ORIGIN      
        1 gccgaacagg aagcgctgat ccacgccctg caggaaaagc ttagcaaccg ggagcgcgaa
       61 atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg cagtcgttcc
      121 gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct tcagaaagag
      181 agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc
      241 cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg
      301 gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga taccgccgaa
      361 cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga aaaacatgcc
      421 tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat cgagcggggt
      481 atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga gcacaccccg
      541 ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg ttcactgctg
      601 tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc gcttcatggc
      661 tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt gctgttgcca
      721 ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga ccgtaacgcc
      781 gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa aggcatccat
      841 ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata cgccgggttc
      901 aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca cgcccgccgt
      961 aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc gctgaaacgg
     1021 atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga acagcgcctt
     1081 gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg gctgcgtgaa
     1141 aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAZPJR010000371         1181 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPJR010000371 DAZPJR010000000
VERSION     DAZPJR010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.379"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..1000
                     /gene="htrE"
                     /locus_tag="ACHVB7_004561"
     CDS             <1..1000
                     /gene="htrE"
                     /locus_tag="ACHVB7_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR1916317.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1017..1121
                     /locus_tag="ACHVB7_004562"
                     /pseudo
     CDS             1017..1121
                     /locus_tag="ACHVB7_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          358 a          236 c          276 g          311 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggaa cgtcaaatga
     1021 taaaaataac gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat
     1081 ttgcaacgga tattaatgta gagtttacag ccactgtcaa agtagactgg ccccctgaat
     1141 ctccagacaa ccagtatcac ttaaataagt gatagtctta a
//
LOCUS       DAZPJR010000372         1176 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPJR010000372 DAZPJR010000000
VERSION     DAZPJR010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.380"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(354..977)
                     /locus_tag="ACHVB7_004563"
     CDS             complement(354..977)
                     /locus_tag="ACHVB7_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR1916318.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          314 a          246 c          246 g          370 t
ORIGIN      
        1 ctttctgcat ggtaagtagt gaggacgaaa agaagaatat gataaatatt tattcgtggt
       61 aatcagagag agtcgcgaca tttgtgatgt tattcttaca actgtaagaa gcgccggtga
      121 tttacaagta agagtatgat tacgcttttt aaagcgtacc ataaagcata acataataaa
      181 tgatttgtga tgacactgga ttattgcggt ttactttgag gaaaataaaa tgcgtgttta
      241 tagaaccgcg agcgccaaat aattttatga ccacaacctg tcgtattgtg gtcataaaaa
      301 attgtcggct ggagggagtt aaatccttta cctcaatata ctgcctgtca gcattagttc
      361 acaaacagtg cttcgctatc gacgatgatt ccatcttccg cacctttaag cgccaggttt
      421 gcctcgtagt attgcttttt atcaagcagt ttctgcgcat cagcaagtgc attacgagtc
      481 tgtttaagcg gcatcagata ttggttttcc attacgccaa caccggccag gcgaagctct
      541 tccattgcgc cctttttatc ccctttcgcc attttttcat tggcaatttt tatcgcggct
      601 tctttttcgg gtgttgctac ataactctca gaaattccga cagatgcatt aatgacaata
      661 tattggtcat cattgagatt ggtcttttta cctggcttag cgaactttgc ccattcggta
      721 ctgtcatctg acagcaaagc ggaagcttca ttggtcagtt cttttgcctt ttccggatcg
      781 ccatgaaaca gagccaggcg cgctacctgc acgtcacgca tcgcatataa accttgttca
      841 gagatacgct ctgctgctgc cgttttctgg gtttgctgag tttgctgttg tgccgctacc
      901 ggtgcattat cagcagccca tacagttgaa gatgccagaa ttgctgtgac catcgtagcc
      961 ataattaaac gtttcatact tacctcattc tttaacagtt ttagaaaacg ccgccatcat
     1021 attgggtgag catgaaatga gaaatcagag agcaacggtc attttgaaag ggttatctta
     1081 caattgtagt tattatcgca aagcggcatt tagtcttaca gttgtaggaa tatgcttcct
     1141 gtcgcagaga actattctta tataccacaa agagat
//
LOCUS       DAZPJR010000373         1169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPJR010000373 DAZPJR010000000
VERSION     DAZPJR010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.381"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..485)
                     /gene="umuC"
                     /locus_tag="ACHVB7_004564"
     CDS             complement(<1..485)
                     /gene="umuC"
                     /locus_tag="ACHVB7_004564"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1916319.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQT
                     GG"
     gene            complement(485..904)
                     /locus_tag="ACHVB7_004565"
     CDS             complement(485..904)
                     /locus_tag="ACHVB7_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1916320.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          335 a          301 c          241 g          292 t
ORIGIN      
        1 ccccccgtct gccgctgcca tttttttgcc gcatgattgg caagcttagc cagcgttttg
       61 gtctgggcga tccccatacc aacagtaaga tgggtacgtt gtagcaccgt tgcgcgaatt
      121 tctctgccaa aatcagtcag atcgcgacaa ttacgcacac ctgtcagatc gcagaatgcc
      181 tcatcaatac tgtaaatctc gacgcggggc gatagttctt ccagcgtcga catcacccga
      241 ttgctcatgt ctgcgtaaag ctcataattg ctgctaaagc aaaccacacc acagcgacga
      301 aacagatctt tttgtttgaa ccagggatcg cccattttaa cacccagcgc ctttgcctca
      361 gcgtttcggg cgataacgca accgtcatta ttcgatagca caaccaccgg tttaccccat
      421 aaatcagggc gaaacaccgt ctcacagctg gcataaaacg cgtttacatc acagagagca
      481 aacatcagcg catcgcctta acgacgtgga tcaccacacc aaagacatcc agcgtatctt
      541 cgctactgat ggtaatgggc gagtacgcac tgttcatggg aataagctgt accgtcgggc
      601 gtagttgcaa ttttttcacc gtaaactcgc cgtcaacagc agcgatgaca atatcaccat
      661 ggctggcggt aatagcgcta tcgacaatca gtaaatcacc gtcactaatt ccaccatcaa
      721 tcatagaatc accacttgct ttgacgaagt aagtcgcgct gggatgctgg atcaacagtt
      781 gattcagatc gatgcgctgt tcgacgtaat ctgctgccgg tgaaggaaag ccacactgaa
      841 caagatcgct aaatagcgga aaagtcacaa tttcgcggag atccgcaggc ttgataaaca
      901 acataataat ctgcctgaag ttatactgtt tttatataca gtaatctgtt cttgccagca
      961 gatcaatact gattcaggct atcaatattt gtcgctgcat agactgctga tttttcgttc
     1021 tcttatcctg tgctcacgtg gccttctggc gacgacgctc atccagcaga aatgaaaaat
     1081 atcacccggc aaaaaaatag aatagaagca ttcgccataa tgacattaaa cattgtttga
     1141 tatttatcat attaatagaa ataaagaca
//
LOCUS       DAZPJR010000374         1162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPJR010000374 DAZPJR010000000
VERSION     DAZPJR010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.382"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHVB7_004566"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHVB7_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR1916321.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1162)
                     /locus_tag="ACHVB7_004567"
     CDS             complement(510..>1162)
                     /locus_tag="ACHVB7_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR1916322.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          352 a          277 c          276 g          257 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cgggggtcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa ta
//
LOCUS       DAZPJR010000375         1142 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPJR010000375 DAZPJR010000000
VERSION     DAZPJR010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.383"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHVB7_004568"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHVB7_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1142
                     /locus_tag="ACHVB7_004569"
     CDS             572..>1142
                     /locus_tag="ACHVB7_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR1916323.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLICLCY
                     SQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPMADFSS
                     VSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPRLNKLV
                     TEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          319 a          255 c          271 g          297 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctaat ctgtctgtgt tactctcaga tatccctggc
      721 gggtattgtc cgttccgata ttgcctatca gatttatcgt gatttcgccg aaaacaaagg
      781 gctttttgta cctggtgcca atgatattcc ggtatatgat aaggacggaa aacttgtggg
      841 aagactgggt aaagccccaa tggccgattt cagcagtgtg agctcaaatg gcgttgctac
      901 gcttgtatcg cctcagtata tcgtcagcgt aaagcataac ggaggatatc ggagtgtgag
      961 ctttggtaat gggaaaaata catattccct tgttgaccgt aataaccacc cttctattga
     1021 cttccatgct ccacgtctga ataaactggt tacagaagtt attccctcag cggtaacatc
     1081 agaaggaacc aaagccaatg cttataaata cactgaacgt tacaccgctt tttatcgggt
     1141 gg
//
LOCUS       DAZPJR010000376         1132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPJR010000376 DAZPJR010000000
VERSION     DAZPJR010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.384"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..317)
                     /locus_tag="ACHVB7_004570"
     CDS             complement(<1..317)
                     /locus_tag="ACHVB7_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR1916324.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRP"
     gene            complement(334..>1132)
                     /locus_tag="ACHVB7_004571"
     CDS             complement(334..>1132)
                     /locus_tag="ACHVB7_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1916325.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          300 a          258 c          226 g          348 t
ORIGIN      
        1 ggtcttatga tctttccctt acttaataaa tagcatgtat aggattcgga aagtccagca
       61 tctattttgt acctcattat attagataag gatgtgagaa attccttgtt atcattcgca
      121 gccatatatg ccagtccgag ttcagcctct gcggccttac aggcaaaata gcttagtgaa
      181 cacgcttttc gtccctcttt agtactggct gatattagtg cccataatat tgctttgtct
      241 gccaaatcta actctctctg aaattgtctt gcagtaactg cactcgcaga aatagagaaa
      301 aaaatacaat tgttaataac tttgccatat tttttagtcc gtaactgtta atgtgctatc
      361 tggctctgaa aatattaact catttaattt ttgcatgtca ttagatgaac cagttataca
      421 accctcggaa cagaatcgag gtcctaaaga cggaccgaaa gcgtttaaac aactttgtgt
      481 taaaaatcca tcacgattat atgtttctgt atttgctgac ggtactaaac gtataccatt
      541 aggttttcta ttggttgctc caggaccatt aacattccag ctccataccc cttgaggtat
      601 tggacctcta ttttgtaagt gtgtacaacc aggattattt ttacattgca tattattacc
      661 attattacca gaggcgacag gaatagtaag cacatcataa gagttatcat cattttgcgt
      721 atctgaaacg catgaaagca tacttaaagt tatgctgtat aaacaggtcg ctaatcccaa
      781 cgggtcaata tcagttatcg gattcaacgg atactgataa aaattccatc cccccttcag
      841 tccaatcgga tcctgagtga tatatcgccc ctgcagcggg tcataatagc ggtggcggtt
      901 gtaatacagg ccggactcct catcatactg ctgccccggc aggcggataa gctgctgcag
      961 ctgatgcggg ttctcttcat tcagcaggtt gccccactca tcgtattctg cgcaccattc
     1021 tgttgcccct tccgtgctga taagcaccag cggcaggccg cgatggtcgc agtggtacag
     1081 gtggattttt cgcgccggcg tgtacaccgg gtccatctgg ttttgcatct gc
//
LOCUS       DAZPJR010000377         1117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPJR010000377 DAZPJR010000000
VERSION     DAZPJR010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.385"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHVB7_004572"
     CDS             84..1115
                     /locus_tag="ACHVB7_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR1916326.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAZPJR010000378         1091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPJR010000378 DAZPJR010000000
VERSION     DAZPJR010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.386"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHVB7_004573"
     CDS             complement(<1..228)
                     /locus_tag="ACHVB7_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR1916327.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHVB7_004574"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHVB7_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          357 a          176 c          204 g          354 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag a
//
LOCUS       DAZPJR010000379         1089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPJR010000379 DAZPJR010000000
VERSION     DAZPJR010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.387"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..229
                     /locus_tag="ACHVB7_004575"
                     /pseudo
     CDS             1..229
                     /locus_tag="ACHVB7_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            454..945
                     /locus_tag="ACHVB7_004576"
     CDS             454..945
                     /locus_tag="ACHVB7_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1916328.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          432 a          169 c          163 g          325 t
ORIGIN      
        1 atgatttaat gagtatggct gtaaatgaac tcataaaact gaccaaaata gagaataatg
       61 tagacctgaa taaattcact actatatgca cagacgttct atcccccctc gtcaccagac
      121 ataataaaga aaaaaaacaa acgacattct acccttctca aaaatcccct ttttaatttt
      181 cattgaaaaa atggcaattg atactatgaa tccaggtcat acacattaag gcatttatgt
      241 aatgtcgtaa ttaagataac taataaggtg aatattagta aaacaactaa tgatgttatt
      301 agtttgtagt gaacagtact tttaccaata atgaaaaata taccataagc aatgcaagct
      361 taaaactaat aatttaaaaa agataaatgt aaaaatatta atgagtcaat caataataat
      421 ttagcatcac gagacacatc acagaggaat attatgagca cagaaacaat tgaaatattc
      481 aataatagtg atgaatgggc aaatcaacta aaacacgcat tatcgaaagg agaaaatctg
      541 gcattactac atggtttaac tcctgatatc cttgatagaa tatatgcata tgcattcgac
      601 taccatgaaa agggtaatat aacggacgca gaaatttatt ataaattttt gtgcatttat
      661 gcgttcgaaa atcatgagta tctaaaagat tttgcatcag tatgtcagcc aaaaaagaaa
      721 tatcaacaag catatgacct ttacaaacta agttacaatt actccccgta tgatgactat
      781 tcagttattt atcgtatggg tcaatgtcag attggggcta aaaatatcga taacgcaatg
      841 caatgtttct atcacattat taacaattgt gaggatgata gtgttaagag taaagcgcag
      901 gcatatattg aactcttaaa cgataattca gaagataatg gctaagcatt acaatcattt
      961 ggttaaattt gctggttata gtgcagttaa ctactataac cagcacctct tatgttttct
     1021 taaatcagca tcttaaaaca gttaagagca tactataaat cattattttt caatgcgtta
     1081 gagaacaat
//
LOCUS       DAZPJR010000380         1088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPJR010000380 DAZPJR010000000
VERSION     DAZPJR010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.388"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            212..>1088
                     /gene="nadA"
                     /locus_tag="ACHVB7_004577"
     CDS             212..>1088
                     /gene="nadA"
                     /locus_tag="ACHVB7_004577"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1916329.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          294 a          259 c          265 g          270 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgca
//
LOCUS       DAZPJR010000381         1085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPJR010000381 DAZPJR010000000
VERSION     DAZPJR010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.389"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHVB7_004578"
     CDS             complement(<1..643)
                     /locus_tag="ACHVB7_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1916330.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHVB7_004579"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHVB7_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1916331.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAZPJR010000382         1059 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPJR010000382 DAZPJR010000000
VERSION     DAZPJR010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.390"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..>1059)
                     /locus_tag="ACHVB7_004580"
     CDS             complement(<1..>1059)
                     /locus_tag="ACHVB7_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1916332.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLV
                     LTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTH
                     DPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPE
                     SCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKE
                     HADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQR
                     QVTSVTYPDGLRSSREYDE"
BASE COUNT          164 a          409 c          294 g          192 t
ORIGIN      
        1 ctcatcatac tcccggctgc tgcgaagccc gtccgggtac gtcaccgacg tcacctgccg
       61 ctggctgtta tagccatacc gtaccgtcct gccgtccggc agtatcactg aagtcagctt
      121 tccagacgcc atatgcagtc ggtactccgt ccgccgtccc gccgcatccg tctgcgcctt
      181 cagcctcccc gcctcatcat actcgctgcg ggtgatgctc ccgtcggcat gctccttctt
      241 caccacccgc ttcaggccac cctcgccttc cgtgtacagc acctcccgcc ggttcaggct
      301 gtccgtgatg attacccggc tctctccgta ctcaaagcgg tagtccagcc cctccgggtt
      361 gacctgctcc gtcacccgac cggtatcatc ataccggtag cagctctccg gcctccccgc
      421 ataatggtgc gccaccatcc gcccggcgtg ctccgcatca taagtaaacc cgcgcacctg
      481 catcccgctg cggtcataca ccgcccgcag ttcaccaccg gccgtgtacg tgtagcgcgc
      541 cagcggcgca gtcggctgtt catccgggta tgccgggtcg tgtgtcagcc ataccgcctc
      601 cagccggata ccgttatcgg caccgtactc tgtaccggcg ggcagcgtgt cggggaagga
      661 aggagctgag agagggcagg gactgtcagg ggaagataac gaagcggtgt gcggtttacg
      721 ggcctcttcc gcccgctgcg cctgtgtggt cagcaccagg tgaaaacggc ggcccgcgcc
      781 gtccatcacc cccgtcaccg cgcccgccac gtcaccctcc gctgcccggt gaaaggccag
      841 cgtccgcccg aagccgtcca ccacgcccgt cagcacccgg tatgcgggcg gctccggcgg
      901 cagcacctcg tccgcccccg gcacccgctc cggccagctg agtatccacc acggcccctg
      961 aaggctgttt gtcgccaggt acacatgcgg actcaggcga acatcttccg gcagcacctg
     1021 ccagagcgcg cttagcggct gcgaactgtg ctgcgccgc
//
LOCUS       DAZPJR010000383         1055 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPJR010000383 DAZPJR010000000
VERSION     DAZPJR010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.391"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            44..1055
                     /locus_tag="ACHVB7_004581"
                     /pseudo
     CDS             44..1055
                     /locus_tag="ACHVB7_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          413 a          183 c          184 g          275 t
ORIGIN      
        1 gtaatatata tttgtaacaa atcaatcaaa atggaataaa atcatgctac catctatttc
       61 aatcaacaat accagcgcaa cttacccaga atccattaat gaaaataaca atgatgaagt
      121 taatggatta gtacaagagc tcaaaaacct ttttaatggt aaggaaggaa taagcacctg
      181 tattaaacat ctacttgagc ttataaaaaa cgccatacga gtaaacgacg atccttaaga
      241 tttaatatta ataattcctc agttacttat attgatattg gctccaatga tacagaccat
      301 attactattg gtatcgacaa ccaagaacca atagaattac ctgcgaacta taaagacaaa
      361 gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga tatcaataac
      421 aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg ttttatcttc
      481 gataagatat cacacaaact cagacatacg gtaacagaat ttgatgagag cgggaatagc
      541 gaaccaacgg acttatttac ctggtacggt aaagataaaa aaggcaactc tctcgctatt
      601 gtaattaaaa ataaaaacgg aaatgattac ttatctctcg gttactacga tcaggacgac
      661 taccacattc aaagaggaat tcgtattaat ggtgatagtc tcacccaata ttgtagtgaa
      721 aaagccagga atgcttcagc aatgcttcag cgtggtttga aagcagtaaa gctatcatgg
      781 cagaatcatt tgcaacaggt tccgatcatc aggttgtaaa cgagctcaac ggggaaagac
      841 tgagagaacc taacgaagtt tttaaacgtt taggtcgagc aataagatat aattttcaag
      901 tggacgatgc aaaatttaga cgcgacaatg taaaagaaat aatttctaat ttattcgcta
      961 acaaagttga cgttgaccat cctgaaaata aatacaaaga ctttaaggat ctcgaagata
     1021 acgttgaaaa aagacttcaa aatcgccagg cgaaa
//
LOCUS       DAZPJR010000384         1045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPJR010000384 DAZPJR010000000
VERSION     DAZPJR010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.392"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHVB7_004582"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHVB7_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHVB7_004583"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHVB7_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="ACHVB7_004584"
     CDS             728..985
                     /locus_tag="ACHVB7_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR1916333.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          179 c          198 g          311 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccc
//
LOCUS       DAZPJR010000385         1042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPJR010000385 DAZPJR010000000
VERSION     DAZPJR010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.393"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            90..>1042
                     /locus_tag="ACHVB7_004585"
     CDS             90..>1042
                     /locus_tag="ACHVB7_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR1916334.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKVESYNSALQAFSIFRSS"
BASE COUNT          385 a          156 c          194 g          307 t
ORIGIN      
        1 agcaattgtc tcaaatgagc gattttttat agtgacaaac aataataaaa ttaaaaatag
       61 ccatgtagac attaactgag gaatagacaa tgttagttag taaaagcaac gaatttaaca
      121 ctagctcatt tatagatagt ggaaattgta atgaaagaaa atcttctgaa tccatggagc
      181 tactagctta tagtattata aatttaattt gtaaggaagc tgcctcaggg acgtatcgcg
      241 gtgctcttga aactttacaa aaaatgatgt ctgaatgtat atatcatgaa ggcaacgcct
      301 ttgtcattat gggatctgga gagcaattaa aacgtattaa atatgatgtt gatgaaaata
      361 acttaaaagt attcaacgta tactttgata ataatgaaga gttagttact gatggtgagc
      421 ctgacgtagt atgtttaagc aagcaggtct gggaagatct tctcattaaa ctaaagctgg
      481 agaacaaaga aaatgcggtt tctgaaactg acctgtcatc gaataaaaat aatgttgatc
      541 agtttattga atgcgctaag agaaatgaac agaccctatt cgataatata agaaaaagtg
      601 attttcatgt tgattcattt aagccaggta gtacacgtag tgttatttta gaaacacaac
      661 caaatgtctg tatggaacca cataatttat atgacaacca aatagataag gtttcacctg
      721 taactaaaaa ctctcagcaa gttaaaggcc actatggaga taaatttaaa gaaatgcaga
      781 tgttccttaa ccagatgagc aatgcacttc aacagactcc atctttgtca gagtcaaagg
      841 aacacaccat agatattcaa aaaataacgg atgcctttgt gaaagaattc cggggggtat
      901 taattgataa aaatgggaat tgttcagaac gtttattcaa tttttatgag tgctgctata
      961 agtttttacc aagagcgcag cctcaagata aagtcgaaag ctataattca gcactacaag
     1021 ctttttccat ctttcgttca tc
//
LOCUS       DAZPJR010000386         1026 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPJR010000386 DAZPJR010000000
VERSION     DAZPJR010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.394"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..>1026)
                     /locus_tag="ACHVB7_004586"
     CDS             complement(<1..>1026)
                     /locus_tag="ACHVB7_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFR1916335.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     PPAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASR
                     KQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTA
                     PLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRV
                     TEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEY
                     DEAGRLKAQTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGL
                     RSSRKYDR"
BASE COUNT          172 a          381 c          283 g          190 t
ORIGIN      
        1 acggtcatat ttccggctgc tgcgtaaacc atccggatat gtcactgacg tcagttgcaa
       61 ctggttgttg taaccatacc gtaccgtcct gccgtccggc agtaccactg aagtcagctt
      121 tccagacgcc atatgcagcc ggtactccgt ccgccgcccc gccgcatccg tctgcgcctt
      181 cagcctcccc gcctcatcat actcgctgcg ggtgatgctc ccgtcggcat gctccttctt
      241 caccacccgc ttcaggccac cctcgccttc cgtgtacagc acctcccgcc ggttcaggct
      301 gtccgtgatg attacccggc tctctccgta ctcaaagcgg tagtccagcc cctccgggtt
      361 gacctgctcc gtcacccgac cggtatcatc ataccggtag cggctctccg gcctccccgc
      421 ataatggtgc gccaccatcc gcccggcgtg ctccgcatca taagtaaacc cgcgaacctg
      481 cgtcccgctg cggtcataca ccgcccgcag ttcaccaccg gccgtgtacg tgtagcgcgc
      541 cagcggcgca gtcggctgtt catccgggta tgccgggtcg tgtgtcagcc ataccgcctc
      601 cagccggata ccgttatcgg caccgtactc tgtaccggcg ggcagcgtgt cggggaaaac
      661 ctgagaagag gaaacagaac ggggactggc cggggaagat aaggaagagg cgcgctgttt
      721 acgggacgct tccgcccgct gcgcctgcgt gctcagcgcc atgtgaaaac ggcggcccgc
      781 gccgtccgtc acccccgtca ccgcgcccgc cacgtcaccc tccgccgccc ggtggaaagt
      841 cagtgagcgc ccgaagccat ccaccacgac cgtcagtacc cggtatgcgg gcggctccgg
      901 ccagctgagt atccaccacg gcccctgaag gctgtttgtc gccaggtaca catgcggact
      961 caggcgaaca tcttccggca gcacctgcca gagcgcgctt agcggctgcg aactgtgctg
     1021 cgccgc
//
LOCUS       DAZPJR010000387         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPJR010000387 DAZPJR010000000
VERSION     DAZPJR010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.395"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHVB7_004587"
     CDS             complement(77..865)
                     /locus_tag="ACHVB7_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1916336.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHVB7_004588"
     CDS             complement(905..>1023)
                     /locus_tag="ACHVB7_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1916337.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZPJR010000388         1002 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPJR010000388 DAZPJR010000000
VERSION     DAZPJR010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.396"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>1002
                     /locus_tag="ACHVB7_004589"
     CDS             <1..>1002
                     /locus_tag="ACHVB7_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFR1916338.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          221 c          254 g          234 t
ORIGIN      
        1 gcagtggtaa cctgatcgaa ggtctcactg tgtactttgc cttaaaaagc ggctctgcca
       61 cattaacgtc attaacagcg gtgaccgatc aaaacggaat cgcgacaaca agcgtgaaag
      121 gagcgatgac aggtagcgtc acggtaagcg cagtcacgac cgctggtgga atgcaaacag
      181 tagatataac gctggtggca ggcccagcag acgcctcaaa gtcagtcctt aagaacaatc
      241 ggtcatcatt gaaaggagac tttaccgata gtgctgagct acatcttgtt ctgcacgata
      301 tatcaggcaa tccgatcaaa gtttctgaag ggctggaatt tgtacagtca ggtaccaacg
      361 tgccctatgt gcaagttagt gcaattgact acagtaaaaa tttctcaggc gagtacaaag
      421 ccactgttac aggaggcgga gagggtatca caacgctgat ccctgtattg aatggtgttc
      481 atcaagctgg tctgagtacc acaatacaat tcactcgcgc agaagacaaa ataatgagcg
      541 gtacagtatc agtcaatggt actgacctac cgacaactac attcccttcg caggggttca
      601 ccggggcgta ttatcagttg aataatgaca actttgcccc aggaaaaacg gcggctgatt
      661 atgagttttc aagctctgcc tcctgggtcg atgttgatgc taccggtaaa gtgacattta
      721 aaaatgtcgg cagcaattcg gaaaggatta cggcgacacc aaaatcagga ggccctagct
      781 atgtatacga aatccgtgtg aagagttggt gggtgaacgc cggcgaggct ttcatgatat
      841 acagccttgc tgaaaatttt tgcagcagca atggctacac gctccccaga gcaaactatt
      901 taaaccacag tagttcccga ggcatcgggt cactgtacag tgaatgggga gatatggggc
      961 attacacgac tgaagctggt tttcaatcaa atatgtattg gt
//
LOCUS       DAZPJR010000389         1000 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPJR010000389 DAZPJR010000000
VERSION     DAZPJR010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.397"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..928)
                     /locus_tag="ACHVB7_004590"
     CDS             complement(<1..928)
                     /locus_tag="ACHVB7_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1916339.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASK"
BASE COUNT          317 a          185 c          139 g          359 t
ORIGIN      
        1 tttttgatgc taatattttt gctcgtcttt tatctctctt gccagaaaga ttacaatcat
       61 tcaattcttt ttcattgctt tcaactaatt gatttaatag ttcaaaatca ccattaagta
      121 gaacattcca taaataacga gttttatgat aagctctatt ttgactcaat aatgtatgaa
      181 tttcttcttt cctgttacct ttgtataact gcatcacaga agagtagatt ctttcgtgcg
      241 caaatgactc atgtcgataa tgagtgatgg ttctacatga gttcattaag gaattttctc
      301 tatagttatg cttatacata taatatggaa ccgcatacat atcgcatgta attagcagtc
      361 gatagataaa atccagatct tctcctagtc gtaaatcttc atcaaaaaaa agattattct
      421 cttccagaag ttgttttctt ataaacaagc accctatatg gaatctaacc tttttataaa
      481 aaaagtcgaa aagtaaagat ccttcattaa actctgtatt tataggtgag taagtaacac
      541 catcttttat ataatggcca taaagcccac aataaatcgc atcccctccc gtctcatgtt
      601 gtctggtata catcttcagc agatagttag gatctatctc atcatcatca tccataaaac
      661 atataaactg tccttgcgcc ttgcggatac ctgtattcct ggcagacgaa acaccgccat
      721 tttctttatt gataataatt aatcgagagt gggttaaccc atactctctc aaaacgtttg
      781 atgtgttatc tttagaacca tcattaacaa taacaatttc aaaatttggg taagtttgtt
      841 tttctaattt cgttagagtt tcagtaatgt aatcagatga attatacgtc ggtattatta
      901 tggatactaa agggtaatca ttgctcatcg tgttctcttg cctgaactaa taaaatatga
      961 tagtactcat ccttaattat tattgtaact cagacatcca
//
LOCUS       DAZPJR010000390          971 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPJR010000390 DAZPJR010000000
VERSION     DAZPJR010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.398"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..133)
                     /locus_tag="ACHVB7_004591"
     CDS             complement(<1..133)
                     /locus_tag="ACHVB7_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1916340.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="ACHVB7_004592"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="ACHVB7_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          297 a          221 c          184 g          269 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gtagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggtgact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtgt tggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccacgcctta tatccctgcc
      601 ctgaaggacg gggttttacg gcgcaccgga taagcctgga gattatctac accagcatgg
      661 acatcattgt actatgcccc gtacatgccc catcacatcc tcaacatcaa agtattagag
      721 ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa aaaaatagaa
      781 ctaatatcaa gcggcgctat attgcgtatt aaataaaacc acctcaatta cattaataaa
      841 tctaaacaaa catcatgcaa ataatttcca tggttatttc taggctattt aaattaaata
      901 aataaattat gagtcaatca taaataacaa cctttaatat aatttaacta ccaaatttat
      961 attcgttcac a
//
LOCUS       DAZPJR010000391          969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPJR010000391 DAZPJR010000000
VERSION     DAZPJR010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.399"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..955
                     /locus_tag="ACHVB7_004593"
     CDS             <1..955
                     /locus_tag="ACHVB7_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1916341.1"
                     /translation="GDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSTDDKDV
                     IAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLD
                     IKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNPAHD
                     SSAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEM
                     MVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIR
                     HIAINVLTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          311 a          177 c          237 g          244 t
ORIGIN      
        1 tggtgatttt gaaaatggca ttcctgttca cgataccatt gccagagttg tatcctgtat
       61 cagtcctgca aaatttcacg agtgctttat taactggatg cgtgactgcc attctacaga
      121 tgataaagac gtcattgcaa ttgatggaaa aacgctccgg cactcttatg acaagagtcg
      181 ccgcagggga gcgattcatg tcattagtgc gttctcaaca atgcacagtc tggtcatcgg
      241 acagatcaag acggatgaga aatctaatga gattacagct atcccagaac ttcttaacat
      301 gctggatatt aaaggaaaaa tcatcacaac tgatgcgatg ggttgccaga aagatattgc
      361 agagaagata caaaaacagg gaggtgatta tttatttgcg gtaaaaggaa accaggggcg
      421 gctaaataaa gcctttgaga aaaaatttcc gctgacagaa ttaaataatc cagcgcatga
      481 cagttccgca atgagtgaaa agagtcacgg cagagaagaa atccgtcttc atattgtttg
      541 cgatgtccct gatgaactta ttgatttcac gtttgaatgg aaagggctga aaaaattatg
      601 cgtggcagtc tcctttcgct caataatagc agagcaaaag aaagagcccg aaatgatggt
      661 cagatattat atcagttctg ctgatttaac cgctgagaag tttgcgacag cgattcgaaa
      721 tcactggcac gtggagaata atctgcactg gcgtctggac gtggtaatga atgaagacga
      781 ctgcaaaata agaagaggaa atgcagcaga attattttca gggatacggc acattgctat
      841 taatgttttg acgaatgata aggtattcaa ggcagggtta agacgtaaga tgcgaaaagc
      901 agccatggac agaaactatc tcgcgtcagt ccttgcgggg agcggacttt cgtaatcttg
      961 ccctgagga
//
LOCUS       DAZPJR010000392          962 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPJR010000392 DAZPJR010000000
VERSION     DAZPJR010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.400"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..400
                     /locus_tag="ACHVB7_004594"
     CDS             <1..400
                     /locus_tag="ACHVB7_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR1916342.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            574..>962
                     /locus_tag="ACHVB7_004595"
     CDS             574..>962
                     /locus_tag="ACHVB7_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1916343.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHT"
BASE COUNT          328 a          177 c          190 g          267 t
ORIGIN      
        1 tgatgtgatt aatgccattg tcaaaccgtt ctatgaacgt cagatgagta agtcagataa
       61 tccaattgat aaagttgtaa ggtcaaaggt ggaacccaca gaccaagcat tgactggtga
      121 catgaactct ttagccgctg ttttatgggc tatggttcag ggcaaatcaa aagatgttcc
      181 acagttttca cctgaagcat taaataatct gaaaggtcga gtggaaacat tcacacaaga
      241 tttaaaggtt atcaaaccgt cagtaaatat tcagcctcag acaatcaata tcacaactca
      301 gacggtactt gatggaaagg ttattgatga aagaaccaca tcgcatatta atcgtatgca
      361 ggaagatacc ttgattagct cagcttatcc agaggaataa acaatgttag agctactaat
      421 atttattgcc acacatggca atctattaat ctttatctag tatgacgatt caactatgaa
      481 tagtcgaatg ctacgtgaaa tcttctgaac agataccaac atcaaaaatt agatttcacg
      541 tagctcacgc ttaacagaat aggaataaca acaatgtttg acacaaagca aattagatac
      601 tcaggtaaag atggtatcta ttttcacctt agagataatg tggaatcttt tcttgcctta
      661 acagctactg aacagatgga ctatgactca ccgatgatgg tgacaactca ggctatgcaa
      721 tcggggcaaa ctatcactga taatgttcag agagcaccta agaccatctc tattagcggt
      781 gttgtagtgg ttgattcgac gggttcagtt ctattaacca gacaggggca acttgtagaa
      841 acctttattt ctacggttga acaatggcga gaccagaagc aaatcatcac tattgtttgt
      901 agagatggaa tagctatcac tgatagtatc attactcagt ttaaagcgag taaagagcat
      961 ac
//
LOCUS       DAZPJR010000393          960 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPJR010000393 DAZPJR010000000
VERSION     DAZPJR010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.401"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..851
                     /locus_tag="ACHVB7_004596"
     CDS             <1..851
                     /locus_tag="ACHVB7_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1916344.1"
                     /translation="RTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLT
                     SATGPDGLEIRREYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQ
                     GRYITQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESIN
                     ENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKG
                     NKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTI
                     IRQGSN"
     gene            853..>960
                     /locus_tag="ACHVB7_004597"
     CDS             853..>960
                     /locus_tag="ACHVB7_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFR1916345.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAA"
BASE COUNT          316 a          171 c          232 g          241 t
ORIGIN      
        1 tcaggacaca gacggatgcc gcaggccgga caacagaata cagcccggat gtggtgacgg
       61 gtctcatcac gcgcatcacc acgccggatg gcagggcatc ggcgttttac tataaccacc
      121 acagccagtt aacgtcagcc accgggcctg acgggctgga aatacgccgg gaatatgatg
      181 aatggggcaa cctgctgaat gaagagaacc cgcatcagct gcagcagctt attcgtttgc
      241 cggggcagca gtatgatgag gagtccggcc tgtattataa ccgccaccgc tattatgacc
      301 cgctgcaggg gcggtatatc actcaggatc cgattgggct gaagggggga tggaacctgt
      361 atacatatcc gctgagcccg gtgaatagca tggatccatt aggattatat gaatttaaat
      421 caaaaaatat tgatgatatt ggaatatttg cattggcaat gtgtaatgga gaatcaatta
      481 acgagaataa agaatatggt ggactaatat gtaagaagca aggtgaatat ttccccatga
      541 atccgataag ttcaaatgat aatgatagtg tagacttgcg aaatataaaa tgccctgaag
      601 gttcagagag agtaggcgat tatcacactc acggttttta ctctgacgat aaaggaaata
      661 aagtaacaaa agaaaatgat gtttatgata gtctaaattt ttcaagcaaa gatttaacga
      721 attcttatat gaatggaatg ggaaaaaaag aatacagtag ttacttggga acaccaaata
      781 acacctatct aaaatataat cccaaagcta aagggaatgg agttacaatt atcaggcaag
      841 ggagtaatta aaatgaaggc aatacaatgt tgttatttcc ttgctttagt ctccagtcat
      901 ttaatggctg gagttatggg tgatgataat atatttttaa gtaattcgga ttcagctgct
//
LOCUS       DAZPJR010000394          959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPJR010000394 DAZPJR010000000
VERSION     DAZPJR010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.402"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>959
                     /locus_tag="ACHVB7_004598"
     CDS             <1..>959
                     /locus_tag="ACHVB7_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HFR1916346.1"
                     /translation="ILGYQANSNGKVIVEKGGEWLIKNNDSSIEFQIGNQGAGEATIR
                     EGGLITAENTIIGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKE
                     YSLVGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIIT
                     AGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGV
                     GNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLM
                     DATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASG"
BASE COUNT          272 a          173 c          266 g          248 t
ORIGIN      
        1 cattttagga tatcaagcga acagtaatgg taaggttatc gttgaaaagg gtggcgagtg
       61 gctaataaaa aataatgatt cctcaattga atttcaaatt ggtaatcaag gagctgggga
      121 ggcgactatt cgcgagggtg ggctgattac ggctgaaaat acgattatcg gtggcaatgc
      181 caccggtatc ggaaccctga atgtgcagga tcaagactct gtcatcacgg tacgcagact
      241 ctataatggg tatttcggta atggcacagt caatatttcc aataatggtc tgattaataa
      301 caaagaatat tcattggtgg gcgttcagga cggttcccac ggtgtcgtca acgtgaccga
      361 taaagggcat tggagtttcc tcggaacagg cgaagctttc cgctatatct atatcggtga
      421 tgctggtgac ggtgaactta atgtctcgcg tgaaggtaaa gtagattcgg gaattatcac
      481 tgcggggatg aaagaaacag gcacaggcaa cattactgtt aaggataaga actccgttat
      541 cactaatctc ggaactaatc ttggttatga cggccacggc gaaatgaata tcagtaatga
      601 ggggcttgtt gtcagcaacg gaggaagctc actcggttat ggagaaaacg gcgtcgggaa
      661 tgtcagcatc accacggggg ggatgtggga ggtcaataag aatgtctata ccaccattgg
      721 tgttgcgggc gtcggaaacc tcaatattag cgatggcggt aagtttgtat cgcaaaatat
      781 tacttttttg ggcgataaag caagcggtat cggcacactg aacctgatgg atgcgacatc
      841 atcgttcgat actgtgggta tcaatgtcgg taattttggt agcggtatcg taaatgtcag
      901 taatggtgcc acccttaatt caacgggcta tggatttatc ggaggaaatg cctccggta
//
LOCUS       DAZPJR010000395          949 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPJR010000395 DAZPJR010000000
VERSION     DAZPJR010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.403"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>949
                     /locus_tag="ACHVB7_004599"
     CDS             <1..>949
                     /locus_tag="ACHVB7_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916347.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGINTLMNEGFLTKKDLKDFANNSAGLISKAVNGMVKKGYTDAQIGS
                     RLEDLADDLLLTSKYWQRSAREVQASITQQKAAGQWLTET"
BASE COUNT          325 a          190 c          238 g          196 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaaaatac tagatgccat cggtaaagat
      601 gtaaaggaac gcgctaccaa aagctatgac gaggctgaat atgtagttga taagaaaaca
      661 gggaaaggca tatggaaggg cggtgattct ggtattaaca ctttaatgaa tgaaggattc
      721 ttaactaaaa aggatttgaa agactttgcc aataactctg ctggtttaat ttcaaaagct
      781 gtcaacggaa tggtgaaaaa gggctatacc gacgcacaga tcggtagtcg tctggaagat
      841 ttagcggacg acctcctctt aacttctaaa tattggcaaa gatcagccag agaggtgcag
      901 gcaagcatta cccagcaaaa agccgccgga caatggctaa cagaaacac
//
LOCUS       DAZPJR010000396          945 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPJR010000396 DAZPJR010000000
VERSION     DAZPJR010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.404"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..945
                     /locus_tag="ACHVB7_004600"
                     /pseudo
     CDS             1..945
                     /locus_tag="ACHVB7_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          234 a          233 c          286 g          192 t
ORIGIN      
        1 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
       61 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      121 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      181 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      241 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      301 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      361 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gatcatgcgc cagaatgcgc
      421 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      481 ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg
      541 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
      601 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      661 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      721 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      781 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      841 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      901 cagaggcgtt cgagcattat aacgaatggc atccgcatag tgcgc
//
LOCUS       DAZPJR010000397          936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPJR010000397 DAZPJR010000000
VERSION     DAZPJR010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.405"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            50..936
                     /gene="espX1"
                     /locus_tag="ACHVB7_004601"
                     /pseudo
     CDS             50..936
                     /gene="espX1"
                     /locus_tag="ACHVB7_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
BASE COUNT          365 a          156 c          130 g          285 t
ORIGIN      
        1 tcactcataa attaaattaa taaaaattca tcaacttcaa ggactgataa tgacaaatgg
       61 tatctcaact tcgccacatt gtctttataa atcaaatata gtagatgatg taataataaa
      121 taaaacaaga caaaatgagt tagtaaaagt attttgtgag tataaaacag agttcttaat
      181 tttgtttgat gactttttta gaagccataa cttgcctaaa ccatctccag tccttcatca
      241 tttttttcaa tatactcatc tgagagatgc atatttttat agatgtaaac tcatagaaca
      301 tacagttcaa ttttattttt taaacacaaa ggtatcacat tacttagatt ggatgttttt
      361 gatgacagaa caagcgaatg tctttctgaa gagattaaga tataccaaga atgccatgaa
      421 aaattcgtta attttttaaa ggcaaacttc aaccaagaaa tatacccaca gctatatact
      481 cctgaaatat tttatgaggc ctgcagaaat ctccagtcat tttatgatca tcaggaaacg
      541 tcacaaaacg caaaatacag tgcaatagac aagaaaaaaa gtcatcttaa ccaggaaatc
      601 aaaaagctta ttaaaaaaaa tattttccct gagctataca atgagcaatg caataaaatc
      661 ccctcctcat cgatagatga taaccaggga ataatgtggc agaactttaa aactagcaat
      721 tcggcatatt caaaactatg tggaaaatta tctttattta aaagcgtgcc cagtcggtta
      781 atagaaaaat cagcttactg ctcaactgaa aacatggcca ctaataaatt taatgtcgcc
      841 ttttcttact gcggagataa tataaaggaa tttatacttt tattacccta taataaatca
      901 ctggagatgt atgaacttaa cgatcaaaag atccag
//
LOCUS       DAZPJR010000398          935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPJR010000398 DAZPJR010000000
VERSION     DAZPJR010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.406"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(197..>935)
                     /locus_tag="ACHVB7_004602"
     CDS             complement(197..>935)
                     /locus_tag="ACHVB7_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1916348.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          259 t
ORIGIN      
        1 ttgcttctcc gataaaatgt cagagaagca tgattacgtg gttattgtct gttgatgact
       61 tgttgaccga gccgtcatca ttctcaatgc attagttaaa tcatttgtga tttttaagga
      121 ttatcctgtc tatgattttc tacttagaag gatatggcac taactatgca tcttatagtt
      181 tactgctggc atctatttaa aatagcaatt gagtcaaata gcccatcaag atagccatac
      241 ctaatataac cgtcaggaat gcagctatca tcggcattct gaacattgat ttaagtaata
      301 tcacttccgt caggcttgcg ccggcactgc cgatgattaa tgccattaat gctcccagac
      361 ccattccttt tgtcatcaga acagatgcca gagggataac cgcctcagcc cggatataca
      421 gcggaatacc gacaacggcg cttaatggga tggcgaaggg attatctgct cctgcatgag
      481 cggcaatcca cgctgaggga ataaagccat aaataaaaga tcctattaat actcctaaca
      541 aaagatatgg cagtacatct ttaaactgtt gcaacgcatc tttggtggct agttttatgg
      601 gattaatact tactacagct ttggtactgc aacaggtaac tacggggttc tcaatggctt
      661 tagccgctgg actacaacaa ctcactttta tgggcgtata tgtcgttccc ggcgaagttt
      721 ttgctggagc acaacaattt gctgatgagc ttttactggc aatgatatga cgttcaaacc
      781 ccagagaatc caggataata ctggcaagaa cggagacgcc ggctgcgata atcgcgtaca
      841 acaaggtgac tttccaacca aaggtcatcc acattagtcc gacgataatg ggattaagta
      901 atggtgaaac aaataaaaaa gtgagagtcg gacca
//
LOCUS       DAZPJR010000399          922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPJR010000399 DAZPJR010000000
VERSION     DAZPJR010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.407"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHVB7_004603"
     CDS             complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHVB7_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR1916349.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          268 a          146 c          229 g          279 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtatgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaagtgttg acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct
      481 ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg
      541 tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga
      601 cattctttaa gtagggagac cgcttcattc cgattttcac cggggagcgc ttgtttttcc
      661 catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt ataaaaagaa
      721 ttttgggaca atgaaaagtt attatttatc ggtaacatga tggacattca gttaaagccg
      781 atggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt cattccatga
      841 aatgaacttc agcacttttt tattataaaa ggacaaacaa aattggctgt aaaaaaacga
      901 tactgtttca gttaactgtg aa
//
LOCUS       DAZPJR010000400          918 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPJR010000400 DAZPJR010000000
VERSION     DAZPJR010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.408"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..297)
                     /gene="lpfB-O113"
                     /locus_tag="ACHVB7_004604"
     CDS             complement(<1..297)
                     /gene="lpfB-O113"
                     /locus_tag="ACHVB7_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR1916350.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPS"
     gene            complement(345..917)
                     /gene="lpfA-O113"
                     /locus_tag="ACHVB7_004605"
     CDS             complement(345..917)
                     /gene="lpfA-O113"
                     /locus_tag="ACHVB7_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR1916351.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          261 a          216 c          189 g          252 t
ORIGIN      
        1 cgaaggttct aatcgtgata ctggcggaat aacggtaaac ggcgctttgc ttttatcctc
       61 tttattaaat ggatctaccc aagcctggat gagataaggt attttttctt ctttgctaaa
      121 tacagaaata gatttatcag cattattact taagtaaacc acccgcgtac caccaatgac
      181 aacgcctgca taagttaatg ctggttggcc taaaaaaacg ctgagtataa tcgcggtaaa
      241 taacttacat gaaactgttg ttttaaagag cctgattaaa caactttttc tggacataac
      301 gactccctgg ccaaagccaa ctgaataaaa aggcccttga taaattattt cttatattcg
      361 acagtaaatt gtgaatcagc gttcgccgta ccagccgtta cggtcgcatt ggttgccaca
      421 tagcgtgcaa taaacttcat ttcagccttg ccttcagcat cgatagcggt ataagcagag
      481 tcgttataaa gtttaacctg cgaattatcg gactggttaa aaatacgaat accaacgccg
      541 gttgccgcaa tcgcttgtcc tgtaccggta aaatcaccag ggttgcctgc tgtaagcggc
      601 gtacccactt tcaggtcgcc gtcgccgcca ggcgcttctg taccatcaaa acgaacggct
      661 gcttgagtgt acgttgccgg gcaattttca agtttaatac tgaacttctg tggcgacgcc
      721 agactaccta cacctgaaaa tgcattttta ctgactttac ccagtgggac aatttgatcg
      781 gctgtatcgg aggtaacttc acaagtagtg tcgacaattt caccgacgaa gtgaacaaca
      841 ccatcttctg ccagtgcatg cccggccagc attagagatg ccagagtgaa tgcaccgcct
      901 ataatattac gcttcata
//
LOCUS       DAZPJR010000401          875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPJR010000401 DAZPJR010000000
VERSION     DAZPJR010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.409"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>875
                     /gene="cadA"
                     /locus_tag="ACHVB7_004606"
     CDS             <1..>875
                     /gene="cadA"
                     /locus_tag="ACHVB7_004606"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR1916352.1"
                     /translation="FSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKG
                     DVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIK
                     RLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGM
                     EKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDF
                     KRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLP
                     TMVMTPYAAFQKELH"
BASE COUNT          237 a          220 c          212 g          206 t
ORIGIN      
        1 acttctcacc gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta gaagggaaag
       61 tgatttacga aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga
      121 tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca
      181 ccacttctcc tcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag
      241 gcaatgcagg taaacgtttg atcaacggtt ccattgaacg tgcgatcaaa ttccgtaaag
      301 agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtatgg cagccggatc
      361 atatcgatac gactgaatgc tggccgctgc gttctgacag cacctggcac ggcttcaaaa
      421 acatcgataa cgagcacatg tatcttgacc cgatcaaagt caccctgctg actccgggga
      481 tggaaaaaga cggcaccatg agcaactttg gtattccggc cagcatcgtg gcgaaatacc
      541 tcgacgaaca tggcatcgtt gttgagaaaa ccggtccgta taacctgctg ttcctgttca
      601 gcatcggtat cgataagacc aaagcactga gcctgctgcg tgctctgact gacttcaaac
      661 gtgcgttcga cctgaacctg cgtgtgaaaa acatgctgcc gtctctgtat cgtgaagatc
      721 ctgaattcta tgaaaacatg cgtattcagg aactggctca gaatatccac aaactgattg
      781 ttcaccacaa tctgccggat ctgatgtatc gcgcatttga agtgctgccg actatggtaa
      841 tgactccgta tgctgcgttc cagaaagagc tgcac
//
LOCUS       DAZPJR010000402          860 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPJR010000402 DAZPJR010000000
VERSION     DAZPJR010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.410"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..860
                     /locus_tag="ACHVB7_004607"
                     /pseudo
     CDS             1..860
                     /locus_tag="ACHVB7_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          239 a          210 c          230 g          181 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtctggtctg
      841 aaaacatcaa tgtcgcgtaa
//
LOCUS       DAZPJR010000403          857 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPJR010000403 DAZPJR010000000
VERSION     DAZPJR010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.411"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>857
                     /locus_tag="ACHVB7_004608"
     CDS             <1..>857
                     /locus_tag="ACHVB7_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFR1916353.1"
                     /translation="DLWPVKEPDMLTVIMQRLTALEQSGEMGRKMDAFKERVIRNSLR
                     PPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEGRVAGFESAYVALSRMKQHVQVYTDN
                     RQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLA
                     GGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKG
                     SGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVSIAGEGRPWNPGAITDGR
                     VWGDIPDNSV"
BASE COUNT          173 a          221 c          309 g          154 t
ORIGIN      
        1 gatctgtggc cggtaaagga gccggacatg ctgacggtga tcatgcagcg cctgacggca
       61 ctggagcagt ccggtgagat gggccggaaa atggatgcct ttaaggagcg ggtgatccgc
      121 aacagtctgc ggccccctgc cgtgcccggt atcggacgca cggagaaata cagcagccgg
      181 ttgtttgacc cgtccgtcag actggctgcg gatatccggg ataacgaagg gcgggtggcc
      241 ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca ggtgtacacc
      301 gataaccgtc agggctggac ggatgccatc aacaatgccg tacagaaagg aactgcccac
      361 gatgtgtttg agccgaaacc ggaccgggag gtcatgaatg cagagcggct gttcagtacg
      421 gcgcgggagc tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc ggggctggcc
      481 gggggagaca gtcctgcacg gtttattgct ccgggacgta aatatccgca accgtatgtg
      541 gcactgccgg cgtttgaccg taacggcaag tccgccggta tctggctgaa cccgctgacc
      601 acggatgacg gaaacgggct gcggggattc agcggtgaag gtcgggtaaa aggcagcggg
      661 gatgcgcagt tcgtggccct gcagggcagc cgtaacggag aaagcctgct ggctgataat
      721 atgcaggatg gcgtccggat tgcccgtgat aatcctgaca gtggtgtggt ggtaagcatt
      781 gccggtgaag gccgtccgtg gaatcccggt gccatcaccg atggccgcgt gtggggggat
      841 atcccggaca acagcgt
//
LOCUS       DAZPJR010000404          833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPJR010000404 DAZPJR010000000
VERSION     DAZPJR010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.412"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>833
                     /locus_tag="ACHVB7_004609"
     CDS             <1..>833
                     /locus_tag="ACHVB7_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1916354.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVA
                     KRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVE
                     TATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRW
                     L"
BASE COUNT          189 a          267 c          254 g          123 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg gttatccgca cggatgatga
      361 gcgcacccac cggtaccatt acgacagtca gcaccggctg gtgcactaca cgcggacaca
      421 atatgcagag ccgctggtcg aaagtcgcta tctttacgac ccgctgggcc gcagggtggc
      481 aaaacgggta tggcggcgtg aacgggacct gacgggctgg atgtcgctgt cacggaaacc
      541 gcaagtgacc tggtacggct gggacggcga ccggctgacc acaatacaga acgacagaac
      601 ccgcatccag acgatttatc agccggggag cttcacgcca ctcatcaggg tcgaaactgc
      661 caccggtgag ctggcgaaaa cgcagcgccg cagcctggcg gatgcccttc agcagtccgg
      721 cggcgaagac ggtggcagtg tggtgttccc gccggtgctg gtgcagatgc tcgaccggct
      781 ggaaagtgaa atcctggctg accgggtgag tgaggaaagc cgccgctggc tgg
//
LOCUS       DAZPJR010000405          795 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPJR010000405 DAZPJR010000000
VERSION     DAZPJR010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.413"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHVB7_004610"
     CDS             complement(<1..741)
                     /locus_tag="ACHVB7_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR1916355.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          266 a          137 c          161 g          231 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaagttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattt
//
LOCUS       DAZPJR010000406          793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPJR010000406 DAZPJR010000000
VERSION     DAZPJR010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.414"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            84..>793
                     /locus_tag="ACHVB7_004611"
     CDS             84..>793
                     /locus_tag="ACHVB7_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR1916356.1"
                     /translation="MALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETP
                     AQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQGPTGKESTVYGIY
                     GISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYK
                     DDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYY
                     SLAVMISAKQINNLISQE"
BASE COUNT          254 a          166 c          179 g          194 t
ORIGIN      
        1 ttatgttcac tctcttatct atatatttat aaggcaatta aatgaaaagg aatttattat
       61 cttccgctat tatagtcgcc atcatggccc tcggtctgac gggttgtgat gataaaaaag
      121 ccgaaacaga aacgcccccg cctgccaata gtcaacctgc cgcaccagct cctgaagctc
      181 ccgttgcaaa agcagaagct aaacctgaaa cacctgcgca accggtggtc gatgaacaag
      241 cggttttcga cgaaaaaatg gatgtctata tcaagtgcta caacaagtta cagatccctg
      301 tacagaacag tctggcgcgt tatgctgact ggctgaaaga ttttaaacag gggcctaccg
      361 gtaaagagag tactgtttat ggcatctacg gcattagtga atccaacctc gctgagtgtg
      421 aaaaaggcgt aaaaagtgct gtggcgttaa cgcctgcgct gcaacctatt gatggcgtgg
      481 cggtgagtta tattgatgct gccgtggcgt tgggtaatac cattaacgaa atggataaat
      541 attacaccca ggaaaattat aaagacgatg cgtttgcgaa agggaaaacg ctgcaccaga
      601 cattcttaaa aaatctggaa tcctttgaac ctgtagcaga atcttatcat gcggcgattc
      661 aggaaattaa tgataagcgc cagcttgcgg aactgaaaaa tattgaagaa agagaaggga
      721 aaacattcca ctactactct ctggcagtca tgatttcagc gaaacaaatt aataacctga
      781 tttcgcaaga gaa
//
LOCUS       DAZPJR010000407          790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPJR010000407 DAZPJR010000000
VERSION     DAZPJR010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.415"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            52..790
                     /locus_tag="ACHVB7_004612"
                     /pseudo
     CDS             52..790
                     /locus_tag="ACHVB7_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAZPJR010000408          778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPJR010000408 DAZPJR010000000
VERSION     DAZPJR010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.416"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            192..>778
                     /locus_tag="ACHVB7_004613"
     CDS             192..>778
                     /locus_tag="ACHVB7_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFR1916357.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKL"
BASE COUNT          174 a          236 c          200 g          168 t
ORIGIN      
        1 ccagccagac ccggcatgat tactgccccc agtcgtccat gatccggggg gtgatgtcac
       61 cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga
      121 ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca
      181 agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt atcggcgttg
      241 atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa cgcctgttcg
      301 ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac ctgaaacaac
      361 atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta cctgtcgccg
      421 ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg cgccgcatag
      481 ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc attgccgaag
      541 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
      601 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      661 accgtatccg cggccttctg acccagatac atccggcact ggagcgcgtt ctcggtccga
      721 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaact
//
LOCUS       DAZPJR010000409          776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPJR010000409 DAZPJR010000000
VERSION     DAZPJR010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.417"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..344
                     /locus_tag="ACHVB7_004614"
     CDS             <1..344
                     /locus_tag="ACHVB7_004614"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1916358.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(406..>776)
                     /locus_tag="ACHVB7_004615"
     CDS             complement(406..>776)
                     /locus_tag="ACHVB7_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR1916359.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          176 g          200 t
ORIGIN      
        1 gtgcagatgg tcatttttgt ggcaatttgc cgaatacgac ccatttccat gagcaaaccg
       61 tggagttccc gatccaggga gagatggttg atattgtggc gcatggtgag ttgggcggcg
      121 acttttcgtt agtgcctgac agctacgtga cgatggggcc gaaaagcatc atggcggcga
      181 aaaacttgct gataattgtc agtggcgcag gtaaagcgca ggcactgaaa aatgtgctgc
      241 aaggtccggt taccgaagat gttccagctt ccgtgttgca gctgcatcct tcgctgatag
      301 taatcgctga taaagccgct gccgcagaat tagcgctcgg ttaagctgaa gaaataaggc
      361 cggatgtatg aattacgctc cggcctgttc tcattattta aataactacg aaaaatcacc
      421 ccacagtctc agcttgccgc agtggagaca gcggaagaga taaccctgac aatcaccacc
      481 cttttgcagg catttagcta gatcagaatt tctaataccg aaattctcac aatcttcttc
      541 aaggttggca aattcatcga ggcgatcttt tatatcattc cagcccacat agccgataaa
      601 agcacagaaa tcgccacaat gcgcaagcca gaattcctgt tgccatccat gataacctgg
      661 cgtgcgttca accaactctt tcagcatttc atcaggatat gtgttcttaa taccggcaaa
      721 ttcgtcctct tcatcatact caaattcaac accttctatg ctggcatcat cctgaa
//
LOCUS       DAZPJR010000410          749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPJR010000410 DAZPJR010000000
VERSION     DAZPJR010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.418"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            584..>749
                     /locus_tag="ACHVB7_004616"
     CDS             584..>749
                     /locus_tag="ACHVB7_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR1916360.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVI"
BASE COUNT          212 a          135 c          164 g          238 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcg
//
LOCUS       DAZPJR010000411          745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPJR010000411 DAZPJR010000000
VERSION     DAZPJR010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.419"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..723
                     /locus_tag="ACHVB7_004617"
     CDS             <1..723
                     /locus_tag="ACHVB7_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1916361.1"
                     /translation="SNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPTNKPKNCQH
                     AVQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSHCWPWFKGD
                     ISSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKLAARIHDHD
                     RWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVREKLPTSGVI
                     MVTHQPGVWNLADDICDISTVL"
BASE COUNT          214 a          163 c          164 g          204 t
ORIGIN      
        1 tcgaacaatt taagctggtt tatttataaa tatgacgaac ttgctgaact ggctgcggtt
       61 atcgatcgct tgtatgagtt ccatcaactc actgaacagc gccctacgaa taagcctaaa
      121 aattgccaac atgcggtaca agtggctaat gcgagtattc gtacgcctga taataagatc
      181 atattagaga acttgaactt tcatgtttcg ccaggcaaat ggctattact gaaaggctac
      241 tctggtgcgg gaaaaaccac actgcttaaa acattatccc actgctggcc gtggtttaaa
      301 ggtgatattt cttctcctgc tgacagttgg tatgtgtcac aaacaccgtt aatcaaaagc
      361 ggcttactga aagagattat ttgtaaagca cttcccctgc ccgtagacga taaatcgttg
      421 agcgaagtac tgcatcaggt tggtctgggg aaattggctg cgcgtattca tgaccacgat
      481 cgctggggag atattctttc cagcggcgaa aaacaacgta tcgccctggc acgattaatt
      541 ttacgacgtc cgaaatggat atttcttgac gaaactacct ctcatcttga ggaacaagag
      601 gctatacgct tactgcgttt agtgcgtgaa aaactaccca caagcggcgt cattatggtt
      661 acacatcaac ccggtgtctg gaacctggct gatgatattt gtgacattag cacggtttta
      721 taacaacaat aaaagccctg ctgta
//
LOCUS       DAZPJR010000412          743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPJR010000412 DAZPJR010000000
VERSION     DAZPJR010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.420"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHVB7_004618"
     CDS             complement(<1..396)
                     /locus_tag="ACHVB7_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1916362.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          172 c          108 g          230 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttctcattaa cac
//
LOCUS       DAZPJR010000413          737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPJR010000413 DAZPJR010000000
VERSION     DAZPJR010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.421"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..688
                     /locus_tag="ACHVB7_004619"
     CDS             <1..688
                     /locus_tag="ACHVB7_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916363.1"
                     /translation="IHFITTHPSGIKDTLAWLVNASKLMTEFDDNGKIIFNINALKYT
                     KASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALSESIKGVKF
                     CIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFACVSFGF
                     PVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKWKRENMTPF
                     MYVLNTPPVI"
BASE COUNT          231 a          122 c          151 g          233 t
ORIGIN      
        1 cattcatttt atcactacac atccttcagg tataaaagat acactcgcct ggttagttaa
       61 cgcgagtaaa ttaatgactg agtttgatga caatgggaaa attatattta acattaatgc
      121 cttaaagtat acaaaagcaa gttactttga aatacttgga gagaaatata taaaaatcac
      181 aacatccagc ccctggcttt tagaaaagct cggtaaatat attttctctt cacgagcgcc
      241 tcaagttctg gaactcgcta ttggctggcg aggagcttta tctgaatcaa taaaaggagt
      301 taagttctgt atctggtttt cagtagcctg gagaaccata gaatttatta tgagttcaga
      361 gcgagatctg gttaattttc ttggcgattt ttcaatggat gtggccaaag cagttatagc
      421 cggtggtgtt gcaaccgcta ttggaagcct ggcttctttt gcctgtgtta gctttggctt
      481 tccagtaatt cttgtcggag gagcaatttt actgacaggg atagtgtgta cagttgtttt
      541 aaatgaaatc gatgctcaat gccatttatc agaaaaatta aaatatgcaa ttagagatgg
      601 actaaaacgg caacaggaac ttgataaatg gaaaagggaa aacatgactc catttatgta
      661 tgttcttaac actccacccg tgatatgagg attaaccatg cgtttacatg ttaaattaaa
      721 agagtttctt tcgatgt
//
LOCUS       DAZPJR010000414          735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPJR010000414 DAZPJR010000000
VERSION     DAZPJR010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.422"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>735
                     /locus_tag="ACHVB7_004620"
     rRNA            <1..>735
                     /locus_tag="ACHVB7_004620"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          168 a          164 c          241 g          162 t
ORIGIN      
        1 cggccttgaa ataccaccct ttaatgtttg atgttctaac gtggacccgt gatccgggtt
       61 gcggacagtg tctggtgggt agtttgactg gggcggtctc ctcctaaaga gtaacggagg
      121 agcacgaagg ttggctaatc ctggtcggac atcaggaggt tagtgcaatg gcataagcca
      181 gcttgactgc gagcgtgacg gcgcgagcag gtgcgaaagc aggtcatagt gatccggtgg
      241 ttctgaatgg aagggccatc gctcaacgga taaaaggtac tccggggata acaggctgat
      301 accgcccaag agttcatatc gacggcggtg tttggcacct cgatgtcggc tcatcacatc
      361 ctggggctga agtaggtccc aagggtatgg ctgttcgcca tttaaagtgg tacgcgagct
      421 gggtttagaa cgtcgtgaga cagttcggtc cctatctgcc gtgggcgctg gagaactgag
      481 gggggctgct cctagtacga gaggaccgga gtggacgcat cactggtgtt cgggttgtca
      541 tgccaatggc actgcccggt agctaaatgc ggaagagata agtgctgaaa gcatctaagc
      601 acgaaacttg ccccgagatg agttctccct gaccctttaa gggtcctgaa ggaacgttga
      661 agacgacgac gttgataggc cgggtgtgta agcgcagcga tgcgttgagc taaccggtac
      721 taatgaaccg tgagg
//
LOCUS       DAZPJR010000415          731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPJR010000415 DAZPJR010000000
VERSION     DAZPJR010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.423"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..>731)
                     /locus_tag="ACHVB7_004621"
     CDS             complement(<1..>731)
                     /locus_tag="ACHVB7_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR1916364.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAFL"
BASE COUNT          194 a          195 c          155 g          187 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
      661 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
      721 gattgctttc g
//
LOCUS       DAZPJR010000416          731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPJR010000416 DAZPJR010000000
VERSION     DAZPJR010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.424"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHVB7_004622"
     CDS             <1..239
                     /locus_tag="ACHVB7_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR1916365.1"
                     /translation="ADRNRSHWSTSKTPSYTKSVSWQHHPLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            344..535
                     /locus_tag="ACHVB7_004623"
     CDS             344..535
                     /locus_tag="ACHVB7_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR1916366.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(536..>731)
                     /locus_tag="ACHVB7_004624"
     CDS             complement(536..>731)
                     /locus_tag="ACHVB7_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1916367.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
BASE COUNT          189 a          185 c          161 g          196 t
ORIGIN      
        1 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
       61 taagttggca gcatcacccg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc
      121 aactcgatgc ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg
      181 tcctttaccg tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat
      241 ccaaaaagaa cccgtcggga tggcgggtta tttgtcctgg ttattccccc gttgtaaaat
      301 ctctcctaaa cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact
      361 ttttgtttgc tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga
      421 acagcagtgt ctttattcta tgagcgatca acggatccgc catggcggtt gttttccgat
      481 tgaggatttt atagatggtt tctggcgacc tgcacaggag tacggtgatt tttaattatt
      541 gcaattgcac aagagtcagt tcgcccccaa agacagcacc ggtatcaata taatgcaggt
      601 tgccaatatc cacgcgatgt cgcaacggtg tatgaccaaa ccagaaatga tcagcacctg
      661 taattccctg ccctttttgg cgttcaccta atcgcgagcg gctccacaag acctgatgca
      721 aatcaacgtc c
//
LOCUS       DAZPJR010000417          730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPJR010000417 DAZPJR010000000
VERSION     DAZPJR010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.425"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(162..>730)
                     /locus_tag="ACHVB7_004625"
     CDS             complement(162..>730)
                     /locus_tag="ACHVB7_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1916368.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQI
                     HLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          225 a          180 c          158 g          167 t
ORIGIN      
        1 gctttggctt gataaccgga taacaacgtg cgctttgttg atgccggatg cggcgtgaat
       61 tccttatccg gtctacaaaa tttgcaaatc caaagcatta cattaagacg tggcctgata
      121 aacatatcgc atcaggccat tttcgttagc tattgtccac gtcaggcttt cacatcactc
      181 actgcaccga ccggaatatc agcatgaaca ggctcgggcg gtactttgcg atatttatcc
      241 agcagatgaa tctggatcct gcgcagcgtg tcgccattga ggcgatagaa acggaagtag
      301 agaatcagcg ttaccatgaa aaacagagtt ggtagagcaa tcataataaa ctgcatacct
      361 aatagggctt gcgtagactg ttcaacattc ggtacatagc caatcatccc taacacaacc
      421 gcaatgaaaa aagccgcaaa ggctgaaccg cccttcacca ccatagtctg cacggagtaa
      481 gcgatacttt cacagcgtac gtgcagttta tattcaccgt aatcaacggt atctgccacc
      541 atgatgacct gtaataccca gaaaagcgcc gttcccacat tcagcaaaat acccgcaatc
      601 acaatgagga cgacgttgtg atagctcatt aatgccatta acaggagaac accacagctt
      661 aacaccggaa gaatagatgc tccggcccat aaaatgcgtc gggataatga tttaactaag
      721 cgtgggaaga
//
LOCUS       DAZPJR010000418          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPJR010000418 DAZPJR010000000
VERSION     DAZPJR010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.426"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHVB7_004626"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHVB7_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR1916369.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZPJR010000419          710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPJR010000419 DAZPJR010000000
VERSION     DAZPJR010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.427"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
BASE COUNT          245 a          109 c          147 g          209 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAZPJR010000420          707 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPJR010000420 DAZPJR010000000
VERSION     DAZPJR010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.428"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>707
                     /locus_tag="ACHVB7_004627"
     rRNA            <1..>707
                     /locus_tag="ACHVB7_004627"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          193 a          158 c          211 g          145 t
ORIGIN      
        1 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
       61 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcaaca
      121 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      181 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      241 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagtcccttg
      301 cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      361 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      421 taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
      481 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
      541 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
      601 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
      661 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtg
//
LOCUS       DAZPJR010000421          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPJR010000421 DAZPJR010000000
VERSION     DAZPJR010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.429"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHVB7_004628"
     CDS             <1..>695
                     /locus_tag="ACHVB7_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1916370.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZPJR010000422          690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPJR010000422 DAZPJR010000000
VERSION     DAZPJR010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.430"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            132..>690
                     /locus_tag="ACHVB7_004629"
     CDS             132..>690
                     /locus_tag="ACHVB7_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR1916371.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRL"
BASE COUNT          193 a          157 c          192 g          148 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga
//
LOCUS       DAZPJR010000423          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPJR010000423 DAZPJR010000000
VERSION     DAZPJR010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.431"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHVB7_004630"
     CDS             <1..560
                     /locus_tag="ACHVB7_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1916372.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..>684)
                     /locus_tag="ACHVB7_004631"
     CDS             complement(616..>684)
                     /locus_tag="ACHVB7_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916373.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DAZPJR010000424          677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPJR010000424 DAZPJR010000000
VERSION     DAZPJR010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.432"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>677
                     /locus_tag="ACHVB7_004632"
     CDS             <1..>677
                     /locus_tag="ACHVB7_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1916374.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQ"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAZPJR010000425          661 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPJR010000425 DAZPJR010000000
VERSION     DAZPJR010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.433"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..565)
                     /gene="ypdA"
                     /locus_tag="ACHVB7_004633"
     CDS             complement(<1..565)
                     /gene="ypdA"
                     /locus_tag="ACHVB7_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR1916375.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT          195 a          166 c          173 g          127 t
ORIGIN      
        1 gcacaataaa gccgatacag acgctaccga ggatcattgg aatgccgatt ttagagacga
       61 tatcgatccc caacgcggta gttggtgccc agacaattac cagaatcatg gtcaacgtct
      121 cacacaacat gccgccgaga atactgacgc gccagcgctg tgctttgggg attttcaggt
      181 ttatccagcc tgatatacaa cccgccagaa tgctggtgat aaagcagggg atcgccgtca
      241 cgccgccgat atcaattaaa taccggtgaa tacccgcaat cacgccggta atgatgccta
      301 cccacgggcc aaacagaatc ccgtcggaca tcaccgcgat aatacgtacg ttcaccagcg
      361 agccttctac gggaacgccg gaccaggtgc tgaacagggc gaacagcgag aaaatggcgg
      421 taacggcgag caattctttt ggggagtgcg ccgacttgtg caacagttcg cgaaacaggc
      481 ggatacggat gagaaagaac aggcagataa gcattaacgc tgcccgatcg aagaccgcca
      541 gcagcatgtt gaatatttcg tgcacgggtg actctgaata aggattaaac ctctatgata
      601 ggtaacctga aggctgatga ccagcaggcc gtttttgagg aaaaaccata aatttaaaag
      661 g
//
LOCUS       DAZPJR010000426          651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPJR010000426 DAZPJR010000000
VERSION     DAZPJR010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.434"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
BASE COUNT          206 a          155 c          127 g          163 t
ORIGIN      
        1 tcggaaagaa aaaaggccat cagcaaaact ctgatggcct gaatcacctt taccaatatt
       61 gtatgaaaaa acacgcacga ctaattgaca ataattttca tttccattaa aaaagatcgc
      121 gtagcattct taattcatga ggaactttac ccgccagcaa tctgagtagc gaaagctgtt
      181 catccccaat gtttttgctg gcgggtcctt ttttctacgg tcctcctccc cggagggggg
      241 catttcatta tttttctaat cgttactggt gagaaggcgg aagaaaaagc cagttcttcg
      301 cccctaaata acagcatgat tccagtccat gcaaaatctg atttccagct cacacatacc
      361 gagcgctgta caaaaaaccg ccagccaagg cagttaagag tgtgttcccg gggtttgctt
      421 agaatatttt gtaagttgtc cgaagagata tttacaacac cagaatgatg aatcatcagt
      481 cccctgccag aggttcacta cgtaacctga ccgacaatgc accattctgg tgctgaaaaa
      541 agagcactgg aactgcaaca aaacaacatc acagaacagg agatggtaag gagtcagaca
      601 ttcccacaca atattgtgtc agtgcaaata acaaccttcg tctcaaatct c
//
LOCUS       DAZPJR010000427          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPJR010000427 DAZPJR010000000
VERSION     DAZPJR010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.435"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="ACHVB7_004634"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="ACHVB7_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1916376.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAZPJR010000428          639 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPJR010000428 DAZPJR010000000
VERSION     DAZPJR010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.436"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(19..369)
                     /gene="traA"
                     /locus_tag="ACHVB7_004635"
                     /pseudo
     CDS             complement(19..369)
                     /gene="traA"
                     /locus_tag="ACHVB7_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(415..>639)
                     /locus_tag="ACHVB7_004636"
     CDS             complement(415..>639)
                     /locus_tag="ACHVB7_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR1916377.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          164 a          173 c          137 g          165 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaatccggc aacagacata cctactgcaa taaatacaga
       61 gagtatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgactgcacc
      121 gaccagaact tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgtt tcctgacctt tagccatcag gtccgtgcct ttaccagcag ccatagctaa
      241 ctcagggaac aacatcatca gaacagcaac cgggatcact gcacgaacca ggcgaagcat
      301 attcagacga gtgaatttag aaaaaaacga tttctttttg acgggcgcag aagtaccctg
      361 aacacttaac gctgaaccgc cccgggtttc ctggagagtg ttttatctgt gaactcaggc
      421 tgccagatca tcgtttccga tggaagcata ataagctttt tctgcttctg ccggaggagt
      481 atggcccagc ctttccagca atcgtcgatt gttataccag tccacccacg tgagtgtggc
      541 cagctccact tctgtccggt ttttccagct cttacggtgt attacctccg ctttgtaaag
      601 gcccttgatg atctctgcca tcgcgttgtc atacgagtc
//
LOCUS       DAZPJR010000429          631 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPJR010000429 DAZPJR010000000
VERSION     DAZPJR010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.437"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            151..606
                     /gene="ygeH"
                     /locus_tag="ACHVB7_004637"
                     /pseudo
     CDS             151..606
                     /gene="ygeH"
                     /locus_tag="ACHVB7_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
BASE COUNT          220 a          104 c          108 g          199 t
ORIGIN      
        1 cactgaattc ctaagataaa ttagtgaaca caaccataga tatgtcgaat gtattttcct
       61 gtagcattaa tgacacataa atatgaatag ccataatttc tattgcttaa gcaggatgca
      121 agaaaccaat tttttcatag aggttaacta atggacttag aaaataaatt ctcatatcat
      181 tttcttgagg gattaacgct cacggaagat ggaattctta ctcaaggaaa tgagcaagtt
      241 tatattccac agaaagagtt aggtgtatta atagtattac ttgaatccgc tggtcatgtt
      301 gtactgaaag atatgatcat cgaatcagta tggaaaaata ttattgttag tgacgagtcc
      361 ctgacaagat gtatctattc tttgcgctgc atttttgaaa aaattggcta tgatcgttgc
      421 atagaaacaa tctaccggaa aggttatcgt ttcagcgggc aggttttcaa aactaaaatt
      481 aatgaagata atacttcaga ctattccata gctatattcc ctttcactac ttcattgaat
      541 acactggatc cattaatact taatcaggaa ttagtgcaaa tcatttcaaa taaaaaaatc
      601 gatggttaga ctggccccct gaatctccag a
//
LOCUS       DAZPJR010000430          625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPJR010000430 DAZPJR010000000
VERSION     DAZPJR010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.438"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>625
                     /gene="istA"
                     /locus_tag="ACHVB7_004638"
     CDS             <1..>625
                     /gene="istA"
                     /locus_tag="ACHVB7_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR1916378.1"
                     /translation="KLTNRCIGLTLHISASTVFEVLARFKASSLSWPLPADISHDTLE
                     KLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQAGDKAMGYSHFCRCY
                     RKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVAIFVAVMGASNYTYV
                     EACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRYEPVI"
BASE COUNT          151 a          170 c          159 g          145 t
ORIGIN      
        1 aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac cgtttttgag
       61 gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga tatatcccat
      121 gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga gcttgttatg
      181 cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg ccaactgttg
      241 tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca cttctgccgt
      301 tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca tcgtgctggc
      361 gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc ggacaccgga
      421 gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta tacctatgtt
      481 gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg ttgcctgacc
      541 ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc agtgaaaaaa
      601 gccgatcgct atgagcctgt catca
//
LOCUS       DAZPJR010000431          620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPJR010000431 DAZPJR010000000
VERSION     DAZPJR010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.439"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..454
                     /gene="espX1"
                     /locus_tag="ACHVB7_004639"
     CDS             <1..454
                     /gene="espX1"
                     /locus_tag="ACHVB7_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFR1916379.1"
                     /translation="YYFCCVLTNISCFESDLSNTIFSNGEINNLFIKKSNIFGTSFTN
                     TMIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVR
                     DNVKLNNDVYKELNKFMHDYDKTSSKMRKYLQSINECMLLIKNIVHQD"
BASE COUNT          238 a          107 c           96 g          179 t
ORIGIN      
        1 gtattatttt tgttgtgtat taaccaatat atcatgtttt gaaagcgact tatcaaacac
       61 catcttttca aatggagaaa taaacaacct cttcattaaa aaaagcaaca tatttggaac
      121 atcgttcact aataccatga taaaaaattt gcgctgtgaa gatattatgc ctggccgatg
      181 gaccactcaa cttgtcaata agcacttagg ttacagatac acaggagtat tcaaaaccct
      241 ggcaagtatt gatgacaaac cgtcccgttt cgaaattttg ataccattgg tacaaacttt
      301 agttcgcgat aatgtaaagc taaataatga tgtgtataaa gaacttaata agtttatgca
      361 cgattacgac aaaacatcat caaaaatgag aaaatacctt cagagcatca acgagtgcat
      421 gctccttata aaaaatatag ttcatcagga ttaaaacact caaagaaaga ggtaatttta
      481 gaatgaaatt atctgtgatg attattaata cactgcagag aaataaaaaa caatctgaag
      541 catgctctga aacatcctac tctgtcactc taatgatggc accttgctct tcttataaag
      601 aagggaacaa ttaagaatat
//
LOCUS       DAZPJR010000432          614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPJR010000432 DAZPJR010000000
VERSION     DAZPJR010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.440"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHVB7_004640"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHVB7_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1916380.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(420..>614)
                     /locus_tag="ACHVB7_004641"
     CDS             complement(420..>614)
                     /locus_tag="ACHVB7_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1916381.1"
                     /translation="LPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISCEVTFRHGTL
                     RFNGNVSEKLLTLLIQELKR"
BASE COUNT          136 a          165 c          175 g          138 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc cgtgccggaa cgttacctca cagctgatac tgagggtttt cgggtcctct
      541 gcgagcgatt ctggctgttc ggcagctgca tcgagagtca caggaagtag ctgggggctc
      601 tctgaagaag gtaa
//
LOCUS       DAZPJR010000433          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPJR010000433 DAZPJR010000000
VERSION     DAZPJR010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.441"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHVB7_004642"
     CDS             <1..173
                     /locus_tag="ACHVB7_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916382.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..>593
                     /locus_tag="ACHVB7_004643"
     CDS             186..>593
                     /locus_tag="ACHVB7_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR1916383.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKE"
BASE COUNT          157 a          114 c          166 g          156 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 ataaaaaaat taactgtctg gtcggtaaaa cgccggctgg tttcagcagt gaacagattc
      541 acgcaacgct tgtagaaaac ctgcgttatg ccgcgaatat gctgatgaaa gaa
//
LOCUS       DAZPJR010000434          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPJR010000434 DAZPJR010000000
VERSION     DAZPJR010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.442"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHVB7_004644"
     CDS             <1..91
                     /locus_tag="ACHVB7_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916384.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHVB7_004645"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHVB7_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1916385.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZPJR010000435          575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPJR010000435 DAZPJR010000000
VERSION     DAZPJR010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.443"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
BASE COUNT          157 a          113 c           91 g          214 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaatatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgtttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgt
//
LOCUS       DAZPJR010000436          574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPJR010000436 DAZPJR010000000
VERSION     DAZPJR010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.444"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..516)
                     /locus_tag="ACHVB7_004646"
     CDS             complement(<1..516)
                     /locus_tag="ACHVB7_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1916386.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          160 a          137 c          115 g          162 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttac
//
LOCUS       DAZPJR010000437          571 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPJR010000437 DAZPJR010000000
VERSION     DAZPJR010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.445"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHVB7_004647"
     CDS             <1..246
                     /locus_tag="ACHVB7_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR1916387.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(286..>571)
                     /locus_tag="ACHVB7_004648"
     CDS             complement(286..>571)
                     /locus_tag="ACHVB7_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1916388.1"
                     /translation="RQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFR
                     CQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT          109 a          150 c          169 g          143 t
ORIGIN      
        1 gctgccctgc ccgtaagttg gatccagatg ctggcaggtc tggcgctgtt gagtaccatt
       61 ggcggcagtt tgtatcaggc gctgcataat gagcgtgagc gtgagcgcga cgcggcggtg
      121 gtggcatttc tggtaacggc aagtggattg acgctggtcg ggattggttc tgcgttctgg
      181 ggattaattg ccggaggcgt ttgttacgtg gtgttgaatt taatcgctga cagaaaccga
      241 tattgacatc ctccacgccc tgaaggacgt ggatccctgc tacgttcagg ctgtcgcctg
      301 aatcatttcg gtgggttcct gcttcaacgg gcggcctgac tgcaccatct ctccacaggc
      361 aagaacggcg tgccccgccg ctaaaatgtt acgagcacca tttacatcgg cgttcgctgt
      421 atatccacat acctggcatc tgaatttact ttgtgacagg cggttctctt tcgctgtatg
      481 accacagtac gcgcaacgct ggcttgtata cgctggcgga acagcaagca cctgaccgcc
      541 acgccagagc tgcttgtact caagctggcg g
//
LOCUS       DAZPJR010000438          570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPJR010000438 DAZPJR010000000
VERSION     DAZPJR010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.446"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..439
                     /locus_tag="ACHVB7_004649"
     CDS             <1..439
                     /locus_tag="ACHVB7_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR1916389.1"
                     /translation="DIEKQLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFK
                     SGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWIT
                     ELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          168 a          124 c          159 g          119 t
ORIGIN      
        1 ggatattgaa aaacagctta ccagcgtggc gaggcagaat gaaacctgta agcggttgct
       61 ggatattcct ggcgttggac cacttattgc gacggcagcg gtggccacca tgggggaagc
      121 atcagcgttt aaatcggggc gagagttcgc cgcatatgtt ggtctggttc caaaacaaac
      181 tggctccgga gggaaagtac gtctgctggg gataagcaaa cgtggtgaca cttatctcag
      241 gacattattt atccacggtg caagagcggt ggcattagta gctaaagagc ctggcccgtg
      301 gataaccgaa ctgaaaaaac gtcgtccagc cagtgtggca atcgtcgcca tggcaaacaa
      361 gctggcacga acagtatggg cgataaccgc ccatgaccgt aagtatgaca ggaaccacgt
      421 cagtatcaga ccatattaat cgctgatacc attaaacaat gaactcttaa caaaagggtg
      481 aatgctgaaa ggttgctatg gcggccagag tgatgacaaa gacaggtaag accgtgactc
      541 actaaacctg aacagtattt tgggcttgaa
//
LOCUS       DAZPJR010000439          566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPJR010000439 DAZPJR010000000
VERSION     DAZPJR010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.447"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..225
                     /locus_tag="ACHVB7_004650"
     CDS             <1..225
                     /locus_tag="ACHVB7_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR1916390.1"
                     /translation="DSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            267..467
                     /locus_tag="ACHVB7_004651"
                     /pseudo
     CDS             267..467
                     /locus_tag="ACHVB7_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          174 a          128 c          142 g          122 t
ORIGIN      
        1 gactcgtatg acaacgcgat ggcggagagc atcaatggtc tttacaaagc ggaggtaata
       61 caccgtaaga gctggaaaaa ccgtgcagaa gtggaactgg ccatactcac gtgggtggac
      121 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      181 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
      241 actctccagg aaacccgggg cggttcaacc ttcacgataa aaaacttttc attaaaggta
      301 aagcggatca atatccggca cttcattctc tgatatctcc gacccgcatt aatctgaaag
      361 aaatagaaat tcactggcgt gaagtgctac gtctggcgac ctcaataaaa cagggaacag
      421 tgacagcatc cctgatgctg agaaaacttg ccagttaccc caaacaaaag taaggcagta
      481 tgactttccg tgagacagat gctggccaac gtgggatatt taacgcgggt taacatcgtt
      541 gtcagagccg catgggtgag ctgttg
//
LOCUS       DAZPJR010000440          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPJR010000440 DAZPJR010000000
VERSION     DAZPJR010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.448"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHVB7_004652"
     CDS             <1..>557
                     /locus_tag="ACHVB7_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR1916391.1"
                     /translation="IPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARL
                     RELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPDYHVEY
                     ARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHR
                     RHGTWTPERLIEQGNRTGPSTGRVV"
BASE COUNT          142 a          169 c          148 g           98 t
ORIGIN      
        1 tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa gcagaaaacg gcgtcctcat
       61 cgtcgagcga tggcttcttg cccgcatccg taatgaaacc ttccacacgc tcagggcgct
      121 gaatgcacgc cttcgggagt tgctgacgga catgaataac cgaccaatga agggatacgg
      181 taatcaaacc cgcgcagaac gcttccgcat gctggatgca ccagcactct ctccgctgcc
      241 tctggagcct tatgaataca cggaatacaa agccgtgaag gttggccctg actaccacgt
      301 tgaatatgcc cgtcactggt actccgttcc acacgaactg gtcggtcagc gtctgtcact
      361 gaaggtgggg cagtcggtgg tacagctctg gcacaagggg cagtgtgtgg cgcaacatcc
      421 gcgcagcaca catgaatata aacacacaac caatccgctt cacatgccag aacgccaccg
      481 gcggcatggg acatggacac cggaacgcct tatcgagcag ggaaaccgta caggtccgtc
      541 cacaggccgg gtggttg
//
LOCUS       DAZPJR010000441          555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPJR010000441 DAZPJR010000000
VERSION     DAZPJR010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.449"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            38..>555
                     /locus_tag="ACHVB7_004653"
     CDS             38..>555
                     /locus_tag="ACHVB7_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1916392.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVT"
BASE COUNT          161 a          113 c          134 g          147 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaac
//
LOCUS       DAZPJR010000442          552 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPJR010000442 DAZPJR010000000
VERSION     DAZPJR010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.450"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            5..211
                     /locus_tag="ACHVB7_004654"
     CDS             5..211
                     /locus_tag="ACHVB7_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1916393.1"
                     /translation="MEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGSLLL
                     GLLTYLTNLYFKIREDKRKAARGE"
     gene            211..>552
                     /locus_tag="ACHVB7_004655"
     CDS             211..>552
                     /locus_tag="ACHVB7_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1916394.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNF"
BASE COUNT          146 a          125 c          145 g          136 t
ORIGIN      
        1 tcagatggaa aaaatatcaa caggcattgc ctacggcacc tccgccggca gtgccggcta
       61 ctggtttttg cagtggttgg atcaggtcag tccatcacag tgggctgcga ttggtgtgct
      121 gggaagtctg cttctggggc ttctgactta tctgacaaat ctgtatttca aaataagaga
      181 agacaagcgt aaggctgcga gaggtgaata atgtcgccat cattacgcaa ggctgttgca
      241 gctgctattg gtggtggggc tgttgctata gcatctgtgt taatcactgg cccaggtggt
      301 aacgatggtc tggaaggtgt cagctacata ccatacaaag atatcattgg tgtatggact
      361 gtatgtcacg gacacaccgg aaaagacatc atgctcggta aaacgtatac cgaagcagaa
      421 tgcaaagccc tcctgaataa agaccttgcc actgtcgcca gacaaattaa cccgtacatc
      481 aaagtcgata taccggaaac aacgcgcggc gctctttact cgttcgttta caacgtgggc
      541 gctggcaatt tc
//
LOCUS       DAZPJR010000443          550 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPJR010000443 DAZPJR010000000
VERSION     DAZPJR010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.451"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>550
                     /gene="feaR"
                     /locus_tag="ACHVB7_004656"
     CDS             <1..>550
                     /gene="feaR"
                     /locus_tag="ACHVB7_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR1916395.1"
                     /translation="QEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDASR
                     PCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNNP
                     ALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIA
                     GETGMSVRSLYRMFADKGLVVA"
BASE COUNT          139 a          125 c          146 g          140 t
ORIGIN      
        1 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
       61 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
      121 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
      181 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
      241 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
      301 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
      361 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg ccgtgaacgt cagtttcaaa
      421 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
      481 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
      541 tagtcgcgca
//
LOCUS       DAZPJR010000444          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPJR010000444 DAZPJR010000000
VERSION     DAZPJR010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.452"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHVB7_004657"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHVB7_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZPJR010000445          540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPJR010000445 DAZPJR010000000
VERSION     DAZPJR010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.453"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>540
                     /locus_tag="ACHVB7_004658"
     CDS             <1..>540
                     /locus_tag="ACHVB7_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1916396.1"
                     /translation="TILSDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTS
                     YQVAYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNS
                     LSANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDE
                     NNKEEERFNLFISIPFYWG"
BASE COUNT          167 a          105 c          108 g          160 t
ORIGIN      
        1 tacgatttta tccgataact ataatgctat aactctggga aatggttgga atacaccgct
       61 gggtgccatc tctttttacg ctgcccgatc gagtagtaag ttaaataatg ataccagaca
      121 cgaaggtacg agctatcagg tcgcctataa taaatatcta gtacaaacag ccactcgatt
      181 tagcgtggcc gcctggcgtt atgcttcgca ggattacagg acattcagcg accatcttta
      241 cgaaaatgat aaacactatc atcagagtga ttatgatgat ttttatgata ttggcagaaa
      301 aaatagcctt tctgccaata ttatgcaacc tttatccaat aatctgggaa atgtatcatt
      361 aagtgcgctt tggcggaatt actgggggcg aagtggaaat gctaaagatt accaattcag
      421 ttactccaat aactggcaac acattagtta tactttctct gccagccaat cttatgatga
      481 aaataataaa gaagaggagc gttttaatct gtttatctcc attcctttct actgggggga
//
LOCUS       DAZPJR010000446          520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPJR010000446 DAZPJR010000000
VERSION     DAZPJR010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.454"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>520
                     /locus_tag="ACHVB7_004659"
     CDS             <1..>520
                     /locus_tag="ACHVB7_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR1916397.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAZPJR010000447          509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPJR010000447 DAZPJR010000000
VERSION     DAZPJR010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.455"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..>509)
                     /gene="espL1"
                     /locus_tag="ACHVB7_004660"
     CDS             complement(<1..>509)
                     /gene="espL1"
                     /locus_tag="ACHVB7_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR1916398.1"
                     /translation="LDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALLRHPPA
                     GSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSVYGQLNYVV
                     SVYDPNDTNVAVRGTHRAARGFLSLDKFISSAPDAQTWADRYVRNCAIAILPLLPEGV
                     PVAILAGITT"
BASE COUNT          149 a          115 c          117 g          128 t
ORIGIN      
        1 gtcgtgatac ccgctaaaat agcaactgga actccctcag gtaatagagg aagaatagca
       61 attgcacagt tgcgaacata cctatcagcc caggtctgag catcgggagc tgaactgatg
      121 aacttatcaa gcgagagaaa gccccgtgct gccctgtggg tgcctctgac ggcaacgttg
      181 gtatcgttcg ggtcgtaaac agacacaaca taatttaatt gtccatacac tgactgcttt
      241 attcgtagcc ttactgacaa agtatgattg tctatatata aaatcgcgga catatgactg
      301 acattgtttg ccatcattcg acaaaagaaa atatctaaag cgaagccaaa atccttactc
      361 gcgataacgc gactccctgc aggaggatgg cgaagtaatg ctctgaactc cgcgtcccgg
      421 actaagggga catgctgctg tatggattca acggaggagt agtgtttaat caggttgact
      481 ttgccttttc catttctcaa tgaatcaag
//
LOCUS       DAZPJR010000448          507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPJR010000448 DAZPJR010000000
VERSION     DAZPJR010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.456"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..188)
                     /locus_tag="ACHVB7_004661"
     CDS             complement(<1..188)
                     /locus_tag="ACHVB7_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1916399.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="ACHVB7_004662"
                     /pseudo
     CDS             242..445
                     /locus_tag="ACHVB7_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          126 a          136 c          117 g          128 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggattcc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaac
//
LOCUS       DAZPJR010000449          504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPJR010000449 DAZPJR010000000
VERSION     DAZPJR010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.457"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHVB7_004663"
     CDS             <1..194
                     /locus_tag="ACHVB7_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1916400.1"
                     /translation="LKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKIV
                     VKYNNWITKDNGEFELAVH"
     gene            224..>504
                     /locus_tag="ACHVB7_004664"
     CDS             224..>504
                     /locus_tag="ACHVB7_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR1916401.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          172 a           96 c          107 g          129 t
ORIGIN      
        1 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
       61 agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa tcttgaaagc atatctggct
      121 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      181 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      241 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      301 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      361 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      421 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
      481 cccggcgcaa ctggttcaaa gcct
//
LOCUS       DAZPJR010000450          503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPJR010000450 DAZPJR010000000
VERSION     DAZPJR010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.458"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>503
                     /locus_tag="ACHVB7_004665"
     CDS             <1..>503
                     /locus_tag="ACHVB7_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1916402.1"
                     /translation="PGETDIALPAPLPFILSRTYSSYRTKTPAPMESLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHRE"
BASE COUNT           92 a          162 c          161 g           88 t
ORIGIN      
        1 ttcccggtga aaccgacatc gccctgcccg ccccgctgcc gttcatcctc tcccgcacct
       61 acagcagtta ccggacaaaa acgcccgcgc cgatggagag cctcggcccc ggctggaaaa
      121 tgcctgcgga tatccgctta cagctgcgcg ataacacact gatactcagt gataacggcg
      181 gcagaagcct gtattttgag cacctgtttc ccggtgagga cggttacagc cgcagcgaat
      241 cactctggct ggtgcgcggc ggcgtggcga aactggatga aggtcaccgg ctggccgcac
      301 tctggcaggc gctgccggaa gaactccgct taagtccgca tcgttatctg gcgacaaaca
      361 gtccgcaggg gccgtggtgg ctgctcggct ggtgtgagcg ggtgccggaa gcggatgagg
      421 tgctgcctgc gccgctgccg ccgtaccggg tactgaccgg gctggtggac cgcttcgggc
      481 gcacacagac gttccaccgc gaa
//
LOCUS       DAZPJR010000451          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPJR010000451 DAZPJR010000000
VERSION     DAZPJR010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.459"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHVB7_004666"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHVB7_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR1916403.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZPJR010000452          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPJR010000452 DAZPJR010000000
VERSION     DAZPJR010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.460"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHVB7_004667"
     rRNA            <1..>476
                     /locus_tag="ACHVB7_004667"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZPJR010000453          475 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPJR010000453 DAZPJR010000000
VERSION     DAZPJR010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.461"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..>475)
                     /locus_tag="ACHVB7_004668"
     CDS             complement(<1..>475)
                     /locus_tag="ACHVB7_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR1916404.1"
                     /translation="VVYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLIGFSVGM
                     SWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFTPIFLPI
                     AQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLPFYAS"
BASE COUNT          163 a          108 c           98 g          106 t
ORIGIN      
        1 tgctagcata aaatggcagc aatggtttga ttaaatgttg aatcttcaca cccgagatag
       61 aacaaccaac aaatagcgca ctacccaccg gcggtgttaa cagcccaata caaaggttgg
      121 ccaccatcat aataccgaaa tgtactgggt ccattcccag ctcttgtgca atcggcaaga
      181 agatcggtgt aaagattagt acagccggtg tcatgtccat aaagatgccg acaattaata
      241 agacgatatt aatcagcagc aatatgatca gtgggttatc agaaatcccc atcaatgcgt
      301 cgctgatcat ataaggaata tcagcattag tcattgccca ggacatgccc acggaaaaac
      361 cgatcaacag taatacaatc gaagtggtaa caaccgactc cagaatcagt ttgggtaaat
      421 ggcgccattt tacttcccgg taaatcagca ccgagagaat aaatgtgtag acaac
//
LOCUS       DAZPJR010000454          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPJR010000454 DAZPJR010000000
VERSION     DAZPJR010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.462"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            89..448
                     /locus_tag="ACHVB7_004669"
                     /pseudo
     CDS             89..448
                     /locus_tag="ACHVB7_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAZPJR010000455          462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPJR010000455 DAZPJR010000000
VERSION     DAZPJR010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.463"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            12..>462
                     /locus_tag="ACHVB7_004670"
     CDS             12..>462
                     /locus_tag="ACHVB7_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR1916405.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          113 a          108 c          121 g          120 t
ORIGIN      
        1 cggaggccag catgaacaac aataacacat tgtacgtcgg gttagatgtt cacaaagaat
       61 cgattactgt cgcttatgct atcaattcag aacctgttga actgatgggt aaaattggca
      121 catcacctac tgatattcag aatctttgta aacgtctcag gtcaaagtca tcgcaggtca
      181 gtatcgttta tgaagcgggg ccctgtggtt atggacttta tcgtcggctg gtgaaatccg
      241 gttttgactg catggtctgc gccccctcac ttatcccgaa aaaaccgggg gagcgggtta
      301 aaaccgatcg ccgtgatgcc atcagacttg tgcgttcact gcgtgcagga gatctttctg
      361 ctgtctacgt acccggcatt gaagatgagg cattccggga tttggcccgg gcatgggcat
      421 ctgcccgcga tgatttacgg catgcaaggc aacgtctgaa at
//
LOCUS       DAZPJR010000456          461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPJR010000456 DAZPJR010000000
VERSION     DAZPJR010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.464"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>461
                     /locus_tag="ACHVB7_004671"
     CDS             <1..>461
                     /locus_tag="ACHVB7_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1916406.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAZPJR010000457          454 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPJR010000457 DAZPJR010000000
VERSION     DAZPJR010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.465"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..386
                     /locus_tag="ACHVB7_004672"
     CDS             <1..386
                     /locus_tag="ACHVB7_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1916407.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           95 c          122 g          104 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agca
//
LOCUS       DAZPJR010000458          450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPJR010000458 DAZPJR010000000
VERSION     DAZPJR010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.466"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            71..146
                     /locus_tag="ACHVB7_004673"
     tRNA            71..146
                     /locus_tag="ACHVB7_004673"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
     gene            340..>450
                     /locus_tag="ACHVB7_004674"
     rRNA            340..>450
                     /locus_tag="ACHVB7_004674"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          124 a           97 c          118 g          111 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt gagtctctcg aattttcgca
      301 acacgatgat gaatcgaaag aaacatcttc gggttgtgag gttaagcgac taagcgtaca
      361 cggtggatgc cctggcagtc agaggcgatg aaggacgtgc taatctgcga taagcgtcgg
      421 taaggtgata tgaaccgtta taaccggcga
//
LOCUS       DAZPJR010000459          449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPJR010000459 DAZPJR010000000
VERSION     DAZPJR010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.467"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>449
                     /locus_tag="ACHVB7_004675"
     CDS             <1..>449
                     /locus_tag="ACHVB7_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1916408.1"
                     /translation="QRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFNSE
                     TVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPE
                     SNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSA"
BASE COUNT          111 a          108 c          145 g           85 t
ORIGIN      
        1 tcagcgatgg tgctctgacg ggttcgagtt ccgctgtgat aacggagaaa aactgcgagt
       61 cacgttcgcg ctggactgct gtgaccgtga ggcactgcac tgggcggtca ctacgggcgg
      121 cttcaacagt gaaacagtac aggacgtcat gctgggagcg gtggaacgcc gcttcggcaa
      181 cgagcttccg gcgtctccag tagagtggct gacggataat ggttcatgct accgggctaa
      241 tgaaacacgg cagtttgccc ggatgttggg gcttgaaccg aagagcacgg cggtgcggag
      301 tccggagagt aacggcatag cagagagctt cgtgaaaacg ataaagcgtg actacatcag
      361 tgtcatgccc aaaccagacg ggttaacggc agcaaagaac cttgcagagg cgttcgagca
      421 ttataacgaa tggcatccgc atagtgcac
//
LOCUS       DAZPJR010000460          444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPJR010000460 DAZPJR010000000
VERSION     DAZPJR010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.468"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>444
                     /locus_tag="ACHVB7_004676"
     rRNA            <1..>444
                     /locus_tag="ACHVB7_004676"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          123 a           90 c          139 g           92 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggca
//
LOCUS       DAZPJR010000461          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPJR010000461 DAZPJR010000000
VERSION     DAZPJR010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.469"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            281..>442
                     /locus_tag="ACHVB7_004677"
     CDS             281..>442
                     /locus_tag="ACHVB7_004677"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR1916409.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGI"
BASE COUNT          170 a           90 c           64 g          118 t
ORIGIN      
        1 cattaaatca attaaatcac ttaaacctgc aggtcatttc gtaattacat taattaaaaa
       61 acttaaaacc attaaataaa taaaaaacca ctaactctat gtgaaataaa tcaaaatttc
      121 acgctgcaat actccttaga atgtatagcg aaaggagaaa aagatatacc tcgatcaccc
      181 ccctttctcc caagtgaaaa taaaaggtta tcaacttgca acattgaaca acattcgttg
      241 caaatcgata acaacatgca ccttcaggat actatttatt atgttcggca atgatatttt
      301 cacccgcgta aaacgttcag aaaataaaaa aatggcggaa atcgcccaat tcctgcatga
      361 aaatgatttg agcgttgaca ccacagtcga agtatttatt accgtaaccc gcgatgaaaa
      421 gcttatcgcg tgcggtggaa tt
//
LOCUS       DAZPJR010000462          436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPJR010000462 DAZPJR010000000
VERSION     DAZPJR010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.470"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>436
                     /locus_tag="ACHVB7_004678"
     CDS             <1..>436
                     /locus_tag="ACHVB7_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1916410.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEV"
BASE COUNT          127 a          113 c          122 g           74 t
ORIGIN      
        1 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
       61 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      121 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      181 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      241 atggataaag gttattactc actgggactg ttaaatgcct ggagcctggc gggagaacac
      301 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      361 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      421 ctgggaaatg aagtga
//
LOCUS       DAZPJR010000463          435 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPJR010000463 DAZPJR010000000
VERSION     DAZPJR010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.471"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            30..206
                     /locus_tag="ACHVB7_004679"
     CDS             30..206
                     /locus_tag="ACHVB7_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916411.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          157 a           50 c          105 g          123 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagagatgg tggcaggcca
      421 gggaaagatc atcag
//
LOCUS       DAZPJR010000464          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPJR010000464 DAZPJR010000000
VERSION     DAZPJR010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.472"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHVB7_004680"
     tRNA            41..116
                     /locus_tag="ACHVB7_004680"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZPJR010000465          419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPJR010000465 DAZPJR010000000
VERSION     DAZPJR010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.473"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
BASE COUNT          102 a          122 c           68 g          127 t
ORIGIN      
        1 agcaccttcg cacccagcac cgggttcacc gggttagcat aggtaatccc tcccggacac
       61 accgaacagg ccactcccgt cggcgcacct atcagcacac ccgctgaacc ctgcacaata
      121 tccagtccct tgcgggtcat gtcgccctgc cgcgccgccg gttttccgct catggtttat
      181 tcctcttcat ctgaattgat ttacattagg ttggttattt tctcataccc acaactatat
      241 tttctctctg aatttattac gtgcaagaca atcgcagcac tctaactaac ttcgatgcaa
      301 tttgatgttc agaaaaatcc ctcattagat tattatcatg aaatacagct aatcatcttt
      361 agcttatata tttctcaatg tattcttttg aaaggatccg tgccctttca aactcttca
//
LOCUS       DAZPJR010000466          416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPJR010000466 DAZPJR010000000
VERSION     DAZPJR010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.474"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..231)
                     /locus_tag="ACHVB7_004681"
     CDS             complement(<1..231)
                     /locus_tag="ACHVB7_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1916412.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPL"
BASE COUNT          109 a           92 c           92 g          123 t
ORIGIN      
        1 taacggactg tctctccctt gtcgcccatt aggctgatgg tgtgttatat cggtatcaac
       61 cttatttggg ttgatataga aagcctccgg aaccaccctg taactcctcc cgtgtagttc
      121 aggtgcagga taaatgtttc cattccttgc ccatcgtctc agcgttgata ttgatggtgg
      181 gttatccgga tatctgagtt ttccccacgt tttgagtgtc acaagattca ttgccatacc
      241 tctcacgata tgaccgccag taaatataca gaatactggc gggtgtggtt gatttttaat
      301 aatcagctat gtgaggtagc ctgagtttaa cggacactcc ttcctgaaat agaatggcat
      361 cagaaggagc taataatgag cagaaaaacc caacgttact ctaaagagtt caaagc
//
LOCUS       DAZPJR010000467          408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPJR010000467 DAZPJR010000000
VERSION     DAZPJR010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.475"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHVB7_004682"
     tRNA            54..130
                     /locus_tag="ACHVB7_004682"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHVB7_004683"
     tRNA            173..248
                     /locus_tag="ACHVB7_004683"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAZPJR010000468          403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPJR010000468 DAZPJR010000000
VERSION     DAZPJR010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.476"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            18..125
                     /locus_tag="ACHVB7_004684"
     CDS             18..125
                     /locus_tag="ACHVB7_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1916413.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           98 a           92 c          128 g           85 t
ORIGIN      
        1 acaatacagg cggctatatg acgctcgcag aactgggcat ggccttctgg catgatttag
       61 cggctccggt cattgctggt attcttgcca gtatgatcgt gaactggctg aacaagcgga
      121 agtaacgtgc catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg gatcaggccg
      181 caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca agcggataat
      241 attctgcgga atgcgcagca cagagtgaag acgtgacgga ttccggaaca gaaaaacagt
      301 cgtagaatca gcgtcaagcc ggaagggttc cggtgaggcg caatgttgcg ggggctttat
      361 ccctggtggc attggttgct ggagagagaa aacccccgca cgt
//
LOCUS       DAZPJR010000469          401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPJR010000469 DAZPJR010000000
VERSION     DAZPJR010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.477"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..356)
                     /locus_tag="ACHVB7_004685"
     CDS             complement(<1..356)
                     /locus_tag="ACHVB7_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1916414.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTA"
BASE COUNT          105 a          101 c          118 g           77 t
ORIGIN      
        1 tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga
       61 ataacggcac tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
      121 acaatctggt gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc
      181 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
      241 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      301 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      361 gcgtttccgg attcttacac agccactgga tcggtcaact g
//
LOCUS       DAZPJR010000470          400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPJR010000470 DAZPJR010000000
VERSION     DAZPJR010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.478"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..392
                     /locus_tag="ACHVB7_004686"
     CDS             <1..392
                     /locus_tag="ACHVB7_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFR1916415.1"
                     /translation="CIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGL
                     GISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWF
                     FLQRHKDFAFKPFFTNFNTIERIENYL"
BASE COUNT          145 a           74 c           55 g          126 t
ORIGIN      
        1 aatgtatcga aaatggcatt aattcaactc cctcgacagg ataccaacct cttatcctgc
       61 aaagcattaa aaccaaatta ctttcatcaa gactaaataa atttaatgac ttcgaggaca
      121 gttttaatgg cttaggaatc agtgttcatg atatttcagc tcaaaaaata tctcttttaa
      181 gttttcaaaa atacgctata ggatggagtg caacaattca ttttgtagca caggaccact
      241 tcggtcttga tgtaactgat atcaaaaaca agacttacag caaatataga ttttttcgca
      301 tatggttctt tttacaacgg cataaagact ttgctttcaa accttttttc accaatttta
      361 acactatcga aagaatcgaa aattatctat gaaaagcaaa
//
LOCUS       DAZPJR010000471          399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPJR010000471 DAZPJR010000000
VERSION     DAZPJR010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.479"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>399
                     /locus_tag="ACHVB7_004687"
     CDS             <1..>399
                     /locus_tag="ACHVB7_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR1916416.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGMT"
BASE COUNT          105 a           65 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatgac
//
LOCUS       DAZPJR010000472          398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPJR010000472 DAZPJR010000000
VERSION     DAZPJR010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.480"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..>398)
                     /locus_tag="ACHVB7_004688"
     CDS             complement(<1..>398)
                     /locus_tag="ACHVB7_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR1916417.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           84 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatactt
//
LOCUS       DAZPJR010000473          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPJR010000473 DAZPJR010000000
VERSION     DAZPJR010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.481"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
BASE COUNT          126 a          104 c           67 g           98 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcata
//
LOCUS       DAZPJR010000474          394 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPJR010000474 DAZPJR010000000
VERSION     DAZPJR010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.482"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            63..>394
                     /gene="yhaV"
                     /locus_tag="ACHVB7_004689"
     CDS             63..>394
                     /gene="yhaV"
                     /locus_tag="ACHVB7_004689"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR1916418.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          120 a           72 c          100 g          102 t
ORIGIN      
        1 ggaaagaaac ttgtcactgg catggacgtc aacattgatg atgagattgg cgacgacgaa
       61 taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg
      121 aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt
      181 atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata
      241 tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa
      301 ataaagactg gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata
      361 gtgaaaaaga gaaagtcatc attctgggat ggat
//
LOCUS       DAZPJR010000475          392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPJR010000475 DAZPJR010000000
VERSION     DAZPJR010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.483"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>392
                     /locus_tag="ACHVB7_004690"
     CDS             <1..>392
                     /locus_tag="ACHVB7_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1916419.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCP"
BASE COUNT          141 a           54 c           65 g          132 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc tt
//
LOCUS       DAZPJR010000476          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPJR010000476 DAZPJR010000000
VERSION     DAZPJR010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.484"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>389
                     /locus_tag="ACHVB7_004691"
     CDS             <1..>389
                     /locus_tag="ACHVB7_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916420.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTAA"
BASE COUNT           82 a           99 c          103 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccgcag
//
LOCUS       DAZPJR010000477          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPJR010000477 DAZPJR010000000
VERSION     DAZPJR010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.485"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            251..>389
                     /locus_tag="ACHVB7_004692"
     CDS             251..>389
                     /locus_tag="ACHVB7_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFR1916421.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT          121 a           69 c           65 g          134 t
ORIGIN      
        1 ctaaacttaa taaaacctta agattaacat tttaatataa cgagttgcat ttagttacat
       61 gtttaacact tgatttaaga tttgtaatgg ctagattgaa atcagatgta atccattagt
      121 tttatatttt acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca
      181 gtacttctgg tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg
      241 gggtttcatc atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac
      301 taaaccttct ttgcaggcga cggctctctt gcagcacctt aaacaatcgc tggcaattac
      361 aggaaaatta cataatattc aacgttctc
//
LOCUS       DAZPJR010000478          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPJR010000478 DAZPJR010000000
VERSION     DAZPJR010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.486"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHVB7_004693"
     CDS             <1..>374
                     /locus_tag="ACHVB7_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1916422.1"
                     /translation="SRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQ
                     IRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPH
                     EYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          106 a           87 c          111 g           70 t
ORIGIN      
        1 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
       61 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      121 tggcgatacc agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca
      181 atggaacgct tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc
      241 ttcagcgagg cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga
      301 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac
      361 tctaactcgg tggc
//
LOCUS       DAZPJR010000479          369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPJR010000479 DAZPJR010000000
VERSION     DAZPJR010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.487"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(166..>369)
                     /locus_tag="ACHVB7_004694"
     CDS             complement(166..>369)
                     /locus_tag="ACHVB7_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR1916423.1"
                     /translation="NKCLIEATLKGFKSGELSFYEYRKNLATAGVFRWITNIHENKRY
                     YYTFDNSLLFTENIQNTTQIFPH"
BASE COUNT           98 a           72 c           84 g          115 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttatt
//
LOCUS       DAZPJR010000480          366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPJR010000480 DAZPJR010000000
VERSION     DAZPJR010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.488"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>366
                     /locus_tag="ACHVB7_004695"
     CDS             <1..>366
                     /locus_tag="ACHVB7_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1916424.1"
                     /translation="EQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAA
                     YAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQGKR
                     HNQALIALARRRCDVLFAM"
BASE COUNT           66 a          125 c           99 g           76 t
ORIGIN      
        1 agaacagcga gttcttgctc accctcttta cccggtcctg accagtatgc ccggagtcgg
       61 tgtcaggacc gcagccagac tcctcaccga ggtcgcctgc cgcgccttcg cctctgccgc
      121 acatctcgct gcttatgctg gccttgcgcc ggtaactcgg cgatccggct cgtcaatacg
      181 cggtgagcat ccctcgcgac ggggtaataa agctctcaaa cgggcgttgt tcctgtcggc
      241 cttcgccgcg ctcagggatc cgctctccag ggcttactac acccgcaaaa tgagtcaggg
      301 aaaacgacac aatcaggcgc ttatcgccct ggcgagacga cgctgcgacg ttctgttcgc
      361 catgat
//
LOCUS       DAZPJR010000481          358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPJR010000481 DAZPJR010000000
VERSION     DAZPJR010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.489"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..>358)
                     /locus_tag="ACHVB7_004696"
     CDS             complement(<1..>358)
                     /locus_tag="ACHVB7_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1916425.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQY"
BASE COUNT          117 a           56 c           50 g          135 t
ORIGIN      
        1 aatattgcat tcttttatta aaacaccaat ccagtattct tacatcatta atatatgggg
       61 attttaaaaa agtctccaga atcgaatcga tgaccggtgt aaggttaact tttgcatcat
      121 ctaaggaatc aataagaata tgagacaggt taactttgat gtcattaatt tcattgtcag
      181 tagctttctt attattaaga agataattat ttactgcgtt aaggtcatct atttctttca
      241 gtatcccttt ttcaccaagc aagtatgaaa tcgattcatt acatacataa ggcattaata
      301 gcagatgttc tttccttatt gctaataatg cattgttcaa tggtttttct aataaatc
//
LOCUS       DAZPJR010000482          351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPJR010000482 DAZPJR010000000
VERSION     DAZPJR010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.490"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..305)
                     /locus_tag="ACHVB7_004697"
     CDS             complement(<1..305)
                     /locus_tag="ACHVB7_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1916426.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT          106 a           74 c           75 g           96 t
ORIGIN      
        1 cttgaatcct ctctcggcac ggttgcaatt agcgagtggg tagtatcacc aacagcgcga
       61 aatccgcgtc tgataatgga tatgttctga tataacgtgt tagtaggcac acgcatacct
      121 tcttcttccc agactctggt gaaaaaatct tgttgtgcaa ccacatcagg aaaagcttca
      181 agtagaagtg ataaacatcg actggctggc gttgtcagaa cgacgttaag atcggcgtta
      241 tgtaccgata ttaattttcg gtgctcaggc cagaactcga tattatcgtt aataatccag
      301 tacatacgtc aataaacact aaatcaatcg aaatggagat cacatagttt g
//
LOCUS       DAZPJR010000483          339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPJR010000483 DAZPJR010000000
VERSION     DAZPJR010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.492"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..261
                     /gene="istB"
                     /locus_tag="ACHVB7_004698"
     CDS             <1..261
                     /gene="istB"
                     /locus_tag="ACHVB7_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR1916427.1"
                     /translation="LDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKWHGL
                     MENPTTADAILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
BASE COUNT          108 a           74 c           93 g           64 t
ORIGIN      
        1 ctggacgatc tggggctgga acagttaagt aacgcccagt gtaatgacct tctggagatc
       61 acagaggatc gttacggaca aagcagcacg atagtggtaa gccagttccc ggtagataaa
      121 tggcatggtc tgatggagaa cccaacaaca gcagatgcca tcctggacag actggtacat
      181 aactcacaca gagtggtcct tcagggagag tccctgagga aaaatccccc gacagtggaa
      241 agcagcgaaa aaacgagtta aaaagcggta gaaaaagtta agttcccggt tgatcgaatg
      301 gatgcgaatc gctgatcgaa cttcgatgcc aatccgtga
//
LOCUS       DAZPJR010000484          339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPJR010000484 DAZPJR010000000
VERSION     DAZPJR010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.493"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..>339)
                     /locus_tag="ACHVB7_004699"
     CDS             complement(<1..>339)
                     /locus_tag="ACHVB7_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1916428.1"
                     /translation="CDQSKDFSCDQSKIKIAETLFFNLDKEPYKNSRKLQELIWDKLV
                     AYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKWYFY
                     QLLKCYEGEQ"
BASE COUNT          118 a           61 c           46 g          114 t
ORIGIN      
        1 gctgttcgcc ctcataacac ttaagtaatt gatagaagta ccacttagca aaatcaggtt
       61 gcttcatgaa aaaatccttc aaaaaaatat catttactaa aatgctgaca ggcacttcat
      121 taaatagtaa cttaacatca tcaaataacc ttagtattaa gcgtgatttt tcttgattgc
      181 ttaagctaaa atcattgaca tatgcaacca acttatccca aatcaattcc tgtaatttac
      241 ggctattttt atagggttct ttatcgagat tgaaaaataa ggtctcagct attttaattt
      301 tagattgatc gcagctaaaa tctttagatt gatcgcagc
//
LOCUS       DAZPJR010000485          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPJR010000485 DAZPJR010000000
VERSION     DAZPJR010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.494"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..229)
                     /locus_tag="ACHVB7_004700"
     CDS             complement(<1..229)
                     /locus_tag="ACHVB7_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFR1916429.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAP"
     gene            complement(226..>332)
                     /locus_tag="ACHVB7_004701"
     CDS             complement(226..>332)
                     /locus_tag="ACHVB7_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1916430.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT           83 a           97 c           84 g           68 t
ORIGIN      
        1 ccggggcaag ttccggcatc tcattacaca acagcacggg gtcaatcact cctgaaacac
       61 gggcctgtct gcagatgcct gtgtagatat cccgatacgc tcgtacagaa aaaatgtccg
      121 ccggtagtgt gatcagaata tccatcactt catgatctgc cccacgcaga aagaatgcgc
      181 caatgacagc gccttccagg tcatcattac gccagactgg tgttgtcatg cagccacacc
      241 tctgatacaa gaacggtagc tgggccagtt gaacgacaac cagttgcgcc ccccgtctgt
      301 gatcctgtcg gcaatgcggg ggctgatgaa cg
//
LOCUS       DAZPJR010000486          330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPJR010000486 DAZPJR010000000
VERSION     DAZPJR010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.495"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
BASE COUNT          103 a           62 c           46 g          119 t
ORIGIN      
        1 ttatttcctg aaaaatatct aaaaacatca acatctatat ttctattcat ttccttcata
       61 ttaagtgcaa tagtagcata ttttgttttc cataagcata aatctgccac tctatccata
      121 tgcagcttag tgttcttagt gttaattgta ggtgctaacg tagggtttat cagttacagc
      181 gacctaacac aaaaagcgac gcaaaaatta gatagtttgt ctaaataatt atgttgccat
      241 gcgaagtatg catggctgca tgtctgcctt ccatttaaaa tgggctaaga cctataaccc
      301 taaatattat tctttattat cttctttacc
//
LOCUS       DAZPJR010000487          329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPJR010000487 DAZPJR010000000
VERSION     DAZPJR010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.496"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            66..>329
                     /locus_tag="ACHVB7_004702"
     CDS             66..>329
                     /locus_tag="ACHVB7_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1916431.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
BASE COUNT           98 a           65 c           84 g           82 t
ORIGIN      
        1 tttcaccata ttatagtttt atttgtgaat taaatcaatt atggcgatga attacaaggg
       61 gttaaatgct gccgcagcat agcgatattg aaatagcctg gtatgcttcg atacagcagg
      121 agccgaatgg ctggaagacc gtcaccacac agttctacat ccaggaattc agtgagcata
      181 ttgcgccact tcaggatgct gtagatctgg agatcgcaac ggaggaagaa aactcgttgc
      241 tggaagcctg gaagaagtat cgggtgttgc tgaaccgtgt tgatacatca actgcacctg
      301 atattgagtg gccggaagaa ccagacaca
//
LOCUS       DAZPJR010000488          312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPJR010000488 DAZPJR010000000
VERSION     DAZPJR010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.497"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(1..76)
                     /locus_tag="ACHVB7_004703"
     tRNA            complement(1..76)
                     /locus_tag="ACHVB7_004703"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Thr,seq:tgt)
     gene            complement(79..155)
                     /locus_tag="ACHVB7_004704"
     tRNA            complement(79..155)
                     /locus_tag="ACHVB7_004704"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Arg,seq:tct)
     gene            complement(157..233)
                     /locus_tag="ACHVB7_004705"
     tRNA            complement(157..233)
                     /locus_tag="ACHVB7_004705"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ile,seq:cat)
BASE COUNT           71 a           87 c           81 g           73 t
ORIGIN      
        1 tggtgccgac taccggaatc gaactggtga cctactgatt acaagtcagt tgctctgcct
       61 gctgagctaa gtcggcgctg gcccaccacc gaggactcga acctcgcacc gtcaacttag
      121 aaggttgatg ctctatccgg atgagctagt ggtggttggt ggcccttgct ggacttgaac
      181 cagcgacctg gcgattatga gtcgctcgct ctgaccaact gagctaaagg gccggaggca
      241 gaataataac catatgtcat cacatctgca aactcatctg accaccagca cgtttaacgt
      301 cctgtgccgt tt
//
LOCUS       DAZPJR010000489          311 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPJR010000489 DAZPJR010000000
VERSION     DAZPJR010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.498"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
BASE COUNT           99 a           58 c           61 g           93 t
ORIGIN      
        1 cacaagaatg ttaaatttaa taaacaaaaa gttatttcgc tgtatgataa aaaccacccg
       61 ttataattta ttagtgaaat tcgtttttcg agtgttagaa atttatatct aaatagcgtt
      121 gattaatgag caaagccaga ctcctgtaac gctcacaaga ctcatctacc tgcgacgggt
      181 attctggtca gtagtagatg tttaaggcgt ggcagagaca tttcatcctt actctacggc
      241 attggcccgc atacaaatcg tggttgtgac actcacttat catcagtgag cgaacagaga
      301 atagttcagt g
//
LOCUS       DAZPJR010000490          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPJR010000490 DAZPJR010000000
VERSION     DAZPJR010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.499"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHVB7_004706"
     CDS             <1..>301
                     /locus_tag="ACHVB7_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916432.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAZPJR010000491          293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPJR010000491 DAZPJR010000000
VERSION     DAZPJR010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.500"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>293
                     /locus_tag="ACHVB7_004707"
     CDS             <1..>293
                     /locus_tag="ACHVB7_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1916433.1"
                     /translation="DRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMN
                     GGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEW"
BASE COUNT           78 a           56 c           81 g           78 t
ORIGIN      
        1 gatcgtgggc cgcaaagttt acgttgtctg cagttactgg aacaacattg ggtttgtgcg
       61 gtaagaggca atcatgaaca gatggcgatg gatgcgctgg catcccagca gatgtctttg
      121 tggttgatga atggcggcga ctggtttatt gcgctggcag ataatcaaca gaaacaagcg
      181 aaaacggcgc tggaaaaatg tcagcatttg ccctttattc ttgaagtaca cagtcgtacc
      241 ggcaaacatg ttattgctca tgccgattat ccagatgatg tttatgaatg gca
//
LOCUS       DAZPJR010000492          288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPJR010000492 DAZPJR010000000
VERSION     DAZPJR010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.501"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
BASE COUNT           80 a           65 c           63 g           80 t
ORIGIN      
        1 gcccgaggta tattctgaaa tatcattcat catgaccggc gtttatacaa acgccggtca
       61 ttcgctaaca gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg
      121 ttcataagcg tattatcagg attgtctgga aaattcccga tactttggac tattactcct
      181 gaacaattcg aaattcagca gattaaaggt aagcgttaac ggaggtaagc gttaacggag
      241 gtaagcgtaa accgactgcc gtatgtagcc attaagccag tattggta
//
LOCUS       DAZPJR010000493          283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPJR010000493 DAZPJR010000000
VERSION     DAZPJR010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.502"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>283
                     /locus_tag="ACHVB7_004708"
     CDS             <1..>283
                     /locus_tag="ACHVB7_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR1916434.1"
                     /translation="RLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQVHH
                     FAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGA"
BASE COUNT           72 a           55 c           87 g           69 t
ORIGIN      
        1 cgacttatca gtggaaaaac acagaacgat caggatatat gcgccctggc aaaagagtat
       61 gagatcgcca tgctgttggt gactaaaggt gcagaagggg tggtggtctg ttatcgagga
      121 caagttcacc attttgctgg aatgtctgtg aattgtgtcg atagcacggg ggcgggagat
      181 gcgttcgttg ccgggttact cacaggtctg tcctctacgg gattatctac agatgagaga
      241 gaaatgcgac gaattatcga tctcgctcaa cgttgcggag cgc
//
LOCUS       DAZPJR010000494          279 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPJR010000494 DAZPJR010000000
VERSION     DAZPJR010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.503"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(50..>279)
                     /gene="trbI"
                     /locus_tag="ACHVB7_004709"
     CDS             complement(50..>279)
                     /gene="trbI"
                     /locus_tag="ACHVB7_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR1916435.1"
                     /translation="QTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHA
                     VILVSPAVVQGAPDITREIQQDIARRMRAEP"
BASE COUNT           51 a           88 c           73 g           67 t
ORIGIN      
        1 gcggccacac tctgccccca tatcagcagg gcaatcagcc cccggcatct catggttccg
       61 ctctcattcg ccgggcaata tcctgctgga tttcccgggt gatatccgga gccccctgta
      121 ccacggcagg cgacaccaga atgaccgcgt ggtgtttctg ctgccacgcc tgcaggctgg
      181 cttccagtgc cgtgttaaaa cgcgctgaaa gggcttttga ctgtgcttct gacagttgtt
      241 tctgactggc gctgtcaaaa aatgcatcca cagtctgtt
//
LOCUS       DAZPJR010000495          272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPJR010000495 DAZPJR010000000
VERSION     DAZPJR010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.504"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>272
                     /gene="hlyE"
                     /locus_tag="ACHVB7_004710"
     CDS             <1..>272
                     /gene="hlyE"
                     /locus_tag="ACHVB7_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR1916436.1"
                     /translation="NKYLDQVIPWQTFDETIKELSRFKQEYSQAASVLVGDIKTLLMD
                     SQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILI"
BASE COUNT           85 a           53 c           58 g           76 t
ORIGIN      
        1 ataataaata tctcgatcag gtcatcccct ggcagacctt tgatgaaacc ataaaagagt
       61 taagtcgctt taaacaggag tattcacagg cagcctccgt tttagtcggc gatattaaaa
      121 ccttacttat ggatagccag gataagtatt ttgaagcaac ccaaacagtg tatgaatggt
      181 gtggtgttgc gacgcaattg ctcgctgcgt atattttgct atttgatgag tacaatgaga
      241 agaaagcatc cgcccagaaa gacattctca tt
//
LOCUS       DAZPJR010000496          268 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPJR010000496 DAZPJR010000000
VERSION     DAZPJR010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.505"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..>268
                     /locus_tag="ACHVB7_004711"
     CDS             <1..>268
                     /locus_tag="ACHVB7_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916437.1"
                     /translation="TEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRL
                     QDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
BASE COUNT           90 a           69 c           52 g           57 t
ORIGIN      
        1 aacagaggcc accctgtacg aaaacgggct gtatacgcgc cacatcagac tcagtcgccc
       61 agtatctgac agcaaatacc ccttgccacc cggatctcag ctcattaaaa atatgacctt
      121 acgactgcgc ctgcaggatc agcaggaaga aacatccgtg acattatttc agggaaaaat
      181 gagtaccgac ggaaataact attacatcat caaaggtaaa gtaagctcag tacttctgtt
      241 attaaaaatg ctgcagatta atcacgcc
//
LOCUS       DAZPJR010000497          262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPJR010000497 DAZPJR010000000
VERSION     DAZPJR010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.506"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            82..>262
                     /locus_tag="ACHVB7_004712"
     CDS             82..>262
                     /locus_tag="ACHVB7_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1916438.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGK"
BASE COUNT           80 a           63 c           62 g           57 t
ORIGIN      
        1 gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta
       61 gtgggaacca aagtgtcaga tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc
      121 cctaattatc ctcccgaatt taaacagcag ctcgttgctg cctcctgtga acccgggata
      181 tccatctcaa aacttgctct tgaaaatggc attaacgcca atctgttgtt caaatggcga
      241 caacaatggc gcgagggaaa gc
//
LOCUS       DAZPJR010000498          259 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPJR010000498 DAZPJR010000000
VERSION     DAZPJR010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.denovo.507"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            2..>259
                     /gene="istB"
                     /locus_tag="ACHVB7_004713"
     CDS             2..>259
                     /gene="istB"
                     /locus_tag="ACHVB7_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR1916439.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQ"
BASE COUNT           74 a           61 c           76 g           48 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacag
//
LOCUS       DAZPJR010000499        85817 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.guided.002, whole genome shotgun sequence.
ACCESSION   DAZPJR010000499 DAZPJR010000000
VERSION     DAZPJR010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 85817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85817
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.guided.002"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(80..601)
                     /locus_tag="ACHVB7_004714"
     CDS             complement(80..601)
                     /locus_tag="ACHVB7_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1916440.1"
                     /translation="MRTLDITYGIPAEVWPRDYEHAERTLLFWRSAKFPVRVTLEDGQ
                     VFCMYVYGLLSARNKLNLTPDPLSNTNRIRLPLERISTIESGIEEVDTSFTGRLTLNN
                     EQMVNQPSRRDFFKICRQAYQNGKSIRVYMADGREIEGTSDGVDACLVTLKLEDGRKI
                     LIFFDWVERILPF"
     gene            complement(598..1191)
                     /locus_tag="ACHVB7_004715"
     CDS             complement(598..1191)
                     /locus_tag="ACHVB7_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916441.1"
                     /translation="MKRSLLLACIYLSSFSANALSDIVSDMQKTYSMYTSEDVLKYES
                     RGLIQPVWFRFSDNAEETILNAGKERLRLQINGTAVPENVNVSSDEAMKPSGIVLEVQ
                     DGTLYCDEMATTGCQVNIRVGNEEPIVTYAKAEGNTSGKVLFIDENTSKRIVRDIQKT
                     ANSKSVDKKIYVEVPFFVVGNKEFKFGLLKTPLRVSE"
     gene            complement(1373..1651)
                     /locus_tag="ACHVB7_004716"
     CDS             complement(1373..1651)
                     /locus_tag="ACHVB7_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916442.1"
                     /translation="MSEKKTTYCQVALSDKANDKLGKFQMKLKEKNIKMSKAEVINTI
                     LETMTMAEFDKVSSAVGVSAKAREKIMRIYENSNMTKEDLEQILSKLP"
     gene            complement(1654..3213)
                     /locus_tag="ACHVB7_004717"
     CDS             complement(1654..3213)
                     /locus_tag="ACHVB7_004717"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1916443.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQLVQQMLSYEVEAGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAKNGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQATRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            3296..3976
                     /locus_tag="ACHVB7_004718"
     CDS             3296..3976
                     /locus_tag="ACHVB7_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR1916444.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEALVWL
                     VDYYKERM"
     gene            3976..4644
                     /locus_tag="ACHVB7_004719"
     CDS             3976..4644
                     /locus_tag="ACHVB7_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFR1916445.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            4641..5279
                     /locus_tag="ACHVB7_004720"
     CDS             4641..5279
                     /locus_tag="ACHVB7_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1916446.1"
                     /translation="MSKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYA"
     gene            5272..5526
                     /locus_tag="ACHVB7_004721"
     CDS             5272..5526
                     /locus_tag="ACHVB7_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916447.1"
                     /translation="MPDLKIVELKPSKETDNNNVEVIRLLEEALQHAREGKSQSLALL
                     MINNDGSVLDCWHNGGRPYVMVGAMESLRLDFINANIERR"
     gene            5532..6422
                     /locus_tag="ACHVB7_004722"
     CDS             5532..6422
                     /locus_tag="ACHVB7_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916448.1"
                     /translation="MADIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            6432..6698
                     /locus_tag="ACHVB7_004723"
     CDS             6432..6698
                     /locus_tag="ACHVB7_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916449.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
     gene            6895..7527
                     /locus_tag="ACHVB7_004724"
     CDS             6895..7527
                     /locus_tag="ACHVB7_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1916450.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDE"
     gene            7527..8783
                     /locus_tag="ACHVB7_004725"
     CDS             7527..8783
                     /locus_tag="ACHVB7_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFR1916451.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQVTVFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMSASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            8810..10384
                     /locus_tag="ACHVB7_004726"
     CDS             8810..10384
                     /locus_tag="ACHVB7_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1916452.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGEHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDRGAAQESG
                     QDQPRGNGDSSTQENE"
     gene            10406..11293
                     /locus_tag="ACHVB7_004727"
     CDS             10406..11293
                     /locus_tag="ACHVB7_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6651 domain-containing protein"
                     /protein_id="HFR1916453.1"
                     /translation="MNLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTQEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENTRREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERYAALE
                     SENASLKSQLVEMTVGASFSNSAFLRDKVLMTPAKARVIYGSHFEVGDDGSVVGYDKP
                     AGHKDRAVLVDGEGNTLSFESAIERIVRADPEADAVMRSEAKQGAGSNSKPTHKVVQQ
                     KSKSTMDKLTSGLGKIGLK"
     gene            11319..12239
                     /locus_tag="ACHVB7_004728"
     CDS             11319..12239
                     /locus_tag="ACHVB7_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1916454.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREATLSEATFIDVNDTIAEGAATFSEHVAKLRILAGDVDVDKFLATTMSDTNNQL
                     AVQVRQKVKGLARAFRRNLILGNSETNTKAFDGIPRLMHKDQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGIPFIVND
                     FIPKNGVKVTEDSEQKTEGQEGTANIYCLHLSEENGLTGLYGGDNAGIVVENIGTVQN
                     KDAVRTRVKWYCSLANKHDKAIAALNNVKI"
     gene            12337..12771
                     /locus_tag="ACHVB7_004729"
     CDS             12337..12771
                     /locus_tag="ACHVB7_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916455.1"
                     /translation="MKPAKIRLLEPQFSDYSGMLCGVKFENGVSVSELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYGERNDLNVDQIVEPSAPDIVPMKRGTADEPAKPIQTFTRE
                     ELESIADSEGIAGLRLIGNKIGVKAKGIVEMIDGIMKAQGGE"
     gene            12771..13604
                     /locus_tag="ACHVB7_004730"
     CDS             12771..13604
                     /locus_tag="ACHVB7_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916456.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITSGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNTFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDIVAAREDGILSESIGETTNMYRTGKSANVHVARSTWRLLIRYIDNRMIVRR
                     S"
     gene            13676..14020
                     /locus_tag="ACHVB7_004731"
     CDS             13676..14020
                     /locus_tag="ACHVB7_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916457.1"
                     /translation="MNISWQTEIAIYRFGTKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLESAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            14011..14484
                     /locus_tag="ACHVB7_004732"
     CDS             14011..14484
                     /locus_tag="ACHVB7_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916458.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIEGVKPRVGPHFLERAVEISEKQFSEAILEAAKKAGFTRG"
     gene            14486..14869
                     /locus_tag="ACHVB7_004733"
     CDS             14486..14869
                     /locus_tag="ACHVB7_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFR1916459.1"
                     /translation="MFIEAFANLMQKAKIGTVGTDIFCHYLPAYVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTIEKARKIIDMFPVEETESGEVYFRLVRPMAMP
                     VVYPKNDGSLIEAGIPVEFAGYLLN"
     gene            14943..15680
                     /locus_tag="ACHVB7_004734"
     CDS             14943..15680
                     /locus_tag="ACHVB7_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFR1916460.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QQGQTVISELVQGRNITITAPLAESVLKNMVDLMPGSTLSEDDNSVTITSAQGVNLIN
                     VAKELVLTPQDTTDYVLTLPKAATAGNFTMSYQSDDVRVFSVQFNAYPDDEGVLGKMS
                     GPKPVKNVTITPESPSVKVGATVQLTANFTPDDVVDKTGVWSSDNKKATVDHTGLVRG
                     VEQGSANISFTSNSGSKKVTKLVTVNQ"
     gene            15748..16065
                     /locus_tag="ACHVB7_004735"
     CDS             15748..16065
                     /locus_tag="ACHVB7_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916461.1"
                     /translation="MTKLLDLDSILPPKKSVKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LTNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            16191..16415
                     /locus_tag="ACHVB7_004736"
     CDS             16191..16415
                     /locus_tag="ACHVB7_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916462.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARAAQADGEGVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDRADPDAKEQLMQIFGRG"
     gene            16423..20979
                     /locus_tag="ACHVB7_004737"
     CDS             16423..20979
                     /locus_tag="ACHVB7_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR1916463.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAVGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASASVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLINKPVRVRTSGSGNGGGSGGR
                     GGYSWHIGKNNEGSMFSGLRGNIFLLGEIGDAARTVTSIMFGWQKPIIEAAAEMERMR
                     VMLRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLK
                     ALVDSVARFGGDSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGI
                     TMGELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFAD
                     RVGQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQEL
                     VINLGKVVAAMVAFKLMRAGIMGVVGAGSKMVSTFATMATAIQTPFNLGATAVTRFNR
                     AARMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMT
                     MLRSETSKVVDEIRKIPEAMTAAKRAQMADRAEKLEKQILADQRALKTGEGVNYVSNS
                     AGVITHKESKADIEARLKKNQEEYQKITGTIALGDGAVAKRLAKEAAESQIEKIRAEN
                     QIFAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVDSF
                     SSRKNATEKQIATLNDQLEKAKKDGNTEQIQKLQGSIRGYQEHLEAVAQELTQAEFER
                     DNAAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMDFE
                     GKPIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQRS
                     ANASQRAAKKEQTAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLT
                     DLANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPESDSIIS
                     AGYTPSREEKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTALIK
                     ETGTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDIT
                     NMVVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSVGE
                     ATKETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSM
                     QNLSSVTGALSAAFAMLGANASGSGNKWLSFGATVASGLVSAWAGGGFDSLTSSSAKT
                     ATSSVADGTKGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGSMNEAY
                     VPLPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNAAQRMK
                     AIALETIAQEKRPGGSLNPNTQRN"
     gene            21022..21357
                     /locus_tag="ACHVB7_004738"
     CDS             21022..21357
                     /locus_tag="ACHVB7_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1916464.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERSGVESFYWVNPRGKQIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEA"
     gene            21440..22138
                     /locus_tag="ACHVB7_004739"
     CDS             21440..22138
                     /locus_tag="ACHVB7_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1916465.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            22131..22928
                     /locus_tag="ACHVB7_004740"
     CDS             22131..22928
                     /locus_tag="ACHVB7_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1916466.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLCRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDVFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            22916..23509
                     /locus_tag="ACHVB7_004741"
     CDS             22916..23509
                     /locus_tag="ACHVB7_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1916467.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAALLVVSAVMMPALAPLGLSLMMGGIAQIISPQATNESVRQADNSNSYYFD
                     GPQNTTNQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            23526..28232
                     /locus_tag="ACHVB7_004742"
     CDS             23526..28232
                     /locus_tag="ACHVB7_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1916468.1"
                     /translation="MEQFKKKKLPLLIAGSGGKKSSKSSSRTPVEADDTVNSRAMASI
                     LDLLGEGVIGGLVNGARSIFVDDLPIVNEDGSSNFSGISWDFRDGSQDQTPMSGFDFV
                     ETPKSINIQLKKSHYVTVSIDNDEADRVRVIMKFPSLRRIDQKTGDTNGTTVEYKFQI
                     SNGDSTFVDVVAEGEKNVGIKLTAKKTGAYYRSYELKLPKPGRAYSVRVVRITDDNSG
                     QYLYNDTWVDSIGEIVDTPMNYPNSALVGLKVNSEQFGGSMPSRSYLVRGLKIRVPSN
                     YNEASNTYDGVWDGSFKLLSSSNPAWILFDLLTNSRYGLGQYVSESMIDLGQLYQIGR
                     YCDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSP
                     SDPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRY
                     GERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNK
                     YRAGKRNSGRIVEFTKNSITLDAPVSLAKSGSFIRILNQEGKIVERDVLETGENITKV
                     TFSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETSGSFDITAVENNPSKYEA
                     IDNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISW
                     RRSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISTVYQVLGT
                     MTPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTF
                     THSGLGDSETWYYWVRSANKRGMLSRPNSNLGTEATTRDVLSFLANKITSSELGQALN
                     EEIDSKASQQAVDEINNHIDQSVESLEGEISALNKELTSSIDEFKTSFTERSETLENA
                     QSELRTEVSSTREKVDDALRQVENSNAALIELRNTVSKQGDSVAGVVEAANAALENAS
                     SLIAEERKVRAQADLANAKKIETMRSSVDESVAAVEEMRRTVAEVGRVSSEITTNLEA
                     ISKTNIDLALRQDEDQHRQLVNNAKIATTQKILVDDVSAMASKVEEIRAEIGDNIRAS
                     ILEEATARTDGDNALANRVTQLQSKFEGDISAAIRTEQSARASANETLSKQITQLESK
                     VNTDISTAIREEQQTRATQDEALSRQITQLQTKVNNDISAAIKSEQQARVSGDSALST
                     QINQLQAKVDDDISAAINEEREARASGDSALSREVSSLKTKTGKDIAAAVAVETKART
                     DANDALSRQISSLTTRANNLQSSIERESTSRTSQDSAIAKDLASFKTKTAKDISAAVA
                     VETQARAAADTALTSQINSLKTQTGKDIKAAVAEETKARTDADAALSTRVTNLQSQTT
                     NYINAAITSESKARTSADNALGKRIDTLKASVDGNVAVIQQQASAIADANRKVSTSYT
                     LKMETSTSGGQKYVAGIALGIDTTGLSQFLVRADRFGLVNNVNGKITTPFVIENSVAY
                     MNGAYIKDGTITNAKVGDLQSTNFVRGRAGWRFGKNGTLEINGNSGGNGRLVINGQRI
                     DVYDDNNVLRVRIGRL"
     gene            28327..31698
                     /locus_tag="ACHVB7_004743"
     CDS             28327..31698
                     /locus_tag="ACHVB7_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1916469.1"
                     /translation="MWYREGTITFTQGSNTLVGAGTAWNVTANGVLPGMIVVAPDNKL
                     YEIKSVTNDTTLTLVENYTGETQSDIPCRIITTYEGDLTQFSARFTALLSRMSGDAKT
                     VRNWLTAVDEVTLEREDGTNVNVKPLSKIIAEHNKHVEWYENNTNAINSAGDKAKQAA
                     ASASAAAKSANLASSKATQASNSALDATASKNAANKSASSAKASETSALEAKNAAAIS
                     ASTASTKATEATSSANAALVSKEAAKLSEMNAASGAGSASTSANQARESARSAKTSAN
                     QASTYASAARASKDEASRKSAEASTSASNASSKSAESAAKAEEAKQSQTSALASAERA
                     EIAATRAERALSAISLHDASLTMKGIVKLSSETSSSSEEFAATPKAVKAAFDLAKSKY
                     TANDATTSQKGLVQLSSDTNNASESLAATTKAVKSVKDLADRKAAINSPAFTGTPTAP
                     TATQGTNNTQIATTAYVREAISGLVGSSPEALDTLNELARALGDDPDFATTMTNKLAT
                     KQPLDATLTALAGLTTAANKLPYFTGNDTAGLATITSVGRDILSKASVQAIIQYLGLK
                     ENGTSGDKIPLLSTANTWSAKQTFSNGLAGSLNGNSDTATKLKAARKIGGVPFDGSSD
                     INLPGVNSTGTQSTSGNAASATKLATPRSIGGMLFDGTANIDLPGVNKVGNQSTTGNA
                     GSATRLQTARTIGGVSFNGTANIDLPGVNKAGNQNTSGNAATATKLATARKINGVSFD
                     GSKNISITPKQLGAAKYVANLATGNTQEWTTAQFVAWLNSQGAFDATQWSTRCSWSYG
                     SNCYIGNASTGCGVIPLAGSVIEVFSTGTSNYTIRITTPSTSGGASGAVLNSEFIYTY
                     NGDNYSPTWRRNYSSQNKPTASEIGALASNGNAVSATKLKTARNIGGVSFDGTANIDL
                     PGVNKAGNQNTSGNAATATKLKTSRTIGGVSFDGSANINLPGVNTSGNQNTSGNAASA
                     TKLQTARTINGVSFDGTKNISISAAQIGALPIGGGTITGNLTVNGNGSFNDVQIRSDK
                     RNKRNLVKLDNALDRLEALTGYLYEIQLSDDSWKTSVGLIAQDAQKALSELVTEDTDV
                     ISGEKRLCLNYNGIIALLVEGFKTLRHEIKELREK"
     gene            31784..32296
                     /locus_tag="ACHVB7_004744"
     CDS             31784..32296
                     /locus_tag="ACHVB7_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916470.1"
                     /translation="MGVASGWVGSSAKSETGEQWMSAAGTKLGLGKPFMMSQMVGRSM
                     GCKIATAYYKGNPPDKVENWGAVGSDWPLAEKNQGTIAGASSCGLGRLVGIVLTQAHY
                     VPGLPPTGGLYIAGGRASNITVTVGGVSQVFTYNSVINGFHYYWQGTPSSAFITAMKR
                     TGVTQDLKIS"
     gene            32394..32717
                     /locus_tag="ACHVB7_004745"
     CDS             32394..32717
                     /locus_tag="ACHVB7_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916471.1"
                     /translation="MANDVGGVTHDQVERIAKIVAREVVGKLGKEIREEIVHEVHEQL
                     KNYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIISKVTSFIITALILGLAAYGV
                     KNGLQ"
     gene            32731..33423
                     /locus_tag="ACHVB7_004746"
     CDS             32731..33423
                     /locus_tag="ACHVB7_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916472.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGSSIDTIREVIERWAPPNENNTDTYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRKHQKGMA"
     gene            33425..33676
                     /locus_tag="ACHVB7_004747"
     CDS             33425..33676
                     /locus_tag="ACHVB7_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916473.1"
                     /translation="MLGSLMIKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQSTVDVKNETLDELRRKDASTVHHELHNKWLRD"
     gene            34002..34400
                     /locus_tag="ACHVB7_004748"
     CDS             34002..34400
                     /locus_tag="ACHVB7_004748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916474.1"
                     /translation="MHTALNASRSIQTIRDCTKMIASVPLRHWNDSLQIREMNKPGML
                     KDQRFIVNLKSDVEEGKFTIKLFAEMMVLNNRLRFLVKPEKQFPDVVRCEQDAFYEIE
                     SKIAKFIKDKYRVDVKIAKLDSSTDMRLAN"
     gene            34401..35177
                     /locus_tag="ACHVB7_004749"
     CDS             34401..35177
                     /locus_tag="ACHVB7_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916475.1"
                     /translation="MSPSFIAEYSKVLSDFPECEAREKIRVGYMVNSDFSVLRLTEWS
                     LIGSNYDYCLTVSFVGDPDADWLQEHAMDLDEQGHSEEEIVHSVRELVGDEQEDDDGI
                     QIARFVYKRFQFRSQKGSHLGVQIKGAFVTPSKEQKGLARRVYNFLLNWHDHVVCDDH
                     QTVYGARIWAVGMLLVGRVQIYDNLNKEFIDVLVEGGIGQNGVKPWDALLLNSEAQLR
                     HWNPMAVSIDPSSQILTIISKEDRHIEVGVTTFDARSTNH"
     gene            35340..36008
                     /locus_tag="ACHVB7_004750"
     CDS             35340..36008
                     /locus_tag="ACHVB7_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1916476.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            36014..36367
                     /locus_tag="ACHVB7_004751"
     CDS             36014..36367
                     /locus_tag="ACHVB7_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916477.1"
                     /translation="MKMGDLAKRKESDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            complement(36428..37486)
                     /locus_tag="ACHVB7_004752"
     CDS             complement(36428..37486)
                     /locus_tag="ACHVB7_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916478.1"
                     /translation="MRMNRLPGYGLPELAFWPQPKYERNKWSMFCLKLRNDGTLAWYR
                     RYVDRGMPNHAVDDDYDNYPEARKAALELNKNVSFDIEKLPLSMFQKKSLRLKVDKAL
                     KAKSRLMDEEYIMLNEAIKAHANDPRVTLNELIINPDYETLRQPLFDVLSEMPYLHFV
                     FLPKFQVSLQLVAPNTWECSYNNKAKTAKICYQERIARGFGLSGAAHWGKTKATIRSM
                     LLPRANRLLQLASVKRMLDEALRNGQKVLVVGNFVFWFEDKNQVGWSVKVASESETTS
                     RGNTLWKEGTIISKNHGRIVVLPYTKENGKHVKGYTKNAPNDGNAIPRHKDEYVELPF
                     EILDGDLMFSLFGELNYE"
     gene            37776..38516
                     /locus_tag="ACHVB7_004753"
     CDS             37776..38516
                     /locus_tag="ACHVB7_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916479.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FETKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELVEIFEEK
                     LARRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILECLRTIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARRFIDPLSN"
     gene            38560..39900
                     /locus_tag="ACHVB7_004754"
     CDS             38560..39900
                     /locus_tag="ACHVB7_004754"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFR1916480.1"
                     /translation="MTELCHTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDLVVPS
                     QFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFADVVAAFKRI
                     LAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIGSNEA
                     TGIYDYYTSASERLKAREYEASEEYVPNSITTGLPLLDRLLYQKGWAKREMVLFMGFA
                     KSGKSTAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDARLSETEMSKLVERRDEVH
                     RKLAELGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMVVVDYADLMRAS
                     YDLRDDRANIRSIYTDLRALYDKHNVAGITASQTNREGGASEVATMMHAADNIEKVRI
                     ADLVITINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT"
     gene            40048..41163
                     /locus_tag="ACHVB7_004755"
     CDS             40048..41163
                     /locus_tag="ACHVB7_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1916481.1"
                     /translation="MSELKELIAELDFEQWLDTEGIVYRRGGVSTRGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEELVLASTVELEGPVALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLTSIGLNVKVACLGEEGL
                     DPGGATPEQIIKAYYRAKPYTKQLELQSKVLGIKALS"
     gene            41239..42015
                     /locus_tag="ACHVB7_004756"
     CDS             41239..42015
                     /locus_tag="ACHVB7_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916482.1"
                     /translation="MKDEIQKLACDIIDKTGLEISESNRLDIIEKAVKTAMDHIATRL
                     VEIPLPGLPYLKVKLHVWGEPSCARRSALVVFISKENPLSLKVQVGAWLDGRVIYTNT
                     VFCLSNDETIEAAIQEAVLAMRSSGLMKNNYEEYLRSISGEKTLSLKADFVTPKNLLE
                     VLLNKGANDAVNVIRESEYASLCDMCKSQLDLVHIVIDAGKACDGVMAEFAWKVVRIA
                     NELPMIEQEAKSYATNHVTELLAPYRLESNQRKMISWGSW"
     gene            42071..42553
                     /locus_tag="ACHVB7_004757"
     CDS             42071..42553
                     /locus_tag="ACHVB7_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916483.1"
                     /translation="MAVVVKADLSKIPSISGNNGYSLRCEEVKINGESAYCSYSVCQH
                     TILAFKENRLPRTSFQSCATAIKAGKCKALKMMVEEIRKGESLYFEDMTALIKEVEER
                     NKQARTLKRKRDSVTINSMVKKSTTSQTAITDVYAALLEETTKETHEQIDQHMEVKQQ
                     "
     gene            42550..43872
                     /locus_tag="ACHVB7_004758"
     CDS             42550..43872
                     /locus_tag="ACHVB7_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFR1916484.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESIPSDEKAIDVLNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTGMKLKERLAML
                     VALFKNDRSEGYTQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            43869..44084
                     /locus_tag="ACHVB7_004759"
     CDS             43869..44084
                     /locus_tag="ACHVB7_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916485.1"
                     /translation="MIVLSKREKETLHEISKWPEFPQYWKPKTRANLERLGLVANISE
                     TWCSANYQLTDKGKVLLQQLVESGVLK"
     gene            44230..44520
                     /locus_tag="ACHVB7_004760"
     CDS             44230..44520
                     /locus_tag="ACHVB7_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HFR1916486.1"
                     /translation="MSNLTSWDWWLATYFLAVGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            45280..45354
                     /locus_tag="ACHVB7_004761"
     tRNA            45280..45354
                     /locus_tag="ACHVB7_004761"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45312..45314,aa:Asn,seq:gtt)
     gene            45360..45446
                     /locus_tag="ACHVB7_004762"
     tRNA            45360..45446
                     /locus_tag="ACHVB7_004762"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45394..45396,aa:Tyr,seq:gta)
     gene            45451..45526
                     /locus_tag="ACHVB7_004763"
     tRNA            45451..45526
                     /locus_tag="ACHVB7_004763"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45484..45486,aa:Thr,seq:tgt)
     gene            46017..46103
                     /locus_tag="ACHVB7_004764"
     tRNA            46017..46103
                     /locus_tag="ACHVB7_004764"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46049..46051,aa:Ser,seq:tga)
     gene            46329..48242
                     /locus_tag="ACHVB7_004765"
     CDS             46329..48242
                     /locus_tag="ACHVB7_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR1916487.1"
                     /translation="MSFIDYDILSASSSKELREKLAEKIAEGWQPFESPNIVEPSKGG
                     PNAYNQAIVRSGDLSQLPTPVSNEDEEPDYYYVVTIAGQSNSMSYGEGLPMPKTFDRP
                     HPRVKQLARRSNIKPGGFACKYNEIIPADHCLHDVQDMTKFKHPKANDSEYGCVGQGL
                     HIGKKLLPFIPANAGILLVPCGRGGSGFTVTGSEGQYYEESGAIESSLKWGLNTPLYK
                     DIVARTKAALSKNKKNVFLGVVWMQGEADLATGKYSQHNELFLKMVEGFRKELSQYDA
                     QCVFGSAANVPWICGDTTHWWKNKYPKQYEIVYGGYKGKTSQAIYFVPFMKDENDNNV
                     LTNSPTDDPDITSIGYYGSGHRKDNKTWTSESRESHFGSWQRRGIISDRLATALLAHS
                     AKSSAFVNGVTGEIIKQEQPTQPKNDNIVTTPTPRTVISLTGAGGDVATEQGWTVTGA
                     NIQSVENGKAIALTKAVSNETWTISKATDIAQALLENGGKLLCKFKLSGNPDPKKAKF
                     GFGIYYKTSKPVADDISFDGTGSLFLANFFLQTIGGKLNIMMHRTGNNRNVQIDSYGD
                     YDNNWHTLELQYEKGTGTVIPVLDGKAGSKFKLVKDNIALGDEAFVLTDITNNQTHDL
                     AIETLTISINNPT"
     gene            48450..49553
                     /locus_tag="ACHVB7_004766"
     CDS             48450..49553
                     /locus_tag="ACHVB7_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1916488.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAIMISQINNNMIFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSTLMRTCIEELFEYGKDSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELA
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRLNFS"
     gene            complement(49548..49934)
                     /locus_tag="ACHVB7_004767"
     CDS             complement(49548..49934)
                     /locus_tag="ACHVB7_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR1916489.1"
                     /translation="MSDKNIEFIESRYAAFIAGLIESSPMSQAQIARLIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
     gene            50182..50394
                     /locus_tag="ACHVB7_004768"
     CDS             50182..50394
                     /locus_tag="ACHVB7_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916490.1"
                     /translation="MKTTISSLITLEIGHVEKLADECVADILTELPNEQIQVGMHDAT
                     AFIFLLNNKRFTLINTGSGSLAVRTC"
     gene            50493..52601
                     /locus_tag="ACHVB7_004769"
     CDS             50493..52601
                     /locus_tag="ACHVB7_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HFR1916491.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSRSLG
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDADQTDASGENNEDNEDHETPSALDNHKSIESES
                     HSKHKHDNNDSDDSDNSESSETIFDDTENDKEVSDSDASDNAASESLTADHEKRKTTE
                     DGSSDIPTPSKMSLEEALEELDSIEDEVGGMTEDALSETIKSELTESSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLAMNDDRVFRKKEESKAVNACVQQ
                     VIDLSGSMSGITIQLALASSYTIADALDRINVPNIITGFTTFGSHMAAGELKAVKYEF
                     SRFESLMLPIIKNWNEKVSSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRMED
                     RKIMLVLSDGAPWAVGRGFDAHLRSVAKQIETQTDIDLMAIGIMTDAPERFYSNHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            52697..53932
                     /locus_tag="ACHVB7_004770"
     CDS             52697..53932
                     /locus_tag="ACHVB7_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1916492.1"
                     /translation="MTTTALQNEKNPSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGLTSSPGYTGTVE
                     YKTDLVAAHELLNVTVEELGTKRGTPLMVSVNVNTPYPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKDAEINMLTNA
                     GIVDEYAEKMVKFAGIVRDGYEEHNISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            54113..57631
                     /gene="dnaE"
                     /locus_tag="ACHVB7_004771"
     CDS             54113..57631
                     /gene="dnaE"
                     /locus_tag="ACHVB7_004771"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1916493.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRDKLAKESKGCMESLERGRNYSFTALIK
                     NEQGYRDICELMTVANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITPGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAVNGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDTIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELKNVEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDTISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPESLHPVLD
                     ELTAETNGVILYQEQTMNATRLLAGFTMAEADGVRKAIGKKDMEKMKSMGEKFIVQAQ
                     AGWIDVELEDGTTQRIHRAEHFKCEDGTMKTVEEALEYGAKLPINAVRVTASHPGLSE
                     MKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQVLYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESLAQFEEAVEKRACNSRVRESLQKVGAFASIEPGSLPATDPERLRDQAE
                     LMGNLVIDAVKASRPFEMNSKRSAEINVLMTRMAAEMGLGDELIRPSIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTAGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFMREEINLVRPTYVLTCGSRATALFNNKNKPSDLVGRKEYLPDLDVTVFYGFNP
                     NILYFRPEEGERLEAILADVAETIKS"
     gene            57757..58188
                     /locus_tag="ACHVB7_004772"
     CDS             57757..58188
                     /locus_tag="ACHVB7_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916494.1"
                     /translation="MITDIYEKIMSDLEFDRDNLEEVWRRQPRLLMEYGSKLAHAERS
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDDARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            58306..59334
                     /locus_tag="ACHVB7_004773"
     CDS             58306..59334
                     /locus_tag="ACHVB7_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFR1916495.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVAAASATEDEGLDD
                     LLRELDSL"
     gene            59395..60339
                     /locus_tag="ACHVB7_004774"
     CDS             59395..60339
                     /locus_tag="ACHVB7_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HFR1916496.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVASIAPQPIVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDKSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVDKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            60339..60605
                     /locus_tag="ACHVB7_004775"
     CDS             60339..60605
                     /locus_tag="ACHVB7_004775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916497.1"
                     /translation="MLKSIINGATTTPTQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            60608..61681
                     /locus_tag="ACHVB7_004776"
     CDS             60608..61681
                     /locus_tag="ACHVB7_004776"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1916498.1"
                     /translation="MAKGKSALALALKKKIGSNDEIQKVTHWIDTGFPPLNKAISGRY
                     DGGFPCGRIVEVFGPPSAGKTFLATAAMISAQKQDGLAVFLDHENSFDVGLAVANGLN
                     ADEDDGQWVYKQPDTFEDSVELIGTILKLVRDEELIPESAPICIVADSLASMVPNSKA
                     EKFEKMAEGTAKDKDQLNMNDNTALARATSANFPTLALWARKYNACIIFLNQVRTKIG
                     VMFGDPTTSPGGDSPKFYASVRIRLGASVMKDGKEKIGQDVGAECIKNKVAPPYGKCT
                     WKFYFDPTRGLDVIESLVEYMLEEGYLPKNASGRVEIGDKKYTKSQIVEMYREKPLAE
                     IIAALQAIDDRRAKDNPTESVEE"
     gene            61774..61974
                     /locus_tag="ACHVB7_004777"
     CDS             61774..61974
                     /locus_tag="ACHVB7_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916499.1"
                     /translation="MIKIYLLAVATGVSVALIYGLLVPSLISTKSDLAVMFGVIVGFG
                     APVIGLIAGRKFINSLIKAKGK"
     gene            61978..62808
                     /locus_tag="ACHVB7_004778"
     CDS             61978..62808
                     /locus_tag="ACHVB7_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1916500.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            62904..63728
                     /locus_tag="ACHVB7_004779"
     CDS             62904..63728
                     /locus_tag="ACHVB7_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916501.1"
                     /translation="MPNHITNEIRVIGGTNKQRLDFIRAITNKKGLIDFNTICRRPKS
                     IEMEENNHIRRMASAMAGETVYDHMFGELKTPEQVELAMRESGMTRKEIRKIKEQALM
                     RLENYRRYGYYSWFDWSRANWGTKWNAYNIEMPVKRIPTRIKYGHNFRHTHVRAYAKR
                     IYKKRLARHAATGGELVIRFETAWSMPKPIYQAMARKFPHLEFVIRYADEDFGNNCGF
                     ILLRNGKWEYDYIAPANNKQSSEEKIKWRKFAFELCCPGITPQEYGLNEEYEYAGY"
     gene            63776..64516
                     /locus_tag="ACHVB7_004780"
     CDS             63776..64516
                     /locus_tag="ACHVB7_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916502.1"
                     /translation="MKRKIEEPKTTLNKHGEKIQTHPAFGLVKTSRVSTTGIRLFDSE
                     LEHRDYIEIGVYEAELSLDNERTRPQRSCNRRPLVEIRLSQAQWAAMVSSFGVGEGVP
                     CTISYKTIGDSIRVHDIAEQKSIRQKFNDQIKAATNSEIDRLNQQVSELGELVKKGRA
                     GKRDLEELYRKLASAVANLPENLCFATELMQEQMDEIVSSGKAEIEAYVAGTVMRKGL
                     AALGTESCINDDPTVSITKMESGKELLP"
     gene            64537..64926
                     /locus_tag="ACHVB7_004781"
     CDS             64537..64926
                     /locus_tag="ACHVB7_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916503.1"
                     /translation="MLLIQPGFGLKIKKGHMFGERDSKRKILSIRLPFITIHWLNRES
                     TDYWYKCARAAFNDPDWFVENHHAVRQAKRKANTTYMKAYQDAWEEHRGRYQKNIEKL
                     ESENMDLRRKLGEARRDIEAYKRLVSE"
     gene            64988..65413
                     /locus_tag="ACHVB7_004782"
     CDS             64988..65413
                     /locus_tag="ACHVB7_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916504.1"
                     /translation="MAAIKKLYDAANVALDVIDDEVAKGFPEPDWAHQLRNAIAEMTP
                     PDPTPDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPNQED"
     gene            65469..65744
                     /locus_tag="ACHVB7_004783"
     CDS             65469..65744
                     /locus_tag="ACHVB7_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916505.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKVDGLNDEETDALIQRTLVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISNEDAEHLERILSISTFE"
     gene            65960..66295
                     /locus_tag="ACHVB7_004784"
     CDS             65960..66295
                     /locus_tag="ACHVB7_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916506.1"
                     /translation="MKTIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            66295..66507
                     /locus_tag="ACHVB7_004785"
     CDS             66295..66507
                     /locus_tag="ACHVB7_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916507.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(67087..68301)
                     /locus_tag="ACHVB7_004786"
     CDS             complement(67087..68301)
                     /locus_tag="ACHVB7_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFR1916508.1"
                     /translation="MLSAVIDSLSAVHRMLPTVFGILSAANQVCCQRLNDKGVHMSTK
                     NKKGKNNKEVEDNIDNFEEGSLELYTGELVPNSNNTVQPIALMRLGLFVPTLKGTKYS
                     KRNKPNEIDASKELVQLEVARSEGYSDIKITGPRLDMDHDFKTWVGVVRSLAEYGEPN
                     GRVELSITKFAKFCGYPSSQIRKTLRDRLTNSLLKIMRTTLSFQRTYEEKNVDGSNKI
                     SLLMVHLINSVDYNEQKDTVVFYAEPKLAELYRFDHKVLLQLKVINKLPRKETAQALY
                     TFIESLPTKPAPVSLARLRARLNLSSRNVSSQNQTIRNGLKALQDLGYLEYSEIKRGR
                     SIYIQIHSRNPKLKVAPPKPEDIEPKKPDEKAGEIDAKQNIINKITELSQNLTPENIK
                     MIEILSNSLKLL"
     gene            68503..69147
                     /locus_tag="ACHVB7_004787"
     CDS             68503..69147
                     /locus_tag="ACHVB7_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916509.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            69402..70487
                     /locus_tag="ACHVB7_004788"
     CDS             69402..70487
                     /locus_tag="ACHVB7_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFR1916510.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            70717..72633
                     /locus_tag="ACHVB7_004789"
     CDS             70717..72633
                     /locus_tag="ACHVB7_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1916511.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEIVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            72623..73366
                     /locus_tag="ACHVB7_004790"
     CDS             72623..73366
                     /locus_tag="ACHVB7_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916512.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHENEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NSPQPTLQTAIVTNSLEARLLENPIWGTW"
     gene            73376..73945
                     /locus_tag="ACHVB7_004791"
     CDS             73376..73945
                     /locus_tag="ACHVB7_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916513.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHDLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHSKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            74019..76334
                     /gene="nrdA"
                     /locus_tag="ACHVB7_004792"
     CDS             74019..76334
                     /gene="nrdA"
                     /locus_tag="ACHVB7_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1916514.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGMTTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQELFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGIRYSDTAGNKLVHETFEAIQYYLLDASCQLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEIEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            76440..77582
                     /gene="nrdB"
                     /locus_tag="ACHVB7_004793"
     CDS             76440..77582
                     /gene="nrdB"
                     /locus_tag="ACHVB7_004793"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1916515.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWAEYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            77660..78529
                     /locus_tag="ACHVB7_004794"
     CDS             77660..78529
                     /locus_tag="ACHVB7_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFR1916516.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            78697..79800
                     /locus_tag="ACHVB7_004795"
     CDS             78697..79800
                     /locus_tag="ACHVB7_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1916517.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQTDDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRFGYESIYTKMLYDLEMDGSVTETELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDDILILGYDPLPWIKVPVAV"
     gene            79802..80215
                     /locus_tag="ACHVB7_004796"
     CDS             79802..80215
                     /locus_tag="ACHVB7_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916518.1"
                     /translation="MARGMYVLCEIEGVLANTSHRKSVPDTDAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            80212..80688
                     /locus_tag="ACHVB7_004797"
     CDS             80212..80688
                     /locus_tag="ACHVB7_004797"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR1916519.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            80688..81332
                     /locus_tag="ACHVB7_004798"
     CDS             80688..81332
                     /locus_tag="ACHVB7_004798"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1916520.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            81394..81813
                     /locus_tag="ACHVB7_004799"
     CDS             81394..81813
                     /locus_tag="ACHVB7_004799"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916521.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            81823..82380
                     /locus_tag="ACHVB7_004800"
     CDS             81823..82380
                     /locus_tag="ACHVB7_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFR1916522.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMEACSAWVAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            82541..83350
                     /locus_tag="ACHVB7_004801"
     CDS             82541..83350
                     /locus_tag="ACHVB7_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916523.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESGEAAENEPEQL
                     GIIEVEDCVEVNDELKVQQSNDSNTNKKTRAARGPSFTLSDKDDSFFNKAGLEKDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            83533..84126
                     /locus_tag="ACHVB7_004802"
     CDS             83533..84126
                     /locus_tag="ACHVB7_004802"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFR1916524.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGIERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATEYANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
     gene            84314..84544
                     /locus_tag="ACHVB7_004803"
     CDS             84314..84544
                     /locus_tag="ACHVB7_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916525.1"
                     /translation="MSYPTNVVALVDSDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSK"
     gene            85126..85719
                     /gene="rnhA"
                     /locus_tag="ACHVB7_004804"
     CDS             85126..85719
                     /gene="rnhA"
                     /locus_tag="ACHVB7_004804"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1916526.1"
                     /translation="MTTKTPARPQAKTRKKDKNKNSPRTNPTTPVVEFNPQLKTVKIF
                     SDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRMEMMGALIGLERLKYPCNV
                     ILHSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWKRLDEAASRHNVRWKWVKG
                     HAGHRENEICDRLAKIAAFSAADMPHKKDIGFVYNKQ"
BASE COUNT        24759 a        18295 c        20817 g        21946 t
ORIGIN      
        1 ggggtagaat tgaaagagta taaaatgaat ggcatgtaaa ataataaccg gacttagccg
       61 gttattaccc ttatagaaat taaaacggaa ggattcgttc aacccaatcg aagaaaataa
      121 gtatttttct accgtcttcc aacttaagtg tgactagaca ggcgtcaacg ccatctgacg
      181 ttccctcaat ttcgcgaccg tctgccatgt agacccttat agatttgccg ttttggtaag
      241 cctgacgaca tatcttaaaa aagtcacgac gtgatggctg attaaccatc tgttcattgt
      301 ttagcgttag tcgacctgtg aatgaagtgt caacctcttc tatgcccgac tcaattgtgc
      361 taatgcgctc aagagggagc cttatacgat ttgtgttgct aagcggatct ggtgtgaggt
      421 tcagcttatt tcttgcgctc aatagcccgt agacatacat gcaaaacact tggccgtctt
      481 cgagagtgac tcttacagga aattttgcac tccgccagaa gagcaaggta cgttcagcat
      541 gttcgtagtc tcgcggccat acttcagccg gtatgccgta ggtgatgtcc aatgttctca
      601 ttctgatact cttagaggtg ttttcagcaa cccaaattta aattctttgt taccaacgac
      661 aaagaaggga acctcaacat agattttttt atcgacggat ttagagttcg ctgttttctg
      721 tatgtcacga acgatacgtt ttgaggtatt ttcatcgata aacaatacct ttccacttgt
      781 attcccttca gccttggcgt aagtaacgat aggctcttca ttgccaaccc taatgtttac
      841 ttggcaacct gttgtagcca tttcatcaca gtaaagtgtg ccgtcttgaa cttccaaaac
      901 aattccagat ggcttcatgg cttcgtcaga gcttacatta acgttttctg gtacagcggt
      961 tccgttaatt tgcaaccgta atctttcttt tcctgcatta agtattgttt cttctgcgtt
     1021 atctgagaat ctgaaccaga ctggttgaat cagacctcgt gattcgtatt ttagtacgtc
     1081 ttcagaagta tacatgctgt acgttttttg catgtcggag acgatatctg acaacgcatt
     1141 ggcggaaaat gacgagaggt atatacaagc aagaagtaac gaacgtttca tttttataca
     1201 gcgattattg caaagtagaa ttataaatat ccaatagtct caaacgataa gttttcaact
     1261 gtaaatagca gacgtgtttt ctctaaaagc tgtgcggcaa aggcgttgtt gccgcactct
     1321 gtctattagc tcttcaagtc tggcaattct gaaattgact aagaatccaa tattacggca
     1381 gtttgcttag aatttgctct aaatcttcct ttgtcatatt agagttctca tagatgcgca
     1441 tgattttctc acgagcctta gcagagactc caacggcaga tgaaactttg tcaaattcag
     1501 ccatcgtcat cgtctccaga atggtattga taacttcagc cttagacatt ttgatgtttt
     1561 tttcttttag tttcatttga aactttccaa gtttgtcatt ggccttatcg gacaatgcca
     1621 cctgacaata agttgttttc ttttcgctca taactaatct cgtttcagaa ctccaaaatc
     1681 gaatgcgcca tcaataggca atacaccttc tgcaaagcca ggtgtggtgt cgatgatgtg
     1741 ttttcgctca taagagtgag acaacaggta tttgttgcta atgtcaatga aatcagtgat
     1801 aaaacacacg tttgcctgat tcttcttggc tcgtaagcca cgaccgacac gctgcctcat
     1861 ttcaacttct gctttcccac caccagcaag aatgaccgca ccaacgcttg gcacatcaac
     1921 accgacatcc agaatagtcg agcctattaa aacatctatt tctccagacg ctaaactgtt
     1981 cagctttgct tgccttgtcg cctggttaga ttccccatag atgaagtcaa ctctaaggcc
     2041 ggactctttc atcatttcca tcaggatttg cccgtggcgt ttaaggcgaa ccagagtcat
     2101 acaattgaga gaatgttgct tatagagcaa tgcttcgcgc acaatggcct cgttacgtcc
     2161 caaattatac acgatcccca actgataagc cttttggtag gcggtgctca taccaactct
     2221 aaaattgagg tgtttgttgg caagttcggc cttgattctg gcctcgtctg gcttgtaggc
     2281 aactttatga taaaggaagt acggctttgc cagaatgcct cgatcaatca gatacttttc
     2341 tgtgacttta atttcaattc gcccggccac cgccatcagg cgcatgttgg cttccgtcga
     2401 atccttcatg aacggcgtgg ctgtaagcgc cagacgatag tctgcgttca cacataatct
     2461 ggcgatgtca tagaaatttg agcctgaaga ctcatgcgcc tcttccagaa taagaagaga
     2521 gacacttgaa aggaagcgtt tcaccaactc ccgacgtttg aggtggtagc ttttcttatc
     2581 tggtgttgca tcgcgtggtg gttcttcgag gaaacttgca agagtttgaa ctgtagcgac
     2641 gttgatatgt cgtgatacct ggaactcacc cgagccaatg actccaacct tttggtcttt
     2701 taaccacggt tcgccatttt tggcgcggta gtcgatggat ctctggaagt tttcggccat
     2761 ttgaaacatc agaacagagc gggttgttaa aaataatgtc attcgaccga tacgtgcagc
     2821 tgctttgcag gcaacgttag atttcccgcc accagtagcg atctgcgcaa tcatcattcc
     2881 ctctcgaacc agtgtttcca cagtctgatc ctgatacgca taatcaggat tgtatgggaa
     2941 tgggttaacc gccggatttg gtttaccaag cgccggggct ttgtctttgc ggatatgaac
     3001 acatttgatg cccgccttgt tcaaattagc cgccacgggc ttggcaaagc cagcagggaa
     3061 cgagtttttg ctccagttga acattgtgct tgtgcccttc cagtcgccag cctcgacttc
     3121 gtagctcaac atttgctgca ccagttgctt taccttgtca tctgcgccag aaataagcgc
     3181 atttactgcg ttagatacaa tccgaacagt cataaacctc tttccttagt gccttttgta
     3241 tgttatttgg ctattataat aagtaagtga ttacttagtg gattgtagca ataaaatgga
     3301 cgtaaaaatc acgattttgc aggttgatgt tgccaacctt cgcccgaata cctggaacac
     3361 caattcggtt ggtgcgcaga atttcgaaaa actgaaaggt tctatcgaaa aattgggctt
     3421 ttttaagcca attttggctc gtgaacttga agatggattt tttgaaatcc tcggcggcga
     3481 acatcgctgg cgtgccgcta ttgagcaagg aatttcaacg gttccagtgc tttctgtggg
     3541 caaaattagc gatgtcgtag ctaaacagat gtcactggtg gacaacgagc gatacggtga
     3601 agacgaccaa atcgcattgc aacgcttcat tgaagaaatt cagtcagaac ttgactatca
     3661 actgtctgaa atcgccccgt atgacgacga aatctcgatg gttttagcaa aagaggcggc
     3721 aatcgacctt gaagcactgg aggcgttgtc tcgtggtagt gatgagcctg tcgataccga
     3781 caaacgagag aaaaccgaac gtgtgggggc ggaacatcag accatgcgct tcaaagtaac
     3841 tttcgatgcg tcagatcgtg ttgcagaaac cataaaaaac atcatcaaag agcaggctat
     3901 taacaccggt aatgaaatgg agaacgctgg tgaggctctg gtgtggctgg tcgactacta
     3961 caaggagcgt atgtaatgac caaaaagttt gaaatcgtat atcgcgaccc ggcagatctt
     4021 attccctatg agatgaatgc caaaaagcat gatgaacagc agatcagaga tctggccgca
     4081 gccattaaaa agcgcggatt tgaccagcca attacggtcg ataagaacga cgtaattatt
     4141 actggccacg gccgtcgtga ggctgcaatt tttgctggac ttgagcgtgt accggttatt
     4201 gttcgcgatg atctcagtga tgacgaggtt cgtgcgaagc gccttgaaga taaccgactt
     4261 gccagcattg attacgatgc aattaagcta cagaaagagc ttgagtcgct tgttctggac
     4321 gatatcgagg ttttcggctt cgaagagcgt gagttgaatg ttctcgttgg cagtatgaca
     4381 gaagaaatgg acaccgactc gctagttatc gatcttggcg aagaaactaa acgacagaag
     4441 gatgaacaca ccgagatcag tcgtgaagtt gcagcggaag aagtacgtgt tgtcgacgta
     4501 ttgggcttta aaacgcttcc tgctggctct gccattgttg ttggtgattt gcttgcccac
     4561 atggaagaaa tgacgggaga aagcggggta gacgcatttg tggcatatgc ggagaaaatc
     4621 tcttccgggg agatggctgc atgagcaaat acatcatcaa cgtatcgttt cagacacgcg
     4681 taaataaaac cacgcgcacg ttggaaatcg ctgagtcgtt cgggcttggc ctggacgaaa
     4741 aagagtggac gctttacgac aatctggagc tggaagtgaa gcagggcgat gtggtgtaca
     4801 tcaccggcca atccggttcc ggcaaatccg ttgtgctgcg cgagttgcaa cgacagatga
     4861 aggatgaagg gctgtctgta gcctccatcg atgattttac cttcgataat gaggttaacg
     4921 tcatcgatca gttgggcaaa acgaccagtg atgcgcttgg gttgttatct atggctggtc
     4981 tgaacgatgc atatctgttt gttcgcaagc cttctgaaat gtcagacggc cagaaatatc
     5041 gtctcaagat tgccaaactg attgagtcag gcgctaaagt atgggctgct gacgagttcg
     5101 gcgctgttct agaccgtgta accgctcagg ttgtggcgtc taacctccag cgtgccgctc
     5161 gaaaggttgg tgcgacggta atggtggcga cgactcacga agacctgaag aacgcgctgc
     5221 gcccggatat gcagatcacc aagcactaca aagaacgcgt gaaggtggaa tatgcctgat
     5281 ttgaagatcg tagagctgaa gccatcgaaa gagactgata acaacaacgt ggaagtcatc
     5341 cgcctgctgg aagaagcact tcagcacgcc agagaaggaa aaagccagag cctggcgctg
     5401 ttgatgatca acaacgacgg cagtgttctt gattgctggc ataacggcgg gcgcccgtat
     5461 gtcatggtag gggcgatgga gtctcttcgc ctggatttca tcaatgccaa tatcgagcgc
     5521 aggtgatcga catggcagat atcatcatca aacgctaccg ccctgaagag tttccgcgtc
     5581 atctggactt tctggagcga atgactgtca caaagggaac tgtagaggac tggcacgctc
     5641 ttaagtcgct tcactacaaa acagacggca aacctttcgc gccaacttac tatcgctgcg
     5701 aacttgatga ccgtctggtg ggcgtcgtgg ttatggctta cccgaaacta ctgttggcac
     5761 ctcgccaccg catgtttcct aagttgaaac caaccactaa taccaccgta gctaaccagt
     5821 actggggtcg gtacgtgaat aacaactttg cggtgatcag tcgctcagtt gtggatactc
     5881 agtatcgtgg cgtaggcgtc tcttatcgaa tgattaacct ggttagcagg atgcatgacc
     5941 ggccaatcat tgagatccag tcctcgatga gcaaatacaa tcccttcgcc atgaaagcag
     6001 ggtttaagtt catccgccct gagcgaccga agagctatga aagtgcactg cgtgtattcc
     6061 agcgccattt ccgttccgac cctggtgata acgaggcgat cgtcaaagag ttgttcgcaa
     6121 tgagcgagtc tcgtcgtcgc cgtgcactgc gtgatctggt ggcggactac cacaagaaca
     6181 gttccctggc aaaagctggg cggaatcgtg gcacgacgat tcaggacatt gccgacagtc
     6241 tggtggacga ggccagcatt gtgaagctgc tcaaggacat tcacaacctg agctttacgt
     6301 ctccgttgta tggtgtgtac cgaaaccctg actttggtcg tcgactgcct gacacgctgc
     6361 cactgctggc attcgacaaa caacctttgg ataaaccgtt agaaattgct ttaccggcat
     6421 aaggatttgc catgacgtta accgacaaac aaaaggacat catcaaaacg ctcaatctcg
     6481 gttatgagcg aggtcatcta cttgatctgg acgaattgct tgaagttttg ccgtacaaga
     6541 caaccaagca aagcatccag ttctcaattc gcgctctgat aaaaaagggg ctggtggaga
     6601 aaggacatac gcgccaacgc agtgacaatc gctatcaccg ccggactctt gggttaacca
     6661 ctttaggtcg agccaaagcg aagttactgg tgatgtaatc ggtctgggag cttatttaaa
     6721 gacctgcttc tgtatatata aataataagt aacttattaa atatatacag aagcaggctt
     6781 agtaagacat gcccagacct aattaaacac cccagaaaac aagttggtta gcatatgcag
     6841 taaacaagtt gttttagagc gcatggacgc gctctgtgtg ttttagaggg atctatgacg
     6901 gtcgaaaaag acgaggtaaa aactcgcctg acaccagcgg agtgggccga agctgaagcc
     6961 aaatggacgt caggcgaata tacactctca aagctggagg aagagtacgg cattcgtcgt
     7021 gaaacactct ccagacattt caaaaagcga ggattagaga aaggcgcgga ctctgttggg
     7081 aagatggttc gtgagtctct taaatctgac gcagagcttc gcgctaaagc ccgtgcggaa
     7141 aagatagaag aacgtcgtac acgttatgac ggctgggcgt atgcgttggg gcagatggtg
     7201 atggtcgaag tcactacggc caaacgtgag ggtaagcctt taggggcgat tgaggattct
     7261 ctcaagagct tacagagagc cagtaatact cttgcaaaat gctttgaagt ttcgtccaaa
     7321 gcattgggca tggatcatgc ggaaaatgac gaggaagaaa ttccgaacct ggtatttggt
     7381 gagcttacgc cttcccaggt ggcgaaatta cgtcaggaag acgacgagcc tgaaatcatc
     7441 gatgacgaat tgcttgagac gttggaagaa gaagctctaa gcgaatttga tgcgacagat
     7501 gatggaagtg aaggggagga cgaataatgg caatcccgtc ctcgctcagt cttgtgcaac
     7561 tgcattctgg acagatgaaa gtcttccagt ctccgcatcg atttaaagtt gtttgtgctg
     7621 gtcgacgctg gggaaaatcc cggttgtcga tctccactat tattcgtgcg gcggcaaagg
     7681 aaaaaaagca aagagtctgg tatgtcgctc ctacttacca gatggctcgc cagattttgt
     7741 gggacgatct acaggaagtt ctgcctcgta agtgggttag gaaaaagaac gacaccacga
     7801 tgacaatcgt gttgaagaac ggttcggaga tcgccctcaa aggtgctgat aagcctgaca
     7861 ctctgcgcgg cgtagcgttg cattttgtag tgcttgatga atttcaggat atgaaggctg
     7921 acacctggta caaggtgtta cgacctactc tttcatcgac acgcggcggt gcactgatca
     7981 ttggtacgcc aaaaggcttc tcggaatttc acaaactgtg gactataggc cagaacgtag
     8041 agctgcaaag aaagggacag tggaagagct ggcagtttgt aactgccgat tctccgtttg
     8101 tacctacggc ggaaattgaa gctgctaaga acgatatgga cccgaaatcg ttcgctcagg
     8161 agtacctggc gagctttgag aacatgtccg ggcgcgttta ctacccgttc gatcgtaacg
     8221 tgcatgtaaa accgcttcag ttcaaccctc ggttgcctat atgggtaggg caggacttca
     8281 acattgaccc gatgtcttca gtaattttgc aacctcagcc aaatggtgag ctatgggcaa
     8341 ttgatgaatt ggtgctcttt tcctctaaca cggcagaagt ttgtgatgag cttgagagac
     8401 gcttctggcg ctggaaatca caggtaacgg tatttccaga tccggcaggt gcttatcgcc
     8461 aacatgctcg tggggagtct gacgtagaca tattcaaaga gaagggattc ttacgtgtcg
     8521 attattcgaa aaagcacccg ccaattgcgg atcgtgttaa tgctgttaac cgaatgctga
     8581 tgtccgcatc tggagatatc cggctgtata tcgatccgaa gtgcaagcat ttgattgatt
     8641 cactggaaaa agtcatctac aagcctggaa cacgagatat ggataagaca ggtggcattg
     8701 agcatagtgc agacgcattg ggctatccag tacatcgtag gtatccagta aaaaatcgtg
     8761 ttattcttgg tggttctcga taggtaagta attatctaag gttattcaaa tggaattgaa
     8821 cgacaaacaa attaaggatc tggtggcgcg acgccacccg gaatacgaaa agaaaaaaga
     8881 acattgggac ttcctcgcca gcacttacgc tggcgggcgt ggttggttta cagacaatat
     8941 ctttcgttac tttaaagagg gagatcagga gtttaaggag cgagttgaac gtgcttatcg
     9001 cttcaaccac actcgtgagg tggtaaacct catcaacaaa tatctcttta aagaagacat
     9061 tcatagaaat atcgaagagg caccagagca gatccgcaat ttctggaaac gtgcgactcg
     9121 ccagaatgcc tctattgact catttatggc cgctattgat ttgcagtcgt ctatttacgg
     9181 tcgcatatgg gttgttgtcg atagcacgat gagtggtgat gttgagtcag tagctgacga
     9241 gaaaaagaaa gatgctcgcg cctacgccta ctggatttca cctcagcaaa tgctggatgt
     9301 ggcatgggac gacgacggga atatgttgtg ggcgttaatt gtggaagtcg ctcgtgatga
     9361 cgcagatcct tttacttcta caggtcagga ataccaacgt tatcgtctgt ggacacaaaa
     9421 cgagtggtat ctgttccgtg aggaagtgaa gaagggcgct ggtggagcag gtcgccgtca
     9481 ggcaaaagtt attttagagg atagcggtga gcataatctc ggcgtagttc ctgtgtttcc
     9541 tgttgattgt attggagaaa gtgaatcacc gtatttcagc ccatcgttga tcgatgatat
     9601 cgcttatctt gatcgtgcgg ttgcaaacta tctgtcaaac cttgatgcca ttattcagga
     9661 tcagacattt agccagttgg ctataccggt acagtcgctt ttacctggtg atgaaaacca
     9721 cactaaagtg cttgaaatgg gcacaaagcg agtcttcacc tacgattctg aaggtggaaa
     9781 ccagccgttt tatctgtcac cagacccgaa acaagctcag atgatcatca ctacgattaa
     9841 gacggtgatt aacgaaatct accattcagt tggtgtagct ggtgagcgaa cgaagcagga
     9901 caacgcacag ggaatcgata attcatctgg tgccgcaaaa atgtatgact tccagcgtgt
     9961 aaatagcttg cttgtcacaa aagcagagcg tctggaaagg gctgaacgcc aaatcatgct
    10021 actggttgcg aaatggatgg gggtagatct ggacgaagac cactctttaa ttgcgtatcc
    10081 agaaagtttc gatattcgtg gccttactga tgaattttct gttgccgaga aactgtcatt
    10141 acttcaggca ccggactctg tacgtcgtca ccagatggaa atgcttattg agaagatttt
    10201 cccgaacatt actgaggcga tgaaaaagga atttgataaa gatctcttga attttcctcc
    10261 aaaaaatgat ctaaataccc ttgaaaataa gtcagtactt acttatgatc gtggtgcagc
    10321 ccaagaaagc gggcaagatc aaccccgagg gaatggggac tcatctactc aagagaacga
    10381 gtgataagta acaaaaggaa tttttatgaa tctgtggcaa atgcttttgg cccgtcgtgg
    10441 tctgatggat gtcgctgaag cgcatgagcg tggaggcgct ggcggtgtag ctgctgataa
    10501 tgagcagagt acacaagatc ctgacaaaca gggtgaacaa aaagagcagc cgaagggcga
    10561 tgacgaatac gctggcatga ctcaggaaga gttactggcc gaacttcgta aaaccaagaa
    10621 agctggtgct gaactgctga aggagaacat gaagcgcaaa gagaaagagc gcacattggc
    10681 cgatcagctt gctcagtacg gtgatatcga cccggcgcgt gctcgccagc ttttagaggc
    10741 tgaacaggcc gcagaaaaca cacgtcgtga ggcggagcaa gctgaactgg agcgtcgtgg
    10801 tgagttcgat gctgttaaaa aacagatgat cgaagcacac caggcagagc tggcacagcg
    10861 tgacgaacgt tatgcagcac tggaaagtga aaacgcatca ctgaaatctc aattagttga
    10921 gatgaccgtt ggcgcgtcat ttagtaattc tgccttcctt cgtgacaaag ttctaatgac
    10981 tcccgcgaaa gctcgcgtga tctacggttc tcattttgaa gtcggtgatg acggtagcgt
    11041 agtgggttat gacaaaccgg caggccacaa agatcgagct gttctggttg acggtgaagg
    11101 aaacacgttg tcatttgaat ctgcgattga acgcattgtg cgggcagatc cagaggctga
    11161 cgcagtcatg cgcagcgaag ccaaacaggg tgctggttct aatagcaaac caacccataa
    11221 ggtagtccaa cagaagtcta agtcgacgat ggacaagttg acctctggtc ttgggaaaat
    11281 tggacttaag taacatctaa atcaaaggga attgatagat gccattactg cgtgaagaag
    11341 ctgaaaagct atccaataat gaacttgaac agggtgtgat cgaaactatc atcgatcgcg
    11401 atgacctgtt tgcgattctg ccttttatga agattaactc gaaggcatat ctttataatc
    11461 gcgaagcaac gcttagtgaa gcgaccttta ttgatgtaaa cgatactatt gcggaaggcg
    11521 cagcaacatt cagcgaacat gttgcaaaac tgcgtattct ggctggtgac gttgacgttg
    11581 ataaattctt agcaacaact atgtccgata ctaacaatca gttggctgtt caggttcgcc
    11641 agaaagttaa aggtctggct cgtgcattcc gtcgaaatct gatcttgggt aattctgaaa
    11701 ctaataccaa agcgtttgat ggtattccgc gtctgatgca caaagatcag aagatcgaca
    11761 ttgctggtgc atctatgact ttctctatgt tcgacgagct ggtcgatgcg gtgaaagatt
    11821 tgggcgctga ctgcatcatg atgcgttcag agcacctgcg tgcttaccgt gcgctgctgc
    11881 gtacagtaaa cgtaggcccg tctgaaatca tgatggagaa cttcggtcgt cctatgctgt
    11941 gccataatgg tattccattt attgttaacg acttcattcc taaaaatggg gttaaagtta
    12001 ctgaagattc agagcagaaa acggaaggcc aggaaggcac tgctaacatt tattgtctgc
    12061 atttgtcaga agaaaatggt cttaccggtc tgtatggcgg tgacaatgct ggtatcgttg
    12121 tggagaacat tggcacggta cagaataaag atgcagtacg tactcgtgtg aaatggtact
    12181 gctctctggc gaataagcac gataaggcta tcgctgcact gaacaatgta aaaatttaac
    12241 taaaataata ggtgtgtaat tacctatgct taagggtggg ctatacgccc accctttttg
    12301 taggttttaa atatgcctgt atagcgagaa agcgtaatga aaccagcaaa aattcgttta
    12361 ttggaaccgc aattttctga ttattcggga atgttgtgtg gagttaagtt tgagaatggg
    12421 gtatccgttt ccgagttacc ttttatcgac cagcagagga tttgtgcatc aatgcgcgca
    12481 tcaactgttg agggtaaaaa cgtttctcca tctgctgcat atggcgaacg taatgatctg
    12541 aacgtagatc agattgttga gccatcagca cctgacattg ttccaatgaa gcgcggaacg
    12601 gcagatgaac cagctaaacc aattcaaact ttcacacggg aagaactgga gtcaattgct
    12661 gatagtgagg gtattgccgg tttacgttta attgggaata agattggtgt taaggcgaaa
    12721 ggaattgtcg aaatgattga cggcatcatg aaagcacaag gcggtgagta atggcgcaga
    12781 tcgactcgta tcgtagcggt gaagctgttt ctctttcatt tgccttcaac gtgctggata
    12841 tcgaatctgc cacctacaca gtaaaggaca gcaccggggc tattctcgtt gatggcgagc
    12901 cgctagaaat taccagcggt caaatgtcga ttccggttgt cgtgtcggct gaatataacc
    12961 agctctctga gaaagaacga gatctgcggt acgtcattgt gaaggctgtt gcatcgggcc
    13021 ttacgcatga agagcgtcaa atgtatgttc tgctgaatac ttttgaactg tcgataccag
    13081 aacagtcgtt tgcaactgtc gctgatgctc aaatgcaggc gatcgatatg ctgaatgggg
    13141 acactttgct gtcggatggt gaaggcttaa tgcgcaagcg tctcattgag gctacaagac
    13201 gtattaaaac tttaccgttc tcaatccgca aaattctacg tatcgacttt gaccgatacg
    13261 atcgccctca aaatatgctg aatgtgtatg acattccgtg gggagcagat ggagcgtacc
    13321 ggcatgattt agtcgattgg gaaaagatga ctcaggagaa gtttgaagag ttccctgact
    13381 acttcaaaga agcgttaatg ctagccgttg ttaatgaagc gtgtgagata gcaaatggca
    13441 atgatatcgt cgcagcacga gaggatggca ttttgtcaga atcgatcggt gaaacgacca
    13501 acatgtatcg tacaggtaaa tcagcaaacg tgcatgttgc tcgcagcacc tggcgtttac
    13561 tgatccgtta tatagacaat cgtatgattg ttcgacgttc gtaatgtctt tctcattatt
    13621 tggttttgga agcaatagtt ttggctggca atgagatcga caacagggag agagcgtgaa
    13681 tatttcatgg caaacagaaa ttgcgatcta ccgctttggc acaaagaacg tctacggtga
    13741 agcgcaattg cagttcgtca ggaagacgaa tgtcggtgta gttaaattcg aacagagtaa
    13801 tgagaaatcg tcagttcgtg ctgatagctc tggtagtcgt ggcaaggcga gtctggagct
    13861 gtttgatgct gtgttagttg tccctttgga atcggctgta caacttgatg atgtactgat
    13921 ccttgaaggt caaaaattaa aggtgtccag tgttcatcgc cgctgggggc tacgaggaag
    13981 acctgggcat cttgaagtag gggcgaatat atgggtctga aatacgatgc gcatcagttc
    14041 aaacgtgcgg ggaatagact caacaacagc cagaaagcat ttaagcgata tcttattcgg
    14101 gatatggaaa agctggcgcg tttggttgag cgcctcagcc gtgcaatggc cccgctggaa
    14161 accggatctc tggagacagc catctttgct agggtgatta aagaaggtta ttcagggctg
    14221 cgcattgagc tttctgtgtc tggagcaaag ccacgtcagg ggcatcctgg cgtagaagta
    14281 ggtgactatg ctaagtacat ggagctgggc aaataccgtc tcggttatct ttctcgaatg
    14341 aagaacgtga caaacccacc aattgaaggt gtaaaacctc gtgttggtcc acactttctg
    14401 gaaagagcgg tggaaattag cgagaagcag ttctccgaag cgattcttga ggctgccaaa
    14461 aaagccgggt ttacgagagg ttaatgtgtt tattgaagca tttgcaaatt tgatgcagaa
    14521 ggcaaagatt ggcacggtag ggacagacat tttctgtcat tacttacctg cctatgtcaa
    14581 atccggtgtc ctgcttatta acccaaatac cggtatcagt atcgatcatg agttacaggg
    14641 cttttatcat gagtcgttca caatcattgt gcgtggctcg tcaattacaa cgactattga
    14701 gaaagcgcgt aagatcattg atatgtttcc cgtagaggaa acggaatctg gagaggtcta
    14761 ttttcgactt gtacggccta tggctatgcc tgtcgtttat ccgaaaaatg atggatcgtt
    14821 aatagaagcc ggtattccag ttgaatttgc tggctattta ttgaattaaa taaataggta
    14881 agtatatact taaatatagc gcaatgaatg cgtgaaatta acggaaaaag gagtttccat
    14941 caatgtctaa tacccatgta aaaaacatca agcttggtgc ctgcaaggta tcatttggtg
    15001 gtgtggatct gggttacacc aaaggtggtg ttcaggttga agtagccact gaaacgctta
    15061 aagttacagt cgatcaacaa ggtcagacag taatctctga attggttcag gggcgtaaca
    15121 ttaccattac cgcacctctg gccgagtctg tgctcaaaaa catggtcgat cttatgcctg
    15181 gttctacttt gagcgaagat gacaactccg tcaccatcac atctgcacag ggcgtaaatc
    15241 tgatcaacgt ggcgaaggaa ctcgtcctga caccgcagga caccaccgac tatgtgttga
    15301 cgctcccgaa agcggcaacc gctggcaact tcactatgtc ctaccagtct gatgatgtac
    15361 gtgtgttctc tgttcagttc aacgcgtacc ctgatgatga gggggttctt ggcaaaatga
    15421 gtggcccaaa accggtgaaa aacgtgacga ttacacctga atctccgagt gtaaaggtcg
    15481 gggcgacagt tcaactgact gctaatttca ccccagatga tgtagttgat aaaacaggcg
    15541 tatggtcatc tgataataaa aaagccactg ttgatcacac tggtcttgtg cgtggtgtgg
    15601 aacaaggttc tgcaaacata tcattcacca gcaatagcgg tagcaagaaa gtaaccaaat
    15661 tagtgacggt taatcaataa tcaagcaact tattaatcta taaattgagg ctcatgatgg
    15721 gcctctcttt taaaaggatt ttaacaaatg acaaaattac tcgatctcga ctccatccta
    15781 cctcctaaaa aaagtgtcaa atttggcggc caggaatatc ccatcgttga aatgacggta
    15841 ggtctgttcg tttccattaa gcagatggaa ggtaaagatc tgaccaatat gtctcctgtt
    15901 gagcaagtga cagcttatgc agatctggtt cgcaaggtta tcccttcagt gcccgacgaa
    15961 gttcttgaaa aactgactgt tccgcaactt cagcagatct tcaccttcgc tatggaagtg
    16021 attgatgaag aaaacgaaaa agcggctggc gaaggggcaa agtaatttcc cgcgatgaat
    16081 ccgggacaag gaccgtctca atagatctcg gattctattt cagtcgtata gttgctcact
    16141 acgccgtgtc gccgttagaa ctactgaaca tccctctcac gatgttctgg atgctcagtc
    16201 gcaacatcga ccgtctacgt gcggaagagg atgtccgcaa cctgcaagtc gctcgcgctg
    16261 ctcaggcaga tggcgagggc gtgaaggcgt tcatggaggg tttgcaactc aggattggaa
    16321 gaccagtcgt aacagataaa gtctacgatc caagccagga tagggcagac cctgacgcca
    16381 aagagcaact gatgcaaata tttggcagag gatgacaagg gaatgtcaca aaacgtagag
    16441 tttatcctgt cgctggaaga caaacagttt acagcgtcaa tcgatcgtgc gggaaaactg
    16501 cttactcgat ttggtgagca ggtaacaaag cctgctcaaa aaatccaaac ctttgaacgc
    16561 tctttgggtt cggtcgcccg tatcattggc gttctggaaa gcaagctcga ttctacggca
    16621 gataaactac aggatgtagc tgtcggtttt gagcttgctt cagatgctac gcgtaaaatg
    16681 cgtggcaaca tcaccagcct caattctggt cttaaagctc tgattgagcg cgtcgatacg
    16741 acaacttctt cagtagataa actcaccgcg tcattacgta aagtgcaatc agagctaaat
    16801 gatttctctg actgggcgac ctatgcaagc aaaagcgcaa gccgttttgg tacggaggtc
    16861 aaagaagcct ctgcgtccgt gagtggcatg aatacgcgcc ttaacaccac gacgaagcga
    16921 ctcagtaatt ggggtgtgac aacaagccag gctgccgagg gactaaaaaa agttcgtgag
    16981 cagatggacg aagttatcgg acgtcagcaa ctcattaaca agcctgtacg cgttcgcaca
    17041 tcaggaagcg gaaatggtgg gggtagcggt ggacgtggtg gttactcttg gcatatcggt
    17101 aaaaataacg aaggcagtat gttctctggc cttcgtggca acattttcct gcttggtgag
    17161 atcggagatg ctgcaagaac cgtcaccagt atcatgtttg gttggcagaa gcctattatt
    17221 gaagctgcgg ccgaaatgga acgtatgcgg gtgatgcttc gagggttgaa taaggagaag
    17281 tccaacccgg gccaggctgc cgctgatgat atgaagtaca tcgtagacat ggctcaaaat
    17341 gcgccgttcg cgatgcaggc gttaacagac tccttcgtga agtttcgttc tgctggctta
    17401 gatccaaccg atggttctct gaaggcgctg gtggactccg ttgctcgttt tggtggtgat
    17461 agtgagctgt tgaaacgtgc ggctgtggcc gttcagcaga tgtccggtaa gggcgttgtg
    17521 tcaatggaag agctacgtca gcaattaggt gaagccgttc ctaacgcgat gcaggcaatg
    17581 gcagacgccg caggcatcac tatgggggaa ctgactaaag ccgttgccag cggtacggtt
    17641 gaggcaaaac aggcgttgtc tctgatgttt gttggtctgc gtgcggagaa tgagaacgca
    17701 gcgaaagaca tgatgcaaac ctacacaggt gcgctggcgc aacttcagac gtcattcacg
    17761 ttatttgctg atcgggttgg tcaggccgga tatctggatt ctctatcgaa ggggatgaaa
    17821 gaactggctt caatcatgaa tagcgccgaa gggatttcgt ttgccaattc tttaggttcg
    17881 gggttaacga cggcaatcga tgggttgcgt cagcttgctc aatggttagc aaagaaccaa
    17941 gagctggtaa ttaatctcgg taaggtcgtg gccgcgatgg ttgcgttcaa actgatgcga
    18001 gcagggatca tgggagtagt tggcgcaggc agcaagatgg ttagcacctt tgccacgatg
    18061 gcgaccgcca tacagactcc atttaacctc ggcgctacag cagtaactcg attcaatcgt
    18121 gcggcacgta tggggctggc tccgatcccc tctcttattt tcgccatccg tggggcgatt
    18181 acggggctta aaggcgcttt tgctggatta acggcgttca ttgcagcaaa tcctataggg
    18241 gcagcattta cagttgccac tgtagctgtt gctggcctaa tcacgtacat gaccatgctc
    18301 cgcagcgaaa cttcaaaggt cgttgacgag attaggaaaa taccagaggc gatgacggcg
    18361 gccaaacgtg cacaaatggc cgaccgcgca gaaaagctcg aaaagcagat tcttgctgat
    18421 caacgagcat taaaaactgg tgagggtgtt aattacgtat ctaactctgc cggtgttatt
    18481 actcacaaag agtcgaaggc tgacatcgaa gcacgcctga aaaaaaatca ggaagagtat
    18541 caaaagataa ccggcacgat agctcttggt gacggcgctg tagcaaagcg tttggctaaa
    18601 gaggctgccg aatctcagat tgagaaaatt cgagcggaaa accagatttt cgcggcaaca
    18661 ttcgtgaaag cccggcagga ggctctggat aagatccaga aaatcaatga tgacggttca
    18721 ctttcagatg acgaaaagaa caagctattg gcaccgttac gtgaaacggt aaacaaaagc
    18781 tatctggagc ctgcgcaaaa actggttgat tcattttctt ctcgtaagaa tgctaccgag
    18841 aagcaaatag cgactcttaa tgatcagctt gaaaaagcca aaaaggatgg aaataccgag
    18901 caaatccaga aactgcaagg cagtatccgt ggttatcagg agcatttgga agccgttgct
    18961 caggaactga cacaggcaga gttcgagaga gataacgcgg ccaaaactgg taagggcgta
    19021 atgtcaaacc aggggactgt tcttgggtta ggtacaactg ataaagctgc tcagaaggcg
    19081 ctggcgcaat atatgcgaaa ccagatggat tctgcgactt atcaacgtac tttgcctgac
    19141 ggcactccga tgatggactt cgaaggtaag ccgattattg ggcctaaaca actcaagacg
    19201 cagcttaatt tgcagaaagc atccagtgcc agctctctgg agaaaatgag cgatgaagag
    19261 cgtgccgcag ccattgccgc actgactaaa gctcgtgaac aggatgccgc agccgccgag
    19321 aaagcagccc aacgatcagc taatgcctcg cagcgtgcgg ccaagaaaga acagacagcg
    19381 caacagaaac tggcagccgg ataccagaag gctctggata aagccgatca gcttatgggg
    19441 caaatgggtg aaagctctaa ggctacggta tcgtttgatc agtctcttcg cgatacaacg
    19501 aaatcgctga cagatttggc taatgcggtt cctaacgagt tcatcactca agagatgatc
    19561 gacaaggcga aaaaacgtct tgaagacctc aaaaatgcga cacctgaata tcgcgagatg
    19621 tttaatcgcc gcaatgttga gcagatgatc tccacctggg caccggagtc ggattccatt
    19681 attagtgctg gctatacgcc gtctcgtgaa gagaaagttg ctgatttcga agacacctac
    19741 aaccgcaatc tcaaagcgtt gatagaactt cgtgataagg cgtctgatcc taaagttgtg
    19801 gcgctttata caaagaaaat caatcaactg attgctgctg gcaataccgc gcttattaaa
    19861 gagacgggga ctgcgacgca gaagttggca ctggaatacg aaaacctggc agagcagatc
    19921 gaaagcacct ggactgattt gtttagtggc ttaactgatg tcctgactga tttcgttatt
    19981 aacgggaaga tgagtttctc cagcctatct cagtccattt tgaaagatat caccaatatg
    20041 gtcgtgaagt cgcaaatcac gctgcctcta atgaacatgt tggggatggg aaccaccgca
    20101 gctggtagtt cacagagtgg taatttgctg accggggttg cttccgccgt tgccaatcaa
    20161 ggggtacgaa tgggcaacac tgttaacggc gacaagtcgg taggagaagc cacgaaggag
    20221 acgtccagtt cggtaactgg attagggcaa acaacacagc agaccaccag cgcaattggc
    20281 actgcaacaa atgcgattgg tagctgggta tcagggctat ttgatagcac tgaagccaaa
    20341 gatgccgaga caaaagccgt gaaggactcc atcttctcga tgcagaacct cagctctgtt
    20401 accggcgcgc tgtctgccgc gtttgcaatg cttggagcta atgcttccgg ctctggtaat
    20461 aagtggttga gttttggagc aacggttgca tctggtcttg tttcagcttg ggctggcggt
    20521 ggtttcgaca gcttgacatc aagctctgct aaaaccgcaa ccagcagtgt ggctgacgga
    20581 actaaaggca ttcctgcaat cccgaagttt gcaaatggag gaatattcgg aaaagacggc
    20641 gtgatcccgc tccgggcata ccagaaaggc ggtattgcta actcgcctca attagcgttg
    20701 tttggggaag gttctatgaa tgaggcgtat gttccattgc ctgatggccg aacaatccct
    20761 gtaacgctca gtaccgatgg tatgagtgga ggcggaaatg ttctttctcc tgtatcaatt
    20821 gagatcaacg tccatagtga cggtagcaca actgaatccg gcgatacaga aagcatatgg
    20881 aacaatgccg ctcaacggat gaaagcaatc gcgcttgaga ctatcgctca agagaaacgc
    20941 cctggcggat cactcaaccc aaacactcaa cgtaactaac tatcgactgc cccggtcggg
    21001 gcagtctcac aaggatgtga gatggaaaga caaacgttta attggtatcc agattacgaa
    21061 tctgaaaaga gcgtaaaacc gaatgtaacg gtacttaatt ttggtgatga ctacgagcag
    21121 cgacaggctc aaggtcttaa tcgtattaaa gaagaatggt cgttaacctt taccagatca
    21181 tacaacgaaa ttaatgcaat cgatgacttc cttactgagc gatcaggtgt tgaatcgttc
    21241 tattgggtta atccaagagg caagcagatt gtagttgtat gtgacagtca tacggtcaag
    21301 agatatcagg ggtactgtgt cttaactgct acattcagac aagtatttga ggcttaagta
    21361 tctggataag taagtactaa tttactatca ttgtggcgct gacaggatgt cagcgcctac
    21421 ttatttcaag gatgaaacaa tgggaattaa agctgatatt cagagcttat ctccctctgc
    21481 actcattgag ttgttcgtac tggatatgtc gaacacaact tcagggggga agctattctt
    21541 ccacgccgga acaaacgaac tgatgcaacc ggtcgtctgg caaggagtga catacgagcc
    21601 gtggccaatc aaagcatcag gctttgacaa aactggccag ggaacgttgc cacgtccaaa
    21661 aattcaggta tcgaactttg ccggaaccgt ctctgcggaa gttcaggcga acgacgatct
    21721 tgttggctgc cgcattattc gcaagatgac gctggctcgc ttcctcgatg ccgttaattt
    21781 taaagacggc aacccaacag cagatccaaa ccaacatttc ccggatgaaa tgtggtttat
    21841 cgaacagaaa actctcgaaa ctcatcaggt tgtcgagttt gaattgtcca gtgtgttcga
    21901 tttgatgggg gtacaactgc cgtatcgtca gatcattaaa aacacctgcc cgtggaaata
    21961 ccgagggcca gaatgcggct ataccggtcc atatttcgac aaaaataacc agcagacgtc
    22021 tatgtctggt gcggattact gcacaaaacg ttatgacgcc tgtaatgcgc gtcggaatta
    22081 ttttgccgac ggtgtgatcc attttggtgg atttattgga gctacgcggt atgggtaata
    22141 aagcaatccc tgagcttggc tctgacgtta tgcagcaaat ctatctctgc gccataaatc
    22201 gctaccctaa tgaagcgtgt ggctttctgg ttagaactaa tggcgacaaa tatcgcttta
    22261 tggaagcgcg gaatgtttcg gagaacccgc agaacacttt tgtaatgcac gttgacgaca
    22321 ttatggcggc tgaggatgcg ggtgatgtta tcgcaatctg gcattcacat actgatgaat
    22381 cagcagaagc atctgatgcc gatcgtgcag gctgcgaagc gacggaagtt ccgtggatga
    22441 ttctggctat tcgcaagaat gttgagggag atgccccttt ccattttagc gagatgaatg
    22501 tgatcacacc tgatggtttc gaaatgccat acctgtgcag accgtatgta tttggcgtat
    22561 tcgattgctg gatgttgtgt cgggactatt tgaagcgtga gtttaacgtc gaactaaacc
    22621 cgaacgcaca cctgcatatt ccatcgtggt acaccggcga taacgacatt ctcgaccaga
    22681 actaccgaaa cgaaggattg gtacggcttg cgcccggaac agaacctcaa cgtggggacg
    22741 tcttctttat ccaatacgga aaaatgcctg accactgtgc ggtttatatc ggcgatggaa
    22801 tgattcttca tcaccagatc gaccgcctta gttgtcgtgc ttattacggc gggatgtatc
    22861 agaaacatac gacgcatcac ttgcgtcaca gagacttgct caagggagat gagacgtgtc
    22921 tgagttagtt catgtgcagc ttggcggccc tatggccaaa cattttggcc gccactggca
    22981 tctaaaggtg cgcaatacaa aacaggctct ggatttaatt gaggccaata agcctggttt
    23041 taaagcatgg atgaagcgca atatcaaaac ctatgacaga taccacatcc agatcaccaa
    23101 taaacagggc cacaagtggt ctgtggacga gagtgaatat cagatgatgg ggcagtctga
    23161 caacattgcc aaaatccgca ttacccctgt tccgcgagga agcggcggat ctgcttttgg
    23221 gtggtttcag acggtagtag gggccgcttt gttggttgta tcggcggtaa tgatgcccgc
    23281 tcttgcccct ctcggtttgt cactgatgat gggcggtata gcgcaaatca tatctccgca
    23341 agccactaac gaaagtgtga ggcaggcgga taactcgaac tcttattact ttgatggacc
    23401 tcaaaacaca acaaaccagg ggaaccctgt acagcttatc tatggcgagg aaattttagt
    23461 tggctcacag gtcgtgagtt cttctatcac catcgaccag cttatgtaat caaggatttt
    23521 tggacatgga acagttcaag aagaaaaagt tacctctatt aattgcaggt tctggtggca
    23581 aaaaaagtag caaaagctct agtcgcacac cagttgaagc agacgatacc gtaaattctc
    23641 gtgctatggc ctctattctc gatcttcttg gagaaggcgt aattggcggc ttggtaaatg
    23701 gcgcaagatc tatttttgtc gacgatttgc ccatagtaaa tgaagacggc tcttcaaact
    23761 ttagcggaat ttcatgggat tttcgagacg gctcgcaaga ccagacacca atgtctggct
    23821 ttgatttcgt cgaaacacct aaatcgatca atatccaact aaagaaatcg cattatgtga
    23881 cggtttcaat agataacgat gaggccgatc gtgttcgagt catcatgaag ttcccttctc
    23941 tgcgaagaat agaccaaaaa acgggtgata caaacggcac taccgttgag tataagttcc
    24001 agatctcaaa tggagattca acatttgtag atgtggtcgc agaaggtgaa aaaaacgttg
    24061 gtattaagtt aacagcgaag aaaaccggtg cttattatcg tagttatgag ctgaagttac
    24121 ccaagccagg tcgggcatac agtgttcgag tagttcgtat tactgatgat aacagcggtc
    24181 agtatcttta taacgataca tgggtggact caatcggtga gatcgttgat acaccgatga
    24241 actacccaaa ctctgcgttg gtgggactga aggttaattc tgagcagttt ggcggctcta
    24301 tgccttctcg ttcctatctg gtgcggggat taaaaattcg cgtaccatca aactacaacg
    24361 aggctagcaa cacttatgat ggcgtatggg atggctcttt taagctgttg tcatcttcaa
    24421 accctgcgtg gattctcttc gacttgctta ccaattctcg atatgggctt ggtcagtatg
    24481 tgtcggaatc tatgattgat ctcggccaac tgtaccagat cggtcgatat tgtgacgaag
    24541 aggttgatga tggctttggt ggtaaagaga aacgctttgc aatcaatacg cagatcacca
    24601 gtagacagga tgcataccga ctaattcagg atattgccgg tgcattccgc ggcatggtgt
    24661 tttgggctgg tggcatggtt aacatcatgc aggatagccc atcagatcca gtaatgatgt
    24721 ttaccaactc gaacgtcaaa gatggattgt ttacctataa aggttctgcg cgtaaagatc
    24781 gcccatccgt tgcgctcgta acctacaaca acaaggagga cggttataag caaaacattg
    24841 agtacgttga agatcaggac gcaatgcgcc gttatggtga gcgtaaaaca gaagtcgtag
    24901 catttggatg tacaagccga ggccaggctc accgagttgg tttgtggctt ttgtataccg
    24961 ccagaatgga gtcggatgta attacattta ctgccggttt agacgcctca tttctgatgc
    25021 ccggtgaaac cgttctgatt cagaacaaat atcgtgcagg taaacgtaac tccggtcgaa
    25081 ttgtggagtt taccaaaaac agcatcactc tcgatgcgcc tgtgtcgtta gctaaaagcg
    25141 gcagctttat tcggatattg aatcaggaag gcaaaatcgt tgaacgcgat gttcttgaaa
    25201 ctggcgaaaa cataacaaag gttacgtttt caaaagctct gtcgtcagcg gaaacgcctg
    25261 ttttgaacgg tgtatggaca atcacagaac cagatctcga acctatgcgc gttcgcatcg
    25321 ttaacatcgc tcagggggaa acgtcgggta gctttgacat caccgctgtt gagaacaatc
    25381 cgtctaaata tgaggcaatc gacaatggtg caacgcttat cccgcagaat acgacggtat
    25441 tggacccgac ttactccaag ccgtctaatt tgcaaatcac cgaagggact tatctctcaa
    25501 gcccaggcaa cctgtcagta aaactgactg caacatggga agggaaatct ccagagtatt
    25561 ggatcagttg gcgacgttct gatgaaaaca atgtatcgaa ctggcaatcg gcacgtgtaa
    25621 ccgaagagca atacgaaatc gttaatgtcg cggagaatgg acgctacgac ttccaactgt
    25681 atgcggtttc attcaacggt aaaaaaacag agattatcag taccgtttat caggtgctgg
    25741 gcacaatgac accgccggat gcaccaacgt cattaacggc cgttggagac tatcgtaatg
    25801 tgatactgaa ttgggttaat ccagattcgg tagaccttga tcacattaat gtttacgcat
    25861 cccagaccaa caatctggat acggcgaaac tgatcgcaga gtctgcaagc accaccttta
    25921 cacattctgg tcttggggat agtgaaactt ggtattactg ggttagatca gcaaataagc
    25981 gtggcatgtt gagtcgccca aactcaaatc ttggtacaga ggccacaacg cgagatgttc
    26041 tttcattttt agccaataaa attacctcgt cagaacttgg ccaggcttta aatgaggaaa
    26101 ttgacagtaa ggcttctcaa caagctgttg atgaaatcaa taatcatatc gatcagagcg
    26161 tcgaatctct ggaaggtgaa atttccgcat taaataagga gctaacgagt agtatcgatg
    26221 agttcaaaac atcgtttact gaacgcagcg agacactgga gaatgcgcaa agcgagctta
    26281 gaacggaagt ttctagcact cgggaaaaag tagatgatgc attacgacaa gttgaaaatt
    26341 caaatgcagc acttattgaa ctacgtaata cagtatcaaa acaaggtgat tctgttgctg
    26401 gtgtcgtaga agcagcaaat gcggcacttg aaaatgcatc atctttaatt gccgaagagc
    26461 gtaaagttcg tgcacaagca gatctagcga atgctaaaaa gatagaaacg atgagatcat
    26521 cggttgatga aagtgttgcg gctgtagaag aaatgcggag aacggttgct gaggtaggaa
    26581 gagtaagttc agaaatcaca actaatcttg aagccatttc taagacaaat atcgatcttg
    26641 cgttgcggca ggacgaagat cagcacagac agctggtaaa taacgcgaaa attgccacga
    26701 ctcaaaagat tcttgtcgat gatgtgtcag caatggcgtc taaggttgaa gagatacgag
    26761 ccgagattgg tgacaacatt cgagcatcta ttctcgaaga agcgacagcc agaactgatg
    26821 gtgataatgc gttagccaat cgtgtgacac aattacagag taaatttgaa ggtgatatca
    26881 gtgccgcaat aaggaccgaa caatctgcaa gagcatcggc caatgagact ctttcaaagc
    26941 agatcacaca actggagtct aaagtaaata ctgatatttc aaccgctatt agagaagagc
    27001 aacaaacaag agcaacgcaa gatgaagcat tatcaaggca aataactcaa cttcaaacaa
    27061 aagttaataa cgatatatct gctgctatta aaagtgagca acaagcgcga gttagcggcg
    27121 attcagcgtt atccacacaa attaatcaat tgcaggcaaa agtagatgat gacatatcag
    27181 ctgctattaa tgaggaaaga gaagccagag catcaggcga tagtgcacta tcaagagaag
    27241 ttagtagttt aaaaacaaaa acaggtaaag acattgcagc ggcggtagca gttgaaacca
    27301 aagcaagaac tgacgctaac gatgcattat ccagacaaat ttcttctctt actacgaggg
    27361 ctaataatct tcagtcctcg atagagcgag aatcgacatc tcgtacaagt caggattctg
    27421 ccattgcaaa agacttggca agttttaaaa caaaaactgc taaggatata agtgcagctg
    27481 ttgcggttga aactcaagca agagctgccg ctgataccgc attgacgtcg cagattaact
    27541 cactaaagac acaaacaggt aaagatataa aggctgctgt agcagaagag acgaaagcca
    27601 gaactgatgc tgatgcagcg ctttcgacaa gggtaactaa tctccagtcg caaacaacaa
    27661 attatatcaa tgcagcgatt acgtcagagt caaaggccag aacaagtgca gacaacgctc
    27721 taggaaaacg aattgataca ctgaaagcgt ctgtagatgg aaatgttgcc gttattcagc
    27781 agcaggcttc tgctatagct gacgctaata gaaaagtatc aacctcgtac acattgaaga
    27841 tggaaacttc aacgagtggt gggcagaagt atgtagcggg cattgcgctg ggtattgata
    27901 caacggggct ttctcagttt ttggtgcggg cagatcgatt tggtttggtc aacaatgtta
    27961 atgggaagat cacaacgcca tttgttatcg aaaacagcgt tgcgtatatg aacggtgctt
    28021 atatcaaaga tggcactatc acaaacgcaa aggtaggtga cttacaatca acaaacttcg
    28081 taagaggacg agccggatgg agatttggca aaaacggaac gcttgagatt aacggtaaca
    28141 gtggaggaaa tgggagatta gttatcaatg ggcagagaat cgacgtttac gacgacaata
    28201 atgtactcag ggtaagaatt ggtcgacttt gattatggaa aaaaatattt ttatcgataa
    28261 taataggtag ttacatactt aatggtgggc aaggatagcc cacctgatta aaggagcaga
    28321 ttcaacatgt ggtacaggga aggtactatc acatttacac aaggcagcaa tactctggtt
    28381 ggtgctggga cagcatggaa cgttacggcc aatggtgtgt tgcctggcat gattgtcgtc
    28441 gcacctgata ataaattgta tgaaattaaa agcgtaacaa atgacacaac cttaacgttg
    28501 gttgaaaact atactggtga aacgcaaagt gatataccat gtaggatcat cactacctac
    28561 gaaggagatt tgacacagtt tagtgcgaga tttacggctc ttttatcacg aatgtctggt
    28621 gatgcaaaaa cagttcgaaa ttggttaact gccgtagatg aagttactct tgagcgagaa
    28681 gatggcacta atgttaatgt aaaacctctt tctaagatta tcgcggagca taataagcac
    28741 gtagaatggt atgaaaataa tactaacgca attaattccg caggagataa agcaaaacag
    28801 gctgcggcca gtgcgtcggc tgcggcgaag agtgcgaatc tagcaagcag taaggctact
    28861 caggcatcta atagtgcgtt ggatgctaca gcgtctaaaa atgcagcaaa taaaagtgca
    28921 tcaagtgcta aagcatcaga aacaagtgct ttagaggcga agaacgcagc tgcaatttct
    28981 gcatcaacag cttccactaa ggcaacagaa gccacaagtt cggctaatgc tgcgttggtc
    29041 tctaaagagg ctgcaaaatt atctgaaatg aatgctgctt ctggtgctgg cagtgcatca
    29101 acttctgcaa atcaggctag agagtcggca aggtctgcga agacatccgc taatcaggct
    29161 tcaacttatg cgtctgctgc tagagcttcg aaggatgaag ctagccgaaa gtctgctgaa
    29221 gcctcaacta gtgctagtaa cgcatcatcg aaatcagctg aatctgcggc taaagcagaa
    29281 gaagcaaagc aaagccaaac atcagcactt gcatcggcag agcgtgcaga gatagctgct
    29341 actagagcag aaagagcttt gtcagcgata tcattacatg atgccagttt gactatgaag
    29401 ggtattgtca aattaagcag tgagacgagc agttcgtcag aagaatttgc agcaacacca
    29461 aaagcagtaa aggctgcgtt tgatcttgct aaatcaaaat atactgcaaa tgacgcgaca
    29521 acttcacaaa aagggctggt tcaattaagt agcgatacaa ataacgcctc tgaatcattg
    29581 gccgcaacaa caaaagcggt taaaagtgta aaggatcttg ctgatagaaa agcagcaatc
    29641 aacagcccag catttactgg cacaccaacg gcaccaacgg cgactcaagg cactaataat
    29701 acgcaaattg ccacaactgc ttatgtaaga gaagcgatct caggtcttgt aggatcatca
    29761 cctgaagcgc ttgatactct taatgagttg gcaagggctt tgggtgatga tcccgatttt
    29821 gccacaacaa tgacgaacaa gttggccaca aaacaacctc ttgatgctac gctgactgca
    29881 ctggctggac taacaacagc cgctaataaa cttccttatt ttacagggaa tgatacagct
    29941 ggattagcaa caataacttc tgttggtcga gatattcttt ctaaagcaag tgtacaggcg
    30001 attattcagt atcttggatt gaaggaaaat ggaacaagtg gcgataaaat acctttattg
    30061 agtacagcca atacatggag tgcaaagcaa acatttagta atggtcttgc aggctcactt
    30121 aacggaaatt ccgataccgc aacaaaatta aaagcagcac gaaaaattgg cggagtaccg
    30181 tttgatggtt catcagatat aaatctacca ggtgtaaact caacaggaac tcagagtact
    30241 agtggaaacg ctgcctctgc cactaagtta gcaacacctc gttcaattgg tggaatgtta
    30301 tttgacggca ctgcaaatat tgacttacca ggcgttaaca aggttggaaa tcagagtaca
    30361 acaggtaatg cagggagcgc aacgagactg caaacggcaa gaacaatagg tggggtatcg
    30421 tttaatggca ctgcaaatat tgacttacct ggtgtcaata aagctggaaa tcagaacacg
    30481 tcaggtaatg ctgcaacggc gacaaaactt gcaacggcac gaaaaataaa tggggtcagt
    30541 tttgatggct caaagaatat aagcatcacg ccaaaacaac taggtgcagc aaaatatgtt
    30601 gctaatcttg ctacggggaa tactcaagag tggacaactg cacaatttgt cgcatggtta
    30661 aattcacaag gggcatttga tgcaactcag tggagcacac gttgtagctg gtcttatggt
    30721 agtaactgtt atataggaaa tgcatcaacg ggatgtggag ttatacctct tgctggttct
    30781 gttattgagg tgttttcaac cggtacaagt aattacacta tacgcattac aacaccttca
    30841 acatctggag gtgcatctgg tgctgttctt aatagtgagt ttatttatac atacaacggc
    30901 gataactata gcccaacatg gcgtcgtaat tacagttctc aaaataaacc aacggcttca
    30961 gagataggcg cccttgcctc aaatggaaat gcagtttcgg ctacgaaact gaaaacagcg
    31021 agaaatatag ggggggtatc gtttgatggt acagcgaata tagatttacc aggcgttaac
    31081 aaagctggaa atcagaacac atcaggtaat gctgcaacag ctactaaatt aaaaacatca
    31141 agaacaatag gtggagtatc atttgatggt agcgcaaaca ttaatcttcc aggtgtgaat
    31201 acgtcaggaa atcagaatac atctggtaac gcagcatcag caactaagct acaaacagca
    31261 cgtactataa atggagtaag tttcgacgga acaaaaaata ttagtataag tgccgctcaa
    31321 attggtgctt tgcctatcgg tgggggtacg ataacaggta atttgactgt aaatggaaat
    31381 ggctcattta atgatgttca gatccgctca gataagcgta acaagcgtaa tctggtaaag
    31441 ctagataatg cgttagatcg tttggaggca cttactggtt atctttatga aatccaactc
    31501 tctgatgata gttggaaaac gtcagttggt ttaattgctc aggatgcaca aaaagcattg
    31561 tctgaactgg taactgaaga cacagacgtt atatctggtg aaaaacgtct gtgtcttaac
    31621 tacaacggca taattgcatt gttagtcgag ggctttaaaa cacttcgtca tgagattaaa
    31681 gaacttcggg agaagtaaac gacagctgtt ggagtttctg gtttctactg aatttaaatt
    31741 gtgggggtga tactcacccc acgcattcaa aaggaggggt aaaatggggg tagcgtcggg
    31801 atgggtcggt tcttcggcta agagcgaaac gggcgaacaa tggatgagcg ctgcgggtac
    31861 taagttagga ttgggtaaac cttttatgat gagtcaaatg gtgggacgat ctatgggctg
    31921 taaaattgca accgcttact ataaagggaa tcctcctgat aaagtcgaaa actggggggc
    31981 tgtaggttca gattggccat tggctgaaaa gaaccaaggt acaattgctg gtgcttcgag
    32041 ctgtggttta ggtcgtttag tggggatcgt gcttacgcaa gctcactacg ttccaggttt
    32101 accgcccact gggggtttat acatcgcagg gggaagggca agcaatatta cagtaacagt
    32161 tggtggagtc tcacaagttt ttacttacaa tagtgttatt aacggttttc actattactg
    32221 gcaaggaaca cctagttcgg cctttataac ggcaatgaaa aggacggggg ttacacaaga
    32281 tttgaaaatt agttaaaata taaaaggtta tatattattt aaatttcagt tgatatttaa
    32341 attacataaa attatgtaag gggatattaa aatagattgc tcatggagga agcatggcta
    32401 atgacgtagg aggcgtcact catgatcagg ttgagagaat agctaaaatt gtcgccagag
    32461 aagttgttgg taaattagga aaagaaatca gagaggagat agttcatgag gttcatgagc
    32521 agttgaaaaa ctattttggt gacatgactc cggctcaaca cagtattcaa cattccaatc
    32581 tggataaact acttaatagg ttagattcga tctcaagcgg cttctttgga ggcattattt
    32641 ctaaagtgac gtcgtttatt ataaccgcac taattttagg attagccgca tatggcgtaa
    32701 aaaatggact gcaataacag gagaacaagg atgaagactc cgagaggcat tcgtaataat
    32761 aaccccggta atcttgataa aggatcaccg tggcaaggtc tggttgcgaa tccagacgaa
    32821 cctcgctttt gcacgtttaa agaccctgtt tggggtattc gtgctctggc agtgactcta
    32881 attacctacc acgataaacg tcgcgccaaa gacggctcaa gtattgatac cattcgcgaa
    32941 gttattgaac gttgggcacc gccgaatgaa aacaacactg acacctacat taatgaggtg
    33001 tctaaagctg ttggtgtaac cgcagacatg atcatcgatc tgcatgatta cgacatcctt
    33061 cgacctttgg ttgaggcaat cattcgccat gagaatgggc gaggtccgct aaaaacgctg
    33121 aacacctggt atgcggcaga agttattgag gaaggtctgc gtcgagctgg agtcgttaag
    33181 ccggtgaaaa ccgtgaaggc tgttcctgta actaaagaaa cagcaggcgc aactgttaca
    33241 gcaggtattg gtctggcgca gctggccgat gttatgccgc aggtttccgc tgctatggat
    33301 aaagcacaag gtcatatctc tagtggggat acagtacgca tcatcttcgg tattgccact
    33361 attgttgtgg caggattcat tgcctggtcg caggtaagaa aacaccagaa agggatggcc
    33421 taatatgcta ggtagcctga tgataaagct aaaagttgct ttgattactc tggctgccgt
    33481 tcttttcgtt ctggtcggcg cttacacgat gggcggaaat gcggcgcgac gagcaatgga
    33541 agagaaggca aaacaggaag acaggaaacg acttcaaagc acagtggatg tcaaaaatga
    33601 aacacttgat gagttacggc gcaaagatgc ttctactgtt catcatgagt tgcacaataa
    33661 gtggttgcgt gattaagcct caatcctctg gtgtgctttt ctgcgatgcg gctacaccgc
    33721 tatacatcag ccgtgatgat ctcatgaccg aagagactga aagggaagta ctttttcaca
    33781 atatgatagg ggagcgattg tgtggatggg gcagaaaagt gccataataa aagcggaatt
    33841 ttctaacata aaatttgcat attattagag cacaaggtag atttgaagca tggatgtcaa
    33901 gtaaatcata ccttgtttta ataaatcgac gcatactcgt agcctctaaa ccgacaaggt
    33961 cgccgctatg acataaagtt attaccggct aaggtggaaa catgcatact gcgcttaacg
    34021 catctcgttc tattcagacg ataagagact gtaccaagat gatcgcatcg gttccgttgc
    34081 ggcattggaa tgactcgctc caaattcgtg aaatgaacaa accgggtatg cttaaggatc
    34141 agcgtttcat cgttaacctt aaaagtgacg ttgaagaagg caagtttacc atcaaattgt
    34201 ttgcagaaat gatggtgctc aacaatcggt tacgttttct tgttaagcca gaaaaacagt
    34261 ttcccgatgt tgtgcgttgt gaacaggatg ctttctatga gattgaaagc aaaatagcta
    34321 aatttatcaa agataaatac cgcgtagatg ttaaaatagc gaaactggat tcatctacgg
    34381 atatgcggct tgctaactaa atgtcaccat cattcatagc agagtattca aaagtactgt
    34441 ctgatttccc tgaatgtgaa gcaagagaga aaatcagagt tggctacatg gttaactctg
    34501 atttttctgt tttacggctc acagagtggt ccttgatcgg gtctaattat gattactgtt
    34561 tgacagtgtc atttgtaggc gaccctgatg cagattggct tcaagaacat gcaatggatc
    34621 ttgacgagca ggggcattca gaagaagaga tagttcatag tgttcgtgaa ttagttggtg
    34681 atgagcaaga agatgatgac ggcattcaga tcgccaggtt cgtctacaaa cgattccagt
    34741 ttcgctcaca aaaaggctct caccttgggg tccagattaa aggggcattt gtaacccctt
    34801 caaaggaaca aaagggactt gctagaagag tttacaattt tcttctcaat tggcatgacc
    34861 atgtagtatg tgatgaccat cagacggttt acggtgccag aatttgggct gtaggtatgc
    34921 ttttggtcgg tcgtgtccaa atctatgaca atttaaataa agaatttata gatgtccttg
    34981 ttgaaggtgg tattgggcag aatggcgtca aaccgtggga tgcgctgcta ttgaacagcg
    35041 aggctcagtt acgtcactgg aaccctatgg ctgtcagcat agacccatct agtcagattc
    35101 tcaccatcat ctccaaagaa gaccgccaca ttgaagtggg cgttacaaca tttgatgcca
    35161 ggtctaccaa ccactaattc ctaaacccgc tacggcgggt tttttgttat taagtcctta
    35221 ccaaatctat gtacctatca ttaaaccttt acacagcagc cgtaggcatt taggctatat
    35281 cacatataag aaaacaagtt gtttcagaca ataataatag acgcaaagga aactctccaa
    35341 tgaccaagat ctttgtggtt ggcggcacaa agggcgggcc tggcaaatcc accgttgccc
    35401 agcaaattgc cgtttgcctg aaagtcaaaa agaagaagaa ggtttatatt accgatatag
    35461 atattcagcg cacgacaacg agctggtgtg aagaccgtcg acagaacgaa gaccttgagc
    35521 tgattccttt tgcatacgtt caggatgaca tcattaagca cctaaaatcg cttcagggta
    35581 gagctgagtt tgtagtggta gatgctggtg gcttcgactc cgaaattcag cgacaagcga
    35641 tgctgatggc tgacgttatc attatcccgc tgcgtcctaa gcgtcgtgat ttgaaatctt
    35701 tacgtgacat cgatcctatt atcgacaatg ttcgcaatgt aaacgataaa gtgaaggtcc
    35761 gcgcggtcat gaaccagtgc ccggctttgc catcacaagt gtctcgcatt ctggcggcta
    35821 aagagattgt tgagacgttt ggcatcgagt ctgcgccagt caatctatat aaccgcaacg
    35881 tctatgatga tgcggaagag tctggtcgtt ctatctttga aatgaccggt agcgagcgcg
    35941 acaaaaaggc ggaagccgag tttgaagaat ttgtagatta tctgttgagt ctggaggaag
    36001 aagaataatg tccatgaaaa tgggtgacct agcaaagcgc aaagagtctg atgcaccggc
    36061 taagaacaca actcctttgc gccaaccagt cagaccacag ggacgcccga ctcgtggcaa
    36121 agagaaaatt aaaagccgca caatgtcact ggaggacgaa tacttcgaac tgctggagat
    36181 gatgaagttc atccctcgct tcgagaaatt cacccgctct gatgtgattc gtgctgctat
    36241 tttccatctg gcagagaagt caccgcagga aatcgaggac atcgtgaagc ttaacgaggc
    36301 gatcactgct gccgatgtca cgatgcgtac cgatgaaatt aaacgcgagt taatgaagaa
    36361 aggttgatat aaatctctaa ggctggtctg cattggacag ccttagaaat atttcgttct
    36421 atgaaagcta ttcgtagtta agctcaccga acagactgaa catcaagtca ccatccaaaa
    36481 tttcaaaggg caactcaaca tactcatcct tatgtctcgg aattgcattg ccgtcgttag
    36541 gggcattctt ggtataacct tttacatgtt taccgttttc ttttgtatac ggcagcacga
    36601 caattcgacc gtgatttttt gaaatgatag ttccttcttt ccagagcgta ttgcctcttg
    36661 aggtggtttc gctttcgcta gcaactttaa cactccaacc tacctgattt ttgtcctcaa
    36721 accagaaaac aaagttgcca acaaccagta ccttttgccc attcctgaga gcttcatcca
    36781 gcattctttt tacgctggcg agttgcagta accggttagc acgcggcaga agcattgagc
    36841 gaatagtcgc ttttgttttt ccccaatgtg cagcacctga taaaccaaaa cctctggcta
    36901 tgcgttcttg gtagcagatc ttcgctgtct tagctttgtt gttatatgaa cattcccaag
    36961 tgttaggagc aacgagttga agtgaaactt ggaatttggg aagaaaaaca aaatgtaagt
    37021 aaggcatctc gcttagcacg tcaaaaaggg gttgacgcaa agtttcgtaa tctgggttaa
    37081 ttattaactc atttaatgtc actcgcggat cgttggcgtg tgccttaatt gcttcgttga
    37141 gcattatata ctcttcatcc atgagacgtg atttggcttt gagtgctttg tcaactttca
    37201 aacgcaatga cttcttttga aacatagata gaggaagttt ttcaatgtca aagctcacgt
    37261 ttttattcag ctctaatgcc gcttttcttg cttcaggata gttatcgtag tcgtcatcaa
    37321 cagcgtggtt tggcatacca cgatcaacat atcgccgata ccaagcaagg gttccatcat
    37381 tacggagttt cagacagaac atcgaccatt tattccgttc atatttaggc tgaggccaaa
    37441 aagccagttc gggaaggcca tatcctggaa gtctattcat gcgcatggtg acaatatcca
    37501 cgaggctaaa acactaattc taacatgatc tcccttgata aatctgtcaa tgacatagca
    37561 tctggagttg gagtcaaatt atgttgttct tggtatccat taggagcctg tttctgtata
    37621 taaataataa gtaacttatt aaatatatac ggaagcaggt cttttaaaag acaccaccag
    37681 aacaactccc ttccgttttc acttccaaaa actgacacca gtcgctatca tccgtccatt
    37741 gtgataagta agtaactacc taccaggtga gccacatgag ccaaatcttt ttcgatacca
    37801 tcgacaacga ccagtacgac ttcatgacag agtggaatac cgctgttatg gacaagtggg
    37861 tcgctgaaaa cattggtttg tcgcgctgta aagacgaggc tgaactcttc gagacgaagt
    37921 ggtttgatta ccgagacatg catcctctta tggccacctg tctgtttacg gaggcataca
    37981 aacgtcagta ctcaaatatc atgctgacgc acggtcgcga acactttgaa acagctccgt
    38041 tcaccaccgg gttaaaacgc ctgccttatc aggagttgtc gactgccaat aaaacgtctc
    38101 tatggaaagc acgccagttt gctgaccgct attgctgctc atacgactac tttatctcca
    38161 ccgttctttc cgcagctgca cgacggctgt gggacaagct gccgcgccca cagcatttgt
    38221 ggcagccaga gctggttgag atattcgaag agaaattagc cagacgcgca acaacccgtc
    38281 tggatgactc tcttgttagc tttaagcata tgggagacat gcagttcaac ccgattcagg
    38341 aaagctattt tgagtggatt ctggagtgtt tgcgcaccat ccctcgcagc aagcgcatac
    38401 gcgcaatttt ctccgctatc tggctaatgg aaatcgttcc agagcgcctt atttccgccc
    38461 actttccaga agaactggaa gaagcacggc ggtttattga tcccctatct aattaactaa
    38521 tactagaaaa caatttgttt aaaaaacaaa ggaaagcaca tgacagaact ttgccataca
    38581 ggacgcgggc tgtccgaaga gtttgatgaa gatttccaga acagactgac ggcatatttt
    38641 tgtcgtgatc acgagtttct tactcgtgcg ggagatctgg ttgtgcctag ccaatttgcc
    38701 aatgcggcca atgccatatt ggttaatatg gtttcgggct attaccgtat gtacaagagc
    38761 gcgccctctt catctgcaat tctggatatg cttaagcgtg cgaaacgcga taagactatc
    38821 cgtgaggaac tattcgccga tgttgttgct gcgtttaagc gcattcttgc agaaaaattg
    38881 tccgatacct cgtacatggt tgaccaggta tcaaccttcg caaaaagtgt agcgtttgat
    38941 gatgctctga ttaaggctgc tgaactgaaa gagaaaggcg actttcaggg agcgatggca
    39001 atcatggcta aggttcagca gattggatcg aacgaagcga ccggaatcta tgactactac
    39061 acctccgcaa gtgagcgatt gaaagcgcgt gaatatgagg cttcagagga gtatgtgcca
    39121 aacagcatta caactggact ccctctgctc gataggttgc tgtaccaaaa aggctgggcg
    39181 aagcgtgaaa tggtgctctt catggggttc gctaaatccg gtaaatcgac cgcaatgggt
    39241 gagttttcca taaacgcaac gcttgctggc tacaatgttc tgtatctctc gctggaggtt
    39301 cacaccacca ttttatccga ccgttttgat gcaagattgt cggagacaga aatgtccaag
    39361 ctggtggaac ggcgcgatga ggttcatcgt aagttggcag agttgggagc cacgaagggg
    39421 attggtagtt tgtgggtggt tgagcgtccg tcaggaagta tgtcaccggc agatctggac
    39481 cgtatgctta acagcatgaa agccaacggc atggtgcctg acatggttgt tgtcgactac
    39541 gcagatttga tgcgtgccag ttatgacctt cgtgatgatc gcgccaacat tcgtagtatc
    39601 tacaccgatt tacgtgctct ttatgacaag cataacgttg ctgggatcac ggcatcgcag
    39661 acaaaccgtg aaggtggcgc gtcagaagtt gccacaatga tgcacgctgc cgacaacatc
    39721 gaaaaagtac gtattgctga cctggtaata acgatcaaca aaaccgaaga agaagaagcg
    39781 aaaggagagg ctcgtctcta ctttgctggt tcacgtaacc agcagggagg gatcagcatt
    39841 cgcgttaaac aaaacctcga acaaatgcgc ttcattgagc gaatcttaga cgttacctaa
    39901 aaaataagcg tggagaacac ctccacgctt gattcattgg tgaaacaact tttcttttgc
    39961 caaaccacaa aagaaaaaca catgagcctt tatgttatat caacatttag attggtcaca
    40021 atattgcctg ttaaaagtgg aattatcgtg agcgagctga aagagctaat tgccgaatta
    40081 gattttgaac aatggttgga tactgaaggt atcgtttatc gacgtggagg cgtgagtact
    40141 cgcggtcgtg aagtgaatat caaggagtgt ccggtatgcg gcagctccaa ctggaaggta
    40201 tatttcaatc tgaccagtgg cgtcggcaaa tgcttcgctg gtgatcatcc cgaagagatt
    40261 cagttcaata agctggtctt cctcaagcac tacagcggta aatcacgacg acagttcgag
    40321 gaatatgtgc agaacgctct tctttcccag gggtgggcac caaagaaaga agagctagtg
    40381 cttgcaagca cagtcgagtt agaggggcca gttgcactcc ctcgtcatta cgagcttcct
    40441 atagatggcc gtcttccaga ttatctggtt gaacgaaaca tatcacctga aatggcaaag
    40501 tattttgacc tacgatactg cgtcgaaggc aagcacgctt atgtagatcc gtatacagat
    40561 caggtaaaag ggcagatatt cgatatgcga atactgatac cggtttacga tctggatggg
    40621 gtaatgaaga catttcaggg acgagacatt accggtacag cagaacgccg ctacctcttt
    40681 cctatgcagc ttccagcttc aggtaaattt ctctacaacg gccataatgc agtcggcaaa
    40741 cagactgtag ttgtctgtga gggtgcgttc gatgttatgg gggtcaaacg agctattttc
    40801 gacgaagaaa cattacgtga ttacgtggag ccaataggaa cgttcgggat gcatctatct
    40861 ggcaacacca ctcaggatgc agaagatcag ttgggcgcgt tcctgacgct caaggcgcgt
    40921 ggattacgta atgtgatcat gatgtgggat agtgaaaagc aagctatacg caacacgatg
    40981 gccgcagcca ggcgactgac cagtatcggt cttaatgtca aagttgcgtg tttgggtgag
    41041 gaaggactcg acccgggcgg tgcaacgcca gaacaaatta tcaaagccta ttatcgggca
    41101 aaaccgtata ccaaacagtt ggagttgcaa agcaaggttt tgggcattaa ggctctatca
    41161 taacaatcac ctttaaaata agtagatgat tacttatctt tctgtgagaa tactttcatc
    41221 tgttagctag gagttggtat gaaagacgaa attcagaaat tagcctgcga catcattgat
    41281 aaaactggtt tagaaatcag cgagagcaat cgactagaca tcattgaaaa agcggtaaaa
    41341 acagcaatgg atcatatcgc cactcgtttg gtcgagatcc cgctaccggg gctaccttat
    41401 ctgaaggtta agttacacgt atggggtgaa ccttcttgtg cacgacgttc tgcattagtt
    41461 gtttttatta gcaaagaaaa cccgctcagt cttaaagtgc aggttggagc atggcttgat
    41521 ggcagagtga tctacacaaa taccgttttt tgtctttcaa acgacgaaac tattgaagcg
    41581 gccattcaag aagcagttct agcaatgcgc agcagcggtt tgatgaagaa taactacgaa
    41641 gagtacttgc gttcgataag tggtgaaaag acattatctc tgaaagcaga tttcgttacc
    41701 ccgaaaaatc tgttggaagt cttgcttaat aaaggggcta atgatgccgt aaatgtaatc
    41761 agagagagtg agtatgcgtc tctttgcgac atgtgcaaaa gccagttgga tctggtgcat
    41821 atcgttattg atgctgggaa ggcatgtgat ggcgtaatgg cggaatttgc ttggaaggtg
    41881 gtcaggattg ctaacgaatt accgatgata gagcaagagg ctaaatcata cgccaccaat
    41941 catgtcacag agcttcttgc cccctatcgc ttagaaagca atcagcgcaa gatgattagc
    42001 tggggaagtt ggtaatctct ccgcgcgtcg ttttttacgc aaataacgat aggtaagtac
    42061 aagattattt atggcggtag ttgtgaaagc tgatttgtca aaaatcccct ctatttcagg
    42121 aaataatggt tattcacttc gttgtgagga agtaaagata aacggtgagt cggcatattg
    42181 cagctactcc gtttgccagc acaccattct tgccttcaaa gaaaaccgtc ttcctcgaac
    42241 ttcattccag tcgtgcgcaa ccgctatcaa agcaggcaaa tgcaaggcgt taaaaatgat
    42301 ggttgaagag attcgtaaag gagaatctct gtatttcgaa gatatgaccg cgctcattaa
    42361 ggaggttgaa gaacggaata aacaagccag aactttaaaa cgaaaacgtg acagtgtaac
    42421 gattaatagc atggttaaga agagcaccac atcacaaaca gcgatcactg acgtgtatgc
    42481 ggcgttgctt gaagaaacaa caaaagaaac acatgagcaa atcgatcaac atatggaggt
    42541 aaaacaacaa tgaaaaagtt gatcgcactt aagcataagc tggacgaaat gaaagctatg
    42601 ggaaccaatg caaaaaaaga ggcattggcc aacatggatg acttcgagca aagcatggtt
    42661 tcattgatgc tcaacccttt catccgtttt ggggtaaaga aatacaaagt ggcagagccg
    42721 cttagtgagt ccatcccaag tgacgaaaaa gccattgatg tactgaataa gctggcctct
    42781 cgcgagctaa cggggaacgc agcaatagca gctgttgagt ctatcgtggc gtcaatgtgc
    42841 gccgatgggc aggacgtgtt ccgtcgtttc ctcttaaaag acccgaaagc aggtgttggg
    42901 attagcctat gcaacaaggt ttttgaaaat cccattccga aattcgaggt gcagctggcg
    42961 tcaccgtata aagaaaaagg cgacaaatac cccttcaagc caaatcctaa agcaaaatgg
    43021 ccgatgattg gcagtcttaa gctcgatggt ttgcgagtaa tttgcgaggt tattgttgac
    43081 gaggaagagg ttaacttcct ttctcgtact ggtaatccaa tcacgtctct cgatcaccta
    43141 aagccagcaa tgctagaatt aggcaaactt tcaggccaca aacacatctt cttcgatggt
    43201 gaaggaactg ccggttcatt taaccagtcc gtatctgcat tgcgcaaaaa gaacgtgcag
    43261 gcaattggcg ctatttatca tgttttcgac ttcttcctac cggaatggcg agcacaggct
    43321 aaatccaaag agtatgcaaa gacaggtatg aagctgaaag agcgcctggc tatgctggtg
    43381 gcgttgttta aaaacgatcg cagtgaaggc tacacacaag acattcacct gcatccgttc
    43441 tatatcatcc atagccacga agacttcatc gaacgcttca tgaaacgcct ggacgataac
    43501 gaagaagggg agatgggcaa agatccgaac tctgtttacg agtttaaacg tacccgcagc
    43561 tggtggaagt taaaagacga agattcagaa gatggtgaaa ttatcgattt tgagccgggc
    43621 gacccggact ctggttttgc caacacgctt ggaaaaatag ttattcgtct tgaaaacggc
    43681 gtcatcgttc gtgcgagcgg cattaagcat aaatatctgg acgagatctg gaacaacaaa
    43741 gagaagtacc gtggtcgtat tgtcgaggtt cattgtcacg agaaaacacc tgatggcagc
    43801 ttacgccacc cacgactgaa atggccgcgt tgcttacgcg ataccgaaga tcgaatcgga
    43861 gataaagaat gatcgtatta agtaaacggg agaaagaaac gcttcatgaa ataagtaagt
    43921 ggccggagtt ccctcagtac tggaagccta aaacgcgagc taatttagag cgtttagggt
    43981 tggttgcaaa catttcggaa acgtggtgct cagccaacta ccagttaact gataaaggga
    44041 aagtattgct acagcaatta gtagaatcag gagtgttaaa atgattccat acatctcatt
    44101 agcttttatt ggtggcttcc ttatcggctt cggcatctgt cgtgatttaa ttaagcagga
    44161 acttaaaacc aaaacactgt gcatcggaaa gcgtgtgtat cgggtagttc atgaaacaaa
    44221 ggttagaaaa tgagcaattt aacttcttgg gactggtggt tggccaccta tttcttagcg
    44281 gtcggagtcg cattcgcctt ttacgtaggt cagttagtcg taaaactgtt gctgattaaa
    44341 tttgctagtc ataaacgtat cgatgatggt ctgtggcgtc ttggcaccct ggtggaaact
    44401 cgctacgggc aacttaagga gaacgaaacc attactatcc aagcgaaacg attcactgcc
    44461 accatcacaa gaacacctag tcgtagagtg gccttgatca aaaaagtcac aaccgaataa
    44521 aaacatatcg ataagtattt acttacttat cctttgtgta taagatgact ttgttttcgt
    44581 tgagatgcga ctgtttgaac ttaaatacaa gtgcaaacga agaagtctat ctggcagtag
    44641 cctaataagc caaacaccag cgaggtcagt ttccagcctc gttaccgaaa tgggacacac
    44701 tgagcgagtg tgattgcaga acgcaggagg gaacactcat gttccctccg atgaagtaac
    44761 agaatgggcg gtcggtatat tttcaactcc atatgactcc cggattctta gccactgacc
    44821 gcccattctg ttacgtcatt ttgttcaatt atgtcgttta tactgggtta aaaagcggcg
    44881 acgtagcccg gctggtatgg ttagccagca cacaacgttg aggccattac atttttatca
    44941 attctaaggt tctattcaca gagataccgg cgagcgttga tatgtaacat gttgggcaaa
    45001 cattcaatcg gagtagtggc ctcaacgttg tgaagacagg attgttgtgt aggtttaacc
    45061 acttttccac aacaaatgat tccatacatc acattgtata aattacaaag taggtgctgt
    45121 cctcagaaac atcatctact taaagatttt gccttctatt gagagaagtc gaaagcgtct
    45181 gacactaacg aaaagtgcaa gtagcggtgc gtttcctggc agaaactaac accgccgcga
    45241 ttggcactgt tgagtaataa atactggcag tgccgaattg atggtgtagc tcagcggtag
    45301 agcagttggc tgttaaccaa ttggtcggtg gttcgaatcc acccaccatc gccaatttag
    45361 gggagttagt ccgtagaggt agcggggtag actgtaaatc tactgtcatt gcgactcggg
    45421 tggttcgact ctatcactcc ccaccaaatt gccgatttag ctcagttggt agagcagtcg
    45481 ctttgtaagc gaatggtcat cggttcgatc ccgttaatcg gcaccaacat aatattgaga
    45541 acattggcgt aacggggtca tatcccaatc tatgaataaa tgttgcgttg ccgcgtgaca
    45601 accagtgttc tcaacattgt ggtgaatgca caggctgatg tgccgcaact acagtagtgc
    45661 gcgctttgcg gggcttgcta caaccctgtg tcggagatca gcgccgacca tcacaatttg
    45721 gctctcttgc aacagcataa cgctgaaata tgtccaacct ggtgcggcct gttcacccgc
    45781 cgttagctcc acgaaacgga gcgcacaaca ggtaagaaca ttttcagagt tcgactctct
    45841 gagttgccat tcgtggtggc acgcgagacg tgaatgctct ttccgttgtg gttgatgcgc
    45901 agactgatgc gcggaaagaa atgccccagc gacacacgag gcgagttcac acactgcaat
    45961 gcagtcagta gccggggtcg ctaagccgga gatcagtacc ggtaaccaca acgactggag
    46021 agtagggagc atggtgctca agcggtcttg aaaaccgtcc cattgcgcaa gcgatgatgg
    46081 ttcgattcca ttactctccg ccagacacag cgttgagcgg tttggccttt taatcaactc
    46141 gattaagact ccgctaacat aaaccagacc gctcaacgct gtgtgtgaaa ttccacatat
    46201 tttacacata tccatttacg caatttttat tgttctttca tacattactc ttatatttta
    46261 ttgtcgtttt ttaactgcta aaatcgatac gattttagct aaagtttatc gttaaaggag
    46321 taacgattat gtcattcatt gactatgata ttttgtcagc atcatcaagc aaagagctta
    46381 gagaaaaact tgcggagaaa atcgcagagg gttggcaacc tttcgagtca ccaaacattg
    46441 ttgaaccaag taagggcgga ccaaatgcgt acaatcaagc aattgtgcga tctggtgatt
    46501 tatcacaatt gcccacaccg gtatcaaatg aagatgaaga gcctgactat tattatgtag
    46561 ttactatcgc aggtcaatcc aactcaatgt catacggcga aggactgcct atgccaaaaa
    46621 cttttgatcg gccacatcct cgcgtaaaac agttagctcg acgcagcaat attaaaccag
    46681 gtggttttgc ctgtaagtac aatgaaataa ttcccgctga tcactgtctt catgatgtac
    46741 aagacatgac taaattcaag catccaaaag caaacgactc tgaatatgga tgtgttggtc
    46801 agggactaca cattggcaaa aaactattac cattcattcc agcaaacgca ggtattttgc
    46861 tcgttccttg cggaagaggt ggttctggtt tcactgttac aggctctgaa ggccaatatt
    46921 atgaagagtc aggtgcaatc gaatcatctt taaaatgggg attaaatact cctttgtata
    46981 aggatattgt agccagaaca aaagcggcct taagtaaaaa caaaaagaat gtatttcttg
    47041 gtgttgtctg gatgcaaggc gaagctgatt tggcaactgg caagtactcg cagcataacg
    47101 aactgttcct caagatggtc gaaggtttca gaaaagagct atctcaatac gacgcacaat
    47161 gtgttttcgg aagtgctgca aatgtaccct ggatttgtgg cgatacgacc cactggtgga
    47221 aaaacaaata tccaaaacag tacgaaattg tttatggtgg ttataaggga aaaacctcac
    47281 aagcaattta tttcgttcca ttcatgaaag atgagaatga taataacgta ttaaccaata
    47341 gccctacaga tgatccagac atcacatcta ttggctatta tggttccggt catcgcaagg
    47401 ataataaaac atggacttcc gagagtagag agagtcattt tggttcatgg cagcgacgcg
    47461 gaataatatc tgatcgtctc gcaactgcgc tgcttgcaca ttcagcgaaa tcatctgctt
    47521 ttgtgaatgg cgttacaggt gagattatta aacaagaaca acctactcaa cctaaaaatg
    47581 acaacattgt cacaactcca accccgcgaa cggttatttc cttaactgga gctggtggtg
    47641 atgtcgctac tgaacaagga tggactgtaa ctggtgcaaa tattcagtca gttgaaaacg
    47701 gaaaagcaat tgctttaact aaagcggttt ccaacgaaac ttggacgatc agtaaagcta
    47761 ctgacatagc acaggctctt cttgagaatg gaggtaaatt actttgtaag tttaaattat
    47821 ctggaaaccc tgatccaaaa aaagctaagt ttggctttgg tatatattac aaaaccagta
    47881 aacctgttgc tgacgatata tcattcgacg gtactgggag cctgtttctg gctaacttct
    47941 tccttcaaac aattggaggc aagttgaaca ttatgatgca caggacaggc aataatcgaa
    48001 atgttcagat agactcatat ggtgactacg acaacaattg gcacactctt gagttgcaat
    48061 atgagaaagg cacaggaact gtaattcccg ttctcgatgg caaggcgggg agtaaattca
    48121 agctggttaa agacaatatt gctcttggtg atgaggcttt tgttttaaca gatataacaa
    48181 acaaccaaac tcatgatctt gctatcgaaa cacttaccat tagcatcaac aacccaacct
    48241 aacacaaagt aacagccagg catgaaattg cctggcttca tatcgaatca aatatttaaa
    48301 actagctcta cagaaaaaaa tgattccata ctctttaggt atggaatcat tttccgcatg
    48361 aaagatttta gcttgattcc aaaagaatca atgcctaata tactggttac ttatacaggt
    48421 gatcagcgta actaggtgaa tgccaataaa tgaaaaatag ctttgacaga gcacgcgcag
    48481 cagaaaacac ctcaaaagag gcaattgagt atctcgaaag agcatctcaa atgcaggcca
    48541 ttatgatctc acagatcaac aataacatga tattctcgga cgcattcatg ttattcactc
    48601 gcttatctct gctgataacc agacgtcggc cagagatcgc tgttcattgt attttgatac
    48661 atgttttgcc gcacattgct gatgtaaaag taagtgacat taataggttc atggttaacc
    48721 aactggtcaa ccctctaata ttggatggca aaattgttat gggccgccgc gttttctctc
    48781 tgatgaagca gttccttagc tggtgcgcct tccaggggat gatagacgtg tcaccgttaa
    48841 acgatatgtc actaaacaaa gttgccggtg gcgcaaagcc cacacctcgc gagcggaagc
    48901 tgaccgacgc agaggtatgg gtgttctgga atatatggga ctacttcaat gtgtgcgctg
    48961 gtacaaaatg ggcggccagg ctatgtcttg tatcagcaag acgacctgac gaagtactga
    49021 gggctaaaaa aagtgagttc aatcttaagc gtggagtttg gaatcaaggc aagagaaaca
    49081 agtcagcacg tgagcattcg ctacctttaa gcacattaat gcgcacatgc attgaagagt
    49141 tgttcgaata cggtaaagac agccagtggc tcgtgccttc gaataaaaaa atcgggaaag
    49201 accttcctat gtctaaagtg gcaatagccc aggcattacg tcgtattctg gaacgaccag
    49261 aactgatgga gcttgcgcca tttacacccc gagatttgcg ccgtactgcg cgtagttact
    49321 tcccagcatt aggcataagc caggaggtat cacgcaaaat aatgaaccac agtcttgagg
    49381 ggatagatcg ggtctacgac cggcacgatt atatggacga gatgcgagac gccttagaaa
    49441 gtttctcaac gtacatcgca tcaatcgtag agcaaccgga tttagacgaa attgaccaca
    49501 aattcaaggg agatcgtcta tcaacagagc ttattcgtct aaatttttca tagagacttt
    49561 atggcctcaa caaccttttg tgatgcgcct ttctctttac cgaatcgctc gttatatgca
    49621 gcaagaacct gtttttcgtc ctcgttaaga ggagcggtac cttctttgta taaaaatgct
    49681 gcgagttcag gttggcgttc ttccagcacc atcatcataa gacgacttgg ctcaataccc
    49741 agcgccaacg ccagcggacg aaccttatcg ataggcaaag gaattttacc acttttaatt
    49801 aaagaaaggt tgttggcatt tttataacca ataagtctgg ctatttgggc ctgactcata
    49861 ggtgaggatt caatcagccc tgcgataaaa gcagcatagc gactttctat aaattcaatg
    49921 tttttgtcag acattattgc gacctttgcg cgttaaactc tctggtaagt gcttaccgat
    49981 attacatcaa aggttagggt tgtaaagata taatcatttt ttctacaggc acaaatatta
    50041 accaatcagc attcattaat ggagaaaaac gactaataac atgtaacaaa actaactcag
    50101 atatgatata gataagttca attatgatat aagatttaat agtactccct accatatttt
    50161 aagttagaat ggattgattg aatgaaaaca actatttcca gcctaatcac tcttgagatc
    50221 ggacacgttg aaaaattagc tgatgaatgt gtagctgaca ttctcaccga attaccaaat
    50281 gagcagattc aggttggtat gcatgacgca acagccttta tattcctact taataacaaa
    50341 cgcttcacgc ttatcaatac cggctctggc tctttagccg tcagaacctg ttaacccctc
    50401 tgctacctgc gcgaatggct tagttccctg ttcgcgcagt gctacattaa acacactagt
    50461 aaataatttg ttttcataac aaaggattag ccatgtctaa aaaacgttcc atcaaagagg
    50521 ttcaggactt ccgtgacagt gtaaaacgag tagtcgctct cctttcaggt aaaaacatcc
    50581 ctgttgcaga acgaggagac gacgcttatg tacgctataa cgatgatgga gagccaattc
    50641 tcgtaaacat cccatcaatt ccggataacg caacaccggc attgatgaat gctgtgcgcg
    50701 gatttctcga tcatgaggtt gctcacattt tgtttaccga tattcgtgtc tccaacaaaa
    50761 tgagagaaaa aggacgcgtt ccttcccggt cactagggaa tgcattagaa gacgtgttca
    50821 tcgagcgaaa aatggggcag gtctttaacg gaacgagacg taatctgatg gcaactcagc
    50881 gccttataat cgaaaaagtc tttaaaccaa aggcttcaga ggctattgct tattgtggca
    50941 aagatcagcg cgcgcttttt ctaaacttct ttctctgtcc ggttgtaaga gcctgggatg
    51001 gccaagcacc gttcgtagat ttcatggatg aatactggcc tgtcattgag aaaccaattt
    51061 cattattaaa agaacatggt atcgatgtgg ccgtgcgtaa catgtcttgc accgaggatt
    51121 gtgtaaaggt ggctgcgacc atagctaaga tcctcaaaga cactgaaagt gaaagcaaag
    51181 gtaaggagtc agctccggga aaaacttccg atccttcaga cgctgaccag acggatgcct
    51241 ctggagaaaa caatgaagac aacgaagatc atgagacacc ctcagcgtta gataatcaca
    51301 aatctatcga atcagaatca cacagtaagc acaaacatga taataacgac agtgatgatt
    51361 cagataattc tgaatcatca gaaacaatat tcgatgatac agaaaatgat aaagaggtat
    51421 cagattctga tgcttctgat aacgcggcgt cagaatcatt aaccgctgac cacgaaaaaa
    51481 gaaaaacgac agaagacggc tcttcagata ttccaactcc gtcaaaaatg agtctggaag
    51541 aggctttaga ggagctggat agcatagaag atgaagtcgg aggcatgaca gaagatgctc
    51601 tatccgaaac gattaaaagc gagttaacag aaagctcgaa aagcgaatac aggccataca
    51661 atcgctcata cgacttcatc ggctcgattg atcaggcaga agcccatatc aaacggctta
    51721 ttaaaacatt ctccgatatt gatttaggag gatatccaat cagccgctat cgcatcgttc
    51781 ctgaaggcaa ccagctcttc gacaaatata ttgaaaagca tctttcgtca ggtgtttcgt
    51841 cgacgctggc aaaagacctg gagcgagcaa tagcaagcag aaacagagtt cagtttatac
    51901 cgggccagcg tcgggggcgc attcatggtt ctagtatcta cagattagca atgaatgatg
    51961 atcgcgtgtt tcgtaaaaaa gaagaatcta aagccgttaa cgcctgtgtt caacaagtga
    52021 ttgatttatc aggttcaatg agtggtataa cgatacaatt ggctcttgca agttcatata
    52081 ccatcgccga tgcccttgat cgaataaatg ttcccaacat tatcaccggc ttcactacat
    52141 ttggtagtca tatggcggca ggagaactta aggctgtcaa gtatgagttc tctcgctttg
    52201 aatctttaat gctacctatc atcaaaaatt ggaatgaaaa ggtaagttct cgcgaagttc
    52261 gctcacgtat ggggtgcgta ggctacacat tcccacttct taataacgtg gatggtgaaa
    52321 gcatagccag ccttgcatcg ttattttccg gtcgcatgga ggacaggaag atcatgcttg
    52381 ttctgagtga tggcgcgccg tgggctgttg ggagaggttt tgacgctcat ttgcgttcgg
    52441 ttgcgaagca aattgaaacg cagactgaca ttgatttgat ggcaattggc atcatgactg
    52501 acgcaccgga gagattttac tcaaatcatg ccctggtaac gagcgttgat agtcttggtt
    52561 catctgtagt tactgaacta tctcgtatca ttttgaagtg attagaatag ccttaatgat
    52621 aagtaatcac ttacgatatt tgatggtata tttatataag aagttgaaag ctcattagaa
    52681 aacaaaggaa aaacgcatga ctactactgc actgcaaaat gaaaaaaatc cttctgatta
    52741 ccttgtttgc aagtggtgcg gaaaatcatt tcactatttt aagtcccatg tagccaatgg
    52801 taattgcgag ggcattcctg agtcagtaaa agatgccgat cctgacaccg tactgaaaat
    52861 gtacacaaca cagtttccag atgagccaac gctatcgaaa aaggcacttg atgcaattca
    52921 agctaaacgt gccgagcaaa aaagcgaaat ggccaaatct tctggcttga ccagtagccc
    52981 tggctacaca ggcacagttg agtacaagac agatctggtc gcagctcacg aactgctaaa
    53041 cgtaacggtg gaagaactcg gaacaaaacg tgggacgccg ctcatggtta gcgtcaacgt
    53101 caatacgccg tatccagagt tcgttcccga agtgaagaaa ggctacgtat atggcgactt
    53161 cgaactgatc aaagatattt tcatgatgct tgaacttggc atacctggct atttgtgggg
    53221 tcatgcagga acaggcaaat cgtcattgcc tacacagcta tgtgctttgc tcaatcgtcc
    53281 gttgatccgt gcccaacata cagcatcaat ggaagaggca catgttacgg ggcaaattct
    53341 ggcgcgtgat ggctctacgt atttcgaacc tggcttgctt gcgctcgcaa tgaagcatgg
    53401 ctgggtttac ctcgcggatg aatacgactt tgcgtttcca caaattcttg gcgtgtatca
    53461 gccagtgctg gaaggtgaag cgttggtcat caaagaggca actccagaat ggcgtcgcat
    53521 tactccgcat gaacggtttg ctttcattgg cactggcaac acgaacggat ctggtgatga
    53581 aaccggcttg taccagggta caaacatcca gaacgccgcg aacttttcgc gttttggcat
    53641 cgtttcgaat gtgaaataca tgagcaaaga cgcagagatc aacatgttga caaatgcagg
    53701 catcgtggat gaatacgctg aaaagatggt taagtttgcc ggtatcgttc gcgatggata
    53761 cgaagaacac aatatcagcc aaccgattgg gcctcgtgag cttttgttgt cggccaagat
    53821 tggaatgatg cgaggcgatt ttgtgacagg tattgagcgt tctttcatta acaaactccc
    53881 ttcagcttct gcacaagcgg ctcgtgaagt tgttcaaaaa atatttggtt gatcgtgcgt
    53941 aaaggatgtt tcggctctct tatcgctgct tctgaaactg gtaaggcttg tctggagtgt
    54001 ccagacaagc cttattgtca ccaatcagca aaagaagttg cgatttcgat gcatgggaag
    54061 ttcgtaggct tcccaaatga caaaatcaaa aaaaccagaa aggtaaaaac acatgaaggc
    54121 actgatggtt cgaactgact tctcacttgg ggagtcggct ctaaaagcag aaaacgcggt
    54181 gaagattgcc agagaagctg gctacaccgc tgtaatttca gcagatagca tgaatattgc
    54241 gagtgttatt ccactacaac gtgccgctgg tgacgacatg gcggttattt gtggtgtgaa
    54301 actaaacatc gttgatgatc ccacatacga gcaccgggat aaacttgcta aagaatctaa
    54361 gggatgtatg gaatcattag agcgaggacg taactactcg tttaccgctc taattaaaaa
    54421 tgagcaagga tatcgcgaca tctgcgaact aatgacggtg gccaacacac gagaacagtt
    54481 ctactttgta ccgcgtctct cgctcgaaca gttggtttct acatatgcca aaggcaacat
    54541 catcctgctc acttccgaca tcggtagcgt gttccaacgc aacgattttg caaaaatcat
    54601 aagcacactg attacaccgg gtggaaaaga caacttctat agtgtggttt atccgcaccc
    54661 taccccattc tacgaccaga ttaacgtccg ggcgatgaaa gtcgccagcg cattgaaaat
    54721 agagccagtg gcgttctatc ccgcttatta cgaatcgatc gacgatgcag acattaaaga
    54781 cattgcgcac atggttacga acaacataaa aatcgaccag ccgcatcgtc tgcgtatccc
    54841 ccaccagcga gataacgccg tcaatggtcg ccgccatctc cttgaggcgc ttaaagcctt
    54901 ctccgttcgc atggatgtgc cggtaacagc tgcaatggcc tcaacaacgc aggataccat
    54961 tatcgatgcc tgcacatggc gctggcatga attgccacca gcactgccca agatggcaga
    55021 cgacgaacct gcaacgctga tgaaactggc tgttgcaggg ctgcgtaaac gtcttaccac
    55081 aaaagagttt ggatacacac cacctgcttc tgagaacagg gtttatgttg agcggctaaa
    55141 gtacgaaatg gacacgctga ctcgcctggg attctgtggt tacttcctga tggtgcgcga
    55201 tctgatgaat catagtcgtg aaactggcat tcctgtcggg cctggtcgtg gttcctccgc
    55261 tggatctctg gtggcatggt gcataggcat aaccaacgtc gacccaatcc gtcacggtct
    55321 tctgtttgaa cgtttcatca accctgagcg tctcgacttg ccggatgcgg atttggactt
    55381 cagccaggca cgtcgccatg aagtgatcga gtatctgaat gaacgctacg gcgaagatta
    55441 cgttgcaggc attccgaact tcacctacct gggcgcagcc tctgcactac gtgataccgc
    55501 tcgtatttat ggtgtggagt ccgcagatat ggcggtatca aaagaactga agaacgtcga
    55561 ggatgatagc cttccattgg aagagctgcg cgaacaactg gcaagtctcg acaaatacgc
    55621 aacaaaatat cctgatgcat tcaatgcagc ctgcaagtta caaagcctta tgcgtggctt
    55681 tggtagacat gcggcaggga tgatcgtagc aggtgttcct ctgacagaac gtacaccggt
    55741 tgagcgccgt ggtgacgcgc gttgtatcgc atttgacaag cgttactgcg aggctatggg
    55801 cctaattaag ctggacgtgc ttggcctggc aactctcgat ttgctcgata gtgcaaaacg
    55861 ctacattaaa gagaacacag gtgaagatat caatcttgat accatttctc ttgaagatcg
    55921 caaggtgctg gatggttttg ctgctgggta cactcaaggt gttttccagc ttgaatcagg
    55981 cccaatgcgc aagctgctta aagatctggg cggtggcatt gagccaatga gctttaaaac
    56041 cgttgtcgcc acgactgcgc tcttccggcc gggaccaatt cagtcaggca tgttggatga
    56101 ttatgtttct gtcgccaaag gctttatgac gccggaatca ttacaccccg ttcttgatga
    56161 acttaccgca gaaacaaatg gcgtgattct ctatcaggaa cagacgatga acgcgactcg
    56221 attgcttgct ggcttcacaa tggctgaagc tgacggcgtt cgtaaagcga taggcaaaaa
    56281 agacatggaa aaaatgaaga gcatgggcga gaagttcatc gttcaggctc aagctggctg
    56341 gatagacgtt gagctggaag atggcactac acagcgcatt caccgtgcgg aacattttaa
    56401 atgcgaagac ggaactatga aaactgtcga agaggcactt gagtacggcg caaaactacc
    56461 tataaacgca gtacgcgtta cagcgtcaca tccagggcta tcagagatga aagcgaagga
    56521 gatctggacc gcattcgaaa agaatggtgc ctaccagttc aacaaatcac actctgttgc
    56581 ttactcctta atcagttatc aatctatgtg gttgaaaact cattatcccg cagagttttt
    56641 cgctgctgct ctcactattc ttggcgaaga taaacaccag gggctggtta aagatgcgct
    56701 gacctatggt attcgcgtat tgccaccaga cgttaacgtg tcatctaacc gaattgagat
    56761 ccgcacgctt gaagatggca gccaggtgct gtatgcgccc ttctctgctg tgaaaggttg
    56821 ttctgagaat ggctgccagg ccatcatgag agcgcgagaa aaagttggcg gcaaattcga
    56881 gtcactggcg caattcgaag aagctgtcga gaaacgtgcc tgtaatagtc gagtgcgcga
    56941 atcactgcaa aaagtagggg cgtttgcatc catcgagcca ggcagtctgc cagcaactga
    57001 tccagaacgt ctccgcgacc aagcggaatt gatgggaaat cttgtcatag acgcagttaa
    57061 agcctcacgt ccgtttgaga tgaactccaa acgttcggct gaaattaacg tacttatgac
    57121 gcggatggcg gctgaaatgg gcttaggtga tgaactaatc cgccccagca ttggtattaa
    57181 gccgaaaatc atgatcattc tggacaatgc aaacggcaat gatgctcgaa ccggttactt
    57241 catggagaac ggatacgacg attttaaggc caagctgttg acggctggag atttgcgcat
    57301 gggcgatctc tatgtcacag gcgtttgcaa aaaggtgaaa gacaaagaaa aggactacac
    57361 caaagacgag atcggccagt tcaccgactt tatgcgtgaa gagatcaatc tggtacgtcc
    57421 gacctatgtg ctgacgtgtg gcagtcgggc aacggcattg tttaacaaca aaaacaagcc
    57481 atccgattta gtcggtagga aggagtatct tccagacctt gacgttacag tcttttacgg
    57541 gtttaacccc aacattcttt acttcagacc tgaagaggga gaacgactag aagcgatatt
    57601 agccgacgta gcagaaacta ttaagtcgtg atccaataaa catggcctct atggccatgt
    57661 tttctcttat cccctcatat cccctatcct cgaactcctt gccttatcat cacaatgata
    57721 taatcaatat aagatgataa gtaaaaagga aagcacatga tcaccgatat ttacgagaaa
    57781 ataatgtctg atctcgagtt tgaccgagac aatctggagg aagtctggcg tagacaaccc
    57841 cgccttttaa tggagtatgg ctcaaaactc gctcatgcag aaagaagtgt cgcagaggca
    57901 aaacttaacc ttgaagctgt tgaagcaaag ctatacgaca cagagcgtaa gaacttgagt
    57961 atgaacggca ttaagttcaa cgagtctgta ctggacgcta aggttaaaac gaacccacag
    58021 tatctatcta aacgtcagaa gttggatgac gcacggcaca tagcagacat atacaaacat
    58081 gctgtcgccg ccttttcgca tcgccgagac atgatcgttc aggcgtcaaa gatggccatc
    58141 gttgaattag agcgattagg ctctgaacgc tttattactc cccgttgatt tttgatagat
    58201 aataagtaag tactgatcta tcatttaaca gctcgaaaga gccacgaatg aacgaaagcc
    58261 caacgcgcat agcgccatcg gccaaatcac aacaaggaga aacacatgtc taagacatta
    58321 cttgatttgc ttaacaaaac tcgtgaagac attgccgcca aacgtggcaa caacgttgat
    58381 ctgactcgct taaaagacgg cgtcaactat atccgcatct tcccgaataa agacgaccca
    58441 aacggtaagt tcttccagac tttcggtatg cactacgtta agtatcagaa cgaggaaggt
    58501 aaagaagcaa ccaacgctta tatttgtgag caacatactc acggtcgcgc ttgtcagcta
    58561 tgcgaaatgg tgatggaagg tcgcgctcgt cacaagggta acaaagcaat ggaagaacgc
    58621 atcggtcaaa tgcgtgccac tcctcgctac ctggtcaacg gcattctttc tgctcgtgag
    58681 gatttcgcag atgctgagaa atgccaatta atcgagctgc cgtctactgt attcgatgat
    58741 atctgcaaag caatcaccga agacatcgct gatgatatcg gcaatccact gagcaaagag
    58801 gaaggctacg cattcctgat taaacgtact ggctctggtc gcgataccaa atatgacgtc
    58861 tcgcctaagc gtaaagtcta caaaggcgat atcgaagata aattctggaa cacccagcat
    58921 gatctgatcg catacgcaaa tcaggctgat gaaactcgtc ttctgtcgac agttcgcact
    58981 atgggtcgtc tgattggcat cgctgcacca actgccgcag catctgcacc agcaatttcc
    59041 tcaaccgcga aaacatcggc tgcggcacta cctggatttg gctctgtcac tggtcataca
    59101 gaaggagcga cggctgtagc aaccgcgcac acaccggctt ctgaaccaac cagtctggtt
    59161 gatgaagaaa tcctccgtgc cgttgaaact gaatttaaac cagaggcaag ttccgctgcc
    59221 gttgccgtat cagtcaaaga gtctgaagca gtcgcagcga catctgtagc agccgcatct
    59281 gcgacggaag atgaaggtct ggatgaccta ctgagagagc tggactctct gtaatcccat
    59341 tacgtgacca gtaaggcgtc tacggacgcc ttactttttg gaaggaatgt accggtgaat
    59401 tatctcttcg tagatggcaa tagcctgggt tattaccacc aacaatctga caaattgcac
    59461 aacggcgaaa tggaagtaca ggctgctttc ggctttgtta agaacgtccg tcgttatgcc
    59521 tccatcctcc atgcccgacc tatgattctt tgggatggat ttagtgacaa gcgtcgcgac
    59581 ttttacccgg actacaaagc aaatcgcgac gacgatcctg atatgaaaaa gatgaaggaa
    59641 ggctttgcta tccagaagcc atacatcctc aaaatgatga ccgcgcttgg agttacccaa
    59701 ctcattgcaa aagatgcaga agcggatgat ctggccgggc tgctggtagc cagcattgca
    59761 ccgcagccaa ttgttgaaca catctatctg ttaacaggcg atagcgactg gcttcagtta
    59821 gttcgcgaaa acgtaagctg ggtaagcctg cgcgaagacg ccaaaaacaa gcaggttaat
    59881 tttgagcaat ttgcggagct gacaggattc gctactcctc gcgcattttt ggaagcaaaa
    59941 gcattacaag gcgataaatc ggacaacatt agcggtgttg gtggcattgg tgctggcggt
    60001 gcgaaagagc tgctgcatga atggggaagt gtcgcaacga tggtacgcgg catcaacgac
    60061 ggctcaatcg tggttgacaa aggacgccat aagaccgcct tcaacaaact agcgaagaat
    60121 gccttcaacg agaaaacagg ctgtcgaatg ctcgaagcgt tcaagagaaa catcacgcta
    60181 atgaacctga ttgagacgaa gtttccgcct accgaaatcg aaacaatcaa aggcaatcgt
    60241 gacgtgaaag cattcgagca actgtgctac gagctgaatt tccgttcgtt ccttgaagac
    60301 cttgaagtgt ttgttcttcc attcgaaagg tattgctaat gcttaaatcg attattaatg
    60361 gcgctacaac cacccctacc caactggcaa aagagattgt cttttatcac ggtgagtacg
    60421 ctgtcatcgc actgccgtca attctaggcg ctgccggaat gaaagcgaca gatcgcgagt
    60481 ttggattagt cagcgagcag gtcgtaaaaa tcctcgctcg tgtatccaga ctccttaacc
    60541 acgatgcgat tgtattcgac gaatccgccg ctttaaaacg aatcaacgaa acaaaaggag
    60601 cctgatcatg gcaaaaggaa aatccgcact ggcacttgct ctgaaaaaga aaatcggtag
    60661 caacgacgaa attcagaaag taactcattg gattgacaca ggctttcctc cgttaaacaa
    60721 agcgatttcc ggtcgttacg atggcggttt cccatgtggt cgtatcgtag aagtattcgg
    60781 gccaccaagc gcggggaaaa cctttttggc aacggcagca atgatctccg cacagaaaca
    60841 agatggtctg gcggtattcc tcgatcatga gaacagtttt gacgttggcc tggctgtagc
    60901 caatggtttg aatgcagatg aggatgacgg tcagtgggta tacaagcagc ctgatacctt
    60961 cgaagactct gtagagttga tcggcacaat tcttaaattg gtacgtgatg aagagcttat
    61021 tcctgaatca gcacctatct gtatcgtggc tgactcactt gcgtctatgg ttccgaactc
    61081 caaagccgag aagttcgaaa agatggctga aggcactgcc aaagacaaag atcagctaaa
    61141 catgaacgac aatacggcac tggctcgtgc gacaagtgcg aacttcccta ctctggcttt
    61201 gtgggcacgc aaatacaacg cctgcatcat cttcttgaat caggttcgca caaaaatcgg
    61261 tgtaatgttt ggcgacccta ctacgtctcc aggtggagat tcaccgaagt tctacgcttc
    61321 tgtacgtatc cgtcttggtg catcggtgat gaaggatggt aaagagaaga tcggccagga
    61381 cgtaggcgca gaatgcatca aaaacaaagt tgcaccaccg tatggcaaat gcacctggaa
    61441 attctacttc gatcctactc gtggcctcga cgttatcgaa tcgctcgtcg agtacatgct
    61501 ggaagaagga tacctgccaa agaacgccag cgggcgagtt gaaattggtg acaagaaata
    61561 caccaaatcg cagatcgtcg agatgtatcg ggagaagcca ctggctgaaa tcattgcggc
    61621 tttgcaggca atcgacgacc gaagagcaaa agacaacccc accgagtcag tagaagagta
    61681 aacacaaggc gtccacagga cgcctttttt atctcttgaa aatatataag tacttactta
    61741 ttattttcgc ataacaacca cataggaaaa cacatgatca aaatctatct attggcagta
    61801 gccacaggcg tttcagtggc tctcatctac ggtttactgg ttccgtcgct gatttctacc
    61861 aagagtgatt tagccgtcat gtttggagtt atcgttggtt ttggtgctcc tgtaatcggt
    61921 cttattgctg gtcgtaagtt tatcaactca ttaatcaaag caaaggggaa ataagtaatg
    61981 aagaaaggtt tacttgcagt tgctctggcg gctatttgca caatgggtct tactggctgt
    62041 gatcgcgtgg agcctggata cgttggcatc aaagtaaaca aattaggtga agacaaaggg
    62101 atcggtgaag tggttggcgt tggtcgccaa tggacaggtc ttaacaccga actttacgta
    62161 ttcccgacct tcaaacaaat gaagacctac gacgaaccgt tcacattcca gatgagtgac
    62221 ggtactgcta ttggtcacaa aattggcgtt gcgtatctgg ttaatcgtga caaggtaacg
    62281 acggtgttcc agacctatcg caaaggcgta gacgatatca ccgaatcaga tctgcgtcag
    62341 aaaattgccg actctctaaa ccgtttggcc agccgtatga ccactgactc atttatcgac
    62401 ggtggtaagg cgcaattgct ggacaacgca ctgaaagata ttcagaaaga gatgtctccg
    62461 gttggtattg aggtactgag cctgtcatgg gttggaaagc ctgattaccc aaaaaccgtc
    62521 attgaatcta tcaacgccaa agtaacggct aaccagcgta ctctgcaacg tcagcaggaa
    62581 gttgaacaac gtaaagctga ggcgaatatg ctacgtgaac aggctaatgg tgaagctgat
    62641 gctatccgtg ctcgtgcgca agcagaagca gacgccattc gtctgcgcgg tgaagctctg
    62701 cgtcaaaacc cgaacgttat ggaactggaa gccatcaata aatggaatgg ccagttaccg
    62761 cagtacatga ctcaaggggc taacactcct ttcattacag tgaaataact ctcattaaga
    62821 agatcaggcg tccagttgga cgccgtttta ttctcgttat tatcacacac aagaaaacaa
    62881 tttgttaaaa ggttaagtta attatgccca accacattac aaacgaaatc cgagtcatag
    62941 gtggtacaaa taaacaacgc ttagatttca tacgtgccat aactaacaaa aaaggtctaa
    63001 ttgactttaa taccatttgc agacgtccga aaagtataga aatggaagaa aacaatcata
    63061 tcaggcgcat ggcctctgca atggcgggcg aaacagttta cgaccacatg ttcggtgaat
    63121 taaagactcc agaacaggtc gaattagcta tgcgtgaatc aggaatgaca cgcaaggaaa
    63181 ttcgcaaaat caaagagcag gctcttatgc ggcttgagaa ttatcgtcgc tatggctatt
    63241 actcatggtt tgactggtcg cgagctaatt ggggaacaaa atggaatgcc tacaacatcg
    63301 aaatgcccgt aaaacgtatc cctacacgta ttaagtacgg ccataatttc cgccatacac
    63361 acgttcgagc atatgcaaaa cgaatctata aaaaacgtct tgcaagacat gccgcaactg
    63421 gtggtgagtt ggtgattcgc tttgaaacag cctggtctat gccaaaacca atttatcaag
    63481 cgatggctag aaagttcccg caccttgagt ttgtaatacg ttatgcagac gaagacttcg
    63541 gcaataactg cggattcatt ttacttcgca acggcaaatg ggaatatgac tacattgcac
    63601 cggcgaataa caaacaatcg tcagaagaaa aaatcaaatg gcgcaagttc gcctttgagt
    63661 tatgttgtcc tggtatcact ccacaagaat acggtctaaa tgaagaatac gaatacgccg
    63721 gatattaaaa aaaattgtca tttagcttta cgcatgaagc atagaggtta ataagatgaa
    63781 aagaaaaatt gaagaaccaa aaacgaccct taataaacat ggggaaaaaa ttcaaacaca
    63841 tccagcgttc ggtcttgtga aaacaagccg tgttagcaca actggcattc gcctatttga
    63901 ttcagaactg gaacatagag actatattga aataggcgtt tacgaggcgg aactgtcctt
    63961 agacaatgaa cgaacccgcc cacaaagaag ttgcaataga cggcctttag ttgaaattag
    64021 acttagccag gcacaatggg ctgctatggt tagcagtttt ggagtgggcg aaggtgtgcc
    64081 atgcactatt tcatataaaa ccattggaga ctcaattaga gttcatgata ttgccgagca
    64141 aaaaagtatt cggcaaaaat tcaacgatca aataaaggcc gctacgaata gtgagattga
    64201 tcgacttaat cagcaagttt cagagctagg tgaacttgtg aaaaaaggaa gagctggaaa
    64261 acgtgacctt gaagaactat atagaaaact agcatctgct gtagctaacc ttcctgaaaa
    64321 tctgtgcttt gctaccgaat taatgcagga acaaatggat gaaattgtct catctggaaa
    64381 ggcggagatt gaggcgtatg ttgcgggcac ggttatgcgt aaagggttag cagcattagg
    64441 cacagaatca tgcattaacg atgatcctac tgtctctatc accaaaatgg aaagcggtaa
    64501 agaattactg ccataaatgc atctgaggta ctgacaatgc ttttaattca acctgggttc
    64561 ggtttaaaga taaaaaaagg gcatatgttt ggtgagcgag attccaaacg aaaaattctg
    64621 tctattcgat taccatttat caccattcat tggttaaaca gagagtcaac cgattattgg
    64681 tataaatgcg caagagcagc atttaacgac cctgactggt ttgtagaaaa tcaccacgcg
    64741 gttcgtcagg caaaaagaaa ggctaatacg acatatatga aggcatatca ggatgcctgg
    64801 gaagaacatc gtggccgata ccaaaaaaac attgaaaaac ttgaatcaga aaatatggac
    64861 ctaagaagaa aactcggtga agcacgtcga gacatcgaag catataaacg tcttgtatcg
    64921 gagtgaatat atctttgctt tattttttca aaggtgatta ttttcctatt tataaggaga
    64981 agtaaacatg gcagcaatta agaaactcta cgatgccgca aacgtggctc tggatgttat
    65041 tgatgatgaa gtagcaaaag gctttcctga acctgattgg gcgcatcagc tacgaaacgc
    65101 tatcgcagaa atgaccccac cagatccaac ccccgacgag acagactggc agcgattcat
    65161 ccgtatgtac gctcaggaaa taggtccaac gccaacggca gagcaggcaa tgctgctgaa
    65221 atacttcaaa gaggcgggag aggatttacc aattgatgac tcagcatatt ggttccactg
    65281 cgcatggcgt aagtatgacg tgatattcac acaaggcatg ggaagcaaag atatggttgt
    65341 gtggcatcta ctccatatag acacagccgt tgacagagtt attgaacagt ttttccctaa
    65401 ccaagaagat tgatcgccta ttcataacta acaaaataag taaacactaa ccacaaaagg
    65461 aaaaacacat gagagtttta gttcgaatcg ttaccagcac tgtctatgac gtgtttccgc
    65521 tttttatggt caaagtcgat ggccttaacg atgaagaaac tgacgcgctg atccagcgta
    65581 ctcttgttga atatacaggt catgacgctg attcagtgat ggttgatgat gatggtgttt
    65641 gttggcataa cggcaactgt tggtacgtag aagagactca acaaatcagt aatgaagatg
    65701 ctgaacatct tgagcgtatt ttaagcatca gcacttttga gtgagtttac agtaaagttt
    65761 atataagtta gtatctacct atcatgaaga tttttattga atacttgtta ctcatcgttt
    65821 caatagcttt tgtcatcgac tgcattttca ctggtgtcat tcgtaaagtc ttttccccgg
    65881 tgcacgacgt agtcataaac gctttggcta tcgtgctcgt atttaattca gcatttgatg
    65941 taatcaaaga ggtggcagca tgaagaccat cccattcgcg ctgttgtttc tttcttcgat
    66001 cgttgtggcc gacaccactg tttatcagtg tgaaatgtct gtagccgacg ttaagaatgg
    66061 cgctcttacc gacgtcataa aagcaccata tggagcgatg gtcgtagaca gcggcgacca
    66121 gttctatgtt gtgcgtgacg atcgagtgtt gtcatcccca tatctcacaa atcgtaatgg
    66181 caaattaacc ggcgtcggag aagaccactt cgtatacaac aaatacaagg gcttctatgg
    66241 cgttcacgct tctcagcaaa gctacctttt cgatgactgc aaggaggttg ggtaatggca
    66301 ttaacactgg caggtctgga aatcgagaaa acaagcggct actggcgtgc taagggtttc
    66361 aagcagcctg gcattcttga gcgtctggaa cgtgaagatg ggtatatcgt ccaccagcgg
    66421 cgtgaatggc gtatgtacga tccagaaaca ggaaaactga ctacaaaagc cggtacactt
    66481 tggggtctgt taaagaaaat acactaaatg caaactgact gcggcacgtt ccgcagtcat
    66541 atttcatagt cgtcaccgct gacagcatac acaatcaact accgctgata gcatatcgag
    66601 agtctatctc accgctcaca gcatacttta ctcgattttt taccgctgac agcatactta
    66661 agacattgca tgaataatgt gtaccggtat gggtataacc agaacaaaat taccgctggc
    66721 agcatacgaa tgtctgacat atgccattaa ttaccgctga tagcatatcc aaacaaaaat
    66781 tcctcaataa aacaccgctg acagcatacg ttctatcagg gagtagcagg caataaatgc
    66841 ctttcactac aagcaatcag cgcaatagca atagaatgtt agtgagcgca aacctatatg
    66901 gaatgcactc ttcgaggtta gtaaccactg gggaggtatg acagagcatt gagtggtgat
    66961 agatgattta ccgctcacag catacgttca tctcactata ccgctggtag catatcttta
    67021 accgttcaca gcatactttt cagaaaaata gccgctgata gcatacattt caccgctgac
    67081 agcatatcaa agcagtttga gactattgga aaggatctca atcatcttga tattttcagg
    67141 cgtcaaattc tgcgaaagtt cggttatctt gttgataatg ttctgtttgg catcaatttc
    67201 cccagctttc tcatctggtt ttttgggttc gatgtcttca ggttttggcg gtgcgacttt
    67261 gagttttgga ttgcggctgt gaatctggat atagatcgac cgcccacgct taatctcgct
    67321 gtattcgaga tagcccaaat cttggagagc ttttaagccg ttacgtatag tctgattctg
    67381 cgagctgaca ttcctgctac tcaaattgag tcgcgcacgc aatcgagcaa gcgataccgg
    67441 cgcaggcttg gttggaagac tttcgatgaa ggtgtacaga gcctgtgctg tttctttgcg
    67501 tggtagctta ttgataacct ttaactgcaa aagaacctta tggtcaaagc gatatagttc
    67561 ggccagcttc ggttctgcat agaacaccac cgtatctttc tgctcgttgt agtccacgct
    67621 attgatgagg tgcaccatca gaagcgagat cttgttagag ccgtcgacgt tcttttcttc
    67681 atacgttctc tggaaagaca gagttgtacg catgatcttc aaaagactgt ttgtaagccg
    67741 gtcgcggagt gttttgcgga tctgtgacga tggatagcca caaaacttag caaatttcgt
    67801 gatgcttaac tcgacacgac cgttaggttc gccgtattct gccagcgaac gcacaacgcc
    67861 cacccacgtt ttgaaatcat gatccatgtc gagacgagga ccggttatct tgatatcgga
    67921 atagccttca gaacgggcta cttcgagctg gacaagctcc tttgaagcat cgatctcatt
    67981 tggcttgtta cgtttactgt attttgttcc cttaagcgtg gggacgaaca accccagtcg
    68041 catcaacgca attggttgga ctgtattgtt gctattaggg acaagttccc ctgtgtacaa
    68101 ttcaagggaa ccttcttcaa agttgtcgat gttatcttct acttctttgt tatttttacc
    68161 ttttttattt tttgtggaca tgtggacacc tttgtcattc aaccgctgac agcatacttg
    68221 atttgccgct gacagaatac caaaaacagt tggcagcata cggtgaaccg ctgacagact
    68281 atcaattacc gctgacagca tacatgaaca tgacttcaga ccagtcgtgg cgcggcttac
    68341 agcgatcggg gatcttattt gatctatata aggatctatc tatggatctc tttattagga
    68401 tctatcctgt ggatatgtga ataattaaaa caggcattta ctaccttcgg cgcacctggt
    68461 gggttatcgt tgcctcggct aacaatcaca gaaaaatgac atatggatct aaaacgcacg
    68521 cgctgggttc gtcgtcttga agacggctcc tacactatcg aatcaaattc caacctgaat
    68581 aagcagaagt tgctttgtga catctgcggt atagcggcga agtgcccgat ctacgaaacc
    68641 agaattaaac ttgataaggc tggtgtgaat tttcatttaa acagttgcat caggtacgtt
    68701 ccattactcg catttcgtaa accgatcatc ggattggatg ccccctactt caacacactc
    68761 cgttcaggtg tgacgtggcg agatcgttta tcaccagaca agctgatttg cctcgtatcc
    68821 gcagacacag gaaaaatcat ccgttttggg aaagtagaca aggtttactc aggcccagta
    68881 gacgaaatgt tgcggaaaca cagccggttt aatcatctct gtatgggtgg tgagaaaatc
    68941 gagaaggtag aagaagtgat ccgcaaatcc tacggacact ttctgaccaa agatagcctg
    69001 ctcaccgcaa tctacatcag acatgtaaaa cgtgagttcg acctcgaata ccacagcgaa
    69061 gaagagctta accttgttga cccacgtcca aaagctggcg tcataagcat aaacgcagcg
    69121 cgtaaaaagc ccactgacgc gctgtaaccc tccagatcgt atattggcgt agatagaatc
    69181 tacgcctcct caaaatagct ctcatagcgt tctacagtga tcctgtctta tttttagtca
    69241 tacagacaag caaagttgcg ccacaacaaa taggtatata cttacttata aattttgtat
    69301 attaagacgc tcgtttcatt cctaacatac cgttatgcat agttgtttac cttctcattg
    69361 ctcttaaaat ttgtatcaaa ataaccacaa aggaaaaaca catgactttg ccatacggcg
    69421 tcatttctga ctgccactac cacaaatggg atgcgttctc cacgacgaac gcagaggggc
    69481 ttaactccag acttgaaata cagttggaag caacgaaaga agcagccatc gccatgaaga
    69541 aggccggttg taagtacatg ttggttgccg gtgatacatt tcacgtccga ggaactgtgt
    69601 ccccttctgt tttgcattac gtaactgaaa cgtacaagtg gattatcaac gagcttgatc
    69661 tgacagtagt aatgctggcc ggtaatcacg atcttgaaac caacgattca gtatatagcg
    69721 ccaacgcagc agcatcgctg agttctatcg gcgtggtaat cgtatgtggc aaacgcccac
    69781 actcaataaa aattggtgat gtgactgtcc acctgattag ctggcgtaac aatcatgcgg
    69841 agcttatcag cgatctgaaa gcattacgta agagcgtaga aggtgataat catgacgttg
    69901 ttatccatac atccattaac aaagccattc caacaatgcc tgacgtcggt atcgatgcgc
    69961 aggagttaaa ggatatcggc tttcgtctcg tgcttagtgg gcattaccac aaccacaaag
    70021 aggtcattcc tggagttatc agtgtcggtg cactgaccca tcaaaattgg ggagatgttg
    70081 gatctctggc tggttacatg atcgtaaacc cggacggcag tttcagtcac tacgaaacca
    70141 gtgcgcctaa attcattaac ctggaagatg atgttgctga tgaccaaatt cgcggcaact
    70201 acgtgcgttt ccgcgccgta attgagaacg atgaagaagg cattaagtac cagaacatcc
    70261 tcaaaacaat gggcgcaaaa ggtgtcgtgt gcaacttcat ccgtaagtca tcaatgatgg
    70321 aagggacagc cagcacaact gaaaccagca aaatcgatag cctgggagag tcggtatctg
    70381 cttattgcaa gattgtccac gatactgacg gcggatttga tctgagcaag ttggatattt
    70441 tgtgtcagga aatcctcacc gaagcggaga gttcggaggc tgtgtgagac aaagtcgtta
    70501 tgggagcttt cgagactttg ccatcacgat gaaaagactt gaacgaggcc agacggtgat
    70561 gtttcacaag ccctacccgc cacaaggaaa tcccgtagcg ttttatcttg gaaggttaac
    70621 cagaaaaggc gtattgaggc gcagatcctt cccggcgcat acggagttca gattgaaaga
    70681 aggtcaaaag ctaacacacg gtatcaaagg tgttatatga agtttttaaa gctccaggtt
    70741 gagaatttta tggctatcgc cagcgcggag gtcgagttag atcagcgtgg tttagtgctc
    70801 attcagggtg ttaatagtga tgatagttcc gcatcaagta atggctctgg aaagtcaacg
    70861 ctaatgaata gcctgatgtg gtgtctttat ggcgaaacag ctcatggtgt gaagggtgac
    70921 gatgtgttgt ctaccgacca tgaaaagaac tgtcgtgttg cagtaaccat cgaggatgaa
    70981 ggcaagagat atgcgatcat tcgtcaccgt aaacacaaag agttcaaaaa tcggcttatc
    71041 gttcgtggtg aagacggcga tatgacgaaa ggcaaagatg cgctgacgca agagttcgtc
    71101 gagcgtctga tcggtgcatc taaagaggtt ttcatggctt ccatctatgc gagccaagaa
    71161 gctatgccag atttacctgg aatgtccgac aaaaacctca aaaccatcgt agaagaagcc
    71221 gctggcgttg acagactgac acgcgcctac gctattgctc gtgagcgagc taatgcagct
    71281 gccgcacgta tggatgtggt taaaaccaaa ttggagtcga caatctcgac cattgaggca
    71341 acacagtcag aaattgagtc cgcgaaagcc tcctctgaat catgggaaca agagcgttct
    71401 aaacgttatg acgatgccct ggctgggctg gcaagtgccg aagttgagtt aacggaagtt
    71461 gaacttgaga tccgcactct tcccgaacag atccgtgata ccgagaaggc aatcgaaagt
    71521 gagcgcaaaa agttagcctc aaaagaagaa catgacgcca agttgctcaa agttcgtggt
    71581 gcgataactg atattcgggc aagcatcaaa gctacagaaa atagtcaggc tgatgcaatg
    71641 aaccgcgcgc gtaattttaa gaccaaagca gaagaggttg gtactaaagt gggatcacca
    71701 tgccctactt gtggcaaagc ctactgcgaa gaagatctat caacggtgaa ggagaatttc
    71761 attgaacaag cacgtcagga aattggtcag gcgaagacac ttgcagaggc aatggctaaa
    71821 cacaaaacga atcttgagaa agcgttaagc attgagtctg cccttgttaa aacgacacct
    71881 gatgtaacgg ctatcattgc ccggattgaa gagcttacga aacaactctc atctttgcgt
    71941 catcgtgaga aggagattgt tgctattgag tctcttgtga ctcgtgctcg tactgaggtc
    72001 gatcgtatat caaaagagat taatccgttt attgctctta tcgccagaca cgaagataac
    72061 ctggtatcca gtaagtctac cttcaagtcc ttaaaagatg agttgaaggc tattcaggaa
    72121 caaacgttgc tattggaaaa agctcgtcag gtctactctc ctgccggggt gcgttctcat
    72181 attttgacgt ctgttacgcc tttcctgaat acacgcactg ccgagtatct caatacgttg
    72241 tctgacggga atattactgc tgagtggtcg acgatggatg tcaccaaaaa aggtgagtat
    72301 cgcgacaaat tcaacattag tgtgcagaag aaaggttcaa gtaagtcgtt ccagaccctc
    72361 tctggtggtg agaagcggaa ggttcgcatt gcgtgttctt tggcattgca ggatctggtt
    72421 agtaaccggg cgagtaaaaa catcgatttg tttatcggcg acgaaattga cgatgcactc
    72481 gatacagccg gtcttgaacg cctcatgggt attctggagt ccaaagctcg cgagcgaggt
    72541 actgtgctga ttatctccca taaagagatg aagtcatggt tccgggaaac tattacgctg
    72601 gaagttaaag aggggcgcag ctatgtcgtt taaattaagc cgctcgcagt ttttgcaggt
    72661 atttgcagtg atgcagtcga taaaactgat caatgggcat acttccaatg gtgcggctcc
    72721 acgtattctg tggggcagca acaatattga cggagtacaa tttgccgcgt tgcttggtct
    72781 aatatccgag acgccattga tgcaaagttt gaaatcacta ccacctggat gtattgcgcc
    72841 gatcctgatt aatccttttg ttgagggggg atatcttccc aacgtcgggc ctgggtttat
    72901 tgcatcccat gaaaatgaag atcttaacat taatagcgaa gggttctttg ggggaatggg
    72961 tgcgcatcac tgtatggctt tcaccaacct tattcgactt gccaataagc gggtggatag
    73021 tttggcatcg ccaggtgatg cttttactgg tttccttatc caaaggaggg ataaaaagta
    73081 cagtgcggac aaactacagt ttgttggtaa gtatggagaa atggtagaaa tcgaacttca
    73141 gctcccgcat gttttagcaa acgatagtgc agacagtcgg aggctgttgg gcatcatgcg
    73201 tcatttcata gcaagtggcg ttaaacatgc cgtagataaa cgtgtcacgc aggaaaatga
    73261 gtattcagac tttgcaaact ctccccaacc aacgttgcaa acggcaatag taaccaattc
    73321 gttggaggcg agattattgg aaaaccctat atggggaaca tggtaaggag actatatgag
    73381 taaaaaaatc agcgtagttg gtgttgatcc ctcaatgagc aactttgggc ttgctgtggg
    73441 cactttagac cttgaaacgg acgaacttga gattcacgac cttactcttg ttgagactaa
    73501 agcggggagt aacaaaaaga ccgttcgtgt gaacagtgac gatctgcgcc gagccagtga
    73561 aatatggcgt gttgcgaagc caatcattga taaggcaaat atggtttttt gtgagctacc
    73621 ggttgggagc caaaactctc gttcgcagac gtcttacggt atttgtatcg gtgtacttgc
    73681 gtgtgtggat aagccattga tccaggttac tccaaacgaa atcaagcatt ttgtcggcaa
    73741 taaacttact acatcgaaag aagagattat ccagtgggct acgaaaaaac actctaaagc
    73801 accgtggctg cgtcgtaagc aatctggaca ggatgttctc gtgaacaaaa acgaacattt
    73861 ggctgatgcg gtggctgcca tccataccgg tatgcaaaca gatcagttcc gccaggtgcg
    73921 cgatgttctt aagtctctca tttgatttca ttgataggta agtgcttatc tattaacatg
    73981 ggccactata tttagtggcc ctctttattt ggtgatacat gataagcatc gtaaaacgta
    74041 acggccaaac agagccgtta tccgaagaaa aatacaaccg cgtcgtaatg tatggcgtag
    74101 aaggcattcg tggtgtaagc gcatccgctg tagcaatggg agctgcggcc agcatttttg
    74161 atgggatgac caccagccag ttgcatgagg ctttggttaa atctgccgct gatttgatct
    74221 caccagaagc accaaattac tcacaggtgg ctgcccgcct gaacattttt aaaatccgca
    74281 aagatgcctt cggtcgttac gactatccga acttctacca acacattgtc aagaacgtta
    74341 acaagggcgt ttatgacaag gatttgctga cacattattc gtttgaagag atcgaagaac
    74401 tcggtaatta cattaagccg aaacgtgacg atctttttgg ctatgcagct acggtgcagt
    74461 tgcaaagcaa atacctcgtt caaaaccgtg ttactggtga gattcatgag gggccgcaac
    74521 atatctatat gctggtaggc atgtgtctgt tccagaattg ggaagacgac tgcgctggca
    74581 aaacacgtat ggagatggtc aaaggtttct atgacgttac aagtacgttc aaactgtctc
    74641 tgcccacacc aatcatggcc ggcgtccgta ctccaacccg tcagttctcc agttgtgtgc
    74701 tgattgagtc tggcgatagt ctgaaaggga ttagtgcagc ttcagccgca attatcgact
    74761 acgtttcacg tcgtgctgga attggtattg gttttggccg tatccgtgcg ctgggcagcg
    74821 agatccgcaa tggtgaagcc acccataccg gagttattcc attcctgaag catttccaga
    74881 cggctgttaa atcttgttcg caaggtggtg ttcgtggtgg cgcagcaaca gcgttttacc
    74941 cgatctggca tcttgaagtt gaaagtctgc tggtggtgaa aaataaccgt ggtattgatg
    75001 aaaaccgcgt tcgccatctt gattacggcg tcatgagtaa ccgtctaatg taccgtcgac
    75061 tcgtcagaag cgagaacatc actctgttca gcccgcatga tgtgcctgat atgtacgaag
    75121 ccttcttcac agaccaggag ctgtttgaaa agctgtacca taaatacgaa gccgatgatt
    75181 caattcgcaa gaagtcagta cctgccattg agctgttctc atctctgatg caggaacgag
    75241 cgtccacggg ccgaatttat attgcgaacg tcgatcatat taatgagcat ggcgctttca
    75301 ttcctgctct tgcacctgtc cgccagtcaa acctgtgtat ggagatcacc ctacctaccc
    75361 gtccactggc atttaccgac gacccgaacg gtgagatcgc gctatgcact ttatccgctt
    75421 ttaacctcgg agccatccgt tcactggagt ctcttaaaga ggtggcgttc tatgccgttg
    75481 ctgcactgga ttcgttactg gattatcaag actatccgat ggaggcagcc gaagtgcctg
    75541 ccaaagctcg tcgtagcctg gggatcggtg taaccaactt tgcttattac ctggcaaaga
    75601 atggcattcg ttattctgat accgctggta ataaactggt gcatgaaacg ttcgaagcta
    75661 tccagtatta ccttcttgat gccagctgcc aacttgctga agcaaaaggt gagtgtgact
    75721 ggtttgagca gaccaagtac gcaattggtc agttgccgat cgaccattac cgttcttcat
    75781 tagacgaaag tggcgaaacc aactttgagt taaagatgcc gtgggaagaa ctgcgtgaac
    75841 gtattgcaaa atacggtctt cgcaactcca cactgacggc acaaatgcca tgcgagactt
    75901 ccagccagat cactaattcc acaaacggca tcgaaccgcc tcgtggaccg gtgtcggtga
    75961 aatcttctaa ggacggcatc gttaagatgg tcgtgcctga gattgaaaaa ctgaaggaac
    76021 agtatgaata cctgtgggat atgccggaca accgcggcta tctgacaaag gtggcgatca
    76081 tccagaagtt ctttgaccag gctatttcgg ccaataccaa ctatgaccct tctcgctttg
    76141 aaggcgataa agtcccaatg atgacgctac tgtcagattt gcttctcgcc tacaagatgg
    76201 gagttaaaac gctttactac cacaacacca gagatggggc aggaaagcgt gatgacgacg
    76261 aaccgcagaa tccactgacg caagctgtag ccgtcgagcc agaagatgag tgcgacggag
    76321 cctgcaaaat ctgacatatg gtggggtgta tccccacctt ctctttgatt tgtaagcctt
    76381 gtttaaacaa ataggataac aacttgttta aacacatcaa aaagcaaaag gaaaaacaca
    76441 tgtcatattc aacgttccgt ttgggtgcta atgatgcaac caaagagcct atgttcctcg
    76501 gacaatctgt caacgtggca cgttacgatc agcaaaaata ccgtgatttt gaaaagttga
    76561 ttgaacgtca attgtctttc ttctggcggc cggaagaagt tgatatttcg agcgatcgta
    76621 tcgacttcaa cacgaagctg cgggaccacg aacgtcacat ttttctgagc aatctccgtt
    76681 atcaaacgtt actcgattca gttcagggac gtagcccaaa tgcaacgctg ctgccgctta
    76741 tctctattcc tgaactggaa acgtgggttg aaacatggtc tttctctgag actatccata
    76801 gccgcagcta cacccacatt attcgtggca tggtggacga tccgagcatt gtttttgacg
    76861 gtattgttac ggatgaagaa atcatcaacc gagcgatcag tatctctgct gaatatgaca
    76921 ggctttatgg gatgacctgc gagcgccagt cgttaggcga gaaggaattt gagcgtctgt
    76981 acgtaaatga atatggctgg gagccatatc ctttgcaccg tcagcttttc cgcacgttgg
    77041 tgtccattaa tgcgcttgag gcgatccgtt tctatgtaag ttttgcatgt acgtttgcct
    77101 ttggcgaacg gaagttgctt gagggtaaca ccaaaattat gcgctttatt gcccgtgatg
    77161 aagctctgca ttgcgaagga actgaacgca tgatccgctt catgcgtacc ggtcgcgaag
    77221 gtttattgtg gaaagagatt gctgctgatg aagaaaacgt catttacgac accatgaaat
    77281 cagtcgccga acaagaaatg aactgggccg agtatctctt caaagacggt tcgatgattg
    77341 gtttaaacgc ggatattttg aagacctatg taaaataccg caccaatctg gctatgaatc
    77401 gtcttggcct gaaggcttta tttccagagg ttaccaccga tccgctggtc tggatgaaca
    77461 agtggttgtt aaccgacaca ctgcaaattg caccacaaga ggcagagcaa agcacatatc
    77521 tggtaggtca gatcgattct accgtggaca aggcttctct aagccagttt gcagacctgt
    77581 gaaccgatac aaagcattat gtaacctggc aacgctgggc cacaatggat cacaagaatt
    77641 aagaaggaac aaaactagca tgaactttac caaactgact gaccacctga aacttgccac
    77701 cgatcgtctc attggattta agccagaacc atatgagttg catgaaggtc atggtgtagc
    77761 tactgaaagt atttacaaga tggtcgatca gtttcatgaa ctcttccagc atccgagacg
    77821 cgttatgccg acaccagagc tgcttcgtct ccgtgcaagc ctgattcatg aagaagctgt
    77881 agtggaaggt attccagctg caatgaatgg ggatattgag caactgctgg atgcaatggc
    77941 cgacttttta tacgttggtg ttggtacgat ggtcgccatc aaaggtggta tttctaccgg
    78001 catgacctat tacacgcagg aacagagcat tgatcgcttt atgcagacaa tttttgtgcc
    78061 cggtaacact gttttcgatg atatggcaat gccatttcag gaagctcgtg aggcgtcatg
    78121 tatgctcgaa gagctggcag ataaacttga gaacaagact gttaaggatt ctgagctgat
    78181 tcaggaactg cgccgtgtca tgaacaaaat ctatgtggcg tgcatgatga cctatcgact
    78241 ggctgatttc ctcggtatca atgtagtcga gctggttggc gaaattcatc ggtctaacat
    78301 gacaaaatta tggcctgctg atgtagagga acgtcgccag gctgtggcca actgcaaata
    78361 cgactcttca gacctgggat ttcgccatgc tgatggcacc gataagatga tcggttttcg
    78421 aatttccgat ggaaagattc tgaagtctcc aacctatagt gatgtcgatt tatcctcctt
    78481 tgttgagcaa gctaaagcct cagcaatgta cggaatgatc aaaaaataat tgtaggtgat
    78541 tatctactaa tgtatatttg atttgcgtat aaaactctgg aacgactatt ctttttggtg
    78601 gcctatggcc accatttttt atctatctgg tcttgtttta tcaatgaatg taaactcacg
    78661 tgataaataa ttgattactt atcttcgtga ggttttatgt cactcctttt gaatcgtgag
    78721 catacgaacg gtcaggtaac aaacgcatcg tatgcaaaag ttattgagac ggtgcttaaa
    78781 agcggcgtgc agactgatga tcgcacaggc actggtactt taagcacctg ctacgttccc
    78841 tcttactaca tgcttactgg tgggactgtg ccgcttattt ctggaaaggc ggtaaatctt
    78901 aagccactgc ttgtcgaact tgagtggtat ctgaaaggca cgggcaacat ccaatttctt
    78961 aaggataacg gcgttaagat ttgggatgca tgggccgatg agaatggcga tttggggccg
    79021 gtttacggta agcagtggcg tcgatgggaa gatacccgca tcgtgagcca tagtgaatat
    79081 ctgagcaaga tcgctacttt ccgtgaacgc gggtacaaag tcgagggata cctgggtatc
    79141 agtgaagatc gcgtagtgct gtcccgtgaa atcgatcagc tacagcgtat tgtcgacaca
    79201 ctgcgcacga accctaccga tcgtcgcatc atgcttaacg catggaacgt aggcgagctt
    79261 gaggatatga aactgccacc ttgccacttt gtcttctctt tgtggagtcg tgagcttgat
    79321 tttgaaaccc gtttaacgat ggcaactgac attggtcttc aacacagtcg cttcggttat
    79381 gagtctatct acaccaagat gctatacgat ctggagatgg acggcagtgt tactgaaact
    79441 gaactggatg aacttggaat ccccaaacgc atcctcaact cctgcctcgt acagcgtagc
    79501 gtagacactt ttgttggtat gccattcaat attgctggct atggcattct cactcatttt
    79561 ctcgcgaaga ttacgggtca catggccggt gcatttgtgc attttggctt tgacgtgcat
    79621 ttgtacaaca accacatgga aggtgtgtgt gagctaatga aacgacaggc tccagagcat
    79681 tcagatccgg tcgttatttt ccctcatgaa tggtctgagc tggatgattt caaatgggac
    79741 gatattctga ttcttggcta tgaccctcta ccgtggatca aggttccagt ggcggtgtga
    79801 tatggcaaga ggtatgtatg tcttatgcga aattgaaggt gtgctggcaa ataccagcca
    79861 tcgtaaatct gtacctgaca cggatgcagg ccagctcatt gccggtgatg aactcatttt
    79921 ccccaccagc cgtatgttgc gtggttttgc tcgctcaggg gctgaagtgg tgcttatcag
    79981 tagccgctct gaaactcttg aagcgcccac taaacgatgg ctgaaagatt ttggcgttga
    80041 ttacgactgg cttcatctcg taccgaatgg caccagttat gagaagcata ttaagcgcac
    80101 attagcggag cataaaggcg atctgcttat cgctgcgctg gtgcacgatc ctcgactccg
    80161 tgccgcttta gctgactctc atcatagacc ggtcatctat gaggtgagca aatgaagatg
    80221 atagctgctg ttggccgtaa ctatgagatc ggcatagcga acgaactgcc ctggcgttgt
    80281 tctaccgatc tgaagctatt taagagactc accaaaaacg ccactgtcgt tatggggcgt
    80341 aaaacgatgg aaagtctcaa acgccctctt ccagagcgtc ataacctcgt tttgacgcgc
    80401 tctcatggct ttgtaccaaa tggattctac cctgctggtg tggatgatgt gttgcgatta
    80461 ccagagcctg tgtgggtgat tggcggggaa caaatttact cgctattcat gccgcatgtt
    80521 gaagagattt ggctctccca catcggcgtt gatgtgccaa acgccgatgc attcttcccg
    80581 gcaagcatga tgcgtaattt aggctttgtg cctgttgaaa cagtttttac ccaacgagcc
    80641 agcgaggaag agcctggctt ttcgcagatc gtatacagaa ggtcgtaatg gattaccgga
    80701 ttgggatcac tggtgctcag ggcagtggga aaacaaccct ggcaaaatat atcgacaaac
    80761 attacggaat cccttacgtg gatgctggtg tcggaagttt gatgagccga ctcggtgttc
    80821 gagtaggtga gtctatgcct ctatatgagc ggcttcagat tcaaatggaa atcgcaaagc
    80881 atatagagct acttacgcgt ggtgttgaag gctttgttat cgatcgcaca cctgctgatg
    80941 ttatggccta cacgttggat ttggtcggcc ataccaatga agatcgatgt attgagttag
    81001 ccctcgatat cgaaaagttt tgccacaaaa ctgctatttc aaactttaac gccattgctg
    81061 gcctacgccc gggagtcgct ctctcagagc gagattactt gcgatcacaa cgagcatcat
    81121 tagaccgtct gtatgtcgct cgtattgatg cgttgatgtg cggggaactg acaaaaattc
    81181 acctgcatcc gcaaaggggg gatctgcaaa ccttcgtcgt ttccaaccga tatcgcacgg
    81241 ttgaagcaag agccagatca gtgatgagaa tgctagataa cgctgtagaa aagatagaaa
    81301 accggttctg tggtcgagtg accgttcatt agaaattgtt cgcctcttcg acattgcgac
    81361 aataaaactc tcaaaatggg ttaaggataa aaaatgttta gtgaaatgtt gcttgaagat
    81421 gaactggatc ggaaaacaac agaggctttg attcgtgtag cggacgaaca ttcccggtcg
    81481 cttatgagcg atcgagaggc tcgtctggct attcgtgcca tattcgaaac tgcgcagggg
    81541 cttgttggcg cacaagtagg tgaagccatt aacatcgcca tgtctcagtt cagtgaagac
    81601 agtaaaaagc ctctgtttcc tatgcatttg atgctggctg gtggcacggt gctttatatc
    81661 tctgtttgtc tggatagcaa ccaaatcaat attctcaaca ctgcgtcagg taagtggaaa
    81721 gatccgattg tctgtgaaac cagtgaagaa actttgaaaa aagcggctca atttgtacgt
    81781 agcgcactac ttaagggcgc taagaagttg taaggagttc tgatgacaac gattgttgca
    81841 ggcatcgata tcgagtctac gggactggat ttccttgctg gtcataaaat tattgaaatc
    81901 gcaattaccc gctatgaact ggagacacag agacatattg atagtctgga gatgcgtttt
    81961 aaccctcgca gaaacataga tccgaaagct caagccgttc atggcatttc attagaacag
    82021 ctcgcagctg aacctttgtt gtcaaatcat gctagcgaaa ttggcgctta tatggaggca
    82081 tgtagtgcgt gggttgctca taacggcgaa gcgtttgata taccatttat tagacacgag
    82141 ttttcagggt atggagtaag actgccagac gttcccgtta tagatactat gttatcggga
    82201 ttgtgggcca cagaagacgg taaacgtcct cgccttgaag aattggcctt ctctcttggc
    82261 tttatatacg atcatgccaa agcacatagt gccttatatg acacaaactt aatgatgcaa
    82321 tgcttcttta aggcacgtaa taaatacgga ttttttaaat taccctctga aattgtgtaa
    82381 aacaaaagcc tgctttaaag agagtttgaa gcaggctttc ttttaaagaa cagtcgcctt
    82441 tcaatcattt cctgcctgta tttaatactt ttccgcctga taggtttagt caaaatgtag
    82501 ccatcgaaac gcaaatgcaa ctaaacagaa ggagacttac atgagttcgg ttgaaaatgt
    82561 aatgacaaat gatgatctgg atgagctgac agccatgttg caatcacttg atgaaccagt
    82621 aaaaaaagct gcacaggttg aaaatactga tgatattgac gatctgcttc tcggcctcga
    82681 tgctggcgta gccatgagtt ctgatgatgt tgccgaagaa ctgttcaatg aagaaaaagc
    82741 aggtgatttc agctctgctt taaatgagtt ggagttagcg catgagccta taaacgtaat
    82801 taacgctgaa agtggtgaag ctgccgaaaa tgagccagaa cagttgggga tcattgaggt
    82861 tgaagattgt gttgaggtta atgatgaatt aaaagttcaa cagtcaaatg atagcaatac
    82921 aaataaaaaa actcgtgctg caagaggtcc tagttttact ttaagtgata aagatgattc
    82981 gtttttcaat aaagcaggct tagaaaaaga tattttctta gacgcttacg aaaacgcgcc
    83041 tgtcaaagcg aaggataaga tattaaacct tcttaattgg tttagcggag gtccagatat
    83101 tagtgtttac acggtaattt ccatgagaca ccttcttaca gaaaagaagg ctacaagtaa
    83161 tagtattaag attgctttaa tgagcaatcc agaaaaaccg tatccgctta acactgcgtc
    83221 aactcaggct gggcaaatga tggctgtatt tccagctaca ggaattgccg ttagagacgg
    83281 tggaaatcta acattgaacg aagaatcacc gatcgttaag aagtttgtcg cggagtacac
    83341 tattggatga cgttccccta ctgaaaataa agcccataga gagctttata gtgctgggta
    83401 agataaccca tacccagcac caaaaaaacg cgccagagag cttcttgttc gcatttctgg
    83461 cgcgttttat ttgattgcca tacataaaat caaatgcaaa aataggtatt tacttaccta
    83521 tcgagaaaga agatgattgc agccgaaaaa atcaaacagc gaaagcgcga caactctctt
    83581 cgtgacctct ggagaacacc tgattggctg ttttctgcca ttcaacgtta tcttggagtg
    83641 acatttgatg ttgacgttgc ctgcaacaag gacaacgcaa agctgcctaa tttcataggc
    83701 attgagcgtg atgctttgaa atctgaatgg ggacagccag gtacaattgc cttcctcaat
    83761 ccaccctact ccaaaatctc cccctggatt gatgctgcta tacgtgagca ggctcgcgga
    83821 gttactacag tgatgctaat tcctcaatcc ctcgatacaa agtggtatga gcgtgcaaca
    83881 gagtatgcga atgagacgat tattctgtct ggtggccgcg tagcgtttgt cgagcctgac
    83941 gtcaatttgg gtcaggtaga agtaaacatc aaccccggtg gcagtatgct cgttgttttt
    84001 cgaggattct gtcaggacgc tgggcactct ataagcaaga tccctttgga cgtcatgaaa
    84061 agtctgggag ggtatgatcc tgcgaatgtg atcaggaaaa aaagaccatc aaagaaggct
    84121 gcttagtttg ttctggcgtc tgtaattagc ctgcttctgt atatatataa ataactacat
    84181 attaattatt aatatacgga agcaggctgt tttgtatcag agactccaga cctgaacatc
    84241 actacagaat ccactagacc cccttcccag acgctttaaa atcgatttta tgaaccactt
    84301 taaggaaacc aacatgtcat acccgactaa tgtcgttgcg ctcgtagaca gcgattttct
    84361 ggcccaggct cgtgaaatga tgaaagatcg tgagcaggct ttcaacttgt acgagtgggc
    84421 aattaagtgc ttgcatcttg gggagcatcg cgaacttgtt gaacagcttt taggtgagtt
    84481 gatcaacgag gtgtttgcct tgaatgttca actacatggt cgagaaaata atcaatcgaa
    84541 atgatagata agtacaaact attcataaga tgaattgtaa gtgctaagat ctgatagttt
    84601 ccagtcgtag actggagact cgacctgatg ggtgggggta agcgtcactg gcgtcaggtt
    84661 taaaaaagct cactaccagc gtagaaccgg tgccgtttag gtgtcgggga agggggaacc
    84721 aaagtgagcg gagacaaggg tcactttatg attgtcgagt ctggggtgtt tcgagaggtt
    84781 gaatccagta ctccccttca taaagtgtgg gaagatctcg gttctggggt gctgtcatcc
    84841 ataacttccc aagtctaagc tggcagtaga cttagaccat agcttttcag gttatgaaac
    84901 gaccaggttg gtgaggaatt ttatactcac ctccctggga gagtattacc tgaaaagaca
    84961 acctctcact tcgttcgagg tgaacttcac tcacttcgtt cgttcagttc aggtttataa
    85021 aaacctgttc tgggaagtaa tttgtttatt ttaataatta ttaacacgca cgcgtgtgcg
    85081 cacgcgcgag gaaaaatcgg cgcggcgctt gattcaggag tttatatgac aacgaagaca
    85141 ccagcccgac cgcaagcaaa aactcgcaaa aaagacaaaa acaaaaattc tccccgcacc
    85201 aatcccacaa cgcctgtcgt agagttcaat ccccagctta aaaccgtgaa aatctttagt
    85261 gatggctctt gccttaaaaa tccgggtggc ccgggcggtt acggtatcgt tctccagtat
    85321 cgtggcgagg aacgcgagtt ctcagatggt tttcatagca ccaccaataa ccgcatggag
    85381 atgatggggg cacttatcgg gctggagcgt ttgaaatatc catgcaacgt tattttgcac
    85441 tctgatagcc agtatctgaa aaacggcatg acacagtgga tgaaatggtg gaaacgcaat
    85501 ggatggatga cttctgacaa aaaaccggta aagaatgttg atctgtggaa gcgtctggat
    85561 gaggccgcaa gtcgacataa tgttcgctgg aagtgggtta aaggtcacgc cgggcatcgt
    85621 gaaaatgaaa tatgtgatcg actcgcgaag atcgcagctt tttcagcagc agatatgcct
    85681 cacaagaaag atattggttt tgtttataac aaacagtaag taagtgttta cctatcatta
    85741 taaatcatgt atcttatcgg cgtcaggatg acaatgtgtc ggtaagacac agttccagga
    85801 tggaacgaga aaggcgg
//
LOCUS       DAZPJR010000500        11014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.guided.141, whole genome shotgun sequence.
ACCESSION   DAZPJR010000500 DAZPJR010000000
VERSION     DAZPJR010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.guided.141"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            110..1015
                     /locus_tag="ACHVB7_004805"
     CDS             110..1015
                     /locus_tag="ACHVB7_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR1916527.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACHVB7_004806"
                     /gene_synonym="mrx"
     CDS             1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACHVB7_004806"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR1916528.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACHVB7_004807"
     CDS             2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACHVB7_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR1916529.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3327..4091)
                     /locus_tag="ACHVB7_004808"
     CDS             complement(3327..4091)
                     /locus_tag="ACHVB7_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR1916530.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4318..4623)
                     /locus_tag="ACHVB7_004809"
     CDS             complement(4318..4623)
                     /locus_tag="ACHVB7_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR1916531.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="ACHVB7_004810"
     CDS             complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="ACHVB7_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR1916532.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            complement(5995..6198)
                     /locus_tag="ACHVB7_004811"
     CDS             complement(5995..6198)
                     /locus_tag="ACHVB7_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916533.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="ACHVB7_004812"
     CDS             complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="ACHVB7_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR1916534.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(7159..7506)
                     /locus_tag="ACHVB7_004813"
     CDS             complement(7159..7506)
                     /locus_tag="ACHVB7_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR1916535.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(7712..8500)
                     /locus_tag="ACHVB7_004814"
     CDS             complement(7712..8500)
                     /locus_tag="ACHVB7_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR1916536.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(8631..9104)
                     /gene="dfrA17"
                     /locus_tag="ACHVB7_004815"
     CDS             complement(8631..9104)
                     /gene="dfrA17"
                     /locus_tag="ACHVB7_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR1916537.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            9262..10275
                     /gene="intI1"
                     /locus_tag="ACHVB7_004816"
     CDS             9262..10275
                     /gene="intI1"
                     /locus_tag="ACHVB7_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1916538.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            10478..10828
                     /locus_tag="ACHVB7_004817"
                     /pseudo
     CDS             10478..10828
                     /locus_tag="ACHVB7_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
BASE COUNT         2289 a         3248 c         3186 g         2291 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     2401 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     2461 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     2521 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     2581 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     2641 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     2701 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     2761 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     2821 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     2881 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     2941 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3001 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3061 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3121 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3181 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     3241 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     3301 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     3361 cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac aaggcgcacc
     3421 tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg cagggctcgc
     3481 atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa accgcgcacc
     3541 ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt gagatacttc
     3601 acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc ggtgatcgct
     3661 gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca gtgcttcagg
     3721 cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg
     3781 tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt ccacttgccc
     3841 cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg ccgccagaac
     3901 cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata gatcgtcgta
     3961 tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata gctgatcgga
     4021 tagcgacaat accagcgcac cgcccacagg atcacatcac cctggaaatg gcgccacttg
     4081 aaatccgtca tcgttccgtc cgtccaatct ccgccaagca tgctcaagct tcacgatttt
     4141 tgcaacagag cccacacgag tattgagcat agtcgagatt ggtgcagatc acttctgata
     4201 ttgaactgtc aggagctggc tgcacaacag ccattacgcc caatcaactg gtgcagtcgt
     4261 cttctgaaaa tgacaatcca gttagggtat agctcaacct gacatagaag caaaaactca
     4321 accaccttct accaactctc cgaacagctc cttgaccttt gttttcgcat cagcaagtgc
     4381 agttctgcct tgttcagtga tgtcataaac acgccgttca cgtcgcccgg tgcgttcgtg
     4441 gcgtgaggtc agatagcctt ttttttccag gccgtgcagc atcgggtaca cggtgccagc
     4501 gctcatctcg tagccgtgtc ggcgtagctc ttcgatgatc cccagcccaa agacaggttc
     4561 ctcggctgca tggtgaagga tgtgcaggcg gatcaaaccg ccgtagaggt ctttgtcagt
     4621 cattttttgt gcctcacaga gcgacgctca acagccaccc agctgcaccg ctaccgagga
     4681 caaccagcca cggcgggagc ttccagaaca taagtgcgac aagggcaact aatgccaagc
     4741 cgaagtcttg cggctgaaag atggcgctag tccatacagg ctgatacagc gcggccagca
     4801 gcaagccgac tacagcggca ttgatcccgg ccagcgcagc ttggatgcct gtattgcggc
     4861 gcaaacgctc ccaaaatggc attgatccga cgaccagcaa gaacgagggc gcgaagatag
     4921 ccagcagaca cacaatgccg ccgatccagc ccgacggggc ggtgttcatc gaggcaccaa
     4981 gaaacgcggc gaacgtgaac aaagggccgg gcaccgcttg agctgccccg taccccgcga
     5041 gaaaggattc attgttgacc cagccggagg gcaccacttc ggcttgcagt aatggcagca
     5101 caacgtgacc accgccgaac accagtgatc cgacacgata gaaggaatcc accattgcca
     5161 tggtttgact tggcatcagt tcggccaaca ccggcaggcc aatcagcaag acaaagaaca
     5221 gcgagagcca aagcacgccg gcccggtgac tgaccgtgat aggtaggggg tcatgctcaa
     5281 caactttcgc tggcttgaac aataaccggc ctgcgatgcc tgcgatagca atcacgccaa
     5341 cctgtcccca cgcggacggc acaagtaaaa cgacgcaggt agcaattgcc atgatggtga
     5401 ctcgcagccc atccgtgcat aggttacgcg ccatgcccca tactgcttga gcgaccacgg
     5461 ccacagccac cacttttaag ccatgcaacg cgccctgcga gacgtaatcg ccatagctgg
     5521 agatgccgag cgcaaaaagg atcaaggcta tggcagacgg cagcgtgaag ccagcccaag
     5581 cagccagcgc cccgctgtat ccagcccgag acagtcctac cgctatgccg acctggctgc
     5641 ttgcaggccc tggcaagaac tgacaaagcg cgaccaagtc agcatagctc cgttcggaga
     5701 gccagcgccg ccgtgtgaca aattcggcgc ggaagtagcc caagtgcgca atggggccgc
     5761 caaaagatgt caatccaagc cgcagaaaaa taagaaagac cgaccatggt ctgctgtcat
     5821 cggtagggtt attcgtcata ctttcgcctt catgatctgc aacgagttga tcaataataa
     5881 gcgaaattcg ataacgaaat tcgatataaa tctagaaaaa aatacctcta tgtgtactac
     5941 gcagttttag ctgtggcttt cacaggagca cgcttactta cggcttagcg tgctttattt
     6001 tccgttttct gaggcgatcc ctaggagctc ggatctcagg acgaaggtct ccgcgaatgt
     6061 ccggtcgatc cgcgcgacgt cccaggcggg cgttcccttg gcggacatcc acgccgcagc
     6121 gtcgtgcatc agccgcacaa cctcgtcgat atcacccgag caggcgaccc gaacgttcgg
     6181 aggctcctcg ctgtccattc gctcccctgg cgcggtatga accgccgcct catagtgcag
     6241 tttgatcctg acgagcccag catgtctgcg cccaccttcg cggaacctga ccagggtccg
     6301 ctagcgggcg gccggaaggt gaatgctagg catgatctaa ccctcggtct ctggcgtcgc
     6361 gactgcgaaa tttcgcgagg gtttccgaga aggtgattgc gcttcgcaga tctccaggcg
     6421 cgtgggtgcg gacgtagtca gcgccattgc cgatcgcgtg aagttccgcc gcaaggctcg
     6481 ctggacccag atcctttaca ggaaggccaa cggtggcgcc caagaaggat ttccgcgaca
     6541 ccgagaccaa tagcggaagc cccaacgccg acttcagctt ttgaaggttc gacagcacgt
     6601 gcagcgatgt ttccggtgcg gggctcaaga aaaatcccat ccccggatcg aggatgagcc
     6661 ggtcggcagc gaccccgctc cgtcgcaagg cggaaacccg cgcctcgaag aaccgcacaa
     6721 tctcgtcgag cgcgtcttcg ggtcgaaggt gaccggtgcg ggtggcgatg ccatcccgct
     6781 gcgctgagtg cataaccacc agcctgcagt ccgcctcagc aatatcggga tagagcgcag
     6841 ggtcaggaaa tccttggata tcgttcaggt agcccacgcc gcgcttgagc gcatagcgct
     6901 gggtttccgg ttggaagctg tcgattgaaa cacggtgcat ctgatcggac agggcgtcta
     6961 agagcggcgc aatacgtctg atctcatcgg ccggcgatac aggcctcgcg tccggatggc
     7021 tggcggccgg tccgacatcc acgacgtctg atccgactcg cagcatttcg atcgccgcgg
     7081 tgacagcgcc ggcggggtct agccgccggc tctcatcgaa gaaggagtcc tcggtgagat
     7141 tcagaatgcc gaacaccgtc accatggcgt cggcctccgc agcgacttcc acgatgggga
     7201 tcgggcgagc aaaaaggcag caattatgag ccccatacct acaaagcccc acgcatcaag
     7261 cttttgccca tgaagcaacc aggcaatggc tgtaattatg acgacgccga gtcccgacca
     7321 gactgcataa gcaacaccga cagggatgga tttcagaacc agagaaagaa aataaaatgc
     7381 gatgccataa ccgattatga caacggcgga aggggcaagc ttagtaaagc cctcgctaga
     7441 ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc
     7501 tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
     7561 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcata
     7621 gttgagcggc gggcgcagcc cgtccgcttg aacgccgagt taggcatcag atgccctcgg
     7681 cgcgggtcga tgcacttttc gcacatgccg ctcaacgcaa gattctctca atcgttgctt
     7741 tggcatatcg aacgaacgcg gccgtctctt cgacgcgcat tgctaggtcg tcgtcctcgc
     7801 tacccaggta cgccgcgcgt gccttgcaga tgaggggccg atgctcggca ggcaaacgct
     7861 ccgataccca tgcggcagca acgtccttag gagcaatgag accagttgaa gcgctgtacc
     7921 aaatgcgagc aagagcaaga acgacgttcc gctcgtcacc cttccaatcc gactctgcat
     7981 tccactgggc aatagtgtcg aaaagcgcct tggagaaatg ctccttcggc accggctcga
     8041 aaaacgtggc tgcggatggg cctagaagcg caaggctgtg ttgcctcgcc ttggtcagca
     8101 aaatcgcaag atcgtgatcc agaacggcag gctcgaacgt tccggaaagg atgtcgtggc
     8161 ggagccactc accgaactga agctcacgcc gcgccggata gcgccaaggc actacttcgc
     8221 ttcgagcgac aacagttagc tccagcggtc gccatgttcc gccatcgcct ggcggtgatg
     8281 agactttcag caaatcgagc attagcgcct gccggagcga atcgttaggt gcggcgctga
     8341 cggtcacgag caagtctatg tcgctgtccg gcttcagccc tccatcgatc gcagatccga
     8401 acaggtggat tgtgtccagt gtcgcagcca gatggcgctc gatcaccgcg cgagcgtggg
     8461 acagctgctt gaaaacttgt gcagggaaaa attcacccat gatgcctaac gttaagttca
     8521 gcggcagctt ttaagttgcg gctttgtgga atacttttgc gcagcaaaac cacaaagacg
     8581 cgacttaaaa gctgtccaag gagcgaagcg actggtgctg caacgcattg ttagcctttt
     8641 ttccaaatct ggtatgtata atttatatta gacataaaaa actgttcaaa aaccaaattg
     8701 aaattctcag gcattatagg gaatttgata tcaccttcga cttcaacgtg aacagtagac
     8761 aaatgaatta tatctgcttt ttcaataagg ctattataga tttgaccccc gccagagaca
     8821 tatacatgat ctgtaacttt tgatagctct ttcaaagcat tttctattga aggaaaaact
     8881 aggacgtttt catttgagct tgaaattccg ttctttgaca ctactgcata tttgcgattt
     8941 ggaagaacac ccatagagtc aaatgttttt cttccgacaa ggagccattg attatatgtg
     9001 agcgctttaa agagtagttg ctcacctttt actgaccacg ggatatcagg accactaccg
     9061 attacgccat tttctgacac tgcagaaatc aatgatattt tcaatttaac tcccttaatg
     9121 gctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
     9181 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
     9241 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     9301 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
     9361 gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     9421 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag
     9481 gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg
     9541 ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg
     9601 ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat
     9661 cgtttgttcg cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg
     9721 cgggtcaagg atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc
     9781 aaggatcggg ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcagctgtcg
     9841 cgtgcacggg catggtggct gaaggaccag gccgagggcc gcagcggcgt tgcgcttccc
     9901 gacgcccttg agcggaagta tccgcgcgcc gggcattcct ggccgtggtt ctgggttttt
     9961 gcgcagcaca cgcattcgac cgatccacgg agcggtgtcg tgcgtcgcca tcacatgtat
    10021 gaccagacct ttcagcgcgc cttcaaacgt gccgtagaac aagcaggcat cacgaagccc
    10081 gccacaccgc acaccctccg ccactcgttc gcgacggcct tgctccgcag cggttacgac
    10141 attcgaaccg tgcaggatct gctcggccat tccgacgtct ctacgacgat gatttacacg
    10201 catgtgctga aagttggcgg tgccggagtg cgctcaccgc ttgatgcgct gccgcccctc
    10261 actagtgaga ggtagggcag cgcaagtcaa tcctggcgga ttcactaccc ctgcgcgaag
    10321 gccatcggtg ccgcatcgaa cggccggttg cggaaagtcc tccctgcgtc cgctgatggc
    10381 cggcagcagc ccgtcgttgc ctgatggatc caacccctcc gctgctatag tgcagtcggc
    10441 ttctgacgtt cagtgcagcc gtcttctgaa aacgacaatg gaggtggtag ccgagggtgt
    10501 ggaaacaccc gactgccttg cgtggttgcg gcaggcgggt tgcgacacgg tgcagggttt
    10561 cctgttcgcc aggccgatgc cggcggcggc cttcgtcggc ttcgtcaacc aatggaggaa
    10621 caccaccatg aacgccaatg aaccgagcac cagttgctgc gtgtgctgca aggaaatccc
    10681 gctcgatgcc gccttcacgc cggaaggggc cgagtacgtg gagcatttct gcgggctgga
    10741 gtgctatcag cgcttccagg cgcgggccag cactgcgacc gaaaccagcg tcaaaccgga
    10801 cgcttgtgat tcgccgccgt caggttgagg cataccctaa cctgatgtca gatgccatgt
    10861 gtaaattgcg tcaggatagg attgaatttt gaatttattg acatatctcg ttgaaggtca
    10921 tagagtcttc cctgacattt tgcagggaat tccatgactg gacagcgcat tgggggcact
    10981 gttgcaaagt tagcgatgag gcagcctttt gtct
//
LOCUS       DAZPJR010000501        10019 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.guided.154, whole genome shotgun sequence.
ACCESSION   DAZPJR010000501 DAZPJR010000000
VERSION     DAZPJR010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10019
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.guided.154"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..302
                     /locus_tag="ACHVB7_004818"
     CDS             <1..302
                     /locus_tag="ACHVB7_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HFR1916539.1"
                     /translation="SDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERS
                     QHANKRLARLLIAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(359..1816)
                     /gene="pepD"
                     /locus_tag="ACHVB7_004819"
     CDS             complement(359..1816)
                     /gene="pepD"
                     /locus_tag="ACHVB7_004819"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1916540.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2077..2535
                     /gene="gpt"
                     /locus_tag="ACHVB7_004820"
     CDS             2077..2535
                     /gene="gpt"
                     /locus_tag="ACHVB7_004820"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1916541.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2627..3871
                     /gene="frsA"
                     /locus_tag="ACHVB7_004821"
     CDS             2627..3871
                     /gene="frsA"
                     /locus_tag="ACHVB7_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1916542.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3929..4330
                     /gene="crl"
                     /locus_tag="ACHVB7_004822"
     CDS             3929..4330
                     /gene="crl"
                     /locus_tag="ACHVB7_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1916543.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4369..5424)
                     /gene="phoE"
                     /locus_tag="ACHVB7_004823"
     CDS             complement(4369..5424)
                     /gene="phoE"
                     /locus_tag="ACHVB7_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1916544.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            5712..6815
                     /gene="proB"
                     /locus_tag="ACHVB7_004824"
     CDS             5712..6815
                     /gene="proB"
                     /locus_tag="ACHVB7_004824"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1916545.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            6827..8080
                     /gene="proA"
                     /locus_tag="ACHVB7_004825"
     CDS             6827..8080
                     /gene="proA"
                     /locus_tag="ACHVB7_004825"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1916546.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            8195..8270
                     /locus_tag="ACHVB7_004826"
     tRNA            8195..8270
                     /locus_tag="ACHVB7_004826"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8228..8230,aa:Thr,seq:cgt)
     gene            8876..9034
                     /locus_tag="ACHVB7_004827"
     CDS             8876..9034
                     /locus_tag="ACHVB7_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916547.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2555 a         2365 c         2518 g         2581 t
ORIGIN      
        1 aatcggatgc aatccgttat gagacgttgc gttcgtcggg gccgggcggt caacatgtca
       61 ataaaaccga ctcggcggta cgcgccacgc atttggcatc cggtattagc gtgaaggttc
      121 agtcagagcg tagtcagcat gctaacaagc ggctggcacg attgctgatt gcctggaagc
      181 tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc gcagcggcga atgttccatc
      241 accagattga acgtggcaac ccgagacgga catttacagg gatggctttt atcgaaggat
      301 aatgaaggat gaaactgccg gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt
      361 acttcgccgg aatttctttc agcagttcag tcagcagtgt ccagtaatga cctacgcttt
      421 cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat agttggcccg atagaaacca
      481 tgtccatttc cggatacggt tttttgaaca gaccacattc caggcccgcg tggataatct
      541 ggatgttcgg cgttttgttg aacagacgct gataggtttc acgtaccaga tgcatcaccg
      601 gagaattagc gtccggctgc cagccaggat atgcgccttt cgcttcggtt ttcgcgccag
      661 ccagtttacc cagcgaatcc agcatgctca ccacgtagtc tttaccgctg tcgatcagag
      721 aacggatcag acagtgaatt tctacgttat tatcagtcat ggtcaccaca ccgacgttca
      781 gggaggtttc gaccacacct tttgccacgt ctgagttgcg gatcacaccg ttcggggtgg
      841 cgttcagcag acgaataaag gtatcgcgag atttcgcaat cagggcagct ttatcgttcg
      901 ctacagagtc cagcaacaag gccagatttt tctctttctc tgccagctcg tttttcagga
      961 tctcctgata ggtattcacc agagatttca ggacgtcgac tttatcagct gcgacagcaa
     1021 tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt gccgccgttg aaatcgataa
     1081 ggcgcagatc cagttcttct gcatgacccg ctaggaagcg caccagcagt ttgttggcat
     1141 tacccaggcc aacgtggatt tccccgccgg agtgaccccc tttcagacct tttaaggtta
     1201 acttgaaggt ttcaaaacca gctggaaccg cttcacgatc taaatgcagg ttggaggtga
     1261 agtcgatacc ccccgcacaa cccatgtaga tttcaccttc ttcttcggag tcggtgttaa
     1321 tcagaatatc agcctgcaac cagttgctct gtaagccgaa cgcaccgtcc ataccggctt
     1381 cttcggtcat ggtcagcagc acttccagcg ggccgtgaac cacgttttcg tcagccagaa
     1441 ccgccagcgc agacgccata ccaatgccgt tatctgcacc cagcgtggtg ccgcgcgctt
     1501 taacccattc gccatcaata taaggctgga taggatcttt cgtgaagtca tgcacggtgt
     1561 cgttattttt ctgcggcacc atatcgaggt gggcctgtaa gacgaccgat ttacgatttt
     1621 ccatacctgc ggtagcaggt ttacgaatca ggatattacc tacctgatcg cgttcgacat
     1681 ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc gagttgctct tcatgatagg
     1741 acgggtgagg aatagaacag attttggcaa aaatatccca cagcggctgt ggagataatt
     1801 gagacagttc agacacgtta agtctccttg tcgatcaccc gcaaaacagt attgcaggtc
     1861 acagggttag cagaaaatgt tgtcaacaca agacaggctt gcgagatatg tttgagaata
     1921 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     1981 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     2041 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     2101 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     2161 tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt
     2221 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
     2281 cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
     2341 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac
     2401 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
     2461 atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
     2521 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
     2581 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
     2641 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
     2701 ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc
     2761 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgtga
     2821 aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc gaacgtaccg acgatgattt
     2881 atacgatacg gtgattggct accgtggtgg caactggatt tatgagtggg ccatccaggc
     2941 gatggtgtgg caacaaaaag cctgtgcgga agaagatccg caactcagtg gtcgtcactg
     3001 gctgcatgcg gctacgttgt acaacattgc tgcctatcct catctgaaag gagatgacct
     3061 ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa gaagccgccc agcgtctgcc
     3121 gggcacgatg cggcagatgg agtttaccgt acccggcggt gcgcccatca ccggcttttt
     3181 gcatatgccg aaaggcgatg gcccgttccc gacagtatta atgtgtggtg gtctggatgc
     3241 gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg gcattgcgat
     3301 gctgactatt gatatgccgt cggtgggctt ttcatcaaaa tggaagctca cccaggactc
     3361 cagcctgttg catcagcacg ttttaaaggc tctgcctaac gtaccgtggg tggatcacac
     3421 tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct
     3481 tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata ccctgttgag
     3541 tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg cgagtcgttt
     3601 ggggatgcat gatgcttccg acgaagcgtt acgcgtggag ctgaatcgct attcattaaa
     3661 agtgcaagga ttgctggggc gtcgctgccc aacgccaatg ttatcaggct actggaagaa
     3721 cgatccgttc agcccggaag aggactcacg cttaatcacc tcatcatctg ctgacggtaa
     3781 attattagag atcccattta acccggtgta tcggaatttt gacaaaggtc ttcaggaaat
     3841 caccggctgg atcgaaaaac gcttgtgtta aaaatttgct aaaatttgcc aatttggtaa
     3901 aacagttgca tcacaacagg agattgcaat gacgttaccg agtggacacc cgaagagcag
     3961 attgatcaaa aaatttaccg cactaggccc gtatattcgt gaaggtaagt gcgaagataa
     4021 tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg
     4081 tgagttctgg ggctggtgga tggagcttga agcgcaggaa tcccgtttta cctacagtta
     4141 ccagtttggt ctgttcgata aagcaggcga ctggaagagt gttccggtaa aagacactga
     4201 agtggttgaa cgactggagc acaccctacg tgagtttcac gagaagctgc gtgaactgct
     4261 gacgacgctg aatctgaagc tggaaccggc ggatgatttt cgtgatgaac cggtgaagtt
     4321 aacggcgtga gtgaaatgtg ccggatgtat cacatccggc aatatccatt aaaactgata
     4381 ggtcatgcca accgcgacaa tatcatcatt attaatattc aatttgttat cgctatccag
     4441 ttggttgatt ttataatcaa caaacgctga catatttttg ttgaaataat acgtagcacc
     4501 gacgtcgata taattgacca gatcttcatc accgatacct tcaatatctt tccctttcga
     4561 taagacataa cccagcgatg gacgcagacc aaagtcaaac tggtattgag cgaccgcttc
     4621 aaagttctgt gtcttattgg caaagccgcc agttattggc gtcattttgc gtgtttcaga
     4681 atagaaagtt gccagataaa tattattggc atcgtatttc agacctgttg cccatgcttc
     4741 tgcacgcttg cctgtgccac ggctttgcag gttctgctcg ttggtgcgat ctgagttggt
     4801 ataggcccca ctaatggcga aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa
     4861 gccatcgccg ttttgctttt taacgtcgcg gttttcgttt ttcccttgat attgcagggt
     4921 taagttcagg ccatcgataa cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct
     4981 ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa tcgccaccaa attccgggaa
     5041 catatcggtc caggcttcca cgtcatacaa cgcgcccagg ttacgaccat aatcgaaaga
     5101 acccaaatct ttatatttca acccggcaaa agcgagacgc gttttctgct gtgcagtatc
     5161 actctccgct ttatttccgg caaactccgc ttcccaccga ccataaccag tcagttgatc
     5221 gttaatttgt gtttcacctt taaaaccaaa acggatataa ctctggtcgc catctttact
     5281 ggcgttatca ctcatataat gcatagcttt aactttgcca tagacatcca gtttattacc
     5341 gtctttatta tatatttctg cagcctgtac agatgcagat gccacaatgc ccatcaccac
     5401 taatgccaga gtgctctttt tcattttcat tcctgatttt aattaacgcg cgaatattca
     5461 gcgggagagc cccgttgaaa acaggaaagt ttttaacctg agattgttaa agatatatta
     5521 cagattaata atattcttaa aatgtggtaa tttattaaat ctgtaataaa agcgtaaaca
     5581 actgccgcta ggcttgctga tcccgcgcaa caaaacgcca tgctttgctc gcagatggtt
     5641 ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat catttttcct aaaattgaat
     5701 ggcagagaat catgagtgac agccagacgc tggtggtaaa actcggcacc agtgtgctaa
     5761 caggcggatc gcgccgtctg aaccgtgccc atattgttga acttgttcgc cagtgcgcgc
     5821 agttacatgc cgccgggcat cggattgtta ttgtgacgtc gggcgcgatc gccgccggac
     5881 gtgagcacct gggttacccg gaactgccag cgactatcgc ctcgaaacaa ctgctggcag
     5941 cggtagggca gagtcgactg attcaactgt gggaacagct gttttcgatt tatggcattc
     6001 acgtcgggca aatgctgctg acccgtgctg atatggaaga ccgtgaacgc ttcctgaacg
     6061 cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt tccggtaatc aatgagaacg
     6121 atgctgtcgc tacggcagag attaaagtcg gcgataacga taacctttct gcgctggcgg
     6181 cgattctggc gggtgccgat aaactgttgc tgctgaccga tcaaaaaggt ttgtataccg
     6241 ctgacccgcg cagcaatccg caggcagaac tgattaaaga tgtttacggc attgatgacg
     6301 cactgcgcgc gattgccggt aacagcgttt caggcctcgg aactggcggc atgagtacca
     6361 aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga caccattatt gccgcgggca
     6421 gcaagccggg cgttattggt gatgtgatgg aaggcatttc cgtcggtacg ctgttccatg
     6481 cccaggcgac tccgcttgaa aaccgtaaac gctggatttt cggtgcgccg ccggcgggtg
     6541 aaatcacggt agatgaaggg gcaactgccg ccattctgga acgcggcagc tccctgttgc
     6601 cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg tgaagtcatc cgcatttgca
     6661 acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta caacagcgat gcattacgcc
     6721 gtattgccgg acaccactcg caagaaattg atgcaatact gggatatgaa tacggcccgg
     6781 ttgccgttca ccgtgatgac atgattaccc gttaaggagc tggccgatgc tggaacaaat
     6841 gggcattgct gcgaagcaag cctcgtataa attagcgcaa ctctccagcc gcgaaaaaaa
     6901 tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa agcgaaatca tcctcaacgc
     6961 taacgcccag gatgttgctg acgcgcgagc caatggcctt agcgaagcga tgcttgaccg
     7021 tctggcactg acgcccgcac ggctgaaagg cattgccgac gatgtacgtc aggtgtgcaa
     7081 cctcgccgat ccggtggggc aggtaatcga tggcggcgta ctggacagcg gcctgcgtct
     7141 tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt tatgaagcgc gcccgaacgt
     7201 gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat gcggtgatcc tgcgcggtgg
     7261 caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg attcaggacg ccctgaaatc
     7321 ctgcggctta ccggcgggtg ccgtgcaggc gattgataat cctgaccgtg cgctggtcag
     7381 tgaaatgctg cgtatggata aatacatcga catgctgatc ccgcgtggtg gcgctggttt
     7441 gcataaactg tgccgtgaac agtcgacaat cccggtgatc acaggtggta taggcgtatg
     7501 ccatatttac gttgatgaaa gtgtagagat cgctgaagca ttaaaagtga tcgtcaacgc
     7561 gaaaactcag cgtccgagca catgtaatac ggttgaaacg ttgctggtga ataaaaacat
     7621 cgccgatagc ttcctgcccg cattaagcaa acaaatggcg gaaagcggcg tgacattaca
     7681 cgcagatgca gctgcactgg cgcagttgca ggcaggccct gcgaaggtgg ttgctgttaa
     7741 agccgaagag tatgacgatg agtttctgtc attagatttg aacgtcaaaa tcgtcagcga
     7801 tcttgacgat gccatcgccc atattcgtga acacggcaca caacactccg atgcgatcct
     7861 gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa gtggattcgt ccgctgttta
     7921 cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt ggtctgggtg cggaagtggc
     7981 ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg ctggaagcac tgaccactta
     8041 caagtggatc ggcattggtg attacaccat tcgtgcgtaa ataaaaccgg gtgatgcaaa
     8101 agtagccatt tgattcataa ggccattgac gcatcgcccg gttagtttta accttgtcca
     8161 ccgtgattca cgttcgtgaa catgtccttt cagggccgat atagctcagt tggtagagca
     8221 gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt atcggcacca ttaagaaaat
     8281 taataactta gcttaacttc tctaagaaat tgtatcttct gttatcactt acaaacgcct
     8341 atacgttgtt atgggctttg gtagtgtaac acgtatgggc gagtagttcc caggctcaac
     8401 ctcgtaacaa gttcctttga agctggaaga cgtgccaccg tctctaaacc ttctaccaaa
     8461 actcttagca agatgttcca cacctggcgc ggtgtccaat gatgctacac gtcgtgagac
     8521 gttgcagagg ataaacaggc taaatgatgg gaagagtttt aagtgcaata gtggattaaa
     8581 gcccacctct tcaaaaccta acttgatcgg gaaagaatca agctctagca cggtttcgtc
     8641 caccggagta caacgggtgc ctgataagag aagaactgct aagacttcat agcttttgtg
     8701 aaaaagccct ttttagtgga tacgcctacg gcgcttattt tttggtataa ccatcaaagg
     8761 cgctttatgg ctcctattgt cttgaaaatg tatctttact tgtattgtgt tttatgcgta
     8821 gtctccctgt gtagtatgta acttgttaat tttcatatga atacagaggc acacgatgaa
     8881 aaacattgca gcaatcaaac gtaataaccg caagattcac gctcgtaagt tcctgtctac
     8941 gccagaagga aaagcctggc tagagcgtaa acagagagag aacgaagaaa gaaaactcct
     9001 tagtgagttg aaatggctta gagaggattt ctgaaaatct ccagaacgca acaggacgca
     9061 ttctaacgct gttttcatta ctcggtatag ggataggtga tgtgtgttta atgcgtctgt
     9121 gcgcttgttt tgtgccttat tttgcatttt cataatgcgg aagagaacac ggaaaacctt
     9181 aaaattaatt attggggtat ttaaggctat tttcaggtga tttaagggta ttaaacttga
     9241 tttaattgat taaaatataa acagataatt atgtatctta ttgatttata aggttgtttt
     9301 taagtaaagg taaataaaaa ataaatagcc ataaatagct ataaatacat cttaatacct
     9361 atatcccttc ccttatggct agattgcgag aggtgcttaa cgcgattttc agcgcccccc
     9421 gatctggaaa atccagaatc cccaaatatc agcaaggaat tttcttgcgt aatcaacatc
     9481 gtttttacct attaaatcat tacacagaaa gcctttctgt aatgtctctc actaaacttt
     9541 cactaaacac gagaatgatc ggttttatgt cggatcagat gctagaaaca gcaccacgtc
     9601 tcacaagggc tgtaagcgat gaaaccagtg tttatgcggg tgcaggtcaa aatataggcc
     9661 aaaatccatt taacataata aatgttatat gtgctaagga acacatataa cgccttgaat
     9721 tgatgtatca gtggaaaagt gatggatttt ttacgtagga gcacgaaagg gcttctattg
     9781 acttttgtgt gctatgaaat ataattaaag ttttttgttt ttattgtgtg atgtggtggg
     9841 ttacacaggg gaatagatta aatggtgttc tgtttataca ggtttatttg ttagatcaac
     9901 aatttggcat ttctaaacag atgtttgaac gttgccaaat gtcttgacaa cacaagaaaa
     9961 gggcttgcta tgccaggcat tatgaggtgt actggcaata gcggacacta ccatttgtt
//
LOCUS       DAZPJR010000502         9932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.guided.155, whole genome shotgun sequence.
ACCESSION   DAZPJR010000502 DAZPJR010000000
VERSION     DAZPJR010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.guided.155"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(34..204)
                     /locus_tag="ACHVB7_004828"
                     /pseudo
     CDS             complement(34..204)
                     /locus_tag="ACHVB7_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHVB7_004829"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHVB7_004829"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1916548.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="ACHVB7_004830"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="ACHVB7_004830"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1916549.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="ACHVB7_004831"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="ACHVB7_004831"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1916550.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="ACHVB7_004832"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="ACHVB7_004832"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1916551.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="ACHVB7_004833"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="ACHVB7_004833"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1916552.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /locus_tag="ACHVB7_004834"
     CDS             5099..6232
                     /locus_tag="ACHVB7_004834"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1916553.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="ACHVB7_004835"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="ACHVB7_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1916554.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHVB7_004836"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHVB7_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1916555.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHVB7_004837"
     CDS             complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHVB7_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1916556.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHVB7_004838"
     CDS             complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHVB7_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1916557.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHVB7_004839"
     CDS             complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHVB7_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1916558.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8385..9413
                     /gene="epmB"
                     /locus_tag="ACHVB7_004840"
     CDS             8385..9413
                     /gene="epmB"
                     /locus_tag="ACHVB7_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1916559.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9803..9898
                     /gene="yjeJ"
                     /locus_tag="ACHVB7_004841"
                     /pseudo
     CDS             9803..9898
                     /gene="yjeJ"
                     /locus_tag="ACHVB7_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
BASE COUNT         2544 a         2520 c         2514 g         2354 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggt ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     6421 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     6541 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     7081 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccatccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attacctttg tgactatcgc aaaaaaacaa acggcacgac
     7321 ataactgtcg taccgttatt gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt
     7381 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     7441 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     7501 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     7561 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggagat
     7621 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
     7681 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     7741 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     7801 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     7861 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     7921 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     7981 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     8041 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     8101 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     8161 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     8221 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     8281 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     8341 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     8401 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     8461 ctggtgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     8521 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     8581 gcaatccgga cgatcctatt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     8641 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     8701 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     8761 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     8821 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     8881 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     8941 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     9001 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     9061 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     9121 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     9181 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     9241 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     9301 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     9361 ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta
     9421 aacagggaag ttaaacatta gcctgatgcg ctacgcttat caggcctaca ttgtgcattg
     9481 aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc gcatccggca
     9541 agagcaacaa acgcaagtac agcaacctgt cattaaactg ggaaatttaa tctttgcccg
     9601 ttgcataacc tttaattatt catatacatt gctgttgtta ctctttatat agccaacacc
     9661 aataattaac ctttcttttc tgtttatata gagtgagtac ggctatttat gttattttaa
     9721 caactattac taccaagccc atttatctgg cttataaatt aaaattaatt atttaattga
     9781 gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata ccggtgttat
     9841 tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc gcaataggta
     9901 atgactccaa cttattgata gtgttttatg tt
//
LOCUS       DAZPJR010000503         7705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.guided.193, whole genome shotgun sequence.
ACCESSION   DAZPJR010000503 DAZPJR010000000
VERSION     DAZPJR010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.guided.193"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            378..536
                     /locus_tag="ACHVB7_004842"
     CDS             378..536
                     /locus_tag="ACHVB7_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1916560.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="ACHVB7_004843"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="ACHVB7_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR1916561.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1872..2675
                     /locus_tag="ACHVB7_004844"
                     /note="aph(3'')-Ib"
     CDS             1872..2675
                     /locus_tag="ACHVB7_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR1916562.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2675..3511
                     /locus_tag="ACHVB7_004845"
     CDS             2675..3511
                     /locus_tag="ACHVB7_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR1916563.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            3817..4059
                     /locus_tag="ACHVB7_004846"
     CDS             3817..4059
                     /locus_tag="ACHVB7_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFR1916564.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(4091..4741)
                     /gene="tetR(A)"
                     /locus_tag="ACHVB7_004847"
     CDS             complement(4091..4741)
                     /gene="tetR(A)"
                     /locus_tag="ACHVB7_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR1916565.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            4847..6046
                     /gene="tet(A)"
                     /locus_tag="ACHVB7_004848"
     CDS             4847..6046
                     /gene="tet(A)"
                     /locus_tag="ACHVB7_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR1916566.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(6013..6201)
                     /locus_tag="ACHVB7_004849"
                     /pseudo
     CDS             complement(6013..6201)
                     /locus_tag="ACHVB7_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6245..6634)
                     /locus_tag="ACHVB7_004850"
     CDS             complement(6245..6634)
                     /locus_tag="ACHVB7_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916567.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6852..7292
                     /locus_tag="ACHVB7_004851"
     CDS             6852..7292
                     /locus_tag="ACHVB7_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR1916568.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(7351..>7705)
                     /locus_tag="ACHVB7_004852"
     CDS             complement(7351..>7705)
                     /locus_tag="ACHVB7_004852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916569.1"
                     /translation="TSLLHHLYITFTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAP
                     ATLHGHAINCVPVHRFRMGRTPETGLTPAEWCEFPRYALVRILMHAVKTEPARFWRVF
                     GWFVACFTGFPSETP"
BASE COUNT         1641 a         2187 c         2155 g         1722 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     1921 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1981 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     2041 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     2101 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     2161 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     2221 ttgatcaatg tcagtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     2281 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
     2341 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     2401 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     2461 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     2521 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     2581 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     2641 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     2701 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     2761 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     2821 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     2881 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     2941 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     3001 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     3061 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     3121 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     3181 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     3241 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     3301 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     3361 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     3421 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     3481 gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg
     3541 aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc
     3601 ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag
     3661 ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg
     3721 cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt
     3781 ccacgctaga tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc
     3841 gcccgcgagc gccaggcgca cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg
     3901 acgccgcagc aggagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg
     3961 acgctagggc agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg
     4021 aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca
     4081 gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc
     4141 ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg
     4201 cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg
     4261 tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag
     4321 tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc
     4381 agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg
     4441 catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc
     4501 cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg
     4561 ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa
     4621 gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc
     4681 cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca
     4741 tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa
     4801 tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacag
     4861 acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat
     4921 gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta
     4981 tggcattctg ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc
     5041 gctgtcggat cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt
     5101 cgactacgcc atcatggcga cggcgccttt cctttgggtt ctctatatcg ggcggatcgt
     5161 ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg atatcactga
     5221 tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt tcgggatggt
     5281 cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc cgttcttcgc
     5341 cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc cggagtcgca
     5401 caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt cgttccggtg
     5461 ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca tgcaacttgt
     5521 cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc actgggacgc
     5581 gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg cccaggcaat
     5641 gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat
     5701 tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga tggcgttccc
     5761 gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag caatgttgtc
     5821 caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg cgctcaccag
     5881 cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt ctataacaac
     5941 gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct
     6001 gcgtcgcggg ctttggagaa attcttcaaa ttcccgttgc acatagcccg gcaattcctt
     6061 tccctgctct gccataagcg cagcgaatgc cgggtaatac tcgtcaacga tctgatagag
     6121 aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc ggctggccgt
     6181 cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac atacagtcta
     6241 tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct agacattgcc
     6301 agccagtgcc cgtcactccc actcttacat tgttcgcagt atctaggtac atgttattgc
     6361 gtgcaccttc atgatgtata atgttattaa tgacagcaga aagatcgaca gaattaatat
     6421 tcttcgtacc tgggacagtt atcgggtgtc ggccagtgat gagaccaaag gatgcagtat
     6481 aagatgaacc actactatta tcatggccac cattaccacc accgttaaaa ctacctcccg
     6541 aacttccaga gccgctatga ccatcattgt aggggtcatt tggtcccatg tgaccaaaac
     6601 caccgacagt accgctattt aactctgtac tcataaaatt atcctctttg acttttaaaa
     6661 acagcaaaaa ctgtatttat aacaatgtgt ttatatacag tattcgaaat actacgcccg
     6721 tcaaaaccac accgtcaaca ggttttttgt gtatttttca cgcctcacgg gcagcctcag
     6781 accttttgtg ttaaaacagt aatatgttct gctgttacaa tacggtacac acaggagaca
     6841 ctgtcacttt tatggcatgg cacacagttc aagagacact gaaattaaca ggaaagtcgc
     6901 gctcgcaatt atatcgtgac atggctaaag gccttgtcag ctatcgcaca ggaaaaaatg
     6961 atcgccggga gttcgagacc agtgaactga ttcgggccta tggcgagctg cgcacttctg
     7021 agacaccaaa aaggcacaag gagggacact ccaaatctgt cgttagccag gcggaaaatg
     7081 ctcagctaga cgtcattcag cagaaactgg acgctctgca gcagactgtt accctaatgc
     7141 ttgaagacaa gatggcgcgg gaagcagaaa accgcctgcg aaatgaggag agggaacaac
     7201 tacaggccga aattagccgt ctggctgacg cgctggcaca ggaaaaaaag cggggattct
     7261 ggtcgcggct gtttcgctcc ggttcagagt aacagtgcag gtgactctga tttaccataa
     7321 catctgttat cagcaccgcc tgagaggccc tcagggcgtt tctgacggga aaccggtaaa
     7381 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg
     7441 catgagtatg cgcacaagtg catagcgcgg aaattcacac cattcagccg gggtaagtcc
     7501 tgtttccggc gttcttccca tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
     7561 gtgaagcgtt gccggcgcag ccggaaaacc cgctgtcagc acgttcagga agggggtgat
     7621 gtacaaatgg cacagaggat gaaagtgacg tatgagctga aggaatgatg taaaagtgat
     7681 gtataagtga tgtaaaagtg atgta
//
LOCUS       DAZPJR010000504         4988 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.guided.237, whole genome shotgun sequence.
ACCESSION   DAZPJR010000504 DAZPJR010000000
VERSION     DAZPJR010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.guided.237"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            complement(<1..290)
                     /gene="intI2"
                     /locus_tag="ACHVB7_004853"
     CDS             complement(<1..290)
                     /gene="intI2"
                     /locus_tag="ACHVB7_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HFR1916570.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPA"
     gene            622..1095
                     /gene="dfrA1"
                     /locus_tag="ACHVB7_004854"
     CDS             622..1095
                     /gene="dfrA1"
                     /locus_tag="ACHVB7_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1916571.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1190..1714
                     /gene="sat2"
                     /locus_tag="ACHVB7_004855"
     CDS             1190..1714
                     /gene="sat2"
                     /locus_tag="ACHVB7_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1916572.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1783..2280
                     /locus_tag="ACHVB7_004856"
     CDS             1783..2280
                     /locus_tag="ACHVB7_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916573.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            2341..2712
                     /locus_tag="ACHVB7_004857"
     CDS             2341..2712
                     /locus_tag="ACHVB7_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916574.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            3122..3499
                     /locus_tag="ACHVB7_004858"
     CDS             3122..3499
                     /locus_tag="ACHVB7_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1916575.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            3522..3674
                     /locus_tag="ACHVB7_004859"
                     /pseudo
     CDS             3522..3674
                     /locus_tag="ACHVB7_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(3730..>4988)
                     /locus_tag="ACHVB7_004860"
     CDS             complement(3730..>4988)
                     /locus_tag="ACHVB7_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR1916576.1"
                     /translation="PQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDHLEIR
                     ILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESWCFSF
                     DPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAGDGST
                     QHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRARKSS
                     QTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAFDELL
                     SILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLEVDAS
                     DGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGVNHPK
                     GLGELQVSREDMEGWAERVVREQFTH"
BASE COUNT         1390 a         1168 c         1084 g         1346 t
ORIGIN      
        1 cttgcaggga tataatcaat atcgcccaac ggctgttgta aaaacctgtt gtacaaaaaa
       61 gctagggcat ttaaagcgat tttctgcgtg tttatggcta catgtctgct gtttgctaag
      121 ctggataaaa acagcctgac ctcttcactg cccatggtct gaggatgacg ttttttgtga
      181 aacagaataa aacgcttaat ccagtgcagg taagtttttt cagttttcag cgcataacct
      241 ttttgtcgca tatccgtgcg tatagaattt aaaaatggac tgttagacat aaaacgctcc
      301 ttgtcttgca actgtctgcc tatacagcct attctagctg ggatttaaaa aagtgcctgt
      361 tttttacgcc tagagatgct tgtttaccgg tagagtttta atttaatgct aaataaatta
      421 aaatgttatg agttctttgg gtgagataat gtgcatcgtg caagcaggat agacggcatg
      481 cacgatttgt aataacagag tgtcttgtat ttttaaagaa agtctattta atacaagtga
      541 ttatattaat taacggtaag catcagcggg tgacaaaacg agcatgctta ctaataaaat
      601 gttaacctct gaggaagaat tgtgaaacta tcactaatgg tagctatatc gaagaatgga
      661 gttatcggga atggccctga tattccatgg agtgccaaag gtgaacagct cctgtttaaa
      721 gctattacct ataaccaatg gctgttggtt ggacgcaaga cttttgaatc aatgggagca
      781 ttacccaacc gaaagtatgc ggtcgtaaca cgttcaagtt ttacatctga caatgagaac
      841 gtattgatct ttccatcaat taaagatgct ttaaccaacc taaagaaaat aacggatcat
      901 gtcattgttt caggtggtgg ggagatatac aaaagcctga tcgatcaagt agatacacta
      961 catatatcta gaatagacat cgagccggaa ggtgatgttt actttcctga aatccccagc
     1021 aattttaggc cagtttttac ccaagacttc gcctctaaca taaattatag ttaccaaatc
     1081 tggcaaaagg gttaacaagt ggcagcaacg gattcgcaaa cctgtcacgc cttttgtacc
     1141 aaaagccgcg ccaggtttgc gatccgctgt gccaggcgtt aggcgtcata tgaagatttc
     1201 ggtgatccct gagcaggtgg cggaaacatt ggatgctgag aaccatttca ttgttcgtga
     1261 agtgttcgat gtgcacctat ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc
     1321 ctaccggaag gattacatct cggatgatga ctctgatgaa gactctgctt gctatggcgc
     1381 attcatcgac caagagcttg tcgggaagat tgaactcaac tcaacatgga acgatctagc
     1441 ctctatcgaa cacattgttg tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct
     1501 catcgaattt gcgaaaaagt gggcactaag cagacagctc cttggcatac gattagagac
     1561 acaaacgaac aatgtacctg cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg
     1621 cattgacctg ttcacgtata aaactagacc tcaagtctcg aacgaaacag cgatgtactg
     1681 gtactggttc tcgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc
     1741 gcggcgcggc ttaattcagg agttagagag ctggggaaga ctatgcgcga tctgttgaag
     1801 gtggttctaa gcctcgtact tgcgatggca tcggggcagg cacttgctga cctgccaatt
     1861 gttttagtgg atgaagctcg tcttccctat gactactccc catccaacta cgacatttct
     1921 ccaagcaact acgacaactc cataagcaat tacgacaata gtccatcaaa ttacgacaac
     1981 tctgagagca actacgataa tagttcatcc aattacgaca atagtcgcaa cggaaatcgt
     2041 aggcttatat atagcgcaaa tgggtctcgc actttcgccg gctactacgt cattgccaac
     2101 aatgggacaa cgaacttctt ttccacatct ggcaaaagga tgttctacac cccaaaaggg
     2161 gggcgcggcg tctatggcgg caaagatggg agcttctgcg gggcattggt cgtcataaat
     2221 ggccaatttt cgcttgccct gacagataac ggcctgaaga tcatgtatct aagcaactag
     2281 cctgctctct aataaaatgt taggcctcaa catctagtcg caagctgagg ggaaccacta
     2341 gtgtcatacg aacctccaag agacggttac acaaacgggt acattgttga tgtcatgtat
     2401 gacaatcgcc caagtaagta tccagctgtg ttcagaacgt acgtccgaat tccagactat
     2461 gtcgaactag ataacgcgga ggagctggcc gacgcaatca ttcgagaggt tgagttaaag
     2521 gaggcatggc tcacaagggt cttggggaac tcagccgatc ctgacgcttc tgtgggcaag
     2581 tgggaccggc gcctagcgca gtgcggtggt attgatgaga tgtcaagtgg ggtgcaggca
     2641 acatacttca gtccaaacgg tcagctggtc ggcttagtgg catacattga tgccgaatgg
     2701 atggatgaat agctctgacg ttacgattca ggtgagacaa ccagatgggc attgcgtaca
     2761 agctcagttg ccaccgatgt ggctacgagt ctgatcttct ttacctaggc caaggtatgg
     2821 cgatgcttcc tgagcaaata gtcggaacgt gcacatgctg cgacaatcta acgacgattt
     2881 ctataaatga aagctacgga gtatgctcac tttgtggggc taaggatcgt gtgatattta
     2941 caagctatca gcatgaggca aggccaagaa atccctgcta tctagagaga agtttcaaat
     3001 atcaatgccc aagatgccat gccttcagta tggagcctcc ggatctaccg gagatgcttt
     3061 gggactaatg ctccgctttg cctcgggtat ccgttcgacc tatgtttaag aggaaacgca
     3121 cgtgtctgcc gccaaagctc ttgcctatgc actttgtgac aagttggaaa gcaaaaaagt
     3181 tgatgaagtc gtacgaaatc ttgtttctag cggctggaat attagggaag tggcctggga
     3241 agcttcgtcc tttgatcatg aaatgcccta ctttgacacg gggcgggact tcgatattgg
     3301 atttcctgac acgctgtcat ccaagattga gtttttgacc gcgaatggat caatcgtcca
     3361 agcacgtgct cgcctgcact tcaagcaggc tctaattttt gccaaggcgt ccaaacatgt
     3421 cgccgcgctc aagaactcat tggattacta ctatggatcg tctgtaatcc cccctcaaaa
     3481 ccacggaggc tatttgtaga aaattccatt aatatgaact caaggtgttc cctacggacg
     3541 aatcagcgat gaaggtggtg tacctggcta tccaggcggc gaccaagaaa tggacgatgc
     3601 cgatccgcaa ctggaagccg gccatgaacc gttttatgat cgaatttggt gaccgtttaa
     3661 atggccacct ttaactgacc ggcaaaagca cttacacaaa agggtttaca ggctctttca
     3721 gtcattcctt taatgcgtaa attgctctct aaccactctc tcagcccacc cttccatatc
     3781 ctctctcgat acttgcagct cccccaaccc ctttggatga ttcacccctc tatgcccgtc
     3841 ttgtccatat aattgatcaa acaaactatt tggccaattc aatgagctct tcaccactcc
     3901 acgccgtatc ttttcaaaat cattccgcca tgtttcgcta tcaacgccac tcaacacttt
     3961 ggtagaaagc attttcaccc catccgatgc atccacctct agcaatacaa attcactgcc
     4021 attacgttgc accccaacgg ctttaatcac tcttggtgag ccatctttac acagatgtaa
     4081 tcggctacgc ccaacttttg gcaaaaccaa ggtttcttca aaaagcaccc gacatgcaaa
     4141 tttagttttt agaattgaaa gtagctcatc aaaagcagca aatcgattag caaaaataga
     4201 gttgtaattg gttgcatcct gcttcccacc cacatctgcc gctgctagga caccacctaa
     4261 gtgtggctca tcagtactaa ccaaattact attttcactt gttgttgctt cttctttttc
     4321 taaaatagtt tggctacttt ttcttgccct gcgagatttt tcaattcgac taggtctact
     4381 aaaacttatc catgtcggct cagcctctat gactcgtgtg tctgtgtcta tattggctgt
     4441 ttcctcatcg tccaactgta gatgctcatc atcaacaact gactctgttg aaaccgctat
     4501 gtgctgcgta ctaccatcac ctgcctcctt ttcctgaaaa gaggcatggc taatagctgt
     4561 tgtgctagga agcatagcgt tgatttctaa acctactatt tcctcaacta aataatcctt
     4621 atcctcgtta gaagaacgtc cttttacatg taatttccaa ccctgcatgg gcggagggtc
     4681 aaagctaaag caccagcttt caacgccgtt cttaatttct ctattttgtt gatagtgcct
     4741 aaaaatactt tcatacgaat ccataacatc ttgatccgaa aacaaccaaa ccaaaagctg
     4801 cactacggcc gactgctcta acgccccttt aggaaacgat gaactgggta agatccttat
     4861 ctctaaatga tctcgctcaa cctcatactg aacgtcgaac tcttgctgta aagcggtact
     4921 gctcaaacag cttcgacaga aataggagtt aattaaaaat aatgaccgag ctagctctaa
     4981 ctgtggga
//
LOCUS       DAZPJR010000505         1531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.guided.352, whole genome shotgun sequence.
ACCESSION   DAZPJR010000505 DAZPJR010000000
VERSION     DAZPJR010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.guided.352"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            <1..474
                     /locus_tag="ACHVB7_004861"
     CDS             <1..474
                     /locus_tag="ACHVB7_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1916577.1"
                     /translation="DDKFITTDYLQQCPTDREGLDLLRMKVEEGDVILVKKLDRLGRD
                     TADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGR
                     QEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            657..1517
                     /locus_tag="ACHVB7_004862"
     CDS             657..1517
                     /locus_tag="ACHVB7_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR1916578.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          408 a          340 c          404 g          379 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc caacagaccg ggaagggctg
       61 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa gctcgaccgt
      121 cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc tcagggcgtg
      181 gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca aatggtggtc
      241 accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg cacgaatgag
      301 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
      361 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
      421 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
      481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      601 cgctcatgag acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga
      661 gtattcaaca ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
      901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1021 gtgctgccat aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag
     1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1501 tgattaagca ttggtaactg tcagaccaag t
//
LOCUS       DAZPJR010000506         1521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1810332
            SAMN43277279-rid23489663.guided.353, whole genome shotgun sequence.
ACCESSION   DAZPJR010000506 DAZPJR010000000
VERSION     DAZPJR010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277279
            Sequence Read Archive: SRR30464548
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396899.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396899.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:41:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,462
            CDSs (with protein)               :: 4,462
            Genes (RNA)                       :: 100
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 135 of 301
            Pseudo Genes (incomplete)         :: 102 of 301
            Pseudo Genes (internal stop)      :: 96 of 301
            Pseudo Genes (multiple problems)  :: 31 of 301
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277279-rid23489663.guided.353"
                     /strain="M1810332"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-11-15"
                     /collected_by="FSS"
     gene            41..778
                     /gene="erm(B)"
                     /locus_tag="ACHVB7_004863"
     CDS             41..778
                     /gene="erm(B)"
                     /locus_tag="ACHVB7_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR1916579.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          550 a          263 c          250 g          458 t
ORIGIN      
        1 gtatttgttt atagatgtaa tcacttcagg agtaaattac atgaacaaaa atataaaata
       61 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      121 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      181 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      241 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      301 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      361 acaaattatt aaaaaagtgg tttttgaaag ccatgcgtct gacatctatc tgattgttga
      421 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      481 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaag
      541 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      601 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      661 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccgttac
      721 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      781 ctatgagtcg cttttgtaaa tttggaaagt tacacgttac taaagggaat gtagataaat
      841 tattaggtat actactgaca gcttccaagg agctaaagag gtccctagac tagcaagaag
      901 tacacaagaa gccttaaaga ttatagaaaa gcttttccat gaaatgctta acattattta
      961 atgttaacat gtggctttgt ggtaactacc acgagtggta acttgtaaca tgaaaaaagt
     1021 tacaagttac aagtggttta tttacgaaca taaaggctag gaaagcactt gataagtata
     1081 tcaagtgctt tttttccgtt cgattttact atccaagata ccgcttgacg tacctaatat
     1141 tttagtgtgc taagggcttt gctttttctg gttatagttc gttcaaaaaa gaccgcaagg
     1201 gtaaatgcac gatcatagat cgcccttgca gctttttaat ctctccactt ttttataacc
     1261 aagcaaacca ccaagcacac tgaaaaaata attaggaact aaagcgaagt gcgtatcttg
     1321 gataaatctt cactcgattt taaaagatta agcacccagc caaagcgcct aaaaaaataa
     1381 tttttttaaa gaaaggaagt aatttaaatg actacaattt taagcgacta caataaatct
     1441 atcctaattg aattactaaa caccaatcgc caaattatcg tcgttggtga tgctgccaac
     1501 ttactgattt agtgtatgat g
//
